Starting /dee2/code/volunteer_pipeline.sh SRR5030916
    current disk space = 160629506048
    free memory = 73939200 
SRR5030916 SRAfilesize
4f3a9e058e7ae88814b4565795ca336a  SRR5030916.sra
SRR5030916.sra file validated
SRR5030916 is single end
SRR5030916 is conventional basespace
SRR5030916 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.366	33.0	31.0	34.0	31.0	34.0
2	32.67375	34.0	31.0	34.0	31.0	34.0
3	32.78425	34.0	31.0	34.0	31.0	34.0
4	36.21075	37.0	37.0	37.0	35.0	37.0
5	36.31025	37.0	37.0	37.0	35.0	37.0
6	36.11125	37.0	35.0	37.0	35.0	37.0
7	36.1275	37.0	35.0	37.0	35.0	37.0
8	36.22775	37.0	36.0	37.0	35.0	37.0
9	37.9925	39.0	38.0	39.0	35.0	39.0
10	38.0405	39.0	38.0	39.0	35.0	39.0
11	37.86625	39.0	38.0	39.0	35.0	39.0
12	37.9055	39.0	38.0	39.0	35.0	39.0
13	37.7625	39.0	38.0	39.0	35.0	39.0
14	39.24725	41.0	39.0	41.0	36.0	41.0
15	39.28575	41.0	39.0	41.0	36.0	41.0
16	39.256	40.0	39.0	41.0	36.0	41.0
17	39.28375	41.0	39.0	41.0	36.0	41.0
18	39.1505	41.0	39.0	41.0	36.0	41.0
19	38.80875	40.0	38.0	41.0	34.0	41.0
20	38.9455	40.0	38.0	41.0	35.0	41.0
21	38.96825	40.0	38.0	41.0	35.0	41.0
22	39.12925	40.0	39.0	41.0	36.0	41.0
23	38.909	40.0	39.0	41.0	35.0	41.0
24	38.583	40.0	38.0	41.0	34.0	41.0
25	38.785	40.0	38.0	41.0	35.0	41.0
26	38.507	40.0	38.0	41.0	34.0	41.0
27	38.6795	40.0	38.0	41.0	35.0	41.0
28	38.67425	40.0	38.0	41.0	34.0	41.0
29	38.203	40.0	38.0	41.0	34.0	41.0
30	38.509	40.0	38.0	41.0	34.0	41.0
31	38.62325	40.0	38.0	41.0	34.0	41.0
32	38.81425	40.0	38.0	41.0	35.0	41.0
33	38.15225	40.0	38.0	41.0	33.0	41.0
34	38.26875	40.0	38.0	41.0	33.0	41.0
35	38.056	40.0	38.0	41.0	33.0	41.0
36	34.16025	38.0	31.0	40.0	23.0	41.0
37	35.8105	38.0	35.0	40.0	30.0	41.0
38	37.11125	39.0	37.0	40.0	32.0	41.0
39	37.19925	39.0	37.0	40.0	31.0	41.0
40	37.0695	39.0	37.0	41.0	31.0	41.0
41	37.2905	40.0	37.0	41.0	32.0	41.0
42	37.548	40.0	38.0	41.0	33.0	41.0
43	37.317	40.0	37.0	41.0	32.0	41.0
44	37.2005	40.0	37.0	41.0	32.0	41.0
45	37.08375	40.0	37.0	41.0	32.0	41.0
46	35.26975	38.0	35.0	40.0	26.0	41.0
47	35.8365	39.0	35.0	40.0	28.0	41.0
48	36.1945	39.0	36.0	41.0	30.0	41.0
49	35.22325	39.0	35.0	40.0	28.0	41.0
50	30.0285	34.0	27.0	39.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	2.0
17	2.0
18	1.0
19	1.0
20	5.0
21	10.0
22	11.0
23	15.0
24	9.0
25	19.0
26	10.0
27	28.0
28	25.0
29	29.0
30	47.0
31	56.0
32	86.0
33	105.0
34	176.0
35	220.0
36	289.0
37	484.0
38	1030.0
39	1337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.749059914765606	20.832288794184006	56.6307345199298	13.787916771120582
2	23.0	26.900000000000002	23.0	27.1
3	18.25	20.375	19.675	41.699999999999996
4	25.124999999999996	36.325	13.575000000000001	24.975
5	21.4	40.475	25.75	12.375
6	37.475	15.6	36.3	10.625
7	14.249999999999998	23.325000000000003	27.375	35.05
8	23.724999999999998	42.75	12.7	20.825
9	20.625	38.074999999999996	30.025000000000002	11.275
10	21.2	20.200000000000003	28.1	30.5
11	28.499999999999996	14.725	14.149999999999999	42.625
12	14.95	16.425	25.074999999999996	43.55
13	20.349999999999998	23.799999999999997	34.75	21.099999999999998
14	37.724999999999994	24.625	24.725	12.925
15	37.8	23.849999999999998	26.200000000000003	12.15
16	11.475	29.275000000000002	20.150000000000002	39.1
17	23.3	21.125	41.225	14.35
18	27.650000000000002	19.125	30.425	22.8
19	39.825	14.6	29.5	16.075
20	50.0	18.25	21.325	10.424999999999999
21	20.4	24.7	23.9	31.0
22	31.5	11.075	50.375	7.049999999999999
23	26.200000000000003	12.5	48.199999999999996	13.100000000000001
24	52.225	14.099999999999998	26.424999999999997	7.249999999999999
25	53.900000000000006	23.775	13.425	8.9
26	28.549999999999997	49.75	12.075	9.625
27	13.5	52.849999999999994	24.575	9.075
28	9.4	25.924999999999997	51.9	12.775
29	7.85	11.275	61.1	19.775000000000002
30	9.1	5.949999999999999	37.775	47.175
31	12.950000000000001	4.925	47.025	35.099999999999994
32	26.3	5.775	23.0	44.925
33	57.15	7.225	12.35	23.275000000000002
34	68.2	6.25	12.825000000000001	12.725
35	66.75	6.4750000000000005	17.974999999999998	8.799999999999999
36	44.074999999999996	7.449999999999999	36.275	12.2
37	45.225	9.775	21.75	23.25
38	23.05	13.625000000000002	11.825	51.5
39	13.55	23.7	7.8	54.949999999999996
40	13.600000000000001	51.300000000000004	8.825	26.275
41	24.15	54.15	7.6	14.099999999999998
42	54.1	30.7	7.85	7.35
43	55.85	29.275000000000002	7.375	7.5
44	29.15	51.775000000000006	6.4	12.675
45	14.224999999999998	55.35	11.525	18.9
46	8.7	27.675	19.15	44.474999999999994
47	7.625	13.475000000000001	40.8	38.1
48	8.425	11.425	21.349999999999998	58.8
49	12.125	19.3	11.3	57.275
50	19.675	38.074999999999996	11.075	31.175000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	2.5
30	3.0
31	5.0
32	7.0
33	16.0
34	25.0
35	32.5
36	40.0
37	49.0
38	58.0
39	46.5
40	35.0
41	45.5
42	56.0
43	94.0
44	132.0
45	189.5
46	247.0
47	411.0
48	575.0
49	581.0
50	587.0
51	737.5
52	888.0
53	625.5
54	363.0
55	368.5
56	374.0
57	361.0
58	348.0
59	215.0
60	82.0
61	61.5
62	41.0
63	63.0
64	85.0
65	53.0
66	21.0
67	15.0
68	9.0
69	7.0
70	5.0
71	7.5
72	10.0
73	5.5
74	1.0
75	0.5
76	0.0
77	1.5
78	3.0
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27499999999999997
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01647989452867	28.449999999999996
2	11.140408701384311	8.450000000000001
3	3.2300593276203036	3.675
4	1.7139090309822018	2.6
5	1.977587343441002	3.75
6	0.988793671720501	2.25
7	0.6591957811470006	1.7500000000000002
8	0.5932762030323006	1.7999999999999998
9	0.5932762030323006	2.025
>10	3.691496374423204	27.3
>50	0.13183915622940012	3.175
>100	0.26367831245880025	14.774999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	239	5.975	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	130	3.25	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	112	2.8000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	110	2.75	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	45	1.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	38	0.95	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	32	0.8	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	30	0.75	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	28	0.7000000000000001	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	22	0.5499999999999999	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	20	0.5	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC	19	0.475	RNA PCR Primer, Index 5 (97% over 35bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC	14	0.35000000000000003	RNA PCR Primer, Index 16 (100% over 44bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	12	0.3	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	12	0.3	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	9	0.22499999999999998	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	9	0.22499999999999998	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACAGAAATATTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	8	0.2	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 16 (100% over 50bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	6	0.15	RNA PCR Primer, Index 1 (96% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	6	0.15	RNA PCR Primer, Index 1 (100% over 32bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	6	0.15	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	5	0.125	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTAAGGCACGCGGTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	5	0.125	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.175	0.0	0.0	0.0
2	0.0	0.2	0.0	0.0	0.0
3	0.0	0.3	0.0	0.0	0.0
4	0.0	0.3	0.0	0.0	0.0
5	0.0	0.325	0.0	0.0	0.0
6	0.0	0.45	0.0	0.0	0.0
7	0.0	0.875	0.0	0.0	0.0
8	0.0	1.05	0.0	0.0	0.0
9	0.0	1.125	0.0	0.0	0.0
10	0.0	1.225	0.0	0.0	0.0
11	0.0	1.425	0.0	0.0	0.0
12	0.0	1.525	0.0	0.0	0.0
13	0.0	1.75	0.0	0.0	0.0
14	0.0	1.975	0.0	0.0	0.0
15	0.0	2.175	0.0	0.0	0.0
16	0.0	3.125	0.0	0.0	0.0
17	0.0	3.4	0.0	0.0	0.0
18	0.0	4.225	0.0	0.0	0.0
19	0.0	4.8	0.0	0.0	0.0
20	0.0	5.925	0.0	0.0	0.0
21	0.0	11.325	0.0	0.0	0.0
22	0.0	23.55	0.0	0.0	0.0
23	0.0	56.375	0.0	0.0	0.0
24	0.0	71.55	0.0	0.0	0.0
25	0.0	76.7	0.0	0.0	0.0
26	0.0	77.5	0.0	0.0	0.0
27	0.0	77.875	0.0	0.0	0.0
28	0.0	78.275	0.0	0.0	0.0
29	0.0	78.675	0.0	0.0	0.0
30	0.0	79.025	0.0	0.0	0.0
31	0.0	79.7	0.0	0.0	0.0
32	0.0	80.7	0.0	0.0	0.0
33	0.0	81.8	0.0	0.0	0.0
34	0.0	83.275	0.0	0.0	0.0
35	0.0	83.95	0.0	0.0	0.0
36	0.0	84.475	0.0	0.0	0.0
37	0.0	85.125	0.0	0.0	0.0
38	0.0	85.475	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGA	20	6.952051E-4	44.000004	3
ACCGGTC	20	6.952051E-4	44.000004	10
AAGTAAT	40	5.315087E-9	44.000004	4
TCAAGTA	40	5.315087E-9	44.000004	2
AACCGGT	20	6.952051E-4	44.000004	9
AATCCAG	40	5.315087E-9	44.000004	8
TAAACAT	20	6.952051E-4	44.000004	3
ATCCAGG	40	5.315087E-9	44.000004	9
TTGTATA	20	6.952051E-4	44.000004	13
AAACATC	20	6.952051E-4	44.000004	4
AGTAGGT	20	6.952051E-4	44.000004	7
TTCAAGT	40	5.315087E-9	44.000004	1
AGGATAG	40	5.315087E-9	44.000004	13
GGTCTCT	20	6.952051E-4	44.000004	13
ATAGGCT	40	5.315087E-9	44.000004	16
TCCAGGA	40	5.315087E-9	44.000004	10
GTATAGT	20	6.952051E-4	44.000004	15
GGATAGG	40	5.315087E-9	44.000004	14
TAGTAGG	20	6.952051E-4	44.000004	6
AGTAATC	40	5.315087E-9	44.000004	5
>>END_MODULE
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282978 spots for SRR5030916.sra
Written 282978 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
Read 282971 spots for SRR5030916.sra
Written 282971 spots for SRR5030916.sra
SRR ids: ['SRR5030916.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_q4u02qyx
SRR5030916.sra spots: 3395659
blocks: [[1, 282971], [282972, 565942], [565943, 848913], [848914, 1131884], [1131885, 1414855], [1414856, 1697826], [1697827, 1980797], [1980798, 2263768], [2263769, 2546739], [2546740, 2829710], [2829711, 3112681], [3112682, 3395659]]
SRR5030916 file size 587466
SRR5030916 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030916 SRR5030916_1.fastq
Input file:	SRR5030916_1.fastq
trimmed:	SRR5030916-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:43:57 2020 >> started

Wed Mar  4 06:43:58 2020 >> done (1.880s)
3395659 reads processed; of these:
    117 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3395542 (100.00%) reads available; of these:
 199930 ( 5.89%) trimmed reads available after processing
3195612 (94.11%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     33	  0.00%
 19	     60	  0.00%
 20	    120	  0.00%
 21	    124	  0.00%
 22	    270	  0.01%
 23	    323	  0.01%
 24	    352	  0.01%
 25	    450	  0.01%
 26	    368	  0.01%
 27	    480	  0.01%
 28	    654	  0.02%
 29	   1014	  0.03%
 30	    730	  0.02%
 31	    773	  0.02%
 32	    811	  0.02%
 33	   1145	  0.03%
 34	   2764	  0.08%
 35	   2628	  0.08%
 36	   1767	  0.05%
 37	   2094	  0.06%
 38	   2329	  0.07%
 39	   2586	  0.08%
 40	   2938	  0.09%
 41	   3422	  0.10%
 42	   4602	  0.14%
 43	   5571	  0.16%
 44	  10247	  0.30%
 45	  14429	  0.42%
 46	  12491	  0.37%
 47	  18926	  0.56%
 48	  43106	  1.27%
 49	  62323	  1.84%
 50	3195612	 94.11%
3395542 reads passed initial QC


criterion=sequence-density
sequence-density=85.39
sequence-density-rank=1
fanout-score=17.75
fanout-score-rank=1
prefix-density=86.86
prefix-fanout=17.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC


criterion=fanout-score
sequence-density=85.39
sequence-density-rank=1
fanout-score=17.75
fanout-score-rank=1
prefix-density=86.86
prefix-fanout=17.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC -o SRR5030916 -
Input file:	STDIN
trimmed:	SRR5030916-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:44:14 2020 >> started

Wed Mar  4 06:44:20 2020 >> done (5.141s)
3316576 reads processed; of these:
 137699 ( 4.15%) short reads filtered out after trimming by size control
  10702 ( 0.32%) empty reads filtered out after trimming by size control
3168175 (95.53%) reads available; of these:
2901626 (91.59%) trimmed reads available after processing
 266549 ( 8.41%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  21578	  0.68%
 19	  37428	  1.18%
 20	 174472	  5.51%
 21	 384435	 12.13%
 22	1141147	 36.02%
 23	 503177	 15.88%
 24	 185083	  5.84%
 25	  32058	  1.01%
 26	  15532	  0.49%
 27	  14939	  0.47%
 28	  16355	  0.52%
 29	  12423	  0.39%
 30	  22600	  0.71%
 31	  27697	  0.87%
 32	  30274	  0.96%
 33	  49159	  1.55%
 34	  24685	  0.78%
 35	  15839	  0.50%
 36	  16915	  0.53%
 37	  12265	  0.39%
 38	  12931	  0.41%
 39	  13937	  0.44%
 40	  18388	  0.58%
 41	  25571	  0.81%
 42	  26354	  0.83%
 43	  19959	  0.63%
 44	  17375	  0.55%
 45	  19305	  0.61%
 46	  15534	  0.49%
 47	  12362	  0.39%
 48	   2887	  0.09%
 49	  10145	  0.32%
 50	 235366	  7.43%


criterion=sequence-density
sequence-density=8.43
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.51
sequence-density-rank=29
fanout-score=15.55
fanout-score-rank=1
prefix-density=7.89
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:44:41
                             Started mapping on |	Mar 04 06:44:42
                                    Finished on |	Mar 04 06:45:06
       Mapping speed, Million of reads per hour |	487.07

                          Number of input reads |	3247141
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1739959
                        Uniquely mapped reads % |	53.58%
                          Average mapped length |	26.19
                       Number of splices: Total |	1248
            Number of splices: Annotated (sjdb) |	160
                       Number of splices: GT/AG |	1105
                       Number of splices: GC/AG |	17
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	125
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1230977
             % of reads mapped to multiple loci |	37.91%
        Number of reads mapped to too many loci |	63199
             % of reads mapped to too many loci |	1.95%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.95%
                     % of reads unmapped: other |	0.61%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	276205	276205	276205
N_multimapping	1230977	1230977	1230977
N_noFeature	63168	77512	1307013
N_ambiguous	952448	585094	13766
UnstrandedReadsAssigned:724343 PositiveStrandReadsAssigned:1077353 NegativeStrandReadsAssigned:419180
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030916 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030916-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,247,141 reads, 706,246 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,128 rounds

 109283 SRR5030916.ke.tsv
  52636 SRR5030916.se.tsv
 161919 total
==> SRR5030916.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030916.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030916 completed mapping pipeline successfully
