Starting /dee2/code/volunteer_pipeline.sh SRR5031095
    current disk space = 160484474880
    free memory = 73950372 
SRR5031095 SRAfilesize
ce498e9631432d89abf8f31f22f1ad8d  SRR5031095.sra
SRR5031095.sra file validated
SRR5031095 is single end
SRR5031095 is conventional basespace
SRR5031095 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.944	31.0	31.0	34.0	30.0	34.0
2	32.388	33.0	31.0	34.0	31.0	34.0
3	32.37425	33.0	31.0	34.0	31.0	34.0
4	36.13875	37.0	35.0	37.0	35.0	37.0
5	36.11875	37.0	35.0	37.0	35.0	37.0
6	35.9485	37.0	35.0	37.0	35.0	37.0
7	35.765	37.0	35.0	37.0	35.0	37.0
8	35.56	37.0	35.0	37.0	33.0	37.0
9	36.81175	39.0	37.0	39.0	33.0	39.0
10	36.35575	39.0	35.0	39.0	32.0	39.0
11	37.2475	39.0	37.0	39.0	34.0	39.0
12	37.54525	39.0	37.0	39.0	35.0	39.0
13	37.24775	39.0	37.0	39.0	34.0	39.0
14	38.2075	40.0	37.0	41.0	33.0	41.0
15	36.825	39.0	36.0	41.0	30.0	41.0
16	36.543	38.0	36.0	40.0	31.0	41.0
17	36.6515	39.0	36.0	40.0	30.0	41.0
18	38.08625	40.0	37.0	41.0	34.0	41.0
19	38.5485	40.0	38.0	41.0	35.0	41.0
20	38.40325	40.0	38.0	41.0	34.0	41.0
21	38.57675	40.0	38.0	41.0	34.0	41.0
22	37.501	39.0	37.0	40.0	32.0	41.0
23	37.89875	39.0	37.0	40.0	34.0	41.0
24	37.19625	39.0	37.0	40.0	31.0	41.0
25	38.11075	40.0	37.0	41.0	33.0	41.0
26	38.1305	40.0	38.0	41.0	34.0	41.0
27	38.2955	40.0	38.0	41.0	34.0	41.0
28	38.55625	40.0	38.0	41.0	34.0	41.0
29	38.5085	40.0	38.0	41.0	34.0	41.0
30	38.83175	40.0	38.0	41.0	35.0	41.0
31	38.94	40.0	38.0	41.0	35.0	41.0
32	38.92225	40.0	38.0	41.0	35.0	41.0
33	38.969	40.0	39.0	41.0	35.0	41.0
34	38.41225	40.0	38.0	41.0	34.0	41.0
35	37.288	40.0	36.0	41.0	30.0	41.0
36	33.0255	35.0	30.0	40.0	23.0	41.0
37	34.67025	37.0	32.0	39.0	28.0	40.0
38	36.20075	38.0	35.0	39.0	30.0	40.0
39	36.6265	38.0	36.0	40.0	31.0	40.0
40	37.121	39.0	37.0	40.0	32.0	41.0
41	37.5295	40.0	37.0	40.0	33.0	41.0
42	37.45725	40.0	37.0	41.0	32.0	41.0
43	37.28925	40.0	37.0	41.0	31.0	41.0
44	36.4955	39.0	36.0	40.0	30.0	41.0
45	36.5015	39.0	36.0	40.0	31.0	41.0
46	36.68075	39.0	36.0	40.0	30.0	41.0
47	35.76775	38.0	35.0	40.0	28.0	41.0
48	36.8195	39.0	37.0	40.0	31.0	41.0
49	36.971	40.0	37.0	41.0	32.0	41.0
50	35.276	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	6.0
21	12.0
22	6.0
23	6.0
24	11.0
25	14.0
26	18.0
27	14.0
28	22.0
29	37.0
30	52.0
31	73.0
32	92.0
33	140.0
34	178.0
35	297.0
36	414.0
37	733.0
38	1172.0
39	700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.902951475737869	26.338169084542272	56.428214107053535	11.330665332666333
2	23.56178089044522	28.8144072036018	15.407703851925964	32.21610805402702
3	24.2	24.375	18.925	32.5
4	32.95	29.549999999999997	11.575000000000001	25.924999999999997
5	21.125	42.925000000000004	26.224999999999998	9.725
6	35.3	14.249999999999998	37.75	12.7
7	11.625	21.875	25.474999999999998	41.025
8	26.424999999999997	30.0	13.425	30.15
9	21.025	37.724999999999994	28.325	12.925
10	18.5	24.474999999999998	28.525	28.499999999999996
11	32.45	7.475	15.4	44.675
12	13.350000000000001	16.3	32.35	38.0
13	33.625	14.825	33.525	18.025
14	36.275	30.25	20.05	13.425
15	36.65	18.8	29.375	15.174999999999999
16	16.650000000000002	18.175	21.0	44.175
17	24.825	19.950000000000003	41.525	13.700000000000001
18	26.474999999999998	16.7	28.499999999999996	28.325
19	36.55	13.825000000000001	33.324999999999996	16.3
20	48.449999999999996	12.2	27.700000000000003	11.65
21	20.4	20.225	31.8	27.575
22	29.299999999999997	8.325000000000001	52.800000000000004	9.575
23	27.675	12.375	48.775	11.175
24	54.525	10.65	27.1	7.725
25	53.574999999999996	26.125	14.6	5.7
26	28.625	51.87500000000001	12.025	7.475
27	14.025000000000002	52.400000000000006	26.25	7.324999999999999
28	8.375	26.55	53.425	11.65
29	8.225	11.125	59.724999999999994	20.925
30	6.800000000000001	4.775	42.575	45.85
31	12.049999999999999	3.025	48.05	36.875
32	28.075	3.8	22.475	45.65
33	59.150000000000006	5.575	12.275	23.0
34	70.75	4.375	12.7	12.174999999999999
35	69.75	4.45	19.650000000000002	6.15
36	49.375	4.375	35.55	10.7
37	47.65	7.825	19.35	25.174999999999997
38	22.625	13.750000000000002	11.025	52.6
39	13.350000000000001	26.424999999999997	6.9750000000000005	53.25
40	12.875	53.125	7.000000000000001	27.0
41	26.474999999999998	52.87500000000001	5.6000000000000005	15.049999999999999
42	53.37499999999999	32.324999999999996	6.275	8.025
43	54.25	33.5	4.75	7.5
44	30.375000000000004	54.2	4.175	11.25
45	16.575	53.75	9.700000000000001	19.975
46	9.1	27.3	19.6	44.0
47	5.7	14.424999999999999	38.275	41.6
48	7.124999999999999	12.125	20.075000000000003	60.675000000000004
49	11.15	20.424999999999997	11.425	56.99999999999999
50	20.1	38.1	10.85	30.95
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.0
26	1.0
27	0.5
28	0.0
29	0.5
30	1.0
31	1.5
32	2.0
33	3.0
34	4.0
35	8.0
36	12.0
37	13.0
38	14.0
39	16.0
40	18.0
41	20.5
42	23.0
43	63.5
44	104.0
45	141.0
46	178.0
47	312.0
48	446.0
49	545.5
50	645.0
51	852.0
52	1059.0
53	760.5
54	462.0
55	473.5
56	485.0
57	375.0
58	265.0
59	179.0
60	93.0
61	63.0
62	33.0
63	56.0
64	79.0
65	52.5
66	26.0
67	21.5
68	17.0
69	11.5
70	6.0
71	14.0
72	22.0
73	11.5
74	1.0
75	0.5
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.675000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32379004771643	27.625
2	10.020449897750511	7.35
3	3.408316291751875	3.75
4	2.1813224267212	3.2
5	2.1813224267212	4.0
6	1.2951601908657124	2.85
7	0.7498295841854125	1.925
8	0.408997955010225	1.2
9	0.47716428084526247	1.575
>10	3.3401499659168374	23.65
>50	0.34083162917518744	9.5
>100	0.27266530334015	13.375
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	149	3.7249999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	135	3.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	134	3.35	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	117	2.9250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	100	2.5	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	98	2.45	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	57	1.425	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	53	1.325	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	47	1.175	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (96% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGC	18	0.44999999999999996	RNA PCR Primer, Index 19 (100% over 50bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	12	0.3	RNA PCR Primer, Index 1 (100% over 33bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC	11	0.27499999999999997	RNA PCR Primer, Index 19 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 25bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	9	0.22499999999999998	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	8	0.2	RNA PCR Primer, Index 15 (97% over 36bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGACCCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	6	0.15	RNA PCR Primer, Index 6 (100% over 34bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTCACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG	5	0.125	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGGCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
TGTAAACATCCTACCCTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGGGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	5	0.125	RNA PCR Primer, Index 1 (97% over 35bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.55	0.0	0.0	0.0
2	0.0	0.55	0.0	0.0	0.0
3	0.0	0.675	0.0	0.0	0.0
4	0.0	0.7	0.0	0.0	0.0
5	0.0	0.7	0.0	0.0	0.0
6	0.0	0.775	0.0	0.0	0.0
7	0.0	1.125	0.0	0.0	0.0
8	0.0	1.35	0.0	0.0	0.0
9	0.0	1.45	0.0	0.0	0.0
10	0.0	1.5	0.0	0.0	0.0
11	0.0	1.625	0.0	0.0	0.0
12	0.0	1.775	0.0	0.0	0.0
13	0.0	1.825	0.0	0.0	0.0
14	0.0	2.05	0.0	0.0	0.0
15	0.0	2.525	0.0	0.0	0.0
16	0.0	3.35	0.0	0.0	0.0
17	0.0	4.125	0.0	0.0	0.0
18	0.0	4.8	0.0	0.0	0.0
19	0.0	5.225	0.0	0.0	0.0
20	0.0	6.0	0.0	0.0	0.0
21	0.0	10.85	0.0	0.0	0.0
22	0.0	26.075	0.0	0.0	0.0
23	0.0	59.575	0.0	0.0	0.0
24	0.0	75.55	0.0	0.0	0.0
25	0.0	82.15	0.0	0.0	0.0
26	0.0	83.8	0.0	0.0	0.0
27	0.0	84.625	0.0	0.0	0.0
28	0.0	85.075	0.0	0.0	0.0
29	0.0	85.625	0.0	0.0	0.0
30	0.0	86.05	0.0	0.0	0.0
31	0.0	86.575	0.0	0.0	0.0
32	0.0	87.175	0.0	0.0	0.0
33	0.0	87.9	0.0	0.0	0.0
34	0.0	90.275	0.0	0.0	0.0
35	0.0	90.95	0.0	0.0	0.0
36	0.0	91.325	0.0	0.0	0.0
37	0.0	91.825	0.0	0.0	0.0
38	0.0	92.075	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGATT	20	6.952051E-4	44.000004	8
GTTTGGA	20	6.952051E-4	44.000004	20
AGTAGAT	20	6.952051E-4	44.000004	7
GATTGTA	20	6.952051E-4	44.000004	11
GTGAGTT	20	6.952051E-4	44.000004	18
ACATTCA	40	5.315087E-9	44.000004	2
GAGTTGG	20	6.952051E-4	44.000004	20
ATAGTTT	20	6.952051E-4	44.000004	17
TAGTAGA	20	6.952051E-4	44.000004	6
ATTGTAT	20	6.952051E-4	44.000004	12
CTGTTGG	20	6.952051E-4	44.000004	19
AGATTGT	20	6.952051E-4	44.000004	10
TGAGTTG	20	6.952051E-4	44.000004	19
TAGATTG	20	6.952051E-4	44.000004	9
GTCGGTG	40	5.315087E-9	44.000004	14
AACATTC	40	5.315087E-9	44.000004	1
TGTCGGT	40	5.315087E-9	44.000004	13
AACGCTG	30	1.9515464E-6	44.0	8
GAGGTAG	35	1.02281774E-7	44.0	2
GGTGAGT	30	1.9515464E-6	44.0	17
>>END_MODULE
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157404 spots for SRR5031095.sra
Written 157404 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
Read 157399 spots for SRR5031095.sra
Written 157399 spots for SRR5031095.sra
SRR ids: ['SRR5031095.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_62igmply
SRR5031095.sra spots: 1888793
blocks: [[1, 157399], [157400, 314798], [314799, 472197], [472198, 629596], [629597, 786995], [786996, 944394], [944395, 1101793], [1101794, 1259192], [1259193, 1416591], [1416592, 1573990], [1573991, 1731389], [1731390, 1888793]]
SRR5031095 file size 326301
SRR5031095 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031095 SRR5031095_1.fastq
Input file:	SRR5031095_1.fastq
trimmed:	SRR5031095-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:29 2020 >> started

Wed Mar  4 07:22:30 2020 >> done (1.064s)
1888793 reads processed; of these:
    115 ( 0.01%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
1888673 (99.99%) reads available; of these:
 147424 ( 7.81%) trimmed reads available after processing
1741249 (92.19%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     45	  0.00%
 19	     68	  0.00%
 20	    106	  0.01%
 21	    148	  0.01%
 22	    259	  0.01%
 23	    291	  0.02%
 24	    315	  0.02%
 25	    442	  0.02%
 26	    295	  0.02%
 27	    413	  0.02%
 28	    501	  0.03%
 29	    825	  0.04%
 30	    454	  0.02%
 31	    509	  0.03%
 32	    764	  0.04%
 33	   1632	  0.09%
 34	   2176	  0.12%
 35	   5822	  0.31%
 36	   3306	  0.18%
 37	   2982	  0.16%
 38	   2633	  0.14%
 39	   2809	  0.15%
 40	   3322	  0.18%
 41	   3599	  0.19%
 42	   4505	  0.24%
 43	   6905	  0.37%
 44	   7589	  0.40%
 45	   9811	  0.52%
 46	  12737	  0.67%
 47	  11398	  0.60%
 48	  30814	  1.63%
 49	  29949	  1.59%
 50	1741249	 92.19%
1888673 reads passed initial QC


criterion=sequence-density
sequence-density=92.28
sequence-density-rank=1
fanout-score=19.41
fanout-score-rank=2
prefix-density=93.07
prefix-fanout=19.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCC


criterion=fanout-score
sequence-density=4.09
sequence-density-rank=2
fanout-score=62.38
fanout-score-rank=1
prefix-density=93.15
prefix-fanout=2.7
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCC -o SRR5031095 -
Input file:	STDIN
trimmed:	SRR5031095-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:43 2020 >> started

Wed Mar  4 07:22:45 2020 >> done (2.108s)
1848057 reads processed; of these:
  86320 ( 4.67%) short reads filtered out after trimming by size control
  11727 ( 0.63%) empty reads filtered out after trimming by size control
1750010 (94.69%) reads available; of these:
1713606 (97.92%) trimmed reads available after processing
  36404 ( 2.08%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   9054	  0.52%
 19	  18981	  1.08%
 20	 110581	  6.32%
 21	 262793	 15.02%
 22	 650901	 37.19%
 23	 297881	 17.02%
 24	 132194	  7.55%
 25	  31910	  1.82%
 26	  13975	  0.80%
 27	  10372	  0.59%
 28	  10383	  0.59%
 29	   8221	  0.47%
 30	  10630	  0.61%
 31	  12764	  0.73%
 32	  14242	  0.81%
 33	  39724	  2.27%
 34	  12276	  0.70%
 35	   8997	  0.51%
 36	  11019	  0.63%
 37	   4633	  0.26%
 38	   4870	  0.28%
 39	   4264	  0.24%
 40	   6084	  0.35%
 41	   7293	  0.42%
 42	   7994	  0.46%
 43	   5301	  0.30%
 44	   3723	  0.21%
 45	   4660	  0.27%
 46	   3129	  0.18%
 47	   2496	  0.14%
 48	    441	  0.03%
 49	    560	  0.03%
 50	  27664	  1.58%


criterion=sequence-density
sequence-density=9.38
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.18
sequence-density-rank=7
fanout-score=18.90
fanout-score-rank=1
prefix-density=2.20
prefix-fanout=18.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGC
                                 Started job on |	Mar 04 07:23:03
                             Started mapping on |	Mar 04 07:23:03
                                    Finished on |	Mar 04 07:23:28
       Mapping speed, Million of reads per hour |	257.85

                          Number of input reads |	1790626
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	937707
                        Uniquely mapped reads % |	52.37%
                          Average mapped length |	23.03
                       Number of splices: Total |	3987
            Number of splices: Annotated (sjdb) |	867
                       Number of splices: GT/AG |	3934
                       Number of splices: GC/AG |	31
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	22
                      Mismatch rate per base, % |	0.38%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.81
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	710781
             % of reads mapped to multiple loci |	39.69%
        Number of reads mapped to too many loci |	19091
             % of reads mapped to too many loci |	1.07%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.49%
                     % of reads unmapped: other |	0.38%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	142138	142138	142138
N_multimapping	710781	710781	710781
N_noFeature	50664	57439	641420
N_ambiguous	531572	268353	4825
UnstrandedReadsAssigned:355471 PositiveStrandReadsAssigned:611915 NegativeStrandReadsAssigned:291462
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031095 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031095-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,790,626 reads, 457,634 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,053 rounds

 109283 SRR5031095.ke.tsv
  52636 SRR5031095.se.tsv
 161919 total
==> SRR5031095.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031095.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031095 completed mapping pipeline successfully
