Starting /dee2/code/volunteer_pipeline.sh SRR5030903
    current disk space = 469169160
    free memory = 131216112 
SRR5030903 SRAfilesize
01af44ca8066434d141dda876bcbf890  SRR5030903.sra
SRR5030903.sra file validated
SRR5030903 is single end
SRR5030903 is conventional basespace
SRR5030903 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50725	34.0	31.0	34.0	31.0	34.0
2	32.7695	34.0	31.0	34.0	31.0	34.0
3	32.882	34.0	31.0	34.0	31.0	34.0
4	36.2395	37.0	37.0	37.0	35.0	37.0
5	36.3645	37.0	37.0	37.0	35.0	37.0
6	36.197	37.0	37.0	37.0	35.0	37.0
7	36.083	37.0	35.0	37.0	35.0	37.0
8	36.2445	37.0	36.0	37.0	35.0	37.0
9	37.99875	39.0	38.0	39.0	35.0	39.0
10	38.07175	39.0	39.0	39.0	35.0	39.0
11	37.9645	39.0	38.0	39.0	35.0	39.0
12	37.9305	39.0	38.0	39.0	35.0	39.0
13	37.85475	39.0	38.0	39.0	35.0	39.0
14	39.365	41.0	39.0	41.0	36.0	41.0
15	39.3295	41.0	39.0	41.0	36.0	41.0
16	39.27475	40.0	39.0	41.0	36.0	41.0
17	39.4925	41.0	39.0	41.0	36.0	41.0
18	39.43775	41.0	39.0	41.0	36.0	41.0
19	39.25625	41.0	39.0	41.0	36.0	41.0
20	39.34775	41.0	39.0	41.0	36.0	41.0
21	39.40675	41.0	39.0	41.0	36.0	41.0
22	39.30525	41.0	39.0	41.0	36.0	41.0
23	39.13575	41.0	39.0	41.0	36.0	41.0
24	38.9895	40.0	39.0	41.0	35.0	41.0
25	39.234	41.0	39.0	41.0	36.0	41.0
26	39.302	41.0	39.0	41.0	36.0	41.0
27	39.357	41.0	39.0	41.0	37.0	41.0
28	39.20225	41.0	39.0	41.0	36.0	41.0
29	38.8505	41.0	39.0	41.0	35.0	41.0
30	39.02225	41.0	39.0	41.0	35.0	41.0
31	39.122	41.0	39.0	41.0	36.0	41.0
32	39.25275	41.0	39.0	41.0	36.0	41.0
33	38.56575	40.0	38.0	41.0	34.0	41.0
34	38.4965	40.0	38.0	41.0	34.0	41.0
35	38.08775	40.0	38.0	41.0	34.0	41.0
36	33.0425	37.0	30.0	40.0	18.0	41.0
37	35.5335	38.0	33.0	40.0	30.0	41.0
38	37.1155	39.0	37.0	40.0	32.0	41.0
39	37.1525	39.0	37.0	40.0	31.0	41.0
40	37.359	40.0	37.0	41.0	32.0	41.0
41	37.43225	40.0	37.0	41.0	33.0	41.0
42	37.73425	40.0	38.0	41.0	33.0	41.0
43	37.22475	40.0	37.0	41.0	32.0	41.0
44	37.4055	40.0	37.0	41.0	33.0	41.0
45	37.06375	40.0	37.0	41.0	31.0	41.0
46	35.18525	38.0	34.0	40.0	26.0	41.0
47	35.942	39.0	35.0	40.0	30.0	41.0
48	36.37025	39.0	37.0	41.0	31.0	41.0
49	35.1305	39.0	35.0	40.0	27.0	41.0
50	28.98775	33.0	24.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	1.0
17	1.0
18	1.0
19	1.0
20	2.0
21	7.0
22	6.0
23	6.0
24	13.0
25	16.0
26	14.0
27	20.0
28	32.0
29	33.0
30	39.0
31	60.0
32	69.0
33	99.0
34	155.0
35	209.0
36	315.0
37	496.0
38	1024.0
39	1379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.684948660155271	15.602304032056097	65.26421237165039	13.448534936138243
2	17.599999999999998	23.175	22.8	36.425000000000004
3	18.375	15.575	16.675	49.375
4	22.650000000000002	32.4	10.549999999999999	34.4
5	17.45	41.75	28.449999999999996	12.35
6	47.25	13.325000000000001	29.099999999999998	10.325
7	10.725	27.775	30.375000000000004	31.125000000000004
8	28.849999999999998	39.0	10.375	21.775
9	20.75	44.35	24.375	10.525
10	15.675	19.475	30.675	34.175
11	23.150000000000002	12.725	10.85	53.27499999999999
12	11.675	15.1	21.55	51.675000000000004
13	20.65	23.674999999999997	35.5	20.175
14	37.0	23.400000000000002	28.1	11.5
15	37.25	22.025	30.375000000000004	10.35
16	9.5	39.35	14.875	36.275
17	16.225	27.025	42.8	13.950000000000001
18	19.25	20.525	30.45	29.775000000000002
19	40.175	11.1	24.525	24.2
20	47.349999999999994	13.15	28.775000000000002	10.725
21	25.85	18.4	22.575	33.175
22	26.724999999999998	12.25	55.325	5.7
23	25.224999999999998	12.15	53.574999999999996	9.049999999999999
24	59.550000000000004	12.875	21.95	5.625
25	58.825	22.425	12.85	5.8999999999999995
26	22.7	57.574999999999996	11.25	8.475000000000001
27	11.525	58.199999999999996	22.900000000000002	7.375
28	7.425	22.5	58.375	11.700000000000001
29	6.4	10.549999999999999	65.525	17.525
30	5.825	5.475	35.15	53.55
31	12.15	3.6999999999999997	54.25	29.9
32	25.025	4.95	19.225	50.8
33	64.97500000000001	5.050000000000001	10.85	19.125
34	72.6	4.175	12.7	10.525
35	70.825	5.075	16.400000000000002	7.7
36	41.449999999999996	5.8500000000000005	41.9	10.8
37	52.6	6.575	18.675	22.15
38	19.3	12.075	10.274999999999999	58.35
39	11.899999999999999	23.025000000000002	6.075	59.0
40	12.4	58.775	6.525	22.3
41	22.35	60.175	5.949999999999999	11.525
42	59.85	28.15	6.2	5.800000000000001
43	59.824999999999996	28.7	4.925	6.550000000000001
44	25.3	59.175	4.9750000000000005	10.549999999999999
45	12.925	59.475	10.075000000000001	17.525
46	6.75	24.4	17.349999999999998	51.5
47	5.375	12.65	46.45	35.525
48	7.199999999999999	10.775	18.125	63.9
49	11.3	17.974999999999998	10.299999999999999	60.425
50	18.224999999999998	44.800000000000004	10.674999999999999	26.3
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	1.0
30	1.0
31	11.5
32	22.0
33	20.0
34	18.0
35	19.5
36	21.0
37	28.0
38	35.0
39	34.5
40	34.0
41	36.0
42	38.0
43	60.5
44	83.0
45	146.5
46	210.0
47	396.5
48	583.0
49	585.0
50	587.0
51	809.5
52	1032.0
53	725.5
54	419.0
55	409.0
56	399.0
57	360.0
58	321.0
59	193.0
60	65.0
61	47.5
62	30.0
63	47.0
64	64.0
65	44.0
66	24.0
67	14.0
68	4.0
69	3.0
70	2.0
71	1.5
72	1.0
73	2.0
74	3.0
75	1.5
76	0.0
77	0.5
78	1.0
79	1.5
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52554744525547	25.525
2	9.927007299270073	6.800000000000001
3	3.9416058394160585	4.05
4	2.7007299270072993	3.6999999999999997
5	1.897810218978102	3.25
6	0.948905109489051	1.95
7	0.7299270072992701	1.7500000000000002
8	0.36496350364963503	1.0
9	0.291970802919708	0.8999999999999999
>10	4.233576642335766	27.925
>50	0.291970802919708	6.875000000000001
>100	0.145985401459854	16.275000000000002
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	342	8.55	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	309	7.725	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	79	1.975	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	51	1.275	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	49	1.225	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	46	1.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	31	0.775	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	20	0.5	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC	16	0.4	RNA PCR Primer, Index 48 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACAGAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	9	0.22499999999999998	No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	8	0.2	RNA PCR Primer, Index 1 (96% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	7	0.17500000000000002	No Hit
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	7	0.17500000000000002	No Hit
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	6	0.15	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	6	0.15	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	5	0.125	RNA PCR Primer, Index 1 (96% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	5	0.125	No Hit
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TGGCTCAGTTCAGCAGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.5	0.0	0.0	0.0
2	0.0	0.525	0.0	0.0	0.0
3	0.0	0.575	0.0	0.0	0.0
4	0.0	0.6	0.0	0.0	0.0
5	0.0	0.625	0.0	0.0	0.0
6	0.0	0.625	0.0	0.0	0.0
7	0.0	0.75	0.0	0.0	0.0
8	0.0	0.85	0.0	0.0	0.0
9	0.0	1.05	0.0	0.0	0.0
10	0.0	1.225	0.0	0.0	0.0
11	0.0	1.3	0.0	0.0	0.0
12	0.0	1.55	0.0	0.0	0.0
13	0.0	1.75	0.0	0.0	0.0
14	0.0	2.0	0.0	0.0	0.0
15	0.0	2.225	0.0	0.0	0.0
16	0.0	2.525	0.0	0.0	0.0
17	0.0	3.0	0.0	0.0	0.0
18	0.0	3.475	0.0	0.0	0.0
19	0.0	4.05	0.0	0.0	0.0
20	0.0	5.325	0.0	0.0	0.0
21	0.0	11.075	0.0	0.0	0.0
22	0.0	23.35	0.0	0.0	0.0
23	0.0	64.625	0.0	0.0	0.0
24	0.0	77.675	0.0	0.0	0.0
25	0.0	83.0	0.0	0.0	0.0
26	0.0	83.975	0.0	0.0	0.0
27	0.0	84.225	0.0	0.0	0.0
28	0.0	84.575	0.0	0.0	0.0
29	0.0	84.875	0.0	0.0	0.0
30	0.0	85.225	0.0	0.0	0.0
31	0.0	85.725	0.0	0.0	0.0
32	0.0	86.65	0.0	0.0	0.0
33	0.0	87.325	0.0	0.0	0.0
34	0.0	88.8	0.0	0.0	0.0
35	0.0	89.1	0.0	0.0	0.0
36	0.0	89.55	0.0	0.0	0.0
37	0.0	89.925	0.0	0.0	0.0
38	0.0	90.025	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
AAGTAAT	45	2.728484E-10	44.000004	4
TCAAGTA	45	2.728484E-10	44.000004	2
AACCGGT	20	6.952051E-4	44.000004	9
AATCCAG	45	2.728484E-10	44.000004	8
ATCCAGG	45	2.728484E-10	44.000004	9
CTTTAAC	80	0.0	44.000004	12
TTCAAGT	45	2.728484E-10	44.000004	1
AGGATAG	45	2.728484E-10	44.000004	13
GGTCTCT	20	6.952051E-4	44.000004	13
ATAGGCT	40	5.315087E-9	44.000004	16
GAGCTTG	20	6.952051E-4	44.000004	13
GGATCCG	20	6.952051E-4	44.000004	3
CCGTCTG	20	6.952051E-4	44.000004	7
TCGGATC	20	6.952051E-4	44.000004	1
CGGATCC	20	6.952051E-4	44.000004	2
TCCAGGA	45	2.728484E-10	44.000004	10
GAGTTGG	20	6.952051E-4	44.000004	20
GGATAGG	45	2.728484E-10	44.000004	14
>>END_MODULE
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86584 spots for SRR5030903.sra
Written 86584 spots for SRR5030903.sra
Read 86603 spots for SRR5030903.sra
Written 86603 spots for SRR5030903.sra
SRR ids: ['SRR5030903.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_2na4eas1
SRR5030903.sra spots: 2770707
blocks: [[1, 86584], [86585, 173168], [173169, 259752], [259753, 346336], [346337, 432920], [432921, 519504], [519505, 606088], [606089, 692672], [692673, 779256], [779257, 865840], [865841, 952424], [952425, 1039008], [1039009, 1125592], [1125593, 1212176], [1212177, 1298760], [1298761, 1385344], [1385345, 1471928], [1471929, 1558512], [1558513, 1645096], [1645097, 1731680], [1731681, 1818264], [1818265, 1904848], [1904849, 1991432], [1991433, 2078016], [2078017, 2164600], [2164601, 2251184], [2251185, 2337768], [2337769, 2424352], [2424353, 2510936], [2510937, 2597520], [2597521, 2684104], [2684105, 2770707]]
SRR5030903 file size 479144
SRR5030903 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 32 -o SRR5030903 SRR5030903_1.fastq
Input file:	SRR5030903_1.fastq
trimmed:	SRR5030903-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Sun Jan 12 06:08:45 2020 >> started

Sun Jan 12 06:08:47 2020 >> done (2.470s)
2770707 reads processed; of these:
     94 ( 0.00%) short reads filtered out after trimming by size control
      7 ( 0.00%) empty reads filtered out after trimming by size control
2770606 (100.00%) reads available; of these:
 162410 ( 5.86%) trimmed reads available after processing
2608196 (94.14%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     35	  0.00%
 19	     41	  0.00%
 20	     83	  0.00%
 21	    107	  0.00%
 22	    221	  0.01%
 23	    272	  0.01%
 24	    227	  0.01%
 25	    305	  0.01%
 26	    255	  0.01%
 27	    328	  0.01%
 28	    509	  0.02%
 29	    837	  0.03%
 30	    556	  0.02%
 31	    658	  0.02%
 32	    669	  0.02%
 33	    942	  0.03%
 34	   2886	  0.10%
 35	   2407	  0.09%
 36	   1592	  0.06%
 37	   1664	  0.06%
 38	   1805	  0.07%
 39	   2039	  0.07%
 40	   2385	  0.09%
 41	   2384	  0.09%
 42	   3982	  0.14%
 43	   4220	  0.15%
 44	   9382	  0.34%
 45	  11351	  0.41%
 46	   9640	  0.35%
 47	  14337	  0.52%
 48	  35702	  1.29%
 49	  50589	  1.83%
 50	2608196	 94.14%
2770606 reads passed initial QC


criterion=sequence-density
sequence-density=89.61
sequence-density-rank=1
fanout-score=15.84
fanout-score-rank=2
prefix-density=90.83
prefix-fanout=15.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC


criterion=fanout-score
sequence-density=1.49
sequence-density-rank=12
fanout-score=173.25
fanout-score-rank=1
prefix-density=90.48
prefix-fanout=2.9
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 32 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC -o SRR5030903 -
Input file:	STDIN
trimmed:	SRR5030903-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Sun Jan 12 06:09:02 2020 >> started

Sun Jan 12 06:09:06 2020 >> done (3.688s)
2709037 reads processed; of these:
  78760 ( 2.91%) short reads filtered out after trimming by size control
  12115 ( 0.45%) empty reads filtered out after trimming by size control
2618162 (96.65%) reads available; of these:
2477492 (94.63%) trimmed reads available after processing
 140670 ( 5.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15651	  0.60%
 19	  31124	  1.19%
 20	 152696	  5.83%
 21	 349980	 13.37%
 22	1100953	 42.05%
 23	 387519	 14.80%
 24	 145340	  5.55%
 25	  26847	  1.03%
 26	  10995	  0.42%
 27	   9102	  0.35%
 28	   9915	  0.38%
 29	   6881	  0.26%
 30	  12963	  0.50%
 31	  19476	  0.74%
 32	  16297	  0.62%
 33	  40442	  1.54%
 34	  15093	  0.58%
 35	  11414	  0.44%
 36	  10696	  0.41%
 37	   6903	  0.26%
 38	   8401	  0.32%
 39	   8730	  0.33%
 40	  11659	  0.45%
 41	  15313	  0.58%
 42	  14910	  0.57%
 43	  11026	  0.42%
 44	   9852	  0.38%
 45	  11338	  0.43%
 46	   9432	  0.36%
 47	   6230	  0.24%
 48	   1678	  0.06%
 49	   7041	  0.27%
 50	 122265	  4.67%


criterion=sequence-density
sequence-density=5.11
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTT


criterion=fanout-score
sequence-density=0.53
sequence-density-rank=25
fanout-score=15.58
fanout-score-rank=1
prefix-density=8.19
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Jan 12 06:09:33
                             Started mapping on |	Jan 12 06:09:33
                                    Finished on |	Jan 12 06:10:01
       Mapping speed, Million of reads per hour |	344.54

                          Number of input reads |	2679731
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1533677
                        Uniquely mapped reads % |	57.23%
                          Average mapped length |	24.68
                       Number of splices: Total |	819
            Number of splices: Annotated (sjdb) |	99
                       Number of splices: GT/AG |	744
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	59
                      Mismatch rate per base, % |	0.13%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.53
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	967894
             % of reads mapped to multiple loci |	36.12%
        Number of reads mapped to too many loci |	32784
             % of reads mapped to too many loci |	1.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.12%
                     % of reads unmapped: other |	0.31%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	178160	178160	178160
N_multimapping	967894	967894	967894
N_noFeature	43982	53145	1174784
N_ambiguous	924554	625762	6099
UnstrandedReadsAssigned:565141 PositiveStrandReadsAssigned:854770 NegativeStrandReadsAssigned:352794
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030903 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030903-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,679,731 reads, 721,987 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,105 rounds

 109283 SRR5030903.ke.tsv
  52636 SRR5030903.se.tsv
 161919 total
==> SRR5030903.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030903.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030903 completed mapping pipeline successfully
