Starting /dee2/code/volunteer_pipeline.sh SRR5031019
    current disk space = 160550076416
    free memory = 73950052 
SRR5031019 SRAfilesize
87308086c5122a4cf0a3d9186fa1a022  SRR5031019.sra
SRR5031019.sra file validated
SRR5031019 is single end
SRR5031019 is conventional basespace
SRR5031019 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2795	33.0	31.0	34.0	30.0	34.0
2	32.52925	34.0	31.0	34.0	31.0	34.0
3	32.60775	34.0	31.0	34.0	31.0	34.0
4	36.199	37.0	35.0	37.0	35.0	37.0
5	36.0245	37.0	35.0	37.0	35.0	37.0
6	35.84575	37.0	35.0	37.0	33.0	37.0
7	35.8025	37.0	35.0	37.0	35.0	37.0
8	35.82775	37.0	35.0	37.0	35.0	37.0
9	37.486	39.0	37.0	39.0	35.0	39.0
10	37.53225	39.0	37.0	39.0	35.0	39.0
11	37.06525	39.0	37.0	39.0	33.0	39.0
12	37.56425	39.0	37.0	39.0	35.0	39.0
13	37.52725	39.0	37.0	39.0	35.0	39.0
14	38.93275	40.0	38.0	41.0	35.0	41.0
15	38.903	40.0	38.0	41.0	35.0	41.0
16	38.81525	40.0	38.0	41.0	35.0	41.0
17	38.98725	40.0	38.0	41.0	35.0	41.0
18	38.8675	40.0	38.0	41.0	35.0	41.0
19	38.75575	40.0	38.0	41.0	34.0	41.0
20	38.72975	40.0	38.0	41.0	34.0	41.0
21	38.7075	40.0	38.0	41.0	34.0	41.0
22	38.7735	40.0	38.0	41.0	35.0	41.0
23	38.4355	40.0	38.0	41.0	34.0	41.0
24	38.685	40.0	38.0	41.0	34.0	41.0
25	38.858	40.0	38.0	41.0	35.0	41.0
26	38.78275	40.0	38.0	41.0	35.0	41.0
27	38.86725	40.0	38.0	41.0	35.0	41.0
28	38.54325	40.0	38.0	41.0	34.0	41.0
29	38.1705	40.0	38.0	41.0	33.0	41.0
30	38.0195	40.0	38.0	41.0	32.0	41.0
31	38.29525	40.0	38.0	41.0	33.0	41.0
32	38.258	40.0	38.0	41.0	33.0	41.0
33	37.99875	40.0	38.0	41.0	33.0	41.0
34	38.1635	40.0	38.0	41.0	34.0	41.0
35	38.04	40.0	38.0	41.0	33.0	41.0
36	37.71275	40.0	37.0	41.0	33.0	41.0
37	37.27925	39.0	36.0	41.0	31.0	41.0
38	36.923	39.0	36.0	40.0	30.0	41.0
39	37.434	39.0	37.0	41.0	32.0	41.0
40	37.56475	40.0	37.0	41.0	32.0	41.0
41	37.7555	40.0	37.0	41.0	33.0	41.0
42	37.61275	40.0	37.0	41.0	33.0	41.0
43	37.70625	40.0	37.0	41.0	33.0	41.0
44	37.58425	40.0	37.0	41.0	33.0	41.0
45	37.49425	40.0	37.0	41.0	33.0	41.0
46	37.17575	40.0	36.0	41.0	31.0	41.0
47	36.584	40.0	35.0	41.0	30.0	41.0
48	36.3285	39.0	35.0	41.0	30.0	41.0
49	36.43525	39.0	35.0	41.0	30.0	41.0
50	35.83425	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	2.0
20	3.0
21	4.0
22	8.0
23	9.0
24	7.0
25	8.0
26	18.0
27	26.0
28	30.0
29	34.0
30	52.0
31	58.0
32	97.0
33	115.0
34	183.0
35	229.0
36	271.0
37	435.0
38	743.0
39	1665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.774999999999999	24.6	45.574999999999996	18.05
2	24.956195244055067	24.98122653316646	18.94868585732165	31.11389236545682
3	25.75	18.675	22.925	32.65
4	36.075	24.775	15.950000000000001	23.200000000000003
5	22.925	37.7	27.474999999999998	11.899999999999999
6	34.375	13.725000000000001	33.900000000000006	18.0
7	19.125	20.849999999999998	21.325	38.7
8	24.224999999999998	26.125	17.675	31.974999999999998
9	23.974999999999998	30.575000000000003	26.875	18.575
10	22.525000000000002	20.974999999999998	28.549999999999997	27.950000000000003
11	31.225	11.175	18.55	39.050000000000004
12	17.625	18.025	31.324999999999996	33.025
13	31.8	17.575	32.675	17.95
14	29.7	31.95	20.150000000000002	18.2
15	37.5	22.925	24.7	14.875
16	20.875	16.825000000000003	25.174999999999997	37.125
17	27.650000000000002	21.675	36.1	14.575
18	25.6	17.525	28.725	28.15
19	36.375	14.025000000000002	30.8	18.8
20	43.225	15.725	28.175	12.875
21	24.875	21.175	25.05	28.9
22	29.275000000000002	13.875000000000002	45.0	11.85
23	27.375	14.174999999999999	41.675000000000004	16.775000000000002
24	48.175000000000004	15.0	26.325	10.5
25	48.1	22.650000000000002	16.875	12.375
26	29.5	42.0	15.2	13.3
27	17.4	43.675000000000004	22.775000000000002	16.150000000000002
28	14.799999999999999	23.45	44.800000000000004	16.950000000000003
29	15.325	12.25	52.65	19.775000000000002
30	12.575	7.375	34.2	45.85
31	16.1	5.875	43.575	34.449999999999996
32	28.525	6.0	22.85	42.625
33	50.05	10.075000000000001	15.6	24.275
34	56.525000000000006	8.450000000000001	17.299999999999997	17.724999999999998
35	60.724999999999994	8.75	19.55	10.975
36	40.925	9.1	35.25	14.725
37	42.8	12.65	22.375	22.175
38	25.674999999999997	17.9	13.65	42.775
39	17.8	22.7	11.75	47.75
40	16.35	44.975	14.249999999999998	24.425
41	24.175	45.375	11.450000000000001	19.0
42	44.55	27.85	15.6	12.0
43	49.4	26.724999999999998	10.5	13.375
44	32.824999999999996	43.475	9.375	14.325
45	19.0	46.325	15.950000000000001	18.725
46	16.950000000000003	24.75	17.825	40.475
47	12.35	15.6	37.4	34.65
48	13.575000000000001	12.9	21.75	51.775000000000006
49	13.600000000000001	19.025	15.9	51.475
50	18.05	34.875	16.0	31.075000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	2.5
30	3.0
31	11.5
32	20.0
33	14.5
34	9.0
35	12.0
36	15.0
37	23.5
38	32.0
39	34.0
40	36.0
41	42.5
42	49.0
43	83.0
44	117.0
45	134.5
46	152.0
47	271.0
48	390.0
49	499.0
50	608.0
51	694.0
52	780.0
53	601.5
54	423.0
55	503.5
56	584.0
57	418.5
58	253.0
59	167.5
60	82.0
61	66.5
62	51.0
63	112.0
64	173.0
65	105.0
66	37.0
67	32.5
68	28.0
69	35.0
70	42.0
71	73.5
72	105.0
73	55.5
74	6.0
75	3.5
76	1.0
77	0.5
78	0.0
79	0.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.65
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56042496679947	28.825
2	8.632138114209829	6.5
3	4.183266932270916	4.725
4	2.058432934926959	3.1
5	1.7928286852589643	3.375
6	0.7304116865869853	1.6500000000000001
7	1.1288180610889775	2.9749999999999996
8	0.5976095617529881	1.7999999999999998
9	0.5312084993359893	1.7999999999999998
>10	3.187250996015936	24.05
>50	0.33200531208499334	8.799999999999999
>100	0.2656042496679947	12.4
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	136	3.4000000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	133	3.325	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	115	2.875	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	112	2.8000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	100	2.5	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	85	2.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	53	1.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	50	1.25	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	48	1.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	48	1.2	RNA PCR Primer, Index 1 (97% over 35bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	32	0.8	RNA PCR Primer, Index 1 (100% over 33bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	32	0.8	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	26	0.65	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	19	0.475	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	19	0.475	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	18	0.44999999999999996	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTC	13	0.325	RNA PCR Primer, Index 39 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	13	0.325	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	12	0.3	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	12	0.3	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	12	0.3	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGC	11	0.27499999999999997	RNA PCR Primer, Index 39 (100% over 50bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	11	0.27499999999999997	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	10	0.25	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	10	0.25	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	8	0.2	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	8	0.2	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 24bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AGAAATGCGGATATGGGACTGAGATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTAT	7	0.17500000000000002	RNA PCR Primer, Index 39 (100% over 48bp)
AAGGGATTCTGATGTTGGTCACACTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	6	0.15	No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	5	0.125	RNA PCR Primer, Index 1 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGTG	5	0.125	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.425	0.0	0.0	0.0
3	0.0	0.675	0.0	0.0	0.0
4	0.0	0.675	0.0	0.0	0.0
5	0.0	0.725	0.0	0.0	0.0
6	0.0	0.9	0.0	0.0	0.0
7	0.0	1.3	0.0	0.0	0.0
8	0.0	1.475	0.0	0.0	0.0
9	0.0	1.525	0.0	0.0	0.0
10	0.0	1.75	0.0	0.0	0.0
11	0.0	1.875	0.0	0.0	0.0
12	0.0	2.05	0.0	0.0	0.0
13	0.0	2.35	0.0	0.0	0.0
14	0.0	2.6	0.0	0.0	0.0
15	0.0	2.9	0.0	0.0	0.0
16	0.0	4.6	0.0	0.0	0.0
17	0.0	5.35	0.0	0.0	0.0
18	0.0	6.675	0.0	0.0	0.0
19	0.0	7.1	0.0	0.0	0.0
20	0.0	8.175	0.0	0.0	0.0
21	0.0	13.05	0.0	0.0	0.0
22	0.0	22.55	0.0	0.0	0.0
23	0.0	48.45	0.0	0.0	0.0
24	0.0	60.55	0.0	0.0	0.0
25	0.0	66.175	0.0	0.0	0.0
26	0.0	67.5	0.0	0.0	0.0
27	0.0	68.475	0.0	0.0	0.0
28	0.0	69.1	0.0	0.0	0.0
29	0.0	69.925	0.0	0.0	0.0
30	0.0	70.575	0.0	0.0	0.0
31	0.0	71.425	0.0	0.0	0.0
32	0.0	73.25	0.0	0.0	0.0
33	0.0	74.625	0.0	0.0	0.0
34	0.0	78.35	0.0	0.0	0.0
35	0.0	79.7	0.0	0.0	0.0
36	0.0	81.375	0.0	0.0	0.0
37	0.0	83.25	0.0	0.0	0.0
38	0.0	83.8	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGGA	20	6.952051E-4	44.000004	20
GGTGAGT	20	6.952051E-4	44.000004	17
CTGAGCT	40	5.315087E-9	44.000004	11
GAGCTTG	40	5.315087E-9	44.000004	13
TTGGCTT	20	6.952051E-4	44.000004	17
TGAGCTT	40	5.315087E-9	44.000004	12
CGTCTGA	40	5.315087E-9	44.000004	8
AACGCTG	25	3.6958358E-5	44.0	8
AAGTAAT	25	3.6958358E-5	44.0	4
TCAAGTA	25	3.6958358E-5	44.0	2
ACGCTGT	25	3.6958358E-5	44.0	9
AATCCAG	25	3.6958358E-5	44.0	8
ATCCAGG	25	3.6958358E-5	44.0	9
TTCAAGT	25	3.6958358E-5	44.0	1
TTCAACG	25	3.6958358E-5	44.0	5
AGGATAG	25	3.6958358E-5	44.0	13
TCGGTGA	25	3.6958358E-5	44.0	15
ATAGGCT	25	3.6958358E-5	44.0	16
GGATCCG	35	1.02281774E-7	44.0	3
CCGTCTG	35	1.02281774E-7	44.0	7
>>END_MODULE
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
Read 138209 spots for SRR5031019.sra
Written 138209 spots for SRR5031019.sra
Read 138205 spots for SRR5031019.sra
Written 138205 spots for SRR5031019.sra
SRR ids: ['SRR5031019.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bq0h3qif
SRR5031019.sra spots: 1658464
blocks: [[1, 138205], [138206, 276410], [276411, 414615], [414616, 552820], [552821, 691025], [691026, 829230], [829231, 967435], [967436, 1105640], [1105641, 1243845], [1243846, 1382050], [1382051, 1520255], [1520256, 1658464]]
SRR5031019 file size 286374
SRR5031019 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031019 SRR5031019_1.fastq
Input file:	SRR5031019_1.fastq
trimmed:	SRR5031019-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:07:12 2020 >> started

Wed Mar  4 07:07:13 2020 >> done (1.188s)
1658464 reads processed; of these:
    195 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
1658265 (99.99%) reads available; of these:
 103035 ( 6.21%) trimmed reads available after processing
1555230 (93.79%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     54	  0.00%
 19	     58	  0.00%
 20	    108	  0.01%
 21	    146	  0.01%
 22	    247	  0.01%
 23	    283	  0.02%
 24	    292	  0.02%
 25	    406	  0.02%
 26	    324	  0.02%
 27	    371	  0.02%
 28	    438	  0.03%
 29	    687	  0.04%
 30	    455	  0.03%
 31	    510	  0.03%
 32	    591	  0.04%
 33	    709	  0.04%
 34	    885	  0.05%
 35	   1543	  0.09%
 36	   1376	  0.08%
 37	   1630	  0.10%
 38	   1677	  0.10%
 39	   1795	  0.11%
 40	   1949	  0.12%
 41	   2152	  0.13%
 42	   2847	  0.17%
 43	   4081	  0.25%
 44	   5341	  0.32%
 45	   6657	  0.40%
 46	  10306	  0.62%
 47	  11957	  0.72%
 48	  18802	  1.13%
 49	  24358	  1.47%
 50	1555230	 93.79%
1658265 reads passed initial QC


criterion=sequence-density
sequence-density=84.09
sequence-density-rank=1
fanout-score=21.68
fanout-score-rank=1
prefix-density=86.46
prefix-fanout=21.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC


criterion=fanout-score
sequence-density=84.09
sequence-density-rank=1
fanout-score=21.68
fanout-score-rank=1
prefix-density=86.46
prefix-fanout=21.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC -o SRR5031019 -
Input file:	STDIN
trimmed:	SRR5031019-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:07:26 2020 >> started

Wed Mar  4 07:07:28 2020 >> done (1.679s)
1619247 reads processed; of these:
 115249 ( 7.12%) short reads filtered out after trimming by size control
   7227 ( 0.45%) empty reads filtered out after trimming by size control
1496771 (92.44%) reads available; of these:
1409093 (94.14%) trimmed reads available after processing
  87678 ( 5.86%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   8498	  0.57%
 19	  17473	  1.17%
 20	  74660	  4.99%
 21	 160250	 10.71%
 22	 422463	 28.22%
 23	 199500	 13.33%
 24	  89040	  5.95%
 25	  25568	  1.71%
 26	  13994	  0.93%
 27	  11314	  0.76%
 28	  12523	  0.84%
 29	  10262	  0.69%
 30	  15499	  1.04%
 31	  27774	  1.86%
 32	  20014	  1.34%
 33	  72278	  4.83%
 34	  27472	  1.84%
 35	  23961	  1.60%
 36	  32443	  2.17%
 37	  10708	  0.72%
 38	  12072	  0.81%
 39	  10038	  0.67%
 40	  14460	  0.97%
 41	  19237	  1.29%
 42	  22975	  1.53%
 43	  16736	  1.12%
 44	  11606	  0.78%
 45	  15296	  1.02%
 46	  12227	  0.82%
 47	   9298	  0.62%
 48	    918	  0.06%
 49	   1440	  0.10%
 50	  74774	  5.00%


criterion=sequence-density
sequence-density=6.74
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.20
sequence-density-rank=5
fanout-score=21.54
fanout-score-rank=1
prefix-density=2.28
prefix-fanout=20.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGC
                                 Started job on |	Mar 04 07:07:47
                             Started mapping on |	Mar 04 07:07:48
                                    Finished on |	Mar 04 07:08:11
       Mapping speed, Million of reads per hour |	240.38

                          Number of input reads |	1535789
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	758537
                        Uniquely mapped reads % |	49.39%
                          Average mapped length |	25.01
                       Number of splices: Total |	878
            Number of splices: Annotated (sjdb) |	225
                       Number of splices: GT/AG |	831
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	34
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.79
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	518571
             % of reads mapped to multiple loci |	33.77%
        Number of reads mapped to too many loci |	47772
             % of reads mapped to too many loci |	3.11%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.03%
                     % of reads unmapped: other |	0.70%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	258681	258681	258681
N_multimapping	518571	518571	518571
N_noFeature	57673	67926	512099
N_ambiguous	420931	206981	9343
UnstrandedReadsAssigned:279933 PositiveStrandReadsAssigned:483630 NegativeStrandReadsAssigned:237095
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031019 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031019-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,535,789 reads, 381,335 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,124 rounds

 109283 SRR5031019.ke.tsv
  52636 SRR5031019.se.tsv
 161919 total
==> SRR5031019.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031019.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031019 completed mapping pipeline successfully
