Starting /dee2/code/volunteer_pipeline.sh SRR5031014
    current disk space = 160552878080
    free memory = 73946028 
Starting /dee2/code/volunteer_pipeline.sh SRR5031014
    current disk space = 160552878080
    free memory = 73936368 
SRR5031014 failed download with prefetch
SRR5031014 SRAfilesize
SRR5031014.sra md5sums do not match. Deleting and exiting
SRR5031014 SRAfilesize
57d5567af103128333e922e6e5a40367  SRR5031014.sra
SRR5031014.sra file validated
SRR5031014 is single end
SRR5031014 is conventional basespace
SRR5031014 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2185	33.0	31.0	34.0	30.0	34.0
2	32.47	34.0	31.0	34.0	31.0	34.0
3	32.49775	34.0	31.0	34.0	31.0	34.0
4	36.07675	37.0	35.0	37.0	35.0	37.0
5	36.05075	37.0	35.0	37.0	35.0	37.0
6	35.8245	37.0	35.0	37.0	35.0	37.0
7	35.79275	37.0	35.0	37.0	35.0	37.0
8	35.90075	37.0	35.0	37.0	35.0	37.0
9	37.51325	39.0	37.0	39.0	35.0	39.0
10	37.4025	39.0	37.0	39.0	34.0	39.0
11	37.06125	39.0	37.0	39.0	33.0	39.0
12	37.4535	39.0	37.0	39.0	34.0	39.0
13	37.6095	39.0	37.0	39.0	35.0	39.0
14	38.8655	40.0	38.0	41.0	35.0	41.0
15	38.74275	40.0	38.0	41.0	34.0	41.0
16	38.85675	40.0	38.0	41.0	35.0	41.0
17	39.019	40.0	38.0	41.0	36.0	41.0
18	38.8475	40.0	38.0	41.0	35.0	41.0
19	38.764	40.0	38.0	41.0	34.0	41.0
20	38.633	40.0	38.0	41.0	34.0	41.0
21	38.777	40.0	38.0	41.0	34.0	41.0
22	38.654	40.0	38.0	41.0	34.0	41.0
23	38.342	40.0	38.0	41.0	34.0	41.0
24	38.65	40.0	38.0	41.0	34.0	41.0
25	38.8755	40.0	38.0	41.0	35.0	41.0
26	38.672	40.0	38.0	41.0	34.0	41.0
27	38.763	40.0	38.0	41.0	34.0	41.0
28	38.451	40.0	38.0	41.0	34.0	41.0
29	38.25475	40.0	38.0	41.0	33.0	41.0
30	38.1265	40.0	38.0	41.0	33.0	41.0
31	38.378	40.0	38.0	41.0	34.0	41.0
32	38.315	40.0	38.0	41.0	34.0	41.0
33	37.95075	40.0	38.0	41.0	33.0	41.0
34	37.87525	40.0	37.0	41.0	33.0	41.0
35	37.622	40.0	37.0	41.0	32.0	41.0
36	37.49	39.0	37.0	41.0	32.0	41.0
37	37.03425	39.0	36.0	41.0	31.0	41.0
38	37.153	39.0	36.0	40.0	31.0	41.0
39	37.4595	39.0	37.0	41.0	32.0	41.0
40	37.59325	40.0	37.0	41.0	33.0	41.0
41	37.71975	40.0	37.0	41.0	33.0	41.0
42	37.711	40.0	37.0	41.0	32.0	41.0
43	37.6155	40.0	37.0	41.0	33.0	41.0
44	37.7625	40.0	37.0	41.0	33.0	41.0
45	37.56325	40.0	37.0	41.0	33.0	41.0
46	37.23975	40.0	37.0	41.0	32.0	41.0
47	36.607	40.0	35.0	41.0	30.0	41.0
48	36.3665	39.0	35.0	41.0	30.0	41.0
49	36.38575	39.0	35.0	41.0	30.0	41.0
50	35.78475	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	1.0
16	0.0
17	3.0
18	1.0
19	1.0
20	2.0
21	4.0
22	5.0
23	11.0
24	5.0
25	12.0
26	14.0
27	31.0
28	39.0
29	36.0
30	61.0
31	60.0
32	79.0
33	102.0
34	178.0
35	205.0
36	294.0
37	474.0
38	753.0
39	1627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.7	24.3	49.375	15.625
2	22.681704260651628	27.29323308270677	20.075187969924812	29.949874686716793
3	26.0	17.925	24.275	31.8
4	35.175	29.599999999999998	14.625	20.599999999999998
5	22.35	38.375	25.474999999999998	13.8
6	29.025000000000002	17.025000000000002	39.925	14.025000000000002
7	18.55	19.950000000000003	20.349999999999998	41.15
8	21.15	28.95	17.5	32.4
9	25.924999999999997	30.45	26.950000000000003	16.675
10	22.375	17.65	32.800000000000004	27.175
11	30.925000000000004	11.774999999999999	16.5	40.8
12	16.225	19.475	34.175	30.125
13	29.825000000000003	17.0	30.599999999999998	22.575
14	29.849999999999998	31.5	19.975	18.675
15	37.7	22.625	24.8	14.875
16	17.599999999999998	19.6	24.675	38.125
17	26.650000000000002	21.375	36.975	15.0
18	26.224999999999998	17.125	33.025	23.625
19	37.525	16.0	30.125	16.35
20	44.800000000000004	16.525000000000002	22.225	16.45
21	22.725	22.475	24.474999999999998	30.325000000000003
22	29.5	14.975	44.25	11.275
23	26.025	14.499999999999998	42.175000000000004	17.299999999999997
24	49.15	14.75	27.075	9.025
25	48.699999999999996	23.599999999999998	17.025000000000002	10.674999999999999
26	28.325	44.6	14.85	12.225
27	15.9	44.3	24.325	15.475
28	12.325	23.3	47.475	16.900000000000002
29	12.775	13.3	54.225	19.7
30	11.675	7.3999999999999995	35.449999999999996	45.475
31	16.425	5.5	44.1	33.975
32	27.775	7.775	21.3	43.15
33	52.37500000000001	9.125	14.35	24.15
34	57.95	7.925	16.825000000000003	17.299999999999997
35	62.150000000000006	8.175	18.975	10.7
36	40.025	8.7	36.225	15.049999999999999
37	44.625	11.55	20.0	23.825
38	24.825	16.775000000000002	12.675	45.725
39	17.075000000000003	24.15	10.575	48.199999999999996
40	16.925	47.05	12.15	23.875
41	25.374999999999996	45.85	11.125	17.65
42	45.85	28.799999999999997	13.575000000000001	11.774999999999999
43	50.64999999999999	28.725	9.2	11.425
44	30.625000000000004	44.75	9.325	15.299999999999999
45	19.15	46.675	14.025000000000002	20.150000000000002
46	15.0	25.95	17.65	41.4
47	11.0	15.8	37.75	35.449999999999996
48	12.65	12.3	20.875	54.175
49	15.225	18.6	14.85	51.324999999999996
50	19.175	36.625	13.325000000000001	30.875000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	1.5
28	3.0
29	1.5
30	0.0
31	6.0
32	12.0
33	18.5
34	25.0
35	25.0
36	25.0
37	30.0
38	35.0
39	37.0
40	39.0
41	49.0
42	59.0
43	80.0
44	101.0
45	146.0
46	191.0
47	292.0
48	393.0
49	498.0
50	603.0
51	695.0
52	787.0
53	619.0
54	451.0
55	522.0
56	593.0
57	433.0
58	273.0
59	180.5
60	88.0
61	70.5
62	53.0
63	72.0
64	91.0
65	65.0
66	39.0
67	30.5
68	22.0
69	23.5
70	25.0
71	51.0
72	77.0
73	40.5
74	4.0
75	3.5
76	3.0
77	2.5
78	2.0
79	2.5
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.25
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52314521242866	29.775000000000002
2	9.765377298668358	7.7
3	4.311984781230184	5.1
4	1.9657577679137603	3.1
5	1.4584654407102093	2.875
6	1.5218769816106532	3.5999999999999996
7	0.8243500317057704	2.275
8	0.3804692454026633	1.2
9	0.3804692454026633	1.35
>10	3.3608116677235254	23.1
>50	0.31705770450221943	10.5
>100	0.19023462270133165	9.425
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	162	4.05	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	113	2.825	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	102	2.55	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	99	2.475	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	95	2.375	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	73	1.825	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	49	1.225	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	40	1.0	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	39	0.975	RNA PCR Primer, Index 7 (100% over 35bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	39	0.975	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGC	25	0.625	RNA PCR Primer, Index 34 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	17	0.42500000000000004	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	16	0.4	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	12	0.3	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTC	12	0.3	RNA PCR Primer, Index 34 (100% over 44bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	11	0.27499999999999997	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	9	0.22499999999999998	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	8	0.2	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGCC	8	0.2	No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	7	0.17500000000000002	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 24bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT	6	0.15	RNA PCR Primer, Index 9 (97% over 36bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	6	0.15	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGATCAGAAGGTGACTGTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	6	0.15	No Hit
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	6	0.15	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCTGGAATTCTCGGGTG	5	0.125	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CATGCTCAATAGGATTACGCTGAGGCCCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.725	0.0	0.0	0.0
2	0.0	0.725	0.0	0.0	0.0
3	0.0	0.825	0.0	0.0	0.0
4	0.0	0.925	0.0	0.0	0.0
5	0.0	1.0	0.0	0.0	0.0
6	0.0	1.1	0.0	0.0	0.0
7	0.0	1.45	0.0	0.0	0.0
8	0.0	1.575	0.0	0.0	0.0
9	0.0	1.675	0.0	0.0	0.0
10	0.0	1.75	0.0	0.0	0.0
11	0.0	1.925	0.0	0.0	0.0
12	0.0	2.3	0.0	0.0	0.0
13	0.0	2.575	0.0	0.0	0.0
14	0.0	2.75	0.0	0.0	0.0
15	0.0	3.375	0.0	0.0	0.0
16	0.0	4.925	0.0	0.0	0.0
17	0.0	5.425	0.0	0.0	0.0
18	0.0	6.7	0.0	0.0	0.0
19	0.0	7.35	0.0	0.0	0.0
20	0.0	8.45	0.0	0.0	0.0
21	0.0	13.725	0.0	0.0	0.0
22	0.0	24.4	0.0	0.0	0.0
23	0.0	52.025	0.0	0.0	0.0
24	0.0	64.1	0.0	0.0	0.0
25	0.0	70.0	0.0	0.0	0.0
26	0.0	71.275	0.0	0.0	0.0
27	0.0	72.15	0.0	0.0	0.0
28	0.0	72.65	0.0	0.0	0.0
29	0.0	73.775	0.0	0.0	0.0
30	0.0	74.275	0.0	0.0	0.0
31	0.0	75.2	0.0	0.0	0.0
32	0.0	76.25	0.0	0.0	0.0
33	0.0	77.35	0.0	0.0	0.0
34	0.0	80.325	0.0	0.0	0.0
35	0.0	81.825	0.0	0.0	0.0
36	0.0	83.175	0.0	0.0	0.0
37	0.0	84.325	0.0	0.0	0.0
38	0.0	84.975	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	40	5.315087E-9	44.000004	8
ACGCTGT	40	5.315087E-9	44.000004	9
GAGTGGA	20	6.952051E-4	44.000004	20
TTCAACG	40	5.315087E-9	44.000004	5
AGTGGAA	20	6.952051E-4	44.000004	21
CATTCAA	40	5.315087E-9	44.000004	3
TCAACGC	40	5.315087E-9	44.000004	6
GTGAGTG	20	6.952051E-4	44.000004	18
CTTGGTG	20	6.952051E-4	44.000004	16
ACATTCA	40	5.315087E-9	44.000004	2
CTTGGCT	40	5.315087E-9	44.000004	16
GCTGTCG	40	5.315087E-9	44.000004	11
GCTTGGT	20	6.952051E-4	44.000004	15
GCTTGGC	45	2.728484E-10	44.000004	15
TTGGCTT	20	6.952051E-4	44.000004	17
TTGGCTG	20	6.952051E-4	44.000004	17
GGTGGAA	20	6.952051E-4	44.000004	19
CTGTCGG	40	5.315087E-9	44.000004	12
TGGTGGA	20	6.952051E-4	44.000004	18
TGAGTGG	20	6.952051E-4	44.000004	19
>>END_MODULE
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275251 spots for SRR5031014.sra
Written 275251 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
Read 275250 spots for SRR5031014.sra
Written 275250 spots for SRR5031014.sra
SRR ids: ['SRR5031014.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_58gibww_
SRR5031014.sra spots: 3303001
blocks: [[1, 275250], [275251, 550500], [550501, 825750], [825751, 1101000], [1101001, 1376250], [1376251, 1651500], [1651501, 1926750], [1926751, 2202000], [2202001, 2477250], [2477251, 2752500], [2752501, 3027750], [3027751, 3303001]]
SRR5031014 file size 571415
SRR5031014 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031014 SRR5031014_1.fastq
Input file:	SRR5031014_1.fastq
trimmed:	SRR5031014-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:06:45 2020 >> started

Wed Mar  4 07:06:47 2020 >> done (1.954s)
3303001 reads processed; of these:
    296 ( 0.01%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
3302697 (99.99%) reads available; of these:
 214792 ( 6.50%) trimmed reads available after processing
3087905 (93.50%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     81	  0.00%
 19	    106	  0.00%
 20	    235	  0.01%
 21	    261	  0.01%
 22	    468	  0.01%
 23	    514	  0.02%
 24	    529	  0.02%
 25	    729	  0.02%
 26	    647	  0.02%
 27	    835	  0.03%
 28	    942	  0.03%
 29	   1657	  0.05%
 30	   1037	  0.03%
 31	   1193	  0.04%
 32	   1343	  0.04%
 33	   1607	  0.05%
 34	   1880	  0.06%
 35	   3344	  0.10%
 36	   2729	  0.08%
 37	   3346	  0.10%
 38	   3199	  0.10%
 39	   3553	  0.11%
 40	   4064	  0.12%
 41	   4336	  0.13%
 42	   5455	  0.17%
 43	   8174	  0.25%
 44	  11716	  0.35%
 45	  14871	  0.45%
 46	  21671	  0.66%
 47	  25757	  0.78%
 48	  38856	  1.18%
 49	  49657	  1.50%
 50	3087905	 93.50%
3302697 reads passed initial QC


criterion=sequence-density
sequence-density=84.74
sequence-density-rank=1
fanout-score=21.54
fanout-score-rank=1
prefix-density=87.15
prefix-fanout=20.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC


criterion=fanout-score
sequence-density=84.74
sequence-density-rank=1
fanout-score=21.54
fanout-score-rank=1
prefix-density=87.15
prefix-fanout=20.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC -o SRR5031014 -
Input file:	STDIN
trimmed:	SRR5031014-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:07:04 2020 >> started

Wed Mar  4 07:07:08 2020 >> done (4.067s)
3224987 reads processed; of these:
 203832 ( 6.32%) short reads filtered out after trimming by size control
  21002 ( 0.65%) empty reads filtered out after trimming by size control
3000153 (93.03%) reads available; of these:
2841963 (94.73%) trimmed reads available after processing
 158190 ( 5.27%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16811	  0.56%
 19	  32693	  1.09%
 20	 183936	  6.13%
 21	 347347	 11.58%
 22	 891851	 29.73%
 23	 402906	 13.43%
 24	 190678	  6.36%
 25	  52634	  1.75%
 26	  28951	  0.96%
 27	  23146	  0.77%
 28	  25480	  0.85%
 29	  19207	  0.64%
 30	  26725	  0.89%
 31	  39565	  1.32%
 32	  38661	  1.29%
 33	  97639	  3.25%
 34	  47009	  1.57%
 35	  41675	  1.39%
 36	  47463	  1.58%
 37	  21592	  0.72%
 38	  24470	  0.82%
 39	  20993	  0.70%
 40	  32080	  1.07%
 41	  38772	  1.29%
 42	  43153	  1.44%
 43	  31666	  1.06%
 44	  23758	  0.79%
 45	  32020	  1.07%
 46	  23528	  0.78%
 47	  16909	  0.56%
 48	   1831	  0.06%
 49	   3358	  0.11%
 50	 131646	  4.39%


criterion=sequence-density
sequence-density=8.30
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.75
sequence-density-rank=20
fanout-score=11.57
fanout-score-rank=1
prefix-density=8.60
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 07:07:31
                             Started mapping on |	Mar 04 07:07:31
                                    Finished on |	Mar 04 07:07:57
       Mapping speed, Million of reads per hour |	426.17

                          Number of input reads |	3077863
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1655200
                        Uniquely mapped reads % |	53.78%
                          Average mapped length |	24.89
                       Number of splices: Total |	1736
            Number of splices: Annotated (sjdb) |	334
                       Number of splices: GT/AG |	1632
                       Number of splices: GC/AG |	20
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	84
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.86
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.14
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	999177
             % of reads mapped to multiple loci |	32.46%
        Number of reads mapped to too many loci |	79523
             % of reads mapped to too many loci |	2.58%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.01%
                     % of reads unmapped: other |	1.16%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	423486	423486	423486
N_multimapping	999177	999177	999177
N_noFeature	80168	103065	1074232
N_ambiguous	918470	404738	15238
UnstrandedReadsAssigned:656562 PositiveStrandReadsAssigned:1147397 NegativeStrandReadsAssigned:565730
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031014 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031014-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,077,863 reads, 790,533 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,185 rounds

 109283 SRR5031014.ke.tsv
  52636 SRR5031014.se.tsv
 161919 total
==> SRR5031014.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031014.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031014 completed mapping pipeline successfully
