Starting /dee2/code/volunteer_pipeline.sh SRR5031011
    current disk space = 160535199744
    free memory = 73919924 
SRR5031011 SRAfilesize
a0238bbb817b6dbf0045e785854bc5e6  SRR5031011.sra
SRR5031011.sra file validated
SRR5031011 is single end
SRR5031011 is conventional basespace
SRR5031011 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38025	33.0	31.0	34.0	31.0	34.0
2	32.5935	34.0	31.0	34.0	31.0	34.0
3	32.5035	34.0	31.0	34.0	31.0	34.0
4	36.13625	37.0	35.0	37.0	35.0	37.0
5	36.15925	37.0	37.0	37.0	35.0	37.0
6	35.8585	37.0	35.0	37.0	33.0	37.0
7	35.9005	37.0	35.0	37.0	35.0	37.0
8	36.0445	37.0	35.0	37.0	35.0	37.0
9	37.66025	39.0	37.0	39.0	35.0	39.0
10	37.5195	39.0	37.0	39.0	35.0	39.0
11	37.2435	39.0	37.0	39.0	33.0	39.0
12	37.69725	39.0	37.0	39.0	35.0	39.0
13	37.56725	39.0	37.0	39.0	35.0	39.0
14	39.08425	40.0	38.0	41.0	36.0	41.0
15	38.9605	41.0	38.0	41.0	35.0	41.0
16	38.99025	40.0	38.0	41.0	35.0	41.0
17	39.06725	40.0	38.0	41.0	36.0	41.0
18	38.9575	41.0	38.0	41.0	35.0	41.0
19	38.713	40.0	38.0	41.0	34.0	41.0
20	38.8205	40.0	38.0	41.0	35.0	41.0
21	38.9235	40.0	38.0	41.0	35.0	41.0
22	38.8385	40.0	39.0	41.0	35.0	41.0
23	38.485	40.0	38.0	41.0	34.0	41.0
24	38.84175	40.0	38.0	41.0	35.0	41.0
25	38.9235	41.0	39.0	41.0	35.0	41.0
26	38.853	40.0	38.0	41.0	35.0	41.0
27	38.9035	41.0	39.0	41.0	35.0	41.0
28	38.76325	40.0	38.0	41.0	35.0	41.0
29	38.40725	40.0	38.0	41.0	34.0	41.0
30	38.439	40.0	38.0	41.0	34.0	41.0
31	38.686	40.0	38.0	41.0	34.0	41.0
32	38.724	40.0	38.0	41.0	35.0	41.0
33	38.42475	40.0	38.0	41.0	34.0	41.0
34	38.16825	40.0	38.0	41.0	33.0	41.0
35	38.047	40.0	38.0	41.0	33.0	41.0
36	37.8345	40.0	37.0	41.0	33.0	41.0
37	37.54125	40.0	37.0	41.0	31.0	41.0
38	37.5835	40.0	37.0	41.0	33.0	41.0
39	37.91175	40.0	38.0	41.0	33.0	41.0
40	37.9715	40.0	38.0	41.0	33.0	41.0
41	38.07675	40.0	38.0	41.0	33.0	41.0
42	38.00975	40.0	38.0	41.0	33.0	41.0
43	37.4435	40.0	37.0	41.0	32.0	41.0
44	37.713	40.0	38.0	41.0	33.0	41.0
45	37.6725	40.0	37.0	41.0	33.0	41.0
46	37.57725	40.0	37.0	41.0	32.0	41.0
47	37.23475	40.0	37.0	41.0	31.0	41.0
48	36.9625	40.0	37.0	41.0	31.0	41.0
49	36.88975	40.0	36.0	41.0	31.0	41.0
50	36.465	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	1.0
15	2.0
16	2.0
17	0.0
18	1.0
19	4.0
20	1.0
21	6.0
22	8.0
23	9.0
24	15.0
25	12.0
26	16.0
27	23.0
28	24.0
29	35.0
30	50.0
31	51.0
32	85.0
33	93.0
34	129.0
35	182.0
36	234.0
37	392.0
38	688.0
39	1934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	8.225	22.325	55.65	13.8
2	24.26599749058971	27.00125470514429	22.00752823086575	26.72521957340025
3	16.975	19.475	21.475	42.075
4	26.325	37.2	13.950000000000001	22.525000000000002
5	21.6	37.35	28.575	12.475
6	35.3	15.825	36.6	12.275
7	15.125	25.25	23.9	35.725
8	24.099999999999998	39.5	15.7	20.7
9	19.45	37.6	30.0	12.950000000000001
10	20.925	20.674999999999997	25.624999999999996	32.775
11	29.849999999999998	11.375	15.85	42.925000000000004
12	16.8	15.975	24.5	42.725
13	19.35	21.375	37.325	21.95
14	38.4	22.85	24.55	14.2
15	38.375	21.325	28.225	12.075
16	13.25	25.55	23.925	37.275000000000006
17	25.95	22.2	38.4	13.450000000000001
18	29.825000000000003	18.5	27.55	24.125
19	38.275	13.225000000000001	30.349999999999998	18.15
20	47.375	18.0	22.650000000000002	11.975
21	22.525000000000002	26.174999999999997	24.0	27.3
22	31.2	14.549999999999999	46.375	7.875
23	24.25	13.775	47.575	14.399999999999999
24	52.025	13.850000000000001	27.650000000000002	6.4750000000000005
25	54.825	21.975	15.25	7.95
26	28.7	49.225	12.2	9.875
27	13.975000000000001	52.47500000000001	23.275000000000002	10.274999999999999
28	9.025	25.575	51.800000000000004	13.600000000000001
29	10.375	11.3	59.9	18.425
30	9.425	5.35	38.3	46.925
31	13.925	4.5249999999999995	46.775	34.775
32	25.674999999999997	5.65	22.725	45.95
33	56.45	6.15	13.25	24.15
34	67.07499999999999	6.925000000000001	12.625	13.375
35	66.625	7.575	17.0	8.799999999999999
36	40.550000000000004	7.5249999999999995	39.225	12.7
37	46.575	8.825	21.925	22.675
38	23.1	14.224999999999998	12.4	50.275000000000006
39	14.549999999999999	21.6	8.325000000000001	55.525000000000006
40	14.05	49.825	8.975	27.150000000000002
41	23.549999999999997	53.474999999999994	8.075000000000001	14.899999999999999
42	51.525	30.875000000000004	8.674999999999999	8.924999999999999
43	56.725	27.375	7.35	8.55
44	31.55	50.125	6.8500000000000005	11.475
45	16.45	53.474999999999994	11.875	18.2
46	10.525	27.125	17.325	45.025
47	7.35	14.499999999999998	40.25	37.9
48	8.924999999999999	11.35	21.3	58.425000000000004
49	11.700000000000001	18.55	12.625	57.125
50	17.375	40.225	11.200000000000001	31.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	1.0
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	2.0
30	3.0
31	8.5
32	14.0
33	14.5
34	15.0
35	15.5
36	16.0
37	24.0
38	32.0
39	30.0
40	28.0
41	37.5
42	47.0
43	83.5
44	120.0
45	167.0
46	214.0
47	397.5
48	581.0
49	620.0
50	659.0
51	837.0
52	1015.0
53	692.0
54	369.0
55	358.5
56	348.0
57	305.5
58	263.0
59	166.0
60	69.0
61	51.0
62	33.0
63	45.0
64	57.0
65	43.0
66	29.0
67	20.0
68	11.0
69	11.0
70	11.0
71	30.0
72	49.0
73	26.0
74	3.0
75	2.5
76	2.0
77	3.0
78	4.0
79	4.0
80	4.0
81	2.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.375
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.1
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80314960629921	28.499999999999996
2	11.15485564304462	8.5
3	4.199475065616798	4.8
4	1.574803149606299	2.4
5	1.5091863517060367	2.875
6	1.1811023622047243	2.7
7	0.7217847769028871	1.925
8	0.39370078740157477	1.2
9	0.39370078740157477	1.35
>10	3.608923884514436	25.874999999999996
>50	0.19685039370078738	4.025
>100	0.26246719160104987	15.85
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	233	5.825	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	152	3.8	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	129	3.225	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	120	3.0	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	52	1.3	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	49	1.225	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	27	0.675	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	23	0.575	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	22	0.5499999999999999	No Hit
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	21	0.525	RNA PCR Primer, Index 7 (100% over 35bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	19	0.475	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC	19	0.475	RNA PCR Primer, Index 31 (100% over 50bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	17	0.42500000000000004	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC	14	0.35000000000000003	RNA PCR Primer, Index 31 (100% over 44bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	9	0.22499999999999998	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	6	0.15	No Hit
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	5	0.125	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	5	0.125	No Hit
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	5	0.125	No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
CAGCAGCACACTGTGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.5	0.0	0.0	0.0
2	0.0	0.5	0.0	0.0	0.0
3	0.0	0.525	0.0	0.0	0.0
4	0.0	0.55	0.0	0.0	0.0
5	0.0	0.625	0.0	0.0	0.0
6	0.0	0.725	0.0	0.0	0.0
7	0.0	1.15	0.0	0.0	0.0
8	0.0	1.35	0.0	0.0	0.0
9	0.0	1.4	0.0	0.0	0.0
10	0.0	1.475	0.0	0.0	0.0
11	0.0	1.525	0.0	0.0	0.0
12	0.0	1.775	0.0	0.0	0.0
13	0.0	2.0	0.0	0.0	0.0
14	0.0	2.3	0.0	0.0	0.0
15	0.0	2.6	0.0	0.0	0.0
16	0.0	3.525	0.0	0.0	0.0
17	0.0	4.175	0.0	0.0	0.0
18	0.0	5.05	0.0	0.0	0.0
19	0.0	5.55	0.0	0.0	0.0
20	0.0	6.925	0.0	0.0	0.0
21	0.0	11.7	0.0	0.0	0.0
22	0.0	22.975	0.0	0.0	0.0
23	0.0	55.625	0.0	0.0	0.0
24	0.0	70.475	0.0	0.0	0.0
25	0.0	76.325	0.0	0.0	0.0
26	0.0	77.5	0.0	0.0	0.0
27	0.0	78.075	0.0	0.0	0.0
28	0.0	78.625	0.0	0.0	0.0
29	0.0	79.175	0.0	0.0	0.0
30	0.0	79.75	0.0	0.0	0.0
31	0.0	80.6	0.0	0.0	0.0
32	0.0	82.0	0.0	0.0	0.0
33	0.0	83.175	0.0	0.0	0.0
34	0.0	85.025	0.0	0.0	0.0
35	0.0	85.875	0.0	0.0	0.0
36	0.0	86.325	0.0	0.0	0.0
37	0.0	87.075	0.0	0.0	0.0
38	0.0	87.45	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCAG	45	2.728484E-10	44.000004	8
ATCCAGG	45	2.728484E-10	44.000004	9
ATAGGCT	45	2.728484E-10	44.000004	16
AGTGGAA	20	6.952051E-4	44.000004	21
GGATCCG	20	6.952051E-4	44.000004	3
CCGTCTG	20	6.952051E-4	44.000004	7
TCGGATC	20	6.952051E-4	44.000004	1
CGGATCC	20	6.952051E-4	44.000004	2
TCCAGGA	45	2.728484E-10	44.000004	10
AGTAATC	45	2.728484E-10	44.000004	5
GATCCGT	20	6.952051E-4	44.000004	4
TAATCCA	45	2.728484E-10	44.000004	7
AGGTTGT	20	6.952051E-4	44.000004	10
TCCGTCT	20	6.952051E-4	44.000004	6
ATCCGTC	20	6.952051E-4	44.000004	5
GTAATCC	45	2.728484E-10	44.000004	6
TGAGCTT	20	6.952051E-4	44.000004	12
CGTCTGA	20	6.952051E-4	44.000004	8
GATAGGC	45	2.728484E-10	44.000004	15
CCAGGAT	45	2.728484E-10	44.000004	11
>>END_MODULE
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511676 spots for SRR5031011.sra
Written 511676 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
Read 511673 spots for SRR5031011.sra
Written 511673 spots for SRR5031011.sra
SRR ids: ['SRR5031011.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6mlm6jrv
SRR5031011.sra spots: 6140079
blocks: [[1, 511673], [511674, 1023346], [1023347, 1535019], [1535020, 2046692], [2046693, 2558365], [2558366, 3070038], [3070039, 3581711], [3581712, 4093384], [4093385, 4605057], [4605058, 5116730], [5116731, 5628403], [5628404, 6140079]]
SRR5031011 file size 1063162
SRR5031011 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031011 SRR5031011_1.fastq
Input file:	SRR5031011_1.fastq
trimmed:	SRR5031011-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:06:12 2020 >> started

Wed Mar  4 07:06:16 2020 >> done (4.682s)
6140079 reads processed; of these:
    355 ( 0.01%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
6139721 (99.99%) reads available; of these:
 351261 ( 5.72%) trimmed reads available after processing
5788460 (94.28%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    104	  0.00%
 19	    153	  0.00%
 20	    299	  0.00%
 21	    412	  0.01%
 22	    746	  0.01%
 23	    842	  0.01%
 24	    769	  0.01%
 25	   1136	  0.02%
 26	    921	  0.02%
 27	   1220	  0.02%
 28	   1435	  0.02%
 29	   2667	  0.04%
 30	   1456	  0.02%
 31	   1496	  0.02%
 32	   1773	  0.03%
 33	   2493	  0.04%
 34	   3193	  0.05%
 35	   6309	  0.10%
 36	   5237	  0.09%
 37	   6076	  0.10%
 38	   5384	  0.09%
 39	   5718	  0.09%
 40	   6497	  0.11%
 41	   7116	  0.12%
 42	   9393	  0.15%
 43	  13730	  0.22%
 44	  17960	  0.29%
 45	  22216	  0.36%
 46	  30858	  0.50%
 47	  39973	  0.65%
 48	  71151	  1.16%
 49	  82528	  1.34%
 50	5788460	 94.28%
6139721 reads passed initial QC


criterion=sequence-density
sequence-density=87.87
sequence-density-rank=1
fanout-score=19.68
fanout-score-rank=1
prefix-density=89.78
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC


criterion=fanout-score
sequence-density=87.87
sequence-density-rank=1
fanout-score=19.68
fanout-score-rank=1
prefix-density=89.78
prefix-fanout=19.3
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC -o SRR5031011 -
Input file:	STDIN
trimmed:	SRR5031011-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:06:51 2020 >> started

Wed Mar  4 07:07:06 2020 >> done (15.442s)
6000182 reads processed; of these:
 292846 ( 4.88%) short reads filtered out after trimming by size control
  20090 ( 0.33%) empty reads filtered out after trimming by size control
5687246 (94.78%) reads available; of these:
5460248 (96.01%) trimmed reads available after processing
 226998 ( 3.99%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  31134	  0.55%
 19	  71886	  1.26%
 20	 284753	  5.01%
 21	 661810	 11.64%
 22	1953121	 34.34%
 23	 982444	 17.27%
 24	 368360	  6.48%
 25	  64532	  1.13%
 26	  34632	  0.61%
 27	  33007	  0.58%
 28	  39127	  0.69%
 29	  30613	  0.54%
 30	  50032	  0.88%
 31	  74393	  1.31%
 32	  73477	  1.29%
 33	 117750	  2.07%
 34	  63765	  1.12%
 35	  46125	  0.81%
 36	  50326	  0.88%
 37	  29888	  0.53%
 38	  34933	  0.61%
 39	  30951	  0.54%
 40	  46035	  0.81%
 41	  60347	  1.06%
 42	  65176	  1.15%
 43	  49570	  0.87%
 44	  40027	  0.70%
 45	  45039	  0.79%
 46	  33979	  0.60%
 47	  22941	  0.40%
 48	   2590	  0.05%
 49	   5227	  0.09%
 50	 189256	  3.33%


criterion=sequence-density
sequence-density=5.73
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=23
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCCTGAGACCCTTTAACCTGTGAA


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=32
fanout-score=15.09
fanout-score-rank=1
prefix-density=6.35
prefix-fanout=1.0
sequence=CTGAGCTTGGCATT
                                 Started job on |	Mar 04 07:08:05
                             Started mapping on |	Mar 04 07:08:06
                                    Finished on |	Mar 04 07:08:51
       Mapping speed, Million of reads per hour |	466.14

                          Number of input reads |	5826785
                      Average input read length |	26
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2848734
                        Uniquely mapped reads % |	48.89%
                          Average mapped length |	24.61
                       Number of splices: Total |	2962
            Number of splices: Annotated (sjdb) |	336
                       Number of splices: GT/AG |	2674
                       Number of splices: GC/AG |	26
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	260
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.69
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2348472
             % of reads mapped to multiple loci |	40.30%
        Number of reads mapped to too many loci |	156071
             % of reads mapped to too many loci |	2.68%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.30%
                     % of reads unmapped: other |	0.82%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	629579	629579	629579
N_multimapping	2348472	2348472	2348472
N_noFeature	131528	162951	2191763
N_ambiguous	1502500	953298	23619
UnstrandedReadsAssigned:1214706 PositiveStrandReadsAssigned:1732485 NegativeStrandReadsAssigned:633352
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR5031011 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031011-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,826,785 reads, 1,134,053 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,205 rounds

 109283 SRR5031011.ke.tsv
  52636 SRR5031011.se.tsv
 161919 total
==> SRR5031011.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031011.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031011 completed mapping pipeline successfully
