Starting /dee2/code/volunteer_pipeline.sh SRR5030861
    current disk space = 160654934016
    free memory = 73958932 
SRR5030861 SRAfilesize
a24605bfe884116b9a2a7c5dfcbacfef  SRR5030861.sra
SRR5030861.sra file validated
SRR5030861 is single end
SRR5030861 is conventional basespace
SRR5030861 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39225	33.0	31.0	34.0	31.0	34.0
2	32.7095	34.0	31.0	34.0	31.0	34.0
3	32.6765	34.0	31.0	34.0	31.0	34.0
4	36.22075	37.0	37.0	37.0	35.0	37.0
5	36.30725	37.0	37.0	37.0	35.0	37.0
6	36.03075	37.0	35.0	37.0	35.0	37.0
7	36.07575	37.0	35.0	37.0	35.0	37.0
8	36.15275	37.0	36.0	37.0	35.0	37.0
9	37.95525	39.0	38.0	39.0	35.0	39.0
10	37.8875	39.0	38.0	39.0	35.0	39.0
11	37.779	39.0	38.0	39.0	35.0	39.0
12	37.77925	39.0	38.0	39.0	35.0	39.0
13	37.73625	39.0	38.0	39.0	35.0	39.0
14	39.077	40.0	39.0	41.0	36.0	41.0
15	39.15525	41.0	39.0	41.0	36.0	41.0
16	39.12025	40.0	38.0	41.0	36.0	41.0
17	39.0015	40.0	38.0	41.0	36.0	41.0
18	39.108	41.0	39.0	41.0	35.0	41.0
19	39.0885	41.0	39.0	41.0	35.0	41.0
20	38.90075	40.0	38.0	41.0	35.0	41.0
21	38.861	40.0	38.0	41.0	34.0	41.0
22	39.07825	40.0	39.0	41.0	36.0	41.0
23	38.97675	40.0	39.0	41.0	35.0	41.0
24	38.06275	40.0	38.0	41.0	32.0	41.0
25	38.3515	40.0	38.0	41.0	34.0	41.0
26	38.42425	40.0	38.0	41.0	34.0	41.0
27	38.54425	40.0	38.0	41.0	34.0	41.0
28	38.327	40.0	38.0	41.0	34.0	41.0
29	37.76875	40.0	38.0	41.0	31.0	41.0
30	37.92175	40.0	37.0	41.0	33.0	41.0
31	38.264	40.0	38.0	41.0	33.0	41.0
32	38.3485	40.0	38.0	41.0	34.0	41.0
33	37.272	40.0	37.0	41.0	31.0	41.0
34	37.274	40.0	37.0	41.0	30.0	41.0
35	37.3615	39.0	37.0	41.0	31.0	41.0
36	33.64125	38.0	30.0	40.0	21.0	41.0
37	35.799	38.0	34.0	40.0	30.0	41.0
38	37.066	39.0	37.0	40.0	32.0	41.0
39	37.141	39.0	37.0	40.0	32.0	41.0
40	36.9165	39.0	37.0	40.0	31.0	41.0
41	36.635	39.0	36.0	40.0	30.0	41.0
42	36.648	39.0	36.0	40.0	30.0	41.0
43	36.76675	39.0	36.0	41.0	31.0	41.0
44	36.94575	39.0	36.0	41.0	32.0	41.0
45	36.48475	39.0	36.0	41.0	31.0	41.0
46	34.09175	38.0	33.0	40.0	24.0	41.0
47	34.9285	38.0	34.0	40.0	26.0	41.0
48	34.83775	38.0	34.0	40.0	27.0	41.0
49	34.32475	38.0	34.0	40.0	24.0	41.0
50	30.332	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	2.0
17	1.0
18	3.0
19	10.0
20	5.0
21	4.0
22	10.0
23	8.0
24	12.0
25	23.0
26	23.0
27	28.0
28	42.0
29	49.0
30	63.0
31	82.0
32	88.0
33	127.0
34	139.0
35	220.0
36	321.0
37	560.0
38	1041.0
39	1136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.472945891783567	24.9248496993988	46.668336673346694	16.93386773547094
2	23.375	26.05	19.425	31.15
3	23.95598899724931	21.080270067516878	23.305826456614152	31.657914478619652
4	29.849999999999998	26.450000000000003	16.55	27.150000000000002
5	21.075	41.949999999999996	23.599999999999998	13.375
6	33.6	17.05	34.225	15.125
7	19.325	19.375	25.374999999999996	35.925000000000004
8	26.85	27.975	15.950000000000001	29.225
9	20.474999999999998	32.85	30.349999999999998	16.325
10	24.25	21.675	29.25	24.825
11	30.675	14.95	16.150000000000002	38.224999999999994
12	15.950000000000001	22.525000000000002	28.799999999999997	32.725
13	30.15	19.0	29.675	21.175
14	28.075	31.4	22.025	18.5
15	32.25	24.4	27.375	15.975
16	18.5	21.95	22.925	36.625
17	23.025000000000002	22.15	39.1	15.725
18	23.474999999999998	17.9	31.05	27.575
19	34.375	15.125	31.35	19.15
20	37.675	17.625	28.575	16.125
21	23.724999999999998	19.950000000000003	27.700000000000003	28.625
22	28.325	14.649999999999999	43.025000000000006	14.000000000000002
23	28.025	14.149999999999999	38.725	19.1
24	48.15	16.575	24.325	10.95
25	44.425	23.775	16.45	15.35
26	28.275	41.75	15.15	14.825
27	19.275000000000002	43.2	22.775000000000002	14.75
28	14.000000000000002	25.05	43.5	17.45
29	11.725	16.175	52.275000000000006	19.825
30	11.35	10.35	33.875	44.425
31	15.825	7.425	44.800000000000004	31.95
32	29.2	8.674999999999999	21.05	41.075
33	48.825	12.375	15.975	22.825
34	53.425	10.174999999999999	18.575	17.825
35	57.62500000000001	8.875	19.7	13.8
36	43.225	10.5	30.55	15.725
37	42.025	12.174999999999999	21.475	24.325
38	23.200000000000003	18.0	15.0	43.8
39	18.55	24.0	12.425	45.025
40	18.425	42.699999999999996	15.475	23.400000000000002
41	25.8	43.2	12.575	18.425
42	46.800000000000004	27.950000000000003	13.775	11.475
43	45.15	30.575000000000003	12.7	11.575000000000001
44	31.6	44.85	9.675	13.875000000000002
45	21.075	45.7	15.325	17.9
46	15.049999999999999	27.500000000000004	20.3	37.15
47	12.475	18.325	37.275000000000006	31.924999999999997
48	14.174999999999999	13.65	21.025	51.15
49	16.2	18.975	17.0	47.825
50	19.75	32.725	16.025	31.5
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	2.0
14	3.0
15	2.0
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	2.0
28	3.0
29	6.0
30	9.0
31	18.5
32	28.0
33	31.5
34	35.0
35	45.5
36	56.0
37	75.0
38	94.0
39	84.5
40	75.0
41	62.5
42	50.0
43	89.5
44	129.0
45	175.0
46	221.0
47	297.0
48	373.0
49	479.0
50	585.0
51	680.5
52	776.0
53	601.5
54	427.0
55	421.5
56	416.0
57	345.5
58	275.0
59	179.5
60	84.0
61	62.5
62	41.0
63	82.5
64	124.0
65	81.0
66	38.0
67	28.5
68	19.0
69	29.5
70	40.0
71	54.5
72	69.0
73	39.0
74	9.0
75	5.0
76	1.0
77	1.0
78	1.0
79	6.0
80	11.0
81	7.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52533992583437	30.55
2	10.074165636588381	8.15
3	3.4610630407911	4.2
4	2.2867737948084055	3.6999999999999997
5	1.6687268232385661	3.375
6	1.1742892459826948	2.85
7	0.6180469715698393	1.7500000000000002
8	0.5562422744128553	1.7999999999999998
9	0.3708281829419036	1.35
>10	3.7700865265760197	27.425
>50	0.4326328800988875	12.2
>100	0.061804697156983925	2.65
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	106	2.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	81	2.025	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	73	1.825	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	72	1.7999999999999998	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	65	1.625	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	63	1.575	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	40	1.0	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	31	0.775	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	27	0.675	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	26	0.65	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC	25	0.625	RNA PCR Primer, Index 5 (97% over 35bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	24	0.6	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	23	0.575	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	20	0.5	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (96% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	16	0.4	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC	15	0.375	RNA PCR Primer, Index 16 (100% over 44bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	15	0.375	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	14	0.35000000000000003	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	13	0.325	No Hit
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	13	0.325	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	12	0.3	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	12	0.3	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	12	0.3	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	11	0.27499999999999997	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	11	0.27499999999999997	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	10	0.25	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	10	0.25	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	10	0.25	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC	10	0.25	RNA PCR Primer, Index 16 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	9	0.22499999999999998	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	9	0.22499999999999998	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	8	0.2	No Hit
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	8	0.2	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAG	8	0.2	No Hit
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	8	0.2	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	7	0.17500000000000002	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	7	0.17500000000000002	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	7	0.17500000000000002	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAAT	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	6	0.15	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CAATTCTGAGTCTTAACCCAATTTTTTGAGGTCCTGCTCGTGCAGGGCTT	6	0.15	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TACCATGATGATAACATAGTTCAGCAGACTTAACTCTGATGAACAATCAT	5	0.125	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	5	0.125	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	5	0.125	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAAGGTGATGACATAAAATTCATGCTCAATAGGATTACGCTGAGGTGGAA	5	0.125	No Hit
GCAGCTCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTT	5	0.125	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTAT	5	0.125	RNA PCR Primer, Index 16 (100% over 48bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GATACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTCCCGGGCGGG	5	0.125	No Hit
AGCTACATCTGGCTACTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.325	0.0	0.0	0.0
2	0.0	0.325	0.0	0.0	0.0
3	0.0	0.475	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.6	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.3	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.475	0.0	0.0	0.0
11	0.0	1.575	0.0	0.0	0.0
12	0.0	1.85	0.0	0.0	0.0
13	0.0	2.025	0.0	0.0	0.0
14	0.0	2.125	0.0	0.0	0.0
15	0.0	2.3	0.0	0.0	0.0
16	0.0	3.325	0.0	0.0	0.0
17	0.0	3.65	0.0	0.0	0.0
18	0.0	4.3	0.0	0.0	0.0
19	0.0	4.775	0.0	0.0	0.0
20	0.0	5.5	0.0	0.0	0.0
21	0.0	10.425	0.0	0.0	0.0
22	0.0	20.175	0.0	0.0	0.0
23	0.0	44.4	0.0	0.0	0.0
24	0.0	54.45	0.0	0.0	0.0
25	0.0	60.35	0.0	0.0	0.0
26	0.0	61.9	0.0	0.0	0.0
27	0.0	62.575	0.0	0.0	0.0
28	0.0	62.9	0.0	0.0	0.0
29	0.0	63.275	0.0	0.0	0.0
30	0.0	63.75	0.0	0.0	0.0
31	0.0	64.275	0.0	0.0	0.0
32	0.0	65.225	0.0	0.0	0.0
33	0.0	66.3	0.0	0.0	0.0
34	0.0	69.325	0.0	0.0	0.0
35	0.0	70.475	0.0	0.0	0.0
36	0.0	71.325	0.0	0.0	0.0
37	0.0	72.325	0.0	0.0	0.0
38	0.0	72.875	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTTG	20	6.952051E-4	44.000004	18
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
AACCGGT	20	6.952051E-4	44.000004	9
AACTGTT	20	6.952051E-4	44.000004	17
GAACCGG	20	6.952051E-4	44.000004	8
GTCTCTT	20	6.952051E-4	44.000004	14
AGTGAAC	20	6.952051E-4	44.000004	5
CGGTCTC	20	6.952051E-4	44.000004	12
CTGTTGG	20	6.952051E-4	44.000004	19
TGAACCG	20	6.952051E-4	44.000004	7
CACAGTG	20	6.952051E-4	44.000004	2
CAGTGAA	20	6.952051E-4	44.000004	4
CCGGTCT	20	6.952051E-4	44.000004	11
TGATGAA	25	3.6958358E-5	44.0	6
GTTGAAG	25	3.6958358E-5	44.0	10
CAGTTGA	25	3.6958358E-5	44.0	8
CTGAGCT	25	3.6958358E-5	44.0	11
CCAGTTG	25	3.6958358E-5	44.0	7
ATGATGA	35	1.02281774E-7	44.0	5
>>END_MODULE
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182799 spots for SRR5030861.sra
Written 182799 spots for SRR5030861.sra
Read 182807 spots for SRR5030861.sra
Written 182807 spots for SRR5030861.sra
SRR ids: ['SRR5030861.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_asqqccx4
SRR5030861.sra spots: 2193596
blocks: [[1, 182799], [182800, 365598], [365599, 548397], [548398, 731196], [731197, 913995], [913996, 1096794], [1096795, 1279593], [1279594, 1462392], [1462393, 1645191], [1645192, 1827990], [1827991, 2010789], [2010790, 2193596]]
SRR5030861 file size 379118
SRR5030861 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030861 SRR5030861_1.fastq
Input file:	SRR5030861_1.fastq
trimmed:	SRR5030861-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:31:01 2020 >> started

Wed Mar  4 06:31:02 2020 >> done (1.258s)
2193596 reads processed; of these:
    118 ( 0.01%) short reads filtered out after trimming by size control
      1 ( 0.00%) empty reads filtered out after trimming by size control
2193477 (99.99%) reads available; of these:
 175722 ( 8.01%) trimmed reads available after processing
2017755 (91.99%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     33	  0.00%
 19	     44	  0.00%
 20	    122	  0.01%
 21	    147	  0.01%
 22	    197	  0.01%
 23	    281	  0.01%
 24	    359	  0.02%
 25	    440	  0.02%
 26	    343	  0.02%
 27	    451	  0.02%
 28	    595	  0.03%
 29	    784	  0.04%
 30	    710	  0.03%
 31	    840	  0.04%
 32	   1115	  0.05%
 33	   1729	  0.08%
 34	   4576	  0.21%
 35	   2451	  0.11%
 36	   1819	  0.08%
 37	   2106	  0.10%
 38	   3052	  0.14%
 39	   3141	  0.14%
 40	   4350	  0.20%
 41	   3583	  0.16%
 42	   5751	  0.26%
 43	   4376	  0.20%
 44	  10734	  0.49%
 45	  12183	  0.56%
 46	  12343	  0.56%
 47	  14660	  0.67%
 48	  33469	  1.53%
 49	  48938	  2.23%
 50	2017755	 91.99%
2193477 reads passed initial QC


criterion=sequence-density
sequence-density=71.75
sequence-density-rank=1
fanout-score=22.07
fanout-score-rank=2
prefix-density=74.54
prefix-fanout=21.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC


criterion=fanout-score
sequence-density=1.88
sequence-density-rank=7
fanout-score=120.62
fanout-score-rank=1
prefix-density=74.00
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC -o SRR5030861 -
Input file:	STDIN
trimmed:	SRR5030861-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:31:14 2020 >> started

Wed Mar  4 06:31:16 2020 >> done (2.303s)
2132547 reads processed; of these:
  93070 ( 4.36%) short reads filtered out after trimming by size control
   6035 ( 0.28%) empty reads filtered out after trimming by size control
2033442 (95.35%) reads available; of these:
1692100 (83.21%) trimmed reads available after processing
 341342 (16.79%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   9800	  0.48%
 19	  17550	  0.86%
 20	  96034	  4.72%
 21	 214567	 10.55%
 22	 518383	 25.49%
 23	 227040	 11.17%
 24	 112646	  5.54%
 25	  30659	  1.51%
 26	  16202	  0.80%
 27	  12309	  0.61%
 28	  13663	  0.67%
 29	  10163	  0.50%
 30	  15371	  0.76%
 31	  19050	  0.94%
 32	  23268	  1.14%
 33	  60049	  2.95%
 34	  24191	  1.19%
 35	  20424	  1.00%
 36	  25128	  1.24%
 37	  12598	  0.62%
 38	  13763	  0.68%
 39	  15080	  0.74%
 40	  23220	  1.14%
 41	  31927	  1.57%
 42	  37550	  1.85%
 43	  27074	  1.33%
 44	  22056	  1.08%
 45	  29257	  1.44%
 46	  22603	  1.11%
 47	  18539	  0.91%
 48	   3859	  0.19%
 49	  10088	  0.50%
 50	 299331	 14.72%


criterion=sequence-density
sequence-density=4.70
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=35
fanout-score=36.76
fanout-score-rank=1
prefix-density=0.99
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:31:37
                             Started mapping on |	Mar 04 06:31:37
                                    Finished on |	Mar 04 06:31:58
       Mapping speed, Million of reads per hour |	359.04

                          Number of input reads |	2094372
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1184715
                        Uniquely mapped reads % |	56.57%
                          Average mapped length |	29.42
                       Number of splices: Total |	1014
            Number of splices: Annotated (sjdb) |	240
                       Number of splices: GT/AG |	930
                       Number of splices: GC/AG |	19
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	63
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.45
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.10
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	593299
             % of reads mapped to multiple loci |	28.33%
        Number of reads mapped to too many loci |	55666
             % of reads mapped to too many loci |	2.66%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.82%
                     % of reads unmapped: other |	0.63%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	316358	316358	316358
N_multimapping	593299	593299	593299
N_noFeature	59550	76845	861859
N_ambiguous	640653	366675	16729
UnstrandedReadsAssigned:484512 PositiveStrandReadsAssigned:741195 NegativeStrandReadsAssigned:306127
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030861 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030861-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,094,372 reads, 519,837 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,127 rounds

 109283 SRR5030861.ke.tsv
  52636 SRR5030861.se.tsv
 161919 total
==> SRR5030861.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030861.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030861 completed mapping pipeline successfully
