Starting /dee2/code/volunteer_pipeline.sh SRR5030963
    current disk space = 160570785792
    free memory = 73950496 
SRR5030963 SRAfilesize
3c4325d3940f190b9b548f3bd8ab1207  SRR5030963.sra
SRR5030963.sra file validated
SRR5030963 is single end
SRR5030963 is conventional basespace
SRR5030963 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4795	34.0	31.0	34.0	31.0	34.0
2	32.7255	34.0	31.0	34.0	31.0	34.0
3	32.86275	34.0	31.0	34.0	31.0	34.0
4	36.20925	37.0	37.0	37.0	35.0	37.0
5	36.28475	37.0	37.0	37.0	35.0	37.0
6	36.15825	37.0	37.0	37.0	35.0	37.0
7	36.0845	37.0	35.0	37.0	35.0	37.0
8	36.174	37.0	36.0	37.0	35.0	37.0
9	38.05275	39.0	38.0	39.0	35.0	39.0
10	38.0085	39.0	39.0	39.0	35.0	39.0
11	37.83025	39.0	38.0	39.0	35.0	39.0
12	37.88375	39.0	38.0	39.0	35.0	39.0
13	37.82825	39.0	38.0	39.0	35.0	39.0
14	39.26975	41.0	39.0	41.0	36.0	41.0
15	39.28675	41.0	39.0	41.0	36.0	41.0
16	39.24975	41.0	39.0	41.0	36.0	41.0
17	39.39825	41.0	39.0	41.0	36.0	41.0
18	39.34825	41.0	39.0	41.0	36.0	41.0
19	39.084	41.0	39.0	41.0	35.0	41.0
20	39.09975	40.0	39.0	41.0	36.0	41.0
21	39.1905	40.0	39.0	41.0	36.0	41.0
22	39.0655	41.0	39.0	41.0	36.0	41.0
23	38.959	40.0	39.0	41.0	35.0	41.0
24	38.74	40.0	38.0	41.0	35.0	41.0
25	39.024	41.0	39.0	41.0	35.0	41.0
26	39.0645	41.0	39.0	41.0	35.0	41.0
27	39.0325	41.0	39.0	41.0	35.0	41.0
28	38.788	40.0	38.0	41.0	35.0	41.0
29	38.16425	40.0	38.0	41.0	33.0	41.0
30	38.33625	40.0	38.0	41.0	34.0	41.0
31	38.6095	40.0	38.0	41.0	34.0	41.0
32	38.8575	40.0	38.0	41.0	35.0	41.0
33	37.9755	40.0	38.0	41.0	33.0	41.0
34	37.97575	40.0	38.0	41.0	33.0	41.0
35	37.94575	40.0	38.0	41.0	33.0	41.0
36	34.63625	38.0	33.0	40.0	23.0	41.0
37	35.728	38.0	35.0	40.0	29.0	41.0
38	37.129	39.0	37.0	40.0	32.0	41.0
39	37.0625	39.0	37.0	40.0	31.0	41.0
40	37.05475	39.0	37.0	40.0	31.0	41.0
41	37.1	39.0	37.0	41.0	31.0	41.0
42	37.21875	40.0	37.0	41.0	32.0	41.0
43	37.0505	40.0	37.0	41.0	31.0	41.0
44	36.90375	39.0	36.0	41.0	31.0	41.0
45	36.633	39.0	36.0	40.0	31.0	41.0
46	34.99825	38.0	34.0	40.0	26.0	41.0
47	35.39875	38.0	34.0	40.0	28.0	41.0
48	35.50775	39.0	35.0	40.0	28.0	41.0
49	34.60725	38.0	34.0	40.0	26.0	41.0
50	29.9315	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	1.0
17	1.0
18	0.0
19	2.0
20	6.0
21	6.0
22	7.0
23	11.0
24	15.0
25	14.0
26	9.0
27	34.0
28	39.0
29	31.0
30	43.0
31	51.0
32	83.0
33	132.0
34	164.0
35	181.0
36	336.0
37	539.0
38	993.0
39	1298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.539424280350438	23.67959949937422	50.463078848560706	14.317897371714643
2	21.025	25.624999999999996	19.925	33.425
3	26.224999999999998	16.5	22.575	34.699999999999996
4	33.25	28.15	13.875000000000002	24.725
5	20.150000000000002	40.525	23.825	15.5
6	29.625	17.150000000000002	39.725	13.5
7	17.375	20.474999999999998	24.325	37.824999999999996
8	23.525	30.275000000000002	15.174999999999999	31.025000000000002
9	26.8	32.625	26.8	13.775
10	25.1	18.2	32.925	23.775
11	28.4	16.875	14.7	40.025
12	14.7	19.950000000000003	33.1	32.25
13	27.950000000000003	19.425	29.799999999999997	22.825
14	26.325	34.275	20.575	18.825
15	35.425000000000004	24.099999999999998	25.25	15.225
16	16.375	24.15	20.575	38.9
17	22.675	22.325	39.4	15.6
18	22.35	17.125	37.075	23.45
19	37.425000000000004	15.225	29.349999999999998	18.0
20	43.2	16.475	24.474999999999998	15.85
21	23.150000000000002	19.825	26.474999999999998	30.55
22	27.950000000000003	15.675	44.525	11.85
23	28.775000000000002	13.900000000000002	40.925	16.400000000000002
24	48.65	17.549999999999997	24.175	9.625
25	45.324999999999996	26.075	15.525	13.075000000000001
26	25.95	44.824999999999996	17.0	12.225
27	16.900000000000002	44.0	26.424999999999997	12.675
28	12.825000000000001	24.65	46.400000000000006	16.125
29	10.625	15.65	54.675	19.05
30	11.450000000000001	8.799999999999999	33.900000000000006	45.85
31	16.0	8.0	44.55	31.45
32	28.9	8.35	20.674999999999997	42.075
33	53.849999999999994	10.299999999999999	14.224999999999998	21.625
34	56.45	8.975	18.375	16.2
35	60.75000000000001	8.325000000000001	18.75	12.174999999999999
36	40.975	9.775	33.800000000000004	15.45
37	43.75	11.625	18.2	26.424999999999997
38	22.475	18.05	12.9	46.575
39	17.075000000000003	26.55	9.700000000000001	46.675
40	17.25	45.875	12.475	24.4
41	27.3	45.15	10.725	16.825000000000003
42	48.3	28.925	12.174999999999999	10.6
43	46.975	32.800000000000004	9.75	10.475
44	28.050000000000004	47.075	8.6	16.275000000000002
45	19.375	48.175000000000004	13.225000000000001	19.225
46	12.2	27.85	19.75	40.2
47	10.525	16.675	38.35	34.449999999999996
48	11.275	15.25	19.925	53.55
49	16.075	20.349999999999998	15.174999999999999	48.4
50	20.674999999999997	35.375	14.575	29.375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.0
21	0.5
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	2.5
30	3.0
31	12.0
32	21.0
33	23.5
34	26.0
35	43.5
36	61.0
37	70.5
38	80.0
39	75.5
40	71.0
41	75.0
42	79.0
43	100.0
44	121.0
45	164.5
46	208.0
47	291.0
48	374.0
49	458.5
50	543.0
51	660.5
52	778.0
53	585.0
54	392.0
55	484.0
56	576.0
57	455.0
58	334.0
59	222.0
60	110.0
61	74.0
62	38.0
63	56.0
64	74.0
65	53.0
66	32.0
67	24.0
68	16.0
69	12.0
70	8.0
71	18.0
72	28.0
73	18.0
74	8.0
75	4.5
76	1.0
77	1.0
78	1.0
79	5.5
80	10.0
81	5.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.55
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63176895306859	31.424999999999997
2	10.22864019253911	8.5
3	3.790613718411552	4.725
4	2.166064981949458	3.5999999999999996
5	1.3237063778580023	2.75
6	1.3237063778580023	3.3000000000000003
7	0.9626955475330927	2.8000000000000003
8	0.48134777376654636	1.6
9	0.421179302045728	1.575
>10	3.1889290012033693	21.6
>50	0.36101083032490977	11.15
>100	0.12033694344163659	6.9750000000000005
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	163	4.075	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	116	2.9000000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	94	2.35	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	77	1.925	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	76	1.9	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	73	1.825	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	69	1.725	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	57	1.425	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	30	0.75	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	30	0.75	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTC	28	0.7000000000000001	RNA PCR Primer, Index 22 (100% over 44bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	27	0.675	RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	27	0.675	RNA PCR Primer, Index 2 (100% over 35bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	18	0.44999999999999996	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	16	0.4	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	14	0.35000000000000003	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	12	0.3	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	11	0.27499999999999997	No Hit
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	11	0.27499999999999997	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	11	0.27499999999999997	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGC	11	0.27499999999999997	RNA PCR Primer, Index 22 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	10	0.25	RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	10	0.25	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	9	0.22499999999999998	No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	8	0.2	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	8	0.2	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	8	0.2	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	8	0.2	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	7	0.17500000000000002	No Hit
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	7	0.17500000000000002	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	7	0.17500000000000002	No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAAT	6	0.15	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	6	0.15	No Hit
TCTGGCTCCGTGTCTTCACTCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
GGTCAATGATGACAACCCAATGTCATGAACAAAGGTGATGACATAAAATT	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	5	0.125	No Hit
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATCATCGTCTCAAATGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	5	0.125	RNA PCR Primer, Index 2 (100% over 35bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGA	5	0.125	RNA PCR Primer, Index 22 (100% over 40bp)
GATACCCGTCGCCGCGCTCTCCCTCTCCCCCCGTCCGCCTCCCGGGCGGG	5	0.125	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.3	0.0	0.0	0.0
2	0.0	0.325	0.0	0.0	0.0
3	0.0	0.4	0.0	0.0	0.0
4	0.0	0.425	0.0	0.0	0.0
5	0.0	0.5	0.0	0.0	0.0
6	0.0	0.55	0.0	0.0	0.0
7	0.0	1.5	0.0	0.0	0.0
8	0.0	1.725	0.0	0.0	0.0
9	0.0	1.8	0.0	0.0	0.0
10	0.0	1.9	0.0	0.0	0.0
11	0.0	2.175	0.0	0.0	0.0
12	0.0	2.45	0.0	0.0	0.0
13	0.0	2.725	0.0	0.0	0.0
14	0.0	3.1	0.0	0.0	0.0
15	0.0	3.575	0.0	0.0	0.0
16	0.0	4.75	0.0	0.0	0.0
17	0.0	5.325	0.0	0.0	0.0
18	0.0	6.35	0.0	0.0	0.0
19	0.0	7.325	0.0	0.0	0.0
20	0.0	8.15	0.0	0.0	0.0
21	0.0	14.7	0.0	0.0	0.0
22	0.0	25.975	0.0	0.0	0.0
23	0.0	52.95	0.0	0.0	0.0
24	0.0	63.475	0.0	0.0	0.0
25	0.0	69.025	0.0	0.0	0.0
26	0.0	70.45	0.0	0.0	0.0
27	0.0	71.25	0.0	0.0	0.0
28	0.0	71.925	0.0	0.0	0.0
29	0.0	72.55	0.0	0.0	0.0
30	0.0	72.9	0.0	0.0	0.0
31	0.0	73.575	0.0	0.0	0.0
32	0.0	74.3	0.0	0.0	0.0
33	0.0	75.15	0.0	0.0	0.0
34	0.0	77.1	0.0	0.0	0.0
35	0.0	78.075	0.0	0.0	0.0
36	0.0	78.975	0.0	0.0	0.0
37	0.0	79.425	0.0	0.0	0.0
38	0.0	79.9	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
GGTGAGT	20	6.952051E-4	44.000004	17
AAGTAAT	45	2.728484E-10	44.000004	4
TCAAGTA	45	2.728484E-10	44.000004	2
ACGCTGT	20	6.952051E-4	44.000004	9
AATCCAG	40	5.315087E-9	44.000004	8
ATCCAGG	40	5.315087E-9	44.000004	9
TTCAAGT	45	2.728484E-10	44.000004	1
TTCAACG	20	6.952051E-4	44.000004	5
AGGATAG	45	2.728484E-10	44.000004	13
TCGGTGA	20	6.952051E-4	44.000004	15
ATAGGCT	45	2.728484E-10	44.000004	16
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
TCGGATC	45	2.728484E-10	44.000004	1
CATTCAA	20	6.952051E-4	44.000004	3
TCAACGC	20	6.952051E-4	44.000004	6
CGGATCC	45	2.728484E-10	44.000004	2
TCCAGGA	40	5.315087E-9	44.000004	10
>>END_MODULE
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226536 spots for SRR5030963.sra
Written 226536 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
Read 226529 spots for SRR5030963.sra
Written 226529 spots for SRR5030963.sra
SRR ids: ['SRR5030963.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_b09zn084
SRR5030963.sra spots: 2718355
blocks: [[1, 226529], [226530, 453058], [453059, 679587], [679588, 906116], [906117, 1132645], [1132646, 1359174], [1359175, 1585703], [1585704, 1812232], [1812233, 2038761], [2038762, 2265290], [2265291, 2491819], [2491820, 2718355]]
SRR5030963 file size 470072
SRR5030963 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030963 SRR5030963_1.fastq
Input file:	SRR5030963_1.fastq
trimmed:	SRR5030963-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:55:17 2020 >> started

Wed Mar  4 06:55:19 2020 >> done (1.788s)
2718355 reads processed; of these:
    148 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2718201 (99.99%) reads available; of these:
 177087 ( 6.51%) trimmed reads available after processing
2541114 (93.49%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     42	  0.00%
 19	     60	  0.00%
 20	    128	  0.00%
 21	    118	  0.00%
 22	    242	  0.01%
 23	    283	  0.01%
 24	    343	  0.01%
 25	    460	  0.02%
 26	    355	  0.01%
 27	    488	  0.02%
 28	    653	  0.02%
 29	    914	  0.03%
 30	    680	  0.03%
 31	    832	  0.03%
 32	    814	  0.03%
 33	   1261	  0.05%
 34	   2253	  0.08%
 35	   2321	  0.09%
 36	   1776	  0.07%
 37	   1980	  0.07%
 38	   2203	  0.08%
 39	   2711	  0.10%
 40	   3031	  0.11%
 41	   3257	  0.12%
 42	   4144	  0.15%
 43	   5431	  0.20%
 44	   9905	  0.36%
 45	  12933	  0.48%
 46	  12782	  0.47%
 47	  19440	  0.72%
 48	  34944	  1.29%
 49	  50303	  1.85%
 50	2541114	 93.49%
2718201 reads passed initial QC


criterion=sequence-density
sequence-density=79.43
sequence-density-rank=1
fanout-score=18.19
fanout-score-rank=1
prefix-density=81.39
prefix-fanout=17.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC


criterion=fanout-score
sequence-density=79.43
sequence-density-rank=1
fanout-score=18.19
fanout-score-rank=1
prefix-density=81.39
prefix-fanout=17.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC -o SRR5030963 -
Input file:	STDIN
trimmed:	SRR5030963-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGTACGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:55:33 2020 >> started

Wed Mar  4 06:55:37 2020 >> done (3.466s)
2650246 reads processed; of these:
 169849 ( 6.41%) short reads filtered out after trimming by size control
  10290 ( 0.39%) empty reads filtered out after trimming by size control
2470107 (93.20%) reads available; of these:
2170727 (87.88%) trimmed reads available after processing
 299380 (12.12%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  19585	  0.79%
 19	  21751	  0.88%
 20	 160724	  6.51%
 21	 288221	 11.67%
 22	 742103	 30.04%
 23	 287160	 11.63%
 24	 151937	  6.15%
 25	  42608	  1.72%
 26	  20518	  0.83%
 27	  15720	  0.64%
 28	  17898	  0.72%
 29	  13366	  0.54%
 30	  17813	  0.72%
 31	  22821	  0.92%
 32	  23624	  0.96%
 33	  54565	  2.21%
 34	  21052	  0.85%
 35	  16824	  0.68%
 36	  16482	  0.67%
 37	  13743	  0.56%
 38	  14358	  0.58%
 39	  15609	  0.63%
 40	  24804	  1.00%
 41	  30060	  1.22%
 42	  34101	  1.38%
 43	  24370	  0.99%
 44	  19538	  0.79%
 45	  25828	  1.05%
 46	  19269	  0.78%
 47	  14162	  0.57%
 48	   3263	  0.13%
 49	  11589	  0.47%
 50	 264641	 10.71%


criterion=sequence-density
sequence-density=4.83
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.47
sequence-density-rank=29
fanout-score=21.70
fanout-score-rank=1
prefix-density=10.25
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:55:56
                             Started mapping on |	Mar 04 06:55:57
                                    Finished on |	Mar 04 06:56:20
       Mapping speed, Million of reads per hour |	397.26

                          Number of input reads |	2538062
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1590004
                        Uniquely mapped reads % |	62.65%
                          Average mapped length |	27.40
                       Number of splices: Total |	1083
            Number of splices: Annotated (sjdb) |	176
                       Number of splices: GT/AG |	984
                       Number of splices: GC/AG |	20
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	79
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.61
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	667600
             % of reads mapped to multiple loci |	26.30%
        Number of reads mapped to too many loci |	49413
             % of reads mapped to too many loci |	1.95%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.18%
                     % of reads unmapped: other |	0.92%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	280458	280458	280458
N_multimapping	667600	667600	667600
N_noFeature	59684	78764	1055561
N_ambiguous	899353	427894	14534
UnstrandedReadsAssigned:630967 PositiveStrandReadsAssigned:1083346 NegativeStrandReadsAssigned:519909
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030963 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030963-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,538,062 reads, 727,214 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,082 rounds

 109283 SRR5030963.ke.tsv
  52636 SRR5030963.se.tsv
 161919 total
==> SRR5030963.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030963.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030963 completed mapping pipeline successfully
