Starting /dee2/code/volunteer_pipeline.sh SRR5030954
    current disk space = 160605126656
    free memory = 73913184 
SRR5030954 SRAfilesize
bc07a3301064e5c10782a06b6c59c7ee  SRR5030954.sra
SRR5030954.sra file validated
SRR5030954 is single end
SRR5030954 is conventional basespace
SRR5030954 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40475	33.0	31.0	34.0	31.0	34.0
2	32.6785	34.0	31.0	34.0	31.0	34.0
3	32.77975	34.0	31.0	34.0	31.0	34.0
4	36.20725	37.0	37.0	37.0	35.0	37.0
5	36.2575	37.0	37.0	37.0	35.0	37.0
6	36.08425	37.0	35.0	37.0	35.0	37.0
7	36.0805	37.0	35.0	37.0	35.0	37.0
8	36.1125	37.0	35.0	37.0	35.0	37.0
9	37.89025	39.0	38.0	39.0	35.0	39.0
10	37.88775	39.0	38.0	39.0	35.0	39.0
11	37.74275	39.0	38.0	39.0	35.0	39.0
12	37.59325	39.0	38.0	39.0	35.0	39.0
13	37.69525	39.0	37.0	39.0	35.0	39.0
14	39.07975	40.0	39.0	41.0	36.0	41.0
15	39.216	41.0	39.0	41.0	36.0	41.0
16	39.0645	40.0	38.0	41.0	35.0	41.0
17	39.145	40.0	38.0	41.0	36.0	41.0
18	39.30375	41.0	39.0	41.0	36.0	41.0
19	39.269	41.0	39.0	41.0	36.0	41.0
20	39.141	41.0	39.0	41.0	36.0	41.0
21	39.0615	40.0	39.0	41.0	36.0	41.0
22	39.217	41.0	39.0	41.0	36.0	41.0
23	39.215	41.0	39.0	41.0	36.0	41.0
24	38.432	40.0	38.0	41.0	34.0	41.0
25	38.5865	40.0	38.0	41.0	34.0	41.0
26	38.778	40.0	38.0	41.0	35.0	41.0
27	38.8035	40.0	38.0	41.0	35.0	41.0
28	38.5805	40.0	38.0	41.0	34.0	41.0
29	37.9685	40.0	38.0	41.0	33.0	41.0
30	38.2135	40.0	38.0	41.0	33.0	41.0
31	38.757	40.0	38.0	41.0	35.0	41.0
32	38.785	40.0	38.0	41.0	35.0	41.0
33	37.67575	40.0	38.0	41.0	33.0	41.0
34	37.5035	40.0	37.0	41.0	31.0	41.0
35	37.29725	39.0	37.0	41.0	32.0	41.0
36	32.993	38.0	30.0	40.0	16.0	41.0
37	35.34925	38.0	33.0	40.0	28.0	41.0
38	36.83075	39.0	36.0	40.0	31.0	41.0
39	36.9555	39.0	36.0	40.0	31.0	41.0
40	36.9655	39.0	37.0	40.0	31.0	41.0
41	36.72975	39.0	36.0	40.0	31.0	41.0
42	36.5795	39.0	36.0	40.0	31.0	41.0
43	36.65275	39.0	36.0	40.0	30.0	41.0
44	36.868	39.0	37.0	41.0	31.0	41.0
45	36.347	39.0	36.0	40.0	30.0	41.0
46	33.97625	38.0	33.0	40.0	23.0	41.0
47	35.00175	38.0	34.0	40.0	26.0	41.0
48	35.29425	38.0	35.0	40.0	28.0	41.0
49	34.60875	38.0	34.0	40.0	27.0	41.0
50	30.52775	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	0.0
16	1.0
17	2.0
18	5.0
19	4.0
20	3.0
21	10.0
22	8.0
23	8.0
24	11.0
25	20.0
26	24.0
27	30.0
28	32.0
29	41.0
30	50.0
31	63.0
32	106.0
33	129.0
34	167.0
35	249.0
36	282.0
37	526.0
38	1063.0
39	1163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.782401604412133	26.34745550263224	45.926297317623465	15.943845575332164
2	20.65	27.275	20.95	31.125000000000004
3	25.45	17.95	21.025	35.575
4	31.075000000000003	30.675	14.499999999999998	23.75
5	20.525	39.900000000000006	24.2	15.375
6	28.575	16.35	39.675	15.4
7	21.15	20.05	22.575	36.225
8	21.55	32.375	16.950000000000003	29.125
9	24.575	32.725	27.425	15.275
10	25.55	18.025	30.975	25.45
11	30.575000000000003	17.675	15.225	36.525
12	15.325	20.775	32.375	31.525
13	26.75	20.05	30.625000000000004	22.575
14	27.6	32.1	21.55	18.75
15	35.525	23.075000000000003	24.75	16.650000000000002
16	17.424999999999997	23.625	22.1	36.85
17	25.95	21.6	36.125	16.325
18	24.625	18.775	33.7	22.900000000000002
19	36.175000000000004	14.75	31.05	18.025
20	42.025	16.875	25.8	15.299999999999999
21	23.9	20.599999999999998	24.15	31.35
22	28.325	14.85	44.074999999999996	12.75
23	27.075	14.475	41.55	16.900000000000002
24	48.4	16.8	23.7	11.1
25	47.5	23.875	15.5	13.125
26	27.425	43.65	15.9	13.025
27	17.275	44.625	24.275	13.825000000000001
28	13.3	25.575	45.35	15.775
29	12.0	14.174999999999999	53.474999999999994	20.349999999999998
30	11.4	10.525	34.300000000000004	43.775
31	15.6	7.95	44.125	32.324999999999996
32	28.175	8.9	21.65	41.275
33	51.025	11.425	15.4	22.15
34	57.15	9.85	17.1	15.9
35	59.150000000000006	9.175	19.025	12.65
36	43.2	10.75	30.85	15.2
37	42.3	12.45	21.0	24.25
38	23.625	16.775000000000002	13.475000000000001	46.125
39	17.1	24.4	11.675	46.825
40	17.0	44.5	14.674999999999999	23.825
41	26.35	45.375	11.125	17.150000000000002
42	48.0	28.1	13.475000000000001	10.424999999999999
43	47.575	30.65	9.925	11.85
44	28.975	47.199999999999996	9.375	14.45
45	18.9	48.475	14.6	18.025
46	14.424999999999999	26.650000000000002	21.325	37.6
47	11.325000000000001	16.925	37.675	34.075
48	12.975	14.35	20.424999999999997	52.25
49	15.85	20.225	15.575	48.35
50	20.125	35.35	15.024999999999999	29.5
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	2.0
23	1.0
24	0.0
25	1.0
26	2.0
27	3.0
28	4.0
29	3.5
30	3.0
31	20.5
32	38.0
33	32.0
34	26.0
35	42.5
36	59.0
37	66.0
38	73.0
39	75.5
40	78.0
41	79.0
42	80.0
43	97.5
44	115.0
45	162.0
46	209.0
47	298.5
48	388.0
49	486.0
50	584.0
51	680.5
52	777.0
53	591.0
54	405.0
55	436.5
56	468.0
57	382.5
58	297.0
59	188.5
60	80.0
61	64.5
62	49.0
63	87.5
64	126.0
65	79.5
66	33.0
67	22.5
68	12.0
69	18.0
70	24.0
71	33.5
72	43.0
73	27.0
74	11.0
75	6.0
76	1.0
77	2.5
78	4.0
79	4.5
80	5.0
81	3.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27499999999999997
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.55
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8564867967853	32.6
2	11.710677382319174	10.2
3	3.5017221584385765	4.575
4	2.4110218140068884	4.2
5	1.1481056257175661	2.5
6	1.2055109070034442	3.15
7	0.4592422502870264	1.4000000000000001
8	0.9184845005740528	3.2
9	0.5166475315729048	2.025
>10	2.755453501722158	19.025
>50	0.4592422502870264	14.124999999999998
>100	0.0574052812858783	3.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	120	3.0	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	97	2.4250000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	83	2.075	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	77	1.925	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	54	1.35	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	54	1.35	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	22	0.5499999999999999	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	20	0.5	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	17	0.42500000000000004	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG	17	0.42500000000000004	RNA PCR Primer, Index 2 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC	16	0.4	RNA PCR Primer, Index 4 (100% over 44bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	16	0.4	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	15	0.375	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	13	0.325	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	12	0.3	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	12	0.3	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	11	0.27499999999999997	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	11	0.27499999999999997	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	10	0.25	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	10	0.25	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	10	0.25	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 4 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	9	0.22499999999999998	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	8	0.2	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	8	0.2	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	8	0.2	No Hit
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	8	0.2	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	7	0.17500000000000002	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	6	0.15	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
TCTCACACAGAAATCGCACCCGTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	5	0.125	No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	5	0.125	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	5	0.125	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.3	0.0	0.0	0.0
3	0.0	0.375	0.0	0.0	0.0
4	0.0	0.45	0.0	0.0	0.0
5	0.0	0.5	0.0	0.0	0.0
6	0.0	0.6	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.375	0.0	0.0	0.0
9	0.0	1.5	0.0	0.0	0.0
10	0.0	1.55	0.0	0.0	0.0
11	0.0	1.725	0.0	0.0	0.0
12	0.0	2.05	0.0	0.0	0.0
13	0.0	2.325	0.0	0.0	0.0
14	0.0	2.5	0.0	0.0	0.0
15	0.0	2.925	0.0	0.0	0.0
16	0.0	3.825	0.0	0.0	0.0
17	0.0	4.4	0.0	0.0	0.0
18	0.0	5.325	0.0	0.0	0.0
19	0.0	5.675	0.0	0.0	0.0
20	0.0	6.675	0.0	0.0	0.0
21	0.0	11.5	0.0	0.0	0.0
22	0.0	21.875	0.0	0.0	0.0
23	0.0	47.95	0.0	0.0	0.0
24	0.0	59.025	0.0	0.0	0.0
25	0.0	64.025	0.0	0.0	0.0
26	0.0	65.5	0.0	0.0	0.0
27	0.0	66.3	0.0	0.0	0.0
28	0.0	66.675	0.0	0.0	0.0
29	0.0	67.325	0.0	0.0	0.0
30	0.0	67.675	0.0	0.0	0.0
31	0.0	68.525	0.0	0.0	0.0
32	0.0	69.525	0.0	0.0	0.0
33	0.0	70.45	0.0	0.0	0.0
34	0.0	73.075	0.0	0.0	0.0
35	0.0	73.95	0.0	0.0	0.0
36	0.0	74.65	0.0	0.0	0.0
37	0.0	75.625	0.0	0.0	0.0
38	0.0	76.15	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
TGTTGGA	20	6.952051E-4	44.000004	20
CTGAGAC	20	6.952051E-4	44.000004	4
CCTGAGA	20	6.952051E-4	44.000004	3
GGCTGGA	20	6.952051E-4	44.000004	19
CATTCAA	20	6.952051E-4	44.000004	3
ACATTCA	20	6.952051E-4	44.000004	2
CCCTGAG	20	6.952051E-4	44.000004	2
TTGGCTG	20	6.952051E-4	44.000004	17
AGACCCT	20	6.952051E-4	44.000004	7
ATTCAAC	20	6.952051E-4	44.000004	4
GAGACCC	20	6.952051E-4	44.000004	6
TGGCTGG	20	6.952051E-4	44.000004	18
TCCCTGA	20	6.952051E-4	44.000004	1
ATGATGA	25	3.6958358E-5	44.0	5
GCTTGGC	50	1.4551915E-11	44.0	15
GAGCTTG	60	1.8189894E-12	40.333332	13
GGATCCG	60	1.8189894E-12	40.333332	3
CCGTCTG	60	1.8189894E-12	40.333332	7
TCGGATC	60	1.8189894E-12	40.333332	1
>>END_MODULE
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
Read 239724 spots for SRR5030954.sra
Written 239724 spots for SRR5030954.sra
Read 239715 spots for SRR5030954.sra
Written 239715 spots for SRR5030954.sra
SRR ids: ['SRR5030954.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_nxmjzvf1
SRR5030954.sra spots: 2876589
blocks: [[1, 239715], [239716, 479430], [479431, 719145], [719146, 958860], [958861, 1198575], [1198576, 1438290], [1438291, 1678005], [1678006, 1917720], [1917721, 2157435], [2157436, 2397150], [2397151, 2636865], [2636866, 2876589]]
SRR5030954 file size 497499
SRR5030954 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030954 SRR5030954_1.fastq
Input file:	SRR5030954_1.fastq
trimmed:	SRR5030954-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:52:58 2020 >> started

Wed Mar  4 06:53:01 2020 >> done (2.988s)
2876589 reads processed; of these:
    184 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2876399 (99.99%) reads available; of these:
 221060 ( 7.69%) trimmed reads available after processing
2655339 (92.31%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     56	  0.00%
 19	     62	  0.00%
 20	    164	  0.01%
 21	    197	  0.01%
 22	    311	  0.01%
 23	    359	  0.01%
 24	    386	  0.01%
 25	    443	  0.02%
 26	    447	  0.02%
 27	    624	  0.02%
 28	    667	  0.02%
 29	   1044	  0.04%
 30	    752	  0.03%
 31	   1045	  0.04%
 32	   1457	  0.05%
 33	   2375	  0.08%
 34	   7320	  0.25%
 35	   3519	  0.12%
 36	   2528	  0.09%
 37	   2890	  0.10%
 38	   3922	  0.14%
 39	   3837	  0.13%
 40	   5877	  0.20%
 41	   4351	  0.15%
 42	   7299	  0.25%
 43	   6163	  0.21%
 44	  16448	  0.57%
 45	  15236	  0.53%
 46	  14756	  0.51%
 47	  18504	  0.64%
 48	  39750	  1.38%
 49	  58271	  2.03%
 50	2655339	 92.31%
2876399 reads passed initial QC


criterion=sequence-density
sequence-density=74.33
sequence-density-rank=1
fanout-score=20.00
fanout-score-rank=2
prefix-density=77.16
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC


criterion=fanout-score
sequence-density=2.01
sequence-density-rank=7
fanout-score=117.08
fanout-score-rank=1
prefix-density=76.13
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC -o SRR5030954 -
Input file:	STDIN
trimmed:	SRR5030954-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:53:18 2020 >> started

Wed Mar  4 06:53:25 2020 >> done (7.311s)
2799695 reads processed; of these:
 159534 ( 5.70%) short reads filtered out after trimming by size control
   8661 ( 0.31%) empty reads filtered out after trimming by size control
2631500 (93.99%) reads available; of these:
2244117 (85.28%) trimmed reads available after processing
 387383 (14.72%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  14294	  0.54%
 19	  21027	  0.80%
 20	 135580	  5.15%
 21	 268028	 10.19%
 22	 720086	 27.36%
 23	 297668	 11.31%
 24	 150508	  5.72%
 25	  42638	  1.62%
 26	  21022	  0.80%
 27	  16676	  0.63%
 28	  18022	  0.68%
 29	  14366	  0.55%
 30	  20556	  0.78%
 31	  31977	  1.22%
 32	  28681	  1.09%
 33	  68825	  2.62%
 34	  29888	  1.14%
 35	  23520	  0.89%
 36	  25776	  0.98%
 37	  18245	  0.69%
 38	  19278	  0.73%
 39	  21153	  0.80%
 40	  31630	  1.20%
 41	  38326	  1.46%
 42	  46018	  1.75%
 43	  33451	  1.27%
 44	  28049	  1.07%
 45	  36328	  1.38%
 46	  29191	  1.11%
 47	  22905	  0.87%
 48	   4460	  0.17%
 49	  13847	  0.53%
 50	 339481	 12.90%


criterion=sequence-density
sequence-density=5.48
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.70
sequence-density-rank=18
fanout-score=12.04
fanout-score-rank=1
prefix-density=8.41
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:53:57
                             Started mapping on |	Mar 04 06:53:58
                                    Finished on |	Mar 04 06:54:19
       Mapping speed, Million of reads per hour |	464.26

                          Number of input reads |	2708204
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1624457
                        Uniquely mapped reads % |	59.98%
                          Average mapped length |	28.78
                       Number of splices: Total |	1133
            Number of splices: Annotated (sjdb) |	255
                       Number of splices: GT/AG |	981
                       Number of splices: GC/AG |	22
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	129
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.43
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	723381
             % of reads mapped to multiple loci |	26.71%
        Number of reads mapped to too many loci |	73549
             % of reads mapped to too many loci |	2.72%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.50%
                     % of reads unmapped: other |	1.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	360366	360366	360366
N_multimapping	723381	723381	723381
N_noFeature	83340	106142	1145486
N_ambiguous	863406	445100	19348
UnstrandedReadsAssigned:677711 PositiveStrandReadsAssigned:1073215 NegativeStrandReadsAssigned:459623
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030954 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030954-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,708,204 reads, 714,303 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 782 rounds

 109283 SRR5030954.ke.tsv
  52636 SRR5030954.se.tsv
 161919 total
==> SRR5030954.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030954.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030954 completed mapping pipeline successfully
