Starting /dee2/code/volunteer_pipeline.sh SRR5030865
    current disk space = 160677052416
    free memory = 73949880 
SRR5030865 SRAfilesize
9c149c7a115b9f003f70e46daef8ac88  SRR5030865.sra
SRR5030865.sra file validated
SRR5030865 is single end
SRR5030865 is conventional basespace
SRR5030865 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030865_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52925	34.0	31.0	34.0	31.0	34.0
2	32.70875	34.0	31.0	34.0	31.0	34.0
3	32.83	34.0	31.0	34.0	31.0	34.0
4	36.20375	37.0	37.0	37.0	35.0	37.0
5	36.302	37.0	37.0	37.0	35.0	37.0
6	36.11175	37.0	36.0	37.0	35.0	37.0
7	36.10875	37.0	35.0	37.0	35.0	37.0
8	36.20325	37.0	36.0	37.0	35.0	37.0
9	38.00275	39.0	38.0	39.0	35.0	39.0
10	37.99475	39.0	39.0	39.0	35.0	39.0
11	37.97475	39.0	38.0	39.0	35.0	39.0
12	37.9205	39.0	38.0	39.0	35.0	39.0
13	37.879	39.0	38.0	39.0	35.0	39.0
14	39.30525	41.0	39.0	41.0	36.0	41.0
15	39.1955	40.0	39.0	41.0	36.0	41.0
16	39.221	40.0	39.0	41.0	36.0	41.0
17	39.4245	41.0	39.0	41.0	36.0	41.0
18	39.35325	41.0	39.0	41.0	36.0	41.0
19	39.2225	41.0	39.0	41.0	36.0	41.0
20	39.176	41.0	39.0	41.0	36.0	41.0
21	39.31925	41.0	39.0	41.0	36.0	41.0
22	39.213	41.0	39.0	41.0	36.0	41.0
23	39.14875	41.0	39.0	41.0	36.0	41.0
24	38.83975	40.0	39.0	41.0	35.0	41.0
25	39.0335	41.0	39.0	41.0	35.0	41.0
26	39.033	40.0	39.0	41.0	35.0	41.0
27	39.0895	41.0	39.0	41.0	35.0	41.0
28	38.83625	40.0	38.0	41.0	35.0	41.0
29	38.172	40.0	38.0	41.0	33.0	41.0
30	38.503	40.0	38.0	41.0	34.0	41.0
31	38.845	40.0	38.0	41.0	35.0	41.0
32	39.04325	40.0	39.0	41.0	35.0	41.0
33	38.23025	40.0	38.0	41.0	34.0	41.0
34	38.1505	40.0	38.0	41.0	33.0	41.0
35	38.03775	40.0	37.0	41.0	34.0	41.0
36	34.3235	38.0	31.0	40.0	23.0	41.0
37	35.6295	38.0	34.0	40.0	30.0	41.0
38	37.04825	39.0	37.0	40.0	31.0	41.0
39	36.96275	39.0	36.0	40.0	31.0	41.0
40	36.9655	39.0	36.0	40.0	31.0	41.0
41	37.00475	39.0	36.0	40.0	31.0	41.0
42	37.43425	40.0	37.0	41.0	33.0	41.0
43	37.24925	40.0	37.0	41.0	32.0	41.0
44	37.037	40.0	37.0	41.0	31.0	41.0
45	36.827	39.0	36.0	41.0	31.0	41.0
46	35.132	38.0	34.0	40.0	26.0	41.0
47	35.658	38.0	35.0	40.0	28.0	41.0
48	35.859	39.0	35.0	40.0	29.0	41.0
49	34.74675	38.0	34.0	40.0	27.0	41.0
50	30.3485	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	1.0
19	2.0
20	1.0
21	5.0
22	5.0
23	6.0
24	11.0
25	11.0
26	21.0
27	31.0
28	26.0
29	35.0
30	52.0
31	60.0
32	87.0
33	119.0
34	161.0
35	206.0
36	332.0
37	483.0
38	1056.0
39	1286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.13259944958719	22.34175631723793	51.86389792344258	15.6617463097323
2	23.150000000000002	23.599999999999998	20.175	33.074999999999996
3	26.474999999999998	18.925	21.925	32.675
4	33.175	25.474999999999998	14.725	26.625
5	19.900000000000002	42.825	24.25	13.025
6	34.225	15.775	34.699999999999996	15.299999999999999
7	18.35	19.375	24.75	37.525
8	25.8	28.050000000000004	15.75	30.4
9	23.075000000000003	33.95	27.575	15.4
10	21.725	22.625	29.925	25.724999999999998
11	28.65	12.875	16.975	41.5
12	15.85	19.825	31.7	32.625
13	31.424999999999997	18.75	31.7	18.125
14	27.675	32.800000000000004	22.15	17.375
15	35.20880220055014	24.956239059764943	25.03125781445361	14.803700925231308
16	19.400000000000002	20.625	21.65	38.324999999999996
17	20.575	23.525	40.375	15.525
18	22.2	18.475	31.25	28.075
19	37.824999999999996	13.8	29.275000000000002	19.1
20	40.525	15.65	30.075000000000003	13.750000000000002
21	24.275	17.2	28.499999999999996	30.025000000000002
22	29.225	12.975	46.625	11.175
23	28.025	13.55	42.825	15.6
24	52.65	15.0	23.575	8.774999999999999
25	47.675	26.75	13.825000000000001	11.75
26	26.325	45.525	14.674999999999999	13.475000000000001
27	15.675	46.225	25.2	12.9
28	13.225000000000001	22.85	46.975	16.950000000000003
29	12.425	12.6	55.025	19.950000000000003
30	10.575	7.55	34.075	47.8
31	15.15	6.575	46.150000000000006	32.125
32	30.2	7.2749999999999995	20.150000000000002	42.375
33	53.65	10.6	12.8	22.95
34	58.525000000000006	8.3	18.275	14.899999999999999
35	62.125	8.35	19.25	10.274999999999999
36	42.3	9.375	34.275	14.05
37	43.4	10.9	20.599999999999998	25.1
38	23.05	17.4	12.125	47.425
39	15.25	26.325	10.875	47.55
40	16.125	48.6	12.55	22.725
41	26.400000000000002	47.225	9.775	16.6
42	49.3	28.4	11.899999999999999	10.4
43	47.825	30.45	10.5	11.225
44	28.975	47.949999999999996	7.775	15.299999999999999
45	17.95	48.275	14.75	19.025
46	13.925	24.75	19.775000000000002	41.55
47	10.025	15.275	39.7	35.0
48	11.825	13.350000000000001	20.575	54.25
49	15.174999999999999	20.599999999999998	13.875000000000002	50.349999999999994
50	20.5	35.925000000000004	13.950000000000001	29.625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	1.5
30	3.0
31	9.5
32	16.0
33	21.5
34	27.0
35	38.0
36	49.0
37	60.0
38	71.0
39	60.5
40	50.0
41	52.5
42	55.0
43	78.0
44	101.0
45	163.5
46	226.0
47	316.0
48	406.0
49	530.0
50	654.0
51	710.5
52	767.0
53	590.0
54	413.0
55	427.5
56	442.0
57	370.0
58	298.0
59	188.5
60	79.0
61	66.0
62	53.0
63	98.5
64	144.0
65	87.5
66	31.0
67	24.0
68	17.0
69	19.5
70	22.0
71	40.5
72	59.0
73	33.0
74	7.0
75	4.0
76	1.0
77	2.0
78	3.0
79	3.0
80	3.0
81	2.0
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21902377972467	30.049999999999997
2	10.638297872340425	8.5
3	4.06758448060075	4.875
4	1.877346683354193	3.0
5	1.2515644555694618	2.5
6	1.2515644555694618	3.0
7	0.7509386733416771	2.1
8	0.6257822277847309	2.0
9	0.5006257822277848	1.7999999999999998
>10	3.1914893617021276	21.125
>50	0.4380475594493116	12.575
>100	0.18773466833541927	8.475000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	116	2.9000000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	112	2.8000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	111	2.775	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	92	2.3	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	79	1.975	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	78	1.95	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	73	1.825	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	48	1.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	25	0.625	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	21	0.525	RNA PCR Primer, Index 2 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	19	0.475	RNA PCR Primer, Index 2 (100% over 50bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	17	0.42500000000000004	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	16	0.4	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	16	0.4	RNA PCR Primer, Index 2 (100% over 35bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	11	0.27499999999999997	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	10	0.25	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	10	0.25	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	10	0.25	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	10	0.25	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	10	0.25	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	10	0.25	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	9	0.22499999999999998	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	7	0.17500000000000002	RNA PCR Primer, Index 2 (100% over 35bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	6	0.15	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	6	0.15	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTAT	6	0.15	RNA PCR Primer, Index 2 (100% over 48bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	5	0.125	No Hit
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCGTTCGCGCTCTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.55	0.0	0.0	0.0
2	0.0	0.575	0.0	0.0	0.0
3	0.0	0.725	0.0	0.0	0.0
4	0.0	0.75	0.0	0.0	0.0
5	0.0	0.8	0.0	0.0	0.0
6	0.0	0.95	0.0	0.0	0.0
7	0.0	1.6	0.0	0.0	0.0
8	0.0	1.75	0.0	0.0	0.0
9	0.0	1.875	0.0	0.0	0.0
10	0.0	2.2	0.0	0.0	0.0
11	0.0	2.275	0.0	0.0	0.0
12	0.0	2.65	0.0	0.0	0.0
13	0.0	2.9	0.0	0.0	0.0
14	0.0	3.225	0.0	0.0	0.0
15	0.0	3.625	0.0	0.0	0.0
16	0.0	4.75	0.0	0.0	0.0
17	0.0	5.375	0.0	0.0	0.0
18	0.0	6.525	0.0	0.0	0.0
19	0.0	7.275	0.0	0.0	0.0
20	0.0	7.975	0.0	0.0	0.0
21	0.0	14.0	0.0	0.0	0.0
22	0.0	25.5	0.0	0.0	0.0
23	0.0	53.725	0.0	0.0	0.0
24	0.0	64.85	0.0	0.0	0.0
25	0.0	69.65	0.0	0.0	0.0
26	0.0	70.85	0.0	0.0	0.0
27	0.0	71.575	0.0	0.0	0.0
28	0.0	72.0	0.0	0.0	0.0
29	0.0	72.45	0.0	0.0	0.0
30	0.0	72.8	0.0	0.0	0.0
31	0.0	73.4	0.0	0.0	0.0
32	0.0	74.45	0.0	0.0	0.0
33	0.0	74.975	0.0	0.0	0.0
34	0.0	78.225	0.0	0.0	0.0
35	0.0	79.15	0.0	0.0	0.0
36	0.0	79.825	0.0	0.0	0.0
37	0.0	80.85	0.0	0.0	0.0
38	0.0	81.225	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACAT	20	6.952051E-4	44.000004	3
AAACATC	20	6.952051E-4	44.000004	4
ACATCCT	20	6.952051E-4	44.000004	6
CTTGGCT	40	5.315087E-9	44.000004	16
AGGCTTG	20	6.952051E-4	44.000004	18
GGCTTGG	45	2.728484E-10	44.000004	19
GCTTGGC	40	5.315087E-9	44.000004	15
CTGTTGG	20	6.952051E-4	44.000004	19
TGTAAAC	20	6.952051E-4	44.000004	1
GTAAACA	20	6.952051E-4	44.000004	2
AACATCC	20	6.952051E-4	44.000004	5
AAGTAAT	25	3.6958358E-5	44.0	4
TCAAGTA	25	3.6958358E-5	44.0	2
AATCCAG	25	3.6958358E-5	44.0	8
CTGAGCT	55	0.0	44.0	11
ATCCAGG	25	3.6958358E-5	44.0	9
TTCAAGT	25	3.6958358E-5	44.0	1
ATAGGCT	25	3.6958358E-5	44.0	16
GAGCTTG	55	0.0	44.0	13
GGATCCG	55	0.0	44.0	3
>>END_MODULE
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240621 spots for SRR5030865.sra
Written 240621 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
Read 240611 spots for SRR5030865.sra
Written 240611 spots for SRR5030865.sra
SRR ids: ['SRR5030865.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5gnnesqn
SRR5030865.sra spots: 2887342
blocks: [[1, 240611], [240612, 481222], [481223, 721833], [721834, 962444], [962445, 1203055], [1203056, 1443666], [1443667, 1684277], [1684278, 1924888], [1924889, 2165499], [2165500, 2406110], [2406111, 2646721], [2646722, 2887342]]
SRR5030865 file size 499364
SRR5030865 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030865 SRR5030865_1.fastq
Input file:	SRR5030865_1.fastq
trimmed:	SRR5030865-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:31:51 2020 >> started

Wed Mar  4 06:31:53 2020 >> done (1.683s)
2887342 reads processed; of these:
    139 ( 0.00%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
2887195 (99.99%) reads available; of these:
 166936 ( 5.78%) trimmed reads available after processing
2720259 (94.22%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     43	  0.00%
 19	     64	  0.00%
 20	    114	  0.00%
 21	    113	  0.00%
 22	    206	  0.01%
 23	    255	  0.01%
 24	    293	  0.01%
 25	    427	  0.01%
 26	    285	  0.01%
 27	    412	  0.01%
 28	    536	  0.02%
 29	    651	  0.02%
 30	    515	  0.02%
 31	    786	  0.03%
 32	    790	  0.03%
 33	   1113	  0.04%
 34	   2248	  0.08%
 35	   2517	  0.09%
 36	   1832	  0.06%
 37	   1967	  0.07%
 38	   2254	  0.08%
 39	   2557	  0.09%
 40	   2714	  0.09%
 41	   3151	  0.11%
 42	   3623	  0.13%
 43	   5240	  0.18%
 44	   9070	  0.31%
 45	  11981	  0.41%
 46	  12310	  0.43%
 47	  16839	  0.58%
 48	  35155	  1.22%
 49	  46875	  1.62%
 50	2720259	 94.22%
2887195 reads passed initial QC


criterion=sequence-density
sequence-density=81.63
sequence-density-rank=1
fanout-score=19.64
fanout-score-rank=1
prefix-density=83.26
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC


criterion=fanout-score
sequence-density=81.63
sequence-density-rank=1
fanout-score=19.64
fanout-score-rank=1
prefix-density=83.26
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC -o SRR5030865 -
Input file:	STDIN
trimmed:	SRR5030865-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:32:06 2020 >> started

Wed Mar  4 06:32:10 2020 >> done (3.702s)
2816776 reads processed; of these:
 175486 ( 6.23%) short reads filtered out after trimming by size control
  12311 ( 0.44%) empty reads filtered out after trimming by size control
2628979 (93.33%) reads available; of these:
2336475 (88.87%) trimmed reads available after processing
 292504 (11.13%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  18264	  0.69%
 19	  29373	  1.12%
 20	 158233	  6.02%
 21	 331136	 12.60%
 22	 796471	 30.30%
 23	 326481	 12.42%
 24	 145752	  5.54%
 25	  36232	  1.38%
 26	  19202	  0.73%
 27	  16893	  0.64%
 28	  16847	  0.64%
 29	  13289	  0.51%
 30	  19295	  0.73%
 31	  27096	  1.03%
 32	  22659	  0.86%
 33	  94741	  3.60%
 34	  28097	  1.07%
 35	  21893	  0.83%
 36	  26428	  1.01%
 37	  12917	  0.49%
 38	  13257	  0.50%
 39	  13398	  0.51%
 40	  18318	  0.70%
 41	  23926	  0.91%
 42	  27432	  1.04%
 43	  20878	  0.79%
 44	  17428	  0.66%
 45	  23244	  0.88%
 46	  18498	  0.70%
 47	  15874	  0.60%
 48	   2888	  0.11%
 49	   8897	  0.34%
 50	 263642	 10.03%


criterion=sequence-density
sequence-density=4.61
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=19
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=22
fanout-score=14.84
fanout-score-rank=1
prefix-density=7.96
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTTT
                                 Started job on |	Mar 04 06:32:30
                             Started mapping on |	Mar 04 06:32:31
                                    Finished on |	Mar 04 06:32:55
       Mapping speed, Million of reads per hour |	404.91

                          Number of input reads |	2699398
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1483558
                        Uniquely mapped reads % |	54.96%
                          Average mapped length |	26.72
                       Number of splices: Total |	1413
            Number of splices: Annotated (sjdb) |	233
                       Number of splices: GT/AG |	1315
                       Number of splices: GC/AG |	27
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	71
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.58
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	848209
             % of reads mapped to multiple loci |	31.42%
        Number of reads mapped to too many loci |	61911
             % of reads mapped to too many loci |	2.29%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.81%
                     % of reads unmapped: other |	0.52%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	367631	367631	367631
N_multimapping	848209	848209	848209
N_noFeature	72395	87085	1025783
N_ambiguous	841470	440379	16860
UnstrandedReadsAssigned:569693 PositiveStrandReadsAssigned:956094 NegativeStrandReadsAssigned:440915
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030865 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030865-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,699,398 reads, 713,117 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,127 rounds

 109283 SRR5030865.ke.tsv
  52636 SRR5030865.se.tsv
 161919 total
==> SRR5030865.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030865.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030865 completed mapping pipeline successfully
