Starting /dee2/code/volunteer_pipeline.sh SRR5030834
    current disk space = 160627064832
    free memory = 73934940 
SRR5030834 SRAfilesize
85eaa645bf7870e8879105b025fdb944  SRR5030834.sra
SRR5030834.sra file validated
SRR5030834 is single end
SRR5030834 is conventional basespace
SRR5030834 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38975	33.0	31.0	34.0	31.0	34.0
2	32.71075	34.0	31.0	34.0	31.0	34.0
3	32.736	34.0	31.0	34.0	31.0	34.0
4	36.2305	37.0	37.0	37.0	35.0	37.0
5	36.2905	37.0	37.0	37.0	35.0	37.0
6	36.07575	37.0	35.0	37.0	35.0	37.0
7	36.0485	37.0	35.0	37.0	35.0	37.0
8	36.128	37.0	36.0	37.0	35.0	37.0
9	37.9095	39.0	38.0	39.0	35.0	39.0
10	37.79575	39.0	38.0	39.0	35.0	39.0
11	37.7615	39.0	38.0	39.0	35.0	39.0
12	37.73525	39.0	38.0	39.0	35.0	39.0
13	37.67425	39.0	37.0	39.0	35.0	39.0
14	39.08775	40.0	38.0	41.0	35.0	41.0
15	39.12775	40.0	38.0	41.0	36.0	41.0
16	39.04075	40.0	38.0	41.0	35.0	41.0
17	39.153	40.0	38.0	41.0	36.0	41.0
18	39.22775	41.0	39.0	41.0	36.0	41.0
19	39.258	41.0	39.0	41.0	36.0	41.0
20	39.102	41.0	39.0	41.0	35.0	41.0
21	39.10475	40.0	39.0	41.0	35.0	41.0
22	39.23525	41.0	39.0	41.0	36.0	41.0
23	39.23025	41.0	39.0	41.0	36.0	41.0
24	38.50675	40.0	38.0	41.0	34.0	41.0
25	38.80825	40.0	39.0	41.0	35.0	41.0
26	38.94925	40.0	38.0	41.0	35.0	41.0
27	38.96175	40.0	39.0	41.0	35.0	41.0
28	38.59375	40.0	38.0	41.0	34.0	41.0
29	38.12025	40.0	38.0	41.0	33.0	41.0
30	38.2615	40.0	38.0	41.0	33.0	41.0
31	38.698	40.0	38.0	41.0	35.0	41.0
32	38.772	40.0	38.0	41.0	34.0	41.0
33	37.758	40.0	38.0	41.0	32.0	41.0
34	37.633	40.0	37.0	41.0	32.0	41.0
35	37.115	39.0	37.0	40.0	31.0	41.0
36	33.0615	38.0	30.0	40.0	16.0	41.0
37	35.1	38.0	33.0	40.0	26.0	41.0
38	36.68525	38.0	35.0	40.0	31.0	41.0
39	36.55275	39.0	35.0	40.0	30.0	41.0
40	36.8735	39.0	36.0	40.0	31.0	41.0
41	36.515	39.0	35.0	40.0	30.0	41.0
42	36.437	39.0	36.0	40.0	30.0	41.0
43	36.581	39.0	36.0	40.0	30.0	41.0
44	36.981	39.0	37.0	41.0	32.0	41.0
45	36.51225	39.0	36.0	40.0	30.0	41.0
46	34.0865	38.0	33.0	40.0	23.0	41.0
47	35.4365	38.0	34.0	40.0	28.0	41.0
48	35.5415	39.0	35.0	40.0	29.0	41.0
49	34.72375	38.0	34.0	40.0	27.0	41.0
50	30.42525	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	0.0
16	1.0
17	1.0
18	4.0
19	4.0
20	4.0
21	6.0
22	6.0
23	11.0
24	8.0
25	20.0
26	22.0
27	28.0
28	38.0
29	33.0
30	46.0
31	74.0
32	100.0
33	123.0
34	169.0
35	229.0
36	326.0
37	583.0
38	1050.0
39	1110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.974693059383613	24.85592583312453	47.33149586569782	16.837885241794037
2	22.675	24.175	21.099999999999998	32.05
3	24.825	21.175	21.05	32.95
4	29.725	27.275	15.475	27.525
5	21.25	43.75	22.275	12.725
6	34.275	17.349999999999998	34.5	13.875000000000002
7	19.650000000000002	18.9	25.724999999999998	35.725
8	25.650000000000002	29.7	14.625	30.025000000000002
9	22.225	33.475	29.625	14.674999999999999
10	23.674999999999997	21.5	30.85	23.974999999999998
11	30.15	14.224999999999998	16.225	39.4
12	15.525	21.925	29.625	32.925
13	30.75	20.325	28.050000000000004	20.875
14	27.750000000000004	32.05	21.275	18.925
15	32.025	24.425	27.275	16.275000000000002
16	18.45	23.5	21.25	36.8
17	23.0	21.5	40.9	14.6
18	22.725	20.025000000000002	31.7	25.55
19	36.4	16.150000000000002	28.225	19.225
20	39.7	17.45	28.175	14.674999999999999
21	23.875	18.75	28.299999999999997	29.075
22	27.175	14.875	44.75	13.200000000000001
23	26.700000000000003	14.374999999999998	41.949999999999996	16.975
24	49.575	15.9	23.799999999999997	10.725
25	48.025	23.65	15.125	13.200000000000001
26	26.924999999999997	44.875	14.099999999999998	14.099999999999998
27	18.925	44.800000000000004	22.900000000000002	13.375
28	14.224999999999998	24.45	45.375	15.950000000000001
29	11.200000000000001	15.049999999999999	54.074999999999996	19.675
30	10.674999999999999	9.775	33.800000000000004	45.75
31	16.175	7.3999999999999995	45.4	31.025000000000002
32	26.900000000000002	9.0	21.675	42.425000000000004
33	51.324999999999996	11.600000000000001	14.95	22.125
34	55.800000000000004	10.475	17.7	16.025
35	59.050000000000004	8.975	18.325	13.65
36	42.35	10.875	32.5	14.274999999999999
37	43.525000000000006	11.275	21.224999999999998	23.974999999999998
38	23.1	16.575	15.6	44.725
39	18.55	23.599999999999998	11.65	46.2
40	16.950000000000003	44.925	15.6	22.525000000000002
41	25.75	45.25	11.675	17.325
42	48.125	27.85	13.825000000000001	10.2
43	47.3	30.575000000000003	11.35	10.775
44	30.525000000000002	46.949999999999996	9.6	12.925
45	18.85	49.025	14.249999999999998	17.875
46	14.325	28.000000000000004	19.8	37.875
47	10.5	17.275	39.025	33.2
48	14.025000000000002	13.725000000000001	19.625	52.625
49	15.35	21.525	15.675	47.449999999999996
50	20.175	35.199999999999996	14.899999999999999	29.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	2.0
22	3.0
23	2.0
24	1.0
25	1.0
26	1.0
27	2.0
28	3.0
29	3.0
30	3.0
31	16.0
32	29.0
33	31.0
34	33.0
35	42.5
36	52.0
37	64.5
38	77.0
39	66.5
40	56.0
41	55.0
42	54.0
43	96.5
44	139.0
45	181.5
46	224.0
47	308.5
48	393.0
49	508.5
50	624.0
51	707.5
52	791.0
53	610.5
54	430.0
55	446.0
56	462.0
57	354.5
58	247.0
59	155.0
60	63.0
61	57.5
62	52.0
63	90.5
64	129.0
65	89.0
66	49.0
67	32.0
68	15.0
69	16.0
70	17.0
71	23.5
72	30.0
73	17.5
74	5.0
75	4.5
76	4.0
77	3.0
78	2.0
79	2.5
80	3.0
81	2.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22499999999999998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05633802816901	31.05
2	9.614206981016533	7.85
3	4.164115125535823	5.1
4	1.7146356399265157	2.8000000000000003
5	1.8371096142069812	3.75
6	0.9185548071034906	2.25
7	0.6736068585425597	1.925
8	0.4286589099816289	1.4000000000000001
9	0.4898958971218616	1.7999999999999998
>10	3.551745254133497	24.45
>50	0.4286589099816289	11.825
>100	0.1224739742804654	5.800000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	114	2.85	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	85	2.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	84	2.1	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	75	1.875	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	65	1.625	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	55	1.375	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	46	1.15	No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	29	0.7250000000000001	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	25	0.625	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	25	0.625	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	19	0.475	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG	17	0.42500000000000004	RNA PCR Primer, Index 2 (97% over 35bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTC	17	0.42500000000000004	RNA PCR Primer, Index 14 (100% over 44bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	16	0.4	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	16	0.4	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	14	0.35000000000000003	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 14 (100% over 50bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	14	0.35000000000000003	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	13	0.325	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	13	0.325	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	13	0.325	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	12	0.3	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	11	0.27499999999999997	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	11	0.27499999999999997	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	11	0.27499999999999997	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	10	0.25	RNA PCR Primer, Index 1 (100% over 33bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	10	0.25	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	9	0.22499999999999998	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	8	0.2	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
TGCGGGGCTAGGGCTAACAGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
CTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAG	6	0.15	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	6	0.15	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATTTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGATGATGAGCCCATTGCCCTGAGCTCTTGTGATGCTGGCTTCAAAG	5	0.125	No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	5	0.125	No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGTG	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATGGAAT	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	5	0.125	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.35	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.475	0.0	0.0	0.0
4	0.0	0.5	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.6	0.0	0.0	0.0
7	0.0	1.2	0.0	0.0	0.0
8	0.0	1.325	0.0	0.0	0.0
9	0.0	1.35	0.0	0.0	0.0
10	0.0	1.475	0.0	0.0	0.0
11	0.0	1.575	0.0	0.0	0.0
12	0.0	1.675	0.0	0.0	0.0
13	0.0	1.825	0.0	0.0	0.0
14	0.0	1.975	0.0	0.0	0.0
15	0.0	2.35	0.0	0.0	0.0
16	0.0	3.1	0.0	0.0	0.0
17	0.0	3.75	0.0	0.0	0.0
18	0.0	4.5	0.0	0.0	0.0
19	0.0	4.825	0.0	0.0	0.0
20	0.0	5.575	0.0	0.0	0.0
21	0.0	10.35	0.0	0.0	0.0
22	0.0	20.425	0.0	0.0	0.0
23	0.0	47.0	0.0	0.0	0.0
24	0.0	57.45	0.0	0.0	0.0
25	0.0	63.2	0.0	0.0	0.0
26	0.0	64.725	0.0	0.0	0.0
27	0.0	65.475	0.0	0.0	0.0
28	0.0	65.925	0.0	0.0	0.0
29	0.0	66.425	0.0	0.0	0.0
30	0.0	66.8	0.0	0.0	0.0
31	0.0	67.425	0.0	0.0	0.0
32	0.0	68.95	0.0	0.0	0.0
33	0.0	69.7	0.0	0.0	0.0
34	0.0	72.575	0.0	0.0	0.0
35	0.0	73.5	0.0	0.0	0.0
36	0.0	74.475	0.0	0.0	0.0
37	0.0	75.375	0.0	0.0	0.0
38	0.0	76.05	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCAG	20	6.952051E-4	44.000004	22
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
CAGTCTG	20	6.952051E-4	44.000004	26
AAGTAAT	45	2.728484E-10	44.000004	4
TCAAGTA	45	2.728484E-10	44.000004	2
CCCAGTC	20	6.952051E-4	44.000004	24
AACCGGT	20	6.952051E-4	44.000004	9
ATCCAGG	40	5.315087E-9	44.000004	9
ACTGAAT	20	6.952051E-4	44.000004	12
CGCCCCA	20	6.952051E-4	44.000004	21
CCAGTCT	20	6.952051E-4	44.000004	25
TTCAAGT	45	2.728484E-10	44.000004	1
AACTGAA	20	6.952051E-4	44.000004	11
AGGATAG	40	5.315087E-9	44.000004	13
GGTCTCT	20	6.952051E-4	44.000004	13
CTCTTTT	20	6.952051E-4	44.000004	16
CCGCCCC	20	6.952051E-4	44.000004	20
TAGCTGT	20	6.952051E-4	44.000004	34
CCTGATG	20	6.952051E-4	44.000004	4
>>END_MODULE
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220116 spots for SRR5030834.sra
Written 220116 spots for SRR5030834.sra
Read 220118 spots for SRR5030834.sra
Written 220118 spots for SRR5030834.sra
SRR ids: ['SRR5030834.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gtvsvorl
SRR5030834.sra spots: 2641394
blocks: [[1, 220116], [220117, 440232], [440233, 660348], [660349, 880464], [880465, 1100580], [1100581, 1320696], [1320697, 1540812], [1540813, 1760928], [1760929, 1981044], [1981045, 2201160], [2201161, 2421276], [2421277, 2641394]]
SRR5030834 file size 456732
SRR5030834 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030834 SRR5030834_1.fastq
Input file:	SRR5030834_1.fastq
trimmed:	SRR5030834-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:23:48 2020 >> started

Wed Mar  4 06:23:51 2020 >> done (3.151s)
2641394 reads processed; of these:
    161 ( 0.01%) short reads filtered out after trimming by size control
     14 ( 0.00%) empty reads filtered out after trimming by size control
2641219 (99.99%) reads available; of these:
 202331 ( 7.66%) trimmed reads available after processing
2438888 (92.34%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     48	  0.00%
 19	     41	  0.00%
 20	    169	  0.01%
 21	    149	  0.01%
 22	    245	  0.01%
 23	    301	  0.01%
 24	    360	  0.01%
 25	    418	  0.02%
 26	    393	  0.01%
 27	    497	  0.02%
 28	    570	  0.02%
 29	    841	  0.03%
 30	    670	  0.03%
 31	    918	  0.03%
 32	   1334	  0.05%
 33	   2241	  0.08%
 34	   8602	  0.33%
 35	   4089	  0.15%
 36	   2212	  0.08%
 37	   2525	  0.10%
 38	   3765	  0.14%
 39	   3521	  0.13%
 40	   5369	  0.20%
 41	   4249	  0.16%
 42	   6348	  0.24%
 43	   5242	  0.20%
 44	  15280	  0.58%
 45	  13680	  0.52%
 46	  12059	  0.46%
 47	  15377	  0.58%
 48	  36865	  1.40%
 49	  53953	  2.04%
 50	2438888	 92.34%
2641219 reads passed initial QC


criterion=sequence-density
sequence-density=75.75
sequence-density-rank=1
fanout-score=21.35
fanout-score-rank=2
prefix-density=78.33
prefix-fanout=20.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC


criterion=fanout-score
sequence-density=2.01
sequence-density-rank=6
fanout-score=117.46
fanout-score-rank=1
prefix-density=77.66
prefix-fanout=3.0
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC -o SRR5030834 -
Input file:	STDIN
trimmed:	SRR5030834-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:24:13 2020 >> started

Wed Mar  4 06:24:18 2020 >> done (5.130s)
2571713 reads processed; of these:
 111068 ( 4.32%) short reads filtered out after trimming by size control
   9012 ( 0.35%) empty reads filtered out after trimming by size control
2451633 (95.33%) reads available; of these:
2109839 (86.06%) trimmed reads available after processing
 341794 (13.94%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13785	  0.56%
 19	  20084	  0.82%
 20	 123320	  5.03%
 21	 277579	 11.32%
 22	 679456	 27.71%
 23	 289536	 11.81%
 24	 143964	  5.87%
 25	  41365	  1.69%
 26	  20018	  0.82%
 27	  14621	  0.60%
 28	  15602	  0.64%
 29	  11706	  0.48%
 30	  19850	  0.81%
 31	  35660	  1.45%
 32	  23946	  0.98%
 33	  66610	  2.72%
 34	  24491	  1.00%
 35	  20605	  0.84%
 36	  23348	  0.95%
 37	  14082	  0.57%
 38	  15156	  0.62%
 39	  15624	  0.64%
 40	  24546	  1.00%
 41	  34442	  1.40%
 42	  41242	  1.68%
 43	  28109	  1.15%
 44	  23975	  0.98%
 45	  32879	  1.34%
 46	  22669	  0.92%
 47	  17893	  0.73%
 48	   4240	  0.17%
 49	  12889	  0.53%
 50	 298341	 12.17%


criterion=sequence-density
sequence-density=4.06
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AAGCTGCCAGTTGAAGAACTGTTGGAATTCTC


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=37
fanout-score=34.29
fanout-score-rank=1
prefix-density=0.99
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:24:50
                             Started mapping on |	Mar 04 06:24:51
                                    Finished on |	Mar 04 06:25:14
       Mapping speed, Million of reads per hour |	394.61

                          Number of input reads |	2521139
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1490048
                        Uniquely mapped reads % |	59.10%
                          Average mapped length |	28.56
                       Number of splices: Total |	951
            Number of splices: Annotated (sjdb) |	184
                       Number of splices: GT/AG |	858
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	75
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.39
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	714888
             % of reads mapped to multiple loci |	28.36%
        Number of reads mapped to too many loci |	55439
             % of reads mapped to too many loci |	2.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.78%
                     % of reads unmapped: other |	0.56%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	316203	316203	316203
N_multimapping	714888	714888	714888
N_noFeature	83674	103677	1075837
N_ambiguous	805678	456697	17220
UnstrandedReadsAssigned:600696 PositiveStrandReadsAssigned:929674 NegativeStrandReadsAssigned:396991
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030834 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030834-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,521,139 reads, 651,729 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,131 rounds

 109283 SRR5030834.ke.tsv
  52636 SRR5030834.se.tsv
 161919 total
==> SRR5030834.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030834.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030834 completed mapping pipeline successfully
