Starting /dee2/code/volunteer_pipeline.sh SRR5031040
    current disk space = 160516587520
    free memory = 73932448 
SRR5031040 SRAfilesize
77f4abaf31e662c1560688f0153af054  SRR5031040.sra
SRR5031040.sra file validated
SRR5031040 is single end
SRR5031040 is conventional basespace
SRR5031040 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43225	33.0	31.0	34.0	31.0	34.0
2	32.6105	34.0	31.0	34.0	31.0	34.0
3	32.671	34.0	31.0	34.0	31.0	34.0
4	36.22725	37.0	37.0	37.0	35.0	37.0
5	36.13525	37.0	35.0	37.0	35.0	37.0
6	35.90025	37.0	35.0	37.0	35.0	37.0
7	36.0165	37.0	35.0	37.0	35.0	37.0
8	35.909	37.0	35.0	37.0	35.0	37.0
9	37.6085	39.0	37.0	39.0	35.0	39.0
10	37.82175	39.0	38.0	39.0	35.0	39.0
11	37.707	39.0	38.0	39.0	35.0	39.0
12	37.651	39.0	37.0	39.0	35.0	39.0
13	37.70225	39.0	37.0	39.0	35.0	39.0
14	39.06025	41.0	38.0	41.0	35.0	41.0
15	39.0125	40.0	38.0	41.0	36.0	41.0
16	39.04925	40.0	38.0	41.0	36.0	41.0
17	38.946	40.0	38.0	41.0	35.0	41.0
18	39.1375	41.0	39.0	41.0	36.0	41.0
19	38.79575	40.0	38.0	41.0	35.0	41.0
20	38.9025	40.0	39.0	41.0	35.0	41.0
21	38.881	40.0	38.0	41.0	35.0	41.0
22	39.01925	40.0	39.0	41.0	36.0	41.0
23	38.8785	40.0	38.0	41.0	35.0	41.0
24	38.942	40.0	38.0	41.0	35.0	41.0
25	39.16525	41.0	39.0	41.0	36.0	41.0
26	38.93425	40.0	38.0	41.0	35.0	41.0
27	38.82075	40.0	39.0	41.0	35.0	41.0
28	38.85375	40.0	38.0	41.0	35.0	41.0
29	38.42275	40.0	38.0	41.0	34.0	41.0
30	38.343	40.0	38.0	41.0	34.0	41.0
31	38.5745	40.0	38.0	41.0	34.0	41.0
32	38.6185	40.0	38.0	41.0	34.0	41.0
33	38.7445	40.0	38.0	41.0	35.0	41.0
34	38.76075	40.0	38.0	41.0	35.0	41.0
35	38.53475	40.0	38.0	41.0	35.0	41.0
36	38.235	40.0	38.0	41.0	34.0	41.0
37	37.6775	40.0	37.0	41.0	32.0	41.0
38	36.912	40.0	36.0	41.0	30.0	41.0
39	37.29175	40.0	37.0	41.0	31.0	41.0
40	37.70125	40.0	37.0	41.0	33.0	41.0
41	37.71575	40.0	37.0	41.0	32.0	41.0
42	37.9515	40.0	38.0	41.0	33.0	41.0
43	38.24525	40.0	38.0	41.0	34.0	41.0
44	37.95225	40.0	38.0	41.0	33.0	41.0
45	37.81225	40.0	37.0	41.0	33.0	41.0
46	37.85175	40.0	37.0	41.0	33.0	41.0
47	37.4345	40.0	37.0	41.0	32.0	41.0
48	37.10025	40.0	36.0	41.0	31.0	41.0
49	37.29575	40.0	37.0	41.0	32.0	41.0
50	36.72125	40.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	2.0
17	2.0
18	3.0
19	2.0
20	0.0
21	7.0
22	4.0
23	4.0
24	5.0
25	14.0
26	13.0
27	17.0
28	18.0
29	23.0
30	42.0
31	62.0
32	99.0
33	101.0
34	162.0
35	179.0
36	236.0
37	395.0
38	682.0
39	1926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.75	24.575	53.37499999999999	14.299999999999999
2	22.726133801052367	26.659984966173887	18.917564520170384	31.696316712603355
3	23.625	20.9	22.525000000000002	32.95
4	31.674999999999997	28.775000000000002	13.925	25.624999999999996
5	21.575	41.775	25.575	11.075
6	34.975	15.45	33.825	15.75
7	15.174999999999999	21.9	23.200000000000003	39.725
8	26.200000000000003	29.675	15.5	28.625
9	21.425	33.375	29.675	15.525
10	20.849999999999998	22.925	28.375	27.85
11	30.0	9.049999999999999	18.2	42.75
12	14.575	16.900000000000002	31.974999999999998	36.55
13	31.225	19.2	31.1	18.475
14	32.824999999999996	32.6	18.7	15.875
15	34.55	26.150000000000002	26.125	13.175
16	17.025000000000002	21.575	22.425	38.975
17	23.974999999999998	21.725	40.699999999999996	13.600000000000001
18	25.174999999999997	17.45	30.175	27.200000000000003
19	37.775	13.700000000000001	30.275000000000002	18.25
20	43.375	15.65	27.900000000000002	13.075000000000001
21	21.725	20.474999999999998	29.625	28.175
22	29.025000000000002	12.5	47.675	10.8
23	28.575	12.725	43.125	15.575
24	51.925	14.725	26.6	6.75
25	50.1	26.55	14.75	8.6
26	28.4	47.075	14.625	9.9
27	16.1	47.475	25.424999999999997	11.0
28	10.125	25.025	49.675000000000004	15.174999999999999
29	10.65	13.475000000000001	56.3	19.575
30	8.95	6.5	38.6	45.95
31	14.2	4.625	46.725	34.449999999999996
32	29.325000000000003	5.7250000000000005	21.224999999999998	43.725
33	55.300000000000004	8.25	13.4	23.05
34	62.575	7.000000000000001	16.325	14.099999999999998
35	65.10000000000001	6.0	19.650000000000002	9.25
36	44.2	6.15	35.699999999999996	13.950000000000001
37	45.050000000000004	8.95	20.375	25.624999999999996
38	23.925	15.35	12.049999999999999	48.675000000000004
39	16.3	25.124999999999996	9.225	49.35
40	16.2	48.125	9.375	26.3
41	26.5	48.375	8.075000000000001	17.05
42	49.3	30.9	10.925	8.875
43	51.775000000000006	31.825	7.3	9.1
44	30.975	49.05	7.199999999999999	12.775
45	17.95	49.95	12.6	19.5
46	12.275	27.425	18.65	41.65
47	7.5249999999999995	15.725	37.925	38.824999999999996
48	9.3	13.55	20.7	56.45
49	12.1	20.075000000000003	14.424999999999999	53.400000000000006
50	18.825	35.375	15.225	30.575000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	1.0
25	0.5
26	0.0
27	1.5
28	3.0
29	2.5
30	2.0
31	8.5
32	15.0
33	13.0
34	11.0
35	14.5
36	18.0
37	20.5
38	23.0
39	31.5
40	40.0
41	42.5
42	45.0
43	68.0
44	91.0
45	142.5
46	194.0
47	333.0
48	472.0
49	589.0
50	706.0
51	813.0
52	920.0
53	677.0
54	434.0
55	457.5
56	481.0
57	345.5
58	210.0
59	132.5
60	55.0
61	50.0
62	45.0
63	84.0
64	123.0
65	74.5
66	26.0
67	22.0
68	18.0
69	16.0
70	14.0
71	30.0
72	46.0
73	24.0
74	2.0
75	1.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.22499999999999998
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.175000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22252937111264	26.85
2	9.606081548030408	6.950000000000001
3	4.561161022805805	4.95
4	2.7643400138217	4.0
5	1.9350380096751902	3.5000000000000004
6	0.9675190048375951	2.1
7	0.8984105044920525	2.275
8	0.48375950241879756	1.4000000000000001
9	0.7601935038009675	2.475
>10	3.1789910158949555	23.575
>50	0.27643400138217	7.3999999999999995
>100	0.3455425017277125	14.524999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	133	3.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	132	3.3000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	107	2.675	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	105	2.625	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	104	2.6	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	93	2.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	77	1.925	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	73	1.825	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	42	1.05	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	39	0.975	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	32	0.8	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	24	0.6	RNA PCR Primer, Index 1 (97% over 35bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	23	0.575	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC	15	0.375	RNA PCR Primer, Index 12 (100% over 44bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	15	0.375	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	11	0.27499999999999997	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	9	0.22499999999999998	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	7	0.17500000000000002	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAATACTGTCTGGTAATGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC	6	0.15	RNA PCR Primer, Index 12 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.15	0.0	0.0	0.0
2	0.0	0.15	0.0	0.0	0.0
3	0.0	0.25	0.0	0.0	0.0
4	0.0	0.3	0.0	0.0	0.0
5	0.0	0.325	0.0	0.0	0.0
6	0.0	0.4	0.0	0.0	0.0
7	0.0	0.925	0.0	0.0	0.0
8	0.0	1.125	0.0	0.0	0.0
9	0.0	1.15	0.0	0.0	0.0
10	0.0	1.3	0.0	0.0	0.0
11	0.0	1.425	0.0	0.0	0.0
12	0.0	1.6	0.0	0.0	0.0
13	0.0	1.925	0.0	0.0	0.0
14	0.0	2.075	0.0	0.0	0.0
15	0.0	2.475	0.0	0.0	0.0
16	0.0	3.525	0.0	0.0	0.0
17	0.0	4.1	0.0	0.0	0.0
18	0.0	5.025	0.0	0.0	0.0
19	0.0	5.425	0.0	0.0	0.0
20	0.0	6.25	0.0	0.0	0.0
21	0.0	12.325	0.0	0.0	0.0
22	0.0	25.475	0.0	0.0	0.0
23	0.0	54.85	0.0	0.0	0.0
24	0.0	68.775	0.0	0.0	0.0
25	0.0	75.6	0.0	0.0	0.0
26	0.0	77.575	0.0	0.0	0.0
27	0.0	78.275	0.0	0.0	0.0
28	0.0	79.075	0.0	0.0	0.0
29	0.0	79.9	0.0	0.0	0.0
30	0.0	80.425	0.0	0.0	0.0
31	0.0	81.375	0.0	0.0	0.0
32	0.0	82.15	0.0	0.0	0.0
33	0.0	82.975	0.0	0.0	0.0
34	0.0	85.7	0.0	0.0	0.0
35	0.0	86.55	0.0	0.0	0.0
36	0.0	87.1	0.0	0.0	0.0
37	0.0	87.85	0.0	0.0	0.0
38	0.0	88.3	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTTG	20	6.952051E-4	44.000004	18
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
GGTGAGT	20	6.952051E-4	44.000004	17
GTTGAAG	20	6.952051E-4	44.000004	10
AACCGGT	20	6.952051E-4	44.000004	9
CAGTTGA	20	6.952051E-4	44.000004	8
CTGAGCT	40	5.315087E-9	44.000004	11
ACTTGTG	20	6.952051E-4	44.000004	15
CCAGTTG	20	6.952051E-4	44.000004	7
AACTGTT	20	6.952051E-4	44.000004	17
GGTCTCT	20	6.952051E-4	44.000004	13
GAGCTTG	40	5.315087E-9	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
TCGGATC	40	5.315087E-9	44.000004	1
CGGATCC	40	5.315087E-9	44.000004	2
GAAGAAC	20	6.952051E-4	44.000004	13
AGAACTG	20	6.952051E-4	44.000004	15
GCCAGTT	20	6.952051E-4	44.000004	6
>>END_MODULE
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369704 spots for SRR5031040.sra
Written 369704 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
Read 369699 spots for SRR5031040.sra
Written 369699 spots for SRR5031040.sra
SRR ids: ['SRR5031040.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x129oj6d
SRR5031040.sra spots: 4436393
blocks: [[1, 369699], [369700, 739398], [739399, 1109097], [1109098, 1478796], [1478797, 1848495], [1848496, 2218194], [2218195, 2587893], [2587894, 2957592], [2957593, 3327291], [3327292, 3696990], [3696991, 4066689], [4066690, 4436393]]
SRR5031040 file size 767875
SRR5031040 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031040 SRR5031040_1.fastq
Input file:	SRR5031040_1.fastq
trimmed:	SRR5031040-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:11:40 2020 >> started

Wed Mar  4 07:11:42 2020 >> done (2.326s)
4436393 reads processed; of these:
    300 ( 0.01%) short reads filtered out after trimming by size control
     10 ( 0.00%) empty reads filtered out after trimming by size control
4436083 (99.99%) reads available; of these:
 209850 ( 4.73%) trimmed reads available after processing
4226233 (95.27%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    104	  0.00%
 19	    113	  0.00%
 20	    258	  0.01%
 21	    327	  0.01%
 22	    550	  0.01%
 23	    587	  0.01%
 24	    646	  0.01%
 25	    901	  0.02%
 26	    689	  0.02%
 27	    922	  0.02%
 28	   1054	  0.02%
 29	   1823	  0.04%
 30	   1163	  0.03%
 31	   1301	  0.03%
 32	   1369	  0.03%
 33	   1922	  0.04%
 34	   2271	  0.05%
 35	   4297	  0.10%
 36	   3779	  0.09%
 37	   4419	  0.10%
 38	   3967	  0.09%
 39	   4218	  0.10%
 40	   4622	  0.10%
 41	   4728	  0.11%
 42	   5991	  0.14%
 43	   8283	  0.19%
 44	  11446	  0.26%
 45	  13767	  0.31%
 46	  19465	  0.44%
 47	  25690	  0.58%
 48	  40352	  0.91%
 49	  38826	  0.88%
 50	4226233	 95.27%
4436083 reads passed initial QC


criterion=sequence-density
sequence-density=88.77
sequence-density-rank=1
fanout-score=20.26
fanout-score-rank=1
prefix-density=90.44
prefix-fanout=19.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC


criterion=fanout-score
sequence-density=88.77
sequence-density-rank=1
fanout-score=20.26
fanout-score-rank=1
prefix-density=90.44
prefix-fanout=19.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC -o SRR5031040 -
Input file:	STDIN
trimmed:	SRR5031040-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:12:02 2020 >> started

Wed Mar  4 07:12:09 2020 >> done (6.810s)
4336396 reads processed; of these:
 217447 ( 5.01%) short reads filtered out after trimming by size control
  16444 ( 0.38%) empty reads filtered out after trimming by size control
4102505 (94.61%) reads available; of these:
3954974 (96.40%) trimmed reads available after processing
 147531 ( 3.60%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  21475	  0.52%
 19	  42502	  1.04%
 20	 245385	  5.98%
 21	 558328	 13.61%
 22	1374844	 33.51%
 23	 603976	 14.72%
 24	 291383	  7.10%
 25	  78355	  1.91%
 26	  37869	  0.92%
 27	  28214	  0.69%
 28	  30571	  0.75%
 29	  22668	  0.55%
 30	  32146	  0.78%
 31	  36723	  0.90%
 32	  42945	  1.05%
 33	 124669	  3.04%
 34	  38688	  0.94%
 35	  27926	  0.68%
 36	  31737	  0.77%
 37	  18983	  0.46%
 38	  20455	  0.50%
 39	  20007	  0.49%
 40	  31442	  0.77%
 41	  41405	  1.01%
 42	  46968	  1.14%
 43	  32224	  0.79%
 44	  24067	  0.59%
 45	  32562	  0.79%
 46	  22686	  0.55%
 47	  16767	  0.41%
 48	   1581	  0.04%
 49	   2049	  0.05%
 50	 120905	  2.95%


criterion=sequence-density
sequence-density=6.19
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=39
fanout-score=91.28
fanout-score-rank=1
prefix-density=1.72
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 07:12:46
                             Started mapping on |	Mar 04 07:12:47
                                    Finished on |	Mar 04 07:13:15
       Mapping speed, Million of reads per hour |	540.28

                          Number of input reads |	4202192
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2299510
                        Uniquely mapped reads % |	54.72%
                          Average mapped length |	24.08
                       Number of splices: Total |	2617
            Number of splices: Annotated (sjdb) |	595
                       Number of splices: GT/AG |	2456
                       Number of splices: GC/AG |	30
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	130
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.76
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.10
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1426757
             % of reads mapped to multiple loci |	33.95%
        Number of reads mapped to too many loci |	65974
             % of reads mapped to too many loci |	1.57%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.22%
                     % of reads unmapped: other |	0.54%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	475925	475925	475925
N_multimapping	1426757	1426757	1426757
N_noFeature	92990	116918	1582357
N_ambiguous	1283772	663903	18637
UnstrandedReadsAssigned:922748 PositiveStrandReadsAssigned:1518689 NegativeStrandReadsAssigned:698516
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031040 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031040-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,202,192 reads, 1,098,590 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,163 rounds

 109283 SRR5031040.ke.tsv
  52636 SRR5031040.se.tsv
 161919 total
==> SRR5031040.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031040.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031040 completed mapping pipeline successfully
