Starting /dee2/code/volunteer_pipeline.sh SRR5030836
    current disk space = 722342608
    free memory = 87393072 
SRR5030836 SRAfilesize
a5e773a591611433087e9f5f416fdeb3  SRR5030836.sra
SRR5030836.sra file validated
SRR5030836 is single end
SRR5030836 is conventional basespace
SRR5030836 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34275	33.0	31.0	34.0	31.0	34.0
2	32.6985	34.0	31.0	34.0	31.0	34.0
3	32.75375	34.0	31.0	34.0	31.0	34.0
4	36.258	37.0	37.0	37.0	35.0	37.0
5	36.19525	37.0	37.0	37.0	35.0	37.0
6	36.047	37.0	35.0	37.0	35.0	37.0
7	36.06925	37.0	35.0	37.0	35.0	37.0
8	36.12925	37.0	36.0	37.0	35.0	37.0
9	37.83475	39.0	38.0	39.0	35.0	39.0
10	37.835	39.0	38.0	39.0	35.0	39.0
11	37.8555	39.0	38.0	39.0	35.0	39.0
12	37.8505	39.0	38.0	39.0	35.0	39.0
13	37.8335	39.0	38.0	39.0	35.0	39.0
14	39.10425	41.0	38.0	41.0	36.0	41.0
15	39.05725	40.0	39.0	41.0	36.0	41.0
16	39.0705	40.0	38.0	41.0	35.0	41.0
17	39.151	40.0	39.0	41.0	36.0	41.0
18	39.11825	41.0	38.0	41.0	35.0	41.0
19	38.944	40.0	38.0	41.0	35.0	41.0
20	38.9775	40.0	39.0	41.0	35.0	41.0
21	38.888	40.0	38.0	41.0	35.0	41.0
22	38.61125	40.0	38.0	41.0	34.0	41.0
23	38.49575	40.0	38.0	41.0	34.0	41.0
24	37.845	40.0	38.0	41.0	33.0	41.0
25	38.27175	40.0	38.0	41.0	34.0	41.0
26	38.49875	40.0	38.0	41.0	34.0	41.0
27	38.59275	40.0	38.0	41.0	35.0	41.0
28	38.5375	40.0	38.0	41.0	34.0	41.0
29	38.31075	40.0	38.0	41.0	33.0	41.0
30	38.0675	40.0	38.0	41.0	33.0	41.0
31	38.474	40.0	38.0	41.0	34.0	41.0
32	38.65325	40.0	38.0	41.0	34.0	41.0
33	37.176	40.0	37.0	41.0	31.0	41.0
34	36.40975	40.0	36.0	41.0	29.0	41.0
35	32.6935	38.0	31.0	40.0	15.0	41.0
36	29.29575	37.0	18.0	40.0	8.0	41.0
37	29.89775	35.0	25.0	39.0	6.0	41.0
38	32.37025	37.0	30.0	40.0	15.0	41.0
39	32.18625	37.0	31.0	40.0	9.0	41.0
40	32.77975	38.0	32.0	40.0	8.0	41.0
41	30.35025	36.0	26.0	40.0	2.0	41.0
42	32.09225	37.0	31.0	40.0	2.0	41.0
43	30.333	37.0	26.0	40.0	2.0	41.0
44	31.308	37.0	31.0	40.0	2.0	41.0
45	29.3395	36.0	26.0	40.0	2.0	41.0
46	27.28775	34.0	15.0	39.0	2.0	41.0
47	27.23725	34.0	15.0	39.0	2.0	41.0
48	27.791	35.0	15.0	39.0	2.0	41.0
49	25.893	34.0	2.0	39.0	2.0	41.0
50	22.35425	29.0	2.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	0.0
16	2.0
17	1.0
18	3.0
19	6.0
20	10.0
21	17.0
22	27.0
23	39.0
24	43.0
25	63.0
26	94.0
27	133.0
28	76.0
29	118.0
30	156.0
31	155.0
32	159.0
33	165.0
34	257.0
35	316.0
36	298.0
37	466.0
38	621.0
39	771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.742613920881322	24.461692538808215	50.07511266900351	13.72058087130696
2	24.375	26.0	19.35	30.275000000000002
3	25.924999999999997	21.975	21.125	30.975
4	28.875	27.875	15.375	27.875
5	20.775	40.45	25.874999999999996	12.9
6	34.375	17.474999999999998	34.75	13.4
7	18.375	19.475	27.875	34.275
8	28.125	27.3	14.374999999999998	30.2
9	20.575	35.825	29.049999999999997	14.549999999999999
10	22.925	22.025	28.000000000000004	27.05
11	32.625	13.3	15.950000000000001	38.125
12	19.15	20.025000000000002	28.375	32.45
13	32.775	19.025	30.475	17.724999999999998
14	29.849999999999998	28.4	25.374999999999996	16.375
15	33.650000000000006	22.650000000000002	26.85	16.85
16	19.1	20.25	21.925	38.725
17	22.375	23.525	37.25	16.85
18	22.725	21.099999999999998	29.175	27.0
19	31.724999999999998	16.85	31.974999999999998	19.45
20	39.825	16.075	28.9	15.2
21	26.1	20.1	28.15	25.650000000000002
22	26.05	15.75	45.15	13.05
23	24.675	15.975	44.1	15.25
24	47.525	13.700000000000001	26.424999999999997	12.35
25	48.199999999999996	21.4	18.224999999999998	12.174999999999999
26	27.750000000000004	42.5	14.475	15.275
27	17.625	45.1	22.45	14.825
28	14.499999999999998	25.95	44.5	15.049999999999999
29	13.3	14.549999999999999	53.7	18.45
30	12.325	7.324999999999999	36.075	44.275
31	14.45	7.049999999999999	43.85	34.65
32	25.7	9.1	23.1	42.1
33	51.075	8.975	15.0	24.95
34	58.050000000000004	7.3999999999999995	15.1	19.45
35	59.475	9.049999999999999	18.85	12.625
36	60.62499999999999	9.775	16.25	13.350000000000001
37	39.25	13.950000000000001	21.725	25.074999999999996
38	25.525	16.325	14.149999999999999	44.0
39	18.25	23.549999999999997	12.65	45.550000000000004
40	16.375	45.875	12.0	25.75
41	25.775	42.875	13.725000000000001	17.625
42	47.949999999999996	28.225	12.0	11.825
43	44.05	33.800000000000004	11.275	10.875
44	29.7	46.400000000000006	8.375	15.525
45	21.3	45.6	12.825000000000001	20.275000000000002
46	14.35	28.125	25.3	32.225
47	11.1	18.625	32.45	37.824999999999996
48	12.525	12.55	22.6	52.325
49	16.3	25.1	13.575000000000001	45.025
50	29.575000000000003	25.924999999999997	10.7	33.800000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	2.5
28	4.0
29	3.5
30	3.0
31	4.0
32	5.0
33	11.0
34	17.0
35	34.0
36	51.0
37	61.0
38	71.0
39	66.0
40	61.0
41	68.5
42	76.0
43	91.5
44	107.0
45	156.5
46	206.0
47	250.5
48	295.0
49	406.5
50	518.0
51	708.0
52	898.0
53	707.5
54	517.0
55	431.0
56	345.0
57	335.5
58	326.0
59	237.5
60	149.0
61	117.5
62	86.0
63	111.0
64	136.0
65	86.0
66	36.0
67	25.0
68	14.0
69	15.0
70	16.0
71	25.0
72	34.0
73	20.5
74	7.0
75	5.5
76	4.0
77	2.5
78	1.0
79	3.5
80	6.0
81	5.5
82	5.0
83	2.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74199713330148	41.725
2	9.26899187768753	9.700000000000001
3	3.1533683707596754	4.95
4	1.5766841853798377	3.3000000000000003
5	1.433349259436216	3.75
6	0.8600095556617295	2.7
7	0.6211180124223602	2.275
8	0.5733397037744864	2.4
9	0.3822264691829909	1.7999999999999998
>10	2.2455805064500716	20.424999999999997
>50	0.09555661729574773	4.45
>100	0.047778308647873864	2.5250000000000004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	98	2.45	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC	80	2.0	RNA PCR Primer, Index 37 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	41	1.0250000000000001	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (96% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	25	0.625	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	18	0.44999999999999996	RNA PCR Primer, Index 1 (96% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC	16	0.4	RNA PCR Primer, Index 37 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	15	0.375	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	14	0.35000000000000003	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	13	0.325	RNA PCR Primer, Index 2 (100% over 35bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	13	0.325	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	12	0.3	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	12	0.3	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	10	0.25	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	9	0.22499999999999998	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	9	0.22499999999999998	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	9	0.22499999999999998	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	8	0.2	RNA PCR Primer, Index 1 (96% over 27bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	8	0.2	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCAG	8	0.2	RNA PCR Primer, Index 1 (96% over 26bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	8	0.2	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAT	8	0.2	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	8	0.2	No Hit
GAGCAATGATGACCCCAACATGCCATCTGAGTGGCTTTGCTGAAATCCAG	8	0.2	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAATTCCG	7	0.17500000000000002	RNA PCR Primer, Index 1 (95% over 23bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	7	0.17500000000000002	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GCTGTACTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTG	7	0.17500000000000002	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGGGCCAAGGAACTCCG	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	6	0.15	RNA PCR Primer, Index 2 (100% over 35bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	6	0.15	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	6	0.15	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	6	0.15	RNA PCR Primer, Index 1 (96% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	5	0.125	No Hit
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAT	5	0.125	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	5	0.125	No Hit
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCATGATGACTTGAATTGTCGGATACCCCTTCACCCCGTTCATGGGTG	5	0.125	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG	5	0.125	RNA PCR Primer, Index 7 (97% over 36bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	2.175	0.0	0.0	0.0
2	0.0	2.175	0.0	0.0	0.0
3	0.0	2.25	0.0	0.0	0.0
4	0.0	2.275	0.0	0.0	0.0
5	0.0	2.325	0.0	0.0	0.0
6	0.0	2.4	0.0	0.0	0.0
7	0.0	2.9	0.0	0.0	0.0
8	0.0	3.075	0.0	0.0	0.0
9	0.0	3.1	0.0	0.0	0.0
10	0.0	3.325	0.0	0.0	0.0
11	0.0	3.425	0.0	0.0	0.0
12	0.0	3.6	0.0	0.0	0.0
13	0.0	3.775	0.0	0.0	0.0
14	0.0	4.0	0.0	0.0	0.0
15	0.0	4.4	0.0	0.0	0.0
16	0.0	5.2	0.0	0.0	0.0
17	0.0	5.7	0.0	0.0	0.0
18	0.0	6.55	0.0	0.0	0.0
19	0.0	7.15	0.0	0.0	0.0
20	0.0	7.825	0.0	0.0	0.0
21	0.0	12.25	0.0	0.0	0.0
22	0.0	21.625	0.0	0.0	0.0
23	0.0	47.85	0.0	0.0	0.0
24	0.0	59.75	0.0	0.0	0.0
25	0.0	65.6	0.0	0.0	0.0
26	0.0	67.2	0.0	0.0	0.0
27	0.0	67.95	0.0	0.0	0.0
28	0.0	68.6	0.0	0.0	0.0
29	0.0	68.9	0.0	0.0	0.0
30	0.0	69.25	0.0	0.0	0.0
31	0.0	70.1	0.0	0.0	0.0
32	0.0	71.7	0.0	0.0	0.0
33	0.0	72.475	0.0	0.0	0.0
34	0.0	74.925	0.0	0.0	0.0
35	0.0	76.025	0.0	0.0	0.0
36	0.0	76.65	0.0	0.0	0.0
37	0.0	77.55	0.0	0.0	0.0
38	0.0	78.05	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
ACTGTTG	20	6.952051E-4	44.000004	18
GTTGAAG	20	6.952051E-4	44.000004	10
TTGCACT	20	6.952051E-4	44.000004	3
CAGTTGA	20	6.952051E-4	44.000004	8
CTGAGAC	20	6.952051E-4	44.000004	4
CCAGTTG	20	6.952051E-4	44.000004	7
AACTGTT	20	6.952051E-4	44.000004	17
CCCGGCC	20	6.952051E-4	44.000004	13
CCTGAGA	20	6.952051E-4	44.000004	3
TATTGCA	20	6.952051E-4	44.000004	1
GAAGAAC	20	6.952051E-4	44.000004	13
AGAACTG	20	6.952051E-4	44.000004	15
GCCAGTT	20	6.952051E-4	44.000004	6
GGGGGGG	20	6.952051E-4	44.000004	1
GCTGCCA	20	6.952051E-4	44.000004	3
GAACTGT	20	6.952051E-4	44.000004	16
ATTGCAC	20	6.952051E-4	44.000004	2
TGCCAGT	20	6.952051E-4	44.000004	5
CCCTGAG	20	6.952051E-4	44.000004	2
>>END_MODULE
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
Read 81902 spots for SRR5030836.sra
Written 81902 spots for SRR5030836.sra
Read 81899 spots for SRR5030836.sra
Written 81899 spots for SRR5030836.sra
SRR ids: ['SRR5030836.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xv0u2ea3
SRR5030836.sra spots: 2620771
blocks: [[1, 81899], [81900, 163798], [163799, 245697], [245698, 327596], [327597, 409495], [409496, 491394], [491395, 573293], [573294, 655192], [655193, 737091], [737092, 818990], [818991, 900889], [900890, 982788], [982789, 1064687], [1064688, 1146586], [1146587, 1228485], [1228486, 1310384], [1310385, 1392283], [1392284, 1474182], [1474183, 1556081], [1556082, 1637980], [1637981, 1719879], [1719880, 1801778], [1801779, 1883677], [1883678, 1965576], [1965577, 2047475], [2047476, 2129374], [2129375, 2211273], [2211274, 2293172], [2293173, 2375071], [2375072, 2456970], [2456971, 2538869], [2538870, 2620771]]
SRR5030836 file size 453160
SRR5030836 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 32 -o SRR5030836 SRR5030836_1.fastq
Input file:	SRR5030836_1.fastq
trimmed:	SRR5030836-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Fri Jan 10 18:44:10 2020 >> started

Fri Jan 10 18:44:13 2020 >> done (2.348s)
2620771 reads processed; of these:
    207 ( 0.01%) short reads filtered out after trimming by size control
     11 ( 0.00%) empty reads filtered out after trimming by size control
2620553 (99.99%) reads available; of these:
 311441 (11.88%) trimmed reads available after processing
2309112 (88.12%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     62	  0.00%
 19	     74	  0.00%
 20	    138	  0.01%
 21	    192	  0.01%
 22	    291	  0.01%
 23	    366	  0.01%
 24	    360	  0.01%
 25	    505	  0.02%
 26	    437	  0.02%
 27	    518	  0.02%
 28	    659	  0.03%
 29	   1034	  0.04%
 30	    798	  0.03%
 31	   1066	  0.04%
 32	   2740	  0.10%
 33	   8202	  0.31%
 34	  20154	  0.77%
 35	   4501	  0.17%
 36	   4974	  0.19%
 37	   2945	  0.11%
 38	   5607	  0.21%
 39	   5753	  0.22%
 40	  12045	  0.46%
 41	   6181	  0.24%
 42	  17861	  0.68%
 43	   6615	  0.25%
 44	  27507	  1.05%
 45	  19476	  0.74%
 46	  17478	  0.67%
 47	  19960	  0.76%
 48	  59083	  2.25%
 49	  63859	  2.44%
 50	2309112	 88.12%
2620553 reads passed initial QC


criterion=sequence-density
sequence-density=77.20
sequence-density-rank=1
fanout-score=20.99
fanout-score-rank=3
prefix-density=78.08
prefix-fanout=20.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCCG


criterion=fanout-score
sequence-density=1.41
sequence-density-rank=6
fanout-score=518.06
fanout-score-rank=1
prefix-density=77.40
prefix-fanout=9.5
sequence=GGAATTCTCGGTGCCAAGGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 32 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCCG -o SRR5030836 -
Input file:	STDIN
trimmed:	SRR5030836-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Fri Jan 10 18:44:29 2020 >> started

Fri Jan 10 18:44:33 2020 >> done (3.892s)
2553359 reads processed; of these:
 127875 ( 5.01%) short reads filtered out after trimming by size control
  57043 ( 2.23%) empty reads filtered out after trimming by size control
2368441 (92.76%) reads available; of these:
2014548 (85.06%) trimmed reads available after processing
 353893 (14.94%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13746	  0.58%
 19	  21861	  0.92%
 20	  99368	  4.20%
 21	 261509	 11.04%
 22	 652215	 27.54%
 23	 314469	 13.28%
 24	 156772	  6.62%
 25	  41381	  1.75%
 26	  21260	  0.90%
 27	  16549	  0.70%
 28	  16816	  0.71%
 29	  15130	  0.64%
 30	  20405	  0.86%
 31	  32984	  1.39%
 32	  25795	  1.09%
 33	  59286	  2.50%
 34	  26284	  1.11%
 35	  20848	  0.88%
 36	  30071	  1.27%
 37	  12268	  0.52%
 38	  13723	  0.58%
 39	  13729	  0.58%
 40	  21282	  0.90%
 41	  25092	  1.06%
 42	  32455	  1.37%
 43	  21308	  0.90%
 44	  21700	  0.92%
 45	  22394	  0.95%
 46	  18718	  0.79%
 47	  15263	  0.64%
 48	   6191	  0.26%
 49	  10587	  0.45%
 50	 286982	 12.12%


criterion=sequence-density
sequence-density=5.82
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=433.57
fanout-score-rank=1
prefix-density=2.09
prefix-fanout=1.8
sequence=TTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Jan 10 18:45:05
                             Started mapping on |	Jan 10 18:45:05
                                    Finished on |	Jan 10 18:45:33
       Mapping speed, Million of reads per hour |	313.15

                          Number of input reads |	2435635
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1307546
                        Uniquely mapped reads % |	53.68%
                          Average mapped length |	27.83
                       Number of splices: Total |	1438
            Number of splices: Annotated (sjdb) |	199
                       Number of splices: GT/AG |	1338
                       Number of splices: GC/AG |	24
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	75
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.23
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	769117
             % of reads mapped to multiple loci |	31.58%
        Number of reads mapped to too many loci |	59049
             % of reads mapped to too many loci |	2.42%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.52%
                     % of reads unmapped: other |	0.79%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	358972	358972	358972
N_multimapping	769117	769117	769117
N_noFeature	75929	91287	976670
N_ambiguous	701221	418267	14404
UnstrandedReadsAssigned:530396 PositiveStrandReadsAssigned:797992 NegativeStrandReadsAssigned:316472
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030836 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030836-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,435,635 reads, 588,234 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 871 rounds

 109283 SRR5030836.ke.tsv
  52636 SRR5030836.se.tsv
 161919 total
==> SRR5030836.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030836.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030836 completed mapping pipeline successfully
