Starting /dee2/code/volunteer_pipeline.sh SRR13210234
    current disk space = 1768961088384
    free memory = 351941136 
SRR13210234 SRAfilesize
089a343fbe3d9ff6a8be19ec51a001d3  SRR13210234.sra
SRR13210234.sra file validated
SRR13210234 is single end
SRR13210234 is conventional basespace
SRR13210234 read1 length is 35-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209
Sequences flagged as poor quality	0
Sequence length	35-71
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.842105263157894	32.0	32.0	32.0	32.0	32.0
2	30.85645933014354	32.0	32.0	32.0	32.0	32.0
3	31.43062200956938	32.0	32.0	32.0	32.0	32.0
4	30.995215311004785	32.0	32.0	32.0	32.0	32.0
5	31.401913875598087	32.0	32.0	32.0	32.0	32.0
6	34.39712918660287	36.0	36.0	36.0	32.0	36.0
7	34.645933014354064	36.0	36.0	36.0	32.0	36.0
8	34.301435406698566	36.0	36.0	36.0	32.0	36.0
9	34.78947368421053	36.0	36.0	36.0	36.0	36.0
10	34.59808612440192	36.0	36.0	36.0	32.0	36.0
11	33.95693779904306	36.0	36.0	36.0	32.0	36.0
12	34.42583732057416	36.0	36.0	36.0	32.0	36.0
13	34.133971291866025	36.0	36.0	36.0	32.0	36.0
14	34.4066985645933	36.0	36.0	36.0	32.0	36.0
15	34.143540669856456	36.0	36.0	36.0	32.0	36.0
16	33.55023923444976	36.0	36.0	36.0	21.0	36.0
17	34.430622009569376	36.0	36.0	36.0	32.0	36.0
18	33.770334928229666	36.0	36.0	36.0	21.0	36.0
19	33.95693779904306	36.0	36.0	36.0	32.0	36.0
20	33.995215311004785	36.0	36.0	36.0	32.0	36.0
21	34.28708133971292	36.0	36.0	36.0	32.0	36.0
22	34.27751196172249	36.0	36.0	36.0	32.0	36.0
23	33.92344497607655	36.0	36.0	36.0	32.0	36.0
24	34.038277511961724	36.0	36.0	36.0	32.0	36.0
25	33.5933014354067	36.0	36.0	36.0	21.0	36.0
26	33.44976076555024	36.0	36.0	36.0	21.0	36.0
27	34.08133971291866	36.0	36.0	36.0	32.0	36.0
28	34.014354066985646	36.0	36.0	36.0	32.0	36.0
29	33.50239234449761	36.0	36.0	36.0	21.0	36.0
30	33.33971291866029	36.0	36.0	36.0	21.0	36.0
31	33.50717703349282	36.0	36.0	36.0	21.0	36.0
32	33.32057416267943	36.0	36.0	36.0	21.0	36.0
33	33.88516746411483	36.0	36.0	36.0	32.0	36.0
34	33.48803827751196	36.0	36.0	36.0	21.0	36.0
35	33.10526315789474	36.0	36.0	36.0	14.0	36.0
36	32.83173076923077	36.0	36.0	36.0	14.0	36.0
37	32.90338164251208	36.0	36.0	36.0	14.0	36.0
38	32.82125603864734	36.0	32.0	36.0	14.0	36.0
39	33.57971014492754	36.0	36.0	36.0	21.0	36.0
40	32.94202898550725	36.0	36.0	36.0	21.0	36.0
41	33.41545893719807	36.0	36.0	36.0	21.0	36.0
42	33.300970873786405	36.0	36.0	36.0	21.0	36.0
43	32.70388349514563	36.0	32.0	36.0	21.0	36.0
44	32.616504854368934	36.0	32.0	36.0	14.0	36.0
45	32.58252427184466	36.0	32.0	36.0	14.0	36.0
46	33.00970873786408	36.0	36.0	36.0	14.0	36.0
47	33.74757281553398	36.0	36.0	36.0	32.0	36.0
48	33.35609756097561	36.0	36.0	36.0	21.0	36.0
49	33.0390243902439	36.0	36.0	36.0	21.0	36.0
50	34.02439024390244	36.0	36.0	36.0	32.0	36.0
51	33.0390243902439	36.0	36.0	36.0	21.0	36.0
52	33.546341463414635	36.0	36.0	36.0	21.0	36.0
53	32.97073170731707	36.0	36.0	36.0	14.0	36.0
54	33.13170731707317	36.0	36.0	36.0	21.0	36.0
55	33.28431372549019	36.0	36.0	36.0	21.0	36.0
56	33.740196078431374	36.0	36.0	36.0	32.0	36.0
57	32.583333333333336	36.0	36.0	36.0	14.0	36.0
58	32.19704433497537	36.0	32.0	36.0	14.0	36.0
59	32.783251231527096	36.0	32.0	36.0	14.0	36.0
60	32.87192118226601	36.0	36.0	36.0	14.0	36.0
61	32.59113300492611	36.0	32.0	36.0	14.0	36.0
62	32.69458128078818	36.0	36.0	36.0	14.0	36.0
63	32.80788177339902	36.0	36.0	36.0	14.0	36.0
64	32.55223880597015	36.0	32.0	36.0	14.0	36.0
65	32.81	36.0	36.0	36.0	14.0	36.0
66	31.655	36.0	32.0	36.0	14.0	36.0
67	31.67676767676768	36.0	32.0	36.0	14.0	36.0
68	32.223350253807105	36.0	32.0	36.0	14.0	36.0
69	32.364583333333336	36.0	32.0	36.0	14.0	36.0
70	31.564245810055866	36.0	32.0	36.0	14.0	36.0
71	30.68421052631579	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	2.0
27	6.0
28	8.0
29	9.0
30	8.0
31	15.0
32	23.0
33	29.0
34	50.0
35	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.49282296650718	24.880382775119617	12.918660287081341	28.708133971291865
2	14.832535885167463	30.14354066985646	30.62200956937799	24.401913875598087
3	10.526315789473683	24.401913875598087	33.014354066985646	32.057416267942585
4	11.961722488038278	14.832535885167463	35.88516746411483	37.32057416267943
5	13.397129186602871	45.45454545454545	27.27272727272727	13.875598086124402
6	34.44976076555024	36.36363636363637	15.789473684210526	13.397129186602871
7	30.62200956937799	33.49282296650718	19.617224880382775	16.267942583732058
8	26.31578947368421	40.66985645933015	12.918660287081341	20.095693779904305
9	22.48803827751196	15.789473684210526	22.48803827751196	39.23444976076555
10	17.703349282296653	28.22966507177033	32.057416267942585	22.00956937799043
11	35.406698564593306	25.837320574162682	22.00956937799043	16.74641148325359
12	22.966507177033492	27.751196172248804	26.794258373205743	22.48803827751196
13	24.880382775119617	21.052631578947366	24.401913875598087	29.665071770334926
14	19.138755980861244	23.923444976076556	24.401913875598087	32.535885167464116
15	27.751196172248804	31.100478468899524	18.181818181818183	22.966507177033492
16	24.401913875598087	36.84210526315789	19.617224880382775	19.138755980861244
17	22.00956937799043	25.837320574162682	28.708133971291865	23.444976076555022
18	21.052631578947366	27.751196172248804	29.665071770334926	21.5311004784689
19	26.31578947368421	27.27272727272727	21.5311004784689	24.880382775119617
20	24.401913875598087	22.00956937799043	27.27272727272727	26.31578947368421
21	23.444976076555022	23.923444976076556	22.966507177033492	29.665071770334926
22	27.27272727272727	22.966507177033492	27.27272727272727	22.48803827751196
23	21.052631578947366	25.837320574162682	24.401913875598087	28.708133971291865
24	30.14354066985646	25.837320574162682	21.052631578947366	22.966507177033492
25	29.665071770334926	29.1866028708134	23.923444976076556	17.22488038277512
26	27.27272727272727	23.923444976076556	22.48803827751196	26.31578947368421
27	26.31578947368421	25.358851674641148	22.966507177033492	25.358851674641148
28	27.751196172248804	25.358851674641148	22.00956937799043	24.880382775119617
29	22.00956937799043	25.837320574162682	21.052631578947366	31.100478468899524
30	26.794258373205743	21.5311004784689	28.22966507177033	23.444976076555022
31	28.708133971291865	26.31578947368421	28.708133971291865	16.267942583732058
32	27.27272727272727	26.31578947368421	28.22966507177033	18.181818181818183
33	26.31578947368421	23.444976076555022	25.837320574162682	24.401913875598087
34	22.115384615384613	26.442307692307693	26.442307692307693	25.0
35	23.076923076923077	28.365384615384613	25.48076923076923	23.076923076923077
36	26.442307692307693	24.03846153846154	25.0	24.519230769230766
37	27.053140096618357	24.637681159420293	27.536231884057973	20.77294685990338
38	35.748792270531396	25.120772946859905	23.18840579710145	15.942028985507244
39	20.77294685990338	22.705314009661837	28.502415458937197	28.019323671497588
40	24.154589371980677	29.468599033816425	26.570048309178745	19.806763285024154
41	24.637681159420293	28.502415458937197	26.570048309178745	20.28985507246377
42	20.87378640776699	28.155339805825243	27.184466019417474	23.78640776699029
43	29.126213592233007	27.184466019417474	21.844660194174757	21.844660194174757
44	21.35922330097087	29.61165048543689	19.41747572815534	29.61165048543689
45	26.699029126213592	30.097087378640776	23.78640776699029	19.41747572815534
46	21.35922330097087	31.06796116504854	23.300970873786408	24.271844660194176
47	25.24271844660194	27.184466019417474	26.21359223300971	21.35922330097087
48	28.780487804878046	24.390243902439025	23.414634146341466	23.414634146341466
49	22.926829268292686	30.73170731707317	22.439024390243905	23.902439024390244
50	25.365853658536587	23.414634146341466	22.439024390243905	28.780487804878046
51	22.439024390243905	23.414634146341466	25.365853658536587	28.780487804878046
52	27.80487804878049	26.82926829268293	22.439024390243905	22.926829268292686
53	24.390243902439025	25.853658536585368	24.390243902439025	25.365853658536587
54	23.902439024390244	26.82926829268293	27.80487804878049	21.463414634146343
55	27.450980392156865	24.019607843137255	25.98039215686275	22.54901960784314
56	25.98039215686275	25.98039215686275	25.0	23.03921568627451
57	30.392156862745097	23.03921568627451	21.07843137254902	25.49019607843137
58	27.093596059113302	22.660098522167488	23.15270935960591	27.093596059113302
59	22.167487684729064	33.497536945812804	19.21182266009852	25.12315270935961
60	27.586206896551722	27.586206896551722	23.645320197044335	21.182266009852217
61	26.108374384236456	26.108374384236456	25.615763546798032	22.167487684729064
62	26.108374384236456	25.12315270935961	25.12315270935961	23.645320197044335
63	20.19704433497537	25.615763546798032	29.064039408866993	25.12315270935961
64	23.88059701492537	25.37313432835821	21.393034825870647	29.35323383084577
65	28.499999999999996	26.0	23.5	22.0
66	22.5	27.500000000000004	27.0	23.0
67	22.727272727272727	28.78787878787879	18.68686868686869	29.797979797979796
68	21.31979695431472	26.39593908629442	25.380710659898476	26.903553299492383
69	22.395833333333336	28.125	20.3125	29.166666666666668
70	19.553072625698324	29.608938547486037	31.28491620111732	19.553072625698324
71	30.075187969924812	39.097744360902254	30.82706766917293	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.0
24	0.0
25	0.5
26	1.0
27	2.5
28	5.0
29	8.0
30	8.5
31	5.5
32	3.5
33	4.5
34	6.0
35	5.5
36	6.5
37	6.0
38	5.5
39	4.5
40	4.5
41	7.5
42	5.5
43	6.0
44	8.5
45	8.0
46	9.0
47	8.5
48	8.0
49	8.5
50	7.0
51	6.0
52	6.5
53	8.0
54	7.5
55	7.0
56	7.5
57	7.5
58	7.5
59	6.5
60	8.0
61	9.0
62	9.0
63	9.0
64	8.5
65	6.0
66	4.0
67	4.5
68	2.5
69	1.5
70	2.0
71	1.5
72	1.0
73	0.5
74	0.0
75	0.5
76	1.0
77	0.5
78	0.5
79	1.0
80	0.5
81	0.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.4784688995215311
35	0.4784688995215311
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	1.0
36	1.0
37	0.0
38	0.0
39	0.0
40	0.0
41	1.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	1.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	1.0
55	0.0
56	0.0
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	2.0
64	1.0
65	0.0
66	2.0
67	1.0
68	5.0
69	13.0
70	46.0
71	133.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.60765550239235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.54901960784314	95.21531100478468
2	2.450980392156863	4.784688995215311
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCAGTGAAATCCCAGTGTTGTTCAGGGGAGATCTTCAGTTTTCCTGATGACTCAATGCTGTGTGTTCTCAG	2	0.9569377990430622	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAA	2	0.9569377990430622	No Hit
GCCGAAGCAACAGCGTTTCCCGTTGAGGCTGTGCGATGGCAAGAAAGTATTACGCTCAACGCATGTCTC	2	0.9569377990430622	No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG	2	0.9569377990430622	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.9569377990430622	No Hit
CCCTGGCGAGCCCATGAGTGCACGCCCTCTGGCTGCAGAGCAGAGGATGAGGCACAGAGAGGGAGCCCCAG	1	0.4784688995215311	No Hit
GCCTAAAAGGCAGTGGCCTTAGGCAGAATAGGGTGAAACAAAGAGTAATTCAAATAGAAAGCTGCACCCTT	1	0.4784688995215311	No Hit
CTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGCTCTGGAA	1	0.4784688995215311	No Hit
TATCTTCAGCATGCATCAGATGTTCCTGGAGGATTTGTTTTCTGTTTTAGGAGACAGGGTCTTGCTCTGTT	1	0.4784688995215311	No Hit
CCTGAAGCCTCTCTTCCTGGCTTCAGACCCTCACTGAGAACCCAGCTGCTGCTTCTGGAAACATCCAAATA	1	0.4784688995215311	No Hit
AAATTCACATCACCCACCATATAGGGCTTGCATTACCACGAGGCAGAAAGCACCTAGTGTTGCTGCATCTT	1	0.4784688995215311	No Hit
GCCCAGAGCAAGAGAGGCATCCTCACCCTGAAGTACCCCATCGAGCACGGCATCGTCACCAACTGGGACGA	1	0.4784688995215311	No Hit
CATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCG	1	0.4784688995215311	No Hit
GAGCTGAAATTGTGGCAATAATCAACAGCTTACCAACGAAAAAGAGTCCAGGACCAGATGGATTCACAG	1	0.4784688995215311	No Hit
GATATTACTGACTCTAGGACTGGGATTAAAAAAATGTTTAAACCAAATGAAATTACAAATTATTGCACAAA	1	0.4784688995215311	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA	1	0.4784688995215311	No Hit
CTATAGAATTTGTTTATGCTTTGCTTGATGGTTTGCTATCAAATCTGAACGTAGGGAAATGTTTAAATGTT	1	0.4784688995215311	No Hit
GTACATGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT	1	0.4784688995215311	No Hit
CTATAATATTTTGAAAAAAATCGCATATTAATCGGACTTTTAAAGTCGTACTTAATTGCTTCACATTCAT	1	0.4784688995215311	No Hit
CCCCAAAGGGGTTCTCTGGGCTGTGCCTCCTTTCCTTGGTTCACATGGGAGGGGGGGTGCCGGGTTGATGA	1	0.4784688995215311	No Hit
AGTGACCTCGGTAAGGACGTCATTAGGGATCACCCCCTGGCCGATCTCTCGGACGCACACGGCCTGAACAG	1	0.4784688995215311	No Hit
AACCTATACCGACCATTTCTCCGGCACCCTCAAAGGTATAAGCGATAACCTCGATCACATCTCCGACATGG	1	0.4784688995215311	No Hit
CAATACAGAAGGCCAACCTGCTGTAACTACTGGTGAGCAAACTGGTGGTAAGCCTGCTGCAACGAAAC	1	0.4784688995215311	No Hit
AGGCAATTTAGGGGAAAAATCTGGTCTCCAAGCACAGATAACACTCTACTCTTGAAAGAGGAGACCTGCT	1	0.4784688995215311	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAGAGGAATCATCGAAG	1	0.4784688995215311	No Hit
CTCCCGGACAGTGCGGCACTCAGCACCCAGGTAGTTGCGCAAGTTGACAGCGTGGATGGCAGAGCAAGCTT	1	0.4784688995215311	No Hit
GTATCACCGCAGAGTACATGGGTACCTGGTGGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	1	0.4784688995215311	No Hit
CCTAATGGCATAGTAACGCTGATCATATCAGCTCCCCACTGCCAGGGCTATGCTGACCCATGCCAACCAAG	1	0.4784688995215311	No Hit
CTGCTAAAGAGTATGGAGATTTTTAGGGGCAATGAAAATACAAAAATACAGATTTTGGTAATGGTTGCACA	1	0.4784688995215311	No Hit
ATCCGGTACCCGTCGCGAGGAATTGCTGCTGTCGCGCGAGGAGACAGCGATTATGTGGAAGCTGCGCAGAG	1	0.4784688995215311	No Hit
CTTCAAGAGTTTGACAGAGATTTAGGTCACTGTGGCAGCGTAAGCCTCTTACCACTGTTTCTCATCTTG	1	0.4784688995215311	No Hit
TTTTAGCTCTTTGAGGAATGTCATAAAGATTTCCAGAATGGTTAAACTAATATACAC	1	0.4784688995215311	No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACAACCTCCCCGGGTCGGGAGTGGGTAATT	1	0.4784688995215311	No Hit
AATTACAGCTGATCTGAATTTCTTTGTGACATCTAAACACTTCCCAGTAAGTCTGTTCTGGGACTCATTC	1	0.4784688995215311	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCA	1	0.4784688995215311	No Hit
TAACAATACAGGACTCTTTCGAGGCCATGTAATTGGAATGAGTCCACTATAAATCCTTTAACTAGGATCAA	1	0.4784688995215311	No Hit
CATAAGACTGGGTGATTTATCAAGAAAAGAGGTTTAATTGGCTAATTGTTCCACAGGCTGTACAGGAAGC	1	0.4784688995215311	No Hit
GATGAGAGGATTCAGGCAACAATGGCTAAATGCAACCGTCTCAGTCACACTGAGGGCCAGCCTCAGATC	1	0.4784688995215311	No Hit
CATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTAAGAAACCGGCATGC	1	0.4784688995215311	No Hit
TATCTGACGTCTGTGTGAATCCATTTGGTGGTGGTCTGGGTTTCTGAAAAGTAGCTCAGGGGCACGTGTTA	1	0.4784688995215311	No Hit
GAGCAAGAAAATCGCACTTTTTTTCTGTTAATAGATTTTATTAGTGGGAAAAACTGTTTTACACACCTAT	1	0.4784688995215311	No Hit
CTCCAGGAATGGCCCCCATCCACGCTCTCGGCGGTCAGCACCTTCCCACCGGAAGAAGCGGAGTGTCGGTT	1	0.4784688995215311	No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTGCG	1	0.4784688995215311	No Hit
CCATGGTGACCCGCGTTCTCGACGACAAGGGTCACCAGGTGTGGACCGGAACGTTCGACGAGTCGAAGCT	1	0.4784688995215311	No Hit
AAACACTACACTTCAGCTGCCAAGGCTGTTTCTCACAGGTACAAGAGGACATCATTACAGATCCTACAGA	1	0.4784688995215311	No Hit
GTTGGCGCCGGCGGGATTGGTGTGTTATTGTGGGAAGCAATCCGGGGCTTTGCCTTCGGACAGACAGCGG	1	0.4784688995215311	No Hit
GTGTGTGATGTAATAAATAGTACATGCGCCTGAATGTTTTTCTAAAATCTAGAAACATCTGAATTCAAAAG	1	0.4784688995215311	No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGCGGCGAGCCACCGCCCGTCC	1	0.4784688995215311	No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAA	1	0.4784688995215311	No Hit
GGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTC	1	0.4784688995215311	No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAA	1	0.4784688995215311	No Hit
GGGTCGTTCTCCAGACGCCAAGCACCGTAGGCCNN	1	0.4784688995215311	No Hit
CCTCTGTATCCGCTGCTGAACGTGCCGCACGAAGGCGGCGAACCTTCCTTGAGCCCGGAGGCGCGGGTGAA	1	0.4784688995215311	No Hit
GATTCAGAATGGAATGGAATGGAATGGATTTAGAATGGAATGTAATGGAATGGAGTGAAGTGTAGTGGATT	1	0.4784688995215311	No Hit
CTATATGCATAAACAAGCAGACAAGCAAAAAAGAAACTAATAAAAACCCTAATATCTGAAGTTTATTCC	1	0.4784688995215311	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1	0.4784688995215311	No Hit
GTACATGGGGCAATGGCACCAGCACGGGCAGCAGCTGGCACCTTATTGGCCAGCAACATGTCCCTGATCT	1	0.4784688995215311	No Hit
GGTTAGTGGACTGTGAGTCCTACGAAGGGTTATTAGGAAAACCGACGACGAGACATGGGAACCAACTATAA	1	0.4784688995215311	No Hit
GTTTTGTTCTGTAAAATAAAATTTAAGTGTACCTGCTACTTCATTATGAGTATATAGAAGTACAACGATGG	1	0.4784688995215311	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCAGTCTTCTGCTTGAAAAAAAAAT	1	0.4784688995215311	TruSeq Adapter, Index 6 (96% over 27bp)
GATCTAGCCTTCGCTATCCACGGCACGTCGGAGCTTTCCTTCGCAGGTGCTGCGGGGGCCCTGTTCTTCGG	1	0.4784688995215311	No Hit
CTCCCGACCCGGGGAGGTAGTGACGATAAATAACAATACACGACTCTTTCGAGTCCCAGTTATTTAAATGA	1	0.4784688995215311	No Hit
AACGAAGACTAGCCAAAAAAATGGGCAATTATTTCACCTGAAATGAAATTATCTGCTAAAATTAACACAAT	1	0.4784688995215311	No Hit
GCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCT	1	0.4784688995215311	No Hit
TGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT	1	0.4784688995215311	No Hit
GATCTTGGGAGCGGGTGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCG	1	0.4784688995215311	No Hit
ACCCATAACGGAGGCACATCATGGACACGCTAATGCGGATCACCGACAACTTGACAAACTCGCCGGGTATA	1	0.4784688995215311	No Hit
ATTCGATTCCATTCTATTGGATTGCATTACATTCGTGTTCATTCCATTCCAGACCATTCCATTTGACTCCA	1	0.4784688995215311	No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTA	1	0.4784688995215311	No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGG	1	0.4784688995215311	No Hit
GGTAATAGCTCTACCATTTATATTTAGCAAAAGGTCAAAGAATATTTGGCAGATCCTCATGGTAGACATTT	1	0.4784688995215311	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTAC	1	0.4784688995215311	No Hit
ACCATGTTCCGTCAGATAGGAGACCCGCCGGAGTGGATCCTCGAAGCGGCAAGGAGGAGTTACGACGTAC	1	0.4784688995215311	No Hit
GGCAGTCGGTTGACTATGGCCGTATAACTTTCGTTGATATGACCGGCTCTATTACGCAGGGTCAAAACGAT	1	0.4784688995215311	No Hit
GAATGCAAACACCTCAAAGTGAAAATCATCACATGTAACTATAAATCATCATTTGCACAGACAGTGGA	1	0.4784688995215311	No Hit
GGTCAGCGTCGGCCACCAGGGTGGTGTGGCCCTGCTTGGCGGCGGCTGCCGCCAGCAGGAGGG	1	0.4784688995215311	No Hit
CATTAGATGACATACATTCATCACAGACACCCATTGCCCACCACTACTACCATAAACCAAACTGCCATGGA	1	0.4784688995215311	No Hit
AACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAA	1	0.4784688995215311	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTATCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	1	0.4784688995215311	No Hit
TCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACG	1	0.4784688995215311	No Hit
CTCAACGCCAGTCTGGACCCCCTGCTCTTCTATTTCTCTTCTTCAGTGGTGCGCAGGGCTTTTTGGAGAGG	1	0.4784688995215311	No Hit
AAACTTCCTTGTGATGTGTGCATTCATCTCATAGAGTTGAACTTTTCTTTTGATTGAGCAGTTTGC	1	0.4784688995215311	No Hit
TACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTCG	1	0.4784688995215311	No Hit
TTCTTGTTCTGCCGCTTGTGAGCTGGTGATGTTATTTGTATATCACTTTGCTTCTCTGAACCTCCAGTTT	1	0.4784688995215311	No Hit
ACTCAGGATTTAAAAACTGGAACGGTGAAGGTGACAGCAGTCGGTTGGAGCGAACATCCCCCAAAGTTCA	1	0.4784688995215311	No Hit
GTATGAATCAAGGGACATGTTAATTATAAGAATGGATAGAATTTCAACAGGGAGCTTGGATAGGCAACAAA	1	0.4784688995215311	No Hit
AAAGGAGATTGGAAGGCCCCAGAAGCCACCTCTCTCTGGCCTTCTCCTGCCCTGTGATCTCTTGCCCCTAT	1	0.4784688995215311	No Hit
CCTCATCACTTACAGGGAGTGGAAATGGGCCACAGATCTCTCTATCCCCATGAAGTCCCTTCCAGGAATCA	1	0.4784688995215311	No Hit
GTATTGGTGATTTCACTCAATATGAAAACAGGTTGTTTTTGAGGATTAAGTACAAAATAAAAGATGACTGA	1	0.4784688995215311	No Hit
TGTCAGAACTCACCAATTCAACCCATCGCTGCACTTATCACGTCGAGCCACTAGGTCGATACAAATCTTAG	1	0.4784688995215311	No Hit
CTTCTAAAGGTCATCCCTCTCTGGGCCAGGCGTGGTGGCTCATGCCCTTAAATCTCATTTTTAAGAAACC	1	0.4784688995215311	No Hit
GCCGACGTGGTGCGTCGCCGCTACGGACTCGTCGAT	1	0.4784688995215311	No Hit
CTTCTTGATAATCCAGTCATGTTGGTGTAATTCTACAAATTTAACAC	1	0.4784688995215311	No Hit
CGCTTGCCCCATCGATCGCCGGAGCATCGCTTGCTACCCTTGACTTGCTCAAATCCTCCGGCGCCCTGCT	1	0.4784688995215311	No Hit
ATTATGGATGTGAGACAATCAGAAAACAGTTATGAGGGAAGGTGCAAGAGAAATGGGGTTTATTCCATAG	1	0.4784688995215311	No Hit
GACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGG	1	0.4784688995215311	No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGGACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1	0.4784688995215311	No Hit
CTATCGCAGCAGCATCCGTTGGTCTTTTTTAATGCAAGAAATCCGCCCATCCTTCCTTCCTTCTTTCCTT	1	0.4784688995215311	No Hit
CTCTAGAGATGATGCAATACATCTGAAAACTTATCAAAGTCTACTAATATCAATATTTTGGCACTTCAAAT	1	0.4784688995215311	No Hit
GAATTGTGGAGTTGGGTGGTGTTCTGTGGTTAGCAGGTCTATGCGAACCTACCCCTAAAAGTCCAAGGAAG	1	0.4784688995215311	No Hit
CATCTTTATTGATCACTTACTAAGGACCAGAAACTACTCTCAGTGCTTCACATTTAATAACTTATTTAAT	1	0.4784688995215311	No Hit
ACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCA	1	0.4784688995215311	No Hit
GTCCAATGCACCGCTTGTCAATGTGACCCTCTACTATGAAGCACTGTGCGGTGGCTGCCGAGCCTTCCTGA	1	0.4784688995215311	No Hit
CCGCCCTGGTGCCGTCAGCTCCAGGGCTCGTCGCCCAGATCGCCAAAGTCAGCCCCCACACCAGTCCAGTT	1	0.4784688995215311	No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGCTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT	1	0.4784688995215311	No Hit
CACCGGTATGGGGTGCGCAAAGCTGGTGCACGGGGTATGCGAGGGATGCGACATTGCCACCAATGCCG	1	0.4784688995215311	No Hit
ACCATACGTAGTAGTTACCGTAAGGACCATCCGGATCGGCGCGTGAGGACTGGAACCACGGATGGGAATC	1	0.4784688995215311	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCGGAGAAACGGCTA	1	0.4784688995215311	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTG	1	0.4784688995215311	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATG	1	0.4784688995215311	RNA PCR Primer, Index 44 (100% over 29bp)
ATCGCATACATATCGAGGGACCCGTCGCTGGAAAGATGCTAGGCCTGCTTGAAT	1	0.4784688995215311	No Hit
GCTCAGAGTTGATAAACATTTCCATATCTGAGCAGGAGAGGTAAGTTTTATCTATTAAGTGTTGCAGTCAA	1	0.4784688995215311	No Hit
GTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCTCCAATTTTTCAATTCTCTCT	1	0.4784688995215311	No Hit
TATTTGTTCTTGCCTAAAATGCTTTTTTCTCTGGGTGGCGTTATCCATCCCTTTCTTGTGTCTGTCAGACA	1	0.4784688995215311	No Hit
ACTTATAATAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAATT	1	0.4784688995215311	No Hit
GATCTCCTTCTGCATCCTGTCGGCAATGCCAGGGTACATGGTGGTGCCGCCAGACAGCACTGTGTTGGCGT	1	0.4784688995215311	No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4784688995215311	No Hit
GCATATAACAGTATAATGGATATGGATATGGACAAAGTACATAAAACAAACATTAGTCAATGTAAAAAGCA	1	0.4784688995215311	No Hit
CTCCAAGATGAACATAATAGTGCGTTTGCTAGAATCCCTCCTGTTCAGCAAAAATACATCCGATGGACCCA	1	0.4784688995215311	No Hit
ACATTAAATATTCTCTCAAATTCCTTGAGGCATAGCAACTTGAGCTTACCAGAAAAGTGCTGGGATTATAG	1	0.4784688995215311	No Hit
GTTATCGAAGTCATTTTTCGAATCGCCGTAGGGGTTGAGCGGGCGTAGCAGCGGCAGCAGCGCATCGTCGT	1	0.4784688995215311	No Hit
GAGATAGGTGATTCCCAAAGCACGGTCATATAGCGGGTAACCAGGACGACCCGTTTCACCCCAGATCATC	1	0.4784688995215311	No Hit
ACCACCACTATCACCACTACTGATAATACTATTACTACTACTACCACCACCACCACTGCTACTG	1	0.4784688995215311	No Hit
GGTCTAAGGCTAGTACTCCTCCACAGCCAATGCAGTGCTACCTTCATAGACTGTGTCTATGCTGCATCTTA	1	0.4784688995215311	No Hit
GACCAAGGCAAATTAAATGAGAAAGCATTTAAATAGGGGCTAGAATACTTTGTGTCAGTATGGCGGCAGG	1	0.4784688995215311	No Hit
GCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTC	1	0.4784688995215311	No Hit
CCTACGGAACGCTCCGCTACCGCGTGATCCTTGCAGATCACACCCAAAGCTTCGGCTCGTGGCTTGAGCCA	1	0.4784688995215311	No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTAT	1	0.4784688995215311	No Hit
ATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCA	1	0.4784688995215311	No Hit
CATTAAGTCCTAGGGTACATGTGCACAACGTGCAGGTTTGTCACATATGTATACATGTGCCATGTTGGTTT	1	0.4784688995215311	No Hit
TGTCTGACCTCCCATCCCATCCTGGCTGGGAACTCAGTTTTTTAAGGATTCTCTAGGGTTACCTTGGCCAA	1	0.4784688995215311	No Hit
GTCTCGCTCCGTGGCCTTAGCGGTGCTCGGGCTACTCTCTCTTTCTGGCCTGGTGGCTCTCCACCGTACT	1	0.4784688995215311	No Hit
CTATTGTTTTTATACAGTCCCTTTTGCTTTAGTCTGACATTTACAGTAAGAATCCTGTTTTACTTTCACTT	1	0.4784688995215311	No Hit
AAACTAAATATATTCTTTACCATACAAGTAAGCAATTTTGTTTCTTTGAATTTATCCACATGAATTGAAAA	1	0.4784688995215311	No Hit
GAAGAATCCGTCCTCAACGGCGACGAAGTAGCCACCCACGTGCAGCATCTCGGTGAGGAAACCGATCGCC	1	0.4784688995215311	No Hit
GGTTTGGATGCAGAGTTTTGAGAGAAGGGTGATCAGAGCTAGGGTCTAAGCAACCTTGGTCTGCTCCCTTG	1	0.4784688995215311	No Hit
CTCCTAACGTCATGCCAAGTTTACATATGCCGCTGCAGTCCGGTTCCGACGTTGTGTTGCGTCGGATGCG	1	0.4784688995215311	No Hit
CACTGGAGGTACCTCAGATCGCTTGGCAACTGGATCGGTCCCGCCGGTCGTGTCACACAGTCTGTGTAGGT	1	0.4784688995215311	No Hit
AATTACAGGGCCTCGAAAGAGTGCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAA	1	0.4784688995215311	No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT	1	0.4784688995215311	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTC	1	0.4784688995215311	No Hit
CCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACAACATTTATACAAGTAGGAGAGG	1	0.4784688995215311	No Hit
TTCTCGTACTGCTTCTCTACTTTGGCTTCGCTGCTACTGGAACTGTATTGGCTGGACAAGCTATCAACAAT	1	0.4784688995215311	No Hit
ACTGAAAATAATGAGGAGTACTAATTGAATTGATTCCCCAGAGTTTTTTGTTGCTGTGGTGGTTGTTTGTT	1	0.4784688995215311	No Hit
GAACCAGACACTCCAGTAACAACAGTCATCACTGATAATGGAAAATCAACATCTACATTTTTGAGATTATT	1	0.4784688995215311	No Hit
CATATTGTCAGAGCCAAGTCAAGATGAAAAGAATTGGTACCATACGACTGTGGAAGCGCATGAGAACTTTG	1	0.4784688995215311	No Hit
CCTCAATCCCTTACCTTAGTAATACTGGACTTTAATGACTGGTAATGACCTGTAAAACCAAGTCAACAAAT	1	0.4784688995215311	No Hit
GCACCTAGGCTGTGTGAGAAAGCTGGCTAAGGATTTGTGCCTGGGAGACCCATGGACCATGTTTCTTGCAG	1	0.4784688995215311	No Hit
ATTTGAAACATGGAAAATCAGACTAACCCTTCCGGCTACCAAAAAAAAAAAAAAGAATAAAGTATGAAAG	1	0.4784688995215311	No Hit
TCATCGAGCGGATGGCTCCGGTCGAGAAGGTGCGTTTCGTCTCAACAGGCACTGAGGCGACAATGACTGCG	1	0.4784688995215311	No Hit
GCCAACCTAGGCCCTGGTTTGGACAAACCCGGGCCTAGGTTGGCTTTGCCACGCGAACCAGGCCCACCGCA	1	0.4784688995215311	No Hit
ACATAGGACCACTTAACTTTGGGGAGGGAATGTCAGAGGCTTTATTCAGATTTCAAAAAAAGGAGAAAGAA	1	0.4784688995215311	No Hit
TATACATAGCATGCTATCAACTTATGAGCCTGATTTTTATTGTAAGCTTCCCACTACCTTCCCTACTTGTA	1	0.4784688995215311	No Hit
TTTTCAAAGTAAACGCTTCGGGCCCCGCGGGCCACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAG	1	0.4784688995215311	No Hit
CGTTATAACTGATGTATACCCCAGGTGCTATAGGCCTGTAGTGGTAGGAGGTACTGAGTTTTGAAGGATAA	1	0.4784688995215311	No Hit
CTCTAAGACCAAATAACCCTAGAATGCATGCTTTCACAAAATGAATGGTCTCAACATACAATTTAACCCAA	1	0.4784688995215311	No Hit
TCTCAGTTGGGCACAGTCCAACCCCTCTCGGAGCGCCTCGAAACGGCGTCATCCCGGTTCCCCAGTCTCTT	1	0.4784688995215311	No Hit
AGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGA	1	0.4784688995215311	No Hit
TCCATATGGAATTATAATGCTGTTTTTCCTTCTCAGAATAA	1	0.4784688995215311	No Hit
GTGCCTCGACGTCCTTTTCCTACCTTCACTGCGTATGGGGGAGTGGGTTGACGTCGGTGGTCGAGTGAACA	1	0.4784688995215311	No Hit
CTTTACTTCCAACTATGTGGTCAATTTTGGAATAGTTGTGGTGTGGTACTGAAAAGAATGTATATTC	1	0.4784688995215311	No Hit
CTCATGACGTGTCCACCGGAGTCGACATCCACACCACCCGTCAGCCGGTCGGTGTCGTCGCGGGTATCTG	1	0.4784688995215311	No Hit
AATTTAAGCTGCCAGTCAAGCAGATATGGTATTACATAACACATTTTTATGACCAAGTAGATATAGTCAC	1	0.4784688995215311	No Hit
CTTCCACCCCACTTCTTCCTGCCCCAACATGCAAGGTCTTTACTACCCTGCCCGCCAGATAGATAGACAGT	1	0.4784688995215311	No Hit
CGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA	1	0.4784688995215311	No Hit
AATTGGAAACATCATTTGCCCACCTCTATTCTGCTGTGTGCCCTGAGCAGCTGATGTGCACAGATGGCAT	1	0.4784688995215311	No Hit
TCCCAGTACAACTCCGTCGAACCGCCTTGTGCCCCCCGAAATCTCGCATTGGCCATCGGGAAACGCCTCA	1	0.4784688995215311	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGA	1	0.4784688995215311	No Hit
GCCATTGGCTGGCTGGAATTCCAAGCCAGTGTGTCTTAACTTTTGAGGTGCCATGGGAAGTGGGGCCTGCA	1	0.4784688995215311	No Hit
GCCCATGACCAAGGAGGAGTGGGATGCCCGGCAGAGCATCATCCGCAAGGTGGTGTACCCTGAGACGGGG	1	0.4784688995215311	No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCAGCGGGACA	1	0.4784688995215311	No Hit
GTCTCAAACATGATCTGGGTCGTCTCCCCCCGGCGCCCCAAGGCCACCCGCGAGTTGGTGCCCCAGAGA	1	0.4784688995215311	No Hit
CTATAGAATTTGTTTATGCTTTGCTTGCTGGTTTGCTATCACATCTGAACGTAGGGAAATGTTTAAATGTG	1	0.4784688995215311	No Hit
ATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGAGCCGGAGAGGGGCTGACCGGGTTGGTTT	1	0.4784688995215311	No Hit
AACGAAGACTAGCCAAAAAAATGGGCAATTATTTCACATGAAATGAAATTATCTGCTAAAATAAACACAAA	1	0.4784688995215311	No Hit
GAATGTACACAGAACAAAAAATCATCTTGATTAGTTTTAAGAAAAAGTACTGGCACTTTGGAACGTGGTAA	1	0.4784688995215311	No Hit
CATCAGCAGGACTAGCGTCTTGTCAGTCTTGGTGACAGTGACATTGAAGGTGGGGGCCCCACCGGTGCT	1	0.4784688995215311	No Hit
ATCTCGGTTTGCATGATCTCAGCGTCGGCGGGAACGTCAGAGAGCACCGTCGGTGGATAGAACCAGCCCCG	1	0.4784688995215311	No Hit
GACCCAGCTGGAGCAGTGGCTCAAGGAGCGGGAGAGCGGTGAGCGCGACTTCGTGCTGATCGACGTCCGCG	1	0.4784688995215311	No Hit
TGGGTGAACAGACAGCAGGAGCCGTCGCCGCTGAATGGGGCCGATCGGCGAATGACGACAAATGAATGT	1	0.4784688995215311	No Hit
AGGTTCCGCCGGCCAAAGTCTTCGTCATCGGTAATGGCGTCGCCGGTTTGGCTGCACTGGGCGCCGCCAAT	1	0.4784688995215311	No Hit
GAACTGCAGCAGCTCTGGAAAGAAGTGACAAGTGCTCATACTGCAGAAGAAACCACAGGCTACAATCACAG	1	0.4784688995215311	No Hit
GTGAAACCTCGTCTCCAATAAAAATACAAAAATTAGCCAAGAGTGATGGTGCCCACCGGTAATCCCAGCTA	1	0.4784688995215311	No Hit
AATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGAGCGGGCGGTCCG	1	0.4784688995215311	No Hit
TAATTGGCTTAAGAAACAAAGACAAAAATAAATCAGGCCACGAATCAGGAAAGTATAACATAAAAATAATG	1	0.4784688995215311	No Hit
TGATGCAGGTGCCCAGGCCCTTCGACGGCTTCGTGGAGCACACCAAGCGCGTCTCACCCACCTGCCTGGTG	1	0.4784688995215311	No Hit
CGGTGGCCCATCGGATGGCCGAGATCAGCCGCGACGTCATCGAGGAACGCACCGGTATGCGGATCAGTGA	1	0.4784688995215311	No Hit
TTATTATTTTGAGAAAGGGTCTCACTCTGTTGCCCGGGCTGGAGTGCAGTGGCACGATCATAGCTCACTG	1	0.4784688995215311	No Hit
CAATAGCGCGACCTGCTGGGCGACCGTTGTAGCCGCCTTGAGCGGCTTGGGCATTGCACGGCTCCTCGGG	1	0.4784688995215311	No Hit
GTCCAGCCAAAATCGGCACTGTCGGCCGGTTCGGCAGACAGCGAGCAGCCCAGGCGACAGCTTCGGCCATG	1	0.4784688995215311	No Hit
GCCCAAGACATCTCCCCCATGCAGTGGCGGATGATCGGACGTCGAGGCCGCCAGGCTTCCTGGACGATCGT	1	0.4784688995215311	No Hit
AGATAATGATTAATATCATTGAAAAGTGCTTGGCACTTGAATGAGTCATAAATAATCTCCTTTGAACAAAT	1	0.4784688995215311	No Hit
AGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTT	1	0.4784688995215311	No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACTTT	1	0.4784688995215311	No Hit
GGGAGTGGCTGAAGGACTGTACTGGCAGAGCACCCAAGGAGCATTTGGACTGTCTTCTCATCTCTCCCTTT	1	0.4784688995215311	No Hit
CACCGGGATCGGGCGGCATCCCCCGGAACTTTGACATGTTTTCCCGGCTGCGCGGCTGGCAGCCCCATTTT	1	0.4784688995215311	No Hit
CCTCCTACCGCGAAGATACCATCGGTTCTTGTGTATTGGTGACACAAGAAGGTACTCGTCCATTATTAATT	1	0.4784688995215311	No Hit
GCATTAGGTTGTCCAGTTTGTTCTCCATTAGTATTTGGCGTACCAGTCACAGCTGGGTAATTATTAGGTTT	1	0.4784688995215311	No Hit
ACAGTCACTTAATTTTACTTGTAAACAAAATTTTTGCAAAAAGTTATGTCTTTAATTCTCTTCCTAATTTT	1	0.4784688995215311	No Hit
TAACCCAAAGCACCCTTACACCTGGGGGCTTCTCGGCTCTATGCCGACGGTGGATCATCGCGAGGAAGAAT	1	0.4784688995215311	No Hit
GACCCCGCCGAAGGTTTTCTCCGGTTCGGGAGACAGTTAGGTAGTGTTGCGAGCGAACAGATTTGCACAGA	1	0.4784688995215311	No Hit
CCGCAGGGTATTATGCAACGGGCTATAACACTCCCGGAGGAGCCACGTTCCCGAAGCCTTTTTCCCCCGCG	1	0.4784688995215311	No Hit
CATGGGTAGAGAGTAGAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCA	1	0.4784688995215311	No Hit
CTTCTCCCACAGGTAATCACCGGTGGGGATTCCCTCGATCTTGCGATCCATCGTCATCTCGAACAGGATCG	1	0.4784688995215311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210234.sra
Written 4 spots for SRR13210234.sra
Rejected 21 READS because READLEN < 1
Read 21 spots for SRR13210234.sra
Written 21 spots for SRR13210234.sra
SRR ids: ['SRR13210234.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_wz4b_q3y
SRR13210234.sra spots: 209
blocks: [[1, 4], [5, 8], [9, 12], [13, 16], [17, 20], [21, 24], [25, 28], [29, 32], [33, 36], [37, 40], [41, 44], [45, 48], [49, 52], [53, 56], [57, 60], [61, 64], [65, 68], [69, 72], [73, 76], [77, 80], [81, 84], [85, 88], [89, 92], [93, 96], [97, 100], [101, 104], [105, 108], [109, 112], [113, 116], [117, 120], [121, 124], [125, 128], [129, 132], [133, 136], [137, 140], [141, 144], [145, 148], [149, 152], [153, 156], [157, 160], [161, 164], [165, 168], [169, 172], [173, 176], [177, 180], [181, 184], [185, 188], [189, 209]]
SRR13210234 file size 1
SRR13210234 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13210234 SRR13210234_1.fastq
Input file:	SRR13210234_1.fastq
trimmed:	SRR13210234-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 14:28:32 2022 >> started

Fri May  6 14:28:32 2022 >> done (0.005s)
209 reads processed; of these:
  0 ( 0.00%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
209 (100.00%) reads available; of these:
  1 ( 0.48%) trimmed reads available after processing
208 (99.52%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 33	  1	  0.48%
 34	  0	  0.00%
 35	  0	  0.00%
 36	  1	  0.48%
 37	  0	  0.00%
 38	  0	  0.00%
 39	  0	  0.00%
 40	  0	  0.00%
 41	  1	  0.48%
 42	  0	  0.00%
 43	  0	  0.00%
 44	  0	  0.00%
 45	  0	  0.00%
 46	  0	  0.00%
 47	  1	  0.48%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  1	  0.48%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  1	  0.48%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  2	  0.96%
 64	  1	  0.48%
 65	  0	  0.00%
 66	  2	  0.96%
 67	  1	  0.48%
 68	  5	  2.39%
 69	 13	  6.22%
 70	 46	 22.01%
 71	133	 63.64%
209 reads passed initial QC
                                 Started job on |	May 06 14:28:50
                             Started mapping on |	May 06 14:28:50
                                    Finished on |	May 06 14:28:53
       Mapping speed, Million of reads per hour |	0.25

                          Number of input reads |	209
                      Average input read length |	69
                                    UNIQUE READS:
                   Uniquely mapped reads number |	87
                        Uniquely mapped reads % |	41.63%
                          Average mapped length |	69.29
                       Number of splices: Total |	3
            Number of splices: Annotated (sjdb) |	3
                       Number of splices: GT/AG |	3
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	0.96%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.03%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	51
             % of reads mapped to multiple loci |	24.40%
        Number of reads mapped to too many loci |	4
             % of reads mapped to too many loci |	1.91%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	32.06%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	71	71	71
N_multimapping	51	51	51
N_noFeature	60	75	71
N_ambiguous	3	2	0
UnstrandedReadsAssigned:24 PositiveStrandReadsAssigned:10 NegativeStrandReadsAssigned:16
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13210234 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13210234-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 209 reads, 31 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 310 rounds

 180870 SRR13210234.ke.tsv
  58302 SRR13210234.se.tsv
 239172 total
==> SRR13210234.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13210234.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210234 completed mapping pipeline successfully
