Starting /dee2/code/volunteer_pipeline.sh SRR5030942
    current disk space = 160606617600
    free memory = 56711384 
SRR5030942 SRAfilesize
deccf02eb72b63f78296770827efa731  SRR5030942.sra
SRR5030942.sra file validated
SRR5030942 is single end
SRR5030942 is conventional basespace
SRR5030942 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3945	33.0	31.0	34.0	31.0	34.0
2	32.6605	34.0	31.0	34.0	31.0	34.0
3	32.83075	34.0	31.0	34.0	31.0	34.0
4	36.14	37.0	37.0	37.0	35.0	37.0
5	36.1585	37.0	37.0	37.0	35.0	37.0
6	36.06325	37.0	35.0	37.0	35.0	37.0
7	36.0095	37.0	35.0	37.0	35.0	37.0
8	36.0605	37.0	35.0	37.0	35.0	37.0
9	37.87175	39.0	38.0	39.0	35.0	39.0
10	37.92825	39.0	38.0	39.0	35.0	39.0
11	37.771	39.0	38.0	39.0	35.0	39.0
12	37.792	39.0	38.0	39.0	35.0	39.0
13	37.76775	39.0	38.0	39.0	35.0	39.0
14	39.104	41.0	39.0	41.0	35.0	41.0
15	39.15625	41.0	39.0	41.0	36.0	41.0
16	39.175	40.0	38.0	41.0	36.0	41.0
17	39.25075	41.0	39.0	41.0	36.0	41.0
18	39.23925	41.0	39.0	41.0	36.0	41.0
19	38.99325	40.0	39.0	41.0	35.0	41.0
20	39.0215	40.0	39.0	41.0	35.0	41.0
21	39.10525	40.0	39.0	41.0	36.0	41.0
22	38.96	40.0	39.0	41.0	35.0	41.0
23	38.9065	40.0	38.0	41.0	35.0	41.0
24	38.58925	40.0	38.0	41.0	34.0	41.0
25	38.87275	40.0	39.0	41.0	35.0	41.0
26	38.9225	40.0	38.0	41.0	35.0	41.0
27	38.93475	40.0	39.0	41.0	35.0	41.0
28	38.5765	40.0	38.0	41.0	34.0	41.0
29	37.89925	40.0	38.0	41.0	32.0	41.0
30	38.17675	40.0	38.0	41.0	33.0	41.0
31	38.4785	40.0	38.0	41.0	34.0	41.0
32	38.6935	40.0	38.0	41.0	34.0	41.0
33	37.9445	40.0	38.0	41.0	33.0	41.0
34	37.8915	40.0	37.0	41.0	33.0	41.0
35	37.59425	39.0	37.0	40.0	33.0	41.0
36	34.4635	38.0	32.0	40.0	23.0	41.0
37	35.5085	38.0	34.0	40.0	27.0	41.0
38	36.913	39.0	36.0	40.0	31.0	41.0
39	36.80125	39.0	36.0	40.0	31.0	41.0
40	36.8415	39.0	36.0	40.0	31.0	41.0
41	36.88275	39.0	36.0	40.0	31.0	41.0
42	37.114	40.0	37.0	41.0	32.0	41.0
43	36.85175	39.0	36.0	41.0	31.0	41.0
44	36.7465	39.0	36.0	41.0	30.0	41.0
45	36.42275	39.0	36.0	40.0	30.0	41.0
46	34.46825	38.0	33.0	40.0	24.0	41.0
47	35.14125	38.0	34.0	40.0	27.0	41.0
48	35.4495	39.0	35.0	40.0	28.0	41.0
49	34.298	38.0	34.0	40.0	24.0	41.0
50	29.6775	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	1.0
19	3.0
20	6.0
21	6.0
22	10.0
23	9.0
24	14.0
25	15.0
26	25.0
27	35.0
28	27.0
29	48.0
30	39.0
31	73.0
32	102.0
33	133.0
34	179.0
35	204.0
36	303.0
37	527.0
38	1001.0
39	1236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.533650237678259	23.592694520890667	50.16262196647485	14.711033274956216
2	21.9	28.025	19.625	30.45
3	27.05	16.925	21.6	34.425
4	33.525	28.849999999999998	13.55	24.075
5	21.0	40.975	23.025000000000002	15.0
6	28.749999999999996	17.075000000000003	40.45	13.725000000000001
7	20.45	18.35	22.35	38.85
8	21.05	32.175	15.35	31.424999999999997
9	25.424999999999997	32.6	27.675	14.299999999999999
10	24.325	17.925	32.4	25.35
11	29.725	16.0	15.125	39.15
12	13.825000000000001	21.175	34.449999999999996	30.55
13	28.725	19.725	29.025000000000002	22.525000000000002
14	26.325	33.324999999999996	21.55	18.8
15	36.68417104276069	23.48087021755439	23.78094523630908	16.054013503375845
16	17.075000000000003	25.374999999999996	19.7	37.85
17	23.625	22.525000000000002	39.275	14.575
18	22.575	18.675	36.05	22.7
19	36.675000000000004	14.799999999999999	29.725	18.8
20	44.074999999999996	15.75	25.7	14.475
21	24.125	19.675	24.75	31.45
22	28.375	15.1	45.45	11.075
23	25.75	13.325000000000001	44.2	16.725
24	48.6	16.275000000000002	24.7	10.424999999999999
25	49.025	23.5	14.6	12.875
26	26.724999999999998	45.125	16.225	11.924999999999999
27	17.875	46.45	22.625	13.05
28	13.8	25.1	45.225	15.875
29	10.125	14.875	56.45	18.55
30	10.7	8.3	34.050000000000004	46.949999999999996
31	15.525	6.875000000000001	46.150000000000006	31.45
32	27.775	7.55	21.65	43.025000000000006
33	53.825	9.9	14.05	22.225
34	58.45	8.75	17.2	15.6
35	61.85000000000001	9.625	17.025000000000002	11.5
36	40.65	10.45	33.900000000000006	15.0
37	44.824999999999996	11.125	20.200000000000003	23.849999999999998
38	22.775000000000002	16.175	14.075	46.975
39	17.224999999999998	23.25	10.25	49.275000000000006
40	16.6	45.875	12.975	24.55
41	24.4	47.099999999999994	10.05	18.45
42	49.45	30.0	10.549999999999999	10.0
43	48.6	31.15	9.575	10.674999999999999
44	29.75	47.275	8.825	14.149999999999999
45	19.175	50.1	14.124999999999998	16.6
46	13.175	28.075	17.925	40.825
47	8.774999999999999	16.950000000000003	38.65	35.625
48	11.225	14.05	18.9	55.825
49	15.299999999999999	20.075000000000003	14.424999999999999	50.2
50	18.5	37.4	13.525	30.575000000000003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	1.5
28	1.0
29	4.5
30	8.0
31	15.5
32	23.0
33	23.0
34	23.0
35	36.0
36	49.0
37	47.5
38	46.0
39	52.0
40	58.0
41	55.5
42	53.0
43	101.0
44	149.0
45	179.0
46	209.0
47	330.5
48	452.0
49	510.0
50	568.0
51	656.0
52	744.0
53	565.0
54	386.0
55	468.0
56	550.0
57	437.5
58	325.0
59	210.0
60	95.0
61	70.0
62	45.0
63	77.5
64	110.0
65	78.0
66	46.0
67	30.5
68	15.0
69	11.5
70	8.0
71	14.0
72	20.0
73	11.5
74	3.0
75	3.0
76	3.0
77	3.5
78	4.0
79	3.5
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.849999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09179926560587	30.675
2	9.8531211750306	8.05
3	3.9167686658506726	4.8
4	2.692778457772338	4.3999999999999995
5	1.8359853121175032	3.75
6	0.7343941248470013	1.7999999999999998
7	1.2239902080783354	3.5000000000000004
8	0.6731946144430845	2.1999999999999997
9	0.30599755201958384	1.125
>10	3.243574051407589	23.225
>50	0.30599755201958384	10.274999999999999
>100	0.12239902080783352	6.2
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	145	3.6249999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	94	2.35	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	91	2.275	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	87	2.175	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	45	1.125	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	31	0.775	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC	24	0.6	RNA PCR Primer, Index 30 (100% over 44bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	24	0.6	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	23	0.575	RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	20	0.5	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	17	0.42500000000000004	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	17	0.42500000000000004	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	16	0.4	RNA PCR Primer, Index 7 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGC	15	0.375	RNA PCR Primer, Index 30 (100% over 50bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	15	0.375	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	13	0.325	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	13	0.325	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	12	0.3	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	8	0.2	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	8	0.2	RNA PCR Primer, Index 1 (100% over 32bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	8	0.2	No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	8	0.2	RNA PCR Primer, Index 7 (100% over 35bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	7	0.17500000000000002	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	7	0.17500000000000002	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCTTGGAATT	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTAT	5	0.125	RNA PCR Primer, Index 30 (100% over 48bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	5	0.125	RNA PCR Primer, Index 1 (96% over 29bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	5	0.125	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.4	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.525	0.0	0.0	0.0
5	0.0	0.575	0.0	0.0	0.0
6	0.0	0.675	0.0	0.0	0.0
7	0.0	1.375	0.0	0.0	0.0
8	0.0	1.675	0.0	0.0	0.0
9	0.0	1.775	0.0	0.0	0.0
10	0.0	1.9	0.0	0.0	0.0
11	0.0	2.125	0.0	0.0	0.0
12	0.0	2.475	0.0	0.0	0.0
13	0.0	2.8	0.0	0.0	0.0
14	0.0	3.075	0.0	0.0	0.0
15	0.0	3.5	0.0	0.0	0.0
16	0.0	4.6	0.0	0.0	0.0
17	0.0	5.0	0.0	0.0	0.0
18	0.0	6.0	0.0	0.0	0.0
19	0.0	6.625	0.0	0.0	0.0
20	0.0	7.475	0.0	0.0	0.0
21	0.0	13.35	0.0	0.0	0.0
22	0.0	23.2	0.0	0.0	0.0
23	0.0	51.6	0.0	0.0	0.0
24	0.0	62.95	0.0	0.0	0.0
25	0.0	68.95	0.0	0.0	0.0
26	0.0	70.525	0.0	0.0	0.0
27	0.0	71.125	0.0	0.0	0.0
28	0.0	71.55	0.0	0.0	0.0
29	0.0	72.0	0.0	0.0	0.0
30	0.0	72.375	0.0	0.0	0.0
31	0.0	73.1	0.0	0.0	0.0
32	0.0	74.85	0.0	0.0	0.0
33	0.0	75.525	0.0	0.0	0.0
34	0.0	77.725	0.0	0.0	0.0
35	0.0	78.4	0.0	0.0	0.0
36	0.0	78.95	0.0	0.0	0.0
37	0.0	79.65	0.0	0.0	0.0
38	0.0	80.325	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
ACGCTGT	20	6.952051E-4	44.000004	9
TAAACAT	20	6.952051E-4	44.000004	3
AAACATC	20	6.952051E-4	44.000004	4
TTCAACG	20	6.952051E-4	44.000004	5
CATCCCC	20	6.952051E-4	44.000004	7
TCAACGC	20	6.952051E-4	44.000004	6
ACATCCC	20	6.952051E-4	44.000004	6
CCCCGAC	20	6.952051E-4	44.000004	10
GAACTGT	20	6.952051E-4	44.000004	16
GACTGGA	20	6.952051E-4	44.000004	14
GTGATGA	20	6.952051E-4	44.000004	6
TTGGCTT	20	6.952051E-4	44.000004	17
CGACTGG	20	6.952051E-4	44.000004	13
TGGCTTG	20	6.952051E-4	44.000004	18
TGTAAAC	20	6.952051E-4	44.000004	1
GTAAACA	20	6.952051E-4	44.000004	2
AACATCC	20	6.952051E-4	44.000004	5
CAACGCT	20	6.952051E-4	44.000004	7
CGCTGTC	20	6.952051E-4	44.000004	10
>>END_MODULE
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217797 spots for SRR5030942.sra
Written 217797 spots for SRR5030942.sra
Read 217799 spots for SRR5030942.sra
Written 217799 spots for SRR5030942.sra
SRR ids: ['SRR5030942.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_oamsd3ce
SRR5030942.sra spots: 2613566
blocks: [[1, 217797], [217798, 435594], [435595, 653391], [653392, 871188], [871189, 1088985], [1088986, 1306782], [1306783, 1524579], [1524580, 1742376], [1742377, 1960173], [1960174, 2177970], [2177971, 2395767], [2395768, 2613566]]
SRR5030942 file size 451909
SRR5030942 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030942 SRR5030942_1.fastq
Input file:	SRR5030942_1.fastq
trimmed:	SRR5030942-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:50:28 2020 >> started

Wed Mar  4 06:50:29 2020 >> done (1.747s)
2613566 reads processed; of these:
    162 ( 0.01%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
2613396 (99.99%) reads available; of these:
 167568 ( 6.41%) trimmed reads available after processing
2445828 (93.59%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     43	  0.00%
 19	     62	  0.00%
 20	    130	  0.00%
 21	    159	  0.01%
 22	    264	  0.01%
 23	    303	  0.01%
 24	    336	  0.01%
 25	    415	  0.02%
 26	    354	  0.01%
 27	    498	  0.02%
 28	    618	  0.02%
 29	   1030	  0.04%
 30	    645	  0.02%
 31	    906	  0.03%
 32	    733	  0.03%
 33	   1097	  0.04%
 34	   2345	  0.09%
 35	   2142	  0.08%
 36	   1636	  0.06%
 37	   1880	  0.07%
 38	   2095	  0.08%
 39	   2495	  0.10%
 40	   2727	  0.10%
 41	   3060	  0.12%
 42	   3909	  0.15%
 43	   5550	  0.21%
 44	   9951	  0.38%
 45	  12740	  0.49%
 46	  11714	  0.45%
 47	  16876	  0.65%
 48	  34372	  1.32%
 49	  46483	  1.78%
 50	2445828	 93.59%
2613396 reads passed initial QC


criterion=sequence-density
sequence-density=80.33
sequence-density-rank=1
fanout-score=18.83
fanout-score-rank=1
prefix-density=82.22
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC


criterion=fanout-score
sequence-density=80.33
sequence-density-rank=1
fanout-score=18.83
fanout-score-rank=1
prefix-density=82.22
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC -o SRR5030942 -
Input file:	STDIN
trimmed:	SRR5030942-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:50:43 2020 >> started

Wed Mar  4 06:50:48 2020 >> done (4.168s)
2548868 reads processed; of these:
 152588 ( 5.99%) short reads filtered out after trimming by size control
  10011 ( 0.39%) empty reads filtered out after trimming by size control
2386269 (93.62%) reads available; of these:
2108104 (88.34%) trimmed reads available after processing
 278165 (11.66%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15509	  0.65%
 19	  20379	  0.85%
 20	 139983	  5.87%
 21	 273080	 11.44%
 22	 739953	 31.01%
 23	 294816	 12.35%
 24	 148849	  6.24%
 25	  40746	  1.71%
 26	  18820	  0.79%
 27	  14616	  0.61%
 28	  16071	  0.67%
 29	  11954	  0.50%
 30	  18125	  0.76%
 31	  34147	  1.43%
 32	  22216	  0.93%
 33	  54417	  2.28%
 34	  20434	  0.86%
 35	  16261	  0.68%
 36	  16610	  0.70%
 37	  12108	  0.51%
 38	  12896	  0.54%
 39	  13801	  0.58%
 40	  20967	  0.88%
 41	  25973	  1.09%
 42	  30429	  1.28%
 43	  21840	  0.92%
 44	  17335	  0.73%
 45	  23695	  0.99%
 46	  17312	  0.73%
 47	  13004	  0.54%
 48	   3134	  0.13%
 49	  10816	  0.45%
 50	 245973	 10.31%


criterion=sequence-density
sequence-density=5.65
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.51
sequence-density-rank=29
fanout-score=19.56
fanout-score-rank=1
prefix-density=9.89
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:51:08
                             Started mapping on |	Mar 04 06:51:09
                                    Finished on |	Mar 04 06:51:32
       Mapping speed, Million of reads per hour |	383.60

                          Number of input reads |	2450797
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1517664
                        Uniquely mapped reads % |	61.93%
                          Average mapped length |	27.24
                       Number of splices: Total |	1127
            Number of splices: Annotated (sjdb) |	238
                       Number of splices: GT/AG |	1034
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	74
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.58
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	669317
             % of reads mapped to multiple loci |	27.31%
        Number of reads mapped to too many loci |	49049
             % of reads mapped to too many loci |	2.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.90%
                     % of reads unmapped: other |	0.86%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	263816	263816	263816
N_multimapping	669317	669317	669317
N_noFeature	72380	89467	1030570
N_ambiguous	835777	409951	12817
UnstrandedReadsAssigned:609507 PositiveStrandReadsAssigned:1018246 NegativeStrandReadsAssigned:474277
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030942 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030942-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,450,797 reads, 665,425 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,057 rounds

 109283 SRR5030942.ke.tsv
  52636 SRR5030942.se.tsv
 161919 total
==> SRR5030942.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030942.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030942 completed mapping pipeline successfully
