Starting /dee2/code/volunteer_pipeline.sh SRR5030935
    current disk space = 160620396544
    free memory = 73955304 
SRR5030935 SRAfilesize
b597b57f55272a7f704457ea66d213d2  SRR5030935.sra
SRR5030935.sra file validated
SRR5030935 is single end
SRR5030935 is conventional basespace
SRR5030935 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.474	34.0	31.0	34.0	31.0	34.0
2	32.80125	34.0	31.0	34.0	31.0	34.0
3	32.7765	34.0	31.0	34.0	31.0	34.0
4	36.22825	37.0	37.0	37.0	35.0	37.0
5	36.2995	37.0	37.0	37.0	35.0	37.0
6	36.102	37.0	35.0	37.0	35.0	37.0
7	36.09625	37.0	35.0	37.0	35.0	37.0
8	36.179	37.0	36.0	37.0	35.0	37.0
9	37.941	39.0	38.0	39.0	35.0	39.0
10	37.922	39.0	38.0	39.0	35.0	39.0
11	37.849	39.0	38.0	39.0	35.0	39.0
12	37.768	39.0	38.0	39.0	35.0	39.0
13	37.75125	39.0	38.0	39.0	35.0	39.0
14	39.2465	41.0	39.0	41.0	36.0	41.0
15	39.269	41.0	39.0	41.0	36.0	41.0
16	39.23075	40.0	39.0	41.0	36.0	41.0
17	39.30925	41.0	39.0	41.0	36.0	41.0
18	39.41375	41.0	39.0	41.0	36.0	41.0
19	39.25425	41.0	39.0	41.0	36.0	41.0
20	39.098	41.0	39.0	41.0	36.0	41.0
21	39.122	40.0	39.0	41.0	36.0	41.0
22	39.28325	41.0	39.0	41.0	36.0	41.0
23	39.287	41.0	39.0	41.0	36.0	41.0
24	38.5505	40.0	38.0	41.0	34.0	41.0
25	38.758	40.0	38.0	41.0	34.0	41.0
26	38.94025	40.0	39.0	41.0	35.0	41.0
27	38.974	41.0	39.0	41.0	35.0	41.0
28	38.76575	40.0	38.0	41.0	34.0	41.0
29	37.94825	40.0	38.0	41.0	32.0	41.0
30	38.33025	40.0	38.0	41.0	34.0	41.0
31	38.89	40.0	38.0	41.0	35.0	41.0
32	38.839	40.0	38.0	41.0	35.0	41.0
33	37.77525	40.0	38.0	41.0	32.0	41.0
34	37.61225	40.0	37.0	41.0	31.0	41.0
35	37.54625	39.0	37.0	41.0	32.0	41.0
36	33.41	38.0	30.0	40.0	18.0	41.0
37	35.624	38.0	34.0	40.0	30.0	41.0
38	37.064	39.0	37.0	40.0	32.0	41.0
39	37.09075	39.0	37.0	40.0	32.0	41.0
40	37.1405	39.0	37.0	40.0	32.0	41.0
41	36.79075	39.0	36.0	40.0	31.0	41.0
42	36.809	39.0	36.0	40.0	31.0	41.0
43	36.807	39.0	36.0	40.0	31.0	41.0
44	36.98075	39.0	36.0	41.0	32.0	41.0
45	36.54475	39.0	36.0	40.0	30.0	41.0
46	34.19075	38.0	33.0	40.0	23.0	41.0
47	35.3755	38.0	35.0	40.0	28.0	41.0
48	35.6005	39.0	35.0	40.0	29.0	41.0
49	34.796	38.0	34.0	40.0	27.0	41.0
50	30.9045	34.0	28.0	39.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	3.0
19	3.0
20	3.0
21	9.0
22	4.0
23	10.0
24	18.0
25	19.0
26	20.0
27	24.0
28	42.0
29	40.0
30	38.0
31	75.0
32	65.0
33	114.0
34	167.0
35	219.0
36	316.0
37	537.0
38	1051.0
39	1221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.808856642481862	25.11883912934701	45.9344508381286	17.137853390042533
2	22.15	27.875	21.05	28.925
3	25.724999999999998	17.925	23.525	32.824999999999996
4	32.175	29.375	14.224999999999998	24.224999999999998
5	20.7	39.800000000000004	25.575	13.925
6	29.275000000000002	18.224999999999998	38.775	13.725000000000001
7	20.25	19.275000000000002	23.200000000000003	37.275000000000006
8	22.75	31.724999999999998	15.275	30.25
9	23.549999999999997	32.550000000000004	27.625	16.275000000000002
10	24.625	18.8	31.2	25.374999999999996
11	31.775	14.625	17.724999999999998	35.875
12	14.774999999999999	21.6	31.7	31.924999999999997
13	28.1	19.0	31.15	21.75
14	28.475	31.35	22.075	18.099999999999998
15	33.35	22.95	27.625	16.075
16	17.7	21.55	22.775000000000002	37.974999999999994
17	25.7	23.65	36.5	14.149999999999999
18	26.424999999999997	18.125	31.424999999999997	24.025
19	33.050000000000004	16.625	31.7	18.625
20	40.725	18.075	25.174999999999997	16.025
21	24.4	20.599999999999998	25.45	29.549999999999997
22	27.900000000000002	15.125	43.974999999999994	13.0
23	26.125	13.725000000000001	41.3	18.85
24	48.6	15.950000000000001	25.275	10.174999999999999
25	47.9	23.1	15.75	13.25
26	27.275	43.175000000000004	15.375	14.174999999999999
27	18.275	43.35	24.325	14.05
28	15.0	24.3	44.775	15.925
29	11.725	16.45	52.349999999999994	19.475
30	11.475	9.45	34.0	45.074999999999996
31	16.0	8.075000000000001	43.85	32.074999999999996
32	27.700000000000003	8.75	21.925	41.625
33	51.275000000000006	11.025	14.6	23.1
34	55.2	11.4	17.25	16.150000000000002
35	58.75	9.925	18.725	12.6
36	43.075	10.65	31.4	14.875
37	42.65	12.025	20.25	25.074999999999996
38	23.325000000000003	16.875	14.7	45.1
39	18.275	23.175	11.675	46.875
40	17.575	44.1	14.825	23.5
41	25.624999999999996	45.1	11.899999999999999	17.375
42	47.349999999999994	28.475	12.75	11.425
43	47.375	30.349999999999998	11.425	10.85
44	30.599999999999998	46.075	8.975	14.35
45	19.3	48.125	15.174999999999999	17.4
46	14.549999999999999	26.8	20.1	38.550000000000004
47	11.325000000000001	17.95	36.925000000000004	33.800000000000004
48	12.35	15.225	19.775000000000002	52.65
49	15.299999999999999	21.15	17.125	46.425
50	18.925	35.975	16.375	28.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	1.0
22	1.0
23	0.5
24	0.0
25	1.5
26	3.0
27	2.5
28	2.0
29	4.5
30	7.0
31	13.5
32	20.0
33	23.0
34	26.0
35	39.0
36	52.0
37	68.0
38	84.0
39	83.5
40	83.0
41	71.5
42	60.0
43	101.0
44	142.0
45	177.0
46	212.0
47	294.5
48	377.0
49	504.5
50	632.0
51	720.5
52	809.0
53	624.5
54	440.0
55	441.5
56	443.0
57	335.0
58	227.0
59	146.5
60	66.0
61	59.5
62	53.0
63	76.0
64	99.0
65	68.5
66	38.0
67	26.0
68	14.0
69	17.0
70	20.0
71	42.0
72	64.0
73	35.5
74	7.0
75	4.5
76	2.0
77	4.5
78	7.0
79	5.5
80	4.0
81	3.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.74468085106383	31.15
2	8.632218844984802	7.1
3	4.0729483282674765	5.025
4	2.674772036474164	4.3999999999999995
5	1.82370820668693	3.75
6	1.5197568389057752	3.75
7	0.911854103343465	2.625
8	0.547112462006079	1.7999999999999998
9	0.303951367781155	1.125
>10	3.343465045592705	24.675
>50	0.303951367781155	9.3
>100	0.12158054711246201	5.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	108	2.7	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	104	2.6	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	93	2.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	82	2.0500000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	71	1.775	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	64	1.6	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	49	1.225	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	47	1.175	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	31	0.775	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	25	0.625	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	24	0.6	RNA PCR Primer, Index 7 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC	21	0.525	RNA PCR Primer, Index 42 (100% over 44bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	21	0.525	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	20	0.5	No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	19	0.475	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	18	0.44999999999999996	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	17	0.42500000000000004	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	15	0.375	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC	14	0.35000000000000003	RNA PCR Primer, Index 42 (100% over 50bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	13	0.325	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	13	0.325	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	12	0.3	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	12	0.3	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	12	0.3	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGAAGAAAGGT	12	0.3	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	11	0.27499999999999997	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	11	0.27499999999999997	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	11	0.27499999999999997	No Hit
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	11	0.27499999999999997	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	9	0.22499999999999998	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	8	0.2	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGGGTGCCAAGGAAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 24bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	7	0.17500000000000002	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	7	0.17500000000000002	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
CTCAATAGGATTACGCTGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	6	0.15	No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	6	0.15	RNA PCR Primer, Index 7 (97% over 35bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTACAGTACTGTGATAACTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT	5	0.125	RNA PCR Primer, Index 42 (100% over 37bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAAT	5	0.125	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
CGCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	5	0.125	No Hit
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.35	0.0	0.0	0.0
2	0.0	0.375	0.0	0.0	0.0
3	0.0	0.475	0.0	0.0	0.0
4	0.0	0.475	0.0	0.0	0.0
5	0.0	0.6	0.0	0.0	0.0
6	0.0	0.75	0.0	0.0	0.0
7	0.0	1.4	0.0	0.0	0.0
8	0.0	1.575	0.0	0.0	0.0
9	0.0	1.675	0.0	0.0	0.0
10	0.0	1.875	0.0	0.0	0.0
11	0.0	2.225	0.0	0.0	0.0
12	0.0	2.45	0.0	0.0	0.0
13	0.0	2.75	0.0	0.0	0.0
14	0.0	3.35	0.0	0.0	0.0
15	0.0	3.575	0.0	0.0	0.0
16	0.0	5.0	0.0	0.0	0.0
17	0.0	5.575	0.0	0.0	0.0
18	0.0	6.475	0.0	0.0	0.0
19	0.0	6.9	0.0	0.0	0.0
20	0.0	7.65	0.0	0.0	0.0
21	0.0	12.525	0.0	0.0	0.0
22	0.0	22.5	0.0	0.0	0.0
23	0.0	48.275	0.0	0.0	0.0
24	0.0	59.05	0.0	0.0	0.0
25	0.0	64.6	0.0	0.0	0.0
26	0.0	66.175	0.0	0.0	0.0
27	0.0	67.05	0.0	0.0	0.0
28	0.0	67.575	0.0	0.0	0.0
29	0.0	68.125	0.0	0.0	0.0
30	0.0	68.675	0.0	0.0	0.0
31	0.0	69.45	0.0	0.0	0.0
32	0.0	70.9	0.0	0.0	0.0
33	0.0	71.775	0.0	0.0	0.0
34	0.0	74.05	0.0	0.0	0.0
35	0.0	74.95	0.0	0.0	0.0
36	0.0	75.525	0.0	0.0	0.0
37	0.0	76.275	0.0	0.0	0.0
38	0.0	76.725	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTAG	20	6.952051E-4	44.000004	11
ATCCTAG	20	6.952051E-4	44.000004	5
AATCCTA	20	6.952051E-4	44.000004	4
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
TCCTAGC	20	6.952051E-4	44.000004	6
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
GGATAGG	20	6.952051E-4	44.000004	14
CTAATCC	20	6.952051E-4	44.000004	2
GGCTTGG	20	6.952051E-4	44.000004	19
TAATCCT	20	6.952051E-4	44.000004	3
GCTTGGC	20	6.952051E-4	44.000004	15
CAGGATA	20	6.952051E-4	44.000004	12
CCCTAGC	20	6.952051E-4	44.000004	12
CAAGTAA	20	6.952051E-4	44.000004	3
AACGCTG	30	1.9515464E-6	44.0	8
GGTGAGT	30	1.9515464E-6	44.0	17
ACGCTGT	30	1.9515464E-6	44.0	9
TAAACAT	35	1.02281774E-7	44.0	3
>>END_MODULE
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222519 spots for SRR5030935.sra
Written 222519 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
Read 222509 spots for SRR5030935.sra
Written 222509 spots for SRR5030935.sra
SRR ids: ['SRR5030935.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_23qmug91
SRR5030935.sra spots: 2670118
blocks: [[1, 222509], [222510, 445018], [445019, 667527], [667528, 890036], [890037, 1112545], [1112546, 1335054], [1335055, 1557563], [1557564, 1780072], [1780073, 2002581], [2002582, 2225090], [2225091, 2447599], [2447600, 2670118]]
SRR5030935 file size 461710
SRR5030935 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030935 SRR5030935_1.fastq
Input file:	SRR5030935_1.fastq
trimmed:	SRR5030935-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:48:39 2020 >> started

Wed Mar  4 06:48:41 2020 >> done (1.590s)
2670118 reads processed; of these:
    288 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2669824 (99.99%) reads available; of these:
 197350 ( 7.39%) trimmed reads available after processing
2472474 (92.61%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     68	  0.00%
 19	     72	  0.00%
 20	    130	  0.00%
 21	    143	  0.01%
 22	    269	  0.01%
 23	    361	  0.01%
 24	    362	  0.01%
 25	    386	  0.01%
 26	    382	  0.01%
 27	    516	  0.02%
 28	    586	  0.02%
 29	    911	  0.03%
 30	    681	  0.03%
 31	    958	  0.04%
 32	   1173	  0.04%
 33	   2155	  0.08%
 34	   6543	  0.25%
 35	   3403	  0.13%
 36	   2414	  0.09%
 37	   2543	  0.10%
 38	   3742	  0.14%
 39	   3458	  0.13%
 40	   5505	  0.21%
 41	   4000	  0.15%
 42	   6697	  0.25%
 43	   5461	  0.20%
 44	  14634	  0.55%
 45	  13646	  0.51%
 46	  12928	  0.48%
 47	  16358	  0.61%
 48	  35699	  1.34%
 49	  51166	  1.92%
 50	2472474	 92.61%
2669824 reads passed initial QC


criterion=sequence-density
sequence-density=76.34
sequence-density-rank=1
fanout-score=21.33
fanout-score-rank=2
prefix-density=78.66
prefix-fanout=20.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC


criterion=fanout-score
sequence-density=2.23
sequence-density-rank=4
fanout-score=109.08
fanout-score-rank=1
prefix-density=78.00
prefix-fanout=3.1
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC -o SRR5030935 -
Input file:	STDIN
trimmed:	SRR5030935-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:48:54 2020 >> started

Wed Mar  4 06:48:59 2020 >> done (4.174s)
2600478 reads processed; of these:
 144948 ( 5.57%) short reads filtered out after trimming by size control
  11900 ( 0.46%) empty reads filtered out after trimming by size control
2443630 (93.97%) reads available; of these:
2104433 (86.12%) trimmed reads available after processing
 339197 (13.88%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  11917	  0.49%
 19	  20195	  0.83%
 20	 130160	  5.33%
 21	 265839	 10.88%
 22	 679312	 27.80%
 23	 294687	 12.06%
 24	 143610	  5.88%
 25	  38836	  1.59%
 26	  18935	  0.77%
 27	  14987	  0.61%
 28	  16013	  0.66%
 29	  12587	  0.52%
 30	  19920	  0.82%
 31	  45151	  1.85%
 32	  25458	  1.04%
 33	  57822	  2.37%
 34	  24978	  1.02%
 35	  20867	  0.85%
 36	  22972	  0.94%
 37	  14669	  0.60%
 38	  16016	  0.66%
 39	  16361	  0.67%
 40	  24558	  1.00%
 41	  31446	  1.29%
 42	  37834	  1.55%
 43	  27283	  1.12%
 44	  22540	  0.92%
 45	  32343	  1.32%
 46	  23684	  0.97%
 47	  19594	  0.80%
 48	   3779	  0.15%
 49	  10472	  0.43%
 50	 298805	 12.23%


criterion=sequence-density
sequence-density=2.87
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AAGCTGCCAGTTGAAGAACTGT


criterion=fanout-score
sequence-density=0.69
sequence-density-rank=15
fanout-score=11.38
fanout-score-rank=1
prefix-density=7.84
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:49:21
                             Started mapping on |	Mar 04 06:49:22
                                    Finished on |	Mar 04 06:49:45
       Mapping speed, Million of reads per hour |	393.34

                          Number of input reads |	2512976
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1481025
                        Uniquely mapped reads % |	58.94%
                          Average mapped length |	28.02
                       Number of splices: Total |	1217
            Number of splices: Annotated (sjdb) |	196
                       Number of splices: GT/AG |	1136
                       Number of splices: GC/AG |	14
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	67
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.50
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	700845
             % of reads mapped to multiple loci |	27.89%
        Number of reads mapped to too many loci |	60675
             % of reads mapped to too many loci |	2.41%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.84%
                     % of reads unmapped: other |	0.93%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	331106	331106	331106
N_multimapping	700845	700845	700845
N_noFeature	92659	113964	1036430
N_ambiguous	808096	420239	15327
UnstrandedReadsAssigned:580270 PositiveStrandReadsAssigned:946822 NegativeStrandReadsAssigned:429268
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030935 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030935-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,512,976 reads, 646,710 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,088 rounds

 109283 SRR5030935.ke.tsv
  52636 SRR5030935.se.tsv
 161919 total
==> SRR5030935.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030935.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030935 completed mapping pipeline successfully
