Starting /dee2/code/volunteer_pipeline.sh SRR5030899
    current disk space = 160663519232
    free memory = 73950808 
SRR5030899 SRAfilesize
6305b98888e448e4dee2f219f5022857  SRR5030899.sra
SRR5030899.sra file validated
SRR5030899 is single end
SRR5030899 is conventional basespace
SRR5030899 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030899_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39075	33.0	31.0	34.0	31.0	34.0
2	32.73875	34.0	31.0	34.0	31.0	34.0
3	32.819	34.0	31.0	34.0	31.0	34.0
4	36.22625	37.0	37.0	37.0	35.0	37.0
5	36.329	37.0	37.0	37.0	35.0	37.0
6	36.077	37.0	35.0	37.0	35.0	37.0
7	36.05275	37.0	35.0	37.0	35.0	37.0
8	36.14625	37.0	36.0	37.0	35.0	37.0
9	37.84575	39.0	38.0	39.0	35.0	39.0
10	37.90625	39.0	38.0	39.0	35.0	39.0
11	37.84	39.0	38.0	39.0	35.0	39.0
12	37.8615	39.0	38.0	39.0	35.0	39.0
13	37.78575	39.0	38.0	39.0	35.0	39.0
14	39.283	41.0	39.0	41.0	36.0	41.0
15	39.38125	41.0	39.0	41.0	36.0	41.0
16	39.19025	40.0	39.0	41.0	36.0	41.0
17	39.46175	41.0	39.0	41.0	36.0	41.0
18	39.321	41.0	39.0	41.0	36.0	41.0
19	38.95525	40.0	38.0	41.0	35.0	41.0
20	39.13825	40.0	39.0	41.0	35.0	41.0
21	39.17475	40.0	39.0	41.0	36.0	41.0
22	39.23975	41.0	39.0	41.0	36.0	41.0
23	39.054	40.0	39.0	41.0	36.0	41.0
24	38.80925	40.0	38.0	41.0	35.0	41.0
25	39.0825	41.0	39.0	41.0	36.0	41.0
26	38.99525	40.0	39.0	41.0	35.0	41.0
27	38.993	40.0	39.0	41.0	35.0	41.0
28	38.85675	40.0	39.0	41.0	35.0	41.0
29	38.3515	40.0	38.0	41.0	33.0	41.0
30	38.6995	40.0	38.0	41.0	34.0	41.0
31	38.8905	41.0	39.0	41.0	35.0	41.0
32	38.9475	40.0	39.0	41.0	35.0	41.0
33	38.161	40.0	38.0	41.0	33.0	41.0
34	38.17825	40.0	38.0	41.0	34.0	41.0
35	37.87225	40.0	38.0	40.0	33.0	41.0
36	33.97525	38.0	31.0	40.0	23.0	41.0
37	35.76125	38.0	35.0	40.0	30.0	41.0
38	37.04225	39.0	37.0	40.0	31.0	41.0
39	37.03775	39.0	37.0	40.0	31.0	41.0
40	37.0135	39.0	37.0	41.0	31.0	41.0
41	37.1165	39.0	37.0	41.0	31.0	41.0
42	37.37825	40.0	37.0	41.0	32.0	41.0
43	37.06875	40.0	37.0	41.0	31.0	41.0
44	36.9845	40.0	37.0	41.0	32.0	41.0
45	36.781	39.0	36.0	41.0	31.0	41.0
46	35.033	38.0	34.0	40.0	25.0	41.0
47	35.7885	39.0	35.0	40.0	28.0	41.0
48	36.0005	39.0	36.0	41.0	30.0	41.0
49	35.0115	39.0	35.0	40.0	27.0	41.0
50	30.12475	34.0	27.0	39.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	1.0
17	0.0
18	3.0
19	3.0
20	5.0
21	5.0
22	3.0
23	12.0
24	13.0
25	12.0
26	22.0
27	27.0
28	37.0
29	39.0
30	60.0
31	64.0
32	88.0
33	107.0
34	144.0
35	183.0
36	288.0
37	490.0
38	1015.0
39	1377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.917835671342687	21.8436873747495	55.16032064128257	14.07815631262525
2	23.95	27.35	21.725	26.974999999999998
3	19.45	17.825	19.85	42.875
4	25.974999999999998	35.875	13.750000000000002	24.4
5	21.125	38.425	28.125	12.325
6	36.025	14.924999999999999	37.925	11.125
7	13.900000000000002	24.55	25.35	36.199999999999996
8	24.675	41.575	12.85	20.9
9	19.7	38.324999999999996	29.7	12.275
10	21.0	19.875	27.175	31.95
11	30.475	13.675	13.5	42.35
12	15.525	17.075000000000003	24.125	43.275000000000006
13	21.525	20.974999999999998	37.15	20.349999999999998
14	36.975	24.6	26.174999999999997	12.25
15	38.125	22.425	27.725	11.725
16	11.799999999999999	27.35	20.875	39.975
17	23.925	23.549999999999997	37.95	14.575
18	27.900000000000002	18.65	30.45	23.0
19	37.75	14.374999999999998	30.55	17.325
20	49.3	17.175	21.675	11.85
21	21.15	25.874999999999996	24.224999999999998	28.749999999999996
22	31.374999999999996	12.174999999999999	48.9	7.55
23	25.1	12.975	49.9	12.025
24	52.525	12.6	28.050000000000004	6.825
25	55.574999999999996	22.0	13.15	9.275
26	28.9	49.3	12.5	9.3
27	13.750000000000002	53.7	22.775000000000002	9.775
28	10.2	26.55	51.575	11.675
29	8.525	11.325000000000001	61.275	18.875
30	9.1	5.8999999999999995	38.574999999999996	46.425
31	12.3	4.9	46.425	36.375
32	25.3	5.375	24.125	45.2
33	55.85	7.1	11.75	25.3
34	68.425	6.45	12.950000000000001	12.174999999999999
35	67.75	6.4	17.65	8.200000000000001
36	45.675	7.324999999999999	36.15	10.85
37	45.275	9.775	22.95	22.0
38	25.35	13.5	10.9	50.24999999999999
39	12.975	23.25	7.9	55.875
40	13.475000000000001	50.7	9.575	26.25
41	23.0	55.175	8.125	13.700000000000001
42	52.6	30.725	8.05	8.625
43	56.89999999999999	28.249999999999996	6.925000000000001	7.925
44	31.35	50.975	6.05	11.625
45	15.225	56.39999999999999	10.825	17.549999999999997
46	10.95	28.425	17.9	42.725
47	7.85	13.575000000000001	40.425	38.15
48	8.475000000000001	11.675	23.025000000000002	56.825
49	12.525	19.125	11.0	57.35
50	20.125	38.4	10.95	30.525000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	1.0
25	1.5
26	2.0
27	1.5
28	1.0
29	1.5
30	2.0
31	6.0
32	10.0
33	13.5
34	17.0
35	22.5
36	28.0
37	48.0
38	68.0
39	57.0
40	46.0
41	48.0
42	50.0
43	90.5
44	131.0
45	175.0
46	219.0
47	386.0
48	553.0
49	570.5
50	588.0
51	769.0
52	950.0
53	649.0
54	348.0
55	359.5
56	371.0
57	353.5
58	336.0
59	205.0
60	74.0
61	62.5
62	51.0
63	63.0
64	75.0
65	47.0
66	19.0
67	15.0
68	11.0
69	11.5
70	12.0
71	21.0
72	30.0
73	15.5
74	1.0
75	1.0
76	1.0
77	1.5
78	2.0
79	1.0
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.824999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53668208856577	28.95
2	8.06345009914078	6.1
3	4.49438202247191	5.1
4	2.0489094514210175	3.1
5	1.5862524785194978	3.0
6	1.4540647719762063	3.3000000000000003
7	0.7270323859881032	1.925
8	0.7931262392597489	2.4
9	0.3304692663582287	1.125
>10	3.56906807666887	26.200000000000003
>50	0.13218770654329146	3.3000000000000003
>100	0.2643754130865829	15.5
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	217	5.425	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	174	4.35	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	110	2.75	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	76	1.9	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	56	1.4000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	38	0.95	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	38	0.95	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	30	0.75	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	30	0.75	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG	27	0.675	RNA PCR Primer, Index 2 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	23	0.575	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	12	0.3	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	11	0.27499999999999997	No Hit
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC	11	0.27499999999999997	RNA PCR Primer, Index 24 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTC	8	0.2	RNA PCR Primer, Index 24 (100% over 44bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	8	0.2	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	8	0.2	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	8	0.2	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	7	0.17500000000000002	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	6	0.15	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	6	0.15	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.3	0.0	0.0	0.0
2	0.0	0.3	0.0	0.0	0.0
3	0.0	0.3	0.0	0.0	0.0
4	0.0	0.35	0.0	0.0	0.0
5	0.0	0.375	0.0	0.0	0.0
6	0.0	0.45	0.0	0.0	0.0
7	0.0	0.75	0.0	0.0	0.0
8	0.0	0.9	0.0	0.0	0.0
9	0.0	0.975	0.0	0.0	0.0
10	0.0	1.05	0.0	0.0	0.0
11	0.0	1.225	0.0	0.0	0.0
12	0.0	1.525	0.0	0.0	0.0
13	0.0	1.775	0.0	0.0	0.0
14	0.0	1.95	0.0	0.0	0.0
15	0.0	2.2	0.0	0.0	0.0
16	0.0	3.35	0.0	0.0	0.0
17	0.0	3.85	0.0	0.0	0.0
18	0.0	4.725	0.0	0.0	0.0
19	0.0	5.175	0.0	0.0	0.0
20	0.0	6.225	0.0	0.0	0.0
21	0.0	10.825	0.0	0.0	0.0
22	0.0	22.4	0.0	0.0	0.0
23	0.0	55.275	0.0	0.0	0.0
24	0.0	71.625	0.0	0.0	0.0
25	0.0	76.275	0.0	0.0	0.0
26	0.0	77.575	0.0	0.0	0.0
27	0.0	78.0	0.0	0.0	0.0
28	0.0	78.55	0.0	0.0	0.0
29	0.0	78.9	0.0	0.0	0.0
30	0.0	79.25	0.0	0.0	0.0
31	0.0	79.9	0.0	0.0	0.0
32	0.0	81.125	0.0	0.0	0.0
33	0.0	82.125	0.0	0.0	0.0
34	0.0	83.875	0.0	0.0	0.0
35	0.0	84.95	0.0	0.0	0.0
36	0.0	85.275	0.0	0.0	0.0
37	0.0	85.65	0.0	0.0	0.0
38	0.0	85.975	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCG	20	6.952051E-4	44.000004	8
TCCTCGA	20	6.952051E-4	44.000004	9
TCGACTG	20	6.952051E-4	44.000004	12
TTGTATA	20	6.952051E-4	44.000004	13
TGCGTGG	20	6.952051E-4	44.000004	19
CATCCTC	20	6.952051E-4	44.000004	7
GAAGCTT	20	6.952051E-4	44.000004	19
CCTTGCG	20	6.952051E-4	44.000004	16
ACCTGTT	20	6.952051E-4	44.000004	17
ATAGTTT	20	6.952051E-4	44.000004	17
AGGCTTG	20	6.952051E-4	44.000004	18
GGAAGCT	20	6.952051E-4	44.000004	18
CTTGCGT	20	6.952051E-4	44.000004	17
TTGCGTG	20	6.952051E-4	44.000004	18
TGGAAGC	20	6.952051E-4	44.000004	17
TGTATAG	20	6.952051E-4	44.000004	14
AAGCTTG	20	6.952051E-4	44.000004	20
TATAGTT	20	6.952051E-4	44.000004	16
ACTGTTG	30	1.9515464E-6	44.0	18
TAGAACC	30	1.9515464E-6	44.0	7
>>END_MODULE
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285710 spots for SRR5030899.sra
Written 285710 spots for SRR5030899.sra
Read 285718 spots for SRR5030899.sra
Written 285718 spots for SRR5030899.sra
SRR ids: ['SRR5030899.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6y0w8ksr
SRR5030899.sra spots: 3428528
blocks: [[1, 285710], [285711, 571420], [571421, 857130], [857131, 1142840], [1142841, 1428550], [1428551, 1714260], [1714261, 1999970], [1999971, 2285680], [2285681, 2571390], [2571391, 2857100], [2857101, 3142810], [3142811, 3428528]]
SRR5030899 file size 593163
SRR5030899 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030899 SRR5030899_1.fastq
Input file:	SRR5030899_1.fastq
trimmed:	SRR5030899-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:40:07 2020 >> started

Wed Mar  4 06:40:09 2020 >> done (2.175s)
3428528 reads processed; of these:
    158 ( 0.00%) short reads filtered out after trimming by size control
     11 ( 0.00%) empty reads filtered out after trimming by size control
3428359 (100.00%) reads available; of these:
 198575 ( 5.79%) trimmed reads available after processing
3229784 (94.21%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     42	  0.00%
 19	     59	  0.00%
 20	    144	  0.00%
 21	    167	  0.00%
 22	    264	  0.01%
 23	    338	  0.01%
 24	    315	  0.01%
 25	    416	  0.01%
 26	    356	  0.01%
 27	    478	  0.01%
 28	    605	  0.02%
 29	    999	  0.03%
 30	    696	  0.02%
 31	    969	  0.03%
 32	    928	  0.03%
 33	   1133	  0.03%
 34	   3336	  0.10%
 35	   2580	  0.08%
 36	   1848	  0.05%
 37	   2123	  0.06%
 38	   2370	  0.07%
 39	   2683	  0.08%
 40	   3164	  0.09%
 41	   3458	  0.10%
 42	   5137	  0.15%
 43	   6320	  0.18%
 44	  11534	  0.34%
 45	  14544	  0.42%
 46	  13032	  0.38%
 47	  19498	  0.57%
 48	  41444	  1.21%
 49	  57595	  1.68%
 50	3229784	 94.21%
3428359 reads passed initial QC


criterion=sequence-density
sequence-density=86.09
sequence-density-rank=1
fanout-score=18.77
fanout-score-rank=1
prefix-density=87.70
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGCC


criterion=fanout-score
sequence-density=86.09
sequence-density-rank=1
fanout-score=18.77
fanout-score-rank=1
prefix-density=87.70
prefix-fanout=18.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGCC -o SRR5030899 -
Input file:	STDIN
trimmed:	SRR5030899-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:40:25 2020 >> started

Wed Mar  4 06:40:30 2020 >> done (4.417s)
3349546 reads processed; of these:
 150531 ( 4.49%) short reads filtered out after trimming by size control
  10301 ( 0.31%) empty reads filtered out after trimming by size control
3188714 (95.20%) reads available; of these:
2941839 (92.26%) trimmed reads available after processing
 246875 ( 7.74%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  19039	  0.60%
 19	  37674	  1.18%
 20	 161684	  5.07%
 21	 382256	 11.99%
 22	1125291	 35.29%
 23	 545686	 17.11%
 24	 183036	  5.74%
 25	  32705	  1.03%
 26	  16238	  0.51%
 27	  15339	  0.48%
 28	  17172	  0.54%
 29	  13706	  0.43%
 30	  24836	  0.78%
 31	  33716	  1.06%
 32	  30813	  0.97%
 33	  56769	  1.78%
 34	  28356	  0.89%
 35	  20710	  0.65%
 36	  22476	  0.70%
 37	  12263	  0.38%
 38	  13827	  0.43%
 39	  13205	  0.41%
 40	  17495	  0.55%
 41	  24136	  0.76%
 42	  25722	  0.81%
 43	  19603	  0.61%
 44	  16632	  0.52%
 45	  19989	  0.63%
 46	  15334	  0.48%
 47	  12189	  0.38%
 48	   2702	  0.08%
 49	   9271	  0.29%
 50	 218844	  6.86%


criterion=sequence-density
sequence-density=10.24
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.45
sequence-density-rank=32
fanout-score=16.18
fanout-score-rank=1
prefix-density=7.19
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:40:49
                             Started mapping on |	Mar 04 06:40:49
                                    Finished on |	Mar 04 06:41:14
       Mapping speed, Million of reads per hour |	470.52

                          Number of input reads |	3267527
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1666398
                        Uniquely mapped reads % |	51.00%
                          Average mapped length |	25.85
                       Number of splices: Total |	1463
            Number of splices: Annotated (sjdb) |	225
                       Number of splices: GT/AG |	1326
                       Number of splices: GC/AG |	10
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	127
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.61
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1304782
             % of reads mapped to multiple loci |	39.93%
        Number of reads mapped to too many loci |	61997
             % of reads mapped to too many loci |	1.90%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.61%
                     % of reads unmapped: other |	0.56%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	296347	296347	296347
N_multimapping	1304782	1304782	1304782
N_noFeature	77032	91010	1258423
N_ambiguous	905307	556659	14312
UnstrandedReadsAssigned:684059 PositiveStrandReadsAssigned:1018729 NegativeStrandReadsAssigned:393663
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030899 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030899-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,267,527 reads, 680,981 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,140 rounds

 109283 SRR5030899.ke.tsv
  52636 SRR5030899.se.tsv
 161919 total
==> SRR5030899.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030899.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030899 completed mapping pipeline successfully
