Starting /dee2/code/volunteer_pipeline.sh SRR5030835
    current disk space = 160671776768
    free memory = 73944228 
SRR5030835 SRAfilesize
4576d9b5e5e2a2711621ec6e04d01f4d  SRR5030835.sra
SRR5030835.sra file validated
SRR5030835 is single end
SRR5030835 is conventional basespace
SRR5030835 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.453	34.0	31.0	34.0	31.0	34.0
2	32.7425	34.0	31.0	34.0	31.0	34.0
3	32.86975	34.0	31.0	34.0	31.0	34.0
4	36.28725	37.0	37.0	37.0	35.0	37.0
5	36.32125	37.0	37.0	37.0	35.0	37.0
6	36.08675	37.0	36.0	37.0	35.0	37.0
7	36.021	37.0	35.0	37.0	35.0	37.0
8	36.178	37.0	36.0	37.0	35.0	37.0
9	37.89	39.0	38.0	39.0	35.0	39.0
10	37.959	39.0	38.0	39.0	35.0	39.0
11	37.85	39.0	38.0	39.0	35.0	39.0
12	37.81025	39.0	38.0	39.0	35.0	39.0
13	37.7725	39.0	38.0	39.0	35.0	39.0
14	39.1905	41.0	39.0	41.0	36.0	41.0
15	39.121	40.0	38.0	41.0	36.0	41.0
16	39.1025	40.0	39.0	41.0	36.0	41.0
17	39.226	41.0	39.0	41.0	36.0	41.0
18	39.292	41.0	39.0	41.0	36.0	41.0
19	39.126	41.0	39.0	41.0	36.0	41.0
20	39.07025	40.0	39.0	41.0	35.0	41.0
21	39.176	40.0	39.0	41.0	36.0	41.0
22	39.1695	41.0	39.0	41.0	36.0	41.0
23	38.878	40.0	39.0	41.0	35.0	41.0
24	38.59925	40.0	38.0	41.0	34.0	41.0
25	38.992	41.0	39.0	41.0	35.0	41.0
26	39.124	41.0	39.0	41.0	35.0	41.0
27	39.16275	41.0	39.0	41.0	36.0	41.0
28	38.824	40.0	38.0	41.0	35.0	41.0
29	38.09575	40.0	38.0	41.0	33.0	41.0
30	38.375	40.0	38.0	41.0	34.0	41.0
31	38.568	40.0	38.0	41.0	34.0	41.0
32	38.928	40.0	39.0	41.0	35.0	41.0
33	38.03975	40.0	38.0	41.0	33.0	41.0
34	38.05275	40.0	38.0	41.0	33.0	41.0
35	37.93	40.0	37.0	41.0	33.0	41.0
36	33.9815	38.0	30.0	40.0	23.0	41.0
37	35.35975	38.0	34.0	40.0	27.0	41.0
38	36.98525	39.0	37.0	40.0	31.0	41.0
39	37.0145	39.0	37.0	40.0	31.0	41.0
40	37.0205	39.0	37.0	41.0	31.0	41.0
41	36.9785	39.0	36.0	41.0	31.0	41.0
42	37.1695	40.0	37.0	41.0	31.0	41.0
43	37.10425	39.0	37.0	41.0	31.0	41.0
44	36.89275	39.0	36.0	41.0	31.0	41.0
45	36.61975	39.0	36.0	40.0	30.0	41.0
46	35.044	38.0	34.0	40.0	26.0	41.0
47	35.582	39.0	35.0	40.0	28.0	41.0
48	35.88125	39.0	35.0	41.0	29.0	41.0
49	34.68925	38.0	34.0	40.0	26.0	41.0
50	30.1	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	1.0
19	2.0
20	1.0
21	2.0
22	8.0
23	7.0
24	12.0
25	18.0
26	23.0
27	19.0
28	30.0
29	44.0
30	71.0
31	66.0
32	83.0
33	125.0
34	169.0
35	196.0
36	315.0
37	524.0
38	979.0
39	1301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.587881822734102	24.061091637456183	54.406609914872305	12.944416624937405
2	22.5	25.8	18.75	32.95
3	26.5	21.45	20.9	31.15
4	31.674999999999997	28.199999999999996	13.375	26.75
5	21.224999999999998	43.75	24.825	10.2
6	36.199999999999996	15.65	35.125	13.025
7	16.025	21.25	25.674999999999997	37.05
8	26.674999999999997	30.0	13.375	29.95
9	21.5	35.875	28.299999999999997	14.325
10	21.85	21.55	30.675	25.924999999999997
11	28.7	11.05	16.1	44.15
12	15.35	18.025	31.5	35.125
13	31.825	21.7	29.049999999999997	17.424999999999997
14	30.925000000000004	32.725	21.349999999999998	15.0
15	35.425000000000004	25.275	26.0	13.3
16	16.425	23.925	20.474999999999998	39.175
17	21.099999999999998	21.5	43.5	13.900000000000002
18	23.150000000000002	18.95	31.525	26.375
19	37.65	14.149999999999999	29.299999999999997	18.9
20	43.3	15.45	28.599999999999998	12.65
21	22.975	18.625	29.25	29.15
22	27.425	13.25	49.4	9.925
23	27.075	13.4	45.025	14.499999999999998
24	51.4	15.5	24.525	8.575000000000001
25	50.5	24.45	14.825	10.225
26	26.85	47.5	15.049999999999999	10.6
27	15.375	48.25	24.7	11.675
28	11.65	24.875	49.575	13.900000000000002
29	10.6	12.75	57.75	18.9
30	10.299999999999999	6.75	36.0	46.949999999999996
31	14.725	6.175	46.225	32.875
32	27.950000000000003	6.6000000000000005	20.825	44.625
33	55.675	8.125	13.625000000000002	22.575
34	61.75000000000001	7.1499999999999995	15.825	15.275
35	63.075	6.9	18.575	11.450000000000001
36	42.725	8.1	34.475	14.7
37	46.175	9.725	19.475	24.625
38	23.474999999999998	15.525	12.1	48.9
39	16.6	24.425	8.9	50.075
40	15.950000000000001	48.975	10.6	24.474999999999998
41	25.174999999999997	50.025	8.875	15.925
42	50.975	30.4	9.35	9.275
43	51.1	31.1	8.674999999999999	9.125
44	28.15	50.849999999999994	6.950000000000001	14.05
45	16.975	51.975	13.575000000000001	17.474999999999998
46	11.125	26.775	18.5	43.6
47	8.425	15.75	39.800000000000004	36.025
48	9.625	14.099999999999998	19.400000000000002	56.875
49	13.575000000000001	20.375	13.825000000000001	52.225
50	18.5	38.074999999999996	13.325000000000001	30.099999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.0
28	1.0
29	1.0
30	1.0
31	10.0
32	19.0
33	19.5
34	20.0
35	27.0
36	34.0
37	48.5
38	63.0
39	55.5
40	48.0
41	50.5
42	53.0
43	88.0
44	123.0
45	162.0
46	201.0
47	328.5
48	456.0
49	582.0
50	708.0
51	764.0
52	820.0
53	619.5
54	419.0
55	455.0
56	491.0
57	364.5
58	238.0
59	153.0
60	68.0
61	60.0
62	52.0
63	65.0
64	78.0
65	54.0
66	30.0
67	24.5
68	19.0
69	15.5
70	12.0
71	21.0
72	30.0
73	17.0
74	4.0
75	3.0
76	2.0
77	1.5
78	1.0
79	4.0
80	7.0
81	3.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82070707070707	30.025000000000002
2	9.722222222222223	7.7
3	3.5984848484848486	4.275
4	2.083333333333333	3.3000000000000003
5	1.9570707070707072	3.875
6	1.073232323232323	2.55
7	1.073232323232323	2.9749999999999996
8	0.6944444444444444	2.1999999999999997
9	0.25252525252525254	0.8999999999999999
>10	3.2196969696969697	23.325000000000003
>50	0.31565656565656564	9.75
>100	0.1893939393939394	9.125
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	136	3.4000000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	126	3.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	98	2.45	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	86	2.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	63	1.575	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	39	0.975	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	34	0.8500000000000001	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	20	0.5	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	19	0.475	RNA PCR Primer, Index 7 (100% over 35bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC	18	0.44999999999999996	RNA PCR Primer, Index 31 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC	15	0.375	RNA PCR Primer, Index 31 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	13	0.325	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	13	0.325	No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	11	0.27499999999999997	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	11	0.27499999999999997	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	10	0.25	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	10	0.25	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	8	0.2	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	8	0.2	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	7	0.17500000000000002	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	7	0.17500000000000002	No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	7	0.17500000000000002	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTAT	7	0.17500000000000002	RNA PCR Primer, Index 31 (100% over 48bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	6	0.15	RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	6	0.15	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	6	0.15	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG	6	0.15	RNA PCR Primer, Index 31 (100% over 37bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
ATACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTCCCGGGCGGGC	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.375	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.575	0.0	0.0	0.0
4	0.0	0.575	0.0	0.0	0.0
5	0.0	0.6	0.0	0.0	0.0
6	0.0	0.7	0.0	0.0	0.0
7	0.0	1.2	0.0	0.0	0.0
8	0.0	1.3	0.0	0.0	0.0
9	0.0	1.375	0.0	0.0	0.0
10	0.0	1.575	0.0	0.0	0.0
11	0.0	1.75	0.0	0.0	0.0
12	0.0	1.85	0.0	0.0	0.0
13	0.0	1.95	0.0	0.0	0.0
14	0.0	2.425	0.0	0.0	0.0
15	0.0	2.75	0.0	0.0	0.0
16	0.0	3.675	0.0	0.0	0.0
17	0.0	4.2	0.0	0.0	0.0
18	0.0	5.3	0.0	0.0	0.0
19	0.0	5.725	0.0	0.0	0.0
20	0.0	6.75	0.0	0.0	0.0
21	0.0	12.8	0.0	0.0	0.0
22	0.0	24.25	0.0	0.0	0.0
23	0.0	54.625	0.0	0.0	0.0
24	0.0	66.975	0.0	0.0	0.0
25	0.0	73.05	0.0	0.0	0.0
26	0.0	74.5	0.0	0.0	0.0
27	0.0	75.4	0.0	0.0	0.0
28	0.0	76.05	0.0	0.0	0.0
29	0.0	76.525	0.0	0.0	0.0
30	0.0	76.9	0.0	0.0	0.0
31	0.0	77.325	0.0	0.0	0.0
32	0.0	78.325	0.0	0.0	0.0
33	0.0	78.9	0.0	0.0	0.0
34	0.0	80.55	0.0	0.0	0.0
35	0.0	81.125	0.0	0.0	0.0
36	0.0	81.55	0.0	0.0	0.0
37	0.0	82.375	0.0	0.0	0.0
38	0.0	82.8	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
CGCGACC	20	6.952051E-4	44.000004	1
CGCTGAT	20	6.952051E-4	44.000004	28
AATCCAG	20	6.952051E-4	44.000004	8
CTGAGAC	20	6.952051E-4	44.000004	4
ATCCAGG	20	6.952051E-4	44.000004	9
AGTAGGT	20	6.952051E-4	44.000004	7
GCGACCT	20	6.952051E-4	44.000004	2
CCGCTGA	20	6.952051E-4	44.000004	27
ATAGGCT	20	6.952051E-4	44.000004	16
GAGCTTG	40	5.315087E-9	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
CCTGAGA	20	6.952051E-4	44.000004	3
TCGGATC	40	5.315087E-9	44.000004	1
ACCCGCT	20	6.952051E-4	44.000004	25
CGGATCC	40	5.315087E-9	44.000004	2
TCCAGGA	20	6.952051E-4	44.000004	10
GTGAGTT	20	6.952051E-4	44.000004	18
CCCGCTG	20	6.952051E-4	44.000004	26
>>END_MODULE
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177932 spots for SRR5030835.sra
Written 177932 spots for SRR5030835.sra
Read 177933 spots for SRR5030835.sra
Written 177933 spots for SRR5030835.sra
SRR ids: ['SRR5030835.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_useo0ldo
SRR5030835.sra spots: 2135185
blocks: [[1, 177932], [177933, 355864], [355865, 533796], [533797, 711728], [711729, 889660], [889661, 1067592], [1067593, 1245524], [1245525, 1423456], [1423457, 1601388], [1601389, 1779320], [1779321, 1957252], [1957253, 2135185]]
SRR5030835 file size 368998
SRR5030835 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030835 SRR5030835_1.fastq
Input file:	SRR5030835_1.fastq
trimmed:	SRR5030835-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:23:51 2020 >> started

Wed Mar  4 06:23:52 2020 >> done (1.429s)
2135185 reads processed; of these:
    102 ( 0.00%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2135077 (99.99%) reads available; of these:
 120700 ( 5.65%) trimmed reads available after processing
2014377 (94.35%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     31	  0.00%
 19	     39	  0.00%
 20	     98	  0.00%
 21	    117	  0.01%
 22	    204	  0.01%
 23	    223	  0.01%
 24	    246	  0.01%
 25	    304	  0.01%
 26	    272	  0.01%
 27	    367	  0.02%
 28	    474	  0.02%
 29	    658	  0.03%
 30	    467	  0.02%
 31	    598	  0.03%
 32	    579	  0.03%
 33	    905	  0.04%
 34	   1765	  0.08%
 35	   1782	  0.08%
 36	   1447	  0.07%
 37	   1572	  0.07%
 38	   1807	  0.08%
 39	   1816	  0.09%
 40	   2054	  0.10%
 41	   2346	  0.11%
 42	   2867	  0.13%
 43	   4087	  0.19%
 44	   7207	  0.34%
 45	   8170	  0.38%
 46	   7989	  0.37%
 47	  11418	  0.53%
 48	  26084	  1.22%
 49	  32707	  1.53%
 50	2014377	 94.35%
2135077 reads passed initial QC


criterion=sequence-density
sequence-density=83.43
sequence-density-rank=1
fanout-score=19.79
fanout-score-rank=1
prefix-density=84.82
prefix-fanout=19.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC


criterion=fanout-score
sequence-density=83.43
sequence-density-rank=1
fanout-score=19.79
fanout-score-rank=1
prefix-density=84.82
prefix-fanout=19.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC -o SRR5030835 -
Input file:	STDIN
trimmed:	SRR5030835-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:24:07 2020 >> started

Wed Mar  4 06:24:10 2020 >> done (2.817s)
2084242 reads processed; of these:
 112632 ( 5.40%) short reads filtered out after trimming by size control
   9378 ( 0.45%) empty reads filtered out after trimming by size control
1962232 (94.15%) reads available; of these:
1776697 (90.54%) trimmed reads available after processing
 185535 ( 9.46%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12366	  0.63%
 19	  19782	  1.01%
 20	 114088	  5.81%
 21	 259876	 13.24%
 22	 628292	 32.02%
 23	 268391	 13.68%
 24	 128738	  6.56%
 25	  32876	  1.68%
 26	  15925	  0.81%
 27	  11709	  0.60%
 28	  12001	  0.61%
 29	   8835	  0.45%
 30	  13897	  0.71%
 31	  21579	  1.10%
 32	  16087	  0.82%
 33	  40188	  2.05%
 34	  14326	  0.73%
 35	  12023	  0.61%
 36	  13853	  0.71%
 37	   8306	  0.42%
 38	   8338	  0.42%
 39	   8793	  0.45%
 40	  13358	  0.68%
 41	  18577	  0.95%
 42	  22204	  1.13%
 43	  15738	  0.80%
 44	  12666	  0.65%
 45	  15990	  0.81%
 46	  11493	  0.59%
 47	   9380	  0.48%
 48	   1919	  0.10%
 49	   5950	  0.30%
 50	 164688	  8.39%


criterion=sequence-density
sequence-density=5.37
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=18
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=37
fanout-score=170.51
fanout-score-rank=1
prefix-density=1.91
prefix-fanout=1.9
sequence=TTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:24:34
                             Started mapping on |	Mar 04 06:24:35
                                    Finished on |	Mar 04 06:25:03
       Mapping speed, Million of reads per hour |	258.82

                          Number of input reads |	2013067
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1162285
                        Uniquely mapped reads % |	57.74%
                          Average mapped length |	26.17
                       Number of splices: Total |	858
            Number of splices: Annotated (sjdb) |	99
                       Number of splices: GT/AG |	807
                       Number of splices: GC/AG |	5
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	46
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.69
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	637473
             % of reads mapped to multiple loci |	31.67%
        Number of reads mapped to too many loci |	34301
             % of reads mapped to too many loci |	1.70%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.39%
                     % of reads unmapped: other |	0.50%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	213309	213309	213309
N_multimapping	637473	637473	637473
N_noFeature	52003	62961	823365
N_ambiguous	645261	351327	10115
UnstrandedReadsAssigned:465021 PositiveStrandReadsAssigned:747997 NegativeStrandReadsAssigned:328805
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030835 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030835-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,013,067 reads, 527,147 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,078 rounds

 109283 SRR5030835.ke.tsv
  52636 SRR5030835.se.tsv
 161919 total
==> SRR5030835.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030835.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030835 completed mapping pipeline successfully
