Starting /dee2/code/volunteer_pipeline.sh SRR5030902
    current disk space = 160660963328
    free memory = 73947180 
SRR5030902 SRAfilesize
21f56aa062630cc751ff05b497ff13e7  SRR5030902.sra
SRR5030902.sra file validated
SRR5030902 is single end
SRR5030902 is conventional basespace
SRR5030902 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5055	33.0	31.0	34.0	31.0	34.0
2	32.726	34.0	31.0	34.0	31.0	34.0
3	32.86375	34.0	31.0	34.0	31.0	34.0
4	36.2695	37.0	37.0	37.0	35.0	37.0
5	36.32725	37.0	37.0	37.0	35.0	37.0
6	36.083	37.0	35.0	37.0	35.0	37.0
7	36.1095	37.0	35.0	37.0	35.0	37.0
8	36.17	37.0	36.0	37.0	35.0	37.0
9	37.92575	39.0	38.0	39.0	35.0	39.0
10	38.02675	39.0	38.0	39.0	35.0	39.0
11	37.8865	39.0	38.0	39.0	35.0	39.0
12	37.84225	39.0	38.0	39.0	35.0	39.0
13	37.8145	39.0	38.0	39.0	35.0	39.0
14	39.318	41.0	39.0	41.0	36.0	41.0
15	39.374	41.0	39.0	41.0	36.0	41.0
16	39.2735	40.0	39.0	41.0	36.0	41.0
17	39.42975	41.0	39.0	41.0	36.0	41.0
18	39.31775	41.0	39.0	41.0	36.0	41.0
19	39.05125	40.0	39.0	41.0	36.0	41.0
20	39.1765	40.0	39.0	41.0	36.0	41.0
21	39.2165	41.0	39.0	41.0	36.0	41.0
22	39.24975	41.0	39.0	41.0	36.0	41.0
23	39.03925	40.0	39.0	41.0	36.0	41.0
24	38.71075	40.0	38.0	41.0	34.0	41.0
25	39.08	41.0	39.0	41.0	35.0	41.0
26	39.126	41.0	39.0	41.0	36.0	41.0
27	39.189	41.0	39.0	41.0	35.0	41.0
28	38.93475	40.0	39.0	41.0	35.0	41.0
29	38.5125	40.0	38.0	41.0	34.0	41.0
30	38.7665	40.0	38.0	41.0	34.0	41.0
31	38.9425	41.0	39.0	41.0	35.0	41.0
32	39.111	41.0	39.0	41.0	35.0	41.0
33	38.31225	40.0	38.0	41.0	34.0	41.0
34	38.2715	40.0	38.0	41.0	34.0	41.0
35	37.808	40.0	38.0	41.0	33.0	41.0
36	33.42075	38.0	30.0	40.0	18.0	41.0
37	35.5955	38.0	34.0	40.0	30.0	41.0
38	36.9505	39.0	37.0	40.0	31.0	41.0
39	36.64975	39.0	36.0	40.0	30.0	41.0
40	36.87875	39.0	37.0	40.0	30.0	41.0
41	37.23625	40.0	37.0	41.0	31.0	41.0
42	37.5275	40.0	38.0	41.0	33.0	41.0
43	37.22325	40.0	37.0	41.0	32.0	41.0
44	37.0435	40.0	37.0	41.0	31.0	41.0
45	36.586	39.0	36.0	41.0	30.0	41.0
46	34.682	38.0	34.0	40.0	24.0	41.0
47	35.72975	39.0	35.0	40.0	28.0	41.0
48	36.213	39.0	36.0	41.0	30.0	41.0
49	35.07575	39.0	35.0	40.0	27.0	41.0
50	30.644	34.0	28.0	39.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	1.0
18	2.0
19	4.0
20	6.0
21	7.0
22	7.0
23	6.0
24	13.0
25	17.0
26	20.0
27	26.0
28	28.0
29	36.0
30	60.0
31	68.0
32	68.0
33	117.0
34	166.0
35	176.0
36	294.0
37	484.0
38	975.0
39	1417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.5575575575575575	21.496496496496498	57.107107107107105	13.838838838838837
2	22.25	27.625	23.075000000000003	27.05
3	17.0	17.75	19.950000000000003	45.300000000000004
4	25.0	37.175000000000004	12.725	25.1
5	21.5	38.6	27.275	12.625
6	37.7	16.400000000000002	35.275	10.625
7	13.825000000000001	24.349999999999998	26.55	35.275
8	24.0	42.3	13.450000000000001	20.25
9	19.25	40.975	28.599999999999998	11.175
10	19.475	18.825	27.950000000000003	33.75
11	28.875	13.525	13.825000000000001	43.775
12	14.424999999999999	17.075000000000003	24.6	43.9
13	19.725	23.200000000000003	36.075	21.0
14	39.65	23.425	24.5	12.425
15	37.559389847461865	22.83070767691923	27.306826706676667	12.303075768942236
16	12.5	29.049999999999997	20.075000000000003	38.375
17	23.575	22.875	40.050000000000004	13.5
18	27.3	19.075	29.799999999999997	23.825
19	39.1	13.05	29.75	18.099999999999998
20	49.375	16.625	22.85	11.15
21	22.5	25.275	22.375	29.849999999999998
22	30.7	12.75	49.35	7.199999999999999
23	23.9	11.85	52.025	12.225
24	55.025	12.775	25.85	6.35
25	57.025000000000006	22.225	13.350000000000001	7.3999999999999995
26	27.025	51.975	11.625	9.375
27	12.825000000000001	55.35	22.725	9.1
28	8.649999999999999	25.3	53.800000000000004	12.25
29	8.35	10.75	62.625	18.275
30	8.375	5.25	37.574999999999996	48.8
31	12.049999999999999	4.5	49.475	33.975
32	24.525	4.875	22.85	47.75
33	59.199999999999996	5.375	11.4	24.025
34	70.075	6.05	11.924999999999999	11.95
35	68.025	6.925000000000001	16.325	8.725
36	43.0	7.425	38.7	10.875
37	48.325	8.674999999999999	21.6	21.4
38	23.05	12.5	11.475	52.97500000000001
39	12.875	22.175	7.475	57.475
40	11.65	53.925	8.9	25.525
41	22.125	57.525000000000006	7.775	12.575
42	54.75	30.0	8.025	7.225
43	57.875	27.175	6.925000000000001	8.025
44	29.049999999999997	53.65	6.425	10.875
45	14.149999999999999	57.425000000000004	10.775	17.65
46	8.825	27.775	18.6	44.800000000000004
47	6.75	12.6	43.125	37.525
48	7.5249999999999995	10.6	22.05	59.824999999999996
49	9.950000000000001	19.400000000000002	11.600000000000001	59.050000000000004
50	17.575	41.9	11.05	29.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	2.0
28	3.0
29	2.0
30	1.0
31	7.0
32	13.0
33	13.0
34	13.0
35	24.0
36	35.0
37	32.0
38	29.0
39	34.0
40	39.0
41	39.0
42	39.0
43	79.5
44	120.0
45	175.0
46	230.0
47	424.0
48	618.0
49	636.5
50	655.0
51	823.0
52	991.0
53	689.5
54	388.0
55	365.5
56	343.0
57	307.5
58	272.0
59	161.0
60	50.0
61	46.5
62	43.0
63	57.0
64	71.0
65	45.5
66	20.0
67	13.0
68	6.0
69	5.5
70	5.0
71	7.5
72	10.0
73	5.5
74	1.0
75	1.5
76	2.0
77	1.0
78	0.0
79	0.5
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10412440838404	27.400000000000002
2	9.668695064232589	7.1499999999999995
3	4.665314401622718	5.175
4	2.501690331304936	3.6999999999999997
5	1.757944557133198	3.25
6	1.2846517917511833	2.85
7	0.9465855307640297	2.45
8	0.5409060175794456	1.6
9	0.7437457741717377	2.475
>10	3.3806626098715347	23.75
>50	0.1352265043948614	3.175
>100	0.2704530087897228	17.025000000000002
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	252	6.3	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	160	4.0	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	145	3.6249999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	124	3.1	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	38	0.95	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	30	0.75	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 35bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTC	14	0.35000000000000003	RNA PCR Primer, Index 27 (100% over 44bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	12	0.3	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGC	11	0.27499999999999997	RNA PCR Primer, Index 27 (100% over 50bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTAT	9	0.22499999999999998	RNA PCR Primer, Index 27 (100% over 48bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	8	0.2	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	8	0.2	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	8	0.2	RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	6	0.15	No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	6	0.15	No Hit
TAAGGCACGCGGTGAATGAAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	6	0.15	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	5	0.125	RNA PCR Primer, Index 1 (100% over 34bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.3	0.0	0.0	0.0
2	0.0	0.3	0.0	0.0	0.0
3	0.0	0.55	0.0	0.0	0.0
4	0.0	0.55	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.6	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.3	0.0	0.0	0.0
9	0.0	1.375	0.0	0.0	0.0
10	0.0	1.6	0.0	0.0	0.0
11	0.0	1.7	0.0	0.0	0.0
12	0.0	1.825	0.0	0.0	0.0
13	0.0	2.075	0.0	0.0	0.0
14	0.0	2.275	0.0	0.0	0.0
15	0.0	2.525	0.0	0.0	0.0
16	0.0	3.125	0.0	0.0	0.0
17	0.0	3.825	0.0	0.0	0.0
18	0.0	4.6	0.0	0.0	0.0
19	0.0	5.3	0.0	0.0	0.0
20	0.0	6.425	0.0	0.0	0.0
21	0.0	10.95	0.0	0.0	0.0
22	0.0	22.475	0.0	0.0	0.0
23	0.0	58.5	0.0	0.0	0.0
24	0.0	73.775	0.0	0.0	0.0
25	0.0	78.85	0.0	0.0	0.0
26	0.0	79.825	0.0	0.0	0.0
27	0.0	80.2	0.0	0.0	0.0
28	0.0	80.525	0.0	0.0	0.0
29	0.0	80.8	0.0	0.0	0.0
30	0.0	81.025	0.0	0.0	0.0
31	0.0	81.625	0.0	0.0	0.0
32	0.0	82.875	0.0	0.0	0.0
33	0.0	83.775	0.0	0.0	0.0
34	0.0	85.3	0.0	0.0	0.0
35	0.0	85.925	0.0	0.0	0.0
36	0.0	86.325	0.0	0.0	0.0
37	0.0	86.775	0.0	0.0	0.0
38	0.0	87.1	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACT	20	6.952051E-4	44.000004	11
ATCCTCG	20	6.952051E-4	44.000004	8
GAGGTAG	20	6.952051E-4	44.000004	2
TCCTCGA	20	6.952051E-4	44.000004	9
AAGTAAT	45	2.728484E-10	44.000004	4
TCAAGTA	45	2.728484E-10	44.000004	2
AATCCAG	45	2.728484E-10	44.000004	8
TCGACTG	20	6.952051E-4	44.000004	12
ATCCAGG	45	2.728484E-10	44.000004	9
GAGTGGA	20	6.952051E-4	44.000004	20
TTCAAGT	45	2.728484E-10	44.000004	1
CGGGGGC	20	6.952051E-4	44.000004	32
AGGATAG	40	5.315087E-9	44.000004	13
CATCCTC	20	6.952051E-4	44.000004	7
GAAGCTT	20	6.952051E-4	44.000004	19
ATAGGCT	40	5.315087E-9	44.000004	16
ACCCGTA	20	6.952051E-4	44.000004	2
TCCAGGA	45	2.728484E-10	44.000004	10
GTGAGTG	20	6.952051E-4	44.000004	18
ACATCCT	40	5.315087E-9	44.000004	6
>>END_MODULE
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280629 spots for SRR5030902.sra
Written 280629 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
Read 280618 spots for SRR5030902.sra
Written 280618 spots for SRR5030902.sra
SRR ids: ['SRR5030902.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6rggf6eh
SRR5030902.sra spots: 3367427
blocks: [[1, 280618], [280619, 561236], [561237, 841854], [841855, 1122472], [1122473, 1403090], [1403091, 1683708], [1683709, 1964326], [1964327, 2244944], [2244945, 2525562], [2525563, 2806180], [2806181, 3086798], [3086799, 3367427]]
SRR5030902 file size 582574
SRR5030902 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030902 SRR5030902_1.fastq
Input file:	SRR5030902_1.fastq
trimmed:	SRR5030902-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:40:44 2020 >> started

Wed Mar  4 06:40:46 2020 >> done (2.100s)
3367427 reads processed; of these:
    128 ( 0.00%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
3367296 (100.00%) reads available; of these:
 173113 ( 5.14%) trimmed reads available after processing
3194183 (94.86%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     36	  0.00%
 19	     44	  0.00%
 20	    119	  0.00%
 21	    152	  0.00%
 22	    276	  0.01%
 23	    336	  0.01%
 24	    307	  0.01%
 25	    394	  0.01%
 26	    308	  0.01%
 27	    476	  0.01%
 28	    600	  0.02%
 29	   1048	  0.03%
 30	    653	  0.02%
 31	    845	  0.03%
 32	    815	  0.02%
 33	   1151	  0.03%
 34	   3302	  0.10%
 35	   2811	  0.08%
 36	   1950	  0.06%
 37	   2042	  0.06%
 38	   2163	  0.06%
 39	   2283	  0.07%
 40	   2464	  0.07%
 41	   3093	  0.09%
 42	   4459	  0.13%
 43	   5623	  0.17%
 44	  11270	  0.33%
 45	  12595	  0.37%
 46	  10259	  0.30%
 47	  15568	  0.46%
 48	  38210	  1.13%
 49	  47461	  1.41%
 50	3194183	 94.86%
3367296 reads passed initial QC


criterion=sequence-density
sequence-density=87.06
sequence-density-rank=1
fanout-score=17.45
fanout-score-rank=1
prefix-density=88.43
prefix-fanout=17.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC


criterion=fanout-score
sequence-density=87.06
sequence-density-rank=1
fanout-score=17.45
fanout-score-rank=1
prefix-density=88.43
prefix-fanout=17.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC -o SRR5030902 -
Input file:	STDIN
trimmed:	SRR5030902-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:41:01 2020 >> started

Wed Mar  4 06:41:06 2020 >> done (4.262s)
3290767 reads processed; of these:
 144464 ( 4.39%) short reads filtered out after trimming by size control
  12173 ( 0.37%) empty reads filtered out after trimming by size control
3134130 (95.24%) reads available; of these:
2910551 (92.87%) trimmed reads available after processing
 223579 ( 7.13%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  22659	  0.72%
 19	  37551	  1.20%
 20	 166195	  5.30%
 21	 381910	 12.19%
 22	1154706	 36.84%
 23	 521190	 16.63%
 24	 188495	  6.01%
 25	  31054	  0.99%
 26	  14884	  0.47%
 27	  14371	  0.46%
 28	  15563	  0.50%
 29	  12155	  0.39%
 30	  22312	  0.71%
 31	  29901	  0.95%
 32	  26522	  0.85%
 33	  48565	  1.55%
 34	  23884	  0.76%
 35	  17304	  0.55%
 36	  18997	  0.61%
 37	  11160	  0.36%
 38	  12820	  0.41%
 39	  12179	  0.39%
 40	  15623	  0.50%
 41	  22572	  0.72%
 42	  25227	  0.80%
 43	  18825	  0.60%
 44	  16006	  0.51%
 45	  17957	  0.57%
 46	  13710	  0.44%
 47	  10925	  0.35%
 48	   2447	  0.08%
 49	   8452	  0.27%
 50	 198009	  6.32%


criterion=sequence-density
sequence-density=9.20
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.43
sequence-density-rank=32
fanout-score=16.28
fanout-score-rank=1
prefix-density=6.93
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:41:26
                             Started mapping on |	Mar 04 06:41:27
                                    Finished on |	Mar 04 06:41:52
       Mapping speed, Million of reads per hour |	462.33

                          Number of input reads |	3210659
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1663297
                        Uniquely mapped reads % |	51.81%
                          Average mapped length |	25.60
                       Number of splices: Total |	1266
            Number of splices: Annotated (sjdb) |	156
                       Number of splices: GT/AG |	1150
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	103
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.51
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1278388
             % of reads mapped to multiple loci |	39.82%
        Number of reads mapped to too many loci |	59102
             % of reads mapped to too many loci |	1.84%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.98%
                     % of reads unmapped: other |	0.56%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	268974	268974	268974
N_multimapping	1278388	1278388	1278388
N_noFeature	69695	81790	1272016
N_ambiguous	915236	581429	12273
UnstrandedReadsAssigned:678366 PositiveStrandReadsAssigned:1000078 NegativeStrandReadsAssigned:379008
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030902 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030902-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,210,659 reads, 682,167 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,126 rounds

 109283 SRR5030902.ke.tsv
  52636 SRR5030902.se.tsv
 161919 total
==> SRR5030902.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030902.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030902 completed mapping pipeline successfully
