Starting /dee2/code/volunteer_pipeline.sh SRR5030949
    current disk space = 160611483648
    free memory = 73955504 
SRR5030949 SRAfilesize
c1f90725635b6f621292e1b8b1989ace  SRR5030949.sra
SRR5030949.sra file validated
SRR5030949 is single end
SRR5030949 is conventional basespace
SRR5030949 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.473	34.0	31.0	34.0	31.0	34.0
2	32.7925	34.0	31.0	34.0	31.0	34.0
3	32.78875	34.0	31.0	34.0	31.0	34.0
4	36.25025	37.0	37.0	37.0	35.0	37.0
5	36.30975	37.0	37.0	37.0	35.0	37.0
6	36.21875	37.0	37.0	37.0	35.0	37.0
7	36.225	37.0	36.0	37.0	35.0	37.0
8	36.262	37.0	37.0	37.0	35.0	37.0
9	38.0455	39.0	38.0	39.0	35.0	39.0
10	37.94475	39.0	38.0	39.0	35.0	39.0
11	37.9065	39.0	38.0	39.0	35.0	39.0
12	37.7325	39.0	38.0	39.0	35.0	39.0
13	37.82	39.0	38.0	39.0	35.0	39.0
14	39.2925	41.0	39.0	41.0	36.0	41.0
15	39.226	41.0	39.0	41.0	36.0	41.0
16	39.23225	41.0	39.0	41.0	36.0	41.0
17	39.30925	41.0	39.0	41.0	36.0	41.0
18	39.43075	41.0	39.0	41.0	36.0	41.0
19	39.3285	41.0	39.0	41.0	36.0	41.0
20	39.179	41.0	39.0	41.0	36.0	41.0
21	39.1685	41.0	39.0	41.0	36.0	41.0
22	39.31875	41.0	39.0	41.0	36.0	41.0
23	39.379	41.0	39.0	41.0	36.0	41.0
24	38.611	40.0	38.0	41.0	34.0	41.0
25	38.66175	40.0	38.0	41.0	34.0	41.0
26	38.89225	40.0	38.0	41.0	35.0	41.0
27	38.92125	40.0	39.0	41.0	35.0	41.0
28	38.685	40.0	38.0	41.0	34.0	41.0
29	38.0525	40.0	38.0	41.0	32.0	41.0
30	38.245	40.0	38.0	41.0	34.0	41.0
31	38.777	40.0	38.0	41.0	35.0	41.0
32	38.9195	40.0	38.0	41.0	35.0	41.0
33	37.78125	40.0	38.0	41.0	33.0	41.0
34	37.62975	40.0	37.0	41.0	31.0	41.0
35	37.51225	39.0	37.0	41.0	33.0	41.0
36	33.706	38.0	30.0	40.0	18.0	41.0
37	35.69425	38.0	34.0	40.0	30.0	41.0
38	37.0755	39.0	37.0	40.0	32.0	41.0
39	37.15	39.0	37.0	40.0	32.0	41.0
40	37.06875	39.0	37.0	40.0	32.0	41.0
41	36.803	39.0	36.0	40.0	31.0	41.0
42	36.89725	39.0	36.0	40.0	31.0	41.0
43	37.127	39.0	37.0	41.0	32.0	41.0
44	37.241	40.0	37.0	41.0	32.0	41.0
45	36.9855	39.0	36.0	41.0	31.0	41.0
46	34.53975	38.0	33.0	40.0	26.0	41.0
47	35.475	38.0	35.0	40.0	28.0	41.0
48	35.49725	38.0	35.0	40.0	29.0	41.0
49	34.7405	38.0	34.0	40.0	27.0	41.0
50	31.48975	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	2.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	1.0
20	7.0
21	0.0
22	10.0
23	10.0
24	11.0
25	15.0
26	18.0
27	27.0
28	30.0
29	49.0
30	45.0
31	60.0
32	93.0
33	108.0
34	147.0
35	209.0
36	288.0
37	543.0
38	1078.0
39	1246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.80701754385965	26.340852130325814	44.987468671679196	15.86466165413534
2	21.875	26.25	21.8	30.075000000000003
3	26.513256628314156	17.133566783391696	24.312156078039017	32.04102051025512
4	33.225	29.7	14.85	22.225
5	19.725	40.675	24.099999999999998	15.5
6	27.825	18.575	39.275	14.325
7	23.025000000000002	19.125	21.8	36.05
8	22.6	30.8	16.175	30.425
9	24.349999999999998	30.95	28.175	16.525000000000002
10	25.55	17.724999999999998	31.55	25.174999999999997
11	31.6	17.4	15.975	35.025
12	16.375	21.875	31.05	30.7
13	28.499999999999996	19.125	29.549999999999997	22.825
14	27.224999999999998	32.425	21.425	18.925
15	34.2	22.400000000000002	26.700000000000003	16.7
16	16.625	22.125	24.099999999999998	37.15
17	24.725	21.55	36.575	17.150000000000002
18	24.575	18.15	34.2	23.075000000000003
19	34.125	16.45	31.6	17.825
20	41.925000000000004	18.125	22.45	17.5
21	21.575	21.925	25.45	31.05
22	28.975	16.150000000000002	40.6	14.274999999999999
23	27.775	16.425	38.15	17.65
24	44.474999999999994	17.575	25.874999999999996	12.075
25	44.925	22.75	16.225	16.1
26	29.349999999999998	38.875	18.224999999999998	13.55
27	19.275000000000002	40.75	24.474999999999998	15.5
28	16.900000000000002	24.425	41.775	16.900000000000002
29	11.975	16.575	51.775000000000006	19.675
30	13.325000000000001	10.775	33.650000000000006	42.25
31	18.4	9.075	40.725	31.8
32	28.1	10.5	22.525000000000002	38.875
33	46.75	12.325	15.775	25.15
34	51.2	10.9	19.6	18.3
35	56.125	10.025	19.85	14.000000000000002
36	42.199999999999996	11.875	29.525000000000002	16.400000000000002
37	41.025	12.3	21.9	24.775
38	25.624999999999996	17.825	16.25	40.300000000000004
39	19.775000000000002	24.4	12.4	43.425000000000004
40	19.55	40.325	16.6	23.525
41	27.900000000000002	41.725	12.8	17.575
42	43.925	29.075	14.649999999999999	12.35
43	44.725	30.8	12.225	12.25
44	30.775000000000002	42.9	11.225	15.1
45	18.825	46.25	16.825000000000003	18.099999999999998
46	17.0	27.85	19.1	36.05
47	13.05	17.974999999999998	35.325	33.650000000000006
48	14.378594648662165	15.55388847211803	22.13053263315829	47.93698424606151
49	16.950000000000003	21.425	16.925	44.7
50	21.175	32.025	17.0	29.799999999999997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	1.0
22	2.0
23	1.5
24	1.0
25	0.5
26	0.0
27	2.5
28	5.0
29	9.0
30	13.0
31	24.5
32	36.0
33	36.0
34	36.0
35	48.5
36	61.0
37	75.0
38	89.0
39	98.0
40	107.0
41	87.5
42	68.0
43	113.5
44	159.0
45	204.5
46	250.0
47	307.5
48	365.0
49	432.0
50	499.0
51	612.5
52	726.0
53	545.0
54	364.0
55	433.5
56	503.0
57	401.5
58	300.0
59	188.5
60	77.0
61	59.5
62	42.0
63	57.0
64	72.0
65	54.0
66	36.0
67	24.5
68	13.0
69	24.5
70	36.0
71	75.0
72	114.0
73	64.5
74	15.0
75	8.5
76	2.0
77	1.0
78	0.0
79	1.0
80	2.0
81	3.0
82	4.0
83	2.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.05
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.025
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.925000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52882935352359	32.85
2	9.72626674432149	8.35
3	3.43622597553873	4.425
4	2.56260920209668	4.3999999999999995
5	1.6307513104251603	3.5000000000000004
6	0.5824111822947	1.5
7	0.6988934187536401	2.1
8	0.6988934187536401	2.4
9	0.46592894583576006	1.7999999999999998
>10	3.20326150262085	23.474999999999998
>50	0.34944670937682004	10.0
>100	0.11648223645894001	5.2
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	105	2.625	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	103	2.5749999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	94	2.35	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	69	1.725	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	63	1.575	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	62	1.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	57	1.425	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	55	1.375	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	34	0.8500000000000001	RNA PCR Primer, Index 2 (100% over 35bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	31	0.775	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	30	0.75	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	27	0.675	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	27	0.675	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	25	0.625	No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	24	0.6	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	23	0.575	RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	22	0.5499999999999999	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21	0.525	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	17	0.42500000000000004	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	17	0.42500000000000004	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	17	0.42500000000000004	No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	15	0.375	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	15	0.375	No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	14	0.35000000000000003	No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	14	0.35000000000000003	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	14	0.35000000000000003	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	12	0.3	RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	11	0.27499999999999997	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	11	0.27499999999999997	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	11	0.27499999999999997	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	10	0.25	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	9	0.22499999999999998	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	9	0.22499999999999998	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	9	0.22499999999999998	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	9	0.22499999999999998	No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	9	0.22499999999999998	RNA PCR Primer, Index 2 (100% over 44bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	8	0.2	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	8	0.2	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	8	0.2	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	8	0.2	RNA PCR Primer, Index 2 (100% over 50bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	8	0.2	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	8	0.2	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	8	0.2	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCATGATGAAGCCCATTATCAGTGGACATCTATGGATGAGAAATGCG	8	0.2	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGT	6	0.15	No Hit
GTCCATGATGAAGCCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	6	0.15	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	6	0.15	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	6	0.15	No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC	6	0.15	No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAAT	5	0.125	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	5	0.125	No Hit
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	5	0.125	RNA PCR Primer, Index 2 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	5	0.125	No Hit
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGTTTAACTTCTGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	5	0.125	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTAT	5	0.125	RNA PCR Primer, Index 2 (100% over 48bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGGGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (96% over 30bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTTAATGATGATTGTCTGTTTGATTGCTTGATGCAATGTGAAAACCACAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.225	0.0	0.0	0.0
2	0.0	0.25	0.0	0.0	0.0
3	0.0	0.375	0.0	0.0	0.0
4	0.0	0.4	0.0	0.0	0.0
5	0.0	0.45	0.0	0.0	0.0
6	0.0	0.55	0.0	0.0	0.0
7	0.0	0.875	0.0	0.0	0.0
8	0.0	0.925	0.0	0.0	0.0
9	0.0	0.95	0.0	0.0	0.0
10	0.0	1.075	0.0	0.0	0.0
11	0.0	1.25	0.0	0.0	0.0
12	0.0	1.425	0.0	0.0	0.0
13	0.0	1.575	0.0	0.0	0.0
14	0.0	1.825	0.0	0.0	0.0
15	0.0	2.1	0.0	0.0	0.0
16	0.0	3.55	0.0	0.0	0.0
17	0.0	4.0	0.0	0.0	0.0
18	0.0	5.375	0.0	0.0	0.0
19	0.0	5.775	0.0	0.0	0.0
20	0.0	6.525	0.0	0.0	0.0
21	0.0	11.775	0.0	0.0	0.0
22	0.0	20.725	0.0	0.0	0.0
23	0.0	42.5	0.0	0.0	0.0
24	0.0	53.35	0.0	0.0	0.0
25	0.0	58.475	0.0	0.0	0.0
26	0.0	60.1	0.0	0.0	0.0
27	0.0	61.1	0.0	0.0	0.0
28	0.0	61.725	0.0	0.0	0.0
29	0.0	62.3	0.0	0.0	0.0
30	0.0	62.75	0.0	0.0	0.0
31	0.0	63.425	0.0	0.0	0.0
32	0.0	64.575	0.0	0.0	0.0
33	0.0	65.075	0.0	0.0	0.0
34	0.0	67.15	0.0	0.0	0.0
35	0.0	68.2	0.0	0.0	0.0
36	0.0	69.275	0.0	0.0	0.0
37	0.0	70.125	0.0	0.0	0.0
38	0.0	70.675	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTTG	20	6.952051E-4	44.000004	18
GTTGAAG	20	6.952051E-4	44.000004	10
CAGTTGA	20	6.952051E-4	44.000004	8
CTGAGCT	40	5.315087E-9	44.000004	11
TAAACAT	20	6.952051E-4	44.000004	3
CCAGTTG	20	6.952051E-4	44.000004	7
AGTAGTT	20	6.952051E-4	44.000004	7
AAACATC	20	6.952051E-4	44.000004	4
AACTGTT	20	6.952051E-4	44.000004	17
GAGCTTG	40	5.315087E-9	44.000004	13
GGATCCG	40	5.315087E-9	44.000004	3
CCGTCTG	40	5.315087E-9	44.000004	7
TCGGATC	40	5.315087E-9	44.000004	1
TAGTTTG	20	6.952051E-4	44.000004	9
CGGATCC	40	5.315087E-9	44.000004	2
GAAGAAC	20	6.952051E-4	44.000004	13
GCCAGTT	20	6.952051E-4	44.000004	6
TAGTAGT	20	6.952051E-4	44.000004	6
GCTGCCA	20	6.952051E-4	44.000004	3
GAACTGT	20	6.952051E-4	44.000004	16
>>END_MODULE
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257436 spots for SRR5030949.sra
Written 257436 spots for SRR5030949.sra
Read 257437 spots for SRR5030949.sra
Written 257437 spots for SRR5030949.sra
SRR ids: ['SRR5030949.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_q2c4awpa
SRR5030949.sra spots: 3089233
blocks: [[1, 257436], [257437, 514872], [514873, 772308], [772309, 1029744], [1029745, 1287180], [1287181, 1544616], [1544617, 1802052], [1802053, 2059488], [2059489, 2316924], [2316925, 2574360], [2574361, 2831796], [2831797, 3089233]]
SRR5030949 file size 534356
SRR5030949 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030949 SRR5030949_1.fastq
Input file:	SRR5030949_1.fastq
trimmed:	SRR5030949-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:52:16 2020 >> started

Wed Mar  4 06:52:19 2020 >> done (3.122s)
3089233 reads processed; of these:
    172 ( 0.01%) short reads filtered out after trimming by size control
      4 ( 0.00%) empty reads filtered out after trimming by size control
3089057 (99.99%) reads available; of these:
 226468 ( 7.33%) trimmed reads available after processing
2862589 (92.67%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     54	  0.00%
 19	     40	  0.00%
 20	    146	  0.00%
 21	    159	  0.01%
 22	    267	  0.01%
 23	    342	  0.01%
 24	    402	  0.01%
 25	    442	  0.01%
 26	    441	  0.01%
 27	    522	  0.02%
 28	    650	  0.02%
 29	    819	  0.03%
 30	    730	  0.02%
 31	    993	  0.03%
 32	   1309	  0.04%
 33	   2554	  0.08%
 34	   6586	  0.21%
 35	   3972	  0.13%
 36	   2709	  0.09%
 37	   3124	  0.10%
 38	   4148	  0.13%
 39	   4255	  0.14%
 40	   5847	  0.19%
 41	   4480	  0.15%
 42	   7057	  0.23%
 43	   6351	  0.21%
 44	  15054	  0.49%
 45	  15566	  0.50%
 46	  15401	  0.50%
 47	  19655	  0.64%
 48	  43422	  1.41%
 49	  58971	  1.91%
 50	2862589	 92.67%
3089057 reads passed initial QC


criterion=sequence-density
sequence-density=70.07
sequence-density-rank=1
fanout-score=20.81
fanout-score-rank=2
prefix-density=72.89
prefix-fanout=20.0
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC


criterion=fanout-score
sequence-density=1.42
sequence-density-rank=7
fanout-score=164.09
fanout-score-rank=1
prefix-density=72.28
prefix-fanout=3.2
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC -o SRR5030949 -
Input file:	STDIN
trimmed:	SRR5030949-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:52:39 2020 >> started

Wed Mar  4 06:52:46 2020 >> done (7.069s)
3002041 reads processed; of these:
 146493 ( 4.88%) short reads filtered out after trimming by size control
   6049 ( 0.20%) empty reads filtered out after trimming by size control
2849499 (94.92%) reads available; of these:
2329673 (81.76%) trimmed reads available after processing
 519826 (18.24%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13651	  0.48%
 19	  22775	  0.80%
 20	 148452	  5.21%
 21	 265911	  9.33%
 22	 706381	 24.79%
 23	 305242	 10.71%
 24	 152855	  5.36%
 25	  43564	  1.53%
 26	  21922	  0.77%
 27	  17127	  0.60%
 28	  18871	  0.66%
 29	  14866	  0.52%
 30	  22317	  0.78%
 31	  34496	  1.21%
 32	  30761	  1.08%
 33	  65343	  2.29%
 34	  34376	  1.21%
 35	  28821	  1.01%
 36	  31822	  1.12%
 37	  20589	  0.72%
 38	  22556	  0.79%
 39	  23593	  0.83%
 40	  34645	  1.22%
 41	  44069	  1.55%
 42	  51410	  1.80%
 43	  38244	  1.34%
 44	  31066	  1.09%
 45	  48043	  1.69%
 46	  40619	  1.43%
 47	  29662	  1.04%
 48	   5387	  0.19%
 49	  15509	  0.54%
 50	 464554	 16.30%


criterion=sequence-density
sequence-density=6.01
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.70
sequence-density-rank=17
fanout-score=12.10
fanout-score-rank=1
prefix-density=8.49
prefix-fanout=1.0
sequence=TGAGCTTGGCTAT
                                 Started job on |	Mar 04 06:53:15
                             Started mapping on |	Mar 04 06:53:16
                                    Finished on |	Mar 04 06:53:39
       Mapping speed, Million of reads per hour |	459.63

                          Number of input reads |	2936515
                      Average input read length |	31
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1716870
                        Uniquely mapped reads % |	58.47%
                          Average mapped length |	29.71
                       Number of splices: Total |	1380
            Number of splices: Annotated (sjdb) |	261
                       Number of splices: GT/AG |	1239
                       Number of splices: GC/AG |	21
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	117
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.49
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	767444
             % of reads mapped to multiple loci |	26.13%
        Number of reads mapped to too many loci |	80785
             % of reads mapped to too many loci |	2.75%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.61%
                     % of reads unmapped: other |	1.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	452201	452201	452201
N_multimapping	767444	767444	767444
N_noFeature	85635	113917	1197879
N_ambiguous	912033	459364	22809
UnstrandedReadsAssigned:719202 PositiveStrandReadsAssigned:1143589 NegativeStrandReadsAssigned:496182
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030949 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030949-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,936,515 reads, 736,816 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,136 rounds

 109283 SRR5030949.ke.tsv
  52636 SRR5030949.se.tsv
 161919 total
==> SRR5030949.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030949.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030949 completed mapping pipeline successfully
