Starting /dee2/code/volunteer_pipeline.sh SRR5030890
    current disk space = 160630325248
    free memory = 73947168 
SRR5030890 SRAfilesize
b5b94fdcd4d16d3fe80ce25d64288190  SRR5030890.sra
SRR5030890.sra file validated
SRR5030890 is single end
SRR5030890 is conventional basespace
SRR5030890 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.406	33.0	31.0	34.0	31.0	34.0
2	32.7105	34.0	31.0	34.0	31.0	34.0
3	32.7965	34.0	31.0	34.0	31.0	34.0
4	36.24025	37.0	37.0	37.0	35.0	37.0
5	36.20125	37.0	37.0	37.0	35.0	37.0
6	35.96675	37.0	35.0	37.0	35.0	37.0
7	36.10525	37.0	35.0	37.0	35.0	37.0
8	36.14475	37.0	35.0	37.0	35.0	37.0
9	37.89775	39.0	38.0	39.0	35.0	39.0
10	37.867	39.0	38.0	39.0	35.0	39.0
11	37.771	39.0	38.0	39.0	35.0	39.0
12	37.86025	39.0	38.0	39.0	35.0	39.0
13	37.7665	39.0	38.0	39.0	35.0	39.0
14	39.115	41.0	39.0	41.0	36.0	41.0
15	39.1875	40.0	39.0	41.0	36.0	41.0
16	39.094	40.0	39.0	41.0	36.0	41.0
17	39.2375	41.0	39.0	41.0	36.0	41.0
18	39.221	41.0	39.0	41.0	36.0	41.0
19	38.901	40.0	38.0	41.0	35.0	41.0
20	38.9745	40.0	39.0	41.0	35.0	41.0
21	38.958	40.0	39.0	41.0	35.0	41.0
22	39.00125	40.0	39.0	41.0	35.0	41.0
23	38.945	40.0	39.0	41.0	35.0	41.0
24	38.15525	40.0	38.0	41.0	33.0	41.0
25	38.42125	40.0	38.0	41.0	34.0	41.0
26	38.334	40.0	38.0	41.0	34.0	41.0
27	38.44175	40.0	38.0	41.0	34.0	41.0
28	38.47525	40.0	38.0	41.0	34.0	41.0
29	38.33275	40.0	38.0	41.0	33.0	41.0
30	38.081	40.0	38.0	41.0	33.0	41.0
31	38.53075	40.0	38.0	41.0	34.0	41.0
32	38.75525	40.0	38.0	41.0	35.0	41.0
33	37.11525	40.0	37.0	41.0	30.0	41.0
34	36.45625	40.0	36.0	41.0	29.0	41.0
35	33.56175	38.0	32.0	40.0	16.0	41.0
36	30.05675	37.0	23.0	40.0	8.0	41.0
37	30.4695	36.0	25.0	39.0	8.0	41.0
38	32.78775	37.0	31.0	40.0	20.0	41.0
39	32.20175	37.0	30.0	40.0	9.0	41.0
40	33.00625	38.0	32.0	40.0	11.0	41.0
41	30.945	37.0	28.0	40.0	7.0	41.0
42	32.393	37.0	31.0	40.0	2.0	41.0
43	30.9575	37.0	28.0	40.0	2.0	41.0
44	31.733	38.0	31.0	40.0	2.0	41.0
45	30.01375	37.0	27.0	40.0	2.0	41.0
46	27.8775	35.0	18.0	39.0	2.0	41.0
47	27.8265	35.0	22.0	39.0	2.0	41.0
48	28.5145	35.0	24.0	39.0	2.0	41.0
49	26.69425	35.0	2.0	39.0	2.0	41.0
50	23.13025	31.0	2.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	1.0
17	1.0
18	3.0
19	6.0
20	15.0
21	20.0
22	22.0
23	25.0
24	51.0
25	47.0
26	73.0
27	126.0
28	77.0
29	109.0
30	144.0
31	156.0
32	163.0
33	185.0
34	222.0
35	266.0
36	343.0
37	490.0
38	676.0
39	776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.685263947960971	24.49337002752064	44.183137353014764	17.63822867150363
2	26.974999999999998	25.825	20.349999999999998	26.85
3	22.075	20.7	21.925	35.3
4	30.4	28.249999999999996	16.400000000000002	24.95
5	21.925	38.574999999999996	26.575	12.925
6	30.925000000000004	15.25	37.3	16.525000000000002
7	19.8	21.475	23.724999999999998	35.0
8	26.275	29.925	17.849999999999998	25.95
9	19.825	33.4	30.625000000000004	16.150000000000002
10	24.325	21.85	27.05	26.775
11	37.325	13.825000000000001	15.5	33.35
12	19.175	21.475	26.575	32.775
13	25.374999999999996	19.15	36.225	19.25
14	28.499999999999996	28.025	23.0	20.474999999999998
15	32.6	23.65	26.474999999999998	17.275
16	19.75	20.575	24.75	34.925
17	26.075	25.074999999999996	33.550000000000004	15.299999999999999
18	25.2	17.4	28.675	28.725
19	34.025	15.725	31.874999999999996	18.375
20	39.0	16.225	29.375	15.4
21	26.625	21.224999999999998	27.575	24.575
22	30.75	13.55	42.4	13.3
23	26.424999999999997	14.799999999999999	41.0	17.775
24	48.6	14.05	25.15	12.2
25	46.025	23.724999999999998	16.1	14.149999999999999
26	28.325	40.400000000000006	15.375	15.9
27	16.625	42.85	22.375	18.15
28	14.075	25.0	43.7	17.224999999999998
29	14.75	13.925	51.575	19.75
30	12.325	8.15	35.0	44.525
31	14.924999999999999	7.5	44.324999999999996	33.25
32	30.0	6.625	23.200000000000003	40.175
33	47.949999999999996	12.15	15.024999999999999	24.875
34	55.925000000000004	8.674999999999999	16.725	18.675
35	57.599999999999994	8.450000000000001	20.549999999999997	13.4
36	58.525000000000006	9.425	18.4	13.65
37	37.625	14.725	22.5	25.15
38	25.650000000000002	16.925	14.075	43.35
39	17.925	24.4	13.350000000000001	44.324999999999996
40	16.900000000000002	44.05	14.774999999999999	24.275
41	26.775	43.1	12.0	18.125
42	46.35	28.9	13.725000000000001	11.025
43	43.925	31.574999999999996	11.525	12.975
44	30.725	44.074999999999996	10.375	14.825
45	21.525	43.85	11.200000000000001	23.425
46	15.9	27.875	23.125	33.1
47	11.975	16.85	34.625	36.55
48	15.049999999999999	12.3	22.575	50.075
49	15.375	23.724999999999998	14.35	46.550000000000004
50	28.375	26.400000000000002	11.450000000000001	33.775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.5
28	3.0
29	3.0
30	3.0
31	8.5
32	14.0
33	18.0
34	22.0
35	34.0
36	46.0
37	74.5
38	103.0
39	96.5
40	90.0
41	83.5
42	77.0
43	95.0
44	113.0
45	154.0
46	195.0
47	244.0
48	293.0
49	372.5
50	452.0
51	629.0
52	806.0
53	638.0
54	470.0
55	432.0
56	394.0
57	346.5
58	299.0
59	225.0
60	151.0
61	120.0
62	89.0
63	139.0
64	189.0
65	115.5
66	42.0
67	30.0
68	18.0
69	20.0
70	22.0
71	51.5
72	81.0
73	44.0
74	7.0
75	5.0
76	3.0
77	3.5
78	4.0
79	5.0
80	6.0
81	6.0
82	6.0
83	3.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.275000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.35803083043263	40.400000000000006
2	7.956240676280457	8.0
3	3.3316757831924413	5.025
4	1.8398806563898558	3.6999999999999997
5	0.9945300845350571	2.5
6	0.9448035803083044	2.85
7	0.5469915464942815	1.925
8	0.49726504226752855	2.0
9	0.69617105917454	3.15
>10	2.6852312282446547	23.200000000000003
>50	0.04972650422675286	1.55
>100	0.09945300845350571	5.7
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	123	3.075	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	105	2.625	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	62	1.55	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	36	0.8999999999999999	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	33	0.8250000000000001	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	26	0.65	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	24	0.6	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	21	0.525	RNA PCR Primer, Index 7 (100% over 35bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	21	0.525	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	21	0.525	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC	19	0.475	RNA PCR Primer, Index 7 (100% over 50bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	16	0.4	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	15	0.375	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	14	0.35000000000000003	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC	14	0.35000000000000003	RNA PCR Primer, Index 7 (100% over 44bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	13	0.325	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	12	0.3	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 33bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	11	0.27499999999999997	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	11	0.27499999999999997	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	10	0.25	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	10	0.25	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	10	0.25	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	10	0.25	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCG	10	0.25	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	10	0.25	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	9	0.22499999999999998	RNA PCR Primer, Index 2 (100% over 34bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	9	0.22499999999999998	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	8	0.2	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	8	0.2	No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	8	0.2	RNA PCR Primer, Index 1 (96% over 29bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT	7	0.17500000000000002	RNA PCR Primer, Index 7 (100% over 38bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 30bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	6	0.15	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAATTCCG	6	0.15	RNA PCR Primer, Index 1 (95% over 23bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	6	0.15	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	6	0.15	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	6	0.15	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	6	0.15	RNA PCR Primer, Index 1 (100% over 32bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	6	0.15	No Hit
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCC	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	6	0.15	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	6	0.15	No Hit
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC	5	0.125	RNA PCR Primer, Index 7 (100% over 44bp)
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.55	0.0	0.0	0.0
2	0.0	0.55	0.0	0.0	0.0
3	0.0	0.6	0.0	0.0	0.0
4	0.0	0.625	0.0	0.0	0.0
5	0.0	0.65	0.0	0.0	0.0
6	0.0	0.75	0.0	0.0	0.0
7	0.0	1.225	0.0	0.0	0.0
8	0.0	1.45	0.0	0.0	0.0
9	0.0	1.475	0.0	0.0	0.0
10	0.0	1.675	0.0	0.0	0.0
11	0.0	1.825	0.0	0.0	0.0
12	0.0	1.95	0.0	0.0	0.0
13	0.0	2.325	0.0	0.0	0.0
14	0.0	2.525	0.0	0.0	0.0
15	0.0	2.825	0.0	0.0	0.0
16	0.0	3.775	0.0	0.0	0.0
17	0.0	4.3	0.0	0.0	0.0
18	0.0	5.075	0.0	0.0	0.0
19	0.0	5.5	0.0	0.0	0.0
20	0.0	6.7	0.0	0.0	0.0
21	0.0	10.5	0.0	0.0	0.0
22	0.0	19.975	0.0	0.0	0.0
23	0.0	44.25	0.0	0.0	0.0
24	0.0	56.025	0.0	0.0	0.0
25	0.0	61.025	0.0	0.0	0.0
26	0.0	62.05	0.0	0.0	0.0
27	0.0	62.7	0.0	0.0	0.0
28	0.0	63.15	0.0	0.0	0.0
29	0.0	63.4	0.0	0.0	0.0
30	0.0	63.925	0.0	0.0	0.0
31	0.0	64.75	0.0	0.0	0.0
32	0.0	65.5	0.0	0.0	0.0
33	0.0	66.125	0.0	0.0	0.0
34	0.0	70.2	0.0	0.0	0.0
35	0.0	71.5	0.0	0.0	0.0
36	0.0	72.05	0.0	0.0	0.0
37	0.0	73.025	0.0	0.0	0.0
38	0.0	73.65	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	20	6.952051E-4	44.000004	1
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AATCCAG	20	6.952051E-4	44.000004	8
ATCCAGG	20	6.952051E-4	44.000004	9
GCGACCT	20	6.952051E-4	44.000004	2
GCGACCC	20	6.952051E-4	44.000004	22
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
CCGCTGA	20	6.952051E-4	44.000004	27
ATAGGCT	20	6.952051E-4	44.000004	16
GAGCTTG	20	6.952051E-4	44.000004	13
GGATCCG	20	6.952051E-4	44.000004	3
GGCGACC	20	6.952051E-4	44.000004	21
TCGGATC	20	6.952051E-4	44.000004	1
ACCCGCT	20	6.952051E-4	44.000004	25
CGGATCC	20	6.952051E-4	44.000004	2
GGATAGG	20	6.952051E-4	44.000004	14
CCCGCTG	20	6.952051E-4	44.000004	26
CGTGGCG	20	6.952051E-4	44.000004	18
>>END_MODULE
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237805 spots for SRR5030890.sra
Written 237805 spots for SRR5030890.sra
Read 237807 spots for SRR5030890.sra
Written 237807 spots for SRR5030890.sra
SRR ids: ['SRR5030890.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_2dzy2gbz
SRR5030890.sra spots: 2853662
blocks: [[1, 237805], [237806, 475610], [475611, 713415], [713416, 951220], [951221, 1189025], [1189026, 1426830], [1426831, 1664635], [1664636, 1902440], [1902441, 2140245], [2140246, 2378050], [2378051, 2615855], [2615856, 2853662]]
SRR5030890 file size 493524
SRR5030890 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030890 SRR5030890_1.fastq
Input file:	SRR5030890_1.fastq
trimmed:	SRR5030890-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:38:08 2020 >> started

Wed Mar  4 06:38:10 2020 >> done (1.688s)
2853662 reads processed; of these:
    174 ( 0.01%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
2853485 (99.99%) reads available; of these:
 322634 (11.31%) trimmed reads available after processing
2530851 (88.69%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     36	  0.00%
 19	     88	  0.00%
 20	    123	  0.00%
 21	    208	  0.01%
 22	    331	  0.01%
 23	    453	  0.02%
 24	    473	  0.02%
 25	    604	  0.02%
 26	    447	  0.02%
 27	    609	  0.02%
 28	    856	  0.03%
 29	   1153	  0.04%
 30	    886	  0.03%
 31	   1260	  0.04%
 32	   3041	  0.11%
 33	   9293	  0.33%
 34	  20858	  0.73%
 35	   5240	  0.18%
 36	   5865	  0.21%
 37	   3340	  0.12%
 38	   6499	  0.23%
 39	   6341	  0.22%
 40	  11977	  0.42%
 41	   7076	  0.25%
 42	  15749	  0.55%
 43	   7532	  0.26%
 44	  29204	  1.02%
 45	  20462	  0.72%
 46	  19618	  0.69%
 47	  20371	  0.71%
 48	  57380	  2.01%
 49	  65261	  2.29%
 50	2530851	 88.69%
2853485 reads passed initial QC


criterion=sequence-density
sequence-density=72.78
sequence-density-rank=1
fanout-score=22.27
fanout-score-rank=3
prefix-density=75.34
prefix-fanout=21.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC


criterion=fanout-score
sequence-density=1.23
sequence-density-rank=7
fanout-score=595.63
fanout-score-rank=1
prefix-density=74.53
prefix-fanout=9.8
sequence=GGAATTCTCGGTGCCAAGGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC -o SRR5030890 -
Input file:	STDIN
trimmed:	SRR5030890-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:38:25 2020 >> started

Wed Mar  4 06:38:29 2020 >> done (3.815s)
2775307 reads processed; of these:
 136622 ( 4.92%) short reads filtered out after trimming by size control
  18452 ( 0.66%) empty reads filtered out after trimming by size control
2620233 (94.41%) reads available; of these:
2134807 (81.47%) trimmed reads available after processing
 485426 (18.53%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15439	  0.59%
 19	  24648	  0.94%
 20	 102236	  3.90%
 21	 249460	  9.52%
 22	 657494	 25.09%
 23	 338343	 12.91%
 24	 147140	  5.62%
 25	  39985	  1.53%
 26	  21137	  0.81%
 27	  18339	  0.70%
 28	  19884	  0.76%
 29	  17214	  0.66%
 30	  21129	  0.81%
 31	  25552	  0.98%
 32	  27360	  1.04%
 33	 115137	  4.39%
 34	  33402	  1.27%
 35	  24874	  0.95%
 36	  35833	  1.37%
 37	  15124	  0.58%
 38	  17684	  0.67%
 39	  17016	  0.65%
 40	  24903	  0.95%
 41	  29894	  1.14%
 42	  36392	  1.39%
 43	  25166	  0.96%
 44	  22713	  0.87%
 45	  27017	  1.03%
 46	  23128	  0.88%
 47	  19384	  0.74%
 48	   7267	  0.28%
 49	  13098	  0.50%
 50	 406841	 15.53%


criterion=sequence-density
sequence-density=6.72
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.45
sequence-density-rank=28
fanout-score=11.53
fanout-score-rank=1
prefix-density=5.19
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:38:50
                             Started mapping on |	Mar 04 06:38:50
                                    Finished on |	Mar 04 06:39:13
       Mapping speed, Million of reads per hour |	422.36

                          Number of input reads |	2698411
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1356493
                        Uniquely mapped reads % |	50.27%
                          Average mapped length |	29.26
                       Number of splices: Total |	1821
            Number of splices: Annotated (sjdb) |	250
                       Number of splices: GT/AG |	1697
                       Number of splices: GC/AG |	21
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	100
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.33
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	856107
             % of reads mapped to multiple loci |	31.73%
        Number of reads mapped to too many loci |	72368
             % of reads mapped to too many loci |	2.68%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.74%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	485811	485811	485811
N_multimapping	856107	856107	856107
N_noFeature	72266	89193	1026569
N_ambiguous	736646	456403	14434
UnstrandedReadsAssigned:547581 PositiveStrandReadsAssigned:810897 NegativeStrandReadsAssigned:315490
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030890 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030890-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,698,411 reads, 599,793 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,112 rounds

 109283 SRR5030890.ke.tsv
  52636 SRR5030890.se.tsv
 161919 total
==> SRR5030890.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030890.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030890 completed mapping pipeline successfully
