Starting /dee2/code/volunteer_pipeline.sh SRR13211703
    current disk space = 1777711184772
    free memory = 339321876 
SRR13211703 SRAfilesize
1b51b32e526406c89555612c9094126f  SRR13211703.sra
SRR13211703.sra file validated
SRR13211703 is single end
SRR13211703 is conventional basespace
SRR13211703 read1 length is 38-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13211703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	165
Sequences flagged as poor quality	0
Sequence length	38-71
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.951515151515153	32.0	32.0	32.0	32.0	32.0
2	31.472727272727273	32.0	32.0	32.0	32.0	32.0
3	31.53939393939394	32.0	32.0	32.0	32.0	32.0
4	31.64848484848485	32.0	32.0	32.0	32.0	32.0
5	31.606060606060606	32.0	32.0	32.0	32.0	32.0
6	35.24848484848485	36.0	36.0	36.0	36.0	36.0
7	34.8969696969697	36.0	36.0	36.0	36.0	36.0
8	35.054545454545455	36.0	36.0	36.0	36.0	36.0
9	35.39393939393939	36.0	36.0	36.0	36.0	36.0
10	35.29090909090909	36.0	36.0	36.0	36.0	36.0
11	35.43636363636364	36.0	36.0	36.0	36.0	36.0
12	35.14545454545455	36.0	36.0	36.0	36.0	36.0
13	35.06666666666667	36.0	36.0	36.0	36.0	36.0
14	35.0	36.0	36.0	36.0	36.0	36.0
15	35.084848484848486	36.0	36.0	36.0	36.0	36.0
16	34.88484848484848	36.0	36.0	36.0	36.0	36.0
17	34.97575757575758	36.0	36.0	36.0	32.0	36.0
18	35.35757575757576	36.0	36.0	36.0	36.0	36.0
19	35.16969696969697	36.0	36.0	36.0	32.0	36.0
20	35.69090909090909	36.0	36.0	36.0	36.0	36.0
21	35.375757575757575	36.0	36.0	36.0	36.0	36.0
22	35.157575757575756	36.0	36.0	36.0	36.0	36.0
23	35.624242424242425	36.0	36.0	36.0	36.0	36.0
24	35.18787878787879	36.0	36.0	36.0	36.0	36.0
25	35.67272727272727	36.0	36.0	36.0	36.0	36.0
26	35.67878787878788	36.0	36.0	36.0	36.0	36.0
27	35.11515151515152	36.0	36.0	36.0	36.0	36.0
28	35.46666666666667	36.0	36.0	36.0	36.0	36.0
29	34.90909090909091	36.0	36.0	36.0	32.0	36.0
30	35.27878787878788	36.0	36.0	36.0	36.0	36.0
31	34.95151515151515	36.0	36.0	36.0	32.0	36.0
32	34.945454545454545	36.0	36.0	36.0	32.0	36.0
33	34.836363636363636	36.0	36.0	36.0	32.0	36.0
34	34.75757575757576	36.0	36.0	36.0	36.0	36.0
35	34.666666666666664	36.0	36.0	36.0	36.0	36.0
36	34.41818181818182	36.0	36.0	36.0	32.0	36.0
37	35.04848484848485	36.0	36.0	36.0	36.0	36.0
38	33.99393939393939	36.0	36.0	36.0	32.0	36.0
39	34.34756097560975	36.0	36.0	36.0	32.0	36.0
40	34.420731707317074	36.0	36.0	36.0	32.0	36.0
41	34.19512195121951	36.0	36.0	36.0	32.0	36.0
42	33.56707317073171	36.0	36.0	36.0	21.0	36.0
43	34.36585365853659	36.0	36.0	36.0	32.0	36.0
44	34.01829268292683	36.0	36.0	36.0	27.0	36.0
45	34.957317073170735	36.0	36.0	36.0	36.0	36.0
46	34.4601226993865	36.0	36.0	36.0	32.0	36.0
47	34.22699386503067	36.0	36.0	36.0	32.0	36.0
48	34.49079754601227	36.0	36.0	36.0	32.0	36.0
49	34.668711656441715	36.0	36.0	36.0	32.0	36.0
50	34.19631901840491	36.0	36.0	36.0	32.0	36.0
51	34.877300613496935	36.0	36.0	36.0	36.0	36.0
52	33.50617283950617	36.0	36.0	36.0	21.0	36.0
53	33.78395061728395	36.0	36.0	36.0	21.0	36.0
54	33.666666666666664	36.0	36.0	36.0	21.0	36.0
55	33.882716049382715	36.0	36.0	36.0	27.0	36.0
56	33.592592592592595	36.0	36.0	36.0	21.0	36.0
57	34.098765432098766	36.0	36.0	36.0	32.0	36.0
58	33.44444444444444	36.0	36.0	36.0	21.0	36.0
59	33.15432098765432	36.0	36.0	36.0	14.0	36.0
60	32.074074074074076	36.0	32.0	36.0	14.0	36.0
61	32.71604938271605	36.0	36.0	36.0	14.0	36.0
62	33.111111111111114	36.0	36.0	36.0	14.0	36.0
63	32.67283950617284	36.0	36.0	36.0	14.0	36.0
64	33.25308641975309	36.0	36.0	36.0	14.0	36.0
65	32.67901234567901	36.0	36.0	36.0	14.0	36.0
66	32.7037037037037	36.0	36.0	36.0	14.0	36.0
67	32.623456790123456	36.0	36.0	36.0	14.0	36.0
68	32.2360248447205	36.0	32.0	36.0	14.0	36.0
69	32.45	36.0	36.0	36.0	14.0	36.0
70	31.84516129032258	36.0	32.0	36.0	14.0	36.0
71	31.66412213740458	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	1.0
26	1.0
27	3.0
28	6.0
29	3.0
30	4.0
31	3.0
32	8.0
33	9.0
34	26.0
35	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63636363636363	29.6969696969697	12.727272727272727	13.939393939393941
2	15.151515151515152	26.060606060606062	37.57575757575757	21.21212121212121
3	20.0	40.60606060606061	20.606060606060606	18.787878787878785
4	7.2727272727272725	20.0	38.18181818181819	34.54545454545455
5	10.909090909090908	47.27272727272727	26.666666666666668	15.151515151515152
6	27.27272727272727	39.39393939393939	18.181818181818183	15.151515151515152
7	20.0	38.78787878787879	24.242424242424242	16.969696969696972
8	26.666666666666668	30.909090909090907	23.03030303030303	19.393939393939394
9	26.666666666666668	21.818181818181817	25.454545454545453	26.060606060606062
10	14.545454545454545	36.96969696969697	30.303030303030305	18.181818181818183
11	26.666666666666668	27.27272727272727	32.121212121212125	13.939393939393941
12	21.818181818181817	33.33333333333333	29.09090909090909	15.757575757575756
13	20.606060606060606	29.09090909090909	30.909090909090907	19.393939393939394
14	16.969696969696972	26.666666666666668	34.54545454545455	21.818181818181817
15	17.575757575757574	29.6969696969697	34.54545454545455	18.181818181818183
16	19.393939393939394	32.72727272727273	28.484848484848484	19.393939393939394
17	15.151515151515152	35.15151515151515	29.6969696969697	20.0
18	12.727272727272727	28.484848484848484	42.42424242424242	16.363636363636363
19	10.909090909090908	29.09090909090909	40.60606060606061	19.393939393939394
20	13.939393939393941	28.484848484848484	39.39393939393939	18.181818181818183
21	20.0	34.54545454545455	35.75757575757576	9.696969696969697
22	16.969696969696972	26.060606060606062	42.42424242424242	14.545454545454545
23	15.151515151515152	29.6969696969697	43.63636363636363	11.515151515151516
24	18.181818181818183	27.878787878787882	38.18181818181819	15.757575757575756
25	20.0	24.848484848484848	40.0	15.151515151515152
26	12.727272727272727	28.484848484848484	44.24242424242424	14.545454545454545
27	16.969696969696972	30.909090909090907	35.75757575757576	16.363636363636363
28	13.939393939393941	27.27272727272727	40.60606060606061	18.181818181818183
29	15.757575757575756	27.27272727272727	40.0	16.969696969696972
30	8.484848484848486	27.878787878787882	50.303030303030305	13.333333333333334
31	13.939393939393941	31.515151515151512	40.0	14.545454545454545
32	15.757575757575756	26.666666666666668	45.45454545454545	12.121212121212121
33	13.333333333333334	24.848484848484848	47.878787878787875	13.939393939393941
34	19.393939393939394	26.060606060606062	41.81818181818181	12.727272727272727
35	16.969696969696972	26.666666666666668	42.42424242424242	13.939393939393941
36	15.151515151515152	28.484848484848484	45.45454545454545	10.909090909090908
37	13.333333333333334	30.909090909090907	43.63636363636363	12.121212121212121
38	16.969696969696972	26.666666666666668	41.21212121212121	15.151515151515152
39	12.804878048780488	29.878048780487802	43.90243902439025	13.414634146341465
40	14.634146341463413	33.53658536585366	36.58536585365854	15.24390243902439
41	13.414634146341465	29.268292682926827	43.292682926829265	14.02439024390244
42	12.804878048780488	32.926829268292686	43.90243902439025	10.365853658536585
43	14.02439024390244	28.04878048780488	44.51219512195122	13.414634146341465
44	14.02439024390244	34.756097560975604	41.46341463414634	9.75609756097561
45	16.463414634146343	31.097560975609756	44.51219512195122	7.926829268292683
46	17.177914110429448	22.085889570552148	44.785276073619634	15.950920245398773
47	13.496932515337424	30.061349693251532	41.104294478527606	15.337423312883436
48	15.337423312883436	31.28834355828221	43.558282208588956	9.815950920245399
49	11.042944785276074	27.607361963190186	44.171779141104295	17.177914110429448
50	14.723926380368098	28.834355828220858	42.331288343558285	14.11042944785276
51	12.883435582822086	29.447852760736197	44.171779141104295	13.496932515337424
52	11.11111111111111	29.01234567901235	42.592592592592595	17.28395061728395
53	14.19753086419753	29.01234567901235	44.44444444444444	12.345679012345679
54	17.901234567901234	25.308641975308642	43.82716049382716	12.962962962962962
55	16.666666666666664	25.925925925925924	43.20987654320987	14.19753086419753
56	8.641975308641975	32.71604938271605	40.123456790123456	18.51851851851852
57	18.51851851851852	27.160493827160494	41.9753086419753	12.345679012345679
58	12.962962962962962	32.71604938271605	41.9753086419753	12.345679012345679
59	13.580246913580247	29.01234567901235	41.358024691358025	16.049382716049383
60	16.049382716049383	26.543209876543212	38.88888888888889	18.51851851851852
61	16.049382716049383	24.691358024691358	43.82716049382716	15.432098765432098
62	16.049382716049383	25.925925925925924	43.82716049382716	14.19753086419753
63	17.901234567901234	27.160493827160494	43.82716049382716	11.11111111111111
64	12.345679012345679	32.098765432098766	38.2716049382716	17.28395061728395
65	17.28395061728395	27.160493827160494	41.358024691358025	14.19753086419753
66	19.753086419753085	28.39506172839506	40.123456790123456	11.728395061728394
67	14.19753086419753	25.925925925925924	44.44444444444444	15.432098765432098
68	14.906832298136646	28.57142857142857	42.857142857142854	13.664596273291925
69	14.374999999999998	26.25	43.125	16.25
70	18.70967741935484	30.967741935483872	38.064516129032256	12.258064516129032
71	17.557251908396946	37.404580152671755	45.038167938931295	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	7.0
2	5.0
3	7.0
4	5.5
5	2.0
6	0.0
7	0.0
8	1.0
9	6.0
10	10.0
11	9.5
12	6.0
13	2.5
14	2.0
15	2.0
16	1.0
17	0.0
18	1.0
19	1.5
20	0.5
21	0.5
22	1.0
23	1.5
24	2.5
25	1.5
26	2.5
27	4.5
28	3.5
29	2.0
30	2.0
31	3.0
32	1.5
33	2.0
34	4.5
35	4.0
36	3.5
37	3.0
38	3.0
39	6.0
40	7.0
41	4.0
42	4.5
43	7.5
44	6.5
45	5.5
46	6.0
47	5.5
48	4.5
49	4.0
50	6.0
51	6.0
52	2.5
53	1.5
54	2.5
55	3.0
56	2.0
57	3.0
58	3.5
59	3.0
60	4.0
61	3.5
62	2.5
63	1.5
64	0.5
65	0.5
66	1.0
67	0.5
68	0.5
69	1.0
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.5
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
38	1.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	1.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	1.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	1.0
68	1.0
69	5.0
70	24.0
71	131.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.81818181818183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.33333333333333	76.36363636363637
2	2.2222222222222223	3.6363636363636362
3	2.2222222222222223	5.454545454545454
4	0.0	0.0
5	0.0	0.0
6	0.7407407407407408	3.6363636363636362
7	0.0	0.0
8	0.7407407407407408	4.848484848484849
9	0.0	0.0
>10	0.7407407407407408	6.0606060606060606
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	6.0606060606060606	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	4.848484848484849	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	3.6363636363636362	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	1.8181818181818181	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	1.8181818181818181	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	1.8181818181818181	No Hit
GTATATGGGACTGCAGTTTATTTTGAAGTTAGGTCGGTTCTGCTGTGATTTGTCTTTGGTGGAAGTGGTAG	2	1.2121212121212122	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	1.2121212121212122	No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG	2	1.2121212121212122	No Hit
GTATCAACGCAGAGTACATGGGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
GCAAAAAAGATGCTACTCTCAGCGGGTGCTGCTTTAAGGAGGACCTACATCTATTTGCTGACTTTGCCAAG	1	0.6060606060606061	No Hit
ATATTCACCAGTGCTTCTTTTAAATAGTTTCTGACACCTGTTGGAGGCAGGCAGTCCTTCCAATCAAAATA	1	0.6060606060606061	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG	1	0.6060606060606061	No Hit
CTTCTCAGCCTCCGGCATCCGACCAAAACGGATAGCGTTGTGTGACATGCCCAGTGCCAGGCACTTCTGGA	1	0.6060606060606061	No Hit
CTGCCAAAATATGGTGTGAAGGTTGGCCTGACAAATTATGCTGCAGCATATTGTACTGGCCTGCTGCTGG	1	0.6060606060606061	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAG	1	0.6060606060606061	No Hit
GTCTCCAGTATATCTTTATCAGCAGTGTGAAAAGGAACTAATACAAGGAGCGAGGTGTTTCATAGAAATTA	1	0.6060606060606061	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTGGCTATCTAT	1	0.6060606060606061	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCA	1	0.6060606060606061	No Hit
ACTCCGCTCCATTCCAATCCATTCCACTCTTCTCCACTCCAATCGTCTCCGTTCCATTCCATTAAATTCCG	1	0.6060606060606061	No Hit
TATTGGGAGAGAGCACCTCCGTGAAGTCCCGTTTGTGATATTCATCTCGTATGAAGTCTGTTCCGGTATTA	1	0.6060606060606061	No Hit
GGTATCAACGCAGAGTACATGGGCTAACGACAAAAAACAACATCATATTTAAATATTACTTAAATCCTGGA	1	0.6060606060606061	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1	0.6060606060606061	No Hit
ATTATATACCTGTCCCATATATAGCTTCATTGGTATAGGCAAATAAAATGGGTTCAAAAAACTGGAATATA	1	0.6060606060606061	No Hit
GTTTTATCATATATAGAAGAAATATAATTTTTAACTGTCCCTTCTGAAATATATAATTCATCTGCAAT	1	0.6060606060606061	No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTT	1	0.6060606060606061	No Hit
ACATGGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
GTGATTATACTTTCCATATATTAACTCATTTAATCCATACAACAACCTCATGAGGAAAATACTACTATTAT	1	0.6060606060606061	No Hit
AGAATGGACTGGAACGGAATGGTGTCGAATGGAATGGACAGGAAAGCAATGGACCTGAATGAAATGGACT	1	0.6060606060606061	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATTCTGGCCA	1	0.6060606060606061	No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG	1	0.6060606060606061	No Hit
AGGCAGGAGAATCACCTGAACCTGGGAGATGGAGGTTTCAGTGAGCCAAGATCACACCACTGCACTCCAG	1	0.6060606060606061	No Hit
GGCAGAGCCTAGTCAAGCATTACATCACCTGAGTGATCAGTGCAGAGATATGTCACAAAGCCCCCATACG	1	0.6060606060606061	No Hit
CGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCCGGCGGCGTGCG	1	0.6060606060606061	No Hit
GGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	1	0.6060606060606061	No Hit
GAGCTGGAATGAAGGAGCCAGCGTGCAAATAATGAAGGAGCACGGGGGCACCTTCAGTAGCACCGGAATCA	1	0.6060606060606061	No Hit
CGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGATTCCGAGGCGCGGCTCAACGAAGCAGC	1	0.6060606060606061	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	1	0.6060606060606061	No Hit
GTACATGGGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
TAATATATCTCACTGAATCCTGACAATAGCCCTGAGGTAGGTATTACTGTCCCCATTTTGTGTATGAAAGA	1	0.6060606060606061	No Hit
ACGTTGCTACATAATCATTGACTAAAGCCCACAGCTCATTTGGTTTGTTCTCGGGTGGCCGTTTTGTGGG	1	0.6060606060606061	No Hit
CTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTC	1	0.6060606060606061	No Hit
GCTTAATAGCTGGCCCATACAAGACACTATATTTCTTGAATAAATGGACATCAGTTGAATCTAATGTCCA	1	0.6060606060606061	No Hit
GTACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.6060606060606061	No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT	1	0.6060606060606061	No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCA	1	0.6060606060606061	No Hit
GCACAATGGACAGAGATAAAGACAGTGACCTGAAACTCAGTCTACTGAGAAGAGAACAGTATGGGGTGGTG	1	0.6060606060606061	No Hit
ATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTA	1	0.6060606060606061	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAA	1	0.6060606060606061	No Hit
ATACAATGATAATGAAAACAAATTATTTGTCTCCAGAAACCATTTGTTGAAGCTGATTTTGGATAATGAAA	1	0.6060606060606061	No Hit
CCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCC	1	0.6060606060606061	No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAAGTCCTAAACTACCAAAC	1	0.6060606060606061	No Hit
CCCTTACACCTCACCTCGTTAGAACCAAGCTCGACGGAAGCCCTGGTGAGCTTTTAAAACTGAAAGTTAGG	1	0.6060606060606061	No Hit
TATCAACGCAGAGTACATGGGTTTGTTGTACAGATTATTTCATCACCCAGGTATTAAACCCAGTACCCAA	1	0.6060606060606061	No Hit
ACCGAACTGAGCGTCGAAGAACCGGGAGCGTTGTGGTACAGACATGACCAGACGCTGAGGTTTATATGAA	1	0.6060606060606061	No Hit
ACCCAGACTTTGGGAAAAGTCTTTATGTCAGACTATTGTGGGCTAGGGTGACAGATCACACTTTGATAGAT	1	0.6060606060606061	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC	1	0.6060606060606061	No Hit
CAGTAGTGGAGACCAGCAGCTGTGCTTTGTTTTGACAGAGAATGGACTCTCTCTGATTTTGTTTGGTTTGA	1	0.6060606060606061	No Hit
AACAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.6060606060606061	No Hit
CCTTTGAGTATCATTTATGTCAACTTTGTCTCATGTGTATTCCATAAGCTTAAAAATGATATTCAAAGAAA	1	0.6060606060606061	No Hit
TCCTTCAACTTTATTATTGAGGAAGCCAACATAAATCAACACGATTCTTTTCCCACAAAAGTTCCTTTCCA	1	0.6060606060606061	No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGACGTTGGTGTTGTATTCTATAG	1	0.6060606060606061	No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG	1	0.6060606060606061	No Hit
GGCTCAACATGCTACTAACTACCAGCTCTCTGAAGTGTCACTGGGAACAATACACACTCCATGCGTTTTG	1	0.6060606060606061	No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	1	0.6060606060606061	No Hit
CACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCG	1	0.6060606060606061	No Hit
GTACATGGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
GGTGGGGTGATGTAAGGATAATGATTTTAAGTTAGCAGTAAGGTGGGTATGGCTGAAAAGAGGATGTGTGA	1	0.6060606060606061	No Hit
CTCCAGAAGGCACCACCACAGCTGCCCACTTCTGGAAGGTTCCATCTCCTGCTGGCCTGGTCTCCACAAG	1	0.6060606060606061	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTAAACAGGGTTTTTTAAAAAAAACCCCCCCCCC	1	0.6060606060606061	No Hit
GTTACAGACATAATGGATCTGAGATTATGGGAAACAGAAGGTTAATGGTTTAGTAAGCAATCTGTGATTAT	1	0.6060606060606061	No Hit
ATACACAGTAGATTGAATCTAAATTTGAATACTGTCAAAGCATTTCAAAGTACTTATATCTTACCCTTCAA	1	0.6060606060606061	No Hit
ACTCAACCTTCTCTGGGGCTTCCCTGCTTTCTTGAAAGACAAGAAATGTGGAAGAGAAAGATGCTGGACAA	1	0.6060606060606061	No Hit
TTATTGTATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGG	1	0.6060606060606061	No Hit
GAGTACATGGGGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
TAGCTACTCTCATAACCCTCAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTT	1	0.6060606060606061	No Hit
CACCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACA	1	0.6060606060606061	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAA	1	0.6060606060606061	No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATG	1	0.6060606060606061	No Hit
GCTAACTGGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGATGGACAAGTGGCGTTCAGC	1	0.6060606060606061	No Hit
CTCAGGCTCCGGCCACAGCTTAACTGGGCCAAGGTAAAGCTCAGGCTGCCACCCTAGAGGGAGCAAGCCGT	1	0.6060606060606061	No Hit
ATCGAATGGACTCGAATGGAATAACCATTAAACGGAATCGAATGGAATCATTATCGGATGGAAACGAATGG	1	0.6060606060606061	No Hit
ATGACACCCTACTCTTCATTTCACTTTACCTGCTCCTTTTCTTCATTGCACTTATCAACATTTAATATGAT	1	0.6060606060606061	No Hit
GAATCAGGGTTCGATTCCGGAGTGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA	1	0.6060606060606061	No Hit
TATTAGAGCATTAAGGATTTTCTGTGTCAAAGAGTCTAAATTTTATCCTGAAGGAAAATGGGAAACAAAGT	1	0.6060606060606061	No Hit
ACATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGTAAGGGGGGTGCCGGTGGGGGGGGGTTG	1	0.6060606060606061	No Hit
AAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.6060606060606061	No Hit
CTGCTTACCATGTACTCTTCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTAAAAT	1	0.6060606060606061	No Hit
ACATGGGGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
ATGCTACAAAGATCTGGGTTGGTCTGTGACTACTGGAA	1	0.6060606060606061	No Hit
TTCCAGAGGGATCCACTAAGAGGCTAAGAGTTTTATTAGTAAGGCTTTTTTTTTTTTTTTTTGCGTTGCGG	1	0.6060606060606061	No Hit
ATGTGCTTTGTTAAACAGATGCTTGAAGGCAGCATGCTCGTTAAGAGTCATCACCACTCCCTAATCTCAAG	1	0.6060606060606061	No Hit
TCACACAAGTACATCCTATTATACCACCCTCCACCTTGGCAGATGGCTTACCTTGAGTGAAACCATTAA	1	0.6060606060606061	No Hit
GAGCAAAATGTTGGCTAGGGGTAGTGGTGGAAAGTTCCTGATGCCAGATTTTTGAGGTGAGAGAAAGAAAT	1	0.6060606060606061	No Hit
ACATGGGGTACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
GTACATGGGTGGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAGAAAAAAGGAAAAAAAAAAAAA	1	0.6060606060606061	No Hit
GTACATGGGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
GGTACCTGTATAGCTTAGTCATCTAGGTGTGTCTTGTTGCTATAACAAATTACCACAACCTAGTGGCTTAA	1	0.6060606060606061	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTA	1	0.6060606060606061	No Hit
GTACATGGGAAGCAGTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGTTTTTTTAAA	1	0.6060606060606061	No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCG	1	0.6060606060606061	No Hit
GTATACAAGGAGAGTGATAGAAAAATACATAGACTATATAGGCATTAGTCTAAAATTTATAAAATTCAAAA	1	0.6060606060606061	No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGT	1	0.6060606060606061	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGCGGGGGGGGGGG	1	0.6060606060606061	No Hit
AGTTGATTCCATTCCATTCCTTCGATTCCATTCCATTCCATTCCATTCCATTCCATTCCAATCCGGTTGAT	1	0.6060606060606061	No Hit
TGGGATAAGCAGTGGTATCAACGCAGAGTACATGGGATAAGCAGTGGTATCAACGCAGAGTACATGGGATA	1	0.6060606060606061	No Hit
TTCCTTCTCTGATGAGGCAAAAAAAGAATCAAGACTCCTGTTCAAGTAAAGGGCAGAGGGTGAGAGCTAGT	1	0.6060606060606061	No Hit
ACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACAATAAAAAAGCG	1	0.6060606060606061	No Hit
CTACTGGCAGGATCAACCAGGTACCCATGTACTCTGCGTTGATAC	1	0.6060606060606061	No Hit
ATATTGAGCTTCCTTTAGAATTCAGAATATTTCTGTACATCTTGTCATGCTTCAGAATAACATAAAATGGG	1	0.6060606060606061	No Hit
ACATGGATCTAAGTGCACAAACGTAAAAAACATTTTACGATTTTATCGTAATATCCCTATTTGTAATCTTT	1	0.6060606060606061	No Hit
GAGTACTACTTTTTTTTTACTTTTTTTTTACTTTTTTTTTTTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
GTATATAATATGTAGATTGAGTACTTTGATATTTTATGTACAATTTATTATAAATGTTTGGTTACTTTCAT	1	0.6060606060606061	No Hit
CCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCACCTTTCGATGGTAGTCGCCGTGCCTACCAT	1	0.6060606060606061	No Hit
TATCAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
CATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAA	1	0.6060606060606061	No Hit
ACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATA	1	0.6060606060606061	No Hit
TCCCACCACCTCAGTCCCTGAGTAGCTGGGATTACAGGCATGTCGCCCTGCCTGGCTACTTTTTTATTTTT	1	0.6060606060606061	No Hit
GGGACACAGAATCTGGAGATGGTCAGAGTTTGGGAAAGGTGAAAGGTGGGAGGTGGTGTGTGTAAAATGGG	1	0.6060606060606061	No Hit
GTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAA	1	0.6060606060606061	No Hit
GTGTAGGTAGGCCGTCGTCCTAAGTGGCCTCGTGAACTAACGCCTGCTGCTATGTCCCAATGAAGGGTACA	1	0.6060606060606061	No Hit
CGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAG	1	0.6060606060606061	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCA	1	0.6060606060606061	No Hit
TATAAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTATTGTTTTTTATTT	1	0.6060606060606061	No Hit
TCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA	1	0.6060606060606061	No Hit
GAAGCAACATTTATACTGAGCCTGAAGAATGGCAAAGGAAGAGAGGAAACATGATAGAGGCAGAGAGTTCA	1	0.6060606060606061	No Hit
GACTTAGTTACAGACATAATGGATCTGAGATTATGGGAAACAGAAGGTTAATGGTTTAGTAAGCAAT	1	0.6060606060606061	No Hit
AGTTTAATTAGCTCCCACAAATGTGTGAGAATATGCAAAGTTTGTTTTTCAATGCCTGGTTTCTTTCACTT	1	0.6060606060606061	No Hit
ATCGCACGCCCCCCGTGGCGGCGACGCCCCATTCGAACGTCTGCCCTATCAACTTCCGATGGTAGTCGCCG	1	0.6060606060606061	No Hit
CCTTATAATTTTGGTACTAGAAGAAACAGAAGTACAACTACTATGACATAATAACCTTCATATGATTATTT	1	0.6060606060606061	No Hit
ACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.6060606060606061	No Hit
AGGTTGTCTAGTTCAGTGGGCATGTTAGTTGTTTCCAGACAGTCTTCTTTGGCGGCAATGATGTGGCTGTT	1	0.6060606060606061	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.6060606060606061	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 3 READS because READLEN < 1
Read 3 spots for SRR13211703.sra
Written 3 spots for SRR13211703.sra
Rejected 24 READS because READLEN < 1
Read 24 spots for SRR13211703.sra
Written 24 spots for SRR13211703.sra
SRR ids: ['SRR13211703.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_1jty_b1g
SRR13211703.sra spots: 165
blocks: [[1, 3], [4, 6], [7, 9], [10, 12], [13, 15], [16, 18], [19, 21], [22, 24], [25, 27], [28, 30], [31, 33], [34, 36], [37, 39], [40, 42], [43, 45], [46, 48], [49, 51], [52, 54], [55, 57], [58, 60], [61, 63], [64, 66], [67, 69], [70, 72], [73, 75], [76, 78], [79, 81], [82, 84], [85, 87], [88, 90], [91, 93], [94, 96], [97, 99], [100, 102], [103, 105], [106, 108], [109, 111], [112, 114], [115, 117], [118, 120], [121, 123], [124, 126], [127, 129], [130, 132], [133, 135], [136, 138], [139, 141], [142, 165]]
SRR13211703 file size 1
SRR13211703 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13211703 SRR13211703_1.fastq
Input file:	SRR13211703_1.fastq
trimmed:	SRR13211703-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 03:55:59 2022 >> started

Fri May  6 03:55:59 2022 >> done (0.005s)
165 reads processed; of these:
  0 ( 0.00%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
165 (100.00%) reads available; of these:
165 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	  1	  0.61%
 39	  0	  0.00%
 40	  0	  0.00%
 41	  0	  0.00%
 42	  0	  0.00%
 43	  0	  0.00%
 44	  0	  0.00%
 45	  1	  0.61%
 46	  0	  0.00%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  1	  0.61%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  1	  0.61%
 68	  1	  0.61%
 69	  5	  3.03%
 70	 24	 14.55%
 71	131	 79.39%
165 reads passed initial QC
                                 Started job on |	May 06 03:56:17
                             Started mapping on |	May 06 03:56:18
                                    Finished on |	May 06 03:56:21
       Mapping speed, Million of reads per hour |	0.20

                          Number of input reads |	165
                      Average input read length |	70
                                    UNIQUE READS:
                   Uniquely mapped reads number |	71
                        Uniquely mapped reads % |	43.03%
                          Average mapped length |	67.92
                       Number of splices: Total |	7
            Number of splices: Annotated (sjdb) |	3
                       Number of splices: GT/AG |	7
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	1.08%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	52
             % of reads mapped to multiple loci |	31.52%
        Number of reads mapped to too many loci |	18
             % of reads mapped to too many loci |	10.91%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.94%
                     % of reads unmapped: other |	0.61%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	42	42	42
N_multimapping	52	52	52
N_noFeature	50	61	60
N_ambiguous	0	0	0
UnstrandedReadsAssigned:21 PositiveStrandReadsAssigned:10 NegativeStrandReadsAssigned:11
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13211703 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13211703-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 165 reads, 73 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 256 rounds

 180870 SRR13211703.ke.tsv
  58302 SRR13211703.se.tsv
 239172 total
==> SRR13211703.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13211703.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13211703 completed mapping pipeline successfully
