Starting /dee2/code/volunteer_pipeline.sh SRR5030843
    current disk space = 160679264256
    free memory = 73949652 
SRR5030843 SRAfilesize
5714e119efe308a56f5a12c5f2819d54  SRR5030843.sra
SRR5030843.sra file validated
SRR5030843 is single end
SRR5030843 is conventional basespace
SRR5030843 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5045	34.0	31.0	34.0	31.0	34.0
2	32.71875	34.0	31.0	34.0	31.0	34.0
3	32.83575	34.0	31.0	34.0	31.0	34.0
4	36.28075	37.0	37.0	37.0	35.0	37.0
5	36.218	37.0	37.0	37.0	35.0	37.0
6	36.1905	37.0	36.0	37.0	35.0	37.0
7	36.11675	37.0	35.0	37.0	35.0	37.0
8	36.223	37.0	36.0	37.0	35.0	37.0
9	37.99175	39.0	38.0	39.0	35.0	39.0
10	38.00375	39.0	38.0	39.0	35.0	39.0
11	37.866	39.0	38.0	39.0	35.0	39.0
12	37.86925	39.0	38.0	39.0	35.0	39.0
13	37.824	39.0	38.0	39.0	35.0	39.0
14	39.305	41.0	39.0	41.0	36.0	41.0
15	39.1955	41.0	39.0	41.0	36.0	41.0
16	39.15675	40.0	39.0	41.0	36.0	41.0
17	39.369	41.0	39.0	41.0	36.0	41.0
18	39.2845	41.0	39.0	41.0	36.0	41.0
19	39.12675	41.0	39.0	41.0	36.0	41.0
20	39.1605	41.0	39.0	41.0	36.0	41.0
21	39.26225	41.0	39.0	41.0	36.0	41.0
22	39.24825	41.0	39.0	41.0	36.0	41.0
23	39.01	41.0	39.0	41.0	35.0	41.0
24	38.80275	40.0	38.0	41.0	35.0	41.0
25	39.0375	41.0	39.0	41.0	35.0	41.0
26	39.083	41.0	39.0	41.0	35.0	41.0
27	39.17075	41.0	39.0	41.0	36.0	41.0
28	38.947	41.0	39.0	41.0	35.0	41.0
29	38.377	40.0	38.0	41.0	34.0	41.0
30	38.69575	40.0	38.0	41.0	34.0	41.0
31	38.98075	41.0	39.0	41.0	35.0	41.0
32	39.19775	41.0	39.0	41.0	36.0	41.0
33	38.25525	40.0	38.0	41.0	33.0	41.0
34	38.23825	40.0	38.0	41.0	33.0	41.0
35	37.94475	40.0	38.0	41.0	33.0	41.0
36	34.09875	38.0	30.0	40.0	23.0	41.0
37	35.59025	38.0	34.0	40.0	30.0	41.0
38	37.02525	39.0	37.0	40.0	31.0	41.0
39	36.904	39.0	37.0	40.0	31.0	41.0
40	36.9105	39.0	37.0	41.0	31.0	41.0
41	37.069	39.0	37.0	41.0	31.0	41.0
42	37.39475	40.0	37.0	41.0	33.0	41.0
43	37.225	40.0	37.0	41.0	31.0	41.0
44	37.13	40.0	37.0	41.0	31.0	41.0
45	36.85475	40.0	37.0	41.0	31.0	41.0
46	35.05675	38.0	34.0	40.0	26.0	41.0
47	35.774	39.0	35.0	40.0	28.0	41.0
48	36.1485	39.0	36.0	41.0	30.0	41.0
49	35.191	39.0	35.0	41.0	28.0	41.0
50	30.64875	34.0	27.0	39.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	3.0
17	2.0
18	4.0
19	1.0
20	2.0
21	10.0
22	7.0
23	9.0
24	7.0
25	15.0
26	24.0
27	23.0
28	22.0
29	34.0
30	71.0
31	65.0
32	81.0
33	109.0
34	140.0
35	192.0
36	300.0
37	454.0
38	949.0
39	1474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.131098323742806	23.267450587940957	55.416562421816366	13.184888666499875
2	21.2	25.575	18.875	34.35
3	25.0	19.775000000000002	22.325	32.9
4	28.799999999999997	28.825	13.125	29.25
5	18.2	44.65	25.75	11.4
6	37.375	17.2	33.45	11.975
7	14.249999999999998	20.974999999999998	26.075	38.7
8	25.174999999999997	31.1	13.700000000000001	30.025000000000002
9	22.1	35.775	29.025000000000002	13.100000000000001
10	20.5	22.575	30.65	26.275
11	25.974999999999998	11.5	17.45	45.074999999999996
12	12.75	17.5	32.125	37.625
13	31.75	20.724999999999998	28.499999999999996	19.025
14	30.875000000000004	33.4	20.525	15.2
15	34.492246123061534	26.28814407203602	26.388194097048522	12.831415707853926
16	17.299999999999997	24.95	17.775	39.975
17	20.275000000000002	22.075	43.3	14.35
18	22.95	19.625	30.9	26.525
19	39.825	12.925	29.349999999999998	17.9
20	41.375	15.2	29.599999999999998	13.825000000000001
21	23.25	17.75	29.275000000000002	29.725
22	28.9	12.174999999999999	49.875	9.049999999999999
23	28.775000000000002	11.725	44.55	14.95
24	52.949999999999996	15.1	24.55	7.3999999999999995
25	50.0	26.275	14.475	9.25
26	26.525	49.825	13.750000000000002	9.9
27	14.899999999999999	49.45	25.45	10.2
28	10.4	25.1	50.425	14.075
29	9.049999999999999	13.325000000000001	58.5	19.125
30	8.5	7.049999999999999	37.85	46.6
31	13.425	5.775	48.05	32.75
32	28.175	6.3	19.5	46.025
33	56.75	7.6499999999999995	13.450000000000001	22.15
34	63.74999999999999	6.9750000000000005	15.25	14.025000000000002
35	65.325	6.325	19.625	8.725
36	45.074999999999996	6.625	34.525	13.775
37	45.824999999999996	9.3	19.45	25.424999999999997
38	21.4	15.975	12.075	50.55
39	15.45	26.25	8.35	49.95
40	14.224999999999998	50.975	10.549999999999999	24.25
41	26.150000000000002	50.949999999999996	8.025	14.875
42	51.075	31.125000000000004	10.424999999999999	7.375
43	50.1	33.575	7.95	8.375
44	27.775	53.075	6.05	13.100000000000001
45	16.35	52.05	13.3	18.3
46	8.975	28.075	20.175	42.775
47	6.825	16.725	39.925	36.525
48	8.9	12.7	20.525	57.875
49	13.025	20.974999999999998	14.625	51.37500000000001
50	18.8	37.6	13.225000000000001	30.375000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.5
24	1.0
25	1.5
26	2.0
27	1.0
28	0.0
29	1.0
30	2.0
31	12.0
32	22.0
33	19.0
34	16.0
35	26.0
36	36.0
37	38.5
38	41.0
39	41.5
40	42.0
41	43.0
42	44.0
43	83.5
44	123.0
45	193.0
46	263.0
47	384.5
48	506.0
49	598.0
50	690.0
51	769.5
52	849.0
53	665.5
54	482.0
55	464.0
56	446.0
57	332.5
58	219.0
59	131.0
60	43.0
61	34.0
62	25.0
63	56.0
64	87.0
65	53.0
66	19.0
67	17.5
68	16.0
69	11.0
70	6.0
71	6.5
72	7.0
73	6.0
74	5.0
75	2.5
76	0.0
77	0.5
78	1.0
79	2.0
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.05
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43046357615894	28.475
2	9.470198675496688	7.1499999999999995
3	4.039735099337748	4.575
4	2.384105960264901	3.5999999999999996
5	1.390728476821192	2.625
6	1.3245033112582782	3.0
7	0.7947019867549668	2.1
8	0.9271523178807948	2.8000000000000003
9	0.4635761589403974	1.575
>10	3.1788079470198674	23.075000000000003
>50	0.3973509933774834	11.075
>100	0.1986754966887417	9.950000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	142	3.55	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	141	3.5249999999999995	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	96	2.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	94	2.35	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	69	1.725	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	52	1.3	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	50	1.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	48	1.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	38	0.95	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTC	33	0.8250000000000001	RNA PCR Primer, Index 44 (100% over 44bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGC	19	0.475	RNA PCR Primer, Index 44 (100% over 50bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	18	0.44999999999999996	RNA PCR Primer, Index 7 (97% over 35bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	11	0.27499999999999997	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	10	0.25	RNA PCR Primer, Index 1 (100% over 24bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	10	0.25	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	9	0.22499999999999998	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	9	0.22499999999999998	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	9	0.22499999999999998	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTAT	7	0.17500000000000002	RNA PCR Primer, Index 44 (100% over 48bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	6	0.15	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	6	0.15	No Hit
GCTCGACTCATGGTTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	6	0.15	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	6	0.15	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	5	0.125	No Hit
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TTTTTCATTATTGCTCCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.525	0.0	0.0	0.0
2	0.0	0.55	0.0	0.0	0.0
3	0.0	0.8	0.0	0.0	0.0
4	0.0	0.875	0.0	0.0	0.0
5	0.0	0.9	0.0	0.0	0.0
6	0.0	1.0	0.0	0.0	0.0
7	0.0	1.875	0.0	0.0	0.0
8	0.0	2.1	0.0	0.0	0.0
9	0.0	2.225	0.0	0.0	0.0
10	0.0	2.375	0.0	0.0	0.0
11	0.0	2.55	0.0	0.0	0.0
12	0.0	2.85	0.0	0.0	0.0
13	0.0	3.175	0.0	0.0	0.0
14	0.0	3.475	0.0	0.0	0.0
15	0.0	3.775	0.0	0.0	0.0
16	0.0	4.675	0.0	0.0	0.0
17	0.0	5.225	0.0	0.0	0.0
18	0.0	6.075	0.0	0.0	0.0
19	0.0	6.4	0.0	0.0	0.0
20	0.0	7.475	0.0	0.0	0.0
21	0.0	13.65	0.0	0.0	0.0
22	0.0	26.925	0.0	0.0	0.0
23	0.0	58.525	0.0	0.0	0.0
24	0.0	71.275	0.0	0.0	0.0
25	0.0	77.875	0.0	0.0	0.0
26	0.0	79.1	0.0	0.0	0.0
27	0.0	79.95	0.0	0.0	0.0
28	0.0	80.5	0.0	0.0	0.0
29	0.0	81.05	0.0	0.0	0.0
30	0.0	81.275	0.0	0.0	0.0
31	0.0	81.625	0.0	0.0	0.0
32	0.0	82.1	0.0	0.0	0.0
33	0.0	82.8	0.0	0.0	0.0
34	0.0	84.875	0.0	0.0	0.0
35	0.0	85.55	0.0	0.0	0.0
36	0.0	86.05	0.0	0.0	0.0
37	0.0	86.75	0.0	0.0	0.0
38	0.0	87.075	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGC	20	6.952051E-4	44.000004	32
CATTCAA	20	6.952051E-4	44.000004	3
CTTGGTG	20	6.952051E-4	44.000004	16
ACATTCA	20	6.952051E-4	44.000004	2
GGCTTGG	40	5.315087E-9	44.000004	19
GGCCAAG	20	6.952051E-4	44.000004	36
GCTTGGT	20	6.952051E-4	44.000004	15
GCTTGGA	40	5.315087E-9	44.000004	20
CTCGGGG	20	6.952051E-4	44.000004	30
TTGGTGG	20	6.952051E-4	44.000004	17
GGGGCCA	20	6.952051E-4	44.000004	34
TCTTTTG	20	6.952051E-4	44.000004	17
ATTCAAC	20	6.952051E-4	44.000004	4
AACATTC	20	6.952051E-4	44.000004	1
TCGGGGG	20	6.952051E-4	44.000004	31
GGGCCAA	20	6.952051E-4	44.000004	35
ACAGTGA	35	1.02281774E-7	44.0	3
ACCGGTC	35	1.02281774E-7	44.0	10
AAGTAAT	25	3.6958358E-5	44.0	4
TCAAGTA	25	3.6958358E-5	44.0	2
>>END_MODULE
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261735 spots for SRR5030843.sra
Written 261735 spots for SRR5030843.sra
Read 261737 spots for SRR5030843.sra
Written 261737 spots for SRR5030843.sra
SRR ids: ['SRR5030843.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_v8wmu75f
SRR5030843.sra spots: 3140822
blocks: [[1, 261735], [261736, 523470], [523471, 785205], [785206, 1046940], [1046941, 1308675], [1308676, 1570410], [1570411, 1832145], [1832146, 2093880], [2093881, 2355615], [2355616, 2617350], [2617351, 2879085], [2879086, 3140822]]
SRR5030843 file size 543300
SRR5030843 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030843 SRR5030843_1.fastq
Input file:	SRR5030843_1.fastq
trimmed:	SRR5030843-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:15 2020 >> started

Wed Mar  4 06:26:17 2020 >> done (1.777s)
3140822 reads processed; of these:
    122 ( 0.00%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
3140697 (100.00%) reads available; of these:
 169759 ( 5.41%) trimmed reads available after processing
2970938 (94.59%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     37	  0.00%
 19	     73	  0.00%
 20	    129	  0.00%
 21	    141	  0.00%
 22	    297	  0.01%
 23	    357	  0.01%
 24	    358	  0.01%
 25	    435	  0.01%
 26	    366	  0.01%
 27	    535	  0.02%
 28	    693	  0.02%
 29	   1040	  0.03%
 30	    759	  0.02%
 31	   1007	  0.03%
 32	    944	  0.03%
 33	   1376	  0.04%
 34	   2951	  0.09%
 35	   2848	  0.09%
 36	   2111	  0.07%
 37	   2326	  0.07%
 38	   2410	  0.08%
 39	   2469	  0.08%
 40	   2757	  0.09%
 41	   3042	  0.10%
 42	   3982	  0.13%
 43	   5376	  0.17%
 44	  10244	  0.33%
 45	  11908	  0.38%
 46	  10808	  0.34%
 47	  15689	  0.50%
 48	  34674	  1.10%
 49	  47617	  1.52%
 50	2970938	 94.59%
3140697 reads passed initial QC


criterion=sequence-density
sequence-density=86.29
sequence-density-rank=1
fanout-score=18.56
fanout-score-rank=1
prefix-density=87.55
prefix-fanout=18.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC


criterion=fanout-score
sequence-density=86.29
sequence-density-rank=1
fanout-score=18.56
fanout-score-rank=1
prefix-density=87.55
prefix-fanout=18.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC -o SRR5030843 -
Input file:	STDIN
trimmed:	SRR5030843-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTATAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:33 2020 >> started

Wed Mar  4 06:26:38 2020 >> done (4.907s)
3068497 reads processed; of these:
 177567 ( 5.79%) short reads filtered out after trimming by size control
  15411 ( 0.50%) empty reads filtered out after trimming by size control
2875519 (93.71%) reads available; of these:
2663321 (92.62%) trimmed reads available after processing
 212198 ( 7.38%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  17361	  0.60%
 19	  30564	  1.06%
 20	 197299	  6.86%
 21	 415094	 14.44%
 22	 965784	 33.59%
 23	 371494	 12.92%
 24	 193186	  6.72%
 25	  47865	  1.66%
 26	  22643	  0.79%
 27	  16565	  0.58%
 28	  17167	  0.60%
 29	  12124	  0.42%
 30	  17482	  0.61%
 31	  21504	  0.75%
 32	  21629	  0.75%
 33	  60976	  2.12%
 34	  22876	  0.80%
 35	  18809	  0.65%
 36	  20917	  0.73%
 37	  11402	  0.40%
 38	  11913	  0.41%
 39	  12974	  0.45%
 40	  18593	  0.65%
 41	  24542	  0.85%
 42	  29279	  1.02%
 43	  19450	  0.68%
 44	  17066	  0.59%
 45	  19618	  0.68%
 46	  13598	  0.47%
 47	  10243	  0.36%
 48	   2276	  0.08%
 49	   7406	  0.26%
 50	 185820	  6.46%


criterion=sequence-density
sequence-density=4.66
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AAGCTGCCAGTTGAAGAACTGT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=37
fanout-score=56.31
fanout-score-rank=1
prefix-density=1.05
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:26:59
                             Started mapping on |	Mar 04 06:26:59
                                    Finished on |	Mar 04 06:27:25
       Mapping speed, Million of reads per hour |	408.15

                          Number of input reads |	2947719
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1764858
                        Uniquely mapped reads % |	59.87%
                          Average mapped length |	25.54
                       Number of splices: Total |	1018
            Number of splices: Annotated (sjdb) |	181
                       Number of splices: GT/AG |	945
                       Number of splices: GC/AG |	9
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	64
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	922455
             % of reads mapped to multiple loci |	31.29%
        Number of reads mapped to too many loci |	46495
             % of reads mapped to too many loci |	1.58%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.83%
                     % of reads unmapped: other |	0.43%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	260406	260406	260406
N_multimapping	922455	922455	922455
N_noFeature	58395	72902	1213561
N_ambiguous	1003628	526791	15020
UnstrandedReadsAssigned:702835 PositiveStrandReadsAssigned:1165165 NegativeStrandReadsAssigned:536277
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030843 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030843-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,947,719 reads, 850,060 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,142 rounds

 109283 SRR5030843.ke.tsv
  52636 SRR5030843.se.tsv
 161919 total
==> SRR5030843.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030843.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030843 completed mapping pipeline successfully
