Starting /dee2/code/volunteer_pipeline.sh SRR5030992
    current disk space = 160573997056
    free memory = 1046785292 
SRR5030992 SRAfilesize
6874338b7557cfa095149298ddd35aac  SRR5030992.sra
SRR5030992.sra file validated
SRR5030992 is single end
SRR5030992 is conventional basespace
SRR5030992 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36325	33.0	31.0	34.0	31.0	34.0
2	32.574	34.0	31.0	34.0	31.0	34.0
3	32.605	34.0	31.0	34.0	31.0	34.0
4	36.16	37.0	35.0	37.0	35.0	37.0
5	36.05275	37.0	35.0	37.0	35.0	37.0
6	35.99	37.0	35.0	37.0	35.0	37.0
7	35.93125	37.0	35.0	37.0	35.0	37.0
8	36.019	37.0	35.0	37.0	35.0	37.0
9	37.646	39.0	37.0	39.0	35.0	39.0
10	37.57825	39.0	37.0	39.0	35.0	39.0
11	37.14325	39.0	37.0	39.0	33.0	39.0
12	37.55425	39.0	37.0	39.0	35.0	39.0
13	37.59825	39.0	37.0	39.0	35.0	39.0
14	39.03825	40.0	38.0	41.0	35.0	41.0
15	38.9135	40.0	38.0	41.0	35.0	41.0
16	38.92125	40.0	38.0	41.0	35.0	41.0
17	39.09875	40.0	38.0	41.0	36.0	41.0
18	38.9225	41.0	38.0	41.0	35.0	41.0
19	38.81075	40.0	38.0	41.0	35.0	41.0
20	38.659	40.0	38.0	41.0	34.0	41.0
21	38.878	40.0	38.0	41.0	35.0	41.0
22	38.68325	40.0	38.0	41.0	34.0	41.0
23	38.54925	40.0	38.0	41.0	34.0	41.0
24	38.79975	40.0	38.0	41.0	35.0	41.0
25	38.93275	40.0	38.0	41.0	35.0	41.0
26	38.838	40.0	38.0	41.0	35.0	41.0
27	38.9455	40.0	39.0	41.0	35.0	41.0
28	38.781	40.0	38.0	41.0	34.0	41.0
29	38.3395	40.0	38.0	41.0	33.0	41.0
30	38.31075	40.0	38.0	41.0	33.0	41.0
31	38.487	40.0	38.0	41.0	34.0	41.0
32	38.5655	40.0	38.0	41.0	34.0	41.0
33	38.21675	40.0	38.0	41.0	33.0	41.0
34	38.144	40.0	38.0	41.0	33.0	41.0
35	38.00975	40.0	38.0	41.0	33.0	41.0
36	37.95525	40.0	37.0	41.0	33.0	41.0
37	37.2105	39.0	37.0	41.0	31.0	41.0
38	37.299	39.0	36.0	41.0	31.0	41.0
39	37.7055	40.0	37.0	41.0	33.0	41.0
40	37.79625	40.0	37.0	41.0	33.0	41.0
41	38.00275	40.0	37.0	41.0	33.0	41.0
42	38.0025	40.0	37.0	41.0	33.0	41.0
43	37.615	40.0	37.0	41.0	32.0	41.0
44	37.79825	40.0	37.0	41.0	33.0	41.0
45	37.79725	40.0	37.0	41.0	33.0	41.0
46	37.612	40.0	37.0	41.0	33.0	41.0
47	36.82975	40.0	36.0	41.0	31.0	41.0
48	36.722	39.0	35.0	41.0	31.0	41.0
49	36.723	39.0	35.0	41.0	31.0	41.0
50	36.10825	38.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	3.0
20	2.0
21	1.0
22	4.0
23	3.0
24	6.0
25	9.0
26	12.0
27	27.0
28	20.0
29	45.0
30	46.0
31	55.0
32	91.0
33	109.0
34	168.0
35	204.0
36	287.0
37	422.0
38	752.0
39	1729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.899999999999999	25.15	45.5	17.45
2	22.60978670012547	26.72521957340025	20.903387703889585	29.76160602258469
3	24.125	16.35	24.625	34.9
4	36.125	28.799999999999997	14.35	20.724999999999998
5	19.875	37.425000000000004	28.275	14.424999999999999
6	28.549999999999997	17.275	37.175000000000004	17.0
7	19.225	19.725	19.775000000000002	41.275
8	21.55	28.275	20.175	30.0
9	24.4	31.574999999999996	24.8	19.225
10	21.75	20.5	29.5	28.249999999999996
11	31.900000000000002	12.625	17.599999999999998	37.875
12	16.875	20.549999999999997	30.975	31.6
13	28.549999999999997	16.8	33.725	20.925
14	29.5	29.549999999999997	20.424999999999997	20.525
15	35.099999999999994	22.675	27.0	15.225
16	19.400000000000002	18.825	25.0	36.775000000000006
17	27.675	23.175	34.699999999999996	14.45
18	28.050000000000004	15.049999999999999	29.825000000000003	27.075
19	36.275	13.425	32.875	17.424999999999997
20	40.9	16.725	25.825	16.55
21	25.3	23.599999999999998	22.525000000000002	28.575
22	31.724999999999998	15.125	41.8	11.35
23	25.75	14.274999999999999	40.925	19.05
24	47.349999999999994	15.825	27.450000000000003	9.375
25	46.949999999999996	23.974999999999998	16.575	12.5
26	29.025000000000002	42.075	15.15	13.750000000000002
27	17.95	43.025000000000006	22.525000000000002	16.5
28	14.75	24.224999999999998	43.225	17.8
29	14.85	14.6	51.5	19.05
30	12.675	7.6499999999999995	33.625	46.050000000000004
31	15.299999999999999	6.4750000000000005	45.45	32.775
32	28.575	6.825	22.45	42.15
33	49.425000000000004	11.75	15.299999999999999	23.525
34	55.00000000000001	8.774999999999999	18.4	17.825
35	62.625	8.575000000000001	18.525	10.274999999999999
36	42.199999999999996	9.700000000000001	34.35	13.750000000000002
37	42.725	13.4	20.8	23.075000000000003
38	24.075	20.175	14.625	41.125
39	17.150000000000002	22.875	12.825000000000001	47.15
40	17.025000000000002	43.375	14.975	24.625
41	23.3	44.574999999999996	11.175	20.95
42	44.025	28.525	15.65	11.799999999999999
43	49.0	27.675	9.675	13.65
44	33.575	41.349999999999994	9.65	15.425
45	20.674999999999997	45.85	15.925	17.549999999999997
46	17.175	24.7	17.75	40.375
47	11.85	15.85	38.550000000000004	33.75
48	14.174999999999999	12.3	21.175	52.349999999999994
49	14.299999999999999	18.15	16.075	51.475
50	17.775	34.175	15.6	32.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	1.0
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	2.5
28	4.0
29	4.5
30	5.0
31	10.5
32	16.0
33	15.5
34	15.0
35	26.5
36	38.0
37	41.0
38	44.0
39	46.5
40	49.0
41	56.0
42	63.0
43	73.5
44	84.0
45	129.0
46	174.0
47	280.5
48	387.0
49	505.0
50	623.0
51	731.5
52	840.0
53	606.5
54	373.0
55	452.0
56	531.0
57	391.0
58	251.0
59	163.5
60	76.0
61	61.5
62	47.0
63	113.5
64	180.0
65	109.5
66	39.0
67	29.5
68	20.0
69	28.5
70	37.0
71	65.0
72	93.0
73	49.0
74	5.0
75	2.5
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.375
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97765363128491	30.599999999999998
2	9.497206703910614	7.6499999999999995
3	4.0968342644320295	4.95
4	2.4829298572315333	4.0
5	1.675977653631285	3.375
6	1.2414649286157666	3.0
7	0.9931719428926132	2.8000000000000003
8	0.5586592178770949	1.7999999999999998
9	0.37243947858473	1.35
>10	2.60707635009311	19.900000000000002
>50	0.2482929857231533	8.15
>100	0.2482929857231533	12.425
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	136	3.4000000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	122	3.05	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	121	3.025	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	118	2.9499999999999997	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	91	2.275	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	84	2.1	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	82	2.0500000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	69	1.725	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	48	1.2	RNA PCR Primer, Index 1 (97% over 35bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	40	1.0	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	31	0.775	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	27	0.675	RNA PCR Primer, Index 1 (100% over 33bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC	27	0.675	RNA PCR Primer, Index 12 (100% over 44bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	25	0.625	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	20	0.5	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	20	0.5	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	17	0.42500000000000004	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	13	0.325	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	12	0.3	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	11	0.27499999999999997	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	11	0.27499999999999997	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGCATTCTCGGGTGCC	10	0.25	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	9	0.22499999999999998	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	8	0.2	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	7	0.17500000000000002	RNA PCR Primer, Index 2 (100% over 34bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 12 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT	6	0.15	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ATGACATAAAATTCATGCTCAATAGGATTACGCTGAGGCCCTGGAATTCT	6	0.15	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTGGAATTCTCGGGTG	6	0.15	No Hit
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCCAGGAACTCCAGTCACCT	5	0.125	RNA PCR Primer, Index 12 (97% over 35bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	5	0.125	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTGAAGGTCGTGAGTTCGTTCCTCACACGGGGCACCATGGAATTCTCGG	5	0.125	No Hit
AGAATTGTGGCTGGACATCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	5	0.125	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC	5	0.125	No Hit
CTGCGTTCGCGCTCTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.2	0.0	0.0	0.0
2	0.0	0.2	0.0	0.0	0.0
3	0.0	0.275	0.0	0.0	0.0
4	0.0	0.35	0.0	0.0	0.0
5	0.0	0.425	0.0	0.0	0.0
6	0.0	0.475	0.0	0.0	0.0
7	0.0	1.225	0.0	0.0	0.0
8	0.0	1.4	0.0	0.0	0.0
9	0.0	1.5	0.0	0.0	0.0
10	0.0	1.575	0.0	0.0	0.0
11	0.0	1.95	0.0	0.0	0.0
12	0.0	2.175	0.0	0.0	0.0
13	0.0	2.375	0.0	0.0	0.0
14	0.0	2.7	0.0	0.0	0.0
15	0.0	2.9	0.0	0.0	0.0
16	0.0	4.85	0.0	0.0	0.0
17	0.0	5.475	0.0	0.0	0.0
18	0.0	6.925	0.0	0.0	0.0
19	0.0	7.575	0.0	0.0	0.0
20	0.0	8.65	0.0	0.0	0.0
21	0.0	13.7	0.0	0.0	0.0
22	0.0	22.925	0.0	0.0	0.0
23	0.0	48.375	0.0	0.0	0.0
24	0.0	60.25	0.0	0.0	0.0
25	0.0	66.4	0.0	0.0	0.0
26	0.0	67.775	0.0	0.0	0.0
27	0.0	68.425	0.0	0.0	0.0
28	0.0	69.15	0.0	0.0	0.0
29	0.0	69.9	0.0	0.0	0.0
30	0.0	70.725	0.0	0.0	0.0
31	0.0	71.525	0.0	0.0	0.0
32	0.0	73.075	0.0	0.0	0.0
33	0.0	74.425	0.0	0.0	0.0
34	0.0	79.2	0.0	0.0	0.0
35	0.0	80.675	0.0	0.0	0.0
36	0.0	81.85	0.0	0.0	0.0
37	0.0	83.425	0.0	0.0	0.0
38	0.0	84.125	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGAGT	20	6.952051E-4	44.000004	17
GAGCTTG	65	0.0	44.000004	13
GGATCCG	65	0.0	44.000004	3
CCGTCTG	65	0.0	44.000004	7
TCGGATC	65	0.0	44.000004	1
CGGATCC	65	0.0	44.000004	2
CTTGGCT	40	5.315087E-9	44.000004	16
GATCCGT	65	0.0	44.000004	4
GGCTTGG	40	5.315087E-9	44.000004	19
CGGTGAG	20	6.952051E-4	44.000004	16
TCCGTCT	65	0.0	44.000004	6
ATCCGTC	65	0.0	44.000004	5
TGAGCTT	65	0.0	44.000004	12
CGTCTGA	65	0.0	44.000004	8
AACGCTG	25	3.6958358E-5	44.0	8
ACGCTGT	25	3.6958358E-5	44.0	9
TTCAACG	25	3.6958358E-5	44.0	5
TCGGTGA	25	3.6958358E-5	44.0	15
CATTCAA	25	3.6958358E-5	44.0	3
TCAACGC	25	3.6958358E-5	44.0	6
>>END_MODULE
Read 80099 spots for SRR5030992.sra
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SRR ids: ['SRR5030992.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_2_r0fvq9
SRR5030992.sra spots: 3844768
blocks: [[1, 80099], [80100, 160198], [160199, 240297], [240298, 320396], [320397, 400495], [400496, 480594], [480595, 560693], [560694, 640792], [640793, 720891], [720892, 800990], [800991, 881089], [881090, 961188], [961189, 1041287], [1041288, 1121386], [1121387, 1201485], [1201486, 1281584], [1281585, 1361683], [1361684, 1441782], [1441783, 1521881], [1521882, 1601980], [1601981, 1682079], [1682080, 1762178], [1762179, 1842277], [1842278, 1922376], [1922377, 2002475], [2002476, 2082574], [2082575, 2162673], [2162674, 2242772], [2242773, 2322871], [2322872, 2402970], [2402971, 2483069], [2483070, 2563168], [2563169, 2643267], [2643268, 2723366], [2723367, 2803465], [2803466, 2883564], [2883565, 2963663], [2963664, 3043762], [3043763, 3123861], [3123862, 3203960], [3203961, 3284059], [3284060, 3364158], [3364159, 3444257], [3444258, 3524356], [3524357, 3604455], [3604456, 3684554], [3684555, 3764653], [3764654, 3844768]]
SRR5030992 file size 665321
SRR5030992 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR5030992 SRR5030992_1.fastq
Input file:	SRR5030992_1.fastq
trimmed:	SRR5030992-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Wed Mar  4 07:01:02 2020 >> started

Wed Mar  4 07:01:04 2020 >> done (2.184s)
3844768 reads processed; of these:
    353 ( 0.01%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
3844410 (99.99%) reads available; of these:
 234232 ( 6.09%) trimmed reads available after processing
3610178 (93.91%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     93	  0.00%
 19	    132	  0.00%
 20	    264	  0.01%
 21	    258	  0.01%
 22	    501	  0.01%
 23	    546	  0.01%
 24	    604	  0.02%
 25	    853	  0.02%
 26	    701	  0.02%
 27	    884	  0.02%
 28	    986	  0.03%
 29	   1669	  0.04%
 30	   1097	  0.03%
 31	   1179	  0.03%
 32	   1497	  0.04%
 33	   1764	  0.05%
 34	   2006	  0.05%
 35	   3744	  0.10%
 36	   3407	  0.09%
 37	   4181	  0.11%
 38	   3975	  0.10%
 39	   4319	  0.11%
 40	   4432	  0.12%
 41	   4936	  0.13%
 42	   6123	  0.16%
 43	   9024	  0.23%
 44	  12059	  0.31%
 45	  15702	  0.41%
 46	  23944	  0.62%
 47	  27429	  0.71%
 48	  41316	  1.07%
 49	  54607	  1.42%
 50	3610178	 93.91%
3844410 reads passed initial QC


criterion=sequence-density
sequence-density=83.45
sequence-density-rank=1
fanout-score=21.20
fanout-score-rank=1
prefix-density=86.09
prefix-fanout=20.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC


criterion=fanout-score
sequence-density=83.45
sequence-density-rank=1
fanout-score=21.20
fanout-score-rank=1
prefix-density=86.09
prefix-fanout=20.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC -o SRR5030992 -
Input file:	STDIN
trimmed:	SRR5030992-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Wed Mar  4 07:01:22 2020 >> started

Wed Mar  4 07:01:28 2020 >> done (5.600s)
3752877 reads processed; of these:
 269657 ( 7.19%) short reads filtered out after trimming by size control
  13513 ( 0.36%) empty reads filtered out after trimming by size control
3469707 (92.45%) reads available; of these:
3262693 (94.03%) trimmed reads available after processing
 207014 ( 5.97%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  20969	  0.60%
 19	  39029	  1.12%
 20	 191755	  5.53%
 21	 356870	 10.29%
 22	 937507	 27.02%
 23	 430465	 12.41%
 24	 218528	  6.30%
 25	  63399	  1.83%
 26	  34094	  0.98%
 27	  28771	  0.83%
 28	  29943	  0.86%
 29	  23139	  0.67%
 30	  35235	  1.02%
 31	  58793	  1.69%
 32	  49905	  1.44%
 33	 191863	  5.53%
 34	  65473	  1.89%
 35	  52360	  1.51%
 36	  62889	  1.81%
 37	  28895	  0.83%
 38	  31591	  0.91%
 39	  27323	  0.79%
 40	  36674	  1.06%
 41	  46926	  1.35%
 42	  53995	  1.56%
 43	  39144	  1.13%
 44	  31900	  0.92%
 45	  43538	  1.25%
 46	  32901	  0.95%
 47	  24636	  0.71%
 48	   2306	  0.07%
 49	   3717	  0.11%
 50	 175174	  5.05%


criterion=sequence-density
sequence-density=7.14
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.71
sequence-density-rank=21
fanout-score=11.48
fanout-score-rank=1
prefix-density=8.13
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 07:02:09
                             Started mapping on |	Mar 04 07:02:10
                                    Finished on |	Mar 04 07:02:39
       Mapping speed, Million of reads per hour |	442.08

                          Number of input reads |	3561240
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1831639
                        Uniquely mapped reads % |	51.43%
                          Average mapped length |	25.25
                       Number of splices: Total |	2084
            Number of splices: Annotated (sjdb) |	373
                       Number of splices: GT/AG |	1962
                       Number of splices: GC/AG |	24
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	97
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.80
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1104352
             % of reads mapped to multiple loci |	31.01%
        Number of reads mapped to too many loci |	117799
             % of reads mapped to too many loci |	3.31%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.04%
                     % of reads unmapped: other |	1.21%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	625249	625249	625249
N_multimapping	1104352	1104352	1104352
N_noFeature	107721	135376	1211480
N_ambiguous	983723	434926	17634
UnstrandedReadsAssigned:740195 PositiveStrandReadsAssigned:1261337 NegativeStrandReadsAssigned:602525
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030992 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030992-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,561,240 reads, 868,846 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,198 rounds

 109283 SRR5030992.ke.tsv
  52636 SRR5030992.se.tsv
 161919 total
==> SRR5030992.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030992.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	1
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030992 completed mapping pipeline successfully
