Starting /dee2/code/volunteer_pipeline.sh SRR13211487
    current disk space = 1773797329380
    free memory = 345184652 
SRR13211487 SRAfilesize
d0651dcab450701dd91f59a86cefbc0b  SRR13211487.sra
SRR13211487.sra file validated
SRR13211487 is single end
SRR13211487 is conventional basespace
SRR13211487 read1 length is 35-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13211487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35-71
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72225	32.0	32.0	32.0	32.0	32.0
2	31.72175	32.0	32.0	32.0	32.0	32.0
3	31.77275	32.0	32.0	32.0	32.0	32.0
4	31.84675	32.0	32.0	32.0	32.0	32.0
5	31.7505	32.0	32.0	32.0	32.0	32.0
6	35.478	36.0	36.0	36.0	36.0	36.0
7	35.53825	36.0	36.0	36.0	36.0	36.0
8	35.52	36.0	36.0	36.0	36.0	36.0
9	35.593	36.0	36.0	36.0	36.0	36.0
10	35.572	36.0	36.0	36.0	36.0	36.0
11	35.5505	36.0	36.0	36.0	36.0	36.0
12	35.37825	36.0	36.0	36.0	36.0	36.0
13	35.39425	36.0	36.0	36.0	36.0	36.0
14	35.3745	36.0	36.0	36.0	36.0	36.0
15	35.30625	36.0	36.0	36.0	36.0	36.0
16	35.24725	36.0	36.0	36.0	36.0	36.0
17	35.19825	36.0	36.0	36.0	36.0	36.0
18	35.10925	36.0	36.0	36.0	36.0	36.0
19	35.37975	36.0	36.0	36.0	36.0	36.0
20	35.51075	36.0	36.0	36.0	36.0	36.0
21	35.49225	36.0	36.0	36.0	36.0	36.0
22	35.5255	36.0	36.0	36.0	36.0	36.0
23	35.39525	36.0	36.0	36.0	36.0	36.0
24	35.43625	36.0	36.0	36.0	36.0	36.0
25	35.4025	36.0	36.0	36.0	36.0	36.0
26	35.3425	36.0	36.0	36.0	36.0	36.0
27	35.046	36.0	36.0	36.0	36.0	36.0
28	34.9415	36.0	36.0	36.0	32.0	36.0
29	34.46375	36.0	36.0	36.0	32.0	36.0
30	34.77425	36.0	36.0	36.0	32.0	36.0
31	34.9195	36.0	36.0	36.0	32.0	36.0
32	35.2015	36.0	36.0	36.0	36.0	36.0
33	35.28625	36.0	36.0	36.0	36.0	36.0
34	35.313	36.0	36.0	36.0	36.0	36.0
35	35.2605	36.0	36.0	36.0	36.0	36.0
36	35.19254813703426	36.0	36.0	36.0	36.0	36.0
37	34.992994746059544	36.0	36.0	36.0	36.0	36.0
38	34.45308981736302	36.0	36.0	36.0	32.0	36.0
39	33.891864831038795	36.0	36.0	36.0	32.0	36.0
40	33.500125156445556	36.0	36.0	36.0	27.0	36.0
41	32.969704556835254	36.0	36.0	36.0	14.0	36.0
42	32.62910092662159	36.0	36.0	36.0	14.0	36.0
43	33.34134735787628	36.0	36.0	36.0	21.0	36.0
44	34.27129258517034	36.0	36.0	36.0	32.0	36.0
45	34.92935871743487	36.0	36.0	36.0	36.0	36.0
46	34.94914829659319	36.0	36.0	36.0	36.0	36.0
47	34.8812625250501	36.0	36.0	36.0	36.0	36.0
48	34.84014031571035	36.0	36.0	36.0	36.0	36.0
49	34.39383304086237	36.0	36.0	36.0	32.0	36.0
50	34.25464124435524	36.0	36.0	36.0	32.0	36.0
51	34.55385387898569	36.0	36.0	36.0	32.0	36.0
52	34.628485305199696	36.0	36.0	36.0	32.0	36.0
53	34.796282341120325	36.0	36.0	36.0	36.0	36.0
54	34.86457286432161	36.0	36.0	36.0	36.0	36.0
55	34.74893189243529	36.0	36.0	36.0	36.0	36.0
56	34.680070369439555	36.0	36.0	36.0	36.0	36.0
57	34.509927117366175	36.0	36.0	36.0	32.0	36.0
58	34.26614727318422	36.0	36.0	36.0	32.0	36.0
59	33.72900955253896	36.0	36.0	36.0	27.0	36.0
60	33.493960744841466	36.0	36.0	36.0	21.0	36.0
61	33.50276799194766	36.0	36.0	36.0	27.0	36.0
62	33.35557794006547	36.0	36.0	36.0	21.0	36.0
63	32.897151499873964	36.0	36.0	36.0	14.0	36.0
64	34.02042360060514	36.0	36.0	36.0	32.0	36.0
65	34.50100908173562	36.0	36.0	36.0	32.0	36.0
66	34.68199040161657	36.0	36.0	36.0	36.0	36.0
67	34.6	36.0	36.0	36.0	36.0	36.0
68	34.551321138211385	36.0	36.0	36.0	32.0	36.0
69	34.71075883575884	36.0	36.0	36.0	36.0	36.0
70	34.35064935064935	36.0	36.0	36.0	32.0	36.0
71	33.13406507252058	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	3.0
23	3.0
24	9.0
25	10.0
26	23.0
27	29.0
28	42.0
29	70.0
30	63.0
31	94.0
32	162.0
33	300.0
34	753.0
35	2435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.175	19.425	13.175	26.224999999999998
2	14.95	21.0	37.55	26.5
3	15.875	27.425	29.299999999999997	27.400000000000002
4	11.25	16.625	35.85	36.275
5	13.975000000000001	37.25	32.25	16.525000000000002
6	33.25820185324317	36.73929376408715	17.355371900826448	12.647132481843226
7	28.449999999999996	32.025	21.95	17.575
8	27.175	30.525000000000002	21.425	20.875
9	29.525000000000002	13.55	20.4	36.525
10	17.4	25.424999999999997	27.675	29.5
11	39.275	21.275	21.375	18.075
12	26.025	24.55	26.174999999999997	23.25
13	28.475	19.625	25.724999999999998	26.174999999999997
14	23.825	23.225	22.725	30.225
15	25.55	27.075	21.5	25.874999999999996
16	26.575	24.349999999999998	23.25	25.825
17	24.9	26.1	23.575	25.424999999999997
18	26.6	23.974999999999998	24.825	24.6
19	27.950000000000003	24.9	24.2	22.95
20	27.200000000000003	21.875	23.65	27.275
21	30.8	23.375	22.375	23.45
22	28.275	23.125	22.675	25.924999999999997
23	23.125	22.55	25.25	29.075
24	25.55	25.324999999999996	23.375	25.75
25	22.75	23.75	24.275	29.225
26	25.825	26.174999999999997	24.725	23.275000000000002
27	26.875	23.775	22.35	27.0
28	24.25	23.45	25.174999999999997	27.125
29	25.5	24.85	23.925	25.724999999999998
30	23.849999999999998	24.5	26.1	25.55
31	26.75	22.900000000000002	23.9	26.450000000000003
32	25.4	24.474999999999998	21.825	28.299999999999997
33	23.35	23.575	25.874999999999996	27.200000000000003
34	25.074999999999996	23.325000000000003	25.474999999999998	26.125
35	29.9	22.175	24.2	23.724999999999998
36	26.9567391847962	24.8062015503876	22.43060765191298	25.806451612903224
37	26.444833625218916	26.169627220415308	23.2424318238679	24.143107330497873
38	25.068801601200903	24.193144858643983	25.569176882662	25.168876657493122
39	26.733416770963704	22.453066332916144	23.57947434292866	27.23404255319149
40	27.008760951188986	24.430538172715895	25.3566958698373	23.204005006257823
41	23.69146005509642	26.34610568494866	25.519659403956922	24.442774855997996
42	29.04034076672513	26.98571786519669	22.976697569531446	20.99724379854673
43	27.3365071410674	22.776246554748184	25.056376847907792	24.830869456276623
44	24.34869739478958	25.200400801603205	22.82064128256513	27.630260521042082
45	26.20240480961924	24.39879759519038	23.196392785571142	26.20240480961924
46	25.325651302605213	25.100200400801604	25.200400801603205	24.37374749498998
47	26.152304609218437	24.19839679358717	25.0501002004008	24.599198396793586
48	29.666750187922826	24.7557003257329	22.650964670508642	22.92658481583563
49	25.595387315116568	26.422662321383804	22.3614941087992	25.620456254700425
50	25.589563472152534	26.317109884596086	23.833416959357752	24.25990968389363
51	23.273914135074065	25.53351744915893	25.684157670097918	25.508410745669092
52	25.320271288620948	23.587038432554635	23.938708867118812	27.1539814117056
53	28.811856317508163	22.532027128862094	23.913589550364232	24.74252700326551
54	28.71859296482412	24.44723618090452	23.44221105527638	23.391959798994975
55	25.6848454385524	23.950741392309627	26.011560693641616	24.352852475496356
56	27.72053279718522	23.749685850716258	22.794672028147776	25.735109323950738
57	25.182206584568988	24.227192762000502	22.090977632571	28.49962302085951
58	27.09223422970596	22.56848454385524	25.408394068861522	24.93088715757728
59	26.21920563097034	24.685771744595275	24.484665661136248	24.61035696329814
60	24.182184197282336	24.20734776044288	24.257674886763965	27.352793155510817
61	26.421741318570707	25.666834423754402	22.3704076497232	25.54101660795169
62	26.2150591790481	24.70410475950642	22.211029967262654	26.869806094182824
63	24.401310814217293	24.62818250567179	25.71212503150996	25.25838164860096
64	25.46646495209279	23.323247604639434	21.986888552697934	29.223398890569847
65	26.81634712411705	23.158425832492433	24.672048435923312	25.353178607467203
66	27.405910583480676	22.581459964637535	24.248547613033594	25.764081838848195
67	26.49810366624526	24.67762326169406	24.52591656131479	24.29835651074589
68	27.84552845528455	23.983739837398375	23.704268292682926	24.466463414634145
69	28.82016632016632	23.83056133056133	22.68711018711019	24.66216216216216
70	25.918762088974855	23.956894169660128	26.968775904946117	23.1555678364189
71	35.123480987847906	39.5531164249314	25.3234025872207	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	16.0
2	2.0
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.5
9	1.5
10	2.0
11	2.5
12	2.5
13	1.5
14	1.0
15	0.5
16	0.5
17	1.0
18	1.5
19	2.0
20	2.0
21	2.5
22	4.5
23	7.0
24	7.0
25	6.5
26	6.5
27	7.5
28	10.5
29	15.5
30	20.5
31	22.5
32	20.5
33	26.0
34	36.0
35	42.5
36	53.0
37	67.5
38	89.5
39	107.0
40	123.0
41	121.0
42	157.5
43	191.5
44	180.5
45	193.5
46	213.5
47	219.0
48	196.0
49	211.5
50	224.0
51	235.0
52	242.0
53	207.0
54	184.0
55	185.5
56	208.0
57	212.0
58	193.5
59	193.0
60	178.5
61	118.0
62	92.0
63	123.5
64	124.5
65	75.0
66	50.0
67	42.0
68	31.0
69	22.0
70	17.5
71	20.5
72	22.5
73	34.0
74	35.0
75	31.0
76	24.5
77	15.0
78	10.0
79	4.5
80	6.0
81	6.0
82	7.0
83	8.5
84	5.0
85	1.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.17500000000000002
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.025037556334501748
42	0.050087653393438514
43	0.050087653393438514
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	1.0
36	2.0
37	0.0
38	2.0
39	0.0
40	1.0
41	1.0
42	0.0
43	1.0
44	0.0
45	0.0
46	0.0
47	1.0
48	2.0
49	3.0
50	3.0
51	2.0
52	0.0
53	1.0
54	1.0
55	0.0
56	0.0
57	0.0
58	1.0
59	4.0
60	0.0
61	3.0
62	4.0
63	1.0
64	2.0
65	5.0
66	4.0
67	19.0
68	88.0
69	229.0
70	1068.0
71	2551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.0330104054539	58.550000000000004
2	7.893792608539648	11.0
3	2.978112665949049	6.225
4	1.686401148188016	4.7
5	0.8252601363473269	2.875
6	0.46645138141370646	1.95
7	0.6099748833871547	2.9749999999999996
8	0.2870470039468963	1.6
9	0.32292787944025836	2.025
>10	0.897021887334051	8.1
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	28	0.7000000000000001	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCA	19	0.475	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG	18	0.44999999999999996	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTT	17	0.42500000000000004	No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGAT	15	0.375	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT	14	0.35000000000000003	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG	14	0.35000000000000003	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	13	0.325	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA	13	0.325	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG	13	0.325	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACC	12	0.3	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT	11	0.27499999999999997	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	11	0.27499999999999997	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTAC	11	0.27499999999999997	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC	11	0.27499999999999997	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATT	11	0.27499999999999997	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA	11	0.27499999999999997	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGT	11	0.27499999999999997	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATG	11	0.27499999999999997	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGT	10	0.25	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCA	10	0.25	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG	10	0.25	No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA	10	0.25	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAG	10	0.25	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	10	0.25	No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTG	9	0.22499999999999998	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCA	9	0.22499999999999998	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA	9	0.22499999999999998	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA	9	0.22499999999999998	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT	9	0.22499999999999998	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA	9	0.22499999999999998	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGA	9	0.22499999999999998	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG	9	0.22499999999999998	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAA	9	0.22499999999999998	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGC	8	0.2	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA	8	0.2	No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCG	8	0.2	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGG	8	0.2	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTG	8	0.2	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA	8	0.2	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGA	8	0.2	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGC	8	0.2	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT	7	0.17500000000000002	No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGG	7	0.17500000000000002	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	7	0.17500000000000002	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTG	7	0.17500000000000002	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCA	7	0.17500000000000002	No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	7	0.17500000000000002	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGAC	7	0.17500000000000002	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCG	7	0.17500000000000002	No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA	7	0.17500000000000002	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCC	7	0.17500000000000002	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA	7	0.17500000000000002	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGG	7	0.17500000000000002	No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC	7	0.17500000000000002	No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG	7	0.17500000000000002	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACT	7	0.17500000000000002	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG	7	0.17500000000000002	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA	7	0.17500000000000002	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT	6	0.15	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTT	6	0.15	No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG	6	0.15	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	6	0.15	No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTAC	6	0.15	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG	6	0.15	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC	6	0.15	No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCG	6	0.15	No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT	6	0.15	No Hit
GAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGA	6	0.15	No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACA	6	0.15	No Hit
ATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTG	6	0.15	No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT	6	0.15	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGG	5	0.125	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGG	5	0.125	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTT	5	0.125	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT	5	0.125	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	5	0.125	No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGA	5	0.125	No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTT	5	0.125	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	5	0.125	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGA	5	0.125	No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG	5	0.125	No Hit
GGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTA	5	0.125	No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGA	5	0.125	No Hit
GCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGT	5	0.125	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTT	5	0.125	No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGAT	5	0.125	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT	5	0.125	No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCG	5	0.125	No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG	5	0.125	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAA	5	0.125	No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCG	5	0.125	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGG	5	0.125	No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG	5	0.125	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	40	0.003239381	32.175	1
TACATGG	40	0.003239381	32.175	2
>>END_MODULE
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30047 READS because READLEN < 1
Read 30047 spots for SRR13211487.sra
Written 30047 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
Rejected 30014 READS because READLEN < 1
Read 30014 spots for SRR13211487.sra
Written 30014 spots for SRR13211487.sra
SRR ids: ['SRR13211487.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_1u_6lzkl
SRR13211487.sra spots: 1440705
blocks: [[1, 30014], [30015, 60028], [60029, 90042], [90043, 120056], [120057, 150070], [150071, 180084], [180085, 210098], [210099, 240112], [240113, 270126], [270127, 300140], [300141, 330154], [330155, 360168], [360169, 390182], [390183, 420196], [420197, 450210], [450211, 480224], [480225, 510238], [510239, 540252], [540253, 570266], [570267, 600280], [600281, 630294], [630295, 660308], [660309, 690322], [690323, 720336], [720337, 750350], [750351, 780364], [780365, 810378], [810379, 840392], [840393, 870406], [870407, 900420], [900421, 930434], [930435, 960448], [960449, 990462], [990463, 1020476], [1020477, 1050490], [1050491, 1080504], [1080505, 1110518], [1110519, 1140532], [1140533, 1170546], [1170547, 1200560], [1200561, 1230574], [1230575, 1260588], [1260589, 1290602], [1290603, 1320616], [1320617, 1350630], [1350631, 1380644], [1380645, 1410658], [1410659, 1440705]]
SRR13211487 file size 229380
SRR13211487 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13211487 SRR13211487_1.fastq
Input file:	SRR13211487_1.fastq
trimmed:	SRR13211487-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 07:07:59 2022 >> started

Fri May  6 07:08:00 2022 >> done (1.108s)
1440705 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
     26 ( 0.00%) empty reads filtered out after trimming by size control
1440679 (100.00%) reads available; of these:
    907 ( 0.06%) trimmed reads available after processing
1439772 (99.94%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 22	     14	  0.00%
 23	     30	  0.00%
 24	     18	  0.00%
 25	     15	  0.00%
 26	     12	  0.00%
 27	      9	  0.00%
 28	      9	  0.00%
 29	     30	  0.00%
 30	     44	  0.00%
 31	    104	  0.01%
 32	    114	  0.01%
 33	     83	  0.01%
 34	    226	  0.02%
 35	    147	  0.01%
 36	    192	  0.01%
 37	    274	  0.02%
 38	    340	  0.02%
 39	    501	  0.03%
 40	    362	  0.03%
 41	    371	  0.03%
 42	    348	  0.02%
 43	    390	  0.03%
 44	    442	  0.03%
 45	    462	  0.03%
 46	    486	  0.03%
 47	    413	  0.03%
 48	    559	  0.04%
 49	    542	  0.04%
 50	    643	  0.04%
 51	    741	  0.05%
 52	    672	  0.05%
 53	    651	  0.05%
 54	    579	  0.04%
 55	    689	  0.05%
 56	    787	  0.05%
 57	    781	  0.05%
 58	    834	  0.06%
 59	    863	  0.06%
 60	    790	  0.05%
 61	    916	  0.06%
 62	    955	  0.07%
 63	    871	  0.06%
 64	    961	  0.07%
 65	   1149	  0.08%
 66	   1459	  0.10%
 67	   4650	  0.32%
 68	  27842	  1.93%
 69	  82303	  5.71%
 70	 359643	 24.96%
 71	 945363	 65.62%
1440679 reads passed initial QC


criterion=sequence-density
sequence-density=0.98
sequence-density-rank=1
fanout-score=3.71
fanout-score-rank=33
prefix-density=1.81
prefix-fanout=2.0
sequence=CCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTC


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=34
fanout-score=49.83
fanout-score-rank=1
prefix-density=5.42
prefix-fanout=1.0
sequence=AGTACATGGGCT
                                 Started job on |	May 06 07:08:32
                             Started mapping on |	May 06 07:08:32
                                    Finished on |	May 06 07:08:43
       Mapping speed, Million of reads per hour |	471.49

                          Number of input reads |	1440679
                      Average input read length |	70
                                    UNIQUE READS:
                   Uniquely mapped reads number |	352901
                        Uniquely mapped reads % |	24.50%
                          Average mapped length |	69.36
                       Number of splices: Total |	48971
            Number of splices: Annotated (sjdb) |	42102
                       Number of splices: GT/AG |	47973
                       Number of splices: GC/AG |	528
                       Number of splices: AT/AC |	38
               Number of splices: Non-canonical |	432
                      Mismatch rate per base, % |	0.56%
                         Deletion rate per base |	0.02%
                        Deletion average length |	1.20
                        Insertion rate per base |	0.04%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	953929
             % of reads mapped to multiple loci |	66.21%
        Number of reads mapped to too many loci |	19276
             % of reads mapped to too many loci |	1.34%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.84%
                     % of reads unmapped: other |	0.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	133849	133849	133849
N_multimapping	953929	953929	953929
N_noFeature	83614	215229	216274
N_ambiguous	9717	2900	2018
UnstrandedReadsAssigned:259570 PositiveStrandReadsAssigned:134772 NegativeStrandReadsAssigned:134609
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13211487 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13211487-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,440,679 reads, 415,250 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 957 rounds

 180870 SRR13211487.ke.tsv
  58302 SRR13211487.se.tsv
 239172 total
==> SRR13211487.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13211487.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13211487 completed mapping pipeline successfully
