Starting /dee2/code/volunteer_pipeline.sh SRR5030862
    current disk space = 160654934016
    free memory = 73952692 
SRR5030862 SRAfilesize
63acb2ea6d39b82fdf0f482e93fd1c59  SRR5030862.sra
SRR5030862.sra file validated
SRR5030862 is single end
SRR5030862 is conventional basespace
SRR5030862 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3685	33.0	31.0	34.0	31.0	34.0
2	32.71175	34.0	31.0	34.0	31.0	34.0
3	32.76425	34.0	31.0	34.0	31.0	34.0
4	36.245	37.0	37.0	37.0	35.0	37.0
5	36.144	37.0	37.0	37.0	35.0	37.0
6	35.96775	37.0	35.0	37.0	35.0	37.0
7	36.10975	37.0	35.0	37.0	35.0	37.0
8	36.14625	37.0	36.0	37.0	35.0	37.0
9	37.932	39.0	38.0	39.0	35.0	39.0
10	37.9	39.0	38.0	39.0	35.0	39.0
11	37.89525	39.0	38.0	39.0	35.0	39.0
12	37.88175	39.0	38.0	39.0	35.0	39.0
13	37.83575	39.0	38.0	39.0	35.0	39.0
14	39.1395	41.0	39.0	41.0	36.0	41.0
15	39.06425	40.0	38.0	41.0	36.0	41.0
16	39.08525	40.0	39.0	41.0	36.0	41.0
17	39.25125	41.0	39.0	41.0	36.0	41.0
18	39.13525	41.0	39.0	41.0	36.0	41.0
19	39.0005	40.0	39.0	41.0	35.0	41.0
20	39.12975	40.0	39.0	41.0	36.0	41.0
21	39.23575	41.0	39.0	41.0	36.0	41.0
22	39.07475	41.0	39.0	41.0	36.0	41.0
23	39.16	41.0	39.0	41.0	36.0	41.0
24	38.427	40.0	38.0	41.0	34.0	41.0
25	38.6775	40.0	38.0	41.0	34.0	41.0
26	38.6125	40.0	38.0	41.0	34.0	41.0
27	38.63975	40.0	38.0	41.0	34.0	41.0
28	38.65775	40.0	38.0	41.0	34.0	41.0
29	38.32325	40.0	38.0	41.0	33.0	41.0
30	38.15975	40.0	38.0	41.0	33.0	41.0
31	38.57825	40.0	38.0	41.0	34.0	41.0
32	38.71725	40.0	38.0	41.0	35.0	41.0
33	37.0875	40.0	37.0	41.0	30.0	41.0
34	36.6065	40.0	36.0	41.0	30.0	41.0
35	33.5105	38.0	32.0	40.0	16.0	41.0
36	30.467	37.0	24.0	40.0	8.0	41.0
37	30.5345	36.0	25.0	39.0	8.0	41.0
38	32.75	37.0	31.0	40.0	20.0	41.0
39	32.606	37.0	31.0	40.0	15.0	41.0
40	33.07475	38.0	32.0	40.0	15.0	41.0
41	31.29775	37.0	28.0	40.0	7.0	41.0
42	32.42175	37.0	31.0	40.0	2.0	41.0
43	31.1375	37.0	30.0	40.0	2.0	41.0
44	31.8245	37.0	31.0	40.0	2.0	41.0
45	30.17825	37.0	28.0	40.0	2.0	41.0
46	27.9475	35.0	21.0	39.0	2.0	40.0
47	27.86925	35.0	21.0	39.0	2.0	41.0
48	28.50925	35.0	24.0	39.0	2.0	41.0
49	26.61675	35.0	2.0	39.0	2.0	41.0
50	23.604	31.0	2.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	2.0
17	2.0
18	1.0
19	10.0
20	6.0
21	10.0
22	18.0
23	28.0
24	34.0
25	52.0
26	86.0
27	111.0
28	93.0
29	110.0
30	152.0
31	136.0
32	146.0
33	176.0
34	245.0
35	278.0
36	394.0
37	452.0
38	673.0
39	783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.527054108216433	24.32364729458918	43.812625250501	18.336673346693384
2	27.224999999999998	24.875	20.525	27.375
3	21.625	23.025000000000002	24.6	30.75
4	31.275	26.224999999999998	16.85	25.650000000000002
5	23.75	36.625	25.8	13.825000000000001
6	31.674999999999997	17.45	34.925	15.950000000000001
7	20.200000000000003	20.9	26.174999999999997	32.725
8	28.425	28.275	15.75	27.55
9	16.85	33.925	31.95	17.275
10	24.325	23.375	25.3	27.0
11	35.65	14.475	15.55	34.325
12	21.575	20.75	25.4	32.275
13	30.825000000000003	18.15	32.275	18.75
14	30.875000000000004	25.124999999999996	24.525	19.475
15	31.3	21.825	28.249999999999996	18.625
16	19.275000000000002	19.575	23.849999999999998	37.3
17	23.575	23.7	35.425000000000004	17.299999999999997
18	23.474999999999998	18.375	28.675	29.475
19	29.575000000000003	16.650000000000002	34.525	19.25
20	36.85	17.424999999999997	28.000000000000004	17.724999999999998
21	24.525	22.55	27.900000000000002	25.025
22	27.325	14.7	42.625	15.35
23	26.025	16.400000000000002	39.1	18.475
24	46.375	14.6	25.45	13.575000000000001
25	45.4	21.65	17.299999999999997	15.65
26	29.225	39.125	14.95	16.7
27	18.725	40.325	22.6	18.35
28	16.575	25.124999999999996	42.15	16.150000000000002
29	15.35	15.6	49.775000000000006	19.275000000000002
30	14.299999999999999	9.2	35.65	40.849999999999994
31	17.125	8.799999999999999	40.65	33.425
32	28.675	8.825	22.650000000000002	39.85
33	45.45	11.700000000000001	16.400000000000002	26.450000000000003
34	51.975	9.425	17.424999999999997	21.175
35	53.900000000000006	9.275	20.525	16.3
36	55.85	12.075	16.975	15.1
37	37.475	14.2	21.9	26.424999999999997
38	27.825	15.575	15.525	41.075
39	22.15	24.975	12.825000000000001	40.050000000000004
40	18.45	42.35	13.950000000000001	25.25
41	28.7	39.074999999999996	14.025000000000002	18.2
42	44.1	28.4	14.099999999999998	13.4
43	41.875	31.4	13.100000000000001	13.625000000000002
44	30.85	42.35	10.975	15.825
45	21.7	42.125	12.5	23.674999999999997
46	15.8	29.65	24.525	30.025000000000002
47	12.174999999999999	19.900000000000002	31.624999999999996	36.3
48	14.099999999999998	13.05	23.974999999999998	48.875
49	15.625	23.974999999999998	17.0	43.4
50	28.799999999999997	25.074999999999996	12.425	33.7
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	0.5
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.5
26	1.0
27	1.5
28	2.0
29	2.5
30	3.0
31	8.0
32	13.0
33	22.5
34	32.0
35	43.5
36	55.0
37	70.5
38	86.0
39	87.5
40	89.0
41	77.5
42	66.0
43	102.5
44	139.0
45	167.5
46	196.0
47	245.0
48	294.0
49	369.0
50	444.0
51	620.0
52	796.0
53	637.0
54	478.0
55	406.0
56	334.0
57	342.5
58	351.0
59	265.5
60	180.0
61	134.0
62	88.0
63	107.0
64	126.0
65	81.0
66	36.0
67	29.0
68	22.0
69	30.5
70	39.0
71	67.5
72	96.0
73	54.0
74	12.0
75	7.0
76	2.0
77	3.5
78	5.0
79	6.5
80	8.0
81	6.0
82	4.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11819519372241	41.349999999999994
2	8.582638548307994	8.75
3	2.550269740068661	3.9
4	1.2260912211868562	2.5
5	1.2260912211868562	3.125
6	0.9318293281020108	2.85
7	0.4413928396272682	1.575
8	0.5394801373222168	2.1999999999999997
9	0.392349190779794	1.7999999999999998
>10	2.8445316331535064	25.624999999999996
>50	0.0980872976949485	3.6999999999999997
>100	0.04904364884747425	2.625
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	105	2.625	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	91	2.275	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	57	1.425	No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	43	1.075	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC	36	0.8999999999999999	RNA PCR Primer, Index 2 (100% over 50bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	32	0.8	RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	26	0.65	RNA PCR Primer, Index 2 (100% over 35bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	26	0.65	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	26	0.65	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	25	0.625	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	23	0.575	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	23	0.575	No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (96% over 28bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	19	0.475	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG	19	0.475	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	19	0.475	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	17	0.42500000000000004	No Hit
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	17	0.42500000000000004	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	15	0.375	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	14	0.35000000000000003	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	14	0.35000000000000003	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	13	0.325	No Hit
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	13	0.325	RNA PCR Primer, Index 2 (100% over 44bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	12	0.3	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	12	0.3	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCG	11	0.27499999999999997	RNA PCR Primer, Index 1 (96% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT	11	0.27499999999999997	RNA PCR Primer, Index 2 (100% over 37bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCG	11	0.27499999999999997	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	10	0.25	RNA PCR Primer, Index 1 (100% over 32bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	10	0.25	No Hit
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	10	0.25	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCG	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	9	0.22499999999999998	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCG	9	0.22499999999999998	RNA PCR Primer, Index 1 (96% over 27bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	9	0.22499999999999998	No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	8	0.2	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	8	0.2	No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGC	8	0.2	RNA PCR Primer, Index 1 (96% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA	8	0.2	RNA PCR Primer, Index 2 (100% over 36bp)
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAC	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTAT	7	0.17500000000000002	RNA PCR Primer, Index 2 (100% over 48bp)
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAAT	7	0.17500000000000002	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	7	0.17500000000000002	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCCAGGAACTCCAGTCACCG	6	0.15	RNA PCR Primer, Index 2 (97% over 35bp)
TACCATGATGATAACATAGTTCAGCAGACTTAACTCTGATGAACAATCAT	6	0.15	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	6	0.15	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGGAATTCTCGGGTGCC	6	0.15	No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	6	0.15	RNA PCR Primer, Index 2 (100% over 34bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	6	0.15	RNA PCR Primer, Index 2 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAATTCCA	6	0.15	RNA PCR Primer, Index 1 (95% over 23bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGGGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (96% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	6	0.15	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	6	0.15	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATACT	6	0.15	No Hit
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	5	0.125	RNA PCR Primer, Index 2 (100% over 35bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGTGATGACATTATTTAGCGACCAAAGTCTGATAAAGATGATTGTGA	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	5	0.125	No Hit
TGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTGGAATTCT	5	0.125	No Hit
CGCTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
TCGGATCCGTCTGAGCTTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG	5	0.125	No Hit
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
CAGGAGGATCACGAGTTCGAGGCCAGCCTGGGCTACACATTTTTGGAATT	5	0.125	No Hit
CTGCGTTCGCGCTCTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGGGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (96% over 28bp)
CTTAATGATGATTGTCTGTTTGATTGCTTGATGCAATGTGAAAACCACAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.975	0.0	0.0	0.0
2	0.0	1.025	0.0	0.0	0.0
3	0.0	1.225	0.0	0.0	0.0
4	0.0	1.275	0.0	0.0	0.0
5	0.0	1.4	0.0	0.0	0.0
6	0.0	1.5	0.0	0.0	0.0
7	0.0	2.125	0.0	0.0	0.0
8	0.0	2.225	0.0	0.0	0.0
9	0.0	2.425	0.0	0.0	0.0
10	0.0	2.6	0.0	0.0	0.0
11	0.0	2.75	0.0	0.0	0.0
12	0.0	3.1	0.0	0.0	0.0
13	0.0	3.325	0.0	0.0	0.0
14	0.0	3.8	0.0	0.0	0.0
15	0.0	4.2	0.0	0.0	0.0
16	0.0	5.525	0.0	0.0	0.0
17	0.0	6.45	0.0	0.0	0.0
18	0.0	7.825	0.0	0.0	0.0
19	0.0	8.675	0.0	0.0	0.0
20	0.0	10.0	0.0	0.0	0.0
21	0.0	13.15	0.0	0.0	0.0
22	0.0	22.475	0.0	0.0	0.0
23	0.0	44.25	0.0	0.0	0.0
24	0.0	55.075	0.0	0.0	0.0
25	0.0	60.725	0.0	0.0	0.0
26	0.0	61.85	0.0	0.0	0.0
27	0.0	62.9	0.0	0.0	0.0
28	0.0	63.65	0.0	0.0	0.0
29	0.0	64.025	0.0	0.0	0.0
30	0.0	64.675	0.0	0.0	0.0
31	0.0	65.3	0.0	0.0	0.0
32	0.0	65.95	0.0	0.0	0.0
33	0.0	66.625	0.0	0.0	0.0
34	0.0	69.15	0.0	0.0	0.0
35	0.0	69.7	0.0	0.0	0.0
36	0.0	70.175	0.0	0.0	0.0
37	0.0	70.65	0.0	0.0	0.0
38	0.0	71.125	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGG	20	6.952051E-4	44.000004	18
AAGTAAT	20	6.952051E-4	44.000004	4
TCAAGTA	20	6.952051E-4	44.000004	2
TTCAAGT	20	6.952051E-4	44.000004	1
AGGATAG	20	6.952051E-4	44.000004	13
GGATAGG	20	6.952051E-4	44.000004	14
TCTTTTG	20	6.952051E-4	44.000004	17
CAGGATA	20	6.952051E-4	44.000004	12
GATAGGC	20	6.952051E-4	44.000004	15
CAAGTAA	20	6.952051E-4	44.000004	3
ACCGGTC	30	1.9515464E-6	44.0	10
AACGCTG	30	1.9515464E-6	44.0	8
ACGCTGT	30	1.9515464E-6	44.0	9
AACCGGT	30	1.9515464E-6	44.0	9
TTCAACG	30	1.9515464E-6	44.0	5
TCGGTGA	30	1.9515464E-6	44.0	15
GGTCTCT	30	1.9515464E-6	44.0	13
CTCTTTT	25	3.6958358E-5	44.0	16
ATAGGCT	25	3.6958358E-5	44.0	16
CATTCAA	30	1.9515464E-6	44.0	3
>>END_MODULE
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104403 spots for SRR5030862.sra
Written 104403 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
Read 104401 spots for SRR5030862.sra
Written 104401 spots for SRR5030862.sra
SRR ids: ['SRR5030862.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gcb83me5
SRR5030862.sra spots: 1252814
blocks: [[1, 104401], [104402, 208802], [208803, 313203], [313204, 417604], [417605, 522005], [522006, 626406], [626407, 730807], [730808, 835208], [835209, 939609], [939610, 1044010], [1044011, 1148411], [1148412, 1252814]]
SRR5030862 file size 216060
SRR5030862 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030862 SRR5030862_1.fastq
Input file:	SRR5030862_1.fastq
trimmed:	SRR5030862-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:30:59 2020 >> started

Wed Mar  4 06:31:00 2020 >> done (0.950s)
1252814 reads processed; of these:
     71 ( 0.01%) short reads filtered out after trimming by size control
      5 ( 0.00%) empty reads filtered out after trimming by size control
1252738 (99.99%) reads available; of these:
 139534 (11.14%) trimmed reads available after processing
1113204 (88.86%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     26	  0.00%
 19	     33	  0.00%
 20	     34	  0.00%
 21	     72	  0.01%
 22	    101	  0.01%
 23	    144	  0.01%
 24	    162	  0.01%
 25	    232	  0.02%
 26	    192	  0.02%
 27	    251	  0.02%
 28	    332	  0.03%
 29	    455	  0.04%
 30	    354	  0.03%
 31	    538	  0.04%
 32	   1273	  0.10%
 33	   4121	  0.33%
 34	   9390	  0.75%
 35	   2429	  0.19%
 36	   2481	  0.20%
 37	   1491	  0.12%
 38	   2970	  0.24%
 39	   3161	  0.25%
 40	   5038	  0.40%
 41	   3173	  0.25%
 42	   6432	  0.51%
 43	   3519	  0.28%
 44	  12180	  0.97%
 45	   9256	  0.74%
 46	   8813	  0.70%
 47	   9542	  0.76%
 48	  25267	  2.02%
 49	  26072	  2.08%
 50	1113204	 88.86%
1252738 reads passed initial QC


criterion=sequence-density
sequence-density=70.99
sequence-density-rank=1
fanout-score=21.34
fanout-score-rank=2
prefix-density=72.90
prefix-fanout=20.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC


criterion=fanout-score
sequence-density=2.93
sequence-density-rank=2
fanout-score=69.73
fanout-score-rank=1
prefix-density=71.93
prefix-fanout=2.8
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC -o SRR5030862 -
Input file:	STDIN
trimmed:	SRR5030862-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:31:10 2020 >> started

Wed Mar  4 06:31:12 2020 >> done (1.994s)
1217450 reads processed; of these:
  83077 ( 6.82%) short reads filtered out after trimming by size control
  13931 ( 1.14%) empty reads filtered out after trimming by size control
1120442 (92.03%) reads available; of these:
 896514 (80.01%) trimmed reads available after processing
 223928 (19.99%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   9098	  0.81%
 19	  11969	  1.07%
 20	  39162	  3.50%
 21	 110316	  9.85%
 22	 264431	 23.60%
 23	 139743	 12.47%
 24	  70459	  6.29%
 25	  18345	  1.64%
 26	  10660	  0.95%
 27	   8472	  0.76%
 28	   9170	  0.82%
 29	   8213	  0.73%
 30	   9409	  0.84%
 31	  11304	  1.01%
 32	  13348	  1.19%
 33	  32392	  2.89%
 34	  10512	  0.94%
 35	   7572	  0.68%
 36	   9219	  0.82%
 37	   6637	  0.59%
 38	   7142	  0.64%
 39	   7734	  0.69%
 40	  11599	  1.04%
 41	  15079	  1.35%
 42	  19171	  1.71%
 43	  13664	  1.22%
 44	  10681	  0.95%
 45	  14956	  1.33%
 46	  12315	  1.10%
 47	   9560	  0.85%
 48	   3034	  0.27%
 49	   5386	  0.48%
 50	 189690	 16.93%


criterion=sequence-density
sequence-density=6.42
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=13
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTT


criterion=fanout-score
sequence-density=2.70
sequence-density-rank=3
fanout-score=17.85
fanout-score-rank=1
prefix-density=2.74
prefix-fanout=17.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC
                                 Started job on |	Mar 04 06:31:34
                             Started mapping on |	Mar 04 06:31:34
                                    Finished on |	Mar 04 06:31:58
       Mapping speed, Million of reads per hour |	173.36

                          Number of input reads |	1155730
                      Average input read length |	31
                                    UNIQUE READS:
                   Uniquely mapped reads number |	575300
                        Uniquely mapped reads % |	49.78%
                          Average mapped length |	29.44
                       Number of splices: Total |	841
            Number of splices: Annotated (sjdb) |	143
                       Number of splices: GT/AG |	779
                       Number of splices: GC/AG |	17
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	45
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.33
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	351971
             % of reads mapped to multiple loci |	30.45%
        Number of reads mapped to too many loci |	27393
             % of reads mapped to too many loci |	2.37%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.69%
                     % of reads unmapped: other |	0.71%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	228459	228459	228459
N_multimapping	351971	351971	351971
N_noFeature	35121	43993	447130
N_ambiguous	294412	186673	7075
UnstrandedReadsAssigned:245767 PositiveStrandReadsAssigned:344634 NegativeStrandReadsAssigned:121095
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR5030862 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030862-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,155,730 reads, 229,430 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 976 rounds

 109283 SRR5030862.ke.tsv
  52636 SRR5030862.se.tsv
 161919 total
==> SRR5030862.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030862.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030862 completed mapping pipeline successfully
