Starting /dee2/code/volunteer_pipeline.sh SRR5030845
    current disk space = 160681263104
    free memory = 73906132 
SRR5030845 SRAfilesize
09eb15a0b05737e9d43666e1a4c4656f  SRR5030845.sra
SRR5030845.sra file validated
SRR5030845 is single end
SRR5030845 is conventional basespace
SRR5030845 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.521	34.0	31.0	34.0	31.0	34.0
2	32.7705	34.0	31.0	34.0	31.0	34.0
3	32.8565	34.0	31.0	34.0	31.0	34.0
4	36.2875	37.0	37.0	37.0	35.0	37.0
5	36.3705	37.0	37.0	37.0	35.0	37.0
6	36.17225	37.0	37.0	37.0	35.0	37.0
7	36.1435	37.0	35.0	37.0	35.0	37.0
8	36.2015	37.0	37.0	37.0	35.0	37.0
9	38.04375	39.0	38.0	39.0	35.0	39.0
10	38.05025	39.0	39.0	39.0	35.0	39.0
11	37.981	39.0	38.0	39.0	35.0	39.0
12	37.96025	39.0	38.0	39.0	35.0	39.0
13	37.9165	39.0	38.0	39.0	35.0	39.0
14	39.3235	41.0	39.0	41.0	36.0	41.0
15	39.226	40.0	39.0	41.0	36.0	41.0
16	39.235	40.0	39.0	41.0	36.0	41.0
17	39.37375	41.0	39.0	41.0	36.0	41.0
18	39.3645	41.0	39.0	41.0	36.0	41.0
19	39.20375	41.0	39.0	41.0	36.0	41.0
20	39.08575	40.0	39.0	41.0	36.0	41.0
21	39.02125	40.0	39.0	41.0	36.0	41.0
22	38.70925	40.0	38.0	41.0	34.0	41.0
23	38.57275	40.0	38.0	41.0	34.0	41.0
24	38.47225	40.0	38.0	41.0	34.0	41.0
25	38.8865	40.0	39.0	41.0	35.0	41.0
26	39.2045	40.0	39.0	41.0	36.0	41.0
27	39.246	41.0	39.0	41.0	36.0	41.0
28	39.01775	40.0	39.0	41.0	35.0	41.0
29	38.4145	40.0	38.0	41.0	34.0	41.0
30	38.58225	40.0	38.0	41.0	34.0	41.0
31	38.84175	40.0	38.0	41.0	35.0	41.0
32	39.00975	40.0	39.0	41.0	35.0	41.0
33	38.242	40.0	38.0	41.0	33.0	41.0
34	38.21225	40.0	38.0	41.0	33.0	41.0
35	38.0695	40.0	38.0	41.0	34.0	41.0
36	34.80125	38.0	33.0	40.0	23.0	41.0
37	36.12525	38.0	35.0	40.0	30.0	41.0
38	37.37175	39.0	37.0	40.0	33.0	41.0
39	37.402	39.0	37.0	40.0	33.0	41.0
40	37.3635	40.0	37.0	41.0	33.0	41.0
41	37.36675	40.0	37.0	41.0	32.0	41.0
42	37.55875	40.0	37.0	41.0	33.0	41.0
43	37.289	40.0	37.0	41.0	32.0	41.0
44	37.30675	40.0	37.0	41.0	33.0	41.0
45	37.043	39.0	37.0	41.0	32.0	41.0
46	35.38825	38.0	34.0	40.0	27.0	41.0
47	35.88225	39.0	35.0	40.0	29.0	41.0
48	36.1825	39.0	36.0	40.0	30.0	41.0
49	35.1625	39.0	35.0	40.0	28.0	41.0
50	30.67575	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	4.0
20	5.0
21	3.0
22	4.0
23	8.0
24	12.0
25	13.0
26	12.0
27	18.0
28	30.0
29	30.0
30	58.0
31	64.0
32	74.0
33	111.0
34	148.0
35	190.0
36	312.0
37	540.0
38	1022.0
39	1339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.16145181476846	23.379224030037545	52.56570713391739	14.893617021276595
2	22.05	26.450000000000003	18.75	32.75
3	26.3	20.575	22.025	31.1
4	30.175	28.875	14.35	26.6
5	19.8	44.525	24.3	11.375
6	34.475	17.175	34.475	13.875000000000002
7	15.45	20.325	25.6	38.625
8	25.3	31.1	14.05	29.549999999999997
9	22.5	34.025	29.675	13.8
10	20.974999999999998	21.675	30.975	26.375
11	26.950000000000003	12.375	17.75	42.925000000000004
12	14.45	17.45	32.225	35.875
13	31.4	21.325	29.125	18.15
14	31.275	33.2	20.4	15.125
15	34.875	25.7	25.974999999999998	13.450000000000001
16	17.0	22.45	20.95	39.6
17	21.475	21.8	42.1	14.625
18	23.95	19.875	31.05	25.124999999999996
19	39.025	13.725000000000001	30.049999999999997	17.2
20	42.85	16.75	27.224999999999998	13.175
21	22.375	19.75	28.375	29.5
22	28.000000000000004	12.6	48.825	10.575
23	26.924999999999997	13.525	44.5	15.049999999999999
24	50.64999999999999	15.950000000000001	24.85	8.55
25	49.0	25.650000000000002	15.325	10.025
26	27.900000000000002	46.45	14.499999999999998	11.15
27	15.725	47.05	25.525	11.700000000000001
28	11.175	25.2	48.85	14.774999999999999
29	9.975000000000001	13.900000000000002	57.125	19.0
30	9.725	6.825	37.025000000000006	46.425
31	14.899999999999999	6.075	46.35	32.675
32	28.249999999999996	6.4750000000000005	20.599999999999998	44.675
33	54.825	7.6499999999999995	14.124999999999998	23.400000000000002
34	60.824999999999996	7.95	15.675	15.55
35	63.975	7.324999999999999	18.325	10.375
36	43.6	7.95	34.599999999999994	13.850000000000001
37	45.25	10.424999999999999	18.775	25.55
38	22.125	16.275000000000002	13.025	48.575
39	16.650000000000002	25.15	9.1	49.1
40	15.35	48.575	10.575	25.5
41	26.650000000000002	48.725	8.450000000000001	16.175
42	50.449999999999996	30.9	9.6	9.049999999999999
43	49.125	32.800000000000004	9.075	9.0
44	28.825	50.324999999999996	7.199999999999999	13.65
45	17.974999999999998	50.075	13.5	18.45
46	11.075	27.55	18.95	42.425000000000004
47	8.924999999999999	16.1	38.625	36.35
48	9.775	13.850000000000001	19.950000000000003	56.425000000000004
49	14.249999999999998	19.8	14.325	51.625
50	20.349999999999998	35.85	13.100000000000001	30.7
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	0.5
18	0.0
19	1.0
20	2.0
21	1.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	1.0
30	1.0
31	7.5
32	14.0
33	18.5
34	23.0
35	30.5
36	38.0
37	51.0
38	64.0
39	57.0
40	50.0
41	50.0
42	50.0
43	94.5
44	139.0
45	183.0
46	227.0
47	351.0
48	475.0
49	573.5
50	672.0
51	733.0
52	794.0
53	594.0
54	394.0
55	438.0
56	482.0
57	366.0
58	250.0
59	166.0
60	82.0
61	64.0
62	46.0
63	66.5
64	87.0
65	60.0
66	33.0
67	24.5
68	16.0
69	14.5
70	13.0
71	21.5
72	30.0
73	18.0
74	6.0
75	3.0
76	0.0
77	1.0
78	2.0
79	3.0
80	4.0
81	3.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.98427260812582	28.225
2	10.747051114023591	8.200000000000001
3	3.473132372214941	3.975
4	2.686762778505898	4.1000000000000005
5	1.6382699868938402	3.125
6	1.507208387942333	3.45
7	1.1140235910878113	2.9749999999999996
8	0.5897771952817824	1.7999999999999998
9	0.8519003931847969	2.9250000000000003
>10	2.8833551769331587	21.95
>50	0.2621231979030144	7.124999999999999
>100	0.2621231979030144	12.15
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	147	3.675	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	127	3.175	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	110	2.75	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	102	2.55	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	84	2.1	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	80	2.0	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	65	1.625	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	56	1.4000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	47	1.175	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	45	1.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	40	1.0	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC	25	0.625	RNA PCR Primer, Index 37 (100% over 44bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	22	0.5499999999999999	RNA PCR Primer, Index 2 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21	0.525	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	15	0.375	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 37 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	10	0.25	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	10	0.25	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	9	0.22499999999999998	No Hit
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	9	0.22499999999999998	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	9	0.22499999999999998	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	9	0.22499999999999998	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	8	0.2	RNA PCR Primer, Index 1 (100% over 32bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	7	0.17500000000000002	No Hit
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	6	0.15	No Hit
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGAAATGCGGATATGGGACTGAGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TACAGTACTGTGATAACTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGA	5	0.125	RNA PCR Primer, Index 7 (97% over 37bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ACCTTGGCTCTAGACTGCTTACTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	5	0.125	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.35	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.625	0.0	0.0	0.0
5	0.0	0.7	0.0	0.0	0.0
6	0.0	0.825	0.0	0.0	0.0
7	0.0	1.625	0.0	0.0	0.0
8	0.0	1.725	0.0	0.0	0.0
9	0.0	1.85	0.0	0.0	0.0
10	0.0	1.95	0.0	0.0	0.0
11	0.0	2.075	0.0	0.0	0.0
12	0.0	2.25	0.0	0.0	0.0
13	0.0	2.35	0.0	0.0	0.0
14	0.0	2.65	0.0	0.0	0.0
15	0.0	3.075	0.0	0.0	0.0
16	0.0	4.15	0.0	0.0	0.0
17	0.0	4.85	0.0	0.0	0.0
18	0.0	5.85	0.0	0.0	0.0
19	0.0	6.45	0.0	0.0	0.0
20	0.0	7.5	0.0	0.0	0.0
21	0.0	13.675	0.0	0.0	0.0
22	0.0	25.475	0.0	0.0	0.0
23	0.0	55.0	0.0	0.0	0.0
24	0.0	67.175	0.0	0.0	0.0
25	0.0	73.6	0.0	0.0	0.0
26	0.0	74.9	0.0	0.0	0.0
27	0.0	75.675	0.0	0.0	0.0
28	0.0	76.125	0.0	0.0	0.0
29	0.0	76.55	0.0	0.0	0.0
30	0.0	77.025	0.0	0.0	0.0
31	0.0	77.8	0.0	0.0	0.0
32	0.0	78.425	0.0	0.0	0.0
33	0.0	79.05	0.0	0.0	0.0
34	0.0	81.175	0.0	0.0	0.0
35	0.0	82.025	0.0	0.0	0.0
36	0.0	82.45	0.0	0.0	0.0
37	0.0	83.2	0.0	0.0	0.0
38	0.0	83.6	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
GAGGTAG	20	6.952051E-4	44.000004	2
GTTGAAG	20	6.952051E-4	44.000004	10
CTGAGAC	20	6.952051E-4	44.000004	4
TAAACAT	20	6.952051E-4	44.000004	3
CTTTAAC	20	6.952051E-4	44.000004	12
CCAGTTG	20	6.952051E-4	44.000004	7
AAACATC	20	6.952051E-4	44.000004	4
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
TCGGATC	45	2.728484E-10	44.000004	1
AACCTGT	20	6.952051E-4	44.000004	16
CGGATCC	45	2.728484E-10	44.000004	2
GAAGAAC	20	6.952051E-4	44.000004	13
GCCAGTT	20	6.952051E-4	44.000004	6
ACATCCT	20	6.952051E-4	44.000004	6
TAACCTG	20	6.952051E-4	44.000004	15
GCTGCCA	20	6.952051E-4	44.000004	3
GATCCGT	45	2.728484E-10	44.000004	4
>>END_MODULE
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249178 spots for SRR5030845.sra
Written 249178 spots for SRR5030845.sra
Read 249183 spots for SRR5030845.sra
Written 249183 spots for SRR5030845.sra
SRR ids: ['SRR5030845.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7arhv1yq
SRR5030845.sra spots: 2990141
blocks: [[1, 249178], [249179, 498356], [498357, 747534], [747535, 996712], [996713, 1245890], [1245891, 1495068], [1495069, 1744246], [1744247, 1993424], [1993425, 2242602], [2242603, 2491780], [2491781, 2740958], [2740959, 2990141]]
SRR5030845 file size 517182
SRR5030845 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030845 SRR5030845_1.fastq
Input file:	SRR5030845_1.fastq
trimmed:	SRR5030845-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:36 2020 >> started

Wed Mar  4 06:26:38 2020 >> done (2.194s)
2990141 reads processed; of these:
    133 ( 0.00%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2990002 (100.00%) reads available; of these:
 175113 ( 5.86%) trimmed reads available after processing
2814889 (94.14%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     53	  0.00%
 19	     56	  0.00%
 20	    148	  0.00%
 21	    137	  0.00%
 22	    265	  0.01%
 23	    307	  0.01%
 24	    345	  0.01%
 25	    430	  0.01%
 26	    370	  0.01%
 27	    534	  0.02%
 28	    676	  0.02%
 29	    906	  0.03%
 30	    729	  0.02%
 31	   1023	  0.03%
 32	   1002	  0.03%
 33	   1219	  0.04%
 34	   2477	  0.08%
 35	   2189	  0.07%
 36	   1775	  0.06%
 37	   1897	  0.06%
 38	   2224	  0.07%
 39	   2715	  0.09%
 40	   2963	  0.10%
 41	   3135	  0.10%
 42	   4440	  0.15%
 43	   5262	  0.18%
 44	   9549	  0.32%
 45	  12937	  0.43%
 46	  11925	  0.40%
 47	  17246	  0.58%
 48	  36342	  1.22%
 49	  49837	  1.67%
 50	2814889	 94.14%
2990002 reads passed initial QC


criterion=sequence-density
sequence-density=82.41
sequence-density-rank=1
fanout-score=19.20
fanout-score-rank=1
prefix-density=83.89
prefix-fanout=18.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC


criterion=fanout-score
sequence-density=82.41
sequence-density-rank=1
fanout-score=19.20
fanout-score-rank=1
prefix-density=83.89
prefix-fanout=18.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC -o SRR5030845 -
Input file:	STDIN
trimmed:	SRR5030845-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:57 2020 >> started

Wed Mar  4 06:27:03 2020 >> done (5.687s)
2917954 reads processed; of these:
 173056 ( 5.93%) short reads filtered out after trimming by size control
  14480 ( 0.50%) empty reads filtered out after trimming by size control
2730418 (93.57%) reads available; of these:
2452886 (89.84%) trimmed reads available after processing
 277532 (10.16%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  17478	  0.64%
 19	  29644	  1.09%
 20	 168723	  6.18%
 21	 361079	 13.22%
 22	 852261	 31.21%
 23	 355879	 13.03%
 24	 180012	  6.59%
 25	  45118	  1.65%
 26	  22727	  0.83%
 27	  16984	  0.62%
 28	  17836	  0.65%
 29	  12933	  0.47%
 30	  18800	  0.69%
 31	  22199	  0.81%
 32	  22854	  0.84%
 33	  59450	  2.18%
 34	  19726	  0.72%
 35	  15798	  0.58%
 36	  17720	  0.65%
 37	  12376	  0.45%
 38	  12520	  0.46%
 39	  13608	  0.50%
 40	  21311	  0.78%
 41	  28577	  1.05%
 42	  32858	  1.20%
 43	  23078	  0.85%
 44	  18987	  0.70%
 45	  23691	  0.87%
 46	  17457	  0.64%
 47	  13178	  0.48%
 48	   3290	  0.12%
 49	   9646	  0.35%
 50	 242620	  8.89%


criterion=sequence-density
sequence-density=4.93
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=37
fanout-score=77.94
fanout-score-rank=1
prefix-density=1.27
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:27:34
                             Started mapping on |	Mar 04 06:27:35
                                    Finished on |	Mar 04 06:27:59
       Mapping speed, Million of reads per hour |	420.37

                          Number of input reads |	2802466
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1647308
                        Uniquely mapped reads % |	58.78%
                          Average mapped length |	26.50
                       Number of splices: Total |	1128
            Number of splices: Annotated (sjdb) |	162
                       Number of splices: GT/AG |	1034
                       Number of splices: GC/AG |	12
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	81
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.61
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	857959
             % of reads mapped to multiple loci |	30.61%
        Number of reads mapped to too many loci |	45338
             % of reads mapped to too many loci |	1.62%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.49%
                     % of reads unmapped: other |	0.49%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	297199	297199	297199
N_multimapping	857959	857959	857959
N_noFeature	62098	77966	1159055
N_ambiguous	920397	496413	17318
UnstrandedReadsAssigned:664813 PositiveStrandReadsAssigned:1072929 NegativeStrandReadsAssigned:470935
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030845 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030845-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,802,466 reads, 760,813 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,095 rounds

 109283 SRR5030845.ke.tsv
  52636 SRR5030845.se.tsv
 161919 total
==> SRR5030845.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030845.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030845 completed mapping pipeline successfully
