Starting /dee2/code/volunteer_pipeline.sh SRR5030831
    current disk space = 160646791168
    free memory = 1054490320 
SRR5030831 SRAfilesize
009f2841e9dcf210ea7c4b90b2bb6ea0  SRR5030831.sra
SRR5030831.sra file validated
SRR5030831 is single end
SRR5030831 is conventional basespace
SRR5030831 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2745	33.0	31.0	34.0	31.0	34.0
2	32.678	34.0	31.0	34.0	31.0	34.0
3	32.727	34.0	31.0	34.0	31.0	34.0
4	36.24975	37.0	37.0	37.0	35.0	37.0
5	36.191	37.0	35.0	37.0	35.0	37.0
6	35.89225	37.0	35.0	37.0	35.0	37.0
7	36.032	37.0	35.0	37.0	35.0	37.0
8	36.06375	37.0	35.0	37.0	35.0	37.0
9	37.83525	39.0	38.0	39.0	35.0	39.0
10	37.8	39.0	38.0	39.0	35.0	39.0
11	37.73325	39.0	37.0	39.0	35.0	39.0
12	37.67775	39.0	37.0	39.0	35.0	39.0
13	37.6795	39.0	37.0	39.0	35.0	39.0
14	39.04575	40.0	38.0	41.0	35.0	41.0
15	39.045	40.0	38.0	41.0	35.0	41.0
16	39.0215	40.0	38.0	41.0	35.0	41.0
17	39.146	40.0	38.0	41.0	36.0	41.0
18	39.062	40.0	38.0	41.0	35.0	41.0
19	38.7025	40.0	38.0	41.0	34.0	41.0
20	38.922	40.0	38.0	41.0	35.0	41.0
21	39.0705	40.0	39.0	41.0	35.0	41.0
22	39.04675	40.0	39.0	41.0	36.0	41.0
23	39.04775	40.0	38.0	41.0	36.0	41.0
24	38.35125	40.0	38.0	41.0	34.0	41.0
25	38.538	40.0	38.0	41.0	34.0	41.0
26	38.59525	40.0	38.0	41.0	34.0	41.0
27	38.591	40.0	38.0	41.0	34.0	41.0
28	38.62225	40.0	38.0	41.0	34.0	41.0
29	38.21975	40.0	38.0	41.0	33.0	41.0
30	38.1805	40.0	38.0	41.0	33.0	41.0
31	38.56425	40.0	38.0	41.0	34.0	41.0
32	38.67375	40.0	38.0	41.0	35.0	41.0
33	37.07475	40.0	36.0	41.0	31.0	41.0
34	36.29275	39.0	36.0	41.0	29.0	41.0
35	32.1845	37.0	30.0	40.0	9.0	40.0
36	29.40525	36.0	21.0	40.0	8.0	40.0
37	29.4615	35.0	23.0	39.0	6.0	40.0
38	32.078	37.0	30.0	39.0	13.0	40.0
39	31.63575	37.0	30.0	39.0	7.0	41.0
40	32.25675	37.0	31.0	39.0	2.0	41.0
41	29.9505	36.0	26.0	40.0	2.0	41.0
42	31.604	37.0	30.0	40.0	2.0	41.0
43	29.65475	36.0	26.0	40.0	2.0	41.0
44	30.5575	37.0	30.0	40.0	2.0	41.0
45	28.68525	36.0	23.0	40.0	2.0	41.0
46	26.4475	34.0	8.0	39.0	2.0	41.0
47	26.4235	33.0	9.0	39.0	2.0	41.0
48	27.125	34.0	2.0	39.0	2.0	41.0
49	25.3085	34.0	2.0	39.0	2.0	41.0
50	22.062	29.0	2.0	37.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	2.0
20	10.0
21	17.0
22	27.0
23	34.0
24	41.0
25	68.0
26	99.0
27	155.0
28	64.0
29	115.0
30	172.0
31	167.0
32	194.0
33	220.0
34	248.0
35	282.0
36	314.0
37	466.0
38	611.0
39	690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.926835379604109	25.557504384865947	47.33149586569782	15.184164369832123
2	24.725	27.0	20.525	27.750000000000004
3	21.525	23.35	23.3	31.825
4	29.599999999999998	27.700000000000003	15.675	27.025
5	23.275000000000002	37.824999999999996	26.650000000000002	12.25
6	34.55	16.825000000000003	33.425	15.2
7	18.3	20.875	26.974999999999998	33.85
8	27.35	29.049999999999997	15.75	27.85
9	18.3	35.9	30.0	15.8
10	23.525	23.599999999999998	26.674999999999997	26.200000000000003
11	35.225	13.225000000000001	16.575	34.975
12	18.099999999999998	21.775	26.474999999999998	33.650000000000006
13	29.549999999999997	19.125	31.95	19.375
14	32.725	26.450000000000003	23.75	17.075000000000003
15	31.65	23.400000000000002	28.499999999999996	16.45
16	19.275000000000002	20.25	23.125	37.35
17	25.0	23.875	36.0	15.125
18	25.6	18.25	27.85	28.299999999999997
19	28.875	17.1	34.599999999999994	19.425
20	39.625	16.7	28.95	14.725
21	22.5	22.475	29.375	25.650000000000002
22	30.4	14.249999999999998	43.5	11.85
23	27.0	14.000000000000002	42.725	16.275000000000002
24	48.875	13.950000000000001	25.224999999999998	11.95
25	48.6	23.0	15.775	12.625
26	27.450000000000003	43.675000000000004	15.75	13.125
27	17.299999999999997	43.85	23.275000000000002	15.575
28	14.674999999999999	24.25	45.375	15.7
29	13.0	14.124999999999998	52.625	20.25
30	11.15	8.425	36.175000000000004	44.25
31	15.325	6.625	43.625	34.425
32	29.15	6.825	23.025000000000002	41.0
33	50.8	10.225	15.0	23.974999999999998
34	59.8	7.875	14.674999999999999	17.65
35	55.7	8.475000000000001	22.8	13.025
36	61.475	10.35	14.6	13.575000000000001
37	36.449999999999996	12.3	22.175	29.075
38	26.125	15.075	14.475	44.324999999999996
39	18.2	26.150000000000002	11.225	44.425
40	18.25	45.375	12.375	24.0
41	29.349999999999998	40.949999999999996	12.9	16.8
42	48.275	27.725	11.75	12.25
43	42.449999999999996	35.699999999999996	10.4	11.450000000000001
44	30.5	45.800000000000004	8.924999999999999	14.774999999999999
45	19.950000000000003	43.0	12.3	24.75
46	15.15	26.625	27.35	30.875000000000004
47	12.049999999999999	16.55	30.85	40.550000000000004
48	11.75	13.350000000000001	21.45	53.449999999999996
49	16.35	25.5	14.35	43.8
50	32.300000000000004	22.6	12.375	32.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	2.5
28	3.0
29	4.0
30	5.0
31	9.5
32	14.0
33	15.5
34	17.0
35	25.5
36	34.0
37	65.0
38	96.0
39	80.5
40	65.0
41	70.0
42	75.0
43	96.0
44	117.0
45	173.5
46	230.0
47	257.5
48	285.0
49	382.5
50	480.0
51	648.0
52	816.0
53	658.0
54	500.0
55	451.0
56	402.0
57	349.0
58	296.0
59	232.0
60	168.0
61	128.5
62	89.0
63	114.0
64	139.0
65	90.0
66	41.0
67	26.0
68	11.0
69	19.5
70	28.0
71	47.5
72	67.0
73	36.5
74	6.0
75	4.0
76	2.0
77	3.0
78	4.0
79	4.5
80	5.0
81	3.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22499999999999998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.0	44.074999999999996
2	7.348837209302325	7.9
3	3.1627906976744184	5.1
4	1.7674418604651163	3.8
5	1.2093023255813953	3.25
6	0.7441860465116279	2.4
7	0.46511627906976744	1.7500000000000002
8	0.6976744186046512	3.0
9	0.23255813953488372	1.125
>10	2.2325581395348837	20.825
>50	0.09302325581395349	3.85
>100	0.046511627906976744	2.9250000000000003
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	117	2.9250000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	100	2.5	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	54	1.35	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	39	0.975	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	31	0.775	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (96% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	22	0.5499999999999999	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC	19	0.475	RNA PCR Primer, Index 18 (100% over 50bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	19	0.475	RNA PCR Primer, Index 1 (97% over 35bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	18	0.44999999999999996	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	18	0.44999999999999996	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	17	0.42500000000000004	No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	16	0.4	RNA PCR Primer, Index 1 (96% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	15	0.375	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	15	0.375	RNA PCR Primer, Index 1 (96% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	14	0.35000000000000003	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	14	0.35000000000000003	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	13	0.325	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCG	13	0.325	RNA PCR Primer, Index 1 (96% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	13	0.325	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC	11	0.27499999999999997	No Hit
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	9	0.22499999999999998	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCG	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	8	0.2	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	8	0.2	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	8	0.2	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	8	0.2	RNA PCR Primer, Index 1 (96% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	8	0.2	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	8	0.2	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC	7	0.17500000000000002	RNA PCR Primer, Index 18 (100% over 44bp)
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	6	0.15	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC	6	0.15	RNA PCR Primer, Index 18 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	6	0.15	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGAAATTCCG	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAATTCCG	5	0.125	RNA PCR Primer, Index 1 (95% over 23bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CGCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	5	0.125	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	5	0.125	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	5	0.125	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.5	0.0	0.0	0.0
2	0.0	0.525	0.0	0.0	0.0
3	0.0	0.6	0.0	0.0	0.0
4	0.0	0.625	0.0	0.0	0.0
5	0.0	0.675	0.0	0.0	0.0
6	0.0	0.775	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.275	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.55	0.0	0.0	0.0
11	0.0	1.6	0.0	0.0	0.0
12	0.0	1.925	0.0	0.0	0.0
13	0.0	2.15	0.0	0.0	0.0
14	0.0	2.3	0.0	0.0	0.0
15	0.0	2.675	0.0	0.0	0.0
16	0.0	3.7	0.0	0.0	0.0
17	0.0	4.15	0.0	0.0	0.0
18	0.0	5.05	0.0	0.0	0.0
19	0.0	5.475	0.0	0.0	0.0
20	0.0	6.25	0.0	0.0	0.0
21	0.0	10.825	0.0	0.0	0.0
22	0.0	21.2	0.0	0.0	0.0
23	0.0	46.95	0.0	0.0	0.0
24	0.0	58.8	0.0	0.0	0.0
25	0.0	64.175	0.0	0.0	0.0
26	0.0	65.65	0.0	0.0	0.0
27	0.0	66.35	0.0	0.0	0.0
28	0.0	66.925	0.0	0.0	0.0
29	0.0	67.05	0.0	0.0	0.0
30	0.0	67.625	0.0	0.0	0.0
31	0.0	68.35	0.0	0.0	0.0
32	0.0	69.875	0.0	0.0	0.0
33	0.0	71.1	0.0	0.0	0.0
34	0.0	73.525	0.0	0.0	0.0
35	0.0	74.7	0.0	0.0	0.0
36	0.0	75.4	0.0	0.0	0.0
37	0.0	76.525	0.0	0.0	0.0
38	0.0	76.975	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCAGG	20	6.952051E-4	44.000004	36
ATGATGG	20	6.952051E-4	44.000004	20
ACATTCA	40	5.315087E-9	44.000004	2
CTGTTGG	20	6.952051E-4	44.000004	19
GTCGGTG	40	5.315087E-9	44.000004	14
AACATTC	40	5.315087E-9	44.000004	1
TGTCGGT	40	5.315087E-9	44.000004	13
ACAGTGA	35	1.02281774E-7	44.0	3
ACCGGTC	35	1.02281774E-7	44.0	10
AACGCTG	35	1.02281774E-7	44.0	8
GGTGAGT	35	1.02281774E-7	44.0	17
ACGCTGT	35	1.02281774E-7	44.0	9
AACCGGT	35	1.02281774E-7	44.0	9
GAGTGGA	25	3.6958358E-5	44.0	20
TTCAACG	35	1.02281774E-7	44.0	5
TCGGTGA	35	1.02281774E-7	44.0	15
GGTCTCT	35	1.02281774E-7	44.0	13
CTCTTTT	30	1.9515464E-6	44.0	16
AGTGGAA	25	3.6958358E-5	44.0	21
CATTCAA	35	1.02281774E-7	44.0	3
>>END_MODULE
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
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Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37588 spots for SRR5030831.sra
Written 37588 spots for SRR5030831.sra
Read 37635 spots for SRR5030831.sra
Written 37635 spots for SRR5030831.sra
SRR ids: ['SRR5030831.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_rnjhykkb
SRR5030831.sra spots: 1804271
blocks: [[1, 37588], [37589, 75176], [75177, 112764], [112765, 150352], [150353, 187940], [187941, 225528], [225529, 263116], [263117, 300704], [300705, 338292], [338293, 375880], [375881, 413468], [413469, 451056], [451057, 488644], [488645, 526232], [526233, 563820], [563821, 601408], [601409, 638996], [638997, 676584], [676585, 714172], [714173, 751760], [751761, 789348], [789349, 826936], [826937, 864524], [864525, 902112], [902113, 939700], [939701, 977288], [977289, 1014876], [1014877, 1052464], [1052465, 1090052], [1090053, 1127640], [1127641, 1165228], [1165229, 1202816], [1202817, 1240404], [1240405, 1277992], [1277993, 1315580], [1315581, 1353168], [1353169, 1390756], [1390757, 1428344], [1428345, 1465932], [1465933, 1503520], [1503521, 1541108], [1541109, 1578696], [1578697, 1616284], [1616285, 1653872], [1653873, 1691460], [1691461, 1729048], [1729049, 1766636], [1766637, 1804271]]
SRR5030831 file size 311640
SRR5030831 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR5030831 SRR5030831_1.fastq
Input file:	SRR5030831_1.fastq
trimmed:	SRR5030831-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Wed Mar  4 06:22:05 2020 >> started

Wed Mar  4 06:22:06 2020 >> done (1.231s)
1804271 reads processed; of these:
    152 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
1804113 (99.99%) reads available; of these:
 225352 (12.49%) trimmed reads available after processing
1578761 (87.51%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     38	  0.00%
 19	     52	  0.00%
 20	     77	  0.00%
 21	     96	  0.01%
 22	    181	  0.01%
 23	    261	  0.01%
 24	    247	  0.01%
 25	    379	  0.02%
 26	    348	  0.02%
 27	    446	  0.02%
 28	    519	  0.03%
 29	    786	  0.04%
 30	    735	  0.04%
 31	    931	  0.05%
 32	   2271	  0.13%
 33	   5972	  0.33%
 34	  13456	  0.75%
 35	   3521	  0.20%
 36	   3889	  0.22%
 37	   2192	  0.12%
 38	   4403	  0.24%
 39	   4349	  0.24%
 40	   8477	  0.47%
 41	   4562	  0.25%
 42	  12424	  0.69%
 43	   5358	  0.30%
 44	  20970	  1.16%
 45	  14359	  0.80%
 46	  13271	  0.74%
 47	  14468	  0.80%
 48	  39586	  2.19%
 49	  46728	  2.59%
 50	1578761	 87.51%
1804113 reads passed initial QC


criterion=sequence-density
sequence-density=75.96
sequence-density-rank=1
fanout-score=23.05
fanout-score-rank=3
prefix-density=78.60
prefix-fanout=22.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC


criterion=fanout-score
sequence-density=1.29
sequence-density-rank=6
fanout-score=584.41
fanout-score-rank=1
prefix-density=77.68
prefix-fanout=9.7
sequence=GGAATTCTCGGTGCCAAGGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC -o SRR5030831 -
Input file:	STDIN
trimmed:	SRR5030831-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Wed Mar  4 06:22:21 2020 >> started

Wed Mar  4 06:22:24 2020 >> done (2.585s)
1756636 reads processed; of these:
  77224 ( 4.40%) short reads filtered out after trimming by size control
  10903 ( 0.62%) empty reads filtered out after trimming by size control
1668509 (94.98%) reads available; of these:
1405299 (84.22%) trimmed reads available after processing
 263210 (15.78%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  10772	  0.65%
 19	  15733	  0.94%
 20	  65461	  3.92%
 21	 182993	 10.97%
 22	 437254	 26.21%
 23	 222031	 13.31%
 24	 105338	  6.31%
 25	  27468	  1.65%
 26	  14191	  0.85%
 27	  11363	  0.68%
 28	  11746	  0.70%
 29	  10604	  0.64%
 30	  14784	  0.89%
 31	  23933	  1.43%
 32	  18459	  1.11%
 33	  52589	  3.15%
 34	  21012	  1.26%
 35	  16602	  1.00%
 36	  25472	  1.53%
 37	   9045	  0.54%
 38	  10502	  0.63%
 39	   9481	  0.57%
 40	  14290	  0.86%
 41	  17377	  1.04%
 42	  22786	  1.37%
 43	  14919	  0.89%
 44	  14463	  0.87%
 45	  16628	  1.00%
 46	  13069	  0.78%
 47	  10470	  0.63%
 48	   4083	  0.24%
 49	   7337	  0.44%
 50	 216254	 12.96%


criterion=sequence-density
sequence-density=6.75
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=425.37
fanout-score-rank=1
prefix-density=3.01
prefix-fanout=1.6
sequence=TTGTATAGTTTAACAGTCTACAGCCATGGTCGTGGAATTCT
                                 Started job on |	Mar 04 06:23:03
                             Started mapping on |	Mar 04 06:23:04
                                    Finished on |	Mar 04 06:23:30
       Mapping speed, Million of reads per hour |	237.60

                          Number of input reads |	1715986
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	858562
                        Uniquely mapped reads % |	50.03%
                          Average mapped length |	27.98
                       Number of splices: Total |	1127
            Number of splices: Annotated (sjdb) |	238
                       Number of splices: GT/AG |	1045
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	64
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.31
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	575332
             % of reads mapped to multiple loci |	33.53%
        Number of reads mapped to too many loci |	40386
             % of reads mapped to too many loci |	2.35%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.57%
                     % of reads unmapped: other |	0.51%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	282092	282092	282092
N_multimapping	575332	575332	575332
N_noFeature	54204	64816	655457
N_ambiguous	441688	267160	8861
UnstrandedReadsAssigned:362670 PositiveStrandReadsAssigned:526586 NegativeStrandReadsAssigned:194244
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030831 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030831-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,715,986 reads, 360,491 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 837 rounds

 109283 SRR5030831.ke.tsv
  52636 SRR5030831.se.tsv
 161919 total
==> SRR5030831.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030831.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030831 completed mapping pipeline successfully
