Starting /dee2/code/volunteer_pipeline.sh SRR5031017
    current disk space = 160553795584
    free memory = 73936372 
SRR5031017 SRAfilesize
69685aeb075703446a8d0f73549ed296  SRR5031017.sra
SRR5031017.sra file validated
SRR5031017 is single end
SRR5031017 is conventional basespace
SRR5031017 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37975	33.0	31.0	34.0	31.0	34.0
2	32.599	34.0	31.0	34.0	31.0	34.0
3	32.516	34.0	31.0	34.0	31.0	34.0
4	36.18	37.0	35.0	37.0	35.0	37.0
5	36.17325	37.0	35.0	37.0	35.0	37.0
6	35.885	37.0	35.0	37.0	35.0	37.0
7	35.96125	37.0	35.0	37.0	35.0	37.0
8	36.07375	37.0	35.0	37.0	35.0	37.0
9	37.724	39.0	37.0	39.0	35.0	39.0
10	37.632	39.0	37.0	39.0	35.0	39.0
11	37.242	39.0	37.0	39.0	33.0	39.0
12	37.62275	39.0	37.0	39.0	35.0	39.0
13	37.67925	39.0	37.0	39.0	35.0	39.0
14	39.192	41.0	39.0	41.0	36.0	41.0
15	38.9995	40.0	38.0	41.0	35.0	41.0
16	39.037	40.0	38.0	41.0	36.0	41.0
17	39.14825	40.0	38.0	41.0	36.0	41.0
18	39.001	41.0	38.0	41.0	35.0	41.0
19	38.89	40.0	38.0	41.0	35.0	41.0
20	38.7835	40.0	38.0	41.0	35.0	41.0
21	38.68975	40.0	38.0	41.0	34.0	41.0
22	38.5575	40.0	38.0	41.0	34.0	41.0
23	37.8095	40.0	37.0	41.0	32.0	41.0
24	38.37225	40.0	38.0	41.0	34.0	41.0
25	38.74625	40.0	38.0	41.0	35.0	41.0
26	38.81075	40.0	38.0	41.0	35.0	41.0
27	38.9675	40.0	39.0	41.0	36.0	41.0
28	38.893	40.0	38.0	41.0	35.0	41.0
29	38.59675	40.0	38.0	41.0	34.0	41.0
30	38.58375	40.0	38.0	41.0	34.0	41.0
31	38.83525	40.0	38.0	41.0	35.0	41.0
32	38.7545	40.0	38.0	41.0	35.0	41.0
33	38.476	40.0	38.0	41.0	34.0	41.0
34	38.432	40.0	38.0	41.0	34.0	41.0
35	38.2315	40.0	38.0	41.0	33.0	41.0
36	38.241	40.0	38.0	41.0	34.0	41.0
37	37.6895	40.0	37.0	41.0	32.0	41.0
38	37.66525	40.0	37.0	41.0	32.0	41.0
39	37.9445	40.0	38.0	41.0	33.0	41.0
40	37.9765	40.0	38.0	41.0	33.0	41.0
41	38.19675	40.0	38.0	41.0	33.0	41.0
42	38.222	40.0	38.0	41.0	34.0	41.0
43	38.0875	40.0	38.0	41.0	33.0	41.0
44	38.22825	40.0	38.0	41.0	34.0	41.0
45	38.17	40.0	38.0	41.0	33.0	41.0
46	37.904	40.0	38.0	41.0	33.0	41.0
47	37.308	40.0	37.0	41.0	31.0	41.0
48	36.82575	40.0	36.0	41.0	31.0	41.0
49	37.0335	40.0	36.0	41.0	31.0	41.0
50	36.4725	39.0	36.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	3.0
19	1.0
20	4.0
21	7.0
22	6.0
23	4.0
24	2.0
25	8.0
26	16.0
27	20.0
28	31.0
29	26.0
30	47.0
31	49.0
32	85.0
33	98.0
34	121.0
35	183.0
36	276.0
37	446.0
38	712.0
39	1852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.0	22.025	56.225	13.750000000000002
2	25.313597591570495	25.639739086803814	22.70446562970396	26.342197691921726
3	17.275	18.675	21.375	42.675000000000004
4	27.975	36.199999999999996	13.125	22.7
5	21.875	38.775	26.825	12.525
6	34.150000000000006	16.05	35.225	14.575
7	12.85	24.625	25.324999999999996	37.2
8	23.45	39.35	15.725	21.475
9	19.125	37.075	30.375000000000004	13.425
10	20.625	22.400000000000002	25.575	31.4
11	30.8	11.65	15.0	42.55
12	17.724999999999998	17.325	24.375	40.575
13	20.25	21.9	37.25	20.599999999999998
14	37.824999999999996	22.175	24.375	15.625
15	37.75	23.674999999999997	26.974999999999998	11.600000000000001
16	14.424999999999999	25.624999999999996	23.125	36.825
17	24.474999999999998	23.5	37.724999999999994	14.299999999999999
18	27.825	19.225	27.975	24.975
19	39.800000000000004	13.600000000000001	28.625	17.974999999999998
20	48.225	16.825000000000003	23.275000000000002	11.675
21	22.5	26.174999999999997	23.275000000000002	28.050000000000004
22	33.0	12.225	46.6	8.175
23	24.25	12.425	48.55	14.774999999999999
24	53.65	13.15	27.224999999999998	5.975
25	55.25	22.825	14.149999999999999	7.775
26	29.349999999999998	49.15	11.924999999999999	9.575
27	13.075000000000001	53.125	21.75	12.049999999999999
28	8.9	25.95	51.824999999999996	13.325000000000001
29	10.674999999999999	11.5	59.650000000000006	18.175
30	9.5	5.475	37.55	47.475
31	13.15	4.475	48.199999999999996	34.175
32	25.924999999999997	5.075	23.200000000000003	45.800000000000004
33	55.45	7.074999999999999	13.3	24.175
34	67.35	6.525	12.950000000000001	13.175
35	68.15	5.825	17.125	8.9
36	41.575	7.6499999999999995	39.800000000000004	10.975
37	46.375	10.424999999999999	21.675	21.525
38	24.25	14.274999999999999	11.65	49.825
39	14.499999999999998	21.275	8.924999999999999	55.300000000000004
40	12.775	50.949999999999996	8.75	27.525
41	22.025	55.025	7.875	15.075
42	51.4	30.625000000000004	9.575	8.4
43	56.974999999999994	26.05	7.55	9.425
44	31.324999999999996	50.349999999999994	6.7	11.625
45	16.0	54.50000000000001	11.65	17.849999999999998
46	11.35	27.325	17.175	44.15
47	9.2	12.725	41.349999999999994	36.725
48	10.174999999999999	10.125	22.325	57.375
49	10.625	18.6	12.525	58.25
50	17.549999999999997	40.0	10.475	31.974999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	2.0
23	1.0
24	0.0
25	1.0
26	2.0
27	2.0
28	2.0
29	3.0
30	4.0
31	9.5
32	15.0
33	13.0
34	11.0
35	14.5
36	18.0
37	23.0
38	28.0
39	33.5
40	39.0
41	49.0
42	59.0
43	88.0
44	117.0
45	163.0
46	209.0
47	405.0
48	601.0
49	607.5
50	614.0
51	777.5
52	941.0
53	655.5
54	370.0
55	361.0
56	352.0
57	319.5
58	287.0
59	181.0
60	75.0
61	64.0
62	53.0
63	75.0
64	97.0
65	60.5
66	24.0
67	15.0
68	6.0
69	10.5
70	15.0
71	34.0
72	53.0
73	28.0
74	3.0
75	1.5
76	0.0
77	1.0
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.35000000000000003
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.675000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.14178595773687	27.925
2	9.134287661895025	6.7
3	4.158145875937287	4.575
4	2.044989775051125	3.0
5	1.3633265167007498	2.5
6	0.408997955010225	0.8999999999999999
7	1.4314928425357873	3.675
8	0.8861622358554875	2.6
9	0.408997955010225	1.35
>10	3.54464894342195	25.650000000000002
>50	0.27266530334015	7.55
>100	0.2044989775051125	13.575000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	258	6.45	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	176	4.3999999999999995	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	109	2.725	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	97	2.4250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	67	1.675	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	49	1.225	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	31	0.775	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	25	0.625	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC	19	0.475	RNA PCR Primer, Index 37 (100% over 44bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	13	0.325	RNA PCR Primer, Index 2 (100% over 35bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	12	0.3	No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	11	0.27499999999999997	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC	11	0.27499999999999997	RNA PCR Primer, Index 37 (100% over 50bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	9	0.22499999999999998	No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	8	0.2	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	8	0.2	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	8	0.2	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTGTGGAAT	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	7	0.17500000000000002	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTAT	5	0.125	RNA PCR Primer, Index 37 (100% over 48bp)
TACCACAGGGTAGAACCACGGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGATGATGAGAGCTTTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCAGGCTCAGTCCCCTCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG	5	0.125	RNA PCR Primer, Index 7 (97% over 36bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.325	0.0	0.0	0.0
2	0.0	0.325	0.0	0.0	0.0
3	0.0	0.5	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.6	0.0	0.0	0.0
7	0.0	1.175	0.0	0.0	0.0
8	0.0	1.325	0.0	0.0	0.0
9	0.0	1.35	0.0	0.0	0.0
10	0.0	1.45	0.0	0.0	0.0
11	0.0	1.55	0.0	0.0	0.0
12	0.0	1.9	0.0	0.0	0.0
13	0.0	2.15	0.0	0.0	0.0
14	0.0	2.4	0.0	0.0	0.0
15	0.0	2.9	0.0	0.0	0.0
16	0.0	3.625	0.0	0.0	0.0
17	0.0	4.175	0.0	0.0	0.0
18	0.0	5.15	0.0	0.0	0.0
19	0.0	5.775	0.0	0.0	0.0
20	0.0	7.325	0.0	0.0	0.0
21	0.0	11.55	0.0	0.0	0.0
22	0.0	22.575	0.0	0.0	0.0
23	0.0	55.85	0.0	0.0	0.0
24	0.0	71.6	0.0	0.0	0.0
25	0.0	77.225	0.0	0.0	0.0
26	0.0	78.15	0.0	0.0	0.0
27	0.0	78.575	0.0	0.0	0.0
28	0.0	79.175	0.0	0.0	0.0
29	0.0	79.625	0.0	0.0	0.0
30	0.0	79.925	0.0	0.0	0.0
31	0.0	80.6	0.0	0.0	0.0
32	0.0	81.625	0.0	0.0	0.0
33	0.0	82.725	0.0	0.0	0.0
34	0.0	85.425	0.0	0.0	0.0
35	0.0	86.475	0.0	0.0	0.0
36	0.0	87.075	0.0	0.0	0.0
37	0.0	88.0	0.0	0.0	0.0
38	0.0	88.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTAAT	40	5.315087E-9	44.000004	4
TCAAGTA	40	5.315087E-9	44.000004	2
AATCCAG	40	5.315087E-9	44.000004	8
ATCCAGG	40	5.315087E-9	44.000004	9
CTTTAAC	20	6.952051E-4	44.000004	12
GAGTGGA	20	6.952051E-4	44.000004	20
AGGATAG	40	5.315087E-9	44.000004	13
ATAGGCT	40	5.315087E-9	44.000004	16
AGTGGAA	20	6.952051E-4	44.000004	21
GAGCTTG	20	6.952051E-4	44.000004	13
GGATCCG	20	6.952051E-4	44.000004	3
CCGTCTG	20	6.952051E-4	44.000004	7
TCGGATC	20	6.952051E-4	44.000004	1
CGGATCC	20	6.952051E-4	44.000004	2
TCCAGGA	40	5.315087E-9	44.000004	10
GTGAGTG	20	6.952051E-4	44.000004	18
GGATAGG	40	5.315087E-9	44.000004	14
AGTAATC	40	5.315087E-9	44.000004	5
GATCCGT	20	6.952051E-4	44.000004	4
ACCCTTT	20	6.952051E-4	44.000004	9
>>END_MODULE
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
Read 284172 spots for SRR5031017.sra
Written 284172 spots for SRR5031017.sra
Read 284171 spots for SRR5031017.sra
Written 284171 spots for SRR5031017.sra
SRR ids: ['SRR5031017.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hv12w6fu
SRR5031017.sra spots: 3410053
blocks: [[1, 284171], [284172, 568342], [568343, 852513], [852514, 1136684], [1136685, 1420855], [1420856, 1705026], [1705027, 1989197], [1989198, 2273368], [2273369, 2557539], [2557540, 2841710], [2841711, 3125881], [3125882, 3410053]]
SRR5031017 file size 589973
SRR5031017 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031017 SRR5031017_1.fastq
Input file:	SRR5031017_1.fastq
trimmed:	SRR5031017-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:06:59 2020 >> started

Wed Mar  4 07:07:01 2020 >> done (2.169s)
3410053 reads processed; of these:
    227 ( 0.01%) short reads filtered out after trimming by size control
      2 ( 0.00%) empty reads filtered out after trimming by size control
3409824 (99.99%) reads available; of these:
 192412 ( 5.64%) trimmed reads available after processing
3217412 (94.36%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     57	  0.00%
 19	    101	  0.00%
 20	    195	  0.01%
 21	    240	  0.01%
 22	    403	  0.01%
 23	    442	  0.01%
 24	    433	  0.01%
 25	    598	  0.02%
 26	    484	  0.01%
 27	    627	  0.02%
 28	    790	  0.02%
 29	   1360	  0.04%
 30	    831	  0.02%
 31	    852	  0.02%
 32	   1104	  0.03%
 33	   1248	  0.04%
 34	   1532	  0.04%
 35	   2773	  0.08%
 36	   2368	  0.07%
 37	   2758	  0.08%
 38	   2835	  0.08%
 39	   2991	  0.09%
 40	   3358	  0.10%
 41	   3762	  0.11%
 42	   4737	  0.14%
 43	   7100	  0.21%
 44	  10527	  0.31%
 45	  14214	  0.42%
 46	  18948	  0.56%
 47	  23939	  0.70%
 48	  38414	  1.13%
 49	  42391	  1.24%
 50	3217412	 94.36%
3409824 reads passed initial QC


criterion=sequence-density
sequence-density=88.58
sequence-density-rank=1
fanout-score=18.75
fanout-score-rank=1
prefix-density=90.31
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC


criterion=fanout-score
sequence-density=88.58
sequence-density-rank=1
fanout-score=18.75
fanout-score-rank=1
prefix-density=90.31
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC -o SRR5031017 -
Input file:	STDIN
trimmed:	SRR5031017-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:07:18 2020 >> started

Wed Mar  4 07:07:24 2020 >> done (5.578s)
3333199 reads processed; of these:
 156426 ( 4.69%) short reads filtered out after trimming by size control
  11046 ( 0.33%) empty reads filtered out after trimming by size control
3165727 (94.98%) reads available; of these:
3040316 (96.04%) trimmed reads available after processing
 125411 ( 3.96%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  17270	  0.55%
 19	  38886	  1.23%
 20	 158233	  5.00%
 21	 376076	 11.88%
 22	1103047	 34.84%
 23	 548942	 17.34%
 24	 202765	  6.41%
 25	  34437	  1.09%
 26	  17942	  0.57%
 27	  17903	  0.57%
 28	  19963	  0.63%
 29	  16223	  0.51%
 30	  28213	  0.89%
 31	  36770	  1.16%
 32	  39962	  1.26%
 33	  93599	  2.96%
 34	  35572	  1.12%
 35	  20275	  0.64%
 36	  22434	  0.71%
 37	  16058	  0.51%
 38	  17224	  0.54%
 39	  15893	  0.50%
 40	  22304	  0.70%
 41	  28922	  0.91%
 42	  31301	  0.99%
 43	  23807	  0.75%
 44	  20623	  0.65%
 45	  23431	  0.74%
 46	  17518	  0.55%
 47	  13385	  0.42%
 48	   1956	  0.06%
 49	   2907	  0.09%
 50	 101886	  3.22%


criterion=sequence-density
sequence-density=5.32
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCTTTAACCTGTGAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=49
fanout-score=26.45
fanout-score-rank=1
prefix-density=0.14
prefix-fanout=1.0
sequence=TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT
                                 Started job on |	Mar 04 07:07:46
                             Started mapping on |	Mar 04 07:07:46
                                    Finished on |	Mar 04 07:08:13
       Mapping speed, Million of reads per hour |	432.31

                          Number of input reads |	3242352
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1553332
                        Uniquely mapped reads % |	47.91%
                          Average mapped length |	24.28
                       Number of splices: Total |	1872
            Number of splices: Annotated (sjdb) |	234
                       Number of splices: GT/AG |	1723
                       Number of splices: GC/AG |	16
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	131
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.60
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1309433
             % of reads mapped to multiple loci |	40.39%
        Number of reads mapped to too many loci |	78660
             % of reads mapped to too many loci |	2.43%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.52%
                     % of reads unmapped: other |	0.76%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	379587	379587	379587
N_multimapping	1309433	1309433	1309433
N_noFeature	66192	81205	1178350
N_ambiguous	829360	510703	12200
UnstrandedReadsAssigned:657780 PositiveStrandReadsAssigned:961424 NegativeStrandReadsAssigned:362782
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031017 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031017-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,242,352 reads, 630,840 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 668 rounds

 109283 SRR5031017.ke.tsv
  52636 SRR5031017.se.tsv
 161919 total
==> SRR5031017.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031017.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031017 completed mapping pipeline successfully
