Starting /dee2/code/volunteer_pipeline.sh SRR5031005
    current disk space = 160554237952
    free memory = 73938768 
SRR5031005 SRAfilesize
a5949e6162f9f3bbccdf57e0716f7a57  SRR5031005.sra
SRR5031005.sra file validated
SRR5031005 is single end
SRR5031005 is conventional basespace
SRR5031005 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3605	33.0	31.0	34.0	31.0	34.0
2	32.554	34.0	31.0	34.0	31.0	34.0
3	32.5085	34.0	31.0	34.0	31.0	34.0
4	36.17875	37.0	35.0	37.0	35.0	37.0
5	36.1295	37.0	35.0	37.0	35.0	37.0
6	35.894	37.0	35.0	37.0	33.0	37.0
7	35.95325	37.0	35.0	37.0	35.0	37.0
8	36.02	37.0	35.0	37.0	35.0	37.0
9	37.62925	39.0	37.0	39.0	35.0	39.0
10	37.56475	39.0	37.0	39.0	35.0	39.0
11	37.25225	39.0	37.0	39.0	34.0	39.0
12	37.63125	39.0	37.0	39.0	35.0	39.0
13	37.591	39.0	37.0	39.0	35.0	39.0
14	39.17875	40.0	39.0	41.0	36.0	41.0
15	39.045	40.0	38.0	41.0	35.0	41.0
16	38.99025	40.0	38.0	41.0	36.0	41.0
17	39.14825	40.0	38.0	41.0	36.0	41.0
18	39.0085	41.0	38.0	41.0	35.0	41.0
19	38.85925	40.0	38.0	41.0	35.0	41.0
20	38.79075	40.0	38.0	41.0	35.0	41.0
21	38.93075	40.0	38.0	41.0	35.0	41.0
22	38.98275	40.0	39.0	41.0	35.0	41.0
23	38.651	40.0	38.0	41.0	34.0	41.0
24	38.9595	40.0	38.0	41.0	35.0	41.0
25	39.11625	41.0	39.0	41.0	35.0	41.0
26	38.81225	40.0	38.0	41.0	35.0	41.0
27	38.942	40.0	39.0	41.0	35.0	41.0
28	38.85775	40.0	38.0	41.0	35.0	41.0
29	38.58675	40.0	38.0	41.0	34.0	41.0
30	38.68325	40.0	38.0	41.0	34.0	41.0
31	38.897	40.0	38.0	41.0	35.0	41.0
32	38.956	40.0	38.0	41.0	35.0	41.0
33	38.81625	40.0	39.0	41.0	35.0	41.0
34	38.5735	40.0	38.0	41.0	34.0	41.0
35	38.265	40.0	38.0	41.0	34.0	41.0
36	38.0165	40.0	37.0	41.0	33.0	41.0
37	37.50875	40.0	37.0	41.0	31.0	41.0
38	37.4885	39.0	37.0	41.0	31.0	41.0
39	37.94525	40.0	37.0	41.0	33.0	41.0
40	37.98025	40.0	37.0	41.0	33.0	41.0
41	38.247	40.0	38.0	41.0	34.0	41.0
42	38.24575	40.0	38.0	41.0	34.0	41.0
43	37.9855	40.0	37.0	41.0	33.0	41.0
44	38.08175	40.0	38.0	41.0	33.0	41.0
45	37.95175	40.0	38.0	41.0	33.0	41.0
46	37.86575	40.0	37.0	41.0	33.0	41.0
47	37.54975	40.0	37.0	41.0	32.0	41.0
48	37.23025	40.0	37.0	41.0	31.0	41.0
49	37.22775	40.0	37.0	41.0	31.0	41.0
50	36.58725	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	1.0
17	1.0
18	0.0
19	3.0
20	4.0
21	3.0
22	5.0
23	5.0
24	5.0
25	11.0
26	9.0
27	10.0
28	17.0
29	34.0
30	43.0
31	52.0
32	85.0
33	107.0
34	129.0
35	209.0
36	245.0
37	454.0
38	713.0
39	1853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.775	22.225	56.699999999999996	13.3
2	25.88501129801657	27.29098669344715	21.26537785588752	25.558624152648758
3	17.150000000000002	19.7	23.125	40.025
4	26.900000000000002	37.5	13.8	21.8
5	21.5	37.85	28.725	11.924999999999999
6	34.849999999999994	16.7	35.85	12.6
7	14.374999999999998	25.025	23.200000000000003	37.4
8	23.65	41.65	15.075	19.625
9	18.3	36.6	31.624999999999996	13.475000000000001
10	19.325	21.525	24.6	34.55
11	30.85	9.875	16.1	43.175000000000004
12	17.25	16.6	24.375	41.775
13	19.875	20.1	38.7	21.325
14	39.25	23.125	24.325	13.3
15	39.074999999999996	22.525000000000002	27.474999999999998	10.925
16	12.4	23.474999999999998	24.175	39.95
17	25.775	22.075	39.175	12.975
18	32.300000000000004	17.549999999999997	27.825	22.325
19	39.050000000000004	13.325000000000001	31.125000000000004	16.5
20	47.925000000000004	19.55	20.674999999999997	11.85
21	20.424999999999997	28.1	24.875	26.6
22	32.625	12.925	46.800000000000004	7.6499999999999995
23	24.675	13.950000000000001	47.775	13.600000000000001
24	51.37500000000001	13.0	29.875	5.75
25	54.275	22.3	15.049999999999999	8.375
26	30.275000000000002	47.85	12.275	9.6
27	13.850000000000001	51.975	23.425	10.75
28	9.0	27.025	50.949999999999996	13.025
29	9.9	11.799999999999999	59.325	18.975
30	9.9	4.725	40.050000000000004	45.324999999999996
31	12.675	3.95	45.95	37.425000000000004
32	25.4	4.775	24.55	45.275
33	55.25	5.775	13.65	25.324999999999996
34	67.30000000000001	6.7	13.025	12.975
35	67.30000000000001	6.800000000000001	17.5	8.4
36	43.5	7.225	38.35	10.925
37	45.675	8.975	23.175	22.175
38	25.15	13.825000000000001	12.4	48.625
39	14.674999999999999	22.575	8.525	54.225
40	12.725	50.324999999999996	9.0	27.950000000000003
41	23.150000000000002	53.55	8.1	15.2
42	50.224999999999994	31.525	9.475	8.774999999999999
43	56.974999999999994	27.55	7.074999999999999	8.4
44	32.75	49.525000000000006	6.225	11.5
45	16.375	53.949999999999996	11.575000000000001	18.099999999999998
46	10.775	28.525	17.75	42.95
47	7.95	13.925	39.15	38.975
48	8.325000000000001	10.674999999999999	23.474999999999998	57.525000000000006
49	11.0	19.775000000000002	12.975	56.25
50	17.75	38.800000000000004	10.7	32.75
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	0.5
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	1.0
30	2.0
31	10.0
32	18.0
33	14.0
34	10.0
35	17.5
36	25.0
37	27.5
38	30.0
39	31.5
40	33.0
41	36.0
42	39.0
43	77.0
44	115.0
45	178.5
46	242.0
47	433.5
48	625.0
49	654.0
50	683.0
51	838.0
52	993.0
53	658.5
54	324.0
55	332.5
56	341.0
57	299.0
58	257.0
59	150.0
60	43.0
61	41.5
62	40.0
63	51.0
64	62.0
65	39.5
66	17.0
67	13.0
68	9.0
69	11.5
70	14.0
71	41.0
72	68.0
73	35.5
74	3.0
75	2.0
76	1.0
77	1.0
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.42500000000000004
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66383581033263	26.375
2	9.200283085633403	6.5
3	5.024769992922859	5.325
4	1.48619957537155	2.1
5	1.910828025477707	3.375
6	1.556970983722576	3.3000000000000003
7	0.4953998584571833	1.225
8	0.6369426751592357	1.7999999999999998
9	0.4953998584571833	1.575
>10	3.9631988676574665	26.375
>50	0.28308563340410475	5.675
>100	0.28308563340410475	16.375
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	235	5.875	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	162	4.05	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	147	3.675	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	111	2.775	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	65	1.625	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	55	1.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	55	1.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	42	1.05	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	39	0.975	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	34	0.8500000000000001	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	31	0.775	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	30	0.75	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	27	0.675	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC	25	0.625	RNA PCR Primer, Index 5 (100% over 35bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21	0.525	RNA PCR Primer, Index 1 (100% over 33bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC	19	0.475	RNA PCR Primer, Index 25 (100% over 50bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	10	0.25	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	9	0.22499999999999998	No Hit
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	9	0.22499999999999998	No Hit
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	8	0.2	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	8	0.2	No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	8	0.2	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC	6	0.15	RNA PCR Primer, Index 25 (100% over 44bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	6	0.15	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	6	0.15	No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	6	0.15	No Hit
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGCTACACATTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	5	0.125	RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCAGGCTCAGTCCCCTCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.525	0.0	0.0	0.0
2	0.0	0.55	0.0	0.0	0.0
3	0.0	0.6	0.0	0.0	0.0
4	0.0	0.625	0.0	0.0	0.0
5	0.0	0.65	0.0	0.0	0.0
6	0.0	0.7	0.0	0.0	0.0
7	0.0	0.975	0.0	0.0	0.0
8	0.0	1.075	0.0	0.0	0.0
9	0.0	1.1	0.0	0.0	0.0
10	0.0	1.175	0.0	0.0	0.0
11	0.0	1.25	0.0	0.0	0.0
12	0.0	1.35	0.0	0.0	0.0
13	0.0	1.6	0.0	0.0	0.0
14	0.0	1.75	0.0	0.0	0.0
15	0.0	1.975	0.0	0.0	0.0
16	0.0	3.0	0.0	0.0	0.0
17	0.0	3.625	0.0	0.0	0.0
18	0.0	4.7	0.0	0.0	0.0
19	0.0	5.2	0.0	0.0	0.0
20	0.0	6.3	0.0	0.0	0.0
21	0.0	10.9	0.0	0.0	0.0
22	0.0	22.8	0.0	0.0	0.0
23	0.0	54.475	0.0	0.0	0.0
24	0.0	71.025	0.0	0.0	0.0
25	0.0	77.2	0.0	0.0	0.0
26	0.0	78.375	0.0	0.0	0.0
27	0.0	78.9	0.0	0.0	0.0
28	0.0	79.5	0.0	0.0	0.0
29	0.0	80.075	0.0	0.0	0.0
30	0.0	80.575	0.0	0.0	0.0
31	0.0	81.325	0.0	0.0	0.0
32	0.0	82.725	0.0	0.0	0.0
33	0.0	83.9	0.0	0.0	0.0
34	0.0	85.9	0.0	0.0	0.0
35	0.0	86.9	0.0	0.0	0.0
36	0.0	87.55	0.0	0.0	0.0
37	0.0	88.375	0.0	0.0	0.0
38	0.0	88.65	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCG	20	6.952051E-4	44.000004	8
AACGCTG	40	5.315087E-9	44.000004	8
GAGGTAG	40	5.315087E-9	44.000004	2
TCCTCGA	20	6.952051E-4	44.000004	9
AAGTAAT	45	2.728484E-10	44.000004	4
GGTGAAT	20	6.952051E-4	44.000004	11
TCAAGTA	45	2.728484E-10	44.000004	2
ACGCTGT	40	5.315087E-9	44.000004	9
AATCCAG	45	2.728484E-10	44.000004	8
GGCACGC	20	6.952051E-4	44.000004	4
TCGACTG	20	6.952051E-4	44.000004	12
ATCCAGG	45	2.728484E-10	44.000004	9
AGTAGGT	20	6.952051E-4	44.000004	7
TTCAAGT	45	2.728484E-10	44.000004	1
TTCAACG	40	5.315087E-9	44.000004	5
AGGATAG	45	2.728484E-10	44.000004	13
CACGCGG	20	6.952051E-4	44.000004	6
CATCCTC	20	6.952051E-4	44.000004	7
ATAGGCT	45	2.728484E-10	44.000004	16
GCGGTGA	20	6.952051E-4	44.000004	9
>>END_MODULE
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373575 spots for SRR5031005.sra
Written 373575 spots for SRR5031005.sra
Read 373581 spots for SRR5031005.sra
Written 373581 spots for SRR5031005.sra
SRR ids: ['SRR5031005.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_oek2vda7
SRR5031005.sra spots: 4482906
blocks: [[1, 373575], [373576, 747150], [747151, 1120725], [1120726, 1494300], [1494301, 1867875], [1867876, 2241450], [2241451, 2615025], [2615026, 2988600], [2988601, 3362175], [3362176, 3735750], [3735751, 4109325], [4109326, 4482906]]
SRR5031005 file size 775930
SRR5031005 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031005 SRR5031005_1.fastq
Input file:	SRR5031005_1.fastq
trimmed:	SRR5031005-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:04:32 2020 >> started

Wed Mar  4 07:04:35 2020 >> done (2.367s)
4482906 reads processed; of these:
    218 ( 0.00%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
4482682 (100.00%) reads available; of these:
 250568 ( 5.59%) trimmed reads available after processing
4232114 (94.41%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     68	  0.00%
 19	    110	  0.00%
 20	    191	  0.00%
 21	    266	  0.01%
 22	    463	  0.01%
 23	    592	  0.01%
 24	    529	  0.01%
 25	    759	  0.02%
 26	    586	  0.01%
 27	    789	  0.02%
 28	    983	  0.02%
 29	   1739	  0.04%
 30	   1006	  0.02%
 31	    987	  0.02%
 32	   1208	  0.03%
 33	   1731	  0.04%
 34	   2305	  0.05%
 35	   4966	  0.11%
 36	   3817	  0.09%
 37	   4432	  0.10%
 38	   3837	  0.09%
 39	   3971	  0.09%
 40	   4529	  0.10%
 41	   4978	  0.11%
 42	   6445	  0.14%
 43	   9029	  0.20%
 44	  12140	  0.27%
 45	  15629	  0.35%
 46	  22232	  0.50%
 47	  29230	  0.65%
 48	  51134	  1.14%
 49	  59887	  1.34%
 50	4232114	 94.41%
4482682 reads passed initial QC


criterion=sequence-density
sequence-density=88.75
sequence-density-rank=1
fanout-score=19.50
fanout-score-rank=1
prefix-density=90.28
prefix-fanout=19.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC


criterion=fanout-score
sequence-density=88.75
sequence-density-rank=1
fanout-score=19.50
fanout-score-rank=1
prefix-density=90.28
prefix-fanout=19.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC -o SRR5031005 -
Input file:	STDIN
trimmed:	SRR5031005-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:04:56 2020 >> started

Wed Mar  4 07:05:03 2020 >> done (6.810s)
4381948 reads processed; of these:
 197222 ( 4.50%) short reads filtered out after trimming by size control
  22082 ( 0.50%) empty reads filtered out after trimming by size control
4162644 (95.00%) reads available; of these:
4000850 (96.11%) trimmed reads available after processing
 161794 ( 3.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  21119	  0.51%
 19	  53376	  1.28%
 20	 225811	  5.42%
 21	 497433	 11.95%
 22	1436394	 34.51%
 23	 727058	 17.47%
 24	 279002	  6.70%
 25	  46061	  1.11%
 26	  24004	  0.58%
 27	  22954	  0.55%
 28	  27173	  0.65%
 29	  21391	  0.51%
 30	  37182	  0.89%
 31	  56218	  1.35%
 32	  53318	  1.28%
 33	  79449	  1.91%
 34	  44194	  1.06%
 35	  30340	  0.73%
 36	  33396	  0.80%
 37	  20106	  0.48%
 38	  23121	  0.56%
 39	  20383	  0.49%
 40	  29125	  0.70%
 41	  39009	  0.94%
 42	  42351	  1.02%
 43	  31783	  0.76%
 44	  26185	  0.63%
 45	  32551	  0.78%
 46	  23742	  0.57%
 47	  16102	  0.39%
 48	   1646	  0.04%
 49	   3368	  0.08%
 50	 137299	  3.30%


criterion=sequence-density
sequence-density=5.79
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCTTTAACCTGTGAA


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=29
fanout-score=15.77
fanout-score-rank=1
prefix-density=6.57
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 07:05:25
                             Started mapping on |	Mar 04 07:05:25
                                    Finished on |	Mar 04 07:05:57
       Mapping speed, Million of reads per hour |	479.63

                          Number of input reads |	4263378
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2096706
                        Uniquely mapped reads % |	49.18%
                          Average mapped length |	24.29
                       Number of splices: Total |	2174
            Number of splices: Annotated (sjdb) |	205
                       Number of splices: GT/AG |	2004
                       Number of splices: GC/AG |	22
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	148
                      Mismatch rate per base, % |	0.14%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.81
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.15
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1716692
             % of reads mapped to multiple loci |	40.27%
        Number of reads mapped to too many loci |	103042
             % of reads mapped to too many loci |	2.42%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.41%
                     % of reads unmapped: other |	0.72%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	449980	449980	449980
N_multimapping	1716692	1716692	1716692
N_noFeature	89810	110238	1605203
N_ambiguous	1109391	693404	15621
UnstrandedReadsAssigned:897505 PositiveStrandReadsAssigned:1293064 NegativeStrandReadsAssigned:475882
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031005 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031005-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,263,378 reads, 831,375 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,160 rounds

 109283 SRR5031005.ke.tsv
  52636 SRR5031005.se.tsv
 161919 total
==> SRR5031005.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031005.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031005 completed mapping pipeline successfully
