Starting /dee2/code/volunteer_pipeline.sh SRR5031101
    current disk space = 160475217920
    free memory = 73910752 
SRR5031101 SRAfilesize
c641c71133cd4819ab4b2fba7f7a2445  SRR5031101.sra
SRR5031101.sra file validated
SRR5031101 is single end
SRR5031101 is conventional basespace
SRR5031101 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95125	31.0	31.0	34.0	30.0	34.0
2	32.3985	33.0	31.0	34.0	31.0	34.0
3	32.347	33.0	31.0	34.0	31.0	34.0
4	36.10825	37.0	35.0	37.0	35.0	37.0
5	36.091	37.0	35.0	37.0	35.0	37.0
6	35.8775	37.0	35.0	37.0	35.0	37.0
7	35.65625	37.0	35.0	37.0	35.0	37.0
8	35.4965	37.0	35.0	37.0	33.0	37.0
9	36.73575	39.0	37.0	39.0	32.0	39.0
10	36.0725	39.0	35.0	39.0	30.0	39.0
11	37.3275	39.0	37.0	39.0	34.0	39.0
12	37.62375	39.0	37.0	39.0	35.0	39.0
13	37.51175	39.0	37.0	39.0	35.0	39.0
14	38.41	40.0	38.0	41.0	34.0	41.0
15	36.74925	39.0	36.0	41.0	29.0	41.0
16	36.75975	38.0	36.0	40.0	31.0	41.0
17	36.70675	39.0	36.0	40.0	30.0	41.0
18	38.32475	40.0	37.0	41.0	34.0	41.0
19	38.842	40.0	38.0	41.0	36.0	41.0
20	38.52325	40.0	38.0	41.0	35.0	41.0
21	38.70325	40.0	38.0	41.0	35.0	41.0
22	37.62875	40.0	37.0	41.0	32.0	41.0
23	38.2305	39.0	38.0	41.0	34.0	41.0
24	37.593	40.0	37.0	41.0	32.0	41.0
25	38.25225	40.0	38.0	41.0	34.0	41.0
26	37.97875	40.0	38.0	41.0	34.0	41.0
27	38.406	40.0	38.0	41.0	34.0	41.0
28	38.681	40.0	38.0	41.0	34.0	41.0
29	38.592	40.0	38.0	41.0	35.0	41.0
30	38.9175	40.0	38.0	41.0	35.0	41.0
31	38.98525	40.0	38.0	41.0	35.0	41.0
32	39.04125	40.0	38.0	41.0	36.0	41.0
33	39.057	40.0	39.0	41.0	35.0	41.0
34	38.331	40.0	38.0	41.0	33.0	41.0
35	37.31225	40.0	36.0	41.0	30.0	41.0
36	32.799	35.0	30.0	39.0	23.0	41.0
37	34.94925	37.0	33.0	39.0	30.0	40.0
38	36.2925	38.0	35.0	39.0	30.0	40.0
39	36.638	38.0	36.0	40.0	31.0	40.0
40	37.1915	39.0	37.0	40.0	32.0	41.0
41	37.599	40.0	37.0	40.0	33.0	41.0
42	37.674	40.0	37.0	41.0	33.0	41.0
43	37.60875	40.0	37.0	41.0	33.0	41.0
44	36.69825	39.0	36.0	40.0	30.0	41.0
45	37.13025	39.0	37.0	40.0	32.0	41.0
46	37.27925	40.0	37.0	40.0	32.0	41.0
47	36.4255	39.0	35.0	40.0	30.0	41.0
48	37.30325	39.0	37.0	40.0	32.0	41.0
49	37.248	40.0	37.0	41.0	32.0	41.0
50	35.22825	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.301116838487971
1101	2	-0.12199312714776056
1101	3	-0.16451890034363714
1101	4	-0.08891752577319068
1101	5	-0.20661512027491824
1101	6	-0.36254295532646097
1101	7	-0.5541237113402033
1101	8	-0.4673539518900327
1101	9	-0.579467353951884
1101	10	-0.4033505154639201
1101	11	-0.2951030927835063
1101	12	-0.6108247422680435
1101	13	0.11168384879724869
1101	14	-0.006013745704464668
1101	15	1.0060137457044647
1101	16	1.6207044673539457
1101	17	-0.14518900343642827
1101	18	0.1387457044673539
1101	19	-0.01288659793814162
1101	20	-0.08075601374569885
1101	21	-0.6683848797250818
1101	22	-0.2031786941580691
1101	23	-0.4445876288659818
1101	24	-0.47379725085910707
1101	25	0.7719072164948457
1101	26	-0.24656357388316508
1101	27	-0.4991408934707948
1101	28	-0.7573024054982795
1101	29	0.28006872852233755
1101	30	-0.3479381443298948
1101	31	-0.3251718213058439
1101	32	0.43384879725086023
1101	33	-0.4630584192439926
1101	34	-0.8939003436426134
1101	35	0.21520618556700555
1101	36	-0.5322164948453647
1101	37	-0.929553264604813
1101	38	-0.9798109965635717
1101	39	-0.7225085910652922
1101	40	0.33591065292095834
1101	41	0.46907216494845017
1101	42	-0.3560996563573866
1101	43	-0.4347079037800654
1101	44	-1.0446735395188966
1101	45	-1.212628865979383
1101	46	-1.4733676975945045
1101	47	-1.6116838487972487
1101	48	-0.9390034364261197
1101	49	-1.2384020618556733
1101	50	-1.5609965635738803
1102	1	0.301116838487971
1102	2	0.12199312714776767
1102	3	0.16451890034364425
1102	4	0.08891752577319778
1102	5	0.20661512027491113
1102	6	0.36254295532646097
1102	7	0.5541237113402104
1102	8	0.4673539518900327
1102	9	0.5794673539518911
1102	10	0.403350515463913
1102	11	0.2951030927835063
1102	12	0.6108247422680364
1102	13	-0.11168384879724869
1102	14	0.006013745704464668
1102	15	-1.0060137457044647
1102	16	-1.6207044673539528
1102	17	0.14518900343642116
1102	18	-0.1387457044673539
1102	19	0.012886597938148725
1102	20	0.08075601374570596
1102	21	0.6683848797250889
1102	22	0.2031786941580762
1102	23	0.4445876288659747
1102	24	0.47379725085910707
1102	25	-0.7719072164948457
1102	26	0.24656357388315797
1102	27	0.4991408934707877
1102	28	0.7573024054982795
1102	29	-0.28006872852233755
1102	30	0.3479381443299019
1102	31	0.3251718213058439
1102	32	-0.4338487972508531
1102	33	0.4630584192439855
1102	34	0.8939003436426063
1102	35	-0.21520618556701265
1102	36	0.5322164948453576
1102	37	0.929553264604813
1102	38	0.9798109965635788
1102	39	0.7225085910652922
1102	40	-0.33591065292096545
1102	41	-0.46907216494845727
1102	42	0.3560996563573937
1102	43	0.4347079037800725
1102	44	1.0446735395189037
1102	45	1.212628865979383
1102	46	1.4733676975945045
1102	47	1.6116838487972487
1102	48	0.9390034364261126
1102	49	1.2384020618556661
1102	50	1.5609965635738874
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	2.0
20	4.0
21	4.0
22	3.0
23	8.0
24	3.0
25	10.0
26	6.0
27	15.0
28	20.0
29	32.0
30	36.0
31	79.0
32	93.0
33	138.0
34	184.0
35	288.0
36	435.0
37	781.0
38	1218.0
39	638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.8999999999999995	24.825	59.0	10.274999999999999
2	27.05205205205205	27.602602602602605	17.792792792792792	27.55255255255255
3	22.525000000000002	26.85	19.25	31.374999999999996
4	34.5	29.525000000000002	11.575000000000001	24.4
5	24.8	38.4	28.249999999999996	8.55
6	33.900000000000006	15.675	38.125	12.3
7	12.725	25.55	23.799999999999997	37.925
8	30.375000000000004	28.449999999999996	14.149999999999999	27.025
9	17.549999999999997	38.15	32.25	12.049999999999999
10	19.35	24.3	26.0	30.349999999999998
11	37.3	6.7250000000000005	16.25	39.725
12	15.425	18.8	30.049999999999997	35.725
13	29.9	16.175	37.625	16.3
14	35.35	26.825	26.700000000000003	11.125
15	40.625	16.575	26.674999999999997	16.125
16	16.875	17.150000000000002	26.1	39.875
17	23.724999999999998	23.875	38.574999999999996	13.825000000000001
18	26.924999999999997	17.325	29.175	26.575
19	32.0	17.1	35.6	15.299999999999999
20	49.975	12.125	26.575	11.325000000000001
21	21.425	19.725	33.775	25.074999999999996
22	28.000000000000004	8.575000000000001	54.925000000000004	8.5
23	27.150000000000002	12.125	50.0	10.725
24	56.35	10.125	26.150000000000002	7.375
25	54.400000000000006	24.85	14.549999999999999	6.2
26	26.724999999999998	53.349999999999994	12.3	7.625
27	13.25	52.25	26.400000000000002	8.1
28	8.575000000000001	24.2	55.325	11.899999999999999
29	9.325	9.35	60.12499999999999	21.2
30	8.35	4.65	39.7	47.3
31	11.924999999999999	3.85	48.15	36.075
32	28.1	4.475	20.225	47.199999999999996
33	60.925	5.949999999999999	12.475	20.65
34	70.39999999999999	5.975	11.924999999999999	11.700000000000001
35	69.125	4.675	18.925	7.2749999999999995
36	45.074999999999996	5.3	38.35	11.275
37	49.625	7.95	17.65	24.775
38	20.974999999999998	14.475	10.549999999999999	54.0
39	12.275	26.025	7.7	54.0
40	12.5	55.7	6.875000000000001	24.925
41	25.4	53.6	6.425	14.575
42	54.675	30.325000000000003	5.575	9.425
43	54.275	32.725	4.95	8.05
44	27.775	55.60000000000001	3.5000000000000004	13.125
45	15.1	53.925	10.925	20.05
46	8.05	24.425	19.85	47.675
47	5.949999999999999	14.099999999999998	40.525	39.425
48	5.925	11.600000000000001	19.0	63.475
49	11.175	21.925	10.225	56.675
50	18.45	41.099999999999994	10.225	30.225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	2.0
32	4.0
33	4.0
34	4.0
35	5.0
36	6.0
37	8.0
38	10.0
39	14.0
40	18.0
41	23.0
42	28.0
43	100.0
44	172.0
45	208.0
46	244.0
47	355.0
48	466.0
49	594.0
50	722.0
51	865.5
52	1009.0
53	720.0
54	431.0
55	426.5
56	422.0
57	325.0
58	228.0
59	157.0
60	86.0
61	60.5
62	35.0
63	41.5
64	48.0
65	40.0
66	32.0
67	20.5
68	9.0
69	8.5
70	8.0
71	11.5
72	15.0
73	8.5
74	2.0
75	1.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.724999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7455268389662	28.575
2	8.614976805831677	6.5
3	4.307488402915839	4.875
4	2.8495692511597084	4.3
5	2.319416832339298	4.375
6	0.7952286282306162	1.7999999999999998
7	0.7952286282306162	2.1
8	0.5964214711729622	1.7999999999999998
9	0.33134526176275675	1.125
>10	3.0483764082173623	21.5
>50	0.33134526176275675	10.225
>100	0.26507620941020543	12.825000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	150	3.75	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	130	3.25	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	114	2.85	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	99	2.475	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	84	2.1	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	79	1.975	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	64	1.6	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	48	1.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC	31	0.775	RNA PCR Primer, Index 25 (100% over 50bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTCGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC	23	0.575	RNA PCR Primer, Index 5 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	23	0.575	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGACCCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTCTAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC	11	0.27499999999999997	RNA PCR Primer, Index 5 (100% over 35bp)
AAGCTGCCAGTTGACGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (96% over 30bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	10	0.25	No Hit
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	8	0.2	RNA PCR Primer, Index 1 (100% over 34bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTACCCTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGACCCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTCCTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTCTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCCGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	6	0.15	No Hit
TTCAAGTAATCCCGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT	6	0.15	RNA PCR Primer, Index 8 (100% over 36bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC	5	0.125	RNA PCR Primer, Index 25 (100% over 44bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTACCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGCATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTCTGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	5	0.125	No Hit
AACATTCATTGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.15	0.0	0.0	0.0
2	0.0	1.175	0.0	0.0	0.0
3	0.0	1.3	0.0	0.0	0.0
4	0.0	1.3	0.0	0.0	0.0
5	0.0	1.375	0.0	0.0	0.0
6	0.0	1.45	0.0	0.0	0.0
7	0.0	1.65	0.0	0.0	0.0
8	0.0	1.725	0.0	0.0	0.0
9	0.0	1.85	0.0	0.0	0.0
10	0.0	2.2	0.0	0.0	0.0
11	0.0	2.475	0.0	0.0	0.0
12	0.0	2.8	0.0	0.0	0.0
13	0.0	3.15	0.0	0.0	0.0
14	0.0	3.375	0.0	0.0	0.0
15	0.0	3.75	0.0	0.0	0.0
16	0.0	4.975	0.0	0.0	0.0
17	0.0	5.625	0.0	0.0	0.0
18	0.0	6.525	0.0	0.0	0.0
19	0.0	7.05	0.0	0.0	0.0
20	0.0	8.15	0.0	0.0	0.0
21	0.0	13.275	0.0	0.0	0.0
22	0.0	28.45	0.0	0.0	0.0
23	0.0	63.575	0.0	0.0	0.0
24	0.0	77.75	0.0	0.0	0.0
25	0.0	83.75	0.0	0.0	0.0
26	0.0	85.025	0.0	0.0	0.0
27	0.0	85.825	0.0	0.0	0.0
28	0.0	86.425	0.0	0.0	0.0
29	0.0	86.875	0.0	0.0	0.0
30	0.0	87.375	0.0	0.0	0.0
31	0.0	87.825	0.0	0.0	0.0
32	0.0	88.6	0.0	0.0	0.0
33	0.0	89.7	0.0	0.0	0.0
34	0.0	91.45	0.0	0.0	0.0
35	0.0	92.3	0.0	0.0	0.0
36	0.0	92.6	0.0	0.0	0.0
37	0.0	93.05	0.0	0.0	0.0
38	0.0	93.175	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTGA	20	6.952051E-4	44.000004	8
CTGAGCT	40	5.315087E-9	44.000004	11
TTGTATA	20	6.952051E-4	44.000004	13
GAGCTTG	40	5.315087E-9	44.000004	13
ACTCCTT	20	6.952051E-4	44.000004	17
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
TCGGATC	40	5.315087E-9	44.000004	1
CGGATCC	40	5.315087E-9	44.000004	2
ACATTCA	45	2.728484E-10	44.000004	2
GTATAGT	20	6.952051E-4	44.000004	15
ATAGTTT	20	6.952051E-4	44.000004	17
GCTGCCA	20	6.952051E-4	44.000004	3
GCTGTCG	40	5.315087E-9	44.000004	11
GATCCGT	40	5.315087E-9	44.000004	4
AGGCTTG	20	6.952051E-4	44.000004	18
GGTAGTA	20	6.952051E-4	44.000004	4
TCCGTCT	40	5.315087E-9	44.000004	6
ATCCGTC	40	5.315087E-9	44.000004	5
CTGTCGG	40	5.315087E-9	44.000004	12
>>END_MODULE
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49066 spots for SRR5031101.sra
Written 49066 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
Read 49062 spots for SRR5031101.sra
Written 49062 spots for SRR5031101.sra
SRR ids: ['SRR5031101.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_izlxclu6
SRR5031101.sra spots: 588748
blocks: [[1, 49062], [49063, 98124], [98125, 147186], [147187, 196248], [196249, 245310], [245311, 294372], [294373, 343434], [343435, 392496], [392497, 441558], [441559, 490620], [490621, 539682], [539683, 588748]]
SRR5031101 file size 101370
SRR5031101 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031101 SRR5031101_1.fastq
Input file:	SRR5031101_1.fastq
trimmed:	SRR5031101-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:24:12 2020 >> started

Wed Mar  4 07:24:14 2020 >> done (1.729s)
588748 reads processed; of these:
    35 ( 0.01%) short reads filtered out after trimming by size control
     2 ( 0.00%) empty reads filtered out after trimming by size control
588711 (99.99%) reads available; of these:
 47698 ( 8.10%) trimmed reads available after processing
541013 (91.90%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    12	  0.00%
 19	    13	  0.00%
 20	    30	  0.01%
 21	    59	  0.01%
 22	    70	  0.01%
 23	    87	  0.01%
 24	    70	  0.01%
 25	   129	  0.02%
 26	    91	  0.02%
 27	   106	  0.02%
 28	   135	  0.02%
 29	   198	  0.03%
 30	   108	  0.02%
 31	   178	  0.03%
 32	   232	  0.04%
 33	   489	  0.08%
 34	   691	  0.12%
 35	  1806	  0.31%
 36	  1017	  0.17%
 37	   919	  0.16%
 38	   781	  0.13%
 39	   803	  0.14%
 40	   977	  0.17%
 41	  1086	  0.18%
 42	  1268	  0.22%
 43	  2099	  0.36%
 44	  2161	  0.37%
 45	  3100	  0.53%
 46	  3738	  0.63%
 47	  3518	  0.60%
 48	 11585	  1.97%
 49	 10142	  1.72%
 50	541013	 91.90%
588711 reads passed initial QC


criterion=sequence-density
sequence-density=93.27
sequence-density-rank=1
fanout-score=19.29
fanout-score-rank=2
prefix-density=93.38
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC


criterion=fanout-score
sequence-density=4.11
sequence-density-rank=2
fanout-score=59.64
fanout-score-rank=1
prefix-density=93.53
prefix-fanout=2.6
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC -o SRR5031101 -
Input file:	STDIN
trimmed:	SRR5031101-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:24:23 2020 >> started

Wed Mar  4 07:24:25 2020 >> done (1.869s)
576185 reads processed; of these:
 31298 ( 5.43%) short reads filtered out after trimming by size control
  6736 ( 1.17%) empty reads filtered out after trimming by size control
538151 (93.40%) reads available; of these:
527994 (98.11%) trimmed reads available after processing
 10157 ( 1.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  3277	  0.61%
 19	  7107	  1.32%
 20	 33970	  6.31%
 21	 85635	 15.91%
 22	209316	 38.90%
 23	 89911	 16.71%
 24	 36386	  6.76%
 25	  8518	  1.58%
 26	  3974	  0.74%
 27	  3268	  0.61%
 28	  3048	  0.57%
 29	  2507	  0.47%
 30	  3500	  0.65%
 31	  4073	  0.76%
 32	  5292	  0.98%
 33	  9742	  1.81%
 34	  3160	  0.59%
 35	  2074	  0.39%
 36	  2294	  0.43%
 37	  1219	  0.23%
 38	  1168	  0.22%
 39	  1024	  0.19%
 40	  1425	  0.26%
 41	  1669	  0.31%
 42	  1801	  0.33%
 43	  1112	  0.21%
 44	   882	  0.16%
 45	  1170	  0.22%
 46	   781	  0.15%
 47	   613	  0.11%
 48	   133	  0.02%
 49	   151	  0.03%
 50	  7951	  1.48%


criterion=sequence-density
sequence-density=9.98
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.22
sequence-density-rank=9
fanout-score=13.91
fanout-score-rank=1
prefix-density=1.90
prefix-fanout=13.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC
                                 Started job on |	Mar 04 07:24:50
                             Started mapping on |	Mar 04 07:24:51
                                    Finished on |	Mar 04 07:25:18
       Mapping speed, Million of reads per hour |	73.42

                          Number of input reads |	550677
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	276683
                        Uniquely mapped reads % |	50.24%
                          Average mapped length |	22.81
                       Number of splices: Total |	949
            Number of splices: Annotated (sjdb) |	206
                       Number of splices: GT/AG |	932
                       Number of splices: GC/AG |	10
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	6
                      Mismatch rate per base, % |	0.33%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.56
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	233320
             % of reads mapped to multiple loci |	42.37%
        Number of reads mapped to too many loci |	6305
             % of reads mapped to too many loci |	1.14%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.89%
                     % of reads unmapped: other |	0.35%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	40674	40674	40674
N_multimapping	233320	233320	233320
N_noFeature	14840	16573	194930
N_ambiguous	154194	80818	1209
UnstrandedReadsAssigned:107649 PositiveStrandReadsAssigned:179292 NegativeStrandReadsAssigned:80544
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031101 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031101-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 550,677 reads, 130,576 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 892 rounds

 109283 SRR5031101.ke.tsv
  52636 SRR5031101.se.tsv
 161919 total
==> SRR5031101.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031101.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031101 completed mapping pipeline successfully
