Starting /dee2/code/volunteer_pipeline.sh SRR5030907
    current disk space = 160648380416
    free memory = 73945732 
SRR5030907 SRAfilesize
518908343b9e9b29003e58c5feb1a5ed  SRR5030907.sra
SRR5030907.sra file validated
SRR5030907 is single end
SRR5030907 is conventional basespace
SRR5030907 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4765	33.0	31.0	34.0	31.0	34.0
2	32.733	34.0	31.0	34.0	31.0	34.0
3	32.84225	34.0	31.0	34.0	31.0	34.0
4	36.29975	37.0	37.0	37.0	35.0	37.0
5	36.31425	37.0	37.0	37.0	35.0	37.0
6	36.094	37.0	35.0	37.0	35.0	37.0
7	36.09675	37.0	35.0	37.0	35.0	37.0
8	36.223	37.0	36.0	37.0	35.0	37.0
9	37.9345	39.0	38.0	39.0	35.0	39.0
10	38.03825	39.0	38.0	39.0	35.0	39.0
11	37.88625	39.0	38.0	39.0	35.0	39.0
12	37.85025	39.0	38.0	39.0	35.0	39.0
13	37.82025	39.0	38.0	39.0	35.0	39.0
14	39.317	41.0	39.0	41.0	36.0	41.0
15	39.3105	41.0	39.0	41.0	36.0	41.0
16	39.292	40.0	39.0	41.0	36.0	41.0
17	39.4675	41.0	39.0	41.0	36.0	41.0
18	39.3905	41.0	39.0	41.0	36.0	41.0
19	38.87425	40.0	38.0	41.0	35.0	41.0
20	39.0545	40.0	39.0	41.0	36.0	41.0
21	39.235	40.0	39.0	41.0	36.0	41.0
22	39.23575	41.0	39.0	41.0	36.0	41.0
23	39.05825	41.0	39.0	41.0	36.0	41.0
24	38.894	40.0	39.0	41.0	35.0	41.0
25	39.1605	41.0	39.0	41.0	36.0	41.0
26	39.13725	41.0	39.0	41.0	36.0	41.0
27	39.21125	41.0	39.0	41.0	36.0	41.0
28	39.09025	41.0	39.0	41.0	35.0	41.0
29	38.329	40.0	38.0	41.0	34.0	41.0
30	38.73	40.0	38.0	41.0	34.0	41.0
31	38.934	41.0	39.0	41.0	35.0	41.0
32	39.14375	41.0	39.0	41.0	36.0	41.0
33	38.50375	40.0	38.0	41.0	34.0	41.0
34	38.532	40.0	38.0	41.0	34.0	41.0
35	38.1405	40.0	38.0	41.0	34.0	41.0
36	33.50175	38.0	30.0	40.0	18.0	41.0
37	35.58525	38.0	34.0	40.0	30.0	41.0
38	37.06925	39.0	37.0	40.0	32.0	41.0
39	36.973	39.0	37.0	40.0	31.0	41.0
40	37.08975	39.0	37.0	41.0	31.0	41.0
41	37.2115	39.0	37.0	41.0	31.0	41.0
42	37.412	40.0	37.0	41.0	32.0	41.0
43	37.2465	40.0	37.0	41.0	32.0	41.0
44	37.11075	40.0	37.0	41.0	32.0	41.0
45	36.66025	39.0	36.0	41.0	30.0	41.0
46	34.90175	38.0	34.0	40.0	24.0	41.0
47	35.82525	39.0	35.0	40.0	29.0	41.0
48	36.317	39.0	36.0	41.0	30.0	41.0
49	35.27425	39.0	35.0	40.0	28.0	41.0
50	30.1	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	1.0
18	2.0
19	5.0
20	6.0
21	5.0
22	4.0
23	10.0
24	12.0
25	14.0
26	15.0
27	17.0
28	30.0
29	39.0
30	39.0
31	70.0
32	72.0
33	129.0
34	133.0
35	200.0
36	316.0
37	478.0
38	1079.0
39	1321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.18648310387985	20.77596996245307	55.14392991239049	14.893617021276595
2	22.425	27.150000000000002	22.225	28.199999999999996
3	19.25	18.775	17.8	44.175
4	26.400000000000002	33.875	13.275	26.450000000000003
5	22.475	38.550000000000004	26.700000000000003	12.275
6	36.7	15.299999999999999	36.1	11.899999999999999
7	13.325000000000001	26.400000000000002	25.124999999999996	35.15
8	25.974999999999998	39.225	12.975	21.825
9	20.474999999999998	37.7	29.275000000000002	12.55
10	21.7	19.975	26.275	32.05
11	30.275000000000002	14.45	13.750000000000002	41.525
12	16.0	17.75	23.625	42.625
13	20.65	22.400000000000002	35.8	21.15
14	34.949999999999996	24.325	27.125	13.600000000000001
15	37.918959479739875	22.461230615307652	27.613806903451728	12.006003001500751
16	12.9	28.599999999999998	21.125	37.375
17	24.025	22.95	38.475	14.549999999999999
18	25.8	18.325	30.099999999999998	25.775
19	38.025	14.2	28.725	19.05
20	49.725	16.025	22.400000000000002	11.85
21	23.150000000000002	24.65	22.575	29.625
22	31.275	11.85	48.125	8.75
23	24.925	11.825	50.275000000000006	12.975
24	54.1	12.425	26.525	6.950000000000001
25	56.25	21.45	13.425	8.875
26	27.575	50.324999999999996	12.45	9.65
27	13.525	54.2	21.825	10.45
28	9.325	25.7	52.2	12.775
29	9.5	11.325000000000001	61.525	17.65
30	9.5	5.25	36.3	48.949999999999996
31	13.3	4.5249999999999995	48.75	33.425
32	25.3	5.800000000000001	22.025	46.875
33	57.05	6.875000000000001	11.899999999999999	24.175
34	68.075	6.65	13.0	12.275
35	67.15	7.225	16.975	8.649999999999999
36	42.9	7.025	38.275	11.799999999999999
37	47.875	8.9	22.275	20.95
38	23.25	13.8	11.700000000000001	51.24999999999999
39	14.000000000000002	21.9	7.775	56.325
40	13.4	51.625	9.6	25.374999999999996
41	22.650000000000002	55.775	8.0	13.575000000000001
42	53.5	30.65	8.5	7.35
43	57.875	26.450000000000003	7.3999999999999995	8.275
44	29.849999999999998	51.74999999999999	6.65	11.75
45	15.925	56.425000000000004	10.0	17.65
46	9.975000000000001	28.225	17.125	44.675
47	7.9	13.825000000000001	42.225	36.05
48	9.4	10.85	22.1	57.65
49	13.475000000000001	17.9	11.625	56.99999999999999
50	18.7	40.699999999999996	11.0	29.599999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	0.0
29	0.5
30	1.0
31	4.5
32	8.0
33	14.0
34	20.0
35	19.5
36	19.0
37	33.5
38	48.0
39	43.5
40	39.0
41	43.0
42	47.0
43	79.5
44	112.0
45	164.5
46	217.0
47	371.0
48	525.0
49	557.0
50	589.0
51	784.5
52	980.0
53	686.0
54	392.0
55	392.0
56	392.0
57	360.0
58	328.0
59	193.0
60	58.0
61	49.0
62	40.0
63	65.5
64	91.0
65	63.0
66	35.0
67	19.5
68	4.0
69	9.0
70	14.0
71	20.5
72	27.0
73	15.0
74	3.0
75	3.0
76	3.0
77	3.0
78	3.0
79	2.5
80	2.0
81	1.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.05
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64599483204134	29.275000000000002
2	9.754521963824288	7.55
3	4.651162790697675	5.4
4	1.8733850129198968	2.9000000000000004
5	1.614987080103359	3.125
6	0.9043927648578811	2.1
7	0.6459948320413437	1.7500000000000002
8	0.6459948320413437	2.0
9	0.32299741602067183	1.125
>10	3.6175710594315245	27.500000000000004
>50	0.06459948320413438	1.5
>100	0.2583979328165375	15.775
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	202	5.050000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	164	4.1000000000000005	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	138	3.45	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	127	3.175	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	60	1.5	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	48	1.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	38	0.95	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	34	0.8500000000000001	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	31	0.775	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	20	0.5	RNA PCR Primer, Index 1 (97% over 35bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTC	18	0.44999999999999996	RNA PCR Primer, Index 17 (100% over 44bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13	0.325	RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	13	0.325	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC	13	0.325	RNA PCR Primer, Index 17 (100% over 50bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 32bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	10	0.25	No Hit
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	8	0.2	No Hit
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	8	0.2	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACAGAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	6	0.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	6	0.15	No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	6	0.15	No Hit
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGCAACTCTGTGCTGAATGT	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	5	0.125	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.325	0.0	0.0	0.0
2	0.0	0.325	0.0	0.0	0.0
3	0.0	0.425	0.0	0.0	0.0
4	0.0	0.45	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.625	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.225	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.55	0.0	0.0	0.0
11	0.0	1.75	0.0	0.0	0.0
12	0.0	1.9	0.0	0.0	0.0
13	0.0	2.275	0.0	0.0	0.0
14	0.0	2.6	0.0	0.0	0.0
15	0.0	2.825	0.0	0.0	0.0
16	0.0	3.65	0.0	0.0	0.0
17	0.0	4.1	0.0	0.0	0.0
18	0.0	4.9	0.0	0.0	0.0
19	0.0	5.55	0.0	0.0	0.0
20	0.0	6.725	0.0	0.0	0.0
21	0.0	11.15	0.0	0.0	0.0
22	0.0	21.775	0.0	0.0	0.0
23	0.0	56.45	0.0	0.0	0.0
24	0.0	71.7	0.0	0.0	0.0
25	0.0	76.825	0.0	0.0	0.0
26	0.0	77.85	0.0	0.0	0.0
27	0.0	78.175	0.0	0.0	0.0
28	0.0	78.525	0.0	0.0	0.0
29	0.0	79.125	0.0	0.0	0.0
30	0.0	79.675	0.0	0.0	0.0
31	0.0	80.45	0.0	0.0	0.0
32	0.0	81.475	0.0	0.0	0.0
33	0.0	82.3	0.0	0.0	0.0
34	0.0	84.1	0.0	0.0	0.0
35	0.0	85.075	0.0	0.0	0.0
36	0.0	85.725	0.0	0.0	0.0
37	0.0	86.275	0.0	0.0	0.0
38	0.0	86.775	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCCG	20	6.952051E-4	44.000004	8
GTATGGT	20	6.952051E-4	44.000004	15
TTGTATG	20	6.952051E-4	44.000004	13
TTGTATA	20	6.952051E-4	44.000004	13
GAGTGGA	20	6.952051E-4	44.000004	20
CATCCCC	20	6.952051E-4	44.000004	7
ATAGGCT	40	5.315087E-9	44.000004	16
ACATCCC	20	6.952051E-4	44.000004	6
GTATAGT	20	6.952051E-4	44.000004	15
GGATAGG	45	2.728484E-10	44.000004	14
ATAGTTT	20	6.952051E-4	44.000004	17
CCCCGAC	20	6.952051E-4	44.000004	10
CCCGACT	20	6.952051E-4	44.000004	11
GACTGGA	20	6.952051E-4	44.000004	14
TGTATGG	20	6.952051E-4	44.000004	14
TGTATAG	20	6.952051E-4	44.000004	14
CCGACTG	20	6.952051E-4	44.000004	12
TATAGTT	20	6.952051E-4	44.000004	16
CGACTGG	20	6.952051E-4	44.000004	13
GATAGGC	45	2.728484E-10	44.000004	15
>>END_MODULE
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321337 spots for SRR5030907.sra
Written 321337 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
Read 321331 spots for SRR5030907.sra
Written 321331 spots for SRR5030907.sra
SRR ids: ['SRR5030907.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tchwe4na
SRR5030907.sra spots: 3855978
blocks: [[1, 321331], [321332, 642662], [642663, 963993], [963994, 1285324], [1285325, 1606655], [1606656, 1927986], [1927987, 2249317], [2249318, 2570648], [2570649, 2891979], [2891980, 3213310], [3213311, 3534641], [3534642, 3855978]]
SRR5030907 file size 667251
SRR5030907 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030907 SRR5030907_1.fastq
Input file:	SRR5030907_1.fastq
trimmed:	SRR5030907-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:41:49 2020 >> started

Wed Mar  4 06:41:51 2020 >> done (1.894s)
3855978 reads processed; of these:
    130 ( 0.00%) short reads filtered out after trimming by size control
      8 ( 0.00%) empty reads filtered out after trimming by size control
3855840 (100.00%) reads available; of these:
 221249 ( 5.74%) trimmed reads available after processing
3634591 (94.26%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     48	  0.00%
 19	     61	  0.00%
 20	    113	  0.00%
 21	    183	  0.00%
 22	    299	  0.01%
 23	    376	  0.01%
 24	    371	  0.01%
 25	    515	  0.01%
 26	    382	  0.01%
 27	    545	  0.01%
 28	    771	  0.02%
 29	   1124	  0.03%
 30	    824	  0.02%
 31	   1096	  0.03%
 32	   1012	  0.03%
 33	   1424	  0.04%
 34	   3864	  0.10%
 35	   3555	  0.09%
 36	   2469	  0.06%
 37	   2687	  0.07%
 38	   2998	  0.08%
 39	   3258	  0.08%
 40	   3589	  0.09%
 41	   3982	  0.10%
 42	   5384	  0.14%
 43	   6883	  0.18%
 44	  13325	  0.35%
 45	  15582	  0.40%
 46	  13493	  0.35%
 47	  19931	  0.52%
 48	  44838	  1.16%
 49	  66267	  1.72%
 50	3634591	 94.26%
3855840 reads passed initial QC


criterion=sequence-density
sequence-density=85.07
sequence-density-rank=1
fanout-score=17.91
fanout-score-rank=1
prefix-density=86.69
prefix-fanout=17.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC


criterion=fanout-score
sequence-density=85.07
sequence-density-rank=1
fanout-score=17.91
fanout-score-rank=1
prefix-density=86.69
prefix-fanout=17.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC -o SRR5030907 -
Input file:	STDIN
trimmed:	SRR5030907-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:42:06 2020 >> started

Wed Mar  4 06:42:10 2020 >> done (4.120s)
3766169 reads processed; of these:
 166323 ( 4.42%) short reads filtered out after trimming by size control
  11629 ( 0.31%) empty reads filtered out after trimming by size control
3588217 (95.27%) reads available; of these:
3283013 (91.49%) trimmed reads available after processing
 305204 ( 8.51%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  28565	  0.80%
 19	  42984	  1.20%
 20	 180698	  5.04%
 21	 412800	 11.50%
 22	1272498	 35.46%
 23	 572824	 15.96%
 24	 209408	  5.84%
 25	  37649	  1.05%
 26	  19359	  0.54%
 27	  17740	  0.49%
 28	  20004	  0.56%
 29	  16382	  0.46%
 30	  28676	  0.80%
 31	  34105	  0.95%
 32	  36100	  1.01%
 33	  62202	  1.73%
 34	  32103	  0.89%
 35	  22036	  0.61%
 36	  23501	  0.65%
 37	  14980	  0.42%
 38	  16210	  0.45%
 39	  16210	  0.45%
 40	  21686	  0.60%
 41	  28721	  0.80%
 42	  31008	  0.86%
 43	  23836	  0.66%
 44	  20582	  0.57%
 45	  24588	  0.69%
 46	  19288	  0.54%
 47	  15413	  0.43%
 48	   3309	  0.09%
 49	  12342	  0.34%
 50	 270410	  7.54%


criterion=sequence-density
sequence-density=8.61
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.45
sequence-density-rank=32
fanout-score=16.28
fanout-score-rank=1
prefix-density=7.29
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:42:30
                             Started mapping on |	Mar 04 06:42:31
                                    Finished on |	Mar 04 06:42:53
       Mapping speed, Million of reads per hour |	601.84

                          Number of input reads |	3677888
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1921098
                        Uniquely mapped reads % |	52.23%
                          Average mapped length |	26.15
                       Number of splices: Total |	1780
            Number of splices: Annotated (sjdb) |	227
                       Number of splices: GT/AG |	1609
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	151
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.53
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1407313
             % of reads mapped to multiple loci |	38.26%
        Number of reads mapped to too many loci |	72307
             % of reads mapped to too many loci |	1.97%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.92%
                     % of reads unmapped: other |	0.61%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	349477	349477	349477
N_multimapping	1407313	1407313	1407313
N_noFeature	83730	99631	1462615
N_ambiguous	1065757	676664	16039
UnstrandedReadsAssigned:771611 PositiveStrandReadsAssigned:1144803 NegativeStrandReadsAssigned:442444
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5030907 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030907-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,677,888 reads, 803,419 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 978 rounds

 109283 SRR5030907.ke.tsv
  52636 SRR5030907.se.tsv
 161919 total
==> SRR5030907.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030907.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030907 completed mapping pipeline successfully
