Starting /dee2/code/volunteer_pipeline.sh SRR5030839
    current disk space = 160661749760
    free memory = 73970040 
SRR5030839 SRAfilesize
230d210bfdb2f96ec5111c8a6dc3f468  SRR5030839.sra
SRR5030839.sra file validated
SRR5030839 is single end
SRR5030839 is conventional basespace
SRR5030839 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030839_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.538	34.0	31.0	34.0	31.0	34.0
2	32.82425	34.0	31.0	34.0	31.0	34.0
3	32.9145	34.0	31.0	34.0	31.0	34.0
4	36.248	37.0	37.0	37.0	35.0	37.0
5	36.31525	37.0	37.0	37.0	35.0	37.0
6	36.18	37.0	37.0	37.0	35.0	37.0
7	36.1285	37.0	35.0	37.0	35.0	37.0
8	36.25625	37.0	36.0	37.0	35.0	37.0
9	38.054	39.0	39.0	39.0	35.0	39.0
10	38.068	39.0	39.0	39.0	35.0	39.0
11	37.9125	39.0	38.0	39.0	35.0	39.0
12	37.8835	39.0	38.0	39.0	35.0	39.0
13	37.8965	39.0	38.0	39.0	35.0	39.0
14	39.3705	41.0	39.0	41.0	36.0	41.0
15	39.23725	41.0	39.0	41.0	36.0	41.0
16	39.227	40.0	39.0	41.0	36.0	41.0
17	39.45725	41.0	39.0	41.0	37.0	41.0
18	39.445	41.0	39.0	41.0	36.0	41.0
19	39.2705	41.0	39.0	41.0	36.0	41.0
20	39.24725	41.0	39.0	41.0	36.0	41.0
21	39.3345	41.0	39.0	41.0	36.0	41.0
22	39.27075	41.0	39.0	41.0	36.0	41.0
23	39.1455	41.0	39.0	41.0	36.0	41.0
24	38.976	40.0	39.0	41.0	35.0	41.0
25	39.1795	41.0	39.0	41.0	36.0	41.0
26	39.32475	41.0	39.0	41.0	36.0	41.0
27	39.3315	41.0	39.0	41.0	36.0	41.0
28	39.1185	41.0	39.0	41.0	36.0	41.0
29	38.2495	40.0	38.0	41.0	33.0	41.0
30	38.59675	40.0	38.0	41.0	34.0	41.0
31	38.804	40.0	38.0	41.0	35.0	41.0
32	39.05575	41.0	39.0	41.0	35.0	41.0
33	38.1175	40.0	38.0	41.0	33.0	41.0
34	38.102	40.0	38.0	41.0	33.0	41.0
35	37.86625	40.0	37.0	41.0	33.0	41.0
36	34.45725	38.0	33.0	40.0	23.0	41.0
37	36.02475	38.0	35.0	40.0	30.0	41.0
38	37.32325	39.0	37.0	40.0	32.0	41.0
39	37.2525	39.0	37.0	40.0	32.0	41.0
40	37.3575	39.0	37.0	41.0	32.0	41.0
41	37.31975	40.0	37.0	41.0	32.0	41.0
42	37.451	40.0	37.0	41.0	33.0	41.0
43	37.23975	40.0	37.0	41.0	32.0	41.0
44	37.199	40.0	37.0	41.0	32.0	41.0
45	36.88025	39.0	37.0	41.0	31.0	41.0
46	35.188	38.0	34.0	40.0	26.0	41.0
47	35.94925	39.0	35.0	40.0	29.0	41.0
48	36.276	39.0	36.0	41.0	30.0	41.0
49	35.36075	39.0	35.0	41.0	28.0	41.0
50	30.715	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	2.0
17	1.0
18	2.0
19	4.0
20	4.0
21	4.0
22	8.0
23	6.0
24	8.0
25	12.0
26	25.0
27	14.0
28	32.0
29	38.0
30	51.0
31	70.0
32	73.0
33	96.0
34	142.0
35	183.0
36	283.0
37	477.0
38	1030.0
39	1434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.942885771543088	23.597194388777556	52.980961923847694	14.478957915831664
2	21.2	26.1	19.2	33.5
3	25.8	20.1	20.825	33.275
4	30.375000000000004	27.875	13.725000000000001	28.025
5	18.4	43.9	24.675	13.025
6	35.5	17.349999999999998	33.800000000000004	13.350000000000001
7	15.65	18.8	26.6	38.95
8	25.15	29.975	14.774999999999999	30.099999999999998
9	23.724999999999998	36.4	26.650000000000002	13.225000000000001
10	22.125	21.15	31.15	25.575
11	25.575	12.2	17.075000000000003	45.15
12	13.675	18.099999999999998	33.300000000000004	34.925
13	33.775	19.45	28.15	18.625
14	30.45	34.25	19.8	15.5
15	35.075	25.474999999999998	25.0	14.45
16	16.150000000000002	23.674999999999997	19.75	40.425
17	20.525	21.15	42.975	15.35
18	23.849999999999998	19.525000000000002	31.775	24.85
19	38.775	12.75	29.975	18.5
20	42.85	14.75	28.299999999999997	14.099999999999998
21	22.475	18.975	29.099999999999998	29.45
22	28.65	12.3	50.075	8.975
23	28.475	12.075	45.1	14.35
24	53.2	15.325	23.9	7.575
25	49.425000000000004	26.650000000000002	13.625000000000002	10.299999999999999
26	24.9	48.9	15.25	10.95
27	14.149999999999999	48.1	26.75	11.0
28	11.425	23.0	50.475	15.1
29	9.375	12.950000000000001	58.8	18.875
30	9.025	7.35	35.975	47.65
31	15.2	5.625	47.075	32.1
32	30.175	6.075	19.625	44.125
33	57.599999999999994	8.625	12.75	21.025
34	62.1	7.425	16.2	14.274999999999999
35	62.975	8.1	19.975	8.95
36	42.575	7.9	35.0	14.524999999999999
37	45.425	9.225	18.55	26.8
38	21.224999999999998	16.225	11.825	50.724999999999994
39	15.25	27.224999999999998	7.6	49.925000000000004
40	15.25	50.724999999999994	10.475	23.549999999999997
41	27.500000000000004	49.575	7.75	15.174999999999999
42	51.349999999999994	30.45	9.775	8.425
43	49.975	33.675	7.7	8.649999999999999
44	27.025	52.349999999999994	6.15	14.475
45	16.125	51.349999999999994	12.925	19.6
46	10.05	26.75	19.625	43.575
47	7.124999999999999	16.55	39.675	36.65
48	9.575	14.799999999999999	18.35	57.275
49	14.549999999999999	21.625	11.75	52.075
50	20.005001250312578	38.20955238809702	13.42835708927232	28.35708927231808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.0
28	1.0
29	1.5
30	2.0
31	11.5
32	21.0
33	18.0
34	15.0
35	27.0
36	39.0
37	46.0
38	53.0
39	49.0
40	45.0
41	48.0
42	51.0
43	85.5
44	120.0
45	163.5
46	207.0
47	324.0
48	441.0
49	570.0
50	699.0
51	769.5
52	840.0
53	642.5
54	445.0
55	467.0
56	489.0
57	369.0
58	249.0
59	156.0
60	63.0
61	52.5
62	42.0
63	58.5
64	75.0
65	62.0
66	49.0
67	29.5
68	10.0
69	9.5
70	9.0
71	16.5
72	24.0
73	12.5
74	1.0
75	1.0
76	1.0
77	2.0
78	3.0
79	2.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79045776918117	28.999999999999996
2	10.251450676982591	7.95
3	3.9329464861379755	4.575
4	2.4500322372662797	3.8
5	1.5473887814313347	3.0
6	0.9671179883945842	2.25
7	0.9671179883945842	2.625
8	0.6447453255963894	2.0
9	0.4513217279174726	1.575
>10	3.481624758220503	24.275
>50	0.2578981302385558	7.124999999999999
>100	0.2578981302385558	11.825
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	132	3.3000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	124	3.1	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	116	2.9000000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	83	2.075	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	79	1.975	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	65	1.625	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	45	1.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	40	1.0	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	39	0.975	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	39	0.975	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	25	0.625	RNA PCR Primer, Index 7 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	24	0.6	RNA PCR Primer, Index 1 (100% over 33bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	18	0.44999999999999996	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTC	16	0.4	RNA PCR Primer, Index 28 (100% over 44bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	12	0.3	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	10	0.25	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	10	0.25	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	9	0.22499999999999998	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	9	0.22499999999999998	No Hit
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	8	0.2	No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	8	0.2	RNA PCR Primer, Index 7 (100% over 35bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGC	8	0.2	RNA PCR Primer, Index 28 (100% over 50bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	6	0.15	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAG	5	0.125	RNA PCR Primer, Index 28 (100% over 39bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGGGCTAGGGCTAACAGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	5	0.125	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATGGAAT	5	0.125	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	5	0.125	No Hit
AGATCAGAAGGTGACTGTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTAT	5	0.125	RNA PCR Primer, Index 28 (100% over 48bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.325	0.0	0.0	0.0
2	0.0	0.35	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.575	0.0	0.0	0.0
5	0.0	0.65	0.0	0.0	0.0
6	0.0	0.75	0.0	0.0	0.0
7	0.0	1.375	0.0	0.0	0.0
8	0.0	1.6	0.0	0.0	0.0
9	0.0	1.675	0.0	0.0	0.0
10	0.0	1.8	0.0	0.0	0.0
11	0.0	2.1	0.0	0.0	0.0
12	0.0	2.575	0.0	0.0	0.0
13	0.0	2.85	0.0	0.0	0.0
14	0.0	3.15	0.0	0.0	0.0
15	0.0	3.525	0.0	0.0	0.0
16	0.0	4.65	0.0	0.0	0.0
17	0.0	5.1	0.0	0.0	0.0
18	0.0	6.35	0.0	0.0	0.0
19	0.0	6.825	0.0	0.0	0.0
20	0.0	7.725	0.0	0.0	0.0
21	0.0	14.4	0.0	0.0	0.0
22	0.0	27.3	0.0	0.0	0.0
23	0.0	58.175	0.0	0.0	0.0
24	0.0	69.575	0.0	0.0	0.0
25	0.0	75.3	0.0	0.0	0.0
26	0.0	76.575	0.0	0.0	0.0
27	0.0	77.375	0.0	0.0	0.0
28	0.0	77.65	0.0	0.0	0.0
29	0.0	78.325	0.0	0.0	0.0
30	0.0	78.825	0.0	0.0	0.0
31	0.0	79.375	0.0	0.0	0.0
32	0.0	80.025	0.0	0.0	0.0
33	0.0	80.725	0.0	0.0	0.0
34	0.0	82.625	0.0	0.0	0.0
35	0.0	83.325	0.0	0.0	0.0
36	0.0	83.6	0.0	0.0	0.0
37	0.0	84.425	0.0	0.0	0.0
38	0.0	84.825	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AATCCAG	20	6.952051E-4	44.000004	8
ATCCAGG	20	6.952051E-4	44.000004	9
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
ATAGGCT	20	6.952051E-4	44.000004	16
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
TCGGATC	45	2.728484E-10	44.000004	1
CGGATCC	45	2.728484E-10	44.000004	2
TCCAGGA	20	6.952051E-4	44.000004	10
GTGAGTT	20	6.952051E-4	44.000004	18
GAGTTGG	20	6.952051E-4	44.000004	20
GGATAGG	20	6.952051E-4	44.000004	14
AGTAATC	20	6.952051E-4	44.000004	5
GATCCGT	45	2.728484E-10	44.000004	4
TAATCCA	20	6.952051E-4	44.000004	7
TTGGCTT	20	6.952051E-4	44.000004	17
>>END_MODULE
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216344 spots for SRR5030839.sra
Written 216344 spots for SRR5030839.sra
Read 216346 spots for SRR5030839.sra
Written 216346 spots for SRR5030839.sra
SRR ids: ['SRR5030839.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gedyx01o
SRR5030839.sra spots: 2596130
blocks: [[1, 216344], [216345, 432688], [432689, 649032], [649033, 865376], [865377, 1081720], [1081721, 1298064], [1298065, 1514408], [1514409, 1730752], [1730753, 1947096], [1947097, 2163440], [2163441, 2379784], [2379785, 2596130]]
SRR5030839 file size 448889
SRR5030839 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030839 SRR5030839_1.fastq
Input file:	SRR5030839_1.fastq
trimmed:	SRR5030839-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:24:28 2020 >> started

Wed Mar  4 06:24:29 2020 >> done (1.471s)
2596130 reads processed; of these:
    108 ( 0.00%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
2596017 (100.00%) reads available; of these:
 137193 ( 5.28%) trimmed reads available after processing
2458824 (94.72%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     43	  0.00%
 19	     54	  0.00%
 20	    150	  0.01%
 21	    156	  0.01%
 22	    281	  0.01%
 23	    273	  0.01%
 24	    309	  0.01%
 25	    398	  0.02%
 26	    300	  0.01%
 27	    461	  0.02%
 28	    551	  0.02%
 29	    862	  0.03%
 30	    600	  0.02%
 31	    799	  0.03%
 32	    706	  0.03%
 33	   1071	  0.04%
 34	   2176	  0.08%
 35	   2135	  0.08%
 36	   1604	  0.06%
 37	   1844	  0.07%
 38	   1912	  0.07%
 39	   2142	  0.08%
 40	   2212	  0.09%
 41	   2652	  0.10%
 42	   3451	  0.13%
 43	   4520	  0.17%
 44	   8342	  0.32%
 45	   9688	  0.37%
 46	   9077	  0.35%
 47	  12815	  0.49%
 48	  26735	  1.03%
 49	  38874	  1.50%
 50	2458824	 94.72%
2596017 reads passed initial QC


criterion=sequence-density
sequence-density=84.42
sequence-density-rank=1
fanout-score=18.48
fanout-score-rank=1
prefix-density=85.86
prefix-fanout=18.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGCC


criterion=fanout-score
sequence-density=84.42
sequence-density-rank=1
fanout-score=18.48
fanout-score-rank=1
prefix-density=85.86
prefix-fanout=18.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGCC -o SRR5030839 -
Input file:	STDIN
trimmed:	SRR5030839-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:24:43 2020 >> started

Wed Mar  4 06:24:46 2020 >> done (2.691s)
2534934 reads processed; of these:
 138505 ( 5.46%) short reads filtered out after trimming by size control
  11137 ( 0.44%) empty reads filtered out after trimming by size control
2385292 (94.10%) reads available; of these:
2174086 (91.15%) trimmed reads available after processing
 211206 ( 8.85%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13693	  0.57%
 19	  24809	  1.04%
 20	 158445	  6.64%
 21	 322959	 13.54%
 22	 790207	 33.13%
 23	 312286	 13.09%
 24	 155012	  6.50%
 25	  38075	  1.60%
 26	  18249	  0.77%
 27	  13618	  0.57%
 28	  13824	  0.58%
 29	   9881	  0.41%
 30	  15640	  0.66%
 31	  21326	  0.89%
 32	  18511	  0.78%
 33	  49814	  2.09%
 34	  17484	  0.73%
 35	  13958	  0.59%
 36	  15367	  0.64%
 37	   9319	  0.39%
 38	   9838	  0.41%
 39	  10339	  0.43%
 40	  15053	  0.63%
 41	  21599	  0.91%
 42	  26296	  1.10%
 43	  17606	  0.74%
 44	  14654	  0.61%
 45	  17964	  0.75%
 46	  12527	  0.53%
 47	   9838	  0.41%
 48	   2309	  0.10%
 49	   7903	  0.33%
 50	 186889	  7.84%


criterion=sequence-density
sequence-density=4.70
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=18
prefix-density=0.00
prefix-fanout=1.0
sequence=AAGCTGCCAGTTGAAGAACTGT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=38
fanout-score=47.32
fanout-score-rank=1
prefix-density=1.12
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:25:05
                             Started mapping on |	Mar 04 06:25:06
                                    Finished on |	Mar 04 06:25:29
       Mapping speed, Million of reads per hour |	382.91

                          Number of input reads |	2446375
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1461767
                        Uniquely mapped reads % |	59.75%
                          Average mapped length |	26.02
                       Number of splices: Total |	867
            Number of splices: Annotated (sjdb) |	128
                       Number of splices: GT/AG |	782
                       Number of splices: GC/AG |	9
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	74
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.61
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	751844
             % of reads mapped to multiple loci |	30.73%
        Number of reads mapped to too many loci |	38272
             % of reads mapped to too many loci |	1.56%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.48%
                     % of reads unmapped: other |	0.47%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	232764	232764	232764
N_multimapping	751844	751844	751844
N_noFeature	54922	67690	1001375
N_ambiguous	841927	442352	12894
UnstrandedReadsAssigned:564918 PositiveStrandReadsAssigned:951725 NegativeStrandReadsAssigned:447498
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030839 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030839-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,446,375 reads, 697,837 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 633 rounds

 109283 SRR5030839.ke.tsv
  52636 SRR5030839.se.tsv
 161919 total
==> SRR5030839.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030839.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030839 completed mapping pipeline successfully
