Starting /dee2/code/volunteer_pipeline.sh SRR5031108
    current disk space = 160468713472
    free memory = 73965484 
SRR5031108 SRAfilesize
bbffd2bcf76b89fe808171fe6144ae4c  SRR5031108.sra
SRR5031108.sra file validated
SRR5031108 is single end
SRR5031108 is conventional basespace
SRR5031108 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9725	31.0	31.0	34.0	30.0	34.0
2	32.4455	33.0	31.0	34.0	31.0	34.0
3	32.42775	34.0	31.0	34.0	31.0	34.0
4	36.1765	37.0	35.0	37.0	35.0	37.0
5	36.1115	37.0	35.0	37.0	35.0	37.0
6	36.05975	37.0	35.0	37.0	35.0	37.0
7	35.833	37.0	35.0	37.0	35.0	37.0
8	35.6865	37.0	35.0	37.0	33.0	37.0
9	36.8855	39.0	37.0	39.0	33.0	39.0
10	36.6305	39.0	35.0	39.0	32.0	39.0
11	37.437	39.0	37.0	39.0	34.0	39.0
12	37.72225	39.0	37.0	39.0	35.0	39.0
13	37.51625	39.0	37.0	39.0	35.0	39.0
14	38.33825	40.0	38.0	41.0	33.0	41.0
15	36.716	39.0	36.0	41.0	29.0	41.0
16	36.67275	38.0	36.0	40.0	31.0	41.0
17	36.5285	39.0	36.0	40.0	30.0	41.0
18	38.1925	39.0	37.0	41.0	34.0	41.0
19	38.64625	40.0	38.0	41.0	35.0	41.0
20	38.46925	40.0	38.0	41.0	34.0	41.0
21	38.58275	40.0	38.0	41.0	35.0	41.0
22	37.39225	39.0	37.0	40.0	32.0	41.0
23	37.93725	39.0	37.0	40.0	34.0	41.0
24	37.302	39.0	37.0	40.0	32.0	41.0
25	38.13175	40.0	38.0	41.0	34.0	41.0
26	37.898	40.0	38.0	41.0	33.0	41.0
27	38.1545	40.0	38.0	41.0	34.0	41.0
28	38.38675	40.0	38.0	41.0	34.0	41.0
29	38.37875	40.0	38.0	41.0	34.0	41.0
30	38.7495	40.0	38.0	41.0	35.0	41.0
31	38.71	40.0	38.0	41.0	35.0	41.0
32	38.78725	40.0	38.0	41.0	35.0	41.0
33	38.835	40.0	38.0	41.0	35.0	41.0
34	38.23075	40.0	38.0	41.0	33.0	41.0
35	37.41325	40.0	37.0	41.0	30.0	41.0
36	32.8435	35.0	30.0	40.0	23.0	41.0
37	34.8455	37.0	33.0	39.0	30.0	40.0
38	36.33925	38.0	35.0	39.0	31.0	40.0
39	36.67175	38.0	36.0	40.0	31.0	40.0
40	37.01425	39.0	37.0	40.0	32.0	41.0
41	37.51025	39.0	37.0	40.0	33.0	41.0
42	37.45675	40.0	37.0	41.0	32.0	41.0
43	37.463	40.0	37.0	41.0	32.0	41.0
44	36.55325	39.0	35.0	40.0	30.0	41.0
45	36.72375	39.0	36.0	40.0	31.0	41.0
46	36.90475	39.0	36.0	40.0	31.0	41.0
47	36.006	38.0	35.0	40.0	28.0	41.0
48	37.11475	39.0	37.0	40.0	31.0	41.0
49	37.21225	40.0	37.0	41.0	32.0	41.0
50	35.2115	38.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	1.0
16	1.0
17	0.0
18	2.0
19	3.0
20	4.0
21	5.0
22	6.0
23	7.0
24	3.0
25	6.0
26	14.0
27	15.0
28	17.0
29	35.0
30	49.0
31	89.0
32	77.0
33	149.0
34	192.0
35	267.0
36	449.0
37	796.0
38	1144.0
39	667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.903951975987994	28.83941970985493	49.92496248124062	13.331665832916459
2	22.436218109054526	32.641320660330166	16.983491745872936	27.938969484742373
3	25.1	16.85	18.325	39.725
4	34.1	31.95	11.675	22.275
5	21.85	36.1	30.425	11.625
6	28.199999999999996	13.975000000000001	43.175000000000004	14.649999999999999
7	14.274999999999999	21.175	20.125	44.425
8	20.925	32.425	15.6	31.05
9	24.099999999999998	36.275	25.900000000000002	13.725000000000001
10	19.975	16.425	30.099999999999998	33.5
11	36.325	10.8	14.825	38.05
12	13.475000000000001	19.325	32.6	34.599999999999994
13	27.3	14.549999999999999	37.275000000000006	20.875
14	32.300000000000004	28.799999999999997	22.175	16.725
15	36.825	16.075	26.775	20.325
16	19.375	16.575	21.525	42.525
17	28.975	21.8	34.325	14.899999999999999
18	28.825	14.524999999999999	32.1	24.55
19	34.825	13.275	35.15	16.75
20	50.625	11.3	25.35	12.725
21	24.45	23.0	24.25	28.299999999999997
22	31.874999999999996	10.299999999999999	47.875	9.950000000000001
23	25.75	11.725	50.9	11.625
24	52.625	11.575000000000001	27.750000000000004	8.05
25	54.6	24.224999999999998	15.049999999999999	6.125
26	28.199999999999996	49.5	13.05	9.25
27	14.924999999999999	51.65	23.7	9.725
28	9.35	26.424999999999997	50.475	13.750000000000002
29	10.625	10.4	60.175	18.8
30	8.1	4.6	38.9	48.4
31	12.575	3.4250000000000003	49.175000000000004	34.825
32	26.825	4.35	23.200000000000003	45.625
33	57.775	6.45	12.174999999999999	23.599999999999998
34	67.375	4.9	13.725000000000001	14.000000000000002
35	69.6	7.049999999999999	16.475	6.875000000000001
36	45.175	6.375	36.5	11.95
37	47.825	7.8	21.375	23.0
38	24.0	13.700000000000001	11.25	51.05
39	13.25	24.65	7.35	54.75
40	13.200000000000001	51.225	7.625	27.950000000000003
41	23.375	53.675	6.4750000000000005	16.475
42	51.65	32.275	7.825	8.25
43	55.275	29.675	5.975	9.075
44	30.525000000000002	50.575	4.725	14.174999999999999
45	16.25	54.7	11.325000000000001	17.724999999999998
46	10.274999999999999	27.725	16.8	45.2
47	6.775	13.5	39.725	40.0
48	7.6	11.85	20.575	59.975
49	11.025	19.275000000000002	11.4	58.3
50	16.35	40.025	11.075	32.550000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	1.5
30	2.0
31	1.5
32	1.0
33	4.0
34	7.0
35	6.5
36	6.0
37	9.5
38	13.0
39	15.5
40	18.0
41	23.5
42	29.0
43	79.5
44	130.0
45	123.0
46	116.0
47	238.5
48	361.0
49	483.0
50	605.0
51	821.0
52	1037.0
53	734.0
54	431.0
55	495.0
56	559.0
57	433.0
58	307.0
59	219.5
60	132.0
61	87.5
62	43.0
63	75.0
64	107.0
65	76.5
66	46.0
67	31.0
68	16.0
69	13.5
70	11.0
71	14.5
72	18.0
73	9.5
74	1.0
75	1.5
76	2.0
77	1.0
78	0.0
79	0.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26708074534162	31.1
2	8.944099378881988	7.199999999999999
3	3.7267080745341614	4.5
4	2.360248447204969	3.8
5	1.5527950310559007	3.125
6	1.0559006211180124	2.55
7	0.9316770186335404	2.625
8	0.43478260869565216	1.4000000000000001
9	0.37267080745341613	1.35
>10	2.7950310559006213	18.675
>50	0.37267080745341613	11.5
>100	0.18633540372670807	12.174999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	194	4.8500000000000005	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	147	3.675	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	146	3.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	90	2.25	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	84	2.1	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	79	1.975	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	78	1.95	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	55	1.375	No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	49	1.225	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	42	1.05	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	21	0.525	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC	17	0.42500000000000004	RNA PCR Primer, Index 32 (100% over 50bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	11	0.27499999999999997	RNA PCR Primer, Index 7 (100% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC	11	0.27499999999999997	RNA PCR Primer, Index 32 (100% over 44bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (96% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	9	0.22499999999999998	RNA PCR Primer, Index 2 (100% over 34bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	7	0.17500000000000002	No Hit
TGAGGTAGTCGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCTGGAATTCTCGGGTG	6	0.15	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGACGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACCCTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACCATTCGTAGACGCCCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	5	0.125	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGGGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCATTGCTGTCGGTGGGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCCGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCCCTGAGACCCTTTACCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGGCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
CGCGACCTCCGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	5	0.125	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.625	0.0	0.0	0.0
2	0.0	0.65	0.0	0.0	0.0
3	0.0	0.775	0.0	0.0	0.0
4	0.0	0.8	0.0	0.0	0.0
5	0.0	0.85	0.0	0.0	0.0
6	0.0	0.95	0.0	0.0	0.0
7	0.0	1.4	0.0	0.0	0.0
8	0.0	1.625	0.0	0.0	0.0
9	0.0	1.65	0.0	0.0	0.0
10	0.0	1.775	0.0	0.0	0.0
11	0.0	1.875	0.0	0.0	0.0
12	0.0	1.925	0.0	0.0	0.0
13	0.0	2.15	0.0	0.0	0.0
14	0.0	2.35	0.0	0.0	0.0
15	0.0	2.75	0.0	0.0	0.0
16	0.0	3.525	0.0	0.0	0.0
17	0.0	4.275	0.0	0.0	0.0
18	0.0	5.3	0.0	0.0	0.0
19	0.0	5.9	0.0	0.0	0.0
20	0.0	6.7	0.0	0.0	0.0
21	0.0	12.25	0.0	0.0	0.0
22	0.0	24.375	0.0	0.0	0.0
23	0.0	57.75	0.0	0.0	0.0
24	0.0	73.55	0.0	0.0	0.0
25	0.0	79.9	0.0	0.0	0.0
26	0.0	81.225	0.0	0.0	0.0
27	0.0	81.75	0.0	0.0	0.0
28	0.0	82.6	0.0	0.0	0.0
29	0.0	83.2	0.0	0.0	0.0
30	0.0	83.525	0.0	0.0	0.0
31	0.0	84.275	0.0	0.0	0.0
32	0.0	85.65	0.0	0.0	0.0
33	0.0	86.4	0.0	0.0	0.0
34	0.0	88.825	0.0	0.0	0.0
35	0.0	89.775	0.0	0.0	0.0
36	0.0	90.525	0.0	0.0	0.0
37	0.0	91.375	0.0	0.0	0.0
38	0.0	91.55	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTCAA	80	0.0	44.000004	3
GAGTTGG	40	5.315087E-9	44.000004	20
GCTGTCG	80	0.0	44.000004	11
TGAGTTG	40	5.315087E-9	44.000004	19
ATTCAAC	80	0.0	44.000004	4
AACGCTG	75	0.0	44.0	8
ACGCTGT	75	0.0	44.0	9
TTCAACG	75	0.0	44.0	5
TCAACGC	75	0.0	44.0	6
GTGAGTG	30	1.9515464E-6	44.0	18
ACATTCA	85	0.0	44.0	2
GGCTTGG	25	3.6958358E-5	44.0	19
CTGTTGG	25	3.6958358E-5	44.0	19
AACATTC	85	0.0	44.0	1
CAACGCT	75	0.0	44.0	7
CGCTGTC	75	0.0	44.0	10
CTGTCGG	85	0.0	41.411766	12
GTCGGTG	85	0.0	41.411766	14
TGTCGGT	85	0.0	41.411766	13
TCGGTGA	80	0.0	41.250004	15
>>END_MODULE
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77002 spots for SRR5031108.sra
Written 77002 spots for SRR5031108.sra
Read 77004 spots for SRR5031108.sra
Written 77004 spots for SRR5031108.sra
SRR ids: ['SRR5031108.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_rtp2t2sy
SRR5031108.sra spots: 924026
blocks: [[1, 77002], [77003, 154004], [154005, 231006], [231007, 308008], [308009, 385010], [385011, 462012], [462013, 539014], [539015, 616016], [616017, 693018], [693019, 770020], [770021, 847022], [847023, 924026]]
SRR5031108 file size 159151
SRR5031108 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031108 SRR5031108_1.fastq
Input file:	SRR5031108_1.fastq
trimmed:	SRR5031108-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:25:35 2020 >> started

Wed Mar  4 07:25:37 2020 >> done (1.640s)
924026 reads processed; of these:
   101 ( 0.01%) short reads filtered out after trimming by size control
     2 ( 0.00%) empty reads filtered out after trimming by size control
923923 (99.99%) reads available; of these:
 76329 ( 8.26%) trimmed reads available after processing
847594 (91.74%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    27	  0.00%
 19	    60	  0.01%
 20	    72	  0.01%
 21	   110	  0.01%
 22	   165	  0.02%
 23	   207	  0.02%
 24	   200	  0.02%
 25	   282	  0.03%
 26	   210	  0.02%
 27	   255	  0.03%
 28	   262	  0.03%
 29	   469	  0.05%
 30	   259	  0.03%
 31	   339	  0.04%
 32	   410	  0.04%
 33	   748	  0.08%
 34	  1063	  0.12%
 35	  2605	  0.28%
 36	  1457	  0.16%
 37	  1411	  0.15%
 38	  1410	  0.15%
 39	  1420	  0.15%
 40	  1697	  0.18%
 41	  1859	  0.20%
 42	  2364	  0.26%
 43	  3903	  0.42%
 44	  3989	  0.43%
 45	  5042	  0.55%
 46	  6593	  0.71%
 47	  5708	  0.62%
 48	 16059	  1.74%
 49	 15674	  1.70%
 50	847594	 91.74%
923923 reads passed initial QC


criterion=sequence-density
sequence-density=90.83
sequence-density-rank=1
fanout-score=20.62
fanout-score-rank=2
prefix-density=91.95
prefix-fanout=20.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC


criterion=fanout-score
sequence-density=3.80
sequence-density-rank=2
fanout-score=73.69
fanout-score-rank=1
prefix-density=92.12
prefix-fanout=3.0
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC -o SRR5031108 -
Input file:	STDIN
trimmed:	SRR5031108-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:25:49 2020 >> started

Wed Mar  4 07:25:51 2020 >> done (2.353s)
903617 reads processed; of these:
 50856 ( 5.63%) short reads filtered out after trimming by size control
  6124 ( 0.68%) empty reads filtered out after trimming by size control
846637 (93.69%) reads available; of these:
823918 (97.32%) trimmed reads available after processing
 22719 ( 2.68%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  5949	  0.70%
 19	  9587	  1.13%
 20	 50967	  6.02%
 21	111822	 13.21%
 22	302615	 35.74%
 23	140735	 16.62%
 24	 57091	  6.74%
 25	 15129	  1.79%
 26	  7598	  0.90%
 27	  5938	  0.70%
 28	  6036	  0.71%
 29	  5198	  0.61%
 30	  7027	  0.83%
 31	 13745	  1.62%
 32	  8125	  0.96%
 33	 26047	  3.08%
 34	  8364	  0.99%
 35	  6465	  0.76%
 36	  7513	  0.89%
 37	  3001	  0.35%
 38	  3359	  0.40%
 39	  2615	  0.31%
 40	  4164	  0.49%
 41	  4087	  0.48%
 42	  4358	  0.51%
 43	  2984	  0.35%
 44	  2131	  0.25%
 45	  2626	  0.31%
 46	  1789	  0.21%
 47	  1530	  0.18%
 48	   248	  0.03%
 49	   378	  0.04%
 50	 17416	  2.06%


criterion=sequence-density
sequence-density=11.77
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=11
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTGGAATTCT


criterion=fanout-score
sequence-density=2.21
sequence-density-rank=7
fanout-score=17.22
fanout-score-rank=1
prefix-density=2.11
prefix-fanout=17.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC
                                 Started job on |	Mar 04 07:26:19
                             Started mapping on |	Mar 04 07:26:20
                                    Finished on |	Mar 04 07:26:45
       Mapping speed, Million of reads per hour |	124.84

                          Number of input reads |	866943
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	458789
                        Uniquely mapped reads % |	52.92%
                          Average mapped length |	23.38
                       Number of splices: Total |	1718
            Number of splices: Annotated (sjdb) |	480
                       Number of splices: GT/AG |	1692
                       Number of splices: GC/AG |	10
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	14
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.75
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	322554
             % of reads mapped to multiple loci |	37.21%
        Number of reads mapped to too many loci |	12795
             % of reads mapped to too many loci |	1.48%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.75%
                     % of reads unmapped: other |	0.65%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	85600	85600	85600
N_multimapping	322554	322554	322554
N_noFeature	31148	35142	295814
N_ambiguous	259261	111624	2803
UnstrandedReadsAssigned:168380 PositiveStrandReadsAssigned:312023 NegativeStrandReadsAssigned:160172
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031108 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031108-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 866,943 reads, 222,944 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,018 rounds

 109283 SRR5031108.ke.tsv
  52636 SRR5031108.se.tsv
 161919 total
==> SRR5031108.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031108.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031108 completed mapping pipeline successfully
