Starting /dee2/code/volunteer_pipeline.sh SRR5030930
    current disk space = 160611975168
    free memory = 73918884 
SRR5030930 SRAfilesize
4858a016708315407aa47c7b2aab1a3c  SRR5030930.sra
SRR5030930.sra file validated
SRR5030930 is single end
SRR5030930 is conventional basespace
SRR5030930 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030930_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.489	34.0	31.0	34.0	31.0	34.0
2	32.7295	34.0	31.0	34.0	31.0	34.0
3	32.854	34.0	31.0	34.0	31.0	34.0
4	36.308	37.0	37.0	37.0	35.0	37.0
5	36.34325	37.0	37.0	37.0	35.0	37.0
6	36.10225	37.0	36.0	37.0	35.0	37.0
7	36.15475	37.0	36.0	37.0	35.0	37.0
8	36.18825	37.0	36.0	37.0	35.0	37.0
9	37.996	39.0	38.0	39.0	35.0	39.0
10	37.955	39.0	38.0	39.0	35.0	39.0
11	37.91175	39.0	38.0	39.0	35.0	39.0
12	37.937	39.0	38.0	39.0	35.0	39.0
13	37.865	39.0	38.0	39.0	35.0	39.0
14	39.3795	41.0	39.0	41.0	36.0	41.0
15	39.3845	41.0	39.0	41.0	36.0	41.0
16	39.362	41.0	39.0	41.0	36.0	41.0
17	39.54325	41.0	39.0	41.0	36.0	41.0
18	39.37975	41.0	39.0	41.0	36.0	41.0
19	39.0935	41.0	39.0	41.0	36.0	41.0
20	39.202	40.0	39.0	41.0	36.0	41.0
21	39.2485	41.0	39.0	41.0	36.0	41.0
22	39.29875	41.0	39.0	41.0	36.0	41.0
23	39.01075	40.0	39.0	41.0	36.0	41.0
24	38.81175	40.0	39.0	41.0	35.0	41.0
25	39.14	41.0	39.0	41.0	36.0	41.0
26	39.08075	41.0	39.0	41.0	36.0	41.0
27	39.1555	41.0	39.0	41.0	36.0	41.0
28	38.929	41.0	39.0	41.0	35.0	41.0
29	38.3425	40.0	38.0	41.0	33.0	41.0
30	38.712	40.0	38.0	41.0	34.0	41.0
31	38.9705	40.0	39.0	41.0	35.0	41.0
32	39.06775	40.0	39.0	41.0	35.0	41.0
33	38.2725	40.0	38.0	41.0	34.0	41.0
34	38.23675	40.0	38.0	41.0	34.0	41.0
35	37.74325	39.0	38.0	40.0	33.0	41.0
36	34.15925	38.0	31.0	40.0	23.0	41.0
37	35.9405	38.0	35.0	40.0	30.0	41.0
38	37.21	39.0	37.0	40.0	32.0	41.0
39	37.29425	39.0	37.0	40.0	31.0	41.0
40	37.32825	40.0	37.0	41.0	32.0	41.0
41	37.455	40.0	37.0	41.0	32.0	41.0
42	37.71925	40.0	38.0	41.0	33.0	41.0
43	37.14925	40.0	37.0	41.0	31.0	41.0
44	37.223	40.0	37.0	41.0	31.0	41.0
45	36.931	39.0	36.0	41.0	31.0	41.0
46	35.074	38.0	34.0	40.0	26.0	41.0
47	35.85775	39.0	35.0	40.0	28.0	41.0
48	36.2195	39.0	36.0	41.0	30.0	41.0
49	35.193	39.0	35.0	40.0	28.0	41.0
50	30.0515	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	4.0
20	4.0
21	7.0
22	7.0
23	4.0
24	9.0
25	9.0
26	18.0
27	16.0
28	34.0
29	52.0
30	54.0
31	66.0
32	84.0
33	100.0
34	136.0
35	192.0
36	296.0
37	514.0
38	992.0
39	1401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.389083625438158	20.180270405608415	57.1607411116675	13.26990485728593
2	24.725	25.474999999999998	22.475	27.325
3	19.15	19.0	20.625	41.225
4	27.450000000000003	35.3	12.975	24.275
5	20.875	40.8	26.325	12.0
6	34.475	16.225	37.25	12.049999999999999
7	15.475	23.849999999999998	24.375	36.3
8	23.799999999999997	40.025	14.124999999999998	22.05
9	20.175	36.625	30.25	12.950000000000001
10	21.5	20.349999999999998	27.0	31.15
11	30.2	13.875000000000002	14.475	41.449999999999996
12	15.6	17.125	26.150000000000002	41.125
13	20.4	22.125	36.375	21.099999999999998
14	36.1	24.15	26.0	13.750000000000002
15	37.95	22.7	27.625	11.725
16	13.5	28.175	20.724999999999998	37.6
17	22.675	23.75	40.1	13.475000000000001
18	27.650000000000002	18.9	29.425	24.025
19	39.35	13.0	29.575000000000003	18.075
20	46.949999999999996	17.7	23.7	11.65
21	22.900000000000002	24.224999999999998	24.05	28.825
22	32.225	12.275	48.025	7.475
23	25.374999999999996	13.200000000000001	48.25	13.175
24	53.2	14.099999999999998	25.674999999999997	7.025
25	53.300000000000004	22.975	14.299999999999999	9.425
26	26.950000000000003	48.699999999999996	13.450000000000001	10.9
27	14.249999999999998	51.65	23.875	10.225
28	9.825000000000001	24.775	51.225	14.174999999999999
29	9.9	11.575000000000001	59.8	18.725
30	8.95	6.8500000000000005	35.9	48.3
31	13.950000000000001	5.475	47.8	32.775
32	26.35	5.8999999999999995	22.125	45.625
33	56.574999999999996	7.1499999999999995	12.875	23.400000000000002
34	65.225	7.000000000000001	14.899999999999999	12.875
35	66.2	7.025	17.150000000000002	9.625
36	42.525	7.95	37.625	11.899999999999999
37	46.9	10.424999999999999	20.424999999999997	22.25
38	22.55	14.374999999999998	11.799999999999999	51.275000000000006
39	13.475000000000001	24.025	8.674999999999999	53.825
40	13.850000000000001	51.2	9.8	25.15
41	23.175	53.925	8.5	14.399999999999999
42	52.5	29.95	9.375	8.175
43	54.85	28.225	8.200000000000001	8.725
44	28.849999999999998	51.075	7.3	12.775
45	16.2	53.5	11.475	18.825
46	10.375	26.75	19.8	43.075
47	8.9	14.000000000000002	40.9	36.199999999999996
48	9.75	12.174999999999999	20.3	57.775
49	12.675	19.175	13.075000000000001	55.074999999999996
50	18.775	39.925	11.85	29.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	0.0
29	0.5
30	1.0
31	8.0
32	15.0
33	17.0
34	19.0
35	27.0
36	35.0
37	41.5
38	48.0
39	54.5
40	61.0
41	54.0
42	47.0
43	85.0
44	123.0
45	181.0
46	239.0
47	411.5
48	584.0
49	611.5
50	639.0
51	772.0
52	905.0
53	634.5
54	364.0
55	375.0
56	386.0
57	327.5
58	269.0
59	165.5
60	62.0
61	50.5
62	39.0
63	61.5
64	84.0
65	51.0
66	18.0
67	11.5
68	5.0
69	9.5
70	14.0
71	21.0
72	28.0
73	16.5
74	5.0
75	3.5
76	2.0
77	2.0
78	2.0
79	1.0
80	0.0
81	1.5
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.324999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34246575342466	28.875
2	9.980430528375733	7.6499999999999995
3	3.7181996086105675	4.275
4	2.152641878669276	3.3000000000000003
5	1.2393998695368558	2.375
6	1.1741682974559686	2.7
7	1.0437051532941943	2.8000000000000003
8	0.91324200913242	2.8000000000000003
9	0.5218525766470972	1.7999999999999998
>10	3.457273320287019	24.575
>50	0.19569471624266144	4.15
>100	0.2609262883235486	14.7
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	189	4.725	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	141	3.5249999999999995	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	131	3.2750000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	127	3.175	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	59	1.4749999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	55	1.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	52	1.3	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	43	1.075	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	19	0.475	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTC	16	0.4	RNA PCR Primer, Index 43 (100% over 44bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	15	0.375	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	13	0.325	RNA PCR Primer, Index 7 (97% over 35bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	12	0.3	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	12	0.3	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	11	0.27499999999999997	No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGC	11	0.27499999999999997	RNA PCR Primer, Index 43 (100% over 50bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	9	0.22499999999999998	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	9	0.22499999999999998	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	9	0.22499999999999998	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGAAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	8	0.2	No Hit
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	7	0.17500000000000002	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	6	0.15	RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	6	0.15	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	6	0.15	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTAT	5	0.125	RNA PCR Primer, Index 43 (100% over 48bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCCAGGAACTCCAGTCACTA	5	0.125	RNA PCR Primer, Index 10 (97% over 35bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.275	0.0	0.0	0.0
2	0.0	0.275	0.0	0.0	0.0
3	0.0	0.4	0.0	0.0	0.0
4	0.0	0.4	0.0	0.0	0.0
5	0.0	0.425	0.0	0.0	0.0
6	0.0	0.5	0.0	0.0	0.0
7	0.0	0.95	0.0	0.0	0.0
8	0.0	1.05	0.0	0.0	0.0
9	0.0	1.15	0.0	0.0	0.0
10	0.0	1.325	0.0	0.0	0.0
11	0.0	1.45	0.0	0.0	0.0
12	0.0	1.65	0.0	0.0	0.0
13	0.0	2.025	0.0	0.0	0.0
14	0.0	2.3	0.0	0.0	0.0
15	0.0	2.6	0.0	0.0	0.0
16	0.0	3.275	0.0	0.0	0.0
17	0.0	3.9	0.0	0.0	0.0
18	0.0	4.675	0.0	0.0	0.0
19	0.0	5.275	0.0	0.0	0.0
20	0.0	6.425	0.0	0.0	0.0
21	0.0	11.775	0.0	0.0	0.0
22	0.0	22.7	0.0	0.0	0.0
23	0.0	55.75	0.0	0.0	0.0
24	0.0	69.4	0.0	0.0	0.0
25	0.0	74.725	0.0	0.0	0.0
26	0.0	75.625	0.0	0.0	0.0
27	0.0	75.9	0.0	0.0	0.0
28	0.0	76.2	0.0	0.0	0.0
29	0.0	76.5	0.0	0.0	0.0
30	0.0	76.8	0.0	0.0	0.0
31	0.0	77.525	0.0	0.0	0.0
32	0.0	78.475	0.0	0.0	0.0
33	0.0	79.575	0.0	0.0	0.0
34	0.0	81.575	0.0	0.0	0.0
35	0.0	82.525	0.0	0.0	0.0
36	0.0	83.175	0.0	0.0	0.0
37	0.0	83.725	0.0	0.0	0.0
38	0.0	84.175	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
GGTTGTA	20	6.952051E-4	44.000004	11
GGTGAGT	20	6.952051E-4	44.000004	17
AAGTAAT	45	2.728484E-10	44.000004	4
TCAAGTA	45	2.728484E-10	44.000004	2
ACGCTGT	20	6.952051E-4	44.000004	9
AATCCAG	45	2.728484E-10	44.000004	8
TAAACAT	45	2.728484E-10	44.000004	3
ATCCAGG	45	2.728484E-10	44.000004	9
TTGTATA	20	6.952051E-4	44.000004	13
AAACATC	45	2.728484E-10	44.000004	4
AGTAGGT	20	6.952051E-4	44.000004	7
TTCAAGT	45	2.728484E-10	44.000004	1
TTCAACG	20	6.952051E-4	44.000004	5
AGGATAG	45	2.728484E-10	44.000004	13
TCGGTGA	20	6.952051E-4	44.000004	15
ATAGGCT	45	2.728484E-10	44.000004	16
CATTCAA	20	6.952051E-4	44.000004	3
TCAACGC	20	6.952051E-4	44.000004	6
TCCAGGA	45	2.728484E-10	44.000004	10
>>END_MODULE
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304146 spots for SRR5030930.sra
Written 304146 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
Read 304136 spots for SRR5030930.sra
Written 304136 spots for SRR5030930.sra
SRR ids: ['SRR5030930.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_m37dw6rc
SRR5030930.sra spots: 3649642
blocks: [[1, 304136], [304137, 608272], [608273, 912408], [912409, 1216544], [1216545, 1520680], [1520681, 1824816], [1824817, 2128952], [2128953, 2433088], [2433089, 2737224], [2737225, 3041360], [3041361, 3345496], [3345497, 3649642]]
SRR5030930 file size 631490
SRR5030930 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030930 SRR5030930_1.fastq
Input file:	SRR5030930_1.fastq
trimmed:	SRR5030930-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:47:41 2020 >> started

Wed Mar  4 06:47:44 2020 >> done (3.751s)
3649642 reads processed; of these:
    137 ( 0.00%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
3649502 (100.00%) reads available; of these:
 201541 ( 5.52%) trimmed reads available after processing
3447961 (94.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     37	  0.00%
 19	     50	  0.00%
 20	    141	  0.00%
 21	    177	  0.00%
 22	    331	  0.01%
 23	    354	  0.01%
 24	    396	  0.01%
 25	    514	  0.01%
 26	    395	  0.01%
 27	    523	  0.01%
 28	    709	  0.02%
 29	   1301	  0.04%
 30	    796	  0.02%
 31	    978	  0.03%
 32	   1047	  0.03%
 33	   1135	  0.03%
 34	   3482	  0.10%
 35	   2641	  0.07%
 36	   2082	  0.06%
 37	   2152	  0.06%
 38	   2415	  0.07%
 39	   2688	  0.07%
 40	   3079	  0.08%
 41	   3555	  0.10%
 42	   5239	  0.14%
 43	   6505	  0.18%
 44	  12094	  0.33%
 45	  15160	  0.42%
 46	  12793	  0.35%
 47	  18707	  0.51%
 48	  40580	  1.11%
 49	  59485	  1.63%
 50	3447961	 94.48%
3649502 reads passed initial QC


criterion=sequence-density
sequence-density=84.97
sequence-density-rank=1
fanout-score=18.33
fanout-score-rank=1
prefix-density=86.54
prefix-fanout=18.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC


criterion=fanout-score
sequence-density=84.97
sequence-density-rank=1
fanout-score=18.33
fanout-score-rank=1
prefix-density=86.54
prefix-fanout=18.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC -o SRR5030930 -
Input file:	STDIN
trimmed:	SRR5030930-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACAGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:48:09 2020 >> started

Wed Mar  4 06:48:18 2020 >> done (9.504s)
3563631 reads processed; of these:
 160878 ( 4.51%) short reads filtered out after trimming by size control
  12333 ( 0.35%) empty reads filtered out after trimming by size control
3390420 (95.14%) reads available; of these:
3101277 (91.47%) trimmed reads available after processing
 289143 ( 8.53%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  19295	  0.57%
 19	  40210	  1.19%
 20	 173897	  5.13%
 21	 390417	 11.52%
 22	1179969	 34.80%
 23	 552599	 16.30%
 24	 200302	  5.91%
 25	  33415	  0.99%
 26	  16856	  0.50%
 27	  16535	  0.49%
 28	  18517	  0.55%
 29	  13950	  0.41%
 30	  25050	  0.74%
 31	  33770	  1.00%
 32	  36191	  1.07%
 33	  83097	  2.45%
 34	  31045	  0.92%
 35	  19375	  0.57%
 36	  20684	  0.61%
 37	  13677	  0.40%
 38	  14480	  0.43%
 39	  14286	  0.42%
 40	  19161	  0.57%
 41	  26915	  0.79%
 42	  28905	  0.85%
 43	  21939	  0.65%
 44	  19522	  0.58%
 45	  22710	  0.67%
 46	  17482	  0.52%
 47	  15308	  0.45%
 48	   2912	  0.09%
 49	   9623	  0.28%
 50	 258326	  7.62%


criterion=sequence-density
sequence-density=10.06
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=31
fanout-score=16.02
fanout-score-rank=1
prefix-density=6.70
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:48:49
                             Started mapping on |	Mar 04 06:48:50
                                    Finished on |	Mar 04 06:49:14
       Mapping speed, Million of reads per hour |	521.44

                          Number of input reads |	3476291
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1756690
                        Uniquely mapped reads % |	50.53%
                          Average mapped length |	26.19
                       Number of splices: Total |	1549
            Number of splices: Annotated (sjdb) |	195
                       Number of splices: GT/AG |	1414
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	118
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.64
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1354627
             % of reads mapped to multiple loci |	38.97%
        Number of reads mapped to too many loci |	82364
             % of reads mapped to too many loci |	2.37%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.50%
                     % of reads unmapped: other |	0.63%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	364974	364974	364974
N_multimapping	1354627	1354627	1354627
N_noFeature	67359	82061	1347111
N_ambiguous	964950	614722	13534
UnstrandedReadsAssigned:724381 PositiveStrandReadsAssigned:1059907 NegativeStrandReadsAssigned:396045
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030930 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030930-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,476,291 reads, 735,164 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 657 rounds

 109283 SRR5030930.ke.tsv
  52636 SRR5030930.se.tsv
 161919 total
==> SRR5030930.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030930.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030930 completed mapping pipeline successfully
