Starting /dee2/code/volunteer_pipeline.sh SRR5031018
    current disk space = 160547684352
    free memory = 73952448 
SRR5031018 SRAfilesize
fd67b11bd5267694bd0eed6bfe5a4716  SRR5031018.sra
SRR5031018.sra file validated
SRR5031018 is single end
SRR5031018 is conventional basespace
SRR5031018 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.299	33.0	31.0	34.0	30.0	34.0
2	32.56475	34.0	31.0	34.0	31.0	34.0
3	32.6235	34.0	31.0	34.0	31.0	34.0
4	36.145	37.0	35.0	37.0	35.0	37.0
5	36.046	37.0	35.0	37.0	35.0	37.0
6	35.86925	37.0	35.0	37.0	35.0	37.0
7	35.88175	37.0	35.0	37.0	35.0	37.0
8	35.97325	37.0	35.0	37.0	35.0	37.0
9	37.612	39.0	37.0	39.0	35.0	39.0
10	37.426	39.0	37.0	39.0	34.0	39.0
11	37.15	39.0	37.0	39.0	33.0	39.0
12	37.56475	39.0	37.0	39.0	35.0	39.0
13	37.56075	39.0	37.0	39.0	35.0	39.0
14	38.91575	40.0	38.0	41.0	35.0	41.0
15	38.8625	40.0	38.0	41.0	35.0	41.0
16	38.869	40.0	38.0	41.0	35.0	41.0
17	39.0505	40.0	38.0	41.0	35.0	41.0
18	38.948	41.0	38.0	41.0	35.0	41.0
19	38.8075	40.0	38.0	41.0	34.0	41.0
20	38.6875	40.0	38.0	41.0	34.0	41.0
21	38.79325	40.0	38.0	41.0	34.0	41.0
22	38.695	40.0	38.0	41.0	34.0	41.0
23	38.46125	40.0	38.0	41.0	34.0	41.0
24	38.832	40.0	38.0	41.0	34.0	41.0
25	38.90525	40.0	38.0	41.0	35.0	41.0
26	38.79075	40.0	38.0	41.0	34.0	41.0
27	38.87925	40.0	38.0	41.0	35.0	41.0
28	38.62375	40.0	38.0	41.0	34.0	41.0
29	38.37825	40.0	38.0	41.0	34.0	41.0
30	38.3275	40.0	38.0	41.0	33.0	41.0
31	38.4365	40.0	38.0	41.0	34.0	41.0
32	38.53025	40.0	38.0	41.0	34.0	41.0
33	38.214	40.0	38.0	41.0	33.0	41.0
34	38.0485	40.0	38.0	41.0	33.0	41.0
35	37.9235	40.0	38.0	41.0	33.0	41.0
36	37.812	40.0	37.0	41.0	33.0	41.0
37	37.13625	40.0	37.0	41.0	31.0	41.0
38	37.0665	39.0	36.0	41.0	31.0	41.0
39	37.66325	39.0	37.0	41.0	33.0	41.0
40	37.72125	40.0	37.0	41.0	33.0	41.0
41	37.86175	40.0	37.0	41.0	33.0	41.0
42	37.86025	40.0	37.0	41.0	33.0	41.0
43	37.6805	40.0	37.0	41.0	33.0	41.0
44	37.87225	40.0	37.0	41.0	33.0	41.0
45	37.8295	40.0	37.0	41.0	33.0	41.0
46	37.5425	40.0	37.0	41.0	33.0	41.0
47	36.84325	40.0	36.0	41.0	31.0	41.0
48	36.6145	39.0	35.0	41.0	30.0	41.0
49	36.79675	40.0	36.0	41.0	31.0	41.0
50	36.05525	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	3.0
18	1.0
19	4.0
20	2.0
21	5.0
22	2.0
23	8.0
24	6.0
25	9.0
26	15.0
27	21.0
28	32.0
29	40.0
30	64.0
31	60.0
32	77.0
33	118.0
34	158.0
35	206.0
36	238.0
37	433.0
38	764.0
39	1732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.025	25.525	47.8	16.650000000000002
2	22.915101427498122	27.798647633358375	19.108439769596792	30.177811169546708
3	24.975	17.5	23.849999999999998	33.675
4	35.575	28.875	14.499999999999998	21.05
5	21.525	37.824999999999996	26.55	14.099999999999998
6	28.249999999999996	16.3	39.825	15.625
7	18.175	20.325	20.5	41.0
8	21.349999999999998	28.549999999999997	17.575	32.525
9	26.0	30.65	26.575	16.775000000000002
10	22.325	19.125	30.049999999999997	28.499999999999996
11	32.300000000000004	12.525	17.549999999999997	37.625
12	17.275	18.95	33.475	30.3
13	29.15	16.775000000000002	32.574999999999996	21.5
14	29.299999999999997	31.275	20.175	19.25
15	38.074999999999996	22.375	24.8	14.75
16	18.099999999999998	18.7	25.2	38.0
17	27.075	22.15	36.75	14.025000000000002
18	27.175	16.6	31.175000000000004	25.05
19	37.525	14.825	30.8	16.85
20	45.2	16.675	22.975	15.15
21	23.799999999999997	22.625	24.275	29.299999999999997
22	30.2	14.7	44.525	10.575
23	26.424999999999997	14.025000000000002	42.775	16.775000000000002
24	49.35	15.425	25.575	9.65
25	48.375	24.725	15.825	11.075
26	27.625	44.6	15.45	12.325
27	16.425	44.45	24.425	14.7
28	12.625	23.849999999999998	46.875	16.650000000000002
29	13.475000000000001	12.775	54.225	19.525000000000002
30	11.200000000000001	7.225	35.275	46.300000000000004
31	15.35	5.5	45.125	34.025
32	28.575	6.800000000000001	22.55	42.075
33	52.6	8.924999999999999	13.950000000000001	24.525
34	58.074999999999996	8.575000000000001	16.650000000000002	16.7
35	61.824999999999996	8.025	19.650000000000002	10.5
36	41.225	8.975	35.525	14.274999999999999
37	45.125	10.9	20.150000000000002	23.825
38	24.4	17.5	12.075	46.025
39	16.725	24.05	11.799999999999999	47.425
40	16.425	47.075	11.275	25.224999999999998
41	24.6	46.325	10.925	18.15
42	46.225	29.525000000000002	12.9	11.35
43	49.5	28.999999999999996	9.55	11.95
44	30.075000000000003	45.800000000000004	8.85	15.275
45	19.875	46.525	14.7	18.9
46	15.625	24.2	18.25	41.925000000000004
47	10.725	15.75	37.85	35.675000000000004
48	12.174999999999999	13.725000000000001	20.0	54.1
49	14.35	18.95	15.475	51.225
50	18.375	35.275	15.625	30.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.0
28	1.0
29	1.5
30	2.0
31	10.0
32	18.0
33	15.5
34	13.0
35	16.5
36	20.0
37	30.5
38	41.0
39	41.5
40	42.0
41	46.0
42	50.0
43	79.0
44	108.0
45	129.0
46	150.0
47	291.5
48	433.0
49	526.5
50	620.0
51	707.5
52	795.0
53	598.0
54	401.0
55	504.0
56	607.0
57	452.5
58	298.0
59	192.0
60	86.0
61	62.5
62	39.0
63	75.0
64	111.0
65	70.0
66	29.0
67	23.0
68	17.0
69	24.0
70	31.0
71	51.5
72	72.0
73	39.5
74	7.0
75	4.5
76	2.0
77	1.0
78	0.0
79	1.0
80	2.0
81	1.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08854166666666	28.449999999999996
2	11.328125	8.7
3	4.1015625	4.725
4	2.018229166666667	3.1
5	1.953125	3.75
6	0.4557291666666667	1.05
7	0.9114583333333334	2.45
8	0.8463541666666666	2.6
9	0.5859375	2.025
>10	3.125	21.099999999999998
>50	0.32552083333333337	9.3
>100	0.26041666666666663	12.75
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	169	4.2250000000000005	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	124	3.1	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	102	2.55	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	100	2.5	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	90	2.25	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	63	1.575	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	57	1.425	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	34	0.8500000000000001	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	31	0.775	RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	27	0.675	RNA PCR Primer, Index 1 (97% over 35bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGC	24	0.6	RNA PCR Primer, Index 38 (100% over 50bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	19	0.475	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTC	17	0.42500000000000004	RNA PCR Primer, Index 38 (100% over 44bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	16	0.4	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	12	0.3	No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	12	0.3	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	11	0.27499999999999997	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	10	0.25	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	7	0.17500000000000002	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	7	0.17500000000000002	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	7	0.17500000000000002	RNA PCR Primer, Index 2 (100% over 34bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT	6	0.15	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGCC	5	0.125	No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	5	0.125	RNA PCR Primer, Index 1 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGCATTCTCGGGTGCC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CTTTTTGCGGTCTGGGCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AAGAAAGATTGCAAGAACTGCTAATTCATGCTTACCTGGAATTCTCGGGT	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTAT	5	0.125	RNA PCR Primer, Index 38 (100% over 48bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.625	0.0	0.0	0.0
2	0.0	0.625	0.0	0.0	0.0
3	0.0	0.775	0.0	0.0	0.0
4	0.0	0.775	0.0	0.0	0.0
5	0.0	0.85	0.0	0.0	0.0
6	0.0	0.875	0.0	0.0	0.0
7	0.0	1.475	0.0	0.0	0.0
8	0.0	1.675	0.0	0.0	0.0
9	0.0	1.8	0.0	0.0	0.0
10	0.0	1.975	0.0	0.0	0.0
11	0.0	2.275	0.0	0.0	0.0
12	0.0	2.45	0.0	0.0	0.0
13	0.0	2.6	0.0	0.0	0.0
14	0.0	2.875	0.0	0.0	0.0
15	0.0	3.275	0.0	0.0	0.0
16	0.0	4.575	0.0	0.0	0.0
17	0.0	5.425	0.0	0.0	0.0
18	0.0	6.675	0.0	0.0	0.0
19	0.0	7.175	0.0	0.0	0.0
20	0.0	8.35	0.0	0.0	0.0
21	0.0	13.9	0.0	0.0	0.0
22	0.0	24.875	0.0	0.0	0.0
23	0.0	52.5	0.0	0.0	0.0
24	0.0	64.625	0.0	0.0	0.0
25	0.0	70.4	0.0	0.0	0.0
26	0.0	72.225	0.0	0.0	0.0
27	0.0	72.9	0.0	0.0	0.0
28	0.0	73.45	0.0	0.0	0.0
29	0.0	74.2	0.0	0.0	0.0
30	0.0	74.625	0.0	0.0	0.0
31	0.0	75.7	0.0	0.0	0.0
32	0.0	76.825	0.0	0.0	0.0
33	0.0	77.9	0.0	0.0	0.0
34	0.0	81.075	0.0	0.0	0.0
35	0.0	82.525	0.0	0.0	0.0
36	0.0	83.75	0.0	0.0	0.0
37	0.0	85.125	0.0	0.0	0.0
38	0.0	85.7	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.952051E-4	44.000004	2
GAGCTTG	40	5.315087E-9	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
TCGGATC	40	5.315087E-9	44.000004	1
CGGATCC	40	5.315087E-9	44.000004	2
CTTGGTG	20	6.952051E-4	44.000004	16
GATCCGT	40	5.315087E-9	44.000004	4
GGTAGTA	20	6.952051E-4	44.000004	4
GCTTGGT	20	6.952051E-4	44.000004	15
GCTTGGC	20	6.952051E-4	44.000004	15
TGAGGTA	20	6.952051E-4	44.000004	1
TCCGTCT	40	5.315087E-9	44.000004	6
GGTGGAA	20	6.952051E-4	44.000004	19
ATCCGTC	40	5.315087E-9	44.000004	5
TGGTGGA	20	6.952051E-4	44.000004	18
CGTCTGA	40	5.315087E-9	44.000004	8
GTAGTAG	20	6.952051E-4	44.000004	5
ACTGTTG	25	3.6958358E-5	44.0	18
GTTGAAG	25	3.6958358E-5	44.0	10
>>END_MODULE
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291504 spots for SRR5031018.sra
Written 291504 spots for SRR5031018.sra
Read 291515 spots for SRR5031018.sra
Written 291515 spots for SRR5031018.sra
SRR ids: ['SRR5031018.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_m7o2lwzc
SRR5031018.sra spots: 3498059
blocks: [[1, 291504], [291505, 583008], [583009, 874512], [874513, 1166016], [1166017, 1457520], [1457521, 1749024], [1749025, 2040528], [2040529, 2332032], [2332033, 2623536], [2623537, 2915040], [2915041, 3206544], [3206545, 3498059]]
SRR5031018 file size 605226
SRR5031018 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031018 SRR5031018_1.fastq
Input file:	SRR5031018_1.fastq
trimmed:	SRR5031018-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:07:18 2020 >> started

Wed Mar  4 07:07:20 2020 >> done (2.135s)
3498059 reads processed; of these:
    317 ( 0.01%) short reads filtered out after trimming by size control
      9 ( 0.00%) empty reads filtered out after trimming by size control
3497733 (99.99%) reads available; of these:
 213541 ( 6.11%) trimmed reads available after processing
3284192 (93.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    114	  0.00%
 19	    100	  0.00%
 20	    219	  0.01%
 21	    268	  0.01%
 22	    461	  0.01%
 23	    572	  0.02%
 24	    540	  0.02%
 25	    711	  0.02%
 26	    669	  0.02%
 27	    857	  0.02%
 28	    961	  0.03%
 29	   1732	  0.05%
 30	   1099	  0.03%
 31	   1118	  0.03%
 32	   1262	  0.04%
 33	   1720	  0.05%
 34	   1868	  0.05%
 35	   3636	  0.10%
 36	   3052	  0.09%
 37	   3468	  0.10%
 38	   3487	  0.10%
 39	   3675	  0.11%
 40	   3958	  0.11%
 41	   4299	  0.12%
 42	   5834	  0.17%
 43	   8120	  0.23%
 44	  11447	  0.33%
 45	  14823	  0.42%
 46	  21102	  0.60%
 47	  25453	  0.73%
 48	  38381	  1.10%
 49	  48535	  1.39%
 50	3284192	 93.89%
3497733 reads passed initial QC


criterion=sequence-density
sequence-density=85.02
sequence-density-rank=1
fanout-score=20.57
fanout-score-rank=1
prefix-density=87.19
prefix-fanout=20.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC


criterion=fanout-score
sequence-density=85.02
sequence-density-rank=1
fanout-score=20.57
fanout-score-rank=1
prefix-density=87.19
prefix-fanout=20.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC -o SRR5031018 -
Input file:	STDIN
trimmed:	SRR5031018-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:07:41 2020 >> started

Wed Mar  4 07:07:46 2020 >> done (4.558s)
3416390 reads processed; of these:
 242974 ( 7.11%) short reads filtered out after trimming by size control
  23680 ( 0.69%) empty reads filtered out after trimming by size control
3149736 (92.19%) reads available; of these:
2989640 (94.92%) trimmed reads available after processing
 160096 ( 5.08%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  18307	  0.58%
 19	  34457	  1.09%
 20	 184960	  5.87%
 21	 352098	 11.18%
 22	 940074	 29.85%
 23	 425286	 13.50%
 24	 203055	  6.45%
 25	  58868	  1.87%
 26	  30023	  0.95%
 27	  24417	  0.78%
 28	  26113	  0.83%
 29	  20140	  0.64%
 30	  29287	  0.93%
 31	  45891	  1.46%
 32	  40082	  1.27%
 33	 115557	  3.67%
 34	  47672	  1.51%
 35	  39455	  1.25%
 36	  45212	  1.44%
 37	  22830	  0.72%
 38	  25234	  0.80%
 39	  23047	  0.73%
 40	  32995	  1.05%
 41	  40194	  1.28%
 42	  46503	  1.48%
 43	  33981	  1.08%
 44	  25589	  0.81%
 45	  35357	  1.12%
 46	  26246	  0.83%
 47	  18744	  0.60%
 48	   1792	  0.06%
 49	   3321	  0.11%
 50	 132949	  4.22%


criterion=sequence-density
sequence-density=2.54
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=TGTAAACATCCT


criterion=fanout-score
sequence-density=0.76
sequence-density-rank=20
fanout-score=11.95
fanout-score-rank=1
prefix-density=9.06
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 07:08:11
                             Started mapping on |	Mar 04 07:08:11
                                    Finished on |	Mar 04 07:08:40
       Mapping speed, Million of reads per hour |	401.10

                          Number of input reads |	3231079
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1741428
                        Uniquely mapped reads % |	53.90%
                          Average mapped length |	24.95
                       Number of splices: Total |	2088
            Number of splices: Annotated (sjdb) |	281
                       Number of splices: GT/AG |	1942
                       Number of splices: GC/AG |	28
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	117
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.74
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1031707
             % of reads mapped to multiple loci |	31.93%
        Number of reads mapped to too many loci |	82100
             % of reads mapped to too many loci |	2.54%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.55%
                     % of reads unmapped: other |	1.08%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	457944	457944	457944
N_multimapping	1031707	1031707	1031707
N_noFeature	89246	113913	1138550
N_ambiguous	953386	422182	16625
UnstrandedReadsAssigned:698796 PositiveStrandReadsAssigned:1205333 NegativeStrandReadsAssigned:586253
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031018 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031018-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,231,079 reads, 820,439 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,171 rounds

 109283 SRR5031018.ke.tsv
  52636 SRR5031018.se.tsv
 161919 total
==> SRR5031018.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031018.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031018 completed mapping pipeline successfully
