Starting /dee2/code/volunteer_pipeline.sh SRR5030837
    current disk space = 160666533888
    free memory = 73949528 
SRR5030837 SRAfilesize
3dcccf6fed9034a28f35ade3498f0498  SRR5030837.sra
SRR5030837.sra file validated
SRR5030837 is single end
SRR5030837 is conventional basespace
SRR5030837 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28925	33.0	31.0	34.0	31.0	34.0
2	32.6515	34.0	31.0	34.0	31.0	34.0
3	32.72625	34.0	31.0	34.0	31.0	34.0
4	36.23375	37.0	37.0	37.0	35.0	37.0
5	36.17575	37.0	37.0	37.0	35.0	37.0
6	35.97275	37.0	35.0	37.0	35.0	37.0
7	36.05725	37.0	35.0	37.0	35.0	37.0
8	36.14675	37.0	35.0	37.0	35.0	37.0
9	37.85375	39.0	38.0	39.0	35.0	39.0
10	37.83825	39.0	38.0	39.0	35.0	39.0
11	37.757	39.0	38.0	39.0	35.0	39.0
12	37.77775	39.0	38.0	39.0	35.0	39.0
13	37.82125	39.0	38.0	39.0	35.0	39.0
14	39.1915	41.0	39.0	41.0	36.0	41.0
15	39.0695	40.0	39.0	41.0	35.0	41.0
16	39.01225	40.0	38.0	41.0	35.0	41.0
17	39.127	40.0	39.0	41.0	36.0	41.0
18	39.07475	41.0	39.0	41.0	35.0	41.0
19	38.89275	40.0	38.0	41.0	35.0	41.0
20	39.05425	40.0	39.0	41.0	35.0	41.0
21	39.09575	40.0	39.0	41.0	35.0	41.0
22	39.087	40.0	39.0	41.0	35.0	41.0
23	38.98425	40.0	38.0	41.0	35.0	41.0
24	38.15475	40.0	38.0	41.0	33.0	41.0
25	38.2395	40.0	38.0	41.0	33.0	41.0
26	38.4055	40.0	38.0	41.0	34.0	41.0
27	38.501	40.0	38.0	41.0	34.0	41.0
28	38.5905	40.0	38.0	41.0	34.0	41.0
29	38.28625	40.0	38.0	41.0	33.0	41.0
30	38.13675	40.0	38.0	41.0	33.0	41.0
31	38.44225	40.0	38.0	41.0	34.0	41.0
32	38.634	40.0	38.0	41.0	34.0	41.0
33	37.098	40.0	37.0	41.0	31.0	41.0
34	36.4005	40.0	37.0	41.0	30.0	41.0
35	32.77425	38.0	31.0	40.0	15.0	41.0
36	29.1075	37.0	16.0	40.0	8.0	41.0
37	29.7065	35.0	23.0	39.0	6.0	41.0
38	32.14225	37.0	30.0	40.0	9.0	41.0
39	31.9255	37.0	30.0	40.0	6.0	41.0
40	32.529	37.0	32.0	40.0	2.0	41.0
41	30.67275	36.0	28.0	40.0	2.0	41.0
42	32.082	37.0	31.0	40.0	2.0	41.0
43	30.22775	37.0	26.0	40.0	2.0	41.0
44	31.37275	37.0	31.0	40.0	2.0	41.0
45	29.10525	36.0	25.0	40.0	2.0	41.0
46	27.137	34.0	15.0	39.0	2.0	41.0
47	27.32575	35.0	15.0	39.0	2.0	41.0
48	27.81275	35.0	9.0	39.0	2.0	41.0
49	26.149	35.0	2.0	39.0	2.0	41.0
50	22.50275	30.0	2.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	1.0
15	1.0
16	3.0
17	4.0
18	6.0
19	5.0
20	13.0
21	19.0
22	34.0
23	33.0
24	38.0
25	61.0
26	109.0
27	133.0
28	78.0
29	112.0
30	138.0
31	143.0
32	152.0
33	177.0
34	248.0
35	280.0
36	330.0
37	466.0
38	632.0
39	781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.994239919859755	26.771850738792885	47.28274480340596	14.951164537941397
2	24.474999999999998	27.250000000000004	20.549999999999997	27.725
3	22.25	22.35	21.85	33.550000000000004
4	29.075	28.325	15.950000000000001	26.650000000000002
5	22.8	37.675	27.150000000000002	12.375
6	34.275	17.45	34.8	13.475000000000001
7	18.425	20.325	26.900000000000002	34.35
8	28.299999999999997	30.049999999999997	14.35	27.3
9	17.974999999999998	37.25	29.825000000000003	14.95
10	22.875	22.8	27.425	26.900000000000002
11	33.175	13.175	16.8	36.85
12	17.575	20.075000000000003	27.275	35.075
13	29.125	19.025	32.7	19.15
14	32.525	27.6	23.25	16.625
15	31.95	23.225	28.95	15.875
16	18.375	19.475	22.975	39.175
17	24.0	23.575	36.725	15.7
18	24.775	18.25	30.049999999999997	26.924999999999997
19	30.775000000000002	14.774999999999999	35.199999999999996	19.25
20	41.575	15.525	28.7	14.2
21	23.400000000000002	21.925	29.099999999999998	25.575
22	28.175	12.675	46.35	12.8
23	25.775	14.399999999999999	44.0	15.825
24	49.575	14.000000000000002	26.575	9.85
25	49.325	22.925	15.2	12.55
26	28.749999999999996	44.425	14.025000000000002	12.8
27	16.525000000000002	45.95	23.625	13.900000000000002
28	13.475000000000001	25.724999999999998	46.225	14.575
29	11.375	13.825000000000001	54.900000000000006	19.900000000000002
30	11.05	7.6499999999999995	37.875	43.425000000000004
31	14.45	6.275	44.6	34.675
32	28.125	6.2	23.05	42.625
33	51.800000000000004	8.799999999999999	14.899999999999999	24.5
34	60.0	7.725	14.174999999999999	18.099999999999998
35	57.35	7.9750000000000005	22.075	12.6
36	59.824999999999996	8.875	17.0	14.299999999999999
37	39.4	12.1	21.349999999999998	27.150000000000002
38	26.775	14.05	13.3	45.875
39	18.375	25.55	9.825000000000001	46.25
40	16.425	46.475	11.525	25.575
41	26.575	45.675	11.799999999999999	15.950000000000001
42	48.85	29.175	11.05	10.925
43	46.1	32.175	11.125	10.6
44	30.5	47.575	8.275	13.65
45	18.8	45.775	10.65	24.775
46	13.05	28.475	24.474999999999998	34.0
47	10.65	16.825000000000003	32.75	39.775
48	11.25	12.9	21.625	54.225
49	14.649999999999999	23.825	13.975000000000001	47.55
50	30.332583145786447	25.28132033008252	10.527631907976994	33.85846461615404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.5
22	1.0
23	1.0
24	1.0
25	1.0
26	1.0
27	1.0
28	1.0
29	1.0
30	1.0
31	7.0
32	13.0
33	15.0
34	17.0
35	29.0
36	41.0
37	58.5
38	76.0
39	67.5
40	59.0
41	56.5
42	54.0
43	91.5
44	129.0
45	172.0
46	215.0
47	263.0
48	311.0
49	418.0
50	525.0
51	696.5
52	868.0
53	696.5
54	525.0
55	444.5
56	364.0
57	339.0
58	314.0
59	229.0
60	144.0
61	118.0
62	92.0
63	100.0
64	108.0
65	72.5
66	37.0
67	27.5
68	18.0
69	21.0
70	24.0
71	29.5
72	35.0
73	21.5
74	8.0
75	6.5
76	5.0
77	4.0
78	3.0
79	5.0
80	7.0
81	4.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.349999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83416087388282	40.699999999999996
2	7.3982125124131075	7.449999999999999
3	3.475670307845084	5.25
4	1.787487586891758	3.5999999999999996
5	1.1916583912611718	3.0
6	0.893743793445879	2.7
7	0.4468718967229395	1.575
8	0.5958291956305859	2.4
9	0.29791459781529295	1.35
>10	2.9791459781529297	26.275
>50	0.04965243296921549	2.475
>100	0.04965243296921549	3.225
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	129	3.225	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	99	2.475	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	45	1.125	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	25	0.625	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGC	21	0.525	RNA PCR Primer, Index 40 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (96% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	20	0.5	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	19	0.475	RNA PCR Primer, Index 1 (97% over 35bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	18	0.44999999999999996	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTC	17	0.42500000000000004	RNA PCR Primer, Index 40 (100% over 44bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	16	0.4	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	15	0.375	RNA PCR Primer, Index 1 (96% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	14	0.35000000000000003	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	13	0.325	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	12	0.3	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	12	0.3	No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	12	0.3	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	12	0.3	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	12	0.3	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	11	0.27499999999999997	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 29bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	9	0.22499999999999998	RNA PCR Primer, Index 2 (100% over 34bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	9	0.22499999999999998	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCG	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	8	0.2	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	7	0.17500000000000002	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	7	0.17500000000000002	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	7	0.17500000000000002	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	6	0.15	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCG	6	0.15	RNA PCR Primer, Index 1 (96% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	6	0.15	RNA PCR Primer, Index 1 (96% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	6	0.15	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	6	0.15	RNA PCR Primer, Index 1 (96% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	5	0.125	RNA PCR Primer, Index 1 (97% over 35bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	5	0.125	No Hit
GTCTGATGATGAGCCCATTGCCCTGAGCTCTTGTGATGCTGGCTTCAAAG	5	0.125	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG	5	0.125	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC	5	0.125	RNA PCR Primer, Index 1 (97% over 36bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	5	0.125	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.575	0.0	0.0	0.0
2	0.0	0.65	0.0	0.0	0.0
3	0.0	0.75	0.0	0.0	0.0
4	0.0	0.775	0.0	0.0	0.0
5	0.0	0.825	0.0	0.0	0.0
6	0.0	0.95	0.0	0.0	0.0
7	0.0	1.55	0.0	0.0	0.0
8	0.0	1.65	0.0	0.0	0.0
9	0.0	1.775	0.0	0.0	0.0
10	0.0	1.95	0.0	0.0	0.0
11	0.0	2.025	0.0	0.0	0.0
12	0.0	2.175	0.0	0.0	0.0
13	0.0	2.325	0.0	0.0	0.0
14	0.0	2.525	0.0	0.0	0.0
15	0.0	2.9	0.0	0.0	0.0
16	0.0	3.8	0.0	0.0	0.0
17	0.0	4.425	0.0	0.0	0.0
18	0.0	5.4	0.0	0.0	0.0
19	0.0	5.975	0.0	0.0	0.0
20	0.0	6.925	0.0	0.0	0.0
21	0.0	11.05	0.0	0.0	0.0
22	0.0	22.15	0.0	0.0	0.0
23	0.0	48.7	0.0	0.0	0.0
24	0.0	61.7	0.0	0.0	0.0
25	0.0	67.35	0.0	0.0	0.0
26	0.0	68.925	0.0	0.0	0.0
27	0.0	69.825	0.0	0.0	0.0
28	0.0	70.425	0.0	0.0	0.0
29	0.0	70.65	0.0	0.0	0.0
30	0.0	71.45	0.0	0.0	0.0
31	0.0	72.125	0.0	0.0	0.0
32	0.0	72.975	0.0	0.0	0.0
33	0.0	73.7	0.0	0.0	0.0
34	0.0	75.75	0.0	0.0	0.0
35	0.0	76.675	0.0	0.0	0.0
36	0.0	77.375	0.0	0.0	0.0
37	0.0	78.325	0.0	0.0	0.0
38	0.0	78.825	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAG	20	6.952051E-4	44.000004	10
CCAGTTG	20	6.952051E-4	44.000004	7
GAAGAAC	20	6.952051E-4	44.000004	13
AGAACTG	20	6.952051E-4	44.000004	15
GCCAGTT	20	6.952051E-4	44.000004	6
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGCCA	20	6.952051E-4	44.000004	3
GAACTGT	20	6.952051E-4	44.000004	16
TGCCAGT	20	6.952051E-4	44.000004	5
GCTTGGC	20	6.952051E-4	44.000004	15
TCTCTTT	20	6.952051E-4	44.000004	15
CTGCCAG	20	6.952051E-4	44.000004	4
TTGAAGA	20	6.952051E-4	44.000004	11
AAGCTGC	20	6.952051E-4	44.000004	1
AGCTGCC	20	6.952051E-4	44.000004	2
ACAGTGA	25	3.6958358E-5	44.0	3
ACCGGTC	25	3.6958358E-5	44.0	10
AACGCTG	35	1.02281774E-7	44.0	8
GAGGTAG	25	3.6958358E-5	44.0	2
GGTGAGT	25	3.6958358E-5	44.0	17
>>END_MODULE
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
Read 190240 spots for SRR5030837.sra
Written 190240 spots for SRR5030837.sra
Read 190235 spots for SRR5030837.sra
Written 190235 spots for SRR5030837.sra
SRR ids: ['SRR5030837.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fytuap71
SRR5030837.sra spots: 2282825
blocks: [[1, 190235], [190236, 380470], [380471, 570705], [570706, 760940], [760941, 951175], [951176, 1141410], [1141411, 1331645], [1331646, 1521880], [1521881, 1712115], [1712116, 1902350], [1902351, 2092585], [2092586, 2282825]]
SRR5030837 file size 394584
SRR5030837 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030837 SRR5030837_1.fastq
Input file:	SRR5030837_1.fastq
trimmed:	SRR5030837-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:24:17 2020 >> started

Wed Mar  4 06:24:18 2020 >> done (1.449s)
2282825 reads processed; of these:
    154 ( 0.01%) short reads filtered out after trimming by size control
     12 ( 0.00%) empty reads filtered out after trimming by size control
2282659 (99.99%) reads available; of these:
 262737 (11.51%) trimmed reads available after processing
2019922 (88.49%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     47	  0.00%
 19	     58	  0.00%
 20	    107	  0.00%
 21	    162	  0.01%
 22	    271	  0.01%
 23	    361	  0.02%
 24	    332	  0.01%
 25	    464	  0.02%
 26	    374	  0.02%
 27	    480	  0.02%
 28	    618	  0.03%
 29	   1083	  0.05%
 30	    747	  0.03%
 31	   1070	  0.05%
 32	   2634	  0.12%
 33	   7230	  0.32%
 34	  18971	  0.83%
 35	   4192	  0.18%
 36	   4779	  0.21%
 37	   2689	  0.12%
 38	   5549	  0.24%
 39	   4901	  0.21%
 40	   9151	  0.40%
 41	   5554	  0.24%
 42	  13084	  0.57%
 43	   6202	  0.27%
 44	  28294	  1.24%
 45	  15453	  0.68%
 46	  13557	  0.59%
 47	  15369	  0.67%
 48	  45958	  2.01%
 49	  52996	  2.32%
 50	2019922	 88.49%
2282659 reads passed initial QC


criterion=sequence-density
sequence-density=77.22
sequence-density-rank=1
fanout-score=21.62
fanout-score-rank=3
prefix-density=79.77
prefix-fanout=20.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC


criterion=fanout-score
sequence-density=1.40
sequence-density-rank=6
fanout-score=520.44
fanout-score-rank=1
prefix-density=78.86
prefix-fanout=9.2
sequence=GGAATTCTCGGTGCCAAGGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC -o SRR5030837 -
Input file:	STDIN
trimmed:	SRR5030837-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:24:32 2020 >> started

Wed Mar  4 06:24:36 2020 >> done (3.379s)
2224129 reads processed; of these:
 103069 ( 4.63%) short reads filtered out after trimming by size control
  14891 ( 0.67%) empty reads filtered out after trimming by size control
2106169 (94.70%) reads available; of these:
1795006 (85.23%) trimmed reads available after processing
 311163 (14.77%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  11416	  0.54%
 19	  20093	  0.95%
 20	  88167	  4.19%
 21	 246071	 11.68%
 22	 597063	 28.35%
 23	 292152	 13.87%
 24	 137044	  6.51%
 25	  35552	  1.69%
 26	  17840	  0.85%
 27	  13567	  0.64%
 28	  13916	  0.66%
 29	  12566	  0.60%
 30	  16109	  0.76%
 31	  23305	  1.11%
 32	  20573	  0.98%
 33	  48855	  2.32%
 34	  20371	  0.97%
 35	  16659	  0.79%
 36	  25415	  1.21%
 37	   9724	  0.46%
 38	  11616	  0.55%
 39	  10961	  0.52%
 40	  18882	  0.90%
 41	  21364	  1.01%
 42	  28318	  1.34%
 43	  18036	  0.86%
 44	  16507	  0.78%
 45	  18934	  0.90%
 46	  15216	  0.72%
 47	  11647	  0.55%
 48	   5337	  0.25%
 49	   8132	  0.39%
 50	 254761	 12.10%


criterion=sequence-density
sequence-density=6.76
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=493.97
fanout-score-rank=1
prefix-density=2.60
prefix-fanout=1.7
sequence=TTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:24:58
                             Started mapping on |	Mar 04 06:24:59
                                    Finished on |	Mar 04 06:25:23
       Mapping speed, Million of reads per hour |	324.70

                          Number of input reads |	2164699
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1148260
                        Uniquely mapped reads % |	53.04%
                          Average mapped length |	27.59
                       Number of splices: Total |	1200
            Number of splices: Annotated (sjdb) |	159
                       Number of splices: GT/AG |	1091
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	91
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.27
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	711942
             % of reads mapped to multiple loci |	32.89%
        Number of reads mapped to too many loci |	37915
             % of reads mapped to too many loci |	1.75%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.81%
                     % of reads unmapped: other |	0.51%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	304497	304497	304497
N_multimapping	711942	711942	711942
N_noFeature	60120	73537	862261
N_ambiguous	614282	369791	12132
UnstrandedReadsAssigned:473858 PositiveStrandReadsAssigned:704932 NegativeStrandReadsAssigned:273867
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030837 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030837-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,164,699 reads, 496,328 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,102 rounds

 109283 SRR5030837.ke.tsv
  52636 SRR5030837.se.tsv
 161919 total
==> SRR5030837.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030837.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030837 completed mapping pipeline successfully
