Starting /dee2/code/volunteer_pipeline.sh SRR5030869
    current disk space = 160664256512
    free memory = 73952660 
SRR5030869 SRAfilesize
3b0bd63393ff106b719fab0d5050fbfe  SRR5030869.sra
SRR5030869.sra file validated
SRR5030869 is single end
SRR5030869 is conventional basespace
SRR5030869 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.354	33.0	31.0	34.0	31.0	34.0
2	32.70975	34.0	31.0	34.0	31.0	34.0
3	32.82375	34.0	31.0	34.0	31.0	34.0
4	36.2515	37.0	37.0	37.0	35.0	37.0
5	36.31675	37.0	37.0	37.0	35.0	37.0
6	36.0235	37.0	35.0	37.0	35.0	37.0
7	36.01	37.0	35.0	37.0	35.0	37.0
8	36.07575	37.0	35.0	37.0	35.0	37.0
9	37.877	39.0	38.0	39.0	35.0	39.0
10	37.86775	39.0	38.0	39.0	35.0	39.0
11	37.68025	39.0	38.0	39.0	35.0	39.0
12	37.82025	39.0	38.0	39.0	35.0	39.0
13	37.7105	39.0	38.0	39.0	35.0	39.0
14	39.2585	41.0	39.0	41.0	36.0	41.0
15	39.16175	40.0	39.0	41.0	36.0	41.0
16	39.155	40.0	39.0	41.0	36.0	41.0
17	39.33475	41.0	39.0	41.0	36.0	41.0
18	39.28525	41.0	39.0	41.0	36.0	41.0
19	39.0785	41.0	39.0	41.0	35.0	41.0
20	39.04225	40.0	39.0	41.0	35.0	41.0
21	39.16325	41.0	39.0	41.0	36.0	41.0
22	39.162	41.0	39.0	41.0	36.0	41.0
23	39.01975	40.0	39.0	41.0	35.0	41.0
24	38.73475	40.0	38.0	41.0	35.0	41.0
25	38.96525	41.0	39.0	41.0	35.0	41.0
26	39.05	41.0	39.0	41.0	35.0	41.0
27	39.0955	41.0	39.0	41.0	35.0	41.0
28	38.816	40.0	38.0	41.0	35.0	41.0
29	37.90125	40.0	38.0	41.0	32.0	41.0
30	38.0065	40.0	38.0	41.0	33.0	41.0
31	38.39825	40.0	38.0	41.0	34.0	41.0
32	38.693	40.0	38.0	41.0	34.0	41.0
33	37.662	40.0	38.0	41.0	32.0	41.0
34	37.84175	40.0	38.0	41.0	33.0	41.0
35	37.69875	40.0	37.0	41.0	33.0	41.0
36	34.8195	38.0	33.0	40.0	23.0	41.0
37	35.846	38.0	35.0	40.0	30.0	41.0
38	36.92725	39.0	36.0	40.0	31.0	41.0
39	37.01	39.0	37.0	40.0	31.0	41.0
40	36.97475	39.0	37.0	40.0	31.0	41.0
41	36.894	39.0	36.0	41.0	31.0	41.0
42	37.097	40.0	36.0	41.0	32.0	41.0
43	36.947	40.0	36.0	41.0	31.0	41.0
44	36.96325	39.0	36.0	41.0	32.0	41.0
45	36.522	39.0	36.0	40.0	31.0	41.0
46	34.9325	38.0	34.0	40.0	26.0	41.0
47	35.2865	38.0	34.0	40.0	28.0	41.0
48	35.38075	39.0	35.0	40.0	28.0	41.0
49	34.6	38.0	34.0	40.0	26.0	41.0
50	30.262	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	3.0
18	1.0
19	3.0
20	7.0
21	8.0
22	10.0
23	4.0
24	12.0
25	23.0
26	26.0
27	26.0
28	25.0
29	41.0
30	49.0
31	74.0
32	92.0
33	96.0
34	180.0
35	201.0
36	346.0
37	490.0
38	978.0
39	1304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.714643304130162	24.58072590738423	46.03254067584481	17.672090112640802
2	25.324999999999996	24.95	20.674999999999997	29.049999999999997
3	24.0	20.1	22.05	33.85
4	33.125	26.8	15.174999999999999	24.9
5	21.65	40.300000000000004	25.924999999999997	12.125
6	32.375	16.675	36.4	14.549999999999999
7	19.625	20.5	22.85	37.025000000000006
8	25.174999999999997	29.675	16.3	28.849999999999998
9	20.45	33.625	28.725	17.2
10	23.225	21.125	30.349999999999998	25.3
11	32.324999999999996	14.274999999999999	15.7	37.7
12	16.575	22.175	29.725	31.525
13	29.95	19.8	31.5	18.75
14	28.65	30.4	23.425	17.525
15	33.96698349174587	24.862431215607803	25.312656328164078	15.857928964482241
16	21.05	20.0	22.0	36.95
17	23.025000000000002	24.175	37.35	15.45
18	23.25	19.2	30.95	26.6
19	35.625	15.0	31.900000000000002	17.474999999999998
20	40.675	16.900000000000002	28.499999999999996	13.925
21	25.2	19.55	26.25	28.999999999999996
22	28.575	13.05	45.0	13.375
23	26.875	13.900000000000002	42.05	17.175
24	50.975	13.725000000000001	24.25	11.05
25	47.5	25.15	14.325	13.025
26	27.224999999999998	43.15	14.899999999999999	14.725
27	17.474999999999998	44.0	23.974999999999998	14.549999999999999
28	14.000000000000002	23.825	45.050000000000004	17.125
29	13.4	13.350000000000001	52.800000000000004	20.45
30	10.9	8.25	35.675000000000004	45.175
31	15.625	6.8500000000000005	44.25	33.275
32	29.775000000000002	6.875000000000001	22.3	41.05
33	50.449999999999996	11.825	13.575000000000001	24.15
34	56.775	8.674999999999999	18.475	16.075
35	59.475	9.375	19.775000000000002	11.375
36	44.15	9.700000000000001	32.75	13.4
37	41.9	12.875	21.45	23.775
38	24.0	17.275	13.5	45.225
39	17.275	24.275	13.25	45.2
40	16.5	45.050000000000004	14.149999999999999	24.3
41	25.650000000000002	45.35	10.975	18.025
42	47.775	28.199999999999996	13.375	10.65
43	49.275000000000006	29.549999999999997	8.924999999999999	12.25
44	30.15	46.2	9.75	13.900000000000002
45	19.55	47.4	13.575000000000001	19.475
46	16.525000000000002	26.025	19.325	38.125
47	10.8	15.775	37.875	35.55
48	13.775	13.4	20.4	52.425
49	14.75	20.825	14.6	49.825
50	20.65	34.1	14.2	31.05
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.5
26	2.0
27	3.0
28	4.0
29	5.0
30	6.0
31	8.0
32	10.0
33	20.0
34	30.0
35	37.0
36	44.0
37	57.5
38	71.0
39	76.0
40	81.0
41	71.0
42	61.0
43	107.0
44	153.0
45	192.0
46	231.0
47	297.5
48	364.0
49	473.5
50	583.0
51	656.5
52	730.0
53	549.0
54	368.0
55	428.0
56	488.0
57	381.5
58	275.0
59	191.0
60	107.0
61	81.0
62	55.0
63	110.5
64	166.0
65	106.5
66	47.0
67	31.5
68	16.0
69	24.5
70	33.0
71	44.5
72	56.0
73	31.0
74	6.0
75	4.0
76	2.0
77	4.5
78	7.0
79	4.5
80	2.0
81	1.0
82	0.0
83	0.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.05
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15812229771464	30.825000000000003
2	9.512044471896232	7.7
3	3.211859172328598	3.9
4	3.026559604694256	4.9
5	1.4823965410747375	3.0
6	1.5441630636195183	3.75
7	0.6176652254478072	1.7500000000000002
8	0.5558987029030266	1.7999999999999998
9	0.4323656578134651	1.575
>10	2.9030265596046942	21.575
>50	0.24706609017912293	5.55
>100	0.3088326127239036	13.675
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	121	3.025	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	109	2.725	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	108	2.7	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	106	2.65	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	59	1.4749999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	57	1.425	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	55	1.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	42	1.05	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	42	1.05	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	37	0.9249999999999999	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	31	0.775	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	24	0.6	RNA PCR Primer, Index 7 (100% over 35bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	23	0.575	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	22	0.5499999999999999	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	16	0.4	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	14	0.35000000000000003	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	14	0.35000000000000003	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	13	0.325	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	13	0.325	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTC	12	0.3	RNA PCR Primer, Index 33 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	12	0.3	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	11	0.27499999999999997	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	11	0.27499999999999997	No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 33 (100% over 50bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	9	0.22499999999999998	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	9	0.22499999999999998	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	9	0.22499999999999998	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	8	0.2	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	7	0.17500000000000002	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	7	0.17500000000000002	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	7	0.17500000000000002	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	7	0.17500000000000002	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	6	0.15	RNA PCR Primer, Index 1 (96% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	6	0.15	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	5	0.125	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	5	0.125	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.25	0.0	0.0	0.0
3	0.0	0.375	0.0	0.0	0.0
4	0.0	0.425	0.0	0.0	0.0
5	0.0	0.475	0.0	0.0	0.0
6	0.0	0.55	0.0	0.0	0.0
7	0.0	0.95	0.0	0.0	0.0
8	0.0	1.125	0.0	0.0	0.0
9	0.0	1.125	0.0	0.0	0.0
10	0.0	1.2	0.0	0.0	0.0
11	0.0	1.325	0.0	0.0	0.0
12	0.0	1.5	0.0	0.0	0.0
13	0.0	1.75	0.0	0.0	0.0
14	0.0	1.925	0.0	0.0	0.0
15	0.0	2.275	0.0	0.0	0.0
16	0.0	3.25	0.0	0.0	0.0
17	0.0	3.8	0.0	0.0	0.0
18	0.0	4.65	0.0	0.0	0.0
19	0.0	5.1	0.0	0.0	0.0
20	0.0	6.125	0.0	0.0	0.0
21	0.0	10.6	0.0	0.0	0.0
22	0.0	21.35	0.0	0.0	0.0
23	0.0	46.825	0.0	0.0	0.0
24	0.0	58.625	0.0	0.0	0.0
25	0.0	63.525	0.0	0.0	0.0
26	0.0	64.825	0.0	0.0	0.0
27	0.0	65.6	0.0	0.0	0.0
28	0.0	66.025	0.0	0.0	0.0
29	0.0	66.675	0.0	0.0	0.0
30	0.0	67.1	0.0	0.0	0.0
31	0.0	68.075	0.0	0.0	0.0
32	0.0	69.45	0.0	0.0	0.0
33	0.0	70.375	0.0	0.0	0.0
34	0.0	74.275	0.0	0.0	0.0
35	0.0	75.325	0.0	0.0	0.0
36	0.0	76.025	0.0	0.0	0.0
37	0.0	77.025	0.0	0.0	0.0
38	0.0	77.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCG	20	6.952051E-4	44.000004	8
TCCTCGA	20	6.952051E-4	44.000004	9
GGTGAGT	20	6.952051E-4	44.000004	17
TCGACTG	20	6.952051E-4	44.000004	12
CATCCTC	20	6.952051E-4	44.000004	7
CCTCGAC	20	6.952051E-4	44.000004	10
GGCTTTG	20	6.952051E-4	44.000004	19
CGGTGAG	20	6.952051E-4	44.000004	16
CTGTTGG	20	6.952051E-4	44.000004	19
GCTTTGG	20	6.952051E-4	44.000004	20
CGACTGG	20	6.952051E-4	44.000004	13
AACGCTG	35	1.02281774E-7	44.0	8
ACGCTGT	35	1.02281774E-7	44.0	9
TAAACAT	30	1.9515464E-6	44.0	3
AAACATC	30	1.9515464E-6	44.0	4
TTCAACG	35	1.02281774E-7	44.0	5
TCGGTGA	35	1.02281774E-7	44.0	15
GAGCTTG	35	1.02281774E-7	44.0	13
GGATCCG	35	1.02281774E-7	44.0	3
TCGGATC	35	1.02281774E-7	44.0	1
>>END_MODULE
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114017 spots for SRR5030869.sra
Written 114017 spots for SRR5030869.sra
Read 114023 spots for SRR5030869.sra
Written 114023 spots for SRR5030869.sra
SRR ids: ['SRR5030869.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_olbrpiaa
SRR5030869.sra spots: 1368210
blocks: [[1, 114017], [114018, 228034], [228035, 342051], [342052, 456068], [456069, 570085], [570086, 684102], [684103, 798119], [798120, 912136], [912137, 1026153], [1026154, 1140170], [1140171, 1254187], [1254188, 1368210]]
SRR5030869 file size 236060
SRR5030869 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030869 SRR5030869_1.fastq
Input file:	SRR5030869_1.fastq
trimmed:	SRR5030869-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:32:42 2020 >> started

Wed Mar  4 06:32:43 2020 >> done (0.937s)
1368210 reads processed; of these:
     81 ( 0.01%) short reads filtered out after trimming by size control
      2 ( 0.00%) empty reads filtered out after trimming by size control
1368127 (99.99%) reads available; of these:
  84085 ( 6.15%) trimmed reads available after processing
1284042 (93.85%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     16	  0.00%
 19	     31	  0.00%
 20	     55	  0.00%
 21	     78	  0.01%
 22	    112	  0.01%
 23	    167	  0.01%
 24	    175	  0.01%
 25	    235	  0.02%
 26	    205	  0.01%
 27	    278	  0.02%
 28	    351	  0.03%
 29	    446	  0.03%
 30	    354	  0.03%
 31	    467	  0.03%
 32	    448	  0.03%
 33	    532	  0.04%
 34	   1189	  0.09%
 35	   1066	  0.08%
 36	    887	  0.06%
 37	    932	  0.07%
 38	   1205	  0.09%
 39	   1321	  0.10%
 40	   1505	  0.11%
 41	   1730	  0.13%
 42	   2143	  0.16%
 43	   2878	  0.21%
 44	   4711	  0.34%
 45	   6302	  0.46%
 46	   5888	  0.43%
 47	   8507	  0.62%
 48	  15745	  1.15%
 49	  24126	  1.76%
 50	1284042	 93.85%
1368127 reads passed initial QC


criterion=sequence-density
sequence-density=76.70
sequence-density-rank=1
fanout-score=20.65
fanout-score-rank=1
prefix-density=78.89
prefix-fanout=20.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCC


criterion=fanout-score
sequence-density=76.70
sequence-density-rank=1
fanout-score=20.65
fanout-score-rank=1
prefix-density=78.89
prefix-fanout=20.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCC -o SRR5030869 -
Input file:	STDIN
trimmed:	SRR5030869-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:32:52 2020 >> started

Wed Mar  4 06:32:54 2020 >> done (1.547s)
1332591 reads processed; of these:
  64685 ( 4.85%) short reads filtered out after trimming by size control
   4333 ( 0.33%) empty reads filtered out after trimming by size control
1263573 (94.82%) reads available; of these:
1076651 (85.21%) trimmed reads available after processing
 186922 (14.79%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   6878	  0.54%
 19	  12177	  0.96%
 20	  58842	  4.66%
 21	 136190	 10.78%
 22	 358992	 28.41%
 23	 156357	 12.37%
 24	  67341	  5.33%
 25	  17469	  1.38%
 26	   9071	  0.72%
 27	   7937	  0.63%
 28	   8538	  0.68%
 29	   6812	  0.54%
 30	   9963	  0.79%
 31	  15592	  1.23%
 32	  12303	  0.97%
 33	  50115	  3.97%
 34	  14225	  1.13%
 35	  11417	  0.90%
 36	  14258	  1.13%
 37	   6884	  0.54%
 38	   7428	  0.59%
 39	   7219	  0.57%
 40	  10475	  0.83%
 41	  13463	  1.07%
 42	  15624	  1.24%
 43	  11550	  0.91%
 44	   9313	  0.74%
 45	  12237	  0.97%
 46	   9801	  0.78%
 47	   9246	  0.73%
 48	   1991	  0.16%
 49	   6172	  0.49%
 50	 167693	 13.27%


criterion=sequence-density
sequence-density=5.81
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.22
sequence-density-rank=5
fanout-score=19.68
fanout-score-rank=1
prefix-density=2.27
prefix-fanout=19.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGGCGATCTCGTATGC
                                 Started job on |	Mar 04 06:33:12
                             Started mapping on |	Mar 04 06:33:13
                                    Finished on |	Mar 04 06:33:35
       Mapping speed, Million of reads per hour |	212.58

                          Number of input reads |	1299109
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	688321
                        Uniquely mapped reads % |	52.98%
                          Average mapped length |	28.15
                       Number of splices: Total |	577
            Number of splices: Annotated (sjdb) |	109
                       Number of splices: GT/AG |	528
                       Number of splices: GC/AG |	4
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	45
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.69
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	407608
             % of reads mapped to multiple loci |	31.38%
        Number of reads mapped to too many loci |	33673
             % of reads mapped to too many loci |	2.59%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.52%
                     % of reads unmapped: other |	0.52%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	203180	203180	203180
N_multimapping	407608	407608	407608
N_noFeature	37480	45467	498142
N_ambiguous	386500	223075	8603
UnstrandedReadsAssigned:264341 PositiveStrandReadsAssigned:419779 NegativeStrandReadsAssigned:181576
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030869 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030869-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,299,109 reads, 317,611 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 887 rounds

 109283 SRR5030869.ke.tsv
  52636 SRR5030869.se.tsv
 161919 total
==> SRR5030869.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030869.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030869 completed mapping pipeline successfully
