Starting /dee2/code/volunteer_pipeline.sh SRR5030925
    current disk space = 160627097600
    free memory = 73948100 
SRR5030925 SRAfilesize
7e7e8ef245ce7b7badb3fa2482306087  SRR5030925.sra
SRR5030925.sra file validated
SRR5030925 is single end
SRR5030925 is conventional basespace
SRR5030925 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.386	34.0	31.0	34.0	31.0	34.0
2	32.716	34.0	31.0	34.0	31.0	34.0
3	32.68175	34.0	31.0	34.0	31.0	34.0
4	36.2565	37.0	37.0	37.0	35.0	37.0
5	36.2945	37.0	37.0	37.0	35.0	37.0
6	36.06725	37.0	35.0	37.0	35.0	37.0
7	36.114	37.0	35.0	37.0	35.0	37.0
8	36.1985	37.0	36.0	37.0	35.0	37.0
9	37.922	39.0	38.0	39.0	35.0	39.0
10	37.839	39.0	38.0	39.0	35.0	39.0
11	37.9025	39.0	38.0	39.0	35.0	39.0
12	37.68875	39.0	38.0	39.0	35.0	39.0
13	37.7485	39.0	38.0	39.0	35.0	39.0
14	39.311	41.0	39.0	41.0	36.0	41.0
15	39.3035	41.0	39.0	41.0	36.0	41.0
16	39.1375	40.0	39.0	41.0	36.0	41.0
17	39.17225	40.0	39.0	41.0	36.0	41.0
18	39.3495	41.0	39.0	41.0	36.0	41.0
19	39.17625	40.0	39.0	41.0	36.0	41.0
20	38.99775	40.0	39.0	41.0	35.0	41.0
21	39.1065	41.0	39.0	41.0	36.0	41.0
22	39.3735	41.0	39.0	41.0	36.0	41.0
23	39.26175	41.0	39.0	41.0	36.0	41.0
24	38.72175	40.0	38.0	41.0	34.0	41.0
25	38.67675	40.0	39.0	41.0	34.0	41.0
26	38.97575	40.0	39.0	41.0	35.0	41.0
27	39.06275	41.0	39.0	41.0	35.0	41.0
28	38.92725	40.0	39.0	41.0	35.0	41.0
29	38.36725	40.0	38.0	41.0	33.0	41.0
30	38.587	40.0	38.0	41.0	34.0	41.0
31	38.876	40.0	38.0	41.0	35.0	41.0
32	38.9435	40.0	39.0	41.0	35.0	41.0
33	37.7075	40.0	38.0	41.0	32.0	41.0
34	37.993	40.0	38.0	41.0	33.0	41.0
35	37.61175	40.0	37.0	41.0	33.0	41.0
36	32.74425	38.0	30.0	40.0	16.0	41.0
37	35.277	38.0	33.0	40.0	28.0	41.0
38	36.98025	39.0	37.0	40.0	32.0	41.0
39	37.35075	39.0	37.0	40.0	32.0	41.0
40	37.4875	40.0	37.0	41.0	33.0	41.0
41	37.05475	39.0	36.0	40.0	31.0	41.0
42	37.14575	39.0	37.0	41.0	31.0	41.0
43	37.05625	39.0	37.0	41.0	31.0	41.0
44	37.48375	40.0	37.0	41.0	33.0	41.0
45	37.11	40.0	37.0	41.0	32.0	41.0
46	34.98675	38.0	34.0	40.0	26.0	41.0
47	35.7825	39.0	35.0	40.0	28.0	41.0
48	35.8635	39.0	35.0	40.0	29.0	41.0
49	34.86175	38.0	34.0	40.0	27.0	41.0
50	31.01025	34.0	28.0	39.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	0.0
15	0.0
16	1.0
17	2.0
18	2.0
19	5.0
20	6.0
21	3.0
22	4.0
23	9.0
24	10.0
25	15.0
26	23.0
27	28.0
28	28.0
29	38.0
30	64.0
31	54.0
32	80.0
33	111.0
34	139.0
35	209.0
36	303.0
37	541.0
38	1046.0
39	1277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.41265060240964	21.159638554216865	53.21285140562249	16.214859437751002
2	23.425	27.150000000000002	21.625	27.800000000000004
3	18.975	19.825	19.525000000000002	41.675000000000004
4	25.575	34.9	14.2	25.324999999999996
5	22.15	38.75	26.325	12.775
6	36.25	15.425	34.575	13.750000000000002
7	16.3	24.4	25.25	34.050000000000004
8	24.625	40.1	15.299999999999999	19.975
9	19.625	37.625	30.099999999999998	12.65
10	21.5	20.7	27.05	30.75
11	30.325000000000003	14.249999999999998	14.725	40.699999999999996
12	16.625	17.95	24.875	40.550000000000004
13	19.05	23.400000000000002	36.1	21.45
14	34.75	25.35	24.8	15.1
15	36.875	24.775	26.325	12.025
16	14.799999999999999	28.625	20.474999999999998	36.1
17	22.85	24.925	37.475	14.75
18	27.650000000000002	19.2	29.25	23.9
19	38.725	14.05	28.975	18.25
20	46.325	17.575	24.349999999999998	11.75
21	22.900000000000002	23.474999999999998	24.175	29.45
22	32.074999999999996	12.75	46.225	8.95
23	26.474999999999998	12.725	46.85	13.950000000000001
24	53.05	13.125	25.95	7.875
25	53.0	23.5	13.5	10.0
26	27.400000000000002	47.975	13.525	11.1
27	13.875000000000002	50.375	23.425	12.325
28	10.45	24.3	50.449999999999996	14.799999999999999
29	11.0	11.799999999999999	57.825	19.375
30	11.275	6.5	36.625	45.6
31	13.850000000000001	6.6000000000000005	45.775	33.775
32	28.050000000000004	6.75	22.0	43.2
33	54.675	9.225	13.4	22.7
34	64.5	7.725	14.45	13.325000000000001
35	63.525	7.75	18.3	10.424999999999999
36	45.35	7.425	34.825	12.4
37	45.25	10.725	21.224999999999998	22.8
38	23.45	14.7	11.75	50.1
39	14.374999999999998	24.075	9.775	51.775000000000006
40	14.174999999999999	49.7	11.425	24.7
41	24.275	51.849999999999994	9.125	14.75
42	52.225	28.9	10.85	8.025
43	53.55	27.825	8.95	9.675
44	29.475	49.7	8.475000000000001	12.35
45	16.575	53.5	11.600000000000001	18.325
46	12.625	26.700000000000003	18.9	41.775
47	9.3	14.274999999999999	41.075	35.35
48	11.575000000000001	11.475	21.025	55.925000000000004
49	14.05	20.424999999999997	12.425	53.1
50	19.650000000000002	37.125	12.0	31.225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	2.0
30	2.0
31	6.0
32	10.0
33	16.0
34	22.0
35	29.5
36	37.0
37	48.5
38	60.0
39	54.0
40	48.0
41	51.0
42	54.0
43	94.5
44	135.0
45	187.0
46	239.0
47	374.5
48	510.0
49	551.5
50	593.0
51	744.5
52	896.0
53	636.0
54	376.0
55	389.0
56	402.0
57	353.0
58	304.0
59	192.0
60	80.0
61	66.0
62	52.0
63	70.5
64	89.0
65	62.5
66	36.0
67	22.5
68	9.0
69	8.0
70	7.0
71	11.0
72	15.0
73	13.0
74	11.0
75	7.0
76	3.0
77	2.5
78	2.0
79	1.5
80	1.0
81	2.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43784786641929	30.9
2	8.410636982065553	6.800000000000001
3	4.5145330859616575	5.475
4	2.1645021645021645	3.5000000000000004
5	1.7934446505875077	3.6249999999999996
6	1.051329622758194	2.55
7	0.8658008658008658	2.45
8	0.3710575139146568	1.2
9	0.5565862708719851	2.025
>10	3.4013605442176873	24.125
>50	0.24737167594310452	6.550000000000001
>100	0.1855287569573284	10.8
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	182	4.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	126	3.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	124	3.1	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	90	2.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	64	1.6	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	57	1.425	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	42	1.05	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	38	0.95	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	18	0.44999999999999996	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	18	0.44999999999999996	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	15	0.375	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	13	0.325	No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	12	0.3	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC	11	0.27499999999999997	RNA PCR Primer, Index 5 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	10	0.25	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGAAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC	8	0.2	RNA PCR Primer, Index 6 (100% over 44bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	6	0.15	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	6	0.15	RNA PCR Primer, Index 6 (100% over 50bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCAGGCTCAGTCCCCTCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
ACAAATGATGAACCTTTTGACGGGCGGAAAGAAACTCTGTGCTGAATGTC	5	0.125	No Hit
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTTGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	5	0.125	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.15	0.0	0.0	0.0
2	0.0	0.15	0.0	0.0	0.0
3	0.0	0.225	0.0	0.0	0.0
4	0.0	0.25	0.0	0.0	0.0
5	0.0	0.325	0.0	0.0	0.0
6	0.0	0.35	0.0	0.0	0.0
7	0.0	0.575	0.0	0.0	0.0
8	0.0	0.65	0.0	0.0	0.0
9	0.0	0.7	0.0	0.0	0.0
10	0.0	0.85	0.0	0.0	0.0
11	0.0	0.975	0.0	0.0	0.0
12	0.0	1.225	0.0	0.0	0.0
13	0.0	1.525	0.0	0.0	0.0
14	0.0	1.8	0.0	0.0	0.0
15	0.0	2.05	0.0	0.0	0.0
16	0.0	2.675	0.0	0.0	0.0
17	0.0	3.05	0.0	0.0	0.0
18	0.0	4.25	0.0	0.0	0.0
19	0.0	4.575	0.0	0.0	0.0
20	0.0	5.75	0.0	0.0	0.0
21	0.0	10.05	0.0	0.0	0.0
22	0.0	21.525	0.0	0.0	0.0
23	0.0	52.425	0.0	0.0	0.0
24	0.0	66.15	0.0	0.0	0.0
25	0.0	70.85	0.0	0.0	0.0
26	0.0	71.625	0.0	0.0	0.0
27	0.0	71.95	0.0	0.0	0.0
28	0.0	72.4	0.0	0.0	0.0
29	0.0	72.9	0.0	0.0	0.0
30	0.0	73.375	0.0	0.0	0.0
31	0.0	73.95	0.0	0.0	0.0
32	0.0	74.65	0.0	0.0	0.0
33	0.0	75.5	0.0	0.0	0.0
34	0.0	78.15	0.0	0.0	0.0
35	0.0	79.325	0.0	0.0	0.0
36	0.0	80.05	0.0	0.0	0.0
37	0.0	80.875	0.0	0.0	0.0
38	0.0	81.3	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCG	20	6.952051E-4	44.000004	8
GAGGTAG	20	6.952051E-4	44.000004	2
TCCTCGA	20	6.952051E-4	44.000004	9
AAGTAAT	40	5.315087E-9	44.000004	4
TCAAGTA	40	5.315087E-9	44.000004	2
AATCCAG	40	5.315087E-9	44.000004	8
TCGACTG	20	6.952051E-4	44.000004	12
ATCCAGG	40	5.315087E-9	44.000004	9
TTCAAGT	40	5.315087E-9	44.000004	1
AGGATAG	40	5.315087E-9	44.000004	13
CATCCTC	20	6.952051E-4	44.000004	7
ATAGGCT	40	5.315087E-9	44.000004	16
TCCAGGA	40	5.315087E-9	44.000004	10
GGATAGG	40	5.315087E-9	44.000004	14
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGTCG	20	6.952051E-4	44.000004	11
AGTAATC	40	5.315087E-9	44.000004	5
TAATCCA	40	5.315087E-9	44.000004	7
GGTAGTA	20	6.952051E-4	44.000004	4
GGAAGCT	20	6.952051E-4	44.000004	18
>>END_MODULE
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507624 spots for SRR5030925.sra
Written 507624 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
Read 507614 spots for SRR5030925.sra
Written 507614 spots for SRR5030925.sra
SRR ids: ['SRR5030925.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_mk30ps5q
SRR5030925.sra spots: 6091378
blocks: [[1, 507614], [507615, 1015228], [1015229, 1522842], [1522843, 2030456], [2030457, 2538070], [2538071, 3045684], [3045685, 3553298], [3553299, 4060912], [4060913, 4568526], [4568527, 5076140], [5076141, 5583754], [5583755, 6091378]]
SRR5030925 file size 1054698
SRR5030925 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030925 SRR5030925_1.fastq
Input file:	SRR5030925_1.fastq
trimmed:	SRR5030925-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:46:18 2020 >> started

Wed Mar  4 06:46:21 2020 >> done (3.493s)
6091378 reads processed; of these:
    257 ( 0.00%) short reads filtered out after trimming by size control
     13 ( 0.00%) empty reads filtered out after trimming by size control
6091108 (100.00%) reads available; of these:
 468197 ( 7.69%) trimmed reads available after processing
5622911 (92.31%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     91	  0.00%
 19	    129	  0.00%
 20	    302	  0.00%
 21	    333	  0.01%
 22	    601	  0.01%
 23	    613	  0.01%
 24	    725	  0.01%
 25	    845	  0.01%
 26	    758	  0.01%
 27	   1097	  0.02%
 28	   1319	  0.02%
 29	   2084	  0.03%
 30	   1486	  0.02%
 31	   1950	  0.03%
 32	   3079	  0.05%
 33	   5034	  0.08%
 34	  17294	  0.28%
 35	   7369	  0.12%
 36	   5517	  0.09%
 37	   5441	  0.09%
 38	   8045	  0.13%
 39	   7273	  0.12%
 40	  12899	  0.21%
 41	   9264	  0.15%
 42	  17962	  0.29%
 43	  11113	  0.18%
 44	  34250	  0.56%
 45	  34295	  0.56%
 46	  31602	  0.52%
 47	  37706	  0.62%
 48	  94692	  1.55%
 49	 113029	  1.86%
 50	5622911	 92.31%
6091108 reads passed initial QC


criterion=sequence-density
sequence-density=79.72
sequence-density-rank=1
fanout-score=19.41
fanout-score-rank=2
prefix-density=82.33
prefix-fanout=18.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC


criterion=fanout-score
sequence-density=2.46
sequence-density-rank=4
fanout-score=102.19
fanout-score-rank=1
prefix-density=82.12
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC -o SRR5030925 -
Input file:	STDIN
trimmed:	SRR5030925-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:46:43 2020 >> started

Wed Mar  4 06:46:51 2020 >> done (8.033s)
5938830 reads processed; of these:
 220742 ( 3.72%) short reads filtered out after trimming by size control
   8540 ( 0.14%) empty reads filtered out after trimming by size control
5709548 (96.14%) reads available; of these:
5072021 (88.83%) trimmed reads available after processing
 637527 (11.17%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  31088	  0.54%
 19	  62644	  1.10%
 20	 273488	  4.79%
 21	 613279	 10.74%
 22	1833493	 32.11%
 23	 801023	 14.03%
 24	 297048	  5.20%
 25	  55427	  0.97%
 26	  29220	  0.51%
 27	  29608	  0.52%
 28	  33095	  0.58%
 29	  27232	  0.48%
 30	  46098	  0.81%
 31	  61375	  1.07%
 32	  63722	  1.12%
 33	 164187	  2.88%
 34	  67465	  1.18%
 35	  48017	  0.84%
 36	  52397	  0.92%
 37	  32571	  0.57%
 38	  36793	  0.64%
 39	  36365	  0.64%
 40	  48271	  0.85%
 41	  62334	  1.09%
 42	  67930	  1.19%
 43	  53112	  0.93%
 44	  51773	  0.91%
 45	  56322	  0.99%
 46	  47675	  0.84%
 47	  41702	  0.73%
 48	   7502	  0.13%
 49	  24104	  0.42%
 50	 553188	  9.69%


criterion=sequence-density
sequence-density=7.16
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=1.35
sequence-density-rank=11
fanout-score=2.75
fanout-score-rank=1
prefix-density=0.03
prefix-fanout=2.8
sequence=TGAGGTAGTAGATTGTATAGTT
                                 Started job on |	Mar 04 06:47:12
                             Started mapping on |	Mar 04 06:47:13
                                    Finished on |	Mar 04 06:47:56
       Mapping speed, Million of reads per hour |	490.76

                          Number of input reads |	5861826
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3000991
                        Uniquely mapped reads % |	51.20%
                          Average mapped length |	27.63
                       Number of splices: Total |	2494
            Number of splices: Annotated (sjdb) |	521
                       Number of splices: GT/AG |	2093
                       Number of splices: GC/AG |	32
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	366
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.44
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.10
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2115219
             % of reads mapped to multiple loci |	36.08%
        Number of reads mapped to too many loci |	214009
             % of reads mapped to too many loci |	3.65%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.17%
                     % of reads unmapped: other |	0.90%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	745616	745616	745616
N_multimapping	2115219	2115219	2115219
N_noFeature	148223	181564	2319714
N_ambiguous	1599525	1027087	44069
UnstrandedReadsAssigned:1253243 PositiveStrandReadsAssigned:1792340 NegativeStrandReadsAssigned:637208
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030925 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030925-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,861,826 reads, 1,309,831 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,202 rounds

 109283 SRR5030925.ke.tsv
  52636 SRR5030925.se.tsv
 161919 total
==> SRR5030925.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030925.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030925 completed mapping pipeline successfully
