Starting /dee2/code/volunteer_pipeline.sh SRR13211489
    current disk space = 1773694772328
    free memory = 336314680 
SRR13211489 SRAfilesize
a8d2e4fafb5bb06fca9f753a8d9d63bd  SRR13211489.sra
SRR13211489.sra file validated
SRR13211489 is single end
SRR13211489 is conventional basespace
SRR13211489 read1 length is 40-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13211489_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	40-71
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50875	32.0	32.0	32.0	32.0	32.0
2	31.53425	32.0	32.0	32.0	32.0	32.0
3	31.66475	32.0	32.0	32.0	32.0	32.0
4	31.785	32.0	32.0	32.0	32.0	32.0
5	31.73425	32.0	32.0	32.0	32.0	32.0
6	35.46775	36.0	36.0	36.0	36.0	36.0
7	35.435	36.0	36.0	36.0	36.0	36.0
8	35.35075	36.0	36.0	36.0	36.0	36.0
9	35.5495	36.0	36.0	36.0	36.0	36.0
10	35.3505	36.0	36.0	36.0	36.0	36.0
11	35.3785	36.0	36.0	36.0	36.0	36.0
12	35.2065	36.0	36.0	36.0	36.0	36.0
13	35.2655	36.0	36.0	36.0	36.0	36.0
14	35.21775	36.0	36.0	36.0	36.0	36.0
15	35.10725	36.0	36.0	36.0	36.0	36.0
16	35.0515	36.0	36.0	36.0	36.0	36.0
17	34.95175	36.0	36.0	36.0	32.0	36.0
18	34.94025	36.0	36.0	36.0	32.0	36.0
19	35.36975	36.0	36.0	36.0	36.0	36.0
20	35.393	36.0	36.0	36.0	36.0	36.0
21	35.50925	36.0	36.0	36.0	36.0	36.0
22	35.463	36.0	36.0	36.0	36.0	36.0
23	35.386	36.0	36.0	36.0	36.0	36.0
24	35.344	36.0	36.0	36.0	36.0	36.0
25	35.24975	36.0	36.0	36.0	36.0	36.0
26	35.287	36.0	36.0	36.0	36.0	36.0
27	35.0875	36.0	36.0	36.0	36.0	36.0
28	34.67775	36.0	36.0	36.0	32.0	36.0
29	34.32	36.0	36.0	36.0	32.0	36.0
30	34.63425	36.0	36.0	36.0	32.0	36.0
31	34.881	36.0	36.0	36.0	32.0	36.0
32	35.17975	36.0	36.0	36.0	36.0	36.0
33	35.3425	36.0	36.0	36.0	36.0	36.0
34	35.2645	36.0	36.0	36.0	36.0	36.0
35	35.16775	36.0	36.0	36.0	36.0	36.0
36	35.1255	36.0	36.0	36.0	36.0	36.0
37	34.9395	36.0	36.0	36.0	32.0	36.0
38	34.50275	36.0	36.0	36.0	32.0	36.0
39	33.713	36.0	36.0	36.0	32.0	36.0
40	33.1385	36.0	36.0	36.0	14.0	36.0
41	32.78844711177794	36.0	36.0	36.0	14.0	36.0
42	32.351587896974245	36.0	32.0	36.0	14.0	36.0
43	33.18729682420605	36.0	36.0	36.0	14.0	36.0
44	34.32908227056764	36.0	36.0	36.0	32.0	36.0
45	35.03925981495374	36.0	36.0	36.0	36.0	36.0
46	35.00925231307827	36.0	36.0	36.0	36.0	36.0
47	35.0657664416104	36.0	36.0	36.0	36.0	36.0
48	34.77969492373093	36.0	36.0	36.0	36.0	36.0
49	34.448224112056025	36.0	36.0	36.0	32.0	36.0
50	34.090295147573784	36.0	36.0	36.0	32.0	36.0
51	34.495497748874435	36.0	36.0	36.0	32.0	36.0
52	34.778083562672	36.0	36.0	36.0	36.0	36.0
53	34.91541541541542	36.0	36.0	36.0	36.0	36.0
54	34.94943679599499	36.0	36.0	36.0	36.0	36.0
55	34.991737606409615	36.0	36.0	36.0	36.0	36.0
56	34.713927855711425	36.0	36.0	36.0	36.0	36.0
57	34.50400801603207	36.0	36.0	36.0	32.0	36.0
58	34.37509396141318	36.0	36.0	36.0	32.0	36.0
59	33.78220551378446	36.0	36.0	36.0	32.0	36.0
60	33.67911757332665	36.0	36.0	36.0	27.0	36.0
61	33.27019568489714	36.0	36.0	36.0	21.0	36.0
62	33.02736630680392	36.0	36.0	36.0	14.0	36.0
63	32.78729281767956	36.0	36.0	36.0	14.0	36.0
64	33.938190954773866	36.0	36.0	36.0	32.0	36.0
65	34.744221105527636	36.0	36.0	36.0	32.0	36.0
66	34.7697914048756	36.0	36.0	36.0	32.0	36.0
67	34.79929577464789	36.0	36.0	36.0	36.0	36.0
68	34.78746525145312	36.0	36.0	36.0	32.0	36.0
69	34.712509712509714	36.0	36.0	36.0	32.0	36.0
70	34.511313465783665	36.0	36.0	36.0	32.0	36.0
71	32.798906677079266	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	3.0
24	5.0
25	5.0
26	10.0
27	26.0
28	36.0
29	54.0
30	83.0
31	124.0
32	197.0
33	398.0
34	872.0
35	2186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.925	16.6	10.75	25.724999999999998
2	17.675	18.975	36.05	27.3
3	19.525000000000002	23.5	25.874999999999996	31.1
4	13.525	14.549999999999999	33.275	38.65
5	16.175	34.9	30.599999999999998	18.325
6	38.24561403508772	32.907268170426065	14.486215538847116	14.360902255639097
7	32.074999999999996	28.15	20.45	19.325
8	29.825000000000003	29.525000000000002	18.3	22.35
9	27.125	12.55	17.849999999999998	42.475
10	17.575	22.675	27.325	32.425
11	41.15	19.875	20.349999999999998	18.625
12	27.55	22.3	24.3	25.85
13	30.85	17.1	24.125	27.925
14	25.75	18.975	21.15	34.125
15	25.650000000000002	23.325000000000003	21.55	29.475
16	30.025000000000002	22.425	21.0	26.55
17	25.624999999999996	24.8	22.375	27.200000000000003
18	27.900000000000002	21.675	23.025000000000002	27.400000000000002
19	28.925	21.825	23.575	25.674999999999997
20	29.25	19.575	21.775	29.4
21	31.2	21.375	21.75	25.674999999999997
22	30.0	20.075000000000003	22.95	26.974999999999998
23	25.70642660665166	20.80520130032508	24.131032758189548	29.35733933483371
24	26.950000000000003	23.425	21.975	27.650000000000002
25	26.8	21.099999999999998	22.425	29.675
26	26.275	25.3	23.925	24.5
27	27.250000000000004	22.575	21.75	28.425
28	25.324999999999996	20.349999999999998	26.450000000000003	27.875
29	25.275	22.15	25.85	26.724999999999998
30	24.8	21.625	25.5	28.075
31	28.299999999999997	23.175	21.2	27.325
32	26.150000000000002	21.675	22.650000000000002	29.525000000000002
33	26.525	20.525	23.575	29.375
34	27.625	20.125	24.375	27.875
35	29.625	21.65	23.275000000000002	25.45
36	25.95	22.675	22.675	28.7
37	28.775000000000002	22.425	23.3	25.5
38	26.825	22.275	26.224999999999998	24.675
39	28.275	20.7	22.275	28.749999999999996
40	28.999999999999996	23.1	23.225	24.675
41	25.937968984492244	26.063031515757878	23.861930965482742	24.137068534267133
42	28.77157868401301	24.893670252689517	21.641230923192396	24.69352014010508
43	26.920190142606952	21.46609957468101	24.36827620715537	27.24543407555667
44	24.88122030507627	22.48062015503876	23.730932733183295	28.907226806701676
45	26.281570392598148	22.705676419104776	24.256064016004	26.756689172293076
46	27.306826706676667	22.80570142535634	23.355838959739934	26.531632908227053
47	27.53188297074269	22.305576394098527	24.90622655663916	25.256314078519633
48	33.05826456614154	23.280820205051263	20.705176294073517	22.95573893473368
49	26.23811905952976	23.911955977988995	22.861430715357677	26.988494247123562
50	25.76288144072036	23.936968484242122	22.086043021510758	28.214107053526767
51	26.163081540770385	22.761380690345174	22.786393196598297	28.289144572286144
52	25.519139354515886	23.067300475356518	24.54340755566675	26.870152614460846
53	29.679679679679676	20.02002002002002	23.973973973973976	26.326326326326328
54	29.837296620775973	21.802252816020026	24.180225281602002	24.180225281602002
55	28.067100650976464	22.208312468703053	24.76214321482223	24.962443665498245
56	28.707414829659317	22.62024048096192	21.8436873747495	26.828657314629258
57	25.501002004008015	22.895791583166332	20.415831663326653	31.187374749498996
58	27.211225256827866	20.621398145828113	24.931094963668254	27.23628163367577
59	25.93984962406015	24.93734335839599	22.105263157894736	27.017543859649123
60	26.322386563048383	22.887941840060165	23.965906242165957	26.82376535472549
61	26.24184646261917	25.23833416959358	20.672353236327147	27.84746613146011
62	25.48330404217926	23.273914135074065	22.596033140848608	28.646748681898064
63	25.439477649422397	24.91210447011552	24.535409342039177	25.113008538422903
64	27.110552763819097	22.512562814070353	21.758793969849247	28.618090452261306
65	27.51256281407035	22.43718592964824	23.417085427135678	26.633165829145728
66	28.650414677054535	21.311887408896705	23.171651168635336	26.86604674541342
67	26.540880503144653	24.67924528301887	22.41509433962264	26.364779874213834
68	27.571392469042205	22.66868840030326	22.896133434420015	26.863785696234523
69	30.225330225330225	20.745920745920746	22.274022274022272	26.754726754726754
70	27.704194260485654	23.758278145695364	23.81346578366446	24.724061810154527
71	36.23584537290121	38.77391643889106	24.99023818820773	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	2.5
2	1.5
3	3.0
4	2.5
5	1.0
6	0.0
7	0.0
8	0.5
9	1.5
10	2.0
11	3.0
12	3.0
13	2.0
14	2.0
15	1.5
16	1.0
17	1.0
18	0.5
19	0.5
20	0.5
21	0.5
22	1.5
23	2.5
24	3.0
25	1.5
26	1.0
27	2.0
28	2.0
29	3.5
30	4.5
31	4.0
32	6.0
33	9.5
34	12.5
35	19.5
36	22.0
37	22.0
38	31.0
39	48.0
40	71.5
41	76.5
42	112.0
43	135.5
44	137.5
45	176.0
46	187.5
47	173.0
48	161.0
49	198.0
50	221.0
51	222.0
52	226.5
53	212.5
54	214.5
55	214.5
56	259.0
57	299.5
58	289.0
59	302.0
60	279.0
61	201.0
62	154.5
63	152.0
64	137.5
65	93.0
66	62.0
67	51.0
68	43.0
69	35.0
70	24.0
71	18.5
72	23.0
73	29.0
74	36.0
75	39.0
76	32.5
77	22.5
78	15.0
79	10.0
80	6.5
81	5.5
82	6.5
83	6.0
84	4.0
85	1.5
86	0.0
87	0.5
88	0.5
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.025
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.025006251562890724
42	0.05001250312578145
43	0.05001250312578145
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.025150905432595575
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40	1.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	1.0
49	0.0
50	0.0
51	1.0
52	1.0
53	1.0
54	1.0
55	2.0
56	0.0
57	1.0
58	1.0
59	1.0
60	3.0
61	3.0
62	1.0
63	2.0
64	0.0
65	1.0
66	3.0
67	19.0
68	96.0
69	237.0
70	1063.0
71	2561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.64999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66488956587966	52.300000000000004
2	10.243716679360244	13.450000000000001
3	3.9223153084539226	7.725
4	1.78979436405179	4.7
5	1.3328255902513328	4.375
6	0.913937547600914	3.5999999999999996
7	0.456968773800457	2.1
8	0.3427265803503427	1.7999999999999998
9	0.456968773800457	2.7
>10	0.8758568164508759	7.249999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCA	22	0.5499999999999999	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCA	19	0.475	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	18	0.44999999999999996	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT	16	0.4	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG	15	0.375	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT	13	0.325	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCA	13	0.325	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGG	13	0.325	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTT	12	0.3	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTT	12	0.3	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGA	12	0.3	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG	12	0.3	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGAC	11	0.27499999999999997	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGA	11	0.27499999999999997	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCC	11	0.27499999999999997	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT	10	0.25	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTT	10	0.25	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	10	0.25	No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGAT	10	0.25	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG	10	0.25	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA	10	0.25	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT	10	0.25	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACC	10	0.25	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACA	9	0.22499999999999998	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGC	9	0.22499999999999998	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG	9	0.22499999999999998	No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA	9	0.22499999999999998	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAA	9	0.22499999999999998	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAA	9	0.22499999999999998	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGT	9	0.22499999999999998	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC	9	0.22499999999999998	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCA	9	0.22499999999999998	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGG	9	0.22499999999999998	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAG	9	0.22499999999999998	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAG	9	0.22499999999999998	No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGG	8	0.2	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC	8	0.2	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAA	8	0.2	No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTT	8	0.2	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT	8	0.2	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAAT	8	0.2	No Hit
CGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAA	8	0.2	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGA	8	0.2	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT	8	0.2	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGG	7	0.17500000000000002	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGG	7	0.17500000000000002	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	7	0.17500000000000002	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT	7	0.17500000000000002	No Hit
GTAGTATACGGCCGTGTCGTCAGATCTCAGGCTGGTTAGGTTCATGTAGCCTGTTGTCAGAGATTCGTCCA	7	0.17500000000000002	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTG	7	0.17500000000000002	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT	7	0.17500000000000002	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA	7	0.17500000000000002	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGG	7	0.17500000000000002	No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAG	7	0.17500000000000002	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGG	7	0.17500000000000002	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAA	7	0.17500000000000002	No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGT	6	0.15	No Hit
GTATACGGCCGTGTCGTCAGATCTCAGGCTGGTTAGGTTCATGTAGCCTGTTGTCAGAGATTCGTCCACAG	6	0.15	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTT	6	0.15	No Hit
ATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG	6	0.15	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGT	6	0.15	No Hit
CTACTGTACGGGGCGAAAAATCCCGAATTTTAGGGGTGTAGTAGTGTCTGGCCCTTTTGTCTACTGGGGC	6	0.15	No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAT	6	0.15	No Hit
GGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	6	0.15	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAA	6	0.15	No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	6	0.15	No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG	6	0.15	No Hit
ACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT	6	0.15	No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTA	6	0.15	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA	6	0.15	No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCG	6	0.15	No Hit
CATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT	6	0.15	No Hit
GCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCT	6	0.15	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA	6	0.15	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATT	6	0.15	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACT	6	0.15	No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA	6	0.15	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC	6	0.15	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGC	6	0.15	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	6	0.15	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGG	5	0.125	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAA	5	0.125	No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGA	5	0.125	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT	5	0.125	No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGG	5	0.125	No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAA	5	0.125	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	5	0.125	No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAA	5	0.125	No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG	5	0.125	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAA	5	0.125	No Hit
GGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG	5	0.125	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACT	5	0.125	No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTT	5	0.125	No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAG	5	0.125	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGA	5	0.125	No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAG	5	0.125	No Hit
TCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTT	5	0.125	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTAC	5	0.125	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAA	5	0.125	No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGAC	5	0.125	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT	5	0.125	No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCG	5	0.125	No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGG	5	0.125	No Hit
GTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACG	5	0.125	No Hit
GATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTG	5	0.125	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTG	5	0.125	No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAA	5	0.125	No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA	5	0.125	No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC	5	0.125	No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG	5	0.125	No Hit
TAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC	5	0.125	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGT	5	0.125	No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTTTGCAATTTATTACTGTCAC	5	0.125	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.125	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13371 READS because READLEN < 1
Read 13371 spots for SRR13211489.sra
Written 13371 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
Rejected 13342 READS because READLEN < 1
Read 13342 spots for SRR13211489.sra
Written 13342 spots for SRR13211489.sra
SRR ids: ['SRR13211489.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_twhgejcs
SRR13211489.sra spots: 640445
blocks: [[1, 13342], [13343, 26684], [26685, 40026], [40027, 53368], [53369, 66710], [66711, 80052], [80053, 93394], [93395, 106736], [106737, 120078], [120079, 133420], [133421, 146762], [146763, 160104], [160105, 173446], [173447, 186788], [186789, 200130], [200131, 213472], [213473, 226814], [226815, 240156], [240157, 253498], [253499, 266840], [266841, 280182], [280183, 293524], [293525, 306866], [306867, 320208], [320209, 333550], [333551, 346892], [346893, 360234], [360235, 373576], [373577, 386918], [386919, 400260], [400261, 413602], [413603, 426944], [426945, 440286], [440287, 453628], [453629, 466970], [466971, 480312], [480313, 493654], [493655, 506996], [506997, 520338], [520339, 533680], [533681, 547022], [547023, 560364], [560365, 573706], [573707, 587048], [587049, 600390], [600391, 613732], [613733, 627074], [627075, 640445]]
SRR13211489 file size 98304
SRR13211489 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13211489 SRR13211489_1.fastq
Input file:	SRR13211489_1.fastq
trimmed:	SRR13211489-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 07:10:19 2022 >> started

Fri May  6 07:10:19 2022 >> done (0.539s)
640445 reads processed; of these:
     0 ( 0.00%) short reads filtered out after trimming by size control
     5 ( 0.00%) empty reads filtered out after trimming by size control
640440 (100.00%) reads available; of these:
   293 ( 0.05%) trimmed reads available after processing
640147 (99.95%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 22	     4	  0.00%
 23	     4	  0.00%
 24	     6	  0.00%
 25	     3	  0.00%
 26	     2	  0.00%
 27	     3	  0.00%
 28	     6	  0.00%
 29	    11	  0.00%
 30	    13	  0.00%
 31	    34	  0.01%
 32	    30	  0.00%
 33	    25	  0.00%
 34	    59	  0.01%
 35	    40	  0.01%
 36	    44	  0.01%
 37	    83	  0.01%
 38	    98	  0.02%
 39	   136	  0.02%
 40	   115	  0.02%
 41	   103	  0.02%
 42	   119	  0.02%
 43	   123	  0.02%
 44	   168	  0.03%
 45	   156	  0.02%
 46	   119	  0.02%
 47	   141	  0.02%
 48	   177	  0.03%
 49	   179	  0.03%
 50	   192	  0.03%
 51	   231	  0.04%
 52	   212	  0.03%
 53	   208	  0.03%
 54	   208	  0.03%
 55	   233	  0.04%
 56	   293	  0.05%
 57	   256	  0.04%
 58	   278	  0.04%
 59	   300	  0.05%
 60	   308	  0.05%
 61	   330	  0.05%
 62	   393	  0.06%
 63	   310	  0.05%
 64	   386	  0.06%
 65	   488	  0.08%
 66	   659	  0.10%
 67	  2838	  0.44%
 68	 16258	  2.54%
 69	 38521	  6.01%
 70	169476	 26.46%
 71	406061	 63.40%
640440 reads passed initial QC


criterion=sequence-density
sequence-density=1.51
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=36
prefix-density=1.52
prefix-fanout=1.9
sequence=CATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGG


criterion=fanout-score
sequence-density=0.09
sequence-density-rank=36
fanout-score=33.58
fanout-score-rank=1
prefix-density=3.16
prefix-fanout=1.0
sequence=GTACATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTA
                                 Started job on |	May 06 07:10:47
                             Started mapping on |	May 06 07:10:48
                                    Finished on |	May 06 07:10:58
       Mapping speed, Million of reads per hour |	230.56

                          Number of input reads |	640440
                      Average input read length |	70
                                    UNIQUE READS:
                   Uniquely mapped reads number |	136107
                        Uniquely mapped reads % |	21.25%
                          Average mapped length |	69.49
                       Number of splices: Total |	18918
            Number of splices: Annotated (sjdb) |	17558
                       Number of splices: GT/AG |	18725
                       Number of splices: GC/AG |	38
                       Number of splices: AT/AC |	4
               Number of splices: Non-canonical |	151
                      Mismatch rate per base, % |	1.00%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.18
                        Insertion rate per base |	0.04%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	462902
             % of reads mapped to multiple loci |	72.28%
        Number of reads mapped to too many loci |	3663
             % of reads mapped to too many loci |	0.57%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.89%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	41431	41431	41431
N_multimapping	462902	462902	462902
N_noFeature	7787	75882	66977
N_ambiguous	2397	733	686
UnstrandedReadsAssigned:125923 PositiveStrandReadsAssigned:59492 NegativeStrandReadsAssigned:68444
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13211489 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13211489-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 640,440 reads, 203,864 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 648 rounds

 180870 SRR13211489.ke.tsv
  58302 SRR13211489.se.tsv
 239172 total
==> SRR13211489.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13211489.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13211489 completed mapping pipeline successfully
