Starting /dee2/code/volunteer_pipeline.sh SRR5030939
    current disk space = 160611614720
    free memory = 73946568 
SRR5030939 SRAfilesize
53f7d23e4a2166c55a6233c25845a63a  SRR5030939.sra
SRR5030939.sra file validated
SRR5030939 is single end
SRR5030939 is conventional basespace
SRR5030939 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45225	33.0	31.0	34.0	31.0	34.0
2	32.745	34.0	31.0	34.0	31.0	34.0
3	32.75275	34.0	31.0	34.0	31.0	34.0
4	36.21225	37.0	37.0	37.0	35.0	37.0
5	36.30875	37.0	37.0	37.0	35.0	37.0
6	36.10175	37.0	36.0	37.0	35.0	37.0
7	36.13225	37.0	35.0	37.0	35.0	37.0
8	36.15275	37.0	36.0	37.0	35.0	37.0
9	37.9645	39.0	38.0	39.0	35.0	39.0
10	37.936	39.0	38.0	39.0	35.0	39.0
11	37.773	39.0	38.0	39.0	35.0	39.0
12	37.77525	39.0	38.0	39.0	35.0	39.0
13	37.70075	39.0	38.0	39.0	35.0	39.0
14	39.1535	40.0	39.0	41.0	36.0	41.0
15	39.228	41.0	39.0	41.0	36.0	41.0
16	39.197	40.0	38.0	41.0	36.0	41.0
17	39.18925	40.0	39.0	41.0	36.0	41.0
18	39.3745	41.0	39.0	41.0	36.0	41.0
19	39.2475	41.0	39.0	41.0	36.0	41.0
20	39.21375	40.0	39.0	41.0	36.0	41.0
21	39.21775	40.0	39.0	41.0	36.0	41.0
22	39.32125	41.0	39.0	41.0	36.0	41.0
23	39.28425	41.0	39.0	41.0	36.0	41.0
24	38.54675	40.0	38.0	41.0	34.0	41.0
25	38.71575	40.0	38.0	41.0	35.0	41.0
26	38.9745	40.0	39.0	41.0	35.0	41.0
27	39.07875	41.0	39.0	41.0	35.0	41.0
28	38.67	40.0	38.0	41.0	34.0	41.0
29	38.01925	40.0	38.0	41.0	32.0	41.0
30	38.14375	40.0	38.0	41.0	33.0	41.0
31	38.64425	40.0	38.0	41.0	34.0	41.0
32	38.68875	40.0	38.0	41.0	35.0	41.0
33	37.78525	40.0	37.0	41.0	32.0	41.0
34	37.45825	40.0	37.0	41.0	31.0	41.0
35	37.3405	39.0	37.0	40.0	31.0	41.0
36	33.4305	38.0	30.0	40.0	18.0	41.0
37	35.8175	38.0	34.0	40.0	30.0	41.0
38	37.228	39.0	37.0	40.0	32.0	41.0
39	37.38675	39.0	37.0	40.0	32.0	41.0
40	37.40975	39.0	37.0	41.0	33.0	41.0
41	37.11175	39.0	36.0	40.0	32.0	41.0
42	37.0395	39.0	36.0	40.0	32.0	41.0
43	37.11075	39.0	36.0	41.0	32.0	41.0
44	37.3185	39.0	37.0	41.0	33.0	41.0
45	36.80425	39.0	36.0	41.0	31.0	41.0
46	34.603	38.0	33.0	40.0	25.0	41.0
47	35.388	38.0	34.0	40.0	28.0	41.0
48	35.35425	38.0	34.0	40.0	28.0	41.0
49	34.78225	38.0	34.0	40.0	27.0	41.0
50	30.3795	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	2.0
16	0.0
17	2.0
18	2.0
19	1.0
20	5.0
21	5.0
22	3.0
23	6.0
24	8.0
25	12.0
26	17.0
27	27.0
28	33.0
29	38.0
30	44.0
31	65.0
32	116.0
33	124.0
34	170.0
35	211.0
36	320.0
37	518.0
38	1047.0
39	1221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.643965948923386	25.28793189784677	46.194291437155734	15.87381071607411
2	22.75	25.775	21.0	30.475
3	27.224999999999998	16.75	22.125	33.900000000000006
4	32.65	29.225	14.6	23.525
5	20.175	40.1	25.6	14.124999999999998
6	28.349999999999998	18.5	39.825	13.325000000000001
7	20.875	19.35	21.75	38.025
8	21.925	30.0	16.45	31.624999999999996
9	23.400000000000002	31.775	27.925	16.900000000000002
10	24.3	17.5	31.924999999999997	26.275
11	33.025	17.0	14.475	35.5
12	15.85	22.95	31.1	30.099999999999998
13	28.1	19.325	30.325000000000003	22.25
14	26.650000000000002	32.25	22.0	19.1
15	34.075	24.099999999999998	25.775	16.05
16	18.45	22.225	21.8	37.525
17	25.15	21.7	36.95	16.2
18	23.849999999999998	18.025	34.275	23.849999999999998
19	35.825	15.125	31.724999999999998	17.325
20	41.699999999999996	17.825	24.7	15.775
21	24.099999999999998	21.325	24.975	29.599999999999998
22	29.599999999999998	16.225	42.6	11.575000000000001
23	27.1	13.475000000000001	41.3	18.125
24	47.825	15.625	24.9	11.65
25	46.175	23.9	15.2	14.725
26	27.3	42.75	15.950000000000001	14.000000000000002
27	18.0	42.85	23.525	15.625
28	15.0	23.724999999999998	43.7	17.575
29	12.025	15.475	52.75	19.75
30	11.85	9.5	32.975	45.675
31	16.8	7.199999999999999	44.15	31.85
32	28.499999999999996	8.7	22.125	40.675
33	51.2	11.275	14.725	22.8
34	54.85	9.8	18.3	17.05
35	58.225	9.525	18.525	13.725000000000001
36	41.725	11.425	32.025	14.825
37	43.375	11.799999999999999	20.8	24.025
38	24.0	16.650000000000002	14.424999999999999	44.925
39	17.2	24.9	11.899999999999999	46.0
40	18.55	43.55	15.1	22.8
41	25.224999999999998	43.5	13.05	18.224999999999998
42	47.8	27.675	13.8	10.725
43	47.375	30.225	11.55	10.85
44	30.0	45.35	9.700000000000001	14.95
45	19.025	48.199999999999996	15.375	17.4
46	15.625	26.700000000000003	19.6	38.074999999999996
47	11.85	17.0	37.4	33.75
48	12.65	14.75	20.200000000000003	52.400000000000006
49	16.025	20.3	17.025000000000002	46.650000000000006
50	20.05	34.25	15.475	30.225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	0.5
26	0.0
27	2.0
28	4.0
29	2.5
30	1.0
31	11.0
32	21.0
33	22.5
34	24.0
35	45.0
36	66.0
37	74.5
38	83.0
39	79.0
40	75.0
41	70.0
42	65.0
43	106.5
44	148.0
45	170.5
46	193.0
47	289.5
48	386.0
49	473.0
50	560.0
51	648.0
52	736.0
53	570.5
54	405.0
55	465.0
56	525.0
57	404.0
58	283.0
59	192.0
60	101.0
61	80.5
62	60.0
63	88.0
64	116.0
65	79.0
66	42.0
67	24.0
68	6.0
69	16.0
70	26.0
71	37.5
72	49.0
73	29.5
74	10.0
75	6.0
76	2.0
77	2.0
78	2.0
79	3.0
80	4.0
81	4.5
82	5.0
83	2.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.11696264543784	29.849999999999998
2	11.941212492345377	9.75
3	4.102878138395591	5.025
4	1.653398652786283	2.7
5	1.5921616656460504	3.25
6	1.653398652786283	4.05
7	0.8573178199632578	2.45
8	0.6736068585425597	2.1999999999999997
9	0.3674219228413962	1.35
>10	3.551745254133497	23.674999999999997
>50	0.3674219228413962	10.2
>100	0.1224739742804654	5.5
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	117	2.9250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	87	2.175	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	50	1.25	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	29	0.7250000000000001	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	26	0.65	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	24	0.6	RNA PCR Primer, Index 5 (97% over 35bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	21	0.525	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	19	0.475	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	18	0.44999999999999996	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	17	0.42500000000000004	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	16	0.4	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	16	0.4	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	15	0.375	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTC	14	0.35000000000000003	RNA PCR Primer, Index 48 (100% over 44bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	14	0.35000000000000003	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	14	0.35000000000000003	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	13	0.325	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC	13	0.325	RNA PCR Primer, Index 48 (100% over 50bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	13	0.325	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	11	0.27499999999999997	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	11	0.27499999999999997	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	11	0.27499999999999997	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	11	0.27499999999999997	No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	10	0.25	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	10	0.25	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	9	0.22499999999999998	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	8	0.2	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	8	0.2	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	7	0.17500000000000002	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	7	0.17500000000000002	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	7	0.17500000000000002	No Hit
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	6	0.15	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	6	0.15	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
AGCAGCATTGTACAGGGCTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	6	0.15	No Hit
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	5	0.125	RNA PCR Primer, Index 3 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CGCACGGGGTCGGCGGCGATGTCGGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCAATTAGTAGATTCTGAATAAACCCAGAAGAGAGTACCATGGAATTC	5	0.125	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTG	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	5	0.125	No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.35	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.45	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.55	0.0	0.0	0.0
7	0.0	1.05	0.0	0.0	0.0
8	0.0	1.275	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.475	0.0	0.0	0.0
11	0.0	1.65	0.0	0.0	0.0
12	0.0	1.975	0.0	0.0	0.0
13	0.0	2.125	0.0	0.0	0.0
14	0.0	2.375	0.0	0.0	0.0
15	0.0	2.675	0.0	0.0	0.0
16	0.0	3.75	0.0	0.0	0.0
17	0.0	4.2	0.0	0.0	0.0
18	0.0	5.575	0.0	0.0	0.0
19	0.0	6.1	0.0	0.0	0.0
20	0.0	6.7	0.0	0.0	0.0
21	0.0	12.075	0.0	0.0	0.0
22	0.0	21.65	0.0	0.0	0.0
23	0.0	46.775	0.0	0.0	0.0
24	0.0	57.275	0.0	0.0	0.0
25	0.0	62.475	0.0	0.0	0.0
26	0.0	64.075	0.0	0.0	0.0
27	0.0	64.725	0.0	0.0	0.0
28	0.0	65.1	0.0	0.0	0.0
29	0.0	65.525	0.0	0.0	0.0
30	0.0	66.0	0.0	0.0	0.0
31	0.0	66.8	0.0	0.0	0.0
32	0.0	68.3	0.0	0.0	0.0
33	0.0	69.05	0.0	0.0	0.0
34	0.0	71.325	0.0	0.0	0.0
35	0.0	72.3	0.0	0.0	0.0
36	0.0	73.35	0.0	0.0	0.0
37	0.0	74.125	0.0	0.0	0.0
38	0.0	74.65	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
GTAGATT	20	6.952051E-4	44.000004	8
CTGAGAC	20	6.952051E-4	44.000004	4
AGTAGAT	20	6.952051E-4	44.000004	7
CCTGAGA	20	6.952051E-4	44.000004	3
GGTTTGG	20	6.952051E-4	44.000004	19
GATTGTA	20	6.952051E-4	44.000004	11
CTTGGCT	20	6.952051E-4	44.000004	16
TAGTAGA	20	6.952051E-4	44.000004	6
ATTGTAT	20	6.952051E-4	44.000004	12
CCCTGAG	20	6.952051E-4	44.000004	2
GGTAGTA	45	2.728484E-10	44.000004	4
GCTTGGC	20	6.952051E-4	44.000004	15
TGAGGTA	45	2.728484E-10	44.000004	1
AGGTAGT	45	2.728484E-10	44.000004	3
AGACCCT	20	6.952051E-4	44.000004	7
AGATTGT	20	6.952051E-4	44.000004	10
TAGATTG	20	6.952051E-4	44.000004	9
GTAGTAG	45	2.728484E-10	44.000004	5
AACGCTG	25	3.6958358E-5	44.0	8
>>END_MODULE
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181877 spots for SRR5030939.sra
Written 181877 spots for SRR5030939.sra
Read 181880 spots for SRR5030939.sra
Written 181880 spots for SRR5030939.sra
SRR ids: ['SRR5030939.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_sdval66i
SRR5030939.sra spots: 2182527
blocks: [[1, 181877], [181878, 363754], [363755, 545631], [545632, 727508], [727509, 909385], [909386, 1091262], [1091263, 1273139], [1273140, 1455016], [1455017, 1636893], [1636894, 1818770], [1818771, 2000647], [2000648, 2182527]]
SRR5030939 file size 377199
SRR5030939 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030939 SRR5030939_1.fastq
Input file:	SRR5030939_1.fastq
trimmed:	SRR5030939-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:49:42 2020 >> started

Wed Mar  4 06:49:44 2020 >> done (1.443s)
2182527 reads processed; of these:
    180 ( 0.01%) short reads filtered out after trimming by size control
     11 ( 0.00%) empty reads filtered out after trimming by size control
2182336 (99.99%) reads available; of these:
 173690 ( 7.96%) trimmed reads available after processing
2008646 (92.04%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     37	  0.00%
 19	     47	  0.00%
 20	    133	  0.01%
 21	    117	  0.01%
 22	    225	  0.01%
 23	    257	  0.01%
 24	    299	  0.01%
 25	    359	  0.02%
 26	    340	  0.02%
 27	    429	  0.02%
 28	    481	  0.02%
 29	    709	  0.03%
 30	    628	  0.03%
 31	    773	  0.04%
 32	   1038	  0.05%
 33	   1862	  0.09%
 34	   5103	  0.23%
 35	   2544	  0.12%
 36	   1772	  0.08%
 37	   1984	  0.09%
 38	   2866	  0.13%
 39	   2812	  0.13%
 40	   4515	  0.21%
 41	   3266	  0.15%
 42	   6065	  0.28%
 43	   4619	  0.21%
 44	  11701	  0.54%
 45	  12065	  0.55%
 46	  11914	  0.55%
 47	  14075	  0.64%
 48	  31918	  1.46%
 49	  48737	  2.23%
 50	2008646	 92.04%
2182336 reads passed initial QC


criterion=sequence-density
sequence-density=74.06
sequence-density-rank=1
fanout-score=21.44
fanout-score-rank=2
prefix-density=76.82
prefix-fanout=20.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC


criterion=fanout-score
sequence-density=2.05
sequence-density-rank=6
fanout-score=118.72
fanout-score-rank=1
prefix-density=75.90
prefix-fanout=3.2
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC -o SRR5030939 -
Input file:	STDIN
trimmed:	SRR5030939-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:49:59 2020 >> started

Wed Mar  4 06:50:02 2020 >> done (2.843s)
2124140 reads processed; of these:
 106553 ( 5.02%) short reads filtered out after trimming by size control
   7954 ( 0.37%) empty reads filtered out after trimming by size control
2009633 (94.61%) reads available; of these:
1702300 (84.71%) trimmed reads available after processing
 307333 (15.29%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  11697	  0.58%
 19	  16000	  0.80%
 20	 108044	  5.38%
 21	 208759	 10.39%
 22	 536559	 26.70%
 23	 234533	 11.67%
 24	 111581	  5.55%
 25	  32056	  1.60%
 26	  15608	  0.78%
 27	  12143	  0.60%
 28	  13414	  0.67%
 29	  10238	  0.51%
 30	  16158	  0.80%
 31	  31343	  1.56%
 32	  20605	  1.03%
 33	  55001	  2.74%
 34	  21528	  1.07%
 35	  18136	  0.90%
 36	  19404	  0.97%
 37	  12458	  0.62%
 38	  13940	  0.69%
 39	  14365	  0.71%
 40	  22616	  1.13%
 41	  27776	  1.38%
 42	  32656	  1.62%
 43	  23772	  1.18%
 44	  19253	  0.96%
 45	  27046	  1.35%
 46	  21026	  1.05%
 47	  16692	  0.83%
 48	   3511	  0.17%
 49	  10404	  0.52%
 50	 271311	 13.50%


criterion=sequence-density
sequence-density=6.28
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.76
sequence-density-rank=18
fanout-score=11.48
fanout-score-rank=1
prefix-density=8.74
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:50:27
                             Started mapping on |	Mar 04 06:50:28
                                    Finished on |	Mar 04 06:50:54
       Mapping speed, Million of reads per hour |	286.31

                          Number of input reads |	2067829
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1224423
                        Uniquely mapped reads % |	59.21%
                          Average mapped length |	28.64
                       Number of splices: Total |	994
            Number of splices: Annotated (sjdb) |	182
                       Number of splices: GT/AG |	891
                       Number of splices: GC/AG |	14
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	86
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.46
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	553173
             % of reads mapped to multiple loci |	26.75%
        Number of reads mapped to too many loci |	56639
             % of reads mapped to too many loci |	2.74%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.41%
                     % of reads unmapped: other |	0.89%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	290233	290233	290233
N_multimapping	553173	553173	553173
N_noFeature	69619	87670	848044
N_ambiguous	671061	342087	14016
UnstrandedReadsAssigned:483743 PositiveStrandReadsAssigned:794666 NegativeStrandReadsAssigned:362363
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030939 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030939-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,067,829 reads, 540,224 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,082 rounds

 109283 SRR5030939.ke.tsv
  52636 SRR5030939.se.tsv
 161919 total
==> SRR5030939.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030939.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030939 completed mapping pipeline successfully
