Starting /dee2/code/volunteer_pipeline.sh SRR13210227
    current disk space = 2143500040
    free memory = 132458604 
SRR13210227 SRAfilesize
7bc1a22f9973724b43d0f554bdc304e7  SRR13210227.sra
SRR13210227.sra file validated
SRR13210227 is single end
SRR13210227 is conventional basespace
SRR13210227 read1 length is 42-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1814
Sequences flagged as poor quality	0
Sequence length	42-71
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.761852260198456	32.0	32.0	32.0	32.0	32.0
2	31.00826901874311	32.0	32.0	32.0	32.0	32.0
3	31.176957001102537	32.0	32.0	32.0	32.0	32.0
4	31.19845644983462	32.0	32.0	32.0	32.0	32.0
5	31.065600882028665	32.0	32.0	32.0	32.0	32.0
6	34.481256890848954	36.0	36.0	36.0	32.0	36.0
7	34.54906284454245	36.0	36.0	36.0	32.0	36.0
8	34.504961411245866	36.0	36.0	36.0	32.0	36.0
9	34.70396912899669	36.0	36.0	36.0	32.0	36.0
10	34.47905181918412	36.0	36.0	36.0	32.0	36.0
11	34.64222712238148	36.0	36.0	36.0	32.0	36.0
12	34.29988974641676	36.0	36.0	36.0	32.0	36.0
13	34.60749724366042	36.0	36.0	36.0	32.0	36.0
14	34.65214994487321	36.0	36.0	36.0	32.0	36.0
15	34.5909592061742	36.0	36.0	36.0	32.0	36.0
16	34.39029768467475	36.0	36.0	36.0	32.0	36.0
17	34.24586549062845	36.0	36.0	36.0	32.0	36.0
18	34.17916207276736	36.0	36.0	36.0	32.0	36.0
19	34.105843439911794	36.0	36.0	36.0	32.0	36.0
20	34.33131201764057	36.0	36.0	36.0	32.0	36.0
21	34.34895259095921	36.0	36.0	36.0	32.0	36.0
22	34.074421168687984	36.0	36.0	36.0	32.0	36.0
23	34.312568908489524	36.0	36.0	36.0	32.0	36.0
24	34.19955898566703	36.0	36.0	36.0	32.0	36.0
25	34.298235942668136	36.0	36.0	36.0	32.0	36.0
26	33.74586549062845	36.0	36.0	36.0	32.0	36.0
27	34.04189636163175	36.0	36.0	36.0	32.0	36.0
28	33.787761852260196	36.0	36.0	36.0	32.0	36.0
29	33.929988974641674	36.0	36.0	36.0	32.0	36.0
30	33.836824696802644	36.0	36.0	36.0	32.0	36.0
31	33.79933847850055	36.0	36.0	36.0	32.0	36.0
32	33.6284454244763	36.0	36.0	36.0	32.0	36.0
33	33.80760749724366	36.0	36.0	36.0	32.0	36.0
34	33.63836824696803	36.0	36.0	36.0	32.0	36.0
35	33.788313120176404	36.0	36.0	36.0	32.0	36.0
36	33.501653803748624	36.0	36.0	36.0	21.0	36.0
37	33.53914002205072	36.0	36.0	36.0	21.0	36.0
38	33.55071664829107	36.0	36.0	36.0	21.0	36.0
39	33.54465270121279	36.0	36.0	36.0	21.0	36.0
40	33.274531422271224	36.0	36.0	36.0	21.0	36.0
41	33.30154355016538	36.0	36.0	36.0	21.0	36.0
42	33.36549062844542	36.0	36.0	36.0	21.0	36.0
43	33.30446773303916	36.0	36.0	36.0	21.0	36.0
44	33.14183222958057	36.0	36.0	36.0	21.0	36.0
45	33.04583103257868	36.0	36.0	36.0	21.0	36.0
46	32.949723756906074	36.0	36.0	36.0	21.0	36.0
47	33.223880597014926	36.0	36.0	36.0	21.0	36.0
48	33.090657822001106	36.0	36.0	36.0	21.0	36.0
49	32.77446102819237	36.0	36.0	36.0	14.0	36.0
50	32.809839690436704	36.0	36.0	36.0	14.0	36.0
51	32.83582089552239	36.0	36.0	36.0	14.0	36.0
52	33.072495849474265	36.0	36.0	36.0	21.0	36.0
53	33.125692137320044	36.0	36.0	36.0	21.0	36.0
54	33.1218161683278	36.0	36.0	36.0	21.0	36.0
55	33.29568106312292	36.0	36.0	36.0	21.0	36.0
56	32.62126245847176	36.0	36.0	36.0	14.0	36.0
57	32.60221606648199	36.0	36.0	36.0	14.0	36.0
58	32.57372505543237	36.0	36.0	36.0	14.0	36.0
59	32.69345898004435	36.0	36.0	36.0	14.0	36.0
60	32.92286348501665	36.0	36.0	36.0	14.0	36.0
61	32.2857935627081	36.0	36.0	36.0	14.0	36.0
62	32.589672404219876	36.0	36.0	36.0	14.0	36.0
63	32.35722222222222	36.0	32.0	36.0	14.0	36.0
64	32.492213570634036	36.0	36.0	36.0	14.0	36.0
65	32.28754171301446	36.0	32.0	36.0	14.0	36.0
66	32.23260990539789	36.0	32.0	36.0	14.0	36.0
67	32.14031180400891	36.0	32.0	36.0	14.0	36.0
68	32.1976483762598	36.0	32.0	36.0	14.0	36.0
69	32.47395535203206	36.0	36.0	36.0	14.0	36.0
70	32.28891580860085	36.0	32.0	36.0	14.0	36.0
71	31.411371237458194	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	1.0
24	6.0
25	13.0
26	18.0
27	30.0
28	50.0
29	68.0
30	87.0
31	134.0
32	188.0
33	284.0
34	483.0
35	450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.60529217199559	17.03417861080485	13.065049614112459	26.295479603087102
2	15.600882028665932	21.664829106945977	35.0606394707828	27.67364939360529
3	17.805953693495038	27.56339581036384	26.405733186328558	28.224917309812568
4	11.57662624035281	15.821389195148841	35.11576626240353	37.48621830209482
5	12.183020948180816	36.71444321940463	33.0760749724366	18.02646085997795
6	34.61962513781698	35.777287761852264	16.75854465270121	12.844542447629548
7	31.422271223814775	30.209481808158767	20.562293274531424	17.805953693495038
8	29.823594266813668	29.60308710033076	19.845644983461963	20.727673649393605
9	30.154355016538037	13.395810363836825	19.40463065049614	37.045203969129
10	18.02646085997795	26.019845644983462	27.2877618522602	28.665931642778393
11	37.32083792723264	21.1135611907387	21.719955898566703	19.845644983461963
12	26.130099228224918	25.57883131201764	26.019845644983462	22.27122381477398
13	28.8864388092613	18.19184123484013	26.295479603087102	26.626240352811465
14	21.444321940463066	21.389195148842337	23.7045203969129	33.46196251378169
15	26.240352811466373	26.736493936052923	20.231532524807054	26.79162072767365
16	28.335170893054023	25.909592061742003	21.995589856670342	23.759647188533627
17	24.91730981256891	27.94928335170893	23.539140022050717	23.594266813671442
18	27.56339581036384	23.098125689084895	24.696802646085995	24.64167585446527
19	26.350606394707825	24.476295479603085	25.027563395810365	24.14553472987872
20	26.295479603087102	23.098125689084895	23.759647188533627	26.84674751929438
21	28.44542447629548	23.7045203969129	22.877618522601985	24.972436604189635
22	25.744211686879826	21.830209481808158	25.303197353914005	27.122381477398015
23	23.92502756339581	21.830209481808158	27.618522601984562	26.626240352811465
24	26.57111356119074	24.586549062844544	24.366041896361633	24.476295479603085
25	24.75192943770673	24.090407938257993	24.20066152149945	26.957001102535834
26	25.689084895259096	26.019845644983462	24.42116868798236	23.869900771775082
27	26.84674751929438	24.310915104740904	23.373759647188532	25.468577728776186
28	21.775082690187432	23.7045203969129	26.295479603087102	28.224917309812568
29	23.814773980154357	24.20066152149945	24.807056229327454	27.17750826901874
30	24.807056229327454	22.05071664829107	26.84674751929438	26.295479603087102
31	26.51598676957001	26.130099228224918	21.719955898566703	25.633958103638367
32	25.689084895259096	22.822491730981255	23.649393605292172	27.839029768467476
33	23.759647188533627	23.26350606394708	25.413450937155456	27.56339581036384
34	25.633958103638367	23.042998897464166	23.48401323042999	27.839029768467476
35	28.500551267916208	22.160970231532527	26.074972436604188	23.26350606394708
36	25.799338478500548	23.759647188533627	23.26350606394708	27.17750826901874
37	26.736493936052923	24.255788313120178	23.7045203969129	25.303197353914005
38	26.957001102535834	22.712238147739804	24.972436604189635	25.358324145534727
39	27.122381477398015	21.885336273428887	25.08269018743109	25.909592061742003
40	26.57111356119074	24.531422271223814	24.14553472987872	24.75192943770673
41	24.10369553226696	26.034197462768894	24.214009928295642	25.648097076668506
42	27.728776185226018	24.696802646085995	23.42888643880926	24.14553472987872
43	26.916712630998347	23.000551571980143	23.662437948152235	26.42029784886928
44	27.428256070640177	23.67549668874172	23.23399558498896	25.662251655629138
45	25.510767531750417	24.627277747101047	23.90944229707344	25.952512424075096
46	24.19889502762431	24.806629834254142	24.03314917127072	26.96132596685083
47	24.820342730790493	25.483692647871752	24.70978441127695	24.98618021006081
48	29.408512990602542	23.604201216141515	22.277501381978997	24.70978441127695
49	23.88059701492537	27.031509121061358	22.940851299060256	26.14704256495301
50	26.920950801547818	25.26257600884467	22.609176340519625	25.207296849087896
51	22.16694306246545	26.25760088446656	24.76506357103372	26.810392482034274
52	24.40509131156613	24.515771997786388	23.242944106253457	27.83619258439402
53	26.356589147286826	20.87486157253599	26.245847176079735	26.52270210409745
54	27.18715393133998	24.52934662236988	25.30454042081949	22.97895902547065
55	26.411960132890368	23.69878183831672	25.692137320044296	24.197120708748614
56	26.703601108033244	25.041551246537395	23.37950138504155	24.87534626038781
57	24.87534626038781	24.542936288088644	23.213296398891966	27.368421052631582
58	26.733222407099277	24.01552967276761	24.18191902384914	25.06932889628397
59	23.28159645232816	24.27937915742794	25.88691796008869	26.55210643015521
60	26.02663706992231	23.085460599334073	25.527192008879023	25.360710321864595
61	25.693673695893455	24.195338512763595	23.640399556048834	26.47058823529412
62	25.37479178234314	23.375902276513045	23.875624652970572	27.373681288173234
63	24.958310172317955	25.236242356864924	25.514174541411894	24.291272929405224
64	26.195773081201335	22.914349276974416	24.74972191323693	26.140155728587317
65	24.694104560622915	21.46829810901001	26.640711902113463	27.196885428253616
66	27.267668336115747	24.095715080690038	24.652198107957705	23.984418475236506
67	24.4988864142539	25.0	26.169265033407573	24.33184855233853
68	26.483762597984324	25.47592385218365	23.852183650615903	24.188129899216126
69	28.677733257012022	22.49570692615913	25.300515168860905	23.526044647967943
70	26.347668079951546	26.16596002422774	24.651726226529377	22.83464566929134
71	36.20401337792642	35.28428093645485	28.511705685618725	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	2.0
11	1.5
12	1.0
13	1.5
14	2.0
15	1.5
16	1.0
17	1.0
18	0.5
19	1.0
20	3.5
21	4.0
22	2.0
23	1.0
24	1.0
25	3.0
26	7.0
27	13.0
28	15.0
29	12.0
30	9.0
31	8.0
32	13.5
33	25.5
34	37.5
35	38.0
36	31.0
37	34.0
38	46.0
39	52.0
40	55.5
41	56.0
42	66.5
43	84.5
44	90.0
45	97.5
46	91.0
47	76.5
48	75.0
49	81.5
50	90.5
51	89.5
52	85.5
53	81.5
54	85.5
55	90.5
56	100.0
57	98.0
58	79.5
59	75.5
60	73.5
61	59.0
62	50.5
63	55.0
64	51.5
65	35.5
66	23.5
67	20.0
68	17.0
69	15.0
70	13.5
71	12.0
72	12.5
73	18.5
74	23.5
75	21.0
76	14.0
77	9.0
78	5.5
79	4.5
80	5.5
81	5.0
82	4.0
83	3.5
84	2.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.05512679162072767
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.05537098560354374
57	0.0
58	0.05543237250554324
59	0.0
60	0.0
61	0.0
62	0.0
63	0.05555555555555555
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	1.0
43	1.0
44	1.0
45	1.0
46	1.0
47	0.0
48	0.0
49	0.0
50	0.0
51	2.0
52	1.0
53	0.0
54	0.0
55	0.0
56	1.0
57	1.0
58	0.0
59	2.0
60	0.0
61	1.0
62	1.0
63	2.0
64	0.0
65	1.0
66	1.0
67	10.0
68	39.0
69	96.0
70	455.0
71	1196.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.70672546857773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44626021370208	79.32745314222713
2	6.34820867379007	11.13561190738699
3	2.262727844123193	5.953693495038589
4	0.6913890634820867	2.4255788313120177
5	0.18856065367693275	0.8269018743109152
6	0.06285355122564425	0.33076074972436603
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA	6	0.33076074972436603	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCG	5	0.27563395810363833	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCA	5	0.27563395810363833	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG	5	0.27563395810363833	No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT	4	0.2205071664829107	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	4	0.2205071664829107	No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATG	4	0.2205071664829107	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTT	4	0.2205071664829107	No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTAC	4	0.2205071664829107	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAA	4	0.2205071664829107	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG	4	0.2205071664829107	No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG	4	0.2205071664829107	No Hit
GTACATGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG	4	0.2205071664829107	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG	4	0.2205071664829107	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAA	4	0.2205071664829107	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGG	3	0.16538037486218302	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGT	3	0.16538037486218302	No Hit
ACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTA	3	0.16538037486218302	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG	3	0.16538037486218302	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT	3	0.16538037486218302	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGT	3	0.16538037486218302	No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	3	0.16538037486218302	No Hit
GTACTGGTACCAAAACAGATATATAGACCAATGGAACAGAACAGAGACCTCAGAAATAACACCACACATGT	3	0.16538037486218302	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTAT	3	0.16538037486218302	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCA	3	0.16538037486218302	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTG	3	0.16538037486218302	No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA	3	0.16538037486218302	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTA	3	0.16538037486218302	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	3	0.16538037486218302	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT	3	0.16538037486218302	No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA	3	0.16538037486218302	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTAC	3	0.16538037486218302	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAA	3	0.16538037486218302	No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATAT	3	0.16538037486218302	No Hit
AACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGCCGTGCGTACTTAGACATGCATGG	3	0.16538037486218302	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA	3	0.16538037486218302	No Hit
CGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAA	3	0.16538037486218302	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG	3	0.16538037486218302	No Hit
GATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTG	3	0.16538037486218302	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA	3	0.16538037486218302	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGT	3	0.16538037486218302	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT	3	0.16538037486218302	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGA	3	0.16538037486218302	No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGCCG	3	0.16538037486218302	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT	3	0.16538037486218302	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAG	3	0.16538037486218302	No Hit
GGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTAC	3	0.16538037486218302	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGC	3	0.16538037486218302	No Hit
GAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGT	3	0.16538037486218302	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAA	3	0.16538037486218302	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAG	3	0.16538037486218302	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGG	2	0.11025358324145534	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAA	2	0.11025358324145534	No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGT	2	0.11025358324145534	No Hit
CATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG	2	0.11025358324145534	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTT	2	0.11025358324145534	No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA	2	0.11025358324145534	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT	2	0.11025358324145534	No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTA	2	0.11025358324145534	No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCG	2	0.11025358324145534	No Hit
CATCAGCCACAGAGACTTTGGTGGTTGCACCCACAGTGTTTGTACAGAAATGGAGAAAAAAACAGGTG	2	0.11025358324145534	No Hit
GGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAACTACG	2	0.11025358324145534	No Hit
TAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC	2	0.11025358324145534	No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGG	2	0.11025358324145534	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	2	0.11025358324145534	No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	2	0.11025358324145534	No Hit
GTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGC	2	0.11025358324145534	No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTG	2	0.11025358324145534	No Hit
TTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGATGGCAG	2	0.11025358324145534	No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT	2	0.11025358324145534	No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGG	2	0.11025358324145534	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT	2	0.11025358324145534	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA	2	0.11025358324145534	No Hit
GAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTG	2	0.11025358324145534	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGC	2	0.11025358324145534	No Hit
TCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGC	2	0.11025358324145534	No Hit
ATTCTGCATGGGCCCCTGGGGCAGGGCTCCCATGGGCAGCGCCTGCATCAGCAGCGGGGTGGCCATGCGCA	2	0.11025358324145534	No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG	2	0.11025358324145534	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG	2	0.11025358324145534	No Hit
CCACTATACGGCTCCCAGCCCCTTCACGGGCGGCGCCGTTCATACATTAATGATATGGTTTTATCAGGTTT	2	0.11025358324145534	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAG	2	0.11025358324145534	No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG	2	0.11025358324145534	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2	0.11025358324145534	No Hit
GTCGTGTAGCTGCTAAGATATCATTTACTAGTCTTTCCTATCATCTTCAGAATATAGCCTGTTCAACAGAG	2	0.11025358324145534	No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA	2	0.11025358324145534	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	2	0.11025358324145534	No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGT	2	0.11025358324145534	No Hit
CTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCA	2	0.11025358324145534	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGA	2	0.11025358324145534	No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGAT	2	0.11025358324145534	No Hit
GACAAGAAGGGTTTTCCACCCTGGAATCAAGATGTCAGACTGGCTGGCTGCAGTGACGTGCACCTGTACT	2	0.11025358324145534	No Hit
CTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG	2	0.11025358324145534	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAA	2	0.11025358324145534	No Hit
GCTTAGCACCCGGCCAGCAGCTGTGCTCGACTTCTCGCCGGGCCTTAGCTGCCTCCCGGCGGGGCAGGG	2	0.11025358324145534	No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTA	2	0.11025358324145534	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCA	2	0.11025358324145534	No Hit
GGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA	2	0.11025358324145534	No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTT	2	0.11025358324145534	No Hit
GTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	2	0.11025358324145534	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAA	2	0.11025358324145534	No Hit
GAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGAAACCTCACC	2	0.11025358324145534	No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAAC	2	0.11025358324145534	No Hit
TCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAA	2	0.11025358324145534	No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGAC	2	0.11025358324145534	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCCGCCTT	2	0.11025358324145534	No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGT	2	0.11025358324145534	No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA	2	0.11025358324145534	No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCG	2	0.11025358324145534	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAAT	2	0.11025358324145534	No Hit
CACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCA	2	0.11025358324145534	No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG	2	0.11025358324145534	No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG	2	0.11025358324145534	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAA	2	0.11025358324145534	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA	2	0.11025358324145534	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTT	2	0.11025358324145534	No Hit
GGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGC	2	0.11025358324145534	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGG	2	0.11025358324145534	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATT	2	0.11025358324145534	No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACA	2	0.11025358324145534	No Hit
GCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTT	2	0.11025358324145534	No Hit
CCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGT	2	0.11025358324145534	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCC	2	0.11025358324145534	No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGA	2	0.11025358324145534	No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT	2	0.11025358324145534	No Hit
TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA	2	0.11025358324145534	No Hit
GTGCATGGCCGTTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAT	2	0.11025358324145534	No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA	2	0.11025358324145534	No Hit
TCCACAGATACTTCTCGTCATCCGTCATCTGGATGACCAAGGGCACGTTAAATACATCCTGGAGCCACTTT	2	0.11025358324145534	No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG	2	0.11025358324145534	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA	2	0.11025358324145534	No Hit
GCCAAAATGTGGTTGATAGGGACAGAGTTTGCCAGCGCGGAACTGAGCGGGGTATATGCTGAATCCATCCG	2	0.11025358324145534	No Hit
AAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	2	0.11025358324145534	No Hit
TTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA	2	0.11025358324145534	No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA	2	0.11025358324145534	No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCG	2	0.11025358324145534	No Hit
CACAGGACCAGGCTGCGAGAACAGCATCATCAGCATCATGCTATTACAATCCCAAACCATGGGGGTTTCT	2	0.11025358324145534	No Hit
AAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGA	2	0.11025358324145534	No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAG	2	0.11025358324145534	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCC	2	0.11025358324145534	No Hit
TTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGC	2	0.11025358324145534	No Hit
TCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGT	2	0.11025358324145534	No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTT	2	0.11025358324145534	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC	2	0.11025358324145534	No Hit
TATCACATGTGACATCTTCCATTCCATGGTCTCCGACAAAGATGACGTTGACACACCGATGCAGTTTTAGT	2	0.11025358324145534	No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCG	2	0.11025358324145534	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	2	0.11025358324145534	No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACG	2	0.11025358324145534	No Hit
CAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG	2	0.11025358324145534	No Hit
TCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGG	2	0.11025358324145534	No Hit
CATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTT	2	0.11025358324145534	No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTA	2	0.11025358324145534	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATG	2	0.11025358324145534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 28 READS because READLEN < 1
Read 28 spots for SRR13210227.sra
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Rejected 28 READS because READLEN < 1
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Rejected 28 READS because READLEN < 1
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Written 28 spots for SRR13210227.sra
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Written 50 spots for SRR13210227.sra
SRR ids: ['SRR13210227.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_s09zuu61
SRR13210227.sra spots: 1814
blocks: [[1, 28], [29, 56], [57, 84], [85, 112], [113, 140], [141, 168], [169, 196], [197, 224], [225, 252], [253, 280], [281, 308], [309, 336], [337, 364], [365, 392], [393, 420], [421, 448], [449, 476], [477, 504], [505, 532], [533, 560], [561, 588], [589, 616], [617, 644], [645, 672], [673, 700], [701, 728], [729, 756], [757, 784], [785, 812], [813, 840], [841, 868], [869, 896], [897, 924], [925, 952], [953, 980], [981, 1008], [1009, 1036], [1037, 1064], [1065, 1092], [1093, 1120], [1121, 1148], [1149, 1176], [1177, 1204], [1205, 1232], [1233, 1260], [1261, 1288], [1289, 1316], [1317, 1344], [1345, 1372], [1373, 1400], [1401, 1428], [1429, 1456], [1457, 1484], [1485, 1512], [1513, 1540], [1541, 1568], [1569, 1596], [1597, 1624], [1625, 1652], [1653, 1680], [1681, 1708], [1709, 1736], [1737, 1764], [1765, 1814]]
SRR13210227 file size 316
SRR13210227 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13210227 SRR13210227_1.fastq
Input file:	SRR13210227_1.fastq
trimmed:	SRR13210227-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 15:02:44 2022 >> started

Fri May  6 15:02:44 2022 >> done (0.006s)
1814 reads processed; of these:
   0 ( 0.00%) short reads filtered out after trimming by size control
   0 ( 0.00%) empty reads filtered out after trimming by size control
1814 (100.00%) reads available; of these:
1814 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 42	   1	  0.06%
 43	   1	  0.06%
 44	   1	  0.06%
 45	   1	  0.06%
 46	   1	  0.06%
 47	   0	  0.00%
 48	   0	  0.00%
 49	   0	  0.00%
 50	   0	  0.00%
 51	   2	  0.11%
 52	   1	  0.06%
 53	   0	  0.00%
 54	   0	  0.00%
 55	   0	  0.00%
 56	   1	  0.06%
 57	   1	  0.06%
 58	   0	  0.00%
 59	   2	  0.11%
 60	   0	  0.00%
 61	   1	  0.06%
 62	   1	  0.06%
 63	   2	  0.11%
 64	   0	  0.00%
 65	   1	  0.06%
 66	   1	  0.06%
 67	  10	  0.55%
 68	  39	  2.15%
 69	  96	  5.29%
 70	 455	 25.08%
 71	1196	 65.93%
1814 reads passed initial QC
                                 Started job on |	May 06 15:03:08
                             Started mapping on |	May 06 15:03:09
                                    Finished on |	May 06 15:03:12
       Mapping speed, Million of reads per hour |	2.18

                          Number of input reads |	1814
                      Average input read length |	70
                                    UNIQUE READS:
                   Uniquely mapped reads number |	656
                        Uniquely mapped reads % |	36.16%
                          Average mapped length |	69.24
                       Number of splices: Total |	65
            Number of splices: Annotated (sjdb) |	60
                       Number of splices: GT/AG |	64
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	1
                      Mismatch rate per base, % |	0.88%
                         Deletion rate per base |	0.04%
                        Deletion average length |	1.21
                        Insertion rate per base |	0.06%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1036
             % of reads mapped to multiple loci |	57.11%
        Number of reads mapped to too many loci |	3
             % of reads mapped to too many loci |	0.17%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.45%
                     % of reads unmapped: other |	0.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	122	122	122
N_multimapping	1036	1036	1036
N_noFeature	282	464	467
N_ambiguous	23	11	6
UnstrandedReadsAssigned:351 PositiveStrandReadsAssigned:181 NegativeStrandReadsAssigned:183
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13210227 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13210227-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,814 reads, 487 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 400 rounds

 180870 SRR13210227.ke.tsv
  58302 SRR13210227.se.tsv
 239172 total
==> SRR13210227.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13210227.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210227 completed mapping pipeline successfully
