Starting /dee2/code/volunteer_pipeline.sh SRR5030936
    current disk space = 160621182976
    free memory = 73943844 
SRR5030936 SRAfilesize
e21627c363fd99685bb7fd3d4d0dafbb  SRR5030936.sra
SRR5030936.sra file validated
SRR5030936 is single end
SRR5030936 is conventional basespace
SRR5030936 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39875	33.0	31.0	34.0	31.0	34.0
2	32.6335	34.0	31.0	34.0	31.0	34.0
3	32.7655	34.0	31.0	34.0	31.0	34.0
4	36.17875	37.0	37.0	37.0	35.0	37.0
5	36.214	37.0	37.0	37.0	35.0	37.0
6	36.0705	37.0	35.0	37.0	35.0	37.0
7	36.05625	37.0	35.0	37.0	35.0	37.0
8	36.1075	37.0	35.0	37.0	35.0	37.0
9	37.8425	39.0	38.0	39.0	35.0	39.0
10	37.91125	39.0	38.0	39.0	35.0	39.0
11	37.756	39.0	38.0	39.0	35.0	39.0
12	37.82	39.0	38.0	39.0	35.0	39.0
13	37.71725	39.0	38.0	39.0	35.0	39.0
14	39.196	41.0	39.0	41.0	36.0	41.0
15	39.1745	41.0	39.0	41.0	36.0	41.0
16	39.18025	41.0	39.0	41.0	36.0	41.0
17	39.3425	41.0	39.0	41.0	36.0	41.0
18	39.2315	41.0	39.0	41.0	36.0	41.0
19	38.92075	40.0	38.0	41.0	35.0	41.0
20	38.9795	40.0	38.0	41.0	35.0	41.0
21	39.09125	40.0	39.0	41.0	36.0	41.0
22	38.981	40.0	39.0	41.0	35.0	41.0
23	38.87475	40.0	39.0	41.0	35.0	41.0
24	38.7015	40.0	38.0	41.0	34.0	41.0
25	39.024	40.0	39.0	41.0	35.0	41.0
26	39.0415	40.0	39.0	41.0	35.0	41.0
27	38.92975	40.0	39.0	41.0	35.0	41.0
28	38.70925	40.0	38.0	41.0	35.0	41.0
29	37.99325	40.0	38.0	41.0	32.0	41.0
30	38.15875	40.0	38.0	41.0	33.0	41.0
31	38.3865	40.0	38.0	41.0	34.0	41.0
32	38.69	40.0	38.0	41.0	35.0	41.0
33	37.76375	40.0	38.0	41.0	32.0	41.0
34	37.6425	40.0	37.0	41.0	32.0	41.0
35	37.4475	39.0	37.0	40.0	33.0	41.0
36	33.74025	38.0	30.0	40.0	21.0	41.0
37	35.436	38.0	34.0	40.0	29.0	41.0
38	36.807	39.0	36.0	40.0	31.0	41.0
39	36.82525	39.0	36.0	40.0	31.0	41.0
40	36.9325	39.0	36.0	40.0	31.0	41.0
41	36.9165	39.0	36.0	40.0	31.0	41.0
42	37.16025	40.0	37.0	41.0	32.0	41.0
43	36.85175	39.0	36.0	40.0	31.0	41.0
44	36.7255	39.0	36.0	41.0	30.0	41.0
45	36.291	39.0	36.0	40.0	30.0	41.0
46	34.49875	38.0	33.0	40.0	24.0	41.0
47	35.1255	38.0	34.0	40.0	27.0	41.0
48	35.521	39.0	35.0	40.0	28.0	41.0
49	34.4875	38.0	34.0	40.0	26.0	41.0
50	29.3295	33.0	26.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	0.0
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	5.0
21	8.0
22	9.0
23	13.0
24	15.0
25	15.0
26	20.0
27	37.0
28	30.0
29	48.0
30	37.0
31	80.0
32	94.0
33	108.0
34	160.0
35	219.0
36	326.0
37	565.0
38	1105.0
39	1102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.084063047285463	23.29246935201401	49.23692769577183	15.386539904928698
2	22.525000000000002	25.275	20.599999999999998	31.6
3	26.1	17.625	21.95	34.325
4	33.35	26.875	14.000000000000002	25.775
5	21.725	38.574999999999996	23.375	16.325
6	29.225	16.875	39.15	14.75
7	18.95	19.975	24.099999999999998	36.975
8	23.549999999999997	29.875	15.675	30.9
9	26.1	32.05	27.450000000000003	14.399999999999999
10	25.124999999999996	18.65	32.4	23.825
11	28.499999999999996	16.400000000000002	16.275000000000002	38.824999999999996
12	15.725	20.225	33.725	30.325000000000003
13	28.975	18.95	29.575000000000003	22.5
14	26.150000000000002	32.85	20.825	20.175
15	36.159039759939986	23.15578894723681	25.256314078519633	15.428857214303576
16	16.6	23.025000000000002	22.625	37.75
17	23.625	21.575	37.8	17.0
18	22.925	17.9	36.199999999999996	22.975
19	36.825	16.400000000000002	28.725	18.05
20	43.4	16.725	25.374999999999996	14.499999999999998
21	24.025	19.400000000000002	26.424999999999997	30.15
22	26.974999999999998	15.625	46.35	11.05
23	26.200000000000003	14.625	42.275	16.900000000000002
24	48.075	17.325	24.975	9.625
25	47.125	24.25	15.1	13.525
26	27.450000000000003	43.45	16.525000000000002	12.575
27	18.75	44.224999999999994	23.724999999999998	13.3
28	14.499999999999998	24.2	44.975	16.325
29	11.175	15.45	54.800000000000004	18.575
30	11.85	8.225	33.95	45.975
31	15.9	7.3999999999999995	45.550000000000004	31.15
32	27.325	8.4	21.2	43.075
33	52.300000000000004	9.475	14.399999999999999	23.825
34	57.199999999999996	10.4	16.675	15.725
35	61.199999999999996	9.55	18.2	11.05
36	41.65	10.575	33.1	14.674999999999999
37	44.525	11.15	19.275000000000002	25.05
38	23.549999999999997	17.875	13.625000000000002	44.95
39	17.125	24.925	10.35	47.599999999999994
40	17.9	45.35	12.675	24.075
41	25.8	45.525	11.5	17.175
42	46.925	28.249999999999996	11.924999999999999	12.9
43	47.8	30.775000000000002	9.9	11.525
44	29.7	47.025	8.6	14.674999999999999
45	18.65	48.0	14.374999999999998	18.975
46	13.100000000000001	27.125	18.95	40.825
47	10.4	16.875	38.5	34.225
48	11.975	14.274999999999999	19.75	54.0
49	16.325	19.6	15.075	49.0
50	20.0	34.449999999999996	15.225	30.325000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.5
24	1.0
25	2.0
26	3.0
27	4.0
28	5.0
29	5.0
30	5.0
31	14.0
32	23.0
33	22.0
34	21.0
35	46.0
36	71.0
37	65.0
38	59.0
39	58.5
40	58.0
41	60.0
42	62.0
43	95.5
44	129.0
45	156.0
46	183.0
47	288.0
48	393.0
49	472.5
50	552.0
51	639.0
52	726.0
53	571.5
54	417.0
55	476.0
56	535.0
57	450.0
58	365.0
59	241.5
60	118.0
61	87.0
62	56.0
63	81.5
64	107.0
65	69.5
66	32.0
67	22.5
68	13.0
69	13.0
70	13.0
71	24.0
72	35.0
73	21.5
74	8.0
75	4.0
76	0.0
77	1.0
78	2.0
79	2.5
80	3.0
81	3.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11961722488039	31.4
2	10.107655502392344	8.450000000000001
3	4.007177033492823	5.025
4	2.0334928229665072	3.4000000000000004
5	1.1961722488038278	2.5
6	1.3157894736842104	3.3000000000000003
7	1.4952153110047848	4.375
8	0.5980861244019139	2.0
9	0.5382775119617225	2.025
>10	3.1698564593301435	23.3
>50	0.3588516746411483	11.1
>100	0.05980861244019139	3.125
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	125	3.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	100	2.5	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	79	1.975	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	78	1.95	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	62	1.55	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTC	39	0.975	RNA PCR Primer, Index 18 (100% over 44bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	37	0.9249999999999999	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	29	0.7250000000000001	RNA PCR Primer, Index 1 (97% over 35bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	16	0.4	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	16	0.4	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	15	0.375	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	14	0.35000000000000003	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	12	0.3	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	12	0.3	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	12	0.3	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	12	0.3	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC	10	0.25	RNA PCR Primer, Index 18 (100% over 50bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	10	0.25	RNA PCR Primer, Index 1 (100% over 32bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	10	0.25	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	10	0.25	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	9	0.22499999999999998	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	8	0.2	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	7	0.17500000000000002	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	7	0.17500000000000002	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	7	0.17500000000000002	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	7	0.17500000000000002	No Hit
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GGTCAATGATGACAACCCAATGTCATGAACAAAGGTGATGACATAAAATT	7	0.17500000000000002	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTAT	6	0.15	RNA PCR Primer, Index 18 (100% over 48bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (96% over 30bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ATCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAATTGTGGCTGGACATCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.4	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.55	0.0	0.0	0.0
4	0.0	0.65	0.0	0.0	0.0
5	0.0	0.7	0.0	0.0	0.0
6	0.0	0.8	0.0	0.0	0.0
7	0.0	1.9	0.0	0.0	0.0
8	0.0	2.1	0.0	0.0	0.0
9	0.0	2.175	0.0	0.0	0.0
10	0.0	2.3	0.0	0.0	0.0
11	0.0	2.475	0.0	0.0	0.0
12	0.0	2.7	0.0	0.0	0.0
13	0.0	2.925	0.0	0.0	0.0
14	0.0	3.225	0.0	0.0	0.0
15	0.0	3.675	0.0	0.0	0.0
16	0.0	5.375	0.0	0.0	0.0
17	0.0	6.075	0.0	0.0	0.0
18	0.0	7.45	0.0	0.0	0.0
19	0.0	8.275	0.0	0.0	0.0
20	0.0	9.125	0.0	0.0	0.0
21	0.0	14.875	0.0	0.0	0.0
22	0.0	24.875	0.0	0.0	0.0
23	0.0	51.925	0.0	0.0	0.0
24	0.0	62.925	0.0	0.0	0.0
25	0.0	68.4	0.0	0.0	0.0
26	0.0	69.9	0.0	0.0	0.0
27	0.0	70.7	0.0	0.0	0.0
28	0.0	71.125	0.0	0.0	0.0
29	0.0	71.65	0.0	0.0	0.0
30	0.0	72.0	0.0	0.0	0.0
31	0.0	72.925	0.0	0.0	0.0
32	0.0	73.9	0.0	0.0	0.0
33	0.0	74.775	0.0	0.0	0.0
34	0.0	76.825	0.0	0.0	0.0
35	0.0	77.8	0.0	0.0	0.0
36	0.0	78.425	0.0	0.0	0.0
37	0.0	79.175	0.0	0.0	0.0
38	0.0	79.675	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
GAGGTAG	20	6.952051E-4	44.000004	2
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AATCCAG	20	6.952051E-4	44.000004	8
CTGAGAC	20	6.952051E-4	44.000004	4
TAAACAT	20	6.952051E-4	44.000004	3
ATCCAGG	20	6.952051E-4	44.000004	9
AAACATC	20	6.952051E-4	44.000004	4
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
ATAGGCT	20	6.952051E-4	44.000004	16
CCTGAGA	20	6.952051E-4	44.000004	3
TCCAGGA	20	6.952051E-4	44.000004	10
GTGAGTT	20	6.952051E-4	44.000004	18
CTTGGTG	20	6.952051E-4	44.000004	16
GAGTTGG	20	6.952051E-4	44.000004	20
GGATAGG	20	6.952051E-4	44.000004	14
AGTAATC	20	6.952051E-4	44.000004	5
CTGGAAG	20	6.952051E-4	44.000004	16
>>END_MODULE
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238816 spots for SRR5030936.sra
Written 238816 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
Read 238806 spots for SRR5030936.sra
Written 238806 spots for SRR5030936.sra
SRR ids: ['SRR5030936.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ek8scypl
SRR5030936.sra spots: 2865682
blocks: [[1, 238806], [238807, 477612], [477613, 716418], [716419, 955224], [955225, 1194030], [1194031, 1432836], [1432837, 1671642], [1671643, 1910448], [1910449, 2149254], [2149255, 2388060], [2388061, 2626866], [2626867, 2865682]]
SRR5030936 file size 495608
SRR5030936 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030936 SRR5030936_1.fastq
Input file:	SRR5030936_1.fastq
trimmed:	SRR5030936-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:48:53 2020 >> started

Wed Mar  4 06:48:54 2020 >> done (1.757s)
2865682 reads processed; of these:
    161 ( 0.01%) short reads filtered out after trimming by size control
      7 ( 0.00%) empty reads filtered out after trimming by size control
2865514 (99.99%) reads available; of these:
 188631 ( 6.58%) trimmed reads available after processing
2676883 (93.42%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     62	  0.00%
 19	     56	  0.00%
 20	    150	  0.01%
 21	    162	  0.01%
 22	    279	  0.01%
 23	    332	  0.01%
 24	    372	  0.01%
 25	    446	  0.02%
 26	    400	  0.01%
 27	    565	  0.02%
 28	    675	  0.02%
 29	    977	  0.03%
 30	    821	  0.03%
 31	    987	  0.03%
 32	    987	  0.03%
 33	   1220	  0.04%
 34	   2800	  0.10%
 35	   2435	  0.08%
 36	   1843	  0.06%
 37	   2082	  0.07%
 38	   2434	  0.08%
 39	   2921	  0.10%
 40	   3361	  0.12%
 41	   3454	  0.12%
 42	   5085	  0.18%
 43	   6219	  0.22%
 44	  10928	  0.38%
 45	  13673	  0.48%
 46	  12951	  0.45%
 47	  18174	  0.63%
 48	  35388	  1.23%
 49	  56392	  1.97%
 50	2676883	 93.42%
2865514 reads passed initial QC


criterion=sequence-density
sequence-density=79.23
sequence-density-rank=1
fanout-score=18.88
fanout-score-rank=2
prefix-density=81.29
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC


criterion=fanout-score
sequence-density=1.47
sequence-density-rank=8
fanout-score=169.46
fanout-score-rank=1
prefix-density=80.45
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC -o SRR5030936 -
Input file:	STDIN
trimmed:	SRR5030936-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:49:08 2020 >> started

Wed Mar  4 06:49:12 2020 >> done (3.670s)
2793876 reads processed; of these:
 193288 ( 6.92%) short reads filtered out after trimming by size control
  15634 ( 0.56%) empty reads filtered out after trimming by size control
2584954 (92.52%) reads available; of these:
2268020 (87.74%) trimmed reads available after processing
 316934 (12.26%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  20992	  0.81%
 19	  23469	  0.91%
 20	 148785	  5.76%
 21	 289860	 11.21%
 22	 763422	 29.53%
 23	 304473	 11.78%
 24	 161511	  6.25%
 25	  45627	  1.77%
 26	  21682	  0.84%
 27	  17129	  0.66%
 28	  18797	  0.73%
 29	  13440	  0.52%
 30	  19829	  0.77%
 31	  33871	  1.31%
 32	  25162	  0.97%
 33	  65800	  2.55%
 34	  25694	  0.99%
 35	  21570	  0.83%
 36	  21907	  0.85%
 37	  14987	  0.58%
 38	  15914	  0.62%
 39	  16584	  0.64%
 40	  24257	  0.94%
 41	  30666	  1.19%
 42	  34692	  1.34%
 43	  25004	  0.97%
 44	  20675	  0.80%
 45	  27963	  1.08%
 46	  20111	  0.78%
 47	  14888	  0.58%
 48	   3566	  0.14%
 49	  13065	  0.51%
 50	 279562	 10.81%


criterion=sequence-density
sequence-density=5.41
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=11
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.53
sequence-density-rank=28
fanout-score=17.80
fanout-score-rank=1
prefix-density=9.45
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:49:32
                             Started mapping on |	Mar 04 06:49:32
                                    Finished on |	Mar 04 06:49:56
       Mapping speed, Million of reads per hour |	398.49

                          Number of input reads |	2656592
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1612928
                        Uniquely mapped reads % |	60.71%
                          Average mapped length |	27.47
                       Number of splices: Total |	1259
            Number of splices: Annotated (sjdb) |	218
                       Number of splices: GT/AG |	1171
                       Number of splices: GC/AG |	22
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	65
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	726194
             % of reads mapped to multiple loci |	27.34%
        Number of reads mapped to too many loci |	59867
             % of reads mapped to too many loci |	2.25%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.71%
                     % of reads unmapped: other |	0.98%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	317470	317470	317470
N_multimapping	726194	726194	726194
N_noFeature	74686	93950	1100865
N_ambiguous	897659	447417	14884
UnstrandedReadsAssigned:640583 PositiveStrandReadsAssigned:1071561 NegativeStrandReadsAssigned:497179
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030936 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030936-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,656,592 reads, 712,077 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,120 rounds

 109283 SRR5030936.ke.tsv
  52636 SRR5030936.se.tsv
 161919 total
==> SRR5030936.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030936.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030936 completed mapping pipeline successfully
