Starting /dee2/code/volunteer_pipeline.sh SRR5031089
    current disk space = 160484999168
    free memory = 73936584 
SRR5031089 SRAfilesize
3b1d43de0774934558e464a1f245304d  SRR5031089.sra
SRR5031089.sra file validated
SRR5031089 is single end
SRR5031089 is conventional basespace
SRR5031089 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80275	31.0	31.0	34.0	30.0	34.0
2	32.2945	33.0	31.0	34.0	31.0	34.0
3	32.37625	33.0	31.0	34.0	31.0	34.0
4	36.12375	37.0	35.0	37.0	35.0	37.0
5	36.06925	37.0	35.0	37.0	35.0	37.0
6	36.046	37.0	35.0	37.0	35.0	37.0
7	35.7875	37.0	35.0	37.0	35.0	37.0
8	35.5825	37.0	35.0	37.0	33.0	37.0
9	36.81975	39.0	37.0	39.0	33.0	39.0
10	36.57475	39.0	37.0	39.0	32.0	39.0
11	37.31025	39.0	37.0	39.0	34.0	39.0
12	37.6095	39.0	37.0	39.0	35.0	39.0
13	37.3955	39.0	37.0	39.0	35.0	39.0
14	38.2635	40.0	38.0	41.0	33.0	41.0
15	36.98625	40.0	37.0	41.0	30.0	41.0
16	36.958	39.0	36.0	40.0	31.0	41.0
17	36.91725	39.0	36.0	41.0	32.0	41.0
18	38.0525	39.0	37.0	41.0	33.0	41.0
19	38.58275	40.0	38.0	41.0	34.0	41.0
20	38.14425	40.0	38.0	41.0	34.0	41.0
21	38.486	40.0	38.0	41.0	34.0	41.0
22	37.164	39.0	37.0	40.0	31.0	41.0
23	37.67675	39.0	37.0	40.0	33.0	41.0
24	36.77425	39.0	36.0	40.0	30.0	41.0
25	37.7035	39.0	37.0	40.0	32.0	41.0
26	37.66075	39.0	37.0	40.0	32.0	41.0
27	37.683	40.0	37.0	40.0	33.0	41.0
28	38.24275	40.0	38.0	41.0	34.0	41.0
29	38.03775	40.0	38.0	41.0	33.0	41.0
30	38.48075	40.0	38.0	41.0	34.0	41.0
31	38.5635	40.0	38.0	41.0	35.0	41.0
32	38.624	40.0	38.0	41.0	35.0	41.0
33	38.422	40.0	38.0	41.0	35.0	41.0
34	37.6925	40.0	37.0	41.0	32.0	41.0
35	37.21975	40.0	36.0	41.0	30.0	41.0
36	33.417	35.0	30.0	39.0	23.0	41.0
37	35.24075	37.0	33.0	39.0	30.0	40.0
38	36.381	38.0	35.0	39.0	30.0	40.0
39	36.693	38.0	36.0	40.0	31.0	40.0
40	37.126	39.0	37.0	40.0	32.0	41.0
41	37.348	39.0	37.0	40.0	32.0	41.0
42	37.40775	40.0	37.0	40.0	33.0	41.0
43	37.56275	40.0	37.0	41.0	33.0	41.0
44	37.00325	39.0	36.0	40.0	31.0	41.0
45	36.95025	39.0	36.0	40.0	31.0	41.0
46	36.767	39.0	36.0	40.0	31.0	41.0
47	35.6235	38.0	34.0	40.0	28.0	41.0
48	36.7175	39.0	36.0	40.0	31.0	41.0
49	36.97925	39.0	37.0	40.0	31.0	41.0
50	35.045	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	2.0
17	3.0
18	1.0
19	2.0
20	5.0
21	5.0
22	5.0
23	3.0
24	8.0
25	14.0
26	12.0
27	19.0
28	21.0
29	36.0
30	43.0
31	64.0
32	105.0
33	134.0
34	191.0
35	301.0
36	530.0
37	800.0
38	1084.0
39	610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.580290145072537	26.388194097048522	48.44922461230615	14.582291145572787
2	24.2864296444667	28.768152228342515	19.128693039559337	27.81672508763145
3	24.875	18.9	22.625	33.6
4	33.775	31.974999999999998	13.100000000000001	21.15
5	20.525	35.35	30.3	13.825000000000001
6	26.775	16.150000000000002	42.125	14.95
7	18.325	20.75	21.5	39.425
8	24.175	30.099999999999998	17.45	28.275
9	25.1	31.175000000000004	27.200000000000003	16.525000000000002
10	25.75	16.125	28.65	29.475
11	35.05	11.075	18.45	35.425000000000004
12	17.625	17.724999999999998	33.225	31.424999999999997
13	29.2	15.825	34.775	20.200000000000003
14	33.074999999999996	28.7	20.424999999999997	17.8
15	40.325	14.075	27.900000000000002	17.7
16	18.224999999999998	15.675	27.500000000000004	38.6
17	32.2	18.025	34.775	15.0
18	32.15	15.15	30.099999999999998	22.6
19	34.975	16.900000000000002	33.725	14.399999999999999
20	47.8	15.575	23.175	13.450000000000001
21	23.9	23.9	26.674999999999997	25.525
22	32.2	10.9	45.425	11.475
23	26.25	13.675	44.55	15.525
24	46.6	12.475	28.225	12.7
25	47.875	23.175	18.025	10.925
26	30.5	42.225	16.1	11.175
27	18.45	43.725	25.124999999999996	12.7
28	15.024999999999999	24.425	45.324999999999996	15.225
29	14.424999999999999	10.9	53.175	21.5
30	12.475	6.4750000000000005	36.325	44.725
31	15.475	5.525	42.75	36.25
32	27.775	6.9750000000000005	22.2	43.05
33	51.849999999999994	9.35	14.05	24.75
34	59.724999999999994	10.35	15.024999999999999	14.899999999999999
35	64.67500000000001	8.875	17.724999999999998	8.725
36	43.85	8.575000000000001	33.85	13.725000000000001
37	43.575	12.45	19.400000000000002	24.575
38	22.925	20.45	12.225	44.4
39	14.325	26.825	9.975000000000001	48.875
40	15.174999999999999	47.599999999999994	11.05	26.174999999999997
41	24.95	46.800000000000004	9.45	18.8
42	44.95	30.675	10.15	14.224999999999998
43	47.8	31.424999999999997	7.725	13.05
44	29.75	46.025	7.575	16.650000000000002
45	18.7	47.525	14.625	19.15
46	13.200000000000001	24.8	18.725	43.275000000000006
47	9.225	17.2	35.4	38.175
48	10.325	15.174999999999999	19.475	55.025
49	14.774999999999999	22.35	13.4	49.475
50	19.925	35.85	12.875	31.35
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	2.0
32	4.0
33	4.5
34	5.0
35	7.5
36	10.0
37	13.5
38	17.0
39	20.0
40	23.0
41	28.0
42	33.0
43	74.5
44	116.0
45	135.0
46	154.0
47	282.5
48	411.0
49	504.5
50	598.0
51	774.0
52	950.0
53	673.0
54	396.0
55	489.5
56	583.0
57	462.0
58	341.0
59	226.5
60	112.0
61	86.5
62	61.0
63	55.0
64	49.0
65	43.0
66	37.0
67	28.5
68	20.0
69	20.0
70	20.0
71	36.0
72	52.0
73	30.0
74	8.0
75	4.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78782802903406	35.725
2	8.263539921831379	7.3999999999999995
3	3.796761585706309	5.1
4	1.340033500837521	2.4
5	1.340033500837521	3.0
6	0.7816862088218872	2.1
7	0.8375209380234505	2.625
8	0.5025125628140703	1.7999999999999998
9	0.33500837520938026	1.35
>10	2.512562814070352	18.35
>50	0.33500837520938026	10.25
>100	0.16750418760469013	9.9
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	149	3.7249999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	132	3.3000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	96	2.4	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	76	1.9	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG	74	1.8499999999999999	RNA PCR Primer, Index 2 (97% over 35bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	60	1.5	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	49	1.225	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	38	0.95	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC	35	0.8750000000000001	RNA PCR Primer, Index 13 (100% over 50bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	22	0.5499999999999999	No Hit
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (96% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT	19	0.475	RNA PCR Primer, Index 8 (97% over 36bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC	18	0.44999999999999996	RNA PCR Primer, Index 13 (100% over 44bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	17	0.42500000000000004	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG	13	0.325	RNA PCR Primer, Index 2 (97% over 35bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	11	0.27499999999999997	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	10	0.25	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	10	0.25	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	10	0.25	RNA PCR Primer, Index 1 (100% over 24bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	8	0.2	No Hit
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 34bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG	7	0.17500000000000002	RNA PCR Primer, Index 13 (100% over 45bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGACTTCTCGGGTGCCAAGGAACTCCAGTCAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC	7	0.17500000000000002	RNA PCR Primer, Index 13 (100% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TGAGGTAGTAGATTGTCTAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	6	0.15	RNA PCR Primer, Index 1 (100% over 34bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA	6	0.15	RNA PCR Primer, Index 13 (100% over 39bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	6	0.15	No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACAGTCAAATCTCGTATGC	5	0.125	RNA PCR Primer, Index 13 (98% over 50bp)
AGGCAGTGTAGTTAGCTGATTGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGACTTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (96% over 26bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT	5	0.125	RNA PCR Primer, Index 8 (97% over 36bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTAT	5	0.125	RNA PCR Primer, Index 13 (100% over 48bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGGCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTCCGCCTGTCTGAGCGTCGCTTG	5	0.125	No Hit
CATGCTCAATAGGATTACGCTGAGGCCCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.3	0.0	0.0	0.0
2	0.0	1.45	0.0	0.0	0.0
3	0.0	1.65	0.0	0.0	0.0
4	0.0	1.725	0.0	0.0	0.0
5	0.0	1.775	0.0	0.0	0.0
6	0.0	2.025	0.0	0.0	0.0
7	0.0	2.8	0.0	0.0	0.0
8	0.0	3.05	0.0	0.0	0.0
9	0.0	3.2	0.0	0.0	0.0
10	0.0	3.575	0.0	0.0	0.0
11	0.0	3.8	0.0	0.0	0.0
12	0.0	4.2	0.0	0.0	0.0
13	0.0	4.575	0.0	0.0	0.0
14	0.0	5.15	0.0	0.0	0.0
15	0.0	6.1	0.0	0.0	0.0
16	0.0	8.825	0.0	0.0	0.0
17	0.0	10.0	0.0	0.0	0.0
18	0.0	11.5	0.0	0.0	0.0
19	0.0	11.975	0.0	0.0	0.0
20	0.0	13.2	0.0	0.0	0.0
21	0.0	17.85	0.0	0.0	0.0
22	0.0	28.775	0.0	0.0	0.0
23	0.0	55.875	0.0	0.0	0.0
24	0.0	68.625	0.0	0.0	0.0
25	0.0	73.675	0.0	0.0	0.0
26	0.0	75.175	0.0	0.0	0.0
27	0.0	76.175	0.0	0.0	0.0
28	0.0	76.625	0.0	0.0	0.0
29	0.0	77.325	0.0	0.0	0.0
30	0.0	77.8	0.0	0.0	0.0
31	0.0	78.725	0.0	0.0	0.0
32	0.0	80.15	0.0	0.0	0.0
33	0.0	81.4	0.0	0.0	0.0
34	0.0	83.85	0.0	0.0	0.0
35	0.0	85.2	0.0	0.0	0.0
36	0.0	86.175	0.0	0.0	0.0
37	0.0	87.375	0.0	0.0	0.0
38	0.0	87.75	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	40	5.315087E-9	44.000004	8
GGTGAGT	45	2.728484E-10	44.000004	17
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
ACGCTGT	40	5.315087E-9	44.000004	9
AATCCAG	20	6.952051E-4	44.000004	8
TAAACAT	20	6.952051E-4	44.000004	3
ATCCAGG	20	6.952051E-4	44.000004	9
TTCAAGT	20	6.952051E-4	44.000004	1
TTCAACG	40	5.315087E-9	44.000004	5
AGGATAG	20	6.952051E-4	44.000004	13
TCGGTGA	40	5.315087E-9	44.000004	15
CATTCAA	40	5.315087E-9	44.000004	3
TCAACGC	40	5.315087E-9	44.000004	6
TCCAGGA	20	6.952051E-4	44.000004	10
ACATTCA	40	5.315087E-9	44.000004	2
GGATAGG	20	6.952051E-4	44.000004	14
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGTCG	40	5.315087E-9	44.000004	11
AGTAATC	20	6.952051E-4	44.000004	5
>>END_MODULE
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
Read 163312 spots for SRR5031089.sra
Written 163312 spots for SRR5031089.sra
Read 163309 spots for SRR5031089.sra
Written 163309 spots for SRR5031089.sra
SRR ids: ['SRR5031089.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6t7vnqn1
SRR5031089.sra spots: 1959711
blocks: [[1, 163309], [163310, 326618], [326619, 489927], [489928, 653236], [653237, 816545], [816546, 979854], [979855, 1143163], [1143164, 1306472], [1306473, 1469781], [1469782, 1633090], [1633091, 1796399], [1796400, 1959711]]
SRR5031089 file size 338608
SRR5031089 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031089 SRR5031089_1.fastq
Input file:	SRR5031089_1.fastq
trimmed:	SRR5031089-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:21:47 2020 >> started

Wed Mar  4 07:21:48 2020 >> done (1.287s)
1959711 reads processed; of these:
    170 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
1959537 (99.99%) reads available; of these:
 163890 ( 8.36%) trimmed reads available after processing
1795647 (91.64%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     57	  0.00%
 19	     98	  0.01%
 20	    159	  0.01%
 21	    240	  0.01%
 22	    342	  0.02%
 23	    428	  0.02%
 24	    432	  0.02%
 25	    582	  0.03%
 26	    411	  0.02%
 27	    529	  0.03%
 28	    577	  0.03%
 29	    956	  0.05%
 30	    620	  0.03%
 31	    690	  0.04%
 32	    907	  0.05%
 33	   1507	  0.08%
 34	   1871	  0.10%
 35	   4604	  0.23%
 36	   2773	  0.14%
 37	   2851	  0.15%
 38	   2701	  0.14%
 39	   3315	  0.17%
 40	   3913	  0.20%
 41	   4079	  0.21%
 42	   4786	  0.24%
 43	   7544	  0.38%
 44	   8302	  0.42%
 45	  11651	  0.59%
 46	  14051	  0.72%
 47	  13655	  0.70%
 48	  33116	  1.69%
 49	  36143	  1.84%
 50	1795647	 91.64%
1959537 reads passed initial QC


criterion=sequence-density
sequence-density=89.22
sequence-density-rank=1
fanout-score=19.09
fanout-score-rank=2
prefix-density=89.50
prefix-fanout=19.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC


criterion=fanout-score
sequence-density=3.41
sequence-density-rank=3
fanout-score=85.07
fanout-score-rank=1
prefix-density=91.22
prefix-fanout=3.2
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC -o SRR5031089 -
Input file:	STDIN
trimmed:	SRR5031089-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:01 2020 >> started

Wed Mar  4 07:22:04 2020 >> done (2.930s)
1915992 reads processed; of these:
 223195 (11.65%) short reads filtered out after trimming by size control
  24608 ( 1.28%) empty reads filtered out after trimming by size control
1668189 (87.07%) reads available; of these:
1605917 (96.27%) trimmed reads available after processing
  62272 ( 3.73%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  14200	  0.85%
 19	  29077	  1.74%
 20	 118015	  7.07%
 21	 197286	 11.83%
 22	 530313	 31.79%
 23	 250090	 14.99%
 24	 121552	  7.29%
 25	  32246	  1.93%
 26	  18230	  1.09%
 27	  14803	  0.89%
 28	  15216	  0.91%
 29	  11449	  0.69%
 30	  17273	  1.04%
 31	  24913	  1.49%
 32	  25411	  1.52%
 33	  47200	  2.83%
 34	  25058	  1.50%
 35	  19350	  1.16%
 36	  20000	  1.20%
 37	   8267	  0.50%
 38	   8459	  0.51%
 39	   6733	  0.40%
 40	   7574	  0.45%
 41	   9382	  0.56%
 42	   9110	  0.55%
 43	   6409	  0.38%
 44	   5223	  0.31%
 45	  10227	  0.61%
 46	   8006	  0.48%
 47	   5196	  0.31%
 48	    756	  0.05%
 49	    785	  0.05%
 50	  50380	  3.02%


criterion=sequence-density
sequence-density=10.86
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.36
sequence-density-rank=4
fanout-score=19.13
fanout-score-rank=1
prefix-density=2.39
prefix-fanout=18.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC
                                 Started job on |	Mar 04 07:22:24
                             Started mapping on |	Mar 04 07:22:25
                                    Finished on |	Mar 04 07:22:50
       Mapping speed, Million of reads per hour |	246.49

                          Number of input reads |	1711734
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	865305
                        Uniquely mapped reads % |	50.55%
                          Average mapped length |	23.70
                       Number of splices: Total |	2547
            Number of splices: Annotated (sjdb) |	414
                       Number of splices: GT/AG |	2459
                       Number of splices: GC/AG |	47
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	38
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.90
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	633529
             % of reads mapped to multiple loci |	37.01%
        Number of reads mapped to too many loci |	49722
             % of reads mapped to too many loci |	2.90%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.98%
                     % of reads unmapped: other |	1.55%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	212900	212900	212900
N_multimapping	633529	633529	633529
N_noFeature	50875	59359	580602
N_ambiguous	472774	217509	5330
UnstrandedReadsAssigned:341656 PositiveStrandReadsAssigned:588437 NegativeStrandReadsAssigned:279373
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031089 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031089-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,711,734 reads, 406,131 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,111 rounds

 109283 SRR5031089.ke.tsv
  52636 SRR5031089.se.tsv
 161919 total
==> SRR5031089.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031089.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031089 completed mapping pipeline successfully
