Starting /dee2/code/volunteer_pipeline.sh ERR5296631
    current disk space = 1421063432
    free memory = 121344612 
ERR5296631 SRAfilesize
3c7bb8d3e81dcfc55d6a5e388c8b5aad  ERR5296631.sra
ERR5296631.sra file validated
ERR5296631 is paired end
ERR5296631 is conventional basespace
ERR5296631 read1 length is 59-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5296631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	59-76
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.92	38.0	38.0	38.0	32.0	38.0
2	36.67075	38.0	38.0	38.0	32.0	38.0
3	36.77475	38.0	38.0	38.0	32.0	38.0
4	36.69875	38.0	38.0	38.0	32.0	38.0
5	36.58925	38.0	38.0	38.0	32.0	38.0
6	37.51725	38.0	38.0	40.0	32.0	40.0
7	37.87575	38.0	38.0	40.0	38.0	40.0
8	38.31575	38.0	38.0	40.0	38.0	40.0
9	38.52775	40.0	38.0	40.0	38.0	40.0
10-11	38.567	40.0	38.0	40.0	38.0	40.0
12-13	38.503625	40.0	38.0	40.0	38.0	40.0
14-15	38.510875	40.0	38.0	40.0	38.0	40.0
16-17	38.523875000000004	40.0	38.0	40.0	38.0	40.0
18-19	38.53575	40.0	38.0	40.0	38.0	40.0
20-21	38.384125	40.0	38.0	40.0	38.0	40.0
22-23	38.37525	40.0	38.0	40.0	38.0	40.0
24-25	38.467625	40.0	38.0	40.0	38.0	40.0
26-27	38.418375	40.0	38.0	40.0	38.0	40.0
28-29	38.419250000000005	40.0	38.0	40.0	38.0	40.0
30-31	38.444874999999996	40.0	38.0	40.0	38.0	40.0
32-33	38.331	40.0	38.0	40.0	38.0	40.0
34-35	38.3365	40.0	38.0	40.0	38.0	40.0
36-37	38.311125	40.0	38.0	40.0	38.0	40.0
38-39	38.20975	40.0	38.0	40.0	38.0	40.0
40-41	38.173	40.0	38.0	40.0	38.0	40.0
42-43	38.283625	40.0	38.0	40.0	38.0	40.0
44-45	38.339124999999996	40.0	38.0	40.0	38.0	40.0
46-47	38.266875	40.0	38.0	40.0	38.0	40.0
48-49	38.277875	40.0	38.0	40.0	38.0	40.0
50-51	38.143	40.0	38.0	40.0	38.0	40.0
52-53	38.1695	40.0	38.0	40.0	38.0	40.0
54-55	38.203875	40.0	38.0	40.0	38.0	40.0
56-57	38.099875	40.0	38.0	40.0	38.0	40.0
58-59	38.142125	40.0	38.0	40.0	38.0	40.0
60-61	38.10654677176048	40.0	38.0	40.0	38.0	40.0
62-63	38.08568880285989	40.0	38.0	40.0	38.0	40.0
64-65	38.05693193193193	40.0	38.0	40.0	38.0	40.0
66-67	38.03365815753175	40.0	38.0	40.0	38.0	40.0
68-69	37.95651980741128	40.0	38.0	40.0	38.0	40.0
70-71	38.0061471118197	40.0	38.0	40.0	38.0	40.0
72-73	37.99779177794777	40.0	38.0	40.0	38.0	40.0
74-75	38.146365644570224	40.0	38.0	40.0	38.0	40.0
76	38.026391901663054	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	3.0
25	5.0
26	10.0
27	11.0
28	21.0
29	23.0
30	36.0
31	35.0
32	52.0
33	72.0
34	96.0
35	107.0
36	177.0
37	319.0
38	997.0
39	2035.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16516516516517	7.4574574574574575	3.6286286286286287	48.74874874874875
2	23.125	9.175	36.0	31.7
3	23.175	11.65	22.35	42.825
4	28.575	17.95	19.900000000000002	33.575
5	29.626846982218886	22.33909341347358	23.09040821437516	24.94365138993238
6	30.575000000000003	23.325000000000003	20.025000000000002	26.075
7	24.525	18.45	34.849999999999994	22.175
8	24.775	19.25	26.55	29.425
9	22.625	17.2	33.0	27.175
10-11	29.049999999999997	23.375	22.325	25.25
12-13	29.049999999999997	19.975	21.5625	29.4125
14-15	28.050000000000004	19.400000000000002	23.9875	28.5625
16-17	28.325	19.425	24.4125	27.8375
18-19	29.862499999999997	19.1375	21.425	29.575000000000003
20-21	28.8875	20.875	19.662499999999998	30.575000000000003
22-23	27.35	20.3125	22.625	29.7125
24-25	29.375	21.1125	19.787499999999998	29.725
26-27	28.6125	19.7	22.05	29.6375
28-29	27.712500000000002	21.025	21.45	29.812499999999996
30-31	27.4125	20.2125	21.2625	31.112499999999997
32-33	27.6625	21.1125	22.675	28.549999999999997
34-35	30.062499999999996	21.5625	21.099999999999998	27.275
36-37	31.1875	21.975	20.05	26.787499999999998
38-39	29.7125	22.162499999999998	21.099999999999998	27.025
40-41	28.623233712642243	20.657746654995623	21.945729648618233	28.773289983743904
42-43	29.791223902987873	18.952369046130766	22.802850356294538	28.453556694586823
44-45	28.4	20.075000000000003	24.2625	27.2625
46-47	30.775000000000002	19.7125	21.525	27.987499999999997
48-49	29.741217652206526	20.215026878359797	22.252781597699713	27.790973871733964
50-51	31.016377047130888	19.73996749593699	21.45268158519815	27.790973871733964
52-53	28.762500000000003	22.925	19.55	28.762500000000003
54-55	29.4375	19.2125	21.725	29.625
56-57	28.512500000000003	18.775	22.625	30.0875
58-59	27.35	20.6125	21.224999999999998	30.8125
60-61	30.12379642365887	20.407652869826183	20.382643491309242	29.085907215205705
62-63	30.998248686514884	20.052539404553414	21.203402551913936	27.745809357017766
64-65	30.205205205205203	19.744744744744743	21.15865865865866	28.89139139139139
66-67	30.988735919899874	18.448060075093867	21.551939924906133	29.011264080100123
68-69	28.959899749373434	19.26065162907268	21.791979949874687	29.987468671679196
70-71	28.17554858934169	20.489028213166144	21.16614420062696	30.169278996865206
72-73	29.442837378946045	19.83398314677399	22.5506225631996	28.172556911080367
74-75	31.15584588721504	16.197840287961604	22.73030262631649	29.916011198506865
76	28.27187274041938	0.0	28.9587852494577	42.76934201012293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	1.5
23	2.5
24	2.0
25	3.0
26	4.5
27	7.0
28	8.0
29	6.0
30	4.0
31	3.0
32	4.5
33	10.5
34	8.5
35	3.5
36	9.5
37	14.0
38	18.0
39	22.5
40	34.5
41	47.0
42	42.5
43	45.5
44	49.5
45	59.0
46	76.0
47	57.0
48	46.5
49	69.5
50	85.0
51	125.0
52	178.0
53	238.5
54	259.5
55	278.0
56	301.5
57	237.0
58	188.0
59	246.5
60	293.5
61	279.0
62	274.5
63	220.5
64	209.5
65	213.5
66	179.5
67	180.0
68	136.5
69	98.5
70	90.0
71	77.5
72	66.5
73	53.0
74	36.5
75	18.5
76	11.0
77	8.0
78	9.0
79	9.5
80	8.0
81	3.5
82	3.0
83	5.0
84	3.0
85	1.5
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.17500000000000002
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0375
42-43	0.0125
44-45	0.0
46-47	0.0
48-49	0.0125
50-51	0.0125
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.012514078338130395
68-69	0.01252975817566721
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
59	1.0
60	1.0
61	0.0
62	2.0
63	0.0
64	0.0
65	0.0
66	1.0
67	4.0
68	1.0
69	0.0
70	5.0
71	4.0
72	11.0
73	65.0
74	309.0
75	830.0
76	2766.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.57787810383748	37.974999999999994
2	16.16252821670429	17.9
3	7.178329571106095	11.924999999999999
4	1.9413092550790065	4.3
5	1.9413092550790065	5.375
6	0.8126410835214447	2.7
7	1.2189616252821671	4.725
8	0.4514672686230248	2.0
9	0.27088036117381487	1.35
>10	1.4446952595936793	11.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGC	25	0.625	No Hit
CTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGG	25	0.625	No Hit
CTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAG	24	0.6	No Hit
CCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTC	23	0.575	No Hit
CTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTG	22	0.5499999999999999	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCT	19	0.475	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAA	19	0.475	No Hit
CTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAG	18	0.44999999999999996	No Hit
CCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGG	17	0.42500000000000004	No Hit
CCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	17	0.42500000000000004	No Hit
CTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCT	17	0.42500000000000004	No Hit
GCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGC	16	0.4	No Hit
GTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGC	15	0.375	No Hit
GGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCT	15	0.375	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	14	0.35000000000000003	No Hit
GGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCA	13	0.325	No Hit
GTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCC	13	0.325	No Hit
GTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACG	12	0.3	No Hit
CTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGG	12	0.3	No Hit
GTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGT	12	0.3	No Hit
GCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTC	11	0.27499999999999997	No Hit
CCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGG	11	0.27499999999999997	No Hit
CCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCC	10	0.25	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	10	0.25	No Hit
GCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCT	10	0.25	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTT	10	0.25	No Hit
CTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGT	10	0.25	No Hit
CTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCT	10	0.25	No Hit
GGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	10	0.25	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	10	0.25	No Hit
GTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAG	10	0.25	No Hit
CTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCA	10	0.25	No Hit
GCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGG	9	0.22499999999999998	No Hit
CTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCT	9	0.22499999999999998	No Hit
CTGCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTA	9	0.22499999999999998	No Hit
GTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCA	9	0.22499999999999998	No Hit
GTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCA	9	0.22499999999999998	No Hit
GTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGT	9	0.22499999999999998	No Hit
GTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	8	0.2	No Hit
CCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGG	8	0.2	No Hit
GGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAG	8	0.2	No Hit
GGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCAC	8	0.2	No Hit
GGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCG	8	0.2	No Hit
GGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGT	8	0.2	No Hit
CCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCT	8	0.2	No Hit
CCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTC	8	0.2	No Hit
GTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGC	8	0.2	No Hit
GTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCC	8	0.2	No Hit
CCTGGTTAGAAAACTGGGGCCAGCACACAGGGGAAGGGTAAGCTGGTTTC	7	0.17500000000000002	No Hit
GTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTC	7	0.17500000000000002	No Hit
CTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCA	7	0.17500000000000002	No Hit
GCCAGGAGCTCACCTGCCTGCGTCACTGGGCACAGACGCCAGTGAGGCCAGAGGCCGGGCTGTGCTGGGGCCTG	7	0.17500000000000002	No Hit
CCAGCCTTGCACGGAGGGATCTGGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCCAGCAC	7	0.17500000000000002	No Hit
CTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGA	7	0.17500000000000002	No Hit
CTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCA	7	0.17500000000000002	No Hit
CCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	7	0.17500000000000002	No Hit
CCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCC	7	0.17500000000000002	No Hit
CTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAG	7	0.17500000000000002	No Hit
GTCACCATTTCCTGTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCC	7	0.17500000000000002	No Hit
CAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCA	7	0.17500000000000002	No Hit
CCCCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTT	7	0.17500000000000002	No Hit
CAGGATTATTAGCCTGCTGTGCCCGGGGAAAACATGCAGCACAGTGCATC	7	0.17500000000000002	No Hit
GTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAAT	7	0.17500000000000002	No Hit
CACCTGGTTAGAAAACTGGGGCCAGCACACAGGGGAAGGGTAAGCTGGTTTCATGATCGAATCAAGGCTCAGACA	7	0.17500000000000002	No Hit
CCCCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATC	7	0.17500000000000002	No Hit
GACGGGTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAACG	7	0.17500000000000002	No Hit
CTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGC	7	0.17500000000000002	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGAAAGGTGTCA	7	0.17500000000000002	No Hit
CTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGAGGAACCTTCCCCAAAC	7	0.17500000000000002	No Hit
CCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTC	7	0.17500000000000002	No Hit
GGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAG	7	0.17500000000000002	No Hit
CCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGC	7	0.17500000000000002	No Hit
GTGGTGGTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCT	7	0.17500000000000002	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	7	0.17500000000000002	No Hit
CTCCAGCTCGCCTCCCTCAATGGCCACTCCGTGCTCCAGCCATGCTGGCT	7	0.17500000000000002	No Hit
CCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGA	6	0.15	No Hit
GTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAACGAAAAG	6	0.15	No Hit
GGCCAAGAGGCTGCCGGAAGGGAAAAACAGGGCCTGGAATGGCCGACGTG	6	0.15	No Hit
CATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCT	6	0.15	No Hit
GGGGACAAGAGGAAAGAGGTGCCTGTGGGGGTGGAGGACAGGAAGGAAAA	6	0.15	No Hit
ACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCC	6	0.15	No Hit
CGTCTCTAAAGGTTGGAATGAATGGCCTAGAATCCGACCCAATAAGCCAA	6	0.15	No Hit
GGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCA	6	0.15	No Hit
GGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAGGC	6	0.15	No Hit
CTCTGATGCGTGTGTAACACAACATCCTCCTCCCAGTCGCCCCTGTAGCT	6	0.15	No Hit
GGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAG	6	0.15	No Hit
CCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCT	6	0.15	No Hit
CGGGTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAACGAA	6	0.15	No Hit
GTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCAC	6	0.15	No Hit
CCCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	6	0.15	No Hit
ATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTG	6	0.15	No Hit
GCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTC	6	0.15	No Hit
CCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCA	6	0.15	No Hit
CTGTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCCTGCTTGCAAGT	5	0.125	No Hit
CCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTC	5	0.125	No Hit
GTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCCTGCTTGCAAGTCC	5	0.125	No Hit
CCCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTC	5	0.125	No Hit
CCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGA	5	0.125	No Hit
CAGGAGGCTAAGTGGGGTGGAAGGGAGTGAGCTCTCGGACTCCCAGGAGT	5	0.125	No Hit
ATGAGATCTTAGGGCACACAGCTCCCCGCCCCTCTTCTGCCCTTCATCCT	5	0.125	No Hit
GTCCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGC	5	0.125	No Hit
CCCCACCGAAAAAGCCCATGGACAACGAAAAGCCCACTAGCTTGTCCAGT	5	0.125	No Hit
GCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	5	0.125	No Hit
ACCCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTG	5	0.125	No Hit
ATTTCCTGTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCCTGCTTG	5	0.125	No Hit
GTGAGAGCAGAGCCACCACAGGAAACCAGGAGGCTAAGTGGGGTGGAAGG	5	0.125	No Hit
CAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACA	5	0.125	No Hit
CCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGG	5	0.125	No Hit
CCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	5	0.125	No Hit
CTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCC	5	0.125	No Hit
AGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGG	5	0.125	No Hit
GTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATC	5	0.125	No Hit
GTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTG	5	0.125	No Hit
CAGCAGGATTTTGACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATAT	5	0.125	No Hit
GTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTCCGTG	5	0.125	No Hit
GGCAGGGCTGGGAGTACCAGCAGGCACTCAAGCGGCTTAAGTGTTCCATGACAGACTGGTATGAAGGTGGCCACA	5	0.125	No Hit
GCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGG	5	0.125	No Hit
CCTTGATCTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATG	5	0.125	No Hit
CCCAACTATGCACATAGCTTCAGCCTGCACAGATAGGGGAGTAGGGGACA	5	0.125	No Hit
CCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTG	5	0.125	No Hit
CCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCC	5	0.125	No Hit
GTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGC	5	0.125	No Hit
CCTTCCGTGACGGATGCCTGAGGAACCTTCCCCAAACTCTTCTGTCCCAT	5	0.125	No Hit
CTCCATTCTAAGTGCTGGACATGGGGTGGCCATAATCTGGAGCTGATGGC	5	0.125	No Hit
GGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATC	5	0.125	No Hit
GTGGAAGGGAGTGAGCTCTCGGACTCCCAGGAGTAAAAGCTTCCAAGTTG	5	0.125	No Hit
GCCTCACTTGTTCAGGCTCCCTCTGCCCTAGAAGTGAGAAGTCCAGAGCT	5	0.125	No Hit
CGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGCCTTGGCCCCCAGAGAGCCAATTTCACAATCC	5	0.125	No Hit
CCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	5	0.125	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTG	5	0.125	No Hit
CTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGG	5	0.125	No Hit
CCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCA	5	0.125	No Hit
GTTGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACAT	5	0.125	No Hit
ACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAG	5	0.125	No Hit
CTCAGAGCTTGGCTGGAGAAAGTTCGGGGCCTACAAAGGCGGTTGGGAGC	5	0.125	No Hit
AGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
ERR5296631 read2 length is 42-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5296631_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	42-76
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.01875	38.0	38.0	38.0	32.0	38.0
2	35.829	38.0	32.0	38.0	32.0	38.0
3	35.969	38.0	32.0	38.0	32.0	38.0
4	35.93475	38.0	32.0	38.0	32.0	38.0
5	35.7135	38.0	32.0	38.0	32.0	38.0
6	38.1835	40.0	38.0	40.0	38.0	40.0
7	38.242	40.0	38.0	40.0	38.0	40.0
8	38.22075	40.0	38.0	40.0	38.0	40.0
9	38.37275	40.0	38.0	40.0	38.0	40.0
10-11	38.324749999999995	40.0	38.0	40.0	38.0	40.0
12-13	38.409375	40.0	38.0	40.0	38.0	40.0
14-15	38.386125	40.0	38.0	40.0	38.0	40.0
16-17	38.3965	40.0	38.0	40.0	38.0	40.0
18-19	38.26925	40.0	38.0	40.0	38.0	40.0
20-21	38.335875	40.0	38.0	40.0	38.0	40.0
22-23	38.35825	40.0	38.0	40.0	38.0	40.0
24-25	38.26475	40.0	38.0	40.0	38.0	40.0
26-27	38.322125	40.0	38.0	40.0	38.0	40.0
28-29	38.152125	40.0	38.0	40.0	38.0	40.0
30-31	38.203875	40.0	38.0	40.0	38.0	40.0
32-33	38.277	40.0	38.0	40.0	38.0	40.0
34-35	38.182375	40.0	38.0	40.0	38.0	40.0
36-37	38.220749999999995	40.0	38.0	40.0	38.0	40.0
38-39	38.170500000000004	40.0	38.0	40.0	38.0	40.0
40-41	38.19325	40.0	38.0	40.0	38.0	40.0
42-43	38.14689303575894	40.0	38.0	40.0	38.0	40.0
44-45	38.12903225806451	40.0	38.0	40.0	38.0	40.0
46-47	38.144786196549134	40.0	38.0	40.0	38.0	40.0
48-49	38.15291322830708	40.0	38.0	40.0	38.0	40.0
50-51	38.07514378594649	40.0	38.0	40.0	38.0	40.0
52-53	38.040260065016255	40.0	38.0	40.0	38.0	40.0
54-55	37.98424606151538	40.0	38.0	40.0	38.0	40.0
56-57	37.979244811202804	40.0	38.0	40.0	38.0	40.0
58-59	37.914478619654915	39.0	38.0	40.0	38.0	40.0
60-61	37.89203608161577	38.0	38.0	40.0	38.0	40.0
62-63	37.90837319501869	38.0	38.0	40.0	38.0	40.0
64-65	37.93350864012021	38.0	38.0	40.0	38.0	40.0
66-67	37.914099674430254	39.0	38.0	40.0	38.0	40.0
68-69	37.902790606275204	39.0	38.0	40.0	38.0	40.0
70-71	37.89330343043319	39.0	38.0	40.0	38.0	40.0
72-73	38.01116555679553	38.0	38.0	40.0	38.0	40.0
74-75	38.07464097246809	38.0	38.0	40.0	38.0	40.0
76	37.746983408748115	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	4.0
21	1.0
22	1.0
23	10.0
24	7.0
25	9.0
26	13.0
27	16.0
28	23.0
29	34.0
30	28.0
31	34.0
32	56.0
33	61.0
34	80.0
35	105.0
36	150.0
37	318.0
38	1201.0
39	1846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.475	15.725	6.625	43.175000000000004
2	29.075	20.625	25.3	25.0
3	27.45686421605401	19.879969992498125	23.48087021755439	29.182295573893473
4	30.275000000000002	27.500000000000004	18.525	23.7
5	35.825	25.424999999999997	16.25	22.5
6	28.4	30.225	17.0	24.375
7	27.125	17.625	28.15	27.1
8	28.499999999999996	17.9	20.65	32.95
9	25.45	18.675	24.675	31.2
10-11	29.8875	24.075	20.05	25.9875
12-13	30.1375	17.75	20.962500000000002	31.15
14-15	29.4875	21.4	20.974999999999998	28.1375
16-17	30.7	20.3	20.7375	28.262500000000003
18-19	30.162499999999998	20.7125	20.25	28.875
20-21	29.9	19.575	20.45	30.075000000000003
22-23	30.191273909238653	18.98987373421678	21.427678459807474	29.391173896737094
24-25	30.23255813953488	21.117779444861213	19.317329332333085	29.332333083270818
26-27	29.678709838729837	19.6399549943743	20.215026878359797	30.466308288536066
28-29	31.775	18.587500000000002	20.150000000000002	29.4875
30-31	28.475	20.424999999999997	20.525	30.575000000000003
32-33	28.262500000000003	21.1375	20.525	30.075000000000003
34-35	29.603700462557818	20.827603450431305	19.814976872109014	29.753719214901864
36-37	28.766095761970245	20.31503937992249	20.19002375296912	30.728841105138144
38-39	30.370092523130783	19.929982495623904	19.379844961240313	30.320080020005
40-41	29.828728591073883	21.12764095511939	20.365045630703836	28.678584823102888
42-43	30.49893710141303	20.395148180567713	19.357258972114543	29.748655745904713
44-45	30.34396497811132	21.100687929956223	20.888055034396498	27.66729205753596
46-47	31.057764441110276	20.59264816204051	19.70492623155789	28.644661165291325
48-49	28.680520781171758	20.756134201301954	20.230345518277414	30.332999499248874
50-51	29.954954954954953	20.92092092092092	20.95845845845846	28.165665665665667
52-53	29.266766766766768	20.295295295295297	20.35785785785786	30.08008008008008
54-55	27.87787787787788	21.22122122122122	20.32032032032032	30.58058058058058
56-57	27.983487615711784	22.42932199149362	20.090067550662997	29.497122842131603
58-59	27.684605757196497	22.11514392991239	20.062578222778473	30.137672090112638
60-61	28.564275879334083	20.715984478658157	21.16660408061084	29.55313556139692
62-63	28.97406989853439	20.593761743705375	20.982086934736316	29.450081423023928
64-65	29.516411926835378	20.483588073164622	22.08719619143072	27.91280380856928
66-67	32.02204961162616	19.857178651966926	20.207967927837633	27.91280380856928
68-69	30.263157894736842	20.225563909774436	20.463659147869677	29.04761904761905
70-71	29.48701868807224	20.782641414774865	20.2056942179857	29.52464567916719
72-73	29.93831046204205	20.219060808258842	20.26941961475513	29.573209114943978
74-75	30.452618624367567	17.790236565021196	21.660057431970465	30.097087378640776
76	30.316742081447963	0.0	26.923076923076923	42.76018099547511
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	2.0
25	4.0
26	6.0
27	9.0
28	10.0
29	9.0
30	9.5
31	13.0
32	12.5
33	8.0
34	8.0
35	9.0
36	11.0
37	12.5
38	18.5
39	27.0
40	36.0
41	39.0
42	37.0
43	38.5
44	50.0
45	59.5
46	52.5
47	51.5
48	60.5
49	85.0
50	98.5
51	106.0
52	119.5
53	165.5
54	220.0
55	237.0
56	284.5
57	255.0
58	179.5
59	216.5
60	248.0
61	219.0
62	196.0
63	202.5
64	227.0
65	224.0
66	219.0
67	230.0
68	206.0
69	131.0
70	101.5
71	123.5
72	117.0
73	86.0
74	40.5
75	14.5
76	9.5
77	8.5
78	5.0
79	1.0
80	2.5
81	5.0
82	5.5
83	5.0
84	7.0
85	10.0
86	8.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0125
24-25	0.025
26-27	0.0125
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0125
36-37	0.0125
38-39	0.025
40-41	0.0125
42-43	0.025003125390673835
44-45	0.037509377344336084
46-47	0.0
48-49	0.12503125781445362
50-51	0.07501875468867217
52-53	0.07501875468867217
54-55	0.07501875468867217
56-57	0.05001250312578145
58-59	0.1000250062515629
60-61	0.050043788314775434
62-63	0.06259389083625438
64-65	0.050087653393438514
66-67	0.050087653393438514
68-69	0.025056376847907794
70-71	0.037612838515546636
72-73	0.0
74-75	0.013672409078479627
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	1.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	2.0
60	1.0
61	1.0
62	2.0
63	0.0
64	0.0
65	0.0
66	0.0
67	1.0
68	2.0
69	1.0
70	2.0
71	5.0
72	21.0
73	136.0
74	336.0
75	837.0
76	2652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.9913606911447	39.35
2	17.062634989200866	19.75
3	7.2570194384449245	12.6
4	2.4190064794816415	5.6000000000000005
5	1.7278617710583155	5.0
6	0.9935205183585313	3.45
7	0.6911447084233261	2.8000000000000003
8	0.6047516198704104	2.8000000000000003
9	0.4319654427645789	2.25
>10	0.8207343412526997	6.4
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTC	19	0.475	No Hit
CGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTT	18	0.44999999999999996	No Hit
CGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCT	18	0.44999999999999996	No Hit
CCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCT	17	0.42500000000000004	No Hit
CGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCAC	16	0.4	No Hit
CTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCA	14	0.35000000000000003	No Hit
GCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGC	14	0.35000000000000003	No Hit
GTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTC	13	0.325	No Hit
CAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAG	13	0.325	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	13	0.325	No Hit
CCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCC	13	0.325	No Hit
ACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGT	12	0.3	No Hit
CAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGA	12	0.3	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	12	0.3	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	11	0.27499999999999997	No Hit
CAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGG	11	0.27499999999999997	No Hit
CCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGG	10	0.25	No Hit
CAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAG	10	0.25	No Hit
GATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACC	10	0.25	No Hit
CCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCT	9	0.22499999999999998	No Hit
CTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAAC	9	0.22499999999999998	No Hit
CCCAGAAGCTGTTATTGTAGTCAGGATACGACAGGGGTGAGGCTACAGACAGGGGACTTGCAAGCAGGGAGGGC	9	0.22499999999999998	No Hit
AGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCAAGCCGG	9	0.22499999999999998	No Hit
GGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGA	9	0.22499999999999998	No Hit
CGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	9	0.22499999999999998	No Hit
CAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGC	9	0.22499999999999998	No Hit
GTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCA	9	0.22499999999999998	No Hit
GCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCA	9	0.22499999999999998	No Hit
CTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGAC	9	0.22499999999999998	No Hit
CTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGA	8	0.2	No Hit
CCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGC	8	0.2	No Hit
CTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGA	8	0.2	No Hit
GGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAG	8	0.2	No Hit
ATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTA	8	0.2	No Hit
GTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCT	8	0.2	No Hit
GCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	8	0.2	No Hit
CACCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTG	8	0.2	No Hit
ACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	8	0.2	No Hit
CTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCC	8	0.2	No Hit
GCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTAGAAGAAGTATGTC	8	0.2	No Hit
GTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTA	8	0.2	No Hit
TATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCC	8	0.2	No Hit
CAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGA	8	0.2	No Hit
TGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	7	0.17500000000000002	No Hit
GGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCC	7	0.17500000000000002	No Hit
GGATGACAGTCACCCTCTGTTTTCTGTGGTGGCTCTGTTTTCTGTGGTGC	7	0.17500000000000002	No Hit
CTTCATACCAGTCTGTCATGGAACACTTAAGCCGCTTGAGTGCCTGCTGG	7	0.17500000000000002	No Hit
CTTTAGGGCACGGGGACTTCTGGATTGTGAAATTGGCTCTCTGGGGGCCA	7	0.17500000000000002	No Hit
AGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGT	7	0.17500000000000002	No Hit
CCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCC	7	0.17500000000000002	No Hit
AGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCA	7	0.17500000000000002	No Hit
CTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCT	7	0.17500000000000002	No Hit
CCAGAAGCTGTTATTGTAGTCAGGATACGACAGGGGTGAGGCTACAGACA	7	0.17500000000000002	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTAGA	7	0.17500000000000002	No Hit
CCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAG	7	0.17500000000000002	No Hit
GGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATC	7	0.17500000000000002	No Hit
GCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGC	7	0.17500000000000002	No Hit
AGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTA	7	0.17500000000000002	No Hit
GGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCT	7	0.17500000000000002	No Hit
AGTGGATGCCAGGGACACCCAGAAGCTGTTATTGTAGTCAGGATACGACA	6	0.15	No Hit
AGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	6	0.15	No Hit
AAAAATTGTCTGAGCCTTGATTCGATCATGAAACCAGCTTACCCTTCCCC	6	0.15	No Hit
GGGACAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCC	6	0.15	No Hit
CCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATG	6	0.15	No Hit
GTTGGGATGAGTGAGTTAGGGCCCACGGATTACTCAAGACAGGACTTCAA	6	0.15	No Hit
ATTCTGCCTGGTGCATCTCTTGCCAATCAAATCAGTGCTGTCTGCAGTGTGATTGCTGCTTTAGTGGCACCAGGG	6	0.15	No Hit
CCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGC	6	0.15	No Hit
GGATTACTCAAGACAGGACTTCAAGTTGATTCAGGCGTGTTAGGGAGCTGTGATTGGATTTTGAGCAGGGCAGGG	6	0.15	No Hit
GCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCA	6	0.15	No Hit
TGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAG	6	0.15	No Hit
CAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGA	6	0.15	No Hit
CCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCA	6	0.15	No Hit
TGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACC	6	0.15	No Hit
TGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCG	6	0.15	No Hit
GAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	6	0.15	No Hit
CAGCAACACACCCTCTGGGAGCAGCCCCCTTGGGGAATCCCGGTCCTGGG	6	0.15	No Hit
GGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAG	6	0.15	No Hit
CCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAG	6	0.15	No Hit
GGCGTAGGGCTGGAGGGCAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCC	6	0.15	No Hit
GCCAGGAGTAATCAGGGCAGACCCTTTAGGGCACGGGGACTTCTGGATTG	6	0.15	No Hit
CTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTG	6	0.15	No Hit
GGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCA	6	0.15	No Hit
GAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAAC	5	0.125	No Hit
GCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCT	5	0.125	No Hit
GGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGT	5	0.125	No Hit
GTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCT	5	0.125	No Hit
CCGGCATTGCCCCCTCTGCCCCTGGCACCATTCCAGAACTGCCCACCTTCCACACTGAGGTAGCCGAGCCTCTCA	5	0.125	No Hit
GGCTGAGAGACGCAGGAAAGCGGGTCAAGGCGTAGGGCTGGAGGGCAGGG	5	0.125	No Hit
GTGAGTTAGGGCCCACGGATTACTCAAGACAGGACTTCAAGTTGATTCAG	5	0.125	No Hit
GGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	5	0.125	No Hit
CACACAGGCTCGCTCGTGCCCGGTGAGTGCTGAGGCTGCTCTGCACGTGG	5	0.125	No Hit
GACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	5	0.125	No Hit
CGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTG	5	0.125	No Hit
CTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCC	5	0.125	No Hit
CTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAG	5	0.125	No Hit
CTCCAAGAGAGTGCACGGTCCCTGCTGATTGAAAGAAGGATGAAGGGCAG	5	0.125	No Hit
GCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCA	5	0.125	No Hit
AACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAG	5	0.125	No Hit
GGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTG	5	0.125	No Hit
ACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGAC	5	0.125	No Hit
CCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGA	5	0.125	No Hit
GTGGGGAGCACCGCCACCTCTCCTCCTCCACTTGCCCCTCCTGTGGCACT	5	0.125	No Hit
GACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGC	5	0.125	No Hit
CCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAG	5	0.125	No Hit
CCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTG	5	0.125	No Hit
CGAGAGGAGGCCGTCCAGCAGATGGCGGATGCCCTGCAGTACCTGCAGAA	5	0.125	No Hit
ATTTCATCTTGGTGATTGCAGTCTACCCTCTGGCCTTTAAAAATTGTCTG	5	0.125	No Hit
CTTCACATTTGTTCCTTGTAAATTTCATCTTGGTGATTGCAGTCTACCCT	5	0.125	No Hit
GTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCT	5	0.125	No Hit
CATCAGTCCAAAGTCCAGCAGTTGTCCCTCCTGGAATCCGTTGGCTTCCC	5	0.125	No Hit
CTCTGTTTTCTGTGGTGCTGGATGCACCTCTGTTTTCTGTGGTGGCTCCA	5	0.125	No Hit
CCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAA	5	0.125	No Hit
TGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCA	5	0.125	No Hit
CTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGC	5	0.125	No Hit
CACCTTCCCCGCCAGACCCAGACTTGGGCCGTTGCTCTGACATGGACACA	5	0.125	No Hit
CGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCA	5	0.125	No Hit
GACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCT	5	0.125	No Hit
CCCAGACCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGG	5	0.125	No Hit
CAGGTGTTGAATGAACTGGATGGACTCTGCCAGATCCCTCCGTGCAAGGC	5	0.125	No Hit
GCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCA	5	0.125	No Hit
AGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCT	5	0.125	No Hit
CAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATCTTCACGGGCGCCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Read 3090924 spots for ERR5296631.sra
Written 3090924 spots for ERR5296631.sra
Rejected 3621 READS because READLEN < 1
Read 3090928 spots for ERR5296631.sra
Written 3090928 spots for ERR5296631.sra
SRR ids: ['ERR5296631.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_mjbndag3
ERR5296631.sra spots: 49454788
blocks: [[1, 3090924], [3090925, 6181848], [6181849, 9272772], [9272773, 12363696], [12363697, 15454620], [15454621, 18545544], [18545545, 21636468], [21636469, 24727392], [24727393, 27818316], [27818317, 30909240], [30909241, 34000164], [34000165, 37091088], [37091089, 40182012], [40182013, 43272936], [43272937, 46363860], [46363861, 49454788]]
ERR5296631 file size 9393876
ERR5296631 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 16 -o ERR5296631 ERR5296631_1.fastq ERR5296631_2.fastq
Input file:	ERR5296631_1.fastq
Paired file:	ERR5296631_2.fastq
trimmed:	ERR5296631-trimmed-pair1.fastq, ERR5296631-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Wed May  7 12:41:40 2025 >> started

Wed May  7 12:42:42 2025 >> done (61.487s)
49451867 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
49451867 (100.00%) read pairs available; of these:
    1363 ( 0.00%) trimmed read pairs available after processing
49450504 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 25	      31	  0.00%
 26	      37	  0.00%
 27	      33	  0.00%
 28	      37	  0.00%
 29	      61	  0.00%
 30	      84	  0.00%
 31	     101	  0.00%
 32	      97	  0.00%
 33	     144	  0.00%
 34	     167	  0.00%
 35	     166	  0.00%
 36	     210	  0.00%
 37	     230	  0.00%
 38	     278	  0.00%
 39	     354	  0.00%
 40	     383	  0.00%
 41	     442	  0.00%
 42	     522	  0.00%
 43	     585	  0.00%
 44	     572	  0.00%
 45	     673	  0.00%
 46	     829	  0.00%
 47	     918	  0.00%
 48	    1045	  0.00%
 49	    1360	  0.00%
 50	    2111	  0.00%
 51	    3100	  0.01%
 52	    3229	  0.01%
 53	    3114	  0.01%
 54	    3577	  0.01%
 55	    3917	  0.01%
 56	    4264	  0.01%
 57	    4458	  0.01%
 58	    5121	  0.01%
 59	    5973	  0.01%
 60	    6956	  0.01%
 61	    7590	  0.02%
 62	    8496	  0.02%
 63	    9710	  0.02%
 64	   11424	  0.02%
 65	   12969	  0.03%
 66	   14697	  0.03%
 67	   16767	  0.03%
 68	   19546	  0.04%
 69	   24607	  0.05%
 70	   33919	  0.07%
 71	   50162	  0.10%
 72	   98123	  0.20%
 73	  532157	  1.08%
 74	 4378644	  8.85%
 75	23370276	 47.26%
 76	20807601	 42.08%
49451867 reads passed initial QC


criterion=sequence-density
sequence-density=0.20
sequence-density-rank=1
fanout-score=14.76
fanout-score-rank=7
prefix-density=0.47
prefix-fanout=6.3
sequence=TTCCTCCTCTTC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=46.00
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=7.2
sequence=AAAAAAGAAAACAAAGACGATGGCCCCGGAAGGAATGCACAATTTGTTTTAGTTTACAGCACAGAGATCTTTCTCTCAATGGGAATTGTGCTCTTGGTTTCAGCAATAAGTGAAGGAAAAAAGATCTTGCCCTTTTGAAGTTCTGAGGGGAGGTGTAGGGTGTCCACGTTAGTACGGTTGGATAGGATATGCTCTCATGGTAACGCGTCCAAGTTGGAATGGTCTTCCAGTCTCCATGGCATCCACATGCTACTGGCGTTAGTTCCAGATCTTGAGGAAGCTATCCCAGGACCCTGTCGCCACAGCCATGCCATCGTCAGTCACGCCCAGGCAGCTGACGCGGTTGTCATGCCCAGCCAAGACACCTGCCCGGTCGGCTTTGAGTGCATCCCAGACGTTGCAGTTGAAGTCGTCGTACCCAGCAAGGAGGAGGCGCCCGCTCTTGGAGAAGGAGACAGAGGTGATCCCGCAGATGATGTTGTCATGGGAGTAAGTCATGAGCTCCTGGTCA


criterion=sequence-density
sequence-density=0.15
sequence-density-rank=1
fanout-score=3.36
fanout-score-rank=30
prefix-density=0.19
prefix-fanout=2.7
sequence=TGCACTCCAGCCTGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=56.07
fanout-score-rank=1
prefix-density=0.10
prefix-fanout=3.2
sequence=CCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACTTATATGATATGTCTCCATACCC
ERR5296631 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 07 12:43:10
                             Started mapping on |	May 07 12:43:10
                                    Finished on |	May 07 12:44:36
       Mapping speed, Million of reads per hour |	2070.08

                          Number of input reads |	49451867
                      Average input read length |	150
                                    UNIQUE READS:
                   Uniquely mapped reads number |	47538552
                        Uniquely mapped reads % |	96.13%
                          Average mapped length |	150.58
                       Number of splices: Total |	23453421
            Number of splices: Annotated (sjdb) |	23207908
                       Number of splices: GT/AG |	23237929
                       Number of splices: GC/AG |	168474
                       Number of splices: AT/AC |	24196
               Number of splices: Non-canonical |	22822
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.40
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1912236
             % of reads mapped to multiple loci |	3.87%
        Number of reads mapped to too many loci |	24
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.00%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1079	1079	1079
N_multimapping	1912236	1912236	1912236
N_noFeature	3258339	45038319	3674446
N_ambiguous	3767744	72709	1741784
UnstrandedReadsAssigned:40512469 PositiveStrandReadsAssigned:2427524 NegativeStrandReadsAssigned:42122322
Dataset is classified negative stranded
MeadianReadLen=76 20thPercentileLength=75 echo kmer=71
ERR5296631 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in paired-end mode
[quant] will process pair 1: ERR5296631-trimmed-pair1.fastq
                             ERR5296631-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 49,451,867 reads, 43,867,261 reads pseudoaligned
[quant] estimated average fragment length: 159.475
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,242 rounds

 180870 ERR5296631.ke.tsv
  58302 ERR5296631.se.tsv
 239172 total
==> ERR5296631.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	10	0	0
ENST00000448914.1	13	3	0	0
ENST00000415118.1	8	9	0	0
ENST00000631435.1	12	2	0	0
ENST00000632684.1	12	2	0	0
ENST00000454908.1	17	4.5	0	0
ENST00000390567.1	20	7.5	0	0
ENST00000603326.1	19	6.5	0	0
ENST00000414852.1	16	3.5	0	0

==> ERR5296631.se.tsv <==
ENSG00000223972	0
ENSG00000227232	115
ENSG00000278267	23
ENSG00000243485	3
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	2
ERR5296631 completed mapping pipeline successfully
