Starting /dee2/code/volunteer_pipeline.sh SRR5030920
    current disk space = 160641105920
    free memory = 73925468 
SRR5030920 SRAfilesize
497267b8165201012d0f752daa96b75e  SRR5030920.sra
SRR5030920.sra file validated
SRR5030920 is single end
SRR5030920 is conventional basespace
SRR5030920 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030920_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4065	33.0	31.0	34.0	31.0	34.0
2	32.7355	34.0	31.0	34.0	31.0	34.0
3	32.64075	34.0	31.0	34.0	31.0	34.0
4	36.258	37.0	37.0	37.0	35.0	37.0
5	36.3395	37.0	37.0	37.0	35.0	37.0
6	36.15775	37.0	36.0	37.0	35.0	37.0
7	36.12025	37.0	35.0	37.0	35.0	37.0
8	36.22075	37.0	36.0	37.0	35.0	37.0
9	37.898	39.0	38.0	39.0	35.0	39.0
10	37.87875	39.0	38.0	39.0	35.0	39.0
11	37.92625	39.0	38.0	39.0	35.0	39.0
12	37.6965	39.0	38.0	39.0	35.0	39.0
13	37.6935	39.0	38.0	39.0	35.0	39.0
14	39.24175	41.0	39.0	41.0	36.0	41.0
15	39.313	41.0	39.0	41.0	36.0	41.0
16	39.17175	40.0	38.0	41.0	36.0	41.0
17	39.25825	40.0	39.0	41.0	36.0	41.0
18	39.407	41.0	39.0	41.0	36.0	41.0
19	39.3665	41.0	39.0	41.0	36.0	41.0
20	39.19	41.0	39.0	41.0	36.0	41.0
21	39.1685	41.0	39.0	41.0	36.0	41.0
22	39.30875	41.0	39.0	41.0	36.0	41.0
23	39.2995	41.0	39.0	41.0	36.0	41.0
24	38.63225	40.0	38.0	41.0	34.0	41.0
25	38.69475	40.0	39.0	41.0	34.0	41.0
26	39.0255	41.0	39.0	41.0	35.0	41.0
27	39.1045	41.0	39.0	41.0	36.0	41.0
28	38.9835	40.0	39.0	41.0	35.0	41.0
29	38.5245	40.0	38.0	41.0	33.0	41.0
30	38.68225	40.0	38.0	41.0	34.0	41.0
31	38.98125	40.0	39.0	41.0	35.0	41.0
32	38.9165	40.0	39.0	41.0	35.0	41.0
33	37.9405	40.0	38.0	41.0	33.0	41.0
34	37.688	40.0	38.0	41.0	31.0	41.0
35	37.425	40.0	37.0	41.0	31.0	41.0
36	32.55525	37.0	29.0	40.0	16.0	41.0
37	35.14825	38.0	33.0	40.0	28.0	41.0
38	36.97225	39.0	37.0	40.0	31.0	41.0
39	37.22875	39.0	37.0	40.0	32.0	41.0
40	37.257	39.0	37.0	40.0	32.0	41.0
41	36.95575	39.0	36.0	40.0	31.0	41.0
42	37.10425	39.0	37.0	40.0	31.0	41.0
43	37.02475	39.0	36.0	41.0	31.0	41.0
44	37.33575	40.0	37.0	41.0	32.0	41.0
45	36.78875	39.0	36.0	41.0	31.0	41.0
46	34.5605	38.0	33.0	40.0	24.0	41.0
47	35.685	39.0	35.0	40.0	28.0	41.0
48	35.83475	39.0	35.0	40.0	30.0	41.0
49	35.066	39.0	35.0	40.0	28.0	41.0
50	30.72425	34.0	28.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	0.0
17	0.0
18	0.0
19	3.0
20	7.0
21	2.0
22	14.0
23	10.0
24	8.0
25	17.0
26	17.0
27	39.0
28	40.0
29	38.0
30	52.0
31	76.0
32	68.0
33	100.0
34	131.0
35	194.0
36	331.0
37	508.0
38	1086.0
39	1256.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.056698444555945	19.543401906673356	55.77019568489714	15.629703963873556
2	21.9	24.15	23.974999999999998	29.975
3	18.625	19.3	20.275000000000002	41.8
4	25.7	34.9	14.075	25.324999999999996
5	20.1	40.45	26.1	13.350000000000001
6	37.525	15.625	35.35	11.5
7	16.825000000000003	23.925	26.575	32.675
8	25.224999999999998	40.1	13.700000000000001	20.974999999999998
9	20.325	38.2	29.075	12.4
10	21.075	19.6	29.599999999999998	29.725
11	27.875	14.549999999999999	13.525	44.05
12	15.275	17.625	24.3	42.8
13	21.5	23.549999999999997	34.125	20.825
14	36.375	25.474999999999998	25.624999999999996	12.525
15	39.125	21.5	27.125	12.25
16	13.05	29.349999999999998	21.25	36.35
17	21.875	22.925	40.35	14.85
18	25.45	20.1	30.375000000000004	24.075
19	37.675	15.2	28.95	18.175
20	47.475	17.474999999999998	23.200000000000003	11.85
21	21.7	21.575	25.4	31.324999999999996
22	29.075	13.25	48.699999999999996	8.975
23	26.5	13.925	46.075	13.5
24	52.275000000000006	13.675	26.25	7.8
25	53.175	23.150000000000002	13.675	10.0
26	28.375	48.199999999999996	12.15	11.275
27	14.399999999999999	51.175000000000004	23.325000000000003	11.1
28	10.100000000000001	25.2	51.625	13.075000000000001
29	8.75	11.75	59.650000000000006	19.85
30	9.6	6.35	37.8	46.25
31	14.45	5.8500000000000005	45.45	34.25
32	26.424999999999997	6.875000000000001	22.7	44.0
33	55.400000000000006	8.125	12.425	24.05
34	64.875	8.1	14.05	12.975
35	63.925	7.55	18.75	9.775
36	45.1	7.8	34.050000000000004	13.05
37	44.324999999999996	9.425	22.525000000000002	23.724999999999998
38	23.25	13.575000000000001	12.125	51.05
39	14.424999999999999	24.25	8.625	52.7
40	14.224999999999998	50.5	10.6	24.675
41	25.224999999999998	52.15	9.85	12.775
42	52.949999999999996	29.799999999999997	8.975	8.275
43	53.87499999999999	28.875	8.975	8.275
44	29.675	50.875	7.675	11.774999999999999
45	14.95	54.625	11.725	18.7
46	10.299999999999999	28.1	19.975	41.625
47	8.55	13.850000000000001	40.325	37.275000000000006
48	10.125	12.049999999999999	21.7	56.125
49	12.75	21.375	13.100000000000001	52.775000000000006
50	19.525000000000002	38.175	13.3	28.999999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	1.5
26	3.0
27	2.5
28	2.0
29	1.5
30	1.0
31	5.5
32	10.0
33	18.0
34	26.0
35	38.5
36	51.0
37	56.0
38	61.0
39	63.0
40	65.0
41	61.0
42	57.0
43	94.0
44	131.0
45	187.5
46	244.0
47	405.0
48	566.0
49	570.0
50	574.0
51	733.5
52	893.0
53	651.0
54	409.0
55	372.5
56	336.0
57	316.0
58	296.0
59	181.5
60	67.0
61	49.0
62	31.0
63	50.5
64	70.0
65	44.0
66	18.0
67	14.0
68	10.0
69	16.5
70	23.0
71	28.0
72	33.0
73	22.5
74	12.0
75	8.0
76	4.0
77	3.0
78	2.0
79	1.5
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35000000000000003
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10540013012363	28.475
2	10.930383864671438	8.4
3	4.033832140533507	4.65
4	1.951854261548471	3.0
5	1.1711125569290826	2.25
6	1.3012361743656473	3.0
7	0.7807417046193884	2.1
8	1.040988939492518	3.2
9	0.2602472348731295	0.8999999999999999
>10	4.033832140533507	27.85
>50	0.1951854261548471	4.65
>100	0.1951854261548471	11.525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	212	5.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	131	3.2750000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	78	1.95	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	55	1.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	53	1.325	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	45	1.125	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	27	0.675	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	21	0.525	No Hit
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	19	0.475	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	16	0.4	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	14	0.35000000000000003	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 35bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC	12	0.3	RNA PCR Primer, Index 15 (100% over 44bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	12	0.3	RNA PCR Primer, Index 1 (100% over 33bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	11	0.27499999999999997	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	10	0.25	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	10	0.25	No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	9	0.22499999999999998	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	8	0.2	RNA PCR Primer, Index 1 (100% over 34bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	8	0.2	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC	8	0.2	RNA PCR Primer, Index 15 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	6	0.15	No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	6	0.15	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	6	0.15	No Hit
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTGTGGAAT	5	0.125	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCATTGTTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	5	0.125	No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGTACTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTG	5	0.125	No Hit
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.2	0.0	0.0	0.0
2	0.0	0.225	0.0	0.0	0.0
3	0.0	0.3	0.0	0.0	0.0
4	0.0	0.4	0.0	0.0	0.0
5	0.0	0.45	0.0	0.0	0.0
6	0.0	0.525	0.0	0.0	0.0
7	0.0	0.875	0.0	0.0	0.0
8	0.0	0.975	0.0	0.0	0.0
9	0.0	0.975	0.0	0.0	0.0
10	0.0	1.1	0.0	0.0	0.0
11	0.0	1.275	0.0	0.0	0.0
12	0.0	1.525	0.0	0.0	0.0
13	0.0	1.825	0.0	0.0	0.0
14	0.0	1.975	0.0	0.0	0.0
15	0.0	2.2	0.0	0.0	0.0
16	0.0	3.025	0.0	0.0	0.0
17	0.0	3.625	0.0	0.0	0.0
18	0.0	4.3	0.0	0.0	0.0
19	0.0	4.75	0.0	0.0	0.0
20	0.0	5.75	0.0	0.0	0.0
21	0.0	10.35	0.0	0.0	0.0
22	0.0	22.225	0.0	0.0	0.0
23	0.0	53.675	0.0	0.0	0.0
24	0.0	67.9	0.0	0.0	0.0
25	0.0	72.575	0.0	0.0	0.0
26	0.0	73.475	0.0	0.0	0.0
27	0.0	73.925	0.0	0.0	0.0
28	0.0	74.375	0.0	0.0	0.0
29	0.0	74.825	0.0	0.0	0.0
30	0.0	75.075	0.0	0.0	0.0
31	0.0	75.65	0.0	0.0	0.0
32	0.0	76.5	0.0	0.0	0.0
33	0.0	77.1	0.0	0.0	0.0
34	0.0	78.5	0.0	0.0	0.0
35	0.0	79.4	0.0	0.0	0.0
36	0.0	79.9	0.0	0.0	0.0
37	0.0	80.6	0.0	0.0	0.0
38	0.0	81.05	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	40	5.315087E-9	44.000004	8
GGTGAGT	40	5.315087E-9	44.000004	17
ACGCTGT	40	5.315087E-9	44.000004	9
TCTAGCT	20	6.952051E-4	44.000004	11
TTCAACG	40	5.315087E-9	44.000004	5
TTATCTA	20	6.952051E-4	44.000004	8
TCGGTGA	40	5.315087E-9	44.000004	15
CATTCAA	40	5.315087E-9	44.000004	3
TCAACGC	40	5.315087E-9	44.000004	6
ACATTCA	40	5.315087E-9	44.000004	2
GCTGTCG	40	5.315087E-9	44.000004	11
GCTGTAT	20	6.952051E-4	44.000004	15
TATCTAG	20	6.952051E-4	44.000004	9
CGGTGAG	40	5.315087E-9	44.000004	16
CTAGCTG	20	6.952051E-4	44.000004	12
GCTTGGT	20	6.952051E-4	44.000004	15
TTTGGTT	20	6.952051E-4	44.000004	3
TTGGTTA	20	6.952051E-4	44.000004	4
TTGGTGG	20	6.952051E-4	44.000004	17
CTTTGGT	20	6.952051E-4	44.000004	2
>>END_MODULE
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355687 spots for SRR5030920.sra
Written 355687 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
Read 355677 spots for SRR5030920.sra
Written 355677 spots for SRR5030920.sra
SRR ids: ['SRR5030920.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qnz4vyk2
SRR5030920.sra spots: 4268134
blocks: [[1, 355677], [355678, 711354], [711355, 1067031], [1067032, 1422708], [1422709, 1778385], [1778386, 2134062], [2134063, 2489739], [2489740, 2845416], [2845417, 3201093], [3201094, 3556770], [3556771, 3912447], [3912448, 4268134]]
SRR5030920 file size 738684
SRR5030920 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030920 SRR5030920_1.fastq
Input file:	SRR5030920_1.fastq
trimmed:	SRR5030920-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:45:05 2020 >> started

Wed Mar  4 06:45:08 2020 >> done (2.467s)
4268134 reads processed; of these:
    161 ( 0.00%) short reads filtered out after trimming by size control
     12 ( 0.00%) empty reads filtered out after trimming by size control
4267961 (100.00%) reads available; of these:
 319291 ( 7.48%) trimmed reads available after processing
3948670 (92.52%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     40	  0.00%
 19	     75	  0.00%
 20	    189	  0.00%
 21	    218	  0.01%
 22	    360	  0.01%
 23	    429	  0.01%
 24	    403	  0.01%
 25	    521	  0.01%
 26	    469	  0.01%
 27	    699	  0.02%
 28	    904	  0.02%
 29	   1486	  0.03%
 30	   1021	  0.02%
 31	   1336	  0.03%
 32	   1819	  0.04%
 33	   3134	  0.07%
 34	  11539	  0.27%
 35	   5474	  0.13%
 36	   3982	  0.09%
 37	   3610	  0.08%
 38	   5775	  0.14%
 39	   4938	  0.12%
 40	   8792	  0.21%
 41	   6227	  0.15%
 42	  11559	  0.27%
 43	   8164	  0.19%
 44	  24390	  0.57%
 45	  21309	  0.50%
 46	  18634	  0.44%
 47	  22905	  0.54%
 48	  61772	  1.45%
 49	  87118	  2.04%
 50	3948670	 92.52%
4267961 reads passed initial QC


criterion=sequence-density
sequence-density=80.58
sequence-density-rank=1
fanout-score=18.61
fanout-score-rank=2
prefix-density=82.76
prefix-fanout=18.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC


criterion=fanout-score
sequence-density=2.38
sequence-density-rank=5
fanout-score=102.01
fanout-score-rank=1
prefix-density=82.41
prefix-fanout=2.9
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC -o SRR5030920 -
Input file:	STDIN
trimmed:	SRR5030920-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:45:26 2020 >> started

Wed Mar  4 06:45:31 2020 >> done (5.189s)
4162579 reads processed; of these:
 162280 ( 3.90%) short reads filtered out after trimming by size control
  11254 ( 0.27%) empty reads filtered out after trimming by size control
3989045 (95.83%) reads available; of these:
3538834 (88.71%) trimmed reads available after processing
 450211 (11.29%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  22740	  0.57%
 19	  43131	  1.08%
 20	 189812	  4.76%
 21	 438823	 11.00%
 22	1321979	 33.14%
 23	 606231	 15.20%
 24	 221232	  5.55%
 25	  38858	  0.97%
 26	  19168	  0.48%
 27	  18174	  0.46%
 28	  20633	  0.52%
 29	  16649	  0.42%
 30	  30095	  0.75%
 31	  36665	  0.92%
 32	  43945	  1.10%
 33	  73017	  1.83%
 34	  38850	  0.97%
 35	  26657	  0.67%
 36	  31267	  0.78%
 37	  19752	  0.50%
 38	  20800	  0.52%
 39	  22243	  0.56%
 40	  30419	  0.76%
 41	  40558	  1.02%
 42	  45358	  1.14%
 43	  34922	  0.88%
 44	  31618	  0.79%
 45	  35701	  0.89%
 46	  29047	  0.73%
 47	  23217	  0.58%
 48	   5456	  0.14%
 49	  16525	  0.41%
 50	 395503	  9.91%


criterion=sequence-density
sequence-density=8.16
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=33
fanout-score=16.96
fanout-score-rank=1
prefix-density=7.02
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:45:53
                             Started mapping on |	Mar 04 06:45:53
                                    Finished on |	Mar 04 06:46:23
       Mapping speed, Million of reads per hour |	491.33

                          Number of input reads |	4094427
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2162141
                        Uniquely mapped reads % |	52.81%
                          Average mapped length |	27.55
                       Number of splices: Total |	1787
            Number of splices: Annotated (sjdb) |	275
                       Number of splices: GT/AG |	1580
                       Number of splices: GC/AG |	24
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	181
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.49
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1507247
             % of reads mapped to multiple loci |	36.81%
        Number of reads mapped to too many loci |	95108
             % of reads mapped to too many loci |	2.32%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.40%
                     % of reads unmapped: other |	0.66%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	425039	425039	425039
N_multimapping	1507247	1507247	1507247
N_noFeature	92655	114439	1663363
N_ambiguous	1170049	746155	21066
UnstrandedReadsAssigned:899437 PositiveStrandReadsAssigned:1301547 NegativeStrandReadsAssigned:477712
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030920 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030920-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,094,427 reads, 889,812 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,163 rounds

 109283 SRR5030920.ke.tsv
  52636 SRR5030920.se.tsv
 161919 total
==> SRR5030920.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030920.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030920 completed mapping pipeline successfully
