Starting /dee2/code/volunteer_pipeline.sh SRR5031068
    current disk space = 160509247488
    free memory = 73948844 
SRR5031068 SRAfilesize
ee399407c69d177137e142dc4c9d0e2a  SRR5031068.sra
SRR5031068.sra file validated
SRR5031068 is single end
SRR5031068 is conventional basespace
SRR5031068 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031068_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3165	33.0	31.0	34.0	31.0	34.0
2	32.5795	34.0	31.0	34.0	31.0	34.0
3	32.58925	34.0	31.0	34.0	31.0	34.0
4	36.196	37.0	35.0	37.0	35.0	37.0
5	35.936	37.0	35.0	37.0	35.0	37.0
6	35.76125	37.0	35.0	37.0	33.0	37.0
7	35.91	37.0	35.0	37.0	35.0	37.0
8	35.802	37.0	35.0	37.0	35.0	37.0
9	37.621	39.0	37.0	39.0	35.0	39.0
10	37.76275	39.0	37.0	39.0	35.0	39.0
11	37.587	39.0	37.0	39.0	35.0	39.0
12	37.6715	39.0	37.0	39.0	35.0	39.0
13	37.58075	39.0	37.0	39.0	35.0	39.0
14	39.06175	40.0	39.0	41.0	36.0	41.0
15	38.816	40.0	38.0	41.0	35.0	41.0
16	38.928	40.0	38.0	41.0	35.0	41.0
17	38.84675	40.0	38.0	41.0	35.0	41.0
18	39.014	40.0	38.0	41.0	35.0	41.0
19	38.70175	40.0	38.0	41.0	34.0	41.0
20	38.859	40.0	38.0	41.0	35.0	41.0
21	38.85325	40.0	38.0	41.0	35.0	41.0
22	38.99675	40.0	39.0	41.0	35.0	41.0
23	38.69475	40.0	38.0	41.0	34.0	41.0
24	38.60475	40.0	38.0	41.0	34.0	41.0
25	38.93725	40.0	38.0	41.0	35.0	41.0
26	38.74475	40.0	38.0	41.0	34.0	41.0
27	38.74	40.0	38.0	41.0	35.0	41.0
28	38.70525	40.0	38.0	41.0	35.0	41.0
29	38.38325	40.0	38.0	41.0	33.0	41.0
30	38.29775	40.0	38.0	41.0	33.0	41.0
31	38.4655	40.0	38.0	41.0	34.0	41.0
32	38.2185	40.0	38.0	41.0	33.0	41.0
33	38.2195	40.0	38.0	41.0	34.0	41.0
34	38.295	40.0	38.0	41.0	34.0	41.0
35	38.13425	40.0	38.0	41.0	34.0	41.0
36	37.937	40.0	38.0	41.0	33.0	41.0
37	37.47875	40.0	37.0	41.0	31.0	41.0
38	37.023	39.0	36.0	41.0	30.0	41.0
39	37.43325	39.0	37.0	41.0	32.0	41.0
40	37.67825	40.0	37.0	41.0	33.0	41.0
41	37.7615	40.0	37.0	41.0	33.0	41.0
42	38.02225	40.0	38.0	41.0	33.0	41.0
43	38.24125	40.0	38.0	41.0	34.0	41.0
44	37.946	40.0	38.0	41.0	33.0	41.0
45	37.92225	40.0	37.0	41.0	33.0	41.0
46	37.935	40.0	37.0	41.0	33.0	41.0
47	37.7405	40.0	37.0	41.0	33.0	41.0
48	37.3365	40.0	37.0	41.0	31.0	41.0
49	37.56475	40.0	37.0	41.0	33.0	41.0
50	37.086	40.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	0.0
20	0.0
21	2.0
22	4.0
23	9.0
24	2.0
25	7.0
26	17.0
27	23.0
28	24.0
29	44.0
30	44.0
31	58.0
32	87.0
33	119.0
34	152.0
35	201.0
36	262.0
37	399.0
38	722.0
39	1822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.9	25.0	53.449999999999996	13.65
2	24.843476083145504	27.44803405960431	19.00826446280992	28.70022539444027
3	22.025	24.224999999999998	20.45	33.300000000000004
4	32.65	28.499999999999996	14.45	24.4
5	22.975	38.324999999999996	27.725	10.975
6	34.125	15.9	35.675000000000004	14.299999999999999
7	15.1	23.325000000000003	22.875	38.7
8	26.674999999999997	29.049999999999997	15.8	28.475
9	19.375	36.725	28.075	15.825
10	20.9	25.324999999999996	26.575	27.200000000000003
11	34.275	9.2	16.8	39.725
12	15.7	20.424999999999997	28.4	35.475
13	29.225	17.275	35.55	17.95
14	33.875	28.499999999999996	23.25	14.374999999999998
15	36.95	21.65	27.725	13.675
16	18.525	18.05	25.025	38.4
17	25.224999999999998	24.325	37.65	12.8
18	26.325	16.950000000000003	28.575	28.15
19	33.35	14.774999999999999	35.225	16.650000000000002
20	46.325	13.200000000000001	27.800000000000004	12.675
21	21.025	20.724999999999998	31.474999999999998	26.775
22	29.65	11.0	50.224999999999994	9.125
23	27.500000000000004	11.375	47.8	13.325000000000001
24	54.275	12.85	25.724999999999998	7.1499999999999995
25	53.900000000000006	25.1	13.100000000000001	7.9
26	28.299999999999997	49.45	13.4	8.85
27	13.225000000000001	50.449999999999996	25.874999999999996	10.45
28	9.625	23.35	52.925	14.099999999999998
29	10.65	10.4	59.150000000000006	19.8
30	8.35	5.1499999999999995	38.425	48.075
31	13.775	3.9	45.725	36.6
32	28.875	4.45	23.05	43.625
33	58.5	6.5	11.875	23.125
34	67.5	5.4	14.825	12.275
35	66.8	5.775	19.35	8.075000000000001
36	43.5	6.5	37.8	12.2
37	44.5	9.55	20.75	25.2
38	24.45	14.524999999999999	10.725	50.3
39	12.9	24.875	9.25	52.97500000000001
40	14.524999999999999	50.64999999999999	8.9	25.924999999999997
41	26.05	51.625	7.199999999999999	15.125
42	52.325	30.099999999999998	8.924999999999999	8.649999999999999
43	54.6	29.65	6.45	9.3
44	30.85	50.724999999999994	6.525	11.899999999999999
45	15.925	52.625	11.55	19.900000000000002
46	11.525	25.95	18.15	44.375
47	7.225	12.325	39.375	41.075
48	8.575000000000001	12.4	20.775	58.25
49	11.0	20.125	11.75	57.125
50	18.975	37.974999999999994	12.174999999999999	30.875000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	1.5
30	2.0
31	3.0
32	4.0
33	5.5
34	7.0
35	7.0
36	7.0
37	13.5
38	20.0
39	23.0
40	26.0
41	31.5
42	37.0
43	98.0
44	159.0
45	183.5
46	208.0
47	351.0
48	494.0
49	589.0
50	684.0
51	822.5
52	961.0
53	674.0
54	387.0
55	415.0
56	443.0
57	326.5
58	210.0
59	149.5
60	89.0
61	67.5
62	46.0
63	74.0
64	102.0
65	61.0
66	20.0
67	14.5
68	9.0
69	14.5
70	20.0
71	37.5
72	55.0
73	29.5
74	4.0
75	2.5
76	1.0
77	1.5
78	2.0
79	1.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96443812233285	26.35
2	9.88620199146515	6.950000000000001
3	3.698435277382646	3.9
4	2.275960170697013	3.2
5	2.1337126600284493	3.75
6	1.209103840682788	2.55
7	0.7823613086770981	1.925
8	0.35561877667140823	1.0
9	0.6401137980085349	2.025
>10	3.2716927453769555	20.75
>50	0.42674253200568996	11.725
>100	0.35561877667140823	15.875
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	144	3.5999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	138	3.45	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	102	2.55	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	97	2.4250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	90	2.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	85	2.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	62	1.55	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	43	1.075	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	28	0.7000000000000001	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	26	0.65	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	19	0.475	RNA PCR Primer, Index 1 (97% over 35bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	16	0.4	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	14	0.35000000000000003	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	13	0.325	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	10	0.25	No Hit
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 40 (100% over 50bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	7	0.17500000000000002	No Hit
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC	6	0.15	RNA PCR Primer, Index 1 (97% over 36bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	6	0.15	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	6	0.15	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTC	6	0.15	RNA PCR Primer, Index 40 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATGCAAGGGCAAGCTCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	5	0.125	No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	5	0.125	No Hit
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.25	0.0	0.0	0.0
3	0.0	0.25	0.0	0.0	0.0
4	0.0	0.25	0.0	0.0	0.0
5	0.0	0.3	0.0	0.0	0.0
6	0.0	0.3	0.0	0.0	0.0
7	0.0	0.55	0.0	0.0	0.0
8	0.0	0.725	0.0	0.0	0.0
9	0.0	0.75	0.0	0.0	0.0
10	0.0	0.875	0.0	0.0	0.0
11	0.0	1.0	0.0	0.0	0.0
12	0.0	1.175	0.0	0.0	0.0
13	0.0	1.325	0.0	0.0	0.0
14	0.0	1.65	0.0	0.0	0.0
15	0.0	2.3	0.0	0.0	0.0
16	0.0	3.15	0.0	0.0	0.0
17	0.0	3.75	0.0	0.0	0.0
18	0.0	4.575	0.0	0.0	0.0
19	0.0	5.175	0.0	0.0	0.0
20	0.0	6.075	0.0	0.0	0.0
21	0.0	12.05	0.0	0.0	0.0
22	0.0	25.425	0.0	0.0	0.0
23	0.0	57.325	0.0	0.0	0.0
24	0.0	72.375	0.0	0.0	0.0
25	0.0	77.45	0.0	0.0	0.0
26	0.0	78.975	0.0	0.0	0.0
27	0.0	79.6	0.0	0.0	0.0
28	0.0	80.3	0.0	0.0	0.0
29	0.0	80.625	0.0	0.0	0.0
30	0.0	81.325	0.0	0.0	0.0
31	0.0	82.175	0.0	0.0	0.0
32	0.0	83.4	0.0	0.0	0.0
33	0.0	84.325	0.0	0.0	0.0
34	0.0	87.325	0.0	0.0	0.0
35	0.0	88.125	0.0	0.0	0.0
36	0.0	88.85	0.0	0.0	0.0
37	0.0	89.55	0.0	0.0	0.0
38	0.0	89.825	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGAGT	45	2.728484E-10	44.000004	17
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AATCCAG	20	6.952051E-4	44.000004	8
ATCCAGG	20	6.952051E-4	44.000004	9
TTCAAGT	20	6.952051E-4	44.000004	1
TTCAACG	45	2.728484E-10	44.000004	5
AGGATAG	20	6.952051E-4	44.000004	13
ATAGGCT	20	6.952051E-4	44.000004	16
AGTGGAA	20	6.952051E-4	44.000004	21
CATTCAA	45	2.728484E-10	44.000004	3
TCAACGC	45	2.728484E-10	44.000004	6
TCCAGGA	20	6.952051E-4	44.000004	10
ACATTCA	45	2.728484E-10	44.000004	2
GGATAGG	20	6.952051E-4	44.000004	14
AGTAATC	20	6.952051E-4	44.000004	5
AGGCTTG	20	6.952051E-4	44.000004	18
CGGTGAG	45	2.728484E-10	44.000004	16
TAATCCA	20	6.952051E-4	44.000004	7
CAGGATA	20	6.952051E-4	44.000004	12
>>END_MODULE
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211353 spots for SRR5031068.sra
Written 211353 spots for SRR5031068.sra
Read 211356 spots for SRR5031068.sra
Written 211356 spots for SRR5031068.sra
SRR ids: ['SRR5031068.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fsyxg4wi
SRR5031068.sra spots: 2536239
blocks: [[1, 211353], [211354, 422706], [422707, 634059], [634060, 845412], [845413, 1056765], [1056766, 1268118], [1268119, 1479471], [1479472, 1690824], [1690825, 1902177], [1902178, 2113530], [2113531, 2324883], [2324884, 2536239]]
SRR5031068 file size 438518
SRR5031068 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031068 SRR5031068_1.fastq
Input file:	SRR5031068_1.fastq
trimmed:	SRR5031068-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:33 2020 >> started

Wed Mar  4 07:17:35 2020 >> done (1.686s)
2536239 reads processed; of these:
    261 ( 0.01%) short reads filtered out after trimming by size control
      9 ( 0.00%) empty reads filtered out after trimming by size control
2535969 (99.99%) reads available; of these:
 115925 ( 4.57%) trimmed reads available after processing
2420044 (95.43%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     67	  0.00%
 19	     76	  0.00%
 20	    172	  0.01%
 21	    242	  0.01%
 22	    364	  0.01%
 23	    462	  0.02%
 24	    448	  0.02%
 25	    555	  0.02%
 26	    456	  0.02%
 27	    624	  0.02%
 28	    713	  0.03%
 29	   1207	  0.05%
 30	    761	  0.03%
 31	    841	  0.03%
 32	    876	  0.03%
 33	   1056	  0.04%
 34	   1243	  0.05%
 35	   2135	  0.08%
 36	   2050	  0.08%
 37	   2382	  0.09%
 38	   2233	  0.09%
 39	   2350	  0.09%
 40	   2600	  0.10%
 41	   2756	  0.11%
 42	   3457	  0.14%
 43	   5179	  0.20%
 44	   6616	  0.26%
 45	   7506	  0.30%
 46	  10852	  0.43%
 47	  13835	  0.55%
 48	  21047	  0.83%
 49	  20764	  0.82%
 50	2420044	 95.43%
2535969 reads passed initial QC


criterion=sequence-density
sequence-density=88.52
sequence-density-rank=1
fanout-score=22.17
fanout-score-rank=1
prefix-density=90.41
prefix-fanout=21.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC


criterion=fanout-score
sequence-density=88.52
sequence-density-rank=1
fanout-score=22.17
fanout-score-rank=1
prefix-density=90.41
prefix-fanout=21.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC -o SRR5031068 -
Input file:	STDIN
trimmed:	SRR5031068-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:52 2020 >> started

Wed Mar  4 07:17:55 2020 >> done (3.321s)
2478981 reads processed; of these:
 107809 ( 4.35%) short reads filtered out after trimming by size control
  10510 ( 0.42%) empty reads filtered out after trimming by size control
2360662 (95.23%) reads available; of these:
2265136 (95.95%) trimmed reads available after processing
  95526 ( 4.05%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12149	  0.51%
 19	  25872	  1.10%
 20	 130256	  5.52%
 21	 318141	 13.48%
 22	 779174	 33.01%
 23	 361123	 15.30%
 24	 150348	  6.37%
 25	  38207	  1.62%
 26	  19988	  0.85%
 27	  16952	  0.72%
 28	  18027	  0.76%
 29	  16488	  0.70%
 30	  24539	  1.04%
 31	  34436	  1.46%
 32	  34473	  1.46%
 33	  79319	  3.36%
 34	  25637	  1.09%
 35	  17367	  0.74%
 36	  20740	  0.88%
 37	  10716	  0.45%
 38	  11976	  0.51%
 39	  10448	  0.44%
 40	  17023	  0.72%
 41	  20281	  0.86%
 42	  23013	  0.97%
 43	  15463	  0.66%
 44	  10939	  0.46%
 45	  16209	  0.69%
 46	  11333	  0.48%
 47	   8982	  0.38%
 48	    987	  0.04%
 49	   1091	  0.05%
 50	  78965	  3.35%


criterion=sequence-density
sequence-density=8.22
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=36
fanout-score=157.97
fanout-score-rank=1
prefix-density=3.45
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 07:18:19
                             Started mapping on |	Mar 04 07:18:20
                                    Finished on |	Mar 04 07:18:49
       Mapping speed, Million of reads per hour |	300.12

                          Number of input reads |	2417650
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1237272
                        Uniquely mapped reads % |	51.18%
                          Average mapped length |	24.07
                       Number of splices: Total |	1779
            Number of splices: Annotated (sjdb) |	469
                       Number of splices: GT/AG |	1694
                       Number of splices: GC/AG |	21
                       Number of splices: AT/AC |	4
               Number of splices: Non-canonical |	60
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.81
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	880241
             % of reads mapped to multiple loci |	36.41%
        Number of reads mapped to too many loci |	48596
             % of reads mapped to too many loci |	2.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.93%
                     % of reads unmapped: other |	0.48%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	300137	300137	300137
N_multimapping	880241	880241	880241
N_noFeature	64380	77715	879541
N_ambiguous	675808	365580	10040
UnstrandedReadsAssigned:497084 PositiveStrandReadsAssigned:793977 NegativeStrandReadsAssigned:347691
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031068 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031068-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,417,650 reads, 590,670 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,093 rounds

 109283 SRR5031068.ke.tsv
  52636 SRR5031068.se.tsv
 161919 total
==> SRR5031068.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031068.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031068 completed mapping pipeline successfully
