Starting /dee2/code/volunteer_pipeline.sh SRR13210466
    current disk space = 1769078294136
    free memory = 351377876 
SRR13210466 SRAfilesize
a8326189e0de9534d92c16a408cfca81  SRR13210466.sra
SRR13210466.sra file validated
SRR13210466 is single end
SRR13210466 is conventional basespace
SRR13210466 read1 length is 35-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227
Sequences flagged as poor quality	0
Sequence length	35-71
%GC	17
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.894273127753305	32.0	32.0	32.0	14.0	32.0
2	30.73127753303965	32.0	32.0	32.0	32.0	32.0
3	31.105726872246695	32.0	32.0	32.0	32.0	32.0
4	30.47136563876652	32.0	32.0	32.0	21.0	32.0
5	31.105726872246695	32.0	32.0	32.0	32.0	32.0
6	34.22466960352423	36.0	36.0	36.0	32.0	36.0
7	33.77092511013216	36.0	36.0	36.0	21.0	36.0
8	33.797356828193834	36.0	36.0	36.0	21.0	36.0
9	33.841409691629956	36.0	36.0	36.0	32.0	36.0
10	34.14537444933921	36.0	36.0	36.0	32.0	36.0
11	34.36563876651982	36.0	36.0	36.0	32.0	36.0
12	34.096916299559474	36.0	36.0	36.0	21.0	36.0
13	34.38766519823788	36.0	36.0	36.0	32.0	36.0
14	33.83700440528634	36.0	36.0	36.0	21.0	36.0
15	33.77092511013216	36.0	36.0	36.0	21.0	36.0
16	34.51101321585903	36.0	36.0	36.0	32.0	36.0
17	33.70484581497797	36.0	36.0	36.0	21.0	36.0
18	33.65638766519824	36.0	36.0	36.0	21.0	36.0
19	33.91629955947136	36.0	36.0	36.0	21.0	36.0
20	33.56828193832599	36.0	36.0	36.0	21.0	36.0
21	34.19383259911894	36.0	36.0	36.0	32.0	36.0
22	33.48898678414097	36.0	36.0	36.0	21.0	36.0
23	33.969162995594715	36.0	36.0	36.0	27.0	36.0
24	33.65638766519824	36.0	36.0	36.0	21.0	36.0
25	33.50660792951542	36.0	36.0	36.0	21.0	36.0
26	33.25550660792952	36.0	36.0	36.0	21.0	36.0
27	34.0352422907489	36.0	36.0	36.0	32.0	36.0
28	34.0	36.0	36.0	36.0	32.0	36.0
29	33.198237885462554	36.0	36.0	36.0	21.0	36.0
30	33.669603524229075	36.0	36.0	36.0	21.0	36.0
31	33.070484581497794	36.0	36.0	36.0	21.0	36.0
32	32.51541850220264	36.0	36.0	36.0	14.0	36.0
33	33.18942731277533	36.0	36.0	36.0	21.0	36.0
34	32.59471365638767	36.0	36.0	36.0	14.0	36.0
35	32.92070484581498	36.0	36.0	36.0	21.0	36.0
36	33.05777777777778	36.0	36.0	36.0	21.0	36.0
37	32.08888888888889	36.0	32.0	36.0	14.0	36.0
38	32.364444444444445	36.0	36.0	36.0	14.0	36.0
39	32.471111111111114	36.0	36.0	36.0	14.0	36.0
40	32.49333333333333	36.0	32.0	36.0	14.0	36.0
41	31.92	36.0	32.0	36.0	14.0	36.0
42	31.844444444444445	36.0	32.0	36.0	14.0	36.0
43	32.22666666666667	36.0	32.0	36.0	14.0	36.0
44	32.77777777777778	36.0	36.0	36.0	21.0	36.0
45	32.14666666666667	36.0	36.0	36.0	14.0	36.0
46	32.08444444444444	36.0	32.0	36.0	14.0	36.0
47	31.657777777777778	36.0	32.0	36.0	14.0	36.0
48	31.56888888888889	36.0	32.0	36.0	14.0	36.0
49	32.03111111111111	36.0	32.0	36.0	14.0	36.0
50	30.533333333333335	36.0	21.0	36.0	14.0	36.0
51	31.69333333333333	36.0	32.0	36.0	14.0	36.0
52	31.924444444444443	36.0	32.0	36.0	14.0	36.0
53	31.697777777777777	36.0	32.0	36.0	14.0	36.0
54	31.40888888888889	36.0	32.0	36.0	14.0	36.0
55	31.782222222222224	36.0	32.0	36.0	14.0	36.0
56	31.355555555555554	36.0	32.0	36.0	14.0	36.0
57	32.03111111111111	36.0	32.0	36.0	14.0	36.0
58	31.16888888888889	36.0	32.0	36.0	14.0	36.0
59	31.12	36.0	32.0	36.0	14.0	36.0
60	30.813333333333333	36.0	32.0	36.0	14.0	36.0
61	31.035555555555554	36.0	32.0	36.0	14.0	36.0
62	30.844444444444445	36.0	32.0	36.0	14.0	36.0
63	31.102222222222224	36.0	32.0	36.0	14.0	36.0
64	30.293333333333333	36.0	21.0	36.0	14.0	36.0
65	30.555555555555557	36.0	27.0	36.0	14.0	36.0
66	30.444444444444443	36.0	21.0	36.0	14.0	36.0
67	30.32	36.0	21.0	36.0	14.0	36.0
68	30.79111111111111	36.0	32.0	36.0	14.0	36.0
69	29.13963963963964	36.0	14.0	36.0	14.0	36.0
70	30.16289592760181	36.0	21.0	36.0	14.0	36.0
71	29.878048780487806	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	4.0
24	7.0
25	8.0
26	8.0
27	9.0
28	9.0
29	7.0
30	7.0
31	17.0
32	10.0
33	26.0
34	37.0
35	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.396475770925107	53.30396475770925	6.607929515418502	9.691629955947137
2	7.048458149779736	53.74449339207048	27.75330396475771	11.45374449339207
3	11.894273127753303	66.07929515418502	12.77533039647577	9.251101321585903
4	3.0837004405286343	53.74449339207048	20.26431718061674	22.90748898678414
5	5.726872246696035	62.55506607929515	19.383259911894275	12.334801762114537
6	14.977973568281937	63.87665198237885	13.215859030837004	7.929515418502203
7	14.537444933920703	59.91189427312775	17.180616740088105	8.370044052863436
8	13.656387665198238	56.38766519823789	15.418502202643172	14.537444933920703
9	16.740088105726873	50.22026431718062	18.06167400881057	14.977973568281937
10	6.607929515418502	58.590308370044056	19.823788546255507	14.977973568281937
11	13.215859030837004	58.590308370044056	16.299559471365637	11.894273127753303
12	12.334801762114537	55.947136563876654	25.11013215859031	6.607929515418502
13	13.656387665198238	57.70925110132159	22.026431718061673	6.607929515418502
14	12.334801762114537	56.38766519823789	21.58590308370044	9.691629955947137
15	11.45374449339207	55.06607929515418	25.11013215859031	8.370044052863436
16	9.691629955947137	59.03083700440529	21.58590308370044	9.691629955947137
17	6.167400881057269	55.50660792951542	24.669603524229075	13.656387665198238
18	7.929515418502203	55.50660792951542	28.193832599118945	8.370044052863436
19	9.251101321585903	53.74449339207048	28.634361233480178	8.370044052863436
20	7.048458149779736	57.70925110132159	29.515418502202646	5.726872246696035
21	8.370044052863436	54.18502202643172	29.074889867841406	8.370044052863436
22	10.572687224669604	54.62555066079295	31.277533039647576	3.524229074889868
23	11.45374449339207	54.18502202643172	30.837004405286343	3.524229074889868
24	8.81057268722467	55.06607929515418	28.193832599118945	7.929515418502203
25	6.637168141592921	54.86725663716814	28.31858407079646	10.176991150442479
26	6.637168141592921	57.07964601769911	30.53097345132743	5.752212389380531
27	10.176991150442479	55.309734513274336	30.53097345132743	3.982300884955752
28	7.079646017699115	53.53982300884957	30.973451327433626	8.4070796460177
29	7.52212389380531	55.309734513274336	30.53097345132743	6.637168141592921
30	5.752212389380531	55.309734513274336	33.6283185840708	5.3097345132743365
31	7.52212389380531	53.09734513274337	29.646017699115045	9.734513274336283
32	6.666666666666667	52.0	31.11111111111111	10.222222222222223
33	8.0	53.333333333333336	29.333333333333332	9.333333333333334
34	6.222222222222222	56.00000000000001	30.22222222222222	7.555555555555555
35	5.777777777777778	56.888888888888886	28.888888888888886	8.444444444444445
36	7.555555555555555	54.666666666666664	30.666666666666664	7.111111111111111
37	7.555555555555555	56.00000000000001	30.22222222222222	6.222222222222222
38	10.222222222222223	54.22222222222223	28.000000000000004	7.555555555555555
39	5.777777777777778	55.55555555555556	29.333333333333332	9.333333333333334
40	7.555555555555555	57.77777777777777	28.888888888888886	5.777777777777778
41	7.111111111111111	55.111111111111114	29.333333333333332	8.444444444444445
42	9.777777777777779	52.44444444444445	28.888888888888886	8.88888888888889
43	7.111111111111111	56.00000000000001	28.000000000000004	8.88888888888889
44	8.88888888888889	55.111111111111114	28.000000000000004	8.0
45	7.111111111111111	56.00000000000001	27.111111111111114	9.777777777777779
46	7.111111111111111	56.44444444444444	27.555555555555557	8.88888888888889
47	8.0	56.44444444444444	27.555555555555557	8.0
48	8.444444444444445	58.666666666666664	26.222222222222225	6.666666666666667
49	8.444444444444445	55.111111111111114	26.222222222222225	10.222222222222223
50	9.333333333333334	57.333333333333336	24.88888888888889	8.444444444444445
51	4.444444444444445	60.0	27.555555555555557	8.0
52	7.111111111111111	58.22222222222222	27.111111111111114	7.555555555555555
53	10.222222222222223	55.55555555555556	26.666666666666668	7.555555555555555
54	8.444444444444445	60.0	21.777777777777775	9.777777777777779
55	7.555555555555555	59.55555555555555	25.77777777777778	7.111111111111111
56	8.0	59.111111111111114	26.666666666666668	6.222222222222222
57	8.88888888888889	57.333333333333336	24.88888888888889	8.88888888888889
58	7.111111111111111	58.22222222222222	28.000000000000004	6.666666666666667
59	6.222222222222222	60.44444444444444	24.444444444444443	8.88888888888889
60	7.555555555555555	58.22222222222222	23.555555555555554	10.666666666666668
61	7.111111111111111	58.22222222222222	26.666666666666668	8.0
62	8.444444444444445	58.22222222222222	24.444444444444443	8.88888888888889
63	4.0	60.88888888888889	25.77777777777778	9.333333333333334
64	7.555555555555555	56.888888888888886	25.333333333333336	10.222222222222223
65	8.88888888888889	56.888888888888886	24.444444444444443	9.777777777777779
66	8.444444444444445	55.111111111111114	25.77777777777778	10.666666666666668
67	9.333333333333334	54.666666666666664	27.111111111111114	8.88888888888889
68	8.444444444444445	58.666666666666664	25.333333333333336	7.555555555555555
69	8.558558558558559	59.009009009009006	25.225225225225223	7.207207207207207
70	9.049773755656108	55.203619909502265	29.86425339366516	5.88235294117647
71	9.268292682926829	62.926829268292686	27.80487804878049	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	45.0
2	7.0
3	11.0
4	8.5
5	4.0
6	2.0
7	2.0
8	3.5
9	6.0
10	7.0
11	10.5
12	9.5
13	3.5
14	2.0
15	1.5
16	2.0
17	3.0
18	3.0
19	2.0
20	1.0
21	1.0
22	2.0
23	2.5
24	2.0
25	1.0
26	1.5
27	1.5
28	0.0
29	0.5
30	1.0
31	1.0
32	1.5
33	2.5
34	2.5
35	3.5
36	4.5
37	2.5
38	0.5
39	1.0
40	4.0
41	6.0
42	4.0
43	2.5
44	2.0
45	0.5
46	2.0
47	3.5
48	2.5
49	2.0
50	3.5
51	5.0
52	5.5
53	5.5
54	4.0
55	2.5
56	3.5
57	3.5
58	2.0
59	3.5
60	4.0
61	1.5
62	0.0
63	0.0
64	0.5
65	1.0
66	0.5
67	0.5
68	1.0
69	1.0
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.4405286343612335
26	0.4405286343612335
27	0.4405286343612335
28	0.4405286343612335
29	0.4405286343612335
30	0.4405286343612335
31	0.4405286343612335
32	0.881057268722467
33	0.881057268722467
34	0.881057268722467
35	0.881057268722467
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	2.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	3.0
69	1.0
70	16.0
71	205.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.70925110132159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.89312977099237	54.18502202643172
2	3.816793893129771	4.405286343612335
3	0.0	0.0
4	0.0	0.0
5	0.7633587786259541	2.2026431718061676
6	0.0	0.0
7	0.7633587786259541	3.0837004405286343
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.7633587786259541	36.12334801762114
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	36.12334801762114	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	3.0837004405286343	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	2.2026431718061676	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.881057268722467	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.881057268722467	No Hit
GTCTTTTACTCTGCACCACACTAAGAAAAAACCAAGTGCAATTTCTCACTCCCTAACAAAATGCCCGACTT	2	0.881057268722467	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.881057268722467	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.881057268722467	No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAATATCGGGG	1	0.4405286343612335	No Hit
GTATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	1	0.4405286343612335	No Hit
CTTCAGTCTCCATGATTGCCAGTAGAAAGCCAACAGACGGTGCTTCCTCATCAAATTGTGTGACTGATATT	1	0.4405286343612335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTATTTTTTT	1	0.4405286343612335	No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTAAGACCCCCCAAAATTAAGGGGGGTTTAAA	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTACCCCAGACAAAAATGGTTTAAAAAAAATAGGCCGCATTAAA	1	0.4405286343612335	No Hit
GATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
GGTAGATTCCATTCCATTCCAATCCCCTCCCTGCCATTCCCCTCGTGAAGATTCCATTATCTCCATTCAAG	1	0.4405286343612335	No Hit
GAACTGGTATGAGCAGGATTTCTGCAGGTTCTTCTTCCTGAAGCTGAGGCTCAGGGGTGTGCCTGTCTGTT	1	0.4405286343612335	No Hit
CAGTGGTGCTACAAGCCTCTGTGACTTTCCCTTTAACTCTGTAGCTGCCAGCGCAGCTGATTTCTCTCTTG	1	0.4405286343612335	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTATT	1	0.4405286343612335	No Hit
GAGCGAGACTCCATCCCAAAAAAAAAAAAAAAAAAAAAAATAACCTGGGGATAATAACAATACCCCCCCC	1	0.4405286343612335	No Hit
ATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCAGGTATTAGCTCTAGAATTACCACAGTTA	1	0.4405286343612335	No Hit
CTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAAT	1	0.4405286343612335	No Hit
CTAGCACATGCAGTTGGAAATGTTGAACTCTACTTACATGACTGGGGAGTTGATTTTGCCTGCTGGTGTT	1	0.4405286343612335	No Hit
ACGCAGAGTACATGGGTGTTGAGCTTGAACGCTTTCTTAAATGGTGGCTGCATTTAGGCCTACTATGGGTG	1	0.4405286343612335	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTT	1	0.4405286343612335	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
GTATAAACGAAAAATAAAAATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAA	1	0.4405286343612335	No Hit
ATCAACGCAGAGTACATGGGTGTGGTATAAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
TAGTACTTTTTTTTTTTTTTTTTTTTTTATATGGGTTAGAAAACCAAAAAAAAATGGTAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
AAATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAATAGGGAGAATAAAGAAAAAA	1	0.4405286343612335	No Hit
GCCTTGCTGATAGCGATCATTTACAAGATTCGCTAAAGAAATTGCCTCTCCTTATTCCTGAGCCTGCAGTT	1	0.4405286343612335	No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAGCATGG	1	0.4405286343612335	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAAAAACATATAAAAACATAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
CAGTCAGCATGTCCAGCCTGGGGTCTGGGTGTAGGGTTATCCCTTCTCCCTGTGCCTTCCCATCTCGTCCA	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTAAAAAAACCCAATAAAAAGAAGGTTAAA	1	0.4405286343612335	No Hit
GTGTATGTACTTATGGGGTACATGAGATATTTTGATACAGACATACAATGCATAATAATCACATCAGGGTA	1	0.4405286343612335	No Hit
GGAAAACACTCTGAGATACCAGTACAATGTTAAACATTTACTCACCAAAATCCCATAAATATCTCCTGGAT	1	0.4405286343612335	No Hit
GAGTACATGGGGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
AAAAGAAAAAAAAAAATACTCTGCGTTTATANNNN	1	0.4405286343612335	No Hit
ATAAATAGTACAAAAAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAACAAAAAAAAATAA	1	0.4405286343612335	No Hit
GCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGTGCCCCGCGGGACACTCAGCTAA	1	0.4405286343612335	No Hit
ATAGCTGACAGTATGCTTGATTGAAAGTTTGGGTCTTCTTGCCAGGCAATATGCCATGAAGTTAGATAACA	1	0.4405286343612335	No Hit
GCGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
AGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATG	1	0.4405286343612335	No Hit
TCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGG	1	0.4405286343612335	No Hit
CTATTAATGATTGATAAGCACCAGTGAAGAAGTTCTAACTTTTAGCATGCTGCACAGAAACTGGTATAACA	1	0.4405286343612335	No Hit
TTGATATCTCCTTGTGGAAATTTGAGACCAGCAAGTACTATGTGACTATCATTGATGCCCCAGGACACAGA	1	0.4405286343612335	No Hit
CCACTGCTTCCCATGTACTCTGAGTTGATACCACTGCTTATCCATTTTAAAATGTGTTAAAACCACAGCAG	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAA	1	0.4405286343612335	No Hit
AACCTGCCGGGCATGGATTCGATTCCTACTTTATGAAGAGCACTCGGCGCACGGTGAGGCCCTGCCCAGG	1	0.4405286343612335	No Hit
CGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGG	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAGGGGTTAAACCCAATAAAATTTAAAAAAAAAA	1	0.4405286343612335	No Hit
ATTCCGGAGAGGGAGCCTGAGAAATGGCAAACGCATCCAAGGAACGCAGCAGGCCAGCAAATTACCCAAT	1	0.4405286343612335	No Hit
GGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGA	1	0.4405286343612335	No Hit
ACGCAGAGTACATGGGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTGTTTTT	1	0.4405286343612335	No Hit
GGCTTATGCAGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGG	1	0.4405286343612335	No Hit
GTAATATGATGCCTCCAGCTTTGTTCTTTTTGCTTAGATTTGCTTTGGCTATTTGAGCTCTTTTTTGGTTT	1	0.4405286343612335	No Hit
GTATCAACGGAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTT	1	0.4405286343612335	No Hit
ATATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
ATGCAGAAGGGATGGGTGATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGACTTGTTGGGT	1	0.4405286343612335	No Hit
TCTGTAAGCAGTTTGTATGTTTAGTTGGGGTAATGAAGTATTTCAGTTTTGTTAATAGATGACCTGTTTTT	1	0.4405286343612335	No Hit
GCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGCCGTCAACGAGGTCCGCTGC	1	0.4405286343612335	No Hit
AAACATGACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
CTCATACGGCATCCGATGAGCTAGACTTATTCTTCTGGGTGAGTACTGCTCAACCCTGGCTGCAGGGTACA	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAACAAT	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAA	1	0.4405286343612335	No Hit
TATAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAA	1	0.4405286343612335	No Hit
GCCTCATGTTCACCTCCCTAGGAAAAGGGCCAAGCCAAACCCTGGAGAAGTTATTCCCAAATGAGGGGAG	1	0.4405286343612335	No Hit
GGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGG	1	0.4405286343612335	No Hit
GTAAGGGTGACTGATACTGCAGGCATATTTTTTCTTTATATTTCAAGAACACTGTGTACACAAAGTGCTTG	1	0.4405286343612335	No Hit
AAGTAATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAACAA	1	0.4405286343612335	No Hit
GGTATCAACGCAGAGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAAAAAAAAAAAAATTAAAA	1	0.4405286343612335	No Hit
AGGAAAAACAAGAAACAAAACAGAATAAAAAGGAAGCGGAAGTAAAAAAGCAAGAGAAAGAAAAAAAAAAA	1	0.4405286343612335	No Hit
CAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGAAAAACACCAAAATAAAAAGATT	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTGGAAAAAAAGTAATAGTGTG	1	0.4405286343612335	No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCGCTGCTTCGCAGGCGGCAAAG	1	0.4405286343612335	No Hit
TAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
AAATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
TCCCTGGAGTTCTTGCAAGTCGGCCAGGATGTCTCAGGCTGAGTTTGAGAAAGCTGCAGAGGAGGTTAGG	1	0.4405286343612335	No Hit
ATTCAGTGTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCTCCACTTTTTCA	1	0.4405286343612335	No Hit
GTTGCGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	1	0.4405286343612335	No Hit
GTACATGGGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
ACCTCATACTTCTCTGTTCACTCTCATTGTGTAATCTTGCTTCATATTTCATTGAGAAAATAAAAGCAGT	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAATA	1	0.4405286343612335	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTATTATAAGAGCAACCCCCACCCAAAAAGAATT	1	0.4405286343612335	No Hit
CAAAACTGGCTGTGCATAATAATTGCTTATTTAACTTTTTAGGTGTTACCACCCACAGAGAGTCAAAACCA	1	0.4405286343612335	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAAAATAAAAAAAAAAAAAATAAAAT	1	0.4405286343612335	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGT	1	0.4405286343612335	No Hit
GGTAAAAAAATCCATAATAAACGACAACATTTTGAAGAAGACAAAAGTTGGGCAGTTGACACTACCCAACA	1	0.4405286343612335	No Hit
CCTCACAGCCTCCTTCAGAGGAAACCACATGTTGACAAAGAGACCTTTTCCCCACTCCGGGAGGGACTGCA	1	0.4405286343612335	No Hit
GTACATGGGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAA	1	0.4405286343612335	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTTA	1	0.4405286343612335	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTACTTTTTTTCTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
CGTCAGGAAGTCTCAGGGGCCGATTATCTTGGGAAGTGACTTTCAGAGGAATCCCCATGTCGTCGAAACGG	1	0.4405286343612335	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAATTAAAGGGGGGTTTAAACATGGGGATTAAAAAAAAAACCCAACCCACGAA	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAACAAAAAAAAAAACAA	1	0.4405286343612335	No Hit
TCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATTGTCGGAACTACGACG	1	0.4405286343612335	No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTT	1	0.4405286343612335	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTG	1	0.4405286343612335	No Hit
CTTCTAAGGAATGACTTGGTCAGCTCTGTGCTTTCTTGCCTTCAAGAGTGTGTGCTGGGCGCTGGCACTG	1	0.4405286343612335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTATTTTTTTTAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.4405286343612335	No Hit
CCCATTCCCCCTGGACATTAAAGCCAAAGACTCAGATGACTTTGACCGATAATACCGCACCCCTACAC	1	0.4405286343612335	No Hit
GGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGCCGTGCGTACTTAGACATGCATGT	1	0.4405286343612335	No Hit
GTCCCAGCTACTCGGGAGCTAAGTCAGGAGAATTGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAGCCAAG	1	0.4405286343612335	No Hit
AAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAACAAAAAAAAA	1	0.4405286343612335	No Hit
CCTCCAGGGCGTAGTAGGAAGACGGCATGGTGTACTGGAAAGGGCATGGGAAGGGAGAGCTCCCCAAACC	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACCCAATTTAAGAAGGGTTGAAAACACAAGA	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGTATTTTTTTTTTTATTTAGTTTTTTTTGTAGG	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGATAGACCAACCAATGAGGAG	1	0.4405286343612335	No Hit
GTACATGGGAAAAAAAAAAAAAACNNNNNNNNNNN	1	0.4405286343612335	No Hit
GCTTTAATTTATTAATGCAAACAGTACCTAACAAAACCACCGGTCCTAAACTACCACACCTGCAGTAAAAA	1	0.4405286343612335	No Hit
GAGTGAGACTCTGTCTCAAAAAAAAAAGTTTCAATGTTTACTCCTAGAGAAGCCAAAAATCCAGATTTGTA	1	0.4405286343612335	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTTTTA	1	0.4405286343612335	No Hit
GTTGATTTAAGTCTCGATATGGGCTTCCTTTTTTCTCTCCTCCCTCCCATCTAATAACCCTTATCCTGAAT	1	0.4405286343612335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAATAAGAGGAATAAGATTA	1	0.4405286343612335	No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG	1	0.4405286343612335	No Hit
GTCCTGGGTTTTCCTACTGAAGAGCTTGTGGGCCTCTGTTTTCTGATACTGTTCCAGTTCCGTCATGTAA	1	0.4405286343612335	No Hit
GTATTAGGGCGGCCCTCAGCTGTGGTGTGGCAGCCTCTGGGCAGCACAGAATCCAATCCTAAAGGGCCAC	1	0.4405286343612335	No Hit
GTCTAGCCTAGTGCCTGACATTCACTTCAGCTCATCTCTTGCTAAATGCCATTGGTGCACATTGCCCCC	1	0.4405286343612335	No Hit
CAGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCGGGAGCGGTGGCAGGCTCCTGTAATCCCAG	1	0.4405286343612335	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.4405286343612335	No Hit
GGGTAGAAACTGCAAGGGGTGGTAAAGATTGAACAGACTAGGAAATAGGTTATTCTTTTTTATTAATTTTT	1	0.4405286343612335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 4 READS because READLEN < 1
Read 4 spots for SRR13210466.sra
Written 4 spots for SRR13210466.sra
Rejected 39 READS because READLEN < 1
Read 39 spots for SRR13210466.sra
Written 39 spots for SRR13210466.sra
SRR ids: ['SRR13210466.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_pw8nn_cg
SRR13210466.sra spots: 227
blocks: [[1, 4], [5, 8], [9, 12], [13, 16], [17, 20], [21, 24], [25, 28], [29, 32], [33, 36], [37, 40], [41, 44], [45, 48], [49, 52], [53, 56], [57, 60], [61, 64], [65, 68], [69, 72], [73, 76], [77, 80], [81, 84], [85, 88], [89, 92], [93, 96], [97, 100], [101, 104], [105, 108], [109, 112], [113, 116], [117, 120], [121, 124], [125, 128], [129, 132], [133, 136], [137, 140], [141, 144], [145, 148], [149, 152], [153, 156], [157, 160], [161, 164], [165, 168], [169, 172], [173, 176], [177, 180], [181, 184], [185, 188], [189, 227]]
SRR13210466 file size 1
SRR13210466 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13210466 SRR13210466_1.fastq
Input file:	SRR13210466_1.fastq
trimmed:	SRR13210466-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 12:38:10 2022 >> started

Fri May  6 12:38:10 2022 >> done (0.005s)
227 reads processed; of these:
  0 ( 0.00%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
227 (100.00%) reads available; of these:
  2 ( 0.88%) trimmed reads available after processing
225 (99.12%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 24	  1	  0.44%
 25	  0	  0.00%
 26	  0	  0.00%
 27	  0	  0.00%
 28	  0	  0.00%
 29	  0	  0.00%
 30	  0	  0.00%
 31	  1	  0.44%
 32	  0	  0.00%
 33	  0	  0.00%
 34	  0	  0.00%
 35	  0	  0.00%
 36	  0	  0.00%
 37	  0	  0.00%
 38	  0	  0.00%
 39	  0	  0.00%
 40	  0	  0.00%
 41	  0	  0.00%
 42	  0	  0.00%
 43	  0	  0.00%
 44	  0	  0.00%
 45	  0	  0.00%
 46	  0	  0.00%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  3	  1.32%
 69	  1	  0.44%
 70	 16	  7.05%
 71	205	 90.31%
227 reads passed initial QC
                                 Started job on |	May 06 12:38:28
                             Started mapping on |	May 06 12:38:29
                                    Finished on |	May 06 12:38:32
       Mapping speed, Million of reads per hour |	0.27

                          Number of input reads |	227
                      Average input read length |	62
                                    UNIQUE READS:
                   Uniquely mapped reads number |	65
                        Uniquely mapped reads % |	28.63%
                          Average mapped length |	58.18
                       Number of splices: Total |	2
            Number of splices: Annotated (sjdb) |	2
                       Number of splices: GT/AG |	2
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	0.95%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.03%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	41
             % of reads mapped to multiple loci |	18.06%
        Number of reads mapped to too many loci |	6
             % of reads mapped to too many loci |	2.64%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.69%
                     % of reads unmapped: other |	40.97%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	121	121	121
N_multimapping	41	41	41
N_noFeature	43	48	59
N_ambiguous	1	0	0
UnstrandedReadsAssigned:21 PositiveStrandReadsAssigned:17 NegativeStrandReadsAssigned:6
Dataset is classified unstranded
MeadianReadLen=63 20thPercentileLength=63 echo kmer=59
SRR13210466 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13210466-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 227 reads, 148 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 472 rounds

 180870 SRR13210466.ke.tsv
  58302 SRR13210466.se.tsv
 239172 total
==> SRR13210466.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13210466.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210466 completed mapping pipeline successfully
