Starting /dee2/code/volunteer_pipeline.sh SRR5030914
    current disk space = 160639877120
    free memory = 56709284 
SRR5030914 SRAfilesize
690b8f623ad2669238ab969ef66dd350  SRR5030914.sra
SRR5030914.sra file validated
SRR5030914 is single end
SRR5030914 is conventional basespace
SRR5030914 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43125	34.0	31.0	34.0	31.0	34.0
2	32.7495	34.0	31.0	34.0	31.0	34.0
3	32.7195	34.0	31.0	34.0	31.0	34.0
4	36.274	37.0	37.0	37.0	35.0	37.0
5	36.30475	37.0	37.0	37.0	35.0	37.0
6	36.12425	37.0	36.0	37.0	35.0	37.0
7	36.0535	37.0	35.0	37.0	35.0	37.0
8	36.12125	37.0	36.0	37.0	35.0	37.0
9	37.96	39.0	38.0	39.0	35.0	39.0
10	37.8775	39.0	38.0	39.0	35.0	39.0
11	37.8805	39.0	38.0	39.0	35.0	39.0
12	37.6175	39.0	38.0	39.0	35.0	39.0
13	37.771	39.0	38.0	39.0	35.0	39.0
14	39.278	41.0	39.0	41.0	36.0	41.0
15	39.32675	41.0	39.0	41.0	36.0	41.0
16	39.17975	40.0	39.0	41.0	36.0	41.0
17	39.19475	41.0	39.0	41.0	36.0	41.0
18	39.309	41.0	39.0	41.0	36.0	41.0
19	39.0395	41.0	38.0	41.0	35.0	41.0
20	38.9865	41.0	39.0	41.0	36.0	41.0
21	39.056	40.0	39.0	41.0	36.0	41.0
22	39.3245	41.0	39.0	41.0	36.0	41.0
23	39.24775	41.0	39.0	41.0	36.0	41.0
24	38.638	40.0	38.0	41.0	34.0	41.0
25	38.59775	40.0	38.0	41.0	34.0	41.0
26	38.90625	41.0	39.0	41.0	35.0	41.0
27	38.958	41.0	39.0	41.0	35.0	41.0
28	38.905	40.0	39.0	41.0	35.0	41.0
29	38.402	40.0	38.0	41.0	33.0	41.0
30	38.6915	40.0	38.0	41.0	34.0	41.0
31	39.036	40.0	39.0	41.0	35.0	41.0
32	38.94875	41.0	39.0	41.0	35.0	41.0
33	37.817	40.0	38.0	41.0	32.0	41.0
34	37.85175	40.0	38.0	41.0	32.0	41.0
35	37.57175	40.0	37.0	41.0	32.0	41.0
36	32.17	37.0	26.0	40.0	15.0	41.0
37	34.98775	37.0	33.0	39.0	27.0	41.0
38	36.76025	39.0	37.0	40.0	31.0	41.0
39	37.12175	39.0	37.0	40.0	31.0	41.0
40	37.17125	39.0	37.0	40.0	32.0	41.0
41	36.843	39.0	36.0	40.0	30.0	41.0
42	36.95825	39.0	37.0	40.0	31.0	41.0
43	36.98025	39.0	37.0	41.0	31.0	41.0
44	37.271	40.0	37.0	41.0	32.0	41.0
45	36.872	40.0	36.0	41.0	31.0	41.0
46	34.6155	38.0	33.0	40.0	24.0	41.0
47	35.552	39.0	35.0	40.0	28.0	41.0
48	35.66475	39.0	35.0	40.0	28.0	41.0
49	34.83175	38.0	34.0	40.0	27.0	41.0
50	30.68775	34.0	28.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	3.0
17	1.0
18	2.0
19	2.0
20	9.0
21	6.0
22	10.0
23	9.0
24	14.0
25	19.0
26	25.0
27	27.0
28	30.0
29	35.0
30	49.0
31	74.0
32	95.0
33	106.0
34	149.0
35	201.0
36	278.0
37	527.0
38	1065.0
39	1263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.074222668004012	19.50852557673019	58.19959879638916	14.21765295887663
2	20.825	25.124999999999996	24.175	29.875
3	18.099999999999998	18.0	20.65	43.25
4	23.325000000000003	35.775	13.5	27.400000000000002
5	19.125	41.8	25.874999999999996	13.200000000000001
6	39.35	16.625	32.975	11.05
7	15.1	24.825	26.950000000000003	33.125
8	24.725	41.175	13.125	20.974999999999998
9	19.650000000000002	38.824999999999996	29.25	12.275
10	20.825	18.35	28.749999999999996	32.074999999999996
11	26.174999999999997	14.124999999999998	14.549999999999999	45.15
12	14.499999999999998	16.900000000000002	25.224999999999998	43.375
13	19.55	26.125	33.925	20.4
14	34.825	25.775	25.775	13.625000000000002
15	37.9	22.2	27.900000000000002	12.0
16	12.35	33.175	19.175	35.3
17	21.125	22.650000000000002	42.5	13.725000000000001
18	25.1	20.549999999999997	28.525	25.825
19	39.75	14.95	25.924999999999997	19.375
20	46.5	18.2	24.15	11.15
21	22.35	22.75	22.825	32.074999999999996
22	29.175	13.375	48.6	8.85
23	25.900000000000002	13.25	47.275	13.575000000000001
24	52.675000000000004	13.600000000000001	26.25	7.475
25	53.15	22.400000000000002	14.025000000000002	10.424999999999999
26	27.875	48.725	12.55	10.85
27	14.575	52.25	23.400000000000002	9.775
28	9.3	26.400000000000002	51.4	12.9
29	9.2	11.65	59.8	19.35
30	9.049999999999999	6.4	37.5	47.05
31	13.525	4.8	47.675	34.0
32	25.75	6.65	21.95	45.65
33	55.025	8.525	13.0	23.45
34	65.64999999999999	6.775	13.5	14.075
35	65.575	6.75	17.974999999999998	9.700000000000001
36	46.300000000000004	7.449999999999999	34.449999999999996	11.799999999999999
37	46.525	9.975000000000001	20.974999999999998	22.525000000000002
38	23.25	13.325000000000001	12.025	51.4
39	15.725	22.7	8.275	53.300000000000004
40	13.975000000000001	50.5	10.75	24.775
41	24.175	53.349999999999994	9.1	13.375
42	51.87500000000001	29.95	9.700000000000001	8.475000000000001
43	54.50000000000001	27.750000000000004	9.3	8.450000000000001
44	29.65	50.949999999999996	7.425	11.975
45	16.375	54.87499999999999	10.7	18.05
46	9.675	28.925	19.825	41.575
47	8.075000000000001	14.274999999999999	42.225	35.425000000000004
48	10.75	10.549999999999999	21.8	56.89999999999999
49	13.600000000000001	17.875	13.850000000000001	54.675
50	18.6046511627907	38.03450862715679	12.578144536134033	30.782695673918482
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.0
28	1.0
29	1.5
30	2.0
31	12.5
32	23.0
33	21.5
34	20.0
35	30.5
36	41.0
37	42.5
38	44.0
39	48.5
40	53.0
41	51.0
42	49.0
43	77.5
44	106.0
45	199.0
46	292.0
47	415.5
48	539.0
49	589.5
50	640.0
51	776.0
52	912.0
53	655.0
54	398.0
55	378.5
56	359.0
57	317.0
58	275.0
59	171.0
60	67.0
61	48.0
62	29.0
63	45.5
64	62.0
65	48.0
66	34.0
67	22.5
68	11.0
69	12.5
70	14.0
71	15.0
72	16.0
73	10.0
74	4.0
75	2.0
76	0.0
77	1.0
78	2.0
79	2.5
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23056994818653	29.425
2	8.743523316062177	6.75
3	3.6917098445595853	4.275
4	2.5906735751295336	4.0
5	1.6191709844559583	3.125
6	0.7124352331606217	1.6500000000000001
7	1.2305699481865284	3.325
8	0.6476683937823834	2.0
9	0.45336787564766834	1.575
>10	3.6917098445595853	27.35
>50	0.19430051813471502	5.1
>100	0.19430051813471502	11.425
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	195	4.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	160	4.0	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	102	2.55	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	75	1.875	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	47	1.175	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	45	1.125	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	29	0.7250000000000001	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	29	0.7250000000000001	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	28	0.7000000000000001	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGC	19	0.475	RNA PCR Primer, Index 21 (100% over 50bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC	17	0.42500000000000004	RNA PCR Primer, Index 21 (100% over 44bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	16	0.4	No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	12	0.3	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	12	0.3	RNA PCR Primer, Index 1 (97% over 35bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	9	0.22499999999999998	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	8	0.2	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	7	0.17500000000000002	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	6	0.15	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	6	0.15	No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
TAGCAGCACAGAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TTAAGGCACGCGGTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACAGAAATATTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCATGAACAAAGGTGATGACATAAAATTCATGCTCAATAGGATTACGCTG	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.525	0.0	0.0	0.0
2	0.0	0.55	0.0	0.0	0.0
3	0.0	0.7	0.0	0.0	0.0
4	0.0	0.7	0.0	0.0	0.0
5	0.0	0.75	0.0	0.0	0.0
6	0.0	0.775	0.0	0.0	0.0
7	0.0	1.3	0.0	0.0	0.0
8	0.0	1.375	0.0	0.0	0.0
9	0.0	1.425	0.0	0.0	0.0
10	0.0	1.55	0.0	0.0	0.0
11	0.0	1.675	0.0	0.0	0.0
12	0.0	1.9	0.0	0.0	0.0
13	0.0	2.075	0.0	0.0	0.0
14	0.0	2.175	0.0	0.0	0.0
15	0.0	2.6	0.0	0.0	0.0
16	0.0	3.2	0.0	0.0	0.0
17	0.0	3.7	0.0	0.0	0.0
18	0.0	4.6	0.0	0.0	0.0
19	0.0	5.3	0.0	0.0	0.0
20	0.0	6.4	0.0	0.0	0.0
21	0.0	10.9	0.0	0.0	0.0
22	0.0	22.325	0.0	0.0	0.0
23	0.0	54.875	0.0	0.0	0.0
24	0.0	68.975	0.0	0.0	0.0
25	0.0	74.375	0.0	0.0	0.0
26	0.0	75.25	0.0	0.0	0.0
27	0.0	75.65	0.0	0.0	0.0
28	0.0	75.975	0.0	0.0	0.0
29	0.0	76.35	0.0	0.0	0.0
30	0.0	76.6	0.0	0.0	0.0
31	0.0	77.025	0.0	0.0	0.0
32	0.0	77.45	0.0	0.0	0.0
33	0.0	78.2	0.0	0.0	0.0
34	0.0	80.15	0.0	0.0	0.0
35	0.0	80.925	0.0	0.0	0.0
36	0.0	81.425	0.0	0.0	0.0
37	0.0	81.975	0.0	0.0	0.0
38	0.0	82.525	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	45	2.728484E-10	44.000004	5
AACGCTG	20	6.952051E-4	44.000004	8
ACGCTGT	20	6.952051E-4	44.000004	9
CTGAGAC	45	2.728484E-10	44.000004	4
TAAACAT	20	6.952051E-4	44.000004	3
TTGTATA	20	6.952051E-4	44.000004	13
CTTTAAC	40	5.315087E-9	44.000004	12
AAACATC	20	6.952051E-4	44.000004	4
TTCAACG	20	6.952051E-4	44.000004	5
CGGGGGC	20	6.952051E-4	44.000004	32
CCTGAGA	45	2.728484E-10	44.000004	3
TCAACGC	20	6.952051E-4	44.000004	6
GTATAGT	20	6.952051E-4	44.000004	15
TAACCTG	40	5.315087E-9	44.000004	15
GGGGGCC	20	6.952051E-4	44.000004	33
GCTGTCG	20	6.952051E-4	44.000004	11
CCTTTAA	40	5.315087E-9	44.000004	11
CCCTGAG	45	2.728484E-10	44.000004	2
GGCCAAG	20	6.952051E-4	44.000004	36
GACTGGA	20	6.952051E-4	44.000004	14
>>END_MODULE
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533920 spots for SRR5030914.sra
Written 533920 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
Read 533914 spots for SRR5030914.sra
Written 533914 spots for SRR5030914.sra
SRR ids: ['SRR5030914.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_lf4d40dz
SRR5030914.sra spots: 6406974
blocks: [[1, 533914], [533915, 1067828], [1067829, 1601742], [1601743, 2135656], [2135657, 2669570], [2669571, 3203484], [3203485, 3737398], [3737399, 4271312], [4271313, 4805226], [4805227, 5339140], [5339141, 5873054], [5873055, 6406974]]
SRR5030914 file size 1109393
SRR5030914 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030914 SRR5030914_1.fastq
Input file:	SRR5030914_1.fastq
trimmed:	SRR5030914-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:43:32 2020 >> started

Wed Mar  4 06:43:35 2020 >> done (3.643s)
6406974 reads processed; of these:
    331 ( 0.01%) short reads filtered out after trimming by size control
      9 ( 0.00%) empty reads filtered out after trimming by size control
6406634 (99.99%) reads available; of these:
 497946 ( 7.77%) trimmed reads available after processing
5908688 (92.23%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     87	  0.00%
 19	    125	  0.00%
 20	    326	  0.01%
 21	    367	  0.01%
 22	    670	  0.01%
 23	    772	  0.01%
 24	    780	  0.01%
 25	    883	  0.01%
 26	    795	  0.01%
 27	   1130	  0.02%
 28	   1397	  0.02%
 29	   2169	  0.03%
 30	   1548	  0.02%
 31	   2104	  0.03%
 32	   2876	  0.04%
 33	   4893	  0.08%
 34	  18186	  0.28%
 35	   9068	  0.14%
 36	   6499	  0.10%
 37	   5924	  0.09%
 38	   9290	  0.15%
 39	   7615	  0.12%
 40	  13875	  0.22%
 41	   9517	  0.15%
 42	  17559	  0.27%
 43	  11939	  0.19%
 44	  38862	  0.61%
 45	  34140	  0.53%
 46	  29742	  0.46%
 47	  37453	  0.58%
 48	  98698	  1.54%
 49	 128657	  2.01%
 50	5908688	 92.23%
6406634 reads passed initial QC


criterion=sequence-density
sequence-density=82.56
sequence-density-rank=1
fanout-score=16.99
fanout-score-rank=2
prefix-density=84.32
prefix-fanout=16.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC


criterion=fanout-score
sequence-density=3.19
sequence-density-rank=4
fanout-score=76.90
fanout-score-rank=1
prefix-density=84.02
prefix-fanout=2.9
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC -o SRR5030914 -
Input file:	STDIN
trimmed:	SRR5030914-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:43:59 2020 >> started

Wed Mar  4 06:44:09 2020 >> done (10.125s)
6252257 reads processed; of these:
 246205 ( 3.94%) short reads filtered out after trimming by size control
  37070 ( 0.59%) empty reads filtered out after trimming by size control
5968982 (95.47%) reads available; of these:
5384475 (90.21%) trimmed reads available after processing
 584507 ( 9.79%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  33611	  0.56%
 19	  68015	  1.14%
 20	 312393	  5.23%
 21	 690535	 11.57%
 22	2152533	 36.06%
 23	 839531	 14.06%
 24	 336040	  5.63%
 25	  56866	  0.95%
 26	  27072	  0.45%
 27	  25268	  0.42%
 28	  28890	  0.48%
 29	  21684	  0.36%
 30	  39770	  0.67%
 31	  52319	  0.88%
 32	  53829	  0.90%
 33	  98240	  1.65%
 34	  51651	  0.87%
 35	  37863	  0.63%
 36	  44211	  0.74%
 37	  27714	  0.46%
 38	  29400	  0.49%
 39	  31463	  0.53%
 40	  42845	  0.72%
 41	  57709	  0.97%
 42	  64677	  1.08%
 43	  46154	  0.77%
 44	  44335	  0.74%
 45	  48592	  0.81%
 46	  39725	  0.67%
 47	  30662	  0.51%
 48	   7606	  0.13%
 49	  24949	  0.42%
 50	 502830	  8.42%


criterion=sequence-density
sequence-density=6.10
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.45
sequence-density-rank=29
fanout-score=15.64
fanout-score-rank=1
prefix-density=7.01
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:44:32
                             Started mapping on |	Mar 04 06:44:32
                                    Finished on |	Mar 04 06:45:18
       Mapping speed, Million of reads per hour |	479.22

                          Number of input reads |	6123359
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3345674
                        Uniquely mapped reads % |	54.64%
                          Average mapped length |	27.00
                       Number of splices: Total |	2184
            Number of splices: Annotated (sjdb) |	343
                       Number of splices: GT/AG |	1856
                       Number of splices: GC/AG |	30
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	298
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.45
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2230030
             % of reads mapped to multiple loci |	36.42%
        Number of reads mapped to too many loci |	128797
             % of reads mapped to too many loci |	2.10%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.25%
                     % of reads unmapped: other |	0.59%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	547655	547655	547655
N_multimapping	2230030	2230030	2230030
N_noFeature	130929	160280	2594107
N_ambiguous	1836855	1209743	28270
UnstrandedReadsAssigned:1377890 PositiveStrandReadsAssigned:1975651 NegativeStrandReadsAssigned:723297
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030914 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030914-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 6,123,359 reads, 1,403,985 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,205 rounds

 109283 SRR5030914.ke.tsv
  52636 SRR5030914.se.tsv
 161919 total
==> SRR5030914.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030914.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030914 completed mapping pipeline successfully
