Starting /dee2/code/volunteer_pipeline.sh SRR5030909
    current disk space = 160653508608
    free memory = 73947772 
SRR5030909 SRAfilesize
ef92d01c3d94088141622258fa4130ac  SRR5030909.sra
SRR5030909.sra file validated
SRR5030909 is single end
SRR5030909 is conventional basespace
SRR5030909 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53525	34.0	31.0	34.0	31.0	34.0
2	32.7335	34.0	31.0	34.0	31.0	34.0
3	32.85375	34.0	31.0	34.0	31.0	34.0
4	36.26825	37.0	37.0	37.0	35.0	37.0
5	36.323	37.0	37.0	37.0	35.0	37.0
6	36.13175	37.0	35.0	37.0	35.0	37.0
7	36.107	37.0	35.0	37.0	35.0	37.0
8	36.22475	37.0	36.0	37.0	35.0	37.0
9	37.9485	39.0	38.0	39.0	35.0	39.0
10	38.06975	39.0	39.0	39.0	37.0	39.0
11	37.92875	39.0	38.0	39.0	35.0	39.0
12	37.95375	39.0	38.0	39.0	35.0	39.0
13	37.8695	39.0	38.0	39.0	35.0	39.0
14	39.35575	41.0	39.0	41.0	36.0	41.0
15	39.32575	41.0	39.0	41.0	36.0	41.0
16	39.18775	41.0	39.0	41.0	36.0	41.0
17	39.43	41.0	39.0	41.0	36.0	41.0
18	39.35175	41.0	39.0	41.0	36.0	41.0
19	39.12175	40.0	39.0	41.0	36.0	41.0
20	39.20375	41.0	39.0	41.0	36.0	41.0
21	39.30125	41.0	39.0	41.0	36.0	41.0
22	39.26025	41.0	39.0	41.0	36.0	41.0
23	39.10475	41.0	39.0	41.0	36.0	41.0
24	38.83525	40.0	39.0	41.0	35.0	41.0
25	39.103	41.0	39.0	41.0	36.0	41.0
26	39.08175	40.0	39.0	41.0	36.0	41.0
27	39.048	40.0	39.0	41.0	35.0	41.0
28	39.0065	40.0	39.0	41.0	35.0	41.0
29	38.4595	40.0	38.0	41.0	34.0	41.0
30	38.71575	40.0	38.0	41.0	34.0	41.0
31	38.8635	40.0	38.0	41.0	35.0	41.0
32	39.07025	41.0	39.0	41.0	35.0	41.0
33	38.373	40.0	38.0	41.0	34.0	41.0
34	38.4345	40.0	38.0	41.0	33.0	41.0
35	38.18725	40.0	38.0	41.0	34.0	41.0
36	34.105	38.0	31.0	40.0	23.0	41.0
37	35.92425	38.0	35.0	40.0	30.0	41.0
38	37.17025	39.0	37.0	40.0	31.0	41.0
39	37.20475	39.0	37.0	40.0	31.0	41.0
40	37.2415	40.0	37.0	41.0	31.0	41.0
41	37.38775	40.0	37.0	41.0	32.0	41.0
42	37.60225	40.0	38.0	41.0	33.0	41.0
43	37.4585	40.0	37.0	41.0	32.0	41.0
44	37.09725	40.0	37.0	41.0	31.0	41.0
45	36.65525	39.0	36.0	40.0	31.0	41.0
46	35.12025	38.0	34.0	40.0	26.0	41.0
47	36.09025	39.0	35.0	40.0	30.0	41.0
48	36.527	39.0	37.0	41.0	31.0	41.0
49	35.445	39.0	35.0	41.0	28.0	41.0
50	30.352	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	5.0
19	1.0
20	6.0
21	5.0
22	6.0
23	13.0
24	11.0
25	2.0
26	23.0
27	22.0
28	29.0
29	34.0
30	43.0
31	62.0
32	86.0
33	111.0
34	175.0
35	175.0
36	257.0
37	480.0
38	1004.0
39	1449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.723169508525577	20.837512537612838	58.52557673019058	12.913741223671012
2	21.7	26.5	21.349999999999998	30.45
3	17.95	17.974999999999998	18.9	45.175
4	24.575	34.825	12.275	28.325
5	19.875	38.925	28.549999999999997	12.65
6	38.65	15.125	35.375	10.85
7	13.225000000000001	26.724999999999998	25.974999999999998	34.075
8	26.5	40.9	11.575000000000001	21.025
9	20.474999999999998	40.1	29.049999999999997	10.375
10	19.675	18.8	27.85	33.675
11	26.85	14.224999999999998	13.3	45.625
12	14.725	15.4	25.900000000000002	43.974999999999994
13	20.8	22.35	36.4	20.45
14	36.25	24.15	27.0	12.6
15	36.70917729432358	23.43085771442861	28.432108027006752	11.42785696424106
16	12.425	31.874999999999996	17.974999999999998	37.724999999999994
17	21.025	24.45	39.65	14.875
18	25.15	18.675	31.175000000000004	25.0
19	39.1	13.575000000000001	27.325	20.0
20	47.349999999999994	16.675	25.2	10.775
21	23.225	22.375	23.974999999999998	30.425
22	30.725	11.275	50.975	7.025
23	24.675	12.375	50.975	11.975
24	53.175	14.174999999999999	26.325	6.325
25	56.474999999999994	22.525000000000002	13.100000000000001	7.9
26	28.575	49.95	12.625	8.85
27	13.5	55.875	22.125	8.5
28	8.725	26.875	52.175000000000004	12.225
29	7.6	11.700000000000001	63.800000000000004	16.900000000000002
30	8.275	5.475	37.7	48.55
31	13.125	4.825	49.475	32.574999999999996
32	24.825	6.05	22.45	46.675
33	57.425000000000004	6.7	12.1	23.775
34	68.55	6.125	13.8	11.525
35	69.475	6.25	16.400000000000002	7.875
36	42.449999999999996	7.1	38.800000000000004	11.65
37	47.825	8.05	22.325	21.8
38	23.425	12.625	12.025	51.925
39	13.475000000000001	21.925	7.6499999999999995	56.95
40	13.175	51.525	8.225	27.075
41	22.75	56.775	7.375	13.100000000000001
42	53.300000000000004	32.175	7.875	6.65
43	57.074999999999996	28.449999999999996	7.025	7.449999999999999
44	29.375	51.975	6.25	12.4
45	13.8	58.325	11.1	16.775000000000002
46	7.375	29.575000000000003	17.525	45.525
47	6.3	13.825000000000001	43.375	36.5
48	8.225	11.450000000000001	21.65	58.675
49	11.55	18.3	12.55	57.599999999999994
50	17.1	40.825	11.625	30.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	3.0
28	6.0
29	4.5
30	3.0
31	9.0
32	15.0
33	15.5
34	16.0
35	28.0
36	40.0
37	42.5
38	45.0
39	45.0
40	45.0
41	42.5
42	40.0
43	85.5
44	131.0
45	174.5
46	218.0
47	407.0
48	596.0
49	621.5
50	647.0
51	810.5
52	974.0
53	668.0
54	362.0
55	367.5
56	373.0
57	339.5
58	306.0
59	177.0
60	48.0
61	41.0
62	34.0
63	47.0
64	60.0
65	38.0
66	16.0
67	12.0
68	8.0
69	4.5
70	1.0
71	5.5
72	10.0
73	6.0
74	2.0
75	1.5
76	1.0
77	1.5
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.025
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.025000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35449020931803	27.900000000000002
2	9.11546252532073	6.75
3	5.266711681296422	5.8500000000000005
4	1.688048615800135	2.5
5	1.350438892640108	2.5
6	1.350438892640108	3.0
7	0.4726536124240378	1.225
8	0.6076975016880486	1.7999999999999998
9	0.5401755570560433	1.7999999999999998
>10	3.848750844024308	26.55
>50	0.2025658338960162	5.4
>100	0.2025658338960162	14.725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	252	6.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	189	4.725	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	83	2.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	71	1.775	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	50	1.25	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	26	0.65	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	23	0.575	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	20	0.5	RNA PCR Primer, Index 1 (100% over 33bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTC	15	0.375	RNA PCR Primer, Index 35 (100% over 44bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	13	0.325	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	12	0.3	RNA PCR Primer, Index 7 (100% over 35bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGC	10	0.25	RNA PCR Primer, Index 35 (100% over 50bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	9	0.22499999999999998	No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	9	0.22499999999999998	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	9	0.22499999999999998	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGAAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	7	0.17500000000000002	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	7	0.17500000000000002	No Hit
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	6	0.15	No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	6	0.15	No Hit
GTCGATGATGAGAGCTTTGTTCTGAGCTGGAATTCTCGGGTGCCAAGGAA	6	0.15	RNA PCR Primer, Index 1 (100% over 23bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	6	0.15	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
CTTTTTGCGGTCTGGGCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCG	5	0.125	RNA PCR Primer, Index 1 (96% over 27bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.25	0.0	0.0	0.0
2	0.0	0.275	0.0	0.0	0.0
3	0.0	0.375	0.0	0.0	0.0
4	0.0	0.4	0.0	0.0	0.0
5	0.0	0.45	0.0	0.0	0.0
6	0.0	0.5	0.0	0.0	0.0
7	0.0	0.9	0.0	0.0	0.0
8	0.0	0.95	0.0	0.0	0.0
9	0.0	0.975	0.0	0.0	0.0
10	0.0	1.15	0.0	0.0	0.0
11	0.0	1.375	0.0	0.0	0.0
12	0.0	1.65	0.0	0.0	0.0
13	0.0	1.925	0.0	0.0	0.0
14	0.0	2.175	0.0	0.0	0.0
15	0.0	2.375	0.0	0.0	0.0
16	0.0	3.075	0.0	0.0	0.0
17	0.0	3.475	0.0	0.0	0.0
18	0.0	4.475	0.0	0.0	0.0
19	0.0	4.95	0.0	0.0	0.0
20	0.0	5.975	0.0	0.0	0.0
21	0.0	11.325	0.0	0.0	0.0
22	0.0	22.25	0.0	0.0	0.0
23	0.0	57.55	0.0	0.0	0.0
24	0.0	73.325	0.0	0.0	0.0
25	0.0	78.975	0.0	0.0	0.0
26	0.0	79.925	0.0	0.0	0.0
27	0.0	80.25	0.0	0.0	0.0
28	0.0	80.9	0.0	0.0	0.0
29	0.0	81.525	0.0	0.0	0.0
30	0.0	81.825	0.0	0.0	0.0
31	0.0	82.475	0.0	0.0	0.0
32	0.0	83.275	0.0	0.0	0.0
33	0.0	84.1	0.0	0.0	0.0
34	0.0	85.45	0.0	0.0	0.0
35	0.0	85.925	0.0	0.0	0.0
36	0.0	86.375	0.0	0.0	0.0
37	0.0	86.825	0.0	0.0	0.0
38	0.0	87.175	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	65	0.0	44.000004	5
CTGAGAC	65	0.0	44.000004	4
CTTTAAC	45	2.728484E-10	44.000004	12
CCTAACT	20	6.952051E-4	44.000004	11
CCTGAGA	65	0.0	44.000004	3
AACCTGT	45	2.728484E-10	44.000004	16
GTGAGTG	20	6.952051E-4	44.000004	18
ACCTGTT	40	5.315087E-9	44.000004	17
ACATCCT	20	6.952051E-4	44.000004	6
TAACCTG	45	2.728484E-10	44.000004	15
TAACTTG	20	6.952051E-4	44.000004	13
CTAACTT	20	6.952051E-4	44.000004	12
AGGCTTG	20	6.952051E-4	44.000004	18
ACCCTTT	45	2.728484E-10	44.000004	9
CCTTTAA	45	2.728484E-10	44.000004	11
CCCTGAG	65	0.0	44.000004	2
ACCCTAA	20	6.952051E-4	44.000004	9
GCTTGGC	20	6.952051E-4	44.000004	15
GACCCTT	45	2.728484E-10	44.000004	8
GACCCTA	20	6.952051E-4	44.000004	8
>>END_MODULE
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267191 spots for SRR5030909.sra
Written 267191 spots for SRR5030909.sra
Read 267198 spots for SRR5030909.sra
Written 267198 spots for SRR5030909.sra
SRR ids: ['SRR5030909.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_96mdhi0c
SRR5030909.sra spots: 3206299
blocks: [[1, 267191], [267192, 534382], [534383, 801573], [801574, 1068764], [1068765, 1335955], [1335956, 1603146], [1603147, 1870337], [1870338, 2137528], [2137529, 2404719], [2404720, 2671910], [2671911, 2939101], [2939102, 3206299]]
SRR5030909 file size 554647
SRR5030909 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030909 SRR5030909_1.fastq
Input file:	SRR5030909_1.fastq
trimmed:	SRR5030909-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:42:04 2020 >> started

Wed Mar  4 06:42:06 2020 >> done (1.898s)
3206299 reads processed; of these:
    117 ( 0.00%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
3206178 (100.00%) reads available; of these:
 172531 ( 5.38%) trimmed reads available after processing
3033647 (94.62%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     57	  0.00%
 19	     53	  0.00%
 20	    120	  0.00%
 21	    158	  0.00%
 22	    316	  0.01%
 23	    359	  0.01%
 24	    342	  0.01%
 25	    423	  0.01%
 26	    337	  0.01%
 27	    508	  0.02%
 28	    655	  0.02%
 29	   1184	  0.04%
 30	    690	  0.02%
 31	    842	  0.03%
 32	    754	  0.02%
 33	   1017	  0.03%
 34	   2598	  0.08%
 35	   2638	  0.08%
 36	   1855	  0.06%
 37	   2071	  0.06%
 38	   2168	  0.07%
 39	   2426	  0.08%
 40	   2588	  0.08%
 41	   3310	  0.10%
 42	   4155	  0.13%
 43	   5735	  0.18%
 44	  10659	  0.33%
 45	  10919	  0.34%
 46	   9854	  0.31%
 47	  14927	  0.47%
 48	  35428	  1.10%
 49	  53385	  1.67%
 50	3033647	 94.62%
3206178 reads passed initial QC


criterion=sequence-density
sequence-density=86.32
sequence-density-rank=1
fanout-score=16.89
fanout-score-rank=1
prefix-density=87.83
prefix-fanout=16.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGCC


criterion=fanout-score
sequence-density=86.32
sequence-density-rank=1
fanout-score=16.89
fanout-score-rank=1
prefix-density=87.83
prefix-fanout=16.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGCC -o SRR5030909 -
Input file:	STDIN
trimmed:	SRR5030909-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:42:21 2020 >> started

Wed Mar  4 06:42:26 2020 >> done (4.250s)
3132473 reads processed; of these:
 129475 ( 4.13%) short reads filtered out after trimming by size control
   8671 ( 0.28%) empty reads filtered out after trimming by size control
2994327 (95.59%) reads available; of these:
2766100 (92.38%) trimmed reads available after processing
 228227 ( 7.62%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  18701	  0.62%
 19	  34393	  1.15%
 20	 149836	  5.00%
 21	 356173	 11.89%
 22	1141564	 38.12%
 23	 468896	 15.66%
 24	 175042	  5.85%
 25	  31896	  1.07%
 26	  15369	  0.51%
 27	  13932	  0.47%
 28	  15561	  0.52%
 29	  11892	  0.40%
 30	  20158	  0.67%
 31	  25216	  0.84%
 32	  24976	  0.83%
 33	  46126	  1.54%
 34	  21817	  0.73%
 35	  15799	  0.53%
 36	  16001	  0.53%
 37	  11092	  0.37%
 38	  12944	  0.43%
 39	  12598	  0.42%
 40	  17391	  0.58%
 41	  23512	  0.79%
 42	  24611	  0.82%
 43	  18828	  0.63%
 44	  16235	  0.54%
 45	  17303	  0.58%
 46	  13091	  0.44%
 47	  10054	  0.34%
 48	   2552	  0.09%
 49	   9436	  0.32%
 50	 201332	  6.72%


criterion=sequence-density
sequence-density=7.57
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.46
sequence-density-rank=27
fanout-score=15.93
fanout-score-rank=1
prefix-density=7.34
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:42:46
                             Started mapping on |	Mar 04 06:42:47
                                    Finished on |	Mar 04 06:43:10
       Mapping speed, Million of reads per hour |	480.21

                          Number of input reads |	3068032
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1674567
                        Uniquely mapped reads % |	54.58%
                          Average mapped length |	25.92
                       Number of splices: Total |	1142
            Number of splices: Annotated (sjdb) |	161
                       Number of splices: GT/AG |	1023
                       Number of splices: GC/AG |	13
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	104
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.62
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1146095
             % of reads mapped to multiple loci |	37.36%
        Number of reads mapped to too many loci |	53538
             % of reads mapped to too many loci |	1.75%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.71%
                     % of reads unmapped: other |	0.61%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	247370	247370	247370
N_multimapping	1146095	1146095	1146095
N_noFeature	62713	75429	1283950
N_ambiguous	953429	622859	12247
UnstrandedReadsAssigned:658425 PositiveStrandReadsAssigned:976279 NegativeStrandReadsAssigned:378370
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030909 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030909-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,068,032 reads, 729,983 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,135 rounds

 109283 SRR5030909.ke.tsv
  52636 SRR5030909.se.tsv
 161919 total
==> SRR5030909.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030909.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030909 completed mapping pipeline successfully
