Starting /dee2/code/volunteer_pipeline.sh ERR5303525
    current disk space = 1369990400
    free memory = 120886544 
ERR5303525 SRAfilesize
790236c345c9db73c369deb5035e668e  ERR5303525.sra
ERR5303525.sra file validated
ERR5303525 is paired end
ERR5303525 is conventional basespace
ERR5303525 read1 length is 38-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5303525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38-76
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.82225	38.0	38.0	38.0	32.0	38.0
2	36.792	38.0	38.0	38.0	32.0	38.0
3	36.884	38.0	38.0	38.0	32.0	38.0
4	36.6985	38.0	38.0	38.0	32.0	38.0
5	36.6465	38.0	38.0	38.0	32.0	38.0
6	37.47725	38.0	38.0	40.0	32.0	40.0
7	37.9555	38.0	38.0	40.0	38.0	40.0
8	38.26875	38.0	38.0	40.0	38.0	40.0
9	38.54775	40.0	38.0	40.0	38.0	40.0
10-11	38.516375	40.0	38.0	40.0	38.0	40.0
12-13	38.56175	40.0	38.0	40.0	38.0	40.0
14-15	38.575374999999994	40.0	38.0	40.0	38.0	40.0
16-17	38.588750000000005	40.0	38.0	40.0	38.0	40.0
18-19	38.546375	40.0	38.0	40.0	38.0	40.0
20-21	38.455375000000004	40.0	38.0	40.0	38.0	40.0
22-23	38.470875	40.0	38.0	40.0	38.0	40.0
24-25	38.464124999999996	40.0	38.0	40.0	38.0	40.0
26-27	38.4575	40.0	38.0	40.0	38.0	40.0
28-29	38.416875000000005	40.0	38.0	40.0	38.0	40.0
30-31	38.378375	40.0	38.0	40.0	38.0	40.0
32-33	38.339625	40.0	38.0	40.0	38.0	40.0
34-35	38.285	40.0	38.0	40.0	38.0	40.0
36-37	38.280625	40.0	38.0	40.0	38.0	40.0
38-39	38.20602063015754	40.0	38.0	40.0	38.0	40.0
40-41	38.227181795448864	40.0	38.0	40.0	38.0	40.0
42-43	38.25106276569142	40.0	38.0	40.0	38.0	40.0
44-45	38.22880720180045	40.0	38.0	40.0	38.0	40.0
46-47	38.2646911727932	40.0	38.0	40.0	38.0	40.0
48-49	38.23068267066767	40.0	38.0	40.0	38.0	40.0
50-51	38.24693673418355	40.0	38.0	40.0	38.0	40.0
52-53	38.20717679419855	40.0	38.0	40.0	38.0	40.0
54-55	38.23759207060394	40.0	38.0	40.0	38.0	40.0
56-57	38.2383691845923	40.0	38.0	40.0	38.0	40.0
58-59	38.135067533766886	40.0	38.0	40.0	38.0	40.0
60-61	38.11783002459448	40.0	38.0	40.0	38.0	40.0
62-63	38.14364364364364	40.0	38.0	40.0	38.0	40.0
64-65	38.11536900101493	40.0	38.0	40.0	38.0	40.0
66-67	38.085517853899326	40.0	38.0	40.0	38.0	40.0
68-69	37.976101635499006	40.0	38.0	40.0	38.0	40.0
70-71	38.081270825609224	40.0	38.0	40.0	38.0	40.0
72-73	38.122974229014375	40.0	38.0	40.0	38.0	40.0
74-75	38.16665419548867	40.0	38.0	40.0	38.0	40.0
76	38.074298711144806	38.0	38.0	40.0	38.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	1.0
23	3.0
24	4.0
25	6.0
26	8.0
27	16.0
28	13.0
29	26.0
30	20.0
31	38.0
32	57.0
33	73.0
34	81.0
35	102.0
36	186.0
37	348.0
38	927.0
39	2090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.90872718179545	7.701925481370342	5.176294073518379	52.213053263315835
2	21.349999999999998	8.5	35.575	34.575
3	22.975	9.825000000000001	21.9	45.300000000000004
4	25.650000000000002	15.75	21.2	37.4
5	27.802802802802802	19.91991991991992	24.074074074074073	28.203203203203202
6	28.93223305826457	21.630407601900476	22.655663915978995	26.78169542385596
7	24.525	17.150000000000002	34.35	23.974999999999998
8	25.224999999999998	18.65	26.900000000000002	29.225
9	23.0	14.75	32.7	29.549999999999997
10-11	28.1625	21.987499999999997	22.275	27.575
12-13	28.012500000000003	19.425	23.1875	29.375
14-15	27.8125	18.1625	24.9375	29.0875
16-17	26.775	18.45	25.775	28.999999999999996
18-19	29.725	18.2	21.912499999999998	30.162499999999998
20-21	26.137500000000003	20.375	21.425	32.0625
22-23	27.474999999999998	19.475	21.8625	31.1875
24-25	28.849999999999998	20.9	20.4875	29.762499999999996
26-27	27.175	19.662499999999998	20.7	32.4625
28-29	26.3	20.3125	22.162499999999998	31.225
30-31	24.05	19.3875	23.225	33.3375
32-33	27.9125	18.575	24.075	29.4375
34-35	28.275	20.1	22.537499999999998	29.0875
36-37	30.0	19.787499999999998	22.15	28.0625
38-39	29.278659832479057	20.040005000625076	23.19039879984998	27.490936367045883
40-41	28.073027385269476	19.357258972114543	21.30799049643616	31.261723146179815
42-43	28.744686171542888	18.642160540135034	23.10577644411103	29.507376844211052
44-45	28.707176794198553	18.967241810452613	23.893473368342086	28.432108027006752
46-47	29.557389347336834	18.779694923730933	21.530382595648913	30.132533133283324
48-49	29.28598224334125	18.69451044141553	23.571339252219584	28.448168063023633
50-51	29.923721395523323	19.344754282856073	21.533074903088657	29.19844941853195
52-53	28.632158039509875	20.84271067766942	20.455113778444613	30.070017504376096
54-55	28.973365011879455	18.682005752157057	22.05827185194448	30.28635738401901
56-57	26.788394197098548	18.25912956478239	23.36168084042021	31.59079539769885
58-59	27.288644322161083	20.16008004002001	21.57328664332166	30.977988994497245
60-61	29.443402126328955	20.012507817385867	19.299562226391494	31.24452782989368
62-63	30.26776776776777	19.21921921921922	20.933433433433432	29.57957957957958
64-65	31.667292997619946	17.700112739571587	21.55831141174997	29.0742828510585
66-67	30.72289156626506	18.38604417670683	20.95883534136546	29.93222891566265
68-69	30.49056603773585	19.270440251572328	20.427672955974842	29.81132075471698
70-71	29.184549356223176	18.15198182277203	21.64857359252714	31.014895228477656
72-73	28.984038510260955	18.647073726881175	21.966050164682038	30.40283759817583
74-75	31.43520415130411	16.37307114570531	21.958213846784105	30.233510856206475
76	29.909021986353295	0.0	27.141774071266113	42.94920394238059
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	1.0
26	1.0
27	1.5
28	2.0
29	1.5
30	2.0
31	2.5
32	1.5
33	3.5
34	3.0
35	1.5
36	4.0
37	5.5
38	9.5
39	22.0
40	36.0
41	44.0
42	49.5
43	56.5
44	55.0
45	43.5
46	40.0
47	36.5
48	34.0
49	57.5
50	90.0
51	121.0
52	157.5
53	231.0
54	265.5
55	272.5
56	267.0
57	207.5
58	197.0
59	266.0
60	300.0
61	275.0
62	280.5
63	232.5
64	231.5
65	272.5
66	233.0
67	204.5
68	144.5
69	96.0
70	80.0
71	56.0
72	42.0
73	42.5
74	36.0
75	16.0
76	12.0
77	14.0
78	10.0
79	2.5
80	5.5
81	3.5
82	7.0
83	12.5
84	5.5
85	1.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.1
6	0.025
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.012503125781445362
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.012503125781445362
50-51	0.012503125781445362
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
38	1.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	1.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	1.0
61	1.0
62	0.0
63	3.0
64	3.0
65	3.0
66	6.0
67	4.0
68	4.0
69	10.0
70	4.0
71	5.0
72	14.0
73	108.0
74	341.0
75	853.0
76	2638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.043269230769226	21.65
2	22.05528846153846	18.35
3	9.314903846153847	11.625
4	5.168269230769231	8.6
5	4.266826923076923	8.875
6	1.9831730769230769	4.95
7	1.0817307692307692	3.15
8	1.0216346153846154	3.4000000000000004
9	0.6009615384615385	2.25
>10	2.4639423076923075	17.150000000000002
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTC	35	0.8750000000000001	No Hit
CCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGG	33	0.8250000000000001	No Hit
CCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	32	0.8	No Hit
GCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGC	31	0.775	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCT	27	0.675	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAA	26	0.65	No Hit
CTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGT	26	0.65	No Hit
CTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTG	25	0.625	No Hit
CTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGG	25	0.625	No Hit
CCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGC	20	0.5	No Hit
GTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGT	19	0.475	No Hit
CCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTC	19	0.475	No Hit
CCTGTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCT	19	0.475	No Hit
GGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAAC	19	0.475	No Hit
CTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAG	18	0.44999999999999996	No Hit
CTGCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTA	16	0.4	No Hit
GTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	15	0.375	No Hit
GGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCA	15	0.375	No Hit
CTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAG	15	0.375	No Hit
CCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATCCACTCTCTCTCC	14	0.35000000000000003	No Hit
CGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCC	13	0.325	No Hit
GTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGC	13	0.325	No Hit
CCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCT	13	0.325	No Hit
CCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGGAGGGATGGAGTCTG	13	0.325	No Hit
CCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCC	12	0.3	No Hit
GTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGG	12	0.3	No Hit
GTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATC	12	0.3	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCA	12	0.3	No Hit
CCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	12	0.3	No Hit
GCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGC	11	0.27499999999999997	No Hit
GGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAG	11	0.27499999999999997	No Hit
GTGGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAG	11	0.27499999999999997	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	11	0.27499999999999997	No Hit
CCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGG	11	0.27499999999999997	No Hit
GGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAG	10	0.25	No Hit
CTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCA	10	0.25	No Hit
CCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGG	10	0.25	No Hit
CAGGAAAGTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGG	10	0.25	No Hit
CTCCCTTCTTGTCCCTTCCGTGACGGATGCCTGAGGAACCTTCCCCAAAC	10	0.25	No Hit
CTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCA	10	0.25	No Hit
GGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAGGCT	10	0.25	No Hit
GTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAACGAAAAG	9	0.22499999999999998	No Hit
GTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGC	9	0.22499999999999998	No Hit
CCTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAAACAGAGGTGCATCCAGCACCAC	9	0.22499999999999998	No Hit
GCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTG	9	0.22499999999999998	No Hit
GTCACCATTTCCTGTCGTCGTTTCCTCTGAATGTCTCACCCTGCCCTCCC	9	0.22499999999999998	No Hit
CTCCAATGGCCTGCACCTGGCTCCGGCTCTGCTCTACCTGCTGAAGATGT	9	0.22499999999999998	No Hit
CCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAG	9	0.22499999999999998	No Hit
CCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGC	9	0.22499999999999998	No Hit
GTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGC	9	0.22499999999999998	No Hit
CTCCCTGCTTGCAAGTCCCCTGTCTGTAGCCTCACCCCTGTCGTATCCTG	9	0.22499999999999998	No Hit
ATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCA	8	0.2	No Hit
GTCACAACTGTTATTGAAGAACTAATCATCTATTGCTTATTTAGGTCTTTCTCTCCTGCCAGAATGTGCGCTCC	8	0.2	No Hit
CTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCC	8	0.2	No Hit
CCCGTGCTTTCTGCTCTGCAGACCCTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTC	8	0.2	No Hit
CCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGG	8	0.2	No Hit
ACCCCATCTTGTAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTG	8	0.2	No Hit
GGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCA	8	0.2	No Hit
CGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCTCC	8	0.2	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	8	0.2	No Hit
GTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGTAC	8	0.2	No Hit
CCCCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTCCCTGGCATC	8	0.2	No Hit
CCAGCCTTCCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTG	8	0.2	No Hit
GTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGT	8	0.2	No Hit
GGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATC	8	0.2	No Hit
CTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCA	8	0.2	No Hit
CTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCT	8	0.2	No Hit
GCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTC	8	0.2	No Hit
CTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCTTCT	7	0.17500000000000002	No Hit
GCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTT	7	0.17500000000000002	No Hit
GCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCT	7	0.17500000000000002	No Hit
CCAGCCTTGCACGGAGGGATCTGGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCCAGCAC	7	0.17500000000000002	No Hit
GTCCCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTG	7	0.17500000000000002	No Hit
CCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACACACACAG	7	0.17500000000000002	No Hit
CTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGGC	7	0.17500000000000002	No Hit
GGCCTCCATTCTAAGTGCTGGACATGGGGTGGCCATAATCTGGAGCTGAT	7	0.17500000000000002	No Hit
CTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCT	7	0.17500000000000002	No Hit
CCCTGGCATCCACTCTCTCTCCCTTCTTGTCCCTTCCGTGACGGATGCCT	7	0.17500000000000002	No Hit
CCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGG	7	0.17500000000000002	No Hit
CCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTG	7	0.17500000000000002	No Hit
GTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCC	7	0.17500000000000002	No Hit
GTGGAAGGGAGTGAGCTCTCGGACTCCCAGGAGTAAAAGCTTCCAAGTTG	7	0.17500000000000002	No Hit
GTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCT	7	0.17500000000000002	No Hit
ACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAG	7	0.17500000000000002	No Hit
CCTTCATCCTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAAACAGAGGTGCATCCAGC	7	0.17500000000000002	No Hit
CTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGT	7	0.17500000000000002	No Hit
GGGGCTAAAAGCATGTTAATCGGCATAAAAAGGCAATGAGATCTTAGGGC	6	0.15	No Hit
GCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGG	6	0.15	No Hit
CTTCTACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGC	6	0.15	No Hit
GGCAGGTAGGATGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCAC	6	0.15	No Hit
CCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGA	6	0.15	No Hit
GGGGAAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTC	6	0.15	No Hit
GCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGAAAGGTGTCATGGAGCCCCCTACGATTCCC	6	0.15	No Hit
GGGGGCGGTGGGGGTGGTGTTAGTACCCCATCTTGTAGGTCTTGAGAGGC	6	0.15	No Hit
CCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTC	6	0.15	No Hit
CCGCTGTCCTGAGCCCATGTTCCTCTCCCACATCATCAGGGGCACAGCGTGCACTGTGGGGTCCCAGGCCTCCCG	6	0.15	No Hit
CTACTGATGGGGCAAGCACTTCACAACCCCTCATGATCACGTGCAGCAGA	6	0.15	No Hit
CTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGG	6	0.15	No Hit
GCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTT	6	0.15	No Hit
CCCATCTTGGCAAGGAAACACAATTTCCGAGGGAATGGTTTTGGCCTCCA	6	0.15	No Hit
CAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCAATGT	6	0.15	No Hit
CTCCCAGTCGCCCCTGTAGCTCCCCTACCTCCAAGAGCCCAGCCCTTGCCCACAGGGCCACACTCCACGTGCAG	6	0.15	No Hit
GCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCA	6	0.15	No Hit
TGGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATATAGGAAAAATCAG	6	0.15	No Hit
CTTCCGAGCGTCAGGCCGCCCCTACCCGTGCTTTCTGCTCTGCAGACCCT	6	0.15	No Hit
GTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCA	6	0.15	No Hit
CAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCT	6	0.15	No Hit
GGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGAAAG	6	0.15	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTT	6	0.15	No Hit
GGACGGGTTCAAAGCTGGTCACATCCCCACCGAAAAAGCCCATGGACAAC	6	0.15	No Hit
GGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCA	6	0.15	No Hit
CCCACCGAAAAAGCCCATGGACAACGAAAAGCCCACTAGCTTGTCCAGTGCCACAGGAGGGGCAAGTGGAGGAGG	6	0.15	No Hit
CCCCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCA	6	0.15	No Hit
TGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGA	6	0.15	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	6	0.15	No Hit
GTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGAAAGGTGTCATGGAGCCCCCTACG	6	0.15	No Hit
CTCTTCCTAGACCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCT	6	0.15	No Hit
CTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAG	6	0.15	No Hit
CTCAGCCTACCTGACCTTGTCTTTACCTCTGTGGGCAGCTCCCTTGTGATCTGCTTAGTTCCCACCCCCCTTTA	6	0.15	No Hit
CACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGC	5	0.125	No Hit
CATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCT	5	0.125	No Hit
GTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTC	5	0.125	No Hit
CTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGGGGGCA	5	0.125	No Hit
CCCAGGCCTCCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTAT	5	0.125	No Hit
AACTAATCATCTATTGCTTATTTAGGTCTTTCTCTCCTGCCAGAATGTGC	5	0.125	No Hit
GGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGA	5	0.125	No Hit
CCTGCACTCTCAGTAGGGACAGGCCCTATGCTGCCACCTGTACATGCTATCTGAAGGACAGCCTCCAGGGCACAC	5	0.125	No Hit
CCTCCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGC	5	0.125	No Hit
GACTGTGTGTGGGGGCCTGGGCACTGACTTCTGCAACCACCTGAGCGCGG	5	0.125	No Hit
CATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGGAGGG	5	0.125	No Hit
TGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCT	5	0.125	No Hit
GTCCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGC	5	0.125	No Hit
CGTGCAGCAGACAATGTGGCCTCTGCAGAGGGGGAACGGAGACCGGAGGC	5	0.125	No Hit
TCCCGCTGTCCTGAGCCCATGTTCCTCTCCCACATCATCAGGGGCACAGC	5	0.125	No Hit
TAGGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCA	5	0.125	No Hit
CTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGG	5	0.125	No Hit
CGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCA	5	0.125	No Hit
CCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGGAGTACC	5	0.125	No Hit
GGGGGTTGGAGGAGAGAACATATAGGAAAAATCAGAGCCAAATGAACCAC	5	0.125	No Hit
CCGTGCCCTAAAGGGTCTGCCCTGATTACTCCTGGCTCCTTGTGTGCAGGGGGCTCAGGCATGGCAGGGCTGGG	5	0.125	No Hit
GGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCC	5	0.125	No Hit
CCGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCC	5	0.125	No Hit
GTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCTGGGTGTC	5	0.125	No Hit
GGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCG	5	0.125	No Hit
GCCATCCAGGGGGCCTCTACAAGGATAATCTGACCTGCAGGGTCGAGGAG	5	0.125	No Hit
ATCCAGGGGGCCTCTACAAGGATAATCTGACCTGCAGGGTCGAGGAGTTGACGGTGCTGAGTTCCCTGCACTCTC	5	0.125	No Hit
CCGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTCCTCCGGGC	5	0.125	No Hit
CACATCATCAGGGGCACAGCGTGCACTGTGGGGTCCCAGGCCTCCCGAGC	5	0.125	No Hit
GGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAG	5	0.125	No Hit
CTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGA	5	0.125	No Hit
CTCAATGGCCACTCCGTGCTCCAGCCATGCTGGCTTCCTTTTAGGTCCTC	5	0.125	No Hit
GCACCACAGAAAACAGAGCCACCACAGAAAACAGAGGGTGACTGTCATCC	5	0.125	No Hit
CTCCGTGCTCCAGCCATGCTGGCTTCCTTTTAGGTCCTCCACCTCCAGGCTGTAGTTCATGTGCTTCTTTCTGGA	5	0.125	No Hit
ATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGC	5	0.125	No Hit
GGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAG	5	0.125	No Hit
CAGCAGGATTTTGACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGAGAACATAT	5	0.125	No Hit
CGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACCTCGGCTC	5	0.125	No Hit
CCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGC	5	0.125	No Hit
GTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGG	5	0.125	No Hit
CACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGA	5	0.125	No Hit
GTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTC	5	0.125	No Hit
CTTTATTAAAAGCACACTGTTGGTTTCTGCTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTT	5	0.125	No Hit
AGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCT	5	0.125	No Hit
GGGGAACCCAGAGCCTCACTTGTTCAGGCTCCCTCTGCCCTAGAAGTGAGAAGTCCAGAGCTCTACAGTTTGAAA	5	0.125	No Hit
CTTGTCTTTACCTCTGTGGGCAGCTCCCTTGTGATCTGCTTAGTTCCCACCCCCCTTTAAGAATTCAATAGAGA	5	0.125	No Hit
CACCACAGAAAACAGAGGGTGACTGTCATCCCCTCCAGTCTCTGCACACTCCCAGCTGCAGCAGAGCAGGAGG	5	0.125	No Hit
GGTCTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAA	5	0.125	No Hit
CAGAAAACAGAGCCACCACAGAAAACAGAGGGTGACTGTCATCCCCTCCAGTCTCTGCACACTCCCAGCTGCAGC	5	0.125	No Hit
CCTTGATCTTCTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATG	5	0.125	No Hit
TCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAG	5	0.125	No Hit
CCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTGG	5	0.125	No Hit
GTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTCCGCCAACC	5	0.125	No Hit
CCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCC	5	0.125	No Hit
GGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCT	5	0.125	No Hit
GGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCAGCTGTGTGGCCTC	5	0.125	No Hit
CGCCAACCTCGGCTCCTCCGGGCAGCCCTCGCCCGGGGTGCGCCCCGGGG	5	0.125	No Hit
GCCCTGGAGCAGGGTACTTGGCACTGGAGAACACCTTGATGGCCTTCTTG	5	0.125	No Hit
CTGGGAGACACAGCAGTGAAGCTGAAATGAAAAATGTGTTGCTGTAGTTT	5	0.125	No Hit
GTCTGTAGCCTCACCCCTGTCGTATCCTGACTACAATAACAGCTTCTGGG	5	0.125	No Hit
CGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTG	5	0.125	No Hit
CTTGAGAGGCTCGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGG	5	0.125	No Hit
CTTCAGCTTCTCCTGCAGGGCCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCT	5	0.125	No Hit
CTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGT	5	0.125	No Hit
GCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGG	5	0.125	No Hit
CTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAA	5	0.125	No Hit
GGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTCCCCGCCCCA	5	0.125	No Hit
CTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGT	5	0.125	No Hit
CGTCCTTTGTCCCATCGCTGCCTTCCCCTCAAGCTCAGGGCCAAGCTGTC	5	0.125	No Hit
CTCCAGCTCGCCTCCCTCAATGGCCACTCCGTGCTCCAGCCATGCTGGCT	5	0.125	No Hit
GTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
ERR5303525 read2 length is 28-76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5303525_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	28-76
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.9615	38.0	32.0	38.0	32.0	38.0
2	35.7525	38.0	32.0	38.0	32.0	38.0
3	35.7575	38.0	32.0	38.0	32.0	38.0
4	35.859	38.0	32.0	38.0	32.0	38.0
5	35.9265	38.0	32.0	38.0	32.0	38.0
6	38.191	40.0	38.0	40.0	38.0	40.0
7	38.27225	40.0	38.0	40.0	38.0	40.0
8	38.2975	40.0	38.0	40.0	38.0	40.0
9	38.43175	40.0	38.0	40.0	38.0	40.0
10-11	38.35225	40.0	38.0	40.0	38.0	40.0
12-13	38.3705	40.0	38.0	40.0	38.0	40.0
14-15	38.382875	40.0	38.0	40.0	38.0	40.0
16-17	38.391375	40.0	38.0	40.0	38.0	40.0
18-19	38.34125	40.0	38.0	40.0	38.0	40.0
20-21	38.250375000000005	40.0	38.0	40.0	38.0	40.0
22-23	38.3715	40.0	38.0	40.0	38.0	40.0
24-25	38.340625	40.0	38.0	40.0	38.0	40.0
26-27	38.43475	40.0	38.0	40.0	38.0	40.0
28-29	38.27191035258815	40.0	38.0	40.0	38.0	40.0
30-31	38.31957989497374	40.0	38.0	40.0	38.0	40.0
32-33	38.38809702425607	40.0	38.0	40.0	38.0	40.0
34-35	38.25156289072268	40.0	38.0	40.0	38.0	40.0
36-37	38.26844211052763	40.0	38.0	40.0	38.0	40.0
38-39	38.292823205801454	40.0	38.0	40.0	38.0	40.0
40-41	38.277569392348084	40.0	38.0	40.0	38.0	40.0
42-43	38.30132533133283	40.0	38.0	40.0	38.0	40.0
44-45	38.1609152288072	40.0	38.0	40.0	38.0	40.0
46-47	38.242060515128784	40.0	38.0	40.0	38.0	40.0
48-49	38.20055013753438	40.0	38.0	40.0	38.0	40.0
50-51	38.23868467116779	40.0	38.0	40.0	38.0	40.0
52-53	38.253813453363335	40.0	38.0	40.0	38.0	40.0
54-55	38.1565391347837	40.0	38.0	40.0	38.0	40.0
56-57	38.106526631657914	40.0	38.0	40.0	38.0	40.0
58-59	38.096649162290575	40.0	38.0	40.0	38.0	40.0
60-61	38.16999105006594	40.0	38.0	40.0	38.0	40.0
62-63	38.02239179384539	40.0	38.0	40.0	38.0	40.0
64-65	38.00739332501634	39.0	38.0	40.0	38.0	40.0
66-67	38.06738914936218	40.0	38.0	40.0	38.0	40.0
68-69	38.05233603537401	38.0	38.0	40.0	38.0	40.0
70-71	38.0223174424399	38.0	38.0	40.0	38.0	40.0
72-73	38.10321883192104	38.0	38.0	40.0	38.0	40.0
74-75	38.18037461640901	39.0	38.0	40.0	38.0	40.0
76	37.74623406720742	38.0	38.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	2.0
23	7.0
24	7.0
25	5.0
26	11.0
27	17.0
28	16.0
29	26.0
30	23.0
31	25.0
32	47.0
33	65.0
34	78.0
35	94.0
36	187.0
37	357.0
38	1209.0
39	1819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.05	16.0	7.6499999999999995	45.300000000000004
2	31.075000000000003	20.674999999999997	24.525	23.724999999999998
3	29.275000000000002	20.150000000000002	21.95	28.625
4	32.1	27.025	18.3	22.575
5	37.75	23.674999999999997	17.349999999999998	21.224999999999998
6	27.875	31.525	16.8	23.799999999999997
7	27.925	15.0	30.0	27.075
8	28.925	19.8	21.725	29.549999999999997
9	28.125	19.05	25.374999999999996	27.450000000000003
10-11	31.5125	22.975	19.9125	25.6
12-13	30.925000000000004	17.9	19.9375	31.2375
14-15	29.7375	20.8875	20.1375	29.2375
16-17	30.841355169396174	19.70246280785098	21.515189398674835	27.94099262407801
18-19	31.275	19.325	19.775000000000002	29.625
20-21	31.424999999999997	18.5625	20.9375	29.075
22-23	30.553819227403427	19.62745343167896	20.577572196524567	29.241155144393048
24-25	30.441305163145394	20.877609701212652	18.514814351793973	30.16627078384798
26-27	30.95	19.2625	19.6375	30.15
28-29	34.05425678209777	17.689711213901738	20.06500812601575	28.19102387798475
30-31	30.682670667666915	19.99249812453113	18.842210552638157	30.48262065516379
32-33	29.719929982495625	20.280070017504375	20.005001250312578	29.994998749687422
34-35	30.282570642660666	21.80545136284071	18.879719929982496	29.03225806451613
36-37	28.93223305826457	19.904976244061015	19.004751187796952	32.15803950987747
38-39	31.732933233308323	20.580145036259065	17.90447611902976	29.782445611402853
40-41	29.882470617654416	21.305326331582897	19.342335583895974	29.469867466866717
42-43	32.24556139034759	19.017254313578395	19.529882470617654	29.207301825456366
44-45	32.04551137784446	20.717679419854964	20.492623155788948	26.744186046511626
46-47	31.46179817431537	20.70776541202951	18.181818181818183	29.648618231836938
48-49	30.5114417906715	21.14542953607603	18.682005752157057	29.661122921095412
50-51	31.220305076269067	21.192798199549888	19.367341835458866	28.219554888722183
52-53	30.145036259064767	22.005501375343837	19.72993248312078	28.119529882470616
54-55	30.582645661415352	21.355338834708675	19.317329332333085	28.744686171542888
56-57	26.969242310577645	21.680420105026258	20.155038759689923	31.195298824706175
58-59	28.301650825412704	20.522761380690348	20.572786393196598	30.60280140070035
60-61	29.622216662496875	21.253440080060045	19.289467100325243	29.83487615711784
62-63	30.03003003003003	20.983483483483482	21.546546546546548	27.43993993993994
64-65	31.2875751503006	20.22795591182365	20.97945891783567	27.505010020040082
66-67	32.17020208359483	21.7145726120246	18.30048951926698	27.81473578511359
68-69	32.9685534591195	21.38364779874214	17.91194968553459	27.735849056603772
70-71	31.77004538577912	19.641956631366618	19.0494200706001	29.538577912254162
72-73	32.76843303402049	19.90641203996459	19.109649677500947	28.21550524851397
74-75	32.644343392586464	18.754306187129668	19.691332506545404	28.91001791373846
76	31.711085361143297	0.0	24.874468906913865	43.414445731942834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	3.5
27	7.5
28	11.0
29	13.5
30	9.0
31	5.0
32	2.5
33	1.0
34	3.5
35	6.0
36	5.0
37	6.0
38	16.0
39	17.5
40	16.5
41	22.0
42	30.0
43	34.0
44	35.5
45	54.0
46	66.5
47	54.5
48	59.0
49	98.0
50	116.0
51	107.0
52	103.0
53	160.0
54	223.0
55	230.5
56	267.5
57	236.5
58	167.0
59	222.0
60	283.0
61	233.0
62	166.5
63	194.0
64	252.0
65	230.0
66	227.0
67	252.5
68	212.0
69	140.5
70	96.5
71	102.5
72	96.0
73	69.5
74	41.0
75	14.5
76	6.0
77	12.0
78	15.5
79	12.5
80	9.5
81	14.0
82	17.0
83	11.5
84	12.5
85	16.5
86	11.5
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0125
18-19	0.0
20-21	0.0
22-23	0.0125
24-25	0.0125
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.012503125781445362
48-49	0.012503125781445362
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.025006251562890724
60-61	0.012507817385866166
62-63	0.02501876407305479
64-65	0.012523481527864746
66-67	0.025097251850922327
68-69	0.012577034335303737
70-71	0.012605571662674902
72-73	0.0
74-75	0.013777900248002206
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
28	1.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	1.0
60	1.0
61	0.0
62	0.0
63	3.0
64	3.0
65	3.0
66	7.0
67	4.0
68	3.0
69	6.0
70	3.0
71	7.0
72	9.0
73	111.0
74	418.0
75	831.0
76	2589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.9406392694064	22.75
2	21.404109589041095	18.75
3	10.787671232876713	14.174999999999999
4	5.993150684931506	10.5
5	3.4246575342465753	7.5
6	1.7123287671232876	4.5
7	1.3127853881278537	4.025
8	0.8561643835616438	3.0
9	0.5707762557077625	2.25
>10	1.9977168949771689	12.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCT	30	0.75	No Hit
CGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTT	25	0.625	No Hit
CAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGA	22	0.5499999999999999	No Hit
CGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCAC	21	0.525	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	20	0.5	No Hit
GGAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATG	18	0.44999999999999996	No Hit
CTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCT	18	0.44999999999999996	No Hit
CCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTC	17	0.42500000000000004	No Hit
CTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAAC	16	0.4	No Hit
CAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCA	16	0.4	No Hit
CTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGGAATCGTAGGGGGCTCCATGAC	16	0.4	No Hit
AGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGA	15	0.375	No Hit
CAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCA	15	0.375	No Hit
CGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	15	0.375	No Hit
CAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAG	14	0.35000000000000003	No Hit
CTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCC	14	0.35000000000000003	No Hit
CAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGC	13	0.325	No Hit
ATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTA	13	0.325	No Hit
GTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTA	13	0.325	No Hit
GTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCA	12	0.3	No Hit
GGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCA	12	0.3	No Hit
GGCGTGGGCTGGGGGTCCTGCCCCGGGGCGCACCCCGGGCGAGGGCTGCC	11	0.27499999999999997	No Hit
CTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGA	11	0.27499999999999997	No Hit
CCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCA	11	0.27499999999999997	No Hit
TGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACC	11	0.27499999999999997	No Hit
ACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGG	11	0.27499999999999997	No Hit
CAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGA	11	0.27499999999999997	No Hit
CCTGGAGGCTGTCCTTCAGATAGCATGTACAGGTGGCAGCATAGGGCCTGTCCCTACTGAGAGTGCAGGGAACTC	11	0.27499999999999997	No Hit
CTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGG	10	0.25	No Hit
CCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGG	10	0.25	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	10	0.25	No Hit
CCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACA	10	0.25	No Hit
GGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAG	10	0.25	No Hit
CTGTGATTGGATTTTGAGCAGGGCAGGGATGGGACAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGA	10	0.25	No Hit
AGGGCATCAGACACAGGTACAGCACATAGGCCAGGAGCCAGGGGGTGACGGGTGGCTCGGCTCGGGAGGCCTGGG	10	0.25	No Hit
CTTCAAGTTGATTCAGGCGTGTTAGGGAGCTGTGATTGGATTTTGAGCAG	9	0.22499999999999998	No Hit
CTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCTCCATG	9	0.22499999999999998	No Hit
CATCTGGCTTCCCTGTGTGGGAGGGGCTGAAGTGAGAGCCCAACTTGGAA	9	0.22499999999999998	No Hit
GTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTC	9	0.22499999999999998	No Hit
CCAAGAGAGTGCACGGTCCCTGCTGATTGAAAGAAGGATGAAGGGCAGAAGAGGGGCGGGGAGCTGTGTGCCCTA	9	0.22499999999999998	No Hit
CCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCT	9	0.22499999999999998	No Hit
CAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGG	9	0.22499999999999998	No Hit
CACGGATTACTCAAGACAGGACTTCAAGTTGATTCAGGCGTGTTAGGGAGCTGTGATTGGATTTTGAGCAGGGC	9	0.22499999999999998	No Hit
GCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGC	9	0.22499999999999998	No Hit
TGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACG	9	0.22499999999999998	No Hit
CCGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCT	8	0.2	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	8	0.2	No Hit
AGCAGATGGCGGATGCCCTGCAGTACCTGCAGAAGGTCTCTGGAGACATC	8	0.2	No Hit
GTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTC	8	0.2	No Hit
CTCCAAGAGAGTGCACGGTCCCTGCTGATTGAAAGAAGGATGAAGGGCAG	8	0.2	No Hit
CGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCT	8	0.2	No Hit
CAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAG	8	0.2	No Hit
CCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTG	8	0.2	No Hit
AGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGA	8	0.2	No Hit
TGCAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAG	8	0.2	No Hit
CTCTGTTTTCTGTGGTGCTGGATGCACCTCTGTTTTCTGTGGTGGCTCCA	8	0.2	No Hit
GGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAG	8	0.2	No Hit
CAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCA	8	0.2	No Hit
GGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACC	8	0.2	No Hit
CTCTGCCAGATCCCTCCGTGCAAGGCTGGAATCAGTCCATTGTTCAACTG	8	0.2	No Hit
CTGGCCTGCAGAGACGCTCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGA	7	0.17500000000000002	No Hit
ATCTGGCTTCCCTGTGTGGGAGGGGCTGAAGTGAGAGCCCAACTTGGAAG	7	0.17500000000000002	No Hit
CGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCC	7	0.17500000000000002	No Hit
GGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGA	7	0.17500000000000002	No Hit
CTTGGCCCTGAGCTTGAGGGGAAGGCAGCGATGGGACAAAGGACGGAGGTCTAGGAAGAGGGTCTGCAGAGCAGA	7	0.17500000000000002	No Hit
GGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCC	7	0.17500000000000002	No Hit
CCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGC	7	0.17500000000000002	No Hit
GCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCA	7	0.17500000000000002	No Hit
GACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGG	7	0.17500000000000002	No Hit
AGGCAGGTGAGCTCCTGGCAAATTAGCATTGCAGGCTGTGCTCTCTCCTC	7	0.17500000000000002	No Hit
ACCTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCT	7	0.17500000000000002	No Hit
CGGCATTGCCCCCTCTGCCCCTGGCACCATTCCAGAACTGCCCACCTTCCACACTGAGGTAGCCGAGCCTCTCA	7	0.17500000000000002	No Hit
GGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGC	7	0.17500000000000002	No Hit
AGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGG	7	0.17500000000000002	No Hit
CAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGCAGCTGGAACAGCAGGTCCC	7	0.17500000000000002	No Hit
GCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	7	0.17500000000000002	No Hit
GGAGGCCGTCCAGCAGATGGCGGATGCCCTGCAGTACCTGCAGAAGGTCT	7	0.17500000000000002	No Hit
CATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACCATTCCAGAACTGCCCACCTTCCACACTG	7	0.17500000000000002	No Hit
TGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCA	7	0.17500000000000002	No Hit
CAAGCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGG	7	0.17500000000000002	No Hit
CCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAG	7	0.17500000000000002	No Hit
GCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGC	7	0.17500000000000002	No Hit
GGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGC	7	0.17500000000000002	No Hit
CCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCC	6	0.15	No Hit
GGCAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCCTGCCCCGGGGCGCAC	6	0.15	No Hit
GTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCT	6	0.15	No Hit
CCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAGACCAGCTTCAAGGAGCGGAAGGCTGGCTTG	6	0.15	No Hit
CGAGAACCTGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTG	6	0.15	No Hit
AGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGAC	6	0.15	No Hit
CCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGG	6	0.15	No Hit
AGTAGATGTTTGCAGACAGAAAGTAAACCACTCATCAGGTGTATTCAGTC	6	0.15	No Hit
CCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCAT	6	0.15	No Hit
GGTTGAGTGGGTAGGTTGGGAAGAACATTCCAGAAAGAAGCACATGAACTACAGCCTGGAGGTGGAGGACCTAAA	6	0.15	No Hit
CCTGCCCGGCATTGCCAACGACCTCATGTACATTGCCGACCTGGGCCCCG	6	0.15	No Hit
ATTCGATCATGAAACCAGCTTACCCTTCCCCTGTGTGCTGGCCCCAGTTTTCTAACCAGGTGTTGAATGAACTGG	6	0.15	No Hit
GTGGGGAGCACCGCCACCTCTCCTCCTCCACTTGCCCCTCCTGTGGCACT	6	0.15	No Hit
ATGACACCTTCCCCGCCAGACCCAGACTTGGGCCGTTGCTCTGACATGGA	6	0.15	No Hit
CTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCA	6	0.15	No Hit
CCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCC	6	0.15	No Hit
CCCAGTTTTCTAACCAGGTGTTGAATGAACTGGATGGACTCTGCCAGATC	6	0.15	No Hit
CAGAAGAGTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGG	6	0.15	No Hit
GTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCT	6	0.15	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTAGA	6	0.15	No Hit
GCCCCGGGGCGCACCCCGGGCGAGGGCTGCCCGGAGGAGCCGAGGTTGGC	6	0.15	No Hit
CAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTAGA	6	0.15	No Hit
AGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATG	6	0.15	No Hit
ACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	6	0.15	No Hit
GGGGGTGACGGGTGGCTCGGCTCGGGAGGCCTGGGACCCCACAGTGCACG	6	0.15	No Hit
CAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGG	6	0.15	No Hit
GCCTGAGATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCA	6	0.15	No Hit
GGCGTAGGGCTGGAGGGCAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCC	6	0.15	No Hit
TATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCC	6	0.15	No Hit
CAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATCTTCACGGGCGCCC	6	0.15	No Hit
CGACTGGCAGTGGAGTGGGGAGCACCGCCACCTCTCCTCCTCCACTTGCC	5	0.125	No Hit
GGGGAGCACCGCCACCTCTCCTCCTCCACTTGCCCCTCCTGTGGCACTGG	5	0.125	No Hit
CCCAGTGTAACCCTAGCATCCAGAAGTGGCACAAAACCCCTCTGCTGGCTCGTGTGTGCAACTGAGACTGTCAG	5	0.125	No Hit
CATGGACACAGCCAGGACAAGCTGCTCAGACCTACTTCCTTGGGAGGGGG	5	0.125	No Hit
GCTCGTGTGCAAGGCAGTGTGGGAATCTCTATATATCCAGCCACGGATAA	5	0.125	No Hit
CTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCC	5	0.125	No Hit
GTGAGTTAGGGCCCACGGATTACTCAAGACAGGACTTCAAGTTGATTCAG	5	0.125	No Hit
AGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGAC	5	0.125	No Hit
GGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	5	0.125	No Hit
GACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	5	0.125	No Hit
CTGGAATCAGTCCATTGTTCAACTGTGCCCTTTGGGGCTGTGGTTCATTT	5	0.125	No Hit
GACATCTTCAGCAGGTAGAGCAGAGCCGGAGCCAGGTGCAGGCCATTGGAGAGAAGGTCTCCTTGGCCCAGGCCA	5	0.125	No Hit
CCATGACACCTTCCCCCCCAGACCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCTC	5	0.125	No Hit
GGCGGATGCCCTGCAGTACCTGCAGAAGGTCTCTGGAGACATCTTCAGCA	5	0.125	No Hit
TTCAACTGTGCCCTTTGGGGCTGTGGTTCATTTGGCTCTGATTTTTCCTATATGTTCTCTCCTCCAACCCCCAT	5	0.125	No Hit
GGCGTGTTAGGGAGCTGTGATTGGATTTTGAGCAGGGCAGGGATGGGACA	5	0.125	No Hit
GGATGACAGTCACCCTCTGTTTTCTGTGGTGGCTCTGTTTTCTGTGGTGC	5	0.125	No Hit
GCAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGA	5	0.125	No Hit
GCTGCTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGT	5	0.125	No Hit
CGTGTGCAAGGCAGTGTGGGAATCTCTATATATCCAGCCACGGATAAGGC	5	0.125	No Hit
CGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGGACACCCAGAAGC	5	0.125	No Hit
CCTTTACAGCAGATGAAGGCCTCTCCCCAGCCAGAAAAGATGGAGCACAC	5	0.125	No Hit
AGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCTCCATGGGGG	5	0.125	No Hit
CACCCCGGGCGAGGGCTGCCCGGAGGAGCCGAGGTTGGCGGACAGCTTGGCCCTGAGCTTGAGGGGAAGGCAGCG	5	0.125	No Hit
GCTCCTGGCAAATTAGCATTGCAGGCTGTGCTCTCTCCTCCTGCTCTGCTGCAGCTGGGAGTGTGCAGAGACTGG	5	0.125	No Hit
CCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGA	5	0.125	No Hit
GGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGGGACTTCTGTCTGCCT	5	0.125	No Hit
GGGCAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCCTGCCCCGGGGCGCACCCCGGGCGAGGGCTGCCCGGAGG	5	0.125	No Hit
GGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAG	5	0.125	No Hit
GGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTT	5	0.125	No Hit
CAGGAAAGCGGGTCAAGGCGTAGGGCTGGAGGGCAGGGGCGGGCCCTGGG	5	0.125	No Hit
GGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCA	5	0.125	No Hit
CAGCTGGGGCGGGGACTTCTGTCTGCCTGTGCTCCATGGGGGGACGGCTC	5	0.125	No Hit
CTGGAGGGGATGACAGTCACCCTCTGTTTTCTGTGGTGGCTCTGTTTTCT	5	0.125	No Hit
AGAGCAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCA	5	0.125	No Hit
GGTGGCTCTGTTTTCTGTGGTGCTGGATGCACCTCTGTTTTCTGTGGTGG	5	0.125	No Hit
ATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTGCCCCTGGCACCATTCC	5	0.125	No Hit
GACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGC	5	0.125	No Hit
GGCTGGAGGGCAGGGGCGGGCCCTGGGCGTGGGCTGGGGGTCCTGCCCCG	5	0.125	No Hit
CTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGC	5	0.125	No Hit
GAGGAAATGACATTTATGGTAAAGTCTGAGGGTTGAGTGGGTAGGTTGGG	5	0.125	No Hit
GAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCT	5	0.125	No Hit
CACCAGAGGTCCTCAGCCCTCCTGCCTGGGCTATGGCTCCTTCCTGGTTT	5	0.125	No Hit
GATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGCAGCTGGAAC	5	0.125	No Hit
CGCCACCTCTCCTCCTCCACTTGCCCCTCCTGTGGCACTGGACAAGCTAG	5	0.125	No Hit
CAGAAAGTAAACCACTCATCAGGTGTATTCAGTCCCATTCTTGAACGGGC	5	0.125	No Hit
GGGCAGTTCCCCGTCTGAATTTAGGTGACGACACTCAGGTCCAGCCTTGC	5	0.125	No Hit
CGAGGACGACTGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTGGGCCGTTGCTCTGAC	5	0.125	No Hit
GCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCG	5	0.125	No Hit
CAGGGGGTGACGGGTGGCTCGGCTCGGGAGGCCTGGGACCCCACAGTGCACGCTGTGCCCCTGATGATGTGGG	5	0.125	No Hit
CCTTCAGATAGCATGTACAGGTGGCAGCATAGGGCCTGTCCCTACTGAGA	5	0.125	No Hit
GATTGATGTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACC	5	0.125	No Hit
AGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCT	5	0.125	No Hit
TCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGGACGACTGGG	5	0.125	No Hit
CAGTTTTCTAACCAGGTGTTGAATGAACTGGATGGACTCTGCCAGATCCC	5	0.125	No Hit
GTTTGGGGAAGGTTCCTCAGGCATCCGTCACGGAAGGGACAAGAAGGGAGAGAGAGTGGATGCCAGGGACACCC	5	0.125	No Hit
TGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGAC	5	0.125	No Hit
CCCGGTCCTGGGGAACCCATCTGGCTTCCCTGTGTGGGAGGGGCTGAAGT	5	0.125	No Hit
ATTGGATTTTGAGCAGGGCAGGGATGGGACAGAAGAGTTTGGGGAAGGTT	5	0.125	No Hit
AGCTGAAGGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
64	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
Read 3251666 spots for ERR5303525.sra
Written 3251666 spots for ERR5303525.sra
2025-05-07T13:17:15 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open KI270717.1 as a RefSeq or as a WGS
2025-05-07T13:17:46 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open KI270728.1 as a RefSeq or as a WGS
2025-05-07T13:17:57 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open KI270734.1 as a RefSeq or as a WGS
2025-05-07T13:18:17 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed ERR5303525.sra

=============================================================
An error occurred during processing.
A report was generated into the file '/home/mark.ziemann@domain.internal.burnet.edu.au/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
SRR ids: ['ERR5303525.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_dh1ce0l7
ERR5303525.sra spots: 52026657
blocks: [[1, 3251666], [3251667, 6503332], [6503333, 9754998], [9754999, 13006664], [13006665, 16258330], [16258331, 19509996], [19509997, 22761662], [22761663, 26013328], [26013329, 29264994], [29264995, 32516660], [32516661, 35768326], [35768327, 39019992], [39019993, 42271658], [42271659, 45523324], [45523325, 48774990], [48774991, 52026657]]
fastq-dump error! exit code: 3
ERR5303525 file size 9260596
ERR5303525 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 16 -o ERR5303525 ERR5303525_1.fastq ERR5303525_2.fastq
Input file:	ERR5303525_1.fastq
Paired file:	ERR5303525_2.fastq
trimmed:	ERR5303525-trimmed-pair1.fastq, ERR5303525-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Wed May  7 13:20:00 2025 >> started

Wed May  7 13:20:43 2025 >> done (42.840s)
48774990 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
48774990 (100.00%) read pairs available; of these:
    2646 ( 0.01%) trimmed read pairs available after processing
48772344 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 25	      48	  0.00%
 26	      57	  0.00%
 27	      95	  0.00%
 28	     123	  0.00%
 29	     154	  0.00%
 30	     205	  0.00%
 31	     205	  0.00%
 32	     312	  0.00%
 33	     340	  0.00%
 34	     390	  0.00%
 35	     426	  0.00%
 36	     495	  0.00%
 37	     579	  0.00%
 38	     694	  0.00%
 39	     798	  0.00%
 40	     916	  0.00%
 41	    1162	  0.00%
 42	    1160	  0.00%
 43	    1338	  0.00%
 44	    1383	  0.00%
 45	    1509	  0.00%
 46	    1675	  0.00%
 47	    2031	  0.00%
 48	    2480	  0.01%
 49	    2930	  0.01%
 50	    3866	  0.01%
 51	    5461	  0.01%
 52	    5569	  0.01%
 53	    5816	  0.01%
 54	    6631	  0.01%
 55	    7069	  0.01%
 56	    7638	  0.02%
 57	    7973	  0.02%
 58	    9218	  0.02%
 59	   10626	  0.02%
 60	   11989	  0.02%
 61	   13794	  0.03%
 62	   15412	  0.03%
 63	   17773	  0.04%
 64	   20139	  0.04%
 65	   22842	  0.05%
 66	   25577	  0.05%
 67	   28289	  0.06%
 68	   32195	  0.07%
 69	   38781	  0.08%
 70	   49248	  0.10%
 71	   67645	  0.14%
 72	  117503	  0.24%
 73	  547594	  1.12%
 74	 4322485	  8.86%
 75	22764958	 46.67%
 76	20587394	 42.21%
48774990 reads passed initial QC


criterion=sequence-density
sequence-density=0.24
sequence-density-rank=1
fanout-score=9.72
fanout-score-rank=13
prefix-density=0.76
prefix-fanout=3.1
sequence=CTTCTTCTTCTTGGGGGTCTCCTTGGGGGGGACCTCCCCCTTGGCCTCAGCACCCTGGCTAGGGGGTGCTGGCTCGATGGCATCGCCAGTGGCCCCGGCTGCCTCATCTGTTCCGTTCACAGGGGGCGACTCCCCTTCACCCTTGGGGGATAAGTCTCCATTGCTTTTCACGTGGCCATTCTCCTGGCCGTTGGCCTTCGCGGGGGAAGCGCCTGCTGCCTCCTCGGCGGTCACGTCGCCCCGGGGAGCCTTGGAGCTCTGGCTGCCCATGATGGGGGTCTGCTGGGGGGCGCTTGGAGCCGCCCCGGGCTCGCGCCGCAGGGGATAGTACGGCGGGGTCGGCCCGGCCGGCGGAGGGGTGGGGCTGGCGCCCGAGGGGGAGGGGTGCCGGGGGAAGCCGAGCTGCACCTCCGCTCCGCGGCCGACCCGCT


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=12
fanout-score=70.83
fanout-score-rank=1
prefix-density=0.46
prefix-fanout=12.1
sequence=CCTCCTCCTCCTGCTCCTGCCGGGAATACACAACAGGAGGCACCTCGAGGACCTTGTCCACATTCTTCA


criterion=sequence-density
sequence-density=0.45
sequence-density-rank=1
fanout-score=4.81
fanout-score-rank=29
prefix-density=0.64
prefix-fanout=3.4
sequence=AGGCCAAGGGGGCAGAGGCTAGTGCAGCCTCAGAAGAAGAGGCAGGGCCCCAGGCTACAGAGCCATCCACTCCCTCGGGGCCGGAGAGTGGCCCTACACCAGCCAGCGCTGAGCAGAATGAGTAGCTAGGTAGGGGCAGGTGGGTGATCTCTAAGCTGCAAAAACTGTGCTGTCCTTGTGAGGTCACTGCCTGGACCTGGTGCCCTGGCTGCCTTCCTGTGCCCAGAAAGGAAGGGGCTATTGCCTCCTCCCAGCCACGTTCCCTTTCCTCCTCTCCCTCCTGTGGATTCTCCCATCAGCCATCTGGTTCTCCTCTTAAGGCCAGTTGAAGATGGTCCCTTACAGCTTCCCAAGTTAGGTTAGTGATGTGAAATGCTCCTGTCCCTGGCCCTACCTCCTTCCCTGTCCCCACCCCTGCATAAGGCAGTTGTTGGTTTTCTTCCCCAATTCTTTTCCAAGTAGGTTTTGTTTACCCTACTCCCCAAATCCCTGAGCCAGAAGTGGGGTGCTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=66.01
fanout-score-rank=1
prefix-density=0.20
prefix-fanout=2.2
sequence=CAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGCAAGAGAAAGCCCTACCACAAGAAGCGGAAGTATGAGTTGGGGCGCCCAGCTGCCAACACCAAGATTGGCCCCCGCCGCATCCACACAGTCCGTGTGCGGGGAGGTAACAAGAAATACCGTGCCCTGAGGTTGGACGTGGGGAATTTCTCCTGGGGCTCAGAGTGTTGTACTCGTAAAACAAGGATCATCGATGTTGTCTACAATGCATCTAATAACGAGCTGGTTCGTACCAAGACCCTGGTGAAGAATTGCATCGTGCTCATCGACAGCACACCGTACCGACAGTGGTACGAGTCCCACTATGCGCTGCCCCTGGGCCGCAAGAAGGGAGCCAAGCTG
ERR5303525 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 07 13:21:09
                             Started mapping on |	May 07 13:21:10
                                    Finished on |	May 07 13:22:19
       Mapping speed, Million of reads per hour |	2544.78

                          Number of input reads |	48774990
                      Average input read length |	150
                                    UNIQUE READS:
                   Uniquely mapped reads number |	46917637
                        Uniquely mapped reads % |	96.19%
                          Average mapped length |	150.44
                       Number of splices: Total |	24293460
            Number of splices: Annotated (sjdb) |	24023760
                       Number of splices: GT/AG |	24069499
                       Number of splices: GC/AG |	175215
                       Number of splices: AT/AC |	24005
               Number of splices: Non-canonical |	24741
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.65
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.40
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1856614
             % of reads mapped to multiple loci |	3.81%
        Number of reads mapped to too many loci |	13
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.00%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	739	739	739
N_multimapping	1856614	1856614	1856614
N_noFeature	3665299	44405852	4036419
N_ambiguous	3806068	50670	1748112
UnstrandedReadsAssigned:39446270 PositiveStrandReadsAssigned:2461115 NegativeStrandReadsAssigned:41133106
Dataset is classified negative stranded
MeadianReadLen=76 20thPercentileLength=75 echo kmer=71
ERR5303525 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in paired-end mode
[quant] will process pair 1: ERR5303525-trimmed-pair1.fastq
                             ERR5303525-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 48,774,990 reads, 43,208,605 reads pseudoaligned
[quant] estimated average fragment length: 135.725
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,253 rounds

 180870 ERR5303525.ke.tsv
  58302 ERR5303525.se.tsv
 239172 total
==> ERR5303525.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	10	0	0
ENST00000448914.1	13	14	0	0
ENST00000415118.1	8	9	0	0
ENST00000631435.1	12	13	0	0
ENST00000632684.1	12	13	0	0
ENST00000454908.1	17	4	0	0
ENST00000390567.1	20	7	0	0
ENST00000603326.1	19	6	0	0
ENST00000414852.1	16	3	0	0

==> ERR5303525.se.tsv <==
ENSG00000223972	0
ENSG00000227232	56
ENSG00000278267	51
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	8
ERR5303525 completed mapping pipeline successfully
