Starting /dee2/code/volunteer_pipeline.sh SRR5031059
    current disk space = 160510033920
    free memory = 73912332 
SRR5031059 SRAfilesize
6d510d24836ab360c8e6b70b46fadb36  SRR5031059.sra
SRR5031059.sra file validated
SRR5031059 is single end
SRR5031059 is conventional basespace
SRR5031059 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35425	33.0	31.0	34.0	31.0	34.0
2	32.65175	34.0	31.0	34.0	31.0	34.0
3	32.62475	34.0	31.0	34.0	31.0	34.0
4	36.1985	37.0	37.0	37.0	35.0	37.0
5	35.9275	37.0	35.0	37.0	35.0	37.0
6	35.86725	37.0	35.0	37.0	35.0	37.0
7	35.96925	37.0	35.0	37.0	35.0	37.0
8	35.81325	37.0	35.0	37.0	35.0	37.0
9	37.60075	39.0	37.0	39.0	35.0	39.0
10	37.7545	39.0	38.0	39.0	35.0	39.0
11	37.5725	39.0	37.0	39.0	35.0	39.0
12	37.615	39.0	37.0	39.0	35.0	39.0
13	37.64625	39.0	37.0	39.0	35.0	39.0
14	38.9835	40.0	38.0	41.0	35.0	41.0
15	38.95875	40.0	38.0	41.0	35.0	41.0
16	38.913	40.0	38.0	41.0	35.0	41.0
17	38.816	40.0	38.0	41.0	35.0	41.0
18	38.92225	40.0	38.0	41.0	35.0	41.0
19	38.6425	40.0	38.0	41.0	34.0	41.0
20	38.7715	40.0	38.0	41.0	34.0	41.0
21	38.77475	40.0	38.0	41.0	34.0	41.0
22	38.90775	40.0	38.0	41.0	35.0	41.0
23	38.80325	40.0	38.0	41.0	35.0	41.0
24	38.805	40.0	38.0	41.0	35.0	41.0
25	38.912	40.0	39.0	41.0	35.0	41.0
26	38.75275	40.0	38.0	41.0	34.0	41.0
27	38.68725	40.0	38.0	41.0	34.0	41.0
28	38.6525	40.0	38.0	41.0	34.0	41.0
29	38.06175	40.0	38.0	41.0	33.0	41.0
30	38.09075	40.0	38.0	41.0	33.0	41.0
31	38.30425	40.0	38.0	41.0	34.0	41.0
32	38.2535	40.0	38.0	41.0	33.0	41.0
33	38.1835	40.0	38.0	41.0	33.0	41.0
34	38.39025	40.0	38.0	41.0	34.0	41.0
35	38.26575	40.0	38.0	41.0	34.0	41.0
36	37.81775	40.0	37.0	41.0	33.0	41.0
37	37.31575	40.0	37.0	41.0	31.0	41.0
38	36.774	39.0	36.0	41.0	30.0	41.0
39	37.31275	39.0	37.0	41.0	31.0	41.0
40	37.57525	40.0	37.0	41.0	33.0	41.0
41	37.601	40.0	37.0	41.0	32.0	41.0
42	37.89825	40.0	37.0	41.0	33.0	41.0
43	38.0805	40.0	38.0	41.0	33.0	41.0
44	37.80775	40.0	37.0	41.0	33.0	41.0
45	37.68875	40.0	37.0	41.0	33.0	41.0
46	37.65375	40.0	37.0	41.0	33.0	41.0
47	37.1525	40.0	36.0	41.0	31.0	41.0
48	36.80925	40.0	36.0	41.0	31.0	41.0
49	36.8895	40.0	36.0	41.0	31.0	41.0
50	36.31225	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	2.0
18	1.0
19	1.0
20	1.0
21	6.0
22	3.0
23	10.0
24	10.0
25	9.0
26	19.0
27	22.0
28	26.0
29	35.0
30	54.0
31	72.0
32	87.0
33	108.0
34	176.0
35	155.0
36	240.0
37	444.0
38	703.0
39	1813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.5	24.05	49.675000000000004	14.774999999999999
2	24.674674674674673	26.95195195195195	18.81881881881882	29.554554554554553
3	23.925	19.775000000000002	20.9	35.4
4	35.025	27.875	14.725	22.375
5	24.325	36.325	25.974999999999998	13.375
6	31.075000000000003	14.85	40.575	13.5
7	18.325	22.525000000000002	20.8	38.35
8	23.05	29.525000000000002	16.3	31.125000000000004
9	23.175	32.5	27.975	16.35
10	21.675	21.275	29.775000000000002	27.275
11	33.050000000000004	13.100000000000001	15.55	38.3
12	15.35	20.424999999999997	31.55	32.675
13	28.349999999999998	18.125	33.425	20.1
14	29.9	28.749999999999996	23.549999999999997	17.8
15	37.775	21.55	26.025	14.649999999999999
16	17.075000000000003	22.0	23.025000000000002	37.9
17	27.025	23.625	35.225	14.124999999999998
18	25.224999999999998	15.975	33.074999999999996	25.724999999999998
19	35.425000000000004	15.7	30.575000000000003	18.3
20	45.95	14.875	25.324999999999996	13.850000000000001
21	24.05	21.099999999999998	25.374999999999996	29.475
22	29.15	14.899999999999999	45.775	10.174999999999999
23	24.25	13.875000000000002	45.775	16.1
24	50.2	15.325	25.650000000000002	8.825
25	51.0	22.675	15.55	10.775
26	27.900000000000002	46.800000000000004	12.9	12.4
27	16.6	47.349999999999994	22.8	13.25
28	11.95	24.8	47.825	15.425
29	11.825	12.125	56.625	19.425
30	10.674999999999999	6.6000000000000005	35.325	47.4
31	14.149999999999999	5.75	46.35	33.75
32	26.125	6.3	23.525	44.05
33	53.725	8.975	14.774999999999999	22.525000000000002
34	61.7	7.35	15.950000000000001	15.0
35	64.64999999999999	7.9	17.625	9.825000000000001
36	39.75	8.475000000000001	37.775	14.000000000000002
37	45.1	11.375	21.625	21.9
38	24.425	16.725	12.8	46.050000000000004
39	15.875	21.875	10.775	51.475
40	16.025	46.675	12.2	25.1
41	22.325	49.75	10.525	17.4
42	48.575	28.575	12.8	10.05
43	53.77499999999999	25.825	8.575000000000001	11.825
44	31.775	47.075	7.775	13.375
45	18.375	49.95	14.000000000000002	17.675
46	14.899999999999999	26.150000000000002	16.8	42.15
47	10.65	14.399999999999999	39.4	35.55
48	12.775	12.125	20.175	54.925000000000004
49	13.0	18.224999999999998	13.975000000000001	54.800000000000004
50	17.025000000000002	39.275	12.049999999999999	31.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	0.5
26	0.0
27	0.0
28	0.0
29	2.0
30	4.0
31	8.5
32	13.0
33	13.0
34	13.0
35	19.0
36	25.0
37	35.5
38	46.0
39	45.0
40	44.0
41	57.0
42	70.0
43	97.0
44	124.0
45	144.0
46	164.0
47	286.5
48	409.0
49	499.5
50	590.0
51	726.5
52	863.0
53	640.0
54	417.0
55	475.5
56	534.0
57	404.5
58	275.0
59	181.0
60	87.0
61	68.5
62	50.0
63	102.0
64	154.0
65	92.5
66	31.0
67	20.5
68	10.0
69	12.5
70	15.0
71	32.0
72	49.0
73	27.0
74	5.0
75	3.0
76	1.0
77	0.5
78	0.0
79	2.0
80	4.0
81	2.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83497053045186	28.95
2	9.561231172233137	7.3
3	3.798297314996726	4.35
4	2.4885396201702688	3.8
5	1.309757694826457	2.5
6	0.8513425016371972	1.95
7	1.0478061558611658	2.8000000000000003
8	0.9823182711198428	3.0
9	0.5239030779305829	1.7999999999999998
>10	2.9469548133595285	19.425
>50	0.3274394237066143	8.475000000000001
>100	0.3274394237066143	15.65
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	149	3.7249999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	139	3.4750000000000005	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	133	3.325	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	102	2.55	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	87	2.175	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	72	1.7999999999999998	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	65	1.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	57	1.425	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	43	1.075	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	40	1.0	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	39	0.975	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC	23	0.575	RNA PCR Primer, Index 31 (100% over 44bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	21	0.525	RNA PCR Primer, Index 7 (100% over 35bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	18	0.44999999999999996	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	16	0.4	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 31 (100% over 50bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	12	0.3	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	10	0.25	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	9	0.22499999999999998	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	9	0.22499999999999998	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	8	0.2	No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	8	0.2	RNA PCR Primer, Index 2 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	7	0.17500000000000002	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGAATTGTGGCTGGACATCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATGCTCAATAGGATTACGCTGAGGCCCTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGGCAGTGTAGTTAGCTGATTGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTG	5	0.125	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGTG	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.45	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.525	0.0	0.0	0.0
6	0.0	0.575	0.0	0.0	0.0
7	0.0	1.25	0.0	0.0	0.0
8	0.0	1.325	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.575	0.0	0.0	0.0
11	0.0	1.725	0.0	0.0	0.0
12	0.0	2.0	0.0	0.0	0.0
13	0.0	2.175	0.0	0.0	0.0
14	0.0	2.45	0.0	0.0	0.0
15	0.0	2.775	0.0	0.0	0.0
16	0.0	3.775	0.0	0.0	0.0
17	0.0	4.475	0.0	0.0	0.0
18	0.0	5.2	0.0	0.0	0.0
19	0.0	5.6	0.0	0.0	0.0
20	0.0	6.525	0.0	0.0	0.0
21	0.0	11.4	0.0	0.0	0.0
22	0.0	20.975	0.0	0.0	0.0
23	0.0	50.625	0.0	0.0	0.0
24	0.0	64.0	0.0	0.0	0.0
25	0.0	69.6	0.0	0.0	0.0
26	0.0	70.875	0.0	0.0	0.0
27	0.0	71.7	0.0	0.0	0.0
28	0.0	72.625	0.0	0.0	0.0
29	0.0	73.45	0.0	0.0	0.0
30	0.0	74.0	0.0	0.0	0.0
31	0.0	74.75	0.0	0.0	0.0
32	0.0	76.875	0.0	0.0	0.0
33	0.0	78.15	0.0	0.0	0.0
34	0.0	81.7	0.0	0.0	0.0
35	0.0	82.975	0.0	0.0	0.0
36	0.0	84.65	0.0	0.0	0.0
37	0.0	85.925	0.0	0.0	0.0
38	0.0	86.725	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
CTGAGAC	20	6.952051E-4	44.000004	4
ATCCAGG	20	6.952051E-4	44.000004	9
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
ATAGGCT	20	6.952051E-4	44.000004	16
GAGCTTG	40	5.315087E-9	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
TCGGATC	40	5.315087E-9	44.000004	1
CGGATCC	40	5.315087E-9	44.000004	2
TCCAGGA	20	6.952051E-4	44.000004	10
GTGAGTG	20	6.952051E-4	44.000004	18
ACATTCA	40	5.315087E-9	44.000004	2
GGATAGG	20	6.952051E-4	44.000004	14
AGTAATC	20	6.952051E-4	44.000004	5
GATCCGT	40	5.315087E-9	44.000004	4
TAATCCA	20	6.952051E-4	44.000004	7
>>END_MODULE
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198475 spots for SRR5031059.sra
Written 198475 spots for SRR5031059.sra
Read 198485 spots for SRR5031059.sra
Written 198485 spots for SRR5031059.sra
SRR ids: ['SRR5031059.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5uj3ia3q
SRR5031059.sra spots: 2381710
blocks: [[1, 198475], [198476, 396950], [396951, 595425], [595426, 793900], [793901, 992375], [992376, 1190850], [1190851, 1389325], [1389326, 1587800], [1587801, 1786275], [1786276, 1984750], [1984751, 2183225], [2183226, 2381710]]
SRR5031059 file size 411734
SRR5031059 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031059 SRR5031059_1.fastq
Input file:	SRR5031059_1.fastq
trimmed:	SRR5031059-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:15:36 2020 >> started

Wed Mar  4 07:15:39 2020 >> done (2.904s)
2381710 reads processed; of these:
    309 ( 0.01%) short reads filtered out after trimming by size control
     17 ( 0.00%) empty reads filtered out after trimming by size control
2381384 (99.99%) reads available; of these:
 124099 ( 5.21%) trimmed reads available after processing
2257285 (94.79%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     78	  0.00%
 19	     85	  0.00%
 20	    158	  0.01%
 21	    227	  0.01%
 22	    344	  0.01%
 23	    402	  0.02%
 24	    348	  0.01%
 25	    522	  0.02%
 26	    380	  0.02%
 27	    556	  0.02%
 28	    667	  0.03%
 29	   1000	  0.04%
 30	    565	  0.02%
 31	    696	  0.03%
 32	    795	  0.03%
 33	    928	  0.04%
 34	   1232	  0.05%
 35	   2372	  0.10%
 36	   2004	  0.08%
 37	   2370	  0.10%
 38	   2287	  0.10%
 39	   2297	  0.10%
 40	   2700	  0.11%
 41	   2732	  0.11%
 42	   3476	  0.15%
 43	   5100	  0.21%
 44	   6641	  0.28%
 45	   7821	  0.33%
 46	  11825	  0.50%
 47	  14981	  0.63%
 48	  23818	  1.00%
 49	  24692	  1.04%
 50	2257285	 94.79%
2381384 reads passed initial QC


criterion=sequence-density
sequence-density=86.69
sequence-density-rank=1
fanout-score=22.27
fanout-score-rank=1
prefix-density=88.89
prefix-fanout=21.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC


criterion=fanout-score
sequence-density=86.69
sequence-density-rank=1
fanout-score=22.27
fanout-score-rank=1
prefix-density=88.89
prefix-fanout=21.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC -o SRR5031059 -
Input file:	STDIN
trimmed:	SRR5031059-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:15:58 2020 >> started

Wed Mar  4 07:16:04 2020 >> done (5.740s)
2326640 reads processed; of these:
 123957 ( 5.33%) short reads filtered out after trimming by size control
  11251 ( 0.48%) empty reads filtered out after trimming by size control
2191432 (94.19%) reads available; of these:
2092419 (95.48%) trimmed reads available after processing
  99013 ( 4.52%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  11981	  0.55%
 19	  21059	  0.96%
 20	 111064	  5.07%
 21	 246418	 11.24%
 22	 679302	 31.00%
 23	 320641	 14.63%
 24	 143619	  6.55%
 25	  40936	  1.87%
 26	  20927	  0.95%
 27	  17303	  0.79%
 28	  18892	  0.86%
 29	  15701	  0.72%
 30	  21865	  1.00%
 31	  46260	  2.11%
 32	  28785	  1.31%
 33	  78076	  3.56%
 34	  32364	  1.48%
 35	  28059	  1.28%
 36	  33841	  1.54%
 37	  13560	  0.62%
 38	  16175	  0.74%
 39	  13461	  0.61%
 40	  20501	  0.94%
 41	  23834	  1.09%
 42	  27380	  1.25%
 43	  19036	  0.87%
 44	  13470	  0.61%
 45	  18001	  0.82%
 46	  12876	  0.59%
 47	  10262	  0.47%
 48	    978	  0.04%
 49	   1375	  0.06%
 50	  83430	  3.81%


criterion=sequence-density
sequence-density=8.66
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.48
sequence-density-rank=32
fanout-score=16.15
fanout-score-rank=1
prefix-density=7.77
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 07:16:36
                             Started mapping on |	Mar 04 07:16:37
                                    Finished on |	Mar 04 07:17:01
       Mapping speed, Million of reads per hour |	336.93

                          Number of input reads |	2246176
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1184820
                        Uniquely mapped reads % |	52.75%
                          Average mapped length |	24.76
                       Number of splices: Total |	1610
            Number of splices: Annotated (sjdb) |	316
                       Number of splices: GT/AG |	1504
                       Number of splices: GC/AG |	30
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	76
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.76
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	773593
             % of reads mapped to multiple loci |	34.44%
        Number of reads mapped to too many loci |	51863
             % of reads mapped to too many loci |	2.31%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.59%
                     % of reads unmapped: other |	0.91%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	287763	287763	287763
N_multimapping	773593	773593	773593
N_noFeature	80952	95917	814276
N_ambiguous	627616	301961	9515
UnstrandedReadsAssigned:476252 PositiveStrandReadsAssigned:786942 NegativeStrandReadsAssigned:361029
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031059 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031059-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,246,176 reads, 535,678 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 722 rounds

 109283 SRR5031059.ke.tsv
  52636 SRR5031059.se.tsv
 161919 total
==> SRR5031059.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031059.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031059 completed mapping pipeline successfully
