Starting /dee2/code/volunteer_pipeline.sh ERR5296849
    current disk space = 1385343612
    free memory = 122646156 
ERR5296849 SRAfilesize
e345dbec1cda805974214afdbe4894ad  ERR5296849.sra
ERR5296849.sra file validated
ERR5296849 is paired end
ERR5296849 is conventional basespace
ERR5296849 read1 length is 75-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5296849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75-150
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3465	37.0	37.0	37.0	37.0	37.0
2	36.57125	37.0	37.0	37.0	37.0	37.0
3	36.58625	37.0	37.0	37.0	37.0	37.0
4	36.5845	37.0	37.0	37.0	37.0	37.0
5	36.5945	37.0	37.0	37.0	37.0	37.0
6	36.594	37.0	37.0	37.0	37.0	37.0
7	36.693	37.0	37.0	37.0	37.0	37.0
8	36.703	37.0	37.0	37.0	37.0	37.0
9	36.789	37.0	37.0	37.0	37.0	37.0
10-14	36.6795	37.0	37.0	37.0	37.0	37.0
15-19	36.693200000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.7001	37.0	37.0	37.0	37.0	37.0
25-29	36.6655	37.0	37.0	37.0	37.0	37.0
30-34	36.6575	37.0	37.0	37.0	37.0	37.0
35-39	36.6001	37.0	37.0	37.0	37.0	37.0
40-44	36.5822	37.0	37.0	37.0	37.0	37.0
45-49	36.5825	37.0	37.0	37.0	37.0	37.0
50-54	36.5088	37.0	37.0	37.0	37.0	37.0
55-59	36.524950000000004	37.0	37.0	37.0	37.0	37.0
60-64	36.5119	37.0	37.0	37.0	37.0	37.0
65-69	36.496700000000004	37.0	37.0	37.0	37.0	37.0
70-74	36.4938	37.0	37.0	37.0	37.0	37.0
75-79	36.487327532008095	37.0	37.0	37.0	37.0	37.0
80-84	36.487386861467414	37.0	37.0	37.0	37.0	37.0
85-89	36.46693366708385	37.0	37.0	37.0	37.0	37.0
90-94	36.40609972332684	37.0	37.0	37.0	37.0	37.0
95-99	36.40154707635829	37.0	37.0	37.0	37.0	37.0
100-104	36.34273255262854	37.0	37.0	37.0	37.0	37.0
105-109	36.30722963903465	37.0	37.0	37.0	37.0	37.0
110-114	36.29944144259326	37.0	37.0	37.0	37.0	37.0
115-119	36.31993716432825	37.0	37.0	37.0	37.0	37.0
120-124	36.286491554262774	37.0	37.0	37.0	37.0	37.0
125-129	36.25805695151239	37.0	37.0	37.0	37.0	37.0
130-134	36.254461003121094	37.0	37.0	37.0	37.0	37.0
135-139	36.19771803673841	37.0	37.0	37.0	37.0	37.0
140-144	36.14178635053736	37.0	37.0	37.0	37.0	37.0
145-149	36.099581199139955	37.0	37.0	37.0	37.0	37.0
150	35.96537360062484	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	3.0
25	1.0
26	2.0
27	2.0
28	6.0
29	15.0
30	23.0
31	17.0
32	39.0
33	47.0
34	74.0
35	199.0
36	2979.0
37	592.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.65	11.675	10.9	47.775
2	25.98149537384346	20.730182545636406	34.7586896724181	18.529632408102024
3	26.556639159789945	21.255313828457115	20.230057514378593	31.957989497374346
4	27.800000000000004	30.875000000000004	16.475	24.85
5	30.85	23.599999999999998	19.675	25.874999999999996
6	24.4	28.000000000000004	20.549999999999997	27.05
7	22.575	11.899999999999999	35.5	30.025000000000002
8	24.725	16.5	23.225	35.55
9	24.55	16.475	27.35	31.624999999999996
10-14	29.01	20.455000000000002	21.955	28.58
15-19	29.310000000000002	20.325	20.979999999999997	29.385
20-24	28.935	20.74	21.13	29.195
25-29	30.115	20.055	20.57	29.26
30-34	29.68	20.145	20.95	29.225
35-39	30.175	19.869999999999997	20.865000000000002	29.09
40-44	28.615000000000002	20.54	21.145	29.7
45-49	29.830000000000002	20.72	21.044999999999998	28.405
50-54	28.775000000000002	20.77	21.535	28.92
55-59	29.231461573078654	21.391069553477674	19.785989299464973	29.591479573978695
60-64	29.675	20.785	20.555	28.985
65-69	29.294999999999998	20.28	21.295	29.13
70-74	28.505000000000003	20.825	21.175	29.494999999999997
75-79	28.13844153245974	20.88626587976393	19.800940282084625	31.174352305691706
80-84	28.262610088070456	18.96517213771017	22.548038430744597	30.224179343474777
85-89	29.521902377972463	18.758448060075096	20.81602002503129	30.90362953692115
90-94	29.599959945927	20.292394732889402	20.63285435337706	29.47479096780654
95-99	31.084941117514408	19.849661738912552	21.197694813329992	27.86770233024305
100-104	29.40851576621812	19.863426390841536	20.66680056236192	30.06125728057843
105-109	29.314765471821424	21.01955658337942	20.71791262379971	28.947765320999448
110-114	29.39752961935972	19.284093773632467	22.102344340811698	29.21603226619612
115-119	29.76991150442478	20.18710493046776	20.895069532237674	29.14791403286979
120-124	29.35705209656925	20.025412960609913	20.75222363405337	29.865311308767474
125-129	29.692989155338324	20.88488366172802	19.998981721908255	29.423145461025406
130-134	29.921420553117667	21.083784059597917	20.28268190631697	28.712113480967442
135-139	30.374095550880075	20.849797300764614	20.03386873300149	28.742238415353828
140-144	29.865841073271415	20.546955624355004	20.03095975232198	29.556243550051597
145-149	28.854739902398507	20.828574395182223	20.558612812792028	29.758072889627247
150	28.06560791460557	22.780525904712317	21.81723509502734	27.336631085654776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	2.0
30	3.0
31	7.5
32	8.5
33	2.5
34	4.5
35	11.0
36	18.0
37	16.0
38	12.0
39	17.5
40	39.5
41	43.5
42	27.0
43	29.0
44	36.0
45	37.0
46	31.0
47	42.0
48	47.5
49	57.5
50	66.0
51	62.0
52	68.5
53	84.0
54	111.5
55	143.5
56	180.5
57	224.0
58	207.0
59	167.0
60	213.5
61	254.5
62	236.0
63	216.0
64	194.5
65	195.0
66	198.5
67	191.5
68	177.0
69	136.5
70	83.5
71	46.5
72	34.5
73	15.5
74	3.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.005
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
74-75	1.0
76-77	0.0
78-79	2.0
80-81	0.0
82-83	1.0
84-85	1.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.0
94-95	2.0
96-97	0.0
98-99	2.0
100-101	9.0
102-103	1.0
104-105	0.0
106-107	2.0
108-109	0.0
110-111	12.0
112-113	4.0
114-115	3.0
116-117	5.0
118-119	14.0
120-121	5.0
122-123	1.0
124-125	2.0
126-127	6.0
128-129	1.0
130-131	4.0
132-133	7.0
134-135	11.0
136-137	10.0
138-139	11.0
140-141	6.0
142-143	3.0
144-145	15.0
146-147	7.0
148-149	9.0
150-151	3841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.775000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.11369019422075	31.724999999999998
2	19.469445760303174	20.549999999999997
3	9.758408337280908	15.45
4	4.547607768829939	9.6
5	1.9422074846044528	5.125
6	1.6106110847939363	5.1
7	0.994789199431549	3.675
8	0.7105637138796779	3.0
9	0.28422548555187116	1.35
>10	0.5684509711037423	4.425
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTCCATCCTCCCTGGTGCGGGGTGGGCCCAGTGATATCAGCTGCCTGCT	21	0.525	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	21	0.525	No Hit
CGCAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGG	19	0.475	No Hit
GTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGC	17	0.42500000000000004	No Hit
CTAAGATCTCATTGCCTTTTTATGCCGATTAACATGCTTTTAGCCCCTAC	16	0.4	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCA	14	0.35000000000000003	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	13	0.325	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	13	0.325	No Hit
CACGAGCCAAGGTGGGCACTTGATGTCGGATCTCTTCAACAAGCTGGTCA	12	0.3	No Hit
CAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAG	11	0.27499999999999997	No Hit
GCCAGGTGCAGGCCATTGGAGAGAAGGTCTCCTTGGCCCAGGCCAAGATT	10	0.25	No Hit
AAGACCCCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGC	10	0.25	No Hit
CTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCA	9	0.22499999999999998	No Hit
CTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAG	9	0.22499999999999998	No Hit
CTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCT	9	0.22499999999999998	No Hit
CGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAG	9	0.22499999999999998	No Hit
GGAAGACCCCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGT	9	0.22499999999999998	No Hit
CTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAG	9	0.22499999999999998	No Hit
CAGCCTCAGAGGCACGGGGAAATGGAGGGACTGGGCAGCCTCAGAGGCAC	8	0.2	No Hit
CTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGG	8	0.2	No Hit
CTTGCTTTAGACACAGTTCAGGTGAAGAAAGACCTGGAAACTTCTGTTAA	8	0.2	No Hit
GTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGAATGGGGAAC	8	0.2	No Hit
CTCAGACAATTTTTAAAGGCCAGAGGGTAGACTGCAATCACCAAGATGAA	8	0.2	No Hit
CCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTACGATTCC	8	0.2	No Hit
GGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGG	8	0.2	No Hit
GTAAATTTCATCTTGGTGATTGCAGTCTACCCTCTGGCCTTTAAAAATTG	8	0.2	No Hit
AAAAAGGCAATGAGATCTTAGGGCACACAGCTCCCCGCCCCTCTTCTGCC	8	0.2	No Hit
TGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGC	8	0.2	No Hit
ATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGA	8	0.2	No Hit
CACAGGGGAAGGGTAAGCTGGTTTCATGATCGAATCAAGGCTCAGACAAT	8	0.2	No Hit
TGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTG	8	0.2	No Hit
GTCTTTCTTCACCTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGT	8	0.2	No Hit
CTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGA	8	0.2	No Hit
CAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAG	7	0.17500000000000002	No Hit
CTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCC	7	0.17500000000000002	No Hit
CGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAA	7	0.17500000000000002	No Hit
GTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGT	7	0.17500000000000002	No Hit
CCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGA	7	0.17500000000000002	No Hit
CCTTCCGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCC	7	0.17500000000000002	No Hit
CTGACTTCTGCAACCACCTGAGCGCGGGCATCCTGTGTGCAGATACTCCC	7	0.17500000000000002	No Hit
CATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACGAGG	7	0.17500000000000002	No Hit
GTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTTGCTGATGGACAAGG	7	0.17500000000000002	No Hit
GGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTC	7	0.17500000000000002	No Hit
CTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAG	7	0.17500000000000002	No Hit
GGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTT	7	0.17500000000000002	No Hit
CGCGCTCAGGTGGTTGCAGAAGTCAGTGCCCAGGCCCCCACACACAGTCT	7	0.17500000000000002	No Hit
CTGTCGTCCTGCCTGGCGCCTTGGCCTACAGGGGCCGCGGTTGAGGGTGG	7	0.17500000000000002	No Hit
GAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCG	7	0.17500000000000002	No Hit
CTTGATTCGATCATGAAACCAGCTTACCCTTCCCCTGTGTGCTGGCCCCA	7	0.17500000000000002	No Hit
CTTGTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGG	7	0.17500000000000002	No Hit
CTCGGCTCGGGAGGCCTGGGACCCCACAGTGCACGCTGTGCCCCTGATGA	7	0.17500000000000002	No Hit
CTTAAGTGTTCCATGACAGACTGGTATGAAGGTGGCCACAATTCAGAAAG	7	0.17500000000000002	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGC	7	0.17500000000000002	No Hit
GGAAGGGTAAGCTGGTTTCATGATCGAATCAAGGCTCAGACAATTTTTAA	7	0.17500000000000002	No Hit
CTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCA	6	0.15	No Hit
CCCAGCTTTCCCTAGCCCAGAGTCTGCAGCCCCTCCACCATCCCACATCC	6	0.15	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	6	0.15	No Hit
CTGATTTCTGGCGTGCTCAGTGCACTCGGGTGGATTTTCTGTGGGTTTGT	6	0.15	No Hit
GGAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATG	6	0.15	No Hit
ATTTATACGCTTGATTTTTAAAACCTAAATGTTGGGCTTCACATTTGTTC	6	0.15	No Hit
CCACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTC	6	0.15	No Hit
CGCCCGTGAAGATGGAGCCATATTCCTGCAGGCGCCCTGGAGCAGGGTAC	6	0.15	No Hit
CCACCAACCACCCTCACTCAGCCTTTTCCCTCCAGGCATCTCTGGGAAAG	6	0.15	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	6	0.15	No Hit
CAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGA	6	0.15	No Hit
GGCCATTGGAGAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCA	6	0.15	No Hit
GCCACTTCTGGATGCTAGGGTTACACTGGGAGACACAGCAGTGAAGCTGA	6	0.15	No Hit
CCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGC	6	0.15	No Hit
CTCAGGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTT	6	0.15	No Hit
GAGCAAGTGAGAGCCACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTT	6	0.15	No Hit
ATTTTTCATTTCAGCTTCACTGCTGTGTCTCCCAGTGTAACCCTAGCATC	6	0.15	No Hit
GTTTTCTGTGGTGCTGGATGCACCTCTGTTTTCTGTGGTGGCTCCAAGAG	6	0.15	No Hit
CCTCCTCCTGCCCTTCCTCATGAACCCCAGTCGCGCCTCTGCCTTCTCAT	6	0.15	No Hit
TCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAG	6	0.15	No Hit
GGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTT	6	0.15	No Hit
GGCAAGGCCAAGCTGCGCAGCATGAAGGAGCGAAAGCTGGAGAAGAAGCA	6	0.15	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	6	0.15	No Hit
GTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGC	6	0.15	No Hit
CGCAGCTTGGCCTTGCCGATGCCCCCAGCTTGGCGGATGGACTCTAGCAG	6	0.15	No Hit
TAAAAGCATGTTAATCGGCATAAAAAGGCAATGAGATCTTAGGGCACACA	6	0.15	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	6	0.15	No Hit
GAGGGATCTGGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAAC	6	0.15	No Hit
CTTCCTCACTGGAAGGAGATGGTGCTCTTCTTTTTTCTTTCTGAATTGTG	6	0.15	No Hit
CTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCT	6	0.15	No Hit
TCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTG	6	0.15	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACC	6	0.15	No Hit
CTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTC	6	0.15	No Hit
CTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCT	6	0.15	No Hit
AGCAGGAGCAAGTGAGAGCCACGAGCCAAGGTGGGCACTTGATGTCGGAT	5	0.125	No Hit
CTCTGCTCTACCTGCTTCCATCCTCCCTGGTGCGGGGTGGGCCCAGTGAT	5	0.125	No Hit
GGACGACTGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCC	5	0.125	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTT	5	0.125	No Hit
CTTCTGGATTGTGAAATTGGCTCTCTGGGGGCCAAGGCCTTCTAACGTTG	5	0.125	No Hit
CGACACTCAGGTCCAGCCTTGCCAGTCTCAGCCTCCGGTCTCCGTTCCCC	5	0.125	No Hit
CCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAG	5	0.125	No Hit
CTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTGGG	5	0.125	No Hit
TGGGCACTGACTTCTGCAACCACCTGAGCGCGGGCATCCTGTGTGCAGAT	5	0.125	No Hit
CTGGCCCTCCCAGGAGAAGCTGAAGGACTTTCCTGTGTGCGTGAGCACCA	5	0.125	No Hit
CTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCT	5	0.125	No Hit
CGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGC	5	0.125	No Hit
CGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAAC	5	0.125	No Hit
CTCCTTCATGCTGCGCAGCTTGGCCTTGCCGATGCCCCCAGCTTGGCGGA	5	0.125	No Hit
CTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGA	5	0.125	No Hit
GAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAA	5	0.125	No Hit
CACAGAGGCTGGGCTGCTCCCCACCCTCTGCACACCTCCTGCTTCTAACA	5	0.125	No Hit
CTAAAAGCATGTTAATCGGCATAAAAAGGCAATGAGATCTTAGGGCACAC	5	0.125	No Hit
GGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAG	5	0.125	No Hit
GGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTGG	5	0.125	No Hit
CTTCTCCATGGCAGGATCTCCCAGCAGGTAGAGCAGAGCCGGAGCCAGGT	5	0.125	No Hit
CACACGCATCAGAGTTAACTTTGCAGTGAGAGCGGCCTTGCTGCGGCCAA	5	0.125	No Hit
CCCCGCCCCAGCTGTGTGGCCTCAAGCCAGCCTTCCGCTCCTTGAAGCTG	5	0.125	No Hit
TCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTC	5	0.125	No Hit
GGAAGACATACTTCTTCTACCTACAGAGGCGACATGGGGGTCAGGCAAGC	5	0.125	No Hit
ATTGGAGAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGG	5	0.125	No Hit
CGACAGCAGCAGCAGCGCGTCTCCTTCAGGTGGGAGCAGCTCTTTGAGGC	5	0.125	No Hit
CAATTTTTAAAGGCCAGAGGGTAGACTGCAATCACCAAGATGAAATTTAC	5	0.125	No Hit
CAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGT	5	0.125	No Hit
AGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGG	5	0.125	No Hit
CAGGGAGTCACCGGTCGCATGTGACTGAACTCTTCACCCCAGTCTGTGGC	5	0.125	No Hit
GTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGA	5	0.125	No Hit
CCTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGG	5	0.125	No Hit
CCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATG	5	0.125	No Hit
GTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGGGAAGACCCC	5	0.125	No Hit
GAAAAATCAGAGCCAAATGAACCACAGCCCCAAAGGGCACAGTTGAACAA	5	0.125	No Hit
CAGCTCTTTGAGGCCACCTGATTTCTGGCGTGCTCAGTGCACTCGGGTGG	5	0.125	No Hit
CGCGGCCCCTGTAGGCCAAGGCGCCAGGCAGGACGACAGCAGCAGCAGCG	5	0.125	No Hit
AGCTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCT	5	0.125	No Hit
GAAGAAGAAGCAGAAGGAGCAGGAGCAAGTGAGAGCCACGAGCCAAGGTG	5	0.125	No Hit
CTAGCATCCAGAAGTGGCACAAAACCCCTCTGCTGGCTCGTGTGTGCAAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTCT	10	0.0071093175	143.075	6
>>END_MODULE
ERR5296849 read2 length is 75-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5296849_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75-150
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5695	37.0	37.0	37.0	37.0	37.0
2	36.478	37.0	37.0	37.0	37.0	37.0
3	36.622	37.0	37.0	37.0	37.0	37.0
4	36.475	37.0	37.0	37.0	37.0	37.0
5	36.5235	37.0	37.0	37.0	37.0	37.0
6	36.534	37.0	37.0	37.0	37.0	37.0
7	36.509	37.0	37.0	37.0	37.0	37.0
8	36.5895	37.0	37.0	37.0	37.0	37.0
9	36.575	37.0	37.0	37.0	37.0	37.0
10-14	36.5419	37.0	37.0	37.0	37.0	37.0
15-19	36.537	37.0	37.0	37.0	37.0	37.0
20-24	36.5246	37.0	37.0	37.0	37.0	37.0
25-29	36.4704	37.0	37.0	37.0	37.0	37.0
30-34	36.4747	37.0	37.0	37.0	37.0	37.0
35-39	36.44970000000001	37.0	37.0	37.0	37.0	37.0
40-44	36.406400000000005	37.0	37.0	37.0	37.0	37.0
45-49	36.3945	37.0	37.0	37.0	37.0	37.0
50-54	36.3939	37.0	37.0	37.0	37.0	37.0
55-59	36.3574	37.0	37.0	37.0	37.0	37.0
60-64	36.2971	37.0	37.0	37.0	37.0	37.0
65-69	36.28099999999999	37.0	37.0	37.0	37.0	37.0
70-74	36.2546	37.0	37.0	37.0	37.0	37.0
75-79	36.16354640005385	37.0	37.0	37.0	37.0	37.0
80-84	36.18525079996183	37.0	37.0	37.0	37.0	37.0
85-89	36.18538172715895	37.0	37.0	37.0	37.0	37.0
90-94	36.08976990083083	37.0	37.0	37.0	37.0	37.0
95-99	36.04162966006265	37.0	37.0	37.0	37.0	37.0
100-104	35.98570241651052	37.0	37.0	37.0	37.0	37.0
105-109	35.950896683322924	37.0	37.0	37.0	37.0	37.0
110-114	35.93945085523276	37.0	37.0	37.0	37.0	37.0
115-119	35.89153295610675	37.0	37.0	37.0	37.0	37.0
120-124	35.79213665625329	37.0	37.0	37.0	37.0	37.0
125-129	35.79061240235843	37.0	37.0	37.0	37.0	37.0
130-134	35.78695595188631	37.0	37.0	37.0	37.0	37.0
135-139	35.63106227229518	37.0	37.0	37.0	37.0	37.0
140-144	35.62361792418003	37.0	37.0	37.0	37.0	37.0
145-149	35.469198448466834	37.0	37.0	37.0	37.0	37.0
150	35.42593074720125	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	6.0
26	7.0
27	16.0
28	12.0
29	16.0
30	25.0
31	35.0
32	38.0
33	64.0
34	121.0
35	444.0
36	2942.0
37	273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.099999999999998	11.3	10.05	48.55
2	25.4	20.674999999999997	34.849999999999994	19.075
3	25.900000000000002	21.525	19.400000000000002	33.175
4	28.799999999999997	30.8	16.375	24.025
5	31.724999999999998	24.099999999999998	20.175	24.0
6	23.724999999999998	27.725	20.875	27.675
7	23.275000000000002	12.225	34.325	30.175
8	23.425	17.424999999999997	24.15	35.0
9	24.45	17.65	25.624999999999996	32.275
10-14	28.884999999999998	21.265	21.445	28.405
15-19	28.88	21.310000000000002	20.74	29.07
20-24	28.395	21.255	20.565	29.785
25-29	28.67	20.724999999999998	20.79	29.815
30-34	29.465000000000003	20.919999999999998	21.195	28.42
35-39	29.25	21.515	20.645	28.59
40-44	29.235	21.075	20.485	29.205
45-49	29.29	20.365	21.13	29.215000000000003
50-54	29.165000000000003	21.404999999999998	21.15	28.28
55-59	28.715000000000003	20.995	20.195	30.095
60-64	30.145	20.995	20.424999999999997	28.435
65-69	28.705000000000002	20.549999999999997	21.36	29.385
70-74	29.294999999999998	20.325	20.69	29.69
75-79	28.413524057217167	20.846253876162848	19.780934280284086	30.9592877863359
80-84	28.87810248198559	20.031024819855887	21.28702962369896	29.803843074459568
85-89	28.97622027534418	20.500625782227786	19.79474342928661	30.728410513141423
90-94	29.519851799929903	19.731637710909727	20.5627597256296	30.185750763530766
95-99	29.892257579554	20.405913304936107	20.446003507892758	29.255825607617137
100-104	29.719823257682265	20.345450893753767	19.958827073709582	29.975898774854386
105-109	28.912573525715146	21.296063546327485	20.235282288472174	29.556080639485195
110-114	28.691706579279053	20.8469876480968	20.927653138391733	29.533652634232414
115-119	28.981036662452592	20.151706700379265	20.52591656131479	30.341340075853353
120-124	29.35196950444727	19.97966963151207	20.508259212198222	30.160101651842442
125-129	29.692989155338324	20.864518099893083	19.800417494017616	29.64207525075098
130-134	29.74283090111236	21.063373813654454	19.568323298295745	29.62547198693744
135-139	30.774362395443116	19.792682301021195	19.371888951608764	30.061066351926925
140-144	29.76780185758514	20.944272445820435	19.95872033023736	29.32920536635707
145-149	28.948188142456647	20.89606479077977	20.060222199148583	30.09552486761499
150	28.24785212184327	21.244467586566	20.74980473834939	29.75787555324134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	5.0
30	11.0
31	11.0
32	6.5
33	2.5
34	4.5
35	7.5
36	14.5
37	20.5
38	15.5
39	16.5
40	34.0
41	45.0
42	36.0
43	23.0
44	22.5
45	25.5
46	26.5
47	43.0
48	42.0
49	63.5
50	85.5
51	67.0
52	65.0
53	85.0
54	141.5
55	166.5
56	180.5
57	214.0
58	201.5
59	180.5
60	200.0
61	243.5
62	256.5
63	237.0
64	180.0
65	169.0
66	190.0
67	189.0
68	169.5
69	132.5
70	85.0
71	41.0
72	28.5
73	9.5
74	2.5
75	1.5
76	1.0
77	1.5
78	1.0
79	0.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
74-75	1.0
76-77	0.0
78-79	2.0
80-81	0.0
82-83	1.0
84-85	1.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.0
94-95	2.0
96-97	0.0
98-99	2.0
100-101	9.0
102-103	1.0
104-105	0.0
106-107	2.0
108-109	0.0
110-111	12.0
112-113	4.0
114-115	3.0
116-117	5.0
118-119	14.0
120-121	5.0
122-123	1.0
124-125	2.0
126-127	6.0
128-129	1.0
130-131	4.0
132-133	7.0
134-135	11.0
136-137	10.0
138-139	11.0
140-141	6.0
142-143	3.0
144-145	15.0
146-147	7.0
148-149	9.0
150-151	3841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.85
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.51924798567592	33.800000000000004
2	21.665174574753802	24.2
3	9.131602506714414	15.299999999999999
4	3.715308863025962	8.3
5	2.2381378692927485	6.25
6	1.0743061772605194	3.5999999999999996
7	0.7162041181736795	2.8000000000000003
8	0.26857654431512984	1.2
9	0.17905102954341987	0.8999999999999999
>10	0.4923903312444047	3.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	22	0.5499999999999999	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	17	0.42500000000000004	No Hit
CGCAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGG	16	0.4	No Hit
CCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCC	14	0.35000000000000003	No Hit
GTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGAATGGGGAAC	12	0.3	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	12	0.3	No Hit
GTTGGAATGAATGGCCTAGAATCCGACCCAATAAGCCAAAGCCACTTCCA	11	0.27499999999999997	No Hit
GGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTT	11	0.27499999999999997	No Hit
CTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCT	11	0.27499999999999997	No Hit
CAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGAT	10	0.25	No Hit
CTTGTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGG	10	0.25	No Hit
CTAAGATCTCATTGCCTTTTTATGCCGATTAACATGCTTTTAGCCCCTAC	9	0.22499999999999998	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCA	9	0.22499999999999998	No Hit
CGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCT	9	0.22499999999999998	No Hit
AAGAAGGCCATCAAGGTAGTCCCCATACCCCTGTGTCCTGAGGCTACTGG	9	0.22499999999999998	No Hit
ATTTTCTGTGGGTTTGTTAAGTGGTCAGAAATTCTCAATTTTTTGAATAG	8	0.2	No Hit
AGCAGGAGCAAGTGAGAGCCACGAGCCAAGGTGGGGACTTGATGTCGGAT	8	0.2	No Hit
AGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCCAAAGGGCA	8	0.2	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTT	8	0.2	No Hit
CTTAAGTGTTCCATGACAGACTGGTATGAAGGTGGCCACAATTCAGAAAG	8	0.2	No Hit
CTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAG	8	0.2	No Hit
GGACTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCA	7	0.17500000000000002	No Hit
CCACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTC	7	0.17500000000000002	No Hit
CGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACCC	7	0.17500000000000002	No Hit
GGGAAACCCTGGTTCCCCCAGCCCCCGGAGACTTAAATACAGGAAGAAAA	7	0.17500000000000002	No Hit
GTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGGCT	7	0.17500000000000002	No Hit
AAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTCAGAGCAA	7	0.17500000000000002	No Hit
CCCCCACCCTGCAGAGCTGGACCCCTGAGCTAGCCATGCTCTGACAGTCT	7	0.17500000000000002	No Hit
CCACGAGCCAAGGTGGGCACTTGATGTCGGATCTCTTCAACAAGCTGGTC	7	0.17500000000000002	No Hit
CTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAACGTTAGAAGGC	7	0.17500000000000002	No Hit
CAGAGCCGGAGCCAGGTGCAGGCCATTGGAGAGAAGGTCTCCTTGGCCCA	7	0.17500000000000002	No Hit
CCCAGTCGCCCCTGTAGCTCCCCTACCTCCAAGAGCCCAGCCCTTGCCCA	7	0.17500000000000002	No Hit
GTTACACACGCATCAGAGTTAACTTTGCAGTGAGAGCGGCCTTGCTGCGG	7	0.17500000000000002	No Hit
CTTTTAGCCCCTACTGAGCTTATAGTTAACAGAAGTTTCCAGGTCTTTCT	7	0.17500000000000002	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGC	7	0.17500000000000002	No Hit
CCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAGCA	7	0.17500000000000002	No Hit
CTAGCATCCAGAAGTGGCACAAAACCCCTCTGCTGGCTCGTGTGTGCAAC	7	0.17500000000000002	No Hit
GCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGG	6	0.15	No Hit
ATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCA	6	0.15	No Hit
GTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCT	6	0.15	No Hit
AAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCA	6	0.15	No Hit
ATCAGAGTTAACTTTGCAGTGAGAGCGGCCTTGCTGCGGCCAAAGAACAT	6	0.15	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	6	0.15	No Hit
GGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAG	6	0.15	No Hit
CTTAAGAACACAGTGGCGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCA	6	0.15	No Hit
AAAATAGTGGTTTTCAAACTGTAGAGCTCTGGACTTCTCACTTCTAGGGC	6	0.15	No Hit
GTCAGTTTGAGCTGGGTGAGCTTAGAGAGGAAAGCTCCACTATGGCTCCC	6	0.15	No Hit
CTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCGGCAGAGGAGGGATGG	6	0.15	No Hit
CTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGG	6	0.15	No Hit
CTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAG	6	0.15	No Hit
CGCACAGTGCTGGTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAGCAG	6	0.15	No Hit
CTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTGGGCGCCCG	6	0.15	No Hit
AAGAAGGCCATCAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCG	6	0.15	No Hit
CTCCCCTACCTCCAAGAGCCCAGCCCTTGCCCACAGGGCCACACTCCACG	6	0.15	No Hit
CTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGG	6	0.15	No Hit
CGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCG	6	0.15	No Hit
CCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTG	6	0.15	No Hit
CGCAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAA	6	0.15	No Hit
GTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGA	6	0.15	No Hit
GTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGC	6	0.15	No Hit
GTCTTTCTTCACCTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGT	6	0.15	No Hit
GGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTG	5	0.125	No Hit
TGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCT	5	0.125	No Hit
TGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGG	5	0.125	No Hit
GTCAATTAAGACAGCTTGTGCTGGAGGACCCTTTACAGCAGATGAAGGCC	5	0.125	No Hit
AGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGC	5	0.125	No Hit
GGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAG	5	0.125	No Hit
GGCCATCAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGC	5	0.125	No Hit
GTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGT	5	0.125	No Hit
AGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCCAGTGCCAAG	5	0.125	No Hit
ATCTCATTGCCTTTTTATGCCGATTAACATGCTTTTAGCCCCTACTGAGC	5	0.125	No Hit
CCAGGAGCCAGGGGGTGACGGGTGGCTCGGCTCGGGAGGCCTGGGACCCC	5	0.125	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	5	0.125	No Hit
CAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAG	5	0.125	No Hit
CACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCA	5	0.125	No Hit
CAGAGACGCCCCCGCCACAGGATCCAGAGCAAGCACCGCCCCCTGGACGA	5	0.125	No Hit
CCCAGGTCGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGG	5	0.125	No Hit
AATTAAACCAATGGAAAGAAGGGGTCTAATAACAAACTACAGCAACACAT	5	0.125	No Hit
CCCGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGT	5	0.125	No Hit
CTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAG	5	0.125	No Hit
GAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAA	5	0.125	No Hit
ATCTTTTCTGGCTGGGGAGAGGCCTTCATCTGCTGTAAAGGGTCCTCCAG	5	0.125	No Hit
GGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAG	5	0.125	No Hit
CAACATTTAGGTTTTAAAAATCAAGCGTATAAATACAGAAGGTGGAGGGA	5	0.125	No Hit
CTGGAGAAGAAGAAGCAGAAGGAGCAGGAGCAAGTGAGAGCCACGAGCCA	5	0.125	No Hit
GAACAAGTGAGGCTCTGGGTTCCCCATTCCTAATTAAACCAATGGAAAGA	5	0.125	No Hit
GTTTGAGAAGGCAGAGGCGCGACTGGGGTTCATGAGGAAAGGGAGGGGGA	5	0.125	No Hit
CGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCAC	5	0.125	No Hit
CTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGA	5	0.125	No Hit
CTTTGAGGCCACCTGATTTCTGGCGTGCTCAGTGCACTCGGGTGGATTTT	5	0.125	No Hit
CCTGCTTCCATCCTCCCTGGTGCGGGGTGGGCCCAGTGATATCAGCTGCC	5	0.125	No Hit
CTGTTTTCTGTGGTGCTGGATGCACCTCTGTTTTCTGTGGTGGCTCCAAG	5	0.125	No Hit
GTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAGCAGCTTGTCCTGGCT	5	0.125	No Hit
TGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAA	5	0.125	No Hit
GAATGAATGGCCTAGAATCCGACCCAATAAGCCAAAGCCACTTCCACCAA	5	0.125	No Hit
CCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATC	5	0.125	No Hit
GTCGGATTCTAGGCCATTCATTCCAACCTTTAGAGACATCCCAGCTTTCC	5	0.125	No Hit
CTGGCCTTTAAAAATTGTCTGAGCCTTGATTCGATCATGAAACCAGCTTA	5	0.125	No Hit
CCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTATGTGCTGTAC	5	0.125	No Hit
GAGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCCAAAGGGC	5	0.125	No Hit
CCAGAAGTGGCACAAAACCCCTCTGCTGGCTCGTGTGTGCAACTGAGACT	5	0.125	No Hit
CCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTG	5	0.125	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTC	5	0.125	No Hit
GTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGC	5	0.125	No Hit
GAGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCCAGTGCCAA	5	0.125	No Hit
CTTCTTTCAATCAGCAGGGACCGTGCACTCTCTTGGAGCCACCACAGAAA	5	0.125	No Hit
CAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACT	5	0.125	No Hit
CTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCT	5	0.125	No Hit
CTCGAATGCAGAGCTTATGCACCTACCGAAGGTCGTGTCAGCACCCAAGG	5	0.125	No Hit
GTAGACTGCAATCACCAAGATGAAATTTACAAGGAACAAATGTGAAGCCC	5	0.125	No Hit
CTCACGCACACAGGAAAGTCCTTCAGCTTCTCCTGCAGGGCCCGCTCGTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCTCA	10	0.0071093175	143.075	9
CAATCTC	10	0.0071093175	143.075	8
CATCAAT	10	0.0071093175	143.075	5
ACATCAA	10	0.0071093175	143.075	4
GAACATC	10	0.0071093175	143.075	2
ATCAATC	10	0.0071093175	143.075	6
GGAACAT	10	0.0071093175	143.075	1
CTGGCAC	25	9.188525E-4	85.845	1
ACATAGA	25	9.188525E-4	85.845	6
ATAGAAG	25	9.188525E-4	85.845	8
CATAGAA	25	9.188525E-4	85.845	7
TAGAAGT	30	0.001895247	71.5375	9
CACATAG	30	0.001895247	71.5375	5
GCACATA	30	0.001895247	71.5375	4
TGGCACA	35	0.0034925947	61.317856	2
GGCACAT	40	0.005926683	53.653126	3
>>END_MODULE
2025-05-07T12:40:50 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open GL000194.1 as a RefSeq or as a WGS
2025-05-07T12:40:50 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra
Read 4041277 spots for ERR5296849.sra
Written 4041277 spots for ERR5296849.sra

=============================================================
An error occurred during processing.
A report was generated into the file '/home/mark.ziemann@domain.internal.burnet.edu.au/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
SRR ids: ['ERR5296849.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_g1ns6j9l
ERR5296849.sra spots: 64660437
blocks: [[1, 4041277], [4041278, 8082554], [8082555, 12123831], [12123832, 16165108], [16165109, 20206385], [20206386, 24247662], [24247663, 28288939], [28288940, 32330216], [32330217, 36371493], [36371494, 40412770], [40412771, 44454047], [44454048, 48495324], [48495325, 52536601], [52536602, 56577878], [56577879, 60619155], [60619156, 64660437]]
fastq-dump error! exit code: 3
ERR5296849 file size 20271152
ERR5296849 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 16 -o ERR5296849 ERR5296849_1.fastq ERR5296849_2.fastq
Input file:	ERR5296849_1.fastq
Paired file:	ERR5296849_2.fastq
trimmed:	ERR5296849-trimmed-pair1.fastq, ERR5296849-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Wed May  7 12:43:34 2025 >> started

Wed May  7 12:45:31 2025 >> done (116.230s)
60619155 read pairs processed; of these:
      37 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
60619117 (100.00%) read pairs available; of these:
   13570 ( 0.02%) trimmed read pairs available after processing
60605547 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	       2	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       1	  0.00%
 25	       0	  0.00%
 26	       1	  0.00%
 27	       0	  0.00%
 28	       1	  0.00%
 29	       3	  0.00%
 30	       1	  0.00%
 31	       4	  0.00%
 32	      10	  0.00%
 33	      14	  0.00%
 34	      13	  0.00%
 35	      16	  0.00%
 36	      47	  0.00%
 37	     105	  0.00%
 38	     138	  0.00%
 39	     123	  0.00%
 40	     118	  0.00%
 41	     130	  0.00%
 42	     141	  0.00%
 43	     144	  0.00%
 44	     176	  0.00%
 45	     164	  0.00%
 46	     191	  0.00%
 47	     204	  0.00%
 48	     203	  0.00%
 49	     205	  0.00%
 50	     216	  0.00%
 51	     971	  0.00%
 52	     967	  0.00%
 53	     958	  0.00%
 54	     992	  0.00%
 55	    1057	  0.00%
 56	     987	  0.00%
 57	    1123	  0.00%
 58	    1129	  0.00%
 59	    1117	  0.00%
 60	    1154	  0.00%
 61	    1359	  0.00%
 62	    1354	  0.00%
 63	    1315	  0.00%
 64	    1340	  0.00%
 65	    1606	  0.00%
 66	    1645	  0.00%
 67	    1783	  0.00%
 68	    1974	  0.00%
 69	    2071	  0.00%
 70	    2337	  0.00%
 71	    2526	  0.00%
 72	    2772	  0.00%
 73	    3041	  0.01%
 74	    3269	  0.01%
 75	    3751	  0.01%
 76	    4064	  0.01%
 77	    4262	  0.01%
 78	    4837	  0.01%
 79	    5413	  0.01%
 80	    5853	  0.01%
 81	    6286	  0.01%
 82	    7116	  0.01%
 83	    7501	  0.01%
 84	    8178	  0.01%
 85	    8786	  0.01%
 86	    9351	  0.02%
 87	    9716	  0.02%
 88	   10539	  0.02%
 89	   11195	  0.02%
 90	   12127	  0.02%
 91	   12978	  0.02%
 92	   13794	  0.02%
 93	   14434	  0.02%
 94	   15195	  0.03%
 95	   16092	  0.03%
 96	   16856	  0.03%
 97	   17577	  0.03%
 98	   18267	  0.03%
 99	   18969	  0.03%
100	   20234	  0.03%
101	   20816	  0.03%
102	   21708	  0.04%
103	   22198	  0.04%
104	   23272	  0.04%
105	   24089	  0.04%
106	   25403	  0.04%
107	   26152	  0.04%
108	   26934	  0.04%
109	   27759	  0.05%
110	   28590	  0.05%
111	   30176	  0.05%
112	   30674	  0.05%
113	   31881	  0.05%
114	   32819	  0.05%
115	   34540	  0.06%
116	   34735	  0.06%
117	   35876	  0.06%
118	   37419	  0.06%
119	   38408	  0.06%
120	   39731	  0.07%
121	   41229	  0.07%
122	   42006	  0.07%
123	   44114	  0.07%
124	   44839	  0.07%
125	   46379	  0.08%
126	   47161	  0.08%
127	   48706	  0.08%
128	   49818	  0.08%
129	   51784	  0.09%
130	   52949	  0.09%
131	   54850	  0.09%
132	   56078	  0.09%
133	   57912	  0.10%
134	   60074	  0.10%
135	   61779	  0.10%
136	   63469	  0.10%
137	   64679	  0.11%
138	   66306	  0.11%
139	   69619	  0.11%
140	   71946	  0.12%
141	   72773	  0.12%
142	   75186	  0.12%
143	   78272	  0.13%
144	   85810	  0.14%
145	   91038	  0.15%
146	   82876	  0.14%
147	   85553	  0.14%
148	   87315	  0.14%
149	   90609	  0.15%
150	57856219	 95.44%
60619117 reads passed initial QC


criterion=sequence-density
sequence-density=0.45
sequence-density-rank=1
fanout-score=4.01
fanout-score-rank=33
prefix-density=0.53
prefix-fanout=3.4
sequence=GCCTGTAATCCCAGCACTTTGGGAGGCC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=194.91
fanout-score-rank=1
prefix-density=0.13
prefix-fanout=8.7
sequence=AGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT


criterion=sequence-density
sequence-density=0.48
sequence-density-rank=1
fanout-score=4.14
fanout-score-rank=32
prefix-density=0.58
prefix-fanout=3.4
sequence=GCCTGTAATCCCAGCACTTTGGGAGGCC


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=28
fanout-score=223.60
fanout-score-rank=1
prefix-density=0.48
prefix-fanout=19.1
sequence=TTTTTAAAAAACACGTTTGTTATTACCAAAAAGAGACGTCTTTAGGTAAAAATAATAAAAACCCCATGCTGCATAGATAATGCAGATAGTTCTATTTATCTGGTCAACGGGCAAAAAGCAAGCACTTTAGGTCTTCAGCTCCAATCTTTTGTTCATTTCTTATTGCTGGAATTTCATATTTCTTCTTGTTGGATGACTAAACCGGATGATGGTAGAGATGGTAAGCCGGCATTTACTCAGCCCCGCCCTGCTCAGCCTCGGGAGCGGACGAATTCTCAGCTGGTGGATCGGCTGCTTTTGTCTCTTTGCCATCTTGTGGTTTAGGGTTTTCTGGGCGTCTGCGTCGGTAATTGAAGTTGCGGCGGTACCGACGTTGAGGTGGCTGCTGACCTTGGGTCTCATCTCCTTGATTTTCTTTATCTTCTTCATTGCCGTCCTCTCTAGGCTGTCTTTGGCGAGGAGGGCCCCTGCGGAATCGTGGTCTATATCCCCGATACATATTCTGCCTCAC
ERR5296849 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 07 12:46:01
                             Started mapping on |	May 07 12:46:01
                                    Finished on |	May 07 12:50:29
       Mapping speed, Million of reads per hour |	814.29

                          Number of input reads |	60619117
                      Average input read length |	297
                                    UNIQUE READS:
                   Uniquely mapped reads number |	59246363
                        Uniquely mapped reads % |	97.74%
                          Average mapped length |	296.75
                       Number of splices: Total |	56906424
            Number of splices: Annotated (sjdb) |	56262620
                       Number of splices: GT/AG |	56346586
                       Number of splices: GC/AG |	411310
                       Number of splices: AT/AC |	55956
               Number of splices: Non-canonical |	92572
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.85
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.61
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1358583
             % of reads mapped to multiple loci |	2.24%
        Number of reads mapped to too many loci |	1903
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.02%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	14351	14351	14351
N_multimapping	1358583	1358583	1358583
N_noFeature	5930421	31008316	31087496
N_ambiguous	5084979	1100261	1102700
UnstrandedReadsAssigned:48230963 PositiveStrandReadsAssigned:27137786 NegativeStrandReadsAssigned:27056167
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
ERR5296849 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in paired-end mode
[quant] will process pair 1: ERR5296849-trimmed-pair1.fastq
                             ERR5296849-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 60,619,117 reads, 51,886,342 reads pseudoaligned
[quant] estimated average fragment length: 241.443
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,287 rounds

 180870 ERR5296849.ke.tsv
  58302 ERR5296849.se.tsv
 239172 total
==> ERR5296849.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	5	0	0
ENST00000448914.1	13	7.33333	0	0
ENST00000415118.1	8	4	0	0
ENST00000631435.1	12	6.33333	0	0
ENST00000632684.1	12	6.33333	0	0
ENST00000454908.1	17	8.33333	0	0
ENST00000390567.1	20	10.3	0	0
ENST00000603326.1	19	10.3333	0	0
ENST00000414852.1	16	8.25	0	0

==> ERR5296849.se.tsv <==
ENSG00000223972	0
ENSG00000227232	482
ENSG00000278267	2
ENSG00000243485	5
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
ERR5296849 completed mapping pipeline successfully
