Starting /dee2/code/volunteer_pipeline.sh SRR5030959
    current disk space = 160580304896
    free memory = 73947028 
SRR5030959 SRAfilesize
9fce01b119996ad8b7fe360b509ca103  SRR5030959.sra
SRR5030959.sra file validated
SRR5030959 is single end
SRR5030959 is conventional basespace
SRR5030959 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4335	34.0	31.0	34.0	31.0	34.0
2	32.73775	34.0	31.0	34.0	31.0	34.0
3	32.7485	34.0	31.0	34.0	31.0	34.0
4	36.2565	37.0	37.0	37.0	35.0	37.0
5	36.28875	37.0	37.0	37.0	35.0	37.0
6	36.11225	37.0	37.0	37.0	35.0	37.0
7	36.15	37.0	35.0	37.0	35.0	37.0
8	36.21025	37.0	36.0	37.0	35.0	37.0
9	37.96725	39.0	38.0	39.0	35.0	39.0
10	37.92175	39.0	38.0	39.0	35.0	39.0
11	37.83325	39.0	38.0	39.0	35.0	39.0
12	37.7315	39.0	38.0	39.0	35.0	39.0
13	37.8255	39.0	38.0	39.0	35.0	39.0
14	39.10475	40.0	39.0	41.0	36.0	41.0
15	39.22075	41.0	39.0	41.0	36.0	41.0
16	39.13625	40.0	39.0	41.0	36.0	41.0
17	39.1235	40.0	39.0	41.0	36.0	41.0
18	39.4035	41.0	39.0	41.0	36.0	41.0
19	39.2465	41.0	39.0	41.0	36.0	41.0
20	39.15725	41.0	39.0	41.0	36.0	41.0
21	39.07225	40.0	39.0	41.0	35.0	41.0
22	39.2455	41.0	39.0	41.0	36.0	41.0
23	39.226	41.0	39.0	41.0	36.0	41.0
24	38.458	40.0	38.0	41.0	34.0	41.0
25	38.7135	40.0	38.0	41.0	34.0	41.0
26	38.99425	40.0	39.0	41.0	35.0	41.0
27	39.017	41.0	39.0	41.0	35.0	41.0
28	38.71425	40.0	38.0	41.0	34.0	41.0
29	38.08975	40.0	38.0	41.0	33.0	41.0
30	38.3555	40.0	38.0	41.0	33.0	41.0
31	38.69875	40.0	38.0	41.0	34.0	41.0
32	38.62875	40.0	38.0	41.0	34.0	41.0
33	37.63775	40.0	37.0	41.0	32.0	41.0
34	37.24675	40.0	37.0	41.0	30.0	41.0
35	37.333	39.0	37.0	41.0	31.0	41.0
36	33.756	38.0	30.0	40.0	20.0	41.0
37	35.956	38.0	35.0	40.0	30.0	41.0
38	37.18625	39.0	37.0	40.0	32.0	41.0
39	37.35025	39.0	37.0	40.0	32.0	41.0
40	37.12	39.0	37.0	40.0	32.0	41.0
41	37.0045	39.0	36.0	40.0	32.0	41.0
42	36.979	39.0	36.0	40.0	31.0	41.0
43	36.89775	39.0	36.0	41.0	30.0	41.0
44	37.12825	40.0	37.0	41.0	32.0	41.0
45	36.67425	39.0	36.0	41.0	31.0	41.0
46	34.27925	38.0	33.0	40.0	24.0	41.0
47	35.22375	38.0	34.0	40.0	28.0	41.0
48	35.32925	38.0	35.0	40.0	28.0	41.0
49	34.75325	38.0	34.0	40.0	27.0	41.0
50	30.47375	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	1.0
14	1.0
15	0.0
16	1.0
17	0.0
18	3.0
19	2.0
20	4.0
21	6.0
22	4.0
23	8.0
24	17.0
25	15.0
26	16.0
27	31.0
28	36.0
29	38.0
30	62.0
31	81.0
32	97.0
33	106.0
34	146.0
35	195.0
36	312.0
37	542.0
38	989.0
39	1285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.623246492985972	25.37575150300601	45.44088176352705	17.560120240480963
2	21.0	26.55	19.225	33.225
3	27.700000000000003	18.75	22.225	31.324999999999996
4	34.775	26.625	15.425	23.175
5	19.725	40.875	23.275000000000002	16.125
6	25.974999999999998	16.55	43.75	13.725000000000001
7	23.974999999999998	18.0	20.075000000000003	37.95
8	21.875	30.025000000000002	15.925	32.175
9	27.425	29.125	27.0	16.45
10	25.85	17.4	33.225	23.525
11	30.075000000000003	16.825000000000003	16.5	36.6
12	16.025	22.275	33.35	28.349999999999998
13	30.3	17.549999999999997	29.025000000000002	23.125
14	25.7	35.65	21.075	17.575
15	38.525	22.85	23.849999999999998	14.774999999999999
16	16.3	20.4	25.6	37.7
17	23.1	22.900000000000002	37.8	16.2
18	23.799999999999997	16.35	37.8	22.05
19	35.699999999999996	15.775	31.2	17.325
20	42.55	17.2	24.175	16.075
21	23.150000000000002	20.525	25.324999999999996	31.0
22	30.175	14.825	43.125	11.875
23	29.95	14.075	39.525	16.45
24	47.625	18.325	22.75	11.3
25	45.975	24.9	15.2	13.925
26	26.650000000000002	42.025	17.75	13.575000000000001
27	17.05	42.15	25.674999999999997	15.125
28	14.274999999999999	22.475	45.35	17.9
29	11.025	14.224999999999998	53.974999999999994	20.775
30	11.725	9.35	33.1	45.824999999999996
31	19.3	7.5249999999999995	41.55	31.624999999999996
32	31.15	8.375	21.0	39.475
33	51.4	10.375	14.75	23.474999999999998
34	54.949999999999996	9.8	19.7	15.55
35	56.65	9.475	19.900000000000002	13.975000000000001
36	41.275	10.075000000000001	31.825	16.825000000000003
37	39.95	11.924999999999999	20.424999999999997	27.700000000000003
38	22.975	18.25	14.6	44.175
39	18.45	26.200000000000003	11.225	44.125
40	20.925	43.25	14.625	21.2
41	28.975	44.05	12.725	14.249999999999998
42	47.325	28.299999999999997	13.025	11.35
43	46.275	31.324999999999996	10.7	11.700000000000001
44	28.199999999999996	45.95	10.125	15.725
45	17.65	46.550000000000004	16.725	19.075
46	14.45	25.775	21.175	38.6
47	12.925	15.775	35.75	35.55
48	14.2	15.15	19.675	50.975
49	18.6	20.65	16.0	44.75
50	20.825	34.475	17.65	27.05
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	2.0
21	1.0
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	2.0
28	2.0
29	5.0
30	8.0
31	19.0
32	30.0
33	31.5
34	33.0
35	41.5
36	50.0
37	65.0
38	80.0
39	72.0
40	64.0
41	72.0
42	80.0
43	117.5
44	155.0
45	198.5
46	242.0
47	297.0
48	352.0
49	451.0
50	550.0
51	636.0
52	722.0
53	543.0
54	364.0
55	474.0
56	584.0
57	426.5
58	269.0
59	167.5
60	66.0
61	60.0
62	54.0
63	66.5
64	79.0
65	53.0
66	27.0
67	21.5
68	16.0
69	35.5
70	55.0
71	76.0
72	97.0
73	51.0
74	5.0
75	3.5
76	2.0
77	2.0
78	2.0
79	4.5
80	7.0
81	4.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95361781076066	30.3
2	9.461966604823747	7.6499999999999995
3	4.019789734075449	4.875
4	2.9684601113172544	4.8
5	1.4842300556586272	3.0
6	0.8039579468150896	1.95
7	0.9894867037724181	2.8000000000000003
8	0.6802721088435374	2.1999999999999997
9	0.4329004329004329	1.575
>10	3.772418058132344	24.7
>50	0.30921459492888065	9.175
>100	0.12368583797155226	6.9750000000000005
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	156	3.9	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	123	3.075	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	89	2.225	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	79	1.975	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	53	1.325	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	41	1.0250000000000001	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	39	0.975	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	36	0.8999999999999999	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	30	0.75	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	30	0.75	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	19	0.475	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	18	0.44999999999999996	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	16	0.4	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	16	0.4	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	15	0.375	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTC	15	0.375	RNA PCR Primer, Index 41 (100% over 44bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	14	0.35000000000000003	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	14	0.35000000000000003	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	12	0.3	RNA PCR Primer, Index 7 (97% over 35bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	12	0.3	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	12	0.3	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	11	0.27499999999999997	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	11	0.27499999999999997	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	11	0.27499999999999997	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	11	0.27499999999999997	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	11	0.27499999999999997	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	11	0.27499999999999997	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	10	0.25	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	10	0.25	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	10	0.25	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	10	0.25	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	8	0.2	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT	7	0.17500000000000002	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	7	0.17500000000000002	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 30bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	7	0.17500000000000002	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	5	0.125	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGCAACTCTGTGCTGAATGT	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTAT	5	0.125	RNA PCR Primer, Index 41 (100% over 48bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	5	0.125	No Hit
GCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACACT	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.075	0.0	0.0	0.0
2	0.0	0.075	0.0	0.0	0.0
3	0.0	0.2	0.0	0.0	0.0
4	0.0	0.2	0.0	0.0	0.0
5	0.0	0.2	0.0	0.0	0.0
6	0.0	0.225	0.0	0.0	0.0
7	0.0	0.625	0.0	0.0	0.0
8	0.0	0.75	0.0	0.0	0.0
9	0.0	0.75	0.0	0.0	0.0
10	0.0	0.8	0.0	0.0	0.0
11	0.0	0.875	0.0	0.0	0.0
12	0.0	0.95	0.0	0.0	0.0
13	0.0	1.05	0.0	0.0	0.0
14	0.0	1.225	0.0	0.0	0.0
15	0.0	1.45	0.0	0.0	0.0
16	0.0	2.2	0.0	0.0	0.0
17	0.0	2.8	0.0	0.0	0.0
18	0.0	3.875	0.0	0.0	0.0
19	0.0	4.375	0.0	0.0	0.0
20	0.0	5.5	0.0	0.0	0.0
21	0.0	12.275	0.0	0.0	0.0
22	0.0	22.775	0.0	0.0	0.0
23	0.0	47.075	0.0	0.0	0.0
24	0.0	57.225	0.0	0.0	0.0
25	0.0	61.525	0.0	0.0	0.0
26	0.0	63.0	0.0	0.0	0.0
27	0.0	63.625	0.0	0.0	0.0
28	0.0	64.2	0.0	0.0	0.0
29	0.0	64.7	0.0	0.0	0.0
30	0.0	64.95	0.0	0.0	0.0
31	0.0	65.475	0.0	0.0	0.0
32	0.0	66.425	0.0	0.0	0.0
33	0.0	67.475	0.0	0.0	0.0
34	0.0	69.4	0.0	0.0	0.0
35	0.0	70.6	0.0	0.0	0.0
36	0.0	71.45	0.0	0.0	0.0
37	0.0	72.25	0.0	0.0	0.0
38	0.0	72.7	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGGCT	30	1.9515464E-6	44.0	16
GCTTGGC	30	1.9515464E-6	44.0	15
CTGAGCT	60	1.8189894E-12	40.333332	11
AGCTTGG	60	1.8189894E-12	40.333332	14
GAGCTTG	60	1.8189894E-12	40.333332	13
GGATCCG	60	1.8189894E-12	40.333332	3
CCGTCTG	60	1.8189894E-12	40.333332	7
TCGGATC	60	1.8189894E-12	40.333332	1
CGGATCC	60	1.8189894E-12	40.333332	2
GATCCGT	60	1.8189894E-12	40.333332	4
TCCGTCT	60	1.8189894E-12	40.333332	6
ATCCGTC	60	1.8189894E-12	40.333332	5
TGAGCTT	60	1.8189894E-12	40.333332	12
CGTCTGA	60	1.8189894E-12	40.333332	8
CTTGGTG	30	1.083877E-4	36.666664	16
GCTTGGT	30	1.083877E-4	36.666664	15
TGGTGGA	30	1.083877E-4	36.666664	18
TTGGTGG	35	2.6843656E-4	31.428572	17
GGTGGAA	35	2.6843656E-4	31.428572	19
TCTGAGC	80	5.820766E-11	30.250002	10
>>END_MODULE
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
Read 233361 spots for SRR5030959.sra
Written 233361 spots for SRR5030959.sra
SRR ids: ['SRR5030959.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_wizhtbfv
SRR5030959.sra spots: 2800332
blocks: [[1, 233361], [233362, 466722], [466723, 700083], [700084, 933444], [933445, 1166805], [1166806, 1400166], [1400167, 1633527], [1633528, 1866888], [1866889, 2100249], [2100250, 2333610], [2333611, 2566971], [2566972, 2800332]]
SRR5030959 file size 484280
SRR5030959 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030959 SRR5030959_1.fastq
Input file:	SRR5030959_1.fastq
trimmed:	SRR5030959-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:54:17 2020 >> started

Wed Mar  4 06:54:19 2020 >> done (1.400s)
2800332 reads processed; of these:
    174 ( 0.01%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
2800155 (99.99%) reads available; of these:
 215046 ( 7.68%) trimmed reads available after processing
2585109 (92.32%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     32	  0.00%
 19	     62	  0.00%
 20	    162	  0.01%
 21	    130	  0.00%
 22	    264	  0.01%
 23	    336	  0.01%
 24	    378	  0.01%
 25	    438	  0.02%
 26	    440	  0.02%
 27	    566	  0.02%
 28	    597	  0.02%
 29	    914	  0.03%
 30	    694	  0.02%
 31	    910	  0.03%
 32	   1272	  0.05%
 33	   2379	  0.08%
 34	   6424	  0.23%
 35	   2944	  0.11%
 36	   1995	  0.07%
 37	   2353	  0.08%
 38	   3304	  0.12%
 39	   3680	  0.13%
 40	   5504	  0.20%
 41	   4188	  0.15%
 42	   7972	  0.28%
 43	   5944	  0.21%
 44	  14938	  0.53%
 45	  14972	  0.53%
 46	  14632	  0.52%
 47	  17540	  0.63%
 48	  38493	  1.37%
 49	  60589	  2.16%
 50	2585109	 92.32%
2800155 reads passed initial QC


criterion=sequence-density
sequence-density=71.71
sequence-density-rank=1
fanout-score=21.02
fanout-score-rank=2
prefix-density=74.65
prefix-fanout=20.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC


criterion=fanout-score
sequence-density=1.97
sequence-density-rank=5
fanout-score=123.37
fanout-score-rank=1
prefix-density=73.99
prefix-fanout=3.3
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC -o SRR5030959 -
Input file:	STDIN
trimmed:	SRR5030959-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:54:32 2020 >> started

Wed Mar  4 06:54:35 2020 >> done (3.006s)
2722373 reads processed; of these:
 118575 ( 4.36%) short reads filtered out after trimming by size control
   6575 ( 0.24%) empty reads filtered out after trimming by size control
2597223 (95.40%) reads available; of these:
2151504 (82.84%) trimmed reads available after processing
 445719 (17.16%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  11238	  0.43%
 19	  21261	  0.82%
 20	 165519	  6.37%
 21	 273548	 10.53%
 22	 685699	 26.40%
 23	 283012	 10.90%
 24	 130260	  5.02%
 25	  36840	  1.42%
 26	  18726	  0.72%
 27	  14057	  0.54%
 28	  16887	  0.65%
 29	  12073	  0.46%
 30	  18659	  0.72%
 31	  29115	  1.12%
 32	  27253	  1.05%
 33	  49948	  1.92%
 34	  26592	  1.02%
 35	  22276	  0.86%
 36	  21979	  0.85%
 37	  16124	  0.62%
 38	  18470	  0.71%
 39	  18858	  0.73%
 40	  30270	  1.17%
 41	  39257	  1.51%
 42	  45092	  1.74%
 43	  32223	  1.24%
 44	  24276	  0.93%
 45	  38359	  1.48%
 46	  30737	  1.18%
 47	  20828	  0.80%
 48	   4784	  0.18%
 49	  14457	  0.56%
 50	 398546	 15.35%


criterion=sequence-density
sequence-density=6.59
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.78
sequence-density-rank=15
fanout-score=13.15
fanout-score-rank=1
prefix-density=10.28
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:54:56
                             Started mapping on |	Mar 04 06:54:56
                                    Finished on |	Mar 04 06:55:18
       Mapping speed, Million of reads per hour |	437.73

                          Number of input reads |	2675005
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1620780
                        Uniquely mapped reads % |	60.59%
                          Average mapped length |	28.92
                       Number of splices: Total |	1662
            Number of splices: Annotated (sjdb) |	320
                       Number of splices: GT/AG |	1521
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	124
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.60
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	675759
             % of reads mapped to multiple loci |	25.26%
        Number of reads mapped to too many loci |	58059
             % of reads mapped to too many loci |	2.17%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.14%
                     % of reads unmapped: other |	0.83%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	378466	378466	378466
N_multimapping	675759	675759	675759
N_noFeature	68287	95532	1068293
N_ambiguous	903398	420665	21800
UnstrandedReadsAssigned:649095 PositiveStrandReadsAssigned:1104583 NegativeStrandReadsAssigned:530687
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030959 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030959-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,675,005 reads, 726,547 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,103 rounds

 109283 SRR5030959.ke.tsv
  52636 SRR5030959.se.tsv
 161919 total
==> SRR5030959.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030959.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030959 completed mapping pipeline successfully
