Starting /dee2/code/volunteer_pipeline.sh SRR5030912
    current disk space = 160639074304
    free memory = 1046818220 
SRR5030912 SRAfilesize
bf6fd67747e07df60eef139b40146eeb  SRR5030912.sra
SRR5030912.sra file validated
SRR5030912 is single end
SRR5030912 is conventional basespace
SRR5030912 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.451	33.0	31.0	34.0	31.0	34.0
2	32.755	34.0	31.0	34.0	31.0	34.0
3	32.694	34.0	31.0	34.0	31.0	34.0
4	36.25225	37.0	35.0	37.0	35.0	37.0
5	36.29525	37.0	37.0	37.0	35.0	37.0
6	36.08325	37.0	35.0	37.0	35.0	37.0
7	36.0955	37.0	35.0	37.0	35.0	37.0
8	36.129	37.0	36.0	37.0	35.0	37.0
9	37.9265	39.0	38.0	39.0	35.0	39.0
10	37.80375	39.0	38.0	39.0	35.0	39.0
11	37.93175	39.0	38.0	39.0	35.0	39.0
12	37.68	39.0	38.0	39.0	35.0	39.0
13	37.75375	39.0	38.0	39.0	35.0	39.0
14	39.23375	41.0	39.0	41.0	36.0	41.0
15	39.276	41.0	39.0	41.0	36.0	41.0
16	39.15425	40.0	39.0	41.0	36.0	41.0
17	39.17975	41.0	39.0	41.0	36.0	41.0
18	39.285	41.0	39.0	41.0	36.0	41.0
19	38.96175	40.0	38.0	41.0	35.0	41.0
20	39.0145	40.0	39.0	41.0	36.0	41.0
21	39.065	40.0	39.0	41.0	36.0	41.0
22	39.41325	41.0	39.0	41.0	36.0	41.0
23	39.27925	41.0	39.0	41.0	36.0	41.0
24	38.568	40.0	38.0	41.0	34.0	41.0
25	38.68075	40.0	38.0	41.0	34.0	41.0
26	38.9075	40.0	38.0	41.0	35.0	41.0
27	39.00225	40.0	39.0	41.0	35.0	41.0
28	38.93375	40.0	38.0	41.0	35.0	41.0
29	38.41575	40.0	38.0	41.0	34.0	41.0
30	38.74275	40.0	38.0	41.0	34.0	41.0
31	39.0235	41.0	39.0	41.0	35.0	41.0
32	38.96675	41.0	38.0	41.0	35.0	41.0
33	37.9885	40.0	38.0	41.0	33.0	41.0
34	37.86575	40.0	38.0	41.0	32.0	41.0
35	37.6095	40.0	37.0	41.0	32.0	41.0
36	32.21225	37.0	26.0	40.0	15.0	41.0
37	34.88075	37.0	32.0	39.0	26.0	41.0
38	36.74825	38.0	36.0	40.0	31.0	41.0
39	37.096	39.0	37.0	40.0	31.0	41.0
40	37.13275	39.0	37.0	40.0	32.0	41.0
41	36.93375	39.0	36.0	40.0	31.0	41.0
42	36.987	39.0	37.0	40.0	31.0	41.0
43	36.96225	39.0	37.0	41.0	31.0	41.0
44	37.10175	40.0	37.0	41.0	32.0	41.0
45	36.51875	39.0	36.0	41.0	30.0	41.0
46	34.3045	38.0	33.0	40.0	23.0	41.0
47	35.57625	39.0	35.0	40.0	28.0	41.0
48	35.732	39.0	35.0	40.0	29.0	41.0
49	34.70225	38.0	34.0	40.0	26.0	41.0
50	30.1525	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	0.0
19	3.0
20	6.0
21	7.0
22	7.0
23	10.0
24	13.0
25	20.0
26	19.0
27	25.0
28	27.0
29	52.0
30	53.0
31	68.0
32	98.0
33	121.0
34	159.0
35	212.0
36	303.0
37	508.0
38	1056.0
39	1230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.291583166332666	21.29258517034068	56.563126252505015	13.852705410821645
2	21.825	26.674999999999997	23.875	27.625
3	19.375	17.775	19.425	43.425000000000004
4	23.375	38.625	13.375	24.625
5	18.425	42.4	26.724999999999998	12.45
6	38.175	16.875	33.775	11.175
7	15.024999999999999	21.875	27.025	36.075
8	22.675	42.625	14.299999999999999	20.4
9	19.1	39.925	28.875	12.1
10	20.674999999999997	18.55	29.825000000000003	30.95
11	27.875	12.675	15.15	44.3
12	13.925	17.325	25.874999999999996	42.875
13	20.75	25.825	33.45	19.975
14	38.224999999999994	26.224999999999998	23.45	12.1
15	38.15	22.5	28.000000000000004	11.35
16	13.200000000000001	29.45	20.1	37.25
17	23.05	21.099999999999998	42.425000000000004	13.425
18	27.900000000000002	21.05	27.825	23.225
19	39.574999999999996	14.124999999999998	29.625	16.675
20	48.8	18.7	22.125	10.375
21	20.9	23.9	24.025	31.175000000000004
22	31.225	13.15	47.099999999999994	8.525
23	25.124999999999996	13.4	48.25	13.225000000000001
24	51.300000000000004	13.725000000000001	27.925	7.049999999999999
25	54.05	22.325	14.124999999999998	9.5
26	28.775000000000002	47.949999999999996	13.225000000000001	10.05
27	14.224999999999998	52.75	22.85	10.174999999999999
28	9.675	25.85	50.74999999999999	13.725000000000001
29	9.1	12.1	60.975	17.825
30	9.2	6.05	36.9	47.85
31	13.275	5.1499999999999995	47.675	33.900000000000006
32	25.424999999999997	5.7250000000000005	23.05	45.800000000000004
33	55.900000000000006	7.35	13.100000000000001	23.65
34	65.375	6.75	13.850000000000001	14.025000000000002
35	66.9	7.049999999999999	16.650000000000002	9.4
36	45.275	7.000000000000001	34.775	12.950000000000001
37	47.225	8.075000000000001	22.05	22.650000000000002
38	24.575	14.000000000000002	12.1	49.325
39	14.95	23.075000000000003	8.0	53.974999999999994
40	14.075	50.125	10.35	25.45
41	23.724999999999998	53.1	9.225	13.950000000000001
42	51.824999999999996	31.35	9.1	7.725
43	55.300000000000004	28.9	8.1	7.7
44	30.099999999999998	50.675000000000004	6.7250000000000005	12.5
45	15.8	55.725	12.1	16.375
46	10.6	28.599999999999998	17.5	43.3
47	8.450000000000001	15.0	40.925	35.625
48	9.825000000000001	12.45	21.7	56.025000000000006
49	14.549999999999999	18.175	13.025	54.25
50	18.25	37.7	13.5	30.55
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	1.0
24	2.0
25	1.5
26	1.0
27	1.5
28	2.0
29	2.5
30	3.0
31	8.5
32	14.0
33	16.5
34	19.0
35	36.0
36	53.0
37	48.0
38	43.0
39	43.5
40	44.0
41	50.0
42	56.0
43	83.5
44	111.0
45	186.5
46	262.0
47	400.0
48	538.0
49	620.0
50	702.0
51	823.5
52	945.0
53	657.0
54	369.0
55	356.5
56	344.0
57	300.5
58	257.0
59	159.0
60	61.0
61	47.0
62	33.0
63	45.0
64	57.0
65	40.0
66	23.0
67	15.0
68	7.0
69	11.5
70	16.0
71	21.5
72	27.0
73	15.5
74	4.0
75	2.5
76	1.0
77	2.0
78	3.0
79	2.5
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.98362802881466	28.625
2	9.692206941715781	7.3999999999999995
3	4.715127701375246	5.4
4	1.5717092337917484	2.4
5	1.5062213490504257	2.875
6	1.2442698100851344	2.85
7	1.309757694826457	3.5000000000000004
8	0.8513425016371972	2.6
9	0.6548788474132285	2.25
>10	3.0124426981008514	22.2
>50	0.26195153896529144	7.75
>100	0.19646365422396855	12.15
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	227	5.675	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	141	3.5249999999999995	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	96	2.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	61	1.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	49	1.225	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	42	1.05	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	30	0.75	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	24	0.6	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	23	0.575	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	16	0.4	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	15	0.375	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	15	0.375	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	10	0.25	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTC	10	0.25	RNA PCR Primer, Index 17 (100% over 44bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	9	0.22499999999999998	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 17 (100% over 50bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	8	0.2	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	8	0.2	RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	8	0.2	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 33bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	6	0.15	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
GTACAGTACTGTGATAACTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	6	0.15	RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA	5	0.125	RNA PCR Primer, Index 3 (97% over 36bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	5	0.125	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.25	0.0	0.0	0.0
3	0.0	0.3	0.0	0.0	0.0
4	0.0	0.3	0.0	0.0	0.0
5	0.0	0.3	0.0	0.0	0.0
6	0.0	0.375	0.0	0.0	0.0
7	0.0	0.725	0.0	0.0	0.0
8	0.0	0.825	0.0	0.0	0.0
9	0.0	0.875	0.0	0.0	0.0
10	0.0	0.95	0.0	0.0	0.0
11	0.0	1.1	0.0	0.0	0.0
12	0.0	1.225	0.0	0.0	0.0
13	0.0	1.4	0.0	0.0	0.0
14	0.0	1.5	0.0	0.0	0.0
15	0.0	1.9	0.0	0.0	0.0
16	0.0	2.6	0.0	0.0	0.0
17	0.0	3.05	0.0	0.0	0.0
18	0.0	3.85	0.0	0.0	0.0
19	0.0	4.225	0.0	0.0	0.0
20	0.0	5.025	0.0	0.0	0.0
21	0.0	10.375	0.0	0.0	0.0
22	0.0	20.825	0.0	0.0	0.0
23	0.0	53.525	0.0	0.0	0.0
24	0.0	68.4	0.0	0.0	0.0
25	0.0	74.125	0.0	0.0	0.0
26	0.0	75.175	0.0	0.0	0.0
27	0.0	75.7	0.0	0.0	0.0
28	0.0	76.225	0.0	0.0	0.0
29	0.0	76.525	0.0	0.0	0.0
30	0.0	76.9	0.0	0.0	0.0
31	0.0	77.425	0.0	0.0	0.0
32	0.0	78.2	0.0	0.0	0.0
33	0.0	78.775	0.0	0.0	0.0
34	0.0	80.375	0.0	0.0	0.0
35	0.0	81.45	0.0	0.0	0.0
36	0.0	82.15	0.0	0.0	0.0
37	0.0	83.125	0.0	0.0	0.0
38	0.0	83.4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGCT	20	6.952051E-4	44.000004	11
AAATCGG	20	6.952051E-4	44.000004	13
GAAATCG	20	6.952051E-4	44.000004	12
ACCATCT	20	6.952051E-4	44.000004	5
GAGCTTG	20	6.952051E-4	44.000004	13
GGATCCG	20	6.952051E-4	44.000004	3
CCGTCTG	20	6.952051E-4	44.000004	7
TCGGATC	20	6.952051E-4	44.000004	1
CGGATCC	20	6.952051E-4	44.000004	2
GTGAGTT	20	6.952051E-4	44.000004	18
TAGCACC	20	6.952051E-4	44.000004	1
GAGTTGG	20	6.952051E-4	44.000004	20
ATCGGTT	20	6.952051E-4	44.000004	15
GATCCGT	20	6.952051E-4	44.000004	4
CACCATC	20	6.952051E-4	44.000004	4
AGCACCA	20	6.952051E-4	44.000004	2
TCCGTCT	20	6.952051E-4	44.000004	6
GCACCAT	20	6.952051E-4	44.000004	3
ATCCGTC	20	6.952051E-4	44.000004	5
AATCGGT	20	6.952051E-4	44.000004	14
>>END_MODULE
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
Read 105858 spots for SRR5030912.sra
Written 105858 spots for SRR5030912.sra
Read 105854 spots for SRR5030912.sra
Written 105854 spots for SRR5030912.sra
SRR ids: ['SRR5030912.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ec4lybfe
SRR5030912.sra spots: 5080996
blocks: [[1, 105854], [105855, 211708], [211709, 317562], [317563, 423416], [423417, 529270], [529271, 635124], [635125, 740978], [740979, 846832], [846833, 952686], [952687, 1058540], [1058541, 1164394], [1164395, 1270248], [1270249, 1376102], [1376103, 1481956], [1481957, 1587810], [1587811, 1693664], [1693665, 1799518], [1799519, 1905372], [1905373, 2011226], [2011227, 2117080], [2117081, 2222934], [2222935, 2328788], [2328789, 2434642], [2434643, 2540496], [2540497, 2646350], [2646351, 2752204], [2752205, 2858058], [2858059, 2963912], [2963913, 3069766], [3069767, 3175620], [3175621, 3281474], [3281475, 3387328], [3387329, 3493182], [3493183, 3599036], [3599037, 3704890], [3704891, 3810744], [3810745, 3916598], [3916599, 4022452], [4022453, 4128306], [4128307, 4234160], [4234161, 4340014], [4340015, 4445868], [4445869, 4551722], [4551723, 4657576], [4657577, 4763430], [4763431, 4869284], [4869285, 4975138], [4975139, 5080996]]
SRR5030912 file size 879571
SRR5030912 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR5030912 SRR5030912_1.fastq
Input file:	SRR5030912_1.fastq
trimmed:	SRR5030912-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Wed Mar  4 06:43:10 2020 >> started

Wed Mar  4 06:43:13 2020 >> done (3.117s)
5080996 reads processed; of these:
    234 ( 0.00%) short reads filtered out after trimming by size control
      9 ( 0.00%) empty reads filtered out after trimming by size control
5080753 (100.00%) reads available; of these:
 392023 ( 7.72%) trimmed reads available after processing
4688730 (92.28%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     77	  0.00%
 19	     83	  0.00%
 20	    236	  0.00%
 21	    279	  0.01%
 22	    486	  0.01%
 23	    596	  0.01%
 24	    546	  0.01%
 25	    664	  0.01%
 26	    623	  0.01%
 27	    803	  0.02%
 28	    998	  0.02%
 29	   1688	  0.03%
 30	   1253	  0.02%
 31	   1645	  0.03%
 32	   2357	  0.05%
 33	   3825	  0.08%
 34	  14547	  0.29%
 35	   7286	  0.14%
 36	   5314	  0.10%
 37	   4528	  0.09%
 38	   7470	  0.15%
 39	   6008	  0.12%
 40	  10942	  0.22%
 41	   7834	  0.15%
 42	  13912	  0.27%
 43	  10053	  0.20%
 44	  31747	  0.62%
 45	  26504	  0.52%
 46	  22714	  0.45%
 47	  27925	  0.55%
 48	  76488	  1.51%
 49	 102592	  2.02%
 50	4688730	 92.28%
5080753 reads passed initial QC


criterion=sequence-density
sequence-density=82.95
sequence-density-rank=1
fanout-score=18.07
fanout-score-rank=2
prefix-density=84.95
prefix-fanout=17.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC


criterion=fanout-score
sequence-density=2.93
sequence-density-rank=4
fanout-score=86.68
fanout-score-rank=1
prefix-density=84.94
prefix-fanout=3.0
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC -o SRR5030912 -
Input file:	STDIN
trimmed:	SRR5030912-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Wed Mar  4 06:43:38 2020 >> started

Wed Mar  4 06:43:46 2020 >> done (7.577s)
4958325 reads processed; of these:
 179481 ( 3.62%) short reads filtered out after trimming by size control
  17017 ( 0.34%) empty reads filtered out after trimming by size control
4761827 (96.04%) reads available; of these:
4312918 (90.57%) trimmed reads available after processing
 448909 ( 9.43%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  25791	  0.54%
 19	  51303	  1.08%
 20	 244424	  5.13%
 21	 550919	 11.57%
 22	1640971	 34.46%
 23	 740338	 15.55%
 24	 269788	  5.67%
 25	  45974	  0.97%
 26	  21721	  0.46%
 27	  21179	  0.44%
 28	  23206	  0.49%
 29	  17411	  0.37%
 30	  33930	  0.71%
 31	  43085	  0.90%
 32	  46492	  0.98%
 33	  84311	  1.77%
 34	  46614	  0.98%
 35	  36036	  0.76%
 36	  42954	  0.90%
 37	  21860	  0.46%
 38	  23693	  0.50%
 39	  24977	  0.52%
 40	  32848	  0.69%
 41	  45192	  0.95%
 42	  50019	  1.05%
 43	  38624	  0.81%
 44	  33512	  0.70%
 45	  38921	  0.82%
 46	  30735	  0.65%
 47	  23829	  0.50%
 48	   5396	  0.11%
 49	  16959	  0.36%
 50	 388815	  8.17%


criterion=sequence-density
sequence-density=7.98
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=19
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=3.22
fanout-score-rank=1
prefix-density=0.02
prefix-fanout=1.0
sequence=TGCAGGACACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG
                                 Started job on |	Mar 04 06:44:27
                             Started mapping on |	Mar 04 06:44:27
                                    Finished on |	Mar 04 06:45:02
       Mapping speed, Million of reads per hour |	502.38

                          Number of input reads |	4884255
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2552150
                        Uniquely mapped reads % |	52.25%
                          Average mapped length |	26.77
                       Number of splices: Total |	2085
            Number of splices: Annotated (sjdb) |	304
                       Number of splices: GT/AG |	1820
                       Number of splices: GC/AG |	25
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	240
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.50
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1867010
             % of reads mapped to multiple loci |	38.23%
        Number of reads mapped to too many loci |	106523
             % of reads mapped to too many loci |	2.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.77%
                     % of reads unmapped: other |	0.57%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	465095	465095	465095
N_multimapping	1867010	1867010	1867010
N_noFeature	112064	137051	1947818
N_ambiguous	1383638	876575	21974
UnstrandedReadsAssigned:1056448 PositiveStrandReadsAssigned:1538524 NegativeStrandReadsAssigned:582358
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030912 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030912-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,884,255 reads, 1,050,726 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 619 rounds

 109283 SRR5030912.ke.tsv
  52636 SRR5030912.se.tsv
 161919 total
==> SRR5030912.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030912.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030912 completed mapping pipeline successfully
