Starting /dee2/code/volunteer_pipeline.sh SRR5030910
    current disk space = 160655278080
    free memory = 73948704 
SRR5030910 SRAfilesize
81de702476b6fb4926192dbf1523e6cf  SRR5030910.sra
SRR5030910.sra file validated
SRR5030910 is single end
SRR5030910 is conventional basespace
SRR5030910 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030910_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3595	33.0	31.0	34.0	31.0	34.0
2	32.64225	34.0	31.0	34.0	31.0	34.0
3	32.59275	34.0	31.0	34.0	31.0	34.0
4	36.1885	37.0	35.0	37.0	35.0	37.0
5	36.318	37.0	37.0	37.0	35.0	37.0
6	36.08475	37.0	35.0	37.0	35.0	37.0
7	36.08525	37.0	35.0	37.0	35.0	37.0
8	36.14825	37.0	35.0	37.0	35.0	37.0
9	37.903	39.0	38.0	39.0	35.0	39.0
10	37.90475	39.0	38.0	39.0	35.0	39.0
11	37.87525	39.0	38.0	39.0	35.0	39.0
12	37.758	39.0	38.0	39.0	35.0	39.0
13	37.7535	39.0	38.0	39.0	35.0	39.0
14	39.27925	41.0	39.0	41.0	36.0	41.0
15	39.22975	41.0	39.0	41.0	36.0	41.0
16	39.1805	40.0	38.0	41.0	36.0	41.0
17	39.099	40.0	38.0	41.0	36.0	41.0
18	39.308	41.0	39.0	41.0	36.0	41.0
19	39.09375	40.0	39.0	41.0	35.0	41.0
20	39.0415	40.0	39.0	41.0	36.0	41.0
21	39.07925	40.0	39.0	41.0	36.0	41.0
22	39.349	41.0	39.0	41.0	36.0	41.0
23	39.256	40.0	39.0	41.0	36.0	41.0
24	38.5735	40.0	38.0	41.0	34.0	41.0
25	38.7775	41.0	39.0	41.0	34.0	41.0
26	38.9315	41.0	38.0	41.0	35.0	41.0
27	39.046	41.0	39.0	41.0	35.0	41.0
28	38.801	40.0	38.0	41.0	35.0	41.0
29	38.38	40.0	38.0	41.0	33.0	41.0
30	38.652	40.0	38.0	41.0	34.0	41.0
31	39.03075	41.0	39.0	41.0	35.0	41.0
32	39.0045	41.0	39.0	41.0	35.0	41.0
33	38.0865	40.0	38.0	41.0	33.0	41.0
34	38.141	40.0	38.0	41.0	33.0	41.0
35	37.80125	40.0	37.0	41.0	33.0	41.0
36	32.3505	37.0	26.0	40.0	15.0	41.0
37	35.05625	37.0	33.0	39.0	28.0	41.0
38	36.85325	38.0	36.0	40.0	31.0	41.0
39	37.1095	39.0	37.0	40.0	31.0	41.0
40	37.26625	39.0	37.0	40.0	32.0	41.0
41	37.0045	39.0	36.0	40.0	31.0	41.0
42	37.114	39.0	36.0	40.0	31.0	41.0
43	37.08025	39.0	37.0	40.0	32.0	41.0
44	37.1575	40.0	37.0	41.0	32.0	41.0
45	36.6415	39.0	36.0	40.0	31.0	41.0
46	34.1635	38.0	33.0	40.0	23.0	41.0
47	35.54825	38.0	35.0	40.0	28.0	41.0
48	35.9775	39.0	35.0	40.0	30.0	41.0
49	34.75975	38.0	34.0	40.0	27.0	41.0
50	30.47175	34.0	27.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.0
20	3.0
21	6.0
22	5.0
23	10.0
24	10.0
25	20.0
26	22.0
27	27.0
28	25.0
29	40.0
30	58.0
31	65.0
32	79.0
33	118.0
34	163.0
35	209.0
36	324.0
37	568.0
38	1056.0
39	1186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	9.530975670930525	22.121896162528216	54.30147980938048	14.045648357160772
2	23.225	26.0	24.3	26.474999999999998
3	16.737553164873656	18.689016762571928	22.266700025018764	42.30673004753565
4	24.975	37.475	14.374999999999998	23.175
5	20.025000000000002	39.825	28.125	12.025
6	36.075	17.0	35.475	11.450000000000001
7	17.974999999999998	22.2	23.799999999999997	36.025
8	23.325000000000003	42.05	14.899999999999999	19.725
9	17.925	37.95	31.025000000000002	13.100000000000001
10	21.85	18.45	26.950000000000003	32.75
11	29.925	12.950000000000001	15.174999999999999	41.949999999999996
12	15.625	18.675	24.175	41.525
13	20.275000000000002	23.525	35.949999999999996	20.25
14	37.875	24.3	24.125	13.700000000000001
15	36.825	22.975	28.499999999999996	11.700000000000001
16	14.224999999999998	26.625	21.675	37.475
17	24.925	22.775000000000002	39.025	13.275
18	29.15	18.9	27.575	24.375
19	38.224999999999994	14.249999999999998	30.675	16.85
20	45.95	19.45	22.85	11.75
21	21.25	25.775	23.200000000000003	29.775000000000002
22	32.324999999999996	12.55	45.75	9.375
23	23.200000000000003	13.8	47.775	15.225
24	51.425	12.525	28.4	7.6499999999999995
25	54.175	20.125	14.6	11.1
26	30.7	45.725	13.075000000000001	10.5
27	15.475	51.949999999999996	20.674999999999997	11.899999999999999
28	10.45	26.625	49.9	13.025
29	10.274999999999999	13.15	59.550000000000004	17.025000000000002
30	10.225	6.25	36.449999999999996	47.075
31	12.925	4.8	48.35	33.925
32	25.124999999999996	5.75	23.325000000000003	45.800000000000004
33	53.37499999999999	7.825	13.325000000000001	25.474999999999998
34	63.6	7.6	13.700000000000001	15.1
35	66.0	8.05	15.7	10.25
36	45.6	8.450000000000001	34.599999999999994	11.35
37	46.550000000000004	9.725	22.6	21.125
38	25.15	13.175	13.4	48.275
39	16.25	20.549999999999997	9.225	53.974999999999994
40	13.350000000000001	48.65	11.15	26.85
41	21.05	54.05	8.7	16.2
42	51.9	30.575000000000003	10.05	7.475
43	56.10000000000001	27.224999999999998	7.3	9.375
44	32.375	48.575	8.025	11.025
45	16.125	55.675	11.25	16.950000000000003
46	11.05	28.9	17.65	42.4
47	8.275	15.075	41.6	35.05
48	10.75	11.075	21.825	56.35
49	11.899999999999999	18.775	14.549999999999999	54.775
50	18.125	38.224999999999994	11.55	32.1
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	2.0
28	3.0
29	2.5
30	2.0
31	9.0
32	16.0
33	18.5
34	21.0
35	27.5
36	34.0
37	51.5
38	69.0
39	58.5
40	48.0
41	50.5
42	53.0
43	82.5
44	112.0
45	173.5
46	235.0
47	378.5
48	522.0
49	597.0
50	672.0
51	818.5
52	965.0
53	656.0
54	347.0
55	345.5
56	344.0
57	302.0
58	260.0
59	155.0
60	50.0
61	46.5
62	43.0
63	58.5
64	74.0
65	54.0
66	34.0
67	21.0
68	8.0
69	18.0
70	28.0
71	34.5
72	41.0
73	24.0
74	7.0
75	4.5
76	2.0
77	2.5
78	3.0
79	2.0
80	1.0
81	2.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.325
2	0.0
3	0.075
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.925000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93364928909952	27.3
2	9.27555856465809	6.8500000000000005
3	5.077860528097495	5.625
4	2.640487474610697	3.9
5	1.5572105619498984	2.875
6	1.2186865267433988	2.7
7	0.9478672985781991	2.45
8	0.5416384563303994	1.6
9	0.3385240352064997	1.125
>10	4.129993229519296	30.625000000000004
>50	0.13540961408259986	3.95
>100	0.2031144211238998	11.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	170	4.25	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	166	4.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	104	2.6	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	99	2.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	59	1.4749999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	50	1.25	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	47	1.175	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	46	1.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	46	1.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	33	0.8250000000000001	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	27	0.675	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	26	0.65	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	26	0.65	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	22	0.5499999999999999	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	21	0.525	No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC	21	0.525	RNA PCR Primer, Index 5 (97% over 35bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC	14	0.35000000000000003	RNA PCR Primer, Index 47 (100% over 44bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	13	0.325	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	12	0.3	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (96% over 28bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	11	0.27499999999999997	No Hit
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	11	0.27499999999999997	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 33bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	11	0.27499999999999997	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	10	0.25	No Hit
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	10	0.25	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	8	0.2	No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	7	0.17500000000000002	No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGC	6	0.15	RNA PCR Primer, Index 47 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	6	0.15	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAG	6	0.15	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	6	0.15	No Hit
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	5	0.125	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	5	0.125	No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	5	0.125	RNA PCR Primer, Index 3 (100% over 34bp)
TCCCTGAGACCCTTTAACCTGTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	5	0.125	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.175	0.0	0.0	0.0
2	0.0	0.2	0.0	0.0	0.0
3	0.0	0.225	0.0	0.0	0.0
4	0.0	0.25	0.0	0.0	0.0
5	0.0	0.275	0.0	0.0	0.0
6	0.0	0.3	0.0	0.0	0.0
7	0.0	0.725	0.0	0.0	0.0
8	0.0	0.8	0.0	0.0	0.0
9	0.0	0.85	0.0	0.0	0.0
10	0.0	0.95	0.0	0.0	0.0
11	0.0	1.2	0.0	0.0	0.0
12	0.0	1.45	0.0	0.0	0.0
13	0.0	1.65	0.0	0.0	0.0
14	0.0	1.875	0.0	0.0	0.0
15	0.0	2.175	0.0	0.0	0.0
16	0.0	3.075	0.0	0.0	0.0
17	0.0	3.45	0.0	0.0	0.0
18	0.0	4.775	0.0	0.0	0.0
19	0.0	5.175	0.0	0.0	0.0
20	0.0	6.25	0.0	0.0	0.0
21	0.0	10.2	0.0	0.0	0.0
22	0.0	19.75	0.0	0.0	0.0
23	0.0	51.575	0.0	0.0	0.0
24	0.0	66.525	0.0	0.0	0.0
25	0.0	72.55	0.0	0.0	0.0
26	0.0	73.525	0.0	0.0	0.0
27	0.0	73.825	0.0	0.0	0.0
28	0.0	74.325	0.0	0.0	0.0
29	0.0	74.55	0.0	0.0	0.0
30	0.0	74.9	0.0	0.0	0.0
31	0.0	75.775	0.0	0.0	0.0
32	0.0	76.7	0.0	0.0	0.0
33	0.0	77.5	0.0	0.0	0.0
34	0.0	79.5	0.0	0.0	0.0
35	0.0	80.0	0.0	0.0	0.0
36	0.0	80.625	0.0	0.0	0.0
37	0.0	81.375	0.0	0.0	0.0
38	0.0	81.95	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACC	20	6.952051E-4	44.000004	5
GAGGTAG	20	6.952051E-4	44.000004	2
AATCCAG	45	2.728484E-10	44.000004	8
CTGAGCT	20	6.952051E-4	44.000004	11
ATCCAGG	45	2.728484E-10	44.000004	9
CTTTAAC	20	6.952051E-4	44.000004	12
CGGGGGC	20	6.952051E-4	44.000004	32
ATAGGCT	40	5.315087E-9	44.000004	16
GAGCTTG	20	6.952051E-4	44.000004	13
GGATCCG	20	6.952051E-4	44.000004	3
CCGTCTG	20	6.952051E-4	44.000004	7
TCGGATC	20	6.952051E-4	44.000004	1
CATTCAA	20	6.952051E-4	44.000004	3
CGGATCC	20	6.952051E-4	44.000004	2
TCCAGGA	45	2.728484E-10	44.000004	10
GGATAGG	45	2.728484E-10	44.000004	14
TAACCTG	20	6.952051E-4	44.000004	15
GGGGGCC	20	6.952051E-4	44.000004	33
GATCCGT	20	6.952051E-4	44.000004	4
AGGCTTG	40	5.315087E-9	44.000004	18
>>END_MODULE
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
Read 284174 spots for SRR5030910.sra
Written 284174 spots for SRR5030910.sra
Read 284163 spots for SRR5030910.sra
Written 284163 spots for SRR5030910.sra
SRR ids: ['SRR5030910.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_st2wdf93
SRR5030910.sra spots: 3409967
blocks: [[1, 284163], [284164, 568326], [568327, 852489], [852490, 1136652], [1136653, 1420815], [1420816, 1704978], [1704979, 1989141], [1989142, 2273304], [2273305, 2557467], [2557468, 2841630], [2841631, 3125793], [3125794, 3409967]]
SRR5030910 file size 589945
SRR5030910 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030910 SRR5030910_1.fastq
Input file:	SRR5030910_1.fastq
trimmed:	SRR5030910-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:42:24 2020 >> started

Wed Mar  4 06:42:26 2020 >> done (2.318s)
3409967 reads processed; of these:
    142 ( 0.00%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
3409820 (100.00%) reads available; of these:
 254453 ( 7.46%) trimmed reads available after processing
3155367 (92.54%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     46	  0.00%
 19	     47	  0.00%
 20	    126	  0.00%
 21	    150	  0.00%
 22	    287	  0.01%
 23	    352	  0.01%
 24	    360	  0.01%
 25	    439	  0.01%
 26	    378	  0.01%
 27	    534	  0.02%
 28	    654	  0.02%
 29	   1072	  0.03%
 30	    740	  0.02%
 31	    988	  0.03%
 32	   1354	  0.04%
 33	   2535	  0.07%
 34	   9696	  0.28%
 35	   5049	  0.15%
 36	   3628	  0.11%
 37	   3097	  0.09%
 38	   4790	  0.14%
 39	   4043	  0.12%
 40	   6889	  0.20%
 41	   5330	  0.16%
 42	   8960	  0.26%
 43	   6799	  0.20%
 44	  20024	  0.59%
 45	  16667	  0.49%
 46	  14591	  0.43%
 47	  18401	  0.54%
 48	  51855	  1.52%
 49	  64572	  1.89%
 50	3155367	 92.54%
3409820 reads passed initial QC


criterion=sequence-density
sequence-density=81.10
sequence-density-rank=1
fanout-score=19.04
fanout-score-rank=2
prefix-density=83.06
prefix-fanout=18.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC


criterion=fanout-score
sequence-density=2.03
sequence-density-rank=5
fanout-score=123.70
fanout-score-rank=1
prefix-density=83.29
prefix-fanout=3.0
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC -o SRR5030910 -
Input file:	STDIN
trimmed:	SRR5030910-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:42:47 2020 >> started

Wed Mar  4 06:42:55 2020 >> done (8.117s)
3326654 reads processed; of these:
 142982 ( 4.30%) short reads filtered out after trimming by size control
   8867 ( 0.27%) empty reads filtered out after trimming by size control
3174805 (95.44%) reads available; of these:
2815263 (88.68%) trimmed reads available after processing
 359542 (11.32%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  15734	  0.50%
 19	  35047	  1.10%
 20	 150389	  4.74%
 21	 355576	 11.20%
 22	1027259	 32.36%
 23	 503581	 15.86%
 24	 175356	  5.52%
 25	  28597	  0.90%
 26	  14689	  0.46%
 27	  14170	  0.45%
 28	  16176	  0.51%
 29	  13016	  0.41%
 30	  24564	  0.77%
 31	  32720	  1.03%
 32	  35062	  1.10%
 33	  58179	  1.83%
 34	  34165	  1.08%
 35	  24530	  0.77%
 36	  29508	  0.93%
 37	  15192	  0.48%
 38	  16402	  0.52%
 39	  16031	  0.50%
 40	  21720	  0.68%
 41	  30577	  0.96%
 42	  33717	  1.06%
 43	  25536	  0.80%
 44	  22774	  0.72%
 45	  28553	  0.90%
 46	  23141	  0.73%
 47	  18972	  0.60%
 48	   3798	  0.12%
 49	  11789	  0.37%
 50	 318285	 10.03%


criterion=sequence-density
sequence-density=9.10
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=1.43
sequence-density-rank=12
fanout-score=3.42
fanout-score-rank=1
prefix-density=0.04
prefix-fanout=3.4
sequence=TGAGGTAGTAGTTTGT
                                 Started job on |	Mar 04 06:43:26
                             Started mapping on |	Mar 04 06:43:27
                                    Finished on |	Mar 04 06:43:51
       Mapping speed, Million of reads per hour |	488.70

                          Number of input reads |	3257971
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1640547
                        Uniquely mapped reads % |	50.35%
                          Average mapped length |	27.36
                       Number of splices: Total |	1498
            Number of splices: Annotated (sjdb) |	221
                       Number of splices: GT/AG |	1339
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	143
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.55
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1257445
             % of reads mapped to multiple loci |	38.60%
        Number of reads mapped to too many loci |	78274
             % of reads mapped to too many loci |	2.40%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.02%
                     % of reads unmapped: other |	0.62%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	359979	359979	359979
N_multimapping	1257445	1257445	1257445
N_noFeature	70495	87935	1278754
N_ambiguous	861428	557555	16714
UnstrandedReadsAssigned:708624 PositiveStrandReadsAssigned:995057 NegativeStrandReadsAssigned:345079
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030910 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030910-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,257,971 reads, 651,561 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,143 rounds

 109283 SRR5030910.ke.tsv
  52636 SRR5030910.se.tsv
 161919 total
==> SRR5030910.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030910.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030910 completed mapping pipeline successfully
