Starting /dee2/code/volunteer_pipeline.sh SRR13210713
    current disk space = 1769129556012
    free memory = 349789100 
SRR13210713 SRAfilesize
6d2d7756485265dfa5c666dff939f2bd  SRR13210713.sra
SRR13210713.sra file validated
SRR13210713 is paired end
SRR13210713 is conventional basespace
SRR13210713 read1 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210713_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8615	32.0	32.0	32.0	32.0	32.0
2	30.89825	32.0	32.0	32.0	32.0	32.0
3	30.86475	32.0	32.0	32.0	32.0	32.0
4	31.0765	32.0	32.0	32.0	32.0	32.0
5	31.0525	32.0	32.0	32.0	32.0	32.0
6	34.609	36.0	36.0	36.0	32.0	36.0
7	34.51725	36.0	36.0	36.0	32.0	36.0
8	34.64475	36.0	36.0	36.0	32.0	36.0
9	34.75	36.0	36.0	36.0	32.0	36.0
10	34.49	36.0	36.0	36.0	32.0	36.0
11	34.657	36.0	36.0	36.0	32.0	36.0
12	34.6225	36.0	36.0	36.0	32.0	36.0
13	34.647	36.0	36.0	36.0	32.0	36.0
14	34.61425	36.0	36.0	36.0	32.0	36.0
15	34.598	36.0	36.0	36.0	32.0	36.0
16	34.57725	36.0	36.0	36.0	32.0	36.0
17	34.53325	36.0	36.0	36.0	32.0	36.0
18	34.6595	36.0	36.0	36.0	32.0	36.0
19	34.30775	36.0	36.0	36.0	32.0	36.0
20	34.41875	36.0	36.0	36.0	32.0	36.0
21	34.382	36.0	36.0	36.0	32.0	36.0
22	34.3315	36.0	36.0	36.0	32.0	36.0
23	34.166	36.0	36.0	36.0	32.0	36.0
24	34.2875	36.0	36.0	36.0	32.0	36.0
25	34.135	36.0	36.0	36.0	32.0	36.0
26	34.00575	36.0	36.0	36.0	32.0	36.0
27	34.02875	36.0	36.0	36.0	32.0	36.0
28	33.82425	36.0	36.0	36.0	32.0	36.0
29	33.85	36.0	36.0	36.0	32.0	36.0
30	33.7945	36.0	36.0	36.0	32.0	36.0
31	33.98925	36.0	36.0	36.0	32.0	36.0
32	33.7535	36.0	36.0	36.0	32.0	36.0
33	33.4735	36.0	36.0	36.0	27.0	36.0
34	31.239	36.0	36.0	36.0	14.0	36.0
35	23.844	36.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	3.0
23	3.0
24	12.0
25	26.0
26	30.0
27	54.0
28	95.0
29	139.0
30	170.0
31	258.0
32	367.0
33	671.0
34	1064.0
35	1108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.625	18.9	11.625	24.85
2	13.5	24.375	33.925	28.199999999999996
3	19.825	29.2	29.475	21.5
4	13.65	17.625	35.575	33.15
5	14.45	36.775000000000006	30.2	18.575
6	32.21610805402702	31.065532766383193	18.234117058529264	18.48424212106053
7	26.05	29.675	22.725	21.55
8	29.2	29.875	21.675	19.25
9	30.075000000000003	13.600000000000001	20.474999999999998	35.85
10	20.3	24.975	28.375	26.35
11	32.725	23.775	20.825	22.675
12	26.8	28.000000000000004	25.174999999999997	20.025000000000002
13	28.999999999999996	18.275	23.575	29.15
14	23.65	22.85	24.9	28.599999999999998
15	31.474999999999998	20.7	21.45	26.375
16	21.675	23.875	31.0	23.45
17	29.45	25.724999999999998	24.55	20.275000000000002
18	29.4	19.25	27.35	24.0
19	27.05	26.8	25.6	20.549999999999997
20	25.474999999999998	22.2	26.775	25.55
21	25.474999999999998	26.25	26.8	21.475
22	27.575	25.525	18.075	28.825
23	19.375	28.4	23.549999999999997	28.675
24	23.724999999999998	31.924999999999997	22.75	21.6
25	25.35	24.075	20.375	30.2
26	24.05	26.575	24.474999999999998	24.9
27	27.020265198899175	21.491118338754063	20.31523642732049	31.17338003502627
28	22.91718789091819	26.419814861145856	25.869402051538653	24.7935951963973
29	26.901901901901905	18.343343343343342	26.976976976976978	27.77777777777778
30	22.62828535669587	25.882352941176475	31.11389236545682	20.375469336670836
31	33.41677096370463	19.849812265331664	18.798498122653317	27.934918648310386
32	18.78605467770253	24.680210684725356	26.00953097567093	30.52420366190118
33	23.016476552598224	24.334600760456272	28.77059569074778	23.878326996197718
34	23.862405200433372	25.65005417118093	27.546045503791984	22.941495124593718
35	29.193433261955747	39.47180585296217	31.334760885082087	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	4.0
2	2.0
3	2.0
4	7.0
5	12.0
6	12.0
7	10.5
8	9.0
9	9.0
10	6.0
11	3.0
12	3.0
13	4.5
14	6.0
15	6.0
16	7.5
17	9.0
18	9.0
19	12.0
20	15.0
21	18.0
22	21.0
23	21.0
24	26.0
25	31.0
26	31.0
27	35.5
28	40.0
29	40.0
30	56.0
31	72.0
32	72.0
33	87.0
34	102.0
35	102.0
36	111.0
37	120.0
38	120.0
39	166.0
40	212.0
41	239.5
42	267.0
43	267.0
44	400.0
45	533.0
46	533.0
47	685.5
48	838.0
49	838.0
50	713.5
51	589.0
52	589.0
53	402.0
54	215.0
55	215.0
56	203.5
57	192.0
58	192.0
59	184.0
60	176.0
61	173.5
62	171.0
63	171.0
64	141.5
65	112.0
66	112.0
67	113.0
68	114.0
69	114.0
70	75.5
71	37.0
72	37.0
73	31.0
74	25.0
75	25.0
76	24.5
77	24.0
78	24.0
79	19.5
80	15.0
81	11.0
82	7.0
83	7.0
84	7.5
85	8.0
86	8.0
87	6.0
88	4.0
89	4.0
90	5.0
91	6.0
92	6.0
93	5.5
94	5.0
95	5.0
96	3.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.075
28	0.075
29	0.1
30	0.125
31	0.125
32	0.325
33	1.375
34	7.7
35	29.95
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.47500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94330633635064	42.475
2	8.956646021915198	9.4
3	3.2872796569795137	5.175
4	2.1438780371605524	4.5
5	0.9528346831824679	2.5
6	0.7622677465459743	2.4
7	0.5240590757503573	1.925
8	0.6193425440686041	2.6
9	0.19056693663649357	0.8999999999999999
>10	1.2386850881372082	11.875
>50	0.28585040495474034	9.575
>100	0.09528346831824679	6.675000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	154	3.85	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	113	2.825	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	96	2.4	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	68	1.7000000000000002	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	63	1.575	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	53	1.325	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAT	52	1.3	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	51	1.275	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGT	42	1.05	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT	34	0.8500000000000001	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	32	0.8	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGNN	29	0.7250000000000001	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTNN	25	0.625	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA	25	0.625	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA	23	0.575	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	21	0.525	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGT	21	0.525	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	20	0.5	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	18	0.44999999999999996	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCA	16	0.4	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGA	15	0.375	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	15	0.375	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTANN	14	0.35000000000000003	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	13	0.325	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATAN	13	0.325	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCANN	13	0.325	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCN	12	0.3	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	12	0.3	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG	11	0.27499999999999997	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	11	0.27499999999999997	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCA	10	0.25	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCN	10	0.25	No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	10	0.25	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACN	9	0.22499999999999998	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATG	9	0.22499999999999998	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCG	9	0.22499999999999998	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACAT	9	0.22499999999999998	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTN	8	0.2	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACN	8	0.2	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATG	8	0.2	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	8	0.2	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGN	8	0.2	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	8	0.2	No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA	8	0.2	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8	0.2	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTA	8	0.2	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTN	8	0.2	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTANN	8	0.2	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGA	8	0.2	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG	8	0.2	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAG	7	0.17500000000000002	No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG	7	0.17500000000000002	No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAA	7	0.17500000000000002	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCAT	7	0.17500000000000002	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCN	7	0.17500000000000002	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA	7	0.17500000000000002	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGT	7	0.17500000000000002	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAG	7	0.17500000000000002	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT	7	0.17500000000000002	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	7	0.17500000000000002	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCAT	7	0.17500000000000002	No Hit
GCGCAATACGAATGCCCCCGGCCGTCCCTCTTAAT	6	0.15	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCN	6	0.15	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTT	6	0.15	No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT	6	0.15	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGG	6	0.15	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGT	6	0.15	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTAN	6	0.15	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG	6	0.15	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG	6	0.15	No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	6	0.15	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	6	0.15	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT	6	0.15	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.15	No Hit
CATGGAAAGCAGTGGTATCAACGCAGAGTACATGG	6	0.15	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTT	6	0.15	No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCG	6	0.15	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG	5	0.125	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG	5	0.125	No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA	5	0.125	No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATA	5	0.125	No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA	5	0.125	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAA	5	0.125	No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	5	0.125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGN	5	0.125	No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGN	5	0.125	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATG	5	0.125	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGG	5	0.125	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	5	0.125	No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGG	5	0.125	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGG	5	0.125	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGT	5	0.125	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGA	5	0.125	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTA	5	0.125	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.025	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.025	0.0	0.0	0.0	0.0
21	0.025	0.0	0.0	0.0	0.0
22	0.05	0.0	0.0	0.0	0.0
23	0.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR13210713 read2 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210713_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.899	32.0	32.0	32.0	32.0	32.0
2	30.639	32.0	32.0	32.0	32.0	32.0
3	30.7435	32.0	32.0	32.0	32.0	32.0
4	30.82275	32.0	32.0	32.0	32.0	32.0
5	30.61825	32.0	32.0	32.0	32.0	32.0
6	34.1665	36.0	36.0	36.0	32.0	36.0
7	34.02775	36.0	36.0	36.0	32.0	36.0
8	34.14225	36.0	36.0	36.0	32.0	36.0
9	34.16	36.0	36.0	36.0	32.0	36.0
10	34.03875	36.0	36.0	36.0	32.0	36.0
11	34.20725	36.0	36.0	36.0	32.0	36.0
12	34.0215	36.0	36.0	36.0	32.0	36.0
13	34.0675	36.0	36.0	36.0	32.0	36.0
14	33.976	36.0	36.0	36.0	32.0	36.0
15	34.19425	36.0	36.0	36.0	32.0	36.0
16	33.98125	36.0	36.0	36.0	32.0	36.0
17	33.9385	36.0	36.0	36.0	32.0	36.0
18	33.884	36.0	36.0	36.0	32.0	36.0
19	33.856	36.0	36.0	36.0	32.0	36.0
20	33.88325	36.0	36.0	36.0	32.0	36.0
21	33.75725	36.0	36.0	36.0	32.0	36.0
22	33.771	36.0	36.0	36.0	32.0	36.0
23	33.5815	36.0	36.0	36.0	27.0	36.0
24	33.71625	36.0	36.0	36.0	32.0	36.0
25	33.59925	36.0	36.0	36.0	27.0	36.0
26	33.57525	36.0	36.0	36.0	27.0	36.0
27	33.5395	36.0	36.0	36.0	27.0	36.0
28	33.4295	36.0	36.0	36.0	21.0	36.0
29	33.3695	36.0	36.0	36.0	21.0	36.0
30	33.3925	36.0	36.0	36.0	21.0	36.0
31	33.532	36.0	36.0	36.0	27.0	36.0
32	33.088	36.0	36.0	36.0	21.0	36.0
33	32.78225	36.0	36.0	36.0	14.0	36.0
34	30.65525	36.0	32.0	36.0	14.0	36.0
35	22.73775	32.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	3.0
17	3.0
18	3.0
19	9.0
20	5.0
21	10.0
22	17.0
23	13.0
24	29.0
25	52.0
26	64.0
27	79.0
28	106.0
29	142.0
30	199.0
31	254.0
32	392.0
33	683.0
34	990.0
35	946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.0440881763527	18.061122244488978	11.397795591182366	23.49699398797595
2	14.089089089089088	22.17217217217217	37.06206206206206	26.676676676676674
3	18.529632408102024	32.38309577394349	27.031757939484873	22.05551387846962
4	12.278069517379345	18.0545136284071	33.18329582395599	36.48412103025757
5	13.3	39.574999999999996	29.95	17.175
6	32.2	29.4	20.95	17.45
7	27.425	29.4	23.325000000000003	19.85
8	30.825000000000003	31.25	20.25	17.675
9	31.075000000000003	14.025000000000002	20.075000000000003	34.825
10	19.775000000000002	27.85	27.224999999999998	25.15
11	31.7	25.874999999999996	20.424999999999997	22.0
12	29.475	26.974999999999998	25.224999999999998	18.325
13	29.625	17.275	21.95	31.15
14	24.875	24.85	23.825	26.450000000000003
15	34.1	19.55	21.025	25.324999999999996
16	21.675	23.974999999999998	33.675	20.674999999999997
17	31.225	23.25	24.6	20.925
18	30.9	20.125	26.450000000000003	22.525000000000002
19	27.05	26.0	28.349999999999998	18.6
20	25.45	24.099999999999998	26.575	23.875
21	26.450000000000003	25.474999999999998	28.9	19.175
22	26.875	23.425	18.625	31.075000000000003
23	18.375	29.275000000000002	23.775	28.575
24	23.325000000000003	33.275	21.125	22.275
25	24.125	24.075	19.275000000000002	32.525
26	27.224999999999998	22.525000000000002	24.775	25.474999999999998
27	26.0	22.05	19.45	32.5
28	23.549999999999997	28.349999999999998	23.95	24.15
29	27.35	18.125	27.500000000000004	27.025
30	22.075	28.15	30.4	19.375
31	33.800000000000004	19.400000000000002	19.525000000000002	27.275
32	17.81781781781782	22.6976976976977	28.953953953953953	30.53053053053053
33	22.528445006321114	27.02907711757269	26.548672566371685	23.893805309734514
34	21.73913043478261	24.27761274642182	27.815284904131786	26.167971914663784
35	28.298485940879598	40.735400144196106	30.9661139149243	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	2.0
4	6.5
5	11.0
6	11.0
7	9.5
8	8.0
9	8.0
10	4.5
11	1.0
12	1.0
13	1.5
14	2.0
15	2.0
16	2.0
17	2.0
18	2.0
19	10.0
20	18.0
21	19.0
22	20.0
23	20.0
24	21.0
25	22.0
26	22.0
27	35.5
28	49.0
29	49.0
30	71.5
31	94.0
32	94.0
33	97.0
34	100.0
35	100.0
36	118.5
37	137.0
38	137.0
39	176.0
40	215.0
41	245.0
42	275.0
43	275.0
44	423.5
45	572.0
46	572.0
47	718.5
48	865.0
49	865.0
50	700.0
51	535.0
52	535.0
53	365.0
54	195.0
55	195.0
56	189.0
57	183.0
58	183.0
59	177.5
60	172.0
61	159.5
62	147.0
63	147.0
64	138.0
65	129.0
66	129.0
67	112.0
68	95.0
69	95.0
70	73.5
71	52.0
72	52.0
73	36.5
74	21.0
75	21.0
76	19.5
77	18.0
78	18.0
79	19.0
80	20.0
81	17.0
82	14.0
83	14.0
84	12.5
85	11.0
86	11.0
87	7.5
88	4.0
89	4.0
90	3.0
91	2.0
92	2.0
93	3.5
94	5.0
95	5.0
96	2.5
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.2
2	0.1
3	0.025
4	0.025
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.1
33	1.125
34	7.425
35	30.65
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45774326176335	45.125
2	8.451347647327546	9.25
3	2.8780264961169486	4.725
4	1.8730013704888075	4.1000000000000005
5	1.0507080858839652	2.875
6	0.7766103243490179	2.55
7	0.4111466423024212	1.575
8	0.1827318410232983	0.8
9	0.31978072179077205	1.575
>10	1.3704888076747372	13.475000000000001
>50	0.09136592051164916	2.9000000000000004
>100	0.13704888076747374	11.05
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	233	5.825	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	105	2.625	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	104	2.6	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	60	1.5	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	56	1.4000000000000001	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	43	1.075	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAT	37	0.9249999999999999	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA	31	0.775	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGT	28	0.7000000000000001	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT	26	0.65	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	26	0.65	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACN	24	0.6	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	23	0.575	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGNN	22	0.5499999999999999	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	18	0.44999999999999996	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTNN	17	0.42500000000000004	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTANN	17	0.42500000000000004	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA	16	0.4	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	16	0.4	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	16	0.4	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACAT	16	0.4	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	16	0.4	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCANN	14	0.35000000000000003	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGT	13	0.325	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG	12	0.3	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	12	0.3	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTANN	12	0.3	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	11	0.27499999999999997	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTN	11	0.27499999999999997	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCA	11	0.27499999999999997	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	11	0.27499999999999997	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCN	10	0.25	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	10	0.25	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATN	10	0.25	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAG	9	0.22499999999999998	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCAT	9	0.22499999999999998	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTG	9	0.22499999999999998	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATG	9	0.22499999999999998	No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCA	9	0.22499999999999998	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCG	9	0.22499999999999998	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT	9	0.22499999999999998	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	8	0.2	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGG	8	0.2	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGT	8	0.2	No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCN	7	0.17500000000000002	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATG	7	0.17500000000000002	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGT	7	0.17500000000000002	No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCN	7	0.17500000000000002	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCG	7	0.17500000000000002	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG	7	0.17500000000000002	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATAN	7	0.17500000000000002	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGN	7	0.17500000000000002	No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT	7	0.17500000000000002	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA	6	0.15	No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT	6	0.15	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGG	6	0.15	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGA	6	0.15	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	6	0.15	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCN	6	0.15	No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTAN	6	0.15	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCNNN	6	0.15	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
TATCAGATCAAAACCAACCCGGTCAGCCCCTCTCN	6	0.15	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCN	6	0.15	No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCN	6	0.15	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTA	6	0.15	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	6	0.15	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATA	6	0.15	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCAT	6	0.15	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTG	6	0.15	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG	5	0.125	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAG	5	0.125	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATG	5	0.125	No Hit
GCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCA	5	0.125	No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAG	5	0.125	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACA	5	0.125	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCN	5	0.125	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGNN	5	0.125	No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTAT	5	0.125	No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGAN	5	0.125	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATNN	5	0.125	No Hit
GCTAATACATGCCGACGGGCGCTGACCCCCTTCGN	5	0.125	No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCN	5	0.125	No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCAN	5	0.125	No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGN	5	0.125	No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGG	5	0.125	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATA	5	0.125	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	5	0.125	No Hit
ATCCAACTACGAGCTTTTTAACTGCAGCAACTTTA	5	0.125	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGT	5	0.125	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAA	5	0.125	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGA	5	0.125	No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.025	0.0	0.0	0.0	0.0
21	0.025	0.0	0.0	0.0	0.0
22	0.025	0.0	0.0	0.0	0.0
23	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCCAT	55	0.0034337132	19.973776	29
>>END_MODULE
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
Read 6987 spots for SRR13210713.sra
Written 6987 spots for SRR13210713.sra
Read 6976 spots for SRR13210713.sra
Written 6976 spots for SRR13210713.sra
SRR ids: ['SRR13210713.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9jrcytft
SRR13210713.sra spots: 334859
blocks: [[1, 6976], [6977, 13952], [13953, 20928], [20929, 27904], [27905, 34880], [34881, 41856], [41857, 48832], [48833, 55808], [55809, 62784], [62785, 69760], [69761, 76736], [76737, 83712], [83713, 90688], [90689, 97664], [97665, 104640], [104641, 111616], [111617, 118592], [118593, 125568], [125569, 132544], [132545, 139520], [139521, 146496], [146497, 153472], [153473, 160448], [160449, 167424], [167425, 174400], [174401, 181376], [181377, 188352], [188353, 195328], [195329, 202304], [202305, 209280], [209281, 216256], [216257, 223232], [223233, 230208], [230209, 237184], [237185, 244160], [244161, 251136], [251137, 258112], [258113, 265088], [265089, 272064], [272065, 279040], [279041, 286016], [286017, 292992], [292993, 299968], [299969, 306944], [306945, 313920], [313921, 320896], [320897, 327872], [327873, 334859]]
SRR13210713 file size 32768
SRR13210713 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13210713 SRR13210713_1.fastq SRR13210713_2.fastq
Input file:	SRR13210713_1.fastq
Paired file:	SRR13210713_2.fastq
trimmed:	SRR13210713-trimmed-pair1.fastq, SRR13210713-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Fri May  6 12:04:45 2022 >> started

Fri May  6 12:04:45 2022 >> done (0.406s)
334859 read pairs processed; of these:
     2 ( 0.00%) short read pairs filtered out after trimming by size control
     2 ( 0.00%) empty read pairs filtered out after trimming by size control
334855 (100.00%) read pairs available; of these:
174405 (52.08%) trimmed read pairs available after processing
160450 (47.92%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 23	     1	  0.00%
 24	     0	  0.00%
 25	     5	  0.00%
 26	     5	  0.00%
 27	     1	  0.00%
 28	    10	  0.00%
 29	    40	  0.01%
 30	   163	  0.05%
 31	   124	  0.04%
 32	  1269	  0.38%
 33	 17810	  5.32%
 34	154977	 46.28%
 35	160450	 47.92%
334855 reads passed initial QC


criterion=sequence-density
sequence-density=0.73
sequence-density-rank=1
fanout-score=18.26
fanout-score-rank=21
prefix-density=13.35
prefix-fanout=1.0
sequence=CCACTGCTTCCATGTACTCT


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=35
fanout-score=250.20
fanout-score-rank=1
prefix-density=11.88
prefix-fanout=1.0
sequence=AGTACATGGAAAGCAGTGGTA


criterion=sequence-density
sequence-density=0.79
sequence-density-rank=1
fanout-score=15.77
fanout-score-rank=26
prefix-density=12.30
prefix-fanout=1.0
sequence=GAGTACATGGGG


criterion=fanout-score
sequence-density=0.09
sequence-density-rank=32
fanout-score=218.26
fanout-score-rank=1
prefix-density=18.73
prefix-fanout=1.1
sequence=AGCAGTGGTATAAACGCAGAGTA
SRR13210713 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 06 12:05:39
                             Started mapping on |	May 06 12:05:40
                                    Finished on |	May 06 12:05:59
       Mapping speed, Million of reads per hour |	63.45

                          Number of input reads |	334855
                      Average input read length |	45
                                    UNIQUE READS:
                   Uniquely mapped reads number |	41351
                        Uniquely mapped reads % |	12.35%
                          Average mapped length |	43.21
                       Number of splices: Total |	2355
            Number of splices: Annotated (sjdb) |	2333
                       Number of splices: GT/AG |	2340
                       Number of splices: GC/AG |	11
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	1
                      Mismatch rate per base, % |	0.35%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.50
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	107997
             % of reads mapped to multiple loci |	32.25%
        Number of reads mapped to too many loci |	2779
             % of reads mapped to too many loci |	0.83%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	54.12%
                     % of reads unmapped: other |	0.45%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	185509	185509	185509
N_multimapping	107997	107997	107997
N_noFeature	17066	28997	28936
N_ambiguous	1000	256	279
UnstrandedReadsAssigned:23285 PositiveStrandReadsAssigned:12098 NegativeStrandReadsAssigned:12136
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13210713 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 180,869
[index] number of k-mers: 102,188,183
[index] number of equivalence classes: 1,184,234
[quant] running in paired-end mode
[quant] will process pair 1: SRR13210713-trimmed-pair1.fastq
                             SRR13210713-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 334,855 reads, 73,405 reads pseudoaligned
[quant] estimated average fragment length: 100.394
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 788 rounds

 180870 SRR13210713.ke.tsv
  58302 SRR13210713.se.tsv
 239172 total
==> SRR13210713.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	10	0	0
ENST00000448914.1	13	14	0	0
ENST00000415118.1	8	9	0	0
ENST00000631435.1	12	13	0	0
ENST00000632684.1	12	13	0	0
ENST00000454908.1	17	2.14286	0	0
ENST00000390567.1	20	2.71429	0	0
ENST00000603326.1	19	2.52632	0	0
ENST00000414852.1	16	1.6	0	0

==> SRR13210713.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210713 completed mapping pipeline successfully
