Starting /dee2/code/volunteer_pipeline.sh SRR5031085
    current disk space = 160492552192
    free memory = 73947008 
SRR5031085 SRAfilesize
de0780c7e5ecaf88288db79d93bb2e79  SRR5031085.sra
SRR5031085.sra file validated
SRR5031085 is single end
SRR5031085 is conventional basespace
SRR5031085 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5031085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.972	33.0	31.0	34.0	30.0	34.0
2	32.3845	33.0	31.0	34.0	31.0	34.0
3	32.26625	33.0	31.0	34.0	31.0	34.0
4	36.153	37.0	35.0	37.0	35.0	37.0
5	36.15775	37.0	35.0	37.0	35.0	37.0
6	35.9365	37.0	35.0	37.0	35.0	37.0
7	35.7705	37.0	35.0	37.0	35.0	37.0
8	35.5365	37.0	35.0	37.0	33.0	37.0
9	36.92175	39.0	37.0	39.0	33.0	39.0
10	36.4265	39.0	35.0	39.0	32.0	39.0
11	37.28	39.0	37.0	39.0	34.0	39.0
12	37.5435	39.0	37.0	39.0	35.0	39.0
13	37.321	39.0	37.0	39.0	35.0	39.0
14	38.5475	40.0	38.0	41.0	34.0	41.0
15	37.5475	40.0	37.0	41.0	31.0	41.0
16	36.797	39.0	36.0	40.0	31.0	41.0
17	36.9275	39.0	36.0	41.0	31.0	41.0
18	38.174	40.0	37.0	41.0	34.0	41.0
19	38.69875	40.0	38.0	41.0	35.0	41.0
20	38.51125	40.0	38.0	41.0	35.0	41.0
21	38.61175	40.0	38.0	41.0	35.0	41.0
22	37.91825	40.0	37.0	41.0	32.0	41.0
23	38.08225	39.0	38.0	40.0	34.0	41.0
24	37.5985	39.0	37.0	41.0	32.0	41.0
25	38.31225	40.0	38.0	41.0	34.0	41.0
26	38.24675	40.0	38.0	41.0	34.0	41.0
27	38.26625	40.0	38.0	41.0	34.0	41.0
28	38.4	40.0	38.0	41.0	34.0	41.0
29	38.54625	40.0	38.0	41.0	34.0	41.0
30	38.82625	40.0	38.0	41.0	35.0	41.0
31	38.87675	40.0	38.0	41.0	35.0	41.0
32	38.9015	40.0	38.0	41.0	35.0	41.0
33	38.93025	40.0	39.0	41.0	35.0	41.0
34	38.5725	40.0	39.0	41.0	34.0	41.0
35	37.717	40.0	37.0	41.0	31.0	41.0
36	33.21675	35.0	30.0	40.0	23.0	41.0
37	34.4995	37.0	32.0	38.0	27.0	40.0
38	36.20225	38.0	35.0	39.0	30.0	40.0
39	36.73825	38.0	36.0	40.0	31.0	40.0
40	37.26775	39.0	37.0	40.0	33.0	41.0
41	37.57875	40.0	37.0	40.0	33.0	41.0
42	37.57125	40.0	37.0	41.0	33.0	41.0
43	37.574	40.0	37.0	41.0	33.0	41.0
44	36.60375	39.0	36.0	40.0	30.0	41.0
45	36.5325	39.0	36.0	40.0	31.0	41.0
46	36.82575	39.0	36.0	40.0	30.0	41.0
47	36.1195	38.0	35.0	40.0	29.0	41.0
48	37.2005	39.0	37.0	40.0	32.0	41.0
49	37.43175	40.0	37.0	41.0	33.0	41.0
50	35.5135	38.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	2.0
16	0.0
17	2.0
18	3.0
19	2.0
20	1.0
21	2.0
22	6.0
23	9.0
24	6.0
25	9.0
26	11.0
27	13.0
28	26.0
29	29.0
30	46.0
31	65.0
32	85.0
33	143.0
34	177.0
35	285.0
36	458.0
37	682.0
38	1113.0
39	822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	5.626406601650412	28.182045511377847	56.71417854463616	9.477369342335585
2	29.662077596996244	30.01251564455569	20.600750938673343	19.72465581977472
3	16.525000000000002	23.225	18.475	41.775
4	30.125	43.575	9.975000000000001	16.325
5	24.7	36.625	29.65	9.025
6	29.475	15.299999999999999	46.35	8.875
7	11.825	23.7	23.95	40.525
8	24.775	43.2	13.05	18.975
9	15.2	39.324999999999996	33.7	11.774999999999999
10	15.4	21.8	25.7	37.1
11	41.699999999999996	7.1499999999999995	12.475	38.675
12	15.55	17.4	23.875	43.175000000000004
13	20.95	18.275	44.224999999999994	16.55
14	44.725	18.575	26.775	9.925
15	43.275000000000006	13.15	30.275000000000002	13.3
16	12.174999999999999	19.425	24.725	43.675000000000004
17	31.175000000000004	19.85	36.525	12.45
18	35.175	18.275	27.175	19.375
19	36.025	17.675	35.475	10.825
20	58.3	15.049999999999999	16.85	9.8
21	16.55	29.375	28.875	25.2
22	33.975	8.3	50.5	7.225
23	23.75	11.875	53.77499999999999	10.6
24	51.625	7.9750000000000005	32.45	7.95
25	61.150000000000006	19.45	13.775	5.625
26	33.550000000000004	49.3	10.2	6.950000000000001
27	13.075000000000001	58.15	21.0	7.775
28	8.525	30.65	52.1	8.725
29	8.649999999999999	8.575000000000001	63.87500000000001	18.9
30	7.449999999999999	3.6999999999999997	44.025	44.824999999999996
31	9.975000000000001	3.3000000000000003	45.375	41.349999999999994
32	22.675	5.375	26.724999999999998	45.225
33	56.574999999999996	4.75	10.35	28.325
34	76.02499999999999	4.825	8.95	10.2
35	72.35000000000001	6.9750000000000005	15.325	5.35
36	47.725	6.175	37.75	8.35
37	45.45	7.1499999999999995	25.525	21.875
38	27.025	11.675	10.225	51.075
39	12.525	20.4	6.325	60.75000000000001
40	10.075000000000001	52.075	5.45	32.4
41	21.3	60.099999999999994	5.575	13.025
42	51.349999999999994	35.6	4.725	8.325000000000001
43	62.125	26.375	4.95	6.550000000000001
44	33.6	50.7	5.050000000000001	10.65
45	13.825000000000001	59.52499999999999	8.5	18.15
46	7.1	31.424999999999997	17.05	44.425
47	5.1499999999999995	13.25	37.925	43.675000000000004
48	6.1	9.275	26.125	58.5
49	10.075000000000001	19.075	10.05	60.8
50	16.975	40.325	8.25	34.449999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.5
32	1.0
33	3.5
34	6.0
35	7.5
36	9.0
37	7.5
38	6.0
39	10.5
40	15.0
41	19.5
42	24.0
43	95.5
44	167.0
45	213.5
46	260.0
47	426.0
48	592.0
49	589.0
50	586.0
51	874.0
52	1162.0
53	764.5
54	367.0
55	348.0
56	329.0
57	280.5
58	232.0
59	154.0
60	76.0
61	64.5
62	53.0
63	47.5
64	42.0
65	33.0
66	24.0
67	15.0
68	6.0
69	6.0
70	6.0
71	18.0
72	30.0
73	17.0
74	4.0
75	2.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13286713286713	27.575
2	9.37062937062937	6.7
3	2.5174825174825175	2.7
4	2.1678321678321675	3.1
5	2.1678321678321675	3.875
6	1.3286713286713288	2.85
7	0.6293706293706294	1.575
8	0.48951048951048953	1.4000000000000001
9	0.4195804195804196	1.35
>10	3.3566433566433567	25.424999999999997
>50	0.13986013986013987	3.075
>100	0.27972027972027974	20.375
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	314	7.85	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	233	5.825	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	153	3.8249999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	115	2.875	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	51	1.275	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	45	1.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC	41	1.0250000000000001	RNA PCR Primer, Index 9 (100% over 50bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	32	0.8	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	27	0.675	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	21	0.525	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT	18	0.44999999999999996	RNA PCR Primer, Index 9 (100% over 36bp)
AACATTCAACGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 33bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	14	0.35000000000000003	No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	14	0.35000000000000003	RNA PCR Primer, Index 7 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	14	0.35000000000000003	RNA PCR Primer, Index 7 (97% over 35bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGCCTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGTGGCATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 28bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	8	0.2	RNA PCR Primer, Index 6 (100% over 34bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTCTAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC	7	0.17500000000000002	RNA PCR Primer, Index 9 (100% over 44bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	7	0.17500000000000002	No Hit
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	6	0.15	No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGGGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (96% over 27bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TGAGGTAGTCGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGCCTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTCAAGTAATCCCGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTCTCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	5	0.125	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
AAGGGATTCTGATGTTGGTCACTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	5	0.125	No Hit
TAGCAGCACGTAAATATTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTCTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGCACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
AGCTCGCTCTGAAGGCCTGTTTCCTAGGCTTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	5	0.125	RNA PCR Primer, Index 9 (100% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.45	0.0	0.0	0.0
2	0.0	1.55	0.0	0.0	0.0
3	0.0	1.65	0.0	0.0	0.0
4	0.0	1.65	0.0	0.0	0.0
5	0.0	1.65	0.0	0.0	0.0
6	0.0	1.65	0.0	0.0	0.0
7	0.0	1.925	0.0	0.0	0.0
8	0.0	2.05	0.0	0.0	0.0
9	0.0	2.075	0.0	0.0	0.0
10	0.0	2.325	0.0	0.0	0.0
11	0.0	2.475	0.0	0.0	0.0
12	0.0	2.675	0.0	0.0	0.0
13	0.0	2.925	0.0	0.0	0.0
14	0.0	3.35	0.0	0.0	0.0
15	0.0	4.35	0.0	0.0	0.0
16	0.0	5.475	0.0	0.0	0.0
17	0.0	6.175	0.0	0.0	0.0
18	0.0	7.0	0.0	0.0	0.0
19	0.0	7.5	0.0	0.0	0.0
20	0.0	8.475	0.0	0.0	0.0
21	0.0	11.95	0.0	0.0	0.0
22	0.0	24.325	0.0	0.0	0.0
23	0.0	58.4	0.0	0.0	0.0
24	0.0	79.65	0.0	0.0	0.0
25	0.0	85.0	0.0	0.0	0.0
26	0.0	85.925	0.0	0.0	0.0
27	0.0	86.425	0.0	0.0	0.0
28	0.0	86.925	0.0	0.0	0.0
29	0.0	87.3	0.0	0.0	0.0
30	0.0	87.675	0.0	0.0	0.0
31	0.0	88.525	0.0	0.0	0.0
32	0.0	89.8	0.0	0.0	0.0
33	0.0	90.65	0.0	0.0	0.0
34	0.0	91.575	0.0	0.0	0.0
35	0.0	92.125	0.0	0.0	0.0
36	0.0	92.425	0.0	0.0	0.0
37	0.0	92.875	0.0	0.0	0.0
38	0.0	93.025	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGATT	20	6.952051E-4	44.000004	8
TGATGGA	20	6.952051E-4	44.000004	19
GGTGATG	20	6.952051E-4	44.000004	17
GTTTGTA	20	6.952051E-4	44.000004	11
CAGTTTG	20	6.952051E-4	44.000004	18
TACAGTT	20	6.952051E-4	44.000004	16
AGTAGTT	20	6.952051E-4	44.000004	7
TTGTACA	20	6.952051E-4	44.000004	13
AGTAGAT	20	6.952051E-4	44.000004	7
AGGATAG	45	2.728484E-10	44.000004	13
TCGGTGA	45	2.728484E-10	44.000004	15
ATAGGCT	40	5.315087E-9	44.000004	16
AGTGGAA	20	6.952051E-4	44.000004	21
TCGGATC	20	6.952051E-4	44.000004	1
CGGATCC	20	6.952051E-4	44.000004	2
GATTGTA	20	6.952051E-4	44.000004	11
ACATTCA	65	0.0	44.000004	2
GGATAGG	45	2.728484E-10	44.000004	14
ATAGTTT	20	6.952051E-4	44.000004	17
TAGTAGT	20	6.952051E-4	44.000004	6
>>END_MODULE
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
Read 162300 spots for SRR5031085.sra
Written 162300 spots for SRR5031085.sra
SRR ids: ['SRR5031085.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5lbx7586
SRR5031085.sra spots: 1947600
blocks: [[1, 162300], [162301, 324600], [324601, 486900], [486901, 649200], [649201, 811500], [811501, 973800], [973801, 1136100], [1136101, 1298400], [1298401, 1460700], [1460701, 1623000], [1623001, 1785300], [1785301, 1947600]]
SRR5031085 file size 336493
SRR5031085 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5031085 SRR5031085_1.fastq
Input file:	SRR5031085_1.fastq
trimmed:	SRR5031085-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 07:20:49 2020 >> started

Wed Mar  4 07:20:50 2020 >> done (1.454s)
1947600 reads processed; of these:
    139 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
1947455 (99.99%) reads available; of these:
 146504 ( 7.52%) trimmed reads available after processing
1800951 (92.48%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     39	  0.00%
 19	     67	  0.00%
 20	    112	  0.01%
 21	    184	  0.01%
 22	    297	  0.02%
 23	    327	  0.02%
 24	    313	  0.02%
 25	    467	  0.02%
 26	    359	  0.02%
 27	    434	  0.02%
 28	    447	  0.02%
 29	   1060	  0.05%
 30	    466	  0.02%
 31	    490	  0.03%
 32	    606	  0.03%
 33	   1359	  0.07%
 34	   2402	  0.12%
 35	   6541	  0.34%
 36	   3956	  0.20%
 37	   3131	  0.16%
 38	   2491	  0.13%
 39	   2766	  0.14%
 40	   3756	  0.19%
 41	   3929	  0.20%
 42	   4793	  0.25%
 43	   7582	  0.39%
 44	   7737	  0.40%
 45	   9988	  0.51%
 46	  10225	  0.53%
 47	  10532	  0.54%
 48	  30373	  1.56%
 49	  29275	  1.50%
 50	1800951	 92.48%
1947455 reads passed initial QC


criterion=sequence-density
sequence-density=93.68
sequence-density-rank=1
fanout-score=16.45
fanout-score-rank=2
prefix-density=93.09
prefix-fanout=16.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC


criterion=fanout-score
sequence-density=4.05
sequence-density-rank=2
fanout-score=64.01
fanout-score-rank=1
prefix-density=93.82
prefix-fanout=2.8
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC -o SRR5031085 -
Input file:	STDIN
trimmed:	SRR5031085-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 07:21:01 2020 >> started

Wed Mar  4 07:21:03 2020 >> done (2.126s)
1906020 reads processed; of these:
 117475 ( 6.16%) short reads filtered out after trimming by size control
  26882 ( 1.41%) empty reads filtered out after trimming by size control
1761663 (92.43%) reads available; of these:
1730992 (98.26%) trimmed reads available after processing
  30671 ( 1.74%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   9815	  0.56%
 19	  23795	  1.35%
 20	  91087	  5.17%
 21	 239595	 13.60%
 22	 657018	 37.30%
 23	 411297	 23.35%
 24	 122918	  6.98%
 25	  18026	  1.02%
 26	   9034	  0.51%
 27	   7822	  0.44%
 28	   8353	  0.47%
 29	   7418	  0.42%
 30	  12044	  0.68%
 31	  21262	  1.21%
 32	  15773	  0.90%
 33	  19352	  1.10%
 34	  10328	  0.59%
 35	   7026	  0.40%
 36	   7106	  0.40%
 37	   3657	  0.21%
 38	   3541	  0.20%
 39	   3058	  0.17%
 40	   3137	  0.18%
 41	   4450	  0.25%
 42	   4687	  0.27%
 43	   3638	  0.21%
 44	   2793	  0.16%
 45	   4042	  0.23%
 46	   2805	  0.16%
 47	   2049	  0.12%
 48	    351	  0.02%
 49	    380	  0.02%
 50	  24006	  1.36%


criterion=sequence-density
sequence-density=4.89
sequence-density-rank=1
fanout-score=2.56
fanout-score-rank=8
prefix-density=0.03
prefix-fanout=2.6
sequence=TGAGGTAGTAGATTGTATAGTT


criterion=fanout-score
sequence-density=2.25
sequence-density-rank=6
fanout-score=16.18
fanout-score-rank=1
prefix-density=2.25
prefix-fanout=16.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC
                                 Started job on |	Mar 04 07:21:21
                             Started mapping on |	Mar 04 07:21:22
                                    Finished on |	Mar 04 07:21:45
       Mapping speed, Million of reads per hour |	282.22

                          Number of input reads |	1803098
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	749843
                        Uniquely mapped reads % |	41.59%
                          Average mapped length |	22.92
                       Number of splices: Total |	2365
            Number of splices: Annotated (sjdb) |	323
                       Number of splices: GT/AG |	2298
                       Number of splices: GC/AG |	31
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	34
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.76
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	935831
             % of reads mapped to multiple loci |	51.90%
        Number of reads mapped to too many loci |	23462
             % of reads mapped to too many loci |	1.30%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.70%
                     % of reads unmapped: other |	0.51%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	117424	117424	117424
N_multimapping	935831	935831	935831
N_noFeature	51100	56313	564974
N_ambiguous	377906	213707	2723
UnstrandedReadsAssigned:320837 PositiveStrandReadsAssigned:479823 NegativeStrandReadsAssigned:182146
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR5031085 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5031085-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,803,098 reads, 271,748 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,008 rounds

 109283 SRR5031085.ke.tsv
  52636 SRR5031085.se.tsv
 161919 total
==> SRR5031085.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5031085.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5031085 completed mapping pipeline successfully
