Starting /dee2/code/volunteer_pipeline.sh SRR5030889
    current disk space = 160641269760
    free memory = 73912592 
SRR5030889 SRAfilesize
72d9cf7bae7aac17d6aa66a16bb88558  SRR5030889.sra
SRR5030889.sra file validated
SRR5030889 is single end
SRR5030889 is conventional basespace
SRR5030889 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.502	34.0	31.0	34.0	31.0	34.0
2	32.7695	34.0	31.0	34.0	31.0	34.0
3	32.8125	34.0	31.0	34.0	31.0	34.0
4	36.24975	37.0	37.0	37.0	35.0	37.0
5	36.3395	37.0	37.0	37.0	35.0	37.0
6	36.134	37.0	36.0	37.0	35.0	37.0
7	36.10125	37.0	35.0	37.0	35.0	37.0
8	36.23	37.0	36.0	37.0	35.0	37.0
9	37.95275	39.0	38.0	39.0	35.0	39.0
10	38.02575	39.0	39.0	39.0	35.0	39.0
11	38.00675	39.0	38.0	39.0	35.0	39.0
12	37.989	39.0	38.0	39.0	35.0	39.0
13	37.8945	39.0	38.0	39.0	35.0	39.0
14	39.3175	41.0	39.0	41.0	36.0	41.0
15	39.2565	41.0	39.0	41.0	36.0	41.0
16	39.208	40.0	39.0	41.0	36.0	41.0
17	39.39525	41.0	39.0	41.0	36.0	41.0
18	39.35275	41.0	39.0	41.0	36.0	41.0
19	39.186	40.0	39.0	41.0	36.0	41.0
20	39.14825	40.0	39.0	41.0	36.0	41.0
21	39.25425	40.0	39.0	41.0	36.0	41.0
22	39.19125	41.0	39.0	41.0	36.0	41.0
23	39.012	40.0	39.0	41.0	35.0	41.0
24	38.71375	40.0	38.0	41.0	35.0	41.0
25	39.07525	41.0	39.0	41.0	36.0	41.0
26	39.08175	41.0	39.0	41.0	36.0	41.0
27	39.15075	41.0	39.0	41.0	36.0	41.0
28	38.9525	40.0	39.0	41.0	35.0	41.0
29	38.2465	40.0	38.0	41.0	33.0	41.0
30	38.37975	40.0	38.0	41.0	34.0	41.0
31	38.735	40.0	38.0	41.0	35.0	41.0
32	38.937	40.0	38.0	41.0	35.0	41.0
33	37.863	40.0	38.0	41.0	32.0	41.0
34	38.08475	40.0	38.0	41.0	33.0	41.0
35	37.874	40.0	38.0	41.0	33.0	41.0
36	34.36475	38.0	31.0	40.0	23.0	41.0
37	35.601	38.0	34.0	40.0	29.0	41.0
38	36.999	39.0	36.0	40.0	32.0	41.0
39	37.04525	39.0	37.0	40.0	31.0	41.0
40	37.08275	39.0	37.0	40.0	31.0	41.0
41	37.02675	39.0	36.0	41.0	31.0	41.0
42	37.224	40.0	37.0	41.0	32.0	41.0
43	37.016	40.0	37.0	41.0	31.0	41.0
44	36.9735	39.0	37.0	41.0	31.0	41.0
45	36.68925	39.0	36.0	41.0	31.0	41.0
46	34.8875	38.0	34.0	40.0	25.0	41.0
47	35.235	38.0	34.0	40.0	27.0	41.0
48	35.25325	39.0	35.0	40.0	28.0	41.0
49	34.475	38.0	34.0	40.0	26.0	41.0
50	30.0325	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	1.0
17	1.0
18	1.0
19	3.0
20	7.0
21	5.0
22	3.0
23	8.0
24	15.0
25	14.0
26	19.0
27	23.0
28	38.0
29	37.0
30	44.0
31	78.0
32	90.0
33	117.0
34	150.0
35	176.0
36	300.0
37	549.0
38	1038.0
39	1281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.680840420210105	23.06153076538269	47.34867433716858	17.90895447723862
2	23.95	24.4	19.575	32.074999999999996
3	25.025	18.099999999999998	21.05	35.825
4	32.475	25.6	14.625	27.3
5	20.724999999999998	42.375	23.75	13.15
6	31.6	15.174999999999999	36.425000000000004	16.8
7	17.299999999999997	20.4	24.675	37.625
8	25.224999999999998	28.549999999999997	17.625	28.599999999999998
9	22.5	31.8	29.325000000000003	16.375
10	22.475	24.0	30.225	23.3
11	31.724999999999998	14.95	15.575	37.75
12	15.925	20.674999999999997	30.7	32.7
13	29.375	19.650000000000002	31.574999999999996	19.400000000000002
14	24.775	32.15	22.225	20.849999999999998
15	32.70817704426106	26.85671417854464	24.456114028507127	15.978994748687173
16	21.0	22.925	20.474999999999998	35.6
17	21.75	25.0	38.3	14.95
18	21.224999999999998	18.975	32.5	27.3
19	39.300000000000004	14.725	26.75	19.225
20	40.875	15.825	29.15	14.149999999999999
21	25.95	18.5	27.05	28.499999999999996
22	28.675	14.825	45.45	11.05
23	26.974999999999998	14.274999999999999	41.625	17.125
24	49.875	16.3	23.225	10.6
25	45.7	26.125	15.049999999999999	13.125
26	24.75	44.224999999999994	15.775	15.25
27	16.05	44.525	23.400000000000002	16.025
28	13.625000000000002	22.85	45.175	18.35
29	14.575	13.575000000000001	53.825	18.025
30	11.600000000000001	9.025	33.0	46.375
31	16.0	7.1	47.199999999999996	29.7
32	30.099999999999998	8.175	20.175	41.55
33	52.125	11.425	13.100000000000001	23.35
34	56.025000000000006	9.35	18.525	16.1
35	60.224999999999994	9.075	18.275	12.425
36	42.175000000000004	10.174999999999999	33.475	14.174999999999999
37	43.85	12.775	20.075000000000003	23.3
38	23.35	18.85	12.325	45.475
39	16.675	25.525	11.975	45.824999999999996
40	17.349999999999998	46.75	14.274999999999999	21.625
41	25.05	45.475	11.025	18.45
42	46.825	27.750000000000004	14.6	10.825
43	46.550000000000004	29.975	11.625	11.85
44	27.800000000000004	47.775	8.375	16.05
45	20.575	48.325	13.3	17.8
46	14.475	27.025	19.0	39.5
47	10.85	16.475	40.425	32.25
48	13.775	13.875000000000002	20.424999999999997	51.925
49	16.5	20.1	13.825000000000001	49.575
50	19.5	35.075	14.549999999999999	30.875000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.5
28	2.0
29	4.0
30	6.0
31	18.0
32	30.0
33	30.5
34	31.0
35	43.0
36	55.0
37	62.5
38	70.0
39	67.5
40	65.0
41	56.5
42	48.0
43	92.0
44	136.0
45	168.5
46	201.0
47	303.0
48	405.0
49	477.5
50	550.0
51	634.0
52	718.0
53	572.5
54	427.0
55	477.0
56	527.0
57	419.5
58	312.0
59	213.0
60	114.0
61	77.5
62	41.0
63	99.5
64	158.0
65	99.0
66	40.0
67	28.0
68	16.0
69	13.0
70	10.0
71	14.5
72	19.0
73	14.0
74	9.0
75	5.0
76	1.0
77	1.5
78	2.0
79	2.0
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.550000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89395807644883	30.775000000000002
2	9.3711467324291	7.6
3	4.5006165228113435	5.475
4	1.726263871763255	2.8000000000000003
5	1.8495684340320593	3.75
6	0.9864364981504316	2.4
7	1.0480887792848335	2.9749999999999996
8	0.8014796547472256	2.6
9	0.12330456226880394	0.44999999999999996
>10	3.1442663378545004	22.425
>50	0.3082614056720099	7.675
>100	0.2466091245376079	11.075
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	117	2.9250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	111	2.775	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	109	2.725	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	106	2.65	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	78	1.95	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	57	1.425	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	53	1.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	51	1.275	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	27	0.675	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC	24	0.6	RNA PCR Primer, Index 11 (100% over 44bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC	24	0.6	RNA PCR Primer, Index 11 (100% over 50bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG	22	0.5499999999999999	RNA PCR Primer, Index 2 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	19	0.475	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	18	0.44999999999999996	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	15	0.375	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	15	0.375	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	15	0.375	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	14	0.35000000000000003	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	14	0.35000000000000003	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	11	0.27499999999999997	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 32bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	10	0.25	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	10	0.25	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	9	0.22499999999999998	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	9	0.22499999999999998	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	8	0.2	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	8	0.2	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTTGGAATTCTC	7	0.17500000000000002	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	7	0.17500000000000002	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	6	0.15	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTAT	6	0.15	RNA PCR Primer, Index 11 (100% over 48bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGCATTCTCGGGTGCC	5	0.125	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	5	0.125	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	5	0.125	No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACTGCTAATGAAAACACAG	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GAGCAATGATGACCCCAACATGCCATCTGAGTGGCTTTGCTGAAATCCAG	5	0.125	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGACACTCTGTGCTGAATGTC	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.625	0.0	0.0	0.0
2	0.0	0.625	0.0	0.0	0.0
3	0.0	0.825	0.0	0.0	0.0
4	0.0	0.85	0.0	0.0	0.0
5	0.0	0.925	0.0	0.0	0.0
6	0.0	0.975	0.0	0.0	0.0
7	0.0	1.8	0.0	0.0	0.0
8	0.0	2.0	0.0	0.0	0.0
9	0.0	2.05	0.0	0.0	0.0
10	0.0	2.125	0.0	0.0	0.0
11	0.0	2.275	0.0	0.0	0.0
12	0.0	2.575	0.0	0.0	0.0
13	0.0	2.775	0.0	0.0	0.0
14	0.0	2.925	0.0	0.0	0.0
15	0.0	3.3	0.0	0.0	0.0
16	0.0	4.3	0.0	0.0	0.0
17	0.0	4.975	0.0	0.0	0.0
18	0.0	6.55	0.0	0.0	0.0
19	0.0	7.475	0.0	0.0	0.0
20	0.0	8.375	0.0	0.0	0.0
21	0.0	13.85	0.0	0.0	0.0
22	0.0	24.1	0.0	0.0	0.0
23	0.0	51.25	0.0	0.0	0.0
24	0.0	61.675	0.0	0.0	0.0
25	0.0	66.575	0.0	0.0	0.0
26	0.0	68.025	0.0	0.0	0.0
27	0.0	68.575	0.0	0.0	0.0
28	0.0	69.15	0.0	0.0	0.0
29	0.0	69.65	0.0	0.0	0.0
30	0.0	69.975	0.0	0.0	0.0
31	0.0	70.675	0.0	0.0	0.0
32	0.0	71.3	0.0	0.0	0.0
33	0.0	72.025	0.0	0.0	0.0
34	0.0	75.7	0.0	0.0	0.0
35	0.0	76.825	0.0	0.0	0.0
36	0.0	77.5	0.0	0.0	0.0
37	0.0	78.55	0.0	0.0	0.0
38	0.0	78.925	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
ACGCTGT	20	6.952051E-4	44.000004	9
CTGAGAC	20	6.952051E-4	44.000004	4
TTCAACG	20	6.952051E-4	44.000004	5
TCGGTGA	20	6.952051E-4	44.000004	15
CCTGAGA	20	6.952051E-4	44.000004	3
CATTCAA	20	6.952051E-4	44.000004	3
TCAACGC	20	6.952051E-4	44.000004	6
ACATTCA	20	6.952051E-4	44.000004	2
GCTGTCG	20	6.952051E-4	44.000004	11
CCCTGAG	20	6.952051E-4	44.000004	2
CTGTCGG	20	6.952051E-4	44.000004	12
ATTCAAC	20	6.952051E-4	44.000004	4
GTCGGTG	20	6.952051E-4	44.000004	14
AACATTC	20	6.952051E-4	44.000004	1
TGTCGGT	20	6.952051E-4	44.000004	13
TCCCTGA	20	6.952051E-4	44.000004	1
CAACGCT	20	6.952051E-4	44.000004	7
CGCTGTC	20	6.952051E-4	44.000004	10
GAGGTAG	25	3.6958358E-5	44.0	2
>>END_MODULE
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173108 spots for SRR5030889.sra
Written 173108 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
Read 173107 spots for SRR5030889.sra
Written 173107 spots for SRR5030889.sra
SRR ids: ['SRR5030889.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9aut1pk7
SRR5030889.sra spots: 2077285
blocks: [[1, 173107], [173108, 346214], [346215, 519321], [519322, 692428], [692429, 865535], [865536, 1038642], [1038643, 1211749], [1211750, 1384856], [1384857, 1557963], [1557964, 1731070], [1731071, 1904177], [1904178, 2077285]]
SRR5030889 file size 358960
SRR5030889 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030889 SRR5030889_1.fastq
Input file:	SRR5030889_1.fastq
trimmed:	SRR5030889-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:37:26 2020 >> started

Wed Mar  4 06:37:29 2020 >> done (2.599s)
2077285 reads processed; of these:
     77 ( 0.00%) short reads filtered out after trimming by size control
      1 ( 0.00%) empty reads filtered out after trimming by size control
2077207 (100.00%) reads available; of these:
 129042 ( 6.21%) trimmed reads available after processing
1948165 (93.79%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     23	  0.00%
 19	     32	  0.00%
 20	     96	  0.00%
 21	     88	  0.00%
 22	    170	  0.01%
 23	    199	  0.01%
 24	    259	  0.01%
 25	    302	  0.01%
 26	    281	  0.01%
 27	    334	  0.02%
 28	    470	  0.02%
 29	    584	  0.03%
 30	    490	  0.02%
 31	    633	  0.03%
 32	    714	  0.03%
 33	    858	  0.04%
 34	   1893	  0.09%
 35	   1676	  0.08%
 36	   1367	  0.07%
 37	   1491	  0.07%
 38	   1704	  0.08%
 39	   2087	  0.10%
 40	   2280	  0.11%
 41	   2424	  0.12%
 42	   3420	  0.16%
 43	   4067	  0.20%
 44	   7239	  0.35%
 45	  10272	  0.49%
 46	   9696	  0.47%
 47	  13462	  0.65%
 48	  25614	  1.23%
 49	  34817	  1.68%
 50	1948165	 93.79%
2077207 reads passed initial QC


criterion=sequence-density
sequence-density=79.45
sequence-density-rank=1
fanout-score=18.69
fanout-score-rank=1
prefix-density=81.13
prefix-fanout=18.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC


criterion=fanout-score
sequence-density=79.45
sequence-density-rank=1
fanout-score=18.69
fanout-score-rank=1
prefix-density=81.13
prefix-fanout=18.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC -o SRR5030889 -
Input file:	STDIN
trimmed:	SRR5030889-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:37:50 2020 >> started

Wed Mar  4 06:37:55 2020 >> done (5.640s)
2025277 reads processed; of these:
 126160 ( 6.23%) short reads filtered out after trimming by size control
  13783 ( 0.68%) empty reads filtered out after trimming by size control
1885334 (93.09%) reads available; of these:
1651937 (87.62%) trimmed reads available after processing
 233397 (12.38%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  17092	  0.91%
 19	  18356	  0.97%
 20	 100506	  5.33%
 21	 206599	 10.96%
 22	 549889	 29.17%
 23	 225721	 11.97%
 24	 106477	  5.65%
 25	  29775	  1.58%
 26	  15016	  0.80%
 27	  12525	  0.66%
 28	  13959	  0.74%
 29	  10308	  0.55%
 30	  13790	  0.73%
 31	  15808	  0.84%
 32	  18311	  0.97%
 33	  84554	  4.48%
 34	  21520	  1.14%
 35	  16710	  0.89%
 36	  18821	  1.00%
 37	  10166	  0.54%
 38	  10706	  0.57%
 39	  12063	  0.64%
 40	  16854	  0.89%
 41	  21339	  1.13%
 42	  25037	  1.33%
 43	  18201	  0.97%
 44	  14772	  0.78%
 45	  16826	  0.89%
 46	  13049	  0.69%
 47	  11511	  0.61%
 48	   2513	  0.13%
 49	   8708	  0.46%
 50	 207852	 11.02%


criterion=sequence-density
sequence-density=4.54
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCTTTAACCTGTGA


criterion=fanout-score
sequence-density=2.20
sequence-density-rank=4
fanout-score=18.69
fanout-score-rank=1
prefix-density=2.26
prefix-fanout=18.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC
                                 Started job on |	Mar 04 06:38:25
                             Started mapping on |	Mar 04 06:38:26
                                    Finished on |	Mar 04 06:38:49
       Mapping speed, Million of reads per hour |	303.22

                          Number of input reads |	1937264
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1088059
                        Uniquely mapped reads % |	56.16%
                          Average mapped length |	27.92
                       Number of splices: Total |	797
            Number of splices: Annotated (sjdb) |	138
                       Number of splices: GT/AG |	740
                       Number of splices: GC/AG |	14
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	43
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.50
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	580653
             % of reads mapped to multiple loci |	29.97%
        Number of reads mapped to too many loci |	49776
             % of reads mapped to too many loci |	2.57%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.71%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	268552	268552	268552
N_multimapping	580653	580653	580653
N_noFeature	48522	61033	770608
N_ambiguous	618774	349418	11979
UnstrandedReadsAssigned:420763 PositiveStrandReadsAssigned:677608 NegativeStrandReadsAssigned:305472
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030889 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030889-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,937,264 reads, 516,848 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,097 rounds

 109283 SRR5030889.ke.tsv
  52636 SRR5030889.se.tsv
 161919 total
==> SRR5030889.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030889.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030889 completed mapping pipeline successfully
