Starting /dee2/code/volunteer_pipeline.sh SRR5030975
    current disk space = 160587710464
    free memory = 73933576 
SRR5030975 SRAfilesize
473c3dd3b73bf220da02fc8373161b72  SRR5030975.sra
SRR5030975.sra file validated
SRR5030975 is single end
SRR5030975 is conventional basespace
SRR5030975 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.503	34.0	31.0	34.0	31.0	34.0
2	32.80475	34.0	31.0	34.0	31.0	34.0
3	32.8135	34.0	31.0	34.0	31.0	34.0
4	36.31875	37.0	37.0	37.0	35.0	37.0
5	36.17325	37.0	37.0	37.0	35.0	37.0
6	35.9745	37.0	35.0	37.0	35.0	37.0
7	36.05875	37.0	35.0	37.0	35.0	37.0
8	36.095	37.0	36.0	37.0	35.0	37.0
9	37.774	39.0	38.0	39.0	35.0	39.0
10	37.921	39.0	38.0	39.0	35.0	39.0
11	37.77275	39.0	38.0	39.0	35.0	39.0
12	37.78475	39.0	38.0	39.0	35.0	39.0
13	37.794	39.0	38.0	39.0	35.0	39.0
14	39.0185	41.0	38.0	41.0	35.0	41.0
15	39.093	41.0	39.0	41.0	36.0	41.0
16	39.24525	41.0	39.0	41.0	36.0	41.0
17	39.32725	41.0	39.0	41.0	36.0	41.0
18	39.33775	41.0	39.0	41.0	36.0	41.0
19	39.049	40.0	39.0	41.0	35.0	41.0
20	39.0635	40.0	39.0	41.0	35.0	41.0
21	39.09175	40.0	39.0	41.0	36.0	41.0
22	39.01125	40.0	39.0	41.0	35.0	41.0
23	38.8775	40.0	39.0	41.0	35.0	41.0
24	38.992	40.0	39.0	41.0	35.0	41.0
25	39.05975	40.0	39.0	41.0	35.0	41.0
26	38.67575	40.0	38.0	41.0	34.0	41.0
27	38.84875	40.0	38.0	41.0	35.0	41.0
28	38.3255	40.0	38.0	41.0	34.0	41.0
29	37.816	40.0	38.0	41.0	32.0	41.0
30	38.06725	40.0	38.0	41.0	33.0	41.0
31	38.324	40.0	38.0	41.0	33.0	41.0
32	38.2105	40.0	38.0	41.0	34.0	41.0
33	38.29825	40.0	38.0	41.0	34.0	41.0
34	38.2185	40.0	38.0	41.0	34.0	41.0
35	37.98675	40.0	38.0	41.0	33.0	41.0
36	37.39	40.0	37.0	41.0	31.0	41.0
37	37.2165	39.0	37.0	41.0	31.0	41.0
38	37.425	39.0	37.0	41.0	32.0	41.0
39	37.68675	40.0	37.0	41.0	33.0	41.0
40	37.6745	40.0	37.0	41.0	33.0	41.0
41	37.71175	40.0	37.0	41.0	33.0	41.0
42	37.75825	40.0	37.0	41.0	33.0	41.0
43	37.87875	40.0	37.0	41.0	33.0	41.0
44	37.62275	40.0	37.0	41.0	33.0	41.0
45	37.484	40.0	37.0	41.0	33.0	41.0
46	36.829	40.0	36.0	41.0	31.0	41.0
47	36.39475	39.0	35.0	41.0	30.0	41.0
48	35.7375	39.0	34.0	41.0	28.0	41.0
49	36.14475	39.0	35.0	41.0	30.0	41.0
50	35.49625	39.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	1.0
19	2.0
20	2.0
21	1.0
22	7.0
23	9.0
24	8.0
25	8.0
26	17.0
27	20.0
28	31.0
29	34.0
30	48.0
31	62.0
32	80.0
33	101.0
34	160.0
35	189.0
36	235.0
37	479.0
38	862.0
39	1640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.482111583687766	25.068801601200903	49.337002752064045	16.112084063047284
2	22.1	27.35	19.375	31.175000000000004
3	24.9	19.3	23.974999999999998	31.825
4	34.1	27.450000000000003	15.225	23.225
5	22.355588897224308	37.53438359589897	28.157039259814955	11.952988247061766
6	33.324999999999996	15.925	35.425000000000004	15.325
7	16.0	21.825	22.2	39.975
8	24.4	27.825	16.475	31.3
9	24.275	31.424999999999997	27.875	16.425
10	21.975	20.95	28.9	28.175
11	30.8	10.2	18.85	40.150000000000006
12	16.6	16.275000000000002	32.775	34.35
13	32.1	16.1	32.85	18.95
14	30.625000000000004	32.525	19.175	17.675
15	36.675000000000004	22.7	26.424999999999997	14.2
16	18.35	18.3	24.45	38.9
17	28.449999999999996	19.175	38.375	14.000000000000002
18	28.225	16.725	29.375	25.674999999999997
19	37.125	13.900000000000002	32.125	16.85
20	45.6	15.75	25.124999999999996	13.525
21	21.925	21.9	27.250000000000004	28.925
22	30.2	14.2	45.1	10.5
23	28.175	13.25	43.225	15.35
24	49.25	15.125	28.050000000000004	7.575
25	49.475	23.9	16.0	10.625
26	30.599999999999998	43.85	15.425	10.125
27	16.85	45.725	24.65	12.775
28	13.3	24.6	46.85	15.25
29	12.3	12.925	55.974999999999994	18.8
30	11.899999999999999	6.0249999999999995	36.675000000000004	45.4
31	15.85	4.65	44.425	35.075
32	28.225	5.949999999999999	22.25	43.575
33	52.5	9.4	14.025000000000002	24.075
34	60.02499999999999	7.6	17.275	15.1
35	65.05	7.5	18.7	8.75
36	42.95	7.7	35.099999999999994	14.249999999999998
37	43.15	10.925	21.275	24.65
38	24.575	17.9	12.25	45.275
39	16.150000000000002	24.375	10.45	49.025
40	15.299999999999999	46.375	11.725	26.6
41	25.3	47.825	9.1	17.775
42	45.525	31.525	11.85	11.1
43	50.8	29.7	7.9750000000000005	11.525
44	31.75	45.025	8.15	15.075
45	18.625	48.5	13.675	19.2
46	13.675	26.974999999999998	17.45	41.9
47	8.7	15.675	37.875	37.75
48	10.674999999999999	12.85	21.9	54.574999999999996
49	13.075000000000001	19.35	16.2	51.37500000000001
50	17.724999999999998	37.4	13.675	31.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	3.0
30	4.0
31	9.0
32	14.0
33	13.5
34	13.0
35	11.0
36	9.0
37	16.0
38	23.0
39	28.5
40	34.0
41	44.5
42	55.0
43	77.0
44	99.0
45	150.0
46	201.0
47	315.0
48	429.0
49	525.5
50	622.0
51	735.0
52	848.0
53	671.5
54	495.0
55	512.5
56	530.0
57	390.5
58	251.0
59	162.5
60	74.0
61	60.5
62	47.0
63	64.0
64	81.0
65	57.5
66	34.0
67	25.0
68	16.0
69	20.0
70	24.0
71	54.5
72	85.0
73	46.0
74	7.0
75	4.0
76	1.0
77	0.5
78	0.0
79	0.5
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.025
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73194534808067	29.099999999999998
2	9.694209499024073	7.449999999999999
3	4.554326610279766	5.25
4	1.8217306441119063	2.8000000000000003
5	1.626545217957059	3.125
6	1.3012361743656473	3.0
7	1.1711125569290826	3.15
8	0.6506180871828237	2.0
9	0.32530904359141183	1.125
>10	2.5374105400130125	21.25
>50	0.1951854261548471	4.075
>100	0.3903708523096942	17.675
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	123	3.075	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	121	3.025	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	120	3.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	105	2.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	56	1.4000000000000001	RNA PCR Primer, Index 7 (97% over 35bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	49	1.225	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	48	1.2	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	48	1.2	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	42	1.05	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC	19	0.475	RNA PCR Primer, Index 42 (100% over 44bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	15	0.375	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	15	0.375	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	13	0.325	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	12	0.3	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	8	0.2	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	8	0.2	RNA PCR Primer, Index 3 (100% over 34bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC	8	0.2	RNA PCR Primer, Index 42 (100% over 50bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	6	0.15	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCG	6	0.15	RNA PCR Primer, Index 42 (100% over 45bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	6	0.15	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA	6	0.15	RNA PCR Primer, Index 3 (97% over 36bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGCACTCCAGTCACTA	5	0.125	RNA PCR Primer, Index 10 (97% over 35bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.275	0.0	0.0	0.0
2	0.0	0.325	0.0	0.0	0.0
3	0.0	0.475	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.6	0.0	0.0	0.0
6	0.0	0.8	0.0	0.0	0.0
7	0.0	1.4	0.0	0.0	0.0
8	0.0	1.6	0.0	0.0	0.0
9	0.0	1.65	0.0	0.0	0.0
10	0.0	1.825	0.0	0.0	0.0
11	0.0	2.25	0.0	0.0	0.0
12	0.0	2.4	0.0	0.0	0.0
13	0.0	2.65	0.0	0.0	0.0
14	0.0	3.025	0.0	0.0	0.0
15	0.0	3.7	0.0	0.0	0.0
16	0.0	5.8	0.0	0.0	0.0
17	0.0	6.575	0.0	0.0	0.0
18	0.0	8.175	0.0	0.0	0.0
19	0.0	8.75	0.0	0.0	0.0
20	0.0	9.775	0.0	0.0	0.0
21	0.0	15.9	0.0	0.0	0.0
22	0.0	27.025	0.0	0.0	0.0
23	0.0	54.7	0.0	0.0	0.0
24	0.0	68.575	0.0	0.0	0.0
25	0.0	74.575	0.0	0.0	0.0
26	0.0	76.125	0.0	0.0	0.0
27	0.0	76.825	0.0	0.0	0.0
28	0.0	77.475	0.0	0.0	0.0
29	0.0	78.35	0.0	0.0	0.0
30	0.0	78.775	0.0	0.0	0.0
31	0.0	79.775	0.0	0.0	0.0
32	0.0	80.75	0.0	0.0	0.0
33	0.0	81.775	0.0	0.0	0.0
34	0.0	84.65	0.0	0.0	0.0
35	0.0	85.85	0.0	0.0	0.0
36	0.0	87.05	0.0	0.0	0.0
37	0.0	88.0	0.0	0.0	0.0
38	0.0	88.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.952051E-4	44.000004	2
GTATGAT	20	6.952051E-4	44.000004	18
TCTAGCT	20	6.952051E-4	44.000004	11
ATCTAGC	20	6.952051E-4	44.000004	10
TTATCTA	20	6.952051E-4	44.000004	8
TAGCTGT	20	6.952051E-4	44.000004	13
TATGATG	20	6.952051E-4	44.000004	19
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGTAT	20	6.952051E-4	44.000004	15
TATCTAG	20	6.952051E-4	44.000004	9
GAACCAT	20	6.952051E-4	44.000004	4
GGTAGTA	20	6.952051E-4	44.000004	4
CTAGCTG	20	6.952051E-4	44.000004	12
TGAGGTA	20	6.952051E-4	44.000004	1
AGTTGGA	20	6.952051E-4	44.000004	21
CTTGCGG	20	6.952051E-4	44.000004	1
TTTGGTT	20	6.952051E-4	44.000004	3
TTGGTTA	20	6.952051E-4	44.000004	4
TTGCGGC	20	6.952051E-4	44.000004	2
TCTTTGG	20	6.952051E-4	44.000004	1
>>END_MODULE
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226529 spots for SRR5030975.sra
Written 226529 spots for SRR5030975.sra
Read 226530 spots for SRR5030975.sra
Written 226530 spots for SRR5030975.sra
SRR ids: ['SRR5030975.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xsf9kiva
SRR5030975.sra spots: 2718349
blocks: [[1, 226529], [226530, 453058], [453059, 679587], [679588, 906116], [906117, 1132645], [1132646, 1359174], [1359175, 1585703], [1585704, 1812232], [1812233, 2038761], [2038762, 2265290], [2265291, 2491819], [2491820, 2718349]]
SRR5030975 file size 470081
SRR5030975 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030975 SRR5030975_1.fastq
Input file:	SRR5030975_1.fastq
trimmed:	SRR5030975-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:57:58 2020 >> started

Wed Mar  4 06:58:00 2020 >> done (1.714s)
2718349 reads processed; of these:
    205 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
2718140 (99.99%) reads available; of these:
 184890 ( 6.80%) trimmed reads available after processing
2533250 (93.20%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     61	  0.00%
 19	    103	  0.00%
 20	    139	  0.01%
 21	    191	  0.01%
 22	    321	  0.01%
 23	    353	  0.01%
 24	    449	  0.02%
 25	    610	  0.02%
 26	    519	  0.02%
 27	    669	  0.02%
 28	    771	  0.03%
 29	   1058	  0.04%
 30	    682	  0.03%
 31	    717	  0.03%
 32	    788	  0.03%
 33	   1239	  0.05%
 34	   1513	  0.06%
 35	   2693	  0.10%
 36	   2509	  0.09%
 37	   2670	  0.10%
 38	   2804	  0.10%
 39	   3048	  0.11%
 40	   3607	  0.13%
 41	   3683	  0.14%
 42	   5026	  0.18%
 43	   6785	  0.25%
 44	   9657	  0.36%
 45	  12052	  0.44%
 46	  18964	  0.70%
 47	  21235	  0.78%
 48	  32188	  1.18%
 49	  47786	  1.76%
 50	2533250	 93.20%
2718140 reads passed initial QC


criterion=sequence-density
sequence-density=88.22
sequence-density-rank=1
fanout-score=20.45
fanout-score-rank=1
prefix-density=90.01
prefix-fanout=20.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC


criterion=fanout-score
sequence-density=88.22
sequence-density-rank=1
fanout-score=20.45
fanout-score-rank=1
prefix-density=90.01
prefix-fanout=20.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC -o SRR5030975 -
Input file:	STDIN
trimmed:	SRR5030975-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:58:14 2020 >> started

Wed Mar  4 06:58:18 2020 >> done (4.140s)
2657058 reads processed; of these:
 219954 ( 8.28%) short reads filtered out after trimming by size control
  13633 ( 0.51%) empty reads filtered out after trimming by size control
2423471 (91.21%) reads available; of these:
2332317 (96.24%) trimmed reads available after processing
  91154 ( 3.76%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16470	  0.68%
 19	  35134	  1.45%
 20	 158988	  6.56%
 21	 300881	 12.42%
 22	 760602	 31.38%
 23	 351383	 14.50%
 24	 156432	  6.45%
 25	  41935	  1.73%
 26	  23295	  0.96%
 27	  18238	  0.75%
 28	  21043	  0.87%
 29	  15441	  0.64%
 30	  21725	  0.90%
 31	  28754	  1.19%
 32	  30447	  1.26%
 33	  72174	  2.98%
 34	  35408	  1.46%
 35	  29087	  1.20%
 36	  33648	  1.39%
 37	  13849	  0.57%
 38	  15294	  0.63%
 39	  13222	  0.55%
 40	  18793	  0.78%
 41	  25259	  1.04%
 42	  28606	  1.18%
 43	  20375	  0.84%
 44	  13968	  0.58%
 45	  20380	  0.84%
 46	  15736	  0.65%
 47	  10344	  0.43%
 48	   1001	  0.04%
 49	   1633	  0.07%
 50	  73926	  3.05%


criterion=sequence-density
sequence-density=7.58
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.53
sequence-density-rank=27
fanout-score=15.04
fanout-score-rank=1
prefix-density=7.94
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:58:40
                             Started mapping on |	Mar 04 06:58:40
                                    Finished on |	Mar 04 06:59:06
       Mapping speed, Million of reads per hour |	344.02

                          Number of input reads |	2484553
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1287524
                        Uniquely mapped reads % |	51.82%
                          Average mapped length |	24.05
                       Number of splices: Total |	1382
            Number of splices: Annotated (sjdb) |	190
                       Number of splices: GT/AG |	1295
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	71
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.84
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	881728
             % of reads mapped to multiple loci |	35.49%
        Number of reads mapped to too many loci |	56671
             % of reads mapped to too many loci |	2.28%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.71%
                     % of reads unmapped: other |	0.70%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	315301	315301	315301
N_multimapping	881728	881728	881728
N_noFeature	63854	77654	857547
N_ambiguous	742978	368633	12266
UnstrandedReadsAssigned:480692 PositiveStrandReadsAssigned:841237 NegativeStrandReadsAssigned:417711
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030975 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030975-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,484,553 reads, 635,950 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,194 rounds

 109283 SRR5030975.ke.tsv
  52636 SRR5030975.se.tsv
 161919 total
==> SRR5030975.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030975.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030975 completed mapping pipeline successfully
