Starting /dee2/code/volunteer_pipeline.sh SRR5030863
    current disk space = 160679378944
    free memory = 73968208 
SRR5030863 SRAfilesize
bfbc3f58841676e8039f692bb5d655cc  SRR5030863.sra
SRR5030863.sra file validated
SRR5030863 is single end
SRR5030863 is conventional basespace
SRR5030863 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.376	33.0	31.0	34.0	31.0	34.0
2	32.71475	34.0	31.0	34.0	31.0	34.0
3	32.7075	34.0	31.0	34.0	31.0	34.0
4	36.2605	37.0	37.0	37.0	35.0	37.0
5	36.35575	37.0	37.0	37.0	35.0	37.0
6	36.18725	37.0	36.0	37.0	35.0	37.0
7	36.1245	37.0	35.0	37.0	35.0	37.0
8	36.2065	37.0	36.0	37.0	35.0	37.0
9	37.95425	39.0	38.0	39.0	35.0	39.0
10	37.9505	39.0	38.0	39.0	35.0	39.0
11	37.85275	39.0	38.0	39.0	35.0	39.0
12	37.72625	39.0	38.0	39.0	35.0	39.0
13	37.8205	39.0	38.0	39.0	35.0	39.0
14	39.17075	40.0	39.0	41.0	36.0	41.0
15	39.21	40.0	39.0	41.0	36.0	41.0
16	39.202	40.0	39.0	41.0	36.0	41.0
17	39.2035	40.0	39.0	41.0	36.0	41.0
18	39.30175	41.0	39.0	41.0	36.0	41.0
19	39.21925	41.0	39.0	41.0	36.0	41.0
20	39.1505	40.0	39.0	41.0	36.0	41.0
21	39.15175	40.0	39.0	41.0	36.0	41.0
22	39.2585	41.0	39.0	41.0	36.0	41.0
23	39.2135	41.0	39.0	41.0	36.0	41.0
24	38.43925	40.0	38.0	41.0	34.0	41.0
25	38.53025	40.0	38.0	41.0	34.0	41.0
26	38.72925	40.0	38.0	41.0	34.0	41.0
27	38.88875	40.0	39.0	41.0	35.0	41.0
28	38.59075	40.0	38.0	41.0	34.0	41.0
29	38.14475	40.0	38.0	41.0	33.0	41.0
30	38.34275	40.0	38.0	41.0	33.0	41.0
31	38.7835	40.0	38.0	41.0	35.0	41.0
32	38.79925	40.0	38.0	41.0	35.0	41.0
33	37.66425	40.0	38.0	41.0	32.0	41.0
34	37.495	40.0	37.0	41.0	31.0	41.0
35	37.47625	39.0	37.0	41.0	32.0	41.0
36	32.912	38.0	30.0	40.0	16.0	41.0
37	35.44375	38.0	34.0	40.0	29.0	41.0
38	36.891	39.0	36.0	40.0	31.0	41.0
39	37.00775	39.0	37.0	40.0	31.0	41.0
40	37.123	39.0	37.0	40.0	32.0	41.0
41	36.657	39.0	36.0	40.0	30.0	41.0
42	36.7605	39.0	36.0	40.0	31.0	41.0
43	36.764	39.0	36.0	40.0	31.0	41.0
44	37.0125	39.0	36.0	41.0	32.0	41.0
45	36.48275	39.0	36.0	40.0	31.0	41.0
46	34.0815	38.0	33.0	40.0	23.0	41.0
47	35.24475	38.0	34.0	40.0	28.0	41.0
48	35.34275	38.0	35.0	40.0	28.0	41.0
49	34.342	38.0	34.0	40.0	26.0	41.0
50	30.33775	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	3.0
15	1.0
16	1.0
17	0.0
18	1.0
19	1.0
20	4.0
21	10.0
22	6.0
23	13.0
24	9.0
25	20.0
26	20.0
27	24.0
28	26.0
29	31.0
30	54.0
31	76.0
32	85.0
33	129.0
34	159.0
35	223.0
36	365.0
37	564.0
38	1043.0
39	1130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.882647943831495	24.799398194583752	48.771313941825476	15.546639919759278
2	22.675	26.224999999999998	18.925	32.175
3	25.025	21.375	23.275000000000002	30.325000000000003
4	29.525000000000002	29.5	14.000000000000002	26.974999999999998
5	19.400000000000002	43.45	25.25	11.899999999999999
6	33.25	18.3	35.375	13.075000000000001
7	18.125	19.375	25.324999999999996	37.175000000000004
8	25.900000000000002	30.85	14.35	28.9
9	21.65	33.875	30.175	14.299999999999999
10	22.8	20.875	30.65	25.674999999999997
11	28.825	14.000000000000002	16.400000000000002	40.775
12	15.45	19.075	31.4	34.075
13	31.4	20.75	28.449999999999996	19.400000000000002
14	29.549999999999997	32.95	20.075000000000003	17.424999999999997
15	32.375	25.674999999999997	26.825	15.125
16	17.45	22.475	21.575	38.5
17	23.825	21.55	40.5	14.124999999999998
18	23.849999999999998	20.325	30.525000000000002	25.3
19	36.275	15.325	30.175	18.224999999999998
20	40.825	17.925	26.85	14.399999999999999
21	23.225	20.275000000000002	27.950000000000003	28.549999999999997
22	30.375000000000004	13.375	44.4	11.85
23	27.675	14.174999999999999	40.725	17.424999999999997
24	49.65	16.375	25.3	8.674999999999999
25	47.325	24.375	15.25	13.05
26	27.800000000000004	44.1	16.275000000000002	11.825
27	17.424999999999997	44.425	24.975	13.175
28	12.925	24.65	46.400000000000006	16.025
29	10.25	15.675	54.85	19.225
30	11.5	9.1	34.575	44.824999999999996
31	16.650000000000002	7.5	44.45	31.4
32	28.575	9.0	20.3	42.125
33	51.800000000000004	10.825	15.65	21.725
34	56.325	9.825000000000001	17.95	15.9
35	60.224999999999994	7.3999999999999995	19.25	13.125
36	44.275	8.35	31.624999999999996	15.75
37	43.6	10.725	19.35	26.325
38	23.125	16.6	14.149999999999999	46.125
39	19.25	24.275	10.05	46.425
40	18.099999999999998	44.55	13.125	24.224999999999998
41	27.025	45.25	11.125	16.6
42	48.225	29.925	11.575000000000001	10.274999999999999
43	46.925	32.25	10.0	10.825
44	30.725	47.125	8.375	13.775
45	18.4	49.725	13.55	18.325
46	12.174999999999999	28.849999999999998	19.05	39.925
47	9.5	18.7	37.25	34.55
48	11.4	14.649999999999999	20.849999999999998	53.1
49	14.85	20.474999999999998	16.650000000000002	48.025
50	19.2	34.25	15.35	31.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	1.0
22	1.0
23	1.0
24	1.0
25	2.0
26	3.0
27	4.0
28	5.0
29	5.5
30	6.0
31	15.5
32	25.0
33	27.5
34	30.0
35	37.5
36	45.0
37	64.0
38	83.0
39	75.0
40	67.0
41	62.5
42	58.0
43	90.0
44	122.0
45	172.0
46	222.0
47	324.5
48	427.0
49	516.5
50	606.0
51	703.5
52	801.0
53	612.0
54	423.0
55	454.5
56	486.0
57	355.5
58	225.0
59	149.5
60	74.0
61	60.0
62	46.0
63	65.0
64	84.0
65	60.5
66	37.0
67	28.5
68	20.0
69	22.5
70	25.0
71	37.5
72	50.0
73	28.5
74	7.0
75	5.5
76	4.0
77	3.0
78	2.0
79	3.5
80	5.0
81	4.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1548946716233	30.325000000000003
2	10.223048327137546	8.25
3	3.6555142503097895	4.425
4	2.41635687732342	3.9
5	1.3630731102850062	2.75
6	1.486988847583643	3.5999999999999996
7	0.6815365551425031	1.925
8	0.8054522924411399	2.6
9	0.6195786864931847	2.25
>10	3.0978934324659235	24.075
>50	0.43370508054522927	12.925
>100	0.061957868649318466	2.9749999999999996
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	98	2.45	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	92	2.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	43	1.075	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	42	1.05	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	41	1.0250000000000001	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	27	0.675	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	25	0.625	RNA PCR Primer, Index 5 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	20	0.5	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	19	0.475	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTC	19	0.475	RNA PCR Primer, Index 46 (100% over 44bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	18	0.44999999999999996	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	18	0.44999999999999996	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	17	0.42500000000000004	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	14	0.35000000000000003	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	14	0.35000000000000003	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	12	0.3	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	12	0.3	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	11	0.27499999999999997	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	11	0.27499999999999997	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	10	0.25	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	9	0.22499999999999998	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	9	0.22499999999999998	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	9	0.22499999999999998	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTCGTATGC	8	0.2	RNA PCR Primer, Index 46 (100% over 50bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	8	0.2	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	8	0.2	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	7	0.17500000000000002	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	6	0.15	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG	6	0.15	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT	6	0.15	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	6	0.15	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAAT	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
CTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAG	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	5	0.125	RNA PCR Primer, Index 5 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	5	0.125	RNA PCR Primer, Index 1 (96% over 29bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	5	0.125	No Hit
TTACTTGATGATAGTAAAAGATCTGATGTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
GCTCGACTCATGGTTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	5	0.125	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTGTCTCGCTGACGCCATCACCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.3	0.0	0.0	0.0
3	0.0	0.4	0.0	0.0	0.0
4	0.0	0.425	0.0	0.0	0.0
5	0.0	0.475	0.0	0.0	0.0
6	0.0	0.525	0.0	0.0	0.0
7	0.0	1.025	0.0	0.0	0.0
8	0.0	1.15	0.0	0.0	0.0
9	0.0	1.2	0.0	0.0	0.0
10	0.0	1.25	0.0	0.0	0.0
11	0.0	1.475	0.0	0.0	0.0
12	0.0	1.625	0.0	0.0	0.0
13	0.0	1.775	0.0	0.0	0.0
14	0.0	2.0	0.0	0.0	0.0
15	0.0	2.3	0.0	0.0	0.0
16	0.0	3.35	0.0	0.0	0.0
17	0.0	3.75	0.0	0.0	0.0
18	0.0	4.7	0.0	0.0	0.0
19	0.0	5.075	0.0	0.0	0.0
20	0.0	6.2	0.0	0.0	0.0
21	0.0	12.2	0.0	0.0	0.0
22	0.0	22.85	0.0	0.0	0.0
23	0.0	49.65	0.0	0.0	0.0
24	0.0	60.525	0.0	0.0	0.0
25	0.0	66.825	0.0	0.0	0.0
26	0.0	68.25	0.0	0.0	0.0
27	0.0	68.925	0.0	0.0	0.0
28	0.0	69.75	0.0	0.0	0.0
29	0.0	70.375	0.0	0.0	0.0
30	0.0	70.8	0.0	0.0	0.0
31	0.0	71.55	0.0	0.0	0.0
32	0.0	71.9	0.0	0.0	0.0
33	0.0	72.6	0.0	0.0	0.0
34	0.0	74.475	0.0	0.0	0.0
35	0.0	75.2	0.0	0.0	0.0
36	0.0	75.725	0.0	0.0	0.0
37	0.0	76.225	0.0	0.0	0.0
38	0.0	76.45	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATGAT	20	6.952051E-4	44.000004	6
CTGAGCT	45	2.728484E-10	44.000004	11
TTGTATA	20	6.952051E-4	44.000004	13
ATGATGA	20	6.952051E-4	44.000004	5
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
TCGGATC	45	2.728484E-10	44.000004	1
TAGTTTG	20	6.952051E-4	44.000004	18
CGGATCC	45	2.728484E-10	44.000004	2
ACATTCA	40	5.315087E-9	44.000004	2
GTATAGT	20	6.952051E-4	44.000004	15
ATAGTTT	20	6.952051E-4	44.000004	17
GATCCGT	45	2.728484E-10	44.000004	4
GGCTTGG	20	6.952051E-4	44.000004	19
TTGGCTT	20	6.952051E-4	44.000004	17
TCCGTCT	45	2.728484E-10	44.000004	6
ATCCGTC	45	2.728484E-10	44.000004	5
TGTATAG	20	6.952051E-4	44.000004	14
TGAGCTT	45	2.728484E-10	44.000004	12
>>END_MODULE
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268361 spots for SRR5030863.sra
Written 268361 spots for SRR5030863.sra
Read 268368 spots for SRR5030863.sra
Written 268368 spots for SRR5030863.sra
SRR ids: ['SRR5030863.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_99v7akf2
SRR5030863.sra spots: 3220339
blocks: [[1, 268361], [268362, 536722], [536723, 805083], [805084, 1073444], [1073445, 1341805], [1341806, 1610166], [1610167, 1878527], [1878528, 2146888], [2146889, 2415249], [2415250, 2683610], [2683611, 2951971], [2951972, 3220339]]
SRR5030863 file size 557076
SRR5030863 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030863 SRR5030863_1.fastq
Input file:	SRR5030863_1.fastq
trimmed:	SRR5030863-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:31:33 2020 >> started

Wed Mar  4 06:31:35 2020 >> done (2.013s)
3220339 reads processed; of these:
    186 ( 0.01%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
3220145 (99.99%) reads available; of these:
 250013 ( 7.76%) trimmed reads available after processing
2970132 (92.24%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     61	  0.00%
 19	     64	  0.00%
 20	    193	  0.01%
 21	    241	  0.01%
 22	    359	  0.01%
 23	    422	  0.01%
 24	    470	  0.01%
 25	    593	  0.02%
 26	    550	  0.02%
 27	    685	  0.02%
 28	    770	  0.02%
 29	   1135	  0.04%
 30	    906	  0.03%
 31	   1123	  0.03%
 32	   1578	  0.05%
 33	   2665	  0.08%
 34	   8434	  0.26%
 35	   4089	  0.13%
 36	   2997	  0.09%
 37	   3162	  0.10%
 38	   4860	  0.15%
 39	   4328	  0.13%
 40	   7129	  0.22%
 41	   5349	  0.17%
 42	   8704	  0.27%
 43	   7033	  0.22%
 44	  19160	  0.60%
 45	  17034	  0.53%
 46	  16336	  0.51%
 47	  19513	  0.61%
 48	  47543	  1.48%
 49	  62527	  1.94%
 50	2970132	 92.24%
3220145 reads passed initial QC


criterion=sequence-density
sequence-density=75.97
sequence-density-rank=1
fanout-score=20.22
fanout-score-rank=2
prefix-density=78.07
prefix-fanout=19.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTCGTATGCC


criterion=fanout-score
sequence-density=1.69
sequence-density-rank=7
fanout-score=131.76
fanout-score-rank=1
prefix-density=77.17
prefix-fanout=2.9
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTCGTATGCC -o SRR5030863 -
Input file:	STDIN
trimmed:	SRR5030863-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCCGAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:31:53 2020 >> started

Wed Mar  4 06:31:57 2020 >> done (4.298s)
3135404 reads processed; of these:
 153332 ( 4.89%) short reads filtered out after trimming by size control
   9289 ( 0.30%) empty reads filtered out after trimming by size control
2972783 (94.81%) reads available; of these:
2549205 (85.75%) trimmed reads available after processing
 423578 (14.25%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15220	  0.51%
 19	  28285	  0.95%
 20	 165084	  5.55%
 21	 355966	 11.97%
 22	 837477	 28.17%
 23	 365611	 12.30%
 24	 181452	  6.10%
 25	  46971	  1.58%
 26	  24044	  0.81%
 27	  18126	  0.61%
 28	  19293	  0.65%
 29	  13732	  0.46%
 30	  20628	  0.69%
 31	  25401	  0.85%
 32	  28403	  0.96%
 33	  58831	  1.98%
 34	  21843	  0.73%
 35	  16601	  0.56%
 36	  17621	  0.59%
 37	  15718	  0.53%
 38	  16250	  0.55%
 39	  19060	  0.64%
 40	  28586	  0.96%
 41	  40836	  1.37%
 42	  50643	  1.70%
 43	  35204	  1.18%
 44	  29192	  0.98%
 45	  38305	  1.29%
 46	  30218	  1.02%
 47	  22432	  0.75%
 48	   5456	  0.18%
 49	  14450	  0.49%
 50	 365844	 12.31%


criterion=sequence-density
sequence-density=4.97
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=18
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=34
fanout-score=37.07
fanout-score-rank=1
prefix-density=1.11
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:32:23
                             Started mapping on |	Mar 04 06:32:23
                                    Finished on |	Mar 04 06:32:50
       Mapping speed, Million of reads per hour |	407.67

                          Number of input reads |	3057524
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1796512
                        Uniquely mapped reads % |	58.76%
                          Average mapped length |	28.35
                       Number of splices: Total |	1331
            Number of splices: Annotated (sjdb) |	253
                       Number of splices: GT/AG |	1200
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	113
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.48
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	892238
             % of reads mapped to multiple loci |	29.18%
        Number of reads mapped to too many loci |	59033
             % of reads mapped to too many loci |	1.93%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.55%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	368774	368774	368774
N_multimapping	892238	892238	892238
N_noFeature	82182	105545	1282255
N_ambiguous	983523	544479	23768
UnstrandedReadsAssigned:730807 PositiveStrandReadsAssigned:1146488 NegativeStrandReadsAssigned:490489
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030863 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030863-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,057,524 reads, 797,150 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,082 rounds

 109283 SRR5030863.ke.tsv
  52636 SRR5030863.se.tsv
 161919 total
==> SRR5030863.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030863.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030863 completed mapping pipeline successfully
