Starting /dee2/code/volunteer_pipeline.sh SRR5031086
    current disk space = 160488980480
    free memory = 73909108 
SRR5031086 SRAfilesize
7ce6dfbf3fc6ed687d55ac9c71ced899  SRR5031086.sra
SRR5031086.sra file validated
SRR5031086 is single end
SRR5031086 is conventional basespace
SRR5031086 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.162	33.0	31.0	34.0	30.0	34.0
2	32.559	34.0	31.0	34.0	31.0	34.0
3	32.47825	34.0	31.0	34.0	31.0	34.0
4	36.1815	37.0	35.0	37.0	35.0	37.0
5	36.16475	37.0	35.0	37.0	35.0	37.0
6	36.0515	37.0	35.0	37.0	35.0	37.0
7	35.94775	37.0	35.0	37.0	35.0	37.0
8	35.8315	37.0	35.0	37.0	35.0	37.0
9	37.273	39.0	37.0	39.0	34.0	39.0
10	37.056	39.0	37.0	39.0	33.0	39.0
11	37.63275	39.0	37.0	39.0	35.0	39.0
12	37.85325	39.0	37.0	39.0	35.0	39.0
13	37.5935	39.0	37.0	39.0	35.0	39.0
14	38.59175	40.0	38.0	41.0	34.0	41.0
15	37.749	40.0	37.0	41.0	32.0	41.0
16	37.19275	39.0	37.0	40.0	32.0	41.0
17	36.95475	39.0	36.0	41.0	31.0	41.0
18	38.23825	40.0	38.0	41.0	34.0	41.0
19	38.792	40.0	38.0	41.0	36.0	41.0
20	38.43825	40.0	38.0	41.0	35.0	41.0
21	38.7775	40.0	38.0	41.0	35.0	41.0
22	37.6965	40.0	37.0	41.0	32.0	41.0
23	38.08175	39.0	38.0	40.0	34.0	41.0
24	37.83025	40.0	37.0	41.0	33.0	41.0
25	38.4705	40.0	38.0	41.0	34.0	41.0
26	38.306	40.0	38.0	41.0	34.0	41.0
27	38.50775	40.0	38.0	41.0	34.0	41.0
28	38.63825	40.0	38.0	41.0	34.0	41.0
29	38.80175	40.0	39.0	41.0	35.0	41.0
30	38.925	40.0	38.0	41.0	35.0	41.0
31	38.96375	40.0	38.0	41.0	35.0	41.0
32	38.9575	40.0	38.0	41.0	35.0	41.0
33	38.8345	40.0	38.0	41.0	35.0	41.0
34	38.46125	40.0	38.0	41.0	34.0	41.0
35	37.753	40.0	37.0	41.0	31.0	41.0
36	33.041	35.0	30.0	40.0	23.0	41.0
37	34.93025	37.0	33.0	39.0	30.0	40.0
38	36.4555	38.0	35.0	39.0	30.0	40.0
39	37.01925	38.0	37.0	40.0	33.0	40.0
40	37.4515	39.0	37.0	40.0	33.0	41.0
41	37.74525	40.0	38.0	40.0	33.0	41.0
42	37.732	40.0	37.0	41.0	33.0	41.0
43	37.76225	40.0	38.0	41.0	33.0	41.0
44	36.9295	39.0	36.0	40.0	31.0	41.0
45	37.106	39.0	37.0	40.0	32.0	41.0
46	37.096	39.0	37.0	40.0	31.0	41.0
47	36.38975	39.0	35.0	40.0	30.0	41.0
48	37.31625	39.0	37.0	40.0	33.0	41.0
49	37.536	40.0	37.0	41.0	33.0	41.0
50	35.87125	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	1.0
16	1.0
17	0.0
18	1.0
19	0.0
20	4.0
21	5.0
22	3.0
23	4.0
24	7.0
25	7.0
26	10.0
27	15.0
28	21.0
29	32.0
30	44.0
31	60.0
32	77.0
33	122.0
34	166.0
35	231.0
36	434.0
37	699.0
38	1173.0
39	880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.705028771578685	25.068801601200903	57.39304478358769	10.833124843632724
2	27.81672508763145	28.11717576364547	22.70906359539309	21.357035553329993
3	17.925	19.525000000000002	20.3	42.25
4	27.650000000000002	44.6	10.174999999999999	17.575
5	20.9	40.325	29.175	9.6
6	31.05	16.1	41.875	10.975
7	11.725	20.95	26.424999999999997	40.9
8	21.15	44.5	13.600000000000001	20.75
9	16.8	39.0	32.175	12.025
10	17.875	19.15	27.025	35.949999999999996
11	35.725	8.275	14.924999999999999	41.075
12	16.725	14.075	25.0	44.2
13	22.375	20.25	39.225	18.15
14	44.05	19.0	24.8	12.15
15	41.55	15.6	29.025000000000002	13.825000000000001
16	13.125	21.675	22.2	43.0
17	28.7	17.5	39.900000000000006	13.900000000000002
18	34.425	19.6	25.650000000000002	20.325
19	40.275	15.5	31.5	12.725
20	55.425000000000004	16.075	18.375	10.125
21	20.4	27.750000000000004	24.8	27.05
22	33.75	10.95	47.475	7.825
23	22.875	12.9	52.300000000000004	11.924999999999999
24	54.025	9.375	28.925	7.675
25	58.425000000000004	20.075000000000003	15.4	6.1
26	30.25	50.5	11.5	7.75
27	13.775	55.45	21.25	9.525
28	8.7	27.725	52.87500000000001	10.7
29	10.549999999999999	10.2	61.324999999999996	17.925
30	8.450000000000001	4.375	39.925	47.25
31	11.275	3.5999999999999996	47.975	37.15
32	23.525	6.075	22.650000000000002	47.75
33	57.199999999999996	5.7250000000000005	11.85	25.224999999999998
34	71.075	5.75	12.0	11.175
35	70.35	7.449999999999999	15.25	6.950000000000001
36	45.75	6.15	38.775	9.325
37	47.925000000000004	8.5	20.925	22.650000000000002
38	23.549999999999997	11.899999999999999	12.55	52.0
39	12.15	21.0	8.525	58.325
40	10.7	54.0	6.550000000000001	28.749999999999996
41	20.65	58.425000000000004	7.049999999999999	13.875000000000002
42	52.37500000000001	32.2	5.7250000000000005	9.700000000000001
43	58.3	26.674999999999997	6.550000000000001	8.475000000000001
44	29.849999999999998	51.675000000000004	6.175	12.3
45	14.7	57.125	10.025	18.15
46	7.35	27.975	18.175	46.5
47	5.825	14.35	41.875	37.95
48	6.25	9.65	23.65	60.45
49	10.05	17.75	12.575	59.62499999999999
50	15.975	41.825	9.85	32.35
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	0.0
29	1.0
30	2.0
31	2.5
32	3.0
33	4.0
34	5.0
35	4.0
36	3.0
37	7.0
38	11.0
39	14.5
40	18.0
41	19.5
42	21.0
43	56.5
44	92.0
45	161.5
46	231.0
47	446.5
48	662.0
49	669.5
50	677.0
51	879.5
52	1082.0
53	742.5
54	403.0
55	356.5
56	310.0
57	279.0
58	248.0
59	167.5
60	87.0
61	58.5
62	30.0
63	35.0
64	40.0
65	30.0
66	20.0
67	14.5
68	9.0
69	9.0
70	9.0
71	20.0
72	31.0
73	17.5
74	4.0
75	2.0
76	0.0
77	0.0
78	0.0
79	0.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62797012898847	27.85
2	9.23285811269518	6.800000000000001
3	4.276985743380855	4.725
4	2.4439918533604885	3.5999999999999996
5	1.4256619144602851	2.625
6	1.1541072640868975	2.55
7	0.7467752885268161	1.925
8	0.47522063815342835	1.4000000000000001
9	0.40733197556008144	1.35
>10	3.801765105227427	26.575
>50	0.20366598778004072	4.05
>100	0.20366598778004072	16.55
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	267	6.675000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	230	5.75	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	165	4.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC	56	1.4000000000000001	RNA PCR Primer, Index 10 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	52	1.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	32	0.8	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	32	0.8	RNA PCR Primer, Index 7 (97% over 35bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	23	0.575	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21	0.525	RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG	16	0.4	RNA PCR Primer, Index 7 (97% over 36bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	15	0.375	No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC	11	0.27499999999999997	RNA PCR Primer, Index 10 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	11	0.27499999999999997	No Hit
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	11	0.27499999999999997	No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTCTAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	10	0.25	RNA PCR Primer, Index 7 (97% over 35bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACTAGCTTATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 10 (98% over 50bp)
TGAGGTAGTAGGTTGTCTAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AAGCTGCCAGTTGAAGACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT	6	0.15	RNA PCR Primer, Index 10 (100% over 38bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACCCTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG	6	0.15	RNA PCR Primer, Index 10 (100% over 49bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCCGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT	6	0.15	RNA PCR Primer, Index 10 (100% over 39bp)
TGTAAACATCCCCGACTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC	5	0.125	RNA PCR Primer, Index 10 (100% over 37bp)
TAGCAGCACAGAAATATTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	5	0.125	RNA PCR Primer, Index 3 (100% over 34bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
TGAGGTAGTAGTTTGTCCAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG	5	0.125	RNA PCR Primer, Index 10 (100% over 45bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGCTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTCTGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.85	0.0	0.0	0.0
2	0.0	2.025	0.0	0.0	0.0
3	0.0	2.175	0.0	0.0	0.0
4	0.0	2.225	0.0	0.0	0.0
5	0.0	2.35	0.0	0.0	0.0
6	0.0	2.575	0.0	0.0	0.0
7	0.0	3.1	0.0	0.0	0.0
8	0.0	3.375	0.0	0.0	0.0
9	0.0	3.5	0.0	0.0	0.0
10	0.0	3.725	0.0	0.0	0.0
11	0.0	3.975	0.0	0.0	0.0
12	0.0	4.4	0.0	0.0	0.0
13	0.0	4.775	0.0	0.0	0.0
14	0.0	5.2	0.0	0.0	0.0
15	0.0	5.925	0.0	0.0	0.0
16	0.0	7.125	0.0	0.0	0.0
17	0.0	7.8	0.0	0.0	0.0
18	0.0	8.925	0.0	0.0	0.0
19	0.0	9.575	0.0	0.0	0.0
20	0.0	10.925	0.0	0.0	0.0
21	0.0	14.7	0.0	0.0	0.0
22	0.0	26.55	0.0	0.0	0.0
23	0.0	62.25	0.0	0.0	0.0
24	0.0	79.125	0.0	0.0	0.0
25	0.0	85.0	0.0	0.0	0.0
26	0.0	86.0	0.0	0.0	0.0
27	0.0	86.45	0.0	0.0	0.0
28	0.0	87.025	0.0	0.0	0.0
29	0.0	87.35	0.0	0.0	0.0
30	0.0	87.775	0.0	0.0	0.0
31	0.0	88.325	0.0	0.0	0.0
32	0.0	89.05	0.0	0.0	0.0
33	0.0	90.025	0.0	0.0	0.0
34	0.0	91.325	0.0	0.0	0.0
35	0.0	91.9	0.0	0.0	0.0
36	0.0	92.175	0.0	0.0	0.0
37	0.0	92.575	0.0	0.0	0.0
38	0.0	92.775	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCCG	20	6.952051E-4	44.000004	8
GAGGTAG	20	6.952051E-4	44.000004	2
AAGTAAT	40	5.315087E-9	44.000004	4
TCAAGTA	40	5.315087E-9	44.000004	2
AATCCAG	40	5.315087E-9	44.000004	8
ATCCAGG	40	5.315087E-9	44.000004	9
GAGTGGA	20	6.952051E-4	44.000004	20
TTCAAGT	40	5.315087E-9	44.000004	1
CATCCCC	20	6.952051E-4	44.000004	7
AGTGGAA	20	6.952051E-4	44.000004	21
TCCAGGA	40	5.315087E-9	44.000004	10
GTGAGTG	20	6.952051E-4	44.000004	18
ACATCCC	20	6.952051E-4	44.000004	6
ACATTCA	65	0.0	44.000004	2
CCCCGAC	20	6.952051E-4	44.000004	10
AGTAATC	40	5.315087E-9	44.000004	5
AGGCTTG	20	6.952051E-4	44.000004	18
CCCGACT	20	6.952051E-4	44.000004	11
TAATCCA	40	5.315087E-9	44.000004	7
GGTAGTA	20	6.952051E-4	44.000004	4
>>END_MODULE
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
Read 317069 spots for SRR5031086.sra
Written 317069 spots for SRR5031086.sra
Read 317059 spots for SRR5031086.sra
Written 317059 spots for SRR5031086.sra
SRR ids: ['SRR5031086.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x3aifn7h
SRR5031086.sra spots: 3804718
blocks: [[1, 317059], [317060, 634118], [634119, 951177], [951178, 1268236], [1268237, 1585295], [1585296, 1902354], [1902355, 2219413], [2219414, 2536472], [2536473, 2853531], [2853532, 3170590], [3170591, 3487649], [3487650, 3804718]]
SRR5031086 file size 658388
SRR5031086 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031086 SRR5031086_1.fastq
Input file:	SRR5031086_1.fastq
trimmed:	SRR5031086-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:21:33 2020 >> started

Wed Mar  4 07:21:37 2020 >> done (3.694s)
3804718 reads processed; of these:
    193 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
3804519 (99.99%) reads available; of these:
 284685 ( 7.48%) trimmed reads available after processing
3519834 (92.52%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     66	  0.00%
 19	    108	  0.00%
 20	    216	  0.01%
 21	    323	  0.01%
 22	    575	  0.02%
 23	    627	  0.02%
 24	    637	  0.02%
 25	    898	  0.02%
 26	    647	  0.02%
 27	    820	  0.02%
 28	    993	  0.03%
 29	   2088	  0.05%
 30	    970	  0.03%
 31	   1084	  0.03%
 32	   1323	  0.03%
 33	   2676	  0.07%
 34	   3864	  0.10%
 35	  11479	  0.30%
 36	   6404	  0.17%
 37	   5479	  0.14%
 38	   4663	  0.12%
 39	   5395	  0.14%
 40	   6532	  0.17%
 41	   6777	  0.18%
 42	   8809	  0.23%
 43	  14088	  0.37%
 44	  14596	  0.38%
 45	  21232	  0.56%
 46	  22003	  0.58%
 47	  21179	  0.56%
 48	  59027	  1.55%
 49	  59107	  1.55%
 50	3519834	 92.52%
3804519 reads passed initial QC


criterion=sequence-density
sequence-density=93.42
sequence-density-rank=1
fanout-score=16.07
fanout-score-rank=2
prefix-density=92.54
prefix-fanout=16.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC


criterion=fanout-score
sequence-density=3.64
sequence-density-rank=4
fanout-score=71.71
fanout-score-rank=1
prefix-density=93.14
prefix-fanout=2.8
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC -o SRR5031086 -
Input file:	STDIN
trimmed:	SRR5031086-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:22:04 2020 >> started

Wed Mar  4 07:22:14 2020 >> done (9.424s)
3723572 reads processed; of these:
 294159 ( 7.90%) short reads filtered out after trimming by size control
  63425 ( 1.70%) empty reads filtered out after trimming by size control
3365988 (90.40%) reads available; of these:
3297176 (97.96%) trimmed reads available after processing
  68812 ( 2.04%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  22492	  0.67%
 19	  53412	  1.59%
 20	 193948	  5.76%
 21	 446438	 13.26%
 22	1278084	 37.97%
 23	 671625	 19.95%
 24	 242163	  7.19%
 25	  35433	  1.05%
 26	  18876	  0.56%
 27	  17937	  0.53%
 28	  18237	  0.54%
 29	  13921	  0.41%
 30	  23638	  0.70%
 31	  32807	  0.97%
 32	  31640	  0.94%
 33	  59424	  1.77%
 34	  24507	  0.73%
 35	  13647	  0.41%
 36	  13645	  0.41%
 37	   9041	  0.27%
 38	   8321	  0.25%
 39	   7697	  0.23%
 40	   8457	  0.25%
 41	  11509	  0.34%
 42	  11814	  0.35%
 43	   9419	  0.28%
 44	   8216	  0.24%
 45	   9906	  0.29%
 46	   7886	  0.23%
 47	   5591	  0.17%
 48	    795	  0.02%
 49	    973	  0.03%
 50	  54489	  1.62%


criterion=sequence-density
sequence-density=14.05
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=18
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.43
sequence-density-rank=32
fanout-score=27.39
fanout-score-rank=1
prefix-density=11.69
prefix-fanout=1.0
sequence=AGTAATCCAGGCTAGGCTT
                                 Started job on |	Mar 04 07:22:42
                             Started mapping on |	Mar 04 07:22:43
                                    Finished on |	Mar 04 07:23:09
       Mapping speed, Million of reads per hour |	477.27

                          Number of input reads |	3446935
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1496828
                        Uniquely mapped reads % |	43.42%
                          Average mapped length |	23.03
                       Number of splices: Total |	4800
            Number of splices: Annotated (sjdb) |	830
                       Number of splices: GT/AG |	4687
                       Number of splices: GC/AG |	44
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	68
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.14
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1664931
             % of reads mapped to multiple loci |	48.30%
        Number of reads mapped to too many loci |	64340
             % of reads mapped to too many loci |	1.87%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.68%
                     % of reads unmapped: other |	0.73%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	285176	285176	285176
N_multimapping	1664931	1664931	1664931
N_noFeature	86487	97121	1136404
N_ambiguous	762299	445016	6925
UnstrandedReadsAssigned:648042 PositiveStrandReadsAssigned:954691 NegativeStrandReadsAssigned:353499
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031086 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031086-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,446,935 reads, 534,490 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,096 rounds

 109283 SRR5031086.ke.tsv
  52636 SRR5031086.se.tsv
 161919 total
==> SRR5031086.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031086.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031086 completed mapping pipeline successfully
