Starting /dee2/code/volunteer_pipeline.sh SRR5030960
    current disk space = 160579108864
    free memory = 56712744 
SRR5030960 SRAfilesize
9472c7a748969d134102ed183d6b7b37  SRR5030960.sra
SRR5030960.sra file validated
SRR5030960 is single end
SRR5030960 is conventional basespace
SRR5030960 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3595	33.0	31.0	34.0	31.0	34.0
2	32.66025	34.0	31.0	34.0	31.0	34.0
3	32.73025	34.0	31.0	34.0	31.0	34.0
4	36.2185	37.0	37.0	37.0	35.0	37.0
5	36.28625	37.0	37.0	37.0	35.0	37.0
6	36.1165	37.0	36.0	37.0	35.0	37.0
7	36.122	37.0	35.0	37.0	35.0	37.0
8	36.136	37.0	36.0	37.0	35.0	37.0
9	37.9125	39.0	38.0	39.0	35.0	39.0
10	37.84725	39.0	38.0	39.0	35.0	39.0
11	37.743	39.0	38.0	39.0	35.0	39.0
12	37.6875	39.0	38.0	39.0	35.0	39.0
13	37.6475	39.0	38.0	39.0	35.0	39.0
14	39.1275	40.0	39.0	41.0	36.0	41.0
15	39.20575	41.0	39.0	41.0	36.0	41.0
16	39.098	40.0	39.0	41.0	36.0	41.0
17	39.15525	40.0	39.0	41.0	36.0	41.0
18	39.2245	41.0	39.0	41.0	36.0	41.0
19	39.27	41.0	39.0	41.0	36.0	41.0
20	39.06175	40.0	39.0	41.0	36.0	41.0
21	39.07175	40.0	39.0	41.0	36.0	41.0
22	39.25375	41.0	39.0	41.0	36.0	41.0
23	39.16325	41.0	39.0	41.0	36.0	41.0
24	38.3835	40.0	38.0	41.0	34.0	41.0
25	38.49325	40.0	38.0	41.0	34.0	41.0
26	38.6855	40.0	38.0	41.0	34.0	41.0
27	38.73675	40.0	38.0	41.0	35.0	41.0
28	38.41625	40.0	38.0	41.0	34.0	41.0
29	37.816	40.0	38.0	41.0	32.0	41.0
30	37.92875	40.0	38.0	41.0	32.0	41.0
31	38.413	40.0	38.0	41.0	33.0	41.0
32	38.4675	40.0	38.0	41.0	34.0	41.0
33	37.20025	40.0	37.0	41.0	31.0	41.0
34	37.273	39.0	37.0	41.0	30.0	41.0
35	37.11775	39.0	37.0	40.0	31.0	41.0
36	33.675	38.0	30.0	40.0	18.0	41.0
37	35.684	38.0	34.0	40.0	30.0	41.0
38	36.94075	39.0	36.0	40.0	32.0	41.0
39	36.93475	39.0	36.0	40.0	31.0	41.0
40	36.899	39.0	36.0	40.0	31.0	41.0
41	36.5555	39.0	36.0	40.0	30.0	41.0
42	36.48675	39.0	36.0	40.0	30.0	41.0
43	36.51475	39.0	35.0	40.0	30.0	41.0
44	36.65975	39.0	36.0	41.0	31.0	41.0
45	36.14825	39.0	35.0	40.0	30.0	41.0
46	33.81325	37.0	33.0	40.0	23.0	41.0
47	34.7745	38.0	34.0	40.0	26.0	41.0
48	34.91425	38.0	34.0	40.0	28.0	41.0
49	34.13325	38.0	34.0	40.0	24.0	41.0
50	30.6815	34.0	29.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	1.0
14	3.0
15	1.0
16	1.0
17	3.0
18	1.0
19	1.0
20	7.0
21	4.0
22	4.0
23	13.0
24	17.0
25	34.0
26	31.0
27	25.0
28	30.0
29	48.0
30	46.0
31	74.0
32	94.0
33	104.0
34	158.0
35	218.0
36	334.0
37	592.0
38	1031.0
39	1123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.927855711422845	24.9248496993988	41.78356713426854	19.36372745490982
2	23.25	26.724999999999998	21.925	28.1
3	24.681170292573142	18.079519879969993	23.93098274568642	33.30832708177044
4	32.65	27.6	16.05	23.7
5	21.0	37.0	24.675	17.325
6	27.800000000000004	17.724999999999998	39.025	15.45
7	23.875	18.775	22.6	34.75
8	23.325000000000003	30.325000000000003	16.625	29.725
9	24.25	30.0	27.875	17.875
10	26.650000000000002	16.925	30.125	26.3
11	31.874999999999996	18.775	14.899999999999999	34.449999999999996
12	18.35	22.3	29.775000000000002	29.575000000000003
13	28.849999999999998	18.9	29.65	22.6
14	26.450000000000003	30.15	22.650000000000002	20.75
15	34.075	22.8	26.05	17.075000000000003
16	17.95	21.2	26.224999999999998	34.625
17	24.775	22.525000000000002	34.5	18.2
18	23.825	17.875	33.85	24.45
19	33.225	16.8	31.15	18.825
20	39.574999999999996	17.575	25.275	17.575
21	24.275	22.025	21.925	31.775
22	29.475	14.35	40.075	16.1
23	27.975	14.124999999999998	36.449999999999996	21.45
24	44.675	17.65	24.525	13.15
25	43.8	22.15	16.75	17.299999999999997
26	29.75	37.3	17.325	15.625
27	21.55	38.95	21.525	17.974999999999998
28	17.625	25.15	39.925	17.299999999999997
29	13.600000000000001	18.224999999999998	48.475	19.7
30	13.350000000000001	12.025	31.574999999999996	43.05
31	18.275	9.475	42.275	29.975
32	28.125	10.775	21.3	39.800000000000004
33	43.6	13.100000000000001	17.299999999999997	26.0
34	48.775	12.525	19.975	18.725
35	54.6	10.75	19.575	15.075
36	41.4	11.5	30.099999999999998	17.0
37	39.925	14.124999999999998	21.05	24.9
38	24.45	18.05	16.6	40.9
39	21.175	22.875	14.05	41.9
40	20.599999999999998	39.525	16.825000000000003	23.05
41	28.1	39.125	13.925	18.85
42	43.175000000000004	27.250000000000004	16.125	13.450000000000001
43	44.125	28.525	13.275	14.075
44	31.7	41.099999999999994	11.799999999999999	15.4
45	21.675	44.975	15.85	17.5
46	17.299999999999997	28.7	19.6	34.4
47	13.4	19.275000000000002	36.35	30.975
48	16.0	15.275	22.225	46.5
49	17.65	20.625	19.025	42.699999999999996
50	21.275	31.474999999999998	17.424999999999997	29.825000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	2.0
15	1.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	1.0
22	2.0
23	1.5
24	1.0
25	1.0
26	1.0
27	1.0
28	1.0
29	0.5
30	0.0
31	16.0
32	32.0
33	37.0
34	42.0
35	50.5
36	59.0
37	71.0
38	83.0
39	88.0
40	93.0
41	92.5
42	92.0
43	122.0
44	152.0
45	183.0
46	214.0
47	280.5
48	347.0
49	418.0
50	489.0
51	614.0
52	739.0
53	561.0
54	383.0
55	418.5
56	454.0
57	359.5
58	265.0
59	183.0
60	101.0
61	76.0
62	51.0
63	76.0
64	101.0
65	70.0
66	39.0
67	29.0
68	19.0
69	38.0
70	57.0
71	102.0
72	147.0
73	82.0
74	17.0
75	9.5
76	2.0
77	2.5
78	3.0
79	5.5
80	8.0
81	5.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.074999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20465890183029	34.8
2	9.539656128674432	8.6
3	3.6605657237936775	4.95
4	2.0521353300055463	3.6999999999999997
5	1.1092623405435387	2.5
6	0.9983361064891847	2.7
7	0.8319467554076538	2.625
8	0.9428729894620078	3.4000000000000004
9	0.33277870216306155	1.35
>10	2.8840820854132	21.875
>50	0.3882418191902385	10.975
>100	0.055463117027176934	2.5250000000000004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	80	2.0	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	61	1.525	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	60	1.5	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	57	1.425	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	39	0.975	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	36	0.8999999999999999	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	34	0.8500000000000001	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG	32	0.8	RNA PCR Primer, Index 2 (97% over 35bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 33bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	25	0.625	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	23	0.575	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC	22	0.5499999999999999	RNA PCR Primer, Index 13 (100% over 44bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	20	0.5	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (96% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	17	0.42500000000000004	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	16	0.4	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	16	0.4	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	16	0.4	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	14	0.35000000000000003	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT	11	0.27499999999999997	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	11	0.27499999999999997	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	11	0.27499999999999997	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	10	0.25	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	10	0.25	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	10	0.25	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	10	0.25	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
GTCTGATGATGAGCCCATTGCCCTGAGCTCTTGTGATGCTGGCTTCAAAG	9	0.22499999999999998	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC	9	0.22499999999999998	RNA PCR Primer, Index 13 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	9	0.22499999999999998	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	8	0.2	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	8	0.2	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	8	0.2	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	8	0.2	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	7	0.17500000000000002	No Hit
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	7	0.17500000000000002	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	7	0.17500000000000002	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCA	7	0.17500000000000002	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 24bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	7	0.17500000000000002	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTAT	6	0.15	RNA PCR Primer, Index 13 (100% over 48bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (96% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TGCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTG	5	0.125	No Hit
TGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGAC	5	0.125	No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGTGGAATTCTCG	5	0.125	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	5	0.125	No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGCCAATCTAGCCAT	5	0.125	No Hit
CTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTC	5	0.125	No Hit
GGCCAATGATGAGGAGCATGGGGTTTCTGACCGAGGACAGTGATTAAACC	5	0.125	No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.25	0.0	0.0	0.0
3	0.0	0.45	0.0	0.0	0.0
4	0.0	0.45	0.0	0.0	0.0
5	0.0	0.5	0.0	0.0	0.0
6	0.0	0.5	0.0	0.0	0.0
7	0.0	1.35	0.0	0.0	0.0
8	0.0	1.575	0.0	0.0	0.0
9	0.0	1.675	0.0	0.0	0.0
10	0.0	1.825	0.0	0.0	0.0
11	0.0	2.075	0.0	0.0	0.0
12	0.0	2.275	0.0	0.0	0.0
13	0.0	2.475	0.0	0.0	0.0
14	0.0	2.775	0.0	0.0	0.0
15	0.0	3.225	0.0	0.0	0.0
16	0.0	4.35	0.0	0.0	0.0
17	0.0	4.925	0.0	0.0	0.0
18	0.0	6.2	0.0	0.0	0.0
19	0.0	6.45	0.0	0.0	0.0
20	0.0	7.225	0.0	0.0	0.0
21	0.0	11.525	0.0	0.0	0.0
22	0.0	19.225	0.0	0.0	0.0
23	0.0	40.125	0.0	0.0	0.0
24	0.0	49.375	0.0	0.0	0.0
25	0.0	54.675	0.0	0.0	0.0
26	0.0	56.425	0.0	0.0	0.0
27	0.0	57.275	0.0	0.0	0.0
28	0.0	57.925	0.0	0.0	0.0
29	0.0	58.75	0.0	0.0	0.0
30	0.0	59.175	0.0	0.0	0.0
31	0.0	59.925	0.0	0.0	0.0
32	0.0	60.75	0.0	0.0	0.0
33	0.0	61.675	0.0	0.0	0.0
34	0.0	64.3	0.0	0.0	0.0
35	0.0	65.35	0.0	0.0	0.0
36	0.0	66.1	0.0	0.0	0.0
37	0.0	66.9	0.0	0.0	0.0
38	0.0	67.575	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
ACTGTTG	20	6.952051E-4	44.000004	18
GGTGAGT	20	6.952051E-4	44.000004	17
CTGAGAC	20	6.952051E-4	44.000004	4
CTTTAAC	20	6.952051E-4	44.000004	12
AACCTGT	20	6.952051E-4	44.000004	16
TAACCTG	20	6.952051E-4	44.000004	15
ACCCTTT	20	6.952051E-4	44.000004	9
GGCTTGG	20	6.952051E-4	44.000004	19
CGGTGAG	20	6.952051E-4	44.000004	16
CCTTTAA	20	6.952051E-4	44.000004	11
GACCCTT	20	6.952051E-4	44.000004	8
AGACCCT	20	6.952051E-4	44.000004	7
CCCTTTA	20	6.952051E-4	44.000004	10
TTAACCT	20	6.952051E-4	44.000004	14
TTTAACC	20	6.952051E-4	44.000004	13
GAGACCC	20	6.952051E-4	44.000004	6
TCCCTGA	20	6.952051E-4	44.000004	1
AACGCTG	25	3.6958358E-5	44.0	8
GTTGAAG	25	3.6958358E-5	44.0	10
>>END_MODULE
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228460 spots for SRR5030960.sra
Written 228460 spots for SRR5030960.sra
Read 228465 spots for SRR5030960.sra
Written 228465 spots for SRR5030960.sra
SRR ids: ['SRR5030960.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_poqa1u35
SRR5030960.sra spots: 2741525
blocks: [[1, 228460], [228461, 456920], [456921, 685380], [685381, 913840], [913841, 1142300], [1142301, 1370760], [1370761, 1599220], [1599221, 1827680], [1827681, 2056140], [2056141, 2284600], [2284601, 2513060], [2513061, 2741525]]
SRR5030960 file size 474089
SRR5030960 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030960 SRR5030960_1.fastq
Input file:	SRR5030960_1.fastq
trimmed:	SRR5030960-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:54:25 2020 >> started

Wed Mar  4 06:54:27 2020 >> done (1.886s)
2741525 reads processed; of these:
    131 ( 0.00%) short reads filtered out after trimming by size control
     11 ( 0.00%) empty reads filtered out after trimming by size control
2741383 (99.99%) reads available; of these:
 206134 ( 7.52%) trimmed reads available after processing
2535249 (92.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     47	  0.00%
 19	     65	  0.00%
 20	    147	  0.01%
 21	    171	  0.01%
 22	    257	  0.01%
 23	    342	  0.01%
 24	    397	  0.01%
 25	    516	  0.02%
 26	    431	  0.02%
 27	    583	  0.02%
 28	    712	  0.03%
 29	    939	  0.03%
 30	    801	  0.03%
 31	   1041	  0.04%
 32	   1490	  0.05%
 33	   2348	  0.09%
 34	   6066	  0.22%
 35	   3032	  0.11%
 36	   2192	  0.08%
 37	   2778	  0.10%
 38	   3644	  0.13%
 39	   3905	  0.14%
 40	   5463	  0.20%
 41	   4338	  0.16%
 42	   7006	  0.26%
 43	   5950	  0.22%
 44	  13452	  0.49%
 45	  14732	  0.54%
 46	  14688	  0.54%
 47	  17532	  0.64%
 48	  36806	  1.34%
 49	  54263	  1.98%
 50	2535249	 92.48%
2741383 reads passed initial QC


criterion=sequence-density
sequence-density=66.29
sequence-density-rank=1
fanout-score=21.24
fanout-score-rank=2
prefix-density=69.48
prefix-fanout=20.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC


criterion=fanout-score
sequence-density=1.82
sequence-density-rank=5
fanout-score=120.69
fanout-score-rank=1
prefix-density=68.25
prefix-fanout=3.2
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC -o SRR5030960 -
Input file:	STDIN
trimmed:	SRR5030960-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:54:41 2020 >> started

Wed Mar  4 06:54:45 2020 >> done (4.243s)
2659551 reads processed; of these:
 162625 ( 6.11%) short reads filtered out after trimming by size control
   9917 ( 0.37%) empty reads filtered out after trimming by size control
2487009 (93.51%) reads available; of these:
1975700 (79.44%) trimmed reads available after processing
 511309 (20.56%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12425	  0.50%
 19	  21016	  0.85%
 20	 117497	  4.72%
 21	 202811	  8.15%
 22	 550951	 22.15%
 23	 246802	  9.92%
 24	 130275	  5.24%
 25	  42167	  1.70%
 26	  24718	  0.99%
 27	  18212	  0.73%
 28	  19228	  0.77%
 29	  15928	  0.64%
 30	  20772	  0.84%
 31	  28526	  1.15%
 32	  30008	  1.21%
 33	  68020	  2.74%
 34	  29178	  1.17%
 35	  24016	  0.97%
 36	  24732	  0.99%
 37	  19899	  0.80%
 38	  21253	  0.85%
 39	  22630	  0.91%
 40	  36743	  1.48%
 41	  43344	  1.74%
 42	  52369	  2.11%
 43	  38261	  1.54%
 44	  29717	  1.19%
 45	  48546	  1.95%
 46	  42654	  1.72%
 47	  30846	  1.24%
 48	   5718	  0.23%
 49	  16023	  0.64%
 50	 451724	 18.16%


criterion=sequence-density
sequence-density=5.14
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.66
sequence-density-rank=16
fanout-score=10.97
fanout-score-rank=1
prefix-density=7.25
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:55:08
                             Started mapping on |	Mar 04 06:55:08
                                    Finished on |	Mar 04 06:55:31
       Mapping speed, Million of reads per hour |	402.08

                          Number of input reads |	2568841
                      Average input read length |	32
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1446962
                        Uniquely mapped reads % |	56.33%
                          Average mapped length |	30.43
                       Number of splices: Total |	1654
            Number of splices: Annotated (sjdb) |	235
                       Number of splices: GT/AG |	1501
                       Number of splices: GC/AG |	34
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	118
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.55
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	619572
             % of reads mapped to multiple loci |	24.12%
        Number of reads mapped to too many loci |	78162
             % of reads mapped to too many loci |	3.04%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.54%
                     % of reads unmapped: other |	1.97%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	502307	502307	502307
N_multimapping	619572	619572	619572
N_noFeature	77524	104512	1037906
N_ambiguous	759617	409454	22457
UnstrandedReadsAssigned:609821 PositiveStrandReadsAssigned:932996 NegativeStrandReadsAssigned:386599
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=22 echo kmer=19
SRR5030960 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030960-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,568,841 reads, 624,909 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,096 rounds

 109283 SRR5030960.ke.tsv
  52636 SRR5030960.se.tsv
 161919 total
==> SRR5030960.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030960.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030960 completed mapping pipeline successfully
