Starting /dee2/code/volunteer_pipeline.sh SRR5031053
    current disk space = 160518455296
    free memory = 73946764 
SRR5031053 SRAfilesize
15f03a7038cfdaff543c65bacf9dbbda  SRR5031053.sra
SRR5031053.sra file validated
SRR5031053 is single end
SRR5031053 is conventional basespace
SRR5031053 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.425	33.0	31.0	34.0	31.0	34.0
2	32.6725	34.0	31.0	34.0	31.0	34.0
3	32.6295	34.0	31.0	34.0	31.0	34.0
4	36.2535	37.0	37.0	37.0	35.0	37.0
5	36.155	37.0	35.0	37.0	35.0	37.0
6	35.85975	37.0	35.0	37.0	33.0	37.0
7	35.98675	37.0	35.0	37.0	35.0	37.0
8	35.87625	37.0	35.0	37.0	35.0	37.0
9	37.64075	39.0	37.0	39.0	35.0	39.0
10	37.85325	39.0	38.0	39.0	35.0	39.0
11	37.69575	39.0	37.0	39.0	35.0	39.0
12	37.7425	39.0	38.0	39.0	35.0	39.0
13	37.6685	39.0	37.0	39.0	35.0	39.0
14	39.16575	41.0	39.0	41.0	36.0	41.0
15	39.1535	40.0	38.0	41.0	36.0	41.0
16	39.0	40.0	38.0	41.0	36.0	41.0
17	38.932	40.0	38.0	41.0	35.0	41.0
18	39.17075	41.0	39.0	41.0	36.0	41.0
19	38.748	40.0	38.0	41.0	34.0	41.0
20	38.997	40.0	39.0	41.0	35.0	41.0
21	38.96525	40.0	38.0	41.0	35.0	41.0
22	39.109	40.0	39.0	41.0	36.0	41.0
23	38.986	40.0	39.0	41.0	36.0	41.0
24	38.938	40.0	39.0	41.0	35.0	41.0
25	39.2215	41.0	39.0	41.0	36.0	41.0
26	38.63725	40.0	38.0	41.0	35.0	41.0
27	38.7925	40.0	38.0	41.0	35.0	41.0
28	38.84525	40.0	38.0	41.0	35.0	41.0
29	38.5815	40.0	38.0	41.0	34.0	41.0
30	38.35075	40.0	38.0	41.0	34.0	41.0
31	38.6055	40.0	38.0	41.0	34.0	41.0
32	38.686	40.0	38.0	41.0	35.0	41.0
33	38.84575	40.0	38.0	41.0	35.0	41.0
34	38.76775	40.0	38.0	41.0	35.0	41.0
35	38.56575	40.0	38.0	41.0	34.0	41.0
36	38.123	40.0	38.0	41.0	34.0	41.0
37	37.7695	40.0	37.0	41.0	33.0	41.0
38	36.83025	39.0	37.0	41.0	30.0	41.0
39	37.44325	40.0	37.0	41.0	31.0	41.0
40	37.63825	40.0	37.0	41.0	33.0	41.0
41	37.91525	40.0	38.0	41.0	33.0	41.0
42	38.185	40.0	38.0	41.0	33.0	41.0
43	38.4705	40.0	38.0	41.0	34.0	41.0
44	38.10275	40.0	38.0	41.0	33.0	41.0
45	37.96425	40.0	38.0	41.0	33.0	41.0
46	37.89525	40.0	37.0	41.0	33.0	41.0
47	37.45625	40.0	37.0	41.0	32.0	41.0
48	37.16275	40.0	37.0	41.0	31.0	41.0
49	37.343	40.0	37.0	41.0	31.0	41.0
50	36.875	40.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	4.0
18	2.0
19	2.0
20	2.0
21	6.0
22	5.0
23	2.0
24	3.0
25	11.0
26	14.0
27	14.0
28	16.0
29	40.0
30	47.0
31	64.0
32	66.0
33	92.0
34	138.0
35	190.0
36	263.0
37	420.0
38	682.0
39	1916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.05	20.8	57.65	13.5
2	24.24924924924925	25.25025025025025	22.372372372372375	28.128128128128125
3	17.325	18.4	19.675	44.6
4	27.125	35.199999999999996	12.35	25.324999999999996
5	20.525	37.75	28.975	12.75
6	38.275	15.475	34.275	11.975
7	14.299999999999999	23.375	25.825	36.5
8	24.15	40.425	14.774999999999999	20.65
9	21.05	38.2	27.625	13.125
10	19.125	19.875	26.85	34.150000000000006
11	30.0	11.325000000000001	13.125	45.550000000000004
12	15.45	16.1	24.85	43.6
13	21.075	20.474999999999998	38.550000000000004	19.900000000000002
14	40.65	22.650000000000002	25.2	11.5
15	39.125	21.5	28.749999999999996	10.625
16	12.125	27.425	22.575	37.875
17	24.025	24.0	38.675	13.3
18	28.549999999999997	18.175	28.849999999999998	24.425
19	38.525	13.0	31.65	16.825000000000003
20	49.35	17.349999999999998	22.775000000000002	10.525
21	22.425	25.6	23.625	28.349999999999998
22	32.175	12.55	48.325	6.950000000000001
23	24.575	13.625000000000002	48.825	12.975
24	51.025	13.200000000000001	29.725	6.05
25	55.775	20.925	16.075	7.225
26	30.4	47.4	11.95	10.25
27	13.950000000000001	53.05	22.925	10.075000000000001
28	8.975	27.224999999999998	50.5	13.3
29	10.025	11.85	60.25	17.875
30	8.375	4.825	39.75	47.05
31	12.9	3.775	47.3	36.025
32	24.5	4.6	24.725	46.175
33	55.7	5.6000000000000005	14.274999999999999	24.425
34	67.975	6.3	12.65	13.075000000000001
35	69.39999999999999	6.950000000000001	16.225	7.425
36	42.875	7.3	38.75	11.075
37	46.975	8.95	23.65	20.424999999999997
38	24.875	14.05	13.05	48.025
39	14.274999999999999	21.25	9.2	55.275
40	12.375	49.125	8.975	29.525000000000002
41	22.225	54.574999999999996	8.3	14.899999999999999
42	50.425	32.775	8.075000000000001	8.725
43	58.375	26.275	6.175	9.175
44	33.475	48.5	6.7	11.325000000000001
45	17.45	54.50000000000001	10.65	17.4
46	10.8	28.425	16.950000000000003	43.824999999999996
47	7.725	13.375	39.324999999999996	39.574999999999996
48	8.799999999999999	11.3	22.75	57.15
49	11.425	18.099999999999998	12.625	57.85
50	16.45	40.275	10.100000000000001	33.175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	5.0
32	9.0
33	9.0
34	9.0
35	13.5
36	18.0
37	20.0
38	22.0
39	26.5
40	31.0
41	32.0
42	33.0
43	74.0
44	115.0
45	162.5
46	210.0
47	411.0
48	612.0
49	633.5
50	655.0
51	848.0
52	1041.0
53	679.5
54	318.0
55	348.5
56	379.0
57	320.0
58	261.0
59	157.0
60	53.0
61	50.5
62	48.0
63	63.0
64	78.0
65	48.5
66	19.0
67	13.0
68	7.0
69	12.5
70	18.0
71	35.0
72	52.0
73	27.0
74	2.0
75	1.5
76	1.0
77	1.5
78	2.0
79	1.5
80	1.0
81	2.5
82	4.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34443656980865	26.224999999999998
2	9.922041105598867	7.000000000000001
3	4.110559886605245	4.35
4	2.6222537207654146	3.6999999999999997
5	1.700921332388377	3.0
6	1.2756909992912826	2.7
7	0.7087172218284904	1.7500000000000002
8	0.4961020552799433	1.4000000000000001
9	0.3543586109142452	1.125
>10	3.8979447200566972	25.825
>50	0.28348688873139616	5.425
>100	0.28348688873139616	17.5
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	259	6.4750000000000005	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	180	4.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	113	2.825	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	56	1.4000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	55	1.375	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	47	1.175	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	39	0.975	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	38	0.95	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	25	0.625	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	23	0.575	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC	19	0.475	RNA PCR Primer, Index 25 (100% over 50bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	14	0.35000000000000003	No Hit
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC	14	0.35000000000000003	RNA PCR Primer, Index 5 (100% over 35bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	12	0.3	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	11	0.27499999999999997	No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	11	0.27499999999999997	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	10	0.25	No Hit
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	8	0.2	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 34bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC	6	0.15	RNA PCR Primer, Index 25 (100% over 44bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	5	0.125	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
TGTAAACATCCTCGACTGGAAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTCGCTCTGAAGGCCTGTTTCCTAGGCTTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.5	0.0	0.0	0.0
2	0.0	0.5	0.0	0.0	0.0
3	0.0	0.575	0.0	0.0	0.0
4	0.0	0.575	0.0	0.0	0.0
5	0.0	0.6	0.0	0.0	0.0
6	0.0	0.625	0.0	0.0	0.0
7	0.0	0.8	0.0	0.0	0.0
8	0.0	0.95	0.0	0.0	0.0
9	0.0	1.025	0.0	0.0	0.0
10	0.0	1.125	0.0	0.0	0.0
11	0.0	1.325	0.0	0.0	0.0
12	0.0	1.5	0.0	0.0	0.0
13	0.0	1.825	0.0	0.0	0.0
14	0.0	2.05	0.0	0.0	0.0
15	0.0	2.4	0.0	0.0	0.0
16	0.0	3.125	0.0	0.0	0.0
17	0.0	3.875	0.0	0.0	0.0
18	0.0	4.675	0.0	0.0	0.0
19	0.0	5.175	0.0	0.0	0.0
20	0.0	6.275	0.0	0.0	0.0
21	0.0	11.075	0.0	0.0	0.0
22	0.0	21.675	0.0	0.0	0.0
23	0.0	54.325	0.0	0.0	0.0
24	0.0	71.175	0.0	0.0	0.0
25	0.0	77.85	0.0	0.0	0.0
26	0.0	78.8	0.0	0.0	0.0
27	0.0	79.325	0.0	0.0	0.0
28	0.0	79.875	0.0	0.0	0.0
29	0.0	80.425	0.0	0.0	0.0
30	0.0	80.95	0.0	0.0	0.0
31	0.0	82.1	0.0	0.0	0.0
32	0.0	83.65	0.0	0.0	0.0
33	0.0	85.175	0.0	0.0	0.0
34	0.0	87.6	0.0	0.0	0.0
35	0.0	88.775	0.0	0.0	0.0
36	0.0	89.55	0.0	0.0	0.0
37	0.0	90.275	0.0	0.0	0.0
38	0.0	90.775	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	45	2.728484E-10	44.000004	8
GGTGAGT	45	2.728484E-10	44.000004	17
TCAAGTA	45	2.728484E-10	44.000004	2
ACGCTGT	45	2.728484E-10	44.000004	9
AATCCAG	40	5.315087E-9	44.000004	8
TAAACAT	20	6.952051E-4	44.000004	3
ATCCAGG	40	5.315087E-9	44.000004	9
TTGTATA	20	6.952051E-4	44.000004	13
AAACATC	20	6.952051E-4	44.000004	4
CCTGTTG	20	6.952051E-4	44.000004	18
TTCAAGT	45	2.728484E-10	44.000004	1
TTCAACG	45	2.728484E-10	44.000004	5
AGGATAG	45	2.728484E-10	44.000004	13
ATAGGCT	40	5.315087E-9	44.000004	16
TCAACGC	45	2.728484E-10	44.000004	6
TCCAGGA	40	5.315087E-9	44.000004	10
ACCTGTT	20	6.952051E-4	44.000004	17
GGATAGG	45	2.728484E-10	44.000004	14
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGTCG	45	2.728484E-10	44.000004	11
>>END_MODULE
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
Read 418515 spots for SRR5031053.sra
Written 418515 spots for SRR5031053.sra
Read 418507 spots for SRR5031053.sra
Written 418507 spots for SRR5031053.sra
SRR ids: ['SRR5031053.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tg65ogso
SRR5031053.sra spots: 5022092
blocks: [[1, 418507], [418508, 837014], [837015, 1255521], [1255522, 1674028], [1674029, 2092535], [2092536, 2511042], [2511043, 2929549], [2929550, 3348056], [3348057, 3766563], [3766564, 4185070], [4185071, 4603577], [4603578, 5022092]]
SRR5031053 file size 869390
SRR5031053 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031053 SRR5031053_1.fastq
Input file:	SRR5031053_1.fastq
trimmed:	SRR5031053-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:14:48 2020 >> started

Wed Mar  4 07:14:51 2020 >> done (2.886s)
5022092 reads processed; of these:
    326 ( 0.01%) short reads filtered out after trimming by size control
     15 ( 0.00%) empty reads filtered out after trimming by size control
5021751 (99.99%) reads available; of these:
 238015 ( 4.74%) trimmed reads available after processing
4783736 (95.26%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     85	  0.00%
 19	     99	  0.00%
 20	    235	  0.00%
 21	    316	  0.01%
 22	    576	  0.01%
 23	    686	  0.01%
 24	    608	  0.01%
 25	    884	  0.02%
 26	    689	  0.01%
 27	    900	  0.02%
 28	   1001	  0.02%
 29	   1979	  0.04%
 30	    991	  0.02%
 31	   1055	  0.02%
 32	   1092	  0.02%
 33	   1781	  0.04%
 34	   2422	  0.05%
 35	   5615	  0.11%
 36	   4257	  0.08%
 37	   4754	  0.09%
 38	   3914	  0.08%
 39	   4020	  0.08%
 40	   4667	  0.09%
 41	   4742	  0.09%
 42	   6002	  0.12%
 43	   8668	  0.17%
 44	  11599	  0.23%
 45	  15586	  0.31%
 46	  21027	  0.42%
 47	  29276	  0.58%
 48	  48971	  0.98%
 49	  49518	  0.99%
 50	4783736	 95.26%
5021751 reads passed initial QC


criterion=sequence-density
sequence-density=90.94
sequence-density-rank=1
fanout-score=19.62
fanout-score-rank=1
prefix-density=92.30
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC


criterion=fanout-score
sequence-density=90.94
sequence-density-rank=1
fanout-score=19.62
fanout-score-rank=1
prefix-density=92.30
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC -o SRR5031053 -
Input file:	STDIN
trimmed:	SRR5031053-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:15:17 2020 >> started

Wed Mar  4 07:15:23 2020 >> done (6.477s)
4911383 reads processed; of these:
 189917 ( 3.87%) short reads filtered out after trimming by size control
  26662 ( 0.54%) empty reads filtered out after trimming by size control
4694804 (95.59%) reads available; of these:
4555749 (97.04%) trimmed reads available after processing
 139055 ( 2.96%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  24288	  0.52%
 19	  50996	  1.09%
 20	 228943	  4.88%
 21	 558352	 11.89%
 22	1723090	 36.70%
 23	 862539	 18.37%
 24	 294886	  6.28%
 25	  52483	  1.12%
 26	  26140	  0.56%
 27	  25205	  0.54%
 28	  29466	  0.63%
 29	  26795	  0.57%
 30	  46777	  1.00%
 31	  59627	  1.27%
 32	  66868	  1.42%
 33	 100913	  2.15%
 34	  58367	  1.24%
 35	  34298	  0.73%
 36	  40230	  0.86%
 37	  20177	  0.43%
 38	  23576	  0.50%
 39	  19785	  0.42%
 40	  26486	  0.56%
 41	  35708	  0.76%
 42	  35815	  0.76%
 43	  25330	  0.54%
 44	  20227	  0.43%
 45	  25069	  0.53%
 46	  17762	  0.38%
 47	  13377	  0.28%
 48	   1337	  0.03%
 49	   1906	  0.04%
 50	 117986	  2.51%


criterion=sequence-density
sequence-density=3.47
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=TGTAAACATCCTCGACTGGAAGC


criterion=fanout-score
sequence-density=0.40
sequence-density-rank=34
fanout-score=15.13
fanout-score-rank=1
prefix-density=6.03
prefix-fanout=1.0
sequence=TGAGCTTGGCTATT
                                 Started job on |	Mar 04 07:15:48
                             Started mapping on |	Mar 04 07:15:49
                                    Finished on |	Mar 04 07:16:41
       Mapping speed, Million of reads per hour |	332.67

                          Number of input reads |	4805172
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2362968
                        Uniquely mapped reads % |	49.18%
                          Average mapped length |	23.86
                       Number of splices: Total |	3046
            Number of splices: Annotated (sjdb) |	379
                       Number of splices: GT/AG |	2836
                       Number of splices: GC/AG |	40
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	170
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1993557
             % of reads mapped to multiple loci |	41.49%
        Number of reads mapped to too many loci |	94887
             % of reads mapped to too many loci |	1.97%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.79%
                     % of reads unmapped: other |	0.57%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	448647	448647	448647
N_multimapping	1993557	1993557	1993557
N_noFeature	99198	122357	1765967
N_ambiguous	1318903	801366	13969
UnstrandedReadsAssigned:944867 PositiveStrandReadsAssigned:1439245 NegativeStrandReadsAssigned:583032
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031053 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031053-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,805,172 reads, 1,039,133 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 728 rounds

 109283 SRR5031053.ke.tsv
  52636 SRR5031053.se.tsv
 161919 total
==> SRR5031053.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031053.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	1
ENSMUSG00000103147	0
SRR5031053 completed mapping pipeline successfully
