Starting /dee2/code/volunteer_pipeline.sh SRR5030933
    current disk space = 160620740608
    free memory = 73926180 
SRR5030933 SRAfilesize
5aa7b63ed756fa1a4ad776512a312c15  SRR5030933.sra
SRR5030933.sra file validated
SRR5030933 is single end
SRR5030933 is conventional basespace
SRR5030933 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45025	33.0	31.0	34.0	31.0	34.0
2	32.73625	34.0	31.0	34.0	31.0	34.0
3	32.8195	34.0	31.0	34.0	31.0	34.0
4	36.28275	37.0	37.0	37.0	35.0	37.0
5	36.3145	37.0	37.0	37.0	35.0	37.0
6	36.092	37.0	35.0	37.0	35.0	37.0
7	36.14225	37.0	35.0	37.0	35.0	37.0
8	36.19775	37.0	36.0	37.0	35.0	37.0
9	37.94775	39.0	38.0	39.0	35.0	39.0
10	38.0265	39.0	38.0	39.0	35.0	39.0
11	37.81025	39.0	38.0	39.0	35.0	39.0
12	37.91575	39.0	38.0	39.0	35.0	39.0
13	37.8585	39.0	38.0	39.0	35.0	39.0
14	39.3535	41.0	39.0	41.0	36.0	41.0
15	39.324	41.0	39.0	41.0	36.0	41.0
16	39.2145	40.0	39.0	41.0	36.0	41.0
17	39.4175	41.0	39.0	41.0	36.0	41.0
18	39.27525	41.0	39.0	41.0	36.0	41.0
19	38.98675	40.0	38.0	41.0	35.0	41.0
20	39.03525	40.0	39.0	41.0	35.0	41.0
21	39.16575	40.0	39.0	41.0	36.0	41.0
22	39.131	41.0	39.0	41.0	36.0	41.0
23	38.965	40.0	39.0	41.0	35.0	41.0
24	38.58225	40.0	38.0	41.0	34.0	41.0
25	38.86375	40.0	39.0	41.0	35.0	41.0
26	38.95575	40.0	39.0	41.0	35.0	41.0
27	38.90675	40.0	39.0	41.0	35.0	41.0
28	38.80625	40.0	38.0	41.0	35.0	41.0
29	38.07525	40.0	38.0	41.0	33.0	41.0
30	38.55725	40.0	38.0	41.0	34.0	41.0
31	38.7875	40.0	38.0	41.0	35.0	41.0
32	38.99475	40.0	39.0	41.0	35.0	41.0
33	38.2545	40.0	38.0	41.0	34.0	41.0
34	38.2345	40.0	38.0	41.0	33.0	41.0
35	38.08975	40.0	38.0	41.0	33.0	41.0
36	34.28375	38.0	31.0	40.0	23.0	41.0
37	35.6685	38.0	35.0	40.0	30.0	41.0
38	37.06	39.0	37.0	40.0	31.0	41.0
39	37.013	39.0	37.0	40.0	31.0	41.0
40	37.0745	39.0	37.0	41.0	31.0	41.0
41	37.2635	40.0	37.0	41.0	32.0	41.0
42	37.391	40.0	37.0	41.0	33.0	41.0
43	37.1645	40.0	37.0	41.0	32.0	41.0
44	36.9075	39.0	37.0	41.0	31.0	41.0
45	36.67425	39.0	36.0	40.0	31.0	41.0
46	34.95825	38.0	34.0	40.0	24.0	41.0
47	35.7855	39.0	35.0	40.0	28.0	41.0
48	36.15	39.0	36.0	41.0	30.0	41.0
49	35.00575	39.0	35.0	40.0	27.0	41.0
50	30.585	34.0	28.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	4.0
19	2.0
20	4.0
21	4.0
22	13.0
23	7.0
24	16.0
25	11.0
26	22.0
27	28.0
28	35.0
29	37.0
30	38.0
31	55.0
32	86.0
33	116.0
34	154.0
35	190.0
36	320.0
37	490.0
38	1006.0
39	1360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.712140175219023	21.25156445556946	53.41677096370463	15.619524405506885
2	23.025000000000002	26.400000000000002	21.575	28.999999999999996
3	18.725	18.65	20.175	42.449999999999996
4	27.35	33.0	14.899999999999999	24.75
5	20.849999999999998	38.550000000000004	27.500000000000004	13.100000000000001
6	34.599999999999994	15.425	37.375	12.6
7	16.825000000000003	24.474999999999998	24.125	34.575
8	24.6	37.574999999999996	15.275	22.55
9	21.65	36.25	29.5	12.6
10	21.6	21.0	27.775	29.625
11	30.425	13.850000000000001	15.1	40.625
12	14.799999999999999	17.724999999999998	26.200000000000003	41.275
13	21.525	20.974999999999998	36.05	21.45
14	33.95	26.075	25.474999999999998	14.499999999999998
15	37.409352338084524	22.655663915978995	26.70667666916729	13.228307076769191
16	14.649999999999999	26.125	21.175	38.05
17	23.674999999999997	24.85	37.025000000000006	14.45
18	25.85	18.85	29.7	25.6
19	37.3	14.35	30.425	17.925
20	46.85	16.900000000000002	24.175	12.075
21	22.8	23.425	24.525	29.25
22	30.5	13.325000000000001	48.1	8.075000000000001
23	25.224999999999998	13.4	47.3	14.075
24	51.125	14.000000000000002	27.025	7.85
25	53.2	23.05	14.799999999999999	8.95
26	28.782195548887223	47.23680920230057	12.828207051762941	11.152788197049263
27	13.700000000000001	51.449999999999996	23.599999999999998	11.25
28	9.775	25.5	49.575	15.15
29	10.575	11.525	59.25	18.65
30	9.75	6.65	37.1	46.5
31	14.099999999999998	4.8500000000000005	46.075	34.975
32	26.875	5.975	23.75	43.4
33	54.400000000000006	8.075000000000001	12.950000000000001	24.575
34	64.3	7.049999999999999	15.2	13.450000000000001
35	65.275	7.000000000000001	17.875	9.85
36	44.574999999999996	7.925	35.15	12.35
37	43.425000000000004	11.525	22.55	22.5
38	24.125	14.799999999999999	11.575000000000001	49.5
39	14.374999999999998	23.400000000000002	9.75	52.47500000000001
40	14.399999999999999	48.4	10.625	26.575
41	23.375	53.2	8.525	14.899999999999999
42	51.24999999999999	30.599999999999998	10.274999999999999	7.875
43	54.05	27.400000000000002	8.5	10.05
44	30.85	48.325	8.0	12.825000000000001
45	16.125	53.725	11.924999999999999	18.224999999999998
46	11.450000000000001	28.075	18.125	42.35
47	9.025	13.8	40.475	36.7
48	10.674999999999999	12.025	22.025	55.275
49	12.65	19.35	12.049999999999999	55.95
50	18.575	38.125	11.65	31.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	3.5
28	5.0
29	3.5
30	2.0
31	6.0
32	10.0
33	17.5
34	25.0
35	30.5
36	36.0
37	50.5
38	65.0
39	56.0
40	47.0
41	52.0
42	57.0
43	100.0
44	143.0
45	196.5
46	250.0
47	366.0
48	482.0
49	534.5
50	587.0
51	744.0
52	901.0
53	626.0
54	351.0
55	372.5
56	394.0
57	360.0
58	326.0
59	201.0
60	76.0
61	59.0
62	42.0
63	71.5
64	101.0
65	60.5
66	20.0
67	14.0
68	8.0
69	15.0
70	22.0
71	27.0
72	32.0
73	19.5
74	7.0
75	4.0
76	1.0
77	1.5
78	2.0
79	2.0
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53940886699507	31.075000000000003
2	9.298029556650246	7.55
3	3.5098522167487682	4.275
4	2.832512315270936	4.6
5	1.5394088669950738	3.125
6	0.9236453201970444	2.25
7	0.6157635467980296	1.7500000000000002
8	0.8620689655172413	2.8000000000000003
9	0.3694581280788177	1.35
>10	3.140394088669951	24.4
>50	0.18472906403940886	5.525
>100	0.18472906403940886	11.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	183	4.575	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	121	3.025	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	99	2.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	63	1.575	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	59	1.4749999999999999	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	50	1.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	46	1.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	38	0.95	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	31	0.775	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	16	0.4	RNA PCR Primer, Index 7 (100% over 35bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	16	0.4	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	13	0.325	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	13	0.325	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	12	0.3	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 33bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	11	0.27499999999999997	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC	9	0.22499999999999998	RNA PCR Primer, Index 32 (100% over 44bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	8	0.2	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	8	0.2	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	8	0.2	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	6	0.15	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	5	0.125	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	5	0.125	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.1	0.0	0.0	0.0
2	0.0	0.1	0.0	0.0	0.0
3	0.0	0.15	0.0	0.0	0.0
4	0.0	0.15	0.0	0.0	0.0
5	0.0	0.15	0.0	0.0	0.0
6	0.0	0.175	0.0	0.0	0.0
7	0.0	0.425	0.0	0.0	0.0
8	0.0	0.625	0.0	0.0	0.0
9	0.0	0.675	0.0	0.0	0.0
10	0.0	0.725	0.0	0.0	0.0
11	0.0	0.9	0.0	0.0	0.0
12	0.0	1.025	0.0	0.0	0.0
13	0.0	1.225	0.0	0.0	0.0
14	0.0	1.475	0.0	0.0	0.0
15	0.0	1.8	0.0	0.0	0.0
16	0.0	2.7	0.0	0.0	0.0
17	0.0	3.15	0.0	0.0	0.0
18	0.0	3.9	0.0	0.0	0.0
19	0.0	4.525	0.0	0.0	0.0
20	0.0	5.725	0.0	0.0	0.0
21	0.0	10.55	0.0	0.0	0.0
22	0.0	21.35	0.0	0.0	0.0
23	0.0	52.125	0.0	0.0	0.0
24	0.0	66.85	0.0	0.0	0.0
25	0.0	71.825	0.0	0.0	0.0
26	0.0	72.575	0.0	0.0	0.0
27	0.0	72.95	0.0	0.0	0.0
28	0.0	73.525	0.0	0.0	0.0
29	0.0	73.975	0.0	0.0	0.0
30	0.0	74.325	0.0	0.0	0.0
31	0.0	75.2	0.0	0.0	0.0
32	0.0	76.025	0.0	0.0	0.0
33	0.0	76.8	0.0	0.0	0.0
34	0.0	79.6	0.0	0.0	0.0
35	0.0	80.625	0.0	0.0	0.0
36	0.0	81.125	0.0	0.0	0.0
37	0.0	81.85	0.0	0.0	0.0
38	0.0	82.1	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	40	5.315087E-9	44.000004	2
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AATCCAG	20	6.952051E-4	44.000004	8
ATCCAGG	20	6.952051E-4	44.000004	9
CTTTAAC	40	5.315087E-9	44.000004	12
ACTGAGC	20	6.952051E-4	44.000004	7
TTCAAGT	20	6.952051E-4	44.000004	1
GTACTGA	20	6.952051E-4	44.000004	5
AGGATAG	20	6.952051E-4	44.000004	13
CCTGTAC	20	6.952051E-4	44.000004	2
ATAGGCT	20	6.952051E-4	44.000004	16
GAGCTGC	20	6.952051E-4	44.000004	10
CCTGAGA	40	5.315087E-9	44.000004	3
CATTCAA	20	6.952051E-4	44.000004	3
ACATTCA	20	6.952051E-4	44.000004	2
GGATAGG	20	6.952051E-4	44.000004	14
TGCCCCG	20	6.952051E-4	44.000004	14
TAACCTG	40	5.315087E-9	44.000004	15
GCTGCCC	20	6.952051E-4	44.000004	12
>>END_MODULE
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292551 spots for SRR5030933.sra
Written 292551 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
Read 292548 spots for SRR5030933.sra
Written 292548 spots for SRR5030933.sra
SRR ids: ['SRR5030933.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_malisot0
SRR5030933.sra spots: 3510579
blocks: [[1, 292548], [292549, 585096], [585097, 877644], [877645, 1170192], [1170193, 1462740], [1462741, 1755288], [1755289, 2047836], [2047837, 2340384], [2340385, 2632932], [2632933, 2925480], [2925481, 3218028], [3218029, 3510579]]
SRR5030933 file size 607386
SRR5030933 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030933 SRR5030933_1.fastq
Input file:	SRR5030933_1.fastq
trimmed:	SRR5030933-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:48:23 2020 >> started

Wed Mar  4 06:48:25 2020 >> done (2.037s)
3510579 reads processed; of these:
    118 ( 0.00%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
3510458 (100.00%) reads available; of these:
 198286 ( 5.65%) trimmed reads available after processing
3312172 (94.35%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     36	  0.00%
 19	     62	  0.00%
 20	    128	  0.00%
 21	    154	  0.00%
 22	    330	  0.01%
 23	    345	  0.01%
 24	    371	  0.01%
 25	    477	  0.01%
 26	    389	  0.01%
 27	    566	  0.02%
 28	    697	  0.02%
 29	   1166	  0.03%
 30	    756	  0.02%
 31	    889	  0.03%
 32	   1146	  0.03%
 33	   1398	  0.04%
 34	   2729	  0.08%
 35	   2926	  0.08%
 36	   2115	  0.06%
 37	   2368	  0.07%
 38	   2697	  0.08%
 39	   2926	  0.08%
 40	   3106	  0.09%
 41	   3887	  0.11%
 42	   4701	  0.13%
 43	   6525	  0.19%
 44	  11418	  0.33%
 45	  13733	  0.39%
 46	  12849	  0.37%
 47	  19117	  0.54%
 48	  41255	  1.18%
 49	  57024	  1.62%
 50	3312172	 94.35%
3510458 reads passed initial QC


criterion=sequence-density
sequence-density=82.06
sequence-density-rank=1
fanout-score=19.60
fanout-score-rank=1
prefix-density=84.01
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC


criterion=fanout-score
sequence-density=82.06
sequence-density-rank=1
fanout-score=19.60
fanout-score-rank=1
prefix-density=84.01
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC -o SRR5030933 -
Input file:	STDIN
trimmed:	SRR5030933-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:48:41 2020 >> started

Wed Mar  4 06:48:47 2020 >> done (5.368s)
3425869 reads processed; of these:
 147906 ( 4.32%) short reads filtered out after trimming by size control
   4968 ( 0.15%) empty reads filtered out after trimming by size control
3272995 (95.54%) reads available; of these:
2923180 (89.31%) trimmed reads available after processing
 349815 (10.69%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  20718	  0.63%
 19	  38870	  1.19%
 20	 163596	  5.00%
 21	 368576	 11.26%
 22	1051153	 32.12%
 23	 508812	 15.55%
 24	 183695	  5.61%
 25	  35160	  1.07%
 26	  18735	  0.57%
 27	  18847	  0.58%
 28	  19547	  0.60%
 29	  15064	  0.46%
 30	  26145	  0.80%
 31	  30140	  0.92%
 32	  34951	  1.07%
 33	  86988	  2.66%
 34	  33428	  1.02%
 35	  21895	  0.67%
 36	  23200	  0.71%
 37	  15073	  0.46%
 38	  17510	  0.53%
 39	  16650	  0.51%
 40	  22810	  0.70%
 41	  30382	  0.93%
 42	  31161	  0.95%
 43	  25322	  0.77%
 44	  22463	  0.69%
 45	  26090	  0.80%
 46	  20917	  0.64%
 47	  18140	  0.55%
 48	   3711	  0.11%
 49	  12401	  0.38%
 50	 310845	  9.50%


criterion=sequence-density
sequence-density=8.30
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.47
sequence-density-rank=31
fanout-score=15.39
fanout-score-rank=1
prefix-density=7.26
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:49:09
                             Started mapping on |	Mar 04 06:49:09
                                    Finished on |	Mar 04 06:49:35
       Mapping speed, Million of reads per hour |	464.90

                          Number of input reads |	3357584
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1734364
                        Uniquely mapped reads % |	51.66%
                          Average mapped length |	26.93
                       Number of splices: Total |	1589
            Number of splices: Annotated (sjdb) |	227
                       Number of splices: GT/AG |	1408
                       Number of splices: GC/AG |	19
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	162
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.55
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1205302
             % of reads mapped to multiple loci |	35.90%
        Number of reads mapped to too many loci |	98616
             % of reads mapped to too many loci |	2.94%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.40%
                     % of reads unmapped: other |	1.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	417918	417918	417918
N_multimapping	1205302	1205302	1205302
N_noFeature	71024	88047	1309419
N_ambiguous	928506	565139	17445
UnstrandedReadsAssigned:734834 PositiveStrandReadsAssigned:1081178 NegativeStrandReadsAssigned:407500
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030933 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030933-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,357,584 reads, 758,703 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 681 rounds

 109283 SRR5030933.ke.tsv
  52636 SRR5030933.se.tsv
 161919 total
==> SRR5030933.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030933.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030933 completed mapping pipeline successfully
