Starting /dee2/code/volunteer_pipeline.sh SRR5031064
    current disk space = 160514064384
    free memory = 56707260 
SRR5031064 SRAfilesize
360d53b428c4cffce9ac196700f660ac  SRR5031064.sra
SRR5031064.sra file validated
SRR5031064 is single end
SRR5031064 is conventional basespace
SRR5031064 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3355	33.0	31.0	34.0	31.0	34.0
2	32.5545	34.0	31.0	34.0	31.0	34.0
3	32.5515	34.0	31.0	34.0	31.0	34.0
4	36.14475	37.0	35.0	37.0	35.0	37.0
5	36.163	37.0	35.0	37.0	35.0	37.0
6	35.80425	37.0	35.0	37.0	33.0	37.0
7	35.91275	37.0	35.0	37.0	35.0	37.0
8	35.89425	37.0	35.0	37.0	35.0	37.0
9	37.681	39.0	37.0	39.0	35.0	39.0
10	37.812	39.0	38.0	39.0	35.0	39.0
11	37.61825	39.0	37.0	39.0	35.0	39.0
12	37.66975	39.0	37.0	39.0	35.0	39.0
13	37.582	39.0	37.0	39.0	35.0	39.0
14	39.0045	40.0	38.0	41.0	35.0	41.0
15	39.0045	40.0	38.0	41.0	36.0	41.0
16	39.032	40.0	38.0	41.0	36.0	41.0
17	38.80025	40.0	38.0	41.0	34.0	41.0
18	38.9895	40.0	38.0	41.0	35.0	41.0
19	38.53825	40.0	38.0	41.0	34.0	41.0
20	38.733	40.0	38.0	41.0	35.0	41.0
21	38.749	40.0	38.0	41.0	34.0	41.0
22	38.843	40.0	38.0	41.0	35.0	41.0
23	38.564	40.0	38.0	41.0	34.0	41.0
24	38.485	40.0	38.0	41.0	34.0	41.0
25	38.568	40.0	38.0	41.0	34.0	41.0
26	38.16275	40.0	38.0	41.0	33.0	41.0
27	38.41475	40.0	38.0	41.0	34.0	41.0
28	38.31225	40.0	38.0	41.0	34.0	41.0
29	38.04025	40.0	38.0	41.0	33.0	41.0
30	37.90125	40.0	38.0	41.0	32.0	41.0
31	38.217	40.0	38.0	41.0	33.0	41.0
32	38.29075	40.0	38.0	41.0	33.0	41.0
33	38.42175	40.0	38.0	41.0	34.0	41.0
34	38.7125	40.0	38.0	41.0	35.0	41.0
35	38.43475	40.0	38.0	41.0	34.0	41.0
36	38.08175	40.0	38.0	41.0	33.0	41.0
37	37.70625	40.0	37.0	41.0	32.0	41.0
38	36.71025	39.0	36.0	41.0	30.0	41.0
39	37.23525	39.0	37.0	41.0	30.0	41.0
40	37.59625	40.0	37.0	41.0	32.0	41.0
41	37.83	40.0	38.0	41.0	33.0	41.0
42	38.04025	40.0	38.0	41.0	33.0	41.0
43	38.24525	40.0	38.0	41.0	34.0	41.0
44	37.9495	40.0	38.0	41.0	33.0	41.0
45	37.87225	40.0	37.0	41.0	33.0	41.0
46	37.79775	40.0	37.0	41.0	33.0	41.0
47	37.4105	40.0	37.0	41.0	31.0	41.0
48	36.877	40.0	36.0	41.0	31.0	41.0
49	37.27775	40.0	37.0	41.0	31.0	41.0
50	36.8895	40.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	2.0
17	1.0
18	2.0
19	2.0
20	3.0
21	4.0
22	4.0
23	6.0
24	6.0
25	11.0
26	16.0
27	27.0
28	27.0
29	34.0
30	47.0
31	76.0
32	83.0
33	105.0
34	161.0
35	188.0
36	261.0
37	428.0
38	669.0
39	1835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.225	23.400000000000002	56.10000000000001	14.274999999999999
2	23.59578736208626	28.986960882647946	20.86258776328987	26.55466399197593
3	16.875	18.15	18.45	46.525
4	25.874999999999996	37.3	11.65	25.174999999999997
5	22.400000000000002	38.324999999999996	28.65	10.625
6	38.05	13.825000000000001	36.025	12.1
7	11.875	25.525	25.15	37.45
8	23.974999999999998	41.725	13.725000000000001	20.575
9	17.349999999999998	41.05	28.65	12.950000000000001
10	17.599999999999998	21.375	26.150000000000002	34.875
11	31.1	11.799999999999999	13.925	43.175000000000004
12	14.875	15.625	23.599999999999998	45.9
13	20.25	22.85	38.775	18.125
14	38.975	22.525000000000002	25.174999999999997	13.325000000000001
15	38.1	23.200000000000003	28.025	10.674999999999999
16	14.274999999999999	28.15	18.85	38.725
17	25.025	23.5	38.675	12.8
18	27.025	19.825	26.3	26.85
19	39.125	13.4	29.075	18.4
20	51.55	15.35	23.925	9.175
21	22.725	25.174999999999997	22.7	29.4
22	32.550000000000004	11.4	49.275000000000006	6.775
23	23.7	11.325000000000001	51.65	13.325000000000001
24	55.400000000000006	10.674999999999999	27.775	6.15
25	58.650000000000006	22.125	12.625	6.6000000000000005
26	29.125	51.324999999999996	10.625	8.924999999999999
27	12.7	56.825	20.200000000000003	10.274999999999999
28	7.475	26.875	52.800000000000004	12.85
29	9.25	10.674999999999999	63.05	17.025000000000002
30	7.175	4.45	39.275	49.1
31	11.175	3.225	51.025	34.575
32	25.124999999999996	4.125	23.275000000000002	47.475
33	57.85	6.7	11.675	23.775
34	70.5	5.7250000000000005	11.425	12.35
35	70.975	5.55	15.825	7.6499999999999995
36	43.125	5.4	41.6	9.875
37	48.5	8.25	22.7	20.549999999999997
38	24.375	12.85	10.65	52.125
39	12.2	20.8	8.200000000000001	58.8
40	11.75	51.949999999999996	8.275	28.025
41	20.45	58.45	6.4	14.7
42	52.725	32.175	8.200000000000001	6.9
43	59.9	25.525	6.425	8.15
44	32.525	51.9	5.4	10.174999999999999
45	15.825	58.475	9.5	16.2
46	10.0	28.050000000000004	15.775	46.175
47	6.7250000000000005	12.225	43.675000000000004	37.375
48	8.475000000000001	9.700000000000001	22.05	59.775
49	9.3	16.925	11.275	62.5
50	15.675	42.125	9.2	33.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	1.0
30	2.0
31	6.5
32	11.0
33	14.0
34	17.0
35	15.0
36	13.0
37	11.0
38	9.0
39	18.0
40	27.0
41	36.0
42	45.0
43	83.0
44	121.0
45	163.0
46	205.0
47	420.0
48	635.0
49	618.0
50	601.0
51	825.0
52	1049.0
53	691.5
54	334.0
55	343.0
56	352.0
57	316.5
58	281.0
59	166.5
60	52.0
61	44.0
62	36.0
63	82.0
64	128.0
65	74.5
66	21.0
67	15.5
68	10.0
69	13.5
70	17.0
71	19.5
72	22.0
73	13.0
74	4.0
75	2.5
76	1.0
77	2.0
78	3.0
79	2.0
80	1.0
81	1.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.3
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.550000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66467958271237	25.05
2	9.910581222056631	6.65
3	4.023845007451564	4.05
4	2.086438152011923	2.8000000000000003
5	1.8628912071535022	3.125
6	0.5961251862891207	1.2
7	1.4157973174366618	3.325
8	0.5961251862891207	1.6
9	0.29806259314456035	0.8999999999999999
>10	3.9493293591654246	24.775
>50	0.29806259314456035	7.074999999999999
>100	0.29806259314456035	19.45
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	291	7.2749999999999995	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	178	4.45	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	157	3.925	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	152	3.8	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	86	2.15	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	46	1.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	38	0.95	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	31	0.775	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC	15	0.375	RNA PCR Primer, Index 5 (97% over 35bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	12	0.3	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	12	0.3	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGC	10	0.25	RNA PCR Primer, Index 36 (100% over 50bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTC	8	0.2	RNA PCR Primer, Index 36 (100% over 44bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
AGAGGTAGTAGGTTGCATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.275	0.0	0.0	0.0
2	0.0	0.275	0.0	0.0	0.0
3	0.0	0.35	0.0	0.0	0.0
4	0.0	0.375	0.0	0.0	0.0
5	0.0	0.425	0.0	0.0	0.0
6	0.0	0.475	0.0	0.0	0.0
7	0.0	0.85	0.0	0.0	0.0
8	0.0	0.9	0.0	0.0	0.0
9	0.0	0.975	0.0	0.0	0.0
10	0.0	1.075	0.0	0.0	0.0
11	0.0	1.275	0.0	0.0	0.0
12	0.0	1.475	0.0	0.0	0.0
13	0.0	1.675	0.0	0.0	0.0
14	0.0	1.925	0.0	0.0	0.0
15	0.0	2.1	0.0	0.0	0.0
16	0.0	2.6	0.0	0.0	0.0
17	0.0	3.025	0.0	0.0	0.0
18	0.0	3.65	0.0	0.0	0.0
19	0.0	4.2	0.0	0.0	0.0
20	0.0	4.925	0.0	0.0	0.0
21	0.0	9.05	0.0	0.0	0.0
22	0.0	20.125	0.0	0.0	0.0
23	0.0	56.1	0.0	0.0	0.0
24	0.0	73.275	0.0	0.0	0.0
25	0.0	79.2	0.0	0.0	0.0
26	0.0	80.25	0.0	0.0	0.0
27	0.0	80.5	0.0	0.0	0.0
28	0.0	80.825	0.0	0.0	0.0
29	0.0	81.125	0.0	0.0	0.0
30	0.0	81.625	0.0	0.0	0.0
31	0.0	82.5	0.0	0.0	0.0
32	0.0	83.375	0.0	0.0	0.0
33	0.0	84.175	0.0	0.0	0.0
34	0.0	87.175	0.0	0.0	0.0
35	0.0	88.1	0.0	0.0	0.0
36	0.0	88.65	0.0	0.0	0.0
37	0.0	89.2	0.0	0.0	0.0
38	0.0	89.7	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	20	6.952051E-4	44.000004	1
TAAACAT	45	2.728484E-10	44.000004	3
AAACATC	45	2.728484E-10	44.000004	4
GAGTGGA	20	6.952051E-4	44.000004	20
GCGACCT	20	6.952051E-4	44.000004	2
GCGACCC	20	6.952051E-4	44.000004	22
CCGCTGA	20	6.952051E-4	44.000004	27
ATAGGCT	45	2.728484E-10	44.000004	16
AGTGGAA	20	6.952051E-4	44.000004	21
GGCGACC	20	6.952051E-4	44.000004	21
ACCCGCT	20	6.952051E-4	44.000004	25
GTGAGTG	20	6.952051E-4	44.000004	18
ACATCCT	45	2.728484E-10	44.000004	6
CCCGCTG	20	6.952051E-4	44.000004	26
CGTGGCG	20	6.952051E-4	44.000004	18
ATCAGAC	20	6.952051E-4	44.000004	12
AGACGTG	20	6.952051E-4	44.000004	15
GACGTGG	20	6.952051E-4	44.000004	16
TCAGATC	20	6.952051E-4	44.000004	8
AGGCTTG	40	5.315087E-9	44.000004	18
>>END_MODULE
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
Read 503209 spots for SRR5031064.sra
Written 503209 spots for SRR5031064.sra
Read 503204 spots for SRR5031064.sra
Written 503204 spots for SRR5031064.sra
SRR ids: ['SRR5031064.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ptuxmwzy
SRR5031064.sra spots: 6038453
blocks: [[1, 503204], [503205, 1006408], [1006409, 1509612], [1509613, 2012816], [2012817, 2516020], [2516021, 3019224], [3019225, 3522428], [3522429, 4025632], [4025633, 4528836], [4528837, 5032040], [5032041, 5535244], [5535245, 6038453]]
SRR5031064 file size 1045549
SRR5031064 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031064 SRR5031064_1.fastq
Input file:	SRR5031064_1.fastq
trimmed:	SRR5031064-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:13 2020 >> started

Wed Mar  4 07:17:17 2020 >> done (3.518s)
6038453 reads processed; of these:
    509 ( 0.01%) short reads filtered out after trimming by size control
     17 ( 0.00%) empty reads filtered out after trimming by size control
6037927 (99.99%) reads available; of these:
 287050 ( 4.75%) trimmed reads available after processing
5750877 (95.25%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    125	  0.00%
 19	    198	  0.00%
 20	    334	  0.01%
 21	    515	  0.01%
 22	    987	  0.02%
 23	   1049	  0.02%
 24	    988	  0.02%
 25	   1220	  0.02%
 26	    896	  0.01%
 27	   1258	  0.02%
 28	   1420	  0.02%
 29	   3017	  0.05%
 30	   1468	  0.02%
 31	   1635	  0.03%
 32	   1761	  0.03%
 33	   2069	  0.03%
 34	   2645	  0.04%
 35	   5588	  0.09%
 36	   4504	  0.07%
 37	   5104	  0.08%
 38	   4686	  0.08%
 39	   5044	  0.08%
 40	   5603	  0.09%
 41	   5936	  0.10%
 42	   7491	  0.12%
 43	  11016	  0.18%
 44	  15011	  0.25%
 45	  20159	  0.33%
 46	  27215	  0.45%
 47	  35745	  0.59%
 48	  56311	  0.93%
 49	  56052	  0.93%
 50	5750877	 95.25%
6037927 reads passed initial QC


criterion=sequence-density
sequence-density=89.99
sequence-density-rank=1
fanout-score=17.66
fanout-score-rank=1
prefix-density=91.52
prefix-fanout=17.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGCC


criterion=fanout-score
sequence-density=89.99
sequence-density-rank=1
fanout-score=17.66
fanout-score-rank=1
prefix-density=91.52
prefix-fanout=17.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGCC -o SRR5031064 -
Input file:	STDIN
trimmed:	SRR5031064-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:46 2020 >> started

Wed Mar  4 07:17:55 2020 >> done (9.350s)
5903751 reads processed; of these:
 203573 ( 3.45%) short reads filtered out after trimming by size control
  21961 ( 0.37%) empty reads filtered out after trimming by size control
5678217 (96.18%) reads available; of these:
5495937 (96.79%) trimmed reads available after processing
 182280 ( 3.21%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  28973	  0.51%
 19	  58291	  1.03%
 20	 253403	  4.46%
 21	 657937	 11.59%
 22	2133427	 37.57%
 23	1033779	 18.21%
 24	 359015	  6.32%
 25	  64638	  1.14%
 26	  31411	  0.55%
 27	  30242	  0.53%
 28	  32977	  0.58%
 29	  27699	  0.49%
 30	  48573	  0.86%
 31	  68308	  1.20%
 32	  65824	  1.16%
 33	 168976	  2.98%
 34	  55121	  0.97%
 35	  34272	  0.60%
 36	  36068	  0.64%
 37	  23364	  0.41%
 38	  26995	  0.48%
 39	  24725	  0.44%
 40	  35233	  0.62%
 41	  44058	  0.78%
 42	  47042	  0.83%
 43	  34880	  0.61%
 44	  29772	  0.52%
 45	  31573	  0.56%
 46	  23753	  0.42%
 47	  18592	  0.33%
 48	   2310	  0.04%
 49	   3264	  0.06%
 50	 143722	  2.53%


criterion=sequence-density
sequence-density=3.47
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=21
prefix-density=0.01
prefix-fanout=1.0
sequence=TGTAAACATCCT


criterion=fanout-score
sequence-density=0.43
sequence-density-rank=30
fanout-score=14.80
fanout-score-rank=1
prefix-density=6.33
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 07:18:18
                             Started mapping on |	Mar 04 07:18:18
                                    Finished on |	Mar 04 07:19:05
       Mapping speed, Million of reads per hour |	445.20

                          Number of input reads |	5812393
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2773647
                        Uniquely mapped reads % |	47.72%
                          Average mapped length |	24.04
                       Number of splices: Total |	2983
            Number of splices: Annotated (sjdb) |	295
                       Number of splices: GT/AG |	2768
                       Number of splices: GC/AG |	25
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	188
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.63
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2439977
             % of reads mapped to multiple loci |	41.98%
        Number of reads mapped to too many loci |	118148
             % of reads mapped to too many loci |	2.03%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.64%
                     % of reads unmapped: other |	0.63%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	598769	598769	598769
N_multimapping	2439977	2439977	2439977
N_noFeature	131974	157057	2119288
N_ambiguous	1502419	952302	20286
UnstrandedReadsAssigned:1139254 PositiveStrandReadsAssigned:1664288 NegativeStrandReadsAssigned:634073
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031064 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031064-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,812,393 reads, 1,159,208 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 653 rounds

 109283 SRR5031064.ke.tsv
  52636 SRR5031064.se.tsv
 161919 total
==> SRR5031064.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031064.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031064 completed mapping pipeline successfully
