Starting /dee2/code/volunteer_pipeline.sh SRR5030882
    current disk space = 160636125184
    free memory = 73945728 
SRR5030882 SRAfilesize
e91d00ae2a1139edf38596dc10157096  SRR5030882.sra
SRR5030882.sra file validated
SRR5030882 is single end
SRR5030882 is conventional basespace
SRR5030882 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3905	33.0	31.0	34.0	31.0	34.0
2	32.7305	34.0	31.0	34.0	31.0	34.0
3	32.74175	34.0	31.0	34.0	31.0	34.0
4	36.1675	37.0	37.0	37.0	35.0	37.0
5	36.2205	37.0	37.0	37.0	35.0	37.0
6	35.934	37.0	35.0	37.0	35.0	37.0
7	36.06375	37.0	35.0	37.0	35.0	37.0
8	36.13475	37.0	35.0	37.0	35.0	37.0
9	37.81175	39.0	38.0	39.0	35.0	39.0
10	37.872	39.0	38.0	39.0	35.0	39.0
11	37.91125	39.0	38.0	39.0	35.0	39.0
12	37.839	39.0	38.0	39.0	35.0	39.0
13	37.84725	39.0	38.0	39.0	35.0	39.0
14	39.23975	41.0	39.0	41.0	36.0	41.0
15	39.19	41.0	39.0	41.0	36.0	41.0
16	39.0625	40.0	38.0	41.0	36.0	41.0
17	39.173	40.0	38.0	41.0	36.0	41.0
18	39.19775	41.0	39.0	41.0	36.0	41.0
19	38.94125	40.0	38.0	41.0	35.0	41.0
20	39.07725	40.0	39.0	41.0	36.0	41.0
21	39.093	40.0	39.0	41.0	35.0	41.0
22	39.09925	40.0	39.0	41.0	36.0	41.0
23	39.108	40.0	39.0	41.0	36.0	41.0
24	38.50175	40.0	38.0	41.0	34.0	41.0
25	38.6745	40.0	38.0	41.0	34.0	41.0
26	38.70625	40.0	38.0	41.0	34.0	41.0
27	38.808	40.0	38.0	41.0	35.0	41.0
28	38.64775	40.0	38.0	41.0	35.0	41.0
29	38.4215	40.0	38.0	41.0	34.0	41.0
30	38.1575	40.0	38.0	41.0	33.0	41.0
31	38.55725	40.0	38.0	41.0	34.0	41.0
32	38.776	40.0	38.0	41.0	35.0	41.0
33	37.09475	40.0	37.0	41.0	30.0	41.0
34	36.62625	40.0	37.0	41.0	30.0	41.0
35	32.65	38.0	30.0	40.0	15.0	41.0
36	29.60075	37.0	19.0	40.0	8.0	41.0
37	29.744	36.0	23.0	39.0	7.0	41.0
38	32.41575	37.0	30.0	40.0	16.0	41.0
39	31.941	37.0	30.0	40.0	9.0	41.0
40	32.95225	37.0	32.0	40.0	9.0	41.0
41	30.3085	36.0	26.0	40.0	2.0	41.0
42	31.942	37.0	31.0	40.0	2.0	41.0
43	30.05375	36.0	26.0	40.0	2.0	41.0
44	31.0695	37.0	30.0	40.0	2.0	41.0
45	29.0815	36.0	24.0	40.0	2.0	41.0
46	27.25675	35.0	14.0	39.0	2.0	41.0
47	26.73125	34.0	13.0	39.0	2.0	40.0
48	27.5335	35.0	14.0	39.0	2.0	41.0
49	25.61375	34.0	2.0	39.0	2.0	41.0
50	22.50275	30.0	2.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	1.0
16	1.0
17	3.0
18	3.0
19	3.0
20	12.0
21	7.0
22	14.0
23	29.0
24	55.0
25	64.0
26	87.0
27	142.0
28	71.0
29	119.0
30	172.0
31	179.0
32	165.0
33	176.0
34	239.0
35	269.0
36	322.0
37	456.0
38	636.0
39	772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.331165582791396	25.987993996998497	44.847423711855924	16.833416708354175
2	26.775	25.650000000000002	19.925	27.650000000000002
3	22.425	21.175	22.55	33.85
4	31.95	28.050000000000004	16.400000000000002	23.599999999999998
5	23.799999999999997	38.7	25.85	11.65
6	30.225	15.775	37.525	16.475
7	20.349999999999998	20.849999999999998	22.900000000000002	35.9
8	25.124999999999996	30.275000000000002	18.2	26.400000000000002
9	18.35	32.975	30.425	18.25
10	24.0	22.425	27.175	26.400000000000002
11	37.075	13.5	15.7	33.725
12	18.125	23.45	27.650000000000002	30.775000000000002
13	26.6	18.825	35.6	18.975
14	28.275	27.525	24.05	20.150000000000002
15	33.35	23.875	25.724999999999998	17.05
16	20.875	19.75	24.25	35.125
17	25.224999999999998	25.7	34.475	14.6
18	24.5	18.15	30.125	27.224999999999998
19	33.875	15.65	31.85	18.625
20	40.275	16.150000000000002	28.325	15.25
21	24.75	21.15	27.375	26.724999999999998
22	29.875	12.950000000000001	43.275000000000006	13.900000000000002
23	26.375	14.499999999999998	41.8	17.325
24	50.6	13.925	24.275	11.200000000000001
25	47.099999999999994	23.05	15.425	14.424999999999999
26	27.450000000000003	42.475	14.774999999999999	15.299999999999999
27	17.150000000000002	43.6	22.075	17.175
28	14.524999999999999	24.224999999999998	44.324999999999996	16.925
29	14.45	14.325	52.6	18.625
30	12.475	8.674999999999999	33.175	45.675
31	14.224999999999998	7.55	45.925	32.300000000000004
32	28.675	7.124999999999999	22.35	41.85
33	49.0	12.049999999999999	14.924999999999999	24.025
34	56.425000000000004	7.95	18.125	17.5
35	56.425000000000004	7.95	20.8	14.825
36	60.975	9.0	16.425	13.600000000000001
37	37.675	14.374999999999998	21.6	26.35
38	25.35	17.25	13.575000000000001	43.824999999999996
39	18.475	24.275	13.825000000000001	43.425000000000004
40	17.375	44.975	14.149999999999999	23.5
41	26.75	40.775	12.525	19.950000000000003
42	47.025	26.724999999999998	14.249999999999998	12.0
43	41.925000000000004	32.550000000000004	12.225	13.3
44	30.5	44.6	9.75	15.15
45	22.575	43.2	11.725	22.5
46	16.775000000000002	27.175	24.2	31.85
47	12.075	16.225	34.525	37.175000000000004
48	14.85	12.125	21.65	51.37500000000001
49	16.400000000000002	24.85	14.149999999999999	44.6
50	29.9	24.0	11.825	34.275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	2.0
20	2.0
21	1.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	3.0
30	4.0
31	6.5
32	9.0
33	15.5
34	22.0
35	37.5
36	53.0
37	63.5
38	74.0
39	73.5
40	73.0
41	66.0
42	59.0
43	90.0
44	121.0
45	197.5
46	274.0
47	287.0
48	300.0
49	375.0
50	450.0
51	613.0
52	776.0
53	632.0
54	488.0
55	425.5
56	363.0
57	324.0
58	285.0
59	223.0
60	161.0
61	132.0
62	103.0
63	148.5
64	194.0
65	117.0
66	40.0
67	27.0
68	14.0
69	21.0
70	28.0
71	48.0
72	68.0
73	41.0
74	14.0
75	9.0
76	4.0
77	5.0
78	6.0
79	6.0
80	6.0
81	4.5
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63397721644378	40.699999999999996
2	8.122833085685983	8.200000000000001
3	3.2194155522535906	4.875
4	1.8325903912828134	3.6999999999999997
5	1.4363546310054482	3.6249999999999996
6	0.44576523031203563	1.35
7	0.5448241703813769	1.925
8	0.3467062902426944	1.4000000000000001
9	0.3467062902426944	1.575
>10	2.922238732045567	25.5
>50	0.04952947003467063	1.4500000000000002
>100	0.09905894006934125	5.7
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	117	2.9250000000000003	No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	111	2.775	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	30	0.75	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (96% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (96% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	26	0.65	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	26	0.65	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	21	0.525	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC	21	0.525	RNA PCR Primer, Index 6 (100% over 50bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	21	0.525	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	17	0.42500000000000004	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	16	0.4	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC	16	0.4	RNA PCR Primer, Index 5 (97% over 35bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC	15	0.375	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	15	0.375	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	15	0.375	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	15	0.375	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC	13	0.325	RNA PCR Primer, Index 6 (100% over 44bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	12	0.3	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	10	0.25	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	10	0.25	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCG	10	0.25	RNA PCR Primer, Index 1 (96% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	10	0.25	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCG	10	0.25	RNA PCR Primer, Index 1 (96% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	9	0.22499999999999998	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	9	0.22499999999999998	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	9	0.22499999999999998	No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	8	0.2	RNA PCR Primer, Index 1 (96% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	8	0.2	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	8	0.2	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	7	0.17500000000000002	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	7	0.17500000000000002	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	7	0.17500000000000002	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	6	0.15	No Hit
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TTTGCATGATGACTTGAATTGTCGGATACCCCTTCACCCCGTTCATGGGT	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGTGCCAAGGAACTCCAG	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAATTCCA	5	0.125	RNA PCR Primer, Index 1 (95% over 23bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.55	0.0	0.0	0.0
2	0.0	0.575	0.0	0.0	0.0
3	0.0	0.625	0.0	0.0	0.0
4	0.0	0.625	0.0	0.0	0.0
5	0.0	0.65	0.0	0.0	0.0
6	0.0	0.7	0.0	0.0	0.0
7	0.0	1.1	0.0	0.0	0.0
8	0.0	1.15	0.0	0.0	0.0
9	0.0	1.25	0.0	0.0	0.0
10	0.0	1.325	0.0	0.0	0.0
11	0.0	1.35	0.0	0.0	0.0
12	0.0	1.525	0.0	0.0	0.0
13	0.0	1.7	0.0	0.0	0.0
14	0.0	1.9	0.0	0.0	0.0
15	0.0	2.275	0.0	0.0	0.0
16	0.0	3.15	0.0	0.0	0.0
17	0.0	3.575	0.0	0.0	0.0
18	0.0	4.55	0.0	0.0	0.0
19	0.0	4.95	0.0	0.0	0.0
20	0.0	5.6	0.0	0.0	0.0
21	0.0	9.575	0.0	0.0	0.0
22	0.0	18.95	0.0	0.0	0.0
23	0.0	44.775	0.0	0.0	0.0
24	0.0	56.125	0.0	0.0	0.0
25	0.0	61.125	0.0	0.0	0.0
26	0.0	62.825	0.0	0.0	0.0
27	0.0	63.45	0.0	0.0	0.0
28	0.0	64.15	0.0	0.0	0.0
29	0.0	64.375	0.0	0.0	0.0
30	0.0	64.825	0.0	0.0	0.0
31	0.0	65.725	0.0	0.0	0.0
32	0.0	66.55	0.0	0.0	0.0
33	0.0	67.625	0.0	0.0	0.0
34	0.0	72.125	0.0	0.0	0.0
35	0.0	73.275	0.0	0.0	0.0
36	0.0	73.9	0.0	0.0	0.0
37	0.0	75.175	0.0	0.0	0.0
38	0.0	75.675	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
TGCACTC	20	6.952051E-4	44.000004	4
GAGGTAG	20	6.952051E-4	44.000004	2
ACTCGTC	20	6.952051E-4	44.000004	7
TTGCACT	20	6.952051E-4	44.000004	3
ACGCTGT	20	6.952051E-4	44.000004	9
TTCAACG	20	6.952051E-4	44.000004	5
CCCGGCC	20	6.952051E-4	44.000004	13
TCAACGC	20	6.952051E-4	44.000004	6
TATTGCA	20	6.952051E-4	44.000004	1
GTCCCGG	20	6.952051E-4	44.000004	11
ATTGCAC	20	6.952051E-4	44.000004	2
GGCTTGG	20	6.952051E-4	44.000004	19
CACTCGT	20	6.952051E-4	44.000004	6
TCGTCCC	20	6.952051E-4	44.000004	9
TGAGGTA	20	6.952051E-4	44.000004	1
TCCCGGC	20	6.952051E-4	44.000004	12
GCACTCG	20	6.952051E-4	44.000004	5
AGGTAGT	20	6.952051E-4	44.000004	3
AATGATG	20	6.952051E-4	44.000004	4
>>END_MODULE
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204088 spots for SRR5030882.sra
Written 204088 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
Read 204085 spots for SRR5030882.sra
Written 204085 spots for SRR5030882.sra
SRR ids: ['SRR5030882.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_molxvyoo
SRR5030882.sra spots: 2449023
blocks: [[1, 204085], [204086, 408170], [408171, 612255], [612256, 816340], [816341, 1020425], [1020426, 1224510], [1224511, 1428595], [1428596, 1632680], [1632681, 1836765], [1836766, 2040850], [2040851, 2244935], [2244936, 2449023]]
SRR5030882 file size 423391
SRR5030882 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030882 SRR5030882_1.fastq
Input file:	SRR5030882_1.fastq
trimmed:	SRR5030882-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:36:09 2020 >> started

Wed Mar  4 06:36:10 2020 >> done (1.501s)
2449023 reads processed; of these:
    144 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
2448875 (99.99%) reads available; of these:
 282104 (11.52%) trimmed reads available after processing
2166771 (88.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     41	  0.00%
 19	     45	  0.00%
 20	     91	  0.00%
 21	    143	  0.01%
 22	    243	  0.01%
 23	    300	  0.01%
 24	    313	  0.01%
 25	    468	  0.02%
 26	    376	  0.02%
 27	    480	  0.02%
 28	    712	  0.03%
 29	    971	  0.04%
 30	    674	  0.03%
 31	   1005	  0.04%
 32	   2618	  0.11%
 33	   7816	  0.32%
 34	  19288	  0.79%
 35	   4421	  0.18%
 36	   4902	  0.20%
 37	   2809	  0.11%
 38	   5470	  0.22%
 39	   5379	  0.22%
 40	  10531	  0.43%
 41	   5631	  0.23%
 42	  15530	  0.63%
 43	   6272	  0.26%
 44	  26509	  1.08%
 45	  18482	  0.75%
 46	  17771	  0.73%
 47	  18469	  0.75%
 48	  51199	  2.09%
 49	  53145	  2.17%
 50	2166771	 88.48%
2448875 reads passed initial QC


criterion=sequence-density
sequence-density=74.19
sequence-density-rank=1
fanout-score=21.73
fanout-score-rank=3
prefix-density=76.93
prefix-fanout=21.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC


criterion=fanout-score
sequence-density=1.52
sequence-density-rank=6
fanout-score=472.08
fanout-score-rank=1
prefix-density=76.10
prefix-fanout=9.5
sequence=GGAATTCTCGGTGCCAAGGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC -o SRR5030882 -
Input file:	STDIN
trimmed:	SRR5030882-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:36:23 2020 >> started

Wed Mar  4 06:36:26 2020 >> done (3.011s)
2383572 reads processed; of these:
 101392 ( 4.25%) short reads filtered out after trimming by size control
  12842 ( 0.54%) empty reads filtered out after trimming by size control
2269338 (95.21%) reads available; of these:
1871090 (82.45%) trimmed reads available after processing
 398248 (17.55%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12496	  0.55%
 19	  20491	  0.90%
 20	  91162	  4.02%
 21	 230811	 10.17%
 22	 591667	 26.07%
 23	 296715	 13.07%
 24	 127327	  5.61%
 25	  33287	  1.47%
 26	  17659	  0.78%
 27	  15447	  0.68%
 28	  15834	  0.70%
 29	  14107	  0.62%
 30	  18918	  0.83%
 31	  21707	  0.96%
 32	  23779	  1.05%
 33	 104179	  4.59%
 34	  25885	  1.14%
 35	  18621	  0.82%
 36	  26772	  1.18%
 37	  12331	  0.54%
 38	  14670	  0.65%
 39	  13776	  0.61%
 40	  19855	  0.87%
 41	  23406	  1.03%
 42	  29599	  1.30%
 43	  20295	  0.89%
 44	  20237	  0.89%
 45	  22389	  0.99%
 46	  18236	  0.80%
 47	  15797	  0.70%
 48	   5775	  0.25%
 49	  10623	  0.47%
 50	 335485	 14.78%


criterion=sequence-density
sequence-density=6.87
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.46
sequence-density-rank=30
fanout-score=11.83
fanout-score-rank=1
prefix-density=5.45
prefix-fanout=1.0
sequence=TGAGCTTGGCTATTT
                                 Started job on |	Mar 04 06:36:47
                             Started mapping on |	Mar 04 06:36:47
                                    Finished on |	Mar 04 06:37:10
       Mapping speed, Million of reads per hour |	365.42

                          Number of input reads |	2334641
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1166113
                        Uniquely mapped reads % |	49.95%
                          Average mapped length |	28.74
                       Number of splices: Total |	1476
            Number of splices: Annotated (sjdb) |	215
                       Number of splices: GT/AG |	1373
                       Number of splices: GC/AG |	17
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	85
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.36
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.11
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	748980
             % of reads mapped to multiple loci |	32.08%
        Number of reads mapped to too many loci |	58539
             % of reads mapped to too many loci |	2.51%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.97%
                     % of reads unmapped: other |	0.49%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	419548	419548	419548
N_multimapping	748980	748980	748980
N_noFeature	60158	74837	869614
N_ambiguous	627972	372283	13528
UnstrandedReadsAssigned:477983 PositiveStrandReadsAssigned:718993 NegativeStrandReadsAssigned:282971
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030882 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030882-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,334,641 reads, 525,503 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,080 rounds

 109283 SRR5030882.ke.tsv
  52636 SRR5030882.se.tsv
 161919 total
==> SRR5030882.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030882.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030882 completed mapping pipeline successfully
