Starting /dee2/code/volunteer_pipeline.sh ERR5303527
    current disk space = 1374134928
    free memory = 123304336 
ERR5303527 SRAfilesize
9f4968d9979f3007dce66fbecc76eb25  ERR5303527.sra
ERR5303527.sra file validated
ERR5303527 is paired end
ERR5303527 is conventional basespace
ERR5303527 read1 length is 41-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5303527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41-76
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.9565	38.0	38.0	38.0	32.0	38.0
2	36.80625	38.0	38.0	38.0	32.0	38.0
3	36.74275	38.0	38.0	38.0	32.0	38.0
4	36.65225	38.0	38.0	38.0	32.0	38.0
5	36.62575	38.0	38.0	38.0	32.0	38.0
6	37.54925	38.0	38.0	40.0	32.0	40.0
7	37.9665	38.0	38.0	40.0	38.0	40.0
8	38.312	38.0	38.0	40.0	38.0	40.0
9	38.61325	40.0	38.0	40.0	38.0	40.0
10-11	38.579875	40.0	38.0	40.0	38.0	40.0
12-13	38.61425	40.0	38.0	40.0	38.0	40.0
14-15	38.544875000000005	40.0	38.0	40.0	38.0	40.0
16-17	38.552625	40.0	38.0	40.0	38.0	40.0
18-19	38.54675	40.0	38.0	40.0	38.0	40.0
20-21	38.576375	40.0	38.0	40.0	38.0	40.0
22-23	38.4955	40.0	38.0	40.0	38.0	40.0
24-25	38.561125000000004	40.0	38.0	40.0	38.0	40.0
26-27	38.490750000000006	40.0	38.0	40.0	38.0	40.0
28-29	38.448	40.0	38.0	40.0	38.0	40.0
30-31	38.436375	40.0	38.0	40.0	38.0	40.0
32-33	38.32425	40.0	38.0	40.0	38.0	40.0
34-35	38.38525	40.0	38.0	40.0	38.0	40.0
36-37	38.36225	40.0	38.0	40.0	38.0	40.0
38-39	38.3275	40.0	38.0	40.0	38.0	40.0
40-41	38.3185	40.0	38.0	40.0	38.0	40.0
42-43	38.264066016504124	40.0	38.0	40.0	38.0	40.0
44-45	38.3520880220055	40.0	38.0	40.0	38.0	40.0
46-47	38.297074268567144	40.0	38.0	40.0	38.0	40.0
48-49	38.30840090558041	40.0	38.0	40.0	38.0	40.0
50-51	38.288216162121586	40.0	38.0	40.0	38.0	40.0
52-53	38.32757757757758	40.0	38.0	40.0	38.0	40.0
54-55	38.345556946182725	40.0	38.0	40.0	38.0	40.0
56-57	38.270463078848564	40.0	38.0	40.0	38.0	40.0
58-59	38.2055068836045	40.0	38.0	40.0	38.0	40.0
60-61	38.211389236545685	40.0	38.0	40.0	38.0	40.0
62-63	38.17948944693636	40.0	38.0	40.0	38.0	40.0
64-65	38.157650889055844	40.0	38.0	40.0	38.0	40.0
66-67	38.09889696665831	40.0	38.0	40.0	38.0	40.0
68-69	38.09811448503497	40.0	38.0	40.0	38.0	40.0
70-71	38.086869570869155	40.0	38.0	40.0	38.0	40.0
72-73	38.079749174096726	40.0	38.0	40.0	38.0	40.0
74-75	38.183738019471015	40.0	38.0	40.0	38.0	40.0
76	37.98287671232877	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	4.0
26	8.0
27	7.0
28	25.0
29	12.0
30	29.0
31	41.0
32	43.0
33	71.0
34	96.0
35	85.0
36	186.0
37	349.0
38	990.0
39	2052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.75031273455092	8.756567425569177	5.253940455341506	52.2391793845384
2	22.3	9.4	35.275	33.025
3	23.3	11.075	21.6	44.025
4	27.075	17.8	19.35	35.775
5	28.045112781954888	20.927318295739347	23.909774436090224	27.11779448621554
6	29.875	21.95	22.475	25.7
7	24.224999999999998	17.2	34.949999999999996	23.625
8	23.799999999999997	19.025	28.249999999999996	28.925
9	23.075000000000003	16.5	32.7	27.725
10-11	25.837500000000002	22.7625	23.6875	27.712500000000002
12-13	28.1125	20.0	21.9	29.9875
14-15	27.8625	18.5	25.2875	28.349999999999998
16-17	25.5125	20.2375	25.4625	28.787499999999998
18-19	30.6875	18.9	23.2875	27.125
20-21	27.762500000000003	19.4625	21.425	31.35
22-23	26.787499999999998	19.7	23.0125	30.5
24-25	27.375	21.75	19.85	31.025000000000002
26-27	27.1375	19.6875	22.55	30.625000000000004
28-29	27.1125	19.1375	22.8125	30.9375
30-31	23.6375	19.8	24.1875	32.375
32-33	26.450000000000003	20.549999999999997	23.65	29.349999999999998
34-35	28.575	21.075	20.9	29.45
36-37	29.9375	21.55	21.375	27.1375
38-39	29.2875	20.5375	22.537499999999998	27.6375
40-41	28.65	19.25	22.3125	29.7875
42-43	28.81970492623156	18.017004251062765	24.381095273818453	28.782195548887223
44-45	27.906976744186046	20.192548137034258	23.768442110527634	28.132033008252062
46-47	29.40735183795949	20.78019504876219	20.905226306576644	28.907226806701676
48-49	29.064532266133064	20.860430215107552	22.52376188094047	27.55127563781891
50-51	28.971728796597446	20.302727045283962	20.878158618964225	29.847385539154363
52-53	28.015515515515517	21.834334334334336	21.333833833833836	28.816316316316314
54-55	28.74843554443054	20.075093867334168	21.576971214017522	29.599499374217775
56-57	26.382978723404253	18.56070087609512	22.07759699624531	32.97872340425532
58-59	28.410513141426787	19.511889862327912	20.67584480600751	31.401752190237797
60-61	29.299123904881103	21.526908635794744	19.28660826032541	29.887359198998748
62-63	29.402929027412693	20.065089498059834	21.354362248091125	29.177619226436352
64-65	32.569496619083395	19.408965689957427	21.362384172301528	26.659153518657654
66-67	30.04512409125094	19.917272499373276	21.49661569315618	28.5409877162196
68-69	30.639899623588455	19.84943538268507	21.091593475533248	28.419071518193224
70-71	27.508171988936386	19.298466180538092	22.755846115162182	30.43751571536334
72-73	27.4405665149216	19.663631765300963	23.57106727364694	29.324734446130503
74-75	30.480109739369	16.06310013717421	22.359396433470508	31.09739368998628
76	29.45205479452055	0.0	27.96803652968037	42.57990867579909
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	0.0
21	0.5
22	3.5
23	3.5
24	4.5
25	11.0
26	12.5
27	10.5
28	7.5
29	6.5
30	6.5
31	5.0
32	6.0
33	10.5
34	14.0
35	14.0
36	14.0
37	17.5
38	23.0
39	21.5
40	24.0
41	46.5
42	58.0
43	52.0
44	49.0
45	52.0
46	59.5
47	58.5
48	57.5
49	73.0
50	92.0
51	136.5
52	169.5
53	231.0
54	268.5
55	252.5
56	252.5
57	199.0
58	172.0
59	241.5
60	285.5
61	263.0
62	261.0
63	214.5
64	218.0
65	243.0
66	220.0
67	225.0
68	158.0
69	105.5
70	100.0
71	72.0
72	44.5
73	29.5
74	25.0
75	20.0
76	12.5
77	9.0
78	6.5
79	6.0
80	12.0
81	8.0
82	5.5
83	8.0
84	4.0
85	3.0
86	3.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.25
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
41	1.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	2.0
49	0.0
50	0.0
51	1.0
52	0.0
53	1.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	1.0
63	1.0
64	0.0
65	4.0
66	0.0
67	3.0
68	2.0
69	6.0
70	2.0
71	4.0
72	36.0
73	71.0
74	440.0
75	797.0
76	2628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.79172229639519	16.400000000000002
2	21.82910547396529	16.35
3	14.352469959946596	16.125
4	6.608811748998665	9.9
5	4.2723631508678235	8.0
6	2.069425901201602	4.65
7	2.202937249666222	5.775
8	1.268357810413885	3.8
9	0.6675567423230975	2.25
>10	2.9372496662216285	16.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	42	1.05	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCT	31	0.775	No Hit
CCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGG	28	0.7000000000000001	No Hit
CTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTG	25	0.625	No Hit
GCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGC	24	0.6	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	23	0.575	No Hit
CTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGC	18	0.44999999999999996	No Hit
CTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCA	18	0.44999999999999996	No Hit
CTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGT	17	0.42500000000000004	No Hit
CCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTC	17	0.42500000000000004	No Hit
GTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCA	16	0.4	No Hit
CCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTC	16	0.4	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAA	16	0.4	No Hit
GTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	15	0.375	No Hit
CCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGG	15	0.375	No Hit
GTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGT	15	0.375	No Hit
CCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGGAGGGATGGAGTCTG	15	0.375	No Hit
GGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	14	0.35000000000000003	No Hit
CCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	14	0.35000000000000003	No Hit
ACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAG	14	0.35000000000000003	No Hit
GCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTT	13	0.325	No Hit
CTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCA	13	0.325	No Hit
GTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGT	13	0.325	No Hit
GGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCA	13	0.325	No Hit
CCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTC	13	0.325	No Hit
CTCCAATGGCCTGCACCTGGCTCCGGCTCTGCTCTACCTGCTGAAGATGT	12	0.3	No Hit
GTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGT	12	0.3	No Hit
GGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCA	12	0.3	No Hit
CCCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCC	12	0.3	No Hit
CCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCC	12	0.3	No Hit
CTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCA	12	0.3	No Hit
GTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGG	12	0.3	No Hit
CTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGT	12	0.3	No Hit
GTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGA	12	0.3	No Hit
GTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCA	11	0.27499999999999997	No Hit
GCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCC	11	0.27499999999999997	No Hit
CCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTC	11	0.27499999999999997	No Hit
CTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCT	11	0.27499999999999997	No Hit
GGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCC	10	0.25	No Hit
GCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	10	0.25	No Hit
GTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGC	10	0.25	No Hit
CCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTC	10	0.25	No Hit
CTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAG	10	0.25	No Hit
TACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGAT	10	0.25	No Hit
GCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCT	9	0.22499999999999998	No Hit
GTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAAT	9	0.22499999999999998	No Hit
CCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCC	9	0.22499999999999998	No Hit
CATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTG	9	0.22499999999999998	No Hit
CCCCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATC	9	0.22499999999999998	No Hit
CCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCC	9	0.22499999999999998	No Hit
GGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCT	9	0.22499999999999998	No Hit
CGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCC	9	0.22499999999999998	No Hit
CCCAAACTCTTCTGTCCCATCCCTGCCCTGCTCAAAATCCAATCACAGCTCCCTAACACGCCTGAATCAACTTGA	9	0.22499999999999998	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	9	0.22499999999999998	No Hit
TGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGTACATG	8	0.2	No Hit
CAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGCAGGACCCCC	8	0.2	No Hit
CTTCTACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGC	8	0.2	No Hit
CCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCC	8	0.2	No Hit
GCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCA	8	0.2	No Hit
GGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAG	8	0.2	No Hit
CTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGAGGAACCTTCCCCAAAC	8	0.2	No Hit
CCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGG	8	0.2	No Hit
CCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCT	8	0.2	No Hit
GCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	8	0.2	No Hit
GTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCT	8	0.2	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTG	8	0.2	No Hit
GGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCT	8	0.2	No Hit
GTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGC	8	0.2	No Hit
GGGCTGAGGACCTCTGGTGGCGGCCCAGGGCTTCCAGCATGTGCCCTAGG	8	0.2	No Hit
GGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCA	8	0.2	No Hit
CTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGG	8	0.2	No Hit
CCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCA	8	0.2	No Hit
CTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGA	8	0.2	No Hit
CCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGG	7	0.17500000000000002	No Hit
GGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAG	7	0.17500000000000002	No Hit
GCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGG	7	0.17500000000000002	No Hit
CTCAGGAGCTGGGGGTGGTGGTGGGGGCGGTGGGGGTGGTGTTAGTACCC	7	0.17500000000000002	No Hit
CTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCC	7	0.17500000000000002	No Hit
CCCCCATGGAGCACAGGCAGACAGAAGTCCCCACCCCAGCTGTGTGGCCT	7	0.17500000000000002	No Hit
GTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGC	7	0.17500000000000002	No Hit
CCCAGGCCTCCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTAT	7	0.17500000000000002	No Hit
GCCCCTACCCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTC	7	0.17500000000000002	No Hit
CAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCC	7	0.17500000000000002	No Hit
GCCTCTACAAGGATAATCTGACCTGCAGGGTCGAGGAGTTGACGGTGCTGAGTTCCCTGCACTCTCAGTAGGGAC	7	0.17500000000000002	No Hit
CTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGG	7	0.17500000000000002	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTT	7	0.17500000000000002	No Hit
CCCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTC	7	0.17500000000000002	No Hit
CCCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	7	0.17500000000000002	No Hit
CACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCA	7	0.17500000000000002	No Hit
CTCCATTCTAAGTGCTGGACATGGGGTGGCCATAATCTGGAGCTGATGGC	7	0.17500000000000002	No Hit
CCGGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAG	7	0.17500000000000002	No Hit
GCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGA	7	0.17500000000000002	No Hit
CTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTT	7	0.17500000000000002	No Hit
CCTCTGCCCTAGAAGTGAGAAGTCCAGAGCTCTACAGTTTGAAAACCACTATTTTATGAACCAAGTAGAACA	7	0.17500000000000002	No Hit
GGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCCAG	7	0.17500000000000002	No Hit
CCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	7	0.17500000000000002	No Hit
GTGGTGGTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCT	7	0.17500000000000002	No Hit
GTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAG	7	0.17500000000000002	No Hit
AACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCT	7	0.17500000000000002	No Hit
ACCCAGGCCTCCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTA	7	0.17500000000000002	No Hit
CTGGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCC	7	0.17500000000000002	No Hit
CTCGCCTCCCTCAATGGCCACTCCGTGCTCCAGCCATGCTGGCTTCCTTT	7	0.17500000000000002	No Hit
GTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCC	7	0.17500000000000002	No Hit
CAGGTTCTCAGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGT	7	0.17500000000000002	No Hit
CTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTC	7	0.17500000000000002	No Hit
CTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCT	7	0.17500000000000002	No Hit
CCCTGCCCTCCCTGCTTGCAAGTCCCCTGTCTGTAGCCTCACCCCTGTCG	6	0.15	No Hit
CTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAG	6	0.15	No Hit
CAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCAC	6	0.15	No Hit
GACTGATTCCAGCCTTGCACGGAGGGATCTGGCAGAGTCCATCCAGTTCA	6	0.15	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCA	6	0.15	No Hit
CTCGTGACGGCCAGCAGAGCCGGCCCCCATCTCCTCTGACCTCCCACCTC	6	0.15	No Hit
GCCTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAG	6	0.15	No Hit
GCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTC	6	0.15	No Hit
CTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCA	6	0.15	No Hit
ACAGCTGATAATTTATTAGTTAGGCTCATTTGTAATTTTTGTCACTATAGACCAGTTTTTTATTTAAATTGA	6	0.15	No Hit
ATCCAGAAGTCCCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTC	6	0.15	No Hit
ATGACAGACTGGTATGAAGGTGGCCACAATTCAGAAAGAAAAAAGAAGAG	6	0.15	No Hit
GTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTC	6	0.15	No Hit
CCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTG	6	0.15	No Hit
CAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGCCTTGGCCCCCAGA	6	0.15	No Hit
GTTGCTGTAGTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGA	6	0.15	No Hit
CCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTC	6	0.15	No Hit
CTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCA	6	0.15	No Hit
ACTCCGTGCTCCAGCCATGCTGGCTTCCTTTTAGGTCCTCCAACTCCAGGCTGTAGTTCATGTGCTTCTTTCTGG	6	0.15	No Hit
CCCACCGAAAAAGCCCATGGACAACGAAAAGCCCACTAGCTTGTCCAGTGCCACAGGAGGGGCAAGTGGAGGAGG	6	0.15	No Hit
CTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCTG	6	0.15	No Hit
GTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGA	6	0.15	No Hit
GATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGG	6	0.15	No Hit
CCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCC	6	0.15	No Hit
AGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGT	6	0.15	No Hit
CTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGG	6	0.15	No Hit
GGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAG	6	0.15	No Hit
CTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTG	6	0.15	No Hit
CTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGT	6	0.15	No Hit
GTCAGGCCGCCCCTACCCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGA	6	0.15	No Hit
GGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCCAAAGGGCACAGTTGAACAATGG	6	0.15	No Hit
CTCAATGGCCACTCCGTGCTCCAGCCATGCTGGCTTCCTTTTAGGTCCTC	5	0.125	No Hit
GGGCTGCAAAGTGAAGGAGCAGGGGCTCCAGGTCTGGCGACAACCAGGGA	5	0.125	No Hit
CTCCGTGCTCCAGCCATGCTGGCTTCCTTTTAGGTCCTCCACCTCCAGGCTGTAGTTCATGTGCTTCTTTCTGGA	5	0.125	No Hit
CAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGT	5	0.125	No Hit
GGGGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCT	5	0.125	No Hit
CACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTT	5	0.125	No Hit
CTACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGA	5	0.125	No Hit
GCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCA	5	0.125	No Hit
GTCCAGAGCTCTACAGTTTGAAAACCACTATTTTATGAACCAAGTAGAACAAGATATTTGAAATCGAAACTATTC	5	0.125	No Hit
GGGGCTCAGGCATGGCAGGGCTGGGAGTACCAGCAGGCACTCAAGCGGCT	5	0.125	No Hit
CTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGAGGAACCTTCCCC	5	0.125	No Hit
CCCCTTCTTTCCATTGGTTTAATTAGGAATGGGGAACCCAGAGCCTCACTTGTTCAGGCTCCCTCTGCCCTAGA	5	0.125	No Hit
GCCAGAGGGTAGACTGCAATCACCAAGATGAAATTTACAAGGAACAAATGTGAAGCCCAACATTTAGGTTTTAAA	5	0.125	No Hit
GGCCTTCTTGCTGCCCTTGATCTTCTCAATCTTGGCCTGGGCCAAGGAGA	5	0.125	No Hit
CCCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAA	5	0.125	No Hit
CAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTC	5	0.125	No Hit
GGGGACAAGAGGAAAGAGGTGCCTGTGGGGGTGGAGGACAGGAAGGAAAA	5	0.125	No Hit
CTCTACAGTTTGAAAACCACTATTTTATGAACCAAGTAGAACAAGATATT	5	0.125	No Hit
GCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCACCTGGCTCCGGCT	5	0.125	No Hit
GTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCG	5	0.125	No Hit
ATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGA	5	0.125	No Hit
CCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGC	5	0.125	No Hit
GCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGA	5	0.125	No Hit
CCAGTTTTGTGAAGTGCCTAGAATAGTCAAAATTCACAGAGACAGAAGCAGTGGTCGCCAGGAATGGGGAAGCA	5	0.125	No Hit
CCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGG	5	0.125	No Hit
ACCACAGAAAACAGAGGGTGACTGTCATCCCCTCCAGTCTCTGCACACTCCCAGCTGCAGCAGAGCAGGAGGAG	5	0.125	No Hit
CTCCAACTCCAGGCTGTAGTTCATGTGCTTCTTTCTGGAATGTTCTTCCC	5	0.125	No Hit
CGCACACACGAGCCAGCAGAGGGGTTTTGTGCCACTTCTGGATGCTAGGGTTAGACTGGGAGATACAGCAGTGA	5	0.125	No Hit
CCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCC	5	0.125	No Hit
GTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACC	5	0.125	No Hit
GTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGC	5	0.125	No Hit
GTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCC	5	0.125	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	5	0.125	No Hit
CCAGAGGGTAGACTGCAATCACCAAGATGAAATTTACAAGGAACAAATGTGAAGCCCAACATTTAGGTTTTAAAA	5	0.125	No Hit
CCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	5	0.125	No Hit
CGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGG	5	0.125	No Hit
GGGGAAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTC	5	0.125	No Hit
TGCCCCCACCCTGCAGAGCTGGACCCCTGAGCTAGCCATGCTCTGACAGTCTCAGTTGCACACACGAGCCAGC	5	0.125	No Hit
CTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGCAGGACC	5	0.125	No Hit
GGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAGGC	5	0.125	No Hit
CCTCAATGGCCACTCCGTGCTCCAGCCATGCTGGCTTCCTTTTAGGTCCT	5	0.125	No Hit
CCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGC	5	0.125	No Hit
TACAGTTTGAAAACCACTATTTTATGAACCAAGTAGAACAAGATATTTGAAATCGAAACTATTCAAAAAATTG	5	0.125	No Hit
CTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAG	5	0.125	No Hit
GGGGAATCTGTAGGGTCAATGGAGGAGTTCAGAGAAGGTGCAACATTTCT	5	0.125	No Hit
CTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGG	5	0.125	No Hit
GGGGTCCCAGGCCTCCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGG	5	0.125	No Hit
AGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGAC	5	0.125	No Hit
GGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCC	5	0.125	No Hit
CTCACTCCGAGCTCCCGACGTGCACACGGCTCCCATGCGTTGTCTTCCGA	5	0.125	No Hit
AGGGGAGTAGGGGACAGAGCATTTGCTGAGAGGCCAGGAGCGCATAGATGGGACTCTGCTGATGCCTGCTGAGTG	5	0.125	No Hit
CGGTGCTGAGTTCCCTGCACTCTCAGTAGGGACAGGCCCTATGCTGCCAC	5	0.125	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCT	5	0.125	No Hit
CTGCTTGCAAGTCCCCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTA	5	0.125	No Hit
CTCCAGCTTTCGCTCCTTCATGCTGCGCAGCTTGGCCTTGCCGATGCCCCCAGCTTGGCGGATGGACTCTAGC	5	0.125	No Hit
AGCCACTTCTGGATGCTAGGGTTACACTGGGAGACACAGCAGTGAAGCTG	5	0.125	No Hit
GCTCTGGGTCCCCACTAAGCCAGGCCGGGCCTCCCGCCCACACCCCTCGG	5	0.125	No Hit
GTCCCCATCTTGGCAAGGAAACACAATTTCCGAGGGAATGGTTTTGGCCT	5	0.125	No Hit
CCTCATTCTTAGTATGGTTTTTCACTTTAAAAAGCATAAAGGTGGACATGGTGGCATATGCTAGTAATCCCAACT	5	0.125	No Hit
CCCATCCCTGCCCTGCTCAAAATCCAATCACAGCTCCCTAACACGCCTGAATCAACTTGAAGTCCTGTCTTGAGT	5	0.125	No Hit
GTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACA	5	0.125	No Hit
GTCGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCC	5	0.125	No Hit
CTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAG	5	0.125	No Hit
GGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
ERR5303527 read2 length is 41-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5303527_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41-76
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0245	38.0	32.0	38.0	32.0	38.0
2	36.00575	38.0	32.0	38.0	32.0	38.0
3	35.8295	38.0	32.0	38.0	32.0	38.0
4	35.71125	38.0	32.0	38.0	32.0	38.0
5	35.84725	38.0	32.0	38.0	32.0	38.0
6	38.36375	40.0	38.0	40.0	38.0	40.0
7	38.446	40.0	38.0	40.0	38.0	40.0
8	38.36125	40.0	38.0	40.0	38.0	40.0
9	38.431	40.0	38.0	40.0	38.0	40.0
10-11	38.377250000000004	40.0	38.0	40.0	38.0	40.0
12-13	38.4705	40.0	38.0	40.0	38.0	40.0
14-15	38.438	40.0	38.0	40.0	38.0	40.0
16-17	38.401624999999996	40.0	38.0	40.0	38.0	40.0
18-19	38.37075	40.0	38.0	40.0	38.0	40.0
20-21	38.376625	40.0	38.0	40.0	38.0	40.0
22-23	38.36725	40.0	38.0	40.0	38.0	40.0
24-25	38.356875	40.0	38.0	40.0	38.0	40.0
26-27	38.360125	40.0	38.0	40.0	38.0	40.0
28-29	38.388000000000005	40.0	38.0	40.0	38.0	40.0
30-31	38.3505	40.0	38.0	40.0	38.0	40.0
32-33	38.422	40.0	38.0	40.0	38.0	40.0
34-35	38.26625	40.0	38.0	40.0	38.0	40.0
36-37	38.30275	40.0	38.0	40.0	38.0	40.0
38-39	38.286375	40.0	38.0	40.0	38.0	40.0
40-41	38.32025	40.0	38.0	40.0	38.0	40.0
42-43	38.255188797199295	40.0	38.0	40.0	38.0	40.0
44-45	38.27431857964491	40.0	38.0	40.0	38.0	40.0
46-47	38.268192048012	40.0	38.0	40.0	38.0	40.0
48-49	38.258124763865474	40.0	38.0	40.0	38.0	40.0
50-51	38.173755316487366	40.0	38.0	40.0	38.0	40.0
52-53	38.16103603603604	40.0	38.0	40.0	38.0	40.0
54-55	38.20868803204807	40.0	38.0	40.0	38.0	40.0
56-57	38.276289434151224	40.0	38.0	40.0	38.0	40.0
58-59	38.13357536304457	40.0	38.0	40.0	38.0	40.0
60-61	38.0578367551327	40.0	38.0	40.0	38.0	40.0
62-63	38.07598898347521	40.0	38.0	40.0	38.0	40.0
64-65	38.12183821687954	40.0	38.0	40.0	38.0	40.0
66-67	38.056784825521945	39.0	38.0	40.0	38.0	40.0
68-69	38.064267996143286	38.0	38.0	40.0	38.0	40.0
70-71	38.07627747711791	38.0	38.0	40.0	38.0	40.0
72-73	38.079047202713525	38.0	38.0	40.0	38.0	40.0
74-75	38.27277047957746	40.0	38.0	40.0	38.0	40.0
76	37.881910492666414	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	1.0
21	0.0
22	4.0
23	6.0
24	9.0
25	6.0
26	15.0
27	11.0
28	20.0
29	28.0
30	19.0
31	34.0
32	32.0
33	63.0
34	57.0
35	106.0
36	163.0
37	364.0
38	1228.0
39	1832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.28357089272318	16.954238559639908	7.726931732933233	41.035258814703674
2	31.45	21.075	23.425	24.05
3	27.200000000000003	22.25	22.025	28.525
4	29.849999999999998	26.650000000000002	18.875	24.625
5	37.3	24.474999999999998	17.125	21.099999999999998
6	30.45	29.375	16.45	23.724999999999998
7	27.425	18.8	26.5	27.275
8	26.525	20.0	20.599999999999998	32.875
9	25.75	20.5	24.8	28.95
10-11	30.825000000000003	24.5125	18.925	25.7375
12-13	30.125	18.4375	21.337500000000002	30.099999999999998
14-15	30.025000000000002	22.237499999999997	21.425	26.3125
16-17	34.0375	20.2375	19.75	25.974999999999998
18-19	30.625000000000004	20.175	19.825	29.375
20-21	30.912499999999998	20.9375	19.3625	28.787499999999998
22-23	31.95	20.4125	20.775	26.8625
24-25	31.2625	20.625	19.35	28.762500000000003
26-27	29.32866608326041	19.889986248281037	20.840105013126642	29.94124265533192
28-29	31.025000000000002	19.8	20.6125	28.5625
30-31	30.666333291661456	19.71496437054632	20.665083135391924	28.9536192024003
32-33	29.47868483560445	20.577572196524567	20.75259407425928	29.1911488936117
34-35	30.837500000000002	21.987499999999997	18.875	28.299999999999997
36-37	28.710766537451544	19.569838689508565	20.50769038389396	31.21170438914593
38-39	30.827913956978488	20.060030015007506	19.522261130565283	29.589794897448723
40-41	31.17029257314329	19.72993248312078	20.7551887971993	28.34458614653663
42-43	29.756097560975608	19.537210756722953	19.924953095684803	30.781738586616637
44-45	31.103051525762883	20.58529264632316	21.848424212106053	26.463231615807903
46-47	30.673004753565174	21.240930698023515	19.06429822366775	29.02176632474356
48-49	30.897259416843948	18.57089225378551	20.535602552871982	29.996245776498558
50-51	29.227688071097756	20.403054199524345	21.517085993240705	28.852171736137187
52-53	30.633450175262894	20.080120180270406	19.86730095142714	29.41912869303956
54-55	27.90581162324649	21.9313627254509	19.626753507014026	30.53607214428858
56-57	27.664370695053226	22.15403882279274	20.626174076393237	29.555416405760802
58-59	29.145791583166332	21.16733466933868	19.777054108216433	29.909819639278556
60-61	28.954289292423297	20.012523481527865	21.252348152786475	29.780839073262367
62-63	28.265497808390734	22.241703193487787	20.801502817783344	28.69129618033813
64-65	30.172845691382765	21.417835671342687	19.852204408817638	28.557114228456914
66-67	33.40438871473354	19.222570532915363	20.63949843260188	26.733542319749215
68-69	30.980786135878436	22.303152078362427	19.465025744066306	27.251036041692828
70-71	30.387713997985898	20.73262839879154	18.94511581067472	29.934541792547837
72-73	30.015159171298635	20.212228398180898	21.235472460838807	28.537139969681657
74-75	33.49814585908529	17.41519022112347	21.054800164812526	28.031863754978716
76	33.73448664911621	0.0	28.61978187288454	37.64573147799925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	0.0
18	0.0
19	0.0
20	0.0
21	3.0
22	3.5
23	1.5
24	4.0
25	9.0
26	13.0
27	13.0
28	12.0
29	13.0
30	9.5
31	4.0
32	5.0
33	8.0
34	9.5
35	12.0
36	17.5
37	17.0
38	16.0
39	34.5
40	51.0
41	40.0
42	32.0
43	37.5
44	39.0
45	54.0
46	66.5
47	62.0
48	55.0
49	84.0
50	108.5
51	114.5
52	124.5
53	162.5
54	216.5
55	229.5
56	274.0
57	254.0
58	189.5
59	222.5
60	244.5
61	212.0
62	186.5
63	184.0
64	215.5
65	215.5
66	203.0
67	228.0
68	224.0
69	151.5
70	99.0
71	109.0
72	116.5
73	86.0
74	38.5
75	17.0
76	6.5
77	8.5
78	12.5
79	14.5
80	14.5
81	8.0
82	6.0
83	7.5
84	9.0
85	15.5
86	14.0
87	4.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0125
28-29	0.0
30-31	0.0125
32-33	0.0125
34-35	0.0
36-37	0.0375
38-39	0.05
40-41	0.025
42-43	0.037509377344336084
44-45	0.025006251562890724
46-47	0.05001250312578145
48-49	0.06253126563281641
50-51	0.06254691018263697
52-53	0.050050050050050046
54-55	0.050075112669003496
56-57	0.03755633450175263
58-59	0.050075112669003496
60-61	0.03755633450175263
62-63	0.03755633450175263
64-65	0.025043826696719257
66-67	0.025072082236429732
68-69	0.03766005523474768
70-71	0.025169896803423106
72-73	0.012631047113805733
74-75	0.013732491073880802
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
41	1.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	2.0
49	0.0
50	0.0
51	1.0
52	0.0
53	2.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	1.0
64	0.0
65	4.0
66	1.0
67	4.0
68	2.0
69	7.0
70	4.0
71	4.0
72	17.0
73	103.0
74	412.0
75	776.0
76	2659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.98094027954257	17.7
2	21.28335451080051	16.75
3	14.23125794155019	16.8
4	6.861499364675986	10.8
5	4.701397712833545	9.25
6	2.4777636594663277	5.8500000000000005
7	1.3977128335451081	3.85
8	1.0165184243964422	3.2
9	0.6353240152477764	2.25
>10	2.41423125794155	13.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTC	35	0.8750000000000001	No Hit
GCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	20	0.5	No Hit
CCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGG	19	0.475	No Hit
CAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGA	19	0.475	No Hit
CTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTG	19	0.475	No Hit
CCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCAT	18	0.44999999999999996	No Hit
GTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTC	17	0.42500000000000004	No Hit
GGGACAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCC	16	0.4	No Hit
CTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAG	16	0.4	No Hit
CAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAG	16	0.4	No Hit
CCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCT	16	0.4	No Hit
CCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACA	16	0.4	No Hit
TGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCA	16	0.4	No Hit
GGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAG	16	0.4	No Hit
CGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTT	15	0.375	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	15	0.375	No Hit
AGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAG	14	0.35000000000000003	No Hit
CGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTG	13	0.325	No Hit
CTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACC	13	0.325	No Hit
GGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGC	13	0.325	No Hit
CCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCA	13	0.325	No Hit
CAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCA	13	0.325	No Hit
GGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACC	13	0.325	No Hit
CGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCT	12	0.3	No Hit
CGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCAC	12	0.3	No Hit
GGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCC	11	0.27499999999999997	No Hit
CTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTGTGCCCTTTGGGGC	11	0.27499999999999997	No Hit
CCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAG	11	0.27499999999999997	No Hit
GTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGC	11	0.27499999999999997	No Hit
TGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTG	11	0.27499999999999997	No Hit
CTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGAC	11	0.27499999999999997	No Hit
GGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCA	11	0.27499999999999997	No Hit
CCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGA	10	0.25	No Hit
CTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGA	10	0.25	No Hit
CCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCT	10	0.25	No Hit
ACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	10	0.25	No Hit
AAATGACATTTATGGTAAAGTCTGAGGGTTGAGTGGGTAGGTTGGGAAGA	10	0.25	No Hit
GGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCT	10	0.25	No Hit
GGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGT	9	0.22499999999999998	No Hit
CTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCTCCATG	9	0.22499999999999998	No Hit
CTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGGACT	9	0.22499999999999998	No Hit
TGACATTTATGGTAAAGTCTGAGGGTTGAGTGGGTAGGTTGGGAAGAACATTCCAGAAAGAAGCACATGAACTAC	9	0.22499999999999998	No Hit
ATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGC	9	0.22499999999999998	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	9	0.22499999999999998	No Hit
CCTGAGTCCACCGGGTGCTTTCTGCCCACCCCCTGCTCTTGCCAGCTGGC	9	0.22499999999999998	No Hit
CGCAGGAAAGCGGGTCAAGGCGTAGGGCTGGAGGGCAGGGGCGGGCCCTG	9	0.22499999999999998	No Hit
AATGAACTGGATGGACTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAG	9	0.22499999999999998	No Hit
GCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCA	9	0.22499999999999998	No Hit
CCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGGACACCCAGAAGCTGTTATTGT	8	0.2	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	8	0.2	No Hit
CAGTCTGTCATGGAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATGCCTGAGCCCCCTGC	8	0.2	No Hit
GGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGA	8	0.2	No Hit
CGGGTGGCTCGGCTCGGGAGGCCTGGGACCCCACAGTGCACGCTGTGCCC	8	0.2	No Hit
GGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGGACACCCAGAAGCT	8	0.2	No Hit
GCCTGACCTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGG	8	0.2	No Hit
CGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAAC	8	0.2	No Hit
CCTCACTCAGCCTTTTCCCTCCAGGCATCTCTGGGAAAGGACCTGGGGCT	8	0.2	No Hit
CTTTAGGGCACGGGGACTTCTGGATTGTGAAATTGGCTCTCTGGGGGCCA	8	0.2	No Hit
CCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTG	8	0.2	No Hit
GGCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGC	8	0.2	No Hit
CATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACCATTCCAGAACTGCCCACCTTCCACACTG	8	0.2	No Hit
GGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGC	8	0.2	No Hit
CCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGG	8	0.2	No Hit
CTTCAACACCACCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCC	8	0.2	No Hit
GCCTGAGCCCCCTGCACACAAGGAGCCAGGAGTAATCAGGGCAGACCCTT	7	0.17500000000000002	No Hit
AGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCT	7	0.17500000000000002	No Hit
CCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATCTTCACGGGCGCCC	7	0.17500000000000002	No Hit
GTGAGTTAGGGCCCACGGATTACTCAAGACAGGACTTCAAGTTGATTCAG	7	0.17500000000000002	No Hit
CCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTACATTGCC	7	0.17500000000000002	No Hit
CATGGAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATGCCTGAGCCCCCTGCACACAAGG	7	0.17500000000000002	No Hit
GGATGAGTGAGTTAGGGCCCACGGATTACTCAAGACAGGACTTCAAGTTG	7	0.17500000000000002	No Hit
GGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTG	7	0.17500000000000002	No Hit
CAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAG	7	0.17500000000000002	No Hit
GCGGGAGGCCCGGCCTGGCTTAGTGGGGACCCAGGGCATCAGACACAGGT	7	0.17500000000000002	No Hit
CGCCTGCAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCT	7	0.17500000000000002	No Hit
GGGGATGACAGTCACCCTCTGTTTTCTGTGGTGGCTCTGTTTTCTGTGGT	7	0.17500000000000002	No Hit
GCTGTGATTGGATTTTGAGCAGGGCAGGGATGGGACAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGG	7	0.17500000000000002	No Hit
TGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTC	7	0.17500000000000002	No Hit
GAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCT	7	0.17500000000000002	No Hit
CACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGA	7	0.17500000000000002	No Hit
TGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCG	7	0.17500000000000002	No Hit
AGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGC	7	0.17500000000000002	No Hit
GTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGG	7	0.17500000000000002	No Hit
CAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCCAGTGCCAAGTACCCTG	7	0.17500000000000002	No Hit
AGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGC	7	0.17500000000000002	No Hit
GGCACGGGGACTTCTGGATTGTGAAATTGGCTCTCTGGGGGCCAAGGCCT	7	0.17500000000000002	No Hit
GAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAAC	6	0.15	No Hit
GTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCT	6	0.15	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGC	6	0.15	No Hit
CTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCC	6	0.15	No Hit
AGCAGATGGCGGATGCCCTGCAGTACCTGCAGAAGGTCTCTGGAGACATC	6	0.15	No Hit
CTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGA	6	0.15	No Hit
ATGTGGGTCCCAAGGTGGGACCACATCAGACATGTTCGAGGACTAGCTGAGGGAGAGAGGTGGGAGGTCAGAGG	6	0.15	No Hit
GGAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATG	6	0.15	No Hit
CACTTCACAAAACTGGACTCATACGATATCTGTAGTTTTTCTCTATTGAA	6	0.15	No Hit
GACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	6	0.15	No Hit
GATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGC	6	0.15	No Hit
AAAATAGTGGTTTTCAAACTGTAGAGCTCTGGACTTCTCACTTCTAGGGC	6	0.15	No Hit
CTCTGGACTTCTCACTTCTAGGGCAGAGGGAGCCTGAACAAGTGAGGCTCTGGGTTCCCCATTCCTAATTAAACC	6	0.15	No Hit
ATCATGAAACCAGCTTACCCTTCCCCTGTGTGCTGGCCCCAGTTTTCTAA	6	0.15	No Hit
CCGAGAACCTGTATGGCCAGAGGGCAGGGCCGAGGGGTGTGGGCGGGAGGCCCGGCCTGGCTTAGTGGGGACCC	6	0.15	No Hit
CCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGC	6	0.15	No Hit
GGATTACTCAAGACAGGACTTCAAGTTGATTCAGGCGTGTTAGGGAGCTGTGATTGGATTTTGAGCAGGGCAGGG	6	0.15	No Hit
CAGGCCTCTTTGAATGGGGCTGAGGGAGCTTTGCCCTGGTTCCTTTGTGT	6	0.15	No Hit
CTTCATACCAGTCTGTCATGGAACACTTAAGCCGCTTGAGTGCCTGCTGG	6	0.15	No Hit
CACCTTCATACCAGTCTGTCATGGAACACTTAAGCCGCTTGAGTGCCTGC	6	0.15	No Hit
CGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	6	0.15	No Hit
CTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGA	6	0.15	No Hit
CCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAG	6	0.15	No Hit
CTCCTGGCAAATTAGCATTGCAGGCTGTGCTCTCTCCTCCTGCTCTGCTGCAGCTGGGAGTGTGCAGAGACTGG	6	0.15	No Hit
CCTCTGGCCTTTAAAAATTGTCTGAGCCTTGATTCGATCATGAAACCAGC	6	0.15	No Hit
GGAATCAGTCCATTGTTCAACTGTGCCCTTTGGGGCTGTGGTTCATTTGG	6	0.15	No Hit
AACTGAGACAAGGTATGGAAAGGGTGCCTGGCAGGTAGCAAGCACCTTGT	6	0.15	No Hit
ACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGG	6	0.15	No Hit
CTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCT	6	0.15	No Hit
CGGGGACTTCTGTCTGCCTGTGCTCCATGGGGGGACGGCTCCACCCAGCC	6	0.15	No Hit
GGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGACACC	6	0.15	No Hit
GGGGGTCCTGCCCCGGGGCGCACCCCGGGCGAGGGCTGCCCGGAGGAGCCGAGGTTGGCGGACAGCTTGGCCCTG	6	0.15	No Hit
TGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGG	6	0.15	No Hit
GTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTA	6	0.15	No Hit
ATTTATGGTAAAGTCTGAGGGTTGAGTGGGTAGGTTGGGAAGAACATTCC	6	0.15	No Hit
ACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATGCCTGAGCCCCCTGCACACAAGGAGCCAGG	6	0.15	No Hit
GCCAGGAGTAATCAGGGCAGACCCTTTAGGGCACGGGGACTTCTGGATTG	6	0.15	No Hit
GGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTC	6	0.15	No Hit
GGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCT	6	0.15	No Hit
AGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGCAGCTGGAACAG	5	0.125	No Hit
ACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGT	5	0.125	No Hit
GCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCT	5	0.125	No Hit
CTCTGCTAGAGTCCATCCGCCAAGCTGGGGGCATCGGCAAGGCCAAGCTGCGCAGCATGAAGGAGCGAAAGCTGG	5	0.125	No Hit
GCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTATGGC	5	0.125	No Hit
ATAGTAACAAAGTGCTGGATGCACCTTTTGTGCTTATCTTTGTGCTAAATGTGCCCGAGGGACACCTAGGGAAG	5	0.125	No Hit
CATGCTTTTAGCCCCTACTGAGCTTATAGTTAACAGAAGTTTCCAGGTCT	5	0.125	No Hit
CCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTG	5	0.125	No Hit
CCCAGAAGCTGTTATTGTAGTCAGGATACGACAGGGGTGAGGCTACAGACAGGGGACTTGCAAGCAGGGAGGGC	5	0.125	No Hit
CTGTAGGCCAAGGCGCCAGGCAGGACGACAGCAGCAGCAGCGCGTCTCCT	5	0.125	No Hit
ATGTGTAAATACCATCCTCTGTGTGCCCTGGAGGCTGTCCTTCAGATAGC	5	0.125	No Hit
GTTGAATGAACTGGATGGACTCTGCCAGATCCCTCCGTGCAAGGCTGGAA	5	0.125	No Hit
CAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCA	5	0.125	No Hit
CTCAGGTCCAGCCTTGCCAGTCTCAGCCTCCGGTCTCCGTTCCCCCTCTG	5	0.125	No Hit
GGACGACTGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCC	5	0.125	No Hit
GGGGCGGGCCCTGGGCGTGGGCTGGGGGTCCTGCCCCGGGGCGCACCCCGGGCGAGGGCTGCCCGGAGGAGCCG	5	0.125	No Hit
CAGCGATGGGACAAAGGACGGAGGTCTAGGAAGAGGGTCTGCAGAGCAGA	5	0.125	No Hit
GTTGGGATGAGTGAGTTAGGGCCCACGGATTACTCAAGACAGGACTTCAA	5	0.125	No Hit
GGACAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCC	5	0.125	No Hit
CCAAGAGAGTGCACGGTCCCTGCTGATTGAAAGAAGGATGAAGGGCAGAAGAGGGGCGGGGAGCTGTGTGCCCTA	5	0.125	No Hit
CGGGTGCTTTCTGCCCACCCCCTGCTCTTGCCAGCTGGCCCCTGCTTCCC	5	0.125	No Hit
CCTTCCCAGGATCCCACACCATGCCTCACATGGTAGACCCCAGTGGGAAG	5	0.125	No Hit
CTTGGGCAAGATCGCCTCCTCCCCTCCAGCCCTGAGTCCACCGGGTGCTT	5	0.125	No Hit
TCCCAGTGTAACCCTAGCATCCAGAAGTGGCACAAAACGCCTCTGCTGGCTCGTGTGTGCAACTGAGACTGTC	5	0.125	No Hit
CCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTC	5	0.125	No Hit
ATTTATCAAGTATATTTCCCTTGTTTTTTCCTAGTATATTAATTTATTTACTTATCTTCTAAAAATCCTCCATAT	5	0.125	No Hit
GAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTC	5	0.125	No Hit
GAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTC	5	0.125	No Hit
GCCATCCCTGTCCTGGGCAATCCCAGCAACACACCCTCTGGGAGCAGCCCCCTTGGGGAATCCCGGTCCTGGGGA	5	0.125	No Hit
AGTTAATCTGCTGTAGCTCAAGAGAGGATGTACCTGCCCCCACCCCGCATCCCCGGGCTCGGGTTTGCCTTGCTG	5	0.125	No Hit
CTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAG	5	0.125	No Hit
ACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGAC	5	0.125	No Hit
CCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGA	5	0.125	No Hit
GACCTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGC	5	0.125	No Hit
CAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCC	5	0.125	No Hit
AATGACCTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACCATTCCAGAACTGCCC	5	0.125	No Hit
CCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAG	5	0.125	No Hit
CTCAATTTTTTGAATAGTTTCCATTTCAAATATCTTGTTCTACTTGGTTCATAAAATAGTGGTTTTCAAACTGT	5	0.125	No Hit
TGCCTGGCAGGTAGCAAGCACCTTGTAGGGGGTCAGAAATGTTGCACCTTCTCTGAACTCCTCCATTGACCCTAC	5	0.125	No Hit
CCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCC	5	0.125	No Hit
CCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGG	5	0.125	No Hit
CAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGG	5	0.125	No Hit
TGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCTCCATGGGGGGACGGCTCCACCCAGCCTGCGCC	5	0.125	No Hit
CGAGAGGAGGCCGTCCAGCAGATGGCGGATGCCCTGCAGTACCTGCAGAA	5	0.125	No Hit
TGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCG	5	0.125	No Hit
GGGAGAGAGAGTGGATGCCAGGGACACCCAGAAGCTGTTATTGTAGTCAG	5	0.125	No Hit
GAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	5	0.125	No Hit
GGCTGGAGGGCAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCCTGCCCCG	5	0.125	No Hit
AGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGC	5	0.125	No Hit
ATTAGCATTGCAGGCTGTGCTCTCTCCTCCTGCTCTGCTGCAGCTGGGAGTGTGCAGAGACTGGAGGGGATGAC	5	0.125	No Hit
GGTCTTTCTTCACCTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTG	5	0.125	No Hit
GGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAG	5	0.125	No Hit
CACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGG	5	0.125	No Hit
CTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTGTG	5	0.125	No Hit
AGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTG	5	0.125	No Hit
ACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGG	5	0.125	No Hit
GCTTCACATTTGTTCCTTGTAAATTTCATCTTGGTGATTGCAGTCTACCC	5	0.125	No Hit
ACTGGATGGACTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCCAT	5	0.125	No Hit
GCAGCGCGTCTCCTTCAGGTGGGAGCAGCTCTTTGAGGCCACCTGATTTCTGGCGTGCTCAGTGCACTCGGGTGG	5	0.125	No Hit
TTTCATCTTGGTGATTGCAGTCTACCCTCTGGCCTTTAAAAATTGTCTGA	5	0.125	No Hit
CAGCTCTTTGAGGCCACCTGATTTCTGGCGTGCTCAGTGCACTCGGGTGG	5	0.125	No Hit
GCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGC	5	0.125	No Hit
TGGTCTGCCGCGAAGGCCGGCCTGCACAGGGGTGGGAGGGGGGTGCTTCT	5	0.125	No Hit
GAACTGGATGGACTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCC	5	0.125	No Hit
GTGATTGCTGCTTTAGTGGCACCAGGGAGAGGAGTTAATTAAACCCAATATAAATAGACTCTGCCCTCACTTTGC	5	0.125	No Hit
AGAAGGCCATCAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGC	5	0.125	No Hit
GGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGC	5	0.125	No Hit
AGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCCAGT	5	0.125	No Hit
GTCTTTCTTCACCTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGT	5	0.125	No Hit
AGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTA	5	0.125	No Hit
CAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCCTGCCCCGGGGCGCACCC	5	0.125	No Hit
GTGGATGCCCTGCAGTACCTGCAGAAGGTCTCTGGAGACATCTTCAGCAGGTAGAGCAGAGCCGGAGCCAGGTGC	5	0.125	No Hit
GCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCA	5	0.125	No Hit
CGGGCGAGGGCTGCCCGGAGGAGCCGAGGTTGGCGGACAGCTTGGCCCTGAGCTTGAGGGGAAGGCAGCGATGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
Read 3106180 spots for ERR5303527.sra
Written 3106180 spots for ERR5303527.sra
2025-05-07T13:22:52 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open KI270467.1 as a RefSeq or as a WGS
Rejected 3555 READS because READLEN < 1
Read 3106195 spots for ERR5303527.sra
Written 3106195 spots for ERR5303527.sra
SRR ids: ['ERR5303527.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_vpav64ak
ERR5303527.sra spots: 49698895
blocks: [[1, 3106180], [3106181, 6212360], [6212361, 9318540], [9318541, 12424720], [12424721, 15530900], [15530901, 18637080], [18637081, 21743260], [21743261, 24849440], [24849441, 27955620], [27955621, 31061800], [31061801, 34167980], [34167981, 37274160], [37274161, 40380340], [40380341, 43486520], [43486521, 46592700], [46592701, 49698895]]
ERR5303527 file size 9437928
ERR5303527 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 16 -o ERR5303527 ERR5303527_1.fastq ERR5303527_2.fastq
Input file:	ERR5303527_1.fastq
Paired file:	ERR5303527_2.fastq
trimmed:	ERR5303527-trimmed-pair1.fastq, ERR5303527-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Wed May  7 13:29:21 2025 >> started

Wed May  7 13:30:25 2025 >> done (63.847s)
49696278 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
49696278 (100.00%) read pairs available; of these:
    2039 ( 0.00%) trimmed read pairs available after processing
49694239 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 25	      36	  0.00%
 26	      38	  0.00%
 27	      52	  0.00%
 28	      95	  0.00%
 29	      94	  0.00%
 30	     136	  0.00%
 31	     139	  0.00%
 32	     211	  0.00%
 33	     225	  0.00%
 34	     249	  0.00%
 35	     254	  0.00%
 36	     315	  0.00%
 37	     417	  0.00%
 38	     412	  0.00%
 39	     515	  0.00%
 40	     578	  0.00%
 41	     719	  0.00%
 42	     848	  0.00%
 43	     947	  0.00%
 44	     967	  0.00%
 45	    1050	  0.00%
 46	    1264	  0.00%
 47	    1391	  0.00%
 48	    1805	  0.00%
 49	    2119	  0.00%
 50	    3076	  0.01%
 51	    4202	  0.01%
 52	    4378	  0.01%
 53	    4587	  0.01%
 54	    5252	  0.01%
 55	    5608	  0.01%
 56	    5771	  0.01%
 57	    6442	  0.01%
 58	    7249	  0.01%
 59	    8079	  0.02%
 60	    9693	  0.02%
 61	   11242	  0.02%
 62	   12564	  0.03%
 63	   14251	  0.03%
 64	   16428	  0.03%
 65	   18790	  0.04%
 66	   20409	  0.04%
 67	   22845	  0.05%
 68	   26498	  0.05%
 69	   32230	  0.06%
 70	   42556	  0.09%
 71	   59348	  0.12%
 72	  108025	  0.22%
 73	  536945	  1.08%
 74	 4380976	  8.82%
 75	23386151	 47.06%
 76	20927807	 42.11%
49696278 reads passed initial QC


criterion=sequence-density
sequence-density=0.24
sequence-density-rank=1
fanout-score=13.18
fanout-score-rank=11
prefix-density=0.56
prefix-fanout=5.7
sequence=TTCCTCCTCTTC


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=20
fanout-score=64.76
fanout-score-rank=1
prefix-density=0.54
prefix-fanout=8.0
sequence=CCTCCTCCTCCTGCTCCTGCCGGGAATACACAACAGGAGGCACCTCGAGGACCTTGTCCACATTCTTCA


criterion=sequence-density
sequence-density=0.19
sequence-density-rank=1
fanout-score=9.63
fanout-score-rank=16
prefix-density=0.32
prefix-fanout=5.5
sequence=GAAGAGGAGGAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=71.54
fanout-score-rank=1
prefix-density=0.19
prefix-fanout=2.1
sequence=CAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGCAAGAGAAAGCCCTACCACAAGAAGCGGAAGTATGAGTTGGGGCGCCCAGCTGCCAACACCAAGATTGGCCCCCGCCGCATCCACACAGTCCGTGTGCGGGGAGGTAACAAGAAATACCGTGCCCTGAGGTTGGACGTGGGGAATTTCTCCTGGGGCTCAGAGTGTTGTACTCGTAAAACAAGGATCATCGATGTTGTCTACAATGCATCTAATAACGAGCTGGTTCGTACCAAGACCCTGGTGAAGAATTGCATCGTGCTCATCGACAGCACACCGTACCGACAGTGGTACGAGTCCCACTATGCGCTGCCCCTGGGCCGCAAGAAGGGAGCCAAGCTGACTCCTGAGGAAGAAGAGATTTTAAACAAAAAACGATCTAAAAAAATTCAGAAG
ERR5303527 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 07 13:30:53
                             Started mapping on |	May 07 13:30:54
                                    Finished on |	May 07 13:32:30
       Mapping speed, Million of reads per hour |	1863.61

                          Number of input reads |	49696278
                      Average input read length |	150
                                    UNIQUE READS:
                   Uniquely mapped reads number |	47713232
                        Uniquely mapped reads % |	96.01%
                          Average mapped length |	150.48
                       Number of splices: Total |	23051813
            Number of splices: Annotated (sjdb) |	22795318
                       Number of splices: GT/AG |	22838595
                       Number of splices: GC/AG |	163779
                       Number of splices: AT/AC |	23852
               Number of splices: Non-canonical |	25587
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.56
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.39
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1981580
             % of reads mapped to multiple loci |	3.99%
        Number of reads mapped to too many loci |	6
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.00%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1466	1466	1466
N_multimapping	1981580	1981580	1981580
N_noFeature	3511418	45188338	3919205
N_ambiguous	3791228	69842	1738297
UnstrandedReadsAssigned:40410586 PositiveStrandReadsAssigned:2455052 NegativeStrandReadsAssigned:42055730
Dataset is classified negative stranded
MeadianReadLen=76 20thPercentileLength=75 echo kmer=71
ERR5303527 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in paired-end mode
[quant] will process pair 1: ERR5303527-trimmed-pair1.fastq
                             ERR5303527-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 49,696,278 reads, 43,841,554 reads pseudoaligned
[quant] estimated average fragment length: 150.091
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,411 rounds

 180870 ERR5303527.ke.tsv
  58302 ERR5303527.se.tsv
 239172 total
==> ERR5303527.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3	0	0
ENST00000448914.1	13	7	0	0
ENST00000415118.1	8	2	0	0
ENST00000631435.1	12	6	0	0
ENST00000632684.1	12	6	0	0
ENST00000454908.1	17	7.66667	0	0
ENST00000390567.1	20	10.6667	0	0
ENST00000603326.1	19	9.66667	0	0
ENST00000414852.1	16	10	0	0

==> ERR5303527.se.tsv <==
ENSG00000223972	0
ENSG00000227232	86
ENSG00000278267	18
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	7
ERR5303527 completed mapping pipeline successfully
