Starting /dee2/code/volunteer_pipeline.sh ERR5305084
    current disk space = 1435393593344
    free memory = 73880228 
ERR5305084 SRAfilesize
eada8a2a6b2addecab4921645074b38b  ERR5305084.sra
ERR5305084.sra file validated
ERR5305084 is paired end
ERR5305084 is conventional basespace
ERR5305084 read1 length is 53-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5305084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	53-76
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.89275	38.0	38.0	38.0	32.0	38.0
2	36.73375	38.0	38.0	38.0	32.0	38.0
3	36.72025	38.0	38.0	38.0	32.0	38.0
4	36.6955	38.0	38.0	38.0	32.0	38.0
5	36.61475	38.0	38.0	38.0	32.0	38.0
6	37.47875	38.0	38.0	40.0	32.0	40.0
7	37.99075	38.0	38.0	40.0	38.0	40.0
8	38.30975	38.0	38.0	40.0	38.0	40.0
9	38.46375	40.0	38.0	40.0	38.0	40.0
10-11	38.53125	40.0	38.0	40.0	38.0	40.0
12-13	38.527625	40.0	38.0	40.0	38.0	40.0
14-15	38.48325	40.0	38.0	40.0	38.0	40.0
16-17	38.509375	40.0	38.0	40.0	38.0	40.0
18-19	38.467875	40.0	38.0	40.0	38.0	40.0
20-21	38.460625	40.0	38.0	40.0	38.0	40.0
22-23	38.451375	40.0	38.0	40.0	38.0	40.0
24-25	38.46875	40.0	38.0	40.0	38.0	40.0
26-27	38.39775	40.0	38.0	40.0	38.0	40.0
28-29	38.415625	40.0	38.0	40.0	38.0	40.0
30-31	38.398625	40.0	38.0	40.0	38.0	40.0
32-33	38.440375	40.0	38.0	40.0	38.0	40.0
34-35	38.42375	40.0	38.0	40.0	38.0	40.0
36-37	38.348375000000004	40.0	38.0	40.0	38.0	40.0
38-39	38.403375	40.0	38.0	40.0	38.0	40.0
40-41	38.371875	40.0	38.0	40.0	38.0	40.0
42-43	38.37775	40.0	38.0	40.0	38.0	40.0
44-45	38.37587499999999	40.0	38.0	40.0	38.0	40.0
46-47	38.325125	40.0	38.0	40.0	38.0	40.0
48-49	38.35425	40.0	38.0	40.0	38.0	40.0
50-51	38.306125	40.0	38.0	40.0	38.0	40.0
52-53	38.3495	40.0	38.0	40.0	38.0	40.0
54-55	38.31357839459865	40.0	38.0	40.0	38.0	40.0
56-57	38.24868717179295	40.0	38.0	40.0	38.0	40.0
58-59	38.292356981191276	40.0	38.0	40.0	38.0	40.0
60-61	38.25444083062297	40.0	38.0	40.0	38.0	40.0
62-63	38.181931931931935	40.0	38.0	40.0	38.0	40.0
64-65	38.16904404404404	40.0	38.0	40.0	38.0	40.0
66-67	38.13629536921151	40.0	38.0	40.0	38.0	40.0
68-69	38.088520273432195	40.0	38.0	40.0	38.0	40.0
70-71	38.14257373223534	40.0	38.0	40.0	38.0	40.0
72-73	38.106699598938484	40.0	38.0	40.0	38.0	40.0
74-75	38.28026757611659	40.0	38.0	40.0	38.0	40.0
76	38.120208410867136	38.0	38.0	40.0	38.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	5.0
26	7.0
27	7.0
28	19.0
29	25.0
30	32.0
31	33.0
32	50.0
33	62.0
34	80.0
35	120.0
36	169.0
37	351.0
38	930.0
39	2109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.314314314314316	7.232232232232231	5.355355355355355	48.0980980980981
2	21.975	9.0	34.8	34.225
3	22.075	10.174999999999999	22.25	45.5
4	27.224999999999998	18.0	20.275000000000002	34.5
5	30.49574361542313	20.906359539308962	24.73710565848773	23.86079118678017
6	30.275000000000002	21.325	21.55	26.85
7	26.3	18.6	32.7	22.400000000000002
8	25.6	18.875	26.674999999999997	28.849999999999998
9	22.075	17.025000000000002	32.95	27.950000000000003
10-11	29.6375	22.112499999999997	23.0875	25.162499999999998
12-13	30.012499999999996	19.1875	21.7375	29.062500000000004
14-15	29.375	18.575	24.4875	27.5625
16-17	30.012499999999996	17.4375	25.324999999999996	27.224999999999998
18-19	30.675	18.725	21.625	28.975
20-21	28.8625	19.4875	19.85	31.8
22-23	27.487499999999997	19.787499999999998	23.1	29.625
24-25	29.775000000000002	22.45	19.037499999999998	28.7375
26-27	29.1625	19.787499999999998	20.6125	30.4375
28-29	26.7625	20.0	21.625	31.612499999999997
30-31	25.912499999999998	19.2	22.575	32.3125
32-33	29.5	19.4375	23.175	27.8875
34-35	30.2375	21.075	21.7375	26.950000000000003
36-37	31.1	21.0625	20.8625	26.974999999999998
38-39	28.95	21.6875	21.512500000000003	27.85
40-41	28.325	20.424999999999997	21.6625	29.5875
42-43	28.125	17.849999999999998	24.5	29.525000000000002
44-45	29.812499999999996	18.175	23.974999999999998	28.037499999999998
46-47	30.5125	19.412499999999998	21.3875	28.6875
48-49	28.749999999999996	19.1375	23.5375	28.575
50-51	30.7	20.2125	20.837500000000002	28.249999999999996
52-53	29.575000000000003	20.525	20.175	29.725
54-55	30.75768942235559	17.92948237059265	22.630657664416105	28.68217054263566
56-57	29.119779944986245	17.566891722930734	22.780695173793447	30.532633158289574
58-59	27.31024134050269	20.845316993872704	21.42053269976241	30.4239089658622
60-61	30.823117338003502	19.73980485364023	19.377032774580936	30.060045033775328
62-63	31.88188188188188	20.10760760760761	20.22022022022022	27.790290290290294
64-65	33.145645645645644	18.043043043043046	21.22122122122122	27.59009009009009
66-67	32.040050062578224	18.360450563204004	20.51314142678348	29.086357947434294
68-69	30.14521782674011	18.965948923385078	21.507260891337005	29.381572358537806
70-71	27.77777777777778	19.739152244795584	23.06245297215952	29.42061700526712
72-73	29.75726323732864	18.639164884920138	22.613507734876116	28.99006414287511
74-75	29.614195456378546	16.265627100416722	23.3364699556392	30.783707487565533
76	30.33122441384444	0.0	31.14998139188686	38.5187941942687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.5
28	1.5
29	1.0
30	2.0
31	2.0
32	2.0
33	3.5
34	6.0
35	5.0
36	2.5
37	2.0
38	5.0
39	11.0
40	15.0
41	20.5
42	31.0
43	40.5
44	40.0
45	36.0
46	35.0
47	38.0
48	45.5
49	62.0
50	79.0
51	126.5
52	188.0
53	251.5
54	268.5
55	284.5
56	319.5
57	249.0
58	190.5
59	269.0
60	305.0
61	295.0
62	322.5
63	256.0
64	227.5
65	263.5
66	219.5
67	169.5
68	128.0
69	97.5
70	78.0
71	54.5
72	45.0
73	29.0
74	17.0
75	9.0
76	4.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.15
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
53	1.0
54	0.0
55	0.0
56	0.0
57	0.0
58	1.0
59	1.0
60	0.0
61	1.0
62	0.0
63	0.0
64	0.0
65	1.0
66	0.0
67	0.0
68	2.0
69	5.0
70	2.0
71	4.0
72	13.0
73	78.0
74	343.0
75	861.0
76	2687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.27544910179641	28.599999999999998
2	17.800762112139356	16.35
3	6.695699510070767	9.225
4	4.246053347849755	7.8
5	2.0685900925421885	4.75
6	1.633097441480675	4.5
7	1.3609145345672293	4.375
8	0.6532389765922699	2.4
9	0.7076755579749592	2.9250000000000003
>10	2.558519324986391	19.075
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTC	49	1.225	No Hit
GTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGC	38	0.95	No Hit
CTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAG	30	0.75	No Hit
GCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGC	29	0.7250000000000001	No Hit
CTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTG	28	0.7000000000000001	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAA	27	0.675	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCT	23	0.575	No Hit
CCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	23	0.575	No Hit
GGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	23	0.575	No Hit
GTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGC	23	0.575	No Hit
CTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAG	19	0.475	No Hit
CCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGG	19	0.475	No Hit
CTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGG	18	0.44999999999999996	No Hit
CCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCC	17	0.42500000000000004	No Hit
CCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	16	0.4	No Hit
CTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGT	15	0.375	No Hit
GCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTC	15	0.375	No Hit
GCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTC	14	0.35000000000000003	No Hit
GTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAG	14	0.35000000000000003	No Hit
CTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCT	14	0.35000000000000003	No Hit
GGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAG	13	0.325	No Hit
CTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGG	13	0.325	No Hit
GTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAAT	13	0.325	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTT	13	0.325	No Hit
CCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCT	13	0.325	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	13	0.325	No Hit
GGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAG	13	0.325	No Hit
GTGGTGGTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCT	13	0.325	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	13	0.325	No Hit
GGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCA	12	0.3	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	12	0.3	No Hit
GTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	11	0.27499999999999997	No Hit
CTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCCA	11	0.27499999999999997	No Hit
CCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCC	11	0.27499999999999997	No Hit
GTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATC	11	0.27499999999999997	No Hit
CCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCC	11	0.27499999999999997	No Hit
CCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTG	11	0.27499999999999997	No Hit
GTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCC	11	0.27499999999999997	No Hit
CCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGGAGGGATGGAGTCTG	11	0.27499999999999997	No Hit
GCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGG	10	0.25	No Hit
CTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCT	10	0.25	No Hit
GTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTC	10	0.25	No Hit
GTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGT	10	0.25	No Hit
CCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGG	10	0.25	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTG	10	0.25	No Hit
CTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGG	10	0.25	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	10	0.25	No Hit
CCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGA	9	0.22499999999999998	No Hit
GTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGC	9	0.22499999999999998	No Hit
CGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCC	9	0.22499999999999998	No Hit
GGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAGGC	9	0.22499999999999998	No Hit
GGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCG	9	0.22499999999999998	No Hit
GTCACCATTTCCTGTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCC	9	0.22499999999999998	No Hit
CTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGA	9	0.22499999999999998	No Hit
CACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGA	9	0.22499999999999998	No Hit
CTGCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTA	9	0.22499999999999998	No Hit
GGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCT	9	0.22499999999999998	No Hit
GTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCT	9	0.22499999999999998	No Hit
GCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATC	9	0.22499999999999998	No Hit
CCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGG	9	0.22499999999999998	No Hit
CTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCA	8	0.2	No Hit
GGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCA	8	0.2	No Hit
CTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCC	8	0.2	No Hit
CCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	8	0.2	No Hit
GGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAG	8	0.2	No Hit
GTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGTAC	8	0.2	No Hit
CCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGC	8	0.2	No Hit
GTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGG	8	0.2	No Hit
CCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGG	8	0.2	No Hit
GCCAATTTCACAATCCAGAAGTCCCCGTGCCCTAAAGGGTCTGCCCTGAT	8	0.2	No Hit
CCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTC	8	0.2	No Hit
GCCCAAGTCTGGGTCTGGGGGGAAAGGTGTCATGGAGCCCCCTACGATTC	8	0.2	No Hit
GTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCG	7	0.17500000000000002	No Hit
CCAGCCTTGCACGGAGGGATCTGGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCCAGCAC	7	0.17500000000000002	No Hit
GGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCAC	7	0.17500000000000002	No Hit
CCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGG	7	0.17500000000000002	No Hit
GCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	7	0.17500000000000002	No Hit
TTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTGC	7	0.17500000000000002	No Hit
GTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACG	7	0.17500000000000002	No Hit
GCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTG	7	0.17500000000000002	No Hit
GGGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTC	7	0.17500000000000002	No Hit
TACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGAT	7	0.17500000000000002	No Hit
GCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCT	7	0.17500000000000002	No Hit
CCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCT	7	0.17500000000000002	No Hit
GCCCATGTTCCTCTCCCACATCATCAGGGGCACAGCGTGCACTGTGGGGT	7	0.17500000000000002	No Hit
GTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTG	7	0.17500000000000002	No Hit
GCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCC	7	0.17500000000000002	No Hit
CCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTATGTGCTGTAC	7	0.17500000000000002	No Hit
GTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCAC	7	0.17500000000000002	No Hit
GTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGC	7	0.17500000000000002	No Hit
CTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCT	7	0.17500000000000002	No Hit
GTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGT	7	0.17500000000000002	No Hit
CTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCA	7	0.17500000000000002	No Hit
CCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCAGC	7	0.17500000000000002	No Hit
CCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCAA	7	0.17500000000000002	No Hit
CCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCA	7	0.17500000000000002	No Hit
GGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCC	7	0.17500000000000002	No Hit
CCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGG	6	0.15	No Hit
CCTGGTTAGAAAACTGGGGCCAGCACACAGGGGAAGGGTAAGCTGGTTTC	6	0.15	No Hit
GCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	6	0.15	No Hit
GTCTGGGTCTGGGGGGAAAGGTGTCATGGAGCCCCCTACGATTCCCAGTC	6	0.15	No Hit
CTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGG	6	0.15	No Hit
CCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGA	6	0.15	No Hit
GGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCCAAAG	6	0.15	No Hit
CATGTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGG	6	0.15	No Hit
GTAGTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGAATGGGG	6	0.15	No Hit
CCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGG	6	0.15	No Hit
CCTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCT	6	0.15	No Hit
CATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAG	6	0.15	No Hit
AGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGG	6	0.15	No Hit
GTTGGAATGAATGGCCTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGCCTTGGCCCCC	6	0.15	No Hit
CCCCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTT	6	0.15	No Hit
AGCATGTTTAAGGGGACGGGTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAACGAAAAGCCCACT	6	0.15	No Hit
GGCAGGGCTGGGAGTACCAGCAGGCACTCAAGCGGCTTAAGTGTTCCATGACAGACTGGTATGAAGGTGGCCACA	6	0.15	No Hit
GTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCA	6	0.15	No Hit
GTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCA	6	0.15	No Hit
CTCCAATGGCCTGCACCTGGCTCCGGCTCTGCTCTACCTGCTGAAGATGT	6	0.15	No Hit
GGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAA	6	0.15	No Hit
CCTTGATCTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATG	6	0.15	No Hit
GCAGGATTTTGACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTAT	6	0.15	No Hit
CTCTCTTGGAGCCACCACAGAAAACAGAGGTGCATCCAGCACCACAGAAA	6	0.15	No Hit
CCCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	6	0.15	No Hit
GTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGT	6	0.15	No Hit
GGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATC	6	0.15	No Hit
GTGGAAGGGAGTGAGCTCTCGGACTCCCAGGAGTAAAAGCTTCCAAGTTG	6	0.15	No Hit
CTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAAACAGAGGTGCATCCAGCACCACAGA	6	0.15	No Hit
ACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAG	6	0.15	No Hit
CACGGAGGGATCTGGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCCAGCACACAGGGGA	5	0.125	No Hit
GCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCT	5	0.125	No Hit
GTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCC	5	0.125	No Hit
CTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTCCGTGACGGATGCCTG	5	0.125	No Hit
GCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCACCTGGCTCCGGCT	5	0.125	No Hit
GCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTT	5	0.125	No Hit
CCCTGCTTGCAAGTCCCCTGTCTGTAGCCTCACCCCTGTCGTATCCTGAC	5	0.125	No Hit
GCCAGGAGCTCACCTGCCTGCGTCACTGGGCACAGACGCCAGTGAGGCCAGAGGCCGGGCTGTGCTGGGGCCTG	5	0.125	No Hit
GCCTCTACAAGGATAATCTGACCTGCAGGGTCGAGGAGTTGACGGTGCTGAGTTCCCTGCACTCTCAGTAGGGAC	5	0.125	No Hit
GTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCCTGCTTGCAAGTCC	5	0.125	No Hit
GCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGC	5	0.125	No Hit
TAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCA	5	0.125	No Hit
CCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTC	5	0.125	No Hit
CCTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAAACAGAGGTGCATCCAGCACCAC	5	0.125	No Hit
GGGGAAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTC	5	0.125	No Hit
TGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCT	5	0.125	No Hit
CGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCA	5	0.125	No Hit
CAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACA	5	0.125	No Hit
GGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCAC	5	0.125	No Hit
CCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCC	5	0.125	No Hit
CGGCTTAAGTGTTCCATGACAGACTGGTATGAAGGTGGCCACAATTCAGA	5	0.125	No Hit
GGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCA	5	0.125	No Hit
GTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGA	5	0.125	No Hit
CAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGA	5	0.125	No Hit
CCTCTCTAGCCCCCACCCTGCAGAGCTGGACCCCTGAGCTAGCCATGCTC	5	0.125	No Hit
CCTGTGTGCAGATACTCCCTGCTTCCTCTCTAGCCCCCACCCTGCAGAGC	5	0.125	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCA	5	0.125	No Hit
CAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGC	5	0.125	No Hit
CCCCTCTTCTGCCCTTCATCCTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAAACAG	5	0.125	No Hit
CCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGG	5	0.125	No Hit
GGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCG	5	0.125	No Hit
GTTGGTTTCTGCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAA	5	0.125	No Hit
CTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGAGGAACCTTCCCCAAAC	5	0.125	No Hit
CACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCAC	5	0.125	No Hit
CCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	5	0.125	No Hit
GCTCAGGCAGGGCTGGGGAAGCTTACTGTGTCCAAGAGCCTGCTGGGAGGGAAGTCACCTCCCCTCAAACGAGG	5	0.125	No Hit
CTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAG	5	0.125	No Hit
CCCACATCATCAGGGGCACAGCGTGCACTGTGGGGTCCCAGGCCTCCCGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
ERR5305084 read2 length is 31-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5305084_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	31-76
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.136	38.0	32.0	38.0	32.0	38.0
2	36.06725	38.0	32.0	38.0	32.0	38.0
3	35.8715	38.0	32.0	38.0	32.0	38.0
4	36.00975	38.0	32.0	38.0	32.0	38.0
5	35.8445	38.0	32.0	38.0	32.0	38.0
6	38.45375	40.0	38.0	40.0	38.0	40.0
7	38.436	40.0	38.0	40.0	38.0	40.0
8	38.462	40.0	38.0	40.0	38.0	40.0
9	38.47	40.0	38.0	40.0	38.0	40.0
10-11	38.364625000000004	40.0	38.0	40.0	38.0	40.0
12-13	38.462875	40.0	38.0	40.0	38.0	40.0
14-15	38.383750000000006	40.0	38.0	40.0	38.0	40.0
16-17	38.449375	40.0	38.0	40.0	38.0	40.0
18-19	38.35775	40.0	38.0	40.0	38.0	40.0
20-21	38.438500000000005	40.0	38.0	40.0	38.0	40.0
22-23	38.391375	40.0	38.0	40.0	38.0	40.0
24-25	38.388875	40.0	38.0	40.0	38.0	40.0
26-27	38.420375	40.0	38.0	40.0	38.0	40.0
28-29	38.37575	40.0	38.0	40.0	38.0	40.0
30-31	38.363749999999996	40.0	38.0	40.0	38.0	40.0
32-33	38.40085021255314	40.0	38.0	40.0	38.0	40.0
34-35	38.335583895974	40.0	38.0	40.0	38.0	40.0
36-37	38.36496624156039	40.0	38.0	40.0	38.0	40.0
38-39	38.35183795948987	40.0	38.0	40.0	38.0	40.0
40-41	38.30895223805952	40.0	38.0	40.0	38.0	40.0
42-43	38.28382095523881	40.0	38.0	40.0	38.0	40.0
44-45	38.346461615403854	40.0	38.0	40.0	38.0	40.0
46-47	38.248187046761686	40.0	38.0	40.0	38.0	40.0
48-49	38.251937984496124	40.0	38.0	40.0	38.0	40.0
50-51	38.21142785696424	40.0	38.0	40.0	38.0	40.0
52-53	38.28294573643411	40.0	38.0	40.0	38.0	40.0
54-55	38.20210105052526	40.0	38.0	40.0	38.0	40.0
56-57	38.16470735367684	40.0	38.0	40.0	38.0	40.0
58-59	38.29930711540409	40.0	38.0	40.0	38.0	40.0
60-61	38.20495495495496	40.0	38.0	40.0	38.0	40.0
62-63	38.23210710710711	40.0	38.0	40.0	38.0	40.0
64-65	38.234709166377854	40.0	38.0	40.0	38.0	40.0
66-67	38.19418891673348	40.0	38.0	40.0	38.0	40.0
68-69	38.08903080390684	40.0	38.0	40.0	38.0	40.0
70-71	38.17099133932268	40.0	38.0	40.0	38.0	40.0
72-73	38.214288972839356	40.0	38.0	40.0	38.0	40.0
74-75	38.28699818218114	40.0	38.0	40.0	38.0	40.0
76	37.83359253499222	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	3.0
22	5.0
23	2.0
24	6.0
25	13.0
26	9.0
27	13.0
28	19.0
29	16.0
30	20.0
31	34.0
32	50.0
33	56.0
34	60.0
35	97.0
36	164.0
37	353.0
38	1125.0
39	1954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.65	15.975	6.6000000000000005	42.775
2	28.775000000000002	21.25	25.2	24.775
3	29.549999999999997	20.575	21.175	28.7
4	30.625000000000004	27.400000000000002	17.65	24.325
5	37.7	24.55	15.425	22.325
6	28.299999999999997	30.2	16.1	25.4
7	27.474999999999998	16.7	28.199999999999996	27.625
8	29.099999999999998	18.95	19.825	32.125
9	26.775	19.075	25.1	29.049999999999997
10-11	31.7375	23.175	19.6	25.4875
12-13	31.662499999999998	17.5875	20.0625	30.6875
14-15	28.875	21.325	20.8125	28.9875
16-17	32.2	18.787499999999998	21.8875	27.125
18-19	31.05	20.3375	19.975	28.6375
20-21	30.349999999999998	19.6	19.7	30.349999999999998
22-23	31.4875	18.587500000000002	21.55	28.375
24-25	29.275000000000002	21.7	19.1375	29.8875
26-27	28.6875	20.0375	19.75	31.525
28-29	32.7	18.85	20.3875	28.0625
30-31	29.75	19.7375	20.5125	30.0
32-33	28.33208302075519	20.417604401100277	21.45536384096024	29.794948737184296
34-35	29.719929982495625	20.392598149537385	19.504876219054765	30.38259564891223
36-37	27.26931732933233	20.142535633908476	19.529882470617654	33.05826456614154
38-39	31.807951987997	19.42985746436609	18.97974493623406	29.782445611402853
40-41	30.070017504376096	20.792698174543638	19.42985746436609	29.707426856714182
42-43	30.09004502251126	20.66033016508254	19.15957978989495	30.09004502251126
44-45	30.15753938484621	20.205051262815704	21.005251312828207	28.632158039509875
46-47	31.032758189547387	20.492623155788948	19.454863715928983	29.019754938734682
48-49	29.719929982495625	20.40510127531883	20.355088772193046	29.519879969992495
50-51	29.185944729273476	21.383018631986992	20.220082530949107	29.21095410779042
52-53	29.75743935983996	20.817704426106527	19.56739184796199	29.857464366091524
54-55	26.688344172086044	21.48574287143572	20.522761380690348	31.303151575787897
56-57	27.426213106553277	22.511255627813906	20.12256128064032	29.939969984992498
58-59	28.580362726704188	21.525953721075673	19.349593495934958	30.544090056285178
60-61	26.826826826826828	21.57157157157157	21.246246246246248	30.355355355355357
62-63	28.040540540540544	20.833333333333336	21.283783783783782	29.84234234234234
64-65	30.29165102015271	19.714607585429967	21.17912129177619	28.81462010264113
66-67	31.62639288844372	20.395642919744585	18.141980718667835	29.835983473143855
68-69	30.728775356874532	21.825694966190834	18.19434009516654	29.251189581768095
70-71	29.6449629908418	20.072763768661396	19.33258060469201	30.949692635804794
72-73	31.090428876870835	19.670481700415042	19.179977361338196	30.059112061375927
74-75	32.179170101676284	18.040670513877437	19.771915361363014	30.008244023083265
76	32.93157076205288	0.0	25.0	42.06842923794712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	3.0
26	5.5
27	6.5
28	7.5
29	6.5
30	5.0
31	3.0
32	1.5
33	3.5
34	6.0
35	5.0
36	2.5
37	4.5
38	8.0
39	20.5
40	33.0
41	24.5
42	19.0
43	23.0
44	28.0
45	30.0
46	29.0
47	40.5
48	43.0
49	64.5
50	87.5
51	103.0
52	115.0
53	177.0
54	266.0
55	268.5
56	299.0
57	268.0
58	188.0
59	246.5
60	306.5
61	260.0
62	206.0
63	196.5
64	233.0
65	245.5
66	242.5
67	250.5
68	220.5
69	138.5
70	92.0
71	124.5
72	128.5
73	84.0
74	31.0
75	8.5
76	2.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.025006251562890724
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.012503125781445362
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
31	1.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	1.0
54	0.0
55	0.0
56	0.0
57	0.0
58	1.0
59	1.0
60	0.0
61	0.0
62	0.0
63	1.0
64	1.0
65	0.0
66	1.0
67	0.0
68	0.0
69	6.0
70	3.0
71	2.0
72	13.0
73	124.0
74	412.0
75	861.0
76	2572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.88352120236178	27.425
2	19.32367149758454	18.0
3	8.964036500268383	12.525
4	4.240472356414386	7.9
5	2.4154589371980677	5.625
6	1.556629092860977	4.35
7	0.8051529790660225	2.625
8	0.8588298443370908	3.2
9	0.4294149221685454	1.7999999999999998
>10	2.522812667740204	16.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTC	37	0.9249999999999999	No Hit
CTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGAC	30	0.75	No Hit
CCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCA	24	0.6	No Hit
CCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCT	23	0.575	No Hit
CGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTT	19	0.475	No Hit
CCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACA	17	0.42500000000000004	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	17	0.42500000000000004	No Hit
CAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCA	16	0.4	No Hit
GTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCT	16	0.4	No Hit
GGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCC	15	0.375	No Hit
CAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTAGA	15	0.375	No Hit
CTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAAC	14	0.35000000000000003	No Hit
GCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCA	14	0.35000000000000003	No Hit
CGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCT	14	0.35000000000000003	No Hit
CAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAG	14	0.35000000000000003	No Hit
CCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTG	14	0.35000000000000003	No Hit
CTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCC	14	0.35000000000000003	No Hit
GGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACC	14	0.35000000000000003	No Hit
GTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTA	14	0.35000000000000003	No Hit
GGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCT	14	0.35000000000000003	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	13	0.325	No Hit
CTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGA	13	0.325	No Hit
GCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTG	13	0.325	No Hit
CAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAG	13	0.325	No Hit
CTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGC	13	0.325	No Hit
AGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGC	13	0.325	No Hit
GCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCA	13	0.325	No Hit
CCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCC	13	0.325	No Hit
CCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGC	12	0.3	No Hit
ACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGG	12	0.3	No Hit
CTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCT	12	0.3	No Hit
GTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGA	12	0.3	No Hit
GTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTC	11	0.27499999999999997	No Hit
AGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAG	11	0.27499999999999997	No Hit
ATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTA	11	0.27499999999999997	No Hit
CGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCAC	11	0.27499999999999997	No Hit
CTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGC	11	0.27499999999999997	No Hit
CCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCT	10	0.25	No Hit
GCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCT	10	0.25	No Hit
GGAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATG	10	0.25	No Hit
CAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGA	10	0.25	No Hit
CGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	10	0.25	No Hit
CTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAG	10	0.25	No Hit
GCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	10	0.25	No Hit
GGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAG	10	0.25	No Hit
GATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACC	10	0.25	No Hit
AGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTA	10	0.25	No Hit
GAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAAC	9	0.22499999999999998	No Hit
CCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGG	9	0.22499999999999998	No Hit
GGATTACTCAAGACAGGACTTCAAGTTGATTCAGGCGTGTTAGGGAGCTGTGATTGGATTTTGAGCAGGGCAGGG	9	0.22499999999999998	No Hit
CTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAG	9	0.22499999999999998	No Hit
GCAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGA	9	0.22499999999999998	No Hit
GACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGG	9	0.22499999999999998	No Hit
TGCAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAG	9	0.22499999999999998	No Hit
CAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGA	9	0.22499999999999998	No Hit
CTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGG	8	0.2	No Hit
GGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTG	8	0.2	No Hit
GGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGA	8	0.2	No Hit
CTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGA	8	0.2	No Hit
GTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTC	8	0.2	No Hit
CCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTC	8	0.2	No Hit
GACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGC	8	0.2	No Hit
CCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACG	8	0.2	No Hit
GAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCT	8	0.2	No Hit
ACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	8	0.2	No Hit
TGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCA	8	0.2	No Hit
CTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGG	8	0.2	No Hit
CAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCA	8	0.2	No Hit
GGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGC	8	0.2	No Hit
CTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTG	8	0.2	No Hit
GGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCA	8	0.2	No Hit
AGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGA	7	0.17500000000000002	No Hit
GTCCAGCAGATGGCGGATGCCCTGCAGTACCTGCAGAAGGTCTCTGGAGA	7	0.17500000000000002	No Hit
CTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCC	7	0.17500000000000002	No Hit
CGGATTACTCAAGACAGGACTTCAAGTTGATTCAGGCGTGTTAGGGAGCT	7	0.17500000000000002	No Hit
CTCCAAGAGAGTGCACGGTCCCTGCTGATTGAAAGAAGGATGAAGGGCAG	7	0.17500000000000002	No Hit
CACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTC	7	0.17500000000000002	No Hit
GGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCA	7	0.17500000000000002	No Hit
TGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACC	7	0.17500000000000002	No Hit
CAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGC	7	0.17500000000000002	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTAGA	7	0.17500000000000002	No Hit
GAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCA	7	0.17500000000000002	No Hit
GGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATC	7	0.17500000000000002	No Hit
CCTAAATGTTGGGCTTCACATTTGTTCCTTGTAAATTTCATCTTGGTGATTGCAGTCTACCCTCTGGCCTTTAAA	7	0.17500000000000002	No Hit
AGCTGAAGGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGAC	7	0.17500000000000002	No Hit
GGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGA	7	0.17500000000000002	No Hit
ACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGT	6	0.15	No Hit
GGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGT	6	0.15	No Hit
CTGGAGGCTGTCCTTCAGATAGCATGTACAGGTGGCAGCATAGGGCCTGTCCCTACTGAGAGTGCAGGGAACTC	6	0.15	No Hit
CAGTCACCCTCTGTTTTCTGTGGTGGCTCTGTTTTCTGTGGTGCTGGATGCACCTCTGTTTTCTGTGGTGGCTCC	6	0.15	No Hit
AGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGAC	6	0.15	No Hit
CAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCA	6	0.15	No Hit
CTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCC	6	0.15	No Hit
GGGTGATGGAACCAGCACTGTGCGGAGACCAGCTTCAAGGAGCGGAAGGC	6	0.15	No Hit
GGACAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCC	6	0.15	No Hit
GGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGGACACCCAGAAGCT	6	0.15	No Hit
GCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGT	6	0.15	No Hit
CCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGA	6	0.15	No Hit
CCTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCG	6	0.15	No Hit
GGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTT	6	0.15	No Hit
CATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCC	6	0.15	No Hit
CCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCC	6	0.15	No Hit
GAAGGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATG	6	0.15	No Hit
TGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCG	6	0.15	No Hit
TGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTC	6	0.15	No Hit
GGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGACACC	6	0.15	No Hit
CACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGG	6	0.15	No Hit
GGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAG	6	0.15	No Hit
TGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCG	6	0.15	No Hit
CAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGG	6	0.15	No Hit
GCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCA	6	0.15	No Hit
AGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGC	6	0.15	No Hit
GTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGAC	6	0.15	No Hit
CCATTGTTCAACTGTGCCCTTTGGGGCTGTGGTTCATTTGGCTCTGATTT	6	0.15	No Hit
CTAGCATCCAGAAGTGGCACAAAACCCCTCTGCTGGCTCGTGTGTGCAAC	6	0.15	No Hit
CTCTGCTAGAGTCCATCCGCCAAGCTGGGGGCATCGGCAAGGCCAAGCTGCGCAGCATGAAGGAGCGAAAGCTGG	5	0.125	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	5	0.125	No Hit
CCCAGAAGCTGTTATTGTAGTCAGGATACGACAGGGGTGAGGCTACAGACAGGGGACTTGCAAGCAGGGAGGGC	5	0.125	No Hit
CCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATCTTCACGGGCGCCC	5	0.125	No Hit
AGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGC	5	0.125	No Hit
AGGTGCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTACATTGCCG	5	0.125	No Hit
CTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGGACT	5	0.125	No Hit
TGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	5	0.125	No Hit
GCTCCATCTTCACCGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGC	5	0.125	No Hit
CGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTG	5	0.125	No Hit
CGGGTGGCTCGGCTCGGGAGGCCTGGGACCCCACAGTGCACGCTGTGCCC	5	0.125	No Hit
CCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGC	5	0.125	No Hit
CAACACCACCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGG	5	0.125	No Hit
GGATGACAGTCACCCTCTGTTTTCTGTGGTGGCTCTGTTTTCTGTGGTGC	5	0.125	No Hit
GGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGCAGCTGG	5	0.125	No Hit
GAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTC	5	0.125	No Hit
GTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACA	5	0.125	No Hit
GCCTGACCTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGG	5	0.125	No Hit
TGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTA	5	0.125	No Hit
GGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACC	5	0.125	No Hit
CAAAACCCCTCTGCTGGCTCGTGTGTGCAACTGAGACTGTCAGAGCATGG	5	0.125	No Hit
CACCACCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTG	5	0.125	No Hit
CTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACC	5	0.125	No Hit
CCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAG	5	0.125	No Hit
GTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGT	5	0.125	No Hit
AAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTG	5	0.125	No Hit
CCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGA	5	0.125	No Hit
CCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCC	5	0.125	No Hit
GATCCCTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTGTGCCCTTT	5	0.125	No Hit
GGAGGCCGTCCAGCAGATGGCGGATGCCCTGCAGTACCTGCAGAAGGTCT	5	0.125	No Hit
GGCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGC	5	0.125	No Hit
GCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCC	5	0.125	No Hit
CCTGTATGGCCAGAGGGCAGGGCCGAGGGGTGTGGGCGGGAGGCCCGGCCTGGCTTAGTGGGGACCCAGGGCATC	5	0.125	No Hit
CTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCT	5	0.125	No Hit
CCCACGGATTACTCAAGACAGGACTTCAAGTTGATTCAGGCGTGTTAGGG	5	0.125	No Hit
CATCAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGG	5	0.125	No Hit
CCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAG	5	0.125	No Hit
CACATTTGTTCCTTGTAAATTTCATCTTGGTGATTGCAGTCTACCCTCTGGCCTTTAAAAATTGTCTGAGCCTTG	5	0.125	No Hit
GCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCG	5	0.125	No Hit
GTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCA	5	0.125	No Hit
CCCAGACCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGG	5	0.125	No Hit
GTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGG	5	0.125	No Hit
GCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCA	5	0.125	No Hit
GCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGC	5	0.125	No Hit
GTCTGAGCCTTGATTCGATCATGAAACCAGCTTACCCTTCCCCTGTGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2025-05-07T14:54:40 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open CM000676.2 as a RefSeq or as a WGS
2025-05-07T14:54:40 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed ERR5305084.sra
Read 7109298 spots for ERR5305084.sra
Written 7109298 spots for ERR5305084.sra

=============================================================
An error occurred during processing.
A report was generated into the file '/home/ziemannm/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
Read 7109298 spots for ERR5305084.sra
Written 7109298 spots for ERR5305084.sra
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='8'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='9'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='9'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - failed ERR5305084.sra
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='8'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='9'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - failed ERR5305084.sra
2025-05-07T14:56:48 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - failed ERR5305084.sra
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='8'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='9'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - system bad file descriptor error fd='7'
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - failed ERR5305084.sra
2025-05-07T14:56:50 fastq-dump.3.0.1 err: storage exhausted while writing file within file system module - failed ERR5305084.sra

=============================================================
An error occurred during processing.
A report was generated into the file '/home/ziemannm/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3

=============================================================
An error occurred during processing.
A report was generated into the file '/home/ziemannm/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3

=============================================================
An error occurred during processing.
A report was generated into the file '/home/ziemannm/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3

=============================================================
An error occurred during processing.
A report was generated into the file '/home/ziemannm/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3

=============================================================
An error occurred during processing.
A report was generated into the file '/home/ziemannm/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
SRR ids: ['ERR5305084.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_0rhc7s49
ERR5305084.sra spots: 56874389
blocks: [[1, 7109298], [7109299, 14218596], [14218597, 21327894], [21327895, 28437192], [28437193, 35546490], [35546491, 42655788], [42655789, 49765086], [49765087, 56874389]]
fastq-dump error! exit code: 3
ERR5305084 file size 1337403
ERR5305084 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR5305084 ERR5305084_1.fastq ERR5305084_2.fastq
Input file:	ERR5305084_1.fastq
Paired file:	ERR5305084_2.fastq
trimmed:	ERR5305084-trimmed-pair1.fastq, ERR5305084-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Wed May  7 14:58:36 2025 >> started

Wed May  7 14:58:46 2025 >> done (10.317s)
7109298 read pairs processed; of these:
      0 ( 0.00%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
7109298 (100.00%) read pairs available; of these:
    224 ( 0.00%) trimmed read pairs available after processing
7109074 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 25	      2	  0.00%
 26	      2	  0.00%
 27	      7	  0.00%
 28	      5	  0.00%
 29	     10	  0.00%
 30	     18	  0.00%
 31	     20	  0.00%
 32	     18	  0.00%
 33	     34	  0.00%
 34	     27	  0.00%
 35	     23	  0.00%
 36	     24	  0.00%
 37	     43	  0.00%
 38	     54	  0.00%
 39	     70	  0.00%
 40	     56	  0.00%
 41	     65	  0.00%
 42	     84	  0.00%
 43	     94	  0.00%
 44	    103	  0.00%
 45	    110	  0.00%
 46	    144	  0.00%
 47	    126	  0.00%
 48	    155	  0.00%
 49	    229	  0.00%
 50	    320	  0.00%
 51	    464	  0.01%
 52	    473	  0.01%
 53	    489	  0.01%
 54	    559	  0.01%
 55	    595	  0.01%
 56	    603	  0.01%
 57	    673	  0.01%
 58	    753	  0.01%
 59	    879	  0.01%
 60	    944	  0.01%
 61	   1174	  0.02%
 62	   1292	  0.02%
 63	   1479	  0.02%
 64	   1645	  0.02%
 65	   2002	  0.03%
 66	   2143	  0.03%
 67	   2420	  0.03%
 68	   2871	  0.04%
 69	   3686	  0.05%
 70	   5054	  0.07%
 71	   7491	  0.11%
 72	  14300	  0.20%
 73	  77180	  1.09%
 74	 645231	  9.08%
 75	3377669	 47.51%
 76	2955386	 41.57%
7109298 reads passed initial QC


criterion=sequence-density
sequence-density=0.24
sequence-density-rank=1
fanout-score=10.53
fanout-score-rank=14
prefix-density=0.81
prefix-fanout=3.1
sequence=CTTCTTCTTCTTGGGGGTCTCCTTGGGGGGGACCTCCCCCTTGGCCTCAGCACCCTGGCTAGGGGGTGCTGGCTCGATGGCATCGCCAGTGGCCCCGGCTGCCTCATCTGTTCCGTTCACAGGGGGCGACTCCCCTTCACCCTTGGGGGATAAGTCTCCATTGCTTTTCACGTGGCCATTCTCCTGGCCGTTGGCCTTCGCGGGGGAAGCGCCTGCTGCCTCCTCGGCGGTCACGTCGCCCCGGGGAGCCTTGGAGCTCTGGCTGCCCATGATGGGGGTCTGCTGGGGGGCGCTTGGAGCCGCCCCGGGCTCGCGCCGCAGGGGATAGTACGGCGGGGTCGGCCCGGCCGGCGGAGGGGTGGGGCTGGCGCCCGAGGGGGAGGGGTGCCGGGGGAAGCCGAGCTGCACC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=46
fanout-score=66.47
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=5.4
sequence=AGGGGCTCTATGGCTTGATTTATTAACCTAACTCAAAAGAAGTCACTGCCACCAACAGCACTGTGCAGTTTTATTAACCATTCAAGTCCAGTAGCATCTGGTAAGATTGGGACAGAATTGGGATTGAAAAGTGAACAGATATTCAGCATCTAACAGTTCAAAAGAAGCCACTACATACTCTTTTCACAAATATGTTTTCACAGAGCCAATACAGTACTAGCCATTAACCCAGTACACCAAGTGTACTGAAGTAGAAAAGATGCAACAAGAAAAATAGCTACATTAGAAAGACCAACACTTTAGAAAAAGAGTAAAACACTTTCAGTTTCTCCCCTTTAGCCCCTAAAACAACATCTTACAGTCTGGATCTGGATCTACCTATACAGTCCTACATTAGCTTCTAAAATATTTGTCAGGAGGGAAAAAATAAAATGACACTGGCCAGTACAGTCTTTGGATATTTAGGAAGGGGATGGGGAGAAAGTCAGTTCTCAGAACAAATTAGTCAGCT


criterion=sequence-density
sequence-density=0.44
sequence-density-rank=1
fanout-score=5.09
fanout-score-rank=27
prefix-density=0.64
prefix-fanout=3.5
sequence=AGGCCAAGGGGGCAGAGGCTAGTGCAGCCTCAGAAGAAGAGGCAGGGCCCCAGGCTACAGAGCCATCCACTCCCTCGGGGCCGGAGAGTGGCCCTACACCAGCCAGCGCTGAGCAGAATGAGTAGCTAGGTAGGGGCAGGTGGGTGATCTCTAAGCTGCAAAAACTGTGCTGTCCTTGTGAGGTCACTGCCTGGACCTGGTGCCCTGGCTGCCTTCCTGTGCCCAGAAAGGAAGGGGCTATTGCCTCCTCCCAGCCACGTTCCCTTTCCTCCTCTCCCTCCTGTGGATTCTCCCATCAGCCATCTGGTTCTCCTCTTAAGGCCAGTTGAAGATGGTCCCTTACAGCTTCCCAAGTTAGGTTAGTGATGTGAAATGCTCCTGTCCCTGGCCCTACCTCCTTCCCTGTCCCCACCCCTGCATAAGGCAGTTGTTGGTTTTCTTCCCCAATTCTTTTCCAAGTAGGTTTTGTTTACCCTACTCCCCAAATCCCTGAGCCAGAAGTGGGGTGCTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=203.86
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=10.0
sequence=CCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTCGCACTGATTTTTTACCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAAATAAACCCTCGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATCCATAATCCTTCTAATAGCTATCCTCTTCAACAATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGC
ERR5305084 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 07 15:00:31
                             Started mapping on |	May 07 15:00:32
                                    Finished on |	May 07 15:01:12
       Mapping speed, Million of reads per hour |	639.84

                          Number of input reads |	7109298
                      Average input read length |	150
                                    UNIQUE READS:
                   Uniquely mapped reads number |	6804179
                        Uniquely mapped reads % |	95.71%
                          Average mapped length |	150.57
                       Number of splices: Total |	3578368
            Number of splices: Annotated (sjdb) |	3536974
                       Number of splices: GT/AG |	3532561
                       Number of splices: GC/AG |	39364
                       Number of splices: AT/AC |	3443
               Number of splices: Non-canonical |	3000
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.58
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.38
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	305032
             % of reads mapped to multiple loci |	4.29%
        Number of reads mapped to too many loci |	8
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.00%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	87	87	87
N_multimapping	305032	305032	305032
N_noFeature	470528	6511584	515061
N_ambiguous	388238	6017	141671
UnstrandedReadsAssigned:5945413 PositiveStrandReadsAssigned:286578 NegativeStrandReadsAssigned:6147447
Dataset is classified negative stranded
MeadianReadLen=76 20thPercentileLength=75 echo kmer=71
ERR5305084 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in paired-end mode
[quant] will process pair 1: ERR5305084-trimmed-pair1.fastq
                             ERR5305084-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 7,109,298 reads, 6,424,765 reads pseudoaligned
[quant] estimated average fragment length: 157.72
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,153 rounds

 180870 ERR5305084.ke.tsv
  58302 ERR5305084.se.tsv
 239172 total
==> ERR5305084.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	4	0	0
ENST00000448914.1	13	8	0	0
ENST00000415118.1	8	3	0	0
ENST00000631435.1	12	7	0	0
ENST00000632684.1	12	7	0	0
ENST00000454908.1	17	12	0	0
ENST00000390567.1	20	9.4	0	0
ENST00000603326.1	19	14	0	0
ENST00000414852.1	16	11	0	0

==> ERR5305084.se.tsv <==
ENSG00000223972	0
ENSG00000227232	131
ENSG00000278267	21
ENSG00000243485	2
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	10
ERR5305084 completed mapping pipeline successfully
