Starting /dee2/code/volunteer_pipeline.sh SRR5031091
    current disk space = 160490815488
    free memory = 1041524584 
SRR5031091 SRAfilesize
25eb45ef3e51bbb5e0d41358efaf584f  SRR5031091.sra
SRR5031091.sra file validated
SRR5031091 is single end
SRR5031091 is conventional basespace
SRR5031091 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8495	31.0	31.0	34.0	30.0	34.0
2	32.351	33.0	31.0	34.0	31.0	34.0
3	32.3665	33.0	31.0	34.0	31.0	34.0
4	36.11525	37.0	35.0	37.0	35.0	37.0
5	36.035	37.0	35.0	37.0	35.0	37.0
6	35.9205	37.0	35.0	37.0	35.0	37.0
7	35.686	37.0	35.0	37.0	35.0	37.0
8	35.5505	37.0	35.0	37.0	33.0	37.0
9	36.81125	39.0	37.0	39.0	33.0	39.0
10	36.2345	39.0	35.0	39.0	30.0	39.0
11	37.2295	39.0	37.0	39.0	34.0	39.0
12	37.55525	39.0	37.0	39.0	35.0	39.0
13	37.46325	39.0	37.0	39.0	35.0	39.0
14	38.35675	40.0	38.0	41.0	33.0	41.0
15	36.6395	39.0	36.0	41.0	27.0	41.0
16	36.85525	38.0	36.0	40.0	31.0	41.0
17	36.9735	39.0	36.0	40.0	32.0	41.0
18	38.2835	40.0	38.0	41.0	34.0	41.0
19	38.70225	40.0	38.0	41.0	35.0	41.0
20	38.344	40.0	38.0	41.0	34.0	41.0
21	38.4525	40.0	38.0	41.0	34.0	41.0
22	37.19325	39.0	37.0	40.0	31.0	41.0
23	37.92775	39.0	37.0	40.0	34.0	41.0
24	37.06075	39.0	37.0	40.0	30.0	41.0
25	38.03	40.0	37.0	41.0	33.0	41.0
26	38.036	40.0	38.0	41.0	33.0	41.0
27	38.29275	40.0	38.0	41.0	34.0	41.0
28	38.4575	40.0	38.0	41.0	34.0	41.0
29	38.078	40.0	38.0	41.0	33.0	41.0
30	38.6195	40.0	38.0	41.0	34.0	41.0
31	38.51975	40.0	38.0	41.0	34.0	41.0
32	38.604	40.0	38.0	41.0	35.0	41.0
33	38.398	40.0	38.0	41.0	34.0	41.0
34	37.81175	40.0	38.0	41.0	32.0	41.0
35	36.93	40.0	35.0	41.0	30.0	41.0
36	32.73225	35.0	30.0	39.0	23.0	41.0
37	34.72725	37.0	33.0	39.0	29.0	40.0
38	36.31825	38.0	35.0	39.0	31.0	40.0
39	36.739	38.0	36.0	40.0	32.0	40.0
40	37.02425	39.0	37.0	40.0	32.0	41.0
41	37.45175	40.0	37.0	40.0	32.0	41.0
42	37.32325	40.0	37.0	41.0	32.0	41.0
43	37.36175	40.0	37.0	41.0	32.0	41.0
44	36.62	39.0	36.0	40.0	30.0	41.0
45	36.7755	39.0	36.0	40.0	30.0	41.0
46	36.8385	39.0	36.0	40.0	31.0	41.0
47	35.76625	38.0	34.0	40.0	28.0	41.0
48	36.906	39.0	36.0	40.0	31.0	41.0
49	36.94525	40.0	37.0	41.0	32.0	41.0
50	35.05625	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	1.0
17	2.0
18	3.0
19	2.0
20	10.0
21	1.0
22	9.0
23	6.0
24	8.0
25	8.0
26	18.0
27	21.0
28	26.0
29	40.0
30	48.0
31	81.0
32	89.0
33	133.0
34	184.0
35	282.0
36	477.0
37	762.0
38	1127.0
39	660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.55	25.775	54.75	11.924999999999999
2	26.83354192740926	26.933667083854818	18.072590738423028	28.16020025031289
3	23.400000000000002	24.5	23.200000000000003	28.9
4	33.6	29.725	12.75	23.925
5	23.075000000000003	38.175	28.775000000000002	9.975000000000001
6	33.425	15.275	37.4	13.900000000000002
7	13.750000000000002	23.75	25.15	37.35
8	29.099999999999998	28.775000000000002	13.575000000000001	28.549999999999997
9	17.9	35.9	30.8	15.4
10	23.35	22.0	25.224999999999998	29.425
11	33.725	8.325000000000001	18.825	39.125
12	16.900000000000002	16.7	31.35	35.05
13	33.300000000000004	16.025	34.825	15.85
14	35.75	26.700000000000003	22.575	14.975
15	38.800000000000004	14.6	29.7	16.900000000000002
16	17.375	16.025	27.05	39.550000000000004
17	26.85	19.75	39.7	13.700000000000001
18	29.625	17.625	27.250000000000004	25.5
19	34.25	17.025000000000002	32.0	16.725
20	45.574999999999996	15.0	27.150000000000002	12.275
21	20.025000000000002	21.175	34.475	24.325
22	29.625	8.95	49.625	11.799999999999999
23	27.250000000000004	11.35	47.0	14.399999999999999
24	51.949999999999996	11.3	27.250000000000004	9.5
25	51.7	24.775	14.45	9.075
26	30.325000000000003	47.949999999999996	14.249999999999998	7.475
27	16.225	47.75	25.75	10.274999999999999
28	11.625	23.724999999999998	51.7	12.950000000000001
29	9.475	10.325	58.074999999999996	22.125
30	11.0	5.425	38.224999999999994	45.35
31	14.05	4.05	45.475	36.425000000000004
32	28.775000000000002	5.6000000000000005	19.900000000000002	45.725
33	55.925000000000004	8.200000000000001	12.275	23.599999999999998
34	64.8	8.450000000000001	14.099999999999998	12.65
35	66.975	6.575	19.25	7.199999999999999
36	46.325	6.625	34.849999999999994	12.2
37	45.025	9.725	19.6	25.650000000000002
38	22.425	16.0	10.7	50.875
39	13.025	27.075	7.425	52.47500000000001
40	14.249999999999998	51.925	8.25	25.575
41	25.424999999999997	50.525	7.1499999999999995	16.900000000000002
42	50.275000000000006	31.45	7.675	10.6
43	51.175000000000004	32.65	6.125	10.05
44	28.725	50.175000000000004	6.0249999999999995	15.075
45	14.975	51.225	13.125	20.674999999999997
46	9.625	25.6	19.125	45.65
47	6.3	15.950000000000001	37.925	39.825
48	7.5249999999999995	13.25	19.85	59.375
49	11.725	22.95	13.125	52.2
50	19.125	36.975	14.325	29.575000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	0.5
30	0.0
31	1.0
32	2.0
33	5.5
34	9.0
35	8.0
36	7.0
37	10.5
38	14.0
39	19.5
40	25.0
41	24.5
42	24.0
43	79.0
44	134.0
45	177.5
46	221.0
47	343.5
48	466.0
49	580.5
50	695.0
51	812.5
52	930.0
53	720.0
54	510.0
55	455.0
56	400.0
57	341.5
58	283.0
59	176.0
60	69.0
61	52.5
62	36.0
63	41.0
64	46.0
65	35.0
66	24.0
67	16.5
68	9.0
69	17.5
70	26.0
71	40.5
72	55.0
73	31.5
74	8.0
75	4.0
76	0.0
77	0.0
78	0.0
79	1.5
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.90055248618785	31.724999999999998
2	9.392265193370166	7.6499999999999995
3	3.2535297728667896	3.975
4	2.394106813996317	3.9
5	1.043585021485574	2.125
6	0.9208103130755065	2.25
7	0.6752608962553714	1.925
8	0.5524861878453038	1.7999999999999998
9	0.3683241252302026	1.35
>10	2.9465930018416207	22.400000000000002
>50	0.3683241252302026	11.4
>100	0.1841620626151013	9.5
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	149	3.7249999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	126	3.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	105	2.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	100	2.5	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	85	2.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 35bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	63	1.575	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 33bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC	26	0.65	RNA PCR Primer, Index 15 (100% over 50bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	24	0.6	No Hit
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	24	0.6	RNA PCR Primer, Index 1 (96% over 30bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	22	0.5499999999999999	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG	22	0.5499999999999999	RNA PCR Primer, Index 15 (100% over 36bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC	15	0.375	RNA PCR Primer, Index 15 (100% over 44bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	15	0.375	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGACCCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	14	0.35000000000000003	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	13	0.325	RNA PCR Primer, Index 1 (100% over 35bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACCCTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CCTGTCTGAGCGTCGCTTGGACTTCTCGGGTGCCAAGGAACTCCAGTCAC	8	0.2	RNA PCR Primer, Index 1 (96% over 33bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (96% over 30bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTACCTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGACTTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 26bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	6	0.15	RNA PCR Primer, Index 1 (100% over 34bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGACTTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (96% over 26bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
TGAGGTAGTCGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA	5	0.125	RNA PCR Primer, Index 15 (100% over 39bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTAT	5	0.125	RNA PCR Primer, Index 15 (100% over 48bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGGCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT	5	0.125	RNA PCR Primer, Index 15 (100% over 37bp)
CTGCGTTCGCGCTCTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.925	0.0	0.0	0.0
2	0.0	1.05	0.0	0.0	0.0
3	0.0	1.35	0.0	0.0	0.0
4	0.0	1.375	0.0	0.0	0.0
5	0.0	1.45	0.0	0.0	0.0
6	0.0	1.7	0.0	0.0	0.0
7	0.0	2.425	0.0	0.0	0.0
8	0.0	2.6	0.0	0.0	0.0
9	0.0	2.725	0.0	0.0	0.0
10	0.0	2.925	0.0	0.0	0.0
11	0.0	3.175	0.0	0.0	0.0
12	0.0	3.55	0.0	0.0	0.0
13	0.0	3.875	0.0	0.0	0.0
14	0.0	4.35	0.0	0.0	0.0
15	0.0	5.25	0.0	0.0	0.0
16	0.0	7.825	0.0	0.0	0.0
17	0.0	8.725	0.0	0.0	0.0
18	0.0	10.175	0.0	0.0	0.0
19	0.0	10.5	0.0	0.0	0.0
20	0.0	11.675	0.0	0.0	0.0
21	0.0	16.55	0.0	0.0	0.0
22	0.0	29.95	0.0	0.0	0.0
23	0.0	60.9	0.0	0.0	0.0
24	0.0	74.65	0.0	0.0	0.0
25	0.0	79.95	0.0	0.0	0.0
26	0.0	81.3	0.0	0.0	0.0
27	0.0	81.9	0.0	0.0	0.0
28	0.0	82.525	0.0	0.0	0.0
29	0.0	83.125	0.0	0.0	0.0
30	0.0	83.575	0.0	0.0	0.0
31	0.0	84.1	0.0	0.0	0.0
32	0.0	84.65	0.0	0.0	0.0
33	0.0	85.3	0.0	0.0	0.0
34	0.0	87.075	0.0	0.0	0.0
35	0.0	87.575	0.0	0.0	0.0
36	0.0	88.1	0.0	0.0	0.0
37	0.0	88.675	0.0	0.0	0.0
38	0.0	88.95	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
AACGCTG	40	5.315087E-9	44.000004	8
TGTTGGA	40	5.315087E-9	44.000004	20
ACGCTGT	40	5.315087E-9	44.000004	9
AACCGGT	20	6.952051E-4	44.000004	9
CTGAGCT	40	5.315087E-9	44.000004	11
TTCAACG	40	5.315087E-9	44.000004	5
GGTCTCT	20	6.952051E-4	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
TCGGATC	40	5.315087E-9	44.000004	1
CATTCAA	40	5.315087E-9	44.000004	3
TCAACGC	40	5.315087E-9	44.000004	6
CGGATCC	40	5.315087E-9	44.000004	2
GTGAGTT	20	6.952051E-4	44.000004	18
ACATTCA	40	5.315087E-9	44.000004	2
GAGTTGG	20	6.952051E-4	44.000004	20
GCTGTCG	40	5.315087E-9	44.000004	11
GAACCGG	20	6.952051E-4	44.000004	8
GTCTCTT	20	6.952051E-4	44.000004	14
>>END_MODULE
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
Read 27857 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
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Read 27855 spots for SRR5031091.sra
Written 27855 spots for SRR5031091.sra
SRR ids: ['SRR5031091.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_sbg9x7u2
SRR5031091.sra spots: 1337042
blocks: [[1, 27855], [27856, 55710], [55711, 83565], [83566, 111420], [111421, 139275], [139276, 167130], [167131, 194985], [194986, 222840], [222841, 250695], [250696, 278550], [278551, 306405], [306406, 334260], [334261, 362115], [362116, 389970], [389971, 417825], [417826, 445680], [445681, 473535], [473536, 501390], [501391, 529245], [529246, 557100], [557101, 584955], [584956, 612810], [612811, 640665], [640666, 668520], [668521, 696375], [696376, 724230], [724231, 752085], [752086, 779940], [779941, 807795], [807796, 835650], [835651, 863505], [863506, 891360], [891361, 919215], [919216, 947070], [947071, 974925], [974926, 1002780], [1002781, 1030635], [1030636, 1058490], [1058491, 1086345], [1086346, 1114200], [1114201, 1142055], [1142056, 1169910], [1169911, 1197765], [1197766, 1225620], [1225621, 1253475], [1253476, 1281330], [1281331, 1309185], [1309186, 1337042]]
SRR5031091 file size 230676
SRR5031091 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR5031091 SRR5031091_1.fastq
Input file:	SRR5031091_1.fastq
trimmed:	SRR5031091-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Wed Mar  4 07:21:59 2020 >> started

Wed Mar  4 07:22:00 2020 >> done (0.862s)
1337042 reads processed; of these:
     88 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
1336948 (99.99%) reads available; of these:
 108361 ( 8.11%) trimmed reads available after processing
1228587 (91.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     32	  0.00%
 19	     55	  0.00%
 20	     71	  0.01%
 21	    111	  0.01%
 22	    187	  0.01%
 23	    231	  0.02%
 24	    244	  0.02%
 25	    342	  0.03%
 26	    233	  0.02%
 27	    302	  0.02%
 28	    372	  0.03%
 29	    647	  0.05%
 30	    398	  0.03%
 31	    453	  0.03%
 32	    593	  0.04%
 33	   1035	  0.08%
 34	   1496	  0.11%
 35	   3326	  0.25%
 36	   2075	  0.16%
 37	   1869	  0.14%
 38	   1780	  0.13%
 39	   2105	  0.16%
 40	   2665	  0.20%
 41	   2706	  0.20%
 42	   3313	  0.25%
 43	   5117	  0.38%
 44	   5431	  0.41%
 45	   7068	  0.53%
 46	   8546	  0.64%
 47	   8468	  0.63%
 48	  22341	  1.67%
 49	  24749	  1.85%
 50	1228587	 91.89%
1336948 reads passed initial QC


criterion=sequence-density
sequence-density=90.97
sequence-density-rank=1
fanout-score=18.86
fanout-score-rank=2
prefix-density=91.12
prefix-fanout=18.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC


criterion=fanout-score
sequence-density=3.98
sequence-density-rank=2
fanout-score=63.40
fanout-score-rank=1
prefix-density=91.70
prefix-fanout=2.8
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC -o SRR5031091 -
Input file:	STDIN
trimmed:	SRR5031091-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Wed Mar  4 07:22:10 2020 >> started

Wed Mar  4 07:22:12 2020 >> done (1.956s)
1307565 reads processed; of these:
 136203 (10.42%) short reads filtered out after trimming by size control
  13990 ( 1.07%) empty reads filtered out after trimming by size control
1157372 (88.51%) reads available; of these:
1120601 (96.82%) trimmed reads available after processing
  36771 ( 3.18%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   7965	  0.69%
 19	  19101	  1.65%
 20	  78075	  6.75%
 21	 169731	 14.67%
 22	 405455	 35.03%
 23	 190132	 16.43%
 24	  83549	  7.22%
 25	  19633	  1.70%
 26	   9797	  0.85%
 27	   7895	  0.68%
 28	   7777	  0.67%
 29	   5710	  0.49%
 30	   8179	  0.71%
 31	  10396	  0.90%
 32	  14214	  1.23%
 33	  23671	  2.05%
 34	   9125	  0.79%
 35	   6303	  0.54%
 36	   6756	  0.58%
 37	   3669	  0.32%
 38	   3459	  0.30%
 39	   3117	  0.27%
 40	   3775	  0.33%
 41	   4800	  0.41%
 42	   4931	  0.43%
 43	   3859	  0.33%
 44	   2671	  0.23%
 45	   5526	  0.48%
 46	   4266	  0.37%
 47	   2775	  0.24%
 48	    430	  0.04%
 49	    446	  0.04%
 50	  30184	  2.61%


criterion=sequence-density
sequence-density=9.82
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.34
sequence-density-rank=7
fanout-score=17.94
fanout-score-rank=1
prefix-density=2.31
prefix-fanout=17.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC
                                 Started job on |	Mar 04 07:23:42
                             Started mapping on |	Mar 04 07:23:42
                                    Finished on |	Mar 04 07:24:12
       Mapping speed, Million of reads per hour |	142.41

                          Number of input reads |	1186755
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	597292
                        Uniquely mapped reads % |	50.33%
                          Average mapped length |	23.19
                       Number of splices: Total |	1765
            Number of splices: Annotated (sjdb) |	285
                       Number of splices: GT/AG |	1720
                       Number of splices: GC/AG |	30
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	15
                      Mismatch rate per base, % |	0.34%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.90
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	474524
             % of reads mapped to multiple loci |	39.99%
        Number of reads mapped to too many loci |	21593
             % of reads mapped to too many loci |	1.82%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.32%
                     % of reads unmapped: other |	0.55%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	114939	114939	114939
N_multimapping	474524	474524	474524
N_noFeature	31429	35826	424015
N_ambiguous	324467	170004	3084
UnstrandedReadsAssigned:241396 PositiveStrandReadsAssigned:391462 NegativeStrandReadsAssigned:170193
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031091 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031091-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,186,755 reads, 274,325 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,000 rounds

 109283 SRR5031091.ke.tsv
  52636 SRR5031091.se.tsv
 161919 total
==> SRR5031091.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031091.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031091 completed mapping pipeline successfully
