Starting /dee2/code/volunteer_pipeline.sh SRR5030981
    current disk space = 160584351744
    free memory = 73934212 
SRR5030981 SRAfilesize
fed084a42e5ed6be73ba021c830eb98d  SRR5030981.sra
SRR5030981.sra file validated
SRR5030981 is single end
SRR5030981 is conventional basespace
SRR5030981 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40225	33.0	31.0	34.0	31.0	34.0
2	32.75975	34.0	31.0	34.0	31.0	34.0
3	32.6885	34.0	31.0	34.0	31.0	34.0
4	36.25325	37.0	37.0	37.0	35.0	37.0
5	36.22025	37.0	37.0	37.0	35.0	37.0
6	35.85825	37.0	35.0	37.0	33.0	37.0
7	36.0365	37.0	35.0	37.0	35.0	37.0
8	36.16925	37.0	35.0	37.0	35.0	37.0
9	37.79125	39.0	38.0	39.0	35.0	39.0
10	37.96225	39.0	38.0	39.0	35.0	39.0
11	37.8315	39.0	38.0	39.0	35.0	39.0
12	37.81275	39.0	38.0	39.0	35.0	39.0
13	37.73425	39.0	38.0	39.0	35.0	39.0
14	39.04125	41.0	39.0	41.0	35.0	41.0
15	39.1515	41.0	39.0	41.0	36.0	41.0
16	39.23825	40.0	39.0	41.0	36.0	41.0
17	39.36625	41.0	39.0	41.0	36.0	41.0
18	39.27825	41.0	39.0	41.0	36.0	41.0
19	39.01375	40.0	39.0	41.0	35.0	41.0
20	39.003	40.0	39.0	41.0	35.0	41.0
21	38.94325	40.0	39.0	41.0	35.0	41.0
22	38.98425	40.0	39.0	41.0	35.0	41.0
23	38.8465	40.0	39.0	41.0	35.0	41.0
24	38.95025	40.0	39.0	41.0	35.0	41.0
25	38.967	40.0	39.0	41.0	35.0	41.0
26	38.74475	40.0	38.0	41.0	35.0	41.0
27	38.87575	40.0	39.0	41.0	35.0	41.0
28	38.63225	40.0	38.0	41.0	34.0	41.0
29	38.2775	40.0	38.0	41.0	33.0	41.0
30	38.51725	40.0	38.0	41.0	34.0	41.0
31	38.555	40.0	38.0	41.0	34.0	41.0
32	38.3625	40.0	38.0	41.0	34.0	41.0
33	38.212	40.0	38.0	41.0	34.0	41.0
34	38.1885	40.0	38.0	41.0	34.0	41.0
35	37.9735	40.0	38.0	41.0	33.0	41.0
36	37.528	40.0	37.0	41.0	32.0	41.0
37	37.508	40.0	37.0	41.0	31.0	41.0
38	37.479	40.0	37.0	41.0	31.0	41.0
39	37.81275	40.0	38.0	41.0	33.0	41.0
40	37.86825	40.0	38.0	41.0	33.0	41.0
41	37.967	40.0	38.0	41.0	33.0	41.0
42	38.16	40.0	38.0	41.0	33.0	41.0
43	38.2655	40.0	38.0	41.0	34.0	41.0
44	38.187	40.0	38.0	41.0	34.0	41.0
45	37.879	40.0	38.0	41.0	33.0	41.0
46	37.2965	40.0	37.0	41.0	31.0	41.0
47	36.8235	40.0	37.0	41.0	30.0	41.0
48	36.351	39.0	36.0	41.0	29.0	41.0
49	36.63725	39.0	36.0	41.0	30.0	41.0
50	36.05275	39.0	35.0	41.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	2.0
17	1.0
18	4.0
19	3.0
20	1.0
21	4.0
22	3.0
23	6.0
24	10.0
25	7.0
26	9.0
27	21.0
28	23.0
29	26.0
30	44.0
31	59.0
32	71.0
33	106.0
34	155.0
35	173.0
36	258.0
37	410.0
38	790.0
39	1811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.5075075075075075	21.07107107107107	59.20920920920921	12.212212212212211
2	24.525	26.5	21.275	27.700000000000003
3	18.85	19.375	20.325	41.449999999999996
4	28.175	36.25	12.775	22.8
5	23.275000000000002	38.05	28.225	10.45
6	36.95	15.35	36.875	10.825
7	11.85	24.525	25.224999999999998	38.4
8	24.6	40.300000000000004	13.0	22.1
9	18.925	37.875	30.425	12.775
10	19.325	20.424999999999997	27.525	32.725
11	29.425	10.674999999999999	15.35	44.55
12	15.75	15.35	25.45	43.45
13	22.675	22.125	36.775000000000006	18.425
14	38.95	24.099999999999998	24.325	12.625
15	39.15	21.275	28.025	11.55
16	12.0	25.074999999999996	21.875	41.05
17	25.35	21.4	40.625	12.625
18	29.299999999999997	18.15	29.5	23.05
19	40.475	13.175	29.875	16.475
20	51.449999999999996	17.075000000000003	21.275	10.2
21	20.3	26.424999999999997	25.5	27.775
22	32.425	11.675	49.6	6.3
23	25.025	12.3	50.575	12.1
24	52.025	12.525	30.8	4.65
25	55.95	21.625	15.15	7.2749999999999995
26	31.8	48.949999999999996	11.875	7.375
27	14.224999999999998	54.55	22.725	8.5
28	9.55	28.025	51.175000000000004	11.25
29	8.799999999999999	11.85	61.724999999999994	17.625
30	9.35	4.45	40.949999999999996	45.25
31	11.899999999999999	3.375	47.575	37.15
32	25.275	3.3000000000000003	24.775	46.650000000000006
33	56.55	5.2749999999999995	13.175	25.0
34	69.39999999999999	6.0249999999999995	11.275	13.3
35	70.475	5.525	16.475	7.5249999999999995
36	45.5	6.175	38.25	10.075000000000001
37	48.05	7.3999999999999995	22.75	21.8
38	25.674999999999997	13.15	11.575000000000001	49.6
39	13.525	22.825	6.675000000000001	56.974999999999994
40	11.725	50.525	8.05	29.7
41	22.1	55.65	6.7250000000000005	15.525
42	50.9	33.475	7.074999999999999	8.55
43	58.025000000000006	28.749999999999996	5.225	8.0
44	33.2	50.175000000000004	5.1	11.525
45	15.625	56.3	10.65	17.424999999999997
46	8.95	29.599999999999998	16.875	44.574999999999996
47	7.049999999999999	13.625000000000002	39.25	40.075
48	6.75	10.375	22.875	60.0
49	9.575	18.4	13.075000000000001	58.95
50	16.5	38.925	10.274999999999999	34.300000000000004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	0.5
26	0.0
27	0.5
28	1.0
29	1.0
30	1.0
31	2.0
32	3.0
33	5.5
34	8.0
35	8.0
36	8.0
37	11.0
38	14.0
39	20.5
40	27.0
41	28.0
42	29.0
43	69.5
44	110.0
45	172.0
46	234.0
47	433.5
48	633.0
49	635.5
50	638.0
51	816.0
52	994.0
53	681.0
54	368.0
55	395.0
56	422.0
57	346.0
58	270.0
59	171.0
60	72.0
61	57.5
62	43.0
63	52.0
64	61.0
65	40.5
66	20.0
67	12.0
68	4.0
69	6.0
70	8.0
71	18.0
72	28.0
73	15.0
74	2.0
75	1.0
76	0.0
77	0.0
78	0.0
79	0.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09211495946941	25.474999999999998
2	8.916728076639647	6.05
3	4.200442151805453	4.275
4	2.3581429624170966	3.2
5	1.3264554163596167	2.25
6	1.2527634487840826	2.55
7	0.8843036109064112	2.1
8	0.9579955784819455	2.6
9	0.7369196757553427	2.25
>10	3.6845983787767134	25.75
>50	0.2947678703021371	6.4750000000000005
>100	0.2947678703021371	17.025000000000002
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	266	6.65	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	197	4.925	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	113	2.825	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	105	2.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	61	1.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	57	1.425	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	45	1.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	39	0.975	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	38	0.95	RNA PCR Primer, Index 5 (97% over 35bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	25	0.625	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	20	0.5	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	13	0.325	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 48 (100% over 50bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTC	9	0.22499999999999998	RNA PCR Primer, Index 48 (100% over 44bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	8	0.2	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	7	0.17500000000000002	No Hit
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CTGCGTTCGCGCTCTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	6	0.15	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGAAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGATGATGAGAGCTTTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.275	0.0	0.0	0.0
2	0.0	0.275	0.0	0.0	0.0
3	0.0	0.325	0.0	0.0	0.0
4	0.0	0.325	0.0	0.0	0.0
5	0.0	0.4	0.0	0.0	0.0
6	0.0	0.425	0.0	0.0	0.0
7	0.0	0.8	0.0	0.0	0.0
8	0.0	0.825	0.0	0.0	0.0
9	0.0	0.95	0.0	0.0	0.0
10	0.0	1.075	0.0	0.0	0.0
11	0.0	1.25	0.0	0.0	0.0
12	0.0	1.7	0.0	0.0	0.0
13	0.0	1.85	0.0	0.0	0.0
14	0.0	2.025	0.0	0.0	0.0
15	0.0	2.3	0.0	0.0	0.0
16	0.0	3.75	0.0	0.0	0.0
17	0.0	4.55	0.0	0.0	0.0
18	0.0	5.8	0.0	0.0	0.0
19	0.0	6.425	0.0	0.0	0.0
20	0.0	7.625	0.0	0.0	0.0
21	0.0	12.525	0.0	0.0	0.0
22	0.0	24.6	0.0	0.0	0.0
23	0.0	57.825	0.0	0.0	0.0
24	0.0	75.825	0.0	0.0	0.0
25	0.0	82.75	0.0	0.0	0.0
26	0.0	83.95	0.0	0.0	0.0
27	0.0	84.525	0.0	0.0	0.0
28	0.0	85.075	0.0	0.0	0.0
29	0.0	85.45	0.0	0.0	0.0
30	0.0	85.85	0.0	0.0	0.0
31	0.0	86.8	0.0	0.0	0.0
32	0.0	87.8	0.0	0.0	0.0
33	0.0	88.8	0.0	0.0	0.0
34	0.0	90.325	0.0	0.0	0.0
35	0.0	91.225	0.0	0.0	0.0
36	0.0	91.725	0.0	0.0	0.0
37	0.0	92.325	0.0	0.0	0.0
38	0.0	92.675	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCCG	20	6.952051E-4	44.000004	8
AACGCTG	40	5.315087E-9	44.000004	8
TGTTGGA	20	6.952051E-4	44.000004	20
GAGGTAG	20	6.952051E-4	44.000004	2
ACGCTGT	40	5.315087E-9	44.000004	9
CTTTAAC	20	6.952051E-4	44.000004	12
TTCAACG	40	5.315087E-9	44.000004	5
CATCCCC	20	6.952051E-4	44.000004	7
ATAGGCT	40	5.315087E-9	44.000004	16
CATTCAA	45	2.728484E-10	44.000004	3
TCAACGC	40	5.315087E-9	44.000004	6
ACATCCC	20	6.952051E-4	44.000004	6
ACATTCA	45	2.728484E-10	44.000004	2
GGATAGG	45	2.728484E-10	44.000004	14
TAACCTG	20	6.952051E-4	44.000004	15
CCCCGAC	20	6.952051E-4	44.000004	10
GCTGTCG	40	5.315087E-9	44.000004	11
CCCGACT	20	6.952051E-4	44.000004	11
CCTTTAA	20	6.952051E-4	44.000004	11
TGAGGTA	20	6.952051E-4	44.000004	1
>>END_MODULE
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188063 spots for SRR5030981.sra
Written 188063 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
Read 188059 spots for SRR5030981.sra
Written 188059 spots for SRR5030981.sra
SRR ids: ['SRR5030981.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_eh14da5t
SRR5030981.sra spots: 2256712
blocks: [[1, 188059], [188060, 376118], [376119, 564177], [564178, 752236], [752237, 940295], [940296, 1128354], [1128355, 1316413], [1316414, 1504472], [1504473, 1692531], [1692532, 1880590], [1880591, 2068649], [2068650, 2256712]]
SRR5030981 file size 390062
SRR5030981 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030981 SRR5030981_1.fastq
Input file:	SRR5030981_1.fastq
trimmed:	SRR5030981-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:59:00 2020 >> started

Wed Mar  4 06:59:01 2020 >> done (1.539s)
2256712 reads processed; of these:
    129 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2256577 (99.99%) reads available; of these:
 147103 ( 6.52%) trimmed reads available after processing
2109474 (93.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     43	  0.00%
 19	     59	  0.00%
 20	     93	  0.00%
 21	    134	  0.01%
 22	    258	  0.01%
 23	    267	  0.01%
 24	    254	  0.01%
 25	    358	  0.02%
 26	    309	  0.01%
 27	    438	  0.02%
 28	    548	  0.02%
 29	    857	  0.04%
 30	    548	  0.02%
 31	    599	  0.03%
 32	    614	  0.03%
 33	    878	  0.04%
 34	   1133	  0.05%
 35	   1975	  0.09%
 36	   1867	  0.08%
 37	   1785	  0.08%
 38	   1827	  0.08%
 39	   1936	  0.09%
 40	   2412	  0.11%
 41	   2656	  0.12%
 42	   3877	  0.17%
 43	   5303	  0.24%
 44	   7743	  0.34%
 45	  10302	  0.46%
 46	  13883	  0.62%
 47	  16504	  0.73%
 48	  28397	  1.26%
 49	  39246	  1.74%
 50	2109474	 93.48%
2256577 reads passed initial QC


criterion=sequence-density
sequence-density=92.38
sequence-density-rank=1
fanout-score=18.31
fanout-score-rank=1
prefix-density=93.84
prefix-fanout=18.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC


criterion=fanout-score
sequence-density=92.38
sequence-density-rank=1
fanout-score=18.31
fanout-score-rank=1
prefix-density=93.84
prefix-fanout=18.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC -o SRR5030981 -
Input file:	STDIN
trimmed:	SRR5030981-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:59:14 2020 >> started

Wed Mar  4 06:59:17 2020 >> done (3.314s)
2208049 reads processed; of these:
 134543 ( 6.09%) short reads filtered out after trimming by size control
   6877 ( 0.31%) empty reads filtered out after trimming by size control
2066629 (93.60%) reads available; of these:
2026580 (98.06%) trimmed reads available after processing
  40049 ( 1.94%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13124	  0.64%
 19	  27990	  1.35%
 20	 111868	  5.41%
 21	 263186	 12.74%
 22	 770657	 37.29%
 23	 392347	 18.98%
 24	 142636	  6.90%
 25	  23135	  1.12%
 26	  11865	  0.57%
 27	  11336	  0.55%
 28	  12185	  0.59%
 29	   9285	  0.45%
 30	  17714	  0.86%
 31	  22758	  1.10%
 32	  22940	  1.11%
 33	  37111	  1.80%
 34	  18775	  0.91%
 35	  11737	  0.57%
 36	  13032	  0.63%
 37	   7760	  0.38%
 38	   7892	  0.38%
 39	   7202	  0.35%
 40	   9461	  0.46%
 41	  13454	  0.65%
 42	  14141	  0.68%
 43	  10391	  0.50%
 44	   8547	  0.41%
 45	   9458	  0.46%
 46	   7122	  0.34%
 47	   5182	  0.25%
 48	    485	  0.02%
 49	    792	  0.04%
 50	  31061	  1.50%


criterion=sequence-density
sequence-density=10.25
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.45
sequence-density-rank=33
fanout-score=16.70
fanout-score-rank=1
prefix-density=7.52
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:59:37
                             Started mapping on |	Mar 04 06:59:38
                                    Finished on |	Mar 04 07:00:03
       Mapping speed, Million of reads per hour |	304.58

                          Number of input reads |	2115157
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1042991
                        Uniquely mapped reads % |	49.31%
                          Average mapped length |	23.37
                       Number of splices: Total |	938
            Number of splices: Annotated (sjdb) |	117
                       Number of splices: GT/AG |	865
                       Number of splices: GC/AG |	10
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	63
                      Mismatch rate per base, % |	0.12%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.86
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	893709
             % of reads mapped to multiple loci |	42.25%
        Number of reads mapped to too many loci |	38928
             % of reads mapped to too many loci |	1.84%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.89%
                     % of reads unmapped: other |	0.70%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	178457	178457	178457
N_multimapping	893709	893709	893709
N_noFeature	42375	50779	774190
N_ambiguous	574594	339087	6469
UnstrandedReadsAssigned:426022 PositiveStrandReadsAssigned:653125 NegativeStrandReadsAssigned:262332
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030981 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030981-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,115,157 reads, 432,454 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,096 rounds

 109283 SRR5030981.ke.tsv
  52636 SRR5030981.se.tsv
 161919 total
==> SRR5030981.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030981.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030981 completed mapping pipeline successfully
