Starting /dee2/code/volunteer_pipeline.sh SRR5031000
    current disk space = 160560693248
    free memory = 73959548 
SRR5031000 SRAfilesize
c2aa5bf740323be80425f73466de10fe  SRR5031000.sra
SRR5031000.sra file validated
SRR5031000 is single end
SRR5031000 is conventional basespace
SRR5031000 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3215	33.0	31.0	34.0	31.0	34.0
2	32.51325	34.0	31.0	34.0	31.0	34.0
3	32.52925	34.0	31.0	34.0	31.0	34.0
4	36.197	37.0	35.0	37.0	35.0	37.0
5	36.13175	37.0	35.0	37.0	35.0	37.0
6	35.863	37.0	35.0	37.0	35.0	37.0
7	35.82425	37.0	35.0	37.0	35.0	37.0
8	35.9135	37.0	35.0	37.0	35.0	37.0
9	37.545	39.0	37.0	39.0	35.0	39.0
10	37.434	39.0	37.0	39.0	34.0	39.0
11	37.175	39.0	37.0	39.0	33.0	39.0
12	37.43425	39.0	37.0	39.0	34.0	39.0
13	37.49025	39.0	37.0	39.0	34.0	39.0
14	38.70975	40.0	38.0	41.0	34.0	41.0
15	38.68125	40.0	38.0	41.0	34.0	41.0
16	38.81875	40.0	38.0	41.0	35.0	41.0
17	39.01275	40.0	38.0	41.0	35.0	41.0
18	38.84825	40.0	38.0	41.0	35.0	41.0
19	38.75275	40.0	38.0	41.0	34.0	41.0
20	38.50525	40.0	38.0	41.0	34.0	41.0
21	38.71275	40.0	38.0	41.0	34.0	41.0
22	38.7285	40.0	38.0	41.0	34.0	41.0
23	38.34875	40.0	38.0	41.0	33.0	41.0
24	38.75525	40.0	38.0	41.0	34.0	41.0
25	38.80625	40.0	38.0	41.0	34.0	41.0
26	38.6775	40.0	38.0	41.0	35.0	41.0
27	38.8115	40.0	38.0	41.0	35.0	41.0
28	38.546	40.0	38.0	41.0	34.0	41.0
29	38.23775	40.0	38.0	41.0	33.0	41.0
30	38.20475	40.0	38.0	41.0	33.0	41.0
31	38.35425	40.0	38.0	41.0	34.0	41.0
32	38.258	40.0	38.0	41.0	33.0	41.0
33	37.695	40.0	37.0	41.0	33.0	41.0
34	37.67275	40.0	37.0	41.0	33.0	41.0
35	37.416	39.0	37.0	41.0	31.0	41.0
36	37.48075	39.0	37.0	41.0	31.0	41.0
37	36.86525	39.0	36.0	41.0	30.0	41.0
38	36.90925	39.0	35.0	40.0	30.0	41.0
39	37.51575	39.0	37.0	41.0	32.0	41.0
40	37.7155	40.0	37.0	41.0	33.0	41.0
41	37.7095	40.0	37.0	41.0	33.0	41.0
42	37.63275	40.0	37.0	41.0	32.0	41.0
43	37.6115	40.0	37.0	41.0	33.0	41.0
44	37.592	40.0	37.0	41.0	32.0	41.0
45	37.5895	40.0	37.0	41.0	33.0	41.0
46	37.304	40.0	36.0	41.0	32.0	41.0
47	36.59425	39.0	35.0	41.0	30.0	41.0
48	36.34375	39.0	35.0	41.0	30.0	41.0
49	36.45225	39.0	35.0	41.0	30.0	41.0
50	35.84725	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	3.0
19	0.0
20	5.0
21	5.0
22	6.0
23	5.0
24	7.0
25	14.0
26	14.0
27	23.0
28	19.0
29	45.0
30	61.0
31	62.0
32	94.0
33	145.0
34	177.0
35	207.0
36	281.0
37	460.0
38	746.0
39	1620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.45	23.075000000000003	49.775000000000006	17.7
2	26.64160401002506	25.263157894736842	18.170426065162907	29.924812030075188
3	24.975	19.525000000000002	23.775	31.724999999999998
4	35.675000000000004	26.825	14.099999999999998	23.400000000000002
5	23.025000000000002	43.375	23.625	9.975000000000001
6	33.825	15.5	32.175	18.5
7	15.575	19.950000000000003	23.549999999999997	40.925
8	24.025	26.125	18.45	31.4
9	20.200000000000003	31.2	29.4	19.2
10	20.974999999999998	25.45	27.375	26.200000000000003
11	32.375	9.925	17.424999999999997	40.275
12	16.525000000000002	22.075	28.375	33.025
13	31.75	18.2	32.025	18.025
14	30.049999999999997	29.65	20.549999999999997	19.75
15	34.425	27.500000000000004	23.549999999999997	14.524999999999999
16	21.125	19.5	21.025	38.35
17	22.425	24.3	39.324999999999996	13.950000000000001
18	24.5	18.6	27.825	29.075
19	38.95	13.325000000000001	28.000000000000004	19.725
20	39.375	16.6	30.425	13.600000000000001
21	26.075	19.925	26.674999999999997	27.325
22	31.374999999999996	14.35	44.25	10.025
23	26.375	13.025	40.925	19.675
24	51.849999999999994	13.100000000000001	24.85	10.2
25	47.375	26.875	15.475	10.274999999999999
26	27.825	44.775	13.3	14.099999999999998
27	17.7	44.074999999999996	22.1	16.125
28	12.425	24.15	45.525	17.9
29	15.6	12.174999999999999	52.175000000000004	20.05
30	9.775	6.925000000000001	36.55	46.75
31	13.625000000000002	5.175	46.35	34.849999999999994
32	30.95	6.175	21.15	41.725
33	51.349999999999994	12.0	13.075000000000001	23.575
34	59.25	7.925	17.424999999999997	15.4
35	62.5	7.9750000000000005	19.8	9.725
36	46.375	7.35	33.900000000000006	12.375
37	43.9	12.35	20.8	22.95
38	23.1	18.8	12.049999999999999	46.050000000000004
39	15.35	23.575	12.85	48.225
40	15.575	45.45	13.975000000000001	25.0
41	23.9	44.6	9.9	21.6
42	46.575	27.800000000000004	15.1	10.525
43	48.75	28.799999999999997	9.35	13.100000000000001
44	34.225	44.925	7.5	13.350000000000001
45	22.425	46.075	13.05	18.45
46	17.025000000000002	25.05	19.0	38.925
47	10.0	15.9	38.925	35.175
48	13.625000000000002	11.825	20.45	54.1
49	14.499999999999998	18.875	14.95	51.675000000000004
50	19.3	32.9	14.174999999999999	33.625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	2.5
28	4.0
29	3.0
30	2.0
31	9.0
32	16.0
33	15.0
34	14.0
35	15.5
36	17.0
37	20.5
38	24.0
39	30.5
40	37.0
41	38.0
42	39.0
43	77.5
44	116.0
45	145.5
46	175.0
47	279.0
48	383.0
49	515.5
50	648.0
51	715.0
52	782.0
53	592.0
54	402.0
55	467.0
56	532.0
57	389.0
58	246.0
59	168.5
60	91.0
61	69.5
62	48.0
63	152.5
64	257.0
65	152.0
66	47.0
67	32.5
68	18.0
69	23.5
70	29.0
71	42.0
72	55.0
73	31.0
74	7.0
75	3.5
76	0.0
77	1.5
78	3.0
79	3.0
80	3.0
81	2.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.25
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.25844245348036	27.3
2	9.441764300482426	6.8500000000000005
3	4.066161268090972	4.425
4	2.6878015161957274	3.9
5	1.7918676774638183	3.25
6	1.1716057891109581	2.55
7	0.7580978635423846	1.925
8	0.4824259131633356	1.4000000000000001
9	0.4824259131633356	1.575
>10	3.239145416953825	23.95
>50	0.34458993797381116	9.625
>100	0.2756719503790489	13.25
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	168	4.2	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	139	3.4750000000000005	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	104	2.6	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	95	2.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	89	2.225	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	85	2.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	54	1.35	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	47	1.175	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	38	0.95	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGC	38	0.95	RNA PCR Primer, Index 20 (100% over 50bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	32	0.8	RNA PCR Primer, Index 1 (97% over 35bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	31	0.775	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	30	0.75	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	28	0.7000000000000001	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	26	0.65	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	21	0.525	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	19	0.475	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC	15	0.375	RNA PCR Primer, Index 20 (100% over 44bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	14	0.35000000000000003	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	14	0.35000000000000003	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	13	0.325	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	11	0.27499999999999997	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	9	0.22499999999999998	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	9	0.22499999999999998	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	8	0.2	RNA PCR Primer, Index 6 (100% over 34bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	7	0.17500000000000002	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG	6	0.15	RNA PCR Primer, Index 17 (97% over 37bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTAT	6	0.15	RNA PCR Primer, Index 20 (100% over 48bp)
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	6	0.15	No Hit
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	6	0.15	RNA PCR Primer, Index 1 (97% over 35bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	5	0.125	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
ATCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.075	0.0	0.0	0.0
2	0.0	1.075	0.0	0.0	0.0
3	0.0	1.275	0.0	0.0	0.0
4	0.0	1.375	0.0	0.0	0.0
5	0.0	1.375	0.0	0.0	0.0
6	0.0	1.475	0.0	0.0	0.0
7	0.0	1.95	0.0	0.0	0.0
8	0.0	2.15	0.0	0.0	0.0
9	0.0	2.225	0.0	0.0	0.0
10	0.0	2.3	0.0	0.0	0.0
11	0.0	2.475	0.0	0.0	0.0
12	0.0	2.75	0.0	0.0	0.0
13	0.0	3.025	0.0	0.0	0.0
14	0.0	3.35	0.0	0.0	0.0
15	0.0	3.7	0.0	0.0	0.0
16	0.0	5.175	0.0	0.0	0.0
17	0.0	6.0	0.0	0.0	0.0
18	0.0	7.075	0.0	0.0	0.0
19	0.0	7.475	0.0	0.0	0.0
20	0.0	8.3	0.0	0.0	0.0
21	0.0	13.15	0.0	0.0	0.0
22	0.0	24.775	0.0	0.0	0.0
23	0.0	51.5	0.0	0.0	0.0
24	0.0	63.925	0.0	0.0	0.0
25	0.0	70.1	0.0	0.0	0.0
26	0.0	71.45	0.0	0.0	0.0
27	0.0	72.075	0.0	0.0	0.0
28	0.0	72.425	0.0	0.0	0.0
29	0.0	72.9	0.0	0.0	0.0
30	0.0	73.35	0.0	0.0	0.0
31	0.0	74.175	0.0	0.0	0.0
32	0.0	75.575	0.0	0.0	0.0
33	0.0	76.325	0.0	0.0	0.0
34	0.0	82.125	0.0	0.0	0.0
35	0.0	83.225	0.0	0.0	0.0
36	0.0	84.1	0.0	0.0	0.0
37	0.0	85.05	0.0	0.0	0.0
38	0.0	85.4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
AATCCAG	20	6.952051E-4	44.000004	8
TAAACAT	20	6.952051E-4	44.000004	3
AAACATC	20	6.952051E-4	44.000004	4
TTCAAGT	20	6.952051E-4	44.000004	1
ATGATGG	20	6.952051E-4	44.000004	20
ATAGGCT	20	6.952051E-4	44.000004	16
CTTGGTG	20	6.952051E-4	44.000004	16
AGTAATC	20	6.952051E-4	44.000004	5
AGGCTTG	20	6.952051E-4	44.000004	18
CTGGAAG	20	6.952051E-4	44.000004	16
TAATCCA	20	6.952051E-4	44.000004	7
GCTTGGT	20	6.952051E-4	44.000004	15
GACTGGA	20	6.952051E-4	44.000004	14
TTGGTGG	20	6.952051E-4	44.000004	17
TGGAAGC	20	6.952051E-4	44.000004	17
ACTGGAA	20	6.952051E-4	44.000004	15
GTAATCC	20	6.952051E-4	44.000004	6
TGTAAAC	20	6.952051E-4	44.000004	1
>>END_MODULE
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261444 spots for SRR5031000.sra
Written 261444 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
Read 261439 spots for SRR5031000.sra
Written 261439 spots for SRR5031000.sra
SRR ids: ['SRR5031000.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hmpdpgiz
SRR5031000.sra spots: 3137273
blocks: [[1, 261439], [261440, 522878], [522879, 784317], [784318, 1045756], [1045757, 1307195], [1307196, 1568634], [1568635, 1830073], [1830074, 2091512], [2091513, 2352951], [2352952, 2614390], [2614391, 2875829], [2875830, 3137273]]
SRR5031000 file size 542693
SRR5031000 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031000 SRR5031000_1.fastq
Input file:	SRR5031000_1.fastq
trimmed:	SRR5031000-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:03:36 2020 >> started

Wed Mar  4 07:03:38 2020 >> done (1.747s)
3137273 reads processed; of these:
    361 ( 0.01%) short reads filtered out after trimming by size control
     11 ( 0.00%) empty reads filtered out after trimming by size control
3136901 (99.99%) reads available; of these:
 210647 ( 6.72%) trimmed reads available after processing
2926254 (93.28%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     76	  0.00%
 19	     99	  0.00%
 20	    185	  0.01%
 21	    260	  0.01%
 22	    409	  0.01%
 23	    484	  0.02%
 24	    523	  0.02%
 25	    671	  0.02%
 26	    605	  0.02%
 27	    765	  0.02%
 28	    953	  0.03%
 29	   1274	  0.04%
 30	   1033	  0.03%
 31	   1167	  0.04%
 32	   1413	  0.05%
 33	   1525	  0.05%
 34	   1820	  0.06%
 35	   2973	  0.09%
 36	   2855	  0.09%
 37	   3243	  0.10%
 38	   3379	  0.11%
 39	   3755	  0.12%
 40	   4523	  0.14%
 41	   4387	  0.14%
 42	   5768	  0.18%
 43	   9021	  0.29%
 44	  12452	  0.40%
 45	  15740	  0.50%
 46	  22181	  0.71%
 47	  24337	  0.78%
 48	  36847	  1.17%
 49	  45924	  1.46%
 50	2926254	 93.28%
3136901 reads passed initial QC


criterion=sequence-density
sequence-density=85.77
sequence-density-rank=1
fanout-score=20.73
fanout-score-rank=1
prefix-density=87.34
prefix-fanout=20.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC


criterion=fanout-score
sequence-density=85.77
sequence-density-rank=1
fanout-score=20.73
fanout-score-rank=1
prefix-density=87.34
prefix-fanout=20.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC -o SRR5031000 -
Input file:	STDIN
trimmed:	SRR5031000-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:03:53 2020 >> started

Wed Mar  4 07:03:58 2020 >> done (4.290s)
3063950 reads processed; of these:
 173113 ( 5.65%) short reads filtered out after trimming by size control
  35259 ( 1.15%) empty reads filtered out after trimming by size control
2855578 (93.20%) reads available; of these:
2714078 (95.04%) trimmed reads available after processing
 141500 ( 4.96%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  14093	  0.49%
 19	  29652	  1.04%
 20	 153558	  5.38%
 21	 355470	 12.45%
 22	 852577	 29.86%
 23	 392929	 13.76%
 24	 190306	  6.66%
 25	  51774	  1.81%
 26	  25814	  0.90%
 27	  20032	  0.70%
 28	  20360	  0.71%
 29	  15731	  0.55%
 30	  25669	  0.90%
 31	  45933	  1.61%
 32	  31752	  1.11%
 33	 159184	  5.57%
 34	  34257	  1.20%
 35	  25475	  0.89%
 36	  32199	  1.13%
 37	  15426	  0.54%
 38	  17266	  0.60%
 39	  16324	  0.57%
 40	  23540	  0.82%
 41	  32402	  1.13%
 42	  40127	  1.41%
 43	  28090	  0.98%
 44	  22003	  0.77%
 45	  26248	  0.92%
 46	  19673	  0.69%
 47	  16229	  0.57%
 48	   1696	  0.06%
 49	   3025	  0.11%
 50	 116764	  4.09%


criterion=sequence-density
sequence-density=5.95
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=39
fanout-score=84.64
fanout-score-rank=1
prefix-density=1.72
prefix-fanout=1.0
sequence=ATTGTATAGTTTAACAGTCTACAGCCATGGTCGT
                                 Started job on |	Mar 04 07:05:01
                             Started mapping on |	Mar 04 07:05:02
                                    Finished on |	Mar 04 07:05:28
       Mapping speed, Million of reads per hour |	405.49

                          Number of input reads |	2928529
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1518163
                        Uniquely mapped reads % |	51.84%
                          Average mapped length |	24.69
                       Number of splices: Total |	1444
            Number of splices: Annotated (sjdb) |	321
                       Number of splices: GT/AG |	1346
                       Number of splices: GC/AG |	20
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	77
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.73
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	950407
             % of reads mapped to multiple loci |	32.45%
        Number of reads mapped to too many loci |	76095
             % of reads mapped to too many loci |	2.60%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.46%
                     % of reads unmapped: other |	0.65%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	459959	459959	459959
N_multimapping	950407	950407	950407
N_noFeature	94798	112529	1060065
N_ambiguous	816942	420090	14887
UnstrandedReadsAssigned:606423 PositiveStrandReadsAssigned:985544 NegativeStrandReadsAssigned:443211
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5031000 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031000-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,928,529 reads, 718,353 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 846 rounds

 109283 SRR5031000.ke.tsv
  52636 SRR5031000.se.tsv
 161919 total
==> SRR5031000.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031000.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031000 completed mapping pipeline successfully
