Starting /dee2/code/volunteer_pipeline.sh SRR5031070
    current disk space = 160508444672
    free memory = 73939660 
SRR5031070 SRAfilesize
a284c3484c097cebb343f89714ab6bc1  SRR5031070.sra
SRR5031070.sra file validated
SRR5031070 is single end
SRR5031070 is conventional basespace
SRR5031070 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031070_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31325	33.0	31.0	34.0	31.0	34.0
2	32.58625	34.0	31.0	34.0	31.0	34.0
3	32.64425	34.0	31.0	34.0	31.0	34.0
4	36.19425	37.0	35.0	37.0	35.0	37.0
5	35.78	37.0	35.0	37.0	35.0	37.0
6	35.81225	37.0	35.0	37.0	35.0	37.0
7	35.92475	37.0	35.0	37.0	35.0	37.0
8	35.803	37.0	35.0	37.0	35.0	37.0
9	37.5645	39.0	37.0	39.0	35.0	39.0
10	37.80775	39.0	37.0	39.0	35.0	39.0
11	37.64275	39.0	37.0	39.0	35.0	39.0
12	37.593	39.0	37.0	39.0	35.0	39.0
13	37.65975	39.0	37.0	39.0	35.0	39.0
14	39.0665	41.0	38.0	41.0	36.0	41.0
15	38.98875	40.0	38.0	41.0	35.0	41.0
16	39.07025	40.0	38.0	41.0	36.0	41.0
17	39.06225	40.0	38.0	41.0	36.0	41.0
18	39.02	41.0	38.0	41.0	35.0	41.0
19	38.6815	40.0	38.0	41.0	34.0	41.0
20	38.823	40.0	38.0	41.0	34.0	41.0
21	38.74425	40.0	38.0	41.0	34.0	41.0
22	38.88725	40.0	38.0	41.0	35.0	41.0
23	38.69825	40.0	38.0	41.0	34.0	41.0
24	38.68075	40.0	38.0	41.0	34.0	41.0
25	38.9135	40.0	39.0	41.0	35.0	41.0
26	38.64575	40.0	38.0	41.0	34.0	41.0
27	38.6425	40.0	38.0	41.0	34.0	41.0
28	38.567	40.0	38.0	41.0	34.0	41.0
29	38.18825	40.0	38.0	41.0	33.0	41.0
30	38.01425	40.0	38.0	41.0	33.0	41.0
31	38.28375	40.0	38.0	41.0	33.0	41.0
32	38.15475	40.0	38.0	41.0	33.0	41.0
33	38.1985	40.0	38.0	41.0	33.0	41.0
34	38.4005	40.0	38.0	41.0	34.0	41.0
35	38.1825	40.0	38.0	41.0	34.0	41.0
36	37.8645	40.0	37.0	41.0	33.0	41.0
37	37.42625	40.0	37.0	41.0	31.0	41.0
38	36.7245	39.0	36.0	41.0	30.0	41.0
39	37.2285	39.0	37.0	41.0	31.0	41.0
40	37.39825	40.0	37.0	41.0	31.0	41.0
41	37.43525	40.0	37.0	41.0	31.0	41.0
42	37.7185	40.0	37.0	41.0	33.0	41.0
43	38.03525	40.0	38.0	41.0	33.0	41.0
44	37.75675	40.0	37.0	41.0	33.0	41.0
45	37.74025	40.0	37.0	41.0	33.0	41.0
46	37.643	40.0	37.0	41.0	33.0	41.0
47	36.99	40.0	36.0	41.0	31.0	41.0
48	36.66075	39.0	36.0	41.0	30.0	41.0
49	36.82275	40.0	36.0	41.0	31.0	41.0
50	36.242	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	2.0
16	0.0
17	1.0
18	3.0
19	3.0
20	4.0
21	2.0
22	3.0
23	9.0
24	10.0
25	9.0
26	8.0
27	14.0
28	22.0
29	32.0
30	64.0
31	80.0
32	88.0
33	101.0
34	167.0
35	189.0
36	257.0
37	470.0
38	726.0
39	1733.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.725	24.075	46.7	18.5
2	22.8342513770656	26.89033550325488	18.803204807210815	31.4722083124687
3	23.7	19.025	23.1	34.175
4	33.625	26.075	16.175	24.125
5	23.275000000000002	36.575	26.55	13.600000000000001
6	32.95	14.424999999999999	35.375	17.25
7	17.474999999999998	21.825	22.7	38.0
8	25.275	27.525	16.950000000000003	30.25
9	23.724999999999998	30.95	27.175	18.15
10	21.725	21.75	27.700000000000003	28.825
11	33.4	11.1	16.925	38.574999999999996
12	18.275	18.224999999999998	28.675	34.825
13	29.525000000000002	16.7	33.25	20.525
14	29.599999999999998	30.925000000000004	20.150000000000002	19.325
15	35.325	22.45	27.224999999999998	15.0
16	18.85	19.075	24.55	37.525
17	28.799999999999997	20.5	35.8	14.899999999999999
18	24.0	16.025	31.35	28.625
19	36.4	15.575	30.075000000000003	17.95
20	44.6	14.75	26.650000000000002	14.000000000000002
21	23.275000000000002	20.75	27.250000000000004	28.725
22	28.849999999999998	15.1	43.375	12.675
23	28.000000000000004	13.475000000000001	42.0	16.525000000000002
24	49.925000000000004	15.55	25.575	8.95
25	48.4	24.5	15.024999999999999	12.075
26	28.725	44.074999999999996	14.725	12.475
27	15.925	44.15	23.724999999999998	16.2
28	12.65	23.375	47.725	16.25
29	14.625	12.25	54.25	18.875
30	12.325	7.049999999999999	34.699999999999996	45.925
31	16.0	5.2749999999999995	44.7	34.025
32	29.95	7.475	20.8	41.775
33	50.975	10.424999999999999	14.649999999999999	23.95
34	57.925000000000004	8.15	18.375	15.55
35	61.775000000000006	8.95	18.975	10.299999999999999
36	40.175	9.675	36.0	14.149999999999999
37	41.9	12.75	21.9	23.45
38	24.55	17.1	12.25	46.1
39	17.724999999999998	23.400000000000002	11.600000000000001	47.275
40	16.2	46.575	12.925	24.3
41	25.05	46.675	11.05	17.224999999999998
42	45.7	28.675	14.299999999999999	11.325000000000001
43	50.275000000000006	26.825	10.35	12.55
44	30.525000000000002	44.425	10.05	15.0
45	19.75	46.825	13.575000000000001	19.85
46	15.6	25.275	18.5	40.625
47	10.45	15.5	38.75	35.3
48	13.15	13.0	21.224999999999998	52.625
49	13.450000000000001	20.7	16.3	49.55
50	18.675	36.6	13.700000000000001	31.025000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	1.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.5
28	3.0
29	2.5
30	2.0
31	10.0
32	18.0
33	17.5
34	17.0
35	21.0
36	25.0
37	33.0
38	41.0
39	42.5
40	44.0
41	46.5
42	49.0
43	74.0
44	99.0
45	135.5
46	172.0
47	273.0
48	374.0
49	474.5
50	575.0
51	693.5
52	812.0
53	663.0
54	514.0
55	513.5
56	513.0
57	401.0
58	289.0
59	182.5
60	76.0
61	62.5
62	49.0
63	87.0
64	125.0
65	77.5
66	30.0
67	21.5
68	13.0
69	21.0
70	29.0
71	71.0
72	113.0
73	61.5
74	10.0
75	5.0
76	0.0
77	1.5
78	3.0
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.68602425015955	29.65
2	9.125717932354819	7.1499999999999995
3	4.722399489470326	5.55
4	2.361199744735163	3.6999999999999997
5	1.467772814294831	2.875
6	1.4039566049776642	3.3000000000000003
7	0.6381620931716656	1.7500000000000002
8	0.8934269304403318	2.8000000000000003
9	0.3190810465858328	1.125
>10	2.871729419272495	23.5
>50	0.3828972559029994	12.85
>100	0.12763241863433314	5.75
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	98	2.45	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	95	2.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	95	2.375	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	92	2.3	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	83	2.075	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	51	1.275	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	47	1.175	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	45	1.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	42	1.05	RNA PCR Primer, Index 7 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	38	0.95	RNA PCR Primer, Index 1 (100% over 33bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	38	0.95	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	30	0.75	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC	23	0.575	RNA PCR Primer, Index 42 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	19	0.475	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	18	0.44999999999999996	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC	18	0.44999999999999996	RNA PCR Primer, Index 42 (100% over 44bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	18	0.44999999999999996	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	13	0.325	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	11	0.27499999999999997	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	9	0.22499999999999998	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC	8	0.2	No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	8	0.2	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	8	0.2	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	8	0.2	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	8	0.2	No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT	6	0.15	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	6	0.15	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	6	0.15	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG	6	0.15	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	6	0.15	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	6	0.15	No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	6	0.15	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA	6	0.15	RNA PCR Primer, Index 3 (97% over 36bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG	5	0.125	RNA PCR Primer, Index 42 (100% over 39bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GACCCTGTTGATCTCAAATACTAATTGCCAGTTTTGTCTGATGTGGAATT	5	0.125	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	5	0.125	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	5	0.125	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCTGGAATT	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.625	0.0	0.0	0.0
2	0.0	0.625	0.0	0.0	0.0
3	0.0	0.7	0.0	0.0	0.0
4	0.0	0.7	0.0	0.0	0.0
5	0.0	0.825	0.0	0.0	0.0
6	0.0	0.875	0.0	0.0	0.0
7	0.0	1.5	0.0	0.0	0.0
8	0.0	1.775	0.0	0.0	0.0
9	0.0	1.9	0.0	0.0	0.0
10	0.0	2.0	0.0	0.0	0.0
11	0.0	2.15	0.0	0.0	0.0
12	0.0	2.45	0.0	0.0	0.0
13	0.0	2.7	0.0	0.0	0.0
14	0.0	2.9	0.0	0.0	0.0
15	0.0	3.7	0.0	0.0	0.0
16	0.0	5.175	0.0	0.0	0.0
17	0.0	5.6	0.0	0.0	0.0
18	0.0	7.25	0.0	0.0	0.0
19	0.0	7.7	0.0	0.0	0.0
20	0.0	8.775	0.0	0.0	0.0
21	0.0	13.85	0.0	0.0	0.0
22	0.0	24.275	0.0	0.0	0.0
23	0.0	51.125	0.0	0.0	0.0
24	0.0	63.25	0.0	0.0	0.0
25	0.0	68.3	0.0	0.0	0.0
26	0.0	69.475	0.0	0.0	0.0
27	0.0	70.3	0.0	0.0	0.0
28	0.0	70.975	0.0	0.0	0.0
29	0.0	71.8	0.0	0.0	0.0
30	0.0	72.275	0.0	0.0	0.0
31	0.0	73.5	0.0	0.0	0.0
32	0.0	74.4	0.0	0.0	0.0
33	0.0	75.6	0.0	0.0	0.0
34	0.0	79.425	0.0	0.0	0.0
35	0.0	80.575	0.0	0.0	0.0
36	0.0	81.7	0.0	0.0	0.0
37	0.0	82.975	0.0	0.0	0.0
38	0.0	83.75	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAGTA	25	3.4343255E-5	44.531647	2
TTCAAGT	25	3.4343255E-5	44.531647	1
TCGGATC	30	1.7904422E-6	44.531647	1
CGGATCC	30	1.7904422E-6	44.531647	2
ACATTCA	45	2.419256E-10	44.531647	2
AACATTC	45	2.419256E-10	44.531647	1
AAGTAAT	25	3.7082697E-5	43.975002	4
AATCCAG	25	3.7082697E-5	43.975002	8
AGCTTGG	30	1.9592044E-6	43.975002	14
ATCCAGG	25	3.7082697E-5	43.975002	9
GAGTGGA	25	3.7082697E-5	43.975002	20
AGGATAG	25	3.7082697E-5	43.975002	13
ATAGGCT	30	1.9592044E-6	43.975002	16
AGTGGAA	25	3.7082697E-5	43.975002	21
GAGCTTG	30	1.9592044E-6	43.975002	13
GGATCCG	30	1.9592044E-6	43.975002	3
CCGTCTG	30	1.9592044E-6	43.975002	7
TCCAGGA	25	3.7082697E-5	43.975002	10
GTGAGTG	25	3.7082697E-5	43.975002	18
GGATAGG	25	3.7082697E-5	43.975002	14
>>END_MODULE
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259607 spots for SRR5031070.sra
Written 259607 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
Read 259599 spots for SRR5031070.sra
Written 259599 spots for SRR5031070.sra
SRR ids: ['SRR5031070.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ymmu06p5
SRR5031070.sra spots: 3115196
blocks: [[1, 259599], [259600, 519198], [519199, 778797], [778798, 1038396], [1038397, 1297995], [1297996, 1557594], [1557595, 1817193], [1817194, 2076792], [2076793, 2336391], [2336392, 2595990], [2595991, 2855589], [2855590, 3115196]]
SRR5031070 file size 538870
SRR5031070 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031070 SRR5031070_1.fastq
Input file:	SRR5031070_1.fastq
trimmed:	SRR5031070-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:49 2020 >> started

Wed Mar  4 07:17:51 2020 >> done (1.859s)
3115196 reads processed; of these:
    247 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
3114945 (99.99%) reads available; of these:
 155213 ( 4.98%) trimmed reads available after processing
2959732 (95.02%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     64	  0.00%
 19	    122	  0.00%
 20	    225	  0.01%
 21	    216	  0.01%
 22	    414	  0.01%
 23	    463	  0.01%
 24	    490	  0.02%
 25	    707	  0.02%
 26	    535	  0.02%
 27	    688	  0.02%
 28	    783	  0.03%
 29	   1277	  0.04%
 30	    817	  0.03%
 31	    908	  0.03%
 32	    967	  0.03%
 33	   1354	  0.04%
 34	   1501	  0.05%
 35	   3020	  0.10%
 36	   2562	  0.08%
 37	   3095	  0.10%
 38	   2962	  0.10%
 39	   3170	  0.10%
 40	   3572	  0.11%
 41	   3460	  0.11%
 42	   4463	  0.14%
 43	   6349	  0.20%
 44	   8487	  0.27%
 45	  10661	  0.34%
 46	  16073	  0.52%
 47	  19980	  0.64%
 48	  27062	  0.87%
 49	  28766	  0.92%
 50	2959732	 95.02%
3114945 reads passed initial QC


criterion=sequence-density
sequence-density=83.20
sequence-density-rank=1
fanout-score=21.40
fanout-score-rank=1
prefix-density=85.48
prefix-fanout=20.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC


criterion=fanout-score
sequence-density=83.20
sequence-density-rank=1
fanout-score=21.40
fanout-score-rank=1
prefix-density=85.48
prefix-fanout=20.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC -o SRR5031070 -
Input file:	STDIN
trimmed:	SRR5031070-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:18:07 2020 >> started

Wed Mar  4 07:18:11 2020 >> done (4.785s)
3040780 reads processed; of these:
 201094 ( 6.61%) short reads filtered out after trimming by size control
  18471 ( 0.61%) empty reads filtered out after trimming by size control
2821215 (92.78%) reads available; of these:
2638297 (93.52%) trimmed reads available after processing
 182918 ( 6.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16624	  0.59%
 19	  33150	  1.18%
 20	 148679	  5.27%
 21	 302547	 10.72%
 22	 827245	 29.32%
 23	 383452	 13.59%
 24	 173126	  6.14%
 25	  49579	  1.76%
 26	  26054	  0.92%
 27	  21365	  0.76%
 28	  25197	  0.89%
 29	  20228	  0.72%
 30	  30653	  1.09%
 31	  34541	  1.22%
 32	  43278	  1.53%
 33	 106913	  3.79%
 34	  40442	  1.43%
 35	  31846	  1.13%
 36	  35618	  1.26%
 37	  19644	  0.70%
 38	  20507	  0.73%
 39	  19690	  0.70%
 40	  31759	  1.13%
 41	  39469	  1.40%
 42	  44570	  1.58%
 43	  30945	  1.10%
 44	  22291	  0.79%
 45	  34045	  1.21%
 46	  26553	  0.94%
 47	  17872	  0.63%
 48	   1751	  0.06%
 49	   2161	  0.08%
 50	 159421	  5.65%


criterion=sequence-density
sequence-density=6.76
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.60
sequence-density-rank=23
fanout-score=12.63
fanout-score-rank=1
prefix-density=7.58
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 07:18:34
                             Started mapping on |	Mar 04 07:18:34
                                    Finished on |	Mar 04 07:18:59
       Mapping speed, Million of reads per hour |	416.93

                          Number of input reads |	2895380
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1509795
                        Uniquely mapped reads % |	52.14%
                          Average mapped length |	25.48
                       Number of splices: Total |	2076
            Number of splices: Annotated (sjdb) |	377
                       Number of splices: GT/AG |	1969
                       Number of splices: GC/AG |	28
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	78
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.72
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.17
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	935450
             % of reads mapped to multiple loci |	32.31%
        Number of reads mapped to too many loci |	71261
             % of reads mapped to too many loci |	2.46%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.39%
                     % of reads unmapped: other |	0.70%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	450135	450135	450135
N_multimapping	935450	935450	935450
N_noFeature	74095	97773	1049533
N_ambiguous	832363	444335	16546
UnstrandedReadsAssigned:603337 PositiveStrandReadsAssigned:967687 NegativeStrandReadsAssigned:443716
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031070 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031070-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,895,380 reads, 697,877 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,146 rounds

 109283 SRR5031070.ke.tsv
  52636 SRR5031070.se.tsv
 161919 total
==> SRR5031070.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031070.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031070 completed mapping pipeline successfully
