Starting /dee2/code/volunteer_pipeline.sh SRR5031024
    current disk space = 160534839296
    free memory = 1048502788 
SRR5031024 SRAfilesize
8685f84d84f73a6e7f707b90f31a858e  SRR5031024.sra
SRR5031024.sra file validated
SRR5031024 is single end
SRR5031024 is conventional basespace
SRR5031024 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031024_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41175	33.0	31.0	34.0	31.0	34.0
2	32.583	34.0	31.0	34.0	31.0	34.0
3	32.63875	34.0	31.0	34.0	31.0	34.0
4	36.197	37.0	37.0	37.0	35.0	37.0
5	36.144	37.0	35.0	37.0	35.0	37.0
6	35.952	37.0	35.0	37.0	35.0	37.0
7	35.89325	37.0	35.0	37.0	35.0	37.0
8	36.02575	37.0	35.0	37.0	35.0	37.0
9	37.678	39.0	37.0	39.0	35.0	39.0
10	37.65925	39.0	37.0	39.0	35.0	39.0
11	37.45675	39.0	37.0	39.0	34.0	39.0
12	37.74375	39.0	37.0	39.0	35.0	39.0
13	37.6895	39.0	37.0	39.0	35.0	39.0
14	39.1055	40.0	38.0	41.0	36.0	41.0
15	38.9215	40.0	38.0	41.0	35.0	41.0
16	38.97275	40.0	38.0	41.0	35.0	41.0
17	39.146	40.0	38.0	41.0	36.0	41.0
18	39.14275	41.0	38.0	41.0	36.0	41.0
19	38.9625	40.0	38.0	41.0	35.0	41.0
20	38.9055	40.0	39.0	41.0	35.0	41.0
21	38.89275	40.0	38.0	41.0	34.0	41.0
22	39.0335	40.0	39.0	41.0	36.0	41.0
23	38.6275	40.0	38.0	41.0	34.0	41.0
24	38.968	40.0	39.0	41.0	35.0	41.0
25	39.078	41.0	39.0	41.0	35.0	41.0
26	38.9735	40.0	39.0	41.0	35.0	41.0
27	39.0505	41.0	39.0	41.0	35.0	41.0
28	38.87225	40.0	38.0	41.0	35.0	41.0
29	38.6595	40.0	38.0	41.0	34.0	41.0
30	38.63375	40.0	38.0	41.0	34.0	41.0
31	38.797	40.0	38.0	41.0	35.0	41.0
32	38.811	40.0	38.0	41.0	35.0	41.0
33	38.5145	40.0	38.0	41.0	34.0	41.0
34	38.44225	40.0	38.0	41.0	34.0	41.0
35	38.14925	40.0	38.0	41.0	34.0	41.0
36	38.1145	40.0	38.0	41.0	33.0	41.0
37	37.5505	40.0	37.0	41.0	32.0	41.0
38	37.50475	40.0	37.0	41.0	32.0	41.0
39	37.8405	40.0	37.0	41.0	33.0	41.0
40	38.01925	40.0	38.0	41.0	33.0	41.0
41	38.08825	40.0	38.0	41.0	33.0	41.0
42	38.09425	40.0	38.0	41.0	33.0	41.0
43	37.989	40.0	38.0	41.0	33.0	41.0
44	38.16325	40.0	38.0	41.0	33.0	41.0
45	38.06225	40.0	38.0	41.0	33.0	41.0
46	37.94	40.0	37.0	41.0	33.0	41.0
47	37.52475	40.0	37.0	41.0	32.0	41.0
48	37.38225	40.0	37.0	41.0	32.0	41.0
49	37.4385	40.0	37.0	41.0	32.0	41.0
50	36.83925	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	1.0
18	3.0
19	0.0
20	2.0
21	1.0
22	4.0
23	2.0
24	2.0
25	11.0
26	23.0
27	13.0
28	28.0
29	27.0
30	31.0
31	60.0
32	68.0
33	95.0
34	151.0
35	183.0
36	268.0
37	442.0
38	710.0
39	1872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.375	24.825	54.775	13.025
2	23.87760220717331	28.016052169551042	19.588663155254576	28.517682468021064
3	23.05	23.799999999999997	23.275000000000002	29.875
4	32.65	29.099999999999998	14.325	23.925
5	22.875	39.725	27.825	9.575
6	34.849999999999994	16.375	34.075	14.7
7	15.775	22.425	23.45	38.35
8	26.674999999999997	29.075	15.925	28.325
9	18.45	36.475	29.575000000000003	15.5
10	20.724999999999998	25.1	27.05	27.125
11	32.775	7.875	18.025	41.325
12	13.825000000000001	20.7	30.675	34.8
13	31.65	17.474999999999998	32.7	18.175
14	34.599999999999994	28.549999999999997	22.85	14.000000000000002
15	37.425000000000004	21.15	27.275	14.149999999999999
16	19.7	17.325	24.85	38.125
17	24.65	24.025	39.0	12.325
18	27.275	17.549999999999997	26.900000000000002	28.275
19	33.375	14.475	34.625	17.525
20	42.975	15.049999999999999	28.449999999999996	13.525
21	21.6	19.05	31.374999999999996	27.975
22	30.3	11.200000000000001	48.775	9.725
23	27.925	12.425	46.575	13.075000000000001
24	53.65	13.675	25.575	7.1
25	52.775000000000006	24.975	14.325	7.925
26	28.050000000000004	48.75	14.075	9.125
27	14.649999999999999	49.45	25.05	10.85
28	10.575	23.3	51.824999999999996	14.299999999999999
29	11.25	11.25	58.099999999999994	19.400000000000002
30	8.9	5.7250000000000005	38.074999999999996	47.3
31	14.399999999999999	4.725	46.300000000000004	34.575
32	29.349999999999998	4.9750000000000005	20.674999999999997	45.0
33	56.99999999999999	6.45	13.075000000000001	23.474999999999998
34	65.45	6.25	14.875	13.425
35	66.35	7.000000000000001	18.775	7.875
36	42.375	6.65	38.125	12.85
37	45.85	9.525	20.025000000000002	24.6
38	22.675	15.125	11.924999999999999	50.275000000000006
39	14.124999999999998	24.9	9.175	51.800000000000004
40	15.174999999999999	50.925	9.125	24.775
41	25.825	51.2	7.55	15.425
42	51.225	30.55	9.15	9.075
43	53.27499999999999	30.375000000000004	6.525	9.825000000000001
44	29.625	50.8	6.6000000000000005	12.975
45	15.725	51.775000000000006	13.100000000000001	19.400000000000002
46	10.725	25.5	19.0	44.775
47	7.2749999999999995	14.224999999999998	39.7	38.800000000000004
48	8.200000000000001	12.85	20.549999999999997	58.4
49	11.425	20.474999999999998	14.299999999999999	53.800000000000004
50	17.95	39.300000000000004	13.750000000000002	28.999999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	0.5
30	0.0
31	4.0
32	8.0
33	8.0
34	8.0
35	10.0
36	12.0
37	15.0
38	18.0
39	22.0
40	26.0
41	30.0
42	34.0
43	103.5
44	173.0
45	224.0
46	275.0
47	388.0
48	501.0
49	638.5
50	776.0
51	838.0
52	900.0
53	646.0
54	392.0
55	387.5
56	383.0
57	292.0
58	201.0
59	119.0
60	37.0
61	35.5
62	34.0
63	57.0
64	80.0
65	47.0
66	14.0
67	14.0
68	14.0
69	26.5
70	39.0
71	51.0
72	63.0
73	34.5
74	6.0
75	3.5
76	1.0
77	1.0
78	1.0
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.325
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14884696016772	27.6
2	8.665269042627532	6.2
3	3.6338225017470296	3.9
4	2.166317260656883	3.1
5	1.4675052410901468	2.625
6	1.0482180293501049	2.25
7	0.6988120195667366	1.7500000000000002
8	0.6988120195667366	2.0
9	0.4891684136967156	1.575
>10	3.2145352900069883	21.7
>50	0.4891684136967156	13.5
>100	0.2795248078266946	13.8
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	168	4.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	145	3.6249999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	135	3.375	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	104	2.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	96	2.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	87	2.175	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	86	2.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	55	1.375	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	52	1.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	32	0.8	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	30	0.75	RNA PCR Primer, Index 7 (97% over 35bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	28	0.7000000000000001	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	20	0.5	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 44 (100% over 50bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	11	0.27499999999999997	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	11	0.27499999999999997	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTC	9	0.22499999999999998	RNA PCR Primer, Index 44 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	8	0.2	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	8	0.2	No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	8	0.2	RNA PCR Primer, Index 3 (100% over 34bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	6	0.15	RNA PCR Primer, Index 3 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	6	0.15	RNA PCR Primer, Index 1 (100% over 33bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	5	0.125	No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGGAATTCTCGGGT	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.55	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.625	0.0	0.0	0.0
7	0.0	0.9	0.0	0.0	0.0
8	0.0	1.05	0.0	0.0	0.0
9	0.0	1.2	0.0	0.0	0.0
10	0.0	1.45	0.0	0.0	0.0
11	0.0	1.625	0.0	0.0	0.0
12	0.0	1.825	0.0	0.0	0.0
13	0.0	2.075	0.0	0.0	0.0
14	0.0	2.45	0.0	0.0	0.0
15	0.0	3.0	0.0	0.0	0.0
16	0.0	4.25	0.0	0.0	0.0
17	0.0	5.075	0.0	0.0	0.0
18	0.0	6.325	0.0	0.0	0.0
19	0.0	6.75	0.0	0.0	0.0
20	0.0	8.025	0.0	0.0	0.0
21	0.0	14.125	0.0	0.0	0.0
22	0.0	27.075	0.0	0.0	0.0
23	0.0	58.775	0.0	0.0	0.0
24	0.0	72.55	0.0	0.0	0.0
25	0.0	78.525	0.0	0.0	0.0
26	0.0	79.8	0.0	0.0	0.0
27	0.0	80.525	0.0	0.0	0.0
28	0.0	81.15	0.0	0.0	0.0
29	0.0	81.65	0.0	0.0	0.0
30	0.0	82.125	0.0	0.0	0.0
31	0.0	82.8	0.0	0.0	0.0
32	0.0	83.825	0.0	0.0	0.0
33	0.0	84.75	0.0	0.0	0.0
34	0.0	87.1	0.0	0.0	0.0
35	0.0	87.775	0.0	0.0	0.0
36	0.0	88.375	0.0	0.0	0.0
37	0.0	89.0	0.0	0.0	0.0
38	0.0	89.425	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACT	20	6.952051E-4	44.000004	13
ACAGTGA	40	5.315087E-9	44.000004	3
ACCGGTC	40	5.315087E-9	44.000004	10
GGTGAGT	40	5.315087E-9	44.000004	17
ACTCGAC	20	6.952051E-4	44.000004	12
AACCGGT	40	5.315087E-9	44.000004	9
TCGACTC	20	6.952051E-4	44.000004	14
ACCATCA	20	6.952051E-4	44.000004	6
GGTCTCT	40	5.315087E-9	44.000004	13
CATCACT	20	6.952051E-4	44.000004	8
ACATTCA	65	0.0	44.000004	2
AACCATC	20	6.952051E-4	44.000004	5
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGTCG	65	0.0	44.000004	11
GAACCGG	40	5.315087E-9	44.000004	8
GAACCAT	20	6.952051E-4	44.000004	4
GTCTCTT	40	5.315087E-9	44.000004	14
CCATCAC	20	6.952051E-4	44.000004	7
CGGTGAG	40	5.315087E-9	44.000004	16
CACTCGA	20	6.952051E-4	44.000004	11
>>END_MODULE
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
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Read 61058 spots for SRR5031024.sra
Written 61058 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
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Written 61016 spots for SRR5031024.sra
Read 61016 spots for SRR5031024.sra
Written 61016 spots for SRR5031024.sra
SRR ids: ['SRR5031024.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8nj8dknl
SRR5031024.sra spots: 2928810
blocks: [[1, 61016], [61017, 122032], [122033, 183048], [183049, 244064], [244065, 305080], [305081, 366096], [366097, 427112], [427113, 488128], [488129, 549144], [549145, 610160], [610161, 671176], [671177, 732192], [732193, 793208], [793209, 854224], [854225, 915240], [915241, 976256], [976257, 1037272], [1037273, 1098288], [1098289, 1159304], [1159305, 1220320], [1220321, 1281336], [1281337, 1342352], [1342353, 1403368], [1403369, 1464384], [1464385, 1525400], [1525401, 1586416], [1586417, 1647432], [1647433, 1708448], [1708449, 1769464], [1769465, 1830480], [1830481, 1891496], [1891497, 1952512], [1952513, 2013528], [2013529, 2074544], [2074545, 2135560], [2135561, 2196576], [2196577, 2257592], [2257593, 2318608], [2318609, 2379624], [2379625, 2440640], [2440641, 2501656], [2501657, 2562672], [2562673, 2623688], [2623689, 2684704], [2684705, 2745720], [2745721, 2806736], [2806737, 2867752], [2867753, 2928810]]
SRR5031024 file size 506561
SRR5031024 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR5031024 SRR5031024_1.fastq
Input file:	SRR5031024_1.fastq
trimmed:	SRR5031024-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Wed Mar  4 07:08:52 2020 >> started

Wed Mar  4 07:08:54 2020 >> done (1.845s)
2928810 reads processed; of these:
    196 ( 0.01%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
2928611 (99.99%) reads available; of these:
 155746 ( 5.32%) trimmed reads available after processing
2772865 (94.68%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     55	  0.00%
 19	     67	  0.00%
 20	    171	  0.01%
 21	    189	  0.01%
 22	    347	  0.01%
 23	    395	  0.01%
 24	    401	  0.01%
 25	    548	  0.02%
 26	    430	  0.01%
 27	    605	  0.02%
 28	    706	  0.02%
 29	   1211	  0.04%
 30	    708	  0.02%
 31	    823	  0.03%
 32	    928	  0.03%
 33	   1168	  0.04%
 34	   1440	  0.05%
 35	   2789	  0.10%
 36	   2441	  0.08%
 37	   2671	  0.09%
 38	   2626	  0.09%
 39	   2792	  0.10%
 40	   3039	  0.10%
 41	   3325	  0.11%
 42	   4311	  0.15%
 43	   6179	  0.21%
 44	   8572	  0.29%
 45	  10502	  0.36%
 46	  14757	  0.50%
 47	  18259	  0.62%
 48	  29411	  1.00%
 49	  33880	  1.16%
 50	2772865	 94.68%
2928611 reads passed initial QC


criterion=sequence-density
sequence-density=89.24
sequence-density-rank=1
fanout-score=21.03
fanout-score-rank=1
prefix-density=90.52
prefix-fanout=20.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC


criterion=fanout-score
sequence-density=89.24
sequence-density-rank=1
fanout-score=21.03
fanout-score-rank=1
prefix-density=90.52
prefix-fanout=20.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC -o SRR5031024 -
Input file:	STDIN
trimmed:	SRR5031024-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Wed Mar  4 07:09:11 2020 >> started

Wed Mar  4 07:09:15 2020 >> done (4.349s)
2863531 reads processed; of these:
 171289 ( 5.98%) short reads filtered out after trimming by size control
  15896 ( 0.56%) empty reads filtered out after trimming by size control
2676346 (93.46%) reads available; of these:
2573225 (96.15%) trimmed reads available after processing
 103121 ( 3.85%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  14342	  0.54%
 19	  35610	  1.33%
 20	 166861	  6.23%
 21	 393683	 14.71%
 22	 902007	 33.70%
 23	 400992	 14.98%
 24	 177503	  6.63%
 25	  40250	  1.50%
 26	  21258	  0.79%
 27	  17794	  0.66%
 28	  17942	  0.67%
 29	  14121	  0.53%
 30	  20980	  0.78%
 31	  24914	  0.93%
 32	  30688	  1.15%
 33	  67465	  2.52%
 34	  24399	  0.91%
 35	  18447	  0.69%
 36	  20353	  0.76%
 37	  11068	  0.41%
 38	  12444	  0.46%
 39	  11180	  0.42%
 40	  16221	  0.61%
 41	  22577	  0.84%
 42	  27590	  1.03%
 43	  19027	  0.71%
 44	  12956	  0.48%
 45	  19153	  0.72%
 46	  14201	  0.53%
 47	  10756	  0.40%
 48	    987	  0.04%
 49	   1548	  0.06%
 50	  87029	  3.25%


criterion=sequence-density
sequence-density=7.75
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=37
fanout-score=144.83
fanout-score-rank=1
prefix-density=3.74
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 07:09:56
                             Started mapping on |	Mar 04 07:09:57
                                    Finished on |	Mar 04 07:10:27
       Mapping speed, Million of reads per hour |	328.97

                          Number of input reads |	2741426
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1408080
                        Uniquely mapped reads % |	51.36%
                          Average mapped length |	23.72
                       Number of splices: Total |	1472
            Number of splices: Annotated (sjdb) |	365
                       Number of splices: GT/AG |	1398
                       Number of splices: GC/AG |	12
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	60
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.83
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1020809
             % of reads mapped to multiple loci |	37.24%
        Number of reads mapped to too many loci |	47304
             % of reads mapped to too many loci |	1.73%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.16%
                     % of reads unmapped: other |	0.52%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	312537	312537	312537
N_multimapping	1020809	1020809	1020809
N_noFeature	61128	74445	983674
N_ambiguous	783345	411599	10559
UnstrandedReadsAssigned:563607 PositiveStrandReadsAssigned:922036 NegativeStrandReadsAssigned:413847
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031024 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031024-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,741,426 reads, 679,685 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,089 rounds

 109283 SRR5031024.ke.tsv
  52636 SRR5031024.se.tsv
 161919 total
==> SRR5031024.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031024.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031024 completed mapping pipeline successfully
