Starting /dee2/code/volunteer_pipeline.sh SRR5030886
    current disk space = 160643661824
    free memory = 73949300 
SRR5030886 SRAfilesize
b9f4a4c413b5cb3af6d945e0f8bab600  SRR5030886.sra
SRR5030886.sra file validated
SRR5030886 is single end
SRR5030886 is conventional basespace
SRR5030886 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6525	34.0	31.0	34.0	31.0	34.0
2	32.89625	34.0	31.0	34.0	31.0	34.0
3	32.93025	34.0	31.0	34.0	31.0	34.0
4	36.33475	37.0	37.0	37.0	35.0	37.0
5	36.382	37.0	37.0	37.0	35.0	37.0
6	36.27175	37.0	37.0	37.0	35.0	37.0
7	36.1665	37.0	36.0	37.0	35.0	37.0
8	36.288	37.0	37.0	37.0	35.0	37.0
9	38.00475	39.0	39.0	39.0	35.0	39.0
10	38.14375	39.0	39.0	39.0	35.0	39.0
11	38.08575	39.0	39.0	39.0	35.0	39.0
12	38.01375	39.0	38.0	39.0	35.0	39.0
13	38.003	39.0	38.0	39.0	35.0	39.0
14	39.41225	41.0	39.0	41.0	36.0	41.0
15	39.38475	41.0	39.0	41.0	36.0	41.0
16	39.438	41.0	39.0	41.0	37.0	41.0
17	39.575	41.0	40.0	41.0	37.0	41.0
18	39.52675	41.0	39.0	41.0	36.0	41.0
19	39.4175	41.0	39.0	41.0	36.0	41.0
20	39.35675	41.0	39.0	41.0	36.0	41.0
21	39.4295	41.0	39.0	41.0	36.0	41.0
22	39.40025	41.0	39.0	41.0	36.0	41.0
23	39.31	41.0	39.0	41.0	36.0	41.0
24	38.947	40.0	39.0	41.0	35.0	41.0
25	39.22975	41.0	39.0	41.0	36.0	41.0
26	39.269	41.0	39.0	41.0	36.0	41.0
27	39.32575	41.0	39.0	41.0	36.0	41.0
28	39.11825	41.0	39.0	41.0	36.0	41.0
29	38.70775	40.0	38.0	41.0	34.0	41.0
30	38.8335	40.0	39.0	41.0	35.0	41.0
31	39.11725	41.0	39.0	41.0	35.0	41.0
32	39.3385	41.0	39.0	41.0	36.0	41.0
33	38.535	40.0	38.0	41.0	34.0	41.0
34	38.32175	40.0	38.0	41.0	33.0	41.0
35	38.0245	40.0	38.0	41.0	34.0	41.0
36	33.4295	38.0	30.0	40.0	18.0	41.0
37	35.143	38.0	33.0	40.0	27.0	41.0
38	36.95525	39.0	37.0	40.0	31.0	41.0
39	36.99525	39.0	37.0	40.0	31.0	41.0
40	37.1325	39.0	37.0	40.0	31.0	41.0
41	37.149	39.0	37.0	41.0	31.0	41.0
42	37.357	40.0	37.0	41.0	32.0	41.0
43	37.01675	39.0	37.0	41.0	31.0	41.0
44	37.085	39.0	37.0	41.0	32.0	41.0
45	36.8495	39.0	37.0	41.0	32.0	41.0
46	35.1005	38.0	34.0	40.0	26.0	41.0
47	35.49875	39.0	35.0	40.0	28.0	41.0
48	35.893	39.0	36.0	40.0	29.0	41.0
49	34.634	38.0	34.0	40.0	26.0	41.0
50	29.42275	33.0	26.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	3.0
19	1.0
20	1.0
21	5.0
22	6.0
23	8.0
24	15.0
25	16.0
26	15.0
27	25.0
28	30.0
29	35.0
30	44.0
31	62.0
32	77.0
33	107.0
34	126.0
35	197.0
36	302.0
37	559.0
38	1026.0
39	1339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.103051525762881	18.70935467733867	60.055027513756876	15.132566283141571
2	17.474999999999998	23.775	18.45	40.300000000000004
3	27.025	17.125	16.85	39.0
4	28.499999999999996	25.75	12.7	33.050000000000004
5	17.025000000000002	47.725	22.900000000000002	12.35
6	39.5	14.000000000000002	32.074999999999996	14.424999999999999
7	13.5	23.25	25.124999999999996	38.125
8	25.724999999999998	30.7	13.075000000000001	30.5
9	25.275	36.075	25.575	13.075000000000001
10	16.775000000000002	20.849999999999998	32.525	29.849999999999998
11	24.5	10.45	14.274999999999999	50.775000000000006
12	13.200000000000001	15.4	30.625000000000004	40.775
13	29.549999999999997	22.475	28.425	19.55
14	28.225	36.325	20.0	15.45
15	35.099999999999994	27.474999999999998	24.325	13.100000000000001
16	16.325	28.000000000000004	16.825000000000003	38.85
17	17.75	22.275	44.775	15.2
18	16.925	17.825	34.575	30.675
19	42.325	12.325	25.424999999999997	19.925
20	44.75	10.9	32.725	11.625
21	24.5	13.100000000000001	28.775000000000002	33.625
22	25.2	12.125	54.125	8.55
23	30.625000000000004	11.625	45.324999999999996	12.425
24	57.725	14.95	20.325	7.000000000000001
25	50.64999999999999	28.075	12.85	8.425
26	21.975	53.025	14.45	10.549999999999999
27	12.025	49.5	27.0	11.475
28	8.7	21.6	54.65	15.049999999999999
29	9.525	10.65	60.02499999999999	19.8
30	7.55	5.875	35.65	50.925
31	14.975	4.25	48.675000000000004	32.1
32	31.125000000000004	5.925	18.099999999999998	44.85
33	61.675000000000004	8.025	11.675	18.625
34	65.35	5.8999999999999995	17.2	11.55
35	65.875	4.8	19.475	9.85
36	43.85	7.2749999999999995	35.075	13.8
37	47.3	9.425	17.0	26.275
38	19.425	16.1	10.35	54.125
39	13.950000000000001	27.250000000000004	9.0	49.8
40	15.174999999999999	53.65	10.075000000000001	21.099999999999998
41	27.775	50.7	7.1499999999999995	14.374999999999998
42	54.800000000000004	28.775000000000002	9.575	6.8500000000000005
43	51.300000000000004	32.6	7.199999999999999	8.9
44	25.3	55.074999999999996	6.75	12.875
45	16.675	51.525	11.5	20.3
46	10.274999999999999	23.9	20.625	45.2
47	6.425	13.750000000000002	42.15	37.675
48	10.05	13.875000000000002	17.325	58.75
49	14.2	20.075000000000003	11.475	54.25
50	20.75	38.725	12.625	27.900000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	2.5
30	5.0
31	15.0
32	25.0
33	19.5
34	14.0
35	23.0
36	32.0
37	28.5
38	25.0
39	29.5
40	34.0
41	35.0
42	36.0
43	57.0
44	78.0
45	120.0
46	162.0
47	301.5
48	441.0
49	501.0
50	561.0
51	752.0
52	943.0
53	730.5
54	518.0
55	518.5
56	519.0
57	415.5
58	312.0
59	189.5
60	67.0
61	53.5
62	40.0
63	75.0
64	110.0
65	80.0
66	50.0
67	31.5
68	13.0
69	8.5
70	4.0
71	3.0
72	2.0
73	2.5
74	3.0
75	2.0
76	1.0
77	1.0
78	1.0
79	1.5
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28248587570621	26.650000000000002
2	9.533898305084746	6.75
3	3.8135593220338984	4.05
4	2.4717514124293785	3.5000000000000004
5	1.694915254237288	3.0
6	1.4124293785310735	3.0
7	0.6355932203389831	1.575
8	0.5649717514124294	1.6
9	0.49435028248587576	1.575
>10	3.4604519774011298	24.7
>50	0.423728813559322	11.325000000000001
>100	0.211864406779661	12.275
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	178	4.45	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	168	4.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	145	3.6249999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	86	2.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	84	2.1	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	81	2.025	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	73	1.825	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	67	1.675	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	62	1.55	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	48	1.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC	31	0.775	RNA PCR Primer, Index 5 (100% over 50bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	16	0.4	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	15	0.375	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	14	0.35000000000000003	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	13	0.325	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	12	0.3	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	12	0.3	No Hit
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	10	0.25	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	10	0.25	No Hit
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	9	0.22499999999999998	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	8	0.2	RNA PCR Primer, Index 1 (96% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	8	0.2	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	7	0.17500000000000002	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	6	0.15	No Hit
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATGGAAT	6	0.15	No Hit
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.875	0.0	0.0	0.0
2	0.0	0.875	0.0	0.0	0.0
3	0.0	0.875	0.0	0.0	0.0
4	0.0	0.875	0.0	0.0	0.0
5	0.0	0.9	0.0	0.0	0.0
6	0.0	0.925	0.0	0.0	0.0
7	0.0	1.0	0.0	0.0	0.0
8	0.0	1.05	0.0	0.0	0.0
9	0.0	1.175	0.0	0.0	0.0
10	0.0	1.25	0.0	0.0	0.0
11	0.0	1.45	0.0	0.0	0.0
12	0.0	1.575	0.0	0.0	0.0
13	0.0	1.775	0.0	0.0	0.0
14	0.0	2.05	0.0	0.0	0.0
15	0.0	2.475	0.0	0.0	0.0
16	0.0	2.775	0.0	0.0	0.0
17	0.0	3.225	0.0	0.0	0.0
18	0.0	3.625	0.0	0.0	0.0
19	0.0	4.225	0.0	0.0	0.0
20	0.0	5.4	0.0	0.0	0.0
21	0.0	12.7	0.0	0.0	0.0
22	0.0	26.875	0.0	0.0	0.0
23	0.0	60.8	0.0	0.0	0.0
24	0.0	72.2	0.0	0.0	0.0
25	0.0	77.425	0.0	0.0	0.0
26	0.0	78.925	0.0	0.0	0.0
27	0.0	79.575	0.0	0.0	0.0
28	0.0	79.85	0.0	0.0	0.0
29	0.0	80.35	0.0	0.0	0.0
30	0.0	80.575	0.0	0.0	0.0
31	0.0	81.15	0.0	0.0	0.0
32	0.0	81.7	0.0	0.0	0.0
33	0.0	82.275	0.0	0.0	0.0
34	0.0	85.525	0.0	0.0	0.0
35	0.0	86.075	0.0	0.0	0.0
36	0.0	86.75	0.0	0.0	0.0
37	0.0	87.125	0.0	0.0	0.0
38	0.0	87.4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	45	2.728484E-10	44.000004	5
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
GAGGTAG	20	6.952051E-4	44.000004	2
AACCGGT	20	6.952051E-4	44.000004	9
CCTGTTG	20	6.952051E-4	44.000004	18
GGTCTCT	20	6.952051E-4	44.000004	13
ACCTGTT	20	6.952051E-4	44.000004	17
CTTGGCT	45	2.728484E-10	44.000004	16
GAACCGG	20	6.952051E-4	44.000004	8
GTCTCTT	20	6.952051E-4	44.000004	14
GGTAGTA	20	6.952051E-4	44.000004	4
GCTTGGC	45	2.728484E-10	44.000004	15
TGAGGTA	20	6.952051E-4	44.000004	1
AGTGAAC	20	6.952051E-4	44.000004	5
GTGAACC	20	6.952051E-4	44.000004	6
AGACCCT	45	2.728484E-10	44.000004	7
CGGTCTC	20	6.952051E-4	44.000004	12
TGAACCG	20	6.952051E-4	44.000004	7
CAGTGAA	20	6.952051E-4	44.000004	4
>>END_MODULE
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
Read 169209 spots for SRR5030886.sra
Written 169209 spots for SRR5030886.sra
SRR ids: ['SRR5030886.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_z3yxw6hz
SRR5030886.sra spots: 2030508
blocks: [[1, 169209], [169210, 338418], [338419, 507627], [507628, 676836], [676837, 846045], [846046, 1015254], [1015255, 1184463], [1184464, 1353672], [1353673, 1522881], [1522882, 1692090], [1692091, 1861299], [1861300, 2030508]]
SRR5030886 file size 350853
SRR5030886 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030886 SRR5030886_1.fastq
Input file:	SRR5030886_1.fastq
trimmed:	SRR5030886-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:36:56 2020 >> started

Wed Mar  4 06:36:59 2020 >> done (2.676s)
2030508 reads processed; of these:
     62 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2030446 (100.00%) reads available; of these:
 124370 ( 6.13%) trimmed reads available after processing
1906076 (93.87%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     19	  0.00%
 19	     31	  0.00%
 20	     74	  0.00%
 21	     87	  0.00%
 22	    133	  0.01%
 23	    165	  0.01%
 24	    155	  0.01%
 25	    255	  0.01%
 26	    193	  0.01%
 27	    255	  0.01%
 28	    322	  0.02%
 29	    477	  0.02%
 30	    337	  0.02%
 31	    551	  0.03%
 32	    481	  0.02%
 33	    836	  0.04%
 34	   2193	  0.11%
 35	   2043	  0.10%
 36	   1299	  0.06%
 37	   1452	  0.07%
 38	   1583	  0.08%
 39	   1699	  0.08%
 40	   1801	  0.09%
 41	   2020	  0.10%
 42	   2789	  0.14%
 43	   3652	  0.18%
 44	   7332	  0.36%
 45	   8497	  0.42%
 46	   8218	  0.40%
 47	  10946	  0.54%
 48	  26977	  1.33%
 49	  37498	  1.85%
 50	1906076	 93.87%
2030446 reads passed initial QC


criterion=sequence-density
sequence-density=87.10
sequence-density-rank=1
fanout-score=17.59
fanout-score-rank=1
prefix-density=88.12
prefix-fanout=17.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC


criterion=fanout-score
sequence-density=87.10
sequence-density-rank=1
fanout-score=17.59
fanout-score-rank=1
prefix-density=88.12
prefix-fanout=17.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC -o SRR5030886 -
Input file:	STDIN
trimmed:	SRR5030886-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:37:15 2020 >> started

Wed Mar  4 06:37:21 2020 >> done (5.506s)
1984300 reads processed; of these:
  59220 ( 2.98%) short reads filtered out after trimming by size control
  16963 ( 0.85%) empty reads filtered out after trimming by size control
1908117 (96.16%) reads available; of these:
1773914 (92.97%) trimmed reads available after processing
 134203 ( 7.03%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12972	  0.68%
 19	  19335	  1.01%
 20	 149880	  7.85%
 21	 287642	 15.07%
 22	 674863	 35.37%
 23	 227201	 11.91%
 24	 106626	  5.59%
 25	  27176	  1.42%
 26	  12328	  0.65%
 27	   8846	  0.46%
 28	   8977	  0.47%
 29	   6734	  0.35%
 30	   9313	  0.49%
 31	  13043	  0.68%
 32	  12303	  0.64%
 33	  68680	  3.60%
 34	  13016	  0.68%
 35	   9750	  0.51%
 36	   9147	  0.48%
 37	   5834	  0.31%
 38	   6323	  0.33%
 39	   7724	  0.40%
 40	  13151	  0.69%
 41	  13932	  0.73%
 42	  14782	  0.77%
 43	   9738	  0.51%
 44	   9042	  0.47%
 45	   9712	  0.51%
 46	   8053	  0.42%
 47	   6457	  0.34%
 48	   1479	  0.08%
 49	   6590	  0.35%
 50	 117468	  6.16%


criterion=sequence-density
sequence-density=4.10
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=8
prefix-density=0.02
prefix-fanout=2.0
sequence=CCTGAGACCCTAACTTGTGAAA


criterion=fanout-score
sequence-density=0.52
sequence-density-rank=20
fanout-score=27.62
fanout-score-rank=1
prefix-density=14.32
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:37:51
                             Started mapping on |	Mar 04 06:37:52
                                    Finished on |	Mar 04 06:38:17
       Mapping speed, Million of reads per hour |	281.41

                          Number of input reads |	1954263
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1201997
                        Uniquely mapped reads % |	61.51%
                          Average mapped length |	25.20
                       Number of splices: Total |	638
            Number of splices: Annotated (sjdb) |	102
                       Number of splices: GT/AG |	605
                       Number of splices: GC/AG |	6
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	27
                      Mismatch rate per base, % |	0.14%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.20
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	559160
             % of reads mapped to multiple loci |	28.61%
        Number of reads mapped to too many loci |	25447
             % of reads mapped to too many loci |	1.30%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.34%
                     % of reads unmapped: other |	0.24%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	193106	193106	193106
N_multimapping	559160	559160	559160
N_noFeature	32935	41544	793939
N_ambiguous	722408	374120	6737
UnstrandedReadsAssigned:446654 PositiveStrandReadsAssigned:786333 NegativeStrandReadsAssigned:401321
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030886 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030886-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,954,263 reads, 609,809 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,069 rounds

 109283 SRR5030886.ke.tsv
  52636 SRR5030886.se.tsv
 161919 total
==> SRR5030886.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030886.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030886 completed mapping pipeline successfully
