Starting /dee2/code/volunteer_pipeline.sh SRR13211411
    current disk space = 1770599893788
    free memory = 341658548 
SRR13211411 SRAfilesize
28eb4c59dcc13e474acc57f539357ea4  SRR13211411.sra
SRR13211411.sra file validated
SRR13211411 is paired end
SRR13211411 is conventional basespace
SRR13211411 read1 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13211411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.069	32.0	32.0	32.0	32.0	32.0
2	31.242	32.0	32.0	32.0	32.0	32.0
3	31.26575	32.0	32.0	32.0	32.0	32.0
4	31.36825	32.0	32.0	32.0	32.0	32.0
5	31.4125	32.0	32.0	32.0	32.0	32.0
6	34.9335	36.0	36.0	36.0	36.0	36.0
7	34.93425	36.0	36.0	36.0	36.0	36.0
8	34.955	36.0	36.0	36.0	36.0	36.0
9	35.11375	36.0	36.0	36.0	36.0	36.0
10	34.963	36.0	36.0	36.0	36.0	36.0
11	35.02275	36.0	36.0	36.0	36.0	36.0
12	35.038	36.0	36.0	36.0	36.0	36.0
13	35.01	36.0	36.0	36.0	36.0	36.0
14	35.02775	36.0	36.0	36.0	36.0	36.0
15	34.9935	36.0	36.0	36.0	36.0	36.0
16	34.98275	36.0	36.0	36.0	36.0	36.0
17	34.969	36.0	36.0	36.0	36.0	36.0
18	35.01725	36.0	36.0	36.0	36.0	36.0
19	34.8805	36.0	36.0	36.0	32.0	36.0
20	34.69575	36.0	36.0	36.0	32.0	36.0
21	34.89975	36.0	36.0	36.0	32.0	36.0
22	34.857	36.0	36.0	36.0	32.0	36.0
23	34.8395	36.0	36.0	36.0	32.0	36.0
24	34.7045	36.0	36.0	36.0	32.0	36.0
25	34.7005	36.0	36.0	36.0	32.0	36.0
26	34.62275	36.0	36.0	36.0	32.0	36.0
27	34.6255	36.0	36.0	36.0	32.0	36.0
28	34.55775	36.0	36.0	36.0	32.0	36.0
29	34.594	36.0	36.0	36.0	32.0	36.0
30	34.56725	36.0	36.0	36.0	32.0	36.0
31	34.74875	36.0	36.0	36.0	32.0	36.0
32	34.5805	36.0	36.0	36.0	32.0	36.0
33	33.7955	36.0	36.0	36.0	32.0	36.0
34	31.91525	36.0	36.0	36.0	14.0	36.0
35	23.74175	36.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	4.0
24	6.0
25	12.0
26	22.0
27	36.0
28	45.0
29	90.0
30	123.0
31	176.0
32	306.0
33	613.0
34	1129.0
35	1436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2	20.575	13.475000000000001	22.75
2	15.675	21.075	36.225	27.025
3	18.6	27.85	30.325000000000003	23.225
4	12.35	18.425	36.75	32.475
5	15.049999999999999	37.2	30.775000000000002	16.975
6	31.221832749123685	34.20130195292939	17.826740110165247	16.750125187781673
7	26.775	34.1	20.75	18.375
8	29.225	30.725	19.400000000000002	20.65
9	28.425	14.475	21.125	35.975
10	18.7	27.125	29.525000000000002	24.65
11	34.1	23.75	21.625	20.525
12	26.625	26.35	27.675	19.35
13	29.099999999999998	20.7	24.775	25.424999999999997
14	25.124999999999996	22.2	24.474999999999998	28.199999999999996
15	28.825	23.175	24.2	23.799999999999997
16	24.2	24.55	28.675	22.575
17	23.325000000000003	25.124999999999996	26.8	24.75
18	24.45	24.525	26.35	24.675
19	27.35	25.0	25.525	22.125
20	26.85	25.4	26.575	21.175
21	26.700000000000003	24.125	26.924999999999997	22.25
22	27.675	24.025	23.7	24.6
23	23.799999999999997	25.5	24.775	25.924999999999997
24	24.575	27.875	24.7	22.85
25	23.755938984746187	24.781195298824706	24.10602650662666	27.35683920980245
26	24.306076519129782	24.056014003500874	27.68192048012003	23.95598899724931
27	23.817863397548162	23.817863397548162	25.64423317488116	26.720040030022517
28	23.20580145036259	25.506376594148538	26.906726681670417	24.381095273818453
29	25.581395348837212	23.25581395348837	27.68192048012003	23.48087021755439
30	24.262131065532767	25.912956478239117	28.039019509754876	21.785892946473236
31	29.54431647471207	23.109664496745115	22.00801201802704	25.338007010515774
32	22.479255720392256	24.94342469197888	25.823485038974102	26.753834548654766
33	24.243201641867625	25.295023088763468	27.96305797845049	22.498717290918417
34	25.572519083969464	26.417666303162484	29.116684841875685	18.893129770992367
35	30.684326710816777	38.373804267844	30.94186902133922	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	5.5
5	11.0
6	11.0
7	8.5
8	6.0
9	6.0
10	5.0
11	4.0
12	4.0
13	5.5
14	7.0
15	7.0
16	12.5
17	18.0
18	18.0
19	24.0
20	30.0
21	41.0
22	52.0
23	52.0
24	54.5
25	57.0
26	57.0
27	68.0
28	79.0
29	79.0
30	86.0
31	93.0
32	93.0
33	136.5
34	180.0
35	180.0
36	190.0
37	200.0
38	200.0
39	259.0
40	318.0
41	335.0
42	352.0
43	352.0
44	394.5
45	437.0
46	437.0
47	486.5
48	536.0
49	536.0
50	476.5
51	417.0
52	417.0
53	341.5
54	266.0
55	266.0
56	242.5
57	219.0
58	219.0
59	209.0
60	199.0
61	170.0
62	141.0
63	141.0
64	122.5
65	104.0
66	104.0
67	100.5
68	97.0
69	97.0
70	84.0
71	71.0
72	71.0
73	60.5
74	50.0
75	50.0
76	39.0
77	28.0
78	28.0
79	22.5
80	17.0
81	11.0
82	5.0
83	5.0
84	4.0
85	3.0
86	3.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.15
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.025
26	0.025
27	0.075
28	0.025
29	0.025
30	0.05
31	0.15
32	0.575
33	2.55
34	8.3
35	32.05
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.38153647514525	70.775
2	4.099418979987089	6.35
3	1.7107811491284701	3.975
4	0.6455777921239509	2.0
5	0.4519044544867657	1.7500000000000002
6	0.48418334409296315	2.25
7	0.12911555842479017	0.7000000000000001
8	0.16139444803098774	1.0
9	0.25823111684958033	1.7999999999999998
>10	0.6455777921239509	7.825
>50	0.03227888960619754	1.575
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	63	1.575	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	45	1.125	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	25	0.625	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	23	0.575	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	20	0.5	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	18	0.44999999999999996	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATT	16	0.4	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTA	16	0.4	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	15	0.375	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAT	15	0.375	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	13	0.325	No Hit
TTCCAGCACCCGGCGGCGGACGCTCTTGGCAGGGT	13	0.325	No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCA	12	0.3	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT	11	0.27499999999999997	No Hit
CTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTA	11	0.27499999999999997	No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAA	10	0.25	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGT	10	0.25	No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACAT	10	0.25	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTA	10	0.25	No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGG	10	0.25	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	10	0.25	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTN	9	0.22499999999999998	No Hit
ACCCTCACGGGCGGCGCCTTCCAGCACCCGGCGGN	9	0.22499999999999998	No Hit
GCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGN	9	0.22499999999999998	No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGANNN	9	0.22499999999999998	No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTT	9	0.22499999999999998	No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG	9	0.22499999999999998	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
TTTCTGGCCATCGCAATGCTATGTTTTAATTAGAN	9	0.22499999999999998	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT	8	0.2	No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGA	8	0.2	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	8	0.2	No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCAT	8	0.2	No Hit
GGATAAGGATTGGCTCTAAGGGTTGGGTGCGTTGG	8	0.2	No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT	7	0.17500000000000002	No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATN	7	0.17500000000000002	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTG	7	0.17500000000000002	No Hit
GGTTGGCACTACCCTCACGGGCGGCGCCTTCCAGN	6	0.15	No Hit
AAATAGATCCGTAACTTCGGGATAAGGATTGGCTN	6	0.15	No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTT	6	0.15	No Hit
GGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGN	6	0.15	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGNN	6	0.15	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCN	6	0.15	No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG	6	0.15	No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT	6	0.15	No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGAT	6	0.15	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	6	0.15	No Hit
ATCACATTGCGTCAACACCATTTTCTGGCCATCGN	6	0.15	No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGN	6	0.15	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGG	6	0.15	No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAA	5	0.125	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCA	5	0.125	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGT	5	0.125	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATG	5	0.125	No Hit
GTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCN	5	0.125	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA	5	0.125	No Hit
ATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTA	5	0.125	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTANN	5	0.125	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGA	5	0.125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAA	5	0.125	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTG	5	0.125	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	5	0.125	No Hit
CTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.05	0.0	0.0	0.0	0.0
17	0.05	0.0	0.0	0.0	0.0
18	0.05	0.0	0.0	0.0	0.0
19	0.05	0.0	0.0	0.0	0.0
20	0.05	0.0	0.0	0.0	0.0
21	0.05	0.0	0.0	0.0	0.0
22	0.05	0.0	0.0	0.0	0.0
23	0.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR13211411 read2 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13211411_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7685	32.0	32.0	32.0	32.0	32.0
2	30.1295	32.0	32.0	32.0	21.0	32.0
3	30.3865	32.0	32.0	32.0	21.0	32.0
4	30.432	32.0	32.0	32.0	32.0	32.0
5	30.1825	32.0	32.0	32.0	21.0	32.0
6	33.8705	36.0	36.0	36.0	32.0	36.0
7	33.735	36.0	36.0	36.0	27.0	36.0
8	33.71325	36.0	36.0	36.0	27.0	36.0
9	34.161	36.0	36.0	36.0	32.0	36.0
10	33.51875	36.0	36.0	36.0	21.0	36.0
11	33.78125	36.0	36.0	36.0	32.0	36.0
12	33.6505	36.0	36.0	36.0	27.0	36.0
13	33.81575	36.0	36.0	36.0	32.0	36.0
14	33.619	36.0	36.0	36.0	27.0	36.0
15	33.9105	36.0	36.0	36.0	32.0	36.0
16	33.52975	36.0	36.0	36.0	21.0	36.0
17	33.84775	36.0	36.0	36.0	32.0	36.0
18	33.86375	36.0	36.0	36.0	32.0	36.0
19	33.62375	36.0	36.0	36.0	27.0	36.0
20	33.58475	36.0	36.0	36.0	27.0	36.0
21	33.79175	36.0	36.0	36.0	32.0	36.0
22	33.75775	36.0	36.0	36.0	32.0	36.0
23	33.56175	36.0	36.0	36.0	21.0	36.0
24	33.515	36.0	36.0	36.0	21.0	36.0
25	33.40775	36.0	36.0	36.0	21.0	36.0
26	33.61	36.0	36.0	36.0	27.0	36.0
27	33.50625	36.0	36.0	36.0	21.0	36.0
28	33.236	36.0	36.0	36.0	21.0	36.0
29	33.51675	36.0	36.0	36.0	21.0	36.0
30	33.35125	36.0	36.0	36.0	21.0	36.0
31	33.493	36.0	36.0	36.0	21.0	36.0
32	33.16575	36.0	36.0	36.0	21.0	36.0
33	32.683	36.0	36.0	36.0	14.0	36.0
34	30.7845	36.0	36.0	36.0	14.0	36.0
35	23.0765	36.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	3.0
17	2.0
18	4.0
19	7.0
20	17.0
21	20.0
22	19.0
23	29.0
24	33.0
25	49.0
26	73.0
27	81.0
28	115.0
29	146.0
30	195.0
31	282.0
32	414.0
33	624.0
34	934.0
35	950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86215538847117	19.248120300751882	13.358395989974936	22.531328320802004
2	15.043804755944931	21.501877346683354	38.698372966207764	24.755944931163956
3	18.65466366591648	29.75743935983996	28.95723930982746	22.630657664416105
4	12.928232058014505	16.27906976744186	36.05901475368842	34.733683420855215
5	14.674999999999999	39.7	29.925	15.7
6	30.049999999999997	35.449999999999996	19.75	14.75
7	28.275	33.45	19.825	18.45
8	30.65	30.375000000000004	18.8	20.175
9	31.624999999999996	14.05	20.9	33.425
10	19.479869967491872	27.631907976994246	28.00700175043761	24.88122030507627
11	33.375	25.124999999999996	21.2	20.3
12	26.85	27.650000000000002	26.6	18.9
13	27.981995498874717	21.530382595648913	25.23130782695674	25.256314078519633
14	23.63090772693173	24.056014003500874	24.956239059764943	27.35683920980245
15	29.657414353588397	23.48087021755439	24.031007751937985	22.83070767691923
16	25.03125781445361	26.506626656664167	26.93173293323331	21.530382595648913
17	24.05	26.174999999999997	25.924999999999997	23.849999999999998
18	24.95	26.25	26.775	22.025
19	26.450000000000003	26.375	26.075	21.099999999999998
20	26.881720430107524	26.831707926981746	23.58089522380595	22.705676419104776
21	26.113056528264135	23.261630815407706	28.214107053526767	22.411205602801402
22	28.46423211605803	22.98649324662331	23.011505752876438	25.53776888444222
23	23.08654327163582	27.138569284642323	24.987493746873437	24.787393696848426
24	24.774774774774773	28.928928928928926	24.24924924924925	22.047047047047048
25	24.568426319739807	25.519139354515886	22.792094070552913	27.120340255191394
26	25.33166458072591	25.03128911138924	25.782227784730914	23.85481852315394
27	23.309964947421133	25.863795693540307	24.261392088132197	26.564847270906363
28	24.18627941912869	26.81522283425138	25.913870806209317	23.084626940410615
29	25.963945918878316	25.062593890836254	25.63845768652979	23.335002503755632
30	24.036054081121684	27.44116174261392	26.389584376564844	22.13319979969955
31	28.596491228070175	23.884711779448622	21.37844611528822	26.14035087719298
32	21.4321608040201	23.894472361809047	26.633165829145728	28.04020100502513
33	24.193548387096776	27.956989247311824	27.21454173067076	20.634920634920633
34	24.591503267973856	26.143790849673206	28.567538126361658	20.697167755991288
35	32.404055032585084	38.595220854453295	29.000724112961624	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	6.0
2	4.0
3	4.0
4	8.5
5	13.0
6	13.0
7	11.5
8	10.0
9	10.0
10	8.0
11	6.0
12	6.0
13	7.5
14	9.0
15	9.0
16	10.5
17	12.0
18	12.0
19	20.0
20	28.0
21	34.5
22	41.0
23	41.0
24	45.0
25	49.0
26	49.0
27	71.5
28	94.0
29	94.0
30	111.5
31	129.0
32	129.0
33	159.0
34	189.0
35	189.0
36	208.0
37	227.0
38	227.0
39	263.0
40	299.0
41	308.0
42	317.0
43	317.0
44	373.0
45	429.0
46	429.0
47	481.0
48	533.0
49	533.0
50	462.5
51	392.0
52	392.0
53	330.0
54	268.0
55	268.0
56	245.5
57	223.0
58	223.0
59	197.0
60	171.0
61	162.0
62	153.0
63	153.0
64	140.0
65	127.0
66	127.0
67	111.0
68	95.0
69	95.0
70	82.0
71	69.0
72	69.0
73	56.0
74	43.0
75	43.0
76	36.0
77	29.0
78	29.0
79	20.0
80	11.0
81	10.5
82	10.0
83	10.0
84	7.0
85	4.0
86	4.0
87	4.0
88	4.0
89	4.0
90	3.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.5
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.25
2	0.125
3	0.025
4	0.025
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.025
11	0.0
12	0.0
13	0.025
14	0.025
15	0.025
16	0.025
17	0.0
18	0.0
19	0.0
20	0.025
21	0.05
22	0.05
23	0.05
24	0.1
25	0.075
26	0.125
27	0.15
28	0.15
29	0.15
30	0.15
31	0.25
32	0.5
33	2.35
34	8.200000000000001
35	30.95
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.10000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.54108723135272	73.2
2	3.4134007585335016	5.4
3	1.359039190897598	3.225
4	0.695322376738306	2.1999999999999997
5	0.6005056890012642	2.375
6	0.31605562579013907	1.5
7	0.28445006321112515	1.575
8	0.18963337547408343	1.2
9	0.12642225031605564	0.8999999999999999
>10	0.4424778761061947	6.175
>50	0.03160556257901391	2.25
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	90	2.25	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	35	0.8750000000000001	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	30	0.75	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	23	0.575	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	21	0.525	No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATT	20	0.5	No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGG	16	0.4	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTA	16	0.4	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA	15	0.375	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTT	14	0.35000000000000003	No Hit
TTCCAGCACCCGGCGGCGGACGCTCTTGGCAGGGT	13	0.325	No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCA	12	0.3	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTG	12	0.3	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	10	0.25	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAT	10	0.25	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	9	0.22499999999999998	No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAA	9	0.22499999999999998	No Hit
CTCTTGGCAGGGTTCGCCCGTCCGGCGCACGCTTA	9	0.22499999999999998	No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCAT	9	0.22499999999999998	No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAA	8	0.2	No Hit
AAATCACATTGCGTCAACACCATTTTCTGGCCATN	8	0.2	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACAT	8	0.2	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTA	8	0.2	No Hit
GGATTGGCTCTAAGGGTTGGGTGCGTTGGGCCTTG	8	0.2	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
GGTTGGCACTACCCTCACGGGCGGCGCCTTCCAGN	7	0.17500000000000002	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTN	7	0.17500000000000002	No Hit
ACCCTCACGGGCGGCGCCTTCCAGCACCCGGCGGN	7	0.17500000000000002	No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACAT	7	0.17500000000000002	No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAG	7	0.17500000000000002	No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACACN	7	0.17500000000000002	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7	0.17500000000000002	No Hit
GGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGN	7	0.17500000000000002	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT	6	0.15	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGNN	6	0.15	No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT	6	0.15	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	6	0.15	No Hit
GCTCTAAGGGTTGGGTGCGTTGGGCCTTGAGCAGA	6	0.15	No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGN	6	0.15	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGT	6	0.15	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.15	No Hit
GGATAAGGATTGGCTCTAAGGGTTGGGTGCGTTGG	6	0.15	No Hit
GTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCN	5	0.125	No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTT	5	0.125	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTG	5	0.125	No Hit
CCTCACGGGCGGCGCCTTCCAGCACCCGGCGGCGG	5	0.125	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCN	5	0.125	No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGA	5	0.125	No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGANNN	5	0.125	No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCN	5	0.125	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA	5	0.125	No Hit
CTTAACAACCAACTTAGAACTGGTACGGACAAGGG	5	0.125	No Hit
TACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGG	5	0.125	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	5	0.125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAA	5	0.125	No Hit
GGGTTGGGTGCGTTGGGCCTTGAGCAGAAGCCNNN	5	0.125	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG	5	0.125	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	5	0.125	No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAG	5	0.125	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCANN	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.025	0.0	0.0	0.0	0.0
13	0.05	0.0	0.0	0.0	0.0
14	0.05	0.0	0.0	0.0	0.0
15	0.05	0.0	0.0	0.0	0.0
16	0.075	0.0	0.0	0.0	0.0
17	0.075	0.0	0.0	0.0	0.0
18	0.075	0.0	0.0	0.0	0.0
19	0.075	0.0	0.0	0.0	0.0
20	0.075	0.0	0.0	0.0	0.0
21	0.075	0.0	0.0	0.0	0.0
22	0.075	0.0	0.0	0.0	0.0
23	0.075	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	65	1.582066E-5	17.66154	1
AGTACAT	50	0.0011716894	17.22	13
AGAGTAC	70	2.9976627E-5	16.400002	11
GAGTACA	55	0.0022206898	15.654545	12
AACGCAG	75	5.4243406E-5	15.306666	6
ACGCAGA	75	5.4243406E-5	15.306666	7
TCAACGC	75	5.4243406E-5	15.306666	4
CAGAGTA	75	5.4243406E-5	15.306666	10
GCAGAGT	75	5.4243406E-5	15.306666	9
CGCAGAG	75	5.4243406E-5	15.306666	8
ATCAACG	75	5.4243406E-5	15.306666	3
CAACGCA	75	5.4243406E-5	15.306666	5
TATCAAC	80	9.42973E-5	14.349999	2
GTACATG	65	0.0067639267	13.246155	14
TACATGG	65	0.0067639267	13.246155	15
>>END_MODULE
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
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Read 24889 spots for SRR13211411.sra
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Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
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Read 24889 spots for SRR13211411.sra
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Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24908 spots for SRR13211411.sra
Written 24908 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
Read 24889 spots for SRR13211411.sra
Written 24889 spots for SRR13211411.sra
SRR ids: ['SRR13211411.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_nzmlwjbg
SRR13211411.sra spots: 1194691
blocks: [[1, 24889], [24890, 49778], [49779, 74667], [74668, 99556], [99557, 124445], [124446, 149334], [149335, 174223], [174224, 199112], [199113, 224001], [224002, 248890], [248891, 273779], [273780, 298668], [298669, 323557], [323558, 348446], [348447, 373335], [373336, 398224], [398225, 423113], [423114, 448002], [448003, 472891], [472892, 497780], [497781, 522669], [522670, 547558], [547559, 572447], [572448, 597336], [597337, 622225], [622226, 647114], [647115, 672003], [672004, 696892], [696893, 721781], [721782, 746670], [746671, 771559], [771560, 796448], [796449, 821337], [821338, 846226], [846227, 871115], [871116, 896004], [896005, 920893], [920894, 945782], [945783, 970671], [970672, 995560], [995561, 1020449], [1020450, 1045338], [1045339, 1070227], [1070228, 1095116], [1095117, 1120005], [1120006, 1144894], [1144895, 1169783], [1169784, 1194691]]
SRR13211411 file size 65536
SRR13211411 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13211411 SRR13211411_1.fastq SRR13211411_2.fastq
Input file:	SRR13211411_1.fastq
Paired file:	SRR13211411_2.fastq
trimmed:	SRR13211411-trimmed-pair1.fastq, SRR13211411-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Fri May  6 08:36:45 2022 >> started

Fri May  6 08:36:46 2022 >> done (1.107s)
1194691 read pairs processed; of these:
      2 ( 0.00%) short read pairs filtered out after trimming by size control
      8 ( 0.00%) empty read pairs filtered out after trimming by size control
1194681 (100.00%) read pairs available; of these:
 639369 (53.52%) trimmed read pairs available after processing
 555312 (46.48%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 21	      4	  0.00%
 22	      6	  0.00%
 23	      7	  0.00%
 24	      4	  0.00%
 25	      7	  0.00%
 26	     10	  0.00%
 27	     21	  0.00%
 28	     37	  0.00%
 29	    108	  0.01%
 30	    422	  0.04%
 31	    821	  0.07%
 32	   6983	  0.58%
 33	  77151	  6.46%
 34	 553788	 46.35%
 35	 555312	 46.48%
1194681 reads passed initial QC


criterion=sequence-density
sequence-density=2.06
sequence-density-rank=1
fanout-score=4.34
fanout-score-rank=40
prefix-density=8.94
prefix-fanout=1.0
sequence=GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTC


criterion=fanout-score
sequence-density=0.15
sequence-density-rank=29
fanout-score=77.33
fanout-score-rank=1
prefix-density=11.48
prefix-fanout=1.0
sequence=ACGCAGAGTACTTTTT


criterion=sequence-density
sequence-density=2.16
sequence-density-rank=1
fanout-score=4.26
fanout-score-rank=41
prefix-density=9.09
prefix-fanout=1.0
sequence=GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTC


criterion=fanout-score
sequence-density=0.14
sequence-density-rank=37
fanout-score=86.53
fanout-score-rank=1
prefix-density=12.00
prefix-fanout=1.0
sequence=ACGCAGAGTACTTTTT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTC -y GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTC -o SRR13211411 SRR13211411_1.fastq SRR13211411_2.fastq
Input file:	SRR13211411_1.fastq
Paired file:	SRR13211411_2.fastq
trimmed:	SRR13211411-trimmed-pair1.fastq, SRR13211411-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT
-- paired 3' end adapter sequence (-y):	GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Fri May  6 08:36:56 2022 >> started

Fri May  6 08:36:56 2022 >> done (0.513s)
398227 read pairs processed; of these:
     1 ( 0.00%) short read pairs filtered out after trimming by size control
  2507 ( 0.63%) empty read pairs filtered out after trimming by size control
395719 (99.37%) read pairs available; of these:
   245 ( 0.06%) trimmed read pairs available after processing
395474 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 21	     1	  0.00%
 22	     2	  0.00%
 23	     1	  0.00%
 24	     1	  0.00%
 25	     2	  0.00%
 26	     3	  0.00%
 27	     8	  0.00%
 28	    13	  0.00%
 29	    29	  0.01%
 30	   142	  0.04%
 31	   291	  0.07%
 32	  2325	  0.59%
 33	 25749	  6.51%
 34	183712	 46.42%
 35	183440	 46.36%


criterion=sequence-density
sequence-density=2.03
sequence-density-rank=1
fanout-score=4.41
fanout-score-rank=39
prefix-density=8.94
prefix-fanout=1.0
sequence=GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTC


criterion=fanout-score
sequence-density=0.15
sequence-density-rank=28
fanout-score=77.26
fanout-score-rank=1
prefix-density=11.50
prefix-fanout=1.0
sequence=ACGCAGAGTACTTTTT


criterion=sequence-density
sequence-density=2.13
sequence-density-rank=1
fanout-score=4.33
fanout-score-rank=41
prefix-density=9.10
prefix-fanout=1.0
sequence=GAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCGCCGGGTGCTGGAAGGCGCCGCCCGTGAGGGTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGGATTTTCAAGGGTCGTCGAGAGCGCACCGGACAGCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTAAGAAGAAAAGAGAACTCTTC


criterion=fanout-score
sequence-density=0.14
sequence-density-rank=37
fanout-score=86.56
fanout-score-rank=1
prefix-density=12.02
prefix-fanout=1.0
sequence=ACGCAGAGTACTTTTT
SRR13211411 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 06 08:37:59
                             Started mapping on |	May 06 08:37:59
                                    Finished on |	May 06 08:38:25
       Mapping speed, Million of reads per hour |	165.07

                          Number of input reads |	1192173
                      Average input read length |	45
                                    UNIQUE READS:
                   Uniquely mapped reads number |	246965
                        Uniquely mapped reads % |	20.72%
                          Average mapped length |	44.49
                       Number of splices: Total |	7861
            Number of splices: Annotated (sjdb) |	7742
                       Number of splices: GT/AG |	7771
                       Number of splices: GC/AG |	68
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	22
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	113442
             % of reads mapped to multiple loci |	9.52%
        Number of reads mapped to too many loci |	4389
             % of reads mapped to too many loci |	0.37%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	68.62%
                     % of reads unmapped: other |	0.78%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	831777	831777	831777
N_multimapping	113442	113442	113442
N_noFeature	153742	198946	199042
N_ambiguous	4436	1012	910
UnstrandedReadsAssigned:88787 PositiveStrandReadsAssigned:47007 NegativeStrandReadsAssigned:47013
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13211411 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 180,869
[index] number of k-mers: 102,188,183
[index] number of equivalence classes: 1,184,234
[quant] running in paired-end mode
[quant] will process pair 1: SRR13211411-trimmed-pair1.fastq
                             SRR13211411-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,192,173 reads, 210,560 reads pseudoaligned
[quant] estimated average fragment length: 108.958
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 935 rounds

 180870 SRR13211411.ke.tsv
  58302 SRR13211411.se.tsv
 239172 total
==> SRR13211411.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	2.66667	0	0
ENST00000448914.1	13	3	0	0
ENST00000415118.1	8	1.66667	0	0
ENST00000631435.1	12	3.66667	0	0
ENST00000632684.1	12	3.66667	0	0
ENST00000454908.1	17	3.78125	0	0
ENST00000390567.1	20	4.33333	0	0
ENST00000603326.1	19	4.11765	0	0
ENST00000414852.1	16	4.04545	0	0

==> SRR13211411.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13211411 completed mapping pipeline successfully
