Starting /dee2/code/volunteer_pipeline.sh SRR13209945
    current disk space = 2117016976
    free memory = 132409936 
SRR13209945 SRAfilesize
6d8fbbeec7bb3c7b893069dbb5e5157f  SRR13209945.sra
SRR13209945.sra file validated
SRR13209945 is single end
SRR13209945 is conventional basespace
SRR13209945 read1 length is 35-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13209945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35-71
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5255	32.0	32.0	32.0	32.0	32.0
2	31.707	32.0	32.0	32.0	32.0	32.0
3	31.74225	32.0	32.0	32.0	32.0	32.0
4	31.83225	32.0	32.0	32.0	32.0	32.0
5	31.83275	32.0	32.0	32.0	32.0	32.0
6	35.4375	36.0	36.0	36.0	36.0	36.0
7	35.592	36.0	36.0	36.0	36.0	36.0
8	35.4	36.0	36.0	36.0	36.0	36.0
9	35.547	36.0	36.0	36.0	36.0	36.0
10	35.497	36.0	36.0	36.0	36.0	36.0
11	35.45925	36.0	36.0	36.0	36.0	36.0
12	35.47075	36.0	36.0	36.0	36.0	36.0
13	35.41825	36.0	36.0	36.0	36.0	36.0
14	35.301	36.0	36.0	36.0	36.0	36.0
15	35.0905	36.0	36.0	36.0	36.0	36.0
16	35.29025	36.0	36.0	36.0	36.0	36.0
17	35.179	36.0	36.0	36.0	36.0	36.0
18	35.1795	36.0	36.0	36.0	36.0	36.0
19	35.3875	36.0	36.0	36.0	36.0	36.0
20	35.463	36.0	36.0	36.0	36.0	36.0
21	35.58125	36.0	36.0	36.0	36.0	36.0
22	35.5425	36.0	36.0	36.0	36.0	36.0
23	35.54075	36.0	36.0	36.0	36.0	36.0
24	35.518	36.0	36.0	36.0	36.0	36.0
25	35.399	36.0	36.0	36.0	36.0	36.0
26	35.41	36.0	36.0	36.0	36.0	36.0
27	35.232	36.0	36.0	36.0	36.0	36.0
28	35.06825	36.0	36.0	36.0	36.0	36.0
29	34.768	36.0	36.0	36.0	32.0	36.0
30	34.86425	36.0	36.0	36.0	32.0	36.0
31	35.167	36.0	36.0	36.0	36.0	36.0
32	35.35625	36.0	36.0	36.0	36.0	36.0
33	35.3465	36.0	36.0	36.0	36.0	36.0
34	35.32675	36.0	36.0	36.0	36.0	36.0
35	35.3425	36.0	36.0	36.0	36.0	36.0
36	35.31481481481482	36.0	36.0	36.0	36.0	36.0
37	35.02202753441802	36.0	36.0	36.0	36.0	36.0
38	34.80946419629444	36.0	36.0	36.0	36.0	36.0
39	34.13899323816679	36.0	36.0	36.0	32.0	36.0
40	33.544224505136555	36.0	36.0	36.0	27.0	36.0
41	33.17619047619048	36.0	36.0	36.0	14.0	36.0
42	32.80375939849624	36.0	36.0	36.0	14.0	36.0
43	33.4358395989975	36.0	36.0	36.0	27.0	36.0
44	34.58886939082477	36.0	36.0	36.0	32.0	36.0
45	35.089814350225794	36.0	36.0	36.0	36.0	36.0
46	35.24993726474278	36.0	36.0	36.0	36.0	36.0
47	35.18709191361125	36.0	36.0	36.0	36.0	36.0
48	35.07209243908566	36.0	36.0	36.0	36.0	36.0
49	34.730082935410906	36.0	36.0	36.0	36.0	36.0
50	34.44617706237425	36.0	36.0	36.0	32.0	36.0
51	34.723955712128834	36.0	36.0	36.0	36.0	36.0
52	35.02896725440806	36.0	36.0	36.0	36.0	36.0
53	35.03706505295008	36.0	36.0	36.0	36.0	36.0
54	35.14941948510853	36.0	36.0	36.0	36.0	36.0
55	34.99823232323232	36.0	36.0	36.0	36.0	36.0
56	35.05509224159717	36.0	36.0	36.0	36.0	36.0
57	34.642315470171894	36.0	36.0	36.0	36.0	36.0
58	34.25859453993933	36.0	36.0	36.0	32.0	36.0
59	34.00278058645096	36.0	36.0	36.0	32.0	36.0
60	33.85819009100101	36.0	36.0	36.0	32.0	36.0
61	33.6049632818435	36.0	36.0	36.0	27.0	36.0
62	33.323447401774395	36.0	36.0	36.0	21.0	36.0
63	33.09155465381689	36.0	36.0	36.0	14.0	36.0
64	34.17812737883786	36.0	36.0	36.0	32.0	36.0
65	34.95810055865922	36.0	36.0	36.0	36.0	36.0
66	34.84277368554737	36.0	36.0	36.0	36.0	36.0
67	34.942522889114954	36.0	36.0	36.0	36.0	36.0
68	35.02171691364333	36.0	36.0	36.0	36.0	36.0
69	34.95352480417755	36.0	36.0	36.0	36.0	36.0
70	34.88284753363229	36.0	36.0	36.0	36.0	36.0
71	33.79601990049751	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	4.0
23	1.0
24	3.0
25	12.0
26	15.0
27	21.0
28	40.0
29	55.0
30	70.0
31	90.0
32	158.0
33	274.0
34	660.0
35	2595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.800000000000004	19.35	12.3	22.55
2	16.375	20.025000000000002	40.2	23.400000000000002
3	19.575	27.125	28.325	24.975
4	12.2	16.825000000000003	36.449999999999996	34.525
5	15.024999999999999	39.2	29.275000000000002	16.5
6	29.022556390977446	37.669172932330824	17.593984962406015	15.714285714285714
7	31.4	31.0	18.775	18.825
8	31.225	30.099999999999998	18.325	20.349999999999998
9	31.275	15.024999999999999	17.5	36.199999999999996
10	19.5	25.35	25.95	29.2
11	37.425000000000004	23.35	19.975	19.25
12	27.025	24.7	27.125	21.15
13	28.525	20.825	28.025	22.625
14	23.799999999999997	23.7	23.95	28.549999999999997
15	24.825	26.85	24.2	24.125
16	23.05	30.375000000000004	22.325	24.25
17	24.5	26.5	23.125	25.874999999999996
18	23.025000000000002	25.25	26.8	24.925
19	27.175	26.875	26.55	19.400000000000002
20	27.775	22.25	26.8	23.175
21	31.125000000000004	21.75	25.2	21.925
22	29.049999999999997	22.8	24.925	23.225
23	26.93173293323331	22.605651412853213	25.98149537384346	24.48112028007002
24	25.70642660665166	24.381095273818453	23.93098274568642	25.98149537384346
25	25.56278139069535	24.337168584292147	24.437218609304654	25.662831415707853
26	25.78789394697349	24.112056028014006	26.738369184592298	23.36168084042021
27	26.263131565782892	23.56178089044522	25.53776888444222	24.637318659329665
28	25.03751875937969	25.46273136568284	25.437718859429715	24.062031015507753
29	24.662331165582792	24.537268634317158	26.638319159579787	24.16208104052026
30	25.937968984492244	23.936968484242122	27.03851925962982	23.08654327163582
31	24.062031015507753	26.538269134567283	28.46423211605803	20.935467733866933
32	23.81190595297649	23.461730865432717	27.388694347173587	25.337668834417208
33	24.44333249937453	23.317488116087066	25.31898924193145	26.920190142606952
34	24.86865148861646	24.893670252689517	27.77082812109082	22.466850137603203
35	24.54340755566675	24.69352014010508	27.695771828871653	23.067300475356518
36	25.150150150150154	24.024024024024023	27.87787787787788	22.94794794794795
37	26.23279098873592	25.381727158948685	24.831038798498124	23.554443053817273
38	26.514772158237353	23.410115172759138	26.81522283425138	23.25988983475213
39	25.494615577260205	25.64487853744052	26.321061858251944	22.539444027047335
40	26.384364820846905	25.98346279128038	26.259082936607363	21.373089451265347
41	24.592629731762347	25.69566307345199	26.748558535973928	22.963148658811733
42	26.68004012036108	27.05616850551655	27.05616850551655	19.207622868605817
43	26.021559288042116	23.89069942341439	27.325144146402607	22.762597142140887
44	25.745800952619703	25.495111556781147	26.096766106793684	22.662321383805466
45	25.13798294029102	28.073256397390868	25.94079277471149	20.84796788760662
46	24.391468005018822	24.592220828105397	27.50313676286073	23.513174404015054
47	23.380210949271724	24.937217478653942	26.92114515318935	24.761426418884984
48	23.536799799045465	27.304697312233106	26.249686008540568	22.90881688018086
49	26.46393566222669	24.026137220407136	25.30786629806484	24.202060819301334
50	26.609657947686117	25.352112676056336	26.18209255533199	21.856136820925553
51	22.471061902365374	27.252138902868644	22.395571212883745	27.881227981882233
52	22.443324937027707	27.43073047858942	25.239294710327453	24.886649874055415
53	25.03782148260212	24.230963187090268	25.1386787695411	25.592536560766515
54	23.902069661786975	25.037859666834933	28.369510348308935	22.690560323069157
55	22.146464646464647	24.924242424242422	29.11616161616162	23.813131313131315
56	23.553196866312863	26.990144048521607	25.650745514278494	23.805913570887036
57	23.179979777553083	24.418604651162788	24.3680485338726	28.033367037411526
58	24.064711830131447	25.075834175935285	25.73306370070779	25.12639029322548
59	24.87360970677452	25.480283114256824	25.45500505561173	24.191102123356924
60	25.0	26.466127401415573	24.595551061678464	23.938321536905967
61	23.778171689035197	28.209673335021524	24.233983286908078	23.778171689035197
62	24.157160963244614	26.89480354879594	23.574144486692013	25.373891001267427
63	23.231042353537916	26.02079634795841	28.075069743849863	22.67309155465382
64	22.050241055569654	26.896726719106827	27.607206292819082	23.44582593250444
65	23.768410360589133	25.26663280853225	26.91721686135094	24.04773996952768
66	23.469646939293877	26.16205232410465	27.27965455930912	23.088646177292354
67	23.575788402848424	26.14445574771109	26.14445574771109	24.135300101729403
68	22.253449156872765	26.341338783852837	27.899846704138987	23.50536535513541
69	24.36031331592689	25.509138381201048	26.94516971279373	23.18537859007833
70	24.075112107623315	27.63452914798206	28.53139013452915	19.75896860986547
71	34.21354764638347	38.882510524301566	26.903941829314963	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	8.0
2	4.0
3	8.0
4	7.5
5	5.0
6	2.0
7	1.0
8	2.0
9	14.0
10	25.0
11	23.5
12	17.0
13	8.5
14	5.0
15	5.5
16	3.5
17	1.0
18	3.0
19	4.0
20	5.5
21	8.0
22	7.0
23	7.5
24	10.0
25	11.5
26	12.5
27	13.5
28	16.0
29	19.5
30	21.0
31	20.5
32	27.0
33	34.5
34	37.0
35	38.0
36	37.5
37	38.5
38	39.5
39	41.0
40	49.5
41	66.5
42	89.0
43	119.5
44	147.5
45	168.0
46	258.5
47	372.0
48	422.5
49	408.0
50	376.5
51	399.0
52	431.5
53	413.0
54	289.0
55	187.0
56	157.0
57	117.0
58	91.5
59	92.0
60	88.5
61	56.5
62	44.5
63	50.5
64	40.0
65	29.5
66	27.0
67	20.5
68	16.0
69	13.5
70	7.5
71	5.0
72	6.0
73	10.0
74	12.5
75	11.0
76	7.5
77	4.0
78	3.5
79	3.5
80	2.0
81	0.5
82	1.0
83	2.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.025
24	0.025
25	0.05
26	0.05
27	0.05
28	0.05
29	0.05
30	0.05
31	0.05
32	0.05
33	0.075
34	0.075
35	0.075
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.02506265664160401
42	0.05012531328320802
43	0.02506265664160401
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	4.0
36	1.0
37	1.0
38	1.0
39	2.0
40	1.0
41	0.0
42	0.0
43	1.0
44	3.0
45	1.0
46	3.0
47	1.0
48	2.0
49	3.0
50	2.0
51	4.0
52	4.0
53	4.0
54	2.0
55	3.0
56	1.0
57	0.0
58	0.0
59	0.0
60	7.0
61	4.0
62	2.0
63	2.0
64	3.0
65	1.0
66	5.0
67	18.0
68	84.0
69	262.0
70	955.0
71	2613.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.00523560209425	68.0
2	5.7591623036649215	8.799999999999999
3	1.963350785340314	4.5
4	1.0798429319371727	3.3000000000000003
5	0.5890052356020943	2.25
6	0.3926701570680628	1.7999999999999998
7	0.16361256544502617	0.8750000000000001
8	0.1963350785340314	1.2
9	0.0981675392670157	0.675
>10	0.7526178010471204	8.6
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAA	34	0.8500000000000001	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTT	23	0.575	No Hit
GTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCT	23	0.575	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCC	19	0.475	No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGG	17	0.42500000000000004	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.42500000000000004	No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	15	0.375	No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAAATTGATACCGTAACTTCGGGAGAAGGTA	14	0.35000000000000003	No Hit
ATTGACGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCAGG	14	0.35000000000000003	No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTTTTTGCA	14	0.35000000000000003	No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAA	14	0.35000000000000003	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.35000000000000003	No Hit
GTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTTTTTGCAACCCCTTTGGGCTCCCTCTGTACGAGT	13	0.325	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTA	13	0.325	No Hit
ACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCG	12	0.3	No Hit
CTGTACGAGTTCACCTACTTGGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCT	12	0.3	No Hit
TTCCTGTACCGATCAATAGTGCGATGTGGGGACGGAGAAGGTTAGCTCAGCCAACTGTTGGATATGTTGGT	12	0.3	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTTTTTG	12	0.3	No Hit
CTCGTACAGAGGGAGCCCAAAGGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCA	12	0.3	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAA	10	0.25	No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATG	10	0.25	No Hit
TATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAA	10	0.25	No Hit
AATTGATACCGTAACTTCGGGAGAAGGTATGCCCCAAGTAGGTGAACTCGTACAGAGGGAGCCCAAAGGGG	9	0.22499999999999998	No Hit
CATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAA	9	0.22499999999999998	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACA	9	0.22499999999999998	No Hit
ACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTA	8	0.2	No Hit
GCCCAAAGGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTGCAAACACGAAA	8	0.2	No Hit
GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGT	8	0.2	No Hit
GCCCCAAGTAGGTGAACTCGTACAGAGGGAGCCCAAAGGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTA	8	0.2	No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACAATAA	8	0.2	No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAA	8	0.2	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGA	7	0.17500000000000002	No Hit
GACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGT	7	0.17500000000000002	No Hit
AACCGACACTGGTGCGCGAGATGAGTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAAAT	7	0.17500000000000002	No Hit
ATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTT	7	0.17500000000000002	No Hit
ATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTT	7	0.17500000000000002	No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAA	6	0.15	No Hit
GTGATACCCTGCTTCCAGGAAAAGCCACTAAGCTTCAGCTATTGACGACCGTACCGCAAACCGACACTGGT	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
ATAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGAT	6	0.15	No Hit
GTTTAATAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGACGCCTGCCCGGTGCTGG	6	0.15	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
TACAGAGGGAGCCCAAAGGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTG	6	0.15	No Hit
GTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCA	6	0.15	No Hit
GCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTTTTTGCAACCCCTTTGGGCTCCCTCTGTA	6	0.15	No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACGATAA	6	0.15	No Hit
CGTACAGAGGGAGCCCAAAGGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCACT	6	0.15	No Hit
ACCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTA	6	0.15	No Hit
TTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCA	5	0.125	No Hit
GTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACG	5	0.125	No Hit
GTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACAATAACGGTCCTAAGGTAGCGAAATTC	5	0.125	No Hit
CTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCG	5	0.125	No Hit
GATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCAT	5	0.125	No Hit
AGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATT	5	0.125	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCA	5	0.125	No Hit
ATTCCTGTACCGATCAATAGTGCGATGTGGGGACGGAGAAGGTTAGCTCAGCCAACTGTTGGATATGTTGG	5	0.125	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTA	5	0.125	No Hit
GTACATGGGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GAGGGAGCCCAAAGGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTGCAAA	5	0.125	No Hit
ACTTGGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCTGAGTTCTCTCAAG	5	0.125	No Hit
TAACAATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGA	5	0.125	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG	5	0.125	No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAACTATAA	5	0.125	No Hit
GTACGAGTTCACCTACTTGGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTC	5	0.125	No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATCGTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9701 READS because READLEN < 1
Read 9701 spots for SRR13209945.sra
Written 9701 spots for SRR13209945.sra
Rejected 9750 READS because READLEN < 1
Read 9750 spots for SRR13209945.sra
Written 9750 spots for SRR13209945.sra
SRR ids: ['SRR13209945.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_dst9ij1e
SRR13209945.sra spots: 620913
blocks: [[1, 9701], [9702, 19402], [19403, 29103], [29104, 38804], [38805, 48505], [48506, 58206], [58207, 67907], [67908, 77608], [77609, 87309], [87310, 97010], [97011, 106711], [106712, 116412], [116413, 126113], [126114, 135814], [135815, 145515], [145516, 155216], [155217, 164917], [164918, 174618], [174619, 184319], [184320, 194020], [194021, 203721], [203722, 213422], [213423, 223123], [223124, 232824], [232825, 242525], [242526, 252226], [252227, 261927], [261928, 271628], [271629, 281329], [281330, 291030], [291031, 300731], [300732, 310432], [310433, 320133], [320134, 329834], [329835, 339535], [339536, 349236], [349237, 358937], [358938, 368638], [368639, 378339], [378340, 388040], [388041, 397741], [397742, 407442], [407443, 417143], [417144, 426844], [426845, 436545], [436546, 446246], [446247, 455947], [455948, 465648], [465649, 475349], [475350, 485050], [485051, 494751], [494752, 504452], [504453, 514153], [514154, 523854], [523855, 533555], [533556, 543256], [543257, 552957], [552958, 562658], [562659, 572359], [572360, 582060], [582061, 591761], [591762, 601462], [601463, 611163], [611164, 620913]]
SRR13209945 file size 109836
SRR13209945 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13209945 SRR13209945_1.fastq
Input file:	SRR13209945_1.fastq
trimmed:	SRR13209945-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 17:14:05 2022 >> started

Fri May  6 17:14:06 2022 >> done (0.743s)
620913 reads processed; of these:
     0 ( 0.00%) short reads filtered out after trimming by size control
    13 ( 0.00%) empty reads filtered out after trimming by size control
620900 (100.00%) reads available; of these:
   544 ( 0.09%) trimmed reads available after processing
620356 (99.91%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 22	    12	  0.00%
 23	    23	  0.00%
 24	    16	  0.00%
 25	     5	  0.00%
 26	     7	  0.00%
 27	     5	  0.00%
 28	    15	  0.00%
 29	    14	  0.00%
 30	    32	  0.01%
 31	    47	  0.01%
 32	    57	  0.01%
 33	    84	  0.01%
 34	   149	  0.02%
 35	    88	  0.01%
 36	   120	  0.02%
 37	   115	  0.02%
 38	   181	  0.03%
 39	   169	  0.03%
 40	   189	  0.03%
 41	   223	  0.04%
 42	   174	  0.03%
 43	   194	  0.03%
 44	   227	  0.04%
 45	   241	  0.04%
 46	   229	  0.04%
 47	   267	  0.04%
 48	   278	  0.04%
 49	   315	  0.05%
 50	   264	  0.04%
 51	   306	  0.05%
 52	   308	  0.05%
 53	   370	  0.06%
 54	   286	  0.05%
 55	   309	  0.05%
 56	   382	  0.06%
 57	   391	  0.06%
 58	   396	  0.06%
 59	   440	  0.07%
 60	   501	  0.08%
 61	   457	  0.07%
 62	   522	  0.08%
 63	   481	  0.08%
 64	   456	  0.07%
 65	   624	  0.10%
 66	   789	  0.13%
 67	  2832	  0.46%
 68	 13674	  2.20%
 69	 44555	  7.18%
 70	140702	 22.66%
 71	408379	 65.77%
620900 reads passed initial QC


criterion=sequence-density
sequence-density=4.52
sequence-density-rank=1
fanout-score=1.93
fanout-score-rank=36
prefix-density=8.73
prefix-fanout=1.0
sequence=GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT


criterion=fanout-score
sequence-density=0.17
sequence-density-rank=35
fanout-score=47.59
fanout-score-rank=1
prefix-density=8.24
prefix-fanout=1.0
sequence=GTACATGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGAGTCCGGACGTTAGCACCCGGTGCTCTGTCTCCCAAGCTGTACTCTGCGGTATTCGGAGTTTGCATAGGTTTGGTAAGTCGCCATGACCCCCTAGCCTAAACAGTGCTCTACCCCCGCAGGTAATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCCCTAGTTTTGCAACACTAGTGGGTTCGGACCTCCAGTACCTGTTACGGCACCTTCATCCTGGCCATGGATAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGATTCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGGTTAACCTTGCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACCCTTGCGGGCTCCTACTTTTTGTAAGCATGCGGTTTCAGGATCTATTTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTAT -o SRR13209945 -
Input file:	STDIN
trimmed:	SRR13209945-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GAGTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGCATCATCACAAACATTTCAACTTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Fri May  6 17:14:12 2022 >> started

Fri May  6 17:14:13 2022 >> done (0.757s)
372540 reads processed; of these:
  8095 ( 2.17%) short reads filtered out after trimming by size control
  3094 ( 0.83%) empty reads filtered out after trimming by size control
361351 (97.00%) reads available; of these:
  8862 ( 2.45%) trimmed reads available after processing
352489 (97.55%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     8	  0.00%
 19	     3	  0.00%
 20	     5	  0.00%
 21	     2	  0.00%
 22	    14	  0.00%
 23	    37	  0.01%
 24	    12	  0.00%
 25	     9	  0.00%
 26	     7	  0.00%
 27	     4	  0.00%
 28	    14	  0.00%
 29	     8	  0.00%
 30	    29	  0.01%
 31	    23	  0.01%
 32	    36	  0.01%
 33	    62	  0.02%
 34	    86	  0.02%
 35	    76	  0.02%
 36	    69	  0.02%
 37	    72	  0.02%
 38	   103	  0.03%
 39	   114	  0.03%
 40	   121	  0.03%
 41	   136	  0.04%
 42	   100	  0.03%
 43	   126	  0.03%
 44	   129	  0.04%
 45	   154	  0.04%
 46	   139	  0.04%
 47	   169	  0.05%
 48	   148	  0.04%
 49	   185	  0.05%
 50	   161	  0.04%
 51	   189	  0.05%
 52	   196	  0.05%
 53	   226	  0.06%
 54	   183	  0.05%
 55	   175	  0.05%
 56	   231	  0.06%
 57	   239	  0.07%
 58	   265	  0.07%
 59	   261	  0.07%
 60	   385	  0.11%
 61	   279	  0.08%
 62	   335	  0.09%
 63	   294	  0.08%
 64	   345	  0.10%
 65	   547	  0.15%
 66	  1067	  0.30%
 67	  4720	  1.31%
 68	 12281	  3.40%
 69	 23520	  6.51%
 70	 77010	 21.31%
 71	236242	 65.38%


criterion=sequence-density
sequence-density=3.02
sequence-density-rank=1
fanout-score=3.01
fanout-score-rank=33
prefix-density=3.13
prefix-fanout=2.9
sequence=GTTACGGCCGCCGTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACAGTCGCAGCCACCGATTTTTTGCAACCCCTTTGGGCTCCCTCTGTACGAGTTCACCTACTTGGGGCATACCTTCTCCCGAAGTTACGGTATCAATTTGCCGAGTTCCTTCTCCTGAGTTCTCTCAAGCGCCTTAGAATACTCATCTCGCGCACCAGTGTCGGTTTGCGGTACGGTCGTCAATAGCTGAAGCTTAGTGGCTTTTCCTGGAAGCAGGGTATCACTCACTTCGTCTGCAAGCAGACTCGTTATCACCCCTCATCTAAGCCCGGCGGATTTGCCTACCAGGCACGACTACAGGCTTGAACCAACATATCCAACAGTTGGCTGAGCTAACCTTCTCCGTCCCCACATCGCACTATTGATCGGTACAGGAATATTGA


criterion=fanout-score
sequence-density=0.18
sequence-density-rank=34
fanout-score=40.15
fanout-score-rank=1
prefix-density=7.08
prefix-fanout=1.0
sequence=GTACATGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGAGTCCGGACGTTAGCACCCGGTGCTCTGTCTCCCAAGCTGTACTCTGCGGTATTCGGAGTTTGCATAGGTTTGGTAAGTCGCCATGACCCCCTAGCCTAAACAGTGCTCTACCCCCGCAGGTAATACTTGAGGCACTACCTAAATAGTTTTCGGAGAGAACCAGCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCCCCTAGTTTTGCAACACTAGTGGGTTCGGACCTCCAGTACCTGTTACGGCACCTTCATCCTGGCCATGGATAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGATTCGCCCTATTCGGACTCGATTTCTCTACGGCTTCCCTATTCGGTTAACCTTGCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCAGTCACCCTTGCGGGCTCCTACTTTTTGTAAGCATGCGGTTTCAGGATCTATTTC
                                 Started job on |	May 06 17:14:50
                             Started mapping on |	May 06 17:14:51
                                    Finished on |	May 06 17:15:09
       Mapping speed, Million of reads per hour |	121.94

                          Number of input reads |	609711
                      Average input read length |	70
                                    UNIQUE READS:
                   Uniquely mapped reads number |	110504
                        Uniquely mapped reads % |	18.12%
                          Average mapped length |	69.03
                       Number of splices: Total |	8614
            Number of splices: Annotated (sjdb) |	6166
                       Number of splices: GT/AG |	8333
                       Number of splices: GC/AG |	68
                       Number of splices: AT/AC |	5
               Number of splices: Non-canonical |	208
                      Mismatch rate per base, % |	0.67%
                         Deletion rate per base |	0.02%
                        Deletion average length |	1.33
                        Insertion rate per base |	0.04%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	117125
             % of reads mapped to multiple loci |	19.21%
        Number of reads mapped to too many loci |	7449
             % of reads mapped to too many loci |	1.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	61.36%
                     % of reads unmapped: other |	0.08%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	382082	382082	382082
N_multimapping	117125	117125	117125
N_noFeature	68299	88677	89351
N_ambiguous	1828	625	480
UnstrandedReadsAssigned:40377 PositiveStrandReadsAssigned:21202 NegativeStrandReadsAssigned:20673
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13209945 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13209945-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 609,711 reads, 73,916 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 685 rounds

 180870 SRR13209945.ke.tsv
  58302 SRR13209945.se.tsv
 239172 total
==> SRR13209945.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13209945.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13209945 completed mapping pipeline successfully
