Starting /dee2/code/volunteer_pipeline.sh SRR5031046
    current disk space = 160488046592
    free memory = 73930444 
SRR5031046 SRAfilesize
d15c4a5e3b2797609be5b42ad32fd105  SRR5031046.sra
SRR5031046.sra file validated
SRR5031046 is single end
SRR5031046 is conventional basespace
SRR5031046 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2315	33.0	31.0	34.0	30.0	34.0
2	32.48075	34.0	31.0	34.0	31.0	34.0
3	32.53275	34.0	31.0	34.0	31.0	34.0
4	36.13275	37.0	35.0	37.0	35.0	37.0
5	35.86475	37.0	35.0	37.0	35.0	37.0
6	35.7775	37.0	35.0	37.0	33.0	37.0
7	35.8815	37.0	35.0	37.0	35.0	37.0
8	35.7265	37.0	35.0	37.0	33.0	37.0
9	37.43825	39.0	37.0	39.0	34.0	39.0
10	37.65025	39.0	37.0	39.0	35.0	39.0
11	37.58025	39.0	37.0	39.0	35.0	39.0
12	37.48225	39.0	37.0	39.0	34.0	39.0
13	37.51375	39.0	37.0	39.0	35.0	39.0
14	38.85925	40.0	38.0	41.0	35.0	41.0
15	38.74825	40.0	38.0	41.0	34.0	41.0
16	38.80475	40.0	38.0	41.0	35.0	41.0
17	38.80575	40.0	38.0	41.0	34.0	41.0
18	38.9535	40.0	38.0	41.0	35.0	41.0
19	38.37425	40.0	38.0	41.0	34.0	41.0
20	38.65025	40.0	38.0	41.0	34.0	41.0
21	38.568	40.0	38.0	41.0	34.0	41.0
22	38.8555	40.0	38.0	41.0	35.0	41.0
23	38.58725	40.0	38.0	41.0	34.0	41.0
24	38.49425	40.0	38.0	41.0	34.0	41.0
25	38.8075	40.0	38.0	41.0	35.0	41.0
26	38.466	40.0	38.0	41.0	34.0	41.0
27	38.4115	40.0	38.0	41.0	34.0	41.0
28	38.52125	40.0	38.0	41.0	34.0	41.0
29	38.062	40.0	38.0	41.0	33.0	41.0
30	37.87275	40.0	37.0	41.0	32.0	41.0
31	38.1165	40.0	38.0	41.0	33.0	41.0
32	37.88825	40.0	37.0	41.0	33.0	41.0
33	37.523	40.0	37.0	41.0	32.0	41.0
34	37.51325	40.0	37.0	41.0	32.0	41.0
35	37.577	39.0	37.0	40.0	33.0	41.0
36	37.26425	39.0	37.0	40.0	31.0	41.0
37	37.1545	39.0	36.0	40.0	31.0	41.0
38	36.30575	39.0	35.0	40.0	30.0	41.0
39	36.8485	39.0	36.0	40.0	31.0	41.0
40	37.05925	39.0	37.0	40.0	31.0	41.0
41	37.23425	40.0	36.0	41.0	31.0	41.0
42	37.48375	40.0	37.0	41.0	32.0	41.0
43	37.79375	40.0	37.0	41.0	33.0	41.0
44	37.5885	40.0	37.0	41.0	33.0	41.0
45	37.4725	40.0	37.0	41.0	32.0	41.0
46	37.2265	40.0	36.0	41.0	32.0	41.0
47	36.579	40.0	35.0	41.0	30.0	41.0
48	36.1015	39.0	35.0	41.0	29.0	41.0
49	36.379	39.0	35.0	41.0	30.0	41.0
50	35.96025	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	4.0
16	0.0
17	0.0
18	1.0
19	1.0
20	4.0
21	8.0
22	3.0
23	10.0
24	10.0
25	12.0
26	15.0
27	20.0
28	40.0
29	41.0
30	54.0
31	76.0
32	86.0
33	129.0
34	163.0
35	229.0
36	312.0
37	444.0
38	743.0
39	1593.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.8	24.8	46.375	19.025
2	23.916854495366895	26.69671925870273	18.682694715752568	30.703731530177812
3	24.075	19.7	21.4	34.825
4	32.5	27.450000000000003	15.6	24.45
5	24.2	35.925000000000004	26.775	13.100000000000001
6	34.699999999999996	13.5	34.675	17.125
7	17.575	22.95	21.6	37.875
8	24.075	27.525	17.375	31.025000000000002
9	23.549999999999997	31.4	27.6	17.45
10	22.55	22.775000000000002	26.0	28.675
11	33.775	11.200000000000001	16.475	38.550000000000004
12	16.2	18.425	30.25	35.125
13	29.275000000000002	17.150000000000002	32.85	20.724999999999998
14	29.975	29.9	20.474999999999998	19.650000000000002
15	35.4	23.625	25.95	15.024999999999999
16	19.55	21.175	23.400000000000002	35.875
17	27.1	22.85	34.25	15.8
18	23.75	18.025	29.875	28.349999999999998
19	35.875	14.45	29.675	20.0
20	43.05	13.950000000000001	28.849999999999998	14.149999999999999
21	24.925	20.325	26.025	28.725
22	29.25	14.000000000000002	45.2	11.55
23	27.450000000000003	13.100000000000001	42.325	17.125
24	49.925000000000004	15.35	25.45	9.275
25	48.125	23.974999999999998	16.35	11.55
26	27.85	43.55	14.524999999999999	14.075
27	16.8	44.7	23.474999999999998	15.024999999999999
28	12.1	23.875	46.225	17.8
29	14.000000000000002	13.925	53.974999999999994	18.099999999999998
30	10.525	7.675	34.825	46.975
31	16.575	6.175	45.800000000000004	31.45
32	29.075	6.7250000000000005	20.474999999999998	43.725
33	51.025	11.4	14.524999999999999	23.05
34	57.3	9.0	18.0	15.7
35	62.45	7.5	20.025000000000002	10.025
36	41.05	7.9750000000000005	35.6	15.375
37	43.6	12.2	20.45	23.75
38	23.1	18.125	13.275	45.5
39	16.875	23.474999999999998	12.275	47.375
40	17.925	44.65	13.15	24.275
41	25.05	45.75	11.15	18.05
42	46.35	28.325	13.05	12.275
43	49.975	27.55	9.950000000000001	12.525
44	31.65	44.65	8.85	14.85
45	21.325	46.175	13.375	19.125
46	15.7	25.25	18.125	40.925
47	10.8	15.525	38.574999999999996	35.099999999999994
48	13.125	12.55	21.5	52.825
49	16.2	17.275	14.774999999999999	51.74999999999999
50	19.5	35.225	13.700000000000001	31.574999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	1.0
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	1.5
30	3.0
31	11.5
32	20.0
33	15.5
34	11.0
35	21.0
36	31.0
37	37.0
38	43.0
39	47.0
40	51.0
41	42.5
42	34.0
43	64.0
44	94.0
45	133.5
46	173.0
47	276.0
48	379.0
49	458.0
50	537.0
51	689.0
52	841.0
53	635.5
54	430.0
55	481.0
56	532.0
57	402.5
58	273.0
59	198.0
60	123.0
61	95.0
62	67.0
63	113.5
64	160.0
65	99.5
66	39.0
67	29.5
68	20.0
69	28.0
70	36.0
71	61.5
72	87.0
73	46.0
74	5.0
75	4.5
76	4.0
77	2.0
78	0.0
79	1.0
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71116816431322	29.099999999999998
2	10.526315789473683	8.200000000000001
3	4.10783055198973	4.8
4	2.1181001283697047	3.3000000000000003
5	1.9255455712451863	3.75
6	1.1553273427471118	2.7
7	1.0911424903722722	2.9749999999999996
8	0.25673940949935814	0.8
9	0.7060333761232349	2.475
>10	2.8883183568677793	22.25
>50	0.3209242618741977	11.05
>100	0.19255455712451863	8.6
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	120	3.0	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	113	2.825	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	111	2.775	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	100	2.5	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	97	2.4250000000000003	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	95	2.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	88	2.1999999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	47	1.175	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	45	1.125	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	30	0.75	RNA PCR Primer, Index 1 (97% over 35bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	25	0.625	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC	23	0.575	RNA PCR Primer, Index 18 (100% over 50bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	16	0.4	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	14	0.35000000000000003	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 31bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC	12	0.3	RNA PCR Primer, Index 18 (100% over 44bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	11	0.27499999999999997	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	10	0.25	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGAATTGTGGCTGGACATCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	6	0.15	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	6	0.15	No Hit
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	6	0.15	No Hit
AGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGAGCGGGCACCACTGCACGAGAGTAGGCCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC	5	0.125	RNA PCR Primer, Index 18 (100% over 39bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.65	0.0	0.0	0.0
2	0.0	0.65	0.0	0.0	0.0
3	0.0	0.65	0.0	0.0	0.0
4	0.0	0.7	0.0	0.0	0.0
5	0.0	0.75	0.0	0.0	0.0
6	0.0	0.9	0.0	0.0	0.0
7	0.0	1.275	0.0	0.0	0.0
8	0.0	1.525	0.0	0.0	0.0
9	0.0	1.575	0.0	0.0	0.0
10	0.0	1.725	0.0	0.0	0.0
11	0.0	1.875	0.0	0.0	0.0
12	0.0	2.2	0.0	0.0	0.0
13	0.0	2.65	0.0	0.0	0.0
14	0.0	2.85	0.0	0.0	0.0
15	0.0	3.325	0.0	0.0	0.0
16	0.0	4.55	0.0	0.0	0.0
17	0.0	5.325	0.0	0.0	0.0
18	0.0	6.275	0.0	0.0	0.0
19	0.0	6.925	0.0	0.0	0.0
20	0.0	7.9	0.0	0.0	0.0
21	0.0	13.3	0.0	0.0	0.0
22	0.0	23.2	0.0	0.0	0.0
23	0.0	50.7	0.0	0.0	0.0
24	0.0	62.6	0.0	0.0	0.0
25	0.0	68.6	0.0	0.0	0.0
26	0.0	69.95	0.0	0.0	0.0
27	0.0	70.65	0.0	0.0	0.0
28	0.0	71.3	0.0	0.0	0.0
29	0.0	72.3	0.0	0.0	0.0
30	0.0	73.025	0.0	0.0	0.0
31	0.0	74.0	0.0	0.0	0.0
32	0.0	75.025	0.0	0.0	0.0
33	0.0	76.1	0.0	0.0	0.0
34	0.0	80.125	0.0	0.0	0.0
35	0.0	82.0	0.0	0.0	0.0
36	0.0	83.025	0.0	0.0	0.0
37	0.0	83.675	0.0	0.0	0.0
38	0.0	84.5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGTCG	20	6.952051E-4	44.000004	11
GGCTTGG	20	6.952051E-4	44.000004	19
CTGTCGG	20	6.952051E-4	44.000004	12
GTCGGTG	20	6.952051E-4	44.000004	14
TGTCGGT	20	6.952051E-4	44.000004	13
ACTGTTG	50	1.4551915E-11	44.0	18
GTTGAAG	50	1.4551915E-11	44.0	10
CAGTTGA	50	1.4551915E-11	44.0	8
CTGAGCT	25	3.6958358E-5	44.0	11
CCAGTTG	50	1.4551915E-11	44.0	7
AACTGTT	50	1.4551915E-11	44.0	17
GAGCTTG	25	3.6958358E-5	44.0	13
GGATCCG	25	3.6958358E-5	44.0	3
CCGTCTG	25	3.6958358E-5	44.0	7
TCGGATC	25	3.6958358E-5	44.0	1
CGGATCC	25	3.6958358E-5	44.0	2
GAAGAAC	50	1.4551915E-11	44.0	13
AGAACTG	50	1.4551915E-11	44.0	15
GCTGCCA	50	1.4551915E-11	44.0	3
GAACTGT	50	1.4551915E-11	44.0	16
>>END_MODULE
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266501 spots for SRR5031046.sra
Written 266501 spots for SRR5031046.sra
Read 266511 spots for SRR5031046.sra
Written 266511 spots for SRR5031046.sra
SRR ids: ['SRR5031046.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_df2qb830
SRR5031046.sra spots: 3198022
blocks: [[1, 266501], [266502, 533002], [533003, 799503], [799504, 1066004], [1066005, 1332505], [1332506, 1599006], [1599007, 1865507], [1865508, 2132008], [2132009, 2398509], [2398510, 2665010], [2665011, 2931511], [2931512, 3198022]]
SRR5031046 file size 553224
SRR5031046 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031046 SRR5031046_1.fastq
Input file:	SRR5031046_1.fastq
trimmed:	SRR5031046-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:13:00 2020 >> started

Wed Mar  4 07:13:02 2020 >> done (1.952s)
3198022 reads processed; of these:
    261 ( 0.01%) short reads filtered out after trimming by size control
      7 ( 0.00%) empty reads filtered out after trimming by size control
3197754 (99.99%) reads available; of these:
 178010 ( 5.57%) trimmed reads available after processing
3019744 (94.43%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     78	  0.00%
 19	     93	  0.00%
 20	    185	  0.01%
 21	    225	  0.01%
 22	    453	  0.01%
 23	    532	  0.02%
 24	    579	  0.02%
 25	    792	  0.02%
 26	    607	  0.02%
 27	    817	  0.03%
 28	    877	  0.03%
 29	   1354	  0.04%
 30	   1032	  0.03%
 31	   1031	  0.03%
 32	   1234	  0.04%
 33	   1445	  0.05%
 34	   1619	  0.05%
 35	   2947	  0.09%
 36	   2687	  0.08%
 37	   3056	  0.10%
 38	   3203	  0.10%
 39	   3581	  0.11%
 40	   4175	  0.13%
 41	   3849	  0.12%
 42	   5111	  0.16%
 43	   7771	  0.24%
 44	  10592	  0.33%
 45	  13345	  0.42%
 46	  19007	  0.59%
 47	  22317	  0.70%
 48	  30871	  0.97%
 49	  32545	  1.02%
 50	3019744	 94.43%
3197754 reads passed initial QC


criterion=sequence-density
sequence-density=83.54
sequence-density-rank=1
fanout-score=21.87
fanout-score-rank=1
prefix-density=85.92
prefix-fanout=21.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC


criterion=fanout-score
sequence-density=83.54
sequence-density-rank=1
fanout-score=21.87
fanout-score-rank=1
prefix-density=85.92
prefix-fanout=21.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC -o SRR5031046 -
Input file:	STDIN
trimmed:	SRR5031046-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:13:18 2020 >> started

Wed Mar  4 07:13:23 2020 >> done (4.949s)
3121617 reads processed; of these:
 192444 ( 6.16%) short reads filtered out after trimming by size control
  21785 ( 0.70%) empty reads filtered out after trimming by size control
2907388 (93.14%) reads available; of these:
2721746 (93.61%) trimmed reads available after processing
 185642 ( 6.39%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16935	  0.58%
 19	  33333	  1.15%
 20	 154669	  5.32%
 21	 320381	 11.02%
 22	 859098	 29.55%
 23	 379217	 13.04%
 24	 171534	  5.90%
 25	  49671	  1.71%
 26	  26537	  0.91%
 27	  21907	  0.75%
 28	  26034	  0.90%
 29	  20099	  0.69%
 30	  32756	  1.13%
 31	  39118	  1.35%
 32	  47995	  1.65%
 33	 135319	  4.65%
 34	  43182	  1.49%
 35	  29958	  1.03%
 36	  33750	  1.16%
 37	  19920	  0.69%
 38	  20695	  0.71%
 39	  19586	  0.67%
 40	  31076	  1.07%
 41	  38472	  1.32%
 42	  42187	  1.45%
 43	  29164	  1.00%
 44	  22135	  0.76%
 45	  33963	  1.17%
 46	  25386	  0.87%
 47	  17829	  0.61%
 48	   1809	  0.06%
 49	   2708	  0.09%
 50	 160965	  5.54%


criterion=sequence-density
sequence-density=6.30
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.59
sequence-density-rank=23
fanout-score=13.08
fanout-score-rank=1
prefix-density=7.70
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 07:13:46
                             Started mapping on |	Mar 04 07:13:46
                                    Finished on |	Mar 04 07:14:11
       Mapping speed, Million of reads per hour |	429.63

                          Number of input reads |	2983525
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1561105
                        Uniquely mapped reads % |	52.32%
                          Average mapped length |	25.39
                       Number of splices: Total |	2347
            Number of splices: Annotated (sjdb) |	526
                       Number of splices: GT/AG |	2206
                       Number of splices: GC/AG |	41
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	100
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.76
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	941773
             % of reads mapped to multiple loci |	31.57%
        Number of reads mapped to too many loci |	76199
             % of reads mapped to too many loci |	2.55%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.90%
                     % of reads unmapped: other |	0.65%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	480647	480647	480647
N_multimapping	941773	941773	941773
N_noFeature	78036	100897	1081792
N_ambiguous	864142	459405	15866
UnstrandedReadsAssigned:618927 PositiveStrandReadsAssigned:1000803 NegativeStrandReadsAssigned:463447
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031046 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031046-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,983,525 reads, 728,463 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,157 rounds

 109283 SRR5031046.ke.tsv
  52636 SRR5031046.se.tsv
 161919 total
==> SRR5031046.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031046.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031046 completed mapping pipeline successfully
