Starting /dee2/code/volunteer_pipeline.sh SRR5031092
    current disk space = 160491618304
    free memory = 56718844 
SRR5031092 SRAfilesize
ca5d3ffcd1d08397720dee7c1174076d  SRR5031092.sra
SRR5031092.sra file validated
SRR5031092 is single end
SRR5031092 is conventional basespace
SRR5031092 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.917	31.0	31.0	34.0	30.0	34.0
2	32.348	33.0	31.0	34.0	31.0	34.0
3	32.3235	33.0	31.0	34.0	31.0	34.0
4	36.188	37.0	35.0	37.0	35.0	37.0
5	36.192	37.0	35.0	37.0	35.0	37.0
6	35.96925	37.0	35.0	37.0	35.0	37.0
7	35.81125	37.0	35.0	37.0	35.0	37.0
8	35.61225	37.0	35.0	37.0	33.0	37.0
9	37.039	39.0	37.0	39.0	34.0	39.0
10	36.63325	39.0	37.0	39.0	32.0	39.0
11	37.3485	39.0	37.0	39.0	34.0	39.0
12	37.62275	39.0	37.0	39.0	35.0	39.0
13	37.372	39.0	37.0	39.0	35.0	39.0
14	38.6185	40.0	38.0	41.0	34.0	41.0
15	37.34325	40.0	37.0	41.0	31.0	41.0
16	36.8375	39.0	36.0	40.0	31.0	41.0
17	36.74775	39.0	36.0	41.0	31.0	41.0
18	38.1295	40.0	37.0	41.0	34.0	41.0
19	38.5095	40.0	38.0	41.0	34.0	41.0
20	38.19025	40.0	38.0	41.0	34.0	41.0
21	38.46125	40.0	38.0	41.0	34.0	41.0
22	37.45325	39.0	37.0	40.0	32.0	41.0
23	37.885	39.0	37.0	40.0	34.0	41.0
24	37.26525	39.0	37.0	40.0	31.0	41.0
25	37.986	40.0	37.0	41.0	33.0	41.0
26	37.62175	39.0	37.0	40.0	33.0	41.0
27	37.7625	39.0	37.0	40.0	33.0	41.0
28	38.09175	40.0	38.0	41.0	34.0	41.0
29	38.41	40.0	38.0	41.0	34.0	41.0
30	38.65225	40.0	38.0	41.0	34.0	41.0
31	38.736	40.0	38.0	41.0	35.0	41.0
32	38.6135	40.0	38.0	41.0	34.0	41.0
33	38.69975	40.0	38.0	41.0	35.0	41.0
34	38.32125	40.0	38.0	41.0	34.0	41.0
35	37.586	40.0	37.0	41.0	30.0	41.0
36	33.2315	35.0	30.0	40.0	23.0	41.0
37	34.8045	37.0	33.0	39.0	30.0	40.0
38	36.33075	38.0	35.0	39.0	30.0	40.0
39	36.87075	38.0	36.0	40.0	32.0	40.0
40	37.186	39.0	37.0	40.0	33.0	41.0
41	37.66025	40.0	37.0	40.0	33.0	41.0
42	37.59325	40.0	37.0	41.0	33.0	41.0
43	37.7585	40.0	37.0	41.0	33.0	41.0
44	36.98325	39.0	36.0	40.0	31.0	41.0
45	37.072	39.0	37.0	40.0	31.0	41.0
46	36.911	40.0	36.0	41.0	31.0	41.0
47	35.81325	38.0	35.0	40.0	28.0	41.0
48	36.712	39.0	36.0	40.0	31.0	41.0
49	36.819	39.0	37.0	40.0	31.0	41.0
50	34.9205	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	2.0
16	1.0
17	1.0
18	1.0
19	3.0
20	3.0
21	11.0
22	5.0
23	6.0
24	4.0
25	14.0
26	12.0
27	17.0
28	29.0
29	35.0
30	41.0
31	65.0
32	99.0
33	143.0
34	156.0
35	315.0
36	451.0
37	729.0
38	1154.0
39	702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.501625406351589	25.70642660665166	56.38909727431858	11.40285071267817
2	28.507014028056112	28.0811623246493	21.668336673346694	21.743486973947896
3	16.625	21.15	21.625	40.6
4	29.875	42.15	10.85	17.125
5	23.674999999999997	36.575	29.475	10.274999999999999
6	29.849999999999998	16.225	44.0	9.925
7	11.725	25.324999999999996	22.650000000000002	40.300000000000004
8	24.2	42.625	13.025	20.150000000000002
9	16.25	38.574999999999996	32.800000000000004	12.375
10	17.549999999999997	20.349999999999998	26.5	35.6
11	37.325	7.625	13.775	41.275
12	15.85	16.375	24.825	42.95
13	20.625	18.65	42.675000000000004	18.05
14	43.075	18.975	26.400000000000002	11.55
15	41.75	14.124999999999998	29.975	14.149999999999999
16	12.675	19.025	25.025	43.275000000000006
17	29.875	20.075000000000003	37.225	12.825000000000001
18	33.95	17.4	27.224999999999998	21.425
19	36.3	17.125	33.300000000000004	13.275
20	55.55	15.950000000000001	18.175	10.325
21	17.95	29.225	27.025	25.8
22	32.550000000000004	9.375	49.775000000000006	8.3
23	23.7	11.15	53.225	11.924999999999999
24	52.2	9.15	30.425	8.225
25	59.699999999999996	20.5	13.5	6.3
26	32.550000000000004	49.625	11.025	6.800000000000001
27	13.925	56.10000000000001	22.225	7.75
28	8.9	28.425	52.725	9.950000000000001
29	8.924999999999999	9.575	62.425	19.075
30	7.95	4.025	42.15	45.875
31	12.049999999999999	3.2750000000000004	45.875	38.800000000000004
32	23.799999999999997	4.75	24.85	46.6
33	56.275	5.55	11.225	26.950000000000003
34	73.02499999999999	5.5	9.675	11.799999999999999
35	71.825	5.5	15.875	6.800000000000001
36	47.475	6.2	36.675000000000004	9.65
37	46.425	8.025	23.400000000000002	22.15
38	25.1	11.525	11.15	52.225
39	11.625	21.825	6.625	59.925
40	10.775	51.525	5.4	32.300000000000004
41	21.9	58.175	5.875	14.05
42	51.575	34.35	4.875	9.2
43	60.0	27.250000000000004	5.075	7.675
44	32.1	50.949999999999996	5.675	11.275
45	13.675	57.425000000000004	8.875	20.025000000000002
46	7.3	29.9	17.4	45.4
47	5.375	12.725	38.5	43.4
48	5.0	9.875	24.25	60.875
49	8.975	17.7	10.625	62.7
50	17.849999999999998	38.75	8.85	34.55
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	0.5
30	0.0
31	0.0
32	0.0
33	1.5
34	3.0
35	5.5
36	8.0
37	10.0
38	12.0
39	13.5
40	15.0
41	19.5
42	24.0
43	84.5
44	145.0
45	198.5
46	252.0
47	410.5
48	569.0
49	591.5
50	614.0
51	832.5
52	1051.0
53	706.0
54	361.0
55	345.5
56	330.0
57	314.0
58	298.0
59	210.0
60	122.0
61	95.0
62	68.0
63	54.0
64	40.0
65	30.0
66	20.0
67	16.5
68	13.0
69	12.5
70	12.0
71	24.0
72	36.0
73	19.5
74	3.0
75	2.0
76	1.0
77	1.0
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.2
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.19047619047619	28.799999999999997
2	9.25925925925926	7.000000000000001
3	4.232804232804233	4.8
4	2.380952380952381	3.5999999999999996
5	0.992063492063492	1.875
6	1.1904761904761905	2.7
7	0.9259259259259258	2.45
8	0.4629629629629629	1.4000000000000001
9	0.3306878306878307	1.125
>10	3.7037037037037033	27.224999999999998
>50	0.13227513227513227	4.075
>100	0.1984126984126984	14.95
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	257	6.425	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	210	5.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	131	3.2750000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	97	2.4250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	45	1.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC	27	0.675	RNA PCR Primer, Index 5 (97% over 35bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	23	0.575	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC	21	0.525	RNA PCR Primer, Index 16 (100% over 50bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG	15	0.375	RNA PCR Primer, Index 7 (97% over 36bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (96% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	13	0.325	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT	11	0.27499999999999997	RNA PCR Primer, Index 16 (100% over 37bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTACCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC	6	0.15	RNA PCR Primer, Index 5 (97% over 35bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC	6	0.15	RNA PCR Primer, Index 16 (100% over 44bp)
TGAGGTAGTAGATTGTCTAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCGTTCGCGCTCTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTAT	6	0.15	RNA PCR Primer, Index 16 (100% over 48bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CTGGCCCTCTCTGCCCTTCCGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG	5	0.125	RNA PCR Primer, Index 16 (100% over 49bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACCCGTCCATCTCGTATGC	5	0.125	RNA PCR Primer, Index 16 (98% over 50bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTCTGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.75	0.0	0.0	0.0
2	0.0	0.95	0.0	0.0	0.0
3	0.0	1.175	0.0	0.0	0.0
4	0.0	1.2	0.0	0.0	0.0
5	0.0	1.3	0.0	0.0	0.0
6	0.0	1.5	0.0	0.0	0.0
7	0.0	1.8	0.0	0.0	0.0
8	0.0	2.0	0.0	0.0	0.0
9	0.0	2.1	0.0	0.0	0.0
10	0.0	2.325	0.0	0.0	0.0
11	0.0	2.525	0.0	0.0	0.0
12	0.0	2.7	0.0	0.0	0.0
13	0.0	2.925	0.0	0.0	0.0
14	0.0	3.4	0.0	0.0	0.0
15	0.0	4.45	0.0	0.0	0.0
16	0.0	5.725	0.0	0.0	0.0
17	0.0	6.7	0.0	0.0	0.0
18	0.0	7.7	0.0	0.0	0.0
19	0.0	8.275	0.0	0.0	0.0
20	0.0	9.35	0.0	0.0	0.0
21	0.0	13.1	0.0	0.0	0.0
22	0.0	25.55	0.0	0.0	0.0
23	0.0	59.525	0.0	0.0	0.0
24	0.0	78.675	0.0	0.0	0.0
25	0.0	84.125	0.0	0.0	0.0
26	0.0	85.15	0.0	0.0	0.0
27	0.0	85.575	0.0	0.0	0.0
28	0.0	86.025	0.0	0.0	0.0
29	0.0	86.425	0.0	0.0	0.0
30	0.0	86.95	0.0	0.0	0.0
31	0.0	87.525	0.0	0.0	0.0
32	0.0	88.3	0.0	0.0	0.0
33	0.0	89.45	0.0	0.0	0.0
34	0.0	90.4	0.0	0.0	0.0
35	0.0	90.975	0.0	0.0	0.0
36	0.0	91.275	0.0	0.0	0.0
37	0.0	91.425	0.0	0.0	0.0
38	0.0	91.75	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
AACGCTG	45	2.728484E-10	44.000004	8
GGTTGTA	20	6.952051E-4	44.000004	11
AAGTAAT	40	5.315087E-9	44.000004	4
TCAAGTA	40	5.315087E-9	44.000004	2
ACGCTGT	45	2.728484E-10	44.000004	9
AATCCAG	40	5.315087E-9	44.000004	8
CTGAGAC	20	6.952051E-4	44.000004	4
TAAACAT	45	2.728484E-10	44.000004	3
ATCCAGG	40	5.315087E-9	44.000004	9
AAACATC	45	2.728484E-10	44.000004	4
TTCAAGT	40	5.315087E-9	44.000004	1
TTCAACG	45	2.728484E-10	44.000004	5
TCGGTGA	45	2.728484E-10	44.000004	15
CCTGAGA	20	6.952051E-4	44.000004	3
CATTCAA	45	2.728484E-10	44.000004	3
TCAACGC	45	2.728484E-10	44.000004	6
GATTGTA	20	6.952051E-4	44.000004	11
AGTAATC	40	5.315087E-9	44.000004	5
ATTGTAT	20	6.952051E-4	44.000004	12
>>END_MODULE
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169820 spots for SRR5031092.sra
Written 169820 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
Read 169810 spots for SRR5031092.sra
Written 169810 spots for SRR5031092.sra
SRR ids: ['SRR5031092.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_j4ckrryy
SRR5031092.sra spots: 2037730
blocks: [[1, 169810], [169811, 339620], [339621, 509430], [509431, 679240], [679241, 849050], [849051, 1018860], [1018861, 1188670], [1188671, 1358480], [1358481, 1528290], [1528291, 1698100], [1698101, 1867910], [1867911, 2037730]]
SRR5031092 file size 352116
SRR5031092 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031092 SRR5031092_1.fastq
Input file:	SRR5031092_1.fastq
trimmed:	SRR5031092-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:06 2020 >> started

Wed Mar  4 07:22:08 2020 >> done (1.459s)
2037730 reads processed; of these:
    161 ( 0.01%) short reads filtered out after trimming by size control
      7 ( 0.00%) empty reads filtered out after trimming by size control
2037562 (99.99%) reads available; of these:
 171661 ( 8.42%) trimmed reads available after processing
1865901 (91.58%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     45	  0.00%
 19	     63	  0.00%
 20	    118	  0.01%
 21	    181	  0.01%
 22	    363	  0.02%
 23	    386	  0.02%
 24	    351	  0.02%
 25	    519	  0.03%
 26	    323	  0.02%
 27	    483	  0.02%
 28	    528	  0.03%
 29	   1137	  0.06%
 30	    548	  0.03%
 31	    536	  0.03%
 32	    622	  0.03%
 33	   1263	  0.06%
 34	   1961	  0.10%
 35	   5737	  0.28%
 36	   3126	  0.15%
 37	   2793	  0.14%
 38	   2538	  0.12%
 39	   2983	  0.15%
 40	   3616	  0.18%
 41	   3941	  0.19%
 42	   4577	  0.22%
 43	   7422	  0.36%
 44	   8217	  0.40%
 45	  13086	  0.64%
 46	  13138	  0.64%
 47	  14131	  0.69%
 48	  38039	  1.87%
 49	  38890	  1.91%
 50	1865901	 91.58%
2037562 reads passed initial QC


criterion=sequence-density
sequence-density=91.91
sequence-density-rank=1
fanout-score=16.29
fanout-score-rank=2
prefix-density=92.13
prefix-fanout=16.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC


criterion=fanout-score
sequence-density=3.70
sequence-density-rank=4
fanout-score=68.55
fanout-score-rank=1
prefix-density=92.36
prefix-fanout=2.7
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC -o SRR5031092 -
Input file:	STDIN
trimmed:	SRR5031092-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:21 2020 >> started

Wed Mar  4 07:22:24 2020 >> done (3.292s)
1993267 reads processed; of these:
 153312 ( 7.69%) short reads filtered out after trimming by size control
  18907 ( 0.95%) empty reads filtered out after trimming by size control
1821048 (91.36%) reads available; of these:
1777834 (97.63%) trimmed reads available after processing
  43214 ( 2.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12324	  0.68%
 19	  27034	  1.48%
 20	  95150	  5.23%
 21	 243243	 13.36%
 22	 682660	 37.49%
 23	 395216	 21.70%
 24	 130705	  7.18%
 25	  19213	  1.06%
 26	   9965	  0.55%
 27	   9733	  0.53%
 28	  10186	  0.56%
 29	   8067	  0.44%
 30	  12393	  0.68%
 31	  15270	  0.84%
 32	  14678	  0.81%
 33	  21472	  1.18%
 34	  10973	  0.60%
 35	   6870	  0.38%
 36	   7288	  0.40%
 37	   4769	  0.26%
 38	   4376	  0.24%
 39	   4029	  0.22%
 40	   4691	  0.26%
 41	   6546	  0.36%
 42	   6577	  0.36%
 43	   5389	  0.30%
 44	   4303	  0.24%
 45	   5713	  0.31%
 46	   4409	  0.24%
 47	   3088	  0.17%
 48	    493	  0.03%
 49	    539	  0.03%
 50	  33686	  1.85%


criterion=sequence-density
sequence-density=4.64
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=TGTAAACATCCTCGACTGGAAGC


criterion=fanout-score
sequence-density=2.27
sequence-density-rank=7
fanout-score=16.12
fanout-score-rank=1
prefix-density=2.28
prefix-fanout=16.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC
                                 Started job on |	Mar 04 07:23:13
                             Started mapping on |	Mar 04 07:23:13
                                    Finished on |	Mar 04 07:23:39
       Mapping speed, Million of reads per hour |	258.28

                          Number of input reads |	1865343
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	796307
                        Uniquely mapped reads % |	42.69%
                          Average mapped length |	23.05
                       Number of splices: Total |	2818
            Number of splices: Annotated (sjdb) |	510
                       Number of splices: GT/AG |	2756
                       Number of splices: GC/AG |	25
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	37
                      Mismatch rate per base, % |	0.33%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.42
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	922904
             % of reads mapped to multiple loci |	49.48%
        Number of reads mapped to too many loci |	27478
             % of reads mapped to too many loci |	1.47%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.67%
                     % of reads unmapped: other |	0.69%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	146132	146132	146132
N_multimapping	922904	922904	922904
N_noFeature	47866	53872	607479
N_ambiguous	403143	236500	3766
UnstrandedReadsAssigned:345298 PositiveStrandReadsAssigned:505935 NegativeStrandReadsAssigned:185062
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031092 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031092-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,865,343 reads, 289,059 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,010 rounds

 109283 SRR5031092.ke.tsv
  52636 SRR5031092.se.tsv
 161919 total
==> SRR5031092.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031092.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031092 completed mapping pipeline successfully
