Starting /dee2/code/volunteer_pipeline.sh SRR5031006
    current disk space = 160548667392
    free memory = 73950664 
SRR5031006 SRAfilesize
c1139d096609f53000eb2453f9a2f12d  SRR5031006.sra
SRR5031006.sra file validated
SRR5031006 is single end
SRR5031006 is conventional basespace
SRR5031006 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.265	33.0	31.0	34.0	31.0	34.0
2	32.571	34.0	31.0	34.0	31.0	34.0
3	32.60975	34.0	31.0	34.0	31.0	34.0
4	36.1535	37.0	35.0	37.0	35.0	37.0
5	36.0475	37.0	35.0	37.0	35.0	37.0
6	35.962	37.0	35.0	37.0	35.0	37.0
7	35.8625	37.0	35.0	37.0	35.0	37.0
8	35.91625	37.0	35.0	37.0	35.0	37.0
9	37.64625	39.0	37.0	39.0	35.0	39.0
10	37.563	39.0	37.0	39.0	35.0	39.0
11	37.194	39.0	37.0	39.0	33.0	39.0
12	37.5905	39.0	37.0	39.0	35.0	39.0
13	37.55875	39.0	37.0	39.0	35.0	39.0
14	38.88225	40.0	38.0	41.0	35.0	41.0
15	38.85575	40.0	38.0	41.0	35.0	41.0
16	38.959	40.0	38.0	41.0	35.0	41.0
17	39.0465	40.0	38.0	41.0	35.0	41.0
18	39.0275	40.0	38.0	41.0	35.0	41.0
19	38.869	40.0	38.0	41.0	35.0	41.0
20	38.8	40.0	38.0	41.0	35.0	41.0
21	38.87775	40.0	38.0	41.0	35.0	41.0
22	38.86725	40.0	38.0	41.0	35.0	41.0
23	38.4995	40.0	38.0	41.0	34.0	41.0
24	38.6975	40.0	38.0	41.0	34.0	41.0
25	38.8605	40.0	38.0	41.0	35.0	41.0
26	38.67	40.0	38.0	41.0	34.0	41.0
27	38.818	40.0	38.0	41.0	35.0	41.0
28	38.51975	40.0	38.0	41.0	34.0	41.0
29	38.2945	40.0	38.0	41.0	33.0	41.0
30	38.14125	40.0	38.0	41.0	33.0	41.0
31	38.36125	40.0	38.0	41.0	34.0	41.0
32	38.327	40.0	38.0	41.0	33.0	41.0
33	37.9095	40.0	38.0	41.0	33.0	41.0
34	37.81525	40.0	38.0	41.0	33.0	41.0
35	37.6785	40.0	37.0	41.0	33.0	41.0
36	37.64625	40.0	37.0	41.0	33.0	41.0
37	37.2195	39.0	37.0	41.0	31.0	41.0
38	37.07175	39.0	36.0	41.0	31.0	41.0
39	37.61325	40.0	37.0	41.0	33.0	41.0
40	37.758	40.0	37.0	41.0	33.0	41.0
41	37.97325	40.0	38.0	41.0	33.0	41.0
42	37.69825	40.0	37.0	41.0	33.0	41.0
43	37.84275	40.0	37.0	41.0	33.0	41.0
44	37.7625	40.0	37.0	41.0	33.0	41.0
45	37.7535	40.0	37.0	41.0	33.0	41.0
46	37.45025	40.0	37.0	41.0	32.0	41.0
47	36.88475	40.0	36.0	41.0	31.0	41.0
48	36.6675	39.0	36.0	41.0	30.0	41.0
49	36.786	40.0	36.0	41.0	31.0	41.0
50	36.00425	39.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	2.0
17	1.0
18	3.0
19	6.0
20	5.0
21	5.0
22	5.0
23	3.0
24	4.0
25	12.0
26	21.0
27	20.0
28	31.0
29	35.0
30	48.0
31	67.0
32	72.0
33	110.0
34	157.0
35	199.0
36	283.0
37	432.0
38	806.0
39	1672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.275	24.725	49.225	14.774999999999999
2	21.312296518908088	26.897069872276486	21.387427998998245	30.40320560981718
3	25.224999999999998	17.65	23.525	33.6
4	34.75	29.675	14.85	20.724999999999998
5	21.3	37.175000000000004	27.6	13.925
6	30.25	17.2	37.375	15.174999999999999
7	19.525000000000002	20.0	21.3	39.175
8	21.95	29.45	17.65	30.95
9	22.925	33.35	26.650000000000002	17.075000000000003
10	23.849999999999998	18.375	29.775000000000002	28.000000000000004
11	30.325000000000003	13.3	17.525	38.85
12	17.125	20.075000000000003	31.225	31.574999999999996
13	29.125	18.4	31.6	20.875
14	30.15	29.9	21.2	18.75
15	36.25	21.875	25.6	16.275000000000002
16	18.6	19.75	23.25	38.4
17	26.150000000000002	22.1	36.975	14.774999999999999
18	26.35	17.599999999999998	31.175000000000004	24.875
19	35.375	14.575	33.050000000000004	17.0
20	43.05	16.8	25.15	15.0
21	24.625	22.625	23.3	29.45
22	28.075	15.55	45.5	10.875
23	24.4	14.975	44.55	16.075
24	48.425000000000004	14.674999999999999	26.575	10.325
25	49.7	22.575	16.85	10.875
26	28.175	44.725	14.7	12.4
27	17.1	44.95	23.599999999999998	14.35
28	13.275	24.474999999999998	46.575	15.675
29	14.025000000000002	12.45	53.725	19.8
30	11.25	7.6499999999999995	36.025	45.074999999999996
31	15.075	5.975	44.1	34.849999999999994
32	26.75	7.025	23.375	42.85
33	52.5	8.525	14.424999999999999	24.55
34	59.52499999999999	8.674999999999999	15.4	16.400000000000002
35	61.1	9.025	19.125	10.75
36	41.325	9.9	34.825	13.950000000000001
37	44.525	12.049999999999999	21.45	21.975
38	25.674999999999997	16.325	12.8	45.2
39	16.3	24.2	10.174999999999999	49.325
40	15.0	47.475	12.425	25.1
41	23.474999999999998	47.199999999999996	11.325000000000001	18.0
42	46.7	28.625	12.950000000000001	11.725
43	50.349999999999994	28.199999999999996	9.700000000000001	11.75
44	31.374999999999996	45.95	8.125	14.549999999999999
45	19.45	48.125	13.425	19.0
46	15.525	25.874999999999996	18.7	39.900000000000006
47	10.75	15.85	37.275000000000006	36.125
48	11.675	12.55	21.875	53.900000000000006
49	14.149999999999999	19.950000000000003	15.1	50.8
50	18.75	36.575	13.350000000000001	31.324999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	1.0
24	2.0
25	1.5
26	1.0
27	2.5
28	4.0
29	3.5
30	3.0
31	9.0
32	15.0
33	15.0
34	15.0
35	26.5
36	38.0
37	36.0
38	34.0
39	35.5
40	37.0
41	48.0
42	59.0
43	82.5
44	106.0
45	125.5
46	145.0
47	282.5
48	420.0
49	537.0
50	654.0
51	760.5
52	867.0
53	664.0
54	461.0
55	497.0
56	533.0
57	383.0
58	233.0
59	152.5
60	72.0
61	57.0
62	42.0
63	80.5
64	119.0
65	77.0
66	35.0
67	26.0
68	17.0
69	19.5
70	22.0
71	36.5
72	51.0
73	28.5
74	6.0
75	3.0
76	0.0
77	1.5
78	3.0
79	2.5
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.074999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6001279590531	29.15
2	9.724888035828535	7.6
3	4.926423544465771	5.775
4	2.239283429302623	3.5000000000000004
5	1.599488163787588	3.125
6	1.0236724248240563	2.4
7	0.8957133717210494	2.45
8	0.8317338451695457	2.6
9	0.5758157389635317	2.025
>10	3.071017274472169	22.6
>50	0.3838771593090211	12.55
>100	0.12795905310300704	6.225
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	137	3.4250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	112	2.8000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	100	2.5	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	91	2.275	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	88	2.1999999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	85	2.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	55	1.375	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	39	0.975	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC	34	0.8500000000000001	RNA PCR Primer, Index 26 (100% over 50bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	30	0.75	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 35bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	26	0.65	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC	19	0.475	RNA PCR Primer, Index 26 (100% over 44bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	13	0.325	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	12	0.3	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	12	0.3	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	10	0.25	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	8	0.2	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	8	0.2	No Hit
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	7	0.17500000000000002	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	7	0.17500000000000002	RNA PCR Primer, Index 1 (97% over 37bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	6	0.15	RNA PCR Primer, Index 1 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
AAGAAAGATTGCAAGAACTGCTAATTCATGCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGCC	5	0.125	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG	5	0.125	RNA PCR Primer, Index 15 (100% over 36bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGAAAGATTGCAAGAACTGCTAATTCTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.925	0.0	0.0	0.0
2	0.0	0.95	0.0	0.0	0.0
3	0.0	1.05	0.0	0.0	0.0
4	0.0	1.1	0.0	0.0	0.0
5	0.0	1.125	0.0	0.0	0.0
6	0.0	1.3	0.0	0.0	0.0
7	0.0	1.875	0.0	0.0	0.0
8	0.0	2.075	0.0	0.0	0.0
9	0.0	2.125	0.0	0.0	0.0
10	0.0	2.275	0.0	0.0	0.0
11	0.0	2.45	0.0	0.0	0.0
12	0.0	2.725	0.0	0.0	0.0
13	0.0	3.075	0.0	0.0	0.0
14	0.0	3.425	0.0	0.0	0.0
15	0.0	4.025	0.0	0.0	0.0
16	0.0	5.275	0.0	0.0	0.0
17	0.0	6.275	0.0	0.0	0.0
18	0.0	7.65	0.0	0.0	0.0
19	0.0	8.325	0.0	0.0	0.0
20	0.0	9.325	0.0	0.0	0.0
21	0.0	14.15	0.0	0.0	0.0
22	0.0	24.55	0.0	0.0	0.0
23	0.0	52.0	0.0	0.0	0.0
24	0.0	64.55	0.0	0.0	0.0
25	0.0	70.2	0.0	0.0	0.0
26	0.0	71.65	0.0	0.0	0.0
27	0.0	72.525	0.0	0.0	0.0
28	0.0	73.05	0.0	0.0	0.0
29	0.0	73.725	0.0	0.0	0.0
30	0.0	74.15	0.0	0.0	0.0
31	0.0	74.775	0.0	0.0	0.0
32	0.0	76.675	0.0	0.0	0.0
33	0.0	77.8	0.0	0.0	0.0
34	0.0	80.725	0.0	0.0	0.0
35	0.0	82.225	0.0	0.0	0.0
36	0.0	83.6	0.0	0.0	0.0
37	0.0	85.0	0.0	0.0	0.0
38	0.0	85.625	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGAAC	25	3.4343255E-5	44.531647	1
TCGGATC	55	0.0	44.531647	1
ACATTCA	50	1.2732926E-11	44.531647	2
TTGAACC	25	3.4343255E-5	44.531647	2
AACATTC	50	1.2732926E-11	44.531647	1
CTCGACT	25	3.7082697E-5	43.975002	13
AACGCTG	50	1.4551915E-11	43.975002	8
ACGCTGT	50	1.4551915E-11	43.975002	9
ACTCGAC	25	3.7082697E-5	43.975002	12
TCGACTC	25	3.7082697E-5	43.975002	14
TTCAACG	50	1.4551915E-11	43.975002	5
ACCATCA	25	3.7082697E-5	43.975002	6
TCGGTGA	45	2.746674E-10	43.975002	15
CATTCAA	50	1.4551915E-11	43.975002	3
TCAACGC	50	1.4551915E-11	43.975002	6
CATCACT	25	3.7082697E-5	43.975002	8
AACCATC	25	3.7082697E-5	43.975002	5
GCTGTCG	50	1.4551915E-11	43.975002	11
GAACCAT	25	3.7082697E-5	43.975002	4
CCATCAC	25	3.7082697E-5	43.975002	7
>>END_MODULE
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197019 spots for SRR5031006.sra
Written 197019 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
Read 197017 spots for SRR5031006.sra
Written 197017 spots for SRR5031006.sra
SRR ids: ['SRR5031006.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_f7dhfkro
SRR5031006.sra spots: 2364206
blocks: [[1, 197017], [197018, 394034], [394035, 591051], [591052, 788068], [788069, 985085], [985086, 1182102], [1182103, 1379119], [1379120, 1576136], [1576137, 1773153], [1773154, 1970170], [1970171, 2167187], [2167188, 2364206]]
SRR5031006 file size 408696
SRR5031006 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031006 SRR5031006_1.fastq
Input file:	SRR5031006_1.fastq
trimmed:	SRR5031006-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:04:48 2020 >> started

Wed Mar  4 07:04:49 2020 >> done (1.574s)
2364206 reads processed; of these:
    271 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
2363931 (99.99%) reads available; of these:
 142300 ( 6.02%) trimmed reads available after processing
2221631 (93.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     88	  0.00%
 19	     70	  0.00%
 20	    189	  0.01%
 21	    207	  0.01%
 22	    332	  0.01%
 23	    429	  0.02%
 24	    443	  0.02%
 25	    534	  0.02%
 26	    430	  0.02%
 27	    570	  0.02%
 28	    645	  0.03%
 29	   1048	  0.04%
 30	    673	  0.03%
 31	    747	  0.03%
 32	    842	  0.04%
 33	   1018	  0.04%
 34	   1213	  0.05%
 35	   2101	  0.09%
 36	   1983	  0.08%
 37	   2194	  0.09%
 38	   2249	  0.10%
 39	   2425	  0.10%
 40	   2670	  0.11%
 41	   2943	  0.12%
 42	   3707	  0.16%
 43	   5643	  0.24%
 44	   7906	  0.33%
 45	   9619	  0.41%
 46	  13697	  0.58%
 47	  16696	  0.71%
 48	  25586	  1.08%
 49	  33403	  1.41%
 50	2221631	 93.98%
2363931 reads passed initial QC


criterion=sequence-density
sequence-density=85.41
sequence-density-rank=1
fanout-score=21.89
fanout-score-rank=1
prefix-density=87.35
prefix-fanout=21.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC


criterion=fanout-score
sequence-density=85.41
sequence-density-rank=1
fanout-score=21.89
fanout-score-rank=1
prefix-density=87.35
prefix-fanout=21.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC -o SRR5031006 -
Input file:	STDIN
trimmed:	SRR5031006-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:05:06 2020 >> started

Wed Mar  4 07:05:09 2020 >> done (3.045s)
2308956 reads processed; of these:
 156779 ( 6.79%) short reads filtered out after trimming by size control
  23189 ( 1.00%) empty reads filtered out after trimming by size control
2128988 (92.21%) reads available; of these:
2021106 (94.93%) trimmed reads available after processing
 107882 ( 5.07%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12553	  0.59%
 19	  22719	  1.07%
 20	 118722	  5.58%
 21	 243778	 11.45%
 22	 625617	 29.39%
 23	 286101	 13.44%
 24	 138796	  6.52%
 25	  40145	  1.89%
 26	  20796	  0.98%
 27	  16732	  0.79%
 28	  17302	  0.81%
 29	  13730	  0.64%
 30	  20627	  0.97%
 31	  44821	  2.11%
 32	  28107	  1.32%
 33	  74232	  3.49%
 34	  35217	  1.65%
 35	  31915	  1.50%
 36	  36665	  1.72%
 37	  14825	  0.70%
 38	  17011	  0.80%
 39	  14300	  0.67%
 40	  21500	  1.01%
 41	  25119	  1.18%
 42	  29585	  1.39%
 43	  21682	  1.02%
 44	  15850	  0.74%
 45	  20130	  0.95%
 46	  15151	  0.71%
 47	  11623	  0.55%
 48	   1068	  0.05%
 49	   2190	  0.10%
 50	  90379	  4.25%


criterion=sequence-density
sequence-density=2.75
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCT


criterion=fanout-score
sequence-density=0.50
sequence-density-rank=29
fanout-score=16.77
fanout-score-rank=1
prefix-density=8.32
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 07:05:53
                             Started mapping on |	Mar 04 07:05:53
                                    Finished on |	Mar 04 07:06:21
       Mapping speed, Million of reads per hour |	280.80

                          Number of input reads |	2183963
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1172566
                        Uniquely mapped reads % |	53.69%
                          Average mapped length |	24.90
                       Number of splices: Total |	1313
            Number of splices: Annotated (sjdb) |	299
                       Number of splices: GT/AG |	1248
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	51
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	712778
             % of reads mapped to multiple loci |	32.64%
        Number of reads mapped to too many loci |	62805
             % of reads mapped to too many loci |	2.88%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.65%
                     % of reads unmapped: other |	1.14%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	298619	298619	298619
N_multimapping	712778	712778	712778
N_noFeature	80076	94855	784117
N_ambiguous	630006	284516	9550
UnstrandedReadsAssigned:462484 PositiveStrandReadsAssigned:793195 NegativeStrandReadsAssigned:378899
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031006 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031006-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,183,963 reads, 550,916 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,154 rounds

 109283 SRR5031006.ke.tsv
  52636 SRR5031006.se.tsv
 161919 total
==> SRR5031006.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031006.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031006 completed mapping pipeline successfully
