Starting /dee2/code/volunteer_pipeline.sh SRR5031103
    current disk space = 160460439552
    free memory = 73952504 
SRR5031103 SRAfilesize
4cbe18c44639978dc5803995217b98c8  SRR5031103.sra
SRR5031103.sra file validated
SRR5031103 is single end
SRR5031103 is conventional basespace
SRR5031103 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90725	31.0	31.0	34.0	30.0	34.0
2	32.41525	33.0	31.0	34.0	31.0	34.0
3	32.461	34.0	31.0	34.0	31.0	34.0
4	36.1765	37.0	35.0	37.0	35.0	37.0
5	36.084	37.0	35.0	37.0	35.0	37.0
6	35.94275	37.0	35.0	37.0	35.0	37.0
7	35.66	37.0	35.0	37.0	35.0	37.0
8	35.52375	37.0	35.0	37.0	33.0	37.0
9	36.74275	39.0	37.0	39.0	32.0	39.0
10	36.181	39.0	35.0	39.0	30.0	39.0
11	37.28675	39.0	37.0	39.0	34.0	39.0
12	37.608	39.0	37.0	39.0	35.0	39.0
13	37.50125	39.0	37.0	39.0	35.0	39.0
14	38.24325	40.0	38.0	41.0	33.0	41.0
15	36.95475	40.0	36.0	41.0	30.0	41.0
16	36.8545	39.0	36.0	40.0	31.0	41.0
17	36.74425	39.0	36.0	40.0	31.0	41.0
18	38.13225	39.0	37.0	41.0	34.0	41.0
19	38.706	40.0	38.0	41.0	35.0	41.0
20	38.4435	40.0	38.0	41.0	34.0	41.0
21	38.45775	40.0	38.0	41.0	34.0	41.0
22	37.21325	39.0	36.0	40.0	31.0	41.0
23	37.75625	39.0	37.0	40.0	33.0	41.0
24	37.0375	39.0	36.0	40.0	31.0	41.0
25	37.9315	40.0	37.0	41.0	33.0	41.0
26	37.86825	40.0	37.0	41.0	33.0	41.0
27	38.2145	40.0	38.0	41.0	34.0	41.0
28	38.34725	40.0	38.0	41.0	34.0	41.0
29	38.1015	40.0	38.0	41.0	33.0	41.0
30	38.67425	40.0	38.0	41.0	34.0	41.0
31	38.59475	40.0	38.0	41.0	35.0	41.0
32	38.611	40.0	38.0	41.0	35.0	41.0
33	38.43175	40.0	38.0	41.0	34.0	41.0
34	37.7935	40.0	38.0	41.0	33.0	41.0
35	37.23225	40.0	36.0	41.0	30.0	41.0
36	33.4595	36.0	30.0	40.0	23.0	41.0
37	35.0275	37.0	33.0	39.0	30.0	40.0
38	36.45325	38.0	35.0	40.0	31.0	40.0
39	36.58125	38.0	35.0	40.0	31.0	40.0
40	36.8615	39.0	36.0	40.0	31.0	41.0
41	37.22625	39.0	37.0	40.0	32.0	41.0
42	37.268	39.0	37.0	40.0	32.0	41.0
43	37.368	40.0	37.0	41.0	32.0	41.0
44	36.7325	39.0	35.0	40.0	31.0	41.0
45	36.76575	39.0	36.0	40.0	30.0	41.0
46	36.756	39.0	36.0	40.0	30.0	41.0
47	35.6165	38.0	34.0	40.0	28.0	41.0
48	36.657	39.0	36.0	40.0	31.0	41.0
49	36.90875	40.0	36.0	41.0	32.0	41.0
50	35.17075	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.03340716131413757
1101	2	-0.16893184335044253
1101	3	-0.04740091949394554
1101	4	-0.07389509715091691
1101	5	-0.24485720997348892
1101	6	-0.10424846471358507
1101	7	0.06357595892480106
1101	8	-0.08473438705996728
1101	9	-0.2670223833014518
1101	10	-0.14644786737810023
1101	11	-0.2787006275378374
1101	12	-0.19388234504513235
1101	13	-0.46902580623510914
1101	14	-0.14052484982717317
1101	15	-0.5887445887445892
1101	16	-0.46387462666532997
1101	17	-0.8498942917547581
1101	18	-0.48521762475250796
1101	19	-0.28789556696533225
1101	20	0.012215175005877654
1101	21	-0.5774690425853208
1101	22	-0.19331185610255375
1101	23	0.16175039430853388
1101	24	-0.1853921272525909
1101	25	-0.44894123963891985
1101	26	-0.07240175844826524
1101	27	-0.06585791469512259
1101	28	-0.16814322628275846
1101	29	-0.36056579079835416
1101	30	-0.26271015805899367
1101	31	-0.1228229135205865
1101	32	-0.10914795798516508
1101	33	-0.06342494714588298
1101	34	0.1290647337159001
1101	35	-0.08015369643276671
1101	36	-0.5330212423235636
1101	37	-0.011426557938186477
1101	38	-0.32170542635658705
1101	39	-0.5081881942347053
1101	40	-0.5869492264841156
1101	41	-0.5177354944796804
1101	42	-0.34231014463572507
1101	43	-0.3808181482600048
1101	44	-0.5909426490821801
1101	45	-0.5660257055605911
1101	46	-0.1748380818148263
1101	47	-0.24405181381925445
1101	48	-0.45578710694989866
1101	49	-0.7321722205443137
1101	50	-0.6327729118426788
1102	1	0.03340716131413757
1102	2	0.16893184335044964
1102	3	0.04740091949393843
1102	4	0.0738950971509098
1102	5	0.24485720997348892
1102	6	0.10424846471357796
1102	7	-0.06357595892479395
1102	8	0.08473438705996728
1102	9	0.2670223833014518
1102	10	0.14644786737810023
1102	11	0.2787006275378374
1102	12	0.19388234504513946
1102	13	0.46902580623510914
1102	14	0.14052484982717317
1102	15	0.5887445887445892
1102	16	0.46387462666532286
1102	17	0.849894291754751
1102	18	0.48521762475250796
1102	19	0.28789556696533936
1102	20	-0.012215175005870549
1102	21	0.5774690425853208
1102	22	0.19331185610255375
1102	23	-0.16175039430853388
1102	24	0.1853921272525909
1102	25	0.44894123963891275
1102	26	0.07240175844827235
1102	27	0.06585791469512259
1102	28	0.16814322628276557
1102	29	0.36056579079834705
1102	30	0.2627101580590008
1102	31	0.1228229135205865
1102	32	0.10914795798516508
1102	33	0.06342494714587588
1102	34	-0.129064733715893
1102	35	0.08015369643276671
1102	36	0.5330212423235707
1102	37	0.011426557938186477
1102	38	0.32170542635658705
1102	39	0.5081881942347053
1102	40	0.5869492264841085
1102	41	0.5177354944796804
1102	42	0.34231014463572507
1102	43	0.3808181482600119
1102	44	0.5909426490821872
1102	45	0.566025705560584
1102	46	0.1748380818148263
1102	47	0.24405181381925445
1102	48	0.45578710694989866
1102	49	0.7321722205443137
1102	50	0.6327729118426788
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	2.0
16	2.0
17	0.0
18	4.0
19	3.0
20	3.0
21	4.0
22	5.0
23	3.0
24	11.0
25	6.0
26	16.0
27	22.0
28	29.0
29	40.0
30	55.0
31	71.0
32	96.0
33	144.0
34	215.0
35	242.0
36	448.0
37	743.0
38	1118.0
39	713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.200000000000001	25.924999999999997	51.075	14.799999999999999
2	24.718538904178132	29.171878909181885	16.012009006755065	30.09757317988491
3	24.224999999999998	19.7	19.075	37.0
4	34.150000000000006	27.650000000000002	13.25	24.95
5	25.275	35.3	28.349999999999998	11.075
6	32.4	12.475	37.85	17.275
7	13.375	25.55	22.55	38.525
8	27.775	26.700000000000003	14.85	30.675
9	21.55	34.225	28.075	16.150000000000002
10	19.05	18.5	28.475	33.975
11	35.725	10.325	14.6	39.35
12	17.849999999999998	17.8	28.825	35.525
13	30.225	14.45	36.425000000000004	18.9
14	31.2	27.1	23.275000000000002	18.425
15	35.375	17.474999999999998	27.800000000000004	19.35
16	19.975	17.925	21.45	40.65
17	27.6	22.275	34.375	15.75
18	24.675	15.825	30.825000000000003	28.675
19	35.75	14.475	29.625	20.150000000000002
20	47.3	10.925	29.549999999999997	12.225
21	26.25	18.75	27.625	27.375
22	28.225	11.25	48.475	12.049999999999999
23	25.45	12.675	48.875	13.0
24	51.449999999999996	11.3	26.375	10.875
25	53.0	23.799999999999997	15.15	8.05
26	28.7	47.699999999999996	12.975	10.625
27	16.55	50.175000000000004	22.875	10.4
28	11.85	26.200000000000003	48.825	13.125
29	12.4	10.925	57.05	19.625
30	9.45	6.6000000000000005	37.974999999999994	45.975
31	12.125	5.175	47.825	34.875
32	26.224999999999998	5.55	23.425	44.800000000000004
33	54.25	8.4	13.3	24.05
34	64.7	7.55	14.424999999999999	13.325000000000001
35	67.15	6.325	18.4	8.125
36	46.0	6.75	35.949999999999996	11.3
37	46.225	10.225	21.075	22.475
38	24.675	15.075	11.55	48.699999999999996
39	14.149999999999999	22.425	10.549999999999999	52.87500000000001
40	12.85	50.1	10.7	26.35
41	22.375	51.725	8.200000000000001	17.7
42	48.875	30.725	10.35	10.05
43	53.225	28.625	7.199999999999999	10.95
44	31.525	49.05	5.8500000000000005	13.575000000000001
45	17.349999999999998	52.325	12.525	17.8
46	12.475	27.0	17.549999999999997	42.975
47	7.825	14.424999999999999	39.875	37.875
48	9.35	12.174999999999999	21.85	56.625
49	11.125	20.25	12.375	56.25
50	16.2	37.55	12.7	33.550000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	1.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	1.0
28	0.0
29	1.5
30	3.0
31	4.0
32	5.0
33	7.0
34	9.0
35	11.0
36	13.0
37	17.5
38	22.0
39	19.0
40	16.0
41	26.5
42	37.0
43	60.0
44	83.0
45	111.5
46	140.0
47	260.0
48	380.0
49	466.0
50	552.0
51	747.5
52	943.0
53	726.0
54	509.0
55	519.0
56	529.0
57	421.0
58	313.0
59	223.5
60	134.0
61	96.5
62	59.0
63	95.5
64	132.0
65	94.5
66	57.0
67	36.5
68	16.0
69	13.5
70	11.0
71	15.0
72	19.0
73	13.0
74	7.0
75	4.5
76	2.0
77	2.0
78	2.0
79	2.0
80	2.0
81	1.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.075
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7957957957958	31.55
2	10.39039039039039	8.649999999999999
3	4.024024024024024	5.025
4	2.3423423423423424	3.9
5	1.5015015015015014	3.125
6	1.0210210210210209	2.55
7	0.7807807807807807	2.275
8	0.960960960960961	3.2
9	0.3003003003003003	1.125
>10	2.3423423423423424	18.675
>50	0.42042042042042044	13.450000000000001
>100	0.12012012012012012	6.4750000000000005
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	151	3.775	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	108	2.7	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	99	2.475	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	91	2.275	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	90	2.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	78	1.95	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	62	1.55	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	61	1.525	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	57	1.425	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	42	1.05	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	39	0.975	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 35bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGC	25	0.625	RNA PCR Primer, Index 27 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	25	0.625	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	22	0.5499999999999999	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 33bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGACCCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCCGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (96% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATT	12	0.3	RNA PCR Primer, Index 8 (97% over 36bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTC	12	0.3	RNA PCR Primer, Index 27 (100% over 44bp)
CGCGACCTCAGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTCGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCCGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGCTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGCACTCCAGTCACATTCCTATCTCGTATGC	7	0.17500000000000002	RNA PCR Primer, Index 27 (98% over 50bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	6	0.15	No Hit
CTTTTTGCGGTCTGGGCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	6	0.15	RNA PCR Primer, Index 1 (100% over 34bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGACTCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	5	0.125	RNA PCR Primer, Index 1 (100% over 35bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTCGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTAT	5	0.125	RNA PCR Primer, Index 27 (100% over 48bp)
ACGCCCTTCCCCCCCTTCTTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGCCCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTACCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGGCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.925	0.0	0.0	0.0
2	0.0	0.925	0.0	0.0	0.0
3	0.0	1.15	0.0	0.0	0.0
4	0.0	1.175	0.0	0.0	0.0
5	0.0	1.2	0.0	0.0	0.0
6	0.0	1.275	0.0	0.0	0.0
7	0.0	1.8	0.0	0.0	0.0
8	0.0	1.975	0.0	0.0	0.0
9	0.0	2.15	0.0	0.0	0.0
10	0.0	2.325	0.0	0.0	0.0
11	0.0	2.45	0.0	0.0	0.0
12	0.0	2.525	0.0	0.0	0.0
13	0.0	2.825	0.0	0.0	0.0
14	0.0	2.975	0.0	0.0	0.0
15	0.0	3.55	0.0	0.0	0.0
16	0.0	4.875	0.0	0.0	0.0
17	0.0	5.425	0.0	0.0	0.0
18	0.0	6.45	0.0	0.0	0.0
19	0.0	7.075	0.0	0.0	0.0
20	0.0	8.05	0.0	0.0	0.0
21	0.0	12.125	0.0	0.0	0.0
22	0.0	23.6	0.0	0.0	0.0
23	0.0	54.75	0.0	0.0	0.0
24	0.0	69.9	0.0	0.0	0.0
25	0.0	75.45	0.0	0.0	0.0
26	0.0	77.075	0.0	0.0	0.0
27	0.0	78.075	0.0	0.0	0.0
28	0.0	78.75	0.0	0.0	0.0
29	0.0	79.25	0.0	0.0	0.0
30	0.0	79.75	0.0	0.0	0.0
31	0.0	80.5	0.0	0.0	0.0
32	0.0	81.8	0.0	0.0	0.0
33	0.0	82.675	0.0	0.0	0.0
34	0.0	86.125	0.0	0.0	0.0
35	0.0	87.15	0.0	0.0	0.0
36	0.0	87.75	0.0	0.0	0.0
37	0.0	88.725	0.0	0.0	0.0
38	0.0	89.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	45	2.728484E-10	44.000004	2
GGTGAGT	45	2.728484E-10	44.000004	17
AATCCAG	20	6.952051E-4	44.000004	8
ATCCAGG	20	6.952051E-4	44.000004	9
TAGTTTG	20	6.952051E-4	44.000004	18
TCCAGGA	20	6.952051E-4	44.000004	10
AGTAATC	20	6.952051E-4	44.000004	5
GGCTTGG	20	6.952051E-4	44.000004	19
TAATCCA	20	6.952051E-4	44.000004	7
GGTAGTA	45	2.728484E-10	44.000004	4
TGAGGTA	45	2.728484E-10	44.000004	1
AGGTAGT	45	2.728484E-10	44.000004	3
CTGTTGG	20	6.952051E-4	44.000004	19
GTAATCC	20	6.952051E-4	44.000004	6
AGTTTGG	20	6.952051E-4	44.000004	19
GATAGGC	20	6.952051E-4	44.000004	15
GTAGTAG	45	2.728484E-10	44.000004	5
CCAGGAT	20	6.952051E-4	44.000004	11
AACGCTG	55	0.0	44.0	8
GTTTGGA	35	1.02281774E-7	44.0	20
>>END_MODULE
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
Read 36969 spots for SRR5031103.sra
Written 36969 spots for SRR5031103.sra
Read 36958 spots for SRR5031103.sra
Written 36958 spots for SRR5031103.sra
SRR ids: ['SRR5031103.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_n96ggw86
SRR5031103.sra spots: 443507
blocks: [[1, 36958], [36959, 73916], [73917, 110874], [110875, 147832], [147833, 184790], [184791, 221748], [221749, 258706], [258707, 295664], [295665, 332622], [332623, 369580], [369581, 406538], [406539, 443507]]
SRR5031103 file size 76336
SRR5031103 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031103 SRR5031103_1.fastq
Input file:	SRR5031103_1.fastq
trimmed:	SRR5031103-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:24:24 2020 >> started

Wed Mar  4 07:24:25 2020 >> done (0.408s)
443507 reads processed; of these:
    41 ( 0.01%) short reads filtered out after trimming by size control
     0 ( 0.00%) empty reads filtered out after trimming by size control
443466 (99.99%) reads available; of these:
 35756 ( 8.06%) trimmed reads available after processing
407710 (91.94%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    12	  0.00%
 19	    17	  0.00%
 20	    31	  0.01%
 21	    40	  0.01%
 22	    74	  0.02%
 23	   104	  0.02%
 24	   105	  0.02%
 25	   139	  0.03%
 26	   109	  0.02%
 27	   118	  0.03%
 28	   159	  0.04%
 29	   207	  0.05%
 30	   168	  0.04%
 31	   155	  0.03%
 32	   216	  0.05%
 33	   319	  0.07%
 34	   494	  0.11%
 35	  1108	  0.25%
 36	   696	  0.16%
 37	   622	  0.14%
 38	   734	  0.17%
 39	   747	  0.17%
 40	   727	  0.16%
 41	   833	  0.19%
 42	   981	  0.22%
 43	  1714	  0.39%
 44	  2010	  0.45%
 45	  2690	  0.61%
 46	  3260	  0.74%
 47	  2564	  0.58%
 48	  7186	  1.62%
 49	  7417	  1.67%
 50	407710	 91.94%
443466 reads passed initial QC


criterion=sequence-density
sequence-density=89.40
sequence-density-rank=1
fanout-score=21.33
fanout-score-rank=2
prefix-density=90.43
prefix-fanout=21.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC


criterion=fanout-score
sequence-density=3.41
sequence-density-rank=2
fanout-score=78.12
fanout-score-rank=1
prefix-density=89.73
prefix-fanout=3.0
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC -o SRR5031103 -
Input file:	STDIN
trimmed:	SRR5031103-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATTCCTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:24:30 2020 >> started

Wed Mar  4 07:24:30 2020 >> done (0.540s)
433611 reads processed; of these:
 30086 ( 6.94%) short reads filtered out after trimming by size control
  4691 ( 1.08%) empty reads filtered out after trimming by size control
398834 (91.98%) reads available; of these:
385086 (96.55%) trimmed reads available after processing
 13748 ( 3.45%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  2725	  0.68%
 19	  4691	  1.18%
 20	 21276	  5.33%
 21	 49369	 12.38%
 22	134341	 33.68%
 23	 66600	 16.70%
 24	 28655	  7.18%
 25	  8243	  2.07%
 26	  3960	  0.99%
 27	  2938	  0.74%
 28	  3084	  0.77%
 29	  2676	  0.67%
 30	  3296	  0.83%
 31	  6151	  1.54%
 32	  3931	  0.99%
 33	 15126	  3.79%
 34	  3653	  0.92%
 35	  3054	  0.77%
 36	  3908	  0.98%
 37	  1691	  0.42%
 38	  1740	  0.44%
 39	  1518	  0.38%
 40	  2432	  0.61%
 41	  2530	  0.63%
 42	  2875	  0.72%
 43	  1931	  0.48%
 44	  1338	  0.34%
 45	  1597	  0.40%
 46	  1160	  0.29%
 47	   944	  0.24%
 48	   153	  0.04%
 49	   270	  0.07%
 50	 10978	  2.75%


criterion=sequence-density
sequence-density=9.54
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=8
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=0.41
sequence-density-rank=36
fanout-score=25.53
fanout-score-rank=1
prefix-density=10.51
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 07:25:00
                             Started mapping on |	Mar 04 07:25:01
                                    Finished on |	Mar 04 07:25:24
       Mapping speed, Million of reads per hour |	63.97

                          Number of input reads |	408689
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	211725
                        Uniquely mapped reads % |	51.81%
                          Average mapped length |	23.95
                       Number of splices: Total |	758
            Number of splices: Annotated (sjdb) |	238
                       Number of splices: GT/AG |	745
                       Number of splices: GC/AG |	9
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	3
                      Mismatch rate per base, % |	0.35%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	149751
             % of reads mapped to multiple loci |	36.64%
        Number of reads mapped to too many loci |	6056
             % of reads mapped to too many loci |	1.48%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.23%
                     % of reads unmapped: other |	0.84%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	47213	47213	47213
N_multimapping	149751	149751	149751
N_noFeature	15718	17792	145919
N_ambiguous	117883	60322	1409
UnstrandedReadsAssigned:78124 PositiveStrandReadsAssigned:133611 NegativeStrandReadsAssigned:64397
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031103 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031103-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 408,689 reads, 101,347 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 908 rounds

 109283 SRR5031103.ke.tsv
  52636 SRR5031103.se.tsv
 161919 total
==> SRR5031103.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031103.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031103 completed mapping pipeline successfully
