Starting /dee2/code/volunteer_pipeline.sh SRR5030854
    current disk space = 160654868480
    free memory = 73947884 
SRR5030854 SRAfilesize
0c2cac6b1f59581124be921a095e01a7  SRR5030854.sra
SRR5030854.sra file validated
SRR5030854 is single end
SRR5030854 is conventional basespace
SRR5030854 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3515	33.0	31.0	34.0	31.0	34.0
2	32.72825	34.0	31.0	34.0	31.0	34.0
3	32.831	34.0	31.0	34.0	31.0	34.0
4	36.23875	37.0	37.0	37.0	35.0	37.0
5	36.33075	37.0	37.0	37.0	35.0	37.0
6	36.13325	37.0	36.0	37.0	35.0	37.0
7	36.0875	37.0	35.0	37.0	35.0	37.0
8	36.14	37.0	36.0	37.0	35.0	37.0
9	37.982	39.0	38.0	39.0	35.0	39.0
10	38.01325	39.0	39.0	39.0	35.0	39.0
11	37.942	39.0	38.0	39.0	35.0	39.0
12	37.94425	39.0	38.0	39.0	35.0	39.0
13	37.84675	39.0	38.0	39.0	35.0	39.0
14	39.151	41.0	39.0	41.0	36.0	41.0
15	39.06675	40.0	38.0	41.0	36.0	41.0
16	39.16025	40.0	39.0	41.0	36.0	41.0
17	39.33775	41.0	39.0	41.0	36.0	41.0
18	39.32975	41.0	39.0	41.0	36.0	41.0
19	39.0435	40.0	39.0	41.0	35.0	41.0
20	39.0295	40.0	39.0	41.0	35.0	41.0
21	39.2	40.0	39.0	41.0	36.0	41.0
22	39.1105	41.0	39.0	41.0	36.0	41.0
23	38.9155	40.0	39.0	41.0	35.0	41.0
24	38.74775	40.0	38.0	41.0	35.0	41.0
25	39.04775	41.0	39.0	41.0	35.0	41.0
26	39.0805	41.0	39.0	41.0	35.0	41.0
27	39.08625	41.0	39.0	41.0	36.0	41.0
28	38.829	40.0	38.0	41.0	35.0	41.0
29	38.0415	40.0	38.0	41.0	32.0	41.0
30	38.15425	40.0	38.0	41.0	33.0	41.0
31	38.51875	40.0	38.0	41.0	34.0	41.0
32	38.69425	40.0	38.0	41.0	35.0	41.0
33	37.84575	40.0	38.0	41.0	32.0	41.0
34	37.9165	40.0	38.0	41.0	33.0	41.0
35	37.66175	39.0	37.0	40.0	33.0	41.0
36	34.51	38.0	33.0	40.0	23.0	41.0
37	35.60575	38.0	34.0	40.0	30.0	41.0
38	36.97675	39.0	36.0	40.0	32.0	41.0
39	37.023	39.0	37.0	40.0	31.0	41.0
40	36.9795	39.0	37.0	40.0	31.0	41.0
41	36.864	39.0	36.0	40.0	31.0	41.0
42	37.0055	40.0	36.0	41.0	31.0	41.0
43	36.90725	39.0	36.0	41.0	31.0	41.0
44	36.975	39.0	36.0	41.0	31.0	41.0
45	36.7	39.0	36.0	41.0	31.0	41.0
46	34.9355	38.0	34.0	40.0	26.0	41.0
47	35.4185	38.0	35.0	40.0	28.0	41.0
48	35.57875	38.0	35.0	40.0	29.0	41.0
49	34.72925	38.0	34.0	40.0	27.0	41.0
50	30.2895	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	3.0
16	2.0
17	0.0
18	1.0
19	1.0
20	3.0
21	6.0
22	5.0
23	8.0
24	11.0
25	26.0
26	19.0
27	32.0
28	35.0
29	34.0
30	47.0
31	52.0
32	70.0
33	126.0
34	151.0
35	220.0
36	321.0
37	574.0
38	1069.0
39	1183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.177944862155389	23.784461152882205	48.17042606516291	16.8671679197995
2	23.849999999999998	26.450000000000003	21.5	28.199999999999996
3	25.074999999999996	21.349999999999998	24.65	28.925
4	30.925000000000004	28.000000000000004	15.725	25.35
5	21.675	39.125	25.900000000000002	13.3
6	33.074999999999996	17.075000000000003	33.95	15.9
7	20.7	20.125	24.05	35.125
8	24.875	30.5	15.5	29.125
9	18.675	32.675	30.85	17.8
10	24.675	20.200000000000003	25.924999999999997	29.2
11	33.025	12.525	17.075000000000003	37.375
12	19.900000000000002	20.375	28.275	31.45
13	31.474999999999998	19.675	29.599999999999998	19.25
14	32.125	29.675	22.35	15.85
15	32.98324581145287	25.256314078519633	26.731682920730183	15.028757189297323
16	17.95	20.674999999999997	24.375	37.0
17	22.85	21.625	36.449999999999996	19.075
18	24.099999999999998	19.7	29.099999999999998	27.1
19	32.05	15.725	32.875	19.35
20	38.324999999999996	18.65	27.224999999999998	15.8
21	24.325	21.375	26.674999999999997	27.625
22	28.849999999999998	14.7	42.95	13.5
23	27.900000000000002	14.774999999999999	39.25	18.075
24	48.575	13.55	25.224999999999998	12.65
25	47.3	23.95	16.325	12.425
26	28.975	41.075	14.799999999999999	15.15
27	17.25	41.949999999999996	23.400000000000002	17.4
28	13.3	25.7	44.675	16.325
29	14.274999999999999	15.2	51.625	18.9
30	11.625	8.5	36.675000000000004	43.2
31	16.575	7.449999999999999	41.85	34.125
32	28.525	8.725	21.65	41.099999999999994
33	47.825	9.275	15.225	27.675
34	54.425000000000004	7.449999999999999	19.125	19.0
35	57.99999999999999	9.325	19.8	12.875
36	42.875	8.725	33.074999999999996	15.325
37	42.75	12.475	19.775000000000002	25.0
38	26.200000000000003	15.7	14.149999999999999	43.95
39	19.400000000000002	23.375	12.25	44.975
40	18.35	45.324999999999996	12.275	24.05
41	26.075	43.6	13.25	17.075000000000003
42	45.5	28.000000000000004	12.325	14.174999999999999
43	46.475	30.349999999999998	11.4	11.774999999999999
44	29.625	44.375	10.424999999999999	15.575
45	20.724999999999998	45.550000000000004	13.575000000000001	20.150000000000002
46	16.05	26.924999999999997	20.150000000000002	36.875
47	11.924999999999999	17.45	35.55	35.075
48	11.600000000000001	13.875000000000002	23.400000000000002	51.125
49	16.35	19.55	16.7	47.4
50	21.349999999999998	31.85	14.825	31.974999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	2.0
15	1.0
16	0.0
17	1.0
18	2.0
19	1.0
20	0.0
21	0.0
22	0.0
23	1.0
24	2.0
25	2.0
26	2.0
27	2.0
28	2.0
29	3.0
30	4.0
31	8.5
32	13.0
33	19.5
34	26.0
35	34.0
36	42.0
37	50.5
38	59.0
39	65.5
40	72.0
41	68.5
42	65.0
43	107.0
44	149.0
45	184.0
46	219.0
47	310.0
48	401.0
49	497.0
50	593.0
51	673.0
52	753.0
53	589.5
54	426.0
55	422.0
56	418.0
57	322.5
58	227.0
59	166.0
60	105.0
61	80.0
62	55.0
63	78.0
64	101.0
65	63.5
66	26.0
67	25.0
68	24.0
69	38.5
70	53.0
71	93.0
72	133.0
73	74.0
74	15.0
75	8.5
76	2.0
77	2.0
78	2.0
79	3.0
80	4.0
81	2.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.346037507562	31.55
2	9.497882637628555	7.85
3	4.11373260738052	5.1
4	2.05686630369026	3.4000000000000004
5	1.4519056261343013	3.0
6	0.7864488808227466	1.95
7	1.02843315184513	2.9749999999999996
8	0.8469449485783425	2.8000000000000003
9	0.48396854204476714	1.7999999999999998
>10	2.782819116757411	19.825
>50	0.48396854204476714	14.05
>100	0.12099213551119178	5.7
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	110	2.75	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	80	2.0	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	69	1.725	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	65	1.625	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGC	62	1.55	RNA PCR Primer, Index 34 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	60	1.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	40	1.0	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	39	0.975	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	38	0.95	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	22	0.5499999999999999	RNA PCR Primer, Index 7 (100% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	18	0.44999999999999996	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	14	0.35000000000000003	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	12	0.3	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	12	0.3	No Hit
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	12	0.3	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	12	0.3	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	11	0.27499999999999997	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	11	0.27499999999999997	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	11	0.27499999999999997	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	11	0.27499999999999997	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	10	0.25	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	10	0.25	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	10	0.25	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	10	0.25	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC	9	0.22499999999999998	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTC	9	0.22499999999999998	RNA PCR Primer, Index 34 (100% over 44bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	8	0.2	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	8	0.2	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	8	0.2	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	8	0.2	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 32bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	7	0.17500000000000002	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	7	0.17500000000000002	No Hit
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TACCATGATGATAACATAGTTCAGCAGACTTAACTCTGATGAACAATCAT	5	0.125	No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGTGCC	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
ACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.7	0.0	0.0	0.0
2	0.0	1.7	0.0	0.0	0.0
3	0.0	1.825	0.0	0.0	0.0
4	0.0	1.825	0.0	0.0	0.0
5	0.0	1.825	0.0	0.0	0.0
6	0.0	1.9	0.0	0.0	0.0
7	0.0	2.225	0.0	0.0	0.0
8	0.0	2.35	0.0	0.0	0.0
9	0.0	2.475	0.0	0.0	0.0
10	0.0	2.75	0.0	0.0	0.0
11	0.0	2.85	0.0	0.0	0.0
12	0.0	2.95	0.0	0.0	0.0
13	0.0	3.175	0.0	0.0	0.0
14	0.0	3.4	0.0	0.0	0.0
15	0.0	3.725	0.0	0.0	0.0
16	0.0	4.7	0.0	0.0	0.0
17	0.0	5.275	0.0	0.0	0.0
18	0.0	6.175	0.0	0.0	0.0
19	0.0	6.8	0.0	0.0	0.0
20	0.0	7.5	0.0	0.0	0.0
21	0.0	11.95	0.0	0.0	0.0
22	0.0	22.4	0.0	0.0	0.0
23	0.0	46.3	0.0	0.0	0.0
24	0.0	57.45	0.0	0.0	0.0
25	0.0	62.925	0.0	0.0	0.0
26	0.0	64.425	0.0	0.0	0.0
27	0.0	65.4	0.0	0.0	0.0
28	0.0	65.975	0.0	0.0	0.0
29	0.0	66.55	0.0	0.0	0.0
30	0.0	66.825	0.0	0.0	0.0
31	0.0	67.375	0.0	0.0	0.0
32	0.0	67.875	0.0	0.0	0.0
33	0.0	68.7	0.0	0.0	0.0
34	0.0	71.475	0.0	0.0	0.0
35	0.0	72.5	0.0	0.0	0.0
36	0.0	73.175	0.0	0.0	0.0
37	0.0	73.9	0.0	0.0	0.0
38	0.0	74.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGG	20	6.952051E-4	44.000004	18
GTAGATT	20	6.952051E-4	44.000004	8
AACGCTG	40	5.315087E-9	44.000004	8
TTTTGGA	20	6.952051E-4	44.000004	19
GAGGTAG	40	5.315087E-9	44.000004	2
GGTGAGT	40	5.315087E-9	44.000004	17
ACGCTGT	40	5.315087E-9	44.000004	9
AATCCAG	20	6.952051E-4	44.000004	8
ATCCAGG	20	6.952051E-4	44.000004	9
AGTAGAT	20	6.952051E-4	44.000004	7
TTCAACG	40	5.315087E-9	44.000004	5
AGGATAG	20	6.952051E-4	44.000004	13
TCGGTGA	40	5.315087E-9	44.000004	15
ATAGGCT	20	6.952051E-4	44.000004	16
CATTCAA	40	5.315087E-9	44.000004	3
TCAACGC	40	5.315087E-9	44.000004	6
GATTGTA	20	6.952051E-4	44.000004	11
ACATTCA	40	5.315087E-9	44.000004	2
GGATAGG	20	6.952051E-4	44.000004	14
CGTGGAA	20	6.952051E-4	44.000004	21
>>END_MODULE
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
Read 186589 spots for SRR5030854.sra
Written 186589 spots for SRR5030854.sra
SRR ids: ['SRR5030854.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_kf4ec3e9
SRR5030854.sra spots: 2239068
blocks: [[1, 186589], [186590, 373178], [373179, 559767], [559768, 746356], [746357, 932945], [932946, 1119534], [1119535, 1306123], [1306124, 1492712], [1492713, 1679301], [1679302, 1865890], [1865891, 2052479], [2052480, 2239068]]
SRR5030854 file size 387001
SRR5030854 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030854 SRR5030854_1.fastq
Input file:	SRR5030854_1.fastq
trimmed:	SRR5030854-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:29:07 2020 >> started

Wed Mar  4 06:29:10 2020 >> done (2.837s)
2239068 reads processed; of these:
    105 ( 0.00%) short reads filtered out after trimming by size control
      2 ( 0.00%) empty reads filtered out after trimming by size control
2238961 (100.00%) reads available; of these:
 136080 ( 6.08%) trimmed reads available after processing
2102881 (93.92%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     47	  0.00%
 19	     44	  0.00%
 20	    101	  0.00%
 21	    125	  0.01%
 22	    207	  0.01%
 23	    227	  0.01%
 24	    261	  0.01%
 25	    371	  0.02%
 26	    346	  0.02%
 27	    451	  0.02%
 28	    554	  0.02%
 29	    859	  0.04%
 30	    637	  0.03%
 31	    711	  0.03%
 32	    807	  0.04%
 33	   1025	  0.05%
 34	   1690	  0.08%
 35	   1811	  0.08%
 36	   1491	  0.07%
 37	   1602	  0.07%
 38	   1852	  0.08%
 39	   2316	  0.10%
 40	   2594	  0.12%
 41	   2686	  0.12%
 42	   3434	  0.15%
 43	   4548	  0.20%
 44	   7237	  0.32%
 45	   9781	  0.44%
 46	   9397	  0.42%
 47	  13477	  0.60%
 48	  25830	  1.15%
 49	  39561	  1.77%
 50	2102881	 93.92%
2238961 reads passed initial QC


criterion=sequence-density
sequence-density=75.05
sequence-density-rank=1
fanout-score=21.30
fanout-score-rank=1
prefix-density=76.14
prefix-fanout=21.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC


criterion=fanout-score
sequence-density=75.05
sequence-density-rank=1
fanout-score=21.30
fanout-score-rank=1
prefix-density=76.14
prefix-fanout=21.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC -o SRR5030854 -
Input file:	STDIN
trimmed:	SRR5030854-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:29:30 2020 >> started

Wed Mar  4 06:29:36 2020 >> done (5.788s)
2180041 reads processed; of these:
 106024 ( 4.86%) short reads filtered out after trimming by size control
  32350 ( 1.48%) empty reads filtered out after trimming by size control
2041667 (93.65%) reads available; of these:
1718355 (84.16%) trimmed reads available after processing
 323312 (15.84%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  11829	  0.58%
 19	  22618	  1.11%
 20	 104708	  5.13%
 21	 222800	 10.91%
 22	 539923	 26.45%
 23	 255662	 12.52%
 24	 119484	  5.85%
 25	  31435	  1.54%
 26	  16964	  0.83%
 27	  12386	  0.61%
 28	  13950	  0.68%
 29	  11272	  0.55%
 30	  15478	  0.76%
 31	  19480	  0.95%
 32	  20491	  1.00%
 33	  54328	  2.66%
 34	  21521	  1.05%
 35	  17264	  0.85%
 36	  20776	  1.02%
 37	  10673	  0.52%
 38	  13215	  0.65%
 39	  12425	  0.61%
 40	  17801	  0.87%
 41	  23955	  1.17%
 42	  28687	  1.41%
 43	  21430	  1.05%
 44	  15600	  0.76%
 45	  26137	  1.28%
 46	  20080	  0.98%
 47	  15789	  0.77%
 48	   3106	  0.15%
 49	   8387	  0.41%
 50	 292013	 14.30%


criterion=sequence-density
sequence-density=6.63
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=36
fanout-score=73.12
fanout-score-rank=1
prefix-density=1.59
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:30:06
                             Started mapping on |	Mar 04 06:30:07
                                    Finished on |	Mar 04 06:30:31
       Mapping speed, Million of reads per hour |	315.09

                          Number of input reads |	2100587
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1091154
                        Uniquely mapped reads % |	51.95%
                          Average mapped length |	27.72
                       Number of splices: Total |	1175
            Number of splices: Annotated (sjdb) |	141
                       Number of splices: GT/AG |	1089
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	70
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.74
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.11
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	648693
             % of reads mapped to multiple loci |	30.88%
        Number of reads mapped to too many loci |	46502
             % of reads mapped to too many loci |	2.21%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.38%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	360740	360740	360740
N_multimapping	648693	648693	648693
N_noFeature	54103	67979	795294
N_ambiguous	586055	334100	14325
UnstrandedReadsAssigned:450996 PositiveStrandReadsAssigned:689075 NegativeStrandReadsAssigned:281535
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030854 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030854-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,100,587 reads, 477,510 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,106 rounds

 109283 SRR5030854.ke.tsv
  52636 SRR5030854.se.tsv
 161919 total
==> SRR5030854.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030854.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030854 completed mapping pipeline successfully
