Starting /dee2/code/volunteer_pipeline.sh SRR5031058
    current disk space = 160510427136
    free memory = 73953916 
SRR5031058 SRAfilesize
05f50cf25b741a1c4a55228c1bb9c9b7  SRR5031058.sra
SRR5031058.sra file validated
SRR5031058 is single end
SRR5031058 is conventional basespace
SRR5031058 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031058_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24175	33.0	31.0	34.0	30.0	34.0
2	32.471	34.0	31.0	34.0	31.0	34.0
3	32.4845	34.0	31.0	34.0	30.0	34.0
4	36.09325	37.0	35.0	37.0	35.0	37.0
5	35.96675	37.0	35.0	37.0	35.0	37.0
6	35.6295	37.0	35.0	37.0	33.0	37.0
7	35.748	37.0	35.0	37.0	33.0	37.0
8	35.65325	37.0	35.0	37.0	33.0	37.0
9	37.31	39.0	37.0	39.0	34.0	39.0
10	37.61025	39.0	37.0	39.0	35.0	39.0
11	37.62825	39.0	37.0	39.0	35.0	39.0
12	37.53825	39.0	37.0	39.0	35.0	39.0
13	37.59075	39.0	37.0	39.0	35.0	39.0
14	39.0015	40.0	38.0	41.0	35.0	41.0
15	38.89925	40.0	38.0	41.0	35.0	41.0
16	38.86175	40.0	38.0	41.0	35.0	41.0
17	38.72975	40.0	38.0	41.0	34.0	41.0
18	38.86125	40.0	38.0	41.0	34.0	41.0
19	38.55025	40.0	38.0	41.0	34.0	41.0
20	38.84525	40.0	38.0	41.0	34.0	41.0
21	38.742	40.0	38.0	41.0	34.0	41.0
22	38.91075	40.0	39.0	41.0	35.0	41.0
23	38.693	40.0	38.0	41.0	34.0	41.0
24	38.7905	40.0	38.0	41.0	35.0	41.0
25	39.0305	41.0	39.0	41.0	35.0	41.0
26	38.5895	40.0	38.0	41.0	34.0	41.0
27	38.6615	40.0	38.0	41.0	34.0	41.0
28	38.62675	40.0	38.0	41.0	34.0	41.0
29	38.32475	40.0	38.0	41.0	34.0	41.0
30	38.20025	40.0	38.0	41.0	33.0	41.0
31	38.44525	40.0	38.0	41.0	34.0	41.0
32	38.34075	40.0	38.0	41.0	33.0	41.0
33	38.41925	40.0	38.0	41.0	34.0	41.0
34	38.5435	40.0	38.0	41.0	34.0	41.0
35	38.3655	40.0	38.0	41.0	34.0	41.0
36	37.99375	40.0	38.0	41.0	33.0	41.0
37	37.58575	40.0	37.0	41.0	32.0	41.0
38	36.875	39.0	36.0	41.0	30.0	41.0
39	37.3335	39.0	37.0	41.0	31.0	41.0
40	37.56575	40.0	37.0	41.0	33.0	41.0
41	37.73875	40.0	38.0	41.0	33.0	41.0
42	37.96775	40.0	38.0	41.0	33.0	41.0
43	38.2955	40.0	38.0	41.0	34.0	41.0
44	37.886	40.0	38.0	41.0	33.0	41.0
45	37.69675	40.0	38.0	41.0	33.0	41.0
46	37.5345	40.0	37.0	41.0	33.0	41.0
47	37.30025	40.0	37.0	41.0	31.0	41.0
48	36.92375	40.0	36.0	41.0	31.0	41.0
49	37.1105	40.0	37.0	41.0	31.0	41.0
50	36.6485	40.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	1.0
16	1.0
17	2.0
18	2.0
19	3.0
20	2.0
21	3.0
22	3.0
23	7.0
24	8.0
25	16.0
26	14.0
27	19.0
28	37.0
29	37.0
30	38.0
31	62.0
32	79.0
33	131.0
34	148.0
35	191.0
36	259.0
37	432.0
38	662.0
39	1839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.649999999999999	22.6	55.75	13.0
2	21.183253948357986	28.02707445475057	23.31411381298571	27.475557783905742
3	17.4	19.950000000000003	17.599999999999998	45.050000000000004
4	24.675	35.925000000000004	13.725000000000001	25.674999999999997
5	22.6	36.6	28.525	12.275
6	39.225	13.175	35.225	12.375
7	13.725000000000001	25.35	26.650000000000002	34.275
8	24.5	40.725	15.174999999999999	19.6
9	19.3	39.574999999999996	27.900000000000002	13.225000000000001
10	19.2	21.325	26.875	32.6
11	30.325000000000003	10.525	16.35	42.8
12	15.075	15.375	24.2	45.35
13	20.45	22.55	37.724999999999994	19.275000000000002
14	38.5	24.675	24.275	12.55
15	39.65	21.575	27.925	10.85
16	13.0	28.625	22.575	35.8
17	24.7	23.7	38.3	13.3
18	27.05	20.45	28.349999999999998	24.15
19	39.125	12.975	30.125	17.775
20	50.74999999999999	16.1	22.85	10.299999999999999
21	22.7	24.125	23.599999999999998	29.575000000000003
22	30.4	12.45	49.925000000000004	7.225
23	23.325000000000003	12.75	51.824999999999996	12.1
24	53.2	11.899999999999999	28.575	6.325
25	57.9	20.724999999999998	14.575	6.800000000000001
26	28.999999999999996	50.775000000000006	11.1	9.125
27	13.15	55.50000000000001	22.675	8.674999999999999
28	8.6	26.424999999999997	52.2	12.775
29	9.3	11.25	61.625	17.825
30	7.9	5.375	38.9	47.825
31	11.774999999999999	3.8249999999999997	49.475	34.925
32	23.3	4.9750000000000005	24.075	47.65
33	57.95	5.675	12.8	23.575
34	70.19999999999999	4.825	11.475	13.5
35	69.525	6.3	17.299999999999997	6.875000000000001
36	42.175000000000004	6.45	40.300000000000004	11.075
37	48.699999999999996	7.925	22.75	20.625
38	24.9	12.475	11.1	51.525
39	13.925	21.175	8.175	56.725
40	12.275	51.949999999999996	7.775	28.000000000000004
41	21.375	57.35	6.9750000000000005	14.299999999999999
42	52.225	31.275	8.5	8.0
43	59.35	25.4	6.625	8.625
44	31.324999999999996	51.6	5.925	11.15
45	16.525000000000002	57.025000000000006	9.85	16.6
46	10.525	27.3	16.275000000000002	45.9
47	8.375	12.625	41.425	37.574999999999996
48	8.375	9.85	22.05	59.724999999999994
49	10.549999999999999	17.525	11.525	60.4
50	16.025	41.349999999999994	9.9	32.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	2.5
30	5.0
31	5.0
32	5.0
33	7.0
34	9.0
35	11.5
36	14.0
37	16.0
38	18.0
39	22.0
40	26.0
41	33.5
42	41.0
43	77.0
44	113.0
45	179.5
46	246.0
47	446.0
48	646.0
49	635.0
50	624.0
51	813.0
52	1002.0
53	671.5
54	341.0
55	351.0
56	361.0
57	324.0
58	287.0
59	187.5
60	88.0
61	60.0
62	32.0
63	43.5
64	55.0
65	39.0
66	23.0
67	17.5
68	12.0
69	14.5
70	17.0
71	22.0
72	27.0
73	14.0
74	1.0
75	1.0
76	1.0
77	1.0
78	1.0
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.27499999999999997
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.68870523415978	27.474999999999998
2	9.43526170798898	6.8500000000000005
3	3.8567493112947657	4.2
4	2.5482093663911844	3.6999999999999997
5	1.6528925619834711	3.0
6	0.8264462809917356	1.7999999999999998
7	0.7575757575757576	1.925
8	0.6887052341597797	2.0
9	0.6198347107438017	2.025
>10	3.512396694214876	25.4
>50	0.13774104683195593	3.5999999999999996
>100	0.27548209366391185	18.025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	286	7.1499999999999995	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	170	4.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	150	3.75	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	63	1.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	39	0.975	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	35	0.8750000000000001	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	31	0.775	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGC	16	0.4	RNA PCR Primer, Index 30 (100% over 50bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	11	0.27499999999999997	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	10	0.25	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC	8	0.2	RNA PCR Primer, Index 30 (100% over 44bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	6	0.15	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACAGAAATATTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGCTACACATTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	5	0.125	RNA PCR Primer, Index 7 (100% over 35bp)
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCAGGCTCAGTCCCCTCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.425	0.0	0.0	0.0
2	0.0	0.425	0.0	0.0	0.0
3	0.0	0.5	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.5	0.0	0.0	0.0
6	0.0	0.575	0.0	0.0	0.0
7	0.0	0.85	0.0	0.0	0.0
8	0.0	0.95	0.0	0.0	0.0
9	0.0	0.95	0.0	0.0	0.0
10	0.0	1.075	0.0	0.0	0.0
11	0.0	1.325	0.0	0.0	0.0
12	0.0	1.4	0.0	0.0	0.0
13	0.0	1.525	0.0	0.0	0.0
14	0.0	1.8	0.0	0.0	0.0
15	0.0	2.125	0.0	0.0	0.0
16	0.0	2.55	0.0	0.0	0.0
17	0.0	3.125	0.0	0.0	0.0
18	0.0	3.9	0.0	0.0	0.0
19	0.0	4.375	0.0	0.0	0.0
20	0.0	5.375	0.0	0.0	0.0
21	0.0	9.75	0.0	0.0	0.0
22	0.0	21.05	0.0	0.0	0.0
23	0.0	56.225	0.0	0.0	0.0
24	0.0	72.925	0.0	0.0	0.0
25	0.0	78.85	0.0	0.0	0.0
26	0.0	79.925	0.0	0.0	0.0
27	0.0	80.6	0.0	0.0	0.0
28	0.0	81.0	0.0	0.0	0.0
29	0.0	81.65	0.0	0.0	0.0
30	0.0	81.925	0.0	0.0	0.0
31	0.0	82.625	0.0	0.0	0.0
32	0.0	83.65	0.0	0.0	0.0
33	0.0	84.875	0.0	0.0	0.0
34	0.0	87.125	0.0	0.0	0.0
35	0.0	88.3	0.0	0.0	0.0
36	0.0	89.05	0.0	0.0	0.0
37	0.0	90.025	0.0	0.0	0.0
38	0.0	90.425	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
TGGTTCA	20	6.952051E-4	44.000004	8
AAGTAAT	65	0.0	44.000004	4
TCAAGTA	65	0.0	44.000004	2
CATTGGT	20	6.952051E-4	44.000004	2
CTGAGAC	20	6.952051E-4	44.000004	4
TAAACAT	20	6.952051E-4	44.000004	3
AAACATC	20	6.952051E-4	44.000004	4
GAGTGGA	20	6.952051E-4	44.000004	20
TGGTAGA	20	6.952051E-4	44.000004	16
TTCAAGT	65	0.0	44.000004	1
AGGATAG	65	0.0	44.000004	13
ATTGGTG	20	6.952051E-4	44.000004	3
AGTGGTA	20	6.952051E-4	44.000004	14
AGTGGAA	20	6.952051E-4	44.000004	21
CCTGAGA	20	6.952051E-4	44.000004	3
GGTTCAG	20	6.952051E-4	44.000004	9
GTTCAGT	20	6.952051E-4	44.000004	10
AGAATTC	20	6.952051E-4	44.000004	20
GGATAGG	65	0.0	44.000004	14
>>END_MODULE
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359400 spots for SRR5031058.sra
Written 359400 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
Read 359392 spots for SRR5031058.sra
Written 359392 spots for SRR5031058.sra
SRR ids: ['SRR5031058.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_boimcrbe
SRR5031058.sra spots: 4312712
blocks: [[1, 359392], [359393, 718784], [718785, 1078176], [1078177, 1437568], [1437569, 1796960], [1796961, 2156352], [2156353, 2515744], [2515745, 2875136], [2875137, 3234528], [3234529, 3593920], [3593921, 3953312], [3953313, 4312712]]
SRR5031058 file size 746431
SRR5031058 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031058 SRR5031058_1.fastq
Input file:	SRR5031058_1.fastq
trimmed:	SRR5031058-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:15:30 2020 >> started

Wed Mar  4 07:15:33 2020 >> done (2.631s)
4312712 reads processed; of these:
    254 ( 0.01%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
4312450 (99.99%) reads available; of these:
 220188 ( 5.11%) trimmed reads available after processing
4092262 (94.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     73	  0.00%
 19	    124	  0.00%
 20	    222	  0.01%
 21	    323	  0.01%
 22	    570	  0.01%
 23	    604	  0.01%
 24	    600	  0.01%
 25	    864	  0.02%
 26	    581	  0.01%
 27	    858	  0.02%
 28	   1089	  0.03%
 29	   2037	  0.05%
 30	   1044	  0.02%
 31	   1157	  0.03%
 32	   1161	  0.03%
 33	   1445	  0.03%
 34	   1965	  0.05%
 35	   3864	  0.09%
 36	   3236	  0.08%
 37	   3416	  0.08%
 38	   3321	  0.08%
 39	   3779	  0.09%
 40	   4422	  0.10%
 41	   4615	  0.11%
 42	   5940	  0.14%
 43	   8603	  0.20%
 44	  12189	  0.28%
 45	  15836	  0.37%
 46	  20315	  0.47%
 47	  27150	  0.63%
 48	  45033	  1.04%
 49	  43752	  1.01%
 50	4092262	 94.89%
4312450 reads passed initial QC


criterion=sequence-density
sequence-density=90.15
sequence-density-rank=1
fanout-score=18.62
fanout-score-rank=1
prefix-density=92.06
prefix-fanout=18.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC


criterion=fanout-score
sequence-density=90.15
sequence-density-rank=1
fanout-score=18.62
fanout-score-rank=1
prefix-density=92.06
prefix-fanout=18.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC -o SRR5031058 -
Input file:	STDIN
trimmed:	SRR5031058-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:15:53 2020 >> started

Wed Mar  4 07:15:58 2020 >> done (5.468s)
4217671 reads processed; of these:
 165385 ( 3.92%) short reads filtered out after trimming by size control
  12018 ( 0.28%) empty reads filtered out after trimming by size control
4040268 (95.79%) reads available; of these:
3918699 (96.99%) trimmed reads available after processing
 121569 ( 3.01%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  22239	  0.55%
 19	  46647	  1.15%
 20	 193812	  4.80%
 21	 484332	 11.99%
 22	1495987	 37.03%
 23	 702322	 17.38%
 24	 246151	  6.09%
 25	  45021	  1.11%
 26	  22933	  0.57%
 27	  23933	  0.59%
 28	  27526	  0.68%
 29	  21264	  0.53%
 30	  36461	  0.90%
 31	  47107	  1.17%
 32	  54723	  1.35%
 33	  92920	  2.30%
 34	  48624	  1.20%
 35	  31457	  0.78%
 36	  37133	  0.92%
 37	  19355	  0.48%
 38	  22282	  0.55%
 39	  19871	  0.49%
 40	  26460	  0.65%
 41	  34181	  0.85%
 42	  34873	  0.86%
 43	  25483	  0.63%
 44	  20506	  0.51%
 45	  22653	  0.56%
 46	  16940	  0.42%
 47	  12987	  0.32%
 48	   1318	  0.03%
 49	   2192	  0.05%
 50	 100575	  2.49%


criterion=sequence-density
sequence-density=3.59
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=12
prefix-density=0.01
prefix-fanout=1.0
sequence=TGTAAACATCCT


criterion=fanout-score
sequence-density=0.40
sequence-density-rank=29
fanout-score=15.02
fanout-score-rank=1
prefix-density=6.05
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 07:16:31
                             Started mapping on |	Mar 04 07:16:32
                                    Finished on |	Mar 04 07:16:57
       Mapping speed, Million of reads per hour |	595.45

                          Number of input reads |	4135047
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2003204
                        Uniquely mapped reads % |	48.44%
                          Average mapped length |	24.19
                       Number of splices: Total |	2333
            Number of splices: Annotated (sjdb) |	469
                       Number of splices: GT/AG |	2158
                       Number of splices: GC/AG |	24
                       Number of splices: AT/AC |	5
               Number of splices: Non-canonical |	146
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.71
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1741066
             % of reads mapped to multiple loci |	42.11%
        Number of reads mapped to too many loci |	97769
             % of reads mapped to too many loci |	2.36%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.38%
                     % of reads unmapped: other |	0.70%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	390777	390777	390777
N_multimapping	1741066	1741066	1741066
N_noFeature	88319	108763	1543106
N_ambiguous	1075037	691520	13807
UnstrandedReadsAssigned:839848 PositiveStrandReadsAssigned:1202921 NegativeStrandReadsAssigned:446291
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031058 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031058-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,135,047 reads, 870,894 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 834 rounds

 109283 SRR5031058.ke.tsv
  52636 SRR5031058.se.tsv
 161919 total
==> SRR5031058.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031058.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031058 completed mapping pipeline successfully
