Starting /dee2/code/volunteer_pipeline.sh SRR5031022
    current disk space = 160536739840
    free memory = 73964368 
SRR5031022 SRAfilesize
90dab9dc4798012128270d33f7b292e3  SRR5031022.sra
SRR5031022.sra file validated
SRR5031022 is single end
SRR5031022 is conventional basespace
SRR5031022 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33625	33.0	31.0	34.0	31.0	34.0
2	32.60225	34.0	31.0	34.0	31.0	34.0
3	32.64125	34.0	31.0	34.0	31.0	34.0
4	36.2195	37.0	35.0	37.0	35.0	37.0
5	36.07975	37.0	35.0	37.0	35.0	37.0
6	35.9495	37.0	35.0	37.0	35.0	37.0
7	35.835	37.0	35.0	37.0	35.0	37.0
8	36.0085	37.0	35.0	37.0	35.0	37.0
9	37.62425	39.0	37.0	39.0	35.0	39.0
10	37.6585	39.0	37.0	39.0	35.0	39.0
11	37.2945	39.0	37.0	39.0	33.0	39.0
12	37.635	39.0	37.0	39.0	35.0	39.0
13	37.632	39.0	37.0	39.0	35.0	39.0
14	39.02125	40.0	38.0	41.0	36.0	41.0
15	38.88675	40.0	38.0	41.0	35.0	41.0
16	39.0945	40.0	38.0	41.0	36.0	41.0
17	39.2255	40.0	39.0	41.0	36.0	41.0
18	39.06425	41.0	38.0	41.0	35.0	41.0
19	38.86925	40.0	38.0	41.0	35.0	41.0
20	38.81275	40.0	38.0	41.0	35.0	41.0
21	38.903	40.0	38.0	41.0	35.0	41.0
22	38.8895	40.0	38.0	41.0	35.0	41.0
23	38.533	40.0	38.0	41.0	34.0	41.0
24	38.848	40.0	38.0	41.0	35.0	41.0
25	38.971	40.0	39.0	41.0	35.0	41.0
26	38.853	40.0	38.0	41.0	35.0	41.0
27	38.9585	40.0	38.0	41.0	35.0	41.0
28	38.675	40.0	38.0	41.0	34.0	41.0
29	38.212	40.0	38.0	41.0	33.0	41.0
30	38.17875	40.0	38.0	41.0	33.0	41.0
31	38.4175	40.0	38.0	41.0	34.0	41.0
32	38.4905	40.0	38.0	41.0	34.0	41.0
33	38.03625	40.0	38.0	41.0	33.0	41.0
34	38.18075	40.0	38.0	41.0	34.0	41.0
35	37.821	40.0	37.0	41.0	33.0	41.0
36	37.79375	40.0	37.0	41.0	33.0	41.0
37	37.179	39.0	37.0	41.0	31.0	41.0
38	37.152	39.0	36.0	40.0	31.0	41.0
39	37.6685	39.0	37.0	41.0	33.0	41.0
40	37.65075	40.0	37.0	41.0	33.0	41.0
41	37.87425	40.0	37.0	41.0	33.0	41.0
42	37.7795	40.0	37.0	41.0	33.0	41.0
43	37.76525	40.0	37.0	41.0	33.0	41.0
44	37.755	40.0	37.0	41.0	33.0	41.0
45	37.66475	40.0	37.0	41.0	33.0	41.0
46	37.4535	40.0	37.0	41.0	33.0	41.0
47	36.93	40.0	36.0	41.0	31.0	41.0
48	36.617	39.0	35.0	41.0	31.0	41.0
49	36.78075	39.0	35.0	41.0	31.0	41.0
50	36.1105	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	2.0
16	0.0
17	2.0
18	3.0
19	0.0
20	4.0
21	3.0
22	4.0
23	3.0
24	11.0
25	7.0
26	13.0
27	12.0
28	24.0
29	33.0
30	50.0
31	52.0
32	94.0
33	111.0
34	155.0
35	203.0
36	284.0
37	496.0
38	809.0
39	1624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.925	24.675	45.775	18.625
2	24.56184276414622	26.43965948923385	19.57936905358037	29.41912869303956
3	23.799999999999997	16.825000000000003	24.325	35.05
4	35.099999999999994	28.925	14.6	21.375
5	22.45	35.9	26.950000000000003	14.7
6	28.249999999999996	17.474999999999998	36.425000000000004	17.849999999999998
7	19.125	20.974999999999998	20.349999999999998	39.550000000000004
8	22.1	27.450000000000003	20.025000000000002	30.425
9	24.45	30.85	24.325	20.375
10	22.6	20.424999999999997	29.075	27.900000000000002
11	33.5	12.8	16.1	37.6
12	17.4	20.974999999999998	30.65	30.975
13	29.525000000000002	15.75	34.75	19.975
14	30.075000000000003	28.525	20.549999999999997	20.849999999999998
15	34.4	23.575	27.375	14.649999999999999
16	20.349999999999998	18.15	25.0	36.5
17	27.425	23.225	34.65	14.7
18	26.825	16.0	30.175	27.0
19	36.6	14.674999999999999	31.6	17.125
20	42.275	17.075000000000003	25.55	15.1
21	25.374999999999996	22.3	23.3	29.025000000000002
22	31.525	14.7	42.275	11.5
23	27.450000000000003	14.174999999999999	39.975	18.4
24	48.875	14.875	26.275	9.975000000000001
25	45.725	25.124999999999996	16.575	12.575
26	28.525	41.199999999999996	15.525	14.75
27	18.175	41.225	24.075	16.525000000000002
28	14.299999999999999	22.45	43.9	19.35
29	16.400000000000002	13.775	50.224999999999994	19.6
30	13.05	8.825	33.5	44.625
31	15.75	6.825	43.575	33.85
32	29.125	7.575	22.7	40.6
33	49.75	11.5	14.75	24.0
34	54.974999999999994	10.0	18.375	16.650000000000002
35	60.224999999999994	9.175	18.75	11.85
36	42.275	10.45	33.825	13.450000000000001
37	41.65	14.124999999999998	20.974999999999998	23.25
38	24.175	19.650000000000002	13.5	42.675000000000004
39	18.224999999999998	23.3	13.4	45.074999999999996
40	16.725	44.375	14.7	24.2
41	24.025	44.625	10.35	21.0
42	43.9	28.475	14.6	13.025
43	46.550000000000004	28.175	11.475	13.8
44	32.300000000000004	42.475	9.475	15.75
45	21.025	44.65	15.325	19.0
46	17.95	24.65	18.125	39.275
47	12.425	16.400000000000002	36.925000000000004	34.25
48	13.775	13.225000000000001	21.075	51.925
49	14.274999999999999	19.475	17.525	48.725
50	17.9	35.6	15.325	31.175000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.5
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	0.5
22	0.0
23	1.0
24	2.0
25	1.0
26	0.0
27	1.5
28	3.0
29	2.5
30	2.0
31	7.5
32	13.0
33	17.0
34	21.0
35	22.5
36	24.0
37	29.0
38	34.0
39	35.5
40	37.0
41	51.0
42	65.0
43	82.0
44	99.0
45	137.5
46	176.0
47	279.0
48	382.0
49	491.0
50	600.0
51	703.5
52	807.0
53	629.5
54	452.0
55	492.5
56	533.0
57	395.0
58	257.0
59	163.5
60	70.0
61	63.5
62	57.0
63	107.5
64	158.0
65	100.0
66	42.0
67	26.5
68	11.0
69	25.0
70	39.0
71	69.5
72	100.0
73	53.5
74	7.0
75	4.0
76	1.0
77	0.5
78	0.0
79	0.5
80	1.0
81	1.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82428115015975	29.275000000000002
2	10.351437699680512	8.1
3	4.025559105431309	4.725
4	2.4920127795527156	3.9
5	1.9808306709265175	3.875
6	0.8945686900958467	2.1
7	0.6389776357827476	1.7500000000000002
8	0.9584664536741214	3.0
9	0.6389776357827476	2.25
>10	2.619808306709265	19.35
>50	0.25559105431309903	7.3
>100	0.3194888178913738	14.374999999999998
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	129	3.225	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	128	3.2	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	114	2.85	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	82	2.0500000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	82	2.0500000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	71	1.775	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	57	1.425	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	47	1.175	RNA PCR Primer, Index 7 (97% over 35bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	44	1.0999999999999999	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	32	0.8	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	30	0.75	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	25	0.625	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	17	0.42500000000000004	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC	16	0.4	RNA PCR Primer, Index 42 (100% over 50bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	15	0.375	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC	12	0.3	RNA PCR Primer, Index 42 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	12	0.3	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	11	0.27499999999999997	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	10	0.25	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	9	0.22499999999999998	No Hit
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	8	0.2	RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGCATTCTCGGGTGCC	8	0.2	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC	8	0.2	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA	8	0.2	RNA PCR Primer, Index 3 (97% over 36bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTAT	6	0.15	RNA PCR Primer, Index 42 (100% over 48bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGAATTGTGGCTGGACATCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	6	0.15	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	6	0.15	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	6	0.15	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	5	0.125	No Hit
AGAAATGCGGATATGGGACTGAGATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATGGAAT	5	0.125	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.425	0.0	0.0	0.0
2	0.0	0.425	0.0	0.0	0.0
3	0.0	0.625	0.0	0.0	0.0
4	0.0	0.725	0.0	0.0	0.0
5	0.0	0.8	0.0	0.0	0.0
6	0.0	0.875	0.0	0.0	0.0
7	0.0	1.275	0.0	0.0	0.0
8	0.0	1.525	0.0	0.0	0.0
9	0.0	1.55	0.0	0.0	0.0
10	0.0	1.6	0.0	0.0	0.0
11	0.0	1.75	0.0	0.0	0.0
12	0.0	2.05	0.0	0.0	0.0
13	0.0	2.4	0.0	0.0	0.0
14	0.0	2.7	0.0	0.0	0.0
15	0.0	3.275	0.0	0.0	0.0
16	0.0	5.3	0.0	0.0	0.0
17	0.0	6.15	0.0	0.0	0.0
18	0.0	7.625	0.0	0.0	0.0
19	0.0	8.45	0.0	0.0	0.0
20	0.0	9.5	0.0	0.0	0.0
21	0.0	14.425	0.0	0.0	0.0
22	0.0	24.65	0.0	0.0	0.0
23	0.0	49.35	0.0	0.0	0.0
24	0.0	60.6	0.0	0.0	0.0
25	0.0	66.2	0.0	0.0	0.0
26	0.0	67.7	0.0	0.0	0.0
27	0.0	68.65	0.0	0.0	0.0
28	0.0	69.35	0.0	0.0	0.0
29	0.0	69.95	0.0	0.0	0.0
30	0.0	70.475	0.0	0.0	0.0
31	0.0	71.3	0.0	0.0	0.0
32	0.0	72.75	0.0	0.0	0.0
33	0.0	73.8	0.0	0.0	0.0
34	0.0	78.4	0.0	0.0	0.0
35	0.0	79.975	0.0	0.0	0.0
36	0.0	80.825	0.0	0.0	0.0
37	0.0	82.425	0.0	0.0	0.0
38	0.0	83.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
CTGAGCT	45	2.728484E-10	44.000004	11
CTGAGAC	20	6.952051E-4	44.000004	4
CTTTAAC	20	6.952051E-4	44.000004	12
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
CCTGAGA	20	6.952051E-4	44.000004	3
TCGGATC	45	2.728484E-10	44.000004	1
AACCTGT	20	6.952051E-4	44.000004	16
CGGATCC	45	2.728484E-10	44.000004	2
ACATTCA	40	5.315087E-9	44.000004	2
TAACCTG	20	6.952051E-4	44.000004	15
GCTGTCG	40	5.315087E-9	44.000004	11
GATCCGT	45	2.728484E-10	44.000004	4
ACCCTTT	20	6.952051E-4	44.000004	9
CCTTTAA	20	6.952051E-4	44.000004	11
CCCTGAG	20	6.952051E-4	44.000004	2
TTGGCTT	20	6.952051E-4	44.000004	17
TCCGTCT	45	2.728484E-10	44.000004	6
>>END_MODULE
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
Read 243552 spots for SRR5031022.sra
Written 243552 spots for SRR5031022.sra
SRR ids: ['SRR5031022.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xgws_sa4
SRR5031022.sra spots: 2922624
blocks: [[1, 243552], [243553, 487104], [487105, 730656], [730657, 974208], [974209, 1217760], [1217761, 1461312], [1461313, 1704864], [1704865, 1948416], [1948417, 2191968], [2191969, 2435520], [2435521, 2679072], [2679073, 2922624]]
SRR5031022 file size 505487
SRR5031022 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031022 SRR5031022_1.fastq
Input file:	SRR5031022_1.fastq
trimmed:	SRR5031022-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:08:26 2020 >> started

Wed Mar  4 07:08:28 2020 >> done (1.847s)
2922624 reads processed; of these:
    296 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
2922324 (99.99%) reads available; of these:
 182813 ( 6.26%) trimmed reads available after processing
2739511 (93.74%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     72	  0.00%
 19	     96	  0.00%
 20	    180	  0.01%
 21	    207	  0.01%
 22	    378	  0.01%
 23	    447	  0.02%
 24	    479	  0.02%
 25	    634	  0.02%
 26	    553	  0.02%
 27	    721	  0.02%
 28	    828	  0.03%
 29	   1252	  0.04%
 30	    826	  0.03%
 31	    942	  0.03%
 32	   1194	  0.04%
 33	   1427	  0.05%
 34	   1528	  0.05%
 35	   2981	  0.10%
 36	   2662	  0.09%
 37	   3081	  0.11%
 38	   3097	  0.11%
 39	   3371	  0.12%
 40	   3692	  0.13%
 41	   3794	  0.13%
 42	   5099	  0.17%
 43	   7384	  0.25%
 44	  10025	  0.34%
 45	  13033	  0.45%
 46	  19716	  0.67%
 47	  22210	  0.76%
 48	  30248	  1.04%
 49	  40656	  1.39%
 50	2739511	 93.74%
2922324 reads passed initial QC


criterion=sequence-density
sequence-density=82.32
sequence-density-rank=1
fanout-score=20.96
fanout-score-rank=1
prefix-density=84.95
prefix-fanout=20.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC


criterion=fanout-score
sequence-density=82.32
sequence-density-rank=1
fanout-score=20.96
fanout-score-rank=1
prefix-density=84.95
prefix-fanout=20.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC -o SRR5031022 -
Input file:	STDIN
trimmed:	SRR5031022-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:08:43 2020 >> started

Wed Mar  4 07:08:47 2020 >> done (3.780s)
2851907 reads processed; of these:
 211142 ( 7.40%) short reads filtered out after trimming by size control
  15602 ( 0.55%) empty reads filtered out after trimming by size control
2625163 (92.05%) reads available; of these:
2446198 (93.18%) trimmed reads available after processing
 178965 ( 6.82%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  16324	  0.62%
 19	  29840	  1.14%
 20	 144384	  5.50%
 21	 270820	 10.32%
 22	 703539	 26.80%
 23	 323221	 12.31%
 24	 161129	  6.14%
 25	  47080	  1.79%
 26	  25502	  0.97%
 27	  21126	  0.80%
 28	  22156	  0.84%
 29	  17983	  0.69%
 30	  26155	  1.00%
 31	  39344	  1.50%
 32	  37676	  1.44%
 33	 151041	  5.75%
 34	  44925	  1.71%
 35	  36394	  1.39%
 36	  42005	  1.60%
 37	  21347	  0.81%
 38	  23921	  0.91%
 39	  20424	  0.78%
 40	  30386	  1.16%
 41	  35594	  1.36%
 42	  40629	  1.55%
 43	  30280	  1.15%
 44	  23995	  0.91%
 45	  34490	  1.31%
 46	  25533	  0.97%
 47	  19688	  0.75%
 48	   1759	  0.07%
 49	   3247	  0.12%
 50	 153226	  5.84%


criterion=sequence-density
sequence-density=7.19
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.70
sequence-density-rank=20
fanout-score=11.88
fanout-score-rank=1
prefix-density=8.28
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 07:09:23
                             Started mapping on |	Mar 04 07:09:23
                                    Finished on |	Mar 04 07:09:48
       Mapping speed, Million of reads per hour |	388.16

                          Number of input reads |	2695580
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1375410
                        Uniquely mapped reads % |	51.02%
                          Average mapped length |	25.32
                       Number of splices: Total |	1743
            Number of splices: Annotated (sjdb) |	283
                       Number of splices: GT/AG |	1635
                       Number of splices: GC/AG |	20
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	88
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.80
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	815719
             % of reads mapped to multiple loci |	30.26%
        Number of reads mapped to too many loci |	87983
             % of reads mapped to too many loci |	3.26%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.21%
                     % of reads unmapped: other |	1.24%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	504451	504451	504451
N_multimapping	815719	815719	815719
N_noFeature	75635	96592	905046
N_ambiguous	754028	338351	14989
UnstrandedReadsAssigned:545747 PositiveStrandReadsAssigned:940467 NegativeStrandReadsAssigned:455375
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031022 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031022-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,695,580 reads, 660,684 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,152 rounds

 109283 SRR5031022.ke.tsv
  52636 SRR5031022.se.tsv
 161919 total
==> SRR5031022.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031022.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031022 completed mapping pipeline successfully
