Starting /dee2/code/volunteer_pipeline.sh SRR13210723
    current disk space = 2137503688
    free memory = 124223508 
SRR13210723 SRAfilesize
5edf13f0ff270864e5af5303a3a00cda  SRR13210723.sra
SRR13210723.sra file validated
SRR13210723 is paired end
SRR13210723 is conventional basespace
SRR13210723 read1 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210723_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22875	32.0	32.0	32.0	32.0	32.0
2	31.22525	32.0	32.0	32.0	32.0	32.0
3	31.25725	32.0	32.0	32.0	32.0	32.0
4	31.395	32.0	32.0	32.0	32.0	32.0
5	31.3515	32.0	32.0	32.0	32.0	32.0
6	34.8505	36.0	36.0	36.0	36.0	36.0
7	34.8805	36.0	36.0	36.0	36.0	36.0
8	34.92875	36.0	36.0	36.0	36.0	36.0
9	35.13975	36.0	36.0	36.0	36.0	36.0
10	34.776	36.0	36.0	36.0	32.0	36.0
11	34.934	36.0	36.0	36.0	36.0	36.0
12	34.9585	36.0	36.0	36.0	32.0	36.0
13	34.9855	36.0	36.0	36.0	36.0	36.0
14	34.9005	36.0	36.0	36.0	32.0	36.0
15	35.044	36.0	36.0	36.0	36.0	36.0
16	34.933	36.0	36.0	36.0	32.0	36.0
17	34.9695	36.0	36.0	36.0	36.0	36.0
18	34.943	36.0	36.0	36.0	36.0	36.0
19	34.776	36.0	36.0	36.0	32.0	36.0
20	34.90225	36.0	36.0	36.0	32.0	36.0
21	34.95475	36.0	36.0	36.0	32.0	36.0
22	34.8815	36.0	36.0	36.0	32.0	36.0
23	34.734	36.0	36.0	36.0	32.0	36.0
24	34.738	36.0	36.0	36.0	32.0	36.0
25	34.69025	36.0	36.0	36.0	32.0	36.0
26	34.6845	36.0	36.0	36.0	32.0	36.0
27	34.58475	36.0	36.0	36.0	32.0	36.0
28	34.77325	36.0	36.0	36.0	32.0	36.0
29	34.57525	36.0	36.0	36.0	32.0	36.0
30	34.6165	36.0	36.0	36.0	32.0	36.0
31	34.73475	36.0	36.0	36.0	32.0	36.0
32	34.50675	36.0	36.0	36.0	32.0	36.0
33	33.901	36.0	36.0	36.0	32.0	36.0
34	32.05675	36.0	36.0	36.0	14.0	36.0
35	23.4415	36.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	4.0
24	5.0
25	10.0
26	22.0
27	38.0
28	48.0
29	77.0
30	131.0
31	166.0
32	324.0
33	617.0
34	1136.0
35	1422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4	18.9	11.600000000000001	27.1
2	18.525	20.175	36.5	24.8
3	17.525	25.674999999999997	28.000000000000004	28.799999999999997
4	10.925	16.875	37.925	34.275
5	15.75	35.6	31.374999999999996	17.275
6	32.44933700275207	36.85263947960971	16.887665749311985	13.810357768326245
7	28.775000000000002	30.075000000000003	21.45	19.7
8	26.900000000000002	30.85	20.225	22.025
9	27.725	13.875000000000002	20.75	37.65
10	18.3	24.45	28.249999999999996	28.999999999999996
11	37.574999999999996	21.575	20.925	19.925
12	26.05	25.275	25.5	23.175
13	28.525	17.4	25.474999999999998	28.599999999999998
14	24.7	20.9	24.224999999999998	30.175
15	27.125	23.925	21.825	27.125
16	26.5	24.425	22.175	26.900000000000002
17	26.150000000000002	24.275	24.0	25.575
18	27.05	23.075000000000003	25.45	24.425
19	28.025	22.275	23.799999999999997	25.900000000000002
20	27.125	22.75	22.925	27.200000000000003
21	31.1	22.0	22.25	24.65
22	28.825	22.025	22.75	26.400000000000002
23	24.075	22.45	24.375	29.099999999999998
24	24.775	25.775	23.200000000000003	26.25
25	26.745058794095574	21.165874405804352	24.568426319739807	27.52064048036027
26	27.34550913184889	24.568426319739807	23.942957217913435	24.143107330497873
27	27.83479349186483	22.478097622027533	21.576971214017522	28.11013767209011
28	25.41906429822367	23.39254440830623	25.769326995246434	25.41906429822367
29	26.22088655146506	22.33909341347358	24.117205108940645	27.32281492612071
30	24.405506883604506	24.055068836045056	26.758448060075096	24.780976220275345
31	27.909887359198997	22.60325406758448	23.103879849812266	26.382978723404253
32	24.962330487192368	23.556002009040682	23.556002009040682	27.92566549472627
33	25.26181353767561	23.678160919540232	24.597701149425287	26.462324393358877
34	26.922032056506385	25.23770714479761	24.341211627275197	23.49904917142081
35	38.24521934758155	34.19572553430822	27.559055118110237	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	2.0
10	1.5
11	1.0
12	1.0
13	1.5
14	2.0
15	2.0
16	2.5
17	3.0
18	3.0
19	8.5
20	14.0
21	12.5
22	11.0
23	11.0
24	26.5
25	42.0
26	42.0
27	55.5
28	69.0
29	69.0
30	100.0
31	131.0
32	131.0
33	143.5
34	156.0
35	156.0
36	166.0
37	176.0
38	176.0
39	234.5
40	293.0
41	298.0
42	303.0
43	303.0
44	328.5
45	354.0
46	354.0
47	352.0
48	350.0
49	350.0
50	336.5
51	323.0
52	323.0
53	317.5
54	312.0
55	312.0
56	323.0
57	334.0
58	334.0
59	316.5
60	299.0
61	271.0
62	243.0
63	243.0
64	222.0
65	201.0
66	201.0
67	188.5
68	176.0
69	176.0
70	117.0
71	58.0
72	58.0
73	47.0
74	36.0
75	36.0
76	29.0
77	22.0
78	22.0
79	29.0
80	36.0
81	23.5
82	11.0
83	11.0
84	11.0
85	11.0
86	11.0
87	12.0
88	13.0
89	13.0
90	11.0
91	9.0
92	9.0
93	8.5
94	8.0
95	8.0
96	4.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.075
26	0.075
27	0.125
28	0.075
29	0.17500000000000002
30	0.125
31	0.125
32	0.44999999999999996
33	2.125
34	7.9750000000000005
35	33.324999999999996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.13493253373314	53.449999999999996
2	9.407796101949026	12.55
3	4.6476761619190405	9.3
4	1.6116941529235385	4.3
5	1.199400299850075	4.0
6	0.9745127436281859	3.9
7	0.5622188905547226	2.625
8	0.6746626686656672	3.5999999999999996
9	0.07496251874062969	0.44999999999999996
>10	0.712143928035982	5.825
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG	17	0.42500000000000004	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	16	0.4	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAG	15	0.375	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCN	14	0.35000000000000003	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	14	0.35000000000000003	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCG	14	0.35000000000000003	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT	14	0.35000000000000003	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACN	13	0.325	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATG	13	0.325	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	11	0.27499999999999997	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	11	0.27499999999999997	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTN	11	0.27499999999999997	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA	10	0.25	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCAT	10	0.25	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCG	10	0.25	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTT	10	0.25	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	10	0.25	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGT	10	0.25	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAA	10	0.25	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA	9	0.22499999999999998	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTA	9	0.22499999999999998	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTN	8	0.2	No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGN	8	0.2	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGG	8	0.2	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAA	8	0.2	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCN	8	0.2	No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTAN	8	0.2	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGANN	8	0.2	No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCAN	8	0.2	No Hit
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAA	8	0.2	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGA	8	0.2	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAAT	8	0.2	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	8	0.2	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGG	8	0.2	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGT	8	0.2	No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTN	8	0.2	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATN	8	0.2	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTT	8	0.2	No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCG	8	0.2	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTNN	7	0.17500000000000002	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGT	7	0.17500000000000002	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	7	0.17500000000000002	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCN	7	0.17500000000000002	No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTAN	7	0.17500000000000002	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	7	0.17500000000000002	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTT	7	0.17500000000000002	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG	7	0.17500000000000002	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGG	7	0.17500000000000002	No Hit
GCACCAGACTTGCCCTCCAATGGATCCTCGTTAAA	7	0.17500000000000002	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGN	7	0.17500000000000002	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGG	7	0.17500000000000002	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCAT	7	0.17500000000000002	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGT	7	0.17500000000000002	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG	7	0.17500000000000002	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCN	6	0.15	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAG	6	0.15	No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCN	6	0.15	No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT	6	0.15	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCN	6	0.15	No Hit
CTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTT	6	0.15	No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGA	6	0.15	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	6	0.15	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGG	6	0.15	No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAG	6	0.15	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA	6	0.15	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTG	6	0.15	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCNNN	6	0.15	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG	6	0.15	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAG	6	0.15	No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAA	6	0.15	No Hit
TGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCN	6	0.15	No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGNN	6	0.15	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGN	6	0.15	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT	6	0.15	No Hit
CATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT	6	0.15	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGA	6	0.15	No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGA	6	0.15	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGA	6	0.15	No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGT	6	0.15	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	6	0.15	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG	5	0.125	No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCN	5	0.125	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCG	5	0.125	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCG	5	0.125	No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTT	5	0.125	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	5	0.125	No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCG	5	0.125	No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCA	5	0.125	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG	5	0.125	No Hit
CAAATTACCCACTCCCGACCCGGGGAGGTAGTGAN	5	0.125	No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGNN	5	0.125	No Hit
ATCATGGCCTCAGTTCCGAAAACCAACAAAATAGA	5	0.125	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACA	5	0.125	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTG	5	0.125	No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCN	5	0.125	No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATA	5	0.125	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT	5	0.125	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAA	5	0.125	No Hit
TCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTT	5	0.125	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA	5	0.125	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGN	5	0.125	No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCA	5	0.125	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG	5	0.125	No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACNN	5	0.125	No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG	5	0.125	No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGT	5	0.125	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGN	5	0.125	No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTT	5	0.125	No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG	5	0.125	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCG	5	0.125	No Hit
GAGCTGGAATTACCGCGGCTGCTGGCACCAGACTT	5	0.125	No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR13210723 read2 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210723_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98425	32.0	32.0	32.0	32.0	32.0
2	30.433	32.0	32.0	32.0	32.0	32.0
3	30.616	32.0	32.0	32.0	32.0	32.0
4	30.6395	32.0	32.0	32.0	32.0	32.0
5	30.484	32.0	32.0	32.0	32.0	32.0
6	34.1305	36.0	36.0	36.0	32.0	36.0
7	33.97375	36.0	36.0	36.0	32.0	36.0
8	34.11775	36.0	36.0	36.0	32.0	36.0
9	34.3245	36.0	36.0	36.0	32.0	36.0
10	33.81425	36.0	36.0	36.0	32.0	36.0
11	34.0645	36.0	36.0	36.0	32.0	36.0
12	34.04675	36.0	36.0	36.0	32.0	36.0
13	34.1655	36.0	36.0	36.0	32.0	36.0
14	34.04925	36.0	36.0	36.0	32.0	36.0
15	34.16925	36.0	36.0	36.0	32.0	36.0
16	33.9995	36.0	36.0	36.0	32.0	36.0
17	33.9975	36.0	36.0	36.0	32.0	36.0
18	34.09525	36.0	36.0	36.0	32.0	36.0
19	33.999	36.0	36.0	36.0	32.0	36.0
20	33.847	36.0	36.0	36.0	32.0	36.0
21	33.90525	36.0	36.0	36.0	32.0	36.0
22	33.83275	36.0	36.0	36.0	32.0	36.0
23	33.803	36.0	36.0	36.0	32.0	36.0
24	33.91325	36.0	36.0	36.0	32.0	36.0
25	33.859	36.0	36.0	36.0	32.0	36.0
26	33.8665	36.0	36.0	36.0	32.0	36.0
27	33.764	36.0	36.0	36.0	27.0	36.0
28	33.70975	36.0	36.0	36.0	27.0	36.0
29	33.92225	36.0	36.0	36.0	32.0	36.0
30	33.68	36.0	36.0	36.0	27.0	36.0
31	33.94525	36.0	36.0	36.0	32.0	36.0
32	33.55925	36.0	36.0	36.0	27.0	36.0
33	33.089	36.0	36.0	36.0	21.0	36.0
34	31.1355	36.0	36.0	36.0	14.0	36.0
35	22.7045	36.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	0.0
18	5.0
19	2.0
20	3.0
21	9.0
22	17.0
23	22.0
24	35.0
25	37.0
26	64.0
27	74.0
28	114.0
29	131.0
30	162.0
31	269.0
32	406.0
33	612.0
34	985.0
35	1050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.405418966382335	18.364274962368288	10.712493728048168	26.517812343201204
2	18.61688799799549	21.598596842896516	35.30443497870208	24.480080180405913
3	17.329332333083272	26.481620405101275	28.882220555138783	27.306826706676667
4	12.528132033008252	15.55388847211803	35.708927231807955	36.209052263065765
5	16.775000000000002	36.8	30.2	16.225
6	33.5	35.975	17.075000000000003	13.450000000000001
7	30.075000000000003	30.8	21.75	17.375
8	28.23911955977989	30.06503251625813	20.135067533766886	21.5607803901951
9	29.35733933483371	14.303575893973495	18.904726181545385	37.43435858964741
10	17.03351675837919	25.76288144072036	27.43871935967984	29.764882441220607
11	39.65	21.075	20.95	18.325
12	27.375	22.95	26.5	23.175
13	29.37937937937938	18.093093093093092	24.624624624624623	27.902902902902905
14	23.799999999999997	21.375	24.75	30.075000000000003
15	28.253253253253252	24.924924924924923	20.42042042042042	26.401401401401404
16	27.55	25.05	21.375	26.025
17	25.825	25.124999999999996	24.85	24.2
18	27.725	22.05	24.55	25.674999999999997
19	27.33183295823956	22.080520130032507	24.33108277069267	26.25656414103526
20	27.33866933466733	23.08654327163582	22.98649324662331	26.588294147073537
21	30.430430430430434	22.07207207207207	23.34834834834835	24.14914914914915
22	27.34550913184889	23.46760070052539	22.34175631723793	26.845133850387793
23	24.480600750938674	23.27909887359199	24.005006257822277	28.235294117647058
24	25.56334501752629	24.887330996494743	22.58387581372058	26.965448172258387
25	24.705882352941178	24.60575719649562	23.27909887359199	27.40926157697122
26	25.720010017530683	25.219133483596295	25.97044828449787	23.09040821437516
27	27.265898848272407	21.75763645468202	23.335002503755632	27.641462193289932
28	25.913870806209317	22.183274912368553	25.53830746119179	26.364546820230345
29	25.663495242864297	22.283425137706562	25.41311967951928	26.639959939909865
30	25.80160320641283	23.697394789579157	25.0751503006012	25.425851703406817
31	28.93681043129388	22.24172517552658	23.1444332998997	25.67703109327984
32	24.77386934673367	23.09045226130653	22.512562814070353	29.623115577889447
33	25.146646263708238	23.31038000510074	25.044631471563378	26.498342259627645
34	28.91369452763409	23.49578001633542	24.4214538524367	23.169071603593792
35	40.41916167664671	33.98203592814371	25.59880239520958	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	2.0
4	2.5
5	3.0
6	3.0
7	3.5
8	4.0
9	4.0
10	2.0
11	0.0
12	0.0
13	1.5
14	3.0
15	3.0
16	5.5
17	8.0
18	8.0
19	10.0
20	12.0
21	16.5
22	21.0
23	21.0
24	27.5
25	34.0
26	34.0
27	47.0
28	60.0
29	60.0
30	96.0
31	132.0
32	132.0
33	131.5
34	131.0
35	131.0
36	158.5
37	186.0
38	186.0
39	244.5
40	303.0
41	287.5
42	272.0
43	272.0
44	332.5
45	393.0
46	393.0
47	359.0
48	325.0
49	325.0
50	345.0
51	365.0
52	365.0
53	330.0
54	295.0
55	295.0
56	301.0
57	307.0
58	307.0
59	298.0
60	289.0
61	284.5
62	280.0
63	280.0
64	229.0
65	178.0
66	178.0
67	173.0
68	168.0
69	168.0
70	114.5
71	61.0
72	61.0
73	49.0
74	37.0
75	37.0
76	35.5
77	34.0
78	34.0
79	30.0
80	26.0
81	21.0
82	16.0
83	16.0
84	15.5
85	15.0
86	15.0
87	13.0
88	11.0
89	11.0
90	9.5
91	8.0
92	8.0
93	8.0
94	8.0
95	8.0
96	4.0
97	0.0
98	0.0
99	6.0
100	12.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.35000000000000003
2	0.22499999999999998
3	0.025
4	0.025
5	0.0
6	0.0
7	0.0
8	0.05
9	0.025
10	0.05
11	0.0
12	0.0
13	0.1
14	0.0
15	0.1
16	0.0
17	0.0
18	0.0
19	0.025
20	0.05
21	0.1
22	0.075
23	0.125
24	0.15
25	0.125
26	0.17500000000000002
27	0.15
28	0.15
29	0.15
30	0.2
31	0.3
32	0.5
33	1.975
34	8.175
35	33.2
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.1
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41678726483357	56.95
2	8.5383502170767	11.799999999999999
3	3.400868306801737	7.049999999999999
4	1.9536903039073805	5.4
5	1.1215629522431259	3.875
6	0.8321273516642547	3.45
7	0.47033285094066574	2.275
8	0.1447178002894356	0.8
9	0.361794500723589	2.25
>10	0.759768451519537	6.15
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTAATATACGCTATTGGAGCTGGAATTACCGCG	15	0.375	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCAT	14	0.35000000000000003	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	14	0.35000000000000003	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAG	13	0.325	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTN	13	0.325	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT	13	0.325	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCG	12	0.3	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTN	12	0.3	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTG	12	0.3	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	12	0.3	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG	11	0.27499999999999997	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACN	11	0.27499999999999997	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	11	0.27499999999999997	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	11	0.27499999999999997	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAG	11	0.27499999999999997	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGT	11	0.27499999999999997	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTN	10	0.25	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAA	10	0.25	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT	10	0.25	No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCN	10	0.25	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	10	0.25	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCG	9	0.22499999999999998	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGG	9	0.22499999999999998	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	9	0.22499999999999998	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCN	9	0.22499999999999998	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCN	9	0.22499999999999998	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTNN	9	0.22499999999999998	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	9	0.22499999999999998	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGN	9	0.22499999999999998	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAA	9	0.22499999999999998	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG	9	0.22499999999999998	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACA	8	0.2	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG	8	0.2	No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACNN	8	0.2	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGT	8	0.2	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG	7	0.17500000000000002	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCNNN	7	0.17500000000000002	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCN	7	0.17500000000000002	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	7	0.17500000000000002	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCN	7	0.17500000000000002	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTG	7	0.17500000000000002	No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGN	7	0.17500000000000002	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAA	7	0.17500000000000002	No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGG	7	0.17500000000000002	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTA	7	0.17500000000000002	No Hit
TTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTT	7	0.17500000000000002	No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATN	7	0.17500000000000002	No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGG	7	0.17500000000000002	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	6	0.15	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCN	6	0.15	No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCN	6	0.15	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA	6	0.15	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGG	6	0.15	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCG	6	0.15	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGNN	6	0.15	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATNN	6	0.15	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG	6	0.15	No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGN	6	0.15	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATG	6	0.15	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA	6	0.15	No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAA	6	0.15	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGG	6	0.15	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGT	6	0.15	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	6	0.15	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG	6	0.15	No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCAN	6	0.15	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	6	0.15	No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTN	6	0.15	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCANNN	6	0.15	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTT	6	0.15	No Hit
GATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGG	6	0.15	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATG	5	0.125	No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGNN	5	0.125	No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA	5	0.125	No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAG	5	0.125	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA	5	0.125	No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	5	0.125	No Hit
ATACGAATGCCCCCGGCCGTCCCTCTTAATCATGG	5	0.125	No Hit
GCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT	5	0.125	No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	5	0.125	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	5	0.125	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAN	5	0.125	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGN	5	0.125	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTT	5	0.125	No Hit
CGTTTATGGTCGGAACTACGACGGTATCTGATCGT	5	0.125	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGANN	5	0.125	No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGAT	5	0.125	No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA	5	0.125	No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGN	5	0.125	No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCN	5	0.125	No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAA	5	0.125	No Hit
CATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT	5	0.125	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTT	5	0.125	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGN	5	0.125	No Hit
ATGTATTACTGTGCGAGAGATGTCTACGGTGAAGG	5	0.125	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGG	5	0.125	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGA	5	0.125	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATN	5	0.125	No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTT	5	0.125	No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATN	5	0.125	No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCG	5	0.125	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14089 spots for SRR13210723.sra
Written 14089 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
Read 14063 spots for SRR13210723.sra
Written 14063 spots for SRR13210723.sra
SRR ids: ['SRR13210723.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_dacnuxk5
SRR13210723.sra spots: 900058
blocks: [[1, 14063], [14064, 28126], [28127, 42189], [42190, 56252], [56253, 70315], [70316, 84378], [84379, 98441], [98442, 112504], [112505, 126567], [126568, 140630], [140631, 154693], [154694, 168756], [168757, 182819], [182820, 196882], [196883, 210945], [210946, 225008], [225009, 239071], [239072, 253134], [253135, 267197], [267198, 281260], [281261, 295323], [295324, 309386], [309387, 323449], [323450, 337512], [337513, 351575], [351576, 365638], [365639, 379701], [379702, 393764], [393765, 407827], [407828, 421890], [421891, 435953], [435954, 450016], [450017, 464079], [464080, 478142], [478143, 492205], [492206, 506268], [506269, 520331], [520332, 534394], [534395, 548457], [548458, 562520], [562521, 576583], [576584, 590646], [590647, 604709], [604710, 618772], [618773, 632835], [632836, 646898], [646899, 660961], [660962, 675024], [675025, 689087], [689088, 703150], [703151, 717213], [717214, 731276], [731277, 745339], [745340, 759402], [759403, 773465], [773466, 787528], [787529, 801591], [801592, 815654], [815655, 829717], [829718, 843780], [843781, 857843], [857844, 871906], [871907, 885969], [885970, 900058]]
SRR13210723 file size 97348
SRR13210723 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13210723 SRR13210723_1.fastq SRR13210723_2.fastq
Input file:	SRR13210723_1.fastq
Paired file:	SRR13210723_2.fastq
trimmed:	SRR13210723-trimmed-pair1.fastq, SRR13210723-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Fri May  6 15:51:18 2022 >> started

Fri May  6 15:51:20 2022 >> done (1.809s)
900058 read pairs processed; of these:
     1 ( 0.00%) short read pairs filtered out after trimming by size control
     1 ( 0.00%) empty read pairs filtered out after trimming by size control
900056 (100.00%) read pairs available; of these:
497359 (55.26%) trimmed read pairs available after processing
402697 (44.74%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 21	     1	  0.00%
 22	     0	  0.00%
 23	     2	  0.00%
 24	     2	  0.00%
 25	     6	  0.00%
 26	     8	  0.00%
 27	    10	  0.00%
 28	    26	  0.00%
 29	   125	  0.01%
 30	   839	  0.09%
 31	   567	  0.06%
 32	  5252	  0.58%
 33	 64421	  7.16%
 34	426100	 47.34%
 35	402697	 44.74%
900056 reads passed initial QC


criterion=sequence-density
sequence-density=0.86
sequence-density-rank=1
fanout-score=3.46
fanout-score-rank=33
prefix-density=2.98
prefix-fanout=1.0
sequence=GAGTACATGGGG


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=33
fanout-score=75.18
fanout-score-rank=1
prefix-density=2.76
prefix-fanout=1.0
sequence=GTACATGGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGCCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTG


criterion=sequence-density
sequence-density=0.90
sequence-density-rank=1
fanout-score=3.38
fanout-score-rank=33
prefix-density=3.02
prefix-fanout=1.0
sequence=GAGTACATGGGG


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=34
fanout-score=79.82
fanout-score-rank=1
prefix-density=2.81
prefix-fanout=1.0
sequence=GTACATGGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTG
SRR13210723 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 06 15:52:21
                             Started mapping on |	May 06 15:52:21
                                    Finished on |	May 06 15:52:44
       Mapping speed, Million of reads per hour |	140.88

                          Number of input reads |	900056
                      Average input read length |	53
                                    UNIQUE READS:
                   Uniquely mapped reads number |	225629
                        Uniquely mapped reads % |	25.07%
                          Average mapped length |	52.36
                       Number of splices: Total |	14857
            Number of splices: Annotated (sjdb) |	14032
                       Number of splices: GT/AG |	14364
                       Number of splices: GC/AG |	121
                       Number of splices: AT/AC |	26
               Number of splices: Non-canonical |	346
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.10
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	508930
             % of reads mapped to multiple loci |	56.54%
        Number of reads mapped to too many loci |	6757
             % of reads mapped to too many loci |	0.75%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	17.44%
                     % of reads unmapped: other |	0.19%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	165497	165497	165497
N_multimapping	508930	508930	508930
N_noFeature	70583	146892	146216
N_ambiguous	7493	2263	2415
UnstrandedReadsAssigned:147553 PositiveStrandReadsAssigned:76474 NegativeStrandReadsAssigned:76998
Dataset is classified unstranded
MeadianReadLen=27 20thPercentileLength=26 echo kmer=21
SRR13210723 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 21
[index] number of targets: 180,869
[index] number of k-mers: 103,451,480
[index] number of equivalence classes: 935,577
[quant] running in paired-end mode
[quant] will process pair 1: SRR13210723-trimmed-pair1.fastq
                             SRR13210723-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 900,056 reads, 300,262 reads pseudoaligned
[quant] estimated average fragment length: 162.991
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 910 rounds

 180870 SRR13210723.ke.tsv
  58302 SRR13210723.se.tsv
 239172 total
==> SRR13210723.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	10	0	0
ENST00000448914.1	13	1	0	0
ENST00000415118.1	8	9	0	0
ENST00000631435.1	12	13	0	0
ENST00000632684.1	12	13	0	0
ENST00000454908.1	17	2.42857	0	0
ENST00000390567.1	20	2.11111	0	0
ENST00000603326.1	19	2.85714	0	0
ENST00000414852.1	16	3.33333	0	0

==> SRR13210723.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210723 completed mapping pipeline successfully
