Starting /dee2/code/volunteer_pipeline.sh SRR5030870
    current disk space = 160663060480
    free memory = 73956156 
SRR5030870 SRAfilesize
8f024bdaa1144398ef3ac3c8d11b3ed3  SRR5030870.sra
SRR5030870.sra file validated
SRR5030870 is single end
SRR5030870 is conventional basespace
SRR5030870 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30925	33.0	31.0	34.0	31.0	34.0
2	32.6335	34.0	31.0	34.0	31.0	34.0
3	32.72325	34.0	31.0	34.0	31.0	34.0
4	36.2265	37.0	37.0	37.0	35.0	37.0
5	36.25725	37.0	37.0	37.0	35.0	37.0
6	36.10275	37.0	35.0	37.0	35.0	37.0
7	36.05025	37.0	35.0	37.0	35.0	37.0
8	36.1145	37.0	36.0	37.0	35.0	37.0
9	37.8505	39.0	38.0	39.0	35.0	39.0
10	37.87025	39.0	38.0	39.0	35.0	39.0
11	37.82575	39.0	38.0	39.0	35.0	39.0
12	37.85175	39.0	38.0	39.0	35.0	39.0
13	37.78825	39.0	38.0	39.0	35.0	39.0
14	39.118	41.0	39.0	41.0	36.0	41.0
15	39.1615	40.0	39.0	41.0	36.0	41.0
16	39.1305	40.0	39.0	41.0	36.0	41.0
17	39.287	41.0	39.0	41.0	36.0	41.0
18	39.236	41.0	39.0	41.0	36.0	41.0
19	39.12225	40.0	39.0	41.0	36.0	41.0
20	39.0275	40.0	39.0	41.0	35.0	41.0
21	39.12825	40.0	39.0	41.0	35.0	41.0
22	39.0665	40.0	39.0	41.0	36.0	41.0
23	38.935	40.0	39.0	41.0	35.0	41.0
24	38.72575	40.0	38.0	41.0	34.0	41.0
25	38.9085	40.0	39.0	41.0	35.0	41.0
26	38.964	40.0	39.0	41.0	35.0	41.0
27	39.13975	41.0	39.0	41.0	36.0	41.0
28	38.8095	40.0	38.0	41.0	35.0	41.0
29	38.121	40.0	38.0	41.0	33.0	41.0
30	38.32875	40.0	38.0	41.0	34.0	41.0
31	38.459	40.0	38.0	41.0	34.0	41.0
32	38.704	40.0	38.0	41.0	35.0	41.0
33	37.55625	40.0	38.0	41.0	31.0	41.0
34	37.68025	40.0	37.0	41.0	32.0	41.0
35	37.27275	39.0	37.0	40.0	32.0	41.0
36	33.57825	38.0	30.0	40.0	18.0	41.0
37	35.12375	38.0	33.0	40.0	26.0	41.0
38	36.66925	38.0	36.0	40.0	31.0	41.0
39	36.78575	39.0	36.0	40.0	31.0	41.0
40	36.7935	39.0	36.0	40.0	31.0	41.0
41	36.76275	39.0	36.0	40.0	31.0	41.0
42	37.03275	40.0	37.0	41.0	31.0	41.0
43	36.7305	39.0	37.0	41.0	31.0	41.0
44	36.659	39.0	36.0	41.0	30.0	41.0
45	36.31375	39.0	36.0	40.0	30.0	41.0
46	34.62975	38.0	34.0	40.0	24.0	41.0
47	35.1195	38.0	34.0	40.0	27.0	41.0
48	35.22025	39.0	35.0	40.0	28.0	41.0
49	34.4745	38.0	34.0	40.0	26.0	41.0
50	28.7915	33.0	26.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	3.0
19	2.0
20	11.0
21	7.0
22	10.0
23	14.0
24	12.0
25	20.0
26	25.0
27	29.0
28	30.0
29	42.0
30	67.0
31	53.0
32	78.0
33	132.0
34	164.0
35	209.0
36	319.0
37	563.0
38	1097.0
39	1110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.701754385964913	23.358395989974937	49.373433583959894	16.56641604010025
2	23.575	24.625	19.35	32.45
3	25.5	18.475	20.175	35.85
4	32.375	26.224999999999998	14.85	26.55
5	20.05	42.325	23.875	13.750000000000002
6	32.6	15.725	35.825	15.85
7	17.375	21.55	25.1	35.975
8	26.3	28.575	16.5	28.625
9	23.849999999999998	32.7	28.9	14.549999999999999
10	23.025000000000002	20.925	31.574999999999996	24.474999999999998
11	29.2	14.674999999999999	16.5	39.625
12	15.024999999999999	20.1	31.8	33.074999999999996
13	30.275000000000002	19.675	30.475	19.575
14	26.35	33.475	21.375	18.8
15	34.883720930232556	25.581395348837212	23.605901475368842	15.928982245561391
16	19.2	21.575	21.875	37.35
17	23.0	21.725	40.2	15.075
18	22.2	19.2	33.5	25.1
19	40.675	13.875000000000002	27.950000000000003	17.5
20	41.675000000000004	15.2	29.675	13.450000000000001
21	24.925	18.425	26.775	29.875
22	27.6	13.525	46.85	12.025
23	26.1	13.225000000000001	43.85	16.825000000000003
24	50.275000000000006	15.65	24.025	10.05
25	48.025	25.374999999999996	15.1	11.5
26	27.056764191047762	44.26106526631658	14.20355088772193	14.47861965491373
27	17.150000000000002	45.95	23.150000000000002	13.750000000000002
28	13.225000000000001	23.875	45.7	17.2
29	13.100000000000001	13.675	55.225	18.0
30	10.2	8.375	33.45	47.975
31	14.95	7.5249999999999995	47.375	30.15
32	27.625	7.725	21.099999999999998	43.55
33	52.025	11.65	13.4	22.925
34	57.9	9.3	17.95	14.85
35	62.8	7.775	17.8	11.625
36	43.85	8.725	34.175	13.25
37	45.275	11.825	19.575	23.325000000000003
38	22.825	17.825	12.3	47.05
39	16.325	23.525	11.725	48.425000000000004
40	16.950000000000003	45.6	14.224999999999998	23.225
41	24.349999999999998	46.800000000000004	11.3	17.549999999999997
42	49.275000000000006	28.299999999999997	12.975	9.45
43	48.8	28.875	10.625	11.700000000000001
44	29.775000000000002	47.675	8.25	14.299999999999999
45	20.349999999999998	49.975	12.625	17.05
46	14.649999999999999	27.200000000000003	17.849999999999998	40.300000000000004
47	10.6	16.325	40.849999999999994	32.225
48	13.325000000000001	13.475000000000001	19.575	53.625
49	15.625	18.375	14.85	51.15
50	19.85	34.475	13.900000000000002	31.775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.0
26	1.0
27	0.5
28	0.0
29	1.0
30	2.0
31	11.0
32	20.0
33	25.0
34	30.0
35	39.5
36	49.0
37	51.0
38	53.0
39	54.0
40	55.0
41	60.0
42	65.0
43	88.0
44	111.0
45	153.5
46	196.0
47	306.5
48	417.0
49	487.5
50	558.0
51	666.5
52	775.0
53	596.0
54	417.0
55	473.0
56	529.0
57	425.5
58	322.0
59	209.0
60	96.0
61	70.5
62	45.0
63	98.0
64	151.0
65	101.0
66	51.0
67	34.5
68	18.0
69	14.0
70	10.0
71	11.0
72	12.0
73	7.5
74	3.0
75	5.0
76	7.0
77	4.0
78	1.0
79	1.5
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0788643533123	29.75
2	9.211356466876971	7.3
3	4.353312302839116	5.175
4	2.460567823343849	3.9
5	1.7665615141955835	3.5000000000000004
6	1.2618296529968454	3.0
7	1.0725552050473186	2.9749999999999996
8	0.7570977917981072	2.4
9	0.6309148264984227	2.25
>10	2.902208201892744	21.775
>50	0.31545741324921134	8.774999999999999
>100	0.1892744479495268	9.2
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	138	3.45	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	126	3.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	104	2.6	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	89	2.225	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	78	1.95	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	73	1.825	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	56	1.4000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	55	1.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	50	1.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	45	1.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	39	0.975	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC	33	0.8250000000000001	RNA PCR Primer, Index 20 (100% over 44bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	27	0.675	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	20	0.5	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	19	0.475	RNA PCR Primer, Index 1 (97% over 35bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGC	15	0.375	RNA PCR Primer, Index 20 (100% over 50bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	14	0.35000000000000003	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	13	0.325	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	12	0.3	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	12	0.3	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	11	0.27499999999999997	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	9	0.22499999999999998	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	9	0.22499999999999998	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	8	0.2	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGCC	8	0.2	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	8	0.2	No Hit
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	7	0.17500000000000002	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	6	0.15	RNA PCR Primer, Index 1 (96% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTAT	6	0.15	RNA PCR Primer, Index 20 (100% over 48bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
TGAGGTAGTAGGTTGTGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	5	0.125	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	5	0.125	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	5	0.125	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	5	0.125	No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	5	0.125	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACTGCTAATGAAAACACAG	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.4	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.575	0.0	0.0	0.0
4	0.0	0.6	0.0	0.0	0.0
5	0.0	0.625	0.0	0.0	0.0
6	0.0	0.7	0.0	0.0	0.0
7	0.0	1.675	0.0	0.0	0.0
8	0.0	1.725	0.0	0.0	0.0
9	0.0	1.9	0.0	0.0	0.0
10	0.0	2.025	0.0	0.0	0.0
11	0.0	2.3	0.0	0.0	0.0
12	0.0	2.675	0.0	0.0	0.0
13	0.0	2.825	0.0	0.0	0.0
14	0.0	3.175	0.0	0.0	0.0
15	0.0	3.525	0.0	0.0	0.0
16	0.0	4.575	0.0	0.0	0.0
17	0.0	5.2	0.0	0.0	0.0
18	0.0	6.125	0.0	0.0	0.0
19	0.0	6.625	0.0	0.0	0.0
20	0.0	7.325	0.0	0.0	0.0
21	0.0	12.35	0.0	0.0	0.0
22	0.0	22.225	0.0	0.0	0.0
23	0.0	50.825	0.0	0.0	0.0
24	0.0	62.125	0.0	0.0	0.0
25	0.0	67.6	0.0	0.0	0.0
26	0.0	69.0	0.0	0.0	0.0
27	0.0	69.825	0.0	0.0	0.0
28	0.0	70.5	0.0	0.0	0.0
29	0.0	70.975	0.0	0.0	0.0
30	0.0	71.325	0.0	0.0	0.0
31	0.0	72.075	0.0	0.0	0.0
32	0.0	72.825	0.0	0.0	0.0
33	0.0	73.475	0.0	0.0	0.0
34	0.0	77.375	0.0	0.0	0.0
35	0.0	78.275	0.0	0.0	0.0
36	0.0	79.15	0.0	0.0	0.0
37	0.0	80.075	0.0	0.0	0.0
38	0.0	80.35	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGGCT	20	6.952051E-4	44.000004	16
AGGCTTG	20	6.952051E-4	44.000004	18
GCTTGGC	20	6.952051E-4	44.000004	15
TTGGCTT	20	6.952051E-4	44.000004	17
AACGCTG	30	1.9515464E-6	44.0	8
GGTGAGT	25	3.6958358E-5	44.0	17
AAGTAAT	25	3.6958358E-5	44.0	4
TCAAGTA	25	3.6958358E-5	44.0	2
ACGCTGT	30	1.9515464E-6	44.0	9
AATCCAG	25	3.6958358E-5	44.0	8
ATCCAGG	25	3.6958358E-5	44.0	9
TTCAAGT	25	3.6958358E-5	44.0	1
TTCAACG	30	1.9515464E-6	44.0	5
AGGATAG	25	3.6958358E-5	44.0	13
ATAGGCT	25	3.6958358E-5	44.0	16
CATTCAA	30	1.9515464E-6	44.0	3
TCAACGC	30	1.9515464E-6	44.0	6
TCCAGGA	25	3.6958358E-5	44.0	10
ACATTCA	30	1.9515464E-6	44.0	2
GAGTTGG	25	3.6958358E-5	44.0	20
>>END_MODULE
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196984 spots for SRR5030870.sra
Written 196984 spots for SRR5030870.sra
Read 196988 spots for SRR5030870.sra
Written 196988 spots for SRR5030870.sra
SRR ids: ['SRR5030870.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5yjo33zh
SRR5030870.sra spots: 2363812
blocks: [[1, 196984], [196985, 393968], [393969, 590952], [590953, 787936], [787937, 984920], [984921, 1181904], [1181905, 1378888], [1378889, 1575872], [1575873, 1772856], [1772857, 1969840], [1969841, 2166824], [2166825, 2363812]]
SRR5030870 file size 408621
SRR5030870 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030870 SRR5030870_1.fastq
Input file:	SRR5030870_1.fastq
trimmed:	SRR5030870-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:32:46 2020 >> started

Wed Mar  4 06:32:47 2020 >> done (1.543s)
2363812 reads processed; of these:
    115 ( 0.00%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
2363692 (99.99%) reads available; of these:
 162022 ( 6.85%) trimmed reads available after processing
2201670 (93.15%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     46	  0.00%
 19	     43	  0.00%
 20	     85	  0.00%
 21	    100	  0.00%
 22	    185	  0.01%
 23	    286	  0.01%
 24	    317	  0.01%
 25	    391	  0.02%
 26	    332	  0.01%
 27	    422	  0.02%
 28	    583	  0.02%
 29	    782	  0.03%
 30	    654	  0.03%
 31	    826	  0.03%
 32	    866	  0.04%
 33	   1024	  0.04%
 34	   2417	  0.10%
 35	   1967	  0.08%
 36	   1491	  0.06%
 37	   1743	  0.07%
 38	   2086	  0.09%
 39	   2339	  0.10%
 40	   2561	  0.11%
 41	   2846	  0.12%
 42	   3816	  0.16%
 43	   5271	  0.22%
 44	   9410	  0.40%
 45	  11817	  0.50%
 46	  11434	  0.48%
 47	  15718	  0.66%
 48	  30466	  1.29%
 49	  49698	  2.10%
 50	2201670	 93.15%
2363692 reads passed initial QC


criterion=sequence-density
sequence-density=80.34
sequence-density-rank=1
fanout-score=18.50
fanout-score-rank=1
prefix-density=82.17
prefix-fanout=18.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC


criterion=fanout-score
sequence-density=80.34
sequence-density-rank=1
fanout-score=18.50
fanout-score-rank=1
prefix-density=82.17
prefix-fanout=18.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC -o SRR5030870 -
Input file:	STDIN
trimmed:	SRR5030870-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:33:01 2020 >> started

Wed Mar  4 06:33:04 2020 >> done (3.707s)
2305329 reads processed; of these:
 141854 ( 6.15%) short reads filtered out after trimming by size control
  12804 ( 0.56%) empty reads filtered out after trimming by size control
2150671 (93.29%) reads available; of these:
1892480 (87.99%) trimmed reads available after processing
 258191 (12.01%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12460	  0.58%
 19	  20361	  0.95%
 20	 120865	  5.62%
 21	 245757	 11.43%
 22	 654205	 30.42%
 23	 257368	 11.97%
 24	 120547	  5.61%
 25	  32984	  1.53%
 26	  16201	  0.75%
 27	  13044	  0.61%
 28	  14080	  0.65%
 29	  10115	  0.47%
 30	  15956	  0.74%
 31	  22128	  1.03%
 32	  19485	  0.91%
 33	  91270	  4.24%
 34	  25505	  1.19%
 35	  19505	  0.91%
 36	  22249	  1.03%
 37	  10857	  0.50%
 38	  11118	  0.52%
 39	  11763	  0.55%
 40	  16436	  0.76%
 41	  21605	  1.00%
 42	  25297	  1.18%
 43	  17641	  0.82%
 44	  15474	  0.72%
 45	  17937	  0.83%
 46	  13628	  0.63%
 47	  12592	  0.59%
 48	   3010	  0.14%
 49	  10507	  0.49%
 50	 228721	 10.63%


criterion=sequence-density
sequence-density=4.74
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCT


criterion=fanout-score
sequence-density=0.44
sequence-density-rank=29
fanout-score=27.35
fanout-score-rank=1
prefix-density=11.97
prefix-fanout=1.0
sequence=CTGAGCTTGGCA
                                 Started job on |	Mar 04 06:33:27
                             Started mapping on |	Mar 04 06:33:27
                                    Finished on |	Mar 04 06:33:51
       Mapping speed, Million of reads per hour |	331.36

                          Number of input reads |	2209034
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1280685
                        Uniquely mapped reads % |	57.97%
                          Average mapped length |	27.56
                       Number of splices: Total |	937
            Number of splices: Annotated (sjdb) |	214
                       Number of splices: GT/AG |	859
                       Number of splices: GC/AG |	16
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	62
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.50
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	645656
             % of reads mapped to multiple loci |	29.23%
        Number of reads mapped to too many loci |	53459
             % of reads mapped to too many loci |	2.42%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.85%
                     % of reads unmapped: other |	0.53%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	282693	282693	282693
N_multimapping	645656	645656	645656
N_noFeature	54415	67518	881285
N_ambiguous	741724	397426	12909
UnstrandedReadsAssigned:484546 PositiveStrandReadsAssigned:815741 NegativeStrandReadsAssigned:386491
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030870 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030870-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,209,034 reads, 617,321 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,143 rounds

 109283 SRR5030870.ke.tsv
  52636 SRR5030870.se.tsv
 161919 total
==> SRR5030870.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030870.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030870 completed mapping pipeline successfully
