Starting /dee2/code/volunteer_pipeline.sh SRR5031002
    current disk space = 160560300032
    free memory = 73949032 
SRR5031002 SRAfilesize
0780da60f974005c5dffbef64569750c  SRR5031002.sra
SRR5031002.sra file validated
SRR5031002 is single end
SRR5031002 is conventional basespace
SRR5031002 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031002_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.248	33.0	31.0	34.0	30.0	34.0
2	32.46875	34.0	31.0	34.0	31.0	34.0
3	32.53575	34.0	31.0	34.0	31.0	34.0
4	36.164	37.0	35.0	37.0	35.0	37.0
5	36.04275	37.0	35.0	37.0	35.0	37.0
6	35.9815	37.0	35.0	37.0	35.0	37.0
7	35.9125	37.0	35.0	37.0	35.0	37.0
8	35.9065	37.0	35.0	37.0	35.0	37.0
9	37.54	39.0	37.0	39.0	35.0	39.0
10	37.43975	39.0	37.0	39.0	34.0	39.0
11	36.9575	39.0	37.0	39.0	33.0	39.0
12	37.443	39.0	37.0	39.0	34.0	39.0
13	37.50525	39.0	37.0	39.0	35.0	39.0
14	38.6005	40.0	38.0	41.0	34.0	41.0
15	38.71525	40.0	38.0	41.0	35.0	41.0
16	38.751	40.0	38.0	41.0	34.0	41.0
17	38.9235	40.0	38.0	41.0	35.0	41.0
18	38.77375	40.0	38.0	41.0	34.0	41.0
19	38.73825	40.0	38.0	41.0	34.0	41.0
20	38.556	40.0	38.0	41.0	34.0	41.0
21	38.7395	40.0	38.0	41.0	34.0	41.0
22	38.59025	40.0	38.0	41.0	34.0	41.0
23	38.3055	40.0	38.0	41.0	33.0	41.0
24	38.684	40.0	38.0	41.0	34.0	41.0
25	38.8315	40.0	38.0	41.0	34.0	41.0
26	38.611	40.0	38.0	41.0	34.0	41.0
27	38.77325	40.0	38.0	41.0	34.0	41.0
28	38.439	40.0	38.0	41.0	34.0	41.0
29	38.1905	40.0	38.0	41.0	33.0	41.0
30	37.9555	40.0	38.0	41.0	33.0	41.0
31	38.3755	40.0	38.0	41.0	33.0	41.0
32	38.40275	40.0	38.0	41.0	34.0	41.0
33	38.05625	40.0	38.0	41.0	33.0	41.0
34	37.98225	40.0	38.0	41.0	33.0	41.0
35	37.8045	40.0	37.0	41.0	33.0	41.0
36	37.6315	40.0	37.0	41.0	32.0	41.0
37	36.89425	39.0	36.0	41.0	30.0	41.0
38	37.069	39.0	36.0	40.0	31.0	41.0
39	37.54175	39.0	37.0	41.0	32.0	41.0
40	37.62975	40.0	37.0	41.0	33.0	41.0
41	37.59425	40.0	37.0	41.0	33.0	41.0
42	37.23175	40.0	36.0	41.0	31.0	41.0
43	37.31375	40.0	37.0	41.0	32.0	41.0
44	37.48775	40.0	37.0	41.0	33.0	41.0
45	37.46	40.0	37.0	41.0	33.0	41.0
46	37.065	40.0	37.0	41.0	31.0	41.0
47	36.42625	39.0	35.0	41.0	30.0	41.0
48	36.198	39.0	35.0	41.0	29.0	41.0
49	36.26875	39.0	35.0	41.0	30.0	41.0
50	35.63225	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	1.0
16	3.0
17	2.0
18	3.0
19	1.0
20	3.0
21	1.0
22	7.0
23	8.0
24	12.0
25	9.0
26	20.0
27	16.0
28	24.0
29	43.0
30	66.0
31	70.0
32	88.0
33	117.0
34	166.0
35	224.0
36	292.0
37	451.0
38	798.0
39	1573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.6	23.549999999999997	47.775	16.075
2	24.560963371801304	26.091319618665327	20.321123933768188	29.026593075765177
3	27.35	17.150000000000002	22.7	32.800000000000004
4	32.9	29.675	16.25	21.175
5	21.3	39.125	24.825	14.75
6	28.125	16.075	39.5	16.3
7	19.425	19.775000000000002	23.75	37.05
8	21.15	26.125	18.925	33.800000000000004
9	24.55	29.7	29.225	16.525000000000002
10	23.65	18.675	29.049999999999997	28.625
11	33.125	14.85	16.2	35.825
12	19.425	19.45	31.775	29.349999999999998
13	31.724999999999998	16.55	29.375	22.35
14	27.05	31.125000000000004	22.6	19.225
15	38.95	21.0	23.775	16.275000000000002
16	18.625	20.125	23.9	37.35
17	26.275	21.349999999999998	34.35	18.025
18	24.025	19.875	31.15	24.95
19	35.475	16.325	30.65	17.549999999999997
20	44.175	16.3	24.55	14.975
21	26.450000000000003	21.7	22.5	29.349999999999998
22	26.825	18.2	43.75	11.225
23	24.625	17.8	41.0	16.575
24	46.625	16.575	24.275	12.525
25	45.425	22.625	19.775000000000002	12.174999999999999
26	26.700000000000003	41.225	15.325	16.75
27	16.7	42.275	23.925	17.1
28	13.325000000000001	25.7	43.45	17.525
29	16.05	13.55	50.575	19.825
30	11.3	8.325000000000001	35.525	44.85
31	15.575	5.675	44.800000000000004	33.95
32	26.700000000000003	10.424999999999999	20.974999999999998	41.9
33	50.025	9.375	14.95	25.650000000000002
34	55.025	8.525	17.549999999999997	18.9
35	63.224999999999994	8.375	17.45	10.95
36	38.324999999999996	9.525	38.224999999999994	13.925
37	42.625	15.075	20.05	22.25
38	22.3	17.424999999999997	13.875000000000002	46.400000000000006
39	20.4	22.425	12.3	44.875
40	16.150000000000002	45.574999999999996	13.600000000000001	24.675
41	24.375	43.525000000000006	14.025000000000002	18.075
42	44.574999999999996	26.700000000000003	14.85	13.875000000000002
43	47.3	26.5	13.700000000000001	12.5
44	30.95	42.425000000000004	8.975	17.65
45	25.124999999999996	43.95	13.200000000000001	17.724999999999998
46	16.225	24.45	20.125	39.2
47	11.35	18.625	37.475	32.550000000000004
48	13.850000000000001	13.475000000000001	22.650000000000002	50.025
49	17.75	17.974999999999998	14.649999999999999	49.625
50	18.525	34.25	13.725000000000001	33.5
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	1.0
25	1.0
26	1.0
27	3.0
28	5.0
29	4.0
30	3.0
31	10.0
32	17.0
33	15.0
34	13.0
35	21.5
36	30.0
37	37.5
38	45.0
39	47.5
40	50.0
41	54.0
42	58.0
43	75.0
44	92.0
45	124.5
46	157.0
47	279.5
48	402.0
49	478.0
50	554.0
51	673.0
52	792.0
53	659.0
54	526.0
55	524.5
56	523.0
57	414.0
58	305.0
59	204.0
60	103.0
61	74.0
62	45.0
63	80.5
64	116.0
65	79.0
66	42.0
67	30.0
68	18.0
69	22.0
70	26.0
71	43.5
72	61.0
73	34.5
74	8.0
75	5.0
76	2.0
77	2.5
78	3.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.35000000000000003
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.05
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00487210718636	31.2
2	10.23142509135201	8.4
3	3.7758830694275276	4.65
4	2.0097442143727164	3.3000000000000003
5	1.9488428745432398	4.0
6	0.9744214372716199	2.4
7	0.6090133982947624	1.7500000000000002
8	0.7917174177831912	2.6
9	0.48721071863580995	1.7999999999999998
>10	2.6187576126674785	19.15
>50	0.3654080389768575	11.825
>100	0.18270401948842874	8.924999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	134	3.35	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGC	119	2.9749999999999996	RNA PCR Primer, Index 22 (100% over 50bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	104	2.6	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	94	2.35	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	89	2.225	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	82	2.0500000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	76	1.9	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	67	1.675	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	65	1.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	48	1.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	47	1.175	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	28	0.7000000000000001	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	22	0.5499999999999999	RNA PCR Primer, Index 2 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21	0.525	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC	18	0.44999999999999996	RNA PCR Primer, Index 22 (100% over 44bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	14	0.35000000000000003	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	11	0.27499999999999997	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	11	0.27499999999999997	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	10	0.25	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	10	0.25	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	10	0.25	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	10	0.25	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	8	0.2	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	8	0.2	RNA PCR Primer, Index 2 (100% over 34bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTAT	8	0.2	RNA PCR Primer, Index 22 (100% over 48bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	7	0.17500000000000002	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	7	0.17500000000000002	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATATCGTATGC	6	0.15	RNA PCR Primer, Index 22 (98% over 50bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	6	0.15	No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	5	0.125	No Hit
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTGGAATTC	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CTTCTCACTACTGCACTTGACTAGTCTTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	5	0.125	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	5	0.125	No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	5	0.125	No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	3.425	0.0	0.0	0.0
2	0.0	3.475	0.0	0.0	0.0
3	0.0	3.675	0.0	0.0	0.0
4	0.0	3.675	0.0	0.0	0.0
5	0.0	3.725	0.0	0.0	0.0
6	0.0	3.75	0.0	0.0	0.0
7	0.0	4.4	0.0	0.0	0.0
8	0.0	4.625	0.0	0.0	0.0
9	0.0	4.7	0.0	0.0	0.0
10	0.0	4.775	0.0	0.0	0.0
11	0.0	4.975	0.0	0.0	0.0
12	0.0	5.175	0.0	0.0	0.0
13	0.0	5.4	0.0	0.0	0.0
14	0.0	5.575	0.0	0.0	0.0
15	0.0	5.9	0.0	0.0	0.0
16	0.0	6.875	0.0	0.0	0.0
17	0.0	7.575	0.0	0.0	0.0
18	0.0	8.65	0.0	0.0	0.0
19	0.0	9.075	0.0	0.0	0.0
20	0.0	10.1	0.0	0.0	0.0
21	0.0	15.025	0.0	0.0	0.0
22	0.0	24.15	0.0	0.0	0.0
23	0.0	50.025	0.0	0.0	0.0
24	0.0	60.675	0.0	0.0	0.0
25	0.0	66.25	0.0	0.0	0.0
26	0.0	67.8	0.0	0.0	0.0
27	0.0	68.45	0.0	0.0	0.0
28	0.0	69.2	0.0	0.0	0.0
29	0.0	70.1	0.0	0.0	0.0
30	0.0	70.7	0.0	0.0	0.0
31	0.0	72.025	0.0	0.0	0.0
32	0.0	73.4	0.0	0.0	0.0
33	0.0	74.9	0.0	0.0	0.0
34	0.0	78.8	0.0	0.0	0.0
35	0.0	80.55	0.0	0.0	0.0
36	0.0	81.8	0.0	0.0	0.0
37	0.0	83.05	0.0	0.0	0.0
38	0.0	84.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGGA	20	6.952051E-4	44.000004	20
ATCTCGT	20	6.952051E-4	44.000004	40
TCTCGTA	20	6.952051E-4	44.000004	41
GATCTCG	20	6.952051E-4	44.000004	39
TACGATC	20	6.952051E-4	44.000004	36
CGTACGA	20	6.952051E-4	44.000004	34
CGTATGC	20	6.952051E-4	44.000004	44
TCGTATG	20	6.952051E-4	44.000004	43
ACGATCT	20	6.952051E-4	44.000004	37
CTGTTGG	20	6.952051E-4	44.000004	19
CTCGTAT	20	6.952051E-4	44.000004	42
CGATCTC	20	6.952051E-4	44.000004	38
GTACGAT	20	6.952051E-4	44.000004	35
AACGCTG	25	3.6958358E-5	44.0	8
GGTGAGT	25	3.6958358E-5	44.0	17
ACGCTGT	25	3.6958358E-5	44.0	9
CTGAGCT	30	1.9515464E-6	44.0	11
TTCAACG	25	3.6958358E-5	44.0	5
TCGGTGA	25	3.6958358E-5	44.0	15
GAGCTTG	30	1.9515464E-6	44.0	13
>>END_MODULE
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277952 spots for SRR5031002.sra
Written 277952 spots for SRR5031002.sra
Read 277956 spots for SRR5031002.sra
Written 277956 spots for SRR5031002.sra
SRR ids: ['SRR5031002.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_q2a5xnow
SRR5031002.sra spots: 3335428
blocks: [[1, 277952], [277953, 555904], [555905, 833856], [833857, 1111808], [1111809, 1389760], [1389761, 1667712], [1667713, 1945664], [1945665, 2223616], [2223617, 2501568], [2501569, 2779520], [2779521, 3057472], [3057473, 3335428]]
SRR5031002 file size 577038
SRR5031002 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031002 SRR5031002_1.fastq
Input file:	SRR5031002_1.fastq
trimmed:	SRR5031002-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:03:36 2020 >> started

Wed Mar  4 07:03:38 2020 >> done (1.542s)
3335428 reads processed; of these:
    366 ( 0.01%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
3335054 (99.99%) reads available; of these:
 226309 ( 6.79%) trimmed reads available after processing
3108745 (93.21%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     95	  0.00%
 19	    104	  0.00%
 20	    230	  0.01%
 21	    235	  0.01%
 22	    430	  0.01%
 23	    503	  0.02%
 24	    499	  0.01%
 25	    745	  0.02%
 26	    644	  0.02%
 27	    757	  0.02%
 28	    861	  0.03%
 29	   1294	  0.04%
 30	    998	  0.03%
 31	   1040	  0.03%
 32	   1187	  0.04%
 33	   1490	  0.04%
 34	   1739	  0.05%
 35	   3277	  0.10%
 36	   2915	  0.09%
 37	   3568	  0.11%
 38	   3711	  0.11%
 39	   4613	  0.14%
 40	   6808	  0.20%
 41	   5014	  0.15%
 42	   6055	  0.18%
 43	   8552	  0.26%
 44	  12239	  0.37%
 45	  16276	  0.49%
 46	  22892	  0.69%
 47	  28140	  0.84%
 48	  40448	  1.21%
 49	  48950	  1.47%
 50	3108745	 93.21%
3335054 reads passed initial QC


criterion=sequence-density
sequence-density=84.14
sequence-density-rank=1
fanout-score=21.32
fanout-score-rank=1
prefix-density=83.97
prefix-fanout=21.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC


criterion=fanout-score
sequence-density=84.14
sequence-density-rank=1
fanout-score=21.32
fanout-score-rank=1
prefix-density=83.97
prefix-fanout=21.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC -o SRR5031002 -
Input file:	STDIN
trimmed:	SRR5031002-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:03:53 2020 >> started

Wed Mar  4 07:03:57 2020 >> done (3.404s)
3256582 reads processed; of these:
 196350 ( 6.03%) short reads filtered out after trimming by size control
 105635 ( 3.24%) empty reads filtered out after trimming by size control
2954597 (90.73%) reads available; of these:
2785639 (94.28%) trimmed reads available after processing
 168958 ( 5.72%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16548	  0.56%
 19	  29799	  1.01%
 20	 166211	  5.63%
 21	 319595	 10.82%
 22	 849746	 28.76%
 23	 365051	 12.36%
 24	 184852	  6.26%
 25	  54274	  1.84%
 26	  27951	  0.95%
 27	  23979	  0.81%
 28	  26980	  0.91%
 29	  21021	  0.71%
 30	  34622	  1.17%
 31	  51465	  1.74%
 32	  51526	  1.74%
 33	 109548	  3.71%
 34	  51467	  1.74%
 35	  42088	  1.42%
 36	  47844	  1.62%
 37	  23567	  0.80%
 38	  25214	  0.85%
 39	  23032	  0.78%
 40	  36014	  1.22%
 41	  42038	  1.42%
 42	  47204	  1.60%
 43	  32418	  1.10%
 44	  25258	  0.85%
 45	  34576	  1.17%
 46	  25236	  0.85%
 47	  17791	  0.60%
 48	   2009	  0.07%
 49	   4222	  0.14%
 50	 141451	  4.79%


criterion=sequence-density
sequence-density=6.49
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=30
fanout-score=16.08
fanout-score-rank=1
prefix-density=8.66
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 07:04:17
                             Started mapping on |	Mar 04 07:04:18
                                    Finished on |	Mar 04 07:04:41
       Mapping speed, Million of reads per hour |	474.74

                          Number of input reads |	3033069
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1680539
                        Uniquely mapped reads % |	55.41%
                          Average mapped length |	25.56
                       Number of splices: Total |	1932
            Number of splices: Annotated (sjdb) |	584
                       Number of splices: GT/AG |	1800
                       Number of splices: GC/AG |	27
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	104
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.67
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	919725
             % of reads mapped to multiple loci |	30.32%
        Number of reads mapped to too many loci |	89862
             % of reads mapped to too many loci |	2.96%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.25%
                     % of reads unmapped: other |	1.06%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	432805	432805	432805
N_multimapping	919725	919725	919725
N_noFeature	90145	115701	1125332
N_ambiguous	914044	429910	15389
UnstrandedReadsAssigned:676350 PositiveStrandReadsAssigned:1134928 NegativeStrandReadsAssigned:539818
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031002 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031002-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,033,069 reads, 780,190 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,181 rounds

 109283 SRR5031002.ke.tsv
  52636 SRR5031002.se.tsv
 161919 total
==> SRR5031002.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031002.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031002 completed mapping pipeline successfully
