Starting /dee2/code/volunteer_pipeline.sh SRR5031065
    current disk space = 160511279104
    free memory = 73927892 
SRR5031065 SRAfilesize
0a5ad01ad29c3e3c008b87499dbd1e89  SRR5031065.sra
SRR5031065.sra file validated
SRR5031065 is single end
SRR5031065 is conventional basespace
SRR5031065 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3305	33.0	31.0	34.0	30.0	34.0
2	32.60375	34.0	31.0	34.0	31.0	34.0
3	32.63125	34.0	31.0	34.0	31.0	34.0
4	36.21325	37.0	35.0	37.0	35.0	37.0
5	35.97275	37.0	35.0	37.0	35.0	37.0
6	35.93025	37.0	35.0	37.0	35.0	37.0
7	35.96375	37.0	35.0	37.0	35.0	37.0
8	35.84425	37.0	35.0	37.0	35.0	37.0
9	37.62325	39.0	37.0	39.0	35.0	39.0
10	37.798	39.0	38.0	39.0	35.0	39.0
11	37.609	39.0	37.0	39.0	35.0	39.0
12	37.59975	39.0	37.0	39.0	35.0	39.0
13	37.58525	39.0	37.0	39.0	35.0	39.0
14	38.98325	41.0	38.0	41.0	35.0	41.0
15	38.886	40.0	38.0	41.0	35.0	41.0
16	38.93875	40.0	38.0	41.0	35.0	41.0
17	38.767	40.0	38.0	41.0	34.0	41.0
18	38.929	40.0	38.0	41.0	35.0	41.0
19	38.669	40.0	38.0	41.0	34.0	41.0
20	38.69775	40.0	38.0	41.0	34.0	41.0
21	38.4485	40.0	38.0	41.0	34.0	41.0
22	38.403	40.0	38.0	41.0	34.0	41.0
23	38.11475	40.0	37.0	41.0	34.0	41.0
24	38.213	40.0	38.0	41.0	34.0	41.0
25	38.63325	40.0	38.0	41.0	34.0	41.0
26	38.491	40.0	38.0	41.0	34.0	41.0
27	38.623	40.0	38.0	41.0	34.0	41.0
28	38.702	40.0	38.0	41.0	35.0	41.0
29	38.17375	40.0	38.0	41.0	33.0	41.0
30	38.0165	40.0	38.0	41.0	32.0	41.0
31	38.28825	40.0	38.0	41.0	33.0	41.0
32	38.31175	40.0	38.0	41.0	33.0	41.0
33	38.03225	40.0	38.0	41.0	33.0	41.0
34	38.179	40.0	38.0	41.0	33.0	41.0
35	38.255	40.0	38.0	41.0	34.0	41.0
36	38.07075	40.0	38.0	41.0	33.0	41.0
37	37.502	40.0	37.0	41.0	31.0	41.0
38	36.82075	39.0	36.0	41.0	30.0	41.0
39	37.21275	39.0	37.0	41.0	31.0	41.0
40	37.351	40.0	37.0	41.0	32.0	41.0
41	37.48925	40.0	37.0	41.0	32.0	41.0
42	37.811	40.0	37.0	41.0	33.0	41.0
43	38.0775	40.0	38.0	41.0	33.0	41.0
44	38.01075	40.0	38.0	41.0	33.0	41.0
45	37.84425	40.0	38.0	41.0	33.0	41.0
46	37.62725	40.0	37.0	41.0	33.0	41.0
47	37.027	40.0	36.0	41.0	31.0	41.0
48	36.3985	39.0	35.0	41.0	30.0	41.0
49	36.6725	39.0	36.0	41.0	31.0	41.0
50	36.2695	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	4.0
16	2.0
17	0.0
18	2.0
19	4.0
20	3.0
21	5.0
22	7.0
23	12.0
24	7.0
25	13.0
26	17.0
27	14.0
28	33.0
29	37.0
30	53.0
31	61.0
32	89.0
33	100.0
34	150.0
35	190.0
36	273.0
37	429.0
38	755.0
39	1738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.375	24.099999999999998	47.949999999999996	16.575
2	24.887330996494743	26.589884827240862	20.70605908863295	27.81672508763145
3	23.275000000000002	17.65	21.95	37.125
4	35.35	28.775000000000002	14.075	21.8
5	22.2	36.55	26.674999999999997	14.575
6	29.075	16.875	37.724999999999994	16.325
7	18.625	20.0	21.15	40.225
8	21.95	27.975	20.45	29.625
9	24.275	33.0	24.725	18.0
10	23.525	19.900000000000002	29.549999999999997	27.025
11	33.125	11.725	17.549999999999997	37.6
12	15.225	22.3	31.2	31.275
13	27.55	16.8	33.925	21.725
14	30.175	30.15	20.625	19.05
15	37.574999999999996	22.400000000000002	25.674999999999997	14.35
16	20.025000000000002	19.125	24.224999999999998	36.625
17	27.3	24.224999999999998	34.1	14.374999999999998
18	27.55	17.25	30.85	24.349999999999998
19	36.175000000000004	14.649999999999999	32.324999999999996	16.85
20	43.65	16.35	26.0	14.000000000000002
21	25.374999999999996	21.925	23.175	29.525000000000002
22	30.2	13.725000000000001	45.625	10.45
23	24.0	14.274999999999999	43.625	18.099999999999998
24	50.449999999999996	15.1	26.075	8.375
25	48.8	23.225	16.2	11.774999999999999
26	27.85	44.4	13.525	14.224999999999998
27	16.1	45.75	23.25	14.899999999999999
28	12.375	23.674999999999997	46.85	17.1
29	14.774999999999999	13.475000000000001	53.849999999999994	17.9
30	10.95	6.950000000000001	35.05	47.05
31	14.149999999999999	5.75	47.275	32.824999999999996
32	26.525	6.425	21.875	45.175
33	51.675000000000004	10.4	15.15	22.775000000000002
34	58.550000000000004	8.924999999999999	16.1	16.425
35	65.225	7.55	17.299999999999997	9.925
36	41.25	8.625	37.25	12.875
37	44.800000000000004	13.225000000000001	20.0	21.975
38	23.525	17.75	13.25	45.475
39	16.2	22.6	12.049999999999999	49.15
40	15.125	46.475	12.725	25.674999999999997
41	23.925	46.35	10.274999999999999	19.45
42	48.225	27.375	12.9	11.5
43	50.775000000000006	27.950000000000003	9.275	12.0
44	32.975	46.5	7.6	12.925
45	21.325	47.85	13.25	17.575
46	15.625	25.25	17.325	41.8
47	11.025	15.950000000000001	39.5	33.525
48	12.525	12.75	19.950000000000003	54.775
49	13.55	18.05	14.374999999999998	54.025
50	19.55	36.325	12.1	32.025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	1.0
22	1.0
23	1.0
24	1.0
25	0.5
26	0.0
27	0.0
28	0.0
29	2.0
30	4.0
31	6.5
32	9.0
33	13.5
34	18.0
35	18.0
36	18.0
37	23.5
38	29.0
39	31.5
40	34.0
41	42.0
42	50.0
43	82.0
44	114.0
45	142.5
46	171.0
47	289.5
48	408.0
49	519.5
50	631.0
51	734.5
52	838.0
53	633.0
54	428.0
55	460.0
56	492.0
57	391.0
58	290.0
59	197.5
60	105.0
61	82.5
62	60.0
63	106.0
64	152.0
65	91.5
66	31.0
67	25.0
68	19.0
69	16.5
70	14.0
71	38.0
72	62.0
73	33.5
74	5.0
75	3.5
76	2.0
77	3.5
78	5.0
79	3.0
80	1.0
81	2.5
82	4.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.050000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05633802816901	29.7
2	9.923175416133162	7.75
3	3.841229193341869	4.5
4	2.112676056338028	3.3000000000000003
5	1.6645326504481435	3.25
6	1.088348271446863	2.55
7	0.8962868117797695	2.45
8	0.6402048655569782	2.0
9	0.3201024327784891	1.125
>10	2.880921895006402	20.325
>50	0.19206145966709345	5.325
>100	0.3841229193341869	17.724999999999998
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	124	3.1	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	120	3.0	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	111	2.775	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	105	2.625	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	85	2.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	76	1.9	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	52	1.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	50	1.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	36	0.8999999999999999	RNA PCR Primer, Index 2 (100% over 35bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	26	0.65	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGC	23	0.575	RNA PCR Primer, Index 37 (100% over 50bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTC	22	0.5499999999999999	RNA PCR Primer, Index 37 (100% over 44bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	12	0.3	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	11	0.27499999999999997	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	9	0.22499999999999998	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CGCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGG	7	0.17500000000000002	Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	6	0.15	RNA PCR Primer, Index 2 (100% over 35bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	6	0.15	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG	6	0.15	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTAT	5	0.125	RNA PCR Primer, Index 37 (100% over 48bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
CTAGACTGAGGCTCCTTGAGGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC	5	0.125	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTC	5	0.125	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGG	5	0.125	RNA PCR Primer, Index 7 (97% over 36bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.6	0.0	0.0	0.0
2	0.0	0.625	0.0	0.0	0.0
3	0.0	0.75	0.0	0.0	0.0
4	0.0	0.8	0.0	0.0	0.0
5	0.0	0.85	0.0	0.0	0.0
6	0.0	0.975	0.0	0.0	0.0
7	0.0	1.625	0.0	0.0	0.0
8	0.0	1.8	0.0	0.0	0.0
9	0.0	1.95	0.0	0.0	0.0
10	0.0	2.1	0.0	0.0	0.0
11	0.0	2.225	0.0	0.0	0.0
12	0.0	2.525	0.0	0.0	0.0
13	0.0	2.65	0.0	0.0	0.0
14	0.0	2.825	0.0	0.0	0.0
15	0.0	3.25	0.0	0.0	0.0
16	0.0	4.775	0.0	0.0	0.0
17	0.0	5.55	0.0	0.0	0.0
18	0.0	6.575	0.0	0.0	0.0
19	0.0	7.15	0.0	0.0	0.0
20	0.0	7.95	0.0	0.0	0.0
21	0.0	12.55	0.0	0.0	0.0
22	0.0	22.325	0.0	0.0	0.0
23	0.0	51.325	0.0	0.0	0.0
24	0.0	63.35	0.0	0.0	0.0
25	0.0	69.475	0.0	0.0	0.0
26	0.0	70.85	0.0	0.0	0.0
27	0.0	71.7	0.0	0.0	0.0
28	0.0	72.25	0.0	0.0	0.0
29	0.0	72.925	0.0	0.0	0.0
30	0.0	73.75	0.0	0.0	0.0
31	0.0	74.75	0.0	0.0	0.0
32	0.0	76.075	0.0	0.0	0.0
33	0.0	77.675	0.0	0.0	0.0
34	0.0	82.125	0.0	0.0	0.0
35	0.0	83.425	0.0	0.0	0.0
36	0.0	84.625	0.0	0.0	0.0
37	0.0	85.925	0.0	0.0	0.0
38	0.0	86.425	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCATTG	20	6.952051E-4	44.000004	5
AATCCAG	20	6.952051E-4	44.000004	8
CTGAGCT	40	5.315087E-9	44.000004	11
ATCCAGG	20	6.952051E-4	44.000004	9
TCGGTGG	20	6.952051E-4	44.000004	15
ATAGGCT	20	6.952051E-4	44.000004	16
GAGCTTG	40	5.315087E-9	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
ACCCGTA	20	6.952051E-4	44.000004	2
CCGTCTG	40	5.315087E-9	44.000004	7
CATTCAT	20	6.952051E-4	44.000004	3
TCCAGGA	20	6.952051E-4	44.000004	10
CTTGGCT	40	5.315087E-9	44.000004	16
CCGTAGA	20	6.952051E-4	44.000004	4
CCCGTAG	20	6.952051E-4	44.000004	3
GTGGGTG	20	6.952051E-4	44.000004	18
AGTAATC	20	6.952051E-4	44.000004	5
GATCCGT	40	5.315087E-9	44.000004	4
CGGTGGG	20	6.952051E-4	44.000004	16
TAATCCA	20	6.952051E-4	44.000004	7
>>END_MODULE
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206191 spots for SRR5031065.sra
Written 206191 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
Read 206180 spots for SRR5031065.sra
Written 206180 spots for SRR5031065.sra
SRR ids: ['SRR5031065.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fyupl0sv
SRR5031065.sra spots: 2474171
blocks: [[1, 206180], [206181, 412360], [412361, 618540], [618541, 824720], [824721, 1030900], [1030901, 1237080], [1237081, 1443260], [1443261, 1649440], [1649441, 1855620], [1855621, 2061800], [2061801, 2267980], [2267981, 2474171]]
SRR5031065 file size 427762
SRR5031065 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031065 SRR5031065_1.fastq
Input file:	SRR5031065_1.fastq
trimmed:	SRR5031065-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:02 2020 >> started

Wed Mar  4 07:17:04 2020 >> done (1.974s)
2474171 reads processed; of these:
    210 ( 0.01%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
2473953 (99.99%) reads available; of these:
 130403 ( 5.27%) trimmed reads available after processing
2343550 (94.73%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     57	  0.00%
 19	     77	  0.00%
 20	    169	  0.01%
 21	    227	  0.01%
 22	    389	  0.02%
 23	    384	  0.02%
 24	    373	  0.02%
 25	    596	  0.02%
 26	    452	  0.02%
 27	    593	  0.02%
 28	    653	  0.03%
 29	   1023	  0.04%
 30	    671	  0.03%
 31	    844	  0.03%
 32	    941	  0.04%
 33	   1098	  0.04%
 34	   1167	  0.05%
 35	   2007	  0.08%
 36	   1895	  0.08%
 37	   2124	  0.09%
 38	   2203	  0.09%
 39	   2433	  0.10%
 40	   2737	  0.11%
 41	   2774	  0.11%
 42	   3514	  0.14%
 43	   4766	  0.19%
 44	   7370	  0.30%
 45	   9724	  0.39%
 46	  13679	  0.55%
 47	  17012	  0.69%
 48	  24203	  0.98%
 49	  24248	  0.98%
 50	2343550	 94.73%
2473953 reads passed initial QC


criterion=sequence-density
sequence-density=85.65
sequence-density-rank=1
fanout-score=21.76
fanout-score-rank=1
prefix-density=88.04
prefix-fanout=21.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC


criterion=fanout-score
sequence-density=85.65
sequence-density-rank=1
fanout-score=21.76
fanout-score-rank=1
prefix-density=88.04
prefix-fanout=21.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC -o SRR5031065 -
Input file:	STDIN
trimmed:	SRR5031065-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGGAATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:17:18 2020 >> started

Wed Mar  4 07:17:21 2020 >> done (3.144s)
2416419 reads processed; of these:
 125509 ( 5.19%) short reads filtered out after trimming by size control
  12437 ( 0.51%) empty reads filtered out after trimming by size control
2278473 (94.29%) reads available; of these:
2171609 (95.31%) trimmed reads available after processing
 106864 ( 4.69%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  12075	  0.53%
 19	  21397	  0.94%
 20	 126117	  5.54%
 21	 255470	 11.21%
 22	 692500	 30.39%
 23	 310601	 13.63%
 24	 144685	  6.35%
 25	  42282	  1.86%
 26	  22343	  0.98%
 27	  18393	  0.81%
 28	  20443	  0.90%
 29	  16896	  0.74%
 30	  22971	  1.01%
 31	  31987	  1.40%
 32	  32020	  1.41%
 33	  99603	  4.37%
 34	  36068	  1.58%
 35	  30001	  1.32%
 36	  35066	  1.54%
 37	  15501	  0.68%
 38	  17949	  0.79%
 39	  16045	  0.70%
 40	  24019	  1.05%
 41	  28639	  1.26%
 42	  31812	  1.40%
 43	  21563	  0.95%
 44	  16084	  0.71%
 45	  20415	  0.90%
 46	  14703	  0.65%
 47	  11826	  0.52%
 48	   1485	  0.07%
 49	   1640	  0.07%
 50	  85874	  3.77%


criterion=sequence-density
sequence-density=7.66
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.76
sequence-density-rank=22
fanout-score=11.06
fanout-score-rank=1
prefix-density=8.43
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 07:17:42
                             Started mapping on |	Mar 04 07:17:42
                                    Finished on |	Mar 04 07:18:06
       Mapping speed, Million of reads per hour |	350.40

                          Number of input reads |	2336007
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1249700
                        Uniquely mapped reads % |	53.50%
                          Average mapped length |	24.81
                       Number of splices: Total |	1574
            Number of splices: Annotated (sjdb) |	269
                       Number of splices: GT/AG |	1470
                       Number of splices: GC/AG |	23
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	81
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.73
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	756540
             % of reads mapped to multiple loci |	32.39%
        Number of reads mapped to too many loci |	55387
             % of reads mapped to too many loci |	2.37%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.84%
                     % of reads unmapped: other |	0.90%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	329767	329767	329767
N_multimapping	756540	756540	756540
N_noFeature	63191	82053	830714
N_ambiguous	684992	318003	10916
UnstrandedReadsAssigned:501517 PositiveStrandReadsAssigned:849644 NegativeStrandReadsAssigned:408070
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031065 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031065-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,336,007 reads, 590,666 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,159 rounds

 109283 SRR5031065.ke.tsv
  52636 SRR5031065.se.tsv
 161919 total
==> SRR5031065.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031065.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031065 completed mapping pipeline successfully
