Starting /dee2/code/volunteer_pipeline.sh SRR5030915
    current disk space = 160637812736
    free memory = 73944416 
SRR5030915 SRAfilesize
ecf8c49928453c9bafa0a4cec4cc39b4  SRR5030915.sra
SRR5030915.sra file validated
SRR5030915 is single end
SRR5030915 is conventional basespace
SRR5030915 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52225	34.0	31.0	34.0	31.0	34.0
2	32.741	34.0	31.0	34.0	31.0	34.0
3	32.84625	34.0	31.0	34.0	31.0	34.0
4	36.244	37.0	37.0	37.0	35.0	37.0
5	36.3505	37.0	37.0	37.0	35.0	37.0
6	36.124	37.0	36.0	37.0	35.0	37.0
7	36.0695	37.0	35.0	37.0	35.0	37.0
8	36.15525	37.0	36.0	37.0	35.0	37.0
9	37.9695	39.0	38.0	39.0	35.0	39.0
10	37.9745	39.0	38.0	39.0	35.0	39.0
11	37.8595	39.0	38.0	39.0	35.0	39.0
12	37.93975	39.0	38.0	39.0	35.0	39.0
13	37.80225	39.0	38.0	39.0	35.0	39.0
14	39.339	41.0	39.0	41.0	36.0	41.0
15	39.29875	41.0	39.0	41.0	36.0	41.0
16	39.24875	40.0	39.0	41.0	36.0	41.0
17	39.469	41.0	39.0	41.0	36.0	41.0
18	39.33375	41.0	39.0	41.0	36.0	41.0
19	39.05425	40.0	39.0	41.0	36.0	41.0
20	39.03825	40.0	39.0	41.0	36.0	41.0
21	39.17825	40.0	39.0	41.0	36.0	41.0
22	39.184	41.0	39.0	41.0	36.0	41.0
23	38.945	40.0	39.0	41.0	35.0	41.0
24	38.82075	40.0	38.0	41.0	35.0	41.0
25	39.1345	41.0	39.0	41.0	36.0	41.0
26	39.101	41.0	39.0	41.0	36.0	41.0
27	39.0875	41.0	39.0	41.0	36.0	41.0
28	38.93375	41.0	39.0	41.0	35.0	41.0
29	38.176	40.0	38.0	41.0	33.0	41.0
30	38.59525	40.0	38.0	41.0	34.0	41.0
31	38.7535	40.0	39.0	41.0	34.0	41.0
32	39.008	40.0	39.0	41.0	35.0	41.0
33	38.23875	40.0	38.0	41.0	34.0	41.0
34	38.28225	40.0	38.0	41.0	33.0	41.0
35	37.931	40.0	38.0	41.0	33.0	41.0
36	34.5315	38.0	32.0	40.0	23.0	41.0
37	35.911	38.0	35.0	40.0	30.0	41.0
38	37.162	39.0	37.0	40.0	32.0	41.0
39	37.1815	39.0	37.0	40.0	32.0	41.0
40	37.2335	39.0	37.0	41.0	32.0	41.0
41	37.37525	40.0	37.0	41.0	33.0	41.0
42	37.463	40.0	37.0	41.0	33.0	41.0
43	37.28175	40.0	37.0	41.0	32.0	41.0
44	37.0795	40.0	37.0	41.0	31.0	41.0
45	36.83475	40.0	37.0	41.0	31.0	41.0
46	35.221	38.0	34.0	40.0	26.0	41.0
47	35.98375	39.0	35.0	40.0	30.0	41.0
48	36.189	39.0	36.0	41.0	30.0	41.0
49	35.41775	39.0	35.0	40.0	28.0	41.0
50	30.779	34.0	28.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	0.0
15	0.0
16	2.0
17	2.0
18	0.0
19	0.0
20	10.0
21	6.0
22	2.0
23	13.0
24	9.0
25	8.0
26	25.0
27	22.0
28	26.0
29	43.0
30	38.0
31	55.0
32	70.0
33	107.0
34	142.0
35	216.0
36	270.0
37	531.0
38	1034.0
39	1365.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.725	23.3	49.525000000000006	16.45
2	23.525	28.65	20.9	26.924999999999997
3	18.5	18.475	21.175	41.85
4	26.125	35.775	13.875000000000002	24.224999999999998
5	20.65	37.85	27.775	13.725000000000001
6	31.974999999999998	16.650000000000002	39.050000000000004	12.325
7	16.275000000000002	22.125	24.9	36.7
8	23.025000000000002	41.4	14.149999999999999	21.425
9	20.9	36.1	29.775000000000002	13.225000000000001
10	22.925	18.95	25.8	32.324999999999996
11	32.275	15.625	13.775	38.324999999999996
12	16.7	18.4	24.15	40.75
13	21.725	19.7	35.675000000000004	22.900000000000002
14	37.475	23.7	24.3	14.524999999999999
15	35.35	23.974999999999998	28.999999999999996	11.675
16	14.325	25.275	21.3	39.1
17	26.400000000000002	21.75	36.199999999999996	15.65
18	29.375	16.950000000000003	30.625000000000004	23.05
19	36.425000000000004	14.149999999999999	32.45	16.975
20	48.15	18.4	21.65	11.799999999999999
21	21.224999999999998	28.4	22.0	28.375
22	33.525	13.750000000000002	43.0	9.725
23	24.0	13.65	46.2	16.150000000000002
24	48.975	13.55	28.95	8.525
25	51.925	21.75	14.6	11.725
26	30.4	44.2	14.424999999999999	10.975
27	14.725	49.05	21.875	14.35
28	11.899999999999999	27.025	47.349999999999994	13.725000000000001
29	10.05	12.975	58.3	18.675
30	10.95	6.875000000000001	37.775	44.4
31	14.149999999999999	5.425	45.025	35.4
32	26.125	6.75	23.825	43.3
33	51.37500000000001	7.85	14.524999999999999	26.25
34	62.025	7.3	15.55	15.125
35	63.87500000000001	8.975	17.549999999999997	9.6
36	43.974999999999994	9.625	34.300000000000004	12.1
37	42.8	11.175	23.599999999999998	22.425
38	24.875	14.899999999999999	13.900000000000002	46.325
39	15.75	23.025000000000002	9.4	51.824999999999996
40	14.399999999999999	46.425	12.1	27.075
41	22.8	50.7	10.125	16.375
42	48.949999999999996	31.275	10.375	9.4
43	52.87500000000001	28.299999999999997	8.975	9.85
44	32.324999999999996	46.175	8.774999999999999	12.725
45	17.1	53.175	12.049999999999999	17.675
46	12.425	29.575000000000003	18.75	39.25
47	8.55	15.299999999999999	39.15	37.0
48	11.15	11.200000000000001	23.674999999999997	53.974999999999994
49	13.425	18.95	15.475	52.15
50	18.8	36.25	12.2	32.75
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.0
26	1.0
27	1.0
28	1.0
29	1.5
30	2.0
31	11.0
32	20.0
33	22.0
34	24.0
35	38.5
36	53.0
37	56.5
38	60.0
39	55.5
40	51.0
41	57.5
42	64.0
43	87.5
44	111.0
45	151.0
46	191.0
47	343.5
48	496.0
49	534.0
50	572.0
51	761.5
52	951.0
53	672.5
54	394.0
55	389.5
56	385.0
57	351.0
58	317.0
59	191.5
60	66.0
61	51.5
62	37.0
63	62.0
64	87.0
65	55.0
66	23.0
67	17.5
68	12.0
69	14.0
70	16.0
71	33.0
72	50.0
73	28.0
74	6.0
75	4.0
76	2.0
77	1.5
78	1.0
79	1.5
80	2.0
81	2.0
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23552374756018	28.525
2	9.694209499024073	7.449999999999999
3	3.903708523096942	4.5
4	3.383214053350683	5.2
5	1.951854261548471	3.75
6	1.1060507482108002	2.55
7	1.040988939492518	2.8000000000000003
8	0.6506180871828237	2.0
9	0.520494469746259	1.7999999999999998
>10	3.188028627195836	25.575
>50	0.13012361743656475	4.275
>100	0.1951854261548471	11.575000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	172	4.3	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	172	4.3	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	119	2.9749999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	97	2.4250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	47	1.175	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	40	1.0	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	32	0.8	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	31	0.775	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	27	0.675	RNA PCR Primer, Index 1 (100% over 33bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA	22	0.5499999999999999	RNA PCR Primer, Index 7 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (96% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	13	0.325	No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC	12	0.3	RNA PCR Primer, Index 10 (100% over 50bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	11	0.27499999999999997	No Hit
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	10	0.25	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC	9	0.22499999999999998	RNA PCR Primer, Index 10 (100% over 44bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	9	0.22499999999999998	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	9	0.22499999999999998	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	9	0.22499999999999998	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCCAGGAACTCCAGTCACTA	8	0.2	RNA PCR Primer, Index 10 (97% over 35bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	8	0.2	No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	7	0.17500000000000002	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGCATGATGACTTGAATTGTCGGATACCCCTTCACCCCGTTCATGGGT	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	7	0.17500000000000002	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	6	0.15	No Hit
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	6	0.15	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGACACTCTGTGCTGAATGTC	6	0.15	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	5	0.125	No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTC	5	0.125	No Hit
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	5	0.125	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	5	0.125	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATAGTGGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTC	5	0.125	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.3	0.0	0.0	0.0
2	0.0	0.3	0.0	0.0	0.0
3	0.0	0.325	0.0	0.0	0.0
4	0.0	0.325	0.0	0.0	0.0
5	0.0	0.4	0.0	0.0	0.0
6	0.0	0.45	0.0	0.0	0.0
7	0.0	0.775	0.0	0.0	0.0
8	0.0	0.875	0.0	0.0	0.0
9	0.0	0.875	0.0	0.0	0.0
10	0.0	0.9	0.0	0.0	0.0
11	0.0	1.075	0.0	0.0	0.0
12	0.0	1.35	0.0	0.0	0.0
13	0.0	1.55	0.0	0.0	0.0
14	0.0	1.85	0.0	0.0	0.0
15	0.0	2.25	0.0	0.0	0.0
16	0.0	3.4	0.0	0.0	0.0
17	0.0	3.925	0.0	0.0	0.0
18	0.0	5.3	0.0	0.0	0.0
19	0.0	5.725	0.0	0.0	0.0
20	0.0	6.9	0.0	0.0	0.0
21	0.0	11.3	0.0	0.0	0.0
22	0.0	21.4	0.0	0.0	0.0
23	0.0	49.95	0.0	0.0	0.0
24	0.0	65.15	0.0	0.0	0.0
25	0.0	70.625	0.0	0.0	0.0
26	0.0	71.6	0.0	0.0	0.0
27	0.0	72.0	0.0	0.0	0.0
28	0.0	72.325	0.0	0.0	0.0
29	0.0	72.8	0.0	0.0	0.0
30	0.0	73.1	0.0	0.0	0.0
31	0.0	73.775	0.0	0.0	0.0
32	0.0	74.975	0.0	0.0	0.0
33	0.0	75.85	0.0	0.0	0.0
34	0.0	78.05	0.0	0.0	0.0
35	0.0	79.325	0.0	0.0	0.0
36	0.0	79.95	0.0	0.0	0.0
37	0.0	80.625	0.0	0.0	0.0
38	0.0	81.1	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.952051E-4	44.000004	2
GGTGAGT	40	5.315087E-9	44.000004	17
TTGTATA	20	6.952051E-4	44.000004	13
GAGTGGA	20	6.952051E-4	44.000004	20
AGTGGAA	20	6.952051E-4	44.000004	21
GTGAGTG	20	6.952051E-4	44.000004	18
GAGTTGG	20	6.952051E-4	44.000004	20
GTATAGT	20	6.952051E-4	44.000004	15
AGGCTTG	20	6.952051E-4	44.000004	18
CGGTGAG	45	2.728484E-10	44.000004	16
GGTAGTA	20	6.952051E-4	44.000004	4
TGAGGTA	20	6.952051E-4	44.000004	1
TGTATAG	20	6.952051E-4	44.000004	14
TGAGTTG	20	6.952051E-4	44.000004	19
TATAGTT	20	6.952051E-4	44.000004	16
TGAGTGG	20	6.952051E-4	44.000004	19
GTAGTAG	20	6.952051E-4	44.000004	5
AACGCTG	55	0.0	44.0	8
AAGTAAT	30	1.9515464E-6	44.0	4
TCAAGTA	30	1.9515464E-6	44.0	2
>>END_MODULE
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269429 spots for SRR5030915.sra
Written 269429 spots for SRR5030915.sra
Read 269430 spots for SRR5030915.sra
Written 269430 spots for SRR5030915.sra
SRR ids: ['SRR5030915.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_sko5ss_g
SRR5030915.sra spots: 3233149
blocks: [[1, 269429], [269430, 538858], [538859, 808287], [808288, 1077716], [1077717, 1347145], [1347146, 1616574], [1616575, 1886003], [1886004, 2155432], [2155433, 2424861], [2424862, 2694290], [2694291, 2963719], [2963720, 3233149]]
SRR5030915 file size 559301
SRR5030915 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030915 SRR5030915_1.fastq
Input file:	SRR5030915_1.fastq
trimmed:	SRR5030915-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:43:41 2020 >> started

Wed Mar  4 06:43:42 2020 >> done (1.866s)
3233149 reads processed; of these:
    129 ( 0.00%) short reads filtered out after trimming by size control
      7 ( 0.00%) empty reads filtered out after trimming by size control
3233013 (100.00%) reads available; of these:
 177412 ( 5.49%) trimmed reads available after processing
3055601 (94.51%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     26	  0.00%
 19	     58	  0.00%
 20	    107	  0.00%
 21	    147	  0.00%
 22	    276	  0.01%
 23	    312	  0.01%
 24	    339	  0.01%
 25	    412	  0.01%
 26	    351	  0.01%
 27	    487	  0.02%
 28	    656	  0.02%
 29	   1111	  0.03%
 30	    716	  0.02%
 31	    902	  0.03%
 32	    813	  0.03%
 33	   1137	  0.04%
 34	   2979	  0.09%
 35	   2729	  0.08%
 36	   2096	  0.06%
 37	   2261	  0.07%
 38	   2309	  0.07%
 39	   2555	  0.08%
 40	   2795	  0.09%
 41	   3253	  0.10%
 42	   4373	  0.14%
 43	   5843	  0.18%
 44	  10139	  0.31%
 45	  12669	  0.39%
 46	  11056	  0.34%
 47	  16700	  0.52%
 48	  35425	  1.10%
 49	  52380	  1.62%
 50	3055601	 94.51%
3233013 reads passed initial QC


criterion=sequence-density
sequence-density=80.96
sequence-density-rank=1
fanout-score=19.51
fanout-score-rank=1
prefix-density=82.84
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC


criterion=fanout-score
sequence-density=80.96
sequence-density-rank=1
fanout-score=19.51
fanout-score-rank=1
prefix-density=82.84
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC -o SRR5030915 -
Input file:	STDIN
trimmed:	SRR5030915-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:43:57 2020 >> started

Wed Mar  4 06:44:00 2020 >> done (3.418s)
3153186 reads processed; of these:
 151498 ( 4.80%) short reads filtered out after trimming by size control
   9500 ( 0.30%) empty reads filtered out after trimming by size control
2992188 (94.89%) reads available; of these:
2644358 (88.38%) trimmed reads available after processing
 347830 (11.62%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  18488	  0.62%
 19	  33454	  1.12%
 20	 137743	  4.60%
 21	 314391	 10.51%
 22	 940459	 31.43%
 23	 488770	 16.33%
 24	 168540	  5.63%
 25	  31100	  1.04%
 26	  16171	  0.54%
 27	  14964	  0.50%
 28	  17536	  0.59%
 29	  14048	  0.47%
 30	  23447	  0.78%
 31	  31252	  1.04%
 32	  32250	  1.08%
 33	  60560	  2.02%
 34	  32565	  1.09%
 35	  24977	  0.83%
 36	  28104	  0.94%
 37	  14343	  0.48%
 38	  15833	  0.53%
 39	  15366	  0.51%
 40	  22046	  0.74%
 41	  29641	  0.99%
 42	  32089	  1.07%
 43	  24456	  0.82%
 44	  19752	  0.66%
 45	  25258	  0.84%
 46	  20577	  0.69%
 47	  15724	  0.53%
 48	   3391	  0.11%
 49	  12446	  0.42%
 50	 312447	 10.44%


criterion=sequence-density
sequence-density=4.72
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=19
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCTTTAACCTGTGAA


criterion=fanout-score
sequence-density=0.41
sequence-density-rank=31
fanout-score=16.67
fanout-score-rank=1
prefix-density=6.77
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 06:44:21
                             Started mapping on |	Mar 04 06:44:21
                                    Finished on |	Mar 04 06:44:43
       Mapping speed, Million of reads per hour |	502.69

                          Number of input reads |	3072015
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1555220
                        Uniquely mapped reads % |	50.63%
                          Average mapped length |	27.46
                       Number of splices: Total |	1506
            Number of splices: Annotated (sjdb) |	212
                       Number of splices: GT/AG |	1384
                       Number of splices: GC/AG |	19
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	103
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.71
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.10
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1163620
             % of reads mapped to multiple loci |	37.88%
        Number of reads mapped to too many loci |	76365
             % of reads mapped to too many loci |	2.49%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.30%
                     % of reads unmapped: other |	0.71%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	353175	353175	353175
N_multimapping	1163620	1163620	1163620
N_noFeature	66563	83468	1198829
N_ambiguous	823150	523601	14319
UnstrandedReadsAssigned:665507 PositiveStrandReadsAssigned:948151 NegativeStrandReadsAssigned:342072
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030915 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030915-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,072,015 reads, 603,783 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,140 rounds

 109283 SRR5030915.ke.tsv
  52636 SRR5030915.se.tsv
 161919 total
==> SRR5030915.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030915.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030915 completed mapping pipeline successfully
