Starting /dee2/code/volunteer_pipeline.sh SRR5031105
    current disk space = 160472416256
    free memory = 56733132 
SRR5031105 SRAfilesize
36d6d9682aa4b967af5dc123c9b76968  SRR5031105.sra
SRR5031105.sra file validated
SRR5031105 is single end
SRR5031105 is conventional basespace
SRR5031105 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98975	31.0	31.0	34.0	30.0	34.0
2	32.449	34.0	31.0	34.0	31.0	34.0
3	32.4655	34.0	31.0	34.0	31.0	34.0
4	36.18725	37.0	35.0	37.0	35.0	37.0
5	36.15	37.0	35.0	37.0	35.0	37.0
6	36.052	37.0	35.0	37.0	35.0	37.0
7	35.831	37.0	35.0	37.0	35.0	37.0
8	35.68225	37.0	35.0	37.0	33.0	37.0
9	36.89825	39.0	37.0	39.0	33.0	39.0
10	36.43575	39.0	35.0	39.0	32.0	39.0
11	37.36275	39.0	37.0	39.0	34.0	39.0
12	37.6735	39.0	37.0	39.0	35.0	39.0
13	37.4895	39.0	37.0	39.0	35.0	39.0
14	38.485	40.0	38.0	41.0	34.0	41.0
15	36.4985	39.0	36.0	41.0	27.0	41.0
16	36.32325	38.0	35.0	40.0	30.0	41.0
17	36.5925	39.0	36.0	40.0	30.0	41.0
18	38.08625	40.0	37.0	41.0	34.0	41.0
19	38.7765	40.0	38.0	41.0	35.0	41.0
20	38.48025	40.0	38.0	41.0	34.0	41.0
21	38.62175	40.0	38.0	41.0	35.0	41.0
22	37.41825	39.0	37.0	40.0	32.0	41.0
23	37.927	39.0	37.0	40.0	34.0	41.0
24	37.3885	39.0	37.0	40.0	31.0	41.0
25	38.169	40.0	38.0	41.0	34.0	41.0
26	38.1815	40.0	38.0	41.0	34.0	41.0
27	38.3765	40.0	38.0	41.0	34.0	41.0
28	38.54725	40.0	38.0	41.0	34.0	41.0
29	38.493	40.0	38.0	41.0	34.0	41.0
30	38.8475	40.0	38.0	41.0	35.0	41.0
31	38.827	40.0	38.0	41.0	35.0	41.0
32	38.694	40.0	38.0	41.0	35.0	41.0
33	38.9045	40.0	39.0	41.0	35.0	41.0
34	38.40725	40.0	38.0	41.0	33.0	41.0
35	37.35175	40.0	35.0	41.0	30.0	41.0
36	33.06125	35.0	30.0	40.0	23.0	41.0
37	35.01675	37.0	33.0	39.0	30.0	40.0
38	36.412	38.0	35.0	39.0	31.0	40.0
39	36.748	38.0	36.0	40.0	32.0	40.0
40	37.3145	39.0	37.0	40.0	33.0	41.0
41	37.66375	40.0	37.0	40.0	33.0	41.0
42	37.644	40.0	37.0	41.0	33.0	41.0
43	37.4965	40.0	37.0	41.0	33.0	41.0
44	36.56575	38.0	35.0	40.0	31.0	41.0
45	36.70875	39.0	36.0	40.0	31.0	41.0
46	36.97025	39.0	37.0	40.0	31.0	41.0
47	35.94225	38.0	35.0	40.0	28.0	41.0
48	37.071	39.0	37.0	40.0	31.0	41.0
49	37.12325	40.0	37.0	41.0	32.0	41.0
50	35.28725	38.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	3.0
18	2.0
19	1.0
20	4.0
21	5.0
22	6.0
23	5.0
24	12.0
25	13.0
26	14.0
27	13.0
28	26.0
29	36.0
30	45.0
31	60.0
32	80.0
33	134.0
34	190.0
35	262.0
36	445.0
37	781.0
38	1129.0
39	731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.906906906906906	24.6996996996997	56.18118118118118	12.212212212212211
2	22.372372372372375	28.053053053053052	17.39239239239239	32.18218218218218
3	23.799999999999997	22.475	19.325	34.4
4	32.0	29.225	11.625	27.150000000000002
5	21.925	41.949999999999996	26.724999999999998	9.4
6	36.925000000000004	13.575000000000001	35.375	14.124999999999998
7	11.525	22.95	25.775	39.75
8	27.3	30.9	12.675	29.125
9	20.5	37.525	28.299999999999997	13.675
10	18.475	23.425	27.250000000000004	30.85
11	32.6	9.0	15.35	43.05
12	14.000000000000002	15.9	30.25	39.85
13	31.65	16.950000000000003	33.775	17.625
14	35.675000000000004	28.4	21.0	14.924999999999999
15	34.625	18.575	28.925	17.875
16	16.925	19.125	20.549999999999997	43.4
17	24.15	19.55	40.675	15.625
18	25.525	16.7	30.049999999999997	27.725
19	36.9	12.875	31.95	18.275
20	47.3	11.899999999999999	29.75	11.05
21	21.7	19.650000000000002	30.65	28.000000000000004
22	28.325	10.25	51.925	9.5
23	28.050000000000004	10.525	49.075	12.35
24	56.3	10.725	25.424999999999997	7.55
25	54.25	26.224999999999998	13.375	6.15
26	26.625	53.225	12.65	7.5
27	14.35	51.800000000000004	25.95	7.9
28	8.799999999999999	24.45	54.525	12.225
29	8.774999999999999	10.025	59.975	21.224999999999998
30	7.55	4.625	39.875	47.949999999999996
31	11.5	3.2	49.4	35.9
32	28.825	3.95	20.95	46.275
33	61.175000000000004	5.7	11.450000000000001	21.675
34	70.125	4.7	12.5	12.675
35	68.875	5.775	18.525	6.825
36	46.550000000000004	5.7	36.925000000000004	10.825
37	48.0	7.6499999999999995	19.075	25.275
38	21.95	13.100000000000001	10.625	54.325
39	12.675	26.0	7.3	54.025
40	12.525	54.6	7.025	25.85
41	25.424999999999997	54.025	4.875	15.675
42	54.65	31.1	6.950000000000001	7.3
43	54.7	32.5	3.9	8.9
44	28.499999999999996	54.900000000000006	4.65	11.95
45	15.125	54.65	10.325	19.900000000000002
46	8.975	25.825	18.875	46.325
47	5.3	13.725000000000001	40.525	40.45
48	6.5	11.450000000000001	18.45	63.6
49	9.725	21.3	10.725	58.25
50	18.425	40.825	10.549999999999999	30.2
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	1.0
30	0.0
31	0.0
32	0.0
33	2.0
34	4.0
35	6.5
36	9.0
37	7.5
38	6.0
39	8.5
40	11.0
41	14.0
42	17.0
43	53.0
44	89.0
45	135.0
46	181.0
47	310.0
48	439.0
49	562.5
50	686.0
51	836.0
52	986.0
53	732.0
54	478.0
55	489.5
56	501.0
57	392.5
58	284.0
59	185.0
60	86.0
61	64.0
62	42.0
63	67.0
64	92.0
65	68.5
66	45.0
67	28.5
68	12.0
69	12.5
70	13.0
71	12.5
72	12.0
73	7.0
74	2.0
75	1.0
76	0.0
77	0.0
78	0.0
79	1.0
80	2.0
81	1.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.1
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3671875	29.325000000000003
2	9.635416666666668	7.3999999999999995
3	3.3854166666666665	3.9
4	2.604166666666667	4.0
5	1.2369791666666665	2.375
6	1.6276041666666667	3.75
7	0.5859375	1.575
8	0.6510416666666667	2.0
9	0.4557291666666667	1.575
>10	2.799479166666667	20.25
>50	0.32552083333333337	8.55
>100	0.32552083333333337	15.299999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	153	3.8249999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	105	2.625	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	88	2.1999999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	86	2.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	65	1.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	52	1.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	50	1.25	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	43	1.075	No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	32	0.8	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA	20	0.5	RNA PCR Primer, Index 7 (100% over 35bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	19	0.475	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	19	0.475	No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	16	0.4	RNA PCR Primer, Index 1 (100% over 26bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC	15	0.375	RNA PCR Primer, Index 29 (100% over 50bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGACGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (96% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC	11	0.27499999999999997	RNA PCR Primer, Index 29 (100% over 44bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AGCTGGTGTTGTGAATCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCCGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	8	0.2	No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGGCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 30bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	7	0.17500000000000002	No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
AAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TCGGATCCGTCTGAGCTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGACGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACCCTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	6	0.15	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTCGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGACCCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTTTTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGCCGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	6	0.15	No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA	6	0.15	RNA PCR Primer, Index 2 (97% over 36bp)
CAACGGAATCCCAACAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTTTACCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCACTGGAATTCTCGGGT	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TTCAAGTAATCCAGGATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGACTTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.5	0.0	0.0	0.0
2	0.0	0.55	0.0	0.0	0.0
3	0.0	0.65	0.0	0.0	0.0
4	0.0	0.65	0.0	0.0	0.0
5	0.0	0.75	0.0	0.0	0.0
6	0.0	0.775	0.0	0.0	0.0
7	0.0	1.25	0.0	0.0	0.0
8	0.0	1.45	0.0	0.0	0.0
9	0.0	1.475	0.0	0.0	0.0
10	0.0	1.575	0.0	0.0	0.0
11	0.0	1.65	0.0	0.0	0.0
12	0.0	1.775	0.0	0.0	0.0
13	0.0	1.975	0.0	0.0	0.0
14	0.0	2.15	0.0	0.0	0.0
15	0.0	2.475	0.0	0.0	0.0
16	0.0	3.425	0.0	0.0	0.0
17	0.0	4.0	0.0	0.0	0.0
18	0.0	5.15	0.0	0.0	0.0
19	0.0	5.725	0.0	0.0	0.0
20	0.0	6.8	0.0	0.0	0.0
21	0.0	11.825	0.0	0.0	0.0
22	0.0	26.9	0.0	0.0	0.0
23	0.0	61.45	0.0	0.0	0.0
24	0.0	76.1	0.0	0.0	0.0
25	0.0	82.1	0.0	0.0	0.0
26	0.0	83.75	0.0	0.0	0.0
27	0.0	84.375	0.0	0.0	0.0
28	0.0	85.025	0.0	0.0	0.0
29	0.0	85.625	0.0	0.0	0.0
30	0.0	85.825	0.0	0.0	0.0
31	0.0	86.575	0.0	0.0	0.0
32	0.0	87.5	0.0	0.0	0.0
33	0.0	88.275	0.0	0.0	0.0
34	0.0	90.6	0.0	0.0	0.0
35	0.0	91.1	0.0	0.0	0.0
36	0.0	91.5	0.0	0.0	0.0
37	0.0	92.025	0.0	0.0	0.0
38	0.0	92.225	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTTG	20	6.952051E-4	44.000004	18
GTTGAAG	20	6.952051E-4	44.000004	10
CAGTTGA	20	6.952051E-4	44.000004	8
CTGAGCT	45	2.728484E-10	44.000004	11
CCAGTTG	20	6.952051E-4	44.000004	7
CTCTTTT	20	6.952051E-4	44.000004	16
GAGCTTG	45	2.728484E-10	44.000004	13
GGATCCG	45	2.728484E-10	44.000004	3
CCGTCTG	45	2.728484E-10	44.000004	7
TCGGATC	45	2.728484E-10	44.000004	1
CGGATCC	45	2.728484E-10	44.000004	2
GCCAGTT	20	6.952051E-4	44.000004	6
CTTGGTG	20	6.952051E-4	44.000004	16
ACATTCA	45	2.728484E-10	44.000004	2
CTTGGCT	20	6.952051E-4	44.000004	16
GCTGCCA	20	6.952051E-4	44.000004	3
GCTGTCG	40	5.315087E-9	44.000004	11
GATCCGT	45	2.728484E-10	44.000004	4
ACCCTTT	20	6.952051E-4	44.000004	9
TGCCAGT	20	6.952051E-4	44.000004	5
>>END_MODULE
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121046 spots for SRR5031105.sra
Written 121046 spots for SRR5031105.sra
Read 121050 spots for SRR5031105.sra
Written 121050 spots for SRR5031105.sra
SRR ids: ['SRR5031105.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_o9132io2
SRR5031105.sra spots: 1452556
blocks: [[1, 121046], [121047, 242092], [242093, 363138], [363139, 484184], [484185, 605230], [605231, 726276], [726277, 847322], [847323, 968368], [968369, 1089414], [1089415, 1210460], [1210461, 1331506], [1331507, 1452556]]
SRR5031105 file size 250687
SRR5031105 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031105 SRR5031105_1.fastq
Input file:	SRR5031105_1.fastq
trimmed:	SRR5031105-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:24:48 2020 >> started

Wed Mar  4 07:24:49 2020 >> done (1.246s)
1452556 reads processed; of these:
    118 ( 0.01%) short reads filtered out after trimming by size control
      1 ( 0.00%) empty reads filtered out after trimming by size control
1452437 (99.99%) reads available; of these:
 112646 ( 7.76%) trimmed reads available after processing
1339791 (92.24%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     32	  0.00%
 19	     65	  0.00%
 20	    105	  0.01%
 21	    121	  0.01%
 22	    206	  0.01%
 23	    271	  0.02%
 24	    244	  0.02%
 25	    345	  0.02%
 26	    237	  0.02%
 27	    321	  0.02%
 28	    399	  0.03%
 29	    787	  0.05%
 30	    363	  0.02%
 31	    397	  0.03%
 32	    512	  0.04%
 33	   1057	  0.07%
 34	   1569	  0.11%
 35	   3990	  0.27%
 36	   2230	  0.15%
 37	   1966	  0.14%
 38	   1800	  0.12%
 39	   2080	  0.14%
 40	   2419	  0.17%
 41	   2706	  0.19%
 42	   3400	  0.23%
 43	   5589	  0.38%
 44	   5983	  0.41%
 45	   7045	  0.49%
 46	   9171	  0.63%
 47	   8359	  0.58%
 48	  26324	  1.81%
 49	  22553	  1.55%
 50	1339791	 92.24%
1452437 reads passed initial QC


criterion=sequence-density
sequence-density=92.18
sequence-density-rank=1
fanout-score=19.60
fanout-score-rank=2
prefix-density=93.02
prefix-fanout=19.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCC


criterion=fanout-score
sequence-density=3.85
sequence-density-rank=4
fanout-score=67.16
fanout-score-rank=1
prefix-density=92.92
prefix-fanout=2.8
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCC -o SRR5031105 -
Input file:	STDIN
trimmed:	SRR5031105-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:25:01 2020 >> started

Wed Mar  4 07:25:03 2020 >> done (2.310s)
1421202 reads processed; of these:
  70249 ( 4.94%) short reads filtered out after trimming by size control
   8366 ( 0.59%) empty reads filtered out after trimming by size control
1342587 (94.47%) reads available; of these:
1314586 (97.91%) trimmed reads available after processing
  28001 ( 2.09%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   7526	  0.56%
 19	  14684	  1.09%
 20	  83720	  6.24%
 21	 200613	 14.94%
 22	 509092	 37.92%
 23	 223077	 16.62%
 24	  95319	  7.10%
 25	  24452	  1.82%
 26	  10942	  0.81%
 27	   7864	  0.59%
 28	   7682	  0.57%
 29	   5905	  0.44%
 30	   9448	  0.70%
 31	  16451	  1.23%
 32	  10680	  0.80%
 33	  30654	  2.28%
 34	   8089	  0.60%
 35	   5387	  0.40%
 36	   6573	  0.49%
 37	   3404	  0.25%
 38	   3438	  0.26%
 39	   3125	  0.23%
 40	   4799	  0.36%
 41	   5858	  0.44%
 42	   6503	  0.48%
 43	   4042	  0.30%
 44	   2857	  0.21%
 45	   3668	  0.27%
 46	   2515	  0.19%
 47	   1876	  0.14%
 48	    330	  0.02%
 49	    534	  0.04%
 50	  21480	  1.60%


criterion=sequence-density
sequence-density=9.02
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=0.49
sequence-density-rank=32
fanout-score=22.07
fanout-score-rank=1
prefix-density=10.71
prefix-fanout=1.0
sequence=CTGAGCTTGGCA
                                 Started job on |	Mar 04 07:25:24
                             Started mapping on |	Mar 04 07:25:24
                                    Finished on |	Mar 04 07:25:50
       Mapping speed, Million of reads per hour |	190.22

                          Number of input reads |	1373822
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	736603
                        Uniquely mapped reads % |	53.62%
                          Average mapped length |	23.06
                       Number of splices: Total |	2975
            Number of splices: Annotated (sjdb) |	783
                       Number of splices: GT/AG |	2940
                       Number of splices: GC/AG |	20
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	14
                      Mismatch rate per base, % |	0.37%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.42
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	525025
             % of reads mapped to multiple loci |	38.22%
        Number of reads mapped to too many loci |	15824
             % of reads mapped to too many loci |	1.15%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.63%
                     % of reads unmapped: other |	0.39%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	112194	112194	112194
N_multimapping	525025	525025	525025
N_noFeature	47123	52240	508702
N_ambiguous	408765	206962	3997
UnstrandedReadsAssigned:280715 PositiveStrandReadsAssigned:477401 NegativeStrandReadsAssigned:223904
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031105 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031105-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,373,822 reads, 350,711 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 646 rounds

 109283 SRR5031105.ke.tsv
  52636 SRR5031105.se.tsv
 161919 total
==> SRR5031105.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031105.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031105 completed mapping pipeline successfully
