Starting /dee2/code/volunteer_pipeline.sh SRR5030888
    current disk space = 160643629056
    free memory = 73950144 
SRR5030888 SRAfilesize
43ed3cf466be315987a1b264ff71b81b  SRR5030888.sra
SRR5030888.sra file validated
SRR5030888 is single end
SRR5030888 is conventional basespace
SRR5030888 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030888_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34775	33.0	31.0	34.0	31.0	34.0
2	32.66225	34.0	31.0	34.0	31.0	34.0
3	32.7885	34.0	31.0	34.0	31.0	34.0
4	36.26225	37.0	37.0	37.0	35.0	37.0
5	36.29275	37.0	37.0	37.0	35.0	37.0
6	36.1115	37.0	36.0	37.0	35.0	37.0
7	36.07175	37.0	35.0	37.0	35.0	37.0
8	36.18725	37.0	36.0	37.0	35.0	37.0
9	37.92125	39.0	38.0	39.0	35.0	39.0
10	37.95675	39.0	38.0	39.0	35.0	39.0
11	37.82825	39.0	38.0	39.0	35.0	39.0
12	37.9275	39.0	38.0	39.0	35.0	39.0
13	37.8015	39.0	38.0	39.0	35.0	39.0
14	39.23	41.0	39.0	41.0	36.0	41.0
15	39.29975	41.0	39.0	41.0	36.0	41.0
16	39.25375	40.0	39.0	41.0	36.0	41.0
17	39.434	41.0	39.0	41.0	36.0	41.0
18	39.38225	41.0	39.0	41.0	36.0	41.0
19	39.18	41.0	39.0	41.0	36.0	41.0
20	39.07975	40.0	39.0	41.0	35.0	41.0
21	39.166	40.0	39.0	41.0	36.0	41.0
22	39.14225	41.0	39.0	41.0	36.0	41.0
23	39.10225	41.0	39.0	41.0	36.0	41.0
24	38.8075	40.0	39.0	41.0	35.0	41.0
25	39.0765	41.0	39.0	41.0	35.0	41.0
26	39.0645	41.0	39.0	41.0	36.0	41.0
27	39.20125	41.0	39.0	41.0	36.0	41.0
28	39.075	41.0	39.0	41.0	36.0	41.0
29	38.2685	40.0	38.0	41.0	33.0	41.0
30	38.48625	40.0	38.0	41.0	34.0	41.0
31	38.71775	40.0	38.0	41.0	34.0	41.0
32	38.857	40.0	39.0	41.0	35.0	41.0
33	37.87725	40.0	38.0	41.0	33.0	41.0
34	37.8295	40.0	38.0	41.0	32.0	41.0
35	37.52675	39.0	37.0	40.0	33.0	41.0
36	33.93775	38.0	30.0	40.0	23.0	41.0
37	35.71875	38.0	34.0	40.0	30.0	41.0
38	37.12675	39.0	37.0	40.0	32.0	41.0
39	36.969	39.0	37.0	40.0	31.0	41.0
40	37.05775	39.0	37.0	40.0	31.0	41.0
41	37.28375	39.0	37.0	41.0	32.0	41.0
42	37.45975	40.0	37.0	41.0	33.0	41.0
43	37.1615	40.0	37.0	41.0	32.0	41.0
44	37.08175	39.0	37.0	41.0	31.0	41.0
45	36.7675	39.0	36.0	41.0	31.0	41.0
46	35.00975	38.0	34.0	40.0	26.0	41.0
47	35.667	39.0	35.0	40.0	28.0	41.0
48	36.04875	39.0	35.0	40.0	30.0	41.0
49	35.16025	39.0	35.0	40.0	28.0	41.0
50	30.07625	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	1.0
17	0.0
18	3.0
19	3.0
20	2.0
21	3.0
22	9.0
23	6.0
24	14.0
25	16.0
26	20.0
27	25.0
28	30.0
29	44.0
30	45.0
31	51.0
32	79.0
33	118.0
34	160.0
35	201.0
36	316.0
37	527.0
38	1056.0
39	1267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.02004008016032	22.394789579158317	52.40480961923848	15.180360721442888
2	22.425	25.174999999999997	20.4	32.0
3	26.1	19.325	21.6	32.975
4	31.075000000000003	25.85	14.875	28.199999999999996
5	19.3	43.675000000000004	24.275	12.75
6	35.15	17.325	33.650000000000006	13.875000000000002
7	17.05	21.099999999999998	24.45	37.4
8	24.2	30.325000000000003	16.375	29.099999999999998
9	23.674999999999997	34.075	28.15	14.099999999999998
10	22.725	21.7	31.275	24.3
11	26.25	12.925	18.425	42.4
12	15.049999999999999	19.1	32.6	33.25
13	30.55	21.525	29.75	18.175
14	26.25	35.825	21.3	16.625
15	36.449999999999996	26.900000000000002	22.425	14.224999999999998
16	18.5	22.6	22.7	36.199999999999996
17	18.575	24.8	41.3	15.325
18	22.375	20.775	31.7	25.15
19	39.6	14.025000000000002	26.974999999999998	19.400000000000002
20	41.825	15.375	29.099999999999998	13.700000000000001
21	24.2	17.1	27.700000000000003	31.0
22	27.650000000000002	12.875	49.375	10.100000000000001
23	28.275	13.025	43.824999999999996	14.875
24	53.425	15.425	22.6	8.55
25	48.875	25.724999999999998	14.875	10.525
26	25.074999999999996	47.975	14.85	12.1
27	14.899999999999999	47.599999999999994	25.75	11.75
28	11.200000000000001	22.875	50.025	15.9
29	11.4	12.8	56.574999999999996	19.225
30	10.5	6.7250000000000005	34.725	48.05
31	15.525	6.375	47.349999999999994	30.75
32	29.25	6.9	19.0	44.85
33	55.974999999999994	8.975	12.975	22.075
34	60.875	8.325000000000001	16.8	14.000000000000002
35	63.775000000000006	7.3999999999999995	18.975	9.85
36	42.625	8.425	35.65	13.3
37	45.15	10.725	18.625	25.5
38	21.375	17.175	12.5	48.949999999999996
39	16.0	26.0	9.825000000000001	48.175000000000004
40	16.1	49.75	11.875	22.275
41	25.6	49.275000000000006	9.425	15.7
42	50.6	29.625	11.325000000000001	8.450000000000001
43	49.975	30.825000000000003	9.2	10.0
44	27.675	50.125	8.125	14.075
45	17.4	49.725	13.525	19.35
46	11.200000000000001	26.275	19.325	43.2
47	9.225	15.15	41.325	34.300000000000004
48	11.175	13.5	19.425	55.900000000000006
49	14.549999999999999	19.6	14.6	51.24999999999999
50	20.25	36.875	14.524999999999999	28.349999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	1.0
26	2.0
27	2.5
28	3.0
29	5.0
30	7.0
31	14.0
32	21.0
33	20.0
34	19.0
35	31.0
36	43.0
37	44.0
38	45.0
39	51.5
40	58.0
41	54.5
42	51.0
43	92.0
44	133.0
45	192.0
46	251.0
47	363.0
48	475.0
49	577.0
50	679.0
51	687.5
52	696.0
53	567.5
54	439.0
55	474.0
56	509.0
57	381.5
58	254.0
59	163.0
60	72.0
61	62.5
62	53.0
63	78.5
64	104.0
65	65.0
66	26.0
67	17.5
68	9.0
69	11.0
70	13.0
71	20.0
72	27.0
73	15.5
74	4.0
75	3.5
76	3.0
77	1.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.699999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00982800982801	31.75
2	7.923832923832924	6.45
3	3.992628992628992	4.875
4	2.211302211302211	3.5999999999999996
5	1.6584766584766584	3.375
6	1.167076167076167	2.85
7	0.4914004914004914	1.4000000000000001
8	0.4914004914004914	1.6
9	0.6142506142506142	2.25
>10	2.886977886977887	21.75
>50	0.36855036855036855	9.9
>100	0.18427518427518427	10.2
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	151	3.775	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	135	3.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	122	3.05	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	98	2.45	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	72	1.7999999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	67	1.675	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	54	1.35	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	53	1.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	52	1.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	47	1.175	RNA PCR Primer, Index 1 (100% over 27bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	38	0.95	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC	19	0.475	RNA PCR Primer, Index 3 (100% over 44bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT	18	0.44999999999999996	RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	15	0.375	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	13	0.325	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC	13	0.325	RNA PCR Primer, Index 3 (100% over 50bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	12	0.3	RNA PCR Primer, Index 1 (100% over 32bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	12	0.3	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	12	0.3	No Hit
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	11	0.27499999999999997	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	10	0.25	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	9	0.22499999999999998	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	9	0.22499999999999998	No Hit
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	9	0.22499999999999998	RNA PCR Primer, Index 3 (100% over 34bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	9	0.22499999999999998	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	8	0.2	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	8	0.2	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	8	0.2	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	8	0.2	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	6	0.15	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	6	0.15	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	6	0.15	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	6	0.15	No Hit
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTAT	6	0.15	RNA PCR Primer, Index 3 (100% over 48bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	5	0.125	RNA PCR Primer, Index 3 (100% over 34bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCCATGATGAGCAACACTCACCATCTTTCGTTTGAGTCTCACGACTGTG	5	0.125	No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GCTGTACTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTG	5	0.125	No Hit
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.325	0.0	0.0	0.0
2	0.0	0.325	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.525	0.0	0.0	0.0
5	0.0	0.55	0.0	0.0	0.0
6	0.0	0.575	0.0	0.0	0.0
7	0.0	1.2	0.0	0.0	0.0
8	0.0	1.35	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.45	0.0	0.0	0.0
11	0.0	1.625	0.0	0.0	0.0
12	0.0	1.8	0.0	0.0	0.0
13	0.0	2.05	0.0	0.0	0.0
14	0.0	2.325	0.0	0.0	0.0
15	0.0	2.675	0.0	0.0	0.0
16	0.0	3.625	0.0	0.0	0.0
17	0.0	4.425	0.0	0.0	0.0
18	0.0	5.375	0.0	0.0	0.0
19	0.0	6.125	0.0	0.0	0.0
20	0.0	7.075	0.0	0.0	0.0
21	0.0	13.15	0.0	0.0	0.0
22	0.0	24.975	0.0	0.0	0.0
23	0.0	55.55	0.0	0.0	0.0
24	0.0	66.575	0.0	0.0	0.0
25	0.0	72.125	0.0	0.0	0.0
26	0.0	73.175	0.0	0.0	0.0
27	0.0	73.725	0.0	0.0	0.0
28	0.0	74.325	0.0	0.0	0.0
29	0.0	74.7	0.0	0.0	0.0
30	0.0	75.175	0.0	0.0	0.0
31	0.0	75.725	0.0	0.0	0.0
32	0.0	76.35	0.0	0.0	0.0
33	0.0	77.175	0.0	0.0	0.0
34	0.0	80.05	0.0	0.0	0.0
35	0.0	80.85	0.0	0.0	0.0
36	0.0	81.5	0.0	0.0	0.0
37	0.0	82.275	0.0	0.0	0.0
38	0.0	82.725	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACAT	20	6.952051E-4	44.000004	3
AAACATC	20	6.952051E-4	44.000004	4
GAAGCTT	20	6.952051E-4	44.000004	19
GAGCTTG	40	5.315087E-9	44.000004	13
CTGGAAG	20	6.952051E-4	44.000004	16
GGCTTGG	20	6.952051E-4	44.000004	19
GGAAGCT	20	6.952051E-4	44.000004	18
GACTGGA	20	6.952051E-4	44.000004	14
TTGGCTT	20	6.952051E-4	44.000004	17
TGGAAGC	20	6.952051E-4	44.000004	17
ACTGGAA	20	6.952051E-4	44.000004	15
AAGCTTG	20	6.952051E-4	44.000004	20
TGAGCTT	40	5.315087E-9	44.000004	12
CTGTATG	20	6.952051E-4	44.000004	16
CGACTGG	20	6.952051E-4	44.000004	13
TGTAAAC	20	6.952051E-4	44.000004	1
GTAAACA	20	6.952051E-4	44.000004	2
AACATCC	20	6.952051E-4	44.000004	5
ACTGTTG	25	3.6958358E-5	44.0	18
AAGTAAT	35	1.02281774E-7	44.0	4
>>END_MODULE
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
Read 298051 spots for SRR5030888.sra
Written 298051 spots for SRR5030888.sra
Read 298050 spots for SRR5030888.sra
Written 298050 spots for SRR5030888.sra
SRR ids: ['SRR5030888.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_57bm4f8k
SRR5030888.sra spots: 3576601
blocks: [[1, 298050], [298051, 596100], [596101, 894150], [894151, 1192200], [1192201, 1490250], [1490251, 1788300], [1788301, 2086350], [2086351, 2384400], [2384401, 2682450], [2682451, 2980500], [2980501, 3278550], [3278551, 3576601]]
SRR5030888 file size 618829
SRR5030888 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030888 SRR5030888_1.fastq
Input file:	SRR5030888_1.fastq
trimmed:	SRR5030888-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:36:57 2020 >> started

Wed Mar  4 06:36:59 2020 >> done (1.974s)
3576601 reads processed; of these:
    131 ( 0.00%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
3576466 (100.00%) reads available; of these:
 215204 ( 6.02%) trimmed reads available after processing
3361262 (93.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     49	  0.00%
 19	     57	  0.00%
 20	    161	  0.00%
 21	    177	  0.00%
 22	    329	  0.01%
 23	    369	  0.01%
 24	    416	  0.01%
 25	    488	  0.01%
 26	    469	  0.01%
 27	    639	  0.02%
 28	    790	  0.02%
 29	   1122	  0.03%
 30	    887	  0.02%
 31	   1175	  0.03%
 32	   1132	  0.03%
 33	   1408	  0.04%
 34	   3279	  0.09%
 35	   2782	  0.08%
 36	   2206	  0.06%
 37	   2262	  0.06%
 38	   2683	  0.08%
 39	   2984	  0.08%
 40	   3291	  0.09%
 41	   3819	  0.11%
 42	   5385	  0.15%
 43	   7009	  0.20%
 44	  12743	  0.36%
 45	  15346	  0.43%
 46	  14402	  0.40%
 47	  20645	  0.58%
 48	  41511	  1.16%
 49	  65189	  1.82%
 50	3361262	 93.98%
3576466 reads passed initial QC


criterion=sequence-density
sequence-density=82.44
sequence-density-rank=1
fanout-score=19.02
fanout-score-rank=1
prefix-density=84.32
prefix-fanout=18.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC


criterion=fanout-score
sequence-density=82.44
sequence-density-rank=1
fanout-score=19.02
fanout-score-rank=1
prefix-density=84.32
prefix-fanout=18.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC -o SRR5030888 -
Input file:	STDIN
trimmed:	SRR5030888-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:37:16 2020 >> started

Wed Mar  4 06:37:22 2020 >> done (5.668s)
3490286 reads processed; of these:
 196960 ( 5.64%) short reads filtered out after trimming by size control
  12577 ( 0.36%) empty reads filtered out after trimming by size control
3280749 (94.00%) reads available; of these:
2940956 (89.64%) trimmed reads available after processing
 339793 (10.36%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  26727	  0.81%
 19	  37341	  1.14%
 20	 219665	  6.70%
 21	 439317	 13.39%
 22	1031627	 31.44%
 23	 396665	 12.09%
 24	 178225	  5.43%
 25	  42510	  1.30%
 26	  22950	  0.70%
 27	  20086	  0.61%
 28	  21764	  0.66%
 29	  16409	  0.50%
 30	  21327	  0.65%
 31	  23646	  0.72%
 32	  26113	  0.80%
 33	 108018	  3.29%
 34	  31831	  0.97%
 35	  24457	  0.75%
 36	  27042	  0.82%
 37	  14961	  0.46%
 38	  16049	  0.49%
 39	  16735	  0.51%
 40	  25173	  0.77%
 41	  30730	  0.94%
 42	  34514	  1.05%
 43	  25331	  0.77%
 44	  21151	  0.64%
 45	  24865	  0.76%
 46	  19922	  0.61%
 47	  17627	  0.54%
 48	   3634	  0.11%
 49	  12579	  0.38%
 50	 301758	  9.20%


criterion=sequence-density
sequence-density=4.30
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AAGCTGCCAGTTGAAGAACTGT


criterion=fanout-score
sequence-density=0.47
sequence-density-rank=24
fanout-score=16.92
fanout-score-rank=1
prefix-density=8.02
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:38:31
                             Started mapping on |	Mar 04 06:38:32
                                    Finished on |	Mar 04 06:38:57
       Mapping speed, Million of reads per hour |	484.84

                          Number of input reads |	3366929
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1919766
                        Uniquely mapped reads % |	57.02%
                          Average mapped length |	26.57
                       Number of splices: Total |	1432
            Number of splices: Annotated (sjdb) |	228
                       Number of splices: GT/AG |	1314
                       Number of splices: GC/AG |	17
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	99
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.57
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1047003
             % of reads mapped to multiple loci |	31.10%
        Number of reads mapped to too many loci |	70446
             % of reads mapped to too many loci |	2.09%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.33%
                     % of reads unmapped: other |	0.46%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	400160	400160	400160
N_multimapping	1047003	1047003	1047003
N_noFeature	78504	95977	1318847
N_ambiguous	1109780	587681	22067
UnstrandedReadsAssigned:731482 PositiveStrandReadsAssigned:1236108 NegativeStrandReadsAssigned:578852
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5030888 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030888-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,366,929 reads, 938,957 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,132 rounds

 109283 SRR5030888.ke.tsv
  52636 SRR5030888.se.tsv
 161919 total
==> SRR5030888.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030888.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	1
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030888 completed mapping pipeline successfully
