Starting /dee2/code/volunteer_pipeline.sh SRR5030877
    current disk space = 160665878528
    free memory = 73928904 
SRR5030877 SRAfilesize
b58f6c8fec9b4d3141ced6234c5e81a3  SRR5030877.sra
SRR5030877.sra file validated
SRR5030877 is single end
SRR5030877 is conventional basespace
SRR5030877 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.461	34.0	31.0	34.0	31.0	34.0
2	32.74675	34.0	31.0	34.0	31.0	34.0
3	32.7335	34.0	31.0	34.0	31.0	34.0
4	36.25175	37.0	37.0	37.0	35.0	37.0
5	36.334	37.0	37.0	37.0	35.0	37.0
6	36.125	37.0	36.0	37.0	35.0	37.0
7	36.12525	37.0	36.0	37.0	35.0	37.0
8	36.1775	37.0	36.0	37.0	35.0	37.0
9	37.93575	39.0	38.0	39.0	35.0	39.0
10	37.91775	39.0	38.0	39.0	35.0	39.0
11	37.89125	39.0	38.0	39.0	35.0	39.0
12	37.70375	39.0	38.0	39.0	35.0	39.0
13	37.80225	39.0	38.0	39.0	35.0	39.0
14	39.2825	40.0	39.0	41.0	36.0	41.0
15	39.19875	40.0	39.0	41.0	36.0	41.0
16	39.16675	40.0	38.0	41.0	36.0	41.0
17	39.14875	40.0	39.0	41.0	36.0	41.0
18	39.3015	41.0	39.0	41.0	36.0	41.0
19	39.294	41.0	39.0	41.0	36.0	41.0
20	39.14825	40.0	39.0	41.0	36.0	41.0
21	39.122	40.0	39.0	41.0	36.0	41.0
22	39.2565	40.0	39.0	41.0	36.0	41.0
23	39.1725	41.0	39.0	41.0	36.0	41.0
24	38.36825	40.0	38.0	41.0	34.0	41.0
25	38.32675	40.0	38.0	41.0	33.0	41.0
26	38.4335	40.0	38.0	41.0	34.0	41.0
27	38.60775	40.0	38.0	41.0	34.0	41.0
28	38.47225	40.0	38.0	41.0	34.0	41.0
29	37.99025	40.0	38.0	41.0	32.0	41.0
30	38.1525	40.0	38.0	41.0	33.0	41.0
31	38.691	40.0	38.0	41.0	35.0	41.0
32	38.73	40.0	38.0	41.0	35.0	41.0
33	37.5075	40.0	37.0	41.0	31.0	41.0
34	37.65975	40.0	37.0	41.0	31.0	41.0
35	37.48325	40.0	37.0	41.0	32.0	41.0
36	33.3295	38.0	30.0	40.0	18.0	41.0
37	35.6485	38.0	34.0	40.0	30.0	41.0
38	36.9205	39.0	37.0	40.0	31.0	41.0
39	37.1875	39.0	37.0	40.0	32.0	41.0
40	37.216	39.0	37.0	40.0	32.0	41.0
41	36.86775	39.0	36.0	40.0	31.0	41.0
42	36.9175	39.0	36.0	41.0	31.0	41.0
43	36.9925	39.0	36.0	41.0	31.0	41.0
44	37.14375	40.0	37.0	41.0	32.0	41.0
45	36.772	39.0	36.0	41.0	31.0	41.0
46	34.38225	38.0	33.0	40.0	24.0	41.0
47	35.34475	38.0	34.0	40.0	27.0	41.0
48	35.70825	39.0	35.0	40.0	29.0	41.0
49	35.0985	38.0	34.0	40.0	28.0	41.0
50	31.25075	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	1.0
17	2.0
18	2.0
19	4.0
20	4.0
21	8.0
22	6.0
23	10.0
24	10.0
25	19.0
26	27.0
27	23.0
28	34.0
29	30.0
30	58.0
31	73.0
32	110.0
33	100.0
34	148.0
35	201.0
36	323.0
37	514.0
38	1072.0
39	1219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.71071071071071	24.24924924924925	47.2972972972973	17.74274274274274
2	22.375	26.1	21.525	30.0
3	23.65	19.975	21.2	35.175
4	30.15	28.9	14.05	26.900000000000002
5	20.525	41.099999999999994	24.85	13.525
6	35.225	16.650000000000002	32.475	15.65
7	17.5	21.375	25.6	35.525
8	25.874999999999996	28.625	17.325	28.175
9	21.7	34.375	27.250000000000004	16.675
10	22.825	21.65	31.525	24.0
11	32.824999999999996	12.375	16.75	38.05
12	15.7	21.4	30.025000000000002	32.875
13	28.625	21.65	29.725	20.0
14	28.599999999999998	31.924999999999997	20.849999999999998	18.625
15	33.725	26.924999999999997	24.8	14.549999999999999
16	20.8	22.075	23.400000000000002	33.725
17	23.3	24.075	37.724999999999994	14.899999999999999
18	21.425	21.25	30.049999999999997	27.275
19	37.125	14.7	29.7	18.475
20	40.9	17.549999999999997	28.000000000000004	13.55
21	25.025	18.45	26.900000000000002	29.625
22	27.700000000000003	13.5	46.525	12.275
23	28.299999999999997	13.025	42.35	16.325
24	51.849999999999994	13.725000000000001	24.05	10.375
25	46.525	25.074999999999996	14.099999999999998	14.299999999999999
26	25.6	44.725	15.35	14.325
27	17.05	44.925	22.85	15.174999999999999
28	14.424999999999999	22.0	47.425	16.150000000000002
29	13.950000000000001	13.3	53.25	19.5
30	11.525	9.2	33.425	45.85
31	15.725	7.249999999999999	45.9	31.125000000000004
32	29.875	7.925	20.625	41.575
33	51.525	13.575000000000001	13.475000000000001	21.425
34	57.65	9.325	17.4	15.625
35	59.824999999999996	8.55	19.0	12.625
36	43.725	9.45	33.15	13.675
37	43.875	12.525	19.975	23.625
38	23.35	17.299999999999997	12.575	46.775
39	15.975	24.825	12.4	46.800000000000004
40	17.424999999999997	46.125	14.45	22.0
41	24.8	45.45	11.799999999999999	17.95
42	49.525000000000006	26.150000000000002	14.174999999999999	10.15
43	48.375	29.849999999999998	9.775	12.0
44	28.999999999999996	48.625	9.725	12.65
45	18.8	49.8	13.05	18.35
46	15.6	25.3	19.900000000000002	39.2
47	10.9	16.45	39.15	33.5
48	13.100000000000001	13.8	19.25	53.849999999999994
49	13.775	21.525	14.35	50.349999999999994
50	19.575	36.25	14.575	29.599999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.5
26	3.0
27	3.0
28	3.0
29	3.0
30	3.0
31	13.0
32	23.0
33	24.5
34	26.0
35	29.0
36	32.0
37	56.5
38	81.0
39	77.5
40	74.0
41	73.5
42	73.0
43	89.0
44	105.0
45	161.0
46	217.0
47	301.5
48	386.0
49	512.0
50	638.0
51	710.5
52	783.0
53	608.0
54	433.0
55	449.0
56	465.0
57	355.0
58	245.0
59	168.0
60	91.0
61	68.0
62	45.0
63	97.0
64	149.0
65	88.5
66	28.0
67	22.0
68	16.0
69	13.5
70	11.0
71	28.5
72	46.0
73	30.0
74	14.0
75	7.5
76	1.0
77	0.5
78	0.0
79	2.0
80	4.0
81	3.5
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.20584498094028	29.2
2	10.101651842439646	7.95
3	4.129606099110546	4.875
4	2.41423125794155	3.8
5	1.6518424396442184	3.25
6	2.096569250317662	4.95
7	0.9529860228716646	2.625
8	0.6353240152477764	2.0
9	0.3176620076238882	1.125
>10	2.9860228716645487	22.650000000000002
>50	0.3176620076238882	8.649999999999999
>100	0.1905972045743329	8.924999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	129	3.225	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	122	3.05	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	106	2.65	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	95	2.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	71	1.775	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	57	1.425	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	53	1.325	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	45	1.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	44	1.0999999999999999	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	30	0.75	RNA PCR Primer, Index 7 (100% over 35bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	27	0.675	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	24	0.6	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	19	0.475	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	19	0.475	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	18	0.44999999999999996	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	16	0.4	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	15	0.375	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC	13	0.325	RNA PCR Primer, Index 7 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	9	0.22499999999999998	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	8	0.2	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	7	0.17500000000000002	RNA PCR Primer, Index 2 (100% over 34bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 7 (100% over 50bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	7	0.17500000000000002	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	6	0.15	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATGGAAT	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	6	0.15	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATT	6	0.15	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	6	0.15	No Hit
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	5	0.125	RNA PCR Primer, Index 7 (100% over 35bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG	5	0.125	RNA PCR Primer, Index 7 (100% over 36bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGCC	5	0.125	No Hit
GGTCCATGATGAAGCCCATTATCAGTGGACATCTATGGATGAGAAATGCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.25	0.0	0.0	0.0
3	0.0	0.35	0.0	0.0	0.0
4	0.0	0.35	0.0	0.0	0.0
5	0.0	0.375	0.0	0.0	0.0
6	0.0	0.375	0.0	0.0	0.0
7	0.0	0.75	0.0	0.0	0.0
8	0.0	0.9	0.0	0.0	0.0
9	0.0	1.0	0.0	0.0	0.0
10	0.0	1.125	0.0	0.0	0.0
11	0.0	1.225	0.0	0.0	0.0
12	0.0	1.5	0.0	0.0	0.0
13	0.0	1.675	0.0	0.0	0.0
14	0.0	1.85	0.0	0.0	0.0
15	0.0	2.2	0.0	0.0	0.0
16	0.0	3.4	0.0	0.0	0.0
17	0.0	4.125	0.0	0.0	0.0
18	0.0	5.225	0.0	0.0	0.0
19	0.0	5.575	0.0	0.0	0.0
20	0.0	6.45	0.0	0.0	0.0
21	0.0	10.85	0.0	0.0	0.0
22	0.0	21.75	0.0	0.0	0.0
23	0.0	49.275	0.0	0.0	0.0
24	0.0	59.875	0.0	0.0	0.0
25	0.0	64.425	0.0	0.0	0.0
26	0.0	65.925	0.0	0.0	0.0
27	0.0	66.425	0.0	0.0	0.0
28	0.0	67.125	0.0	0.0	0.0
29	0.0	67.625	0.0	0.0	0.0
30	0.0	67.9	0.0	0.0	0.0
31	0.0	68.575	0.0	0.0	0.0
32	0.0	69.325	0.0	0.0	0.0
33	0.0	70.0	0.0	0.0	0.0
34	0.0	73.825	0.0	0.0	0.0
35	0.0	74.95	0.0	0.0	0.0
36	0.0	75.775	0.0	0.0	0.0
37	0.0	76.7	0.0	0.0	0.0
38	0.0	77.25	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
ACAGTGA	20	6.952051E-4	44.000004	3
CTGAGAC	20	6.952051E-4	44.000004	4
CCTGAGA	20	6.952051E-4	44.000004	3
CTTGGCT	20	6.952051E-4	44.000004	16
GGCTTGG	40	5.315087E-9	44.000004	19
CCCTGAG	20	6.952051E-4	44.000004	2
AGACCCT	20	6.952051E-4	44.000004	7
GAGACCC	20	6.952051E-4	44.000004	6
TCCCTGA	20	6.952051E-4	44.000004	1
AAGTAAT	35	1.02281774E-7	44.0	4
TCAAGTA	35	1.02281774E-7	44.0	2
ATCCAGG	35	1.02281774E-7	44.0	9
TTCAAGT	35	1.02281774E-7	44.0	1
AGGATAG	35	1.02281774E-7	44.0	13
ATAGGCT	35	1.02281774E-7	44.0	16
GAGCTTG	30	1.9515464E-6	44.0	13
GGATCCG	30	1.9515464E-6	44.0	3
CCGTCTG	30	1.9515464E-6	44.0	7
TCGGATC	30	1.9515464E-6	44.0	1
>>END_MODULE
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
Read 303224 spots for SRR5030877.sra
Written 303224 spots for SRR5030877.sra
Read 303222 spots for SRR5030877.sra
Written 303222 spots for SRR5030877.sra
SRR ids: ['SRR5030877.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_jybsbt65
SRR5030877.sra spots: 3638666
blocks: [[1, 303222], [303223, 606444], [606445, 909666], [909667, 1212888], [1212889, 1516110], [1516111, 1819332], [1819333, 2122554], [2122555, 2425776], [2425777, 2728998], [2728999, 3032220], [3032221, 3335442], [3335443, 3638666]]
SRR5030877 file size 629585
SRR5030877 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030877 SRR5030877_1.fastq
Input file:	SRR5030877_1.fastq
trimmed:	SRR5030877-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:34:24 2020 >> started

Wed Mar  4 06:34:27 2020 >> done (2.202s)
3638666 reads processed; of these:
    203 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
3638459 (99.99%) reads available; of these:
 266072 ( 7.31%) trimmed reads available after processing
3372387 (92.69%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     57	  0.00%
 19	     98	  0.00%
 20	    202	  0.01%
 21	    257	  0.01%
 22	    411	  0.01%
 23	    509	  0.01%
 24	    505	  0.01%
 25	    625	  0.02%
 26	    526	  0.01%
 27	    614	  0.02%
 28	    836	  0.02%
 29	   1191	  0.03%
 30	    963	  0.03%
 31	   1273	  0.03%
 32	   1732	  0.05%
 33	   2693	  0.07%
 34	   7823	  0.22%
 35	   4107	  0.11%
 36	   3225	  0.09%
 37	   3305	  0.09%
 38	   4598	  0.13%
 39	   4467	  0.12%
 40	   6743	  0.19%
 41	   5215	  0.14%
 42	   8468	  0.23%
 43	   6915	  0.19%
 44	  18850	  0.52%
 45	  19023	  0.52%
 46	  17874	  0.49%
 47	  20733	  0.57%
 48	  51788	  1.42%
 49	  70446	  1.94%
 50	3372387	 92.69%
3638459 reads passed initial QC


criterion=sequence-density
sequence-density=76.46
sequence-density-rank=1
fanout-score=20.41
fanout-score-rank=2
prefix-density=78.74
prefix-fanout=19.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC


criterion=fanout-score
sequence-density=1.68
sequence-density-rank=9
fanout-score=143.81
fanout-score-rank=1
prefix-density=78.54
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC -o SRR5030877 -
Input file:	STDIN
trimmed:	SRR5030877-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:34:43 2020 >> started

Wed Mar  4 06:34:49 2020 >> done (5.468s)
3543954 reads processed; of these:
 167247 ( 4.72%) short reads filtered out after trimming by size control
  10623 ( 0.30%) empty reads filtered out after trimming by size control
3366084 (94.98%) reads available; of these:
2885232 (85.71%) trimmed reads available after processing
 480852 (14.29%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16487	  0.49%
 19	  29640	  0.88%
 20	 163957	  4.87%
 21	 372860	 11.08%
 22	 950641	 28.24%
 23	 399759	 11.88%
 24	 175182	  5.20%
 25	  47077	  1.40%
 26	  23225	  0.69%
 27	  19484	  0.58%
 28	  20851	  0.62%
 29	  15898	  0.47%
 30	  23555	  0.70%
 31	  27763	  0.82%
 32	  32877	  0.98%
 33	 140181	  4.16%
 34	  44044	  1.31%
 35	  34703	  1.03%
 36	  45170	  1.34%
 37	  19740	  0.59%
 38	  20828	  0.62%
 39	  21787	  0.65%
 40	  32395	  0.96%
 41	  40306	  1.20%
 42	  46741	  1.39%
 43	  34782	  1.03%
 44	  30212	  0.90%
 45	  36003	  1.07%
 46	  30598	  0.91%
 47	  26751	  0.79%
 48	   5973	  0.18%
 49	  16016	  0.48%
 50	 420598	 12.50%


criterion=sequence-density
sequence-density=5.06
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=20
fanout-score=12.97
fanout-score-rank=1
prefix-density=7.03
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:35:12
                             Started mapping on |	Mar 04 06:35:12
                                    Finished on |	Mar 04 06:35:37
       Mapping speed, Million of reads per hour |	498.32

                          Number of input reads |	3460589
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1883044
                        Uniquely mapped reads % |	54.41%
                          Average mapped length |	28.53
                       Number of splices: Total |	1361
            Number of splices: Annotated (sjdb) |	276
                       Number of splices: GT/AG |	1205
                       Number of splices: GC/AG |	25
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	129
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.47
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.11
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1063544
             % of reads mapped to multiple loci |	30.73%
        Number of reads mapped to too many loci |	111172
             % of reads mapped to too many loci |	3.21%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.10%
                     % of reads unmapped: other |	0.54%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	514001	514001	514001
N_multimapping	1063544	1063544	1063544
N_noFeature	82327	106392	1362389
N_ambiguous	1038466	597884	22877
UnstrandedReadsAssigned:762251 PositiveStrandReadsAssigned:1178768 NegativeStrandReadsAssigned:497778
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030877 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030877-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,460,589 reads, 900,069 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,180 rounds

 109283 SRR5030877.ke.tsv
  52636 SRR5030877.se.tsv
 161919 total
==> SRR5030877.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030877.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030877 completed mapping pipeline successfully
