Starting /dee2/code/volunteer_pipeline.sh SRR5030940
    current disk space = 160608190464
    free memory = 73912876 
SRR5030940 SRAfilesize
b7cd962c7cd96706254b5dae55a123ac  SRR5030940.sra
SRR5030940.sra file validated
SRR5030940 is single end
SRR5030940 is conventional basespace
SRR5030940 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4045	33.0	31.0	34.0	31.0	34.0
2	32.678	34.0	31.0	34.0	31.0	34.0
3	32.79925	34.0	31.0	34.0	31.0	34.0
4	36.22325	37.0	37.0	37.0	35.0	37.0
5	36.248	37.0	37.0	37.0	35.0	37.0
6	36.0945	37.0	36.0	37.0	35.0	37.0
7	36.0465	37.0	35.0	37.0	35.0	37.0
8	36.14775	37.0	36.0	37.0	35.0	37.0
9	37.9595	39.0	38.0	39.0	35.0	39.0
10	37.93425	39.0	38.0	39.0	35.0	39.0
11	37.762	39.0	38.0	39.0	35.0	39.0
12	37.83	39.0	38.0	39.0	35.0	39.0
13	37.72225	39.0	38.0	39.0	35.0	39.0
14	39.16825	41.0	39.0	41.0	36.0	41.0
15	39.1645	41.0	39.0	41.0	36.0	41.0
16	39.16775	41.0	39.0	41.0	36.0	41.0
17	39.2855	41.0	39.0	41.0	36.0	41.0
18	39.24375	41.0	39.0	41.0	36.0	41.0
19	39.10025	41.0	39.0	41.0	35.0	41.0
20	39.04475	40.0	39.0	41.0	35.0	41.0
21	39.1585	40.0	39.0	41.0	36.0	41.0
22	39.13625	41.0	39.0	41.0	36.0	41.0
23	38.93525	40.0	39.0	41.0	35.0	41.0
24	38.7985	40.0	38.0	41.0	35.0	41.0
25	39.1005	41.0	39.0	41.0	35.0	41.0
26	39.12925	41.0	39.0	41.0	35.0	41.0
27	39.17675	41.0	39.0	41.0	36.0	41.0
28	38.81925	40.0	38.0	41.0	35.0	41.0
29	38.17925	40.0	38.0	41.0	33.0	41.0
30	38.4765	40.0	38.0	41.0	34.0	41.0
31	38.7995	40.0	38.0	41.0	35.0	41.0
32	39.07575	41.0	39.0	41.0	35.0	41.0
33	38.27525	40.0	38.0	41.0	34.0	41.0
34	38.13075	40.0	38.0	41.0	33.0	41.0
35	37.7185	39.0	37.0	40.0	33.0	41.0
36	33.63875	38.0	30.0	40.0	18.0	41.0
37	35.22225	38.0	33.0	40.0	27.0	41.0
38	36.90175	39.0	36.0	40.0	31.0	41.0
39	36.731	39.0	36.0	40.0	31.0	41.0
40	36.879	39.0	36.0	40.0	31.0	41.0
41	37.01525	39.0	36.0	40.0	31.0	41.0
42	37.1025	40.0	37.0	41.0	31.0	41.0
43	36.88475	39.0	36.0	41.0	31.0	41.0
44	36.77425	39.0	36.0	41.0	31.0	41.0
45	36.51625	39.0	36.0	40.0	30.0	41.0
46	34.8585	38.0	34.0	40.0	24.0	41.0
47	35.646	39.0	35.0	40.0	28.0	41.0
48	35.90375	39.0	35.0	40.0	30.0	41.0
49	34.747	38.0	34.0	40.0	26.0	41.0
50	30.05325	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	3.0
20	4.0
21	3.0
22	3.0
23	9.0
24	13.0
25	13.0
26	30.0
27	25.0
28	37.0
29	45.0
30	46.0
31	62.0
32	85.0
33	114.0
34	170.0
35	236.0
36	290.0
37	526.0
38	1027.0
39	1253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.270541082164328	23.371743486973948	51.57815631262525	14.779559118236474
2	20.075000000000003	27.075	20.25	32.6
3	25.5	17.125	20.95	36.425000000000004
4	31.900000000000002	28.225	13.275	26.6
5	20.275000000000002	40.775	23.150000000000002	15.8
6	30.325000000000003	17.625	38.675	13.375
7	18.625	20.5	24.25	36.625
8	22.625	32.425	14.95	30.0
9	26.325	33.0	26.674999999999997	14.000000000000002
10	23.375	18.175	32.65	25.8
11	27.675	15.625	15.775	40.925
12	13.950000000000001	20.175	33.5	32.375
13	28.499999999999996	19.875	27.700000000000003	23.925
14	27.900000000000002	33.525	20.7	17.875
15	36.525	23.575	23.875	16.025
16	16.175	24.175	21.25	38.4
17	22.05	21.8	39.625	16.525000000000002
18	22.375	17.25	36.075	24.3
19	36.125	15.2	30.375000000000004	18.3
20	42.825	15.85	26.125	15.2
21	23.075000000000003	18.575	26.625	31.724999999999998
22	27.375	14.549999999999999	47.25	10.825
23	28.749999999999996	13.25	43.75	14.249999999999998
24	51.65	15.575	23.799999999999997	8.975
25	49.475	24.725	14.7	11.1
26	26.025	48.199999999999996	15.0	10.775
27	16.025	46.975	26.325	10.674999999999999
28	12.475	23.325000000000003	49.525000000000006	14.674999999999999
29	9.55	12.825000000000001	56.675	20.95
30	10.525	7.7	35.925000000000004	45.85
31	15.25	6.0	45.675	33.074999999999996
32	28.299999999999997	7.825	20.4	43.475
33	56.89999999999999	8.55	13.65	20.9
34	62.175000000000004	7.9	16.025	13.900000000000002
35	61.625	7.675	19.7	11.0
36	43.075	9.625	33.7	13.600000000000001
37	45.5	9.475	18.55	26.474999999999998
38	21.75	15.975	12.575	49.7
39	16.075	26.55	8.9	48.475
40	16.2	50.05	11.15	22.6
41	26.724999999999998	47.8	9.325	16.150000000000002
42	52.55	29.099999999999998	9.25	9.1
43	49.3	33.775	8.55	8.375
44	26.5	51.425	7.95	14.124999999999998
45	16.400000000000002	50.24999999999999	13.65	19.7
46	11.675	26.325	19.8	42.199999999999996
47	8.924999999999999	16.0	38.5	36.575
48	9.625	14.000000000000002	19.25	57.125
49	15.1	21.3	14.025000000000002	49.575
50	20.9	37.425000000000004	13.700000000000001	27.975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	4.0
28	6.0
29	5.0
30	4.0
31	10.0
32	16.0
33	17.0
34	18.0
35	27.5
36	37.0
37	42.0
38	47.0
39	54.5
40	62.0
41	66.5
42	71.0
43	105.0
44	139.0
45	160.0
46	181.0
47	298.0
48	415.0
49	513.5
50	612.0
51	709.5
52	807.0
53	629.5
54	452.0
55	477.5
56	503.0
57	422.0
58	341.0
59	209.0
60	77.0
61	60.5
62	44.0
63	60.5
64	77.0
65	54.5
66	32.0
67	21.0
68	10.0
69	10.0
70	10.0
71	14.0
72	18.0
73	12.0
74	6.0
75	4.5
76	3.0
77	2.5
78	2.0
79	3.0
80	4.0
81	2.5
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.099999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63503649635037	30.675
2	10.523114355231144	8.649999999999999
3	4.197080291970803	5.175
4	2.3722627737226274	3.9
5	1.094890510948905	2.25
6	1.5206812652068127	3.75
7	0.6082725060827251	1.7500000000000002
8	0.48661800486618007	1.6
9	0.6690997566909975	2.475
>10	3.345498783454988	21.2
>50	0.36496350364963503	9.925
>100	0.18248175182481752	8.649999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	139	3.4750000000000005	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	106	2.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	75	1.875	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	21	0.525	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	18	0.44999999999999996	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC	18	0.44999999999999996	RNA PCR Primer, Index 47 (100% over 44bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	17	0.42500000000000004	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	16	0.4	RNA PCR Primer, Index 5 (97% over 35bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	16	0.4	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	10	0.25	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	10	0.25	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	9	0.22499999999999998	No Hit
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	8	0.2	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 47 (100% over 50bp)
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 33bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	7	0.17500000000000002	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA	7	0.17500000000000002	RNA PCR Primer, Index 47 (100% over 37bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	6	0.15	RNA PCR Primer, Index 3 (100% over 34bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	6	0.15	No Hit
CTAGACTGAGGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	6	0.15	RNA PCR Primer, Index 5 (97% over 35bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	6	0.15	No Hit
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.3	0.0	0.0	0.0
3	0.0	0.45	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.575	0.0	0.0	0.0
7	0.0	1.125	0.0	0.0	0.0
8	0.0	1.225	0.0	0.0	0.0
9	0.0	1.3	0.0	0.0	0.0
10	0.0	1.45	0.0	0.0	0.0
11	0.0	1.75	0.0	0.0	0.0
12	0.0	2.0	0.0	0.0	0.0
13	0.0	2.175	0.0	0.0	0.0
14	0.0	2.575	0.0	0.0	0.0
15	0.0	3.1	0.0	0.0	0.0
16	0.0	4.175	0.0	0.0	0.0
17	0.0	4.575	0.0	0.0	0.0
18	0.0	5.5	0.0	0.0	0.0
19	0.0	6.175	0.0	0.0	0.0
20	0.0	7.05	0.0	0.0	0.0
21	0.0	12.975	0.0	0.0	0.0
22	0.0	25.775	0.0	0.0	0.0
23	0.0	55.225	0.0	0.0	0.0
24	0.0	66.475	0.0	0.0	0.0
25	0.0	72.075	0.0	0.0	0.0
26	0.0	73.7	0.0	0.0	0.0
27	0.0	74.3	0.0	0.0	0.0
28	0.0	74.925	0.0	0.0	0.0
29	0.0	75.475	0.0	0.0	0.0
30	0.0	75.75	0.0	0.0	0.0
31	0.0	76.5	0.0	0.0	0.0
32	0.0	77.6	0.0	0.0	0.0
33	0.0	78.4	0.0	0.0	0.0
34	0.0	79.825	0.0	0.0	0.0
35	0.0	80.85	0.0	0.0	0.0
36	0.0	81.75	0.0	0.0	0.0
37	0.0	82.3	0.0	0.0	0.0
38	0.0	82.875	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCTG	20	6.952051E-4	44.000004	1
CTGAGCT	30	1.9515464E-6	44.0	11
AGCTTGG	30	1.9515464E-6	44.0	14
GAGCTTG	30	1.9515464E-6	44.0	13
GGATCCG	30	1.9515464E-6	44.0	3
CCGTCTG	30	1.9515464E-6	44.0	7
TCGGATC	30	1.9515464E-6	44.0	1
CGGATCC	30	1.9515464E-6	44.0	2
CTTGGCT	25	3.6958358E-5	44.0	16
GATCCGT	30	1.9515464E-6	44.0	4
GCTTGGC	25	3.6958358E-5	44.0	15
TCCGTCT	30	1.9515464E-6	44.0	6
ATCCGTC	30	1.9515464E-6	44.0	5
TGAGCTT	30	1.9515464E-6	44.0	12
CGTCTGA	30	1.9515464E-6	44.0	8
TGTTGGA	25	0.002084305	35.2	20
AATTTGT	25	0.002084305	35.2	16
CCTGTAG	25	0.002084305	35.2	4
GCTGGAA	25	0.002084305	35.2	20
CGAATTT	25	0.002084305	35.2	14
>>END_MODULE
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203261 spots for SRR5030940.sra
Written 203261 spots for SRR5030940.sra
Read 203268 spots for SRR5030940.sra
Written 203268 spots for SRR5030940.sra
SRR ids: ['SRR5030940.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8ffndcj4
SRR5030940.sra spots: 2439139
blocks: [[1, 203261], [203262, 406522], [406523, 609783], [609784, 813044], [813045, 1016305], [1016306, 1219566], [1219567, 1422827], [1422828, 1626088], [1626089, 1829349], [1829350, 2032610], [2032611, 2235871], [2235872, 2439139]]
SRR5030940 file size 421679
SRR5030940 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030940 SRR5030940_1.fastq
Input file:	SRR5030940_1.fastq
trimmed:	SRR5030940-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:50:26 2020 >> started

Wed Mar  4 06:50:27 2020 >> done (1.674s)
2439139 reads processed; of these:
    131 ( 0.01%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
2439003 (99.99%) reads available; of these:
 142674 ( 5.85%) trimmed reads available after processing
2296329 (94.15%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     37	  0.00%
 19	     46	  0.00%
 20	    105	  0.00%
 21	    123	  0.01%
 22	    214	  0.01%
 23	    262	  0.01%
 24	    272	  0.01%
 25	    325	  0.01%
 26	    276	  0.01%
 27	    404	  0.02%
 28	    483	  0.02%
 29	    707	  0.03%
 30	    548	  0.02%
 31	    717	  0.03%
 32	    619	  0.03%
 33	   1016	  0.04%
 34	   2454	  0.10%
 35	   2406	  0.10%
 36	   1700	  0.07%
 37	   1859	  0.08%
 38	   1972	  0.08%
 39	   2106	  0.09%
 40	   2414	  0.10%
 41	   2682	  0.11%
 42	   3489	  0.14%
 43	   4648	  0.19%
 44	   8646	  0.35%
 45	  10005	  0.41%
 46	   9572	  0.39%
 47	  13226	  0.54%
 48	  28938	  1.19%
 49	  40403	  1.66%
 50	2296329	 94.15%
2439003 reads passed initial QC


criterion=sequence-density
sequence-density=82.63
sequence-density-rank=1
fanout-score=18.90
fanout-score-rank=1
prefix-density=84.43
prefix-fanout=18.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC


criterion=fanout-score
sequence-density=82.63
sequence-density-rank=1
fanout-score=18.90
fanout-score-rank=1
prefix-density=84.43
prefix-fanout=18.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC -o SRR5030940 -
Input file:	STDIN
trimmed:	SRR5030940-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:50:46 2020 >> started

Wed Mar  4 06:50:52 2020 >> done (5.269s)
2380232 reads processed; of these:
 136063 ( 5.72%) short reads filtered out after trimming by size control
   9345 ( 0.39%) empty reads filtered out after trimming by size control
2234824 (93.89%) reads available; of these:
2010713 (89.97%) trimmed reads available after processing
 224111 (10.03%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13718	  0.61%
 19	  19218	  0.86%
 20	 145789	  6.52%
 21	 281238	 12.58%
 22	 716796	 32.07%
 23	 273995	 12.26%
 24	 141085	  6.31%
 25	  38908	  1.74%
 26	  17411	  0.78%
 27	  13554	  0.61%
 28	  14475	  0.65%
 29	  10569	  0.47%
 30	  16176	  0.72%
 31	  28788	  1.29%
 32	  19623	  0.88%
 33	  45898	  2.05%
 34	  19463	  0.87%
 35	  15838	  0.71%
 36	  15744	  0.70%
 37	  10623	  0.48%
 38	  11366	  0.51%
 39	  12226	  0.55%
 40	  18492	  0.83%
 41	  22606	  1.01%
 42	  25562	  1.14%
 43	  18093	  0.81%
 44	  14963	  0.67%
 45	  19333	  0.87%
 46	  13139	  0.59%
 47	  10198	  0.46%
 48	   2399	  0.11%
 49	   8570	  0.38%
 50	 198968	  8.90%


criterion=sequence-density
sequence-density=5.13
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTT


criterion=fanout-score
sequence-density=0.57
sequence-density-rank=26
fanout-score=18.86
fanout-score-rank=1
prefix-density=10.65
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:51:21
                             Started mapping on |	Mar 04 06:51:22
                                    Finished on |	Mar 04 06:51:45
       Mapping speed, Million of reads per hour |	359.00

                          Number of input reads |	2293595
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1443480
                        Uniquely mapped reads % |	62.94%
                          Average mapped length |	26.48
                       Number of splices: Total |	987
            Number of splices: Annotated (sjdb) |	207
                       Number of splices: GT/AG |	898
                       Number of splices: GC/AG |	24
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	65
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.50
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	622315
             % of reads mapped to multiple loci |	27.13%
        Number of reads mapped to too many loci |	41231
             % of reads mapped to too many loci |	1.80%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.37%
                     % of reads unmapped: other |	0.76%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	227800	227800	227800
N_multimapping	622315	622315	622315
N_noFeature	61901	76594	952905
N_ambiguous	820607	386131	11384
UnstrandedReadsAssigned:560972 PositiveStrandReadsAssigned:980755 NegativeStrandReadsAssigned:479191
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030940 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030940-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,293,595 reads, 667,118 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,099 rounds

 109283 SRR5030940.ke.tsv
  52636 SRR5030940.se.tsv
 161919 total
==> SRR5030940.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030940.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030940 completed mapping pipeline successfully
