Starting /dee2/code/volunteer_pipeline.sh SRR5031093
    current disk space = 160489226240
    free memory = 73954960 
SRR5031093 SRAfilesize
a6ad479acab95561c26930d89dc4885b  SRR5031093.sra
SRR5031093.sra file validated
SRR5031093 is single end
SRR5031093 is conventional basespace
SRR5031093 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88975	31.0	31.0	34.0	30.0	34.0
2	32.428	33.0	31.0	34.0	31.0	34.0
3	32.458	34.0	31.0	34.0	31.0	34.0
4	36.1575	37.0	35.0	37.0	35.0	37.0
5	36.125	37.0	35.0	37.0	35.0	37.0
6	36.0575	37.0	35.0	37.0	35.0	37.0
7	35.84125	37.0	35.0	37.0	35.0	37.0
8	35.681	37.0	35.0	37.0	33.0	37.0
9	36.9255	39.0	37.0	39.0	33.0	39.0
10	36.529	39.0	35.0	39.0	32.0	39.0
11	37.424	39.0	37.0	39.0	34.0	39.0
12	37.68525	39.0	37.0	39.0	35.0	39.0
13	37.441	39.0	37.0	39.0	35.0	39.0
14	38.2255	40.0	38.0	41.0	33.0	41.0
15	36.9555	40.0	36.0	41.0	30.0	41.0
16	36.8005	39.0	36.0	40.0	31.0	41.0
17	36.61475	39.0	36.0	40.0	30.0	41.0
18	38.11225	39.0	37.0	41.0	34.0	41.0
19	38.56975	40.0	38.0	41.0	35.0	41.0
20	38.28975	40.0	38.0	41.0	34.0	41.0
21	38.576	40.0	38.0	41.0	35.0	41.0
22	37.34725	39.0	37.0	40.0	32.0	41.0
23	37.55725	39.0	37.0	40.0	32.0	41.0
24	36.958	39.0	36.0	40.0	30.0	41.0
25	38.00125	40.0	37.0	41.0	33.0	41.0
26	37.95675	40.0	38.0	41.0	33.0	41.0
27	38.19375	40.0	38.0	41.0	34.0	41.0
28	38.42025	40.0	38.0	41.0	34.0	41.0
29	38.357	40.0	38.0	41.0	34.0	41.0
30	38.71325	40.0	38.0	41.0	35.0	41.0
31	38.74	40.0	38.0	41.0	35.0	41.0
32	38.7545	40.0	38.0	41.0	35.0	41.0
33	38.6975	40.0	38.0	41.0	35.0	41.0
34	38.0275	40.0	38.0	41.0	33.0	41.0
35	37.3925	40.0	36.0	41.0	30.0	41.0
36	33.43325	35.0	30.0	40.0	23.0	41.0
37	35.0895	37.0	33.0	39.0	30.0	40.0
38	36.2655	38.0	35.0	40.0	30.0	40.0
39	36.665	38.0	36.0	40.0	31.0	40.0
40	36.88125	39.0	36.0	40.0	31.0	41.0
41	37.1735	39.0	37.0	40.0	31.0	41.0
42	37.14975	40.0	37.0	40.0	31.0	41.0
43	37.39375	40.0	37.0	41.0	33.0	41.0
44	36.455	39.0	36.0	40.0	30.0	41.0
45	36.51175	39.0	36.0	40.0	30.0	41.0
46	36.54575	39.0	36.0	40.0	30.0	41.0
47	35.4595	38.0	34.0	40.0	27.0	41.0
48	36.5525	39.0	36.0	40.0	30.0	41.0
49	36.786	40.0	36.0	41.0	31.0	41.0
50	34.794	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	1.0
18	2.0
19	2.0
20	8.0
21	4.0
22	5.0
23	7.0
24	7.0
25	21.0
26	11.0
27	27.0
28	26.0
29	35.0
30	42.0
31	68.0
32	98.0
33	132.0
34	186.0
35	280.0
36	472.0
37	786.0
38	1142.0
39	635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.5048786589942456	26.920190142606952	50.58794095571679	15.986990242682012
2	24.637318659329665	28.414207103551774	15.482741370685343	31.465732866433214
3	26.125	18.2	18.975	36.7
4	34.599999999999994	29.25	12.975	23.175
5	22.3	38.35	29.549999999999997	9.8
6	30.525000000000002	13.200000000000001	42.0	14.274999999999999
7	13.175	23.849999999999998	20.275000000000002	42.699999999999996
8	24.6	28.775000000000002	14.85	31.775
9	23.400000000000002	33.125	29.049999999999997	14.424999999999999
10	18.425	19.075	29.725	32.775
11	36.55	8.95	14.025000000000002	40.475
12	16.75	15.775	31.25	36.225
13	31.65	14.099999999999998	36.425000000000004	17.825
14	30.575000000000003	30.625000000000004	21.4	17.4
15	35.65	18.325	28.599999999999998	17.424999999999997
16	18.45	16.55	21.275	43.725
17	28.95	19.650000000000002	35.9	15.5
18	26.674999999999997	15.075	31.724999999999998	26.525
19	38.65	14.625	29.4	17.325
20	49.225	12.875	25.724999999999998	12.174999999999999
21	24.075	21.55	26.55	27.825
22	32.1	10.15	45.9	11.85
23	26.35	12.7	46.825	14.124999999999998
24	50.05	11.125	27.825	11.0
25	51.300000000000004	24.425	15.8	8.475000000000001
26	28.875	44.95	14.000000000000002	12.174999999999999
27	16.725	48.125	22.45	12.7
28	11.275	26.974999999999998	46.975	14.774999999999999
29	12.9	12.1	57.25	17.75
30	8.975	6.8500000000000005	38.725	45.45
31	14.399999999999999	4.75	47.125	33.725
32	28.425	5.45	23.075000000000003	43.05
33	52.7	9.0	13.175	25.124999999999996
34	62.925	6.75	16.425	13.900000000000002
35	67.425	6.05	17.775	8.75
36	46.125	7.3	33.875	12.7
37	47.55	9.975000000000001	20.125	22.35
38	24.625	17.599999999999998	11.35	46.425
39	17.224999999999998	23.200000000000003	9.55	50.025
40	15.225	48.025	10.100000000000001	26.650000000000002
41	24.275	49.275000000000006	8.7	17.75
42	46.550000000000004	32.2	10.45	10.8
43	52.0	30.225	7.049999999999999	10.725
44	32.45	47.625	6.225	13.700000000000001
45	20.525	51.300000000000004	11.075	17.1
46	13.325000000000001	27.85	16.725	42.1
47	7.775	17.150000000000002	37.3	37.775
48	10.2	14.374999999999998	21.15	54.275
49	14.099999999999998	17.825	13.575000000000001	54.50000000000001
50	17.375	35.425000000000004	13.5	33.7
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	0.5
26	0.0
27	0.0
28	0.0
29	1.5
30	3.0
31	4.5
32	6.0
33	6.0
34	6.0
35	10.0
36	14.0
37	22.5
38	31.0
39	26.5
40	22.0
41	24.5
42	27.0
43	53.5
44	80.0
45	110.5
46	141.0
47	238.0
48	335.0
49	417.5
50	500.0
51	739.0
52	978.0
53	745.0
54	512.0
55	562.5
56	613.0
57	460.5
58	308.0
59	209.5
60	111.0
61	87.5
62	64.0
63	89.0
64	114.0
65	83.0
66	52.0
67	37.5
68	23.0
69	21.5
70	20.0
71	26.0
72	32.0
73	19.5
74	7.0
75	3.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97513321492006	32.925
2	9.650680876258141	8.15
3	3.315571343990527	4.2
4	1.7761989342806392	3.0
5	1.5985790408525755	3.375
6	0.8288928359976317	2.1
7	0.8880994671403196	2.625
8	0.4736530491415038	1.6
9	0.8880994671403196	3.375
>10	2.1314387211367674	18.4
>50	0.2960331557134399	9.700000000000001
>100	0.17761989342806395	10.549999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	175	4.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	144	3.5999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	90	2.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	88	2.1999999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	78	1.95	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	72	1.7999999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	60	1.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	46	1.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC	36	0.8999999999999999	RNA PCR Primer, Index 17 (100% over 50bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	33	0.8250000000000001	RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	23	0.575	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (96% over 30bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	19	0.475	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	18	0.44999999999999996	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	11	0.27499999999999997	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	9	0.22499999999999998	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTACCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACGTAGAGATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 17 (98% over 50bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGACTCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	8	0.2	RNA PCR Primer, Index 6 (100% over 34bp)
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	7	0.17500000000000002	RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	7	0.17500000000000002	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	7	0.17500000000000002	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA	6	0.15	RNA PCR Primer, Index 3 (97% over 36bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGCCGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	6	0.15	No Hit
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGACTTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (96% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	5	0.125	No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGCCTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTC	5	0.125	RNA PCR Primer, Index 17 (100% over 44bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.5	0.0	0.0	0.0
2	0.0	1.5	0.0	0.0	0.0
3	0.0	1.7	0.0	0.0	0.0
4	0.0	1.75	0.0	0.0	0.0
5	0.0	1.8	0.0	0.0	0.0
6	0.0	1.85	0.0	0.0	0.0
7	0.0	2.15	0.0	0.0	0.0
8	0.0	2.375	0.0	0.0	0.0
9	0.0	2.425	0.0	0.0	0.0
10	0.0	2.575	0.0	0.0	0.0
11	0.0	2.75	0.0	0.0	0.0
12	0.0	2.9	0.0	0.0	0.0
13	0.0	3.1	0.0	0.0	0.0
14	0.0	3.225	0.0	0.0	0.0
15	0.0	3.775	0.0	0.0	0.0
16	0.0	5.075	0.0	0.0	0.0
17	0.0	5.95	0.0	0.0	0.0
18	0.0	6.875	0.0	0.0	0.0
19	0.0	7.3	0.0	0.0	0.0
20	0.0	8.225	0.0	0.0	0.0
21	0.0	13.175	0.0	0.0	0.0
22	0.0	23.725	0.0	0.0	0.0
23	0.0	52.725	0.0	0.0	0.0
24	0.0	67.375	0.0	0.0	0.0
25	0.0	73.625	0.0	0.0	0.0
26	0.0	75.525	0.0	0.0	0.0
27	0.0	76.6	0.0	0.0	0.0
28	0.0	77.3	0.0	0.0	0.0
29	0.0	77.9	0.0	0.0	0.0
30	0.0	78.425	0.0	0.0	0.0
31	0.0	79.25	0.0	0.0	0.0
32	0.0	79.9	0.0	0.0	0.0
33	0.0	80.65	0.0	0.0	0.0
34	0.0	84.05	0.0	0.0	0.0
35	0.0	84.925	0.0	0.0	0.0
36	0.0	85.725	0.0	0.0	0.0
37	0.0	86.475	0.0	0.0	0.0
38	0.0	86.725	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTTG	20	6.952051E-4	44.000004	18
GTTGAAG	20	6.952051E-4	44.000004	10
AACTGTT	20	6.952051E-4	44.000004	17
AGTAGGT	20	6.952051E-4	44.000004	7
AACCTGT	20	6.952051E-4	44.000004	16
GAAGAAC	20	6.952051E-4	44.000004	13
AGAACTG	20	6.952051E-4	44.000004	15
GTGAGTT	20	6.952051E-4	44.000004	18
TAGTAGG	20	6.952051E-4	44.000004	6
GAACTGT	20	6.952051E-4	44.000004	16
TGAAGAA	20	6.952051E-4	44.000004	12
AAGAACT	20	6.952051E-4	44.000004	14
AGGTTGT	20	6.952051E-4	44.000004	10
AGTTGAA	20	6.952051E-4	44.000004	9
TTGGCTT	20	6.952051E-4	44.000004	17
GTAGGTT	20	6.952051E-4	44.000004	8
TTGAAGA	20	6.952051E-4	44.000004	11
TAGGTTG	20	6.952051E-4	44.000004	9
TGAGACC	35	1.02281774E-7	44.0	5
AACGCTG	30	1.9515464E-6	44.0	8
>>END_MODULE
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
Read 136745 spots for SRR5031093.sra
Written 136745 spots for SRR5031093.sra
SRR ids: ['SRR5031093.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_lpmrgnnh
SRR5031093.sra spots: 1640940
blocks: [[1, 136745], [136746, 273490], [273491, 410235], [410236, 546980], [546981, 683725], [683726, 820470], [820471, 957215], [957216, 1093960], [1093961, 1230705], [1230706, 1367450], [1367451, 1504195], [1504196, 1640940]]
SRR5031093 file size 283339
SRR5031093 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031093 SRR5031093_1.fastq
Input file:	SRR5031093_1.fastq
trimmed:	SRR5031093-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:28 2020 >> started

Wed Mar  4 07:22:30 2020 >> done (2.196s)
1640940 reads processed; of these:
    129 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
1640805 (99.99%) reads available; of these:
 140517 ( 8.56%) trimmed reads available after processing
1500288 (91.44%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     36	  0.00%
 19	     71	  0.00%
 20	    127	  0.01%
 21	    163	  0.01%
 22	    266	  0.02%
 23	    288	  0.02%
 24	    371	  0.02%
 25	    528	  0.03%
 26	    334	  0.02%
 27	    418	  0.03%
 28	    520	  0.03%
 29	    773	  0.05%
 30	    546	  0.03%
 31	    582	  0.04%
 32	    863	  0.05%
 33	   1463	  0.09%
 34	   2018	  0.12%
 35	   4853	  0.30%
 36	   2915	  0.18%
 37	   2797	  0.17%
 38	   2650	  0.16%
 39	   2942	  0.18%
 40	   3364	  0.21%
 41	   3411	  0.21%
 42	   4000	  0.24%
 43	   6478	  0.39%
 44	   7321	  0.45%
 45	  10142	  0.62%
 46	  14137	  0.86%
 47	   9926	  0.60%
 48	  26315	  1.60%
 49	  29899	  1.82%
 50	1500288	 91.44%
1640805 reads passed initial QC


criterion=sequence-density
sequence-density=87.13
sequence-density-rank=1
fanout-score=19.77
fanout-score-rank=2
prefix-density=87.62
prefix-fanout=19.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC


criterion=fanout-score
sequence-density=3.69
sequence-density-rank=2
fanout-score=71.04
fanout-score-rank=1
prefix-density=87.32
prefix-fanout=3.0
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC -o SRR5031093 -
Input file:	STDIN
trimmed:	SRR5031093-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:47 2020 >> started

Wed Mar  4 07:22:52 2020 >> done (4.365s)
1603514 reads processed; of these:
  97245 ( 6.06%) short reads filtered out after trimming by size control
  24292 ( 1.51%) empty reads filtered out after trimming by size control
1481977 (92.42%) reads available; of these:
1416849 (95.61%) trimmed reads available after processing
  65128 ( 4.39%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   9228	  0.62%
 19	  17395	  1.17%
 20	  87342	  5.89%
 21	 180337	 12.17%
 22	 498748	 33.65%
 23	 242892	 16.39%
 24	 101038	  6.82%
 25	  29602	  2.00%
 26	  14840	  1.00%
 27	  10857	  0.73%
 28	  11629	  0.78%
 29	   9432	  0.64%
 30	  11795	  0.80%
 31	  13288	  0.90%
 32	  14019	  0.95%
 33	  61139	  4.13%
 34	  13610	  0.92%
 35	  10625	  0.72%
 36	  12457	  0.84%
 37	   5867	  0.40%
 38	   6438	  0.43%
 39	   5888	  0.40%
 40	   8639	  0.58%
 41	  10291	  0.69%
 42	  10967	  0.74%
 43	   7528	  0.51%
 44	   5288	  0.36%
 45	   6860	  0.46%
 46	   4926	  0.33%
 47	   3745	  0.25%
 48	    778	  0.05%
 49	   1198	  0.08%
 50	  53291	  3.60%


criterion=sequence-density
sequence-density=9.67
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=0.45
sequence-density-rank=26
fanout-score=29.70
fanout-score-rank=1
prefix-density=13.15
prefix-fanout=1.0
sequence=CTGAGCTTGGCA
                                 Started job on |	Mar 04 07:24:06
                             Started mapping on |	Mar 04 07:24:07
                                    Finished on |	Mar 04 07:24:32
       Mapping speed, Million of reads per hour |	218.77

                          Number of input reads |	1519268
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	788425
                        Uniquely mapped reads % |	51.90%
                          Average mapped length |	23.93
                       Number of splices: Total |	3763
            Number of splices: Annotated (sjdb) |	1120
                       Number of splices: GT/AG |	3703
                       Number of splices: GC/AG |	32
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	28
                      Mismatch rate per base, % |	0.38%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.18
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	539740
             % of reads mapped to multiple loci |	35.53%
        Number of reads mapped to too many loci |	21944
             % of reads mapped to too many loci |	1.44%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.56%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	191103	191103	191103
N_multimapping	539740	539740	539740
N_noFeature	44934	53562	506679
N_ambiguous	462775	216427	5943
UnstrandedReadsAssigned:280716 PositiveStrandReadsAssigned:518436 NegativeStrandReadsAssigned:275803
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031093 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031093-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,519,268 reads, 397,493 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,036 rounds

 109283 SRR5031093.ke.tsv
  52636 SRR5031093.se.tsv
 161919 total
==> SRR5031093.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031093.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031093 completed mapping pipeline successfully
