Starting /dee2/code/volunteer_pipeline.sh SRR13210229
    current disk space = 2143527356
    free memory = 122217108 
SRR13210229 SRAfilesize
a3cad70b5ac4822bc6684ae68db8912d  SRR13210229.sra
SRR13210229.sra file validated
SRR13210229 is single end
SRR13210229 is conventional basespace
SRR13210229 read1 length is 41-71 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13210229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179
Sequences flagged as poor quality	0
Sequence length	41-71
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.910614525139664	32.0	32.0	32.0	32.0	32.0
2	30.910614525139664	32.0	32.0	32.0	32.0	32.0
3	31.11173184357542	32.0	32.0	32.0	32.0	32.0
4	31.050279329608937	32.0	32.0	32.0	32.0	32.0
5	30.787709497206706	32.0	32.0	32.0	32.0	32.0
6	34.324022346368714	36.0	36.0	36.0	32.0	36.0
7	34.14525139664804	36.0	36.0	36.0	32.0	36.0
8	34.18994413407821	36.0	36.0	36.0	32.0	36.0
9	34.79329608938548	36.0	36.0	36.0	32.0	36.0
10	33.770949720670394	36.0	36.0	36.0	21.0	36.0
11	34.08379888268156	36.0	36.0	36.0	32.0	36.0
12	34.134078212290504	36.0	36.0	36.0	32.0	36.0
13	34.79329608938548	36.0	36.0	36.0	32.0	36.0
14	33.787709497206706	36.0	36.0	36.0	21.0	36.0
15	34.212290502793294	36.0	36.0	36.0	32.0	36.0
16	33.16201117318436	36.0	36.0	36.0	14.0	36.0
17	33.63687150837989	36.0	36.0	36.0	21.0	36.0
18	33.8268156424581	36.0	36.0	36.0	32.0	36.0
19	34.16201117318436	36.0	36.0	36.0	32.0	36.0
20	34.737430167597765	36.0	36.0	36.0	32.0	36.0
21	34.11173184357542	36.0	36.0	36.0	32.0	36.0
22	34.245810055865924	36.0	36.0	36.0	32.0	36.0
23	33.977653631284916	36.0	36.0	36.0	32.0	36.0
24	34.184357541899445	36.0	36.0	36.0	32.0	36.0
25	33.29050279329609	36.0	36.0	36.0	21.0	36.0
26	33.687150837988824	36.0	36.0	36.0	21.0	36.0
27	33.039106145251395	36.0	36.0	36.0	14.0	36.0
28	34.0	36.0	36.0	36.0	32.0	36.0
29	32.927374301675975	36.0	36.0	36.0	14.0	36.0
30	32.98882681564246	36.0	36.0	36.0	14.0	36.0
31	33.26815642458101	36.0	36.0	36.0	21.0	36.0
32	33.12849162011173	36.0	36.0	36.0	14.0	36.0
33	33.29608938547486	36.0	36.0	36.0	14.0	36.0
34	32.977653631284916	36.0	32.0	36.0	21.0	36.0
35	34.30167597765363	36.0	36.0	36.0	32.0	36.0
36	33.642458100558656	36.0	32.0	36.0	32.0	36.0
37	33.94972067039106	36.0	36.0	36.0	32.0	36.0
38	32.770949720670394	36.0	32.0	36.0	14.0	36.0
39	33.312849162011176	36.0	36.0	36.0	21.0	36.0
40	32.960893854748605	36.0	36.0	36.0	21.0	36.0
41	33.05027932960894	36.0	36.0	36.0	21.0	36.0
42	33.90449438202247	36.0	36.0	36.0	32.0	36.0
43	33.42696629213483	36.0	36.0	36.0	21.0	36.0
44	32.78531073446328	36.0	32.0	36.0	14.0	36.0
45	33.63068181818182	36.0	36.0	36.0	21.0	36.0
46	33.04545454545455	36.0	36.0	36.0	14.0	36.0
47	32.97142857142857	36.0	32.0	36.0	21.0	36.0
48	33.537142857142854	36.0	36.0	36.0	21.0	36.0
49	33.08571428571429	36.0	36.0	36.0	14.0	36.0
50	32.66285714285714	36.0	32.0	36.0	14.0	36.0
51	33.589595375722546	36.0	36.0	36.0	21.0	36.0
52	32.86705202312139	36.0	32.0	36.0	14.0	36.0
53	32.445086705202314	36.0	32.0	36.0	14.0	36.0
54	32.6242774566474	36.0	32.0	36.0	14.0	36.0
55	32.824561403508774	36.0	36.0	36.0	14.0	36.0
56	32.421052631578945	36.0	32.0	36.0	14.0	36.0
57	33.2046783625731	36.0	36.0	36.0	21.0	36.0
58	32.60233918128655	36.0	32.0	36.0	14.0	36.0
59	32.280701754385966	36.0	32.0	36.0	14.0	36.0
60	32.15204678362573	36.0	32.0	36.0	14.0	36.0
61	33.228070175438596	36.0	36.0	36.0	21.0	36.0
62	32.78947368421053	36.0	36.0	36.0	14.0	36.0
63	31.555555555555557	36.0	32.0	36.0	14.0	36.0
64	33.25730994152047	36.0	36.0	36.0	21.0	36.0
65	32.666666666666664	36.0	32.0	36.0	14.0	36.0
66	33.31578947368421	36.0	36.0	36.0	21.0	36.0
67	32.707602339181285	36.0	36.0	36.0	14.0	36.0
68	33.26470588235294	36.0	36.0	36.0	21.0	36.0
69	32.981818181818184	36.0	36.0	36.0	14.0	36.0
70	30.65771812080537	36.0	32.0	36.0	14.0	36.0
71	29.865384615384617	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	0.0
26	2.0
27	5.0
28	5.0
29	7.0
30	8.0
31	16.0
32	14.0
33	32.0
34	51.0
35	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.87150837988827	19.553072625698324	11.731843575418994	31.843575418994412
2	14.52513966480447	20.670391061452513	37.988826815642454	26.81564245810056
3	17.318435754189945	24.581005586592177	25.139664804469277	32.960893854748605
4	12.849162011173185	15.083798882681565	40.78212290502793	31.28491620111732
5	18.994413407821227	32.960893854748605	29.608938547486037	18.435754189944134
6	31.28491620111732	37.988826815642454	17.877094972067038	12.849162011173185
7	27.37430167597765	32.402234636871505	19.553072625698324	20.670391061452513
8	21.787709497206702	32.402234636871505	21.22905027932961	24.581005586592177
9	22.905027932960895	15.083798882681565	18.994413407821227	43.01675977653631
10	16.75977653631285	24.581005586592177	24.022346368715084	34.63687150837989
11	35.19553072625698	19.553072625698324	27.37430167597765	17.877094972067038
12	21.22905027932961	18.994413407821227	25.69832402234637	34.07821229050279
13	25.69832402234637	18.994413407821227	29.608938547486037	25.69832402234637
14	22.905027932960895	20.670391061452513	20.11173184357542	36.312849162011176
15	27.37430167597765	27.37430167597765	18.994413407821227	26.256983240223462
16	31.843575418994412	31.28491620111732	17.877094972067038	18.994413407821227
17	20.11173184357542	24.581005586592177	29.05027932960894	26.256983240223462
18	26.256983240223462	27.37430167597765	20.670391061452513	25.69832402234637
19	26.256983240223462	24.581005586592177	22.905027932960895	26.256983240223462
20	22.3463687150838	26.256983240223462	26.256983240223462	25.139664804469277
21	31.28491620111732	18.994413407821227	20.670391061452513	29.05027932960894
22	29.05027932960894	25.139664804469277	23.463687150837988	22.3463687150838
23	23.463687150837988	24.022346368715084	20.670391061452513	31.843575418994412
24	29.608938547486037	26.81564245810056	21.787709497206702	21.787709497206702
25	29.05027932960894	26.256983240223462	24.581005586592177	20.11173184357542
26	27.932960893854748	23.463687150837988	24.022346368715084	24.581005586592177
27	24.581005586592177	25.139664804469277	20.11173184357542	30.16759776536313
28	24.581005586592177	22.3463687150838	26.81564245810056	26.256983240223462
29	21.22905027932961	23.463687150837988	30.16759776536313	25.139664804469277
30	25.139664804469277	24.022346368715084	24.022346368715084	26.81564245810056
31	27.932960893854748	21.787709497206702	24.581005586592177	25.69832402234637
32	22.3463687150838	22.905027932960895	20.11173184357542	34.63687150837989
33	22.905027932960895	17.318435754189945	26.81564245810056	32.960893854748605
34	27.37430167597765	26.256983240223462	25.139664804469277	21.22905027932961
35	27.932960893854748	18.435754189944134	26.256983240223462	27.37430167597765
36	23.463687150837988	30.726256983240223	17.877094972067038	27.932960893854748
37	22.3463687150838	24.581005586592177	24.022346368715084	29.05027932960894
38	25.69832402234637	27.932960893854748	27.932960893854748	18.435754189944134
39	31.28491620111732	24.022346368715084	18.435754189944134	26.256983240223462
40	22.3463687150838	29.05027932960894	22.905027932960895	25.69832402234637
41	20.670391061452513	22.905027932960895	25.69832402234637	30.726256983240223
42	23.595505617977526	24.719101123595504	20.786516853932586	30.89887640449438
43	29.775280898876407	20.786516853932586	23.03370786516854	26.40449438202247
44	27.11864406779661	24.858757062146893	18.64406779661017	29.37853107344633
45	19.318181818181817	20.454545454545457	30.681818181818183	29.545454545454547
46	26.136363636363637	23.295454545454543	21.59090909090909	28.97727272727273
47	30.857142857142854	20.57142857142857	23.42857142857143	25.142857142857146
48	30.857142857142854	25.142857142857146	20.57142857142857	23.42857142857143
49	24.0	30.28571428571429	21.714285714285715	24.0
50	30.28571428571429	25.142857142857146	22.857142857142858	21.714285714285715
51	25.43352601156069	25.43352601156069	19.07514450867052	30.057803468208093
52	22.54335260115607	21.38728323699422	29.47976878612717	26.589595375722542
53	27.74566473988439	21.965317919075144	24.85549132947977	25.43352601156069
54	24.277456647398843	19.653179190751445	23.121387283236995	32.947976878612714
55	27.485380116959064	24.561403508771928	28.07017543859649	19.883040935672515
56	28.07017543859649	27.485380116959064	23.391812865497073	21.052631578947366
57	26.900584795321635	19.883040935672515	22.807017543859647	30.409356725146196
58	29.239766081871345	25.146198830409354	20.46783625730994	25.146198830409354
59	29.82456140350877	16.95906432748538	26.900584795321635	26.31578947368421
60	23.391812865497073	23.391812865497073	24.561403508771928	28.654970760233915
61	25.730994152046783	22.807017543859647	16.95906432748538	34.50292397660819
62	22.807017543859647	26.900584795321635	23.976608187134502	26.31578947368421
63	25.146198830409354	17.543859649122805	28.654970760233915	28.654970760233915
64	26.31578947368421	23.391812865497073	24.561403508771928	25.730994152046783
65	25.146198830409354	23.976608187134502	29.239766081871345	21.637426900584796
66	27.485380116959064	32.748538011695906	18.128654970760234	21.637426900584796
67	22.807017543859647	28.654970760233915	20.46783625730994	28.07017543859649
68	22.941176470588236	30.0	22.941176470588236	24.11764705882353
69	31.515151515151512	18.181818181818183	32.121212121212125	18.181818181818183
70	28.859060402684566	22.14765100671141	27.516778523489933	21.476510067114095
71	39.42307692307692	35.57692307692308	25.0	0.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	0.5
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.5
29	1.5
30	2.0
31	4.0
32	3.0
33	1.5
34	1.0
35	4.5
36	5.0
37	3.0
38	3.5
39	4.5
40	8.5
41	10.0
42	9.5
43	8.0
44	6.5
45	6.5
46	8.0
47	9.0
48	6.5
49	6.0
50	4.5
51	4.5
52	7.0
53	7.0
54	4.0
55	2.0
56	5.5
57	8.5
58	11.0
59	12.0
60	10.0
61	7.0
62	5.0
63	6.5
64	8.0
65	6.0
66	5.5
67	7.0
68	6.0
69	5.0
70	4.0
71	2.5
72	2.0
73	2.0
74	1.5
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
41	1.0
42	0.0
43	1.0
44	1.0
45	0.0
46	1.0
47	0.0
48	0.0
49	0.0
50	2.0
51	0.0
52	0.0
53	0.0
54	2.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	1.0
68	5.0
69	16.0
70	45.0
71	104.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.88268156424581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.87005649717514	97.76536312849163
2	1.1299435028248588	2.2346368715083798
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	2	1.1173184357541899	RNA PCR Primer, Index 46 (96% over 30bp)
CCCAAAGAGCTTGGATTTCAGGCATGAGCTACCACACCTGGCCTAATATGTGTCTACTGAAGTGGAATTAT	2	1.1173184357541899	No Hit
CTTCCATCTCCTCAACGAGGACCGTCGGTACGTGTTCGTGGTCGAAGGTGACGACATCGCACCCGGCGGCG	1	0.5586592178770949	No Hit
CCTTCCATCCTGGGCAAAACTGAAAGGGACCCGGAGCTGGGAAACCCACGGTGGTCTCCAGGCCCTGG	1	0.5586592178770949	No Hit
CGCTAGCAAATCGAACGTAGGCGACATCATCAATTTGGCTCAACTCTTTCATGACGATTTCACCTAAAATT	1	0.5586592178770949	No Hit
GTTTAACAATGTCGTGGCGAGCCGTCAGTCCGCGTGCGAAACGAATCGCTGTCGGGTCCCCCTCGGACTTT	1	0.5586592178770949	No Hit
ACCTCGCATGACGTCAAGCTCGGCGTGCGCTGGAATCTCGAAGCGCCGCCGGCCTATGCACCGCCGCCGC	1	0.5586592178770949	No Hit
TTCAAGACCCTGTCAGGCTGGAGAGAACTTGCTTTCTTATTCTCTCTCCCCTCC	1	0.5586592178770949	No Hit
GCTCATCTCGATGCCTGCGATGTGTCTGATTATGATCGCCTCGCGGGCCTTGTGGCTTCGCGGTGAGTCGG	1	0.5586592178770949	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCAGTA	1	0.5586592178770949	No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGG	1	0.5586592178770949	No Hit
AGCATGGTAAGCTCTAATGAGAAATATATCTAGATAACATTTTCTTAAAAATTGTAGCCAAATTATGG	1	0.5586592178770949	No Hit
GTGTCACCCCGATCGCCTCCCCCCGGTTCTCGAAGGGAAAGACCTCCGCCCCTCCGGGCAGTGCTGACAC	1	0.5586592178770949	No Hit
TGGTTGTAGATATGTGACATTATTTCTGAGGGCTCAGTTCTGTTCCACTGGGCTATATCTCTGTTTTGGTA	1	0.5586592178770949	No Hit
GTGTAATGCCTCCCAGGACTTCCCCGTTCAACTCTCATGACTCTCAACAATAGCCTGAGATCATAAAGTGG	1	0.5586592178770949	No Hit
TCCACGACGTGACGACCGGTGACGACGGGCGGCGGCGATATCAGTCGCTGCCCGAGCGTTACTGCGCGAA	1	0.5586592178770949	No Hit
GCACAACTCGTATCAGTCATTCTTATCGTTGTTAGCATTATTCTTATTGTATATAGAAGAGTTAAGTATCA	1	0.5586592178770949	No Hit
GATCGTGAGATTGGCCGAACTCCAGCGTGGCGCCTTTGATCACCCGATAGTCGGCAAATCGGACCTTTGG	1	0.5586592178770949	No Hit
CCCATATGGCATCAAGTGCGGTCAGGTCGGTCGGATCATCGTCGGGCTGTGCCAACATTCCGGCGACTGCG	1	0.5586592178770949	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	1	0.5586592178770949	No Hit
ACCTATATTTCTTTTTTTTTTTGTGGGGGCACTGGGGGGACATGGGACCACTCCATCTGTCCCCCAGG	1	0.5586592178770949	No Hit
GATCCGGTCGCCGTGCCCGAAAGCGCCGAGGAAATCGAGAAGAAGCGCGAGCGCGTCGACGCCATCCTGCG	1	0.5586592178770949	No Hit
CTGCTGCTTTGCGAAGAAGGTGCTTGCTTCTGCTTTGCCTTCTGCCATGATTGTAAGTTTCCTGAGGCCT	1	0.5586592178770949	No Hit
CTGCAATGCAACGGGACGATCGGCTCCCTATACCAGTTTCGTGGTGGCGCTCGGTATGCCTACGGATTAGG	1	0.5586592178770949	No Hit
TTGCGGGTCCGCTTGCGCTGGCCCAGGCCTTCGGCATCACCGAACGTCAGTTGCATCGATCGTCATCCAAT	1	0.5586592178770949	No Hit
GAATTGCCATTGCAGTACATGAGTCCGCAAACGCGGCTCCTGGGGCAGGAATGTGCTGCCTTCTGCCAGGT	1	0.5586592178770949	No Hit
ATCCAAGATGATCTGCTGCGAGGTGGGATCGTCCGCCAGATCCCACAGCGGACCGTGGCCACCCGGGT	1	0.5586592178770949	No Hit
TATCCAAACTGAGAGGCAAGGAAGCTGAGGCACTTATCCAACCACTCTCCATCTGTTGTTGGTTAAAAGT	1	0.5586592178770949	No Hit
GGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTC	1	0.5586592178770949	No Hit
TTGCCAAGCCTGCTTTCGCAGCACCGAGCACGAGTGGCACCTCGTCCGCGCCGGTCGCGTCGTGCAATGG	1	0.5586592178770949	No Hit
ATAGAAGCCGCCATGCTTCATTGCGGTCTCATGGCGCAGCAGCCGATAGGTGCCGACCACCGGCTGCCGG	1	0.5586592178770949	No Hit
ATCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCCCCTCTGACTTAAAAACCCGCCTACGCACCCTTTA	1	0.5586592178770949	No Hit
GTAGATAAGCTGCAAATTTTAAAAGTATTTAGTTCTCTTTACCTTTTAATTAGTAATTCCGGCTTTCAGT	1	0.5586592178770949	No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTTCAGCTTTGCAACCATACTCCCCCCGGAAC	1	0.5586592178770949	No Hit
CCTGGGTCCAAGTGAGTCTCCTGCCTCAGCCTCTCAAGTAGCTG	1	0.5586592178770949	No Hit
CGGTATCTGATCGTCTTCGAACCTCCGACTTTCGATCTTGATTAATGAAAACATTCTTGGCAAATGCTTT	1	0.5586592178770949	No Hit
CTTCTGTTGTCGGTTTTGATATGGAATTTCCCCTTTGAGAGCTTCATGTGCCCTAGTGCTGGCACTAGGT	1	0.5586592178770949	No Hit
CCGCGGGCACACGACTCCCAGCGCTTGGAGCCTCTGCGGTGTTAGGATGACAGGACTGTCGCCTGGCCGGG	1	0.5586592178770949	No Hit
CTCCAAGGAGGTCCGTGTGAGAGAGTTGTGATCGATTGAGTAAGCAGGGGGTATGTGACTGTGGGCTGCAT	1	0.5586592178770949	No Hit
GTCATCGTGAGCTAAGATGACGATGCCCTCTCATATGAGCTCATCACTTCGGACGGTGGTGCGTTCGATGT	1	0.5586592178770949	No Hit
TGACAATTCTTCACAGGAGTCGGCATGTCCTCATCCACGATGGCTAGTCGCAAGGCCAGACACGAGGCG	1	0.5586592178770949	No Hit
CCCGGATCTCCTTGATGCCCTGGATATTCTCCCACGCGGTGAACGACATCTCGAGCGAGGCCTCCTGGAAG	1	0.5586592178770949	No Hit
GGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTA	1	0.5586592178770949	No Hit
ATCTTCAAGGGCGACCGCCCGCAGGACCAGTTTATAGCCGCCGCCGGCCTGCGCCATGGCCTGGCGTCGT	1	0.5586592178770949	No Hit
ACATAAAATAACAAAGTACACAAACTATAAATTATATACTGTACATAAAATATGAAATTACATCAAATATA	1	0.5586592178770949	No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC	1	0.5586592178770949	No Hit
GCTCTTCCTCCAAGTCAGCTAAGGTGGATACATAAGAGAACCTCAATAAACATTTGCCACAAAGAGGAAAG	1	0.5586592178770949	No Hit
TTTTGAGGGTACTTCAGGAAAAGGAATTCGAGCCGCTGGGGTCCAACGAGGTGCTGCGCTGCGACGTTCGG	1	0.5586592178770949	No Hit
GCCCACACTGGCCACAACCAGCATGCGCTTTCGCCCTACATGATCAGAGAGCCAACCCATCACGAAGATTG	1	0.5586592178770949	No Hit
TAGTTTATTTTAATGGGGAAATAATTCATTGATTACTTGTCTGTTTTTCACTGAGGCAGGTGGAGTGGTGT	1	0.5586592178770949	No Hit
ACCCCAGAGAGACCGACGAAACTGCGCTTGTCGGGCGTCATGGCAAGGTTCTCAGTGCCGTACAGAGTGCA	1	0.5586592178770949	No Hit
CTCAGAAACTTCTCTGTGATGTTTGCATTAAACTCATAGAGTTGAACGCTTCCCTTCATACAGCAGGTTTG	1	0.5586592178770949	No Hit
CTTGAATGTAGAAAGAATAATTTCACATACAGAAAGGAACAATACCTAGAACAGAATAGGGTGTAGAGTT	1	0.5586592178770949	No Hit
CTGCCGAGCAGGTGATGCGAGCCACCCGCGGTGCTGGACTTGGGGCCGAGGCCAAGCGTCGCATCATCTTG	1	0.5586592178770949	No Hit
CTCCGGGCCGACGACGACGAGGTCAGCTTCCAGGCCACGCGCCAAGGAGACAACGGCATCACAATCGCACG	1	0.5586592178770949	No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCATTTAACGAGGATCCATTGGAGGGCAAGT	1	0.5586592178770949	No Hit
TTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTGGCTCGTA	1	0.5586592178770949	No Hit
CTCACGGACGATCTCCGGTTGACGAGTCCTTACGGCACCGGAACCGAGACGCTTGCGCCGTTCGGAATTGG	1	0.5586592178770949	No Hit
TGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCCGGTCCGTCTTGCGCCGGTCCAAGAATTTCAC	1	0.5586592178770949	No Hit
CCTTTGAGAAGGATCACTTTCCAGGCATCGCCGCCAGGGAAGAGCTGGCCAGAGAGACGGGCATCCCAGAG	1	0.5586592178770949	No Hit
GTGAAGAAGTCCGGCGCAAAGCCGAGCTTGAGCGCGAAGCCCGCCAGCACCTTCATGCCGACCGCCCAGAT	1	0.5586592178770949	No Hit
CTGTTCCTCACCGGTTTGGGCTCGGCTCTTGCCAGTGGCAGCGGAAAGACCCGTCCAGTCGCCCGCAATAT	1	0.5586592178770949	No Hit
CATATTACTTCATCAACCAAGTACTCAGCACAGTACCTGAGAGGTATTTTTTTCTGATCCTCTCCCACAT	1	0.5586592178770949	No Hit
CATATATACCATGGAATATTATGCAGCCTTAAAAAAGAACAAAATCATGTCCTTTGCTGCAACATGGATGG	1	0.5586592178770949	No Hit
TCCTTGAGCAGTTCGAAGCCCGGGATCATGCCCTTGAGCGCATTCATGATCTGCTCGCCGCGCGCGGCGGA	1	0.5586592178770949	No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA	1	0.5586592178770949	No Hit
ATCTTTTCCCCACCTTTCCCCGCTTTCTATTCCACAAACCCACCATTGTCATCATGGCCCGTTCTCAATGA	1	0.5586592178770949	No Hit
ATTATTATGCTGACAAAAAGGCATAGATCACCAGGGTCATCTTTTCCGTTTGCTTTCACAAACATTTTGCA	1	0.5586592178770949	No Hit
CTTCAGAAATGTGGGGAAAAATTTGAGAGACATAGAGAATAGT	1	0.5586592178770949	No Hit
CCTGGGTCACGACGTGATCGAAAAGCCGCCCTTTTTCATGAAGCAGGGCGGCCCGACGGTGATCCATGTGT	1	0.5586592178770949	No Hit
GCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCG	1	0.5586592178770949	No Hit
ACCTCGCGCTGGTTCACCAGCGCGAGCTTGCTCTGGTGCAC	1	0.5586592178770949	No Hit
GGATCCGGCCACCCGTCCAAGTGCCAGCCAGCTTGATCGGCTAGGCATCGTCAAGGAGCGACGATGAGGTG	1	0.5586592178770949	No Hit
GAGTAAACCATTTCTTTACTCACTTTCAAGGAAAGCCTGTTCAATTCTTTTCGTTTTTCTTTCAGGGATCA	1	0.5586592178770949	No Hit
TCCAGGTACTGAGGCAGTCTCGTAAGCCCGTCGTGGTAGCTGCCAACAAGGTCGACAATGCACGCGGCGAG	1	0.5586592178770949	No Hit
ATATATGACTTCAATTGTGTTGTCTGAGCAATCTCGTTTCTCGCAAATACATTCTGCGGACTATTGTATT	1	0.5586592178770949	No Hit
CGGCGGCACCGGCGGTGATCTCGAAGGGGTGCTGCCCGGATCGCGGGCTCATGGGCGCGACAGTAGGCC	1	0.5586592178770949	No Hit
ATCGCGACGCCATCAACGAAGTCGCCGGCGCCGGCATCTTCGCGCCGGCCCAACAGCCGTTCACGCCGGC	1	0.5586592178770949	No Hit
GTCCAGGAGCTGCCCGCCGTCGAGGGCCTGGCCCGCGTCGATGTCGTATGCCGCGACAAGACTGGCACT	1	0.5586592178770949	No Hit
GATGTTCAGATGTGATTGGTTAAGTCCATTGACCTGGCTTTTGACATACACGAATCCCCAGAGGGAATTTT	1	0.5586592178770949	No Hit
CCACCTTCCAGGTTCAAGAGATTCTCTTGCCTCAGCCTCCCAAGTAGCTGAGATTACAGGCATGCACCAC	1	0.5586592178770949	No Hit
AGCTGAGTCATTCTTATCCACCCAACCAAAATAGAGTGGAGGGCTGGGGGGAAAGAGCACTCAGGACATG	1	0.5586592178770949	No Hit
CTCTGCGGCAGGGCGTACCAACAGCCCGGGGCCAGACGAGCCGGAACCAGAAAGTCGCGAGCGCCCTCAGG	1	0.5586592178770949	No Hit
CACTCATCCCGATGACCTCGGGAAGCTCGCTAGAAACCATAAGGACGGCGCCCCCGGCGTCGGTAACAGCA	1	0.5586592178770949	No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1	0.5586592178770949	No Hit
GAGCAAATGCCCTGCCTTTCTCAATCAAGTTTATCATCTCACAAAGGAAAACTCCAAAGCCAAAACAGG	1	0.5586592178770949	No Hit
CTCTTACCTCTATATTTCTTTTCCTTACTGTCTTTGAAGATGTGTCCTATTTTCAAGTCAGGGTTCAGAAT	1	0.5586592178770949	No Hit
GAATCCCCGCATGCCGGAGCCGTCCGGGAATGCCGGGAGGAGTTAGGAACCCCCCTTGACATTCCGGGCA	1	0.5586592178770949	No Hit
CTGCCAGGTTATACATTAGTTGCAGTCAATCTTGTAGTCTGTTGCGGGAACTAAGGGACCCCGAATGGAGG	1	0.5586592178770949	No Hit
GAATTGACGCGGCTGAATTCCGAACTGGCGCTGGCCAAGAGCACGGCCGAGGACGCCAACATCTCGAAGA	1	0.5586592178770949	No Hit
GGGCTCGCCGCCTCGGTGGTGACGGCAACGGCGGTGCTGCTGACGTAGTTGATTGCTGGAAATGTGTTTTT	1	0.5586592178770949	No Hit
GCATATGGATTTCCGTTGGCAGTGGGAATGGACATATCAACGATGCAGCGATCCAGAGAGGTAATAGACA	1	0.5586592178770949	No Hit
TAGAACGACGGCTCCGCCGGCGGATAGATCTCCGGGCACACATTGCTGCGCGCCACCGGATTGTTGTCG	1	0.5586592178770949	No Hit
ACCCGACCCCACAGGAAATGCTTTTCAGAAGAGAAAAACACAAAGTGAGAGTTGCATCTCTCAGCTCCCAT	1	0.5586592178770949	No Hit
GAAATCCCACTTTAAACATTAGTAAAGTTAAGTTTATTGCCCCTTCATAGGATATCAAGCCACATATATGT	1	0.5586592178770949	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCT	1	0.5586592178770949	No Hit
GCATAAAACCGTCGTATCCGCTTGAACGGAATTTTGAGGATGCAAGGTAGTTTTCGGAAACATATTCGTC	1	0.5586592178770949	No Hit
TTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTT	1	0.5586592178770949	No Hit
GTATGCCGCAATAGTGCATCGACAACCTTCCACGCCATGGACGATCCCGAGCGCAACCTCGACGTCCAG	1	0.5586592178770949	No Hit
GTGTGTATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTTCGGTTGGCTCCAAGTCTTTG	1	0.5586592178770949	No Hit
AACTAGGCCTATTTTGCTAATGTCAGCAAAGAATGCTGACATTTAGCACAAGCATTTATAGCATCCACTGA	1	0.5586592178770949	No Hit
CGACTGGATCGCCGGCGTCGCGCAGATCGACGAAGTTGATGCCTTTGACGACGCGGCCGTCCTTGACGTCG	1	0.5586592178770949	No Hit
AACGTACTTTGTAACACCACACCTCCGTTAAGGTCACTCACAAAGGGAATGGCTTAACTTCAGTGGAGCGT	1	0.5586592178770949	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAA	1	0.5586592178770949	No Hit
ACAAGAAGAAATTAAACGGTTAGAAACATTTGTAGAAAAAAATATCACAAGAGCTTCTACAAGTGGAATGG	1	0.5586592178770949	No Hit
GCATGCTGGCCATGGGTGCGATGTTCGGTGTGCTTGCTTACCTGCCGACCTATATGCAGATGACCTATGG	1	0.5586592178770949	No Hit
ATATAATATGTAGAAAAATGTCAGTAGTAAGAACACTGAATGTCAAAATTTATGAGACTTCCAAAGTTGGA	1	0.5586592178770949	No Hit
CCGTCATCTCTTTTAGGAATACCGTATACCAATGGTGGCTCCCTGATCAAAAAAGTGATTTTTTAAAATTT	1	0.5586592178770949	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGA	1	0.5586592178770949	No Hit
ACATAGCCGCCGACCCCAAACACCAGCTCCGGCCGGAAACGCCGCAGCATCCCCACGGCCTGCCAAACAG	1	0.5586592178770949	No Hit
GCTCTGGGTTAGTCGGGTCCTAAGCTGCGGCCGACAGGCGTAGGCGATGGATAACAGGTTGATATTC	1	0.5586592178770949	No Hit
AGATGGGAAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACATGCTGACTGTCCT	1	0.5586592178770949	No Hit
ATGGCGTCCTCATTGCCGTATGTGCTGCGCAGCTCATCGATAGGTCCCCAATCCAGAGCTCGCGACGGGCA	1	0.5586592178770949	No Hit
GCCTTAGCTCGCATTCAAGGCGTTAATCCGGCTCGCCAGCGCTTGTTTTTTATGGGAATACTGGCAGGGTT	1	0.5586592178770949	No Hit
CTATAGCTCACTTAGATGGCTTGGGTAAAAGTGCCTCTTAATTGTTGAATTCACTGCTGGTTTTGATTTT	1	0.5586592178770949	No Hit
CTCCTCAGGAGTTATCCACAGGGTTATCCACCAACTGGGGACAACTCACATGGATGTAATTTCAACCAATG	1	0.5586592178770949	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGGCGGCGTGGGGTTGAAAAAAAAAAT	1	0.5586592178770949	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGAC	1	0.5586592178770949	No Hit
GCTCGAGGCCATGGGAGAACGTCAAGTCTCAGCAGATGGCCTGACTCATCCCCTCGAAGCCCCGTTCATGG	1	0.5586592178770949	No Hit
ATTCTGGCCTTCCCCGTTGAGACGGTGCATACCGCCGACCGCCCCGTGCAGCGCTTGGCCGAGATCGTGG	1	0.5586592178770949	No Hit
ATCTTTTCCCCACCTTTCCCCGCTTTCTATTCCACAAAACCACCATTGTCATCATGGCCCGTTCTCAATGA	1	0.5586592178770949	No Hit
TCCTAATTCCGTTTCAAAAAGACATCCTTTCAGTTCCTCACTCTGGCCATGGACATTTCCATGTGAAAATA	1	0.5586592178770949	No Hit
GGGGAATGCGAACACCGCCCACAGCAGGACGACCCAGCCCCAGCCGGGCACTTCCGCTGCGCGCAACCGCG	1	0.5586592178770949	No Hit
TGTCTACATTGTTCTCGAACCGTAGGATCCGCACGCTTAACGTTACGATGACAATTTCATTATGAGTTTAT	1	0.5586592178770949	No Hit
GGAGTGAGCCACTGGATGGCCAATCACCGCACAGCGCATCGCCAGTCCTCCTAGATCAGCACGTATTGTC	1	0.5586592178770949	No Hit
CTTCTGTACGGCATATTCTGCTGTTCCTACTCTGCCATAACAAAGGTATAATTATCACGACCAAGATATCG	1	0.5586592178770949	No Hit
GGTGATTCACCTACCTTGGTCTCCCAAAGTGTTGGGATTACAGGTGTGAGCCACACAAGGCTGGATTACAT	1	0.5586592178770949	No Hit
GGGTCGCCGGCTGCTGCGGCTGTCCATCGAGCTCAACAAGTCGGGTACCGCGGTGATCATCGCCACACATG	1	0.5586592178770949	No Hit
CAACTAAGGACGTCACCACCCTAGGGCGGGGGCGTCTGACACCACTGCGGTCGCCCTGGCCAGCGCCCTGG	1	0.5586592178770949	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.5586592178770949	No Hit
CATGTGCATCGCGCCACATCAAGCGGTGACGAGACAACGCTGTGCGAGAT	1	0.5586592178770949	No Hit
ATGCCGTATCGTGCGACGGCGCCGATGTCACCACCATTGAGGGGCTCGACGCCGACGAAATTGCTACTGAA	1	0.5586592178770949	No Hit
CAATGAAACTGAGGCTTAAAGAAGCTAAGGACTTTGGGAGACAGTGTGGTATAGAAGAATCGTGGACCTTG	1	0.5586592178770949	No Hit
GCATTCTTCTGCCTTGTACTCCAGATAGTATACAAAGAATCAAATTATAGTAGCTAAGTGGCATAGAATTG	1	0.5586592178770949	No Hit
CTTTTGTGCTGGCAAAATAAGACGCCTGCACCGATTTTTTACCCGCAAAAGCACGTAATGCTAATGCCGCA	1	0.5586592178770949	No Hit
GTCTCAGTGTGATTGATGGGTTTTATACACTGTCTTGAGCCTTTCCTGGGAAGGTGAATCCGTGACTTCC	1	0.5586592178770949	No Hit
CTCCAAGCCCTCTCCCAGAGACTACAGAGAACGTGGTGTGTACCCTGGGCCTGACTGTGGGGCGGGTGGG	1	0.5586592178770949	No Hit
CGCCACGCCATCGCCTATGCGAAATCGATCGGCACCGAGGCCGGCCTCGTCGGTCCGGCGTGTGCGCAGTT	1	0.5586592178770949	No Hit
GTGCTTGACCGCACCGCATTGGTCGACGCCACCGTGAAGACGGTCGCGACCAATCTCGGCGAAGGCGCGG	1	0.5586592178770949	No Hit
CCCCATGAGCCCACGACAACGCCTCGACGTCGTGGAAGGCTGGCTCCCATGTCACCCGGTGAGCCTCTTTG	1	0.5586592178770949	No Hit
GAAGGGGGTTTGAATAAGTTCCAGTTCAGATGTATATTTTCACATAGAAGTTATGAAATTGTTTTAAAGGA	1	0.5586592178770949	No Hit
ACTCTGCTTTCTGTGGTGTTGGCTTCATTCTCAGGCAGAGATCTCCAATTATGTCCACTGATGGTTTCAGG	1	0.5586592178770949	No Hit
GGCTGGAGTGCAGTGGCGCAATCTCAGCTTACGGCAAGCTCCGCCTCCCGGGTTCATGCCATTCTCCTGC	1	0.5586592178770949	No Hit
ACCTAAGATGGCAAGATATCATTGCCATCTTAGGTATGGAAGAATTGTCTGAAGCGGATAAATTGACAGTA	1	0.5586592178770949	No Hit
GAGAATGAATCAGGAGGCGAGAACCTCGGCGAGCATCTCCTCAGCCCGGTCATCAGCTACCGTTTTGGCGA	1	0.5586592178770949	No Hit
GATTATATATCAGTAAGAGCCCTGAGAGATGAACAGTTAGATAGGTTAGAAGTGGAAGTGTGGCGACACAT	1	0.5586592178770949	No Hit
GACCGTAATGACGCCGTTTTCGGTGTTCGGTGGTGCCACGTAATTCGATGCTTTGACAATGTTGGGATGG	1	0.5586592178770949	No Hit
CTCAGTGAGGTCATGCTACCCTCGACCAACGCTTTCTTGCAGGGTCCAGAGGCATACACGCATTTAAAGC	1	0.5586592178770949	No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA	1	0.5586592178770949	No Hit
CCTTTAATGAAGATGGCGATATCGGCCAGCAGCCGCCGAGCCCATGGCACCGGTCAGTTACCAGTGTGCCA	1	0.5586592178770949	No Hit
GTCTAAGTACGGACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG	1	0.5586592178770949	No Hit
CAACTCAACCGGACACTCGTGGAACATCGGCTTGCGTGAGGCGGCCGCGTGGTCGGTTAACACCTACTTTG	1	0.5586592178770949	No Hit
ACCCAGATCCGCTGGGAGTGCAGCTGGTGCTCTCAACACTGAGCCACCTCTCCAGGCCCCATGTCCCCCA	1	0.5586592178770949	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	1	0.5586592178770949	No Hit
GAAGGACTACGCCCGCACCGCGGCGCAGGACGAGTGGCCACAGATGATCGAGGGCGGCAGCGCCGCCACGA	1	0.5586592178770949	No Hit
GACCGGCGGTGCCGTGCCTCAGCCACCGAGGAGGAGCCGTGGCCCCGCCTCCCGTCACGACTGTGGGCCGA	1	0.5586592178770949	No Hit
GAGGTAACTGACTCCATGATTCAATTGGCTACAACTTGTTGCTCCCCTGACACACTGTCTCTTATAAACAA	1	0.5586592178770949	No Hit
TTCAAGACCCTGTCAGGCTGGAGAGAACTGGCTTTCTTATTCTCTCTCCCCTCC	1	0.5586592178770949	No Hit
GCCCTGGGCCGGGCCGGAAGTGCCGTTCAAGGCGGTGAGCTGGATGATGGACCGATACGGCCCGCTGGTGA	1	0.5586592178770949	No Hit
GTTTGTCACGCTGGACCACAGTGACGATGGTGGTCACGAGCTGCTTCTGCCTGGCCGTGAGGCCTCCGGCA	1	0.5586592178770949	No Hit
CAATAATATTCTGGAAAAGCCTAATTAGCGTCCTACCGATCTGGTTAAAGCTTCATCTCGAATCTGTCTCA	1	0.5586592178770949	No Hit
ATACCTAGGAATCCAACTTACAAGGAACATGAAGGACCTCTTCAAGGAGAACTACAAACCACTGATCAATG	1	0.5586592178770949	No Hit
TATTTTACCCTCCCGACTTCTTCGGGATTTTCGGTAAAAGCAAGATTGCGCAATTTCTTTGCTTTGGGTAG	1	0.5586592178770949	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTAA	1	0.5586592178770949	No Hit
AAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAACCGTCAATGTAGCAAAATA	1	0.5586592178770949	No Hit
GTTGATGATGGCGAAAACTTTTGCCGCGCGATAATAAATAGATGCTTTCTAATAGCGACGTTTTCCCG	1	0.5586592178770949	No Hit
GGTGACATCTTCCCCATCGATTACGACGCGGTCATCGATAGCTGAGGTATCAATCTCTAGGTGACAGCTTT	1	0.5586592178770949	No Hit
GCGCAAACGCTGCACAAGGTGCTGGAAATGTGCGGCGACAACCTGACCCGCGAGAACGTCATGAAACAGG	1	0.5586592178770949	No Hit
GTAGGCCTCTCTGAGCCTCATTTCTTGTCCGAACACCGAAGGCCCGAGCTCTGGGAGGCTCAAATCAACTA	1	0.5586592178770949	No Hit
GATGAATTCATACTACCTGAGATATCAAGCATCATCATGATATTTGGATTGCCAGGCGTGGGCAAGGCATA	1	0.5586592178770949	No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGTGTCGGGAGTGGGTAATTTGCGCGC	1	0.5586592178770949	No Hit
GACTAATTAAGTCAAGTTTGAATTGTCTTAAGGAATATTTTCACAATTGAGATGAATAAGGGGTAAGGCAA	1	0.5586592178770949	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCAATAC	1	0.5586592178770949	No Hit
CACGAGAATTGAGGGTAAATGCCATACTCCTCTTGTGGCCACAAAAGGAGTATAGGTGTGTCTATTATCAG	1	0.5586592178770949	No Hit
ACCTACAACCACCAGAAGCTAGAAGGCAGACACAGAACAGATGCTCCCTCAGAGACTCTAGAAAGAAAAAA	1	0.5586592178770949	No Hit
GTCCCAGGAGTCGACTTTGGCTTGAGGAAGGCAGGCGCCTTAGGTGGGGCAGTTGACCCACGCGCAGCGC	1	0.5586592178770949	No Hit
CTACCAGGAATCATGACCGGTCAAATCCTCGCTGGCCTGGACCCGATGGAGGCGGTGAAATATCAAATC	1	0.5586592178770949	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.5586592178770949	0.0
56	0.0	0.0	0.0	0.5586592178770949	0.0
57	0.0	0.0	0.0	0.5586592178770949	0.0
58	0.0	0.0	0.0	0.5586592178770949	0.0
59	0.0	0.0	0.0	0.5586592178770949	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 2 READS because READLEN < 1
Read 2 spots for SRR13210229.sra
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Written 2 spots for SRR13210229.sra
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Written 2 spots for SRR13210229.sra
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Rejected 53 READS because READLEN < 1
Read 53 spots for SRR13210229.sra
Written 53 spots for SRR13210229.sra
SRR ids: ['SRR13210229.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_pljmhw0y
SRR13210229.sra spots: 179
blocks: [[1, 2], [3, 4], [5, 6], [7, 8], [9, 10], [11, 12], [13, 14], [15, 16], [17, 18], [19, 20], [21, 22], [23, 24], [25, 26], [27, 28], [29, 30], [31, 32], [33, 34], [35, 36], [37, 38], [39, 40], [41, 42], [43, 44], [45, 46], [47, 48], [49, 50], [51, 52], [53, 54], [55, 56], [57, 58], [59, 60], [61, 62], [63, 64], [65, 66], [67, 68], [69, 70], [71, 72], [73, 74], [75, 76], [77, 78], [79, 80], [81, 82], [83, 84], [85, 86], [87, 88], [89, 90], [91, 92], [93, 94], [95, 96], [97, 98], [99, 100], [101, 102], [103, 104], [105, 106], [107, 108], [109, 110], [111, 112], [113, 114], [115, 116], [117, 118], [119, 120], [121, 122], [123, 124], [125, 126], [127, 179]]
SRR13210229 file size 32
SRR13210229 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13210229 SRR13210229_1.fastq
Input file:	SRR13210229_1.fastq
trimmed:	SRR13210229-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri May  6 15:03:29 2022 >> started

Fri May  6 15:03:29 2022 >> done (0.002s)
179 reads processed; of these:
  0 ( 0.00%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
179 (100.00%) reads available; of these:
179 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 41	  1	  0.56%
 42	  0	  0.00%
 43	  1	  0.56%
 44	  1	  0.56%
 45	  0	  0.00%
 46	  1	  0.56%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  2	  1.12%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  2	  1.12%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  1	  0.56%
 68	  5	  2.79%
 69	 16	  8.94%
 70	 45	 25.14%
 71	104	 58.10%
179 reads passed initial QC
                                 Started job on |	May 06 15:03:52
                             Started mapping on |	May 06 15:03:52
                                    Finished on |	May 06 15:03:56
       Mapping speed, Million of reads per hour |	0.16

                          Number of input reads |	179
                      Average input read length |	69
                                    UNIQUE READS:
                   Uniquely mapped reads number |	52
                        Uniquely mapped reads % |	29.05%
                          Average mapped length |	68.21
                       Number of splices: Total |	0
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	0
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	0.73%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	25
             % of reads mapped to multiple loci |	13.97%
        Number of reads mapped to too many loci |	4
             % of reads mapped to too many loci |	2.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	54.75%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	102	102	102
N_multimapping	25	25	25
N_noFeature	45	47	50
N_ambiguous	0	0	0
UnstrandedReadsAssigned:7 PositiveStrandReadsAssigned:5 NegativeStrandReadsAssigned:2
Dataset is classified unstranded
MeadianReadLen=71 20thPercentileLength=70 echo kmer=65
SRR13210229 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 180,869
[index] number of k-mers: 106,891,614
[index] number of equivalence classes: 723,554
[quant] running in single-end mode
[quant] will process file 1: SRR13210229-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 179 reads, 16 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 243 rounds

 180870 SRR13210229.ke.tsv
  58302 SRR13210229.se.tsv
 239172 total
==> SRR13210229.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENST00000434970.2	9	3.70903	0	0
ENST00000448914.1	13	4.28456	0	0
ENST00000415118.1	8	3.52171	0	0
ENST00000631435.1	12	4.16231	0	0
ENST00000632684.1	12	4.16231	0	0
ENST00000454908.1	17	4.67723	0	0
ENST00000390567.1	20	4.90697	0	0
ENST00000603326.1	19	4.8345	0	0
ENST00000414852.1	16	4.59059	0	0

==> SRR13210229.se.tsv <==
ENSG00000223972	0
ENSG00000227232	0
ENSG00000278267	0
ENSG00000243485	0
ENSG00000284332	0
ENSG00000237613	0
ENSG00000268020	0
ENSG00000240361	0
ENSG00000186092	0
ENSG00000238009	0
SRR13210229 completed mapping pipeline successfully
