Starting /dee2/code/volunteer_pipeline.sh SRR13210712
    current disk space = 1769122789724
    free memory = 345684472 
SRR13210712 SRAfilesize
b95d716e869ab971073cc22ece1e2350  SRR13210712.sra
SRR13210712.sra file validated
SRR13210712 is paired end
SRR13210712 is conventional basespace
SRR13210712 read1 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.565	32.0	32.0	32.0	32.0	32.0
2	30.363	32.0	32.0	32.0	21.0	32.0
3	30.55325	32.0	32.0	32.0	32.0	32.0
4	30.8695	32.0	32.0	32.0	32.0	32.0
5	30.85825	32.0	32.0	32.0	32.0	32.0
6	34.30975	36.0	36.0	36.0	32.0	36.0
7	34.36475	36.0	36.0	36.0	32.0	36.0
8	34.1895	36.0	36.0	36.0	32.0	36.0
9	34.483	36.0	36.0	36.0	32.0	36.0
10	34.162	36.0	36.0	36.0	32.0	36.0
11	34.4885	36.0	36.0	36.0	32.0	36.0
12	34.22075	36.0	36.0	36.0	32.0	36.0
13	34.4235	36.0	36.0	36.0	32.0	36.0
14	34.20475	36.0	36.0	36.0	32.0	36.0
15	34.45075	36.0	36.0	36.0	32.0	36.0
16	34.23575	36.0	36.0	36.0	32.0	36.0
17	34.3605	36.0	36.0	36.0	32.0	36.0
18	34.257	36.0	36.0	36.0	32.0	36.0
19	34.23075	36.0	36.0	36.0	32.0	36.0
20	34.21525	36.0	36.0	36.0	32.0	36.0
21	34.267	36.0	36.0	36.0	32.0	36.0
22	34.0845	36.0	36.0	36.0	32.0	36.0
23	34.06375	36.0	36.0	36.0	32.0	36.0
24	33.95125	36.0	36.0	36.0	32.0	36.0
25	33.9835	36.0	36.0	36.0	32.0	36.0
26	33.829	36.0	36.0	36.0	32.0	36.0
27	33.93975	36.0	36.0	36.0	32.0	36.0
28	33.85975	36.0	36.0	36.0	32.0	36.0
29	33.6405	36.0	36.0	36.0	27.0	36.0
30	33.85975	36.0	36.0	36.0	32.0	36.0
31	33.9425	36.0	36.0	36.0	32.0	36.0
32	33.6505	36.0	36.0	36.0	27.0	36.0
33	33.06675	36.0	36.0	36.0	21.0	36.0
34	31.268	36.0	36.0	36.0	14.0	36.0
35	22.9615	36.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	4.0
24	11.0
25	24.0
26	48.0
27	62.0
28	113.0
29	130.0
30	225.0
31	298.0
32	445.0
33	722.0
34	1030.0
35	886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51112778194549	17.37934483620905	11.77794448612153	26.331582895723933
2	19.825	20.349999999999998	34.0	25.825
3	19.675	24.25	29.825000000000003	26.25
4	13.450000000000001	15.8	38.0	32.75
5	18.075	30.725	31.45	19.75
6	32.22416812609457	36.602451838879155	16.412309231923945	14.761070803102328
7	28.050000000000004	30.25	22.15	19.55
8	26.55	30.825000000000003	19.925	22.7
9	29.5	13.675	19.575	37.25
10	19.8	22.175	27.3	30.725
11	37.95	20.474999999999998	21.425	20.150000000000002
12	27.250000000000004	23.1	25.575	24.075
13	28.199999999999996	18.05	25.35	28.4
14	24.675	21.25	23.25	30.825000000000003
15	27.6	23.45	21.8	27.150000000000002
16	27.725	22.650000000000002	22.825	26.8
17	25.45	24.925	23.674999999999997	25.95
18	27.325	21.45	24.925	26.3
19	27.625	23.125	24.474999999999998	24.775
20	27.3	21.099999999999998	23.474999999999998	28.125
21	30.175	22.575	22.325	24.925
22	27.175	23.625	23.05	26.150000000000002
23	23.525	21.625	25.275	29.575000000000003
24	25.4	24.175	23.7	26.724999999999998
25	24.85	23.45	23.674999999999997	28.025
26	26.85671417854464	24.006001500375092	24.006001500375092	25.131282820705174
27	26.93846923461731	22.611305652826413	22.786393196598297	27.66383191595798
28	24.81240620310155	22.061030515257627	25.962981490745374	27.163581790895446
29	25.962981490745374	21.335667833916958	25.062531265632813	27.63881940970485
30	25.46273136568284	21.260630315157577	27.688844422211105	25.587793896948476
31	28.653653653653656	22.44744744744745	23.1981981981982	25.7007007007007
32	25.256827862691054	22.225006264094212	22.876472062139815	29.64169381107492
33	24.9936175644626	22.823589481746236	25.91268828184835	26.27010467194281
34	26.704545454545453	23.72835497835498	26.650432900432904	22.916666666666664
35	40.9209060527293	31.748978834014114	27.330115113256593	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	3.5
2	2.0
3	2.0
4	4.5
5	7.0
6	7.0
7	8.0
8	9.0
9	9.0
10	6.0
11	3.0
12	3.0
13	2.5
14	2.0
15	2.0
16	6.0
17	10.0
18	10.0
19	12.5
20	15.0
21	23.0
22	31.0
23	31.0
24	31.0
25	31.0
26	31.0
27	40.5
28	50.0
29	50.0
30	77.5
31	105.0
32	105.0
33	119.5
34	134.0
35	134.0
36	164.5
37	195.0
38	195.0
39	233.5
40	272.0
41	263.5
42	255.0
43	255.0
44	317.0
45	379.0
46	379.0
47	352.0
48	325.0
49	325.0
50	318.5
51	312.0
52	312.0
53	322.0
54	332.0
55	332.0
56	325.5
57	319.0
58	319.0
59	304.5
60	290.0
61	275.5
62	261.0
63	261.0
64	229.0
65	197.0
66	197.0
67	179.0
68	161.0
69	161.0
70	118.5
71	76.0
72	76.0
73	62.0
74	48.0
75	48.0
76	45.5
77	43.0
78	43.0
79	37.5
80	32.0
81	29.0
82	26.0
83	26.0
84	26.5
85	27.0
86	27.0
87	25.5
88	24.0
89	24.0
90	16.5
91	9.0
92	9.0
93	11.0
94	13.0
95	13.0
96	6.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.05
28	0.05
29	0.05
30	0.05
31	0.1
32	0.22499999999999998
33	2.075
34	7.6
35	32.675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.10000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3338301043219	54.574999999999996
2	8.79284649776453	11.799999999999999
3	3.8002980625931446	7.6499999999999995
4	1.9374068554396422	5.2
5	0.782414307004471	2.625
6	1.005961251862891	4.05
7	0.856929955290611	4.025
8	0.5961251862891207	3.2
9	0.14903129657228018	0.8999999999999999
>10	0.7451564828614009	5.975
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTT	19	0.475	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG	17	0.42500000000000004	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCN	15	0.375	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG	14	0.35000000000000003	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTN	13	0.325	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	12	0.3	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	12	0.3	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGA	12	0.3	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAG	11	0.27499999999999997	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCN	11	0.27499999999999997	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGG	11	0.27499999999999997	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCG	11	0.27499999999999997	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	11	0.27499999999999997	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG	10	0.25	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATG	10	0.25	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	10	0.25	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCAT	10	0.25	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTA	10	0.25	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	10	0.25	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	9	0.22499999999999998	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA	9	0.22499999999999998	No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCG	9	0.22499999999999998	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	9	0.22499999999999998	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACN	8	0.2	No Hit
GCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCA	8	0.2	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG	8	0.2	No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTN	8	0.2	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGG	8	0.2	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTG	8	0.2	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAA	8	0.2	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATG	8	0.2	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGANN	8	0.2	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	8	0.2	No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA	8	0.2	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTN	8	0.2	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGT	8	0.2	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGG	8	0.2	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGT	8	0.2	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATN	8	0.2	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCNNN	7	0.17500000000000002	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCG	7	0.17500000000000002	No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTNN	7	0.17500000000000002	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTT	7	0.17500000000000002	No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGNN	7	0.17500000000000002	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCN	7	0.17500000000000002	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAA	7	0.17500000000000002	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTG	7	0.17500000000000002	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT	7	0.17500000000000002	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG	7	0.17500000000000002	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAG	7	0.17500000000000002	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA	7	0.17500000000000002	No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCN	7	0.17500000000000002	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGN	7	0.17500000000000002	No Hit
GGTAGGCACGGCGACTACCATCGAAAGTTGATAGG	7	0.17500000000000002	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA	7	0.17500000000000002	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAG	7	0.17500000000000002	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGA	7	0.17500000000000002	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGN	7	0.17500000000000002	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA	7	0.17500000000000002	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAA	7	0.17500000000000002	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTA	7	0.17500000000000002	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTG	7	0.17500000000000002	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG	6	0.15	No Hit
GCGCAATACGAATGCCCCCGGCCGTCCCTCTTAAT	6	0.15	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCG	6	0.15	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACA	6	0.15	No Hit
GATTTAATGAGCCATTCGCAGTTTCACTGTACCGG	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	6	0.15	No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG	6	0.15	No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCN	6	0.15	No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA	6	0.15	No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTAN	6	0.15	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT	6	0.15	No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	6	0.15	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAN	6	0.15	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGN	6	0.15	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCG	6	0.15	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGG	6	0.15	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT	6	0.15	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	6	0.15	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGNNN	6	0.15	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	6	0.15	No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACCCAACACG	6	0.15	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGN	6	0.15	No Hit
GAATTACCGCGGCTGCTGGCACCAGACTTGCCCTN	6	0.15	No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATN	6	0.15	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGA	6	0.15	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG	6	0.15	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCN	5	0.125	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGT	5	0.125	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	5	0.125	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCG	5	0.125	No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGN	5	0.125	No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCN	5	0.125	No Hit
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	5	0.125	No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTAT	5	0.125	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA	5	0.125	No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGN	5	0.125	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGN	5	0.125	No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAA	5	0.125	No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG	5	0.125	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGA	5	0.125	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGT	5	0.125	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT	5	0.125	No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAA	5	0.125	No Hit
CTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCG	5	0.125	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCAT	5	0.125	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTANN	5	0.125	No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.025	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.05	0.0	0.0	0.0	0.0
19	0.05	0.0	0.0	0.0	0.0
20	0.075	0.0	0.0	0.0	0.0
21	0.075	0.0	0.0	0.0	0.0
22	0.075	0.0	0.0	0.0	0.0
23	0.075	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR13210712 read2 length is 35 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210712_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60325	32.0	32.0	32.0	32.0	32.0
2	29.85225	32.0	32.0	32.0	21.0	32.0
3	29.8595	32.0	32.0	32.0	21.0	32.0
4	29.82025	32.0	32.0	32.0	21.0	32.0
5	29.74575	32.0	32.0	32.0	21.0	32.0
6	33.011	36.0	36.0	36.0	21.0	36.0
7	33.0565	36.0	36.0	36.0	21.0	36.0
8	33.0765	36.0	36.0	36.0	21.0	36.0
9	33.466	36.0	36.0	36.0	21.0	36.0
10	32.66625	36.0	32.0	36.0	21.0	36.0
11	33.0685	36.0	36.0	36.0	21.0	36.0
12	33.1345	36.0	36.0	36.0	21.0	36.0
13	33.248	36.0	36.0	36.0	21.0	36.0
14	32.9885	36.0	32.0	36.0	21.0	36.0
15	33.2835	36.0	36.0	36.0	21.0	36.0
16	32.85075	36.0	32.0	36.0	21.0	36.0
17	32.60425	36.0	32.0	36.0	21.0	36.0
18	32.95725	36.0	32.0	36.0	21.0	36.0
19	32.46	36.0	32.0	36.0	21.0	36.0
20	32.5245	36.0	32.0	36.0	21.0	36.0
21	32.3385	36.0	32.0	36.0	14.0	36.0
22	32.178	36.0	32.0	36.0	14.0	36.0
23	31.73675	36.0	32.0	36.0	14.0	36.0
24	31.95675	36.0	32.0	36.0	14.0	36.0
25	32.0105	36.0	32.0	36.0	14.0	36.0
26	32.34375	36.0	32.0	36.0	14.0	36.0
27	32.121	36.0	32.0	36.0	14.0	36.0
28	31.793	36.0	32.0	36.0	14.0	36.0
29	31.9905	36.0	32.0	36.0	14.0	36.0
30	31.773	36.0	32.0	36.0	14.0	36.0
31	32.0605	36.0	32.0	36.0	14.0	36.0
32	31.3405	36.0	32.0	36.0	14.0	36.0
33	30.86375	36.0	32.0	36.0	14.0	36.0
34	28.904	36.0	21.0	36.0	14.0	36.0
35	20.85025	21.0	2.0	36.0	2.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	5.0
17	5.0
18	9.0
19	10.0
20	14.0
21	29.0
22	33.0
23	39.0
24	47.0
25	61.0
26	106.0
27	152.0
28	185.0
29	267.0
30	333.0
31	433.0
32	529.0
33	652.0
34	709.0
35	381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65331664580726	19.198998748435546	12.165206508135169	25.982478097622025
2	17.24224224224224	23.04804804804805	35.96096096096096	23.74874874874875
3	17.788341255941955	26.670002501876404	28.396297222917187	27.145359019264447
4	12.38119059529765	16.18309154577289	36.76838419209605	34.667333666833414
5	14.475	36.625	31.674999999999997	17.224999999999998
6	30.375000000000004	37.025000000000006	18.025	14.575
7	29.15	30.099999999999998	22.225	18.525
8	28.299999999999997	29.2	21.224999999999998	21.275
9	28.95	13.55	20.25	37.25
10	18.45	24.3	26.974999999999998	30.275000000000002
11	38.0	21.675	21.224999999999998	19.1
12	27.55	24.4	26.3	21.75
13	29.975	18.025	24.375	27.625
14	24.025	22.775000000000002	23.5	29.7
15	26.075	26.375	21.525	26.025
16	26.424999999999997	24.675	24.3	24.6
17	24.175	26.575	23.825	25.424999999999997
18	27.075	20.575	26.224999999999998	26.125
19	26.424999999999997	23.875	25.224999999999998	24.474999999999998
20	27.831957989497376	22.005501375343837	24.056014003500874	26.106526631657918
21	29.9074768692173	22.255563890972745	23.305826456614152	24.5311327831958
22	27.406851712928233	22.455613903475868	21.05526381595399	29.08227056764191
23	24.349999999999998	22.875	25.275	27.500000000000004
24	25.074999999999996	26.625	22.3	26.0
25	25.0	22.175	24.55	28.275
26	25.6	24.9	25.25	24.25
27	26.6816704176044	22.980745186296573	22.255563890972745	28.08202050512628
28	24.787393696848426	22.886443221610804	24.96248124062031	27.363681840920464
29	25.581395348837212	21.905476369092273	25.581395348837212	26.93173293323331
30	26.788394197098548	23.06153076538269	24.937468734367183	25.212606303151574
31	28.52852852852853	21.646646646646648	22.822822822822822	27.002002002002
32	25.658389766741912	20.89290193127665	23.97792826686732	29.470780035114117
33	24.04385517593065	23.71239163691994	24.604793472718	27.63895971443141
34	27.285018929150894	23.66143861546782	25.175770686857764	23.877771768523527
35	38.547486033519554	33.29608938547486	28.156424581005584	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	9.5
2	3.0
3	3.0
4	5.5
5	8.0
6	8.0
7	7.5
8	7.0
9	7.0
10	5.5
11	4.0
12	4.0
13	2.5
14	1.0
15	1.0
16	5.0
17	9.0
18	9.0
19	17.5
20	26.0
21	23.0
22	20.0
23	20.0
24	23.5
25	27.0
26	27.0
27	43.5
28	60.0
29	60.0
30	89.5
31	119.0
32	119.0
33	124.0
34	129.0
35	129.0
36	158.0
37	187.0
38	187.0
39	239.0
40	291.0
41	284.0
42	277.0
43	277.0
44	326.0
45	375.0
46	375.0
47	385.0
48	395.0
49	395.0
50	352.5
51	310.0
52	310.0
53	311.5
54	313.0
55	313.0
56	319.0
57	325.0
58	325.0
59	301.5
60	278.0
61	261.0
62	244.0
63	244.0
64	211.5
65	179.0
66	179.0
67	165.5
68	152.0
69	152.0
70	109.5
71	67.0
72	67.0
73	53.0
74	39.0
75	39.0
76	36.5
77	34.0
78	34.0
79	34.0
80	34.0
81	29.0
82	24.0
83	24.0
84	22.5
85	21.0
86	21.0
87	16.0
88	11.0
89	11.0
90	8.0
91	5.0
92	5.0
93	5.5
94	6.0
95	6.0
96	4.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.125
2	0.1
3	0.075
4	0.05
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.025
21	0.025
22	0.025
23	0.0
24	0.0
25	0.0
26	0.0
27	0.025
28	0.05
29	0.025
30	0.05
31	0.1
32	0.325
33	1.95
34	7.55
35	32.875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
35	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19819140919367	53.87499999999999
2	9.080633006782216	12.049999999999999
3	3.541823662396383	7.049999999999999
4	1.9969856819894498	5.3
5	0.9042954031650339	3.0
6	1.092690278824416	4.35
7	0.5275056518462697	2.45
8	0.5275056518462697	2.8000000000000003
9	0.30143180105501133	1.7999999999999998
>10	0.8289374529012812	7.324999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACA	24	0.6	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACN	21	0.525	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG	17	0.42500000000000004	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCG	17	0.42500000000000004	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAA	17	0.42500000000000004	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16	0.4	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	14	0.35000000000000003	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTN	13	0.325	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGG	13	0.325	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATN	13	0.325	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCN	12	0.3	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT	12	0.3	No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCAT	11	0.27499999999999997	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCG	11	0.27499999999999997	No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCN	11	0.27499999999999997	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTT	11	0.27499999999999997	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	10	0.25	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGT	10	0.25	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG	10	0.25	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAG	10	0.25	No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCA	10	0.25	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTN	10	0.25	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCN	9	0.22499999999999998	No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTNNN	9	0.22499999999999998	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATG	9	0.22499999999999998	No Hit
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCN	9	0.22499999999999998	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGT	9	0.22499999999999998	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCG	9	0.22499999999999998	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCAT	9	0.22499999999999998	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCNNN	8	0.2	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA	8	0.2	No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCG	8	0.2	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCG	8	0.2	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTG	8	0.2	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	8	0.2	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT	8	0.2	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGNN	8	0.2	No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGN	8	0.2	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATN	8	0.2	No Hit
ACGCAGAGTACATGGGTACCTGGTTGATCCTGCCA	8	0.2	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGA	8	0.2	No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTT	8	0.2	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATG	8	0.2	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCG	7	0.17500000000000002	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTN	7	0.17500000000000002	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTG	7	0.17500000000000002	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	7	0.17500000000000002	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCG	7	0.17500000000000002	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGANN	7	0.17500000000000002	No Hit
GAATCGAACCCTGATTCCCCGTCACCCGTGGTCAN	7	0.17500000000000002	No Hit
CATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGN	7	0.17500000000000002	No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGN	7	0.17500000000000002	No Hit
GTACATGGGAATAACGCCGCCGCATCGCCGGTCGG	7	0.17500000000000002	No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG	7	0.17500000000000002	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGT	7	0.17500000000000002	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGG	7	0.17500000000000002	No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCG	7	0.17500000000000002	No Hit
GGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGN	6	0.15	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAG	6	0.15	No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCG	6	0.15	No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG	6	0.15	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAA	6	0.15	No Hit
AAAGTGGACTCATTCCAATTACAGGGCCTCGAAAG	6	0.15	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATNN	6	0.15	No Hit
CAATACAGGACTCTTTCGAGGCCCTGTAATTGGAA	6	0.15	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	6	0.15	No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTG	6	0.15	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGN	6	0.15	No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTT	6	0.15	No Hit
CGTTTATGGTCGGAACTACGACGGTATCTGATCGT	6	0.15	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAG	6	0.15	No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCAT	6	0.15	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTA	6	0.15	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	6	0.15	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTT	6	0.15	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCN	6	0.15	No Hit
GTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCN	6	0.15	No Hit
GGATAACTGTGGTAATTCTAGAGCTAATACATGCN	6	0.15	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTA	6	0.15	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGN	6	0.15	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGN	6	0.15	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGT	6	0.15	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCN	6	0.15	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCG	6	0.15	No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGT	6	0.15	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTG	5	0.125	No Hit
TGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTT	5	0.125	No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTT	5	0.125	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAT	5	0.125	No Hit
TATCAACGCAGAGTACATGGGAATAACGCCGCCGN	5	0.125	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG	5	0.125	No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGNN	5	0.125	No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCG	5	0.125	No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCG	5	0.125	No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTAN	5	0.125	No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTG	5	0.125	No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGT	5	0.125	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAA	5	0.125	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAN	5	0.125	No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGN	5	0.125	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG	5	0.125	No Hit
GTGGTAATTCTAGAGCTAATACATGCCGACGGGCG	5	0.125	No Hit
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAA	5	0.125	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	5	0.125	No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGG	5	0.125	No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGG	5	0.125	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG	5	0.125	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAT	5	0.125	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.025	0.0	0.0	0.0	0.0
21	0.025	0.0	0.0	0.0	0.0
22	0.025	0.0	0.0	0.0	0.0
23	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3425 spots for SRR13210712.sra
Written 3425 spots for SRR13210712.sra
Read 3450 spots for SRR13210712.sra
Written 3450 spots for SRR13210712.sra
SRR ids: ['SRR13210712.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_c1c35tdm
SRR13210712.sra spots: 164425
blocks: [[1, 3425], [3426, 6850], [6851, 10275], [10276, 13700], [13701, 17125], [17126, 20550], [20551, 23975], [23976, 27400], [27401, 30825], [30826, 34250], [34251, 37675], [37676, 41100], [41101, 44525], [44526, 47950], [47951, 51375], [51376, 54800], [54801, 58225], [58226, 61650], [61651, 65075], [65076, 68500], [68501, 71925], [71926, 75350], [75351, 78775], [78776, 82200], [82201, 85625], [85626, 89050], [89051, 92475], [92476, 95900], [95901, 99325], [99326, 102750], [102751, 106175], [106176, 109600], [109601, 113025], [113026, 116450], [116451, 119875], [119876, 123300], [123301, 126725], [126726, 130150], [130151, 133575], [133576, 137000], [137001, 140425], [140426, 143850], [143851, 147275], [147276, 150700], [150701, 154125], [154126, 157550], [157551, 160975], [160976, 164425]]
SRR13210712 file size 1
SRR13210712 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13210712 SRR13210712_1.fastq SRR13210712_2.fastq
Input file:	SRR13210712_1.fastq
Paired file:	SRR13210712_2.fastq
trimmed:	SRR13210712-trimmed-pair1.fastq, SRR13210712-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Fri May  6 12:12:54 2022 >> started

Fri May  6 12:12:55 2022 >> done (0.245s)
164425 read pairs processed; of these:
     0 ( 0.00%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
164425 (100.00%) read pairs available; of these:
 90560 (55.08%) trimmed read pairs available after processing
 73865 (44.92%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 21	     1	  0.00%
 22	     0	  0.00%
 23	     0	  0.00%
 24	     1	  0.00%
 25	     0	  0.00%
 26	     1	  0.00%
 27	     0	  0.00%
 28	     3	  0.00%
 29	    15	  0.01%
 30	   107	  0.07%
 31	    62	  0.04%
 32	   744	  0.45%
 33	 11036	  6.71%
 34	 78590	 47.80%
 35	 73865	 44.92%
164425 reads passed initial QC


criterion=sequence-density
sequence-density=1.02
sequence-density-rank=1
fanout-score=3.13
fanout-score-rank=19
prefix-density=3.19
prefix-fanout=1.0
sequence=GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=31
fanout-score=30.99
fanout-score-rank=1
prefix-density=3.19
prefix-fanout=1.0
sequence=GTACATGGGGTG


criterion=sequence-density
sequence-density=1.25
sequence-density-rank=1
fanout-score=2.68
fanout-score-rank=20
prefix-density=3.36
prefix-fanout=1.0
sequence=GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=32
fanout-score=32.47
fanout-score-rank=1
prefix-density=3.36
prefix-fanout=1.0
sequence=GTACATGGGGGC
SRR13210712 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 06 12:13:48
                             Started mapping on |	May 06 12:13:49
                                    Finished on |	May 06 12:14:02
       Mapping speed, Million of reads per hour |	45.53

                          Number of input reads |	164425
                      Average input read length |	45
                                    UNIQUE READS:
                   Uniquely mapped reads number |	14134
                        Uniquely mapped reads % |	8.60%
                          Average mapped length |	44.54
                       Number of splices: Total |	476
            Number of splices: Annotated (sjdb) |	462
                       Number of splices: GT/AG |	468
                       Number of splices: GC/AG |	2
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	6
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	93192
             % of reads mapped to multiple loci |	56.68%
        Number of reads mapped to too many loci |	2391
             % of reads mapped to too many loci |	1.45%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	32.55%
                     % of reads unmapped: other |	0.72%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	57099	57099	57099
N_multimapping	93192	93192	93192
N_noFeature	5077	9591	9421
N_ambiguous	433	119	124
UnstrandedReadsAssigned:8624 PositiveStrandReadsAssigned:4424 NegativeStrandReadsAssigned:4589
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13210712 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 180,869
[index] number of k-mers: 102,188,183
[index] number of equivalence classes: 1,184,234
[quant] running in paired-end mode
[quant] will process pair 1: SRR13210712-trimmed-pair1.fastq
                             SRR13210712-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 164,425 reads, 45,313 reads pseudoaligned
[quant] estimated average fragment length: 103.728
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 586 rounds

 180870 SRR13210712.ke.tsv
  58302 SRR13210712.se.tsv
 239172 total
==> SRR13210712.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	10	0	0
ENST00000448914.1	13	14	0	0
ENST00000415118.1	8	9	0	0
ENST00000631435.1	12	13	0	0
ENST00000632684.1	12	13	0	0
ENST00000454908.1	17	1	0	0
ENST00000390567.1	20	2.33333	0	0
ENST00000603326.1	19	2.66667	0	0
ENST00000414852.1	16	17	0	0

==> SRR13210712.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210712 completed mapping pipeline successfully
