Starting /dee2/code/volunteer_pipeline.sh SRR5030846
    current disk space = 160680460288
    free memory = 73962592 
SRR5030846 SRAfilesize
4d1394c91fe381d590a88746e97e0bb2  SRR5030846.sra
SRR5030846.sra file validated
SRR5030846 is single end
SRR5030846 is conventional basespace
SRR5030846 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4305	34.0	31.0	34.0	31.0	34.0
2	32.654	34.0	31.0	34.0	31.0	34.0
3	32.80425	34.0	31.0	34.0	31.0	34.0
4	36.192	37.0	37.0	37.0	35.0	37.0
5	36.226	37.0	37.0	37.0	35.0	37.0
6	36.1465	37.0	36.0	37.0	35.0	37.0
7	36.07375	37.0	35.0	37.0	35.0	37.0
8	36.149	37.0	36.0	37.0	35.0	37.0
9	37.94925	39.0	38.0	39.0	35.0	39.0
10	37.92875	39.0	38.0	39.0	35.0	39.0
11	37.89375	39.0	38.0	39.0	35.0	39.0
12	37.872	39.0	38.0	39.0	35.0	39.0
13	37.81225	39.0	38.0	39.0	35.0	39.0
14	39.25375	41.0	39.0	41.0	36.0	41.0
15	39.10075	41.0	39.0	41.0	35.0	41.0
16	39.23175	40.0	39.0	41.0	36.0	41.0
17	39.36075	41.0	39.0	41.0	36.0	41.0
18	39.29625	41.0	39.0	41.0	36.0	41.0
19	39.06475	41.0	39.0	41.0	36.0	41.0
20	39.033	40.0	39.0	41.0	35.0	41.0
21	39.16575	40.0	39.0	41.0	36.0	41.0
22	39.0485	41.0	39.0	41.0	35.0	41.0
23	38.869	40.0	39.0	41.0	35.0	41.0
24	38.6685	40.0	38.0	41.0	34.0	41.0
25	38.95975	41.0	39.0	41.0	35.0	41.0
26	39.0095	41.0	39.0	41.0	35.0	41.0
27	39.07925	41.0	39.0	41.0	36.0	41.0
28	38.7225	40.0	38.0	41.0	35.0	41.0
29	38.00275	40.0	38.0	41.0	33.0	41.0
30	38.2925	40.0	38.0	41.0	33.0	41.0
31	38.63775	40.0	38.0	41.0	34.0	41.0
32	38.798	40.0	38.0	41.0	35.0	41.0
33	37.9845	40.0	38.0	41.0	33.0	41.0
34	38.04	40.0	38.0	41.0	33.0	41.0
35	37.77175	40.0	37.0	41.0	33.0	41.0
36	34.32475	38.0	31.0	40.0	23.0	41.0
37	35.6385	38.0	35.0	40.0	28.0	41.0
38	36.86775	39.0	36.0	40.0	31.0	41.0
39	37.0455	39.0	37.0	40.0	31.0	41.0
40	36.961	39.0	36.0	41.0	31.0	41.0
41	37.0915	39.0	36.0	41.0	31.0	41.0
42	37.30725	40.0	37.0	41.0	32.0	41.0
43	37.127	40.0	37.0	41.0	32.0	41.0
44	36.95425	39.0	36.0	41.0	32.0	41.0
45	36.70375	39.0	36.0	40.0	31.0	41.0
46	35.0375	38.0	34.0	40.0	26.0	41.0
47	35.7065	39.0	35.0	40.0	28.0	41.0
48	36.1255	39.0	35.0	41.0	30.0	41.0
49	34.8415	38.0	34.0	40.0	27.0	41.0
50	30.71025	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	1.0
15	3.0
16	2.0
17	2.0
18	2.0
19	1.0
20	4.0
21	5.0
22	7.0
23	7.0
24	10.0
25	23.0
26	15.0
27	26.0
28	27.0
29	45.0
30	46.0
31	61.0
32	86.0
33	119.0
34	157.0
35	195.0
36	327.0
37	481.0
38	982.0
39	1362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.030015007503753	23.13656828414207	51.27563781890946	15.557778889444723
2	22.575	26.674999999999997	19.525000000000002	31.225
3	25.424999999999997	20.375	22.925	31.275
4	30.475	28.15	14.05	27.325
5	19.825	42.5	24.0	13.675
6	33.125	17.349999999999998	35.75	13.775
7	17.349999999999998	19.650000000000002	25.8	37.2
8	25.174999999999997	31.324999999999996	13.5	30.0
9	23.45	32.6	29.4	14.549999999999999
10	23.175	20.349999999999998	29.875	26.6
11	28.799999999999997	13.675	16.025	41.5
12	14.774999999999999	19.400000000000002	31.5	34.325
13	30.95	19.225	28.9	20.925
14	30.325000000000003	31.15	19.675	18.85
15	32.875	24.875	26.35	15.9
16	17.45	23.325000000000003	20.45	38.775
17	23.35	19.825	41.449999999999996	15.375
18	24.3	18.65	31.95	25.1
19	37.65	15.375	28.675	18.3
20	42.125	17.175	26.650000000000002	14.05
21	22.275	20.0	27.85	29.875
22	28.925	14.224999999999998	44.975	11.875
23	26.700000000000003	13.950000000000001	42.875	16.475
24	48.949999999999996	15.75	25.95	9.35
25	48.25	24.6	14.975	12.174999999999999
26	28.499999999999996	44.725	14.35	12.425
27	17.599999999999998	45.725	23.95	12.725
28	13.025	25.25	46.375	15.35
29	10.825	14.325	55.725	19.125
30	11.125	7.9750000000000005	35.65	45.25
31	15.925	6.15	45.975	31.95
32	27.775	7.825	21.175	43.225
33	52.7	9.025	15.225	23.05
34	58.525000000000006	8.75	16.775000000000002	15.950000000000001
35	62.4	8.3	17.875	11.425
36	42.825	10.2	32.425	14.549999999999999
37	43.875	11.875	20.549999999999997	23.7
38	23.0	16.825000000000003	13.900000000000002	46.275
39	17.474999999999998	24.15	9.85	48.525
40	15.85	45.925	12.7	25.525
41	25.0	46.800000000000004	10.549999999999999	17.65
42	48.65	30.5	11.225	9.625
43	48.8	31.624999999999996	10.2	9.375
44	29.725	48.05	8.475000000000001	13.750000000000002
45	18.224999999999998	49.825	13.725000000000001	18.224999999999998
46	11.475	29.2	19.2	40.125
47	8.924999999999999	16.85	38.824999999999996	35.4
48	10.2	13.750000000000002	21.3	54.75
49	13.8	19.975	15.475	50.74999999999999
50	17.775	36.225	14.6	31.4
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	1.0
25	1.5
26	2.0
27	3.0
28	4.0
29	4.0
30	4.0
31	12.0
32	20.0
33	25.0
34	30.0
35	39.5
36	49.0
37	61.0
38	73.0
39	67.0
40	61.0
41	52.0
42	43.0
43	74.0
44	105.0
45	169.5
46	234.0
47	315.0
48	396.0
49	502.0
50	608.0
51	698.5
52	789.0
53	617.0
54	445.0
55	487.5
56	530.0
57	397.5
58	265.0
59	168.5
60	72.0
61	59.5
62	47.0
63	74.5
64	102.0
65	68.0
66	34.0
67	27.0
68	20.0
69	17.5
70	15.0
71	24.0
72	33.0
73	18.5
74	4.0
75	4.5
76	5.0
77	4.0
78	3.0
79	2.5
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86204782342122	30.525000000000002
2	10.177805027590434	8.3
3	4.782342121397916	5.8500000000000005
4	1.900674432863274	3.1
5	1.594114040465972	3.25
6	1.1036174126302882	2.7
7	0.6744328632740649	1.925
8	0.9196811771919068	3.0
9	0.6744328632740649	2.475
>10	2.8816676885346415	22.975
>50	0.2452483139178418	7.75
>100	0.18393623543838136	8.15
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	114	2.85	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	109	2.725	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	88	2.1999999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	77	1.925	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	62	1.55	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	44	1.0999999999999999	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	42	1.05	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT	31	0.775	RNA PCR Primer, Index 1 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTC	22	0.5499999999999999	RNA PCR Primer, Index 39 (100% over 44bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	21	0.525	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	19	0.475	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	15	0.375	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	12	0.3	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	12	0.3	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	10	0.25	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	10	0.25	RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	10	0.25	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	9	0.22499999999999998	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	9	0.22499999999999998	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	9	0.22499999999999998	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	8	0.2	No Hit
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	8	0.2	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	7	0.17500000000000002	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 39 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	6	0.15	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	6	0.15	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	6	0.15	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA	5	0.125	RNA PCR Primer, Index 2 (97% over 36bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	5	0.125	RNA PCR Primer, Index 1 (96% over 29bp)
GACCCTGTTGATCTCAAATACTAATTGCCAGTTTTGTCTGATGTGGAATT	5	0.125	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.225	0.0	0.0	0.0
2	0.0	0.225	0.0	0.0	0.0
3	0.0	0.375	0.0	0.0	0.0
4	0.0	0.375	0.0	0.0	0.0
5	0.0	0.375	0.0	0.0	0.0
6	0.0	0.45	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.35	0.0	0.0	0.0
9	0.0	1.475	0.0	0.0	0.0
10	0.0	1.55	0.0	0.0	0.0
11	0.0	1.7	0.0	0.0	0.0
12	0.0	1.975	0.0	0.0	0.0
13	0.0	2.275	0.0	0.0	0.0
14	0.0	2.55	0.0	0.0	0.0
15	0.0	2.975	0.0	0.0	0.0
16	0.0	4.075	0.0	0.0	0.0
17	0.0	4.7	0.0	0.0	0.0
18	0.0	5.875	0.0	0.0	0.0
19	0.0	6.65	0.0	0.0	0.0
20	0.0	7.45	0.0	0.0	0.0
21	0.0	13.075	0.0	0.0	0.0
22	0.0	23.7	0.0	0.0	0.0
23	0.0	51.5	0.0	0.0	0.0
24	0.0	63.425	0.0	0.0	0.0
25	0.0	69.825	0.0	0.0	0.0
26	0.0	71.1	0.0	0.0	0.0
27	0.0	71.825	0.0	0.0	0.0
28	0.0	72.3	0.0	0.0	0.0
29	0.0	72.725	0.0	0.0	0.0
30	0.0	72.95	0.0	0.0	0.0
31	0.0	73.6	0.0	0.0	0.0
32	0.0	74.5	0.0	0.0	0.0
33	0.0	75.45	0.0	0.0	0.0
34	0.0	77.6	0.0	0.0	0.0
35	0.0	78.3	0.0	0.0	0.0
36	0.0	78.725	0.0	0.0	0.0
37	0.0	79.375	0.0	0.0	0.0
38	0.0	79.8	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
GGTGAGT	20	6.952051E-4	44.000004	17
ACGCTGT	20	6.952051E-4	44.000004	9
TAAACAT	20	6.952051E-4	44.000004	3
AAACATC	20	6.952051E-4	44.000004	4
TTCAACG	20	6.952051E-4	44.000004	5
TCGGTGA	20	6.952051E-4	44.000004	15
CATTCAA	20	6.952051E-4	44.000004	3
TCAACGC	20	6.952051E-4	44.000004	6
ACATTCA	20	6.952051E-4	44.000004	2
GCTGTCG	20	6.952051E-4	44.000004	11
GGCTTGG	20	6.952051E-4	44.000004	19
CGGTGAG	20	6.952051E-4	44.000004	16
GGTGGAA	20	6.952051E-4	44.000004	19
CTGTCGG	20	6.952051E-4	44.000004	12
TGGTGGA	20	6.952051E-4	44.000004	18
ATTCAAC	20	6.952051E-4	44.000004	4
GTCGGTG	20	6.952051E-4	44.000004	14
AACATTC	20	6.952051E-4	44.000004	1
TGTCGGT	20	6.952051E-4	44.000004	13
>>END_MODULE
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
Read 239354 spots for SRR5030846.sra
Written 239354 spots for SRR5030846.sra
Read 239352 spots for SRR5030846.sra
Written 239352 spots for SRR5030846.sra
SRR ids: ['SRR5030846.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bdvns9gx
SRR5030846.sra spots: 2872226
blocks: [[1, 239352], [239353, 478704], [478705, 718056], [718057, 957408], [957409, 1196760], [1196761, 1436112], [1436113, 1675464], [1675465, 1914816], [1914817, 2154168], [2154169, 2393520], [2393521, 2632872], [2632873, 2872226]]
SRR5030846 file size 496745
SRR5030846 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030846 SRR5030846_1.fastq
Input file:	SRR5030846_1.fastq
trimmed:	SRR5030846-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:35 2020 >> started

Wed Mar  4 06:26:37 2020 >> done (1.654s)
2872226 reads processed; of these:
    116 ( 0.00%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
2872106 (100.00%) reads available; of these:
 163625 ( 5.70%) trimmed reads available after processing
2708481 (94.30%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     45	  0.00%
 19	     61	  0.00%
 20	    124	  0.00%
 21	    149	  0.01%
 22	    267	  0.01%
 23	    347	  0.01%
 24	    342	  0.01%
 25	    469	  0.02%
 26	    401	  0.01%
 27	    559	  0.02%
 28	    703	  0.02%
 29	   1073	  0.04%
 30	    760	  0.03%
 31	    855	  0.03%
 32	   1045	  0.04%
 33	   1355	  0.05%
 34	   2407	  0.08%
 35	   2482	  0.09%
 36	   1907	  0.07%
 37	   2108	  0.07%
 38	   2207	  0.08%
 39	   2563	  0.09%
 40	   2743	  0.10%
 41	   3234	  0.11%
 42	   3955	  0.14%
 43	   5636	  0.20%
 44	   9281	  0.32%
 45	  11289	  0.39%
 46	  10876	  0.38%
 47	  15840	  0.55%
 48	  34001	  1.18%
 49	  44541	  1.55%
 50	2708481	 94.30%
2872106 reads passed initial QC


criterion=sequence-density
sequence-density=80.50
sequence-density-rank=1
fanout-score=19.78
fanout-score-rank=1
prefix-density=82.50
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC


criterion=fanout-score
sequence-density=80.50
sequence-density-rank=1
fanout-score=19.78
fanout-score-rank=1
prefix-density=82.50
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC -o SRR5030846 -
Input file:	STDIN
trimmed:	SRR5030846-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTATACATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:26:51 2020 >> started

Wed Mar  4 06:26:54 2020 >> done (3.027s)
2801190 reads processed; of these:
 165846 ( 5.92%) short reads filtered out after trimming by size control
   9050 ( 0.32%) empty reads filtered out after trimming by size control
2626294 (93.76%) reads available; of these:
2336706 (88.97%) trimmed reads available after processing
 289588 (11.03%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  18392	  0.70%
 19	  28099	  1.07%
 20	 155831	  5.93%
 21	 322574	 12.28%
 22	 775801	 29.54%
 23	 335967	 12.79%
 24	 168314	  6.41%
 25	  45994	  1.75%
 26	  23175	  0.88%
 27	  17381	  0.66%
 28	  17805	  0.68%
 29	  13280	  0.51%
 30	  20041	  0.76%
 31	  24085	  0.92%
 32	  24648	  0.94%
 33	  59546	  2.27%
 34	  22699	  0.86%
 35	  19878	  0.76%
 36	  21387	  0.81%
 37	  13522	  0.51%
 38	  14311	  0.54%
 39	  15834	  0.60%
 40	  23007	  0.88%
 41	  30958	  1.18%
 42	  34667	  1.32%
 43	  25278	  0.96%
 44	  21316	  0.81%
 45	  28008	  1.07%
 46	  20496	  0.78%
 47	  14885	  0.57%
 48	   3052	  0.12%
 49	   9977	  0.38%
 50	 256086	  9.75%


criterion=sequence-density
sequence-density=5.17
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=19
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=39
fanout-score=68.27
fanout-score-rank=1
prefix-density=1.08
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 06:28:06
                             Started mapping on |	Mar 04 06:28:06
                                    Finished on |	Mar 04 06:28:29
       Mapping speed, Million of reads per hour |	422.17

                          Number of input reads |	2697210
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1577929
                        Uniquely mapped reads % |	58.50%
                          Average mapped length |	27.11
                       Number of splices: Total |	1071
            Number of splices: Annotated (sjdb) |	137
                       Number of splices: GT/AG |	982
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	75
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.55
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	803101
             % of reads mapped to multiple loci |	29.78%
        Number of reads mapped to too many loci |	63846
             % of reads mapped to too many loci |	2.37%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.52%
                     % of reads unmapped: other |	0.83%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	316180	316180	316180
N_multimapping	803101	803101	803101
N_noFeature	64229	82936	1085770
N_ambiguous	889622	467101	19631
UnstrandedReadsAssigned:624078 PositiveStrandReadsAssigned:1027892 NegativeStrandReadsAssigned:472528
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=21 echo kmer=19
SRR5030846 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030846-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,697,210 reads, 750,473 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,106 rounds

 109283 SRR5030846.ke.tsv
  52636 SRR5030846.se.tsv
 161919 total
==> SRR5030846.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030846.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030846 completed mapping pipeline successfully
