Starting /dee2/code/volunteer_pipeline.sh SRR5030996
    current disk space = 160569540608
    free memory = 73949356 
SRR5030996 SRAfilesize
ccb43963f13255885babe5b5aa5960e6  SRR5030996.sra
SRR5030996.sra file validated
SRR5030996 is single end
SRR5030996 is conventional basespace
SRR5030996 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26675	33.0	31.0	34.0	31.0	34.0
2	32.4925	34.0	31.0	34.0	31.0	34.0
3	32.55425	34.0	31.0	34.0	31.0	34.0
4	36.15475	37.0	35.0	37.0	35.0	37.0
5	36.06325	37.0	35.0	37.0	35.0	37.0
6	35.96525	37.0	35.0	37.0	35.0	37.0
7	35.874	37.0	35.0	37.0	35.0	37.0
8	35.9655	37.0	35.0	37.0	35.0	37.0
9	37.566	39.0	37.0	39.0	35.0	39.0
10	37.449	39.0	37.0	39.0	34.0	39.0
11	37.054	39.0	37.0	39.0	33.0	39.0
12	37.454	39.0	37.0	39.0	34.0	39.0
13	37.505	39.0	37.0	39.0	34.0	39.0
14	38.84375	40.0	38.0	41.0	35.0	41.0
15	38.8685	40.0	38.0	41.0	35.0	41.0
16	38.8875	40.0	38.0	41.0	35.0	41.0
17	38.92775	40.0	38.0	41.0	35.0	41.0
18	38.6815	40.0	38.0	41.0	34.0	41.0
19	38.5475	40.0	38.0	41.0	34.0	41.0
20	38.54875	40.0	38.0	41.0	34.0	41.0
21	38.62675	40.0	38.0	41.0	34.0	41.0
22	38.67325	40.0	38.0	41.0	34.0	41.0
23	38.2825	40.0	38.0	41.0	33.0	41.0
24	38.62275	40.0	38.0	41.0	34.0	41.0
25	38.71525	40.0	38.0	41.0	35.0	41.0
26	38.217	40.0	38.0	41.0	33.0	41.0
27	38.435	40.0	38.0	41.0	34.0	41.0
28	38.33125	40.0	38.0	41.0	34.0	41.0
29	38.0865	40.0	38.0	41.0	33.0	41.0
30	37.95625	40.0	38.0	41.0	33.0	41.0
31	38.07375	40.0	38.0	41.0	33.0	41.0
32	38.25125	40.0	38.0	41.0	33.0	41.0
33	37.87	40.0	38.0	41.0	33.0	41.0
34	37.983	40.0	38.0	41.0	33.0	41.0
35	37.8145	40.0	37.0	41.0	33.0	41.0
36	37.6045	40.0	37.0	41.0	32.0	41.0
37	37.03125	39.0	36.0	41.0	31.0	41.0
38	36.92875	39.0	36.0	40.0	31.0	41.0
39	37.46325	39.0	37.0	41.0	32.0	41.0
40	37.57775	40.0	37.0	41.0	33.0	41.0
41	37.71775	40.0	37.0	41.0	33.0	41.0
42	37.71025	40.0	37.0	41.0	33.0	41.0
43	37.6685	40.0	37.0	41.0	33.0	41.0
44	37.754	40.0	37.0	41.0	33.0	41.0
45	37.66975	40.0	37.0	41.0	33.0	41.0
46	37.30275	40.0	37.0	41.0	32.0	41.0
47	36.701	40.0	35.0	41.0	30.0	41.0
48	36.436	39.0	35.0	41.0	30.0	41.0
49	36.489	39.0	35.0	41.0	30.0	41.0
50	35.81075	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	1.0
14	0.0
15	2.0
16	0.0
17	0.0
18	1.0
19	1.0
20	3.0
21	1.0
22	5.0
23	9.0
24	6.0
25	5.0
26	24.0
27	25.0
28	30.0
29	32.0
30	56.0
31	61.0
32	91.0
33	140.0
34	164.0
35	219.0
36	294.0
37	463.0
38	784.0
39	1580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.85	26.275	44.7	16.175
2	21.344369199899674	29.29520943064961	19.889641334336595	29.470780035114117
3	24.525	17.8	24.325	33.35
4	33.900000000000006	28.999999999999996	16.675	20.424999999999997
5	22.95	35.225	27.275	14.549999999999999
6	28.525	15.125	39.675	16.675
7	20.125	19.925	20.05	39.900000000000006
8	21.15	30.45	18.099999999999998	30.3
9	24.8	32.125	25.85	17.224999999999998
10	26.025	17.75	29.15	27.075
11	33.45	12.525	17.675	36.35
12	16.525000000000002	19.175	32.475	31.825
13	28.1	17.599999999999998	32.975	21.325
14	30.575000000000003	32.4	19.05	17.974999999999998
15	38.074999999999996	21.675	25.424999999999997	14.825
16	17.849999999999998	18.7	25.674999999999997	37.775
17	29.825000000000003	20.325	34.775	15.075
18	27.975	15.950000000000001	32.425	23.65
19	35.375	14.149999999999999	33.275	17.2
20	45.75	17.349999999999998	23.225	13.675
21	23.45	23.375	23.325000000000003	29.849999999999998
22	30.15	15.35	42.75	11.75
23	24.725	14.099999999999998	44.4	16.775000000000002
24	48.5	15.0	26.775	9.725
25	49.275000000000006	22.075	16.35	12.3
26	28.425	43.35	14.875	13.350000000000001
27	15.9	45.25	24.0	14.85
28	13.725000000000001	23.275000000000002	46.35	16.650000000000002
29	14.249999999999998	12.950000000000001	54.125	18.675
30	12.4	8.15	33.75	45.7
31	15.4	6.0249999999999995	44.725	33.85
32	27.075	6.225	24.2	42.5
33	51.2	8.85	14.725	25.224999999999998
34	57.99999999999999	7.95	16.875	17.175
35	61.324999999999996	8.5	18.0	12.174999999999999
36	40.325	9.725	35.85	14.099999999999998
37	44.65	11.05	22.175	22.125
38	26.974999999999998	16.825000000000003	12.7	43.5
39	16.900000000000002	22.75	11.5	48.85
40	16.775000000000002	45.7	12.625	24.9
41	23.05	47.75	11.225	17.974999999999998
42	46.425	27.175	13.950000000000001	12.45
43	51.125	25.55	10.6	12.725
44	31.85	44.425	8.7	15.024999999999999
45	19.275000000000002	48.275	13.950000000000001	18.5
46	16.625	25.324999999999996	16.525000000000002	41.525
47	12.125	15.049999999999999	38.125	34.699999999999996
48	12.15	12.8	22.35	52.7
49	13.900000000000002	17.7	15.075	53.325
50	17.375	35.55	14.124999999999998	32.95
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.5
24	2.0
25	1.5
26	1.0
27	1.0
28	1.0
29	2.5
30	4.0
31	10.5
32	17.0
33	15.0
34	13.0
35	21.5
36	30.0
37	35.0
38	40.0
39	41.5
40	43.0
41	45.5
42	48.0
43	80.5
44	113.0
45	139.0
46	165.0
47	277.0
48	389.0
49	479.5
50	570.0
51	698.5
52	827.0
53	633.0
54	439.0
55	484.0
56	529.0
57	417.5
58	306.0
59	208.0
60	110.0
61	81.0
62	52.0
63	79.5
64	107.0
65	72.0
66	37.0
67	27.5
68	18.0
69	26.5
70	35.0
71	64.0
72	93.0
73	50.0
74	7.0
75	4.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.325
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.900000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31328320802005	30.049999999999997
2	10.526315789473683	8.4
3	3.8847117794486214	4.65
4	2.1303258145363406	3.4000000000000004
5	1.8796992481203008	3.75
6	1.3784461152882206	3.3000000000000003
7	0.7518796992481203	2.1
8	0.5639097744360901	1.7999999999999998
9	0.2506265664160401	0.8999999999999999
>10	2.756892230576441	20.1
>50	0.37593984962406013	11.225
>100	0.18796992481203006	10.325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	147	3.675	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	144	3.5999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	122	3.05	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	99	2.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	98	2.45	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	86	2.15	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	58	1.4500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	53	1.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	45	1.125	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC	27	0.675	RNA PCR Primer, Index 5 (97% over 35bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	23	0.575	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	18	0.44999999999999996	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	16	0.4	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	16	0.4	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	15	0.375	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	15	0.375	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC	12	0.3	RNA PCR Primer, Index 16 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	12	0.3	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	11	0.27499999999999997	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	10	0.25	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	9	0.22499999999999998	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	8	0.2	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 16 (100% over 50bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	6	0.15	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGAATTGTGGCTGGACATCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGCATTCTCGGGTGCC	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTC	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCTGGAATTCTCGGGTG	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGATCAGAAGGTGACTGTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGTCCCCTTCAACCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.225	0.0	0.0	0.0
2	0.0	0.225	0.0	0.0	0.0
3	0.0	0.325	0.0	0.0	0.0
4	0.0	0.35	0.0	0.0	0.0
5	0.0	0.4	0.0	0.0	0.0
6	0.0	0.45	0.0	0.0	0.0
7	0.0	0.85	0.0	0.0	0.0
8	0.0	1.05	0.0	0.0	0.0
9	0.0	1.075	0.0	0.0	0.0
10	0.0	1.275	0.0	0.0	0.0
11	0.0	1.4	0.0	0.0	0.0
12	0.0	1.525	0.0	0.0	0.0
13	0.0	1.675	0.0	0.0	0.0
14	0.0	1.875	0.0	0.0	0.0
15	0.0	2.175	0.0	0.0	0.0
16	0.0	3.475	0.0	0.0	0.0
17	0.0	4.45	0.0	0.0	0.0
18	0.0	5.825	0.0	0.0	0.0
19	0.0	6.5	0.0	0.0	0.0
20	0.0	7.575	0.0	0.0	0.0
21	0.0	12.375	0.0	0.0	0.0
22	0.0	21.65	0.0	0.0	0.0
23	0.0	49.225	0.0	0.0	0.0
24	0.0	61.825	0.0	0.0	0.0
25	0.0	66.875	0.0	0.0	0.0
26	0.0	68.7	0.0	0.0	0.0
27	0.0	69.65	0.0	0.0	0.0
28	0.0	70.425	0.0	0.0	0.0
29	0.0	71.0	0.0	0.0	0.0
30	0.0	71.8	0.0	0.0	0.0
31	0.0	72.6	0.0	0.0	0.0
32	0.0	74.075	0.0	0.0	0.0
33	0.0	75.25	0.0	0.0	0.0
34	0.0	78.3	0.0	0.0	0.0
35	0.0	79.9	0.0	0.0	0.0
36	0.0	81.175	0.0	0.0	0.0
37	0.0	82.9	0.0	0.0	0.0
38	0.0	83.35	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATTCA	25	3.4343255E-5	44.531647	2
AACATTC	25	3.4343255E-5	44.531647	1
TGTAAAC	25	3.4343255E-5	44.531647	1
GTAAACA	25	3.4343255E-5	44.531647	2
GAGGTAG	20	6.54202E-4	44.531643	2
TCAAGTA	20	6.54202E-4	44.531643	2
CATTGGT	20	6.54202E-4	44.531643	2
TTCAAGT	20	6.54202E-4	44.531643	1
TCGGATC	40	4.7512003E-9	44.531643	1
CGGATCC	40	4.7512003E-9	44.531643	2
AACGCTG	25	3.7082697E-5	43.975002	8
ACGCTGT	25	3.7082697E-5	43.975002	9
TAAACAT	25	3.7082697E-5	43.975002	3
AAACATC	25	3.7082697E-5	43.975002	4
TTCAACG	25	3.7082697E-5	43.975002	5
TCGGTGA	25	3.7082697E-5	43.975002	15
CATTCAA	25	3.7082697E-5	43.975002	3
TCAACGC	25	3.7082697E-5	43.975002	6
CTTGGCT	30	1.9592044E-6	43.975002	16
GCTGTCG	25	3.7082697E-5	43.975002	11
>>END_MODULE
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229551 spots for SRR5030996.sra
Written 229551 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
Read 229550 spots for SRR5030996.sra
Written 229550 spots for SRR5030996.sra
SRR ids: ['SRR5030996.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_h5ik81j2
SRR5030996.sra spots: 2754601
blocks: [[1, 229550], [229551, 459100], [459101, 688650], [688651, 918200], [918201, 1147750], [1147751, 1377300], [1377301, 1606850], [1606851, 1836400], [1836401, 2065950], [2065951, 2295500], [2295501, 2525050], [2525051, 2754601]]
SRR5030996 file size 476364
SRR5030996 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030996 SRR5030996_1.fastq
Input file:	SRR5030996_1.fastq
trimmed:	SRR5030996-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:02:37 2020 >> started

Wed Mar  4 07:02:40 2020 >> done (2.935s)
2754601 reads processed; of these:
    267 ( 0.01%) short reads filtered out after trimming by size control
      7 ( 0.00%) empty reads filtered out after trimming by size control
2754327 (99.99%) reads available; of these:
 179457 ( 6.52%) trimmed reads available after processing
2574870 (93.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     99	  0.00%
 19	     96	  0.00%
 20	    167	  0.01%
 21	    202	  0.01%
 22	    365	  0.01%
 23	    436	  0.02%
 24	    440	  0.02%
 25	    627	  0.02%
 26	    507	  0.02%
 27	    652	  0.02%
 28	    760	  0.03%
 29	   1171	  0.04%
 30	    982	  0.04%
 31	    890	  0.03%
 32	   1041	  0.04%
 33	   1119	  0.04%
 34	   1341	  0.05%
 35	   2472	  0.09%
 36	   2258	  0.08%
 37	   2692	  0.10%
 38	   2723	  0.10%
 39	   3124	  0.11%
 40	   3325	  0.12%
 41	   3555	  0.13%
 42	   4771	  0.17%
 43	   6633	  0.24%
 44	   9137	  0.33%
 45	  12336	  0.45%
 46	  18451	  0.67%
 47	  21892	  0.79%
 48	  33038	  1.20%
 49	  42155	  1.53%
 50	2574870	 93.48%
2754327 reads passed initial QC


criterion=sequence-density
sequence-density=82.65
sequence-density-rank=1
fanout-score=22.13
fanout-score-rank=1
prefix-density=85.54
prefix-fanout=21.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC


criterion=fanout-score
sequence-density=82.65
sequence-density-rank=1
fanout-score=22.13
fanout-score-rank=1
prefix-density=85.54
prefix-fanout=21.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC -o SRR5030996 -
Input file:	STDIN
trimmed:	SRR5030996-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:02:58 2020 >> started

Wed Mar  4 07:03:02 2020 >> done (4.110s)
2687958 reads processed; of these:
 160520 ( 5.97%) short reads filtered out after trimming by size control
   7652 ( 0.28%) empty reads filtered out after trimming by size control
2519786 (93.74%) reads available; of these:
2355320 (93.47%) trimmed reads available after processing
 164466 ( 6.53%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  14412	  0.57%
 19	  27239	  1.08%
 20	 135715	  5.39%
 21	 265789	 10.55%
 22	 715563	 28.40%
 23	 334181	 13.26%
 24	 151726	  6.02%
 25	  44200	  1.75%
 26	  23937	  0.95%
 27	  19453	  0.77%
 28	  21733	  0.86%
 29	  18079	  0.72%
 30	  26955	  1.07%
 31	  35825	  1.42%
 32	  37934	  1.51%
 33	 103938	  4.12%
 34	  43285	  1.72%
 35	  35785	  1.42%
 36	  42553	  1.69%
 37	  18427	  0.73%
 38	  21859	  0.87%
 39	  18658	  0.74%
 40	  29378	  1.17%
 41	  34496	  1.37%
 42	  38596	  1.53%
 43	  28258	  1.12%
 44	  20360	  0.81%
 45	  29005	  1.15%
 46	  23041	  0.91%
 47	  17222	  0.68%
 48	   1867	  0.07%
 49	   2848	  0.11%
 50	 137469	  5.46%


criterion=sequence-density
sequence-density=2.49
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AAGCTGCCAGTTGAAGAACTGTA


criterion=fanout-score
sequence-density=0.46
sequence-density-rank=31
fanout-score=17.93
fanout-score-rank=1
prefix-density=8.26
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 07:03:33
                             Started mapping on |	Mar 04 07:03:34
                                    Finished on |	Mar 04 07:03:58
       Mapping speed, Million of reads per hour |	387.92

                          Number of input reads |	2586155
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1350021
                        Uniquely mapped reads % |	52.20%
                          Average mapped length |	25.25
                       Number of splices: Total |	1831
            Number of splices: Annotated (sjdb) |	601
                       Number of splices: GT/AG |	1715
                       Number of splices: GC/AG |	17
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	96
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.86
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	820590
             % of reads mapped to multiple loci |	31.73%
        Number of reads mapped to too many loci |	74429
             % of reads mapped to too many loci |	2.88%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.16%
                     % of reads unmapped: other |	1.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	415544	415544	415544
N_multimapping	820590	820590	820590
N_noFeature	69950	92185	898016
N_ambiguous	726497	331906	14671
UnstrandedReadsAssigned:553574 PositiveStrandReadsAssigned:925930 NegativeStrandReadsAssigned:437334
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030996 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030996-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,586,155 reads, 631,062 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,163 rounds

 109283 SRR5030996.ke.tsv
  52636 SRR5030996.se.tsv
 161919 total
==> SRR5030996.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030996.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030996 completed mapping pipeline successfully
