Starting /dee2/code/volunteer_pipeline.sh ERR5296899
    current disk space = 1400857696
    free memory = 120872100 
ERR5296899 SRAfilesize
ec1d589e2147b455ce3a380dfec25efc  ERR5296899.sra
ERR5296899.sra file validated
ERR5296899 is paired end
ERR5296899 is conventional basespace
ERR5296899 read1 length is 74-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5296899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	74-150
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4855	37.0	37.0	37.0	37.0	37.0
2	36.5725	37.0	37.0	37.0	37.0	37.0
3	36.609	37.0	37.0	37.0	37.0	37.0
4	36.6575	37.0	37.0	37.0	37.0	37.0
5	36.688	37.0	37.0	37.0	37.0	37.0
6	36.627	37.0	37.0	37.0	37.0	37.0
7	36.7645	37.0	37.0	37.0	37.0	37.0
8	36.79	37.0	37.0	37.0	37.0	37.0
9	36.737	37.0	37.0	37.0	37.0	37.0
10-14	36.7445	37.0	37.0	37.0	37.0	37.0
15-19	36.741200000000006	37.0	37.0	37.0	37.0	37.0
20-24	36.7179	37.0	37.0	37.0	37.0	37.0
25-29	36.72860000000001	37.0	37.0	37.0	37.0	37.0
30-34	36.6765	37.0	37.0	37.0	37.0	37.0
35-39	36.6577	37.0	37.0	37.0	37.0	37.0
40-44	36.6725	37.0	37.0	37.0	37.0	37.0
45-49	36.683299999999996	37.0	37.0	37.0	37.0	37.0
50-54	36.6163	37.0	37.0	37.0	37.0	37.0
55-59	36.614799999999995	37.0	37.0	37.0	37.0	37.0
60-64	36.614399999999996	37.0	37.0	37.0	37.0	37.0
65-69	36.5462	37.0	37.0	37.0	37.0	37.0
70-74	36.5769	37.0	37.0	37.0	37.0	37.0
75-79	36.57709427356839	37.0	37.0	37.0	37.0	37.0
80-84	36.54973242376944	37.0	37.0	37.0	37.0	37.0
85-89	36.524935250723935	37.0	37.0	37.0	37.0	37.0
90-94	36.47881045014415	37.0	37.0	37.0	37.0	37.0
95-99	36.481273502130854	37.0	37.0	37.0	37.0	37.0
100-104	36.37561287740862	37.0	37.0	37.0	37.0	37.0
105-109	36.34944032875395	37.0	37.0	37.0	37.0	37.0
110-114	36.39464293307062	37.0	37.0	37.0	37.0	37.0
115-119	36.39076461330213	37.0	37.0	37.0	37.0	37.0
120-124	36.34751356564721	37.0	37.0	37.0	37.0	37.0
125-129	36.29384678631474	37.0	37.0	37.0	37.0	37.0
130-134	36.31136424653256	37.0	37.0	37.0	37.0	37.0
135-139	36.213805740560005	37.0	37.0	37.0	37.0	37.0
140-144	36.15955956332241	37.0	37.0	37.0	37.0	37.0
145-149	36.141303444137385	37.0	37.0	37.0	37.0	37.0
150	36.1524813576755	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	0.0
24	1.0
25	2.0
26	2.0
27	3.0
28	8.0
29	8.0
30	14.0
31	16.0
32	23.0
33	37.0
34	73.0
35	155.0
36	3082.0
37	575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.425	9.9	10.05	50.625
2	27.725	19.950000000000003	35.75	16.575
3	29.099999999999998	18.8	20.775	31.324999999999996
4	28.925	28.449999999999996	17.4	25.224999999999998
5	32.475	25.8	18.05	23.674999999999997
6	26.575	25.624999999999996	19.6	28.199999999999996
7	23.425	11.525	34.55	30.5
8	25.4	16.575	22.575	35.449999999999996
9	23.5	16.650000000000002	27.525	32.324999999999996
10-14	30.175	20.41	21.075	28.34
15-19	30.06	20.14	20.915	28.884999999999998
20-24	30.385	20.355	21.105	28.155
25-29	29.95	19.695	19.994999999999997	30.36
30-34	30.930000000000003	19.3	21.09	28.68
35-39	30.4	19.900000000000002	20.845	28.854999999999997
40-44	29.845	20.015	19.96	30.18
45-49	31.169999999999998	19.955000000000002	20.255000000000003	28.62
50-54	29.555	20.01	21.495	28.939999999999998
55-59	30.485	21.505	19.535	28.475
60-64	30.080000000000002	20.4	20.745	28.775000000000002
65-69	29.475	19.515	21.285	29.725
70-74	29.054999999999996	20.385	20.655	29.904999999999998
75-79	29.50237559389847	19.83495873968492	19.73493373343336	30.927731932983242
80-84	28.59715829497699	19.541725035021013	21.35781468881329	30.503301981188713
85-89	30.462647706789504	18.921490086120567	20.063088323653115	30.552773883436814
90-94	30.136231593709304	18.806971852148653	21.586697385555446	29.4700991685866
95-99	31.01027826522938	19.18776635748308	21.549260466282277	28.252694911005268
100-104	30.862648667636876	18.96421940081297	20.238871882370653	29.934260049179507
105-109	29.312770019089722	20.410931377474128	20.476238320104493	29.800060283331657
110-114	28.834355828220858	18.998290254450367	22.04063160012069	30.12672231720809
115-119	30.401288958259908	18.5740899249786	21.403756104929258	29.62086501183223
120-124	29.550612800726284	19.680234024310284	20.593130579512785	30.176022595450647
125-129	29.440598312193643	20.435595532871798	19.788771539744303	30.33503461519026
130-134	30.182757049562092	19.921024654482864	19.465397661114768	30.430820634840277
135-139	30.565846231920833	20.137021060644507	19.842679522963717	29.454453184470946
140-144	30.102378648194367	20.572505475474966	20.10900015280395	29.216115723526713
145-149	29.44370453846942	19.62401393299867	19.91599221391251	31.016289314619407
150	28.104911288248907	19.876574955001285	22.242221650809977	29.776292105939827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	5.0
30	10.0
31	12.5
32	9.5
33	4.5
34	5.5
35	3.5
36	7.0
37	12.5
38	11.0
39	15.0
40	27.0
41	31.5
42	22.0
43	16.0
44	21.0
45	21.5
46	24.0
47	38.0
48	42.0
49	42.0
50	42.5
51	54.5
52	61.0
53	73.0
54	103.5
55	145.0
56	179.5
57	198.5
58	204.5
59	208.5
60	258.0
61	266.5
62	251.0
63	234.5
64	180.0
65	200.5
66	214.0
67	189.5
68	183.5
69	159.0
70	108.0
71	55.5
72	32.5
73	12.0
74	4.0
75	2.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
74-75	1.0
76-77	0.0
78-79	1.0
80-81	0.0
82-83	2.0
84-85	2.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.0
94-95	3.0
96-97	0.0
98-99	2.0
100-101	1.0
102-103	3.0
104-105	2.0
106-107	0.0
108-109	0.0
110-111	5.0
112-113	2.0
114-115	0.0
116-117	4.0
118-119	1.0
120-121	4.0
122-123	3.0
124-125	3.0
126-127	2.0
128-129	3.0
130-131	5.0
132-133	1.0
134-135	5.0
136-137	5.0
138-139	7.0
140-141	4.0
142-143	5.0
144-145	10.0
146-147	15.0
148-149	8.0
150-151	3889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.79765395894429	30.075000000000003
2	19.990224828934505	20.45
3	9.872922776148583	15.15
4	4.838709677419355	9.9
5	1.906158357771261	4.875
6	1.7595307917888565	5.4
7	0.7331378299120235	2.625
8	0.43988269794721413	1.7999999999999998
9	0.635386119257087	2.9250000000000003
>10	1.0263929618768328	6.800000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGG	23	0.575	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	20	0.5	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	18	0.44999999999999996	No Hit
CGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCT	18	0.44999999999999996	No Hit
CGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACCC	14	0.35000000000000003	No Hit
GTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	14	0.35000000000000003	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	13	0.325	No Hit
GTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGC	13	0.325	No Hit
CTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAG	13	0.325	No Hit
CTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCGGCA	12	0.3	No Hit
CTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCA	11	0.27499999999999997	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	11	0.27499999999999997	No Hit
GGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTC	11	0.27499999999999997	No Hit
CTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCT	11	0.27499999999999997	No Hit
CTTCATACCAGTCTGTCATGGAACACTTAAGCCGCTTGAGTGCCTGCTGG	10	0.25	No Hit
AATTAAACCAATGGAAAGAAGGGGTCTAATAACAAACTACAGCAACACAT	10	0.25	No Hit
CCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCC	10	0.25	No Hit
GTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGGGAAGACCCC	10	0.25	No Hit
GTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGC	10	0.25	No Hit
GGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGCA	10	0.25	No Hit
CGCCTCTGTAGGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGC	10	0.25	No Hit
GGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAACATCAGCTCTG	9	0.22499999999999998	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	9	0.22499999999999998	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTT	9	0.22499999999999998	No Hit
CTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGG	9	0.22499999999999998	No Hit
GGACTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAG	9	0.22499999999999998	No Hit
GGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTT	9	0.22499999999999998	No Hit
GTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGAATGGGGAAC	9	0.22499999999999998	No Hit
GGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGG	9	0.22499999999999998	No Hit
CTTGTCCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGG	9	0.22499999999999998	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTC	9	0.22499999999999998	No Hit
CTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCT	9	0.22499999999999998	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACC	9	0.22499999999999998	No Hit
CTAGCATCCAGAAGTGGCACAAAACCCCTCTGCTGGCTCGTGTGTGCAAC	9	0.22499999999999998	No Hit
CACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCA	8	0.2	No Hit
CTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAG	8	0.2	No Hit
CTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTGGGCGCCCG	8	0.2	No Hit
GTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCA	8	0.2	No Hit
CCCAGCTGTGTGGCCTCAGGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCC	8	0.2	No Hit
GCTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTGG	8	0.2	No Hit
CGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAG	8	0.2	No Hit
CTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAG	8	0.2	No Hit
TGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTG	8	0.2	No Hit
TGGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGT	7	0.17500000000000002	No Hit
CTTCCATCCTCCCTGGTGCGGGGTGGGCCCAGTGATATCAGCTGCCTGCT	7	0.17500000000000002	No Hit
GTTCTGGAATGGTGCCAGGGGCAGAGGGGGCAATGCCGGGGCCCAGGTCG	7	0.17500000000000002	No Hit
CGTCACCCCCTCCCAAGGAAGTAGGTCTGAGCAGCTTGTCCTGGCTGTGT	7	0.17500000000000002	No Hit
ATCAGACACAGGTACAGCACATAGGCCAGGAGCCAGGGGGTGACGGGTGG	7	0.17500000000000002	No Hit
CGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAAC	7	0.17500000000000002	No Hit
CCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGC	7	0.17500000000000002	No Hit
CTTTGAGGCCACCTGATTTCTGGCGTGCTCAGTGCACTCGGGTGGATTTT	7	0.17500000000000002	No Hit
GAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCG	7	0.17500000000000002	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	7	0.17500000000000002	No Hit
CAGGGGGTGACGGGTGGCTCGGCTCGGGAGGCCTGGGACCCCACAGTGCA	7	0.17500000000000002	No Hit
CTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGA	7	0.17500000000000002	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	7	0.17500000000000002	No Hit
TGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCTCAGGC	7	0.17500000000000002	No Hit
CTCAGACCTACTTCCTTGGGAGGGGGTGACGGAACCAGCACTGTGTGGAG	7	0.17500000000000002	No Hit
ATTTTCTGTGGGTTTGTTAAGTGGTCAGAAATTCTCAATTTTTTGAATAG	6	0.15	No Hit
GTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGC	6	0.15	No Hit
ATCAGAGTTAACTTTGCAGTGAGAGCGGCCTTGCTGCGGCCAAAGAACAT	6	0.15	No Hit
CTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCC	6	0.15	No Hit
GTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGC	6	0.15	No Hit
CACGAGCCAAGGTGGGCACTTGATGTCGGATCTCTTCAACAAGCTGGTCA	6	0.15	No Hit
CTGATTTCTGGCGTGCTCAGTGCACTCGGGTGGATTTTCTGTGGGTTTGT	6	0.15	No Hit
GGAAGACATACTTCTTCTACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAG	6	0.15	No Hit
TGGGAACTAGGGTCAATTAAGACAGCTTGTGCTGGAGGACCCTTTACAGC	6	0.15	No Hit
TGGCCATACAGGTTCTCGGTGGTGTTGAAGAGCAGCAAGGAGCTGACAGA	6	0.15	No Hit
CGACACTCAGGTCCAGCCTTGCCAGTCTCAGCCTCCGGTCTCCGTTCCCC	6	0.15	No Hit
CTTGCTCTGGATCCTGTGGCGGGGGCGTCTCTGCAGGCCAGGGTCCTGGG	6	0.15	No Hit
CTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCT	6	0.15	No Hit
CTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGGACCTG	6	0.15	No Hit
GAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGGGAAGACCCCCAA	6	0.15	No Hit
CTTTCCTGTGTGCGTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAG	6	0.15	No Hit
GAAGAAGCAGAAGGAGCAGGAGCAAGTGAGAGCCACGAGCCAAGGTGGGG	6	0.15	No Hit
GAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAA	6	0.15	No Hit
CAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGGACCTGGGG	6	0.15	No Hit
CTTTAGGGCACGGGGACTTCTGGATTGTGAAATTGGCTCTCTGGGGGCCA	6	0.15	No Hit
CACAGGTACAGCACATAGGCCAGGAGCCAGGGGGTGACGGGTGGCTCGGC	6	0.15	No Hit
CCGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACC	6	0.15	No Hit
GTCGCATGTGACTGAACTCTTCACCCCAGTCTGTGGCTTTCCCGTTGCAG	6	0.15	No Hit
CACACGCATCAGAGTTAACTTTGCAGTGAGAGCGGCCTTGCTGCGGCCAA	6	0.15	No Hit
GAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCCAAAGGGCAC	6	0.15	No Hit
ATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCACCGAGAACCTGTA	6	0.15	No Hit
TGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAA	6	0.15	No Hit
CTGGGATGTCTCTAAAGGTTGGAATGAATGGCCTAGAATCCGACCCAATA	6	0.15	No Hit
CTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTTCTCTGGGACCT	6	0.15	No Hit
CGGGCATCCTGTGTGCAGATACTCCCTGCTTCCTCTCTAGCCCCCACCCT	6	0.15	No Hit
GTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAAAGTCCTTCAGCT	6	0.15	No Hit
GTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGA	6	0.15	No Hit
CGAGGACGACTGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGA	6	0.15	No Hit
CTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCC	6	0.15	No Hit
GGAGAAGAAGCAGCAGAAGGAGCAGGAGCAAGGTGAGCGGGCCCTGGAGC	6	0.15	No Hit
GAGCAAGTGAGAGCCACGAGCCAAGGTGGGCACTTGATGTCGGATCTCTT	6	0.15	No Hit
CCTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGG	5	0.125	No Hit
GAAGGAGCAGGAGCAAGTGAGAGCCACGAGCCAAGGTGGGGACTTGATGT	5	0.125	No Hit
CCCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACG	5	0.125	No Hit
CGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCC	5	0.125	No Hit
GTGAGCACCAAGCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGG	5	0.125	No Hit
CGTCTCTGCAGGCCAGGGTCCTGGGCGCCCGTGAAGATGGAGCCATATTC	5	0.125	No Hit
ATTTATACGCTTGATTTTTAAAACCTAAATGTTGGGCTTCACATTTGTTC	5	0.125	No Hit
CCACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTC	5	0.125	No Hit
CTTTCTTCACCTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGTAT	5	0.125	No Hit
CTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCAGAGGGGG	5	0.125	No Hit
CGGCTACCTCAGTGTGGAAGGTGGGCAGTTCTGGAATGGTGCCAGGGGCA	5	0.125	No Hit
GGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCC	5	0.125	No Hit
CCCAGGTCGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGG	5	0.125	No Hit
CTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTG	5	0.125	No Hit
CTGAACTCTTCACCCCAGTCTGTGGCTTTCCCGTTGCAGTGAGAGCCACG	5	0.125	No Hit
GGAAAAGGCTGAGTGAGGGTGGTTGGTGGGAAACCCTGGTTCCCCCAGCC	5	0.125	No Hit
CAGGAGCAAGTGAGAGCCACGAGCCAAGGTGGGCACTTGATGTCGGATCT	5	0.125	No Hit
GCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGC	5	0.125	No Hit
GTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCT	5	0.125	No Hit
CTGGCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGA	5	0.125	No Hit
CTGGAGCTTGCAGTCGGAGGGCCTTGGGCAAGATCGCCTCCTCCCCTCCA	5	0.125	No Hit
CTTCTTTCCATTGGTTTAATTAGGAATGGGGAACCCAGAGCCTCACTTGT	5	0.125	No Hit
CCCCACCCCAGCTGTGTGGCCTCAGGCCAGCCTTCCGCTCCTTGAAGCTG	5	0.125	No Hit
GTCATGAGGCGCAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGG	5	0.125	No Hit
ATTTTGACGGCTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGG	5	0.125	No Hit
CTTCCAGCATGTGCCCTAGGGGAAGCAGGGGCCAGCTGGCAAGAGCAGGG	5	0.125	No Hit
CAGACACAGGTACAGCACATAGGCCAGGAGCCAGGGGGTGACGGGTGGCT	5	0.125	No Hit
GATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGG	5	0.125	No Hit
CTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGTATTTATACGCTT	5	0.125	No Hit
CGCAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAA	5	0.125	No Hit
CTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTTCAACACCAC	5	0.125	No Hit
TGGGCTCAGGACAGCGGGTGTCAGCTTGCCTGACCCCCATGTCGCCTCTG	5	0.125	No Hit
TGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCG	5	0.125	No Hit
GTTCCCTCCACCTTCTGTATTTATACGCTTGATTTTTAAAACCTAAATGT	5	0.125	No Hit
GGAAGACCCCCAAGTCCCTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGT	5	0.125	No Hit
GTCTTTCTTCACCTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGT	5	0.125	No Hit
CTTCTAACAAAATCTTGTAGACAAGATGGAGCTATGGGGGTTGGAGGAGA	5	0.125	No Hit
CTCAGACTTGTCCCTGTGTCCACAGGTGTTCTCCAGTGCCAAGTACCCTG	5	0.125	No Hit
AGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGTG	10	0.0070282277	143.625	3
AGCAAGT	10	0.0070282277	143.625	2
>>END_MODULE
ERR5296899 read2 length is 74-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR5296899_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	74-150
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.7295	37.0	37.0	37.0	37.0	37.0
2	36.6115	37.0	37.0	37.0	37.0	37.0
3	36.7365	37.0	37.0	37.0	37.0	37.0
4	36.6705	37.0	37.0	37.0	37.0	37.0
5	36.6885	37.0	37.0	37.0	37.0	37.0
6	36.5875	37.0	37.0	37.0	37.0	37.0
7	36.645	37.0	37.0	37.0	37.0	37.0
8	36.7	37.0	37.0	37.0	37.0	37.0
9	36.7015	37.0	37.0	37.0	37.0	37.0
10-14	36.6588	37.0	37.0	37.0	37.0	37.0
15-19	36.6706	37.0	37.0	37.0	37.0	37.0
20-24	36.6245	37.0	37.0	37.0	37.0	37.0
25-29	36.632	37.0	37.0	37.0	37.0	37.0
30-34	36.5848	37.0	37.0	37.0	37.0	37.0
35-39	36.5567	37.0	37.0	37.0	37.0	37.0
40-44	36.528200000000005	37.0	37.0	37.0	37.0	37.0
45-49	36.5392	37.0	37.0	37.0	37.0	37.0
50-54	36.4802	37.0	37.0	37.0	37.0	37.0
55-59	36.438	37.0	37.0	37.0	37.0	37.0
60-64	36.400999999999996	37.0	37.0	37.0	37.0	37.0
65-69	36.3806	37.0	37.0	37.0	37.0	37.0
70-74	36.37239999999999	37.0	37.0	37.0	37.0	37.0
75-79	36.33593398349588	37.0	37.0	37.0	37.0	37.0
80-84	36.304380067911836	37.0	37.0	37.0	37.0	37.0
85-89	36.300306666205515	37.0	37.0	37.0	37.0	37.0
90-94	36.25102272647449	37.0	37.0	37.0	37.0	37.0
95-99	36.16841313612434	37.0	37.0	37.0	37.0	37.0
100-104	36.14560383501702	37.0	37.0	37.0	37.0	37.0
105-109	36.099964181948636	37.0	37.0	37.0	37.0	37.0
110-114	36.10149005784033	37.0	37.0	37.0	37.0	37.0
115-119	35.984834888777584	37.0	37.0	37.0	37.0	37.0
120-124	35.83334979669601	37.0	37.0	37.0	37.0	37.0
125-129	35.928604094840594	37.0	37.0	37.0	37.0	37.0
130-134	35.955717491218	37.0	37.0	37.0	37.0	37.0
135-139	35.7595479775324	37.0	37.0	37.0	37.0	37.0
140-144	35.74676524385474	37.0	37.0	37.0	37.0	37.0
145-149	35.59460122705201	37.0	37.0	37.0	37.0	37.0
150	35.69323733607611	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	5.0
25	4.0
26	3.0
27	8.0
28	12.0
29	15.0
30	16.0
31	25.0
32	26.0
33	61.0
34	104.0
35	331.0
36	3022.0
37	366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.7	11.3	10.45	48.55
2	26.200000000000003	21.2	33.1	19.5
3	27.800000000000004	21.95	18.95	31.3
4	28.7	29.65	15.725	25.924999999999997
5	31.35	25.15	20.1	23.400000000000002
6	24.8	27.775	18.275	29.15
7	22.325	12.025	34.825	30.825000000000003
8	23.400000000000002	16.6	22.025	37.974999999999994
9	24.075	16.075	26.625	33.225
10-14	29.14	20.985	21.25	28.625
15-19	29.785	20.59	20.7	28.925
20-24	29.265	20.21	20.990000000000002	29.535
25-29	29.285	20.65	19.475	30.59
30-34	29.685	20.36	21.08	28.875
35-39	29.525000000000002	21.965	19.57	28.939999999999998
40-44	30.03	19.919999999999998	20.445	29.604999999999997
45-49	30.225	20.315	19.845	29.615000000000002
50-54	29.299999999999997	21.39	19.384999999999998	29.925
55-59	30.159999999999997	21.115000000000002	18.755	29.970000000000002
60-64	29.755	21.115000000000002	19.99	29.14
65-69	29.965000000000003	20.745	20.22	29.07
70-74	29.975	19.79	20.595	29.64
75-79	28.46211552888222	20.065016254063515	19.254813703425857	32.21805451362841
80-84	29.107464478687213	20.187112267360416	20.252151290774464	30.453271963177908
85-89	29.125776086521128	19.992990186260766	19.602443420789104	31.278790306429
90-94	30.011018731844135	19.072423119302815	20.38966242612441	30.526895722728636
95-99	30.609175231887694	20.185510152920532	19.659062421659566	29.54625219353221
100-104	30.185175892005823	19.82235158327897	19.074622371656545	30.917850153058662
105-109	30.126594996483476	21.48598412538933	19.230382799156036	29.157038078971166
110-114	29.457910087498746	20.335914713869055	20.240370109624862	29.96580508900734
115-119	29.70142490307638	20.366547505160867	20.059412919792557	29.872614671970194
120-124	29.994452009885507	20.456952640338933	19.614666868411764	29.933928481363797
125-129	30.006569306180204	20.981353277073122	18.626509677093335	30.38556773965334
130-134	30.820634840277428	20.65002784387182	19.11102111071736	29.4183162051334
135-139	30.728241563055064	20.588683075361583	18.665313372240547	30.017761989342805
140-144	29.705088371619215	21.066571588651758	18.718484184790913	30.509855854938117
145-149	29.218317795307858	20.336031144349963	19.823788546255507	30.621862514086672
150	28.64489586011828	21.470815119568012	19.465158138338904	30.419130881974798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	7.0
30	11.0
31	13.0
32	13.5
33	7.5
34	4.0
35	4.0
36	9.0
37	12.5
38	10.5
39	10.5
40	15.5
41	22.5
42	23.5
43	16.5
44	17.5
45	16.5
46	15.5
47	33.5
48	44.0
49	49.5
50	56.0
51	58.5
52	60.5
53	78.5
54	119.5
55	160.0
56	188.0
57	201.5
58	199.0
59	205.0
60	223.0
61	225.0
62	243.5
63	271.0
64	222.5
65	194.0
66	196.5
67	192.0
68	187.5
69	154.5
70	100.5
71	54.0
72	34.5
73	11.0
74	3.5
75	1.0
76	0.5
77	0.5
78	0.5
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
74-75	1.0
76-77	0.0
78-79	1.0
80-81	0.0
82-83	2.0
84-85	2.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	2.0
94-95	3.0
96-97	0.0
98-99	2.0
100-101	1.0
102-103	3.0
104-105	2.0
106-107	0.0
108-109	0.0
110-111	5.0
112-113	2.0
114-115	0.0
116-117	4.0
118-119	1.0
120-121	4.0
122-123	3.0
124-125	3.0
126-127	2.0
128-129	3.0
130-131	5.0
132-133	1.0
134-135	5.0
136-137	5.0
138-139	7.0
140-141	4.0
142-143	5.0
144-145	10.0
146-147	15.0
148-149	8.0
150-151	3889.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.900000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.647495361781075	32.15
2	21.382189239332096	23.05
3	9.461966604823747	15.299999999999999
4	3.710575139146568	8.0
5	2.1335807050092765	5.75
6	1.2987012987012987	4.2
7	0.6957328385899815	2.625
8	0.6029684601113172	2.6
9	0.5102040816326531	2.475
>10	0.5565862708719851	3.85
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACA	22	0.5499999999999999	No Hit
CGCAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGG	15	0.375	No Hit
CAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGAT	13	0.325	No Hit
GTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGA	13	0.325	No Hit
GGAACATCAATCTCAGGCACCTGGCCCAGGTCTGGCACATAGAAGTAGTT	13	0.325	No Hit
CTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGC	12	0.3	No Hit
GGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCCTC	12	0.3	No Hit
GCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTC	11	0.27499999999999997	No Hit
GTCAGTTTGAGCTGGGTGAGCTTAGAGAGGAAAGCTCCACTATGGCTCCC	11	0.27499999999999997	No Hit
CAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGGACCTGGGG	11	0.27499999999999997	No Hit
TGGGAATCGTAGGGGGCTCCATGACACCTTCCCCCCCAGACCCAGACTTG	11	0.27499999999999997	No Hit
CTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGAAGCAGGTCTGAG	10	0.25	No Hit
GTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCATGTGATGCT	9	0.22499999999999998	No Hit
CAGAAATTCTCAATTTTTTGAATAGTTTCCATTTCAAATATCTTGTTCTA	9	0.22499999999999998	No Hit
CCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATCTTCACGGGCGCCC	9	0.22499999999999998	No Hit
CTTGGGGGTCTTCCCAGCAACATCAGCTCTGTCAGCTCCTTGCTGCTCTT	9	0.22499999999999998	No Hit
GTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGGCT	9	0.22499999999999998	No Hit
CACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCA	9	0.22499999999999998	No Hit
CGAAAGCTGGAGAAGAAGAAGCAGAAGGAGCAGGAGCAAGTGAGAGCCAC	9	0.22499999999999998	No Hit
CCCACCCAGGGAAGCAGGTCTGAGCAGCTTGTCCTGGCTGTGTCCATGTC	9	0.22499999999999998	No Hit
CGCTCGTCCAGGGGGCGGTGCTTGCTCTGGATCCTGTGGCGGGGGCGTCT	9	0.22499999999999998	No Hit
GGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGG	9	0.22499999999999998	No Hit
CTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAGACATACTTCTTCTACC	9	0.22499999999999998	No Hit
GCTGTGTCCATGTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGG	8	0.2	No Hit
AAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGCGTGTC	8	0.2	No Hit
GTTGGAATGAATGGCCTAGAATCCGACCCAATAAGCCAAAGCCACTTCCA	8	0.2	No Hit
CGCTCCTTGAAGCTGGTCTCCACACAGTGCTGGTTCCGTCACCCCCTCCC	8	0.2	No Hit
CTCTTCTGCATCGTCCTCGGGCTCCGGCTTGGTGCTCACGCACACAGGAA	8	0.2	No Hit
ATTCATTCCAACCTTTAGAGACATCCCAGCTTTCCCTAGCCCAGAGTCTG	8	0.2	No Hit
GAACACTTAAGCCGCTTGAGTGCCTGCTGGTACTCCCAGCCCTGCCATGC	8	0.2	No Hit
GTCAGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGC	8	0.2	No Hit
GTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAGAAGGC	8	0.2	No Hit
CTCAGTTCTTTATTGATTGGTGTGCCGTTTTCTCTGGAAGCCTCTTAAGA	8	0.2	No Hit
CTGCTCTCTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTG	8	0.2	No Hit
AGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAAG	8	0.2	No Hit
CGCCTCTGTAGGTAGAAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGC	8	0.2	No Hit
GTTTTCTCTGGAAGCCTCTTAAGAACACAGTGGCGCAGGCTGGGTGGAGC	7	0.17500000000000002	No Hit
CCACGAGCCAAGGTGGGGACTTGATGTCGGATCTCTTCAACAAGCTGGTC	7	0.17500000000000002	No Hit
GGGGAAGGTGTCATGGAGCCCCCTACGATTCCCAGTCGTCCTCGTCCTCC	7	0.17500000000000002	No Hit
TGGGCACTGACTTCTGCAACCACCTGAGCGCGGGCATCCTGTGTGCAGAT	7	0.17500000000000002	No Hit
CGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCAAC	7	0.17500000000000002	No Hit
GAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCAAGGGCAGCAA	7	0.17500000000000002	No Hit
CTCAGGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTT	7	0.17500000000000002	No Hit
CAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAG	7	0.17500000000000002	No Hit
GTTTGTTATTAGACCCCTTCTTTCCATTGGTTTAATTAGGAATGGGGAAC	7	0.17500000000000002	No Hit
CAGAGCTGGACCCCTGAGCTAGCCATGCTCTGACAGTCTCAGTTGCACAC	7	0.17500000000000002	No Hit
CAAGATTGAGAAGATCAAGGGCAGCAAGAAGGCCATCAAGGTGTTCTCCA	7	0.17500000000000002	No Hit
GTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAGGATGGAACATCAATCT	7	0.17500000000000002	No Hit
GTTTGATGCCCCCTTGTCCATCAGCAAGAGAGAGCAGCTGGAACAGCAGG	7	0.17500000000000002	No Hit
CAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCCCTAC	7	0.17500000000000002	No Hit
CAAGCACCGCCCCCTGGACGAGCGGGCCCTGCAGGAGAAGCTGAAGGACT	7	0.17500000000000002	No Hit
CAGGAATATGGCTCCATCTTCACGGGCGCCCAGGACCCTGGCCTGCAGAG	6	0.15	No Hit
ATTTTCTGTGGGTTTGTTAAGTGGTCAGAAATTCTCAATTTTTTGAATAG	6	0.15	No Hit
AAGAAGTATGTCTTCCTGGACCCCCTGGCTGGTGCTGTAACAAAGACCCA	6	0.15	No Hit
GCAGCCCAGCCTCTGTGATCTTCTCCATGGCAGGATCTCCCAGCAGGTAG	6	0.15	No Hit
CGGGCGCCCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAG	6	0.15	No Hit
AGAACATATAGGAAAAATCAGAGCCAAATGAACCACAGCCCCAAAGGGCA	6	0.15	No Hit
GGGAAACCCTGGTTCCCCCAGCCCCCGGAGACTTAAATACAGGAAGAAAA	6	0.15	No Hit
CAGAGAACTACTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGAT	6	0.15	No Hit
CAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAG	6	0.15	No Hit
GGCAGAGTCCATCCAGTTCATTCAACACCTGGTTAGAAAACTGGGGCCAG	6	0.15	No Hit
CTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGGACCTG	6	0.15	No Hit
GGCCATTGGAGAGAAGGTCTCCTTGGCCCAGGCCAAGATTGAGAAGATCA	6	0.15	No Hit
AAGAAGGCCATCAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGCG	6	0.15	No Hit
CTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGCCCCCAG	6	0.15	No Hit
CCCGGAGGAGCCTTTGCCCGCGTGTCAGACTCCATCCCTCCTCTGCCGCC	6	0.15	No Hit
CTTCTATGTGCCAGACCTGGGCCAGGTGCCTGAGATTGATGTTCCATCCT	6	0.15	No Hit
CGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGGCAGGTAG	6	0.15	No Hit
GATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGG	6	0.15	No Hit
CCCGAGCCGAGCCACCCGTCACCCCCTGGCTCCTGGCCTATGTGCTGTAC	6	0.15	No Hit
CTGAACTGTGTCTAAAGCAAGTTCCCTCCACCTTCTGTATTTATACGCTT	6	0.15	No Hit
CTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGGAGGACG	6	0.15	No Hit
CTGTAACAAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTG	6	0.15	No Hit
CTGGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTC	6	0.15	No Hit
CAAGAAGGCCATCAAGGTGTTCTCCAGTGCCAAGTACCCTGCTCCAGGGC	6	0.15	No Hit
CTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACCCAGGGA	6	0.15	No Hit
GTTCCATCCTACCTGCCTGACCTGCCCGGCATTGCCAACGACCTCATGTA	6	0.15	No Hit
CTCTTGCTGATGGACAAGGGGGCATCAAACAGCTTCTCCTCTGTCTCTGC	6	0.15	No Hit
AAAGACCCATGTGATGCTGGGGGCAGAGACAGAGGAGAAGCTGTTTGATG	6	0.15	No Hit
GCAGGAGCAAGTGAGAGCCACGAGCCAAGGTGGGCACTTGATGTCGGATC	5	0.125	No Hit
ATCAGAGTTAACTTTGCAGTGAGAGCGGCCTTGCTGCGGCCAAAGAACAT	5	0.125	No Hit
GTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGC	5	0.125	No Hit
CGCAGGCTGGGTGGAGCCGTCCCCCCATGGAGCACAGGCAGACAGAAGTC	5	0.125	No Hit
CTCTGCTCTACCTGCTGGGAGATCCTGCCATGGAGAAGATCACAGAGGCT	5	0.125	No Hit
GGAACCCAGAGCCTCACTTGTTCAGGCTCCCTCTGCCCTAGAAGTGAGAA	5	0.125	No Hit
CTTCTCCTCTGTCTCTGCCCCCAGCATCACATGGGTCTTTGTTACAGCAC	5	0.125	No Hit
CTCAGGCAGGGCTGGGGAAGCTTACTGTGTCCAAGAGCCTGCTGGGAGGG	5	0.125	No Hit
CGGATCTCTTCAACAAGCTGGTCATGAGGCGCAAGGGCATCTCTGGGAAA	5	0.125	No Hit
CAGCTCCTGAGGTGCTGGCCAGTGCACCCCCACTCCCACCCTCAACCGCG	5	0.125	No Hit
CGTCTCTGCAGGCCAGGGTCCTGGGCGCCCGTGAAGATGGAGCCATATTC	5	0.125	No Hit
CCAGCTTCAAGGAGCGGAAGGCTGGCTTGAGGCCACACAGCTGGGGCGGG	5	0.125	No Hit
CGCTCCTTGAAGCTGGTCTCCGCACAGTGCTGGTTCCATCACCCCCACCC	5	0.125	No Hit
TCACCCCCTGGCTCCTGGCCTATGTGCTGTACCTGTGTCTGATGCCCTGG	5	0.125	No Hit
AGAGCAATGGCCCAAGTCTGGGTCTGGGGGGGAAGGTGTCATGGAGCCCC	5	0.125	No Hit
CCAGGACCCTGGCCTGCAGAGACGCCCCCGCCACAGGATCCAGAGCAAGC	5	0.125	No Hit
TTCAAACTGTAGAGCTCTGGACTTCTCACTTCTAGGGCAGAGGGAGCCTG	5	0.125	No Hit
AGCAGGAGCAAGGTGAGCGGGCCCTGGAGCTTGCAGTCGGAGGGCCTTGG	5	0.125	No Hit
CCAGCATCACATGGGTCTTTGTTACAGCACCAGCCAGGGGGTCCAGGAAG	5	0.125	No Hit
CCCAGGTCGGCAATGTACATGAGGTCGTTGGCAATGCCGGGCAGGTCAGG	5	0.125	No Hit
GCCGGAGCCCGAGGACGATGCAGAAGAGGGACTTGGGGGTCTTCCCAGCA	5	0.125	No Hit
CTACGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTG	5	0.125	No Hit
GGGAGATCCTGCCATGGAGAAGATCACAGAGGCTGGGCTGCTCCCCACCC	5	0.125	No Hit
CAACGACCTCATGTACATTGCCGACCTGGGCCCCGGCATTGCCCCCTCTG	5	0.125	No Hit
GAACAAGTGAGGCTCTGGGTTCCCCATTCCTAATTAAACCAATGGAAAGA	5	0.125	No Hit
CTGGTCATGAGGCGCAAGGGCATCTCTGGGAAAGGACCTGGGGCTGGTGA	5	0.125	No Hit
CTTCTGGATGCTAGGGTTACACTGGGAGACACAGCAGTGAAGCTGAAATG	5	0.125	No Hit
CTTGTCCTGGCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGG	5	0.125	No Hit
CGATTCCCAGTCGTCCTCGTCCTCCTCTGCCTGTGGCTGCTGCGGTGGCG	5	0.125	No Hit
CCCAGACTTGGGCCGTTGCTCTGACATGGACACAGCCAGGACAAGCTGCT	5	0.125	No Hit
CTAGAGTCCATCCGCCAAGCTGGGGGCATCGGCAAGGCCAAGCTGCGCAG	5	0.125	No Hit
GGCACATAGAAGTAGTTCTCTGGGACCTGCTGTTCCAGCTGCTCTCTCTT	5	0.125	No Hit
GCCAAGTACCCTGCTCCAGGGCGCCTGCAGGAATATGGCTCCATCTTCAC	5	0.125	No Hit
CGGGCATCCTGTGTGCAGATACTCCCTGCTTCCTCTCTAGCCCCCACCCT	5	0.125	No Hit
TGGGAGTACCAGCAGGCACTCAAGCGGCTTAAGTGTTCCATGACAGACTG	5	0.125	No Hit
CTGGGAAAGGACCTGGGGCTGGTGAGGGGCCCGGAGGAGCCTTTGCCCGC	5	0.125	No Hit
CTCCACACAGTGCTGGTTCCGTCACCCCCTCCCAAGGAAGTAGGTCTGAG	5	0.125	No Hit
GTTGAAGAGCAGCAAGGAGCTGACAGAGCTGATGTTGCTGGGAAGACCCC	5	0.125	No Hit
GTCAGACTCCATCCCTCCTCTGCCGCCACCGCAGCAGCCACAGGCAGAGG	5	0.125	No Hit
CTCGGCTCGGGAGGCCTGGGACCCCACAGTGCACGCTGTGCCCCTGATGA	5	0.125	No Hit
TGTAGCTCCCCTACCTCCAAGAGCCCAGCCCTTGCCCACAGGGCCACACT	5	0.125	No Hit
GCTGTGTGGCCTCAGGCCAGCCTTCCGCTCCTTGAAGCTGGTCTCCGCAC	5	0.125	No Hit
CTCAATCTTGGCCTGGGCCAAGGAGACCTTCTCTCCAATGGCCTGCACCT	5	0.125	No Hit
CCGGACCTTTGGAGACTGTGTGTGGGGGCCTGGGCACTGACTTCTGCAAC	5	0.125	No Hit
GCCGGAGCCAGGTGCAGGCCATTGGAGAGAAGGTCTCCTTGGCCCAGGCC	5	0.125	No Hit
GCTGTGTCCATGTCAGAGCAACGGCCCAAGTCTGGGTCTGGGGGGGAAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2025-05-07T12:52:40 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open CM000668.2 as a RefSeq or as a WGS
2025-05-07T12:52:40 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra

=============================================================
An error occurred during processing.
A report was generated into the file '/home/mark.ziemann@domain.internal.burnet.edu.au/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
Read 3753620 spots for ERR5296899.sra
Written 3753620 spots for ERR5296899.sra
SRR ids: ['ERR5296899.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_zwuzw_63
ERR5296899.sra spots: 60057924
blocks: [[1, 3753620], [3753621, 7507240], [7507241, 11260860], [11260861, 15014480], [15014481, 18768100], [18768101, 22521720], [22521721, 26275340], [26275341, 30028960], [30028961, 33782580], [33782581, 37536200], [37536201, 41289820], [41289821, 45043440], [45043441, 48797060], [48797061, 52550680], [52550681, 56304300], [56304301, 60057924]]
fastq-dump error! exit code: 3
ERR5296899 file size 6273228
ERR5296899 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 16 -o ERR5296899 ERR5296899_1.fastq ERR5296899_2.fastq
Input file:	ERR5296899_1.fastq
Paired file:	ERR5296899_2.fastq
trimmed:	ERR5296899-trimmed-pair1.fastq, ERR5296899-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Wed May  7 12:55:21 2025 >> started

Wed May  7 12:55:53 2025 >> done (32.111s)
18768100 read pairs processed; of these:
       3 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
18768097 (100.00%) read pairs available; of these:
    2994 ( 0.02%) trimmed read pairs available after processing
18765103 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 29	       1	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       8	  0.00%
 34	       9	  0.00%
 35	       8	  0.00%
 36	      12	  0.00%
 37	      34	  0.00%
 38	      44	  0.00%
 39	      44	  0.00%
 40	      48	  0.00%
 41	      40	  0.00%
 42	      38	  0.00%
 43	      35	  0.00%
 44	      36	  0.00%
 45	      56	  0.00%
 46	      54	  0.00%
 47	      50	  0.00%
 48	      54	  0.00%
 49	      45	  0.00%
 50	      73	  0.00%
 51	     220	  0.00%
 52	     183	  0.00%
 53	     231	  0.00%
 54	     222	  0.00%
 55	     190	  0.00%
 56	     210	  0.00%
 57	     203	  0.00%
 58	     205	  0.00%
 59	     239	  0.00%
 60	     220	  0.00%
 61	     221	  0.00%
 62	     218	  0.00%
 63	     245	  0.00%
 64	     246	  0.00%
 65	     249	  0.00%
 66	     282	  0.00%
 67	     313	  0.00%
 68	     326	  0.00%
 69	     324	  0.00%
 70	     317	  0.00%
 71	     428	  0.00%
 72	     451	  0.00%
 73	     477	  0.00%
 74	     483	  0.00%
 75	     561	  0.00%
 76	     623	  0.00%
 77	     691	  0.00%
 78	     642	  0.00%
 79	     733	  0.00%
 80	     857	  0.00%
 81	     868	  0.00%
 82	    1036	  0.01%
 83	    1087	  0.01%
 84	    1198	  0.01%
 85	    1250	  0.01%
 86	    1537	  0.01%
 87	    1508	  0.01%
 88	    1675	  0.01%
 89	    1701	  0.01%
 90	    2028	  0.01%
 91	    2180	  0.01%
 92	    2304	  0.01%
 93	    2421	  0.01%
 94	    2614	  0.01%
 95	    2613	  0.01%
 96	    2844	  0.02%
 97	    3106	  0.02%
 98	    3274	  0.02%
 99	    3484	  0.02%
100	    3711	  0.02%
101	    3990	  0.02%
102	    4231	  0.02%
103	    4679	  0.02%
104	    4667	  0.02%
105	    4782	  0.03%
106	    5216	  0.03%
107	    5514	  0.03%
108	    5798	  0.03%
109	    5932	  0.03%
110	    6000	  0.03%
111	    6438	  0.03%
112	    6746	  0.04%
113	    7071	  0.04%
114	    7360	  0.04%
115	    7673	  0.04%
116	    8055	  0.04%
117	    8257	  0.04%
118	    8500	  0.05%
119	    8708	  0.05%
120	    9304	  0.05%
121	    9799	  0.05%
122	    9991	  0.05%
123	   10519	  0.06%
124	   10836	  0.06%
125	   11213	  0.06%
126	   11793	  0.06%
127	   12117	  0.06%
128	   12448	  0.07%
129	   13252	  0.07%
130	   13663	  0.07%
131	   14290	  0.08%
132	   14614	  0.08%
133	   15344	  0.08%
134	   16004	  0.09%
135	   16734	  0.09%
136	   17292	  0.09%
137	   17671	  0.09%
138	   18327	  0.10%
139	   19067	  0.10%
140	   19829	  0.11%
141	   20411	  0.11%
142	   21400	  0.11%
143	   22561	  0.12%
144	   24187	  0.13%
145	   25918	  0.14%
146	   23824	  0.13%
147	   24748	  0.13%
148	   25495	  0.14%
149	   26122	  0.14%
150	18085769	 96.36%
18768097 reads passed initial QC


criterion=sequence-density
sequence-density=0.50
sequence-density-rank=1
fanout-score=2.50
fanout-score-rank=37
prefix-density=0.55
prefix-fanout=2.3
sequence=TCATATTTCTTCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=150.52
fanout-score-rank=1
prefix-density=0.42
prefix-fanout=4.1
sequence=GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT


criterion=sequence-density
sequence-density=0.50
sequence-density-rank=1
fanout-score=3.91
fanout-score-rank=31
prefix-density=0.60
prefix-fanout=3.3
sequence=GCCTGTAATCCCAGCACTTTGGGAGGCC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=189.12
fanout-score-rank=1
prefix-density=0.43
prefix-fanout=4.3
sequence=GAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT
ERR5296899 testing PE reads STAR mapping to Ensembl genome
2025-05-07T12:57:24 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open CM000685.2 as a RefSeq or as a WGS
                                 Started job on |	May 07 12:56:25
                             Started mapping on |	May 07 12:56:25
                                    Finished on |	May 07 12:57:49
       Mapping speed, Million of reads per hour |	804.35

                          Number of input reads |	18768097
                      Average input read length |	298
                                    UNIQUE READS:
                   Uniquely mapped reads number |	18327986
                        Uniquely mapped reads % |	97.66%
                          Average mapped length |	297.39
                       Number of splices: Total |	16246640
            Number of splices: Annotated (sjdb) |	16030247
                       Number of splices: GT/AG |	16061162
                       Number of splices: GC/AG |	151795
                       Number of splices: AT/AC |	7559
               Number of splices: Non-canonical |	26124
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.83
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.65
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	436026
             % of reads mapped to multiple loci |	2.32%
        Number of reads mapped to too many loci |	338
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.02%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4112	4112	4112
N_multimapping	436026	436026	436026
N_noFeature	2091877	9734056	9862715
N_ambiguous	1260552	237322	234094
UnstrandedReadsAssigned:14975557 PositiveStrandReadsAssigned:8356608 NegativeStrandReadsAssigned:8231177
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
ERR5296899 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in paired-end mode
[quant] will process pair 1: ERR5296899-trimmed-pair1.fastq
                             ERR5296899-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 18,768,097 reads, 16,001,479 reads pseudoaligned
[quant] estimated average fragment length: 238.958
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,108 rounds

Read 3753624 spots for ERR5296899.sra
Written 3753624 spots for ERR5296899.sra
 180870 ERR5296899.ke.tsv
  58302 ERR5296899.se.tsv
 239172 total
==> ERR5296899.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	5.875	0	0
ENST00000448914.1	13	7.41667	0	0
ENST00000415118.1	8	4.875	0	0
ENST00000631435.1	12	6.41667	0	0
ENST00000632684.1	12	6.41667	0	0
ENST00000454908.1	17	11.4167	0	0
ENST00000390567.1	20	14.4167	0	0
ENST00000603326.1	19	13.4167	0	0
ENST00000414852.1	16	10.4167	0	0

==> ERR5296899.se.tsv <==
ENSG00000223972	0
ENSG00000227232	480
ENSG00000278267	4
ENSG00000243485	6
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
ERR5296899 completed mapping pipeline successfully
