Starting /dee2/code/volunteer_pipeline.sh SRR5030847
    current disk space = 160678363136
    free memory = 56720396 
SRR5030847 SRAfilesize
198b4bf317d047e29708c8b4c70eba4c  SRR5030847.sra
SRR5030847.sra file validated
SRR5030847 is single end
SRR5030847 is conventional basespace
SRR5030847 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.492	34.0	31.0	34.0	31.0	34.0
2	32.78125	34.0	31.0	34.0	31.0	34.0
3	32.79575	34.0	31.0	34.0	31.0	34.0
4	36.287	37.0	37.0	37.0	35.0	37.0
5	36.35925	37.0	37.0	37.0	35.0	37.0
6	36.12225	37.0	37.0	37.0	35.0	37.0
7	36.06525	37.0	35.0	37.0	35.0	37.0
8	36.17725	37.0	36.0	37.0	35.0	37.0
9	37.9855	39.0	38.0	39.0	35.0	39.0
10	37.957	39.0	38.0	39.0	35.0	39.0
11	37.90375	39.0	38.0	39.0	35.0	39.0
12	37.82525	39.0	38.0	39.0	35.0	39.0
13	37.87675	39.0	38.0	39.0	35.0	39.0
14	39.18425	41.0	39.0	41.0	36.0	41.0
15	39.20975	41.0	39.0	41.0	36.0	41.0
16	39.2015	40.0	39.0	41.0	36.0	41.0
17	39.27975	41.0	39.0	41.0	36.0	41.0
18	39.463	41.0	39.0	41.0	36.0	41.0
19	39.445	41.0	39.0	41.0	36.0	41.0
20	39.22325	41.0	39.0	41.0	36.0	41.0
21	39.22775	41.0	39.0	41.0	36.0	41.0
22	39.373	41.0	39.0	41.0	36.0	41.0
23	39.32825	41.0	39.0	41.0	36.0	41.0
24	38.4835	40.0	38.0	41.0	34.0	41.0
25	38.83525	41.0	39.0	41.0	34.0	41.0
26	38.972	41.0	39.0	41.0	35.0	41.0
27	39.07025	41.0	39.0	41.0	35.0	41.0
28	38.7525	40.0	38.0	41.0	34.0	41.0
29	38.208	40.0	38.0	41.0	33.0	41.0
30	38.3545	40.0	38.0	41.0	33.0	41.0
31	38.824	40.0	38.0	41.0	35.0	41.0
32	38.8445	40.0	38.0	41.0	35.0	41.0
33	37.78825	40.0	38.0	41.0	33.0	41.0
34	37.79175	40.0	38.0	41.0	32.0	41.0
35	37.7165	40.0	37.0	41.0	33.0	41.0
36	33.58225	38.0	30.0	40.0	18.0	41.0
37	35.85575	38.0	34.0	40.0	30.0	41.0
38	37.1325	39.0	37.0	40.0	32.0	41.0
39	37.22425	39.0	37.0	40.0	32.0	41.0
40	37.2195	39.0	37.0	41.0	32.0	41.0
41	37.0365	39.0	36.0	40.0	31.0	41.0
42	36.913	39.0	36.0	40.0	31.0	41.0
43	37.04125	39.0	36.0	41.0	32.0	41.0
44	37.265	40.0	37.0	41.0	32.0	41.0
45	36.82225	39.0	36.0	41.0	31.0	41.0
46	34.5085	38.0	33.0	40.0	24.0	41.0
47	35.388	38.0	34.0	40.0	28.0	41.0
48	35.53	38.0	35.0	40.0	29.0	41.0
49	34.7885	38.0	34.0	40.0	27.0	41.0
50	30.85025	34.0	29.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	3.0
17	0.0
18	1.0
19	1.0
20	1.0
21	9.0
22	4.0
23	9.0
24	11.0
25	22.0
26	15.0
27	31.0
28	32.0
29	47.0
30	56.0
31	52.0
32	75.0
33	102.0
34	181.0
35	188.0
36	300.0
37	540.0
38	1037.0
39	1281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.693713999499122	26.471324818432258	47.3328324567994	15.502128725269221
2	20.825	27.35	21.2	30.625000000000004
3	23.95	21.175	23.400000000000002	31.474999999999998
4	30.5	28.075	14.75	26.674999999999997
5	21.3	41.4	24.425	12.875
6	33.85	16.725	33.525	15.9
7	17.875	20.474999999999998	25.924999999999997	35.725
8	27.025	30.15	14.374999999999998	28.449999999999996
9	19.775000000000002	33.975	30.2	16.05
10	25.025	20.474999999999998	28.199999999999996	26.3
11	31.2	13.275	17.549999999999997	37.974999999999994
12	16.325	18.425	30.349999999999998	34.9
13	31.0	19.675	29.7	19.625
14	30.95	30.775000000000002	20.974999999999998	17.299999999999997
15	31.2	27.05	27.900000000000002	13.850000000000001
16	17.599999999999998	23.825	22.95	35.625
17	23.525	22.125	38.0	16.35
18	24.275	19.55	29.725	26.450000000000003
19	35.0	14.75	30.55	19.7
20	37.574999999999996	18.725	28.175	15.525
21	24.025	20.724999999999998	26.474999999999998	28.775000000000002
22	27.950000000000003	15.55	43.625	12.875
23	26.625	15.275	39.225	18.875
24	47.699999999999996	16.375	24.85	11.075
25	47.3	23.3	16.400000000000002	13.0
26	28.7	42.525	16.05	12.725
27	17.599999999999998	45.2	22.75	14.45
28	13.850000000000001	25.5	44.65	16.0
29	11.700000000000001	15.2	53.725	19.375
30	11.725	9.35	34.2	44.725
31	17.125	7.225	43.925	31.724999999999998
32	27.200000000000003	8.674999999999999	22.3	41.825
33	49.025	10.775	15.6	24.6
34	55.00000000000001	9.375	18.7	16.925
35	59.35	8.799999999999999	18.9	12.950000000000001
36	43.425000000000004	9.275	32.025	15.275
37	42.35	12.225	20.8	24.625
38	24.725	16.775000000000002	14.399999999999999	44.1
39	18.575	23.275000000000002	11.55	46.6
40	17.549999999999997	43.725	15.4	23.325000000000003
41	25.575	45.324999999999996	11.700000000000001	17.4
42	46.225	28.675	13.850000000000001	11.25
43	47.15	29.299999999999997	12.15	11.4
44	30.975	45.074999999999996	10.0	13.950000000000001
45	19.825	47.075	15.174999999999999	17.925
46	15.174999999999999	27.950000000000003	19.575	37.3
47	11.675	17.625	37.25	33.45
48	14.099999999999998	14.099999999999998	21.625	50.175000000000004
49	15.45	18.95	17.525	48.075
50	19.979994998749685	33.9584896224056	16.204051012753187	29.857464366091524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	1.5
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	1.5
20	2.0
21	1.5
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	2.0
28	4.0
29	5.0
30	6.0
31	22.0
32	38.0
33	30.0
34	22.0
35	33.0
36	44.0
37	58.5
38	73.0
39	68.5
40	64.0
41	72.0
42	80.0
43	97.0
44	114.0
45	161.0
46	208.0
47	323.0
48	438.0
49	535.5
50	633.0
51	739.0
52	845.0
53	636.0
54	427.0
55	415.0
56	403.0
57	304.5
58	206.0
59	139.5
60	73.0
61	53.0
62	33.0
63	63.0
64	93.0
65	62.0
66	31.0
67	22.5
68	14.0
69	23.5
70	33.0
71	59.5
72	86.0
73	49.0
74	12.0
75	6.5
76	1.0
77	3.0
78	5.0
79	2.5
80	0.0
81	2.5
82	5.0
83	2.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.675000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56508698260348	31.075000000000003
2	10.617876424715057	8.85
3	4.139172165566887	5.175
4	2.2195560887822436	3.6999999999999997
5	1.6196760647870425	3.375
6	1.3797240551889622	3.45
7	0.6598680263947211	1.925
8	0.5398920215956808	1.7999999999999998
9	0.6598680263947211	2.475
>10	3.119376124775045	22.775000000000002
>50	0.4199160167966407	12.475
>100	0.05998800239952009	2.9250000000000003
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	117	2.9250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	77	1.925	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	76	1.9	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	69	1.725	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	46	1.15	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	36	0.8999999999999999	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	25	0.625	RNA PCR Primer, Index 7 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	24	0.6	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC	22	0.5499999999999999	RNA PCR Primer, Index 10 (100% over 44bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	20	0.5	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	18	0.44999999999999996	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	17	0.42500000000000004	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	16	0.4	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	15	0.375	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 10 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	13	0.325	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	12	0.3	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	12	0.3	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	12	0.3	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 25bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	11	0.27499999999999997	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	10	0.25	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	9	0.22499999999999998	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	9	0.22499999999999998	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	9	0.22499999999999998	No Hit
TAATACTGTCTGGTAATGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	9	0.22499999999999998	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	8	0.2	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	8	0.2	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	8	0.2	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	7	0.17500000000000002	RNA PCR Primer, Index 3 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	6	0.15	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATGGAAT	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACACT	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGCC	5	0.125	No Hit
GGTCCATGATGAAGCCCATTATCAGTGGACATCTATGGATGAGAAATGCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.575	0.0	0.0	0.0
5	0.0	0.65	0.0	0.0	0.0
6	0.0	0.725	0.0	0.0	0.0
7	0.0	1.425	0.0	0.0	0.0
8	0.0	1.55	0.0	0.0	0.0
9	0.0	1.625	0.0	0.0	0.0
10	0.0	1.725	0.0	0.0	0.0
11	0.0	1.85	0.0	0.0	0.0
12	0.0	2.0	0.0	0.0	0.0
13	0.0	2.225	0.0	0.0	0.0
14	0.0	2.45	0.0	0.0	0.0
15	0.0	2.825	0.0	0.0	0.0
16	0.0	3.8	0.0	0.0	0.0
17	0.0	4.175	0.0	0.0	0.0
18	0.0	5.0	0.0	0.0	0.0
19	0.0	5.525	0.0	0.0	0.0
20	0.0	6.375	0.0	0.0	0.0
21	0.0	11.3	0.0	0.0	0.0
22	0.0	20.925	0.0	0.0	0.0
23	0.0	46.525	0.0	0.0	0.0
24	0.0	57.9	0.0	0.0	0.0
25	0.0	63.5	0.0	0.0	0.0
26	0.0	65.3	0.0	0.0	0.0
27	0.0	65.95	0.0	0.0	0.0
28	0.0	66.525	0.0	0.0	0.0
29	0.0	67.075	0.0	0.0	0.0
30	0.0	67.6	0.0	0.0	0.0
31	0.0	68.15	0.0	0.0	0.0
32	0.0	68.975	0.0	0.0	0.0
33	0.0	69.7	0.0	0.0	0.0
34	0.0	72.475	0.0	0.0	0.0
35	0.0	73.625	0.0	0.0	0.0
36	0.0	74.525	0.0	0.0	0.0
37	0.0	75.65	0.0	0.0	0.0
38	0.0	76.225	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AACCGGT	20	6.952051E-4	44.000004	9
ATCCAGG	20	6.952051E-4	44.000004	9
AGTAGGT	20	6.952051E-4	44.000004	7
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
GGTCTCT	20	6.952051E-4	44.000004	13
TCCAGGA	20	6.952051E-4	44.000004	10
GGATAGG	20	6.952051E-4	44.000004	14
TAGTAGG	20	6.952051E-4	44.000004	6
AGTAATC	20	6.952051E-4	44.000004	5
GAACCGG	20	6.952051E-4	44.000004	8
GTCTCTT	20	6.952051E-4	44.000004	14
GGCTTGG	20	6.952051E-4	44.000004	19
TAATCCA	20	6.952051E-4	44.000004	7
AGTGAAC	20	6.952051E-4	44.000004	5
GTAGGTT	20	6.952051E-4	44.000004	8
>>END_MODULE
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
Read 173261 spots for SRR5030847.sra
Written 173261 spots for SRR5030847.sra
Read 173260 spots for SRR5030847.sra
Written 173260 spots for SRR5030847.sra
SRR ids: ['SRR5030847.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_56lr6p7m
SRR5030847.sra spots: 2079121
blocks: [[1, 173260], [173261, 346520], [346521, 519780], [519781, 693040], [693041, 866300], [866301, 1039560], [1039561, 1212820], [1212821, 1386080], [1386081, 1559340], [1559341, 1732600], [1732601, 1905860], [1905861, 2079121]]
SRR5030847 file size 359277
SRR5030847 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030847 SRR5030847_1.fastq
Input file:	SRR5030847_1.fastq
trimmed:	SRR5030847-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:50 2020 >> started

Wed Mar  4 06:26:52 2020 >> done (1.730s)
2079121 reads processed; of these:
    112 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2079003 (99.99%) reads available; of these:
 151272 ( 7.28%) trimmed reads available after processing
1927731 (92.72%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     28	  0.00%
 19	     24	  0.00%
 20	    109	  0.01%
 21	    123	  0.01%
 22	    220	  0.01%
 23	    258	  0.01%
 24	    242	  0.01%
 25	    346	  0.02%
 26	    322	  0.02%
 27	    396	  0.02%
 28	    497	  0.02%
 29	    734	  0.04%
 30	    548	  0.03%
 31	    699	  0.03%
 32	   1015	  0.05%
 33	   1637	  0.08%
 34	   5074	  0.24%
 35	   2358	  0.11%
 36	   1828	  0.09%
 37	   1947	  0.09%
 38	   2812	  0.14%
 39	   2596	  0.12%
 40	   4043	  0.19%
 41	   3026	  0.15%
 42	   5070	  0.24%
 43	   4122	  0.20%
 44	  10600	  0.51%
 45	  10338	  0.50%
 46	  10069	  0.48%
 47	  12306	  0.59%
 48	  28629	  1.38%
 49	  39256	  1.89%
 50	1927731	 92.72%
2079003 reads passed initial QC


criterion=sequence-density
sequence-density=75.73
sequence-density-rank=1
fanout-score=21.33
fanout-score-rank=2
prefix-density=78.13
prefix-fanout=20.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC


criterion=fanout-score
sequence-density=1.90
sequence-density-rank=7
fanout-score=124.69
fanout-score-rank=1
prefix-density=77.70
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC -o SRR5030847 -
Input file:	STDIN
trimmed:	SRR5030847-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:27:04 2020 >> started

Wed Mar  4 06:27:08 2020 >> done (3.229s)
2024293 reads processed; of these:
 114900 ( 5.68%) short reads filtered out after trimming by size control
   8758 ( 0.43%) empty reads filtered out after trimming by size control
1900635 (93.89%) reads available; of these:
1637375 (86.15%) trimmed reads available after processing
 263260 (13.85%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  10164	  0.53%
 19	  18111	  0.95%
 20	  95583	  5.03%
 21	 216590	 11.40%
 22	 512858	 26.98%
 23	 219778	 11.56%
 24	 113472	  5.97%
 25	  32997	  1.74%
 26	  15750	  0.83%
 27	  11202	  0.59%
 28	  11760	  0.62%
 29	   9972	  0.52%
 30	  14308	  0.75%
 31	  18892	  0.99%
 32	  21041	  1.11%
 33	  52663	  2.77%
 34	  22077	  1.16%
 35	  18524	  0.97%
 36	  22681	  1.19%
 37	  11584	  0.61%
 38	  12240	  0.64%
 39	  12985	  0.68%
 40	  19646	  1.03%
 41	  26464	  1.39%
 42	  32808	  1.73%
 43	  22882	  1.20%
 44	  18572	  0.98%
 45	  25694	  1.35%
 46	  20936	  1.10%
 47	  16281	  0.86%
 48	   3034	  0.16%
 49	   8427	  0.44%
 50	 230659	 12.14%


criterion=sequence-density
sequence-density=4.86
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=184.71
fanout-score-rank=1
prefix-density=1.38
prefix-fanout=1.9
sequence=TTGTATAGTTTAACAGTCTACAGCCATGGTCGTGGAATTCT
                                 Started job on |	Mar 04 06:27:30
                             Started mapping on |	Mar 04 06:27:30
                                    Finished on |	Mar 04 06:27:54
       Mapping speed, Million of reads per hour |	293.30

                          Number of input reads |	1955345
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1111234
                        Uniquely mapped reads % |	56.83%
                          Average mapped length |	28.24
                       Number of splices: Total |	916
            Number of splices: Annotated (sjdb) |	178
                       Number of splices: GT/AG |	837
                       Number of splices: GC/AG |	14
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	65
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	570572
             % of reads mapped to multiple loci |	29.18%
        Number of reads mapped to too many loci |	48761
             % of reads mapped to too many loci |	2.49%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.88%
                     % of reads unmapped: other |	0.62%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	273539	273539	273539
N_multimapping	570572	570572	570572
N_noFeature	53912	69337	827110
N_ambiguous	574053	333820	14929
UnstrandedReadsAssigned:483269 PositiveStrandReadsAssigned:708077 NegativeStrandReadsAssigned:269195
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030847 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030847-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,955,345 reads, 473,503 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,120 rounds

 109283 SRR5030847.ke.tsv
  52636 SRR5030847.se.tsv
 161919 total
==> SRR5030847.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030847.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030847 completed mapping pipeline successfully
