Starting /dee2/code/volunteer_pipeline.sh SRR5030858
    current disk space = 160667598848
    free memory = 73946828 
SRR5030858 SRAfilesize
7a19beb977eba7b050403dae94c9d35d  SRR5030858.sra
SRR5030858.sra file validated
SRR5030858 is single end
SRR5030858 is conventional basespace
SRR5030858 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4385	33.0	31.0	34.0	31.0	34.0
2	32.72875	34.0	31.0	34.0	31.0	34.0
3	32.69825	34.0	31.0	34.0	31.0	34.0
4	36.25075	37.0	37.0	37.0	35.0	37.0
5	36.285	37.0	37.0	37.0	35.0	37.0
6	36.0795	37.0	36.0	37.0	35.0	37.0
7	36.0845	37.0	35.0	37.0	35.0	37.0
8	36.218	37.0	36.0	37.0	35.0	37.0
9	38.00925	39.0	38.0	39.0	35.0	39.0
10	37.85875	39.0	38.0	39.0	35.0	39.0
11	37.77225	39.0	38.0	39.0	35.0	39.0
12	37.7265	39.0	38.0	39.0	35.0	39.0
13	37.74875	39.0	38.0	39.0	35.0	39.0
14	39.17775	40.0	39.0	41.0	36.0	41.0
15	39.19975	41.0	39.0	41.0	36.0	41.0
16	39.25425	40.0	39.0	41.0	36.0	41.0
17	39.22325	40.0	39.0	41.0	36.0	41.0
18	39.35375	41.0	39.0	41.0	36.0	41.0
19	39.27175	41.0	39.0	41.0	36.0	41.0
20	39.2305	41.0	39.0	41.0	36.0	41.0
21	39.13875	40.0	39.0	41.0	36.0	41.0
22	39.29575	41.0	39.0	41.0	36.0	41.0
23	39.216	41.0	39.0	41.0	36.0	41.0
24	38.34525	40.0	38.0	41.0	33.0	41.0
25	38.5695	40.0	38.0	41.0	34.0	41.0
26	38.7735	40.0	38.0	41.0	34.0	41.0
27	38.813	40.0	38.0	41.0	35.0	41.0
28	38.628	40.0	38.0	41.0	34.0	41.0
29	38.03925	40.0	38.0	41.0	33.0	41.0
30	38.26275	40.0	38.0	41.0	33.0	41.0
31	38.70625	40.0	38.0	41.0	34.0	41.0
32	38.59575	40.0	38.0	41.0	34.0	41.0
33	37.61225	40.0	38.0	41.0	32.0	41.0
34	37.27125	40.0	37.0	41.0	30.0	41.0
35	37.2665	39.0	37.0	40.0	31.0	41.0
36	33.3255	38.0	30.0	40.0	18.0	41.0
37	35.6055	38.0	34.0	40.0	30.0	41.0
38	36.9175	39.0	37.0	40.0	31.0	41.0
39	37.13925	39.0	37.0	40.0	32.0	41.0
40	37.0255	39.0	37.0	40.0	32.0	41.0
41	36.865	39.0	36.0	40.0	31.0	41.0
42	36.85075	39.0	36.0	40.0	31.0	41.0
43	36.776	39.0	36.0	40.0	31.0	41.0
44	36.9595	39.0	37.0	41.0	31.0	41.0
45	36.56125	39.0	36.0	40.0	31.0	41.0
46	34.308	38.0	33.0	40.0	24.0	41.0
47	35.57375	38.0	35.0	40.0	28.0	41.0
48	35.74025	39.0	35.0	40.0	29.0	41.0
49	34.956	38.0	34.0	40.0	27.0	41.0
50	31.13675	35.0	29.0	39.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	0.0
16	0.0
17	2.0
18	2.0
19	3.0
20	5.0
21	6.0
22	9.0
23	10.0
24	20.0
25	15.0
26	23.0
27	33.0
28	24.0
29	43.0
30	53.0
31	68.0
32	75.0
33	104.0
34	188.0
35	204.0
36	326.0
37	518.0
38	997.0
39	1269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.539944903581267	25.77009767092412	49.135987978963186	16.55396944653143
2	23.175	26.474999999999998	18.099999999999998	32.25
3	26.513256628314156	19.809904952476238	22.461230615307652	31.21560780390195
4	32.324999999999996	29.625	13.600000000000001	24.45
5	18.725	45.175	23.825	12.275
6	31.775	17.05	38.725	12.45
7	18.325	18.25	22.775000000000002	40.65
8	23.125	33.85	13.55	29.475
9	23.799999999999997	30.8	30.825000000000003	14.575
10	22.075	19.950000000000003	30.9	27.075
11	29.275000000000002	12.950000000000001	16.25	41.525
12	14.899999999999999	19.225	31.825	34.050000000000004
13	30.599999999999998	19.675	28.1	21.625
14	30.675	35.225	17.875	16.225
15	35.675000000000004	27.425	23.825	13.075000000000001
16	15.25	21.675	22.3	40.775
17	22.15	19.650000000000002	43.325	14.875
18	24.95	17.95	35.775	21.325
19	39.550000000000004	13.775	29.25	17.424999999999997
20	44.824999999999996	18.6	23.200000000000003	13.375
21	18.825	22.425	28.249999999999996	30.5
22	30.55	13.975000000000001	43.55	11.924999999999999
23	29.45	13.350000000000001	41.5	15.7
24	49.5	16.400000000000002	25.775	8.325000000000001
25	48.425000000000004	24.6	14.499999999999998	12.475
26	28.7	44.824999999999996	15.85	10.625
27	16.725	46.0	24.8	12.475
28	12.325	26.05	46.800000000000004	14.825
29	9.675	13.775	56.925000000000004	19.625
30	10.125	7.6499999999999995	36.975	45.25
31	17.549999999999997	5.8999999999999995	44.875	31.674999999999997
32	29.849999999999998	7.875	20.4	41.875
33	52.775000000000006	9.85	14.899999999999999	22.475
34	58.4	8.375	17.0	16.225
35	61.8	8.0	18.6	11.600000000000001
36	43.25	8.575000000000001	31.724999999999998	16.45
37	43.35	9.475	20.4	26.775
38	23.9	14.85	14.975	46.275
39	18.375	25.074999999999996	9.225	47.325
40	18.4	45.6	11.625	24.375
41	27.950000000000003	46.85	9.775	15.425
42	48.65	31.225	11.375	8.75
43	49.275000000000006	32.25	9.025	9.45
44	31.324999999999996	46.925	8.25	13.5
45	17.599999999999998	50.824999999999996	13.525	18.05
46	11.625	29.4	19.375	39.6
47	8.3	17.349999999999998	37.6	36.75
48	10.375	14.549999999999999	21.025	54.05
49	15.425	19.950000000000003	15.525	49.1
50	18.95473868467117	34.45861465366342	15.753938484621155	30.83270817704426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.5
20	2.0
21	1.5
22	1.0
23	1.0
24	1.0
25	0.5
26	0.0
27	1.5
28	3.0
29	2.0
30	1.0
31	20.5
32	40.0
33	33.0
34	26.0
35	32.0
36	38.0
37	46.0
38	54.0
39	56.5
40	59.0
41	54.5
42	50.0
43	82.5
44	115.0
45	190.0
46	265.0
47	321.0
48	377.0
49	490.0
50	603.0
51	700.5
52	798.0
53	611.0
54	424.0
55	494.0
56	564.0
57	411.0
58	258.0
59	160.0
60	62.0
61	52.0
62	42.0
63	58.5
64	75.0
65	49.5
66	24.0
67	17.5
68	11.0
69	20.5
70	30.0
71	41.5
72	53.0
73	33.5
74	14.0
75	8.0
76	2.0
77	1.5
78	1.0
79	2.0
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.05
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4259501965924	28.775000000000002
2	9.043250327653997	6.9
3	3.473132372214941	3.975
4	2.4246395806028835	3.6999999999999997
5	1.7693315858453473	3.375
6	1.310615989515072	3.0
7	0.7863695937090431	2.1
8	0.9829619921363041	3.0
9	0.655307994757536	2.25
>10	3.669724770642202	26.325
>50	0.327653997378768	10.65
>100	0.1310615989515072	5.949999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	127	3.175	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	111	2.775	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	98	2.45	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	97	2.4250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	95	2.375	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	78	1.95	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	46	1.15	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	40	1.0	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	35	0.8750000000000001	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	30	0.75	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	28	0.7000000000000001	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	25	0.625	RNA PCR Primer, Index 1 (100% over 33bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	24	0.6	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	20	0.5	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	18	0.44999999999999996	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	14	0.35000000000000003	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	13	0.325	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	13	0.325	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	11	0.27499999999999997	RNA PCR Primer, Index 1 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	10	0.25	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	10	0.25	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	9	0.22499999999999998	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	9	0.22499999999999998	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	8	0.2	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTC	8	0.2	RNA PCR Primer, Index 40 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 40 (100% over 50bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC	6	0.15	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	6	0.15	No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAATTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TATGCAAGGGCAAGCTCTCTTCATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	5	0.125	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	5	0.125	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	5	0.125	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AAGAAAGATTGCAAGAACTGCTAATTCATGCTTCCATGTTTAAAAACATG	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCAATTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.175	0.0	0.0	0.0
2	0.0	0.225	0.0	0.0	0.0
3	0.0	0.275	0.0	0.0	0.0
4	0.0	0.3	0.0	0.0	0.0
5	0.0	0.325	0.0	0.0	0.0
6	0.0	0.35	0.0	0.0	0.0
7	0.0	0.575	0.0	0.0	0.0
8	0.0	0.7	0.0	0.0	0.0
9	0.0	0.725	0.0	0.0	0.0
10	0.0	0.85	0.0	0.0	0.0
11	0.0	0.925	0.0	0.0	0.0
12	0.0	1.025	0.0	0.0	0.0
13	0.0	1.175	0.0	0.0	0.0
14	0.0	1.375	0.0	0.0	0.0
15	0.0	1.425	0.0	0.0	0.0
16	0.0	2.125	0.0	0.0	0.0
17	0.0	2.5	0.0	0.0	0.0
18	0.0	4.05	0.0	0.0	0.0
19	0.0	4.375	0.0	0.0	0.0
20	0.0	5.375	0.0	0.0	0.0
21	0.0	12.05	0.0	0.0	0.0
22	0.0	23.15	0.0	0.0	0.0
23	0.0	50.225	0.0	0.0	0.0
24	0.0	62.75	0.0	0.0	0.0
25	0.0	69.125	0.0	0.0	0.0
26	0.0	70.475	0.0	0.0	0.0
27	0.0	70.95	0.0	0.0	0.0
28	0.0	71.45	0.0	0.0	0.0
29	0.0	71.975	0.0	0.0	0.0
30	0.0	72.45	0.0	0.0	0.0
31	0.0	73.075	0.0	0.0	0.0
32	0.0	73.775	0.0	0.0	0.0
33	0.0	74.725	0.0	0.0	0.0
34	0.0	76.3	0.0	0.0	0.0
35	0.0	76.8	0.0	0.0	0.0
36	0.0	77.25	0.0	0.0	0.0
37	0.0	77.625	0.0	0.0	0.0
38	0.0	78.025	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.952051E-4	44.000004	2
GAGTGGA	20	6.952051E-4	44.000004	20
GAGCTTG	65	0.0	44.000004	13
GGATCCG	65	0.0	44.000004	3
CCGTCTG	65	0.0	44.000004	7
TCGGATC	65	0.0	44.000004	1
CGGATCC	65	0.0	44.000004	2
GTGAGTG	20	6.952051E-4	44.000004	18
CTTGGTG	20	6.952051E-4	44.000004	16
GATCCGT	65	0.0	44.000004	4
GGCTTGG	40	5.315087E-9	44.000004	19
GGTAGTA	20	6.952051E-4	44.000004	4
GCTTGGT	20	6.952051E-4	44.000004	15
GCTTGGC	40	5.315087E-9	44.000004	15
TTGGCTT	20	6.952051E-4	44.000004	17
TTGGTGG	20	6.952051E-4	44.000004	17
TCCGTCT	65	0.0	44.000004	6
AGGTAGT	20	6.952051E-4	44.000004	3
ATCCGTC	65	0.0	44.000004	5
TGGTGGA	20	6.952051E-4	44.000004	18
>>END_MODULE
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226857 spots for SRR5030858.sra
Written 226857 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
Read 226849 spots for SRR5030858.sra
Written 226849 spots for SRR5030858.sra
SRR ids: ['SRR5030858.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7uwfh3sj
SRR5030858.sra spots: 2722196
blocks: [[1, 226849], [226850, 453698], [453699, 680547], [680548, 907396], [907397, 1134245], [1134246, 1361094], [1361095, 1587943], [1587944, 1814792], [1814793, 2041641], [2041642, 2268490], [2268491, 2495339], [2495340, 2722196]]
SRR5030858 file size 470737
SRR5030858 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030858 SRR5030858_1.fastq
Input file:	SRR5030858_1.fastq
trimmed:	SRR5030858-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:29:52 2020 >> started

Wed Mar  4 06:29:54 2020 >> done (1.678s)
2722196 reads processed; of these:
    188 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
2722004 (99.99%) reads available; of these:
 199876 ( 7.34%) trimmed reads available after processing
2522128 (92.66%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     42	  0.00%
 19	     54	  0.00%
 20	    210	  0.01%
 21	    215	  0.01%
 22	    349	  0.01%
 23	    422	  0.02%
 24	    391	  0.01%
 25	    472	  0.02%
 26	    475	  0.02%
 27	    621	  0.02%
 28	    699	  0.03%
 29	   1120	  0.04%
 30	    859	  0.03%
 31	   1096	  0.04%
 32	   1500	  0.06%
 33	   2397	  0.09%
 34	   6687	  0.25%
 35	   3247	  0.12%
 36	   2410	  0.09%
 37	   2409	  0.09%
 38	   3935	  0.14%
 39	   3298	  0.12%
 40	   5310	  0.20%
 41	   4313	  0.16%
 42	   6953	  0.26%
 43	   5773	  0.21%
 44	  15647	  0.57%
 45	  12642	  0.46%
 46	  12218	  0.45%
 47	  15242	  0.56%
 48	  35583	  1.31%
 49	  53287	  1.96%
 50	2522128	 92.66%
2722004 reads passed initial QC


criterion=sequence-density
sequence-density=76.38
sequence-density-rank=1
fanout-score=21.58
fanout-score-rank=2
prefix-density=78.97
prefix-fanout=20.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC


criterion=fanout-score
sequence-density=2.25
sequence-density-rank=5
fanout-score=107.68
fanout-score-rank=1
prefix-density=77.86
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC -o SRR5030858 -
Input file:	STDIN
trimmed:	SRR5030858-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:30:07 2020 >> started

Wed Mar  4 06:30:11 2020 >> done (3.707s)
2651303 reads processed; of these:
 111894 ( 4.22%) short reads filtered out after trimming by size control
   5241 ( 0.20%) empty reads filtered out after trimming by size control
2534168 (95.58%) reads available; of these:
2192018 (86.50%) trimmed reads available after processing
 342150 (13.50%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12446	  0.49%
 19	  23607	  0.93%
 20	 163175	  6.44%
 21	 296679	 11.71%
 22	 718877	 28.37%
 23	 325919	 12.86%
 24	 152031	  6.00%
 25	  40526	  1.60%
 26	  19797	  0.78%
 27	  15231	  0.60%
 28	  17032	  0.67%
 29	  11893	  0.47%
 30	  16906	  0.67%
 31	  23564	  0.93%
 32	  23118	  0.91%
 33	  43869	  1.73%
 34	  17335	  0.68%
 35	  14328	  0.57%
 36	  14858	  0.59%
 37	  12190	  0.48%
 38	  13315	  0.53%
 39	  15127	  0.60%
 40	  26626	  1.05%
 41	  35146	  1.39%
 42	  42250	  1.67%
 43	  29361	  1.16%
 44	  22083	  0.87%
 45	  30854	  1.22%
 46	  26144	  1.03%
 47	  16317	  0.64%
 48	   4246	  0.17%
 49	  12638	  0.50%
 50	 296680	 11.71%


criterion=sequence-density
sequence-density=5.52
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=39
fanout-score=43.75
fanout-score-rank=1
prefix-density=1.04
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:30:31
                             Started mapping on |	Mar 04 06:30:32
                                    Finished on |	Mar 04 06:30:55
       Mapping speed, Million of reads per hour |	407.72

                          Number of input reads |	2604869
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1549754
                        Uniquely mapped reads % |	59.49%
                          Average mapped length |	27.88
                       Number of splices: Total |	1073
            Number of splices: Annotated (sjdb) |	177
                       Number of splices: GT/AG |	953
                       Number of splices: GC/AG |	16
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	103
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.57
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	739660
             % of reads mapped to multiple loci |	28.40%
        Number of reads mapped to too many loci |	44269
             % of reads mapped to too many loci |	1.70%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.95%
                     % of reads unmapped: other |	0.46%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	315455	315455	315455
N_multimapping	739660	739660	739660
N_noFeature	67511	89259	1060594
N_ambiguous	868870	454885	21249
UnstrandedReadsAssigned:613373 PositiveStrandReadsAssigned:1005610 NegativeStrandReadsAssigned:467911
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030858 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030858-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,604,869 reads, 703,985 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,072 rounds

 109283 SRR5030858.ke.tsv
  52636 SRR5030858.se.tsv
 161919 total
==> SRR5030858.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030858.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030858 completed mapping pipeline successfully
