Starting /dee2/code/volunteer_pipeline.sh SRR5030881
    current disk space = 160658636800
    free memory = 56712044 
SRR5030881 SRAfilesize
4d89c6bb096cb7f0e6f41fc3f48aea0b  SRR5030881.sra
SRR5030881.sra file validated
SRR5030881 is single end
SRR5030881 is conventional basespace
SRR5030881 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030881_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5025	34.0	31.0	34.0	31.0	34.0
2	32.796	34.0	31.0	34.0	31.0	34.0
3	32.90025	34.0	31.0	34.0	31.0	34.0
4	36.27975	37.0	37.0	37.0	35.0	37.0
5	36.32575	37.0	37.0	37.0	35.0	37.0
6	36.184	37.0	37.0	37.0	35.0	37.0
7	36.09875	37.0	35.0	37.0	35.0	37.0
8	36.185	37.0	36.0	37.0	35.0	37.0
9	37.9675	39.0	38.0	39.0	35.0	39.0
10	38.049	39.0	39.0	39.0	35.0	39.0
11	37.88425	39.0	38.0	39.0	35.0	39.0
12	38.00775	39.0	38.0	39.0	35.0	39.0
13	37.88525	39.0	38.0	39.0	35.0	39.0
14	39.39075	41.0	39.0	41.0	36.0	41.0
15	39.329	41.0	39.0	41.0	36.0	41.0
16	39.3635	41.0	39.0	41.0	36.0	41.0
17	39.48575	41.0	39.0	41.0	36.0	41.0
18	39.41475	41.0	39.0	41.0	36.0	41.0
19	39.26575	41.0	39.0	41.0	36.0	41.0
20	39.15775	40.0	39.0	41.0	36.0	41.0
21	39.32075	41.0	39.0	41.0	36.0	41.0
22	39.3015	41.0	39.0	41.0	36.0	41.0
23	39.08575	41.0	39.0	41.0	35.0	41.0
24	38.95175	40.0	39.0	41.0	35.0	41.0
25	39.148	41.0	39.0	41.0	36.0	41.0
26	39.13625	41.0	39.0	41.0	36.0	41.0
27	39.17825	41.0	39.0	41.0	36.0	41.0
28	39.00175	40.0	39.0	41.0	35.0	41.0
29	38.05575	40.0	38.0	41.0	33.0	41.0
30	38.416	40.0	38.0	41.0	34.0	41.0
31	38.78275	40.0	38.0	41.0	35.0	41.0
32	39.07375	41.0	39.0	41.0	35.0	41.0
33	38.08775	40.0	38.0	41.0	33.0	41.0
34	38.129	40.0	38.0	41.0	33.0	41.0
35	37.9845	40.0	38.0	41.0	34.0	41.0
36	34.4605	38.0	31.0	40.0	23.0	41.0
37	35.66975	38.0	34.0	40.0	30.0	41.0
38	37.09	39.0	37.0	40.0	32.0	41.0
39	37.1065	39.0	37.0	40.0	32.0	41.0
40	37.11625	39.0	37.0	41.0	31.0	41.0
41	37.06875	39.0	37.0	41.0	31.0	41.0
42	37.28725	40.0	37.0	41.0	31.0	41.0
43	37.10175	40.0	37.0	41.0	31.0	41.0
44	37.08525	40.0	37.0	41.0	31.0	41.0
45	36.7125	39.0	36.0	41.0	31.0	41.0
46	35.09575	38.0	34.0	40.0	26.0	41.0
47	35.676	39.0	35.0	40.0	28.0	41.0
48	35.7875	39.0	35.0	41.0	29.0	41.0
49	34.93525	38.0	34.0	40.0	28.0	41.0
50	30.21425	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	1.0
18	1.0
19	2.0
20	0.0
21	7.0
22	8.0
23	6.0
24	9.0
25	15.0
26	21.0
27	28.0
28	32.0
29	48.0
30	44.0
31	49.0
32	79.0
33	121.0
34	151.0
35	173.0
36	295.0
37	520.0
38	1083.0
39	1303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.686686686686688	23.64864864864865	48.548548548548546	16.116116116116117
2	23.599999999999998	25.474999999999998	19.925	31.0
3	24.75	19.75	21.175	34.325
4	31.674999999999997	27.775	14.274999999999999	26.275
5	21.125	41.75	24.625	12.5
6	33.175	15.65	36.199999999999996	14.975
7	17.549999999999997	21.825	23.400000000000002	37.225
8	25.474999999999998	29.849999999999998	15.950000000000001	28.725
9	22.575	33.025	29.425	14.975
10	23.925	21.075	30.225	24.775
11	30.325000000000003	13.825000000000001	16.650000000000002	39.2
12	15.049999999999999	19.125	32.525	33.300000000000004
13	29.45	19.45	30.725	20.375
14	26.8	31.900000000000002	21.875	19.425
15	33.908477119279816	25.531382845711427	26.03150787696924	14.528632158039509
16	19.25	23.849999999999998	21.3	35.6
17	23.5	23.875	37.4	15.225
18	22.425	19.475	31.15	26.950000000000003
19	36.825	14.95	28.000000000000004	20.225
20	40.825	15.975	29.375	13.825000000000001
21	25.525	19.3	26.85	28.325
22	27.85	13.350000000000001	47.25	11.55
23	26.174999999999997	13.525	43.875	16.425
24	51.24999999999999	15.325	24.125	9.3
25	46.7	24.925	15.775	12.6
26	25.874999999999996	44.824999999999996	15.25	14.05
27	17.75	44.875	24.0	13.375
28	12.825000000000001	23.5	46.25	17.424999999999997
29	12.325	14.85	54.025	18.8
30	10.8	8.575000000000001	33.300000000000004	47.325
31	15.55	7.199999999999999	47.575	29.675
32	28.725	7.124999999999999	20.1	44.05
33	52.97500000000001	11.025	13.675	22.325
34	55.800000000000004	9.75	18.8	15.65
35	61.95	8.6	18.525	10.925
36	42.325	9.3	34.325	14.05
37	44.55	12.575	20.1	22.775000000000002
38	21.3	18.925	12.45	47.325
39	16.05	25.124999999999996	11.799999999999999	47.025
40	15.25	47.175	15.15	22.425
41	24.175	47.449999999999996	9.75	18.625
42	47.425	29.849999999999998	12.8	9.925
43	46.300000000000004	30.85	10.8	12.049999999999999
44	28.075	47.9	8.825	15.2
45	21.3	47.599999999999994	13.725000000000001	17.375
46	13.675	26.400000000000002	18.55	41.375
47	9.275	17.349999999999998	40.699999999999996	32.675
48	12.575	14.575	19.55	53.300000000000004
49	15.049999999999999	19.7	15.7	49.55
50	18.5	36.75	14.774999999999999	29.975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	1.0
10	2.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.0
24	2.0
25	1.0
26	0.0
27	1.0
28	2.0
29	2.5
30	3.0
31	11.0
32	19.0
33	22.5
34	26.0
35	38.5
36	51.0
37	70.5
38	90.0
39	77.0
40	64.0
41	55.0
42	46.0
43	85.5
44	125.0
45	170.0
46	215.0
47	310.5
48	406.0
49	512.5
50	619.0
51	713.5
52	808.0
53	629.5
54	451.0
55	463.5
56	476.0
57	366.0
58	256.0
59	157.0
60	58.0
61	52.5
62	47.0
63	92.5
64	138.0
65	88.5
66	39.0
67	26.5
68	14.0
69	12.0
70	10.0
71	12.5
72	15.0
73	10.5
74	6.0
75	4.0
76	2.0
77	3.0
78	4.0
79	3.5
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.150000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78206724782068	30.025000000000002
2	10.523038605230386	8.450000000000001
3	4.047322540473226	4.875
4	2.3661270236612704	3.8
5	1.432129514321295	2.875
6	1.2453300124533	3.0
7	0.9962640099626401	2.8000000000000003
8	0.49813200498132004	1.6
9	0.62266500622665	2.25
>10	2.9887920298879203	22.425
>50	0.311332503113325	9.225
>100	0.18679950186799502	8.674999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	120	3.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	109	2.725	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	87	2.175	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	77	1.925	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	73	1.825	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	64	1.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	50	1.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	45	1.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	39	0.975	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC	32	0.8	RNA PCR Primer, Index 42 (100% over 44bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	28	0.7000000000000001	RNA PCR Primer, Index 7 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC	19	0.475	RNA PCR Primer, Index 42 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	18	0.44999999999999996	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	18	0.44999999999999996	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	13	0.325	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	13	0.325	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 25bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	11	0.27499999999999997	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	10	0.25	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	10	0.25	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	10	0.25	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	10	0.25	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	10	0.25	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTAT	9	0.22499999999999998	RNA PCR Primer, Index 42 (100% over 48bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	9	0.22499999999999998	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	9	0.22499999999999998	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	9	0.22499999999999998	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	8	0.2	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	7	0.17500000000000002	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	7	0.17500000000000002	RNA PCR Primer, Index 3 (100% over 34bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	7	0.17500000000000002	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC	6	0.15	RNA PCR Primer, Index 42 (100% over 38bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	6	0.15	No Hit
GTCCATGATGAGCAACACTCACCATCTTTCGTTTGAGTCTCACGACTGTG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GACCCTGTTGATCTCAAATACTAATTGCCAGTTTTGTCTGATGTGGAATT	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA	5	0.125	RNA PCR Primer, Index 3 (97% over 36bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.525	0.0	0.0	0.0
2	0.0	0.575	0.0	0.0	0.0
3	0.0	0.8	0.0	0.0	0.0
4	0.0	0.825	0.0	0.0	0.0
5	0.0	0.875	0.0	0.0	0.0
6	0.0	0.95	0.0	0.0	0.0
7	0.0	1.925	0.0	0.0	0.0
8	0.0	2.05	0.0	0.0	0.0
9	0.0	2.2	0.0	0.0	0.0
10	0.0	2.325	0.0	0.0	0.0
11	0.0	2.625	0.0	0.0	0.0
12	0.0	2.975	0.0	0.0	0.0
13	0.0	3.525	0.0	0.0	0.0
14	0.0	3.725	0.0	0.0	0.0
15	0.0	4.05	0.0	0.0	0.0
16	0.0	5.4	0.0	0.0	0.0
17	0.0	6.1	0.0	0.0	0.0
18	0.0	7.05	0.0	0.0	0.0
19	0.0	7.7	0.0	0.0	0.0
20	0.0	8.675	0.0	0.0	0.0
21	0.0	14.1	0.0	0.0	0.0
22	0.0	24.275	0.0	0.0	0.0
23	0.0	52.775	0.0	0.0	0.0
24	0.0	62.925	0.0	0.0	0.0
25	0.0	68.75	0.0	0.0	0.0
26	0.0	70.15	0.0	0.0	0.0
27	0.0	70.725	0.0	0.0	0.0
28	0.0	71.075	0.0	0.0	0.0
29	0.0	71.55	0.0	0.0	0.0
30	0.0	71.9	0.0	0.0	0.0
31	0.0	72.85	0.0	0.0	0.0
32	0.0	73.6	0.0	0.0	0.0
33	0.0	74.2	0.0	0.0	0.0
34	0.0	77.875	0.0	0.0	0.0
35	0.0	78.95	0.0	0.0	0.0
36	0.0	79.675	0.0	0.0	0.0
37	0.0	80.775	0.0	0.0	0.0
38	0.0	81.175	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGCT	45	2.728484E-10	44.000004	11
CTCTTTT	20	6.952051E-4	44.000004	16
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
TCGGATC	45	2.728484E-10	44.000004	1
CGGATCC	45	2.728484E-10	44.000004	2
CTTGGTG	20	6.952051E-4	44.000004	16
ACATTCA	20	6.952051E-4	44.000004	2
GGGGGCC	20	6.952051E-4	44.000004	33
GCTGTCG	20	6.952051E-4	44.000004	11
GATCCGT	45	2.728484E-10	44.000004	4
GGCCAAG	20	6.952051E-4	44.000004	36
GCTTGGT	20	6.952051E-4	44.000004	15
TTGGTGG	20	6.952051E-4	44.000004	17
TCCGTCT	45	2.728484E-10	44.000004	6
GGGGCCA	20	6.952051E-4	44.000004	34
ATCCGTC	45	2.728484E-10	44.000004	5
CTGTCGG	20	6.952051E-4	44.000004	12
TGAGCTT	45	2.728484E-10	44.000004	12
>>END_MODULE
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197690 spots for SRR5030881.sra
Written 197690 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
Read 197689 spots for SRR5030881.sra
Written 197689 spots for SRR5030881.sra
SRR ids: ['SRR5030881.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qlatn78t
SRR5030881.sra spots: 2372269
blocks: [[1, 197689], [197690, 395378], [395379, 593067], [593068, 790756], [790757, 988445], [988446, 1186134], [1186135, 1383823], [1383824, 1581512], [1581513, 1779201], [1779202, 1976890], [1976891, 2174579], [2174580, 2372269]]
SRR5030881 file size 410089
SRR5030881 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030881 SRR5030881_1.fastq
Input file:	SRR5030881_1.fastq
trimmed:	SRR5030881-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:34:57 2020 >> started

Wed Mar  4 06:34:59 2020 >> done (1.542s)
2372269 reads processed; of these:
     98 ( 0.00%) short reads filtered out after trimming by size control
      2 ( 0.00%) empty reads filtered out after trimming by size control
2372169 (100.00%) reads available; of these:
 141749 ( 5.98%) trimmed reads available after processing
2230420 (94.02%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     39	  0.00%
 19	     43	  0.00%
 20	    102	  0.00%
 21	    125	  0.01%
 22	    232	  0.01%
 23	    228	  0.01%
 24	    298	  0.01%
 25	    334	  0.01%
 26	    272	  0.01%
 27	    407	  0.02%
 28	    568	  0.02%
 29	    670	  0.03%
 30	    525	  0.02%
 31	    751	  0.03%
 32	    716	  0.03%
 33	    916	  0.04%
 34	   2107	  0.09%
 35	   2095	  0.09%
 36	   1569	  0.07%
 37	   1746	  0.07%
 38	   1971	  0.08%
 39	   1963	  0.08%
 40	   2344	  0.10%
 41	   2635	  0.11%
 42	   3277	  0.14%
 43	   4482	  0.19%
 44	   8231	  0.35%
 45	  10447	  0.44%
 46	  10038	  0.42%
 47	  14648	  0.62%
 48	  26628	  1.12%
 49	  41342	  1.74%
 50	2230420	 94.02%
2372169 reads passed initial QC


criterion=sequence-density
sequence-density=80.94
sequence-density-rank=1
fanout-score=18.81
fanout-score-rank=1
prefix-density=82.45
prefix-fanout=18.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC


criterion=fanout-score
sequence-density=80.94
sequence-density-rank=1
fanout-score=18.81
fanout-score-rank=1
prefix-density=82.45
prefix-fanout=18.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC -o SRR5030881 -
Input file:	STDIN
trimmed:	SRR5030881-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:35:11 2020 >> started

Wed Mar  4 06:35:15 2020 >> done (3.626s)
2313597 reads processed; of these:
 149748 ( 6.47%) short reads filtered out after trimming by size control
  16420 ( 0.71%) empty reads filtered out after trimming by size control
2147429 (92.82%) reads available; of these:
1896048 (88.29%) trimmed reads available after processing
 251381 (11.71%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15644	  0.73%
 19	  21125	  0.98%
 20	 121455	  5.66%
 21	 250660	 11.67%
 22	 647525	 30.15%
 23	 259949	 12.11%
 24	 121117	  5.64%
 25	  31554	  1.47%
 26	  15974	  0.74%
 27	  13179	  0.61%
 28	  13917	  0.65%
 29	  10044	  0.47%
 30	  15315	  0.71%
 31	  19906	  0.93%
 32	  19932	  0.93%
 33	  87747	  4.09%
 34	  25906	  1.21%
 35	  21358	  0.99%
 36	  24322	  1.13%
 37	  10952	  0.51%
 38	  11445	  0.53%
 39	  12102	  0.56%
 40	  16343	  0.76%
 41	  22091	  1.03%
 42	  25418	  1.18%
 43	  17741	  0.83%
 44	  15453	  0.72%
 45	  18149	  0.85%
 46	  13228	  0.62%
 47	  11966	  0.56%
 48	   2709	  0.13%
 49	   9167	  0.43%
 50	 224036	 10.43%


criterion=sequence-density
sequence-density=4.51
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.61
sequence-density-rank=19
fanout-score=13.64
fanout-score-rank=1
prefix-density=8.28
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:35:36
                             Started mapping on |	Mar 04 06:35:36
                                    Finished on |	Mar 04 06:36:01
       Mapping speed, Million of reads per hour |	317.66

                          Number of input reads |	2206001
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1250672
                        Uniquely mapped reads % |	56.69%
                          Average mapped length |	27.49
                       Number of splices: Total |	853
            Number of splices: Annotated (sjdb) |	112
                       Number of splices: GT/AG |	797
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	43
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	675382
             % of reads mapped to multiple loci |	30.62%
        Number of reads mapped to too many loci |	54831
             % of reads mapped to too many loci |	2.49%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.70%
                     % of reads unmapped: other |	0.51%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	279947	279947	279947
N_multimapping	675382	675382	675382
N_noFeature	50346	63291	881175
N_ambiguous	713558	396759	11674
UnstrandedReadsAssigned:486768 PositiveStrandReadsAssigned:790622 NegativeStrandReadsAssigned:357823
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030881 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030881-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,206,001 reads, 594,783 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 847 rounds

 109283 SRR5030881.ke.tsv
  52636 SRR5030881.se.tsv
 161919 total
==> SRR5030881.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030881.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030881 completed mapping pipeline successfully
