Starting /dee2/code/volunteer_pipeline.sh SRR5030964
    current disk space = 160602243072
    free memory = 73943928 
SRR5030964 SRAfilesize
d09c03fb9ecb6cc4517a98efe1ba0860  SRR5030964.sra
SRR5030964.sra file validated
SRR5030964 is single end
SRR5030964 is conventional basespace
SRR5030964 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57675	34.0	31.0	34.0	31.0	34.0
2	32.81875	34.0	31.0	34.0	31.0	34.0
3	32.896	34.0	31.0	34.0	31.0	34.0
4	36.2795	37.0	37.0	37.0	35.0	37.0
5	36.34225	37.0	37.0	37.0	35.0	37.0
6	36.20675	37.0	37.0	37.0	35.0	37.0
7	36.18475	37.0	36.0	37.0	35.0	37.0
8	36.22825	37.0	36.0	37.0	35.0	37.0
9	38.00225	39.0	38.0	39.0	35.0	39.0
10	38.074	39.0	39.0	39.0	35.0	39.0
11	37.9855	39.0	38.0	39.0	35.0	39.0
12	38.023	39.0	38.0	39.0	35.0	39.0
13	37.8935	39.0	38.0	39.0	35.0	39.0
14	39.3275	41.0	39.0	41.0	36.0	41.0
15	39.4405	41.0	39.0	41.0	36.0	41.0
16	39.3195	41.0	39.0	41.0	36.0	41.0
17	39.46	41.0	39.0	41.0	36.0	41.0
18	39.35925	41.0	39.0	41.0	36.0	41.0
19	39.2395	41.0	39.0	41.0	36.0	41.0
20	39.2285	41.0	39.0	41.0	36.0	41.0
21	39.3145	41.0	39.0	41.0	36.0	41.0
22	39.254	41.0	39.0	41.0	36.0	41.0
23	39.06125	40.0	39.0	41.0	36.0	41.0
24	38.78125	40.0	38.0	41.0	35.0	41.0
25	39.15325	41.0	39.0	41.0	36.0	41.0
26	39.232	41.0	39.0	41.0	36.0	41.0
27	39.23675	41.0	39.0	41.0	36.0	41.0
28	39.0315	40.0	39.0	41.0	35.0	41.0
29	38.341	40.0	38.0	41.0	33.0	41.0
30	38.5025	40.0	38.0	41.0	34.0	41.0
31	38.81925	40.0	38.0	41.0	35.0	41.0
32	39.03475	40.0	38.0	41.0	35.0	41.0
33	38.10575	40.0	38.0	41.0	33.0	41.0
34	38.06975	40.0	38.0	41.0	33.0	41.0
35	37.89725	40.0	37.0	41.0	33.0	41.0
36	34.75625	38.0	33.0	40.0	23.0	41.0
37	35.87325	38.0	35.0	40.0	30.0	41.0
38	37.34175	39.0	37.0	40.0	32.0	41.0
39	37.4835	39.0	37.0	40.0	33.0	41.0
40	37.44675	40.0	37.0	41.0	32.0	41.0
41	37.456	40.0	37.0	41.0	33.0	41.0
42	37.65575	40.0	38.0	41.0	33.0	41.0
43	37.365	40.0	37.0	41.0	32.0	41.0
44	37.4145	40.0	37.0	41.0	33.0	41.0
45	37.02075	40.0	36.0	41.0	31.0	41.0
46	35.35975	38.0	34.0	40.0	27.0	41.0
47	35.856	39.0	35.0	40.0	29.0	41.0
48	36.04875	39.0	36.0	40.0	30.0	41.0
49	35.18575	39.0	35.0	40.0	28.0	41.0
50	30.09825	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	1.0
20	4.0
21	1.0
22	11.0
23	9.0
24	9.0
25	14.0
26	12.0
27	21.0
28	24.0
29	34.0
30	47.0
31	63.0
32	63.0
33	127.0
34	149.0
35	191.0
36	314.0
37	504.0
38	1023.0
39	1377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.45286321580395	24.306076519129782	50.337584396099025	13.903475868967242
2	20.875	28.199999999999996	19.375	31.55
3	26.25	17.424999999999997	23.150000000000002	33.175
4	32.2	28.349999999999998	14.249999999999998	25.2
5	20.974999999999998	40.75	24.7	13.575000000000001
6	28.299999999999997	17.549999999999997	40.8	13.350000000000001
7	19.425	21.575	22.1	36.9
8	22.975	30.325000000000003	15.475	31.225
9	25.374999999999996	32.275	28.425	13.925
10	25.374999999999996	17.150000000000002	31.974999999999998	25.5
11	29.575000000000003	16.125	15.875	38.425
12	14.875	20.724999999999998	33.225	31.175000000000004
13	28.175	18.2	29.599999999999998	24.025
14	25.4	33.425	22.375	18.8
15	36.40910227556889	23.40585146286572	24.88122030507627	15.303825956489122
16	17.25	24.099999999999998	21.825	36.825
17	24.7	23.225	36.55	15.525
18	24.575	17.474999999999998	34.075	23.875
19	35.125	15.174999999999999	30.5	19.2
20	42.85	16.45	25.324999999999996	15.375
21	24.025	20.0	25.874999999999996	30.099999999999998
22	27.875	14.05	46.6	11.475
23	27.3	13.450000000000001	43.65	15.6
24	49.35	16.900000000000002	24.025	9.725
25	47.525	24.65	15.675	12.15
26	25.75	45.800000000000004	16.125	12.325
27	17.05	45.550000000000004	25.825	11.575000000000001
28	13.525	24.6	46.675	15.2
29	11.275	14.424999999999999	55.625	18.675
30	11.075	9.425	34.325	45.175
31	16.45	7.2749999999999995	44.275	32.0
32	27.250000000000004	7.3999999999999995	21.7	43.65
33	54.55	8.200000000000001	14.124999999999998	23.125
34	58.099999999999994	9.525	16.575	15.8
35	61.075	8.425	19.225	11.275
36	41.275	9.975000000000001	34.0	14.75
37	44.85	11.05	19.5	24.6
38	22.900000000000002	16.825000000000003	13.05	47.225
39	16.7	25.7	9.275	48.325
40	17.299999999999997	47.349999999999994	11.95	23.400000000000002
41	26.375	47.449999999999996	9.875	16.3
42	48.85	29.175	10.15	11.825
43	48.325	32.1	9.6	9.975000000000001
44	27.450000000000003	49.275000000000006	8.175	15.1
45	17.45	49.975	13.875000000000002	18.7
46	13.025	26.325	18.975	41.675000000000004
47	10.325	16.5	37.875	35.3
48	10.674999999999999	15.675	19.45	54.2
49	16.075	20.5	14.025000000000002	49.4
50	20.4801200300075	36.90922730682671	14.303575893973495	28.307076769192296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	2.0
26	3.0
27	3.0
28	3.0
29	2.5
30	2.0
31	7.0
32	12.0
33	24.5
34	37.0
35	46.0
36	55.0
37	61.0
38	67.0
39	72.0
40	77.0
41	68.0
42	59.0
43	83.0
44	107.0
45	156.0
46	205.0
47	301.5
48	398.0
49	502.5
50	607.0
51	675.5
52	744.0
53	593.5
54	443.0
55	496.0
56	549.0
57	439.0
58	329.0
59	209.5
60	90.0
61	74.5
62	59.0
63	60.0
64	61.0
65	44.5
66	28.0
67	23.5
68	19.0
69	13.0
70	7.0
71	16.0
72	25.0
73	13.5
74	2.0
75	1.5
76	1.0
77	1.0
78	1.0
79	3.0
80	5.0
81	3.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.449999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94451145958986	30.65
2	10.494571773220748	8.7
3	4.764776839565742	5.925
4	2.2316043425814236	3.6999999999999997
5	1.9300361881785282	4.0
6	1.2062726176115801	3.0
7	1.0253317249698433	2.9749999999999996
8	0.8443908323281062	2.8000000000000003
9	0.30156815440289503	1.125
>10	2.714113389626055	18.275
>50	0.4221954161640531	12.55
>100	0.12062726176115801	6.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	139	3.4750000000000005	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	113	2.825	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	95	2.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	85	2.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	77	1.925	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	61	1.525	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	60	1.5	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	56	1.4000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	38	0.95	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTC	32	0.8	RNA PCR Primer, Index 41 (100% over 44bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	23	0.575	RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	23	0.575	RNA PCR Primer, Index 7 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	22	0.5499999999999999	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (96% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	18	0.44999999999999996	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	18	0.44999999999999996	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 41 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	10	0.25	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	10	0.25	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	10	0.25	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	10	0.25	RNA PCR Primer, Index 1 (100% over 24bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	8	0.2	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	8	0.2	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	8	0.2	RNA PCR Primer, Index 1 (100% over 32bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 24bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	7	0.17500000000000002	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	7	0.17500000000000002	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	6	0.15	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	6	0.15	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCT	5	0.125	RNA PCR Primer, Index 41 (100% over 43bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTAT	5	0.125	RNA PCR Primer, Index 41 (100% over 48bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	5	0.125	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACTGCTAATGAAAACACAG	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	5	0.125	No Hit
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.35	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.575	0.0	0.0	0.0
4	0.0	0.6	0.0	0.0	0.0
5	0.0	0.6	0.0	0.0	0.0
6	0.0	0.725	0.0	0.0	0.0
7	0.0	1.575	0.0	0.0	0.0
8	0.0	2.0	0.0	0.0	0.0
9	0.0	2.075	0.0	0.0	0.0
10	0.0	2.275	0.0	0.0	0.0
11	0.0	2.525	0.0	0.0	0.0
12	0.0	2.75	0.0	0.0	0.0
13	0.0	3.075	0.0	0.0	0.0
14	0.0	3.4	0.0	0.0	0.0
15	0.0	3.725	0.0	0.0	0.0
16	0.0	4.875	0.0	0.0	0.0
17	0.0	5.6	0.0	0.0	0.0
18	0.0	6.6	0.0	0.0	0.0
19	0.0	7.375	0.0	0.0	0.0
20	0.0	7.925	0.0	0.0	0.0
21	0.0	14.175	0.0	0.0	0.0
22	0.0	24.925	0.0	0.0	0.0
23	0.0	53.0	0.0	0.0	0.0
24	0.0	63.85	0.0	0.0	0.0
25	0.0	69.35	0.0	0.0	0.0
26	0.0	71.025	0.0	0.0	0.0
27	0.0	71.975	0.0	0.0	0.0
28	0.0	72.4	0.0	0.0	0.0
29	0.0	72.925	0.0	0.0	0.0
30	0.0	73.325	0.0	0.0	0.0
31	0.0	74.05	0.0	0.0	0.0
32	0.0	74.95	0.0	0.0	0.0
33	0.0	75.775	0.0	0.0	0.0
34	0.0	77.575	0.0	0.0	0.0
35	0.0	78.4	0.0	0.0	0.0
36	0.0	79.175	0.0	0.0	0.0
37	0.0	80.025	0.0	0.0	0.0
38	0.0	80.675	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.952051E-4	44.000004	2
AGCTTGG	45	2.728484E-10	44.000004	14
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
TCGGATC	40	5.315087E-9	44.000004	1
CGGATCC	40	5.315087E-9	44.000004	2
CTTGGTG	20	6.952051E-4	44.000004	16
CTTGGCT	20	6.952051E-4	44.000004	16
GATCCGT	40	5.315087E-9	44.000004	4
GGTAGTA	20	6.952051E-4	44.000004	4
GCTTGGT	20	6.952051E-4	44.000004	15
TTGGTGG	20	6.952051E-4	44.000004	17
TCCGTCT	45	2.728484E-10	44.000004	6
ATCCGTC	45	2.728484E-10	44.000004	5
TGAGCTT	45	2.728484E-10	44.000004	12
CGTCTGA	45	2.728484E-10	44.000004	8
GTAGTAG	20	6.952051E-4	44.000004	5
AACGCTG	30	1.9515464E-6	44.0	8
TGCACTC	25	3.6958358E-5	44.0	4
>>END_MODULE
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177685 spots for SRR5030964.sra
Written 177685 spots for SRR5030964.sra
Read 177687 spots for SRR5030964.sra
Written 177687 spots for SRR5030964.sra
SRR ids: ['SRR5030964.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_pz9pdjal
SRR5030964.sra spots: 2132222
blocks: [[1, 177685], [177686, 355370], [355371, 533055], [533056, 710740], [710741, 888425], [888426, 1066110], [1066111, 1243795], [1243796, 1421480], [1421481, 1599165], [1599166, 1776850], [1776851, 1954535], [1954536, 2132222]]
SRR5030964 file size 368482
SRR5030964 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030964 SRR5030964_1.fastq
Input file:	SRR5030964_1.fastq
trimmed:	SRR5030964-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:55:18 2020 >> started

Wed Mar  4 06:55:19 2020 >> done (1.324s)
2132222 reads processed; of these:
     85 ( 0.00%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
2132134 (100.00%) reads available; of these:
 130588 ( 6.12%) trimmed reads available after processing
2001546 (93.88%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     32	  0.00%
 19	     28	  0.00%
 20	     87	  0.00%
 21	    101	  0.00%
 22	    185	  0.01%
 23	    222	  0.01%
 24	    243	  0.01%
 25	    334	  0.02%
 26	    244	  0.01%
 27	    378	  0.02%
 28	    475	  0.02%
 29	    695	  0.03%
 30	    509	  0.02%
 31	    656	  0.03%
 32	    586	  0.03%
 33	    827	  0.04%
 34	   1791	  0.08%
 35	   1512	  0.07%
 36	   1201	  0.06%
 37	   1342	  0.06%
 38	   1558	  0.07%
 39	   1866	  0.09%
 40	   2172	  0.10%
 41	   2294	  0.11%
 42	   3223	  0.15%
 43	   4175	  0.20%
 44	   7192	  0.34%
 45	   9410	  0.44%
 46	   8996	  0.42%
 47	  12883	  0.60%
 48	  24758	  1.16%
 49	  40613	  1.90%
 50	2001546	 93.88%
2132134 reads passed initial QC


criterion=sequence-density
sequence-density=80.03
sequence-density-rank=1
fanout-score=19.86
fanout-score-rank=1
prefix-density=82.02
prefix-fanout=19.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC


criterion=fanout-score
sequence-density=80.03
sequence-density-rank=1
fanout-score=19.86
fanout-score-rank=1
prefix-density=82.02
prefix-fanout=19.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC -o SRR5030964 -
Input file:	STDIN
trimmed:	SRR5030964-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:55:32 2020 >> started

Wed Mar  4 06:55:35 2020 >> done (3.251s)
2079489 reads processed; of these:
 132460 ( 6.37%) short reads filtered out after trimming by size control
  10298 ( 0.50%) empty reads filtered out after trimming by size control
1936731 (93.13%) reads available; of these:
1709540 (88.27%) trimmed reads available after processing
 227191 (11.73%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15125	  0.78%
 19	  17910	  0.92%
 20	 125190	  6.46%
 21	 227506	 11.75%
 22	 580209	 29.96%
 23	 233155	 12.04%
 24	 116652	  6.02%
 25	  31839	  1.64%
 26	  15390	  0.79%
 27	  11810	  0.61%
 28	  13180	  0.68%
 29	   9924	  0.51%
 30	  14649	  0.76%
 31	  20690	  1.07%
 32	  20965	  1.08%
 33	  38383	  1.98%
 34	  21445	  1.11%
 35	  18078	  0.93%
 36	  18648	  0.96%
 37	  10368	  0.54%
 38	  11152	  0.58%
 39	  11399	  0.59%
 40	  17477	  0.90%
 41	  21884	  1.13%
 42	  25135	  1.30%
 43	  17734	  0.92%
 44	  13962	  0.72%
 45	  19119	  0.99%
 46	  13778	  0.71%
 47	  10375	  0.54%
 48	   2387	  0.12%
 49	   7965	  0.41%
 50	 203248	 10.49%


criterion=sequence-density
sequence-density=6.52
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.21
sequence-density-rank=6
fanout-score=19.81
fanout-score-rank=1
prefix-density=2.29
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC
                                 Started job on |	Mar 04 06:55:57
                             Started mapping on |	Mar 04 06:55:57
                                    Finished on |	Mar 04 06:56:21
       Mapping speed, Million of reads per hour |	298.41

                          Number of input reads |	1989376
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1210530
                        Uniquely mapped reads % |	60.85%
                          Average mapped length |	27.24
                       Number of splices: Total |	948
            Number of splices: Annotated (sjdb) |	163
                       Number of splices: GT/AG |	885
                       Number of splices: GC/AG |	9
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	54
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	563618
             % of reads mapped to multiple loci |	28.33%
        Number of reads mapped to too many loci |	44362
             % of reads mapped to too many loci |	2.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.67%
                     % of reads unmapped: other |	0.92%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	215228	215228	215228
N_multimapping	563618	563618	563618
N_noFeature	45924	60616	811529
N_ambiguous	680975	330335	10320
UnstrandedReadsAssigned:483631 PositiveStrandReadsAssigned:819579 NegativeStrandReadsAssigned:388681
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030964 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030964-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,989,376 reads, 551,839 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,121 rounds

 109283 SRR5030964.ke.tsv
  52636 SRR5030964.se.tsv
 161919 total
==> SRR5030964.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030964.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030964 completed mapping pipeline successfully
