Starting /dee2/code/volunteer_pipeline.sh SRR5030961
    current disk space = 160579502080
    free memory = 73949480 
SRR5030961 SRAfilesize
eff6ec82445b5cbd59b48049cec7a284  SRR5030961.sra
SRR5030961.sra file validated
SRR5030961 is single end
SRR5030961 is conventional basespace
SRR5030961 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37725	33.0	31.0	34.0	31.0	34.0
2	32.6785	34.0	31.0	34.0	31.0	34.0
3	32.8165	34.0	31.0	34.0	31.0	34.0
4	36.2155	37.0	37.0	37.0	35.0	37.0
5	36.22275	37.0	37.0	37.0	35.0	37.0
6	36.11175	37.0	36.0	37.0	35.0	37.0
7	36.07075	37.0	35.0	37.0	35.0	37.0
8	36.1825	37.0	36.0	37.0	35.0	37.0
9	37.97125	39.0	38.0	39.0	35.0	39.0
10	38.02125	39.0	38.0	39.0	35.0	39.0
11	37.87525	39.0	38.0	39.0	35.0	39.0
12	37.881	39.0	38.0	39.0	35.0	39.0
13	37.79825	39.0	38.0	39.0	35.0	39.0
14	39.1595	41.0	39.0	41.0	36.0	41.0
15	39.157	41.0	39.0	41.0	36.0	41.0
16	39.24025	41.0	39.0	41.0	36.0	41.0
17	39.35825	41.0	39.0	41.0	36.0	41.0
18	39.32575	41.0	39.0	41.0	36.0	41.0
19	38.9435	40.0	39.0	41.0	35.0	41.0
20	39.05525	40.0	39.0	41.0	35.0	41.0
21	39.13225	40.0	39.0	41.0	36.0	41.0
22	39.0215	40.0	39.0	41.0	36.0	41.0
23	38.89375	40.0	39.0	41.0	35.0	41.0
24	38.60575	40.0	38.0	41.0	34.0	41.0
25	38.95225	40.0	39.0	41.0	35.0	41.0
26	38.8695	40.0	38.0	41.0	35.0	41.0
27	38.933	40.0	39.0	41.0	35.0	41.0
28	38.754	40.0	38.0	41.0	34.0	41.0
29	38.02025	40.0	38.0	41.0	33.0	41.0
30	38.19175	40.0	38.0	41.0	33.0	41.0
31	38.50025	40.0	38.0	41.0	34.0	41.0
32	38.6915	40.0	38.0	41.0	35.0	41.0
33	37.9035	40.0	38.0	41.0	33.0	41.0
34	37.74825	40.0	38.0	41.0	33.0	41.0
35	37.57425	39.0	37.0	40.0	33.0	41.0
36	34.0795	38.0	31.0	40.0	23.0	41.0
37	35.2555	38.0	34.0	40.0	27.0	41.0
38	36.7815	39.0	36.0	40.0	31.0	41.0
39	36.80175	39.0	36.0	40.0	31.0	41.0
40	36.669	39.0	36.0	40.0	31.0	41.0
41	36.68375	39.0	36.0	40.0	30.0	41.0
42	36.88525	40.0	37.0	41.0	30.0	41.0
43	36.75775	39.0	36.0	41.0	31.0	41.0
44	36.714	39.0	36.0	41.0	31.0	41.0
45	36.35975	39.0	36.0	40.0	30.0	41.0
46	34.676	38.0	34.0	40.0	24.0	41.0
47	35.265	38.0	35.0	40.0	27.0	41.0
48	35.548	39.0	35.0	40.0	29.0	41.0
49	34.47425	38.0	34.0	40.0	26.0	41.0
50	29.51075	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	0.0
16	1.0
17	2.0
18	2.0
19	6.0
20	6.0
21	5.0
22	12.0
23	11.0
24	17.0
25	14.0
26	21.0
27	17.0
28	28.0
29	54.0
30	51.0
31	68.0
32	89.0
33	130.0
34	162.0
35	197.0
36	301.0
37	581.0
38	1044.0
39	1178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.27254509018036	24.39879759519038	49.79959919839679	14.529058116232466
2	22.55	27.35	21.05	29.049999999999997
3	25.324999999999996	17.2	23.3	34.175
4	32.15	29.275000000000002	14.149999999999999	24.425
5	19.8	39.45	25.6	15.15
6	29.275000000000002	18.625	39.2	12.9
7	20.9	19.825	22.0	37.275000000000006
8	22.0	32.25	15.375	30.375000000000004
9	24.525	33.475	26.375	15.625
10	25.95	15.825	31.624999999999996	26.6
11	29.7	15.275	16.7	38.324999999999996
12	16.625	21.0	32.35	30.025000000000002
13	28.025	19.925	28.849999999999998	23.200000000000003
14	27.625	32.0	22.95	17.424999999999997
15	35.675000000000004	22.3	26.224999999999998	15.8
16	16.5	21.875	24.075	37.55
17	24.875	21.525	37.15	16.45
18	25.374999999999996	18.475	32.7	23.45
19	35.35	15.2	31.324999999999996	18.125
20	41.199999999999996	18.075	24.975	15.75
21	23.575	21.525	24.5	30.4
22	29.099999999999998	13.825000000000001	45.35	11.725
23	27.125	14.45	42.175000000000004	16.25
24	48.55	16.225	24.725	10.5
25	47.9	24.175	15.975	11.95
26	27.224999999999998	44.275	15.425	13.075000000000001
27	17.275	44.6	25.0	13.125
28	14.149999999999999	23.65	46.150000000000006	16.05
29	11.924999999999999	14.924999999999999	54.574999999999996	18.575
30	11.799999999999999	8.575000000000001	33.425	46.2
31	17.925	7.025	43.2	31.85
32	27.55	8.774999999999999	20.7	42.975
33	52.775000000000006	9.85	14.224999999999998	23.150000000000002
34	56.699999999999996	9.875	18.4	15.024999999999999
35	60.699999999999996	8.225	18.825	12.25
36	41.5	10.775	33.175	14.549999999999999
37	43.475	11.700000000000001	19.3	25.525
38	23.0	18.275	14.224999999999998	44.5
39	18.075	25.275	9.5	47.15
40	18.175	45.925	12.775	23.125
41	25.25	45.550000000000004	12.15	17.05
42	48.55	29.25	10.975	11.225
43	47.449999999999996	31.624999999999996	10.549999999999999	10.375
44	28.775000000000002	46.625	8.799999999999999	15.8
45	17.825	48.55	15.075	18.55
46	12.55	27.450000000000003	18.8	41.199999999999996
47	10.5	17.525	37.5	34.475
48	12.1	15.25	19.775000000000002	52.87500000000001
49	16.625	20.5	15.1	47.775
50	19.6	36.6	15.75	28.050000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	1.0
24	2.0
25	1.5
26	1.0
27	2.0
28	3.0
29	2.5
30	2.0
31	11.0
32	20.0
33	24.0
34	28.0
35	42.0
36	56.0
37	64.5
38	73.0
39	67.5
40	62.0
41	69.5
42	77.0
43	97.5
44	118.0
45	156.5
46	195.0
47	313.0
48	431.0
49	521.0
50	611.0
51	686.5
52	762.0
53	606.5
54	451.0
55	467.5
56	484.0
57	378.0
58	272.0
59	176.0
60	80.0
61	68.0
62	56.0
63	64.0
64	72.0
65	50.0
66	28.0
67	19.0
68	10.0
69	18.0
70	26.0
71	44.5
72	63.0
73	35.0
74	7.0
75	5.0
76	3.0
77	2.5
78	2.0
79	1.5
80	1.0
81	1.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.04895104895105	32.625
2	9.73193473193473	8.35
3	3.9627039627039626	5.1
4	2.4475524475524475	4.2
5	1.5151515151515151	3.25
6	1.048951048951049	2.7
7	0.5827505827505828	1.7500000000000002
8	0.641025641025641	2.1999999999999997
9	0.5827505827505828	2.25
>10	2.9137529137529135	19.5
>50	0.4662004662004662	15.4
>100	0.05827505827505827	2.675
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	107	2.675	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	100	2.5	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	96	2.4	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	93	2.325	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	76	1.9	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	71	1.775	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	59	1.4749999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 35bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	25	0.625	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	25	0.625	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC	23	0.575	RNA PCR Primer, Index 26 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	16	0.4	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	16	0.4	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	15	0.375	RNA PCR Primer, Index 1 (100% over 33bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	15	0.375	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC	13	0.325	RNA PCR Primer, Index 26 (100% over 50bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	13	0.325	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	12	0.3	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	10	0.25	No Hit
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	9	0.22499999999999998	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	9	0.22499999999999998	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	8	0.2	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	8	0.2	RNA PCR Primer, Index 1 (100% over 34bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTAT	8	0.2	RNA PCR Primer, Index 26 (100% over 48bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	8	0.2	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	8	0.2	No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	8	0.2	RNA PCR Primer, Index 1 (100% over 35bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	8	0.2	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	7	0.17500000000000002	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	7	0.17500000000000002	RNA PCR Primer, Index 1 (97% over 37bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	6	0.15	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	6	0.15	No Hit
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	5	0.125	RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	5	0.125	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	5	0.125	No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	5	0.125	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGACACTCTGTGCTGAATGTC	5	0.125	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.6	0.0	0.0	0.0
4	0.0	0.6	0.0	0.0	0.0
5	0.0	0.675	0.0	0.0	0.0
6	0.0	0.75	0.0	0.0	0.0
7	0.0	1.475	0.0	0.0	0.0
8	0.0	1.55	0.0	0.0	0.0
9	0.0	1.775	0.0	0.0	0.0
10	0.0	1.875	0.0	0.0	0.0
11	0.0	2.275	0.0	0.0	0.0
12	0.0	2.675	0.0	0.0	0.0
13	0.0	2.825	0.0	0.0	0.0
14	0.0	3.3	0.0	0.0	0.0
15	0.0	3.75	0.0	0.0	0.0
16	0.0	5.25	0.0	0.0	0.0
17	0.0	6.175	0.0	0.0	0.0
18	0.0	6.925	0.0	0.0	0.0
19	0.0	7.65	0.0	0.0	0.0
20	0.0	8.5	0.0	0.0	0.0
21	0.0	14.425	0.0	0.0	0.0
22	0.0	24.45	0.0	0.0	0.0
23	0.0	51.55	0.0	0.0	0.0
24	0.0	62.2	0.0	0.0	0.0
25	0.0	67.625	0.0	0.0	0.0
26	0.0	68.95	0.0	0.0	0.0
27	0.0	69.45	0.0	0.0	0.0
28	0.0	70.15	0.0	0.0	0.0
29	0.0	70.825	0.0	0.0	0.0
30	0.0	71.25	0.0	0.0	0.0
31	0.0	71.95	0.0	0.0	0.0
32	0.0	72.975	0.0	0.0	0.0
33	0.0	73.725	0.0	0.0	0.0
34	0.0	75.65	0.0	0.0	0.0
35	0.0	76.8	0.0	0.0	0.0
36	0.0	77.4	0.0	0.0	0.0
37	0.0	78.25	0.0	0.0	0.0
38	0.0	78.75	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGGA	20	6.952051E-4	44.000004	20
TAAACAT	20	6.952051E-4	44.000004	3
TTGTATA	20	6.952051E-4	44.000004	13
AAACATC	20	6.952051E-4	44.000004	4
ATGATGA	20	6.952051E-4	44.000004	5
CGGGGGC	20	6.952051E-4	44.000004	32
GTATAGT	20	6.952051E-4	44.000004	15
CTTGGCT	20	6.952051E-4	44.000004	16
ACCCTTT	20	6.952051E-4	44.000004	9
CGGTGAG	20	6.952051E-4	44.000004	16
CCTTTAA	20	6.952051E-4	44.000004	11
GGCCAAG	20	6.952051E-4	44.000004	36
GCTTGGC	20	6.952051E-4	44.000004	15
CTCGGGG	20	6.952051E-4	44.000004	30
GGGGCCA	20	6.952051E-4	44.000004	34
GACCCTT	20	6.952051E-4	44.000004	8
CCCTTTA	20	6.952051E-4	44.000004	10
TGTATAG	20	6.952051E-4	44.000004	14
TATAGTT	20	6.952051E-4	44.000004	16
TGTAAAC	20	6.952051E-4	44.000004	1
>>END_MODULE
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
Read 204637 spots for SRR5030961.sra
Written 204637 spots for SRR5030961.sra
Read 204636 spots for SRR5030961.sra
Written 204636 spots for SRR5030961.sra
SRR ids: ['SRR5030961.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9dp2sgsm
SRR5030961.sra spots: 2455633
blocks: [[1, 204636], [204637, 409272], [409273, 613908], [613909, 818544], [818545, 1023180], [1023181, 1227816], [1227817, 1432452], [1432453, 1637088], [1637089, 1841724], [1841725, 2046360], [2046361, 2250996], [2250997, 2455633]]
SRR5030961 file size 424538
SRR5030961 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030961 SRR5030961_1.fastq
Input file:	SRR5030961_1.fastq
trimmed:	SRR5030961-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:54:48 2020 >> started

Wed Mar  4 06:54:51 2020 >> done (2.958s)
2455633 reads processed; of these:
    103 ( 0.00%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
2455525 (100.00%) reads available; of these:
 147843 ( 6.02%) trimmed reads available after processing
2307682 (93.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     47	  0.00%
 19	     54	  0.00%
 20	    121	  0.00%
 21	    146	  0.01%
 22	    247	  0.01%
 23	    274	  0.01%
 24	    309	  0.01%
 25	    397	  0.02%
 26	    347	  0.01%
 27	    467	  0.02%
 28	    611	  0.02%
 29	    877	  0.04%
 30	    604	  0.02%
 31	    797	  0.03%
 32	    790	  0.03%
 33	    993	  0.04%
 34	   2357	  0.10%
 35	   2033	  0.08%
 36	   1486	  0.06%
 37	   1701	  0.07%
 38	   2004	  0.08%
 39	   2122	  0.09%
 40	   2489	  0.10%
 41	   2729	  0.11%
 42	   3529	  0.14%
 43	   4688	  0.19%
 44	   8337	  0.34%
 45	  10192	  0.42%
 46	   9658	  0.39%
 47	  14257	  0.58%
 48	  28714	  1.17%
 49	  44466	  1.81%
 50	2307682	 93.98%
2455525 reads passed initial QC


criterion=sequence-density
sequence-density=79.38
sequence-density-rank=1
fanout-score=19.81
fanout-score-rank=1
prefix-density=81.25
prefix-fanout=19.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC


criterion=fanout-score
sequence-density=79.38
sequence-density-rank=1
fanout-score=19.81
fanout-score-rank=1
prefix-density=81.25
prefix-fanout=19.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC -o SRR5030961 -
Input file:	STDIN
trimmed:	SRR5030961-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:55:09 2020 >> started

Wed Mar  4 06:55:16 2020 >> done (7.082s)
2394137 reads processed; of these:
 168461 ( 7.04%) short reads filtered out after trimming by size control
  14034 ( 0.59%) empty reads filtered out after trimming by size control
2211642 (92.38%) reads available; of these:
1938116 (87.63%) trimmed reads available after processing
 273526 (12.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  14267	  0.65%
 19	  22576	  1.02%
 20	 140182	  6.34%
 21	 253698	 11.47%
 22	 644636	 29.15%
 23	 267168	 12.08%
 24	 133286	  6.03%
 25	  35121	  1.59%
 26	  18027	  0.82%
 27	  14267	  0.65%
 28	  15867	  0.72%
 29	  11858	  0.54%
 30	  16864	  0.76%
 31	  25715	  1.16%
 32	  22930	  1.04%
 33	  46292	  2.09%
 34	  24489	  1.11%
 35	  21142	  0.96%
 36	  22388	  1.01%
 37	  12422	  0.56%
 38	  13863	  0.63%
 39	  13257	  0.60%
 40	  19755	  0.89%
 41	  24795	  1.12%
 42	  28695	  1.30%
 43	  20743	  0.94%
 44	  15770	  0.71%
 45	  23641	  1.07%
 46	  18386	  0.83%
 47	  14009	  0.63%
 48	   2742	  0.12%
 49	   8873	  0.40%
 50	 243918	 11.03%


criterion=sequence-density
sequence-density=6.60
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.69
sequence-density-rank=18
fanout-score=12.14
fanout-score-rank=1
prefix-density=8.42
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:55:46
                             Started mapping on |	Mar 04 06:55:47
                                    Finished on |	Mar 04 06:56:10
       Mapping speed, Million of reads per hour |	355.78

                          Number of input reads |	2273030
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1333548
                        Uniquely mapped reads % |	58.67%
                          Average mapped length |	27.08
                       Number of splices: Total |	1240
            Number of splices: Annotated (sjdb) |	150
                       Number of splices: GT/AG |	1165
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	60
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.62
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	651345
             % of reads mapped to multiple loci |	28.66%
        Number of reads mapped to too many loci |	49465
             % of reads mapped to too many loci |	2.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.52%
                     % of reads unmapped: other |	0.98%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	288137	288137	288137
N_multimapping	651345	651345	651345
N_noFeature	56949	73562	901819
N_ambiguous	741136	360579	12839
UnstrandedReadsAssigned:535463 PositiveStrandReadsAssigned:899407 NegativeStrandReadsAssigned:418890
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030961 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030961-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,273,030 reads, 601,467 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,117 rounds

 109283 SRR5030961.ke.tsv
  52636 SRR5030961.se.tsv
 161919 total
==> SRR5030961.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030961.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030961 completed mapping pipeline successfully
