Starting /dee2/code/volunteer_pipeline.sh SRR5030956
    current disk space = 160601522176
    free memory = 73927212 
SRR5030956 SRAfilesize
754147d9a97035e7f6771b088ceed320  SRR5030956.sra
SRR5030956.sra file validated
SRR5030956 is single end
SRR5030956 is conventional basespace
SRR5030956 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR5030956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4685	33.0	31.0	34.0	31.0	34.0
2	32.74075	34.0	31.0	34.0	31.0	34.0
3	32.74075	34.0	31.0	34.0	31.0	34.0
4	36.222	37.0	37.0	37.0	35.0	37.0
5	36.27875	37.0	37.0	37.0	35.0	37.0
6	36.075	37.0	36.0	37.0	35.0	37.0
7	36.12675	37.0	35.0	37.0	35.0	37.0
8	36.1745	37.0	37.0	37.0	35.0	37.0
9	37.94325	39.0	38.0	39.0	35.0	39.0
10	37.957	39.0	38.0	39.0	35.0	39.0
11	37.77425	39.0	38.0	39.0	35.0	39.0
12	37.76725	39.0	38.0	39.0	35.0	39.0
13	37.77675	39.0	38.0	39.0	35.0	39.0
14	39.19125	40.0	39.0	41.0	36.0	41.0
15	39.28625	41.0	39.0	41.0	36.0	41.0
16	39.30775	41.0	39.0	41.0	36.0	41.0
17	39.29675	41.0	39.0	41.0	36.0	41.0
18	39.37725	41.0	39.0	41.0	36.0	41.0
19	39.3405	41.0	39.0	41.0	36.0	41.0
20	39.311	41.0	39.0	41.0	36.0	41.0
21	39.22025	40.0	39.0	41.0	36.0	41.0
22	39.34025	41.0	39.0	41.0	36.0	41.0
23	39.336	41.0	39.0	41.0	36.0	41.0
24	38.5455	40.0	38.0	41.0	34.0	41.0
25	38.80925	40.0	39.0	41.0	35.0	41.0
26	38.94375	40.0	38.0	41.0	35.0	41.0
27	38.99	41.0	39.0	41.0	35.0	41.0
28	38.57525	40.0	38.0	41.0	34.0	41.0
29	38.03	40.0	38.0	41.0	32.0	41.0
30	38.047	40.0	38.0	41.0	33.0	41.0
31	38.57625	40.0	38.0	41.0	34.0	41.0
32	38.727	40.0	38.0	41.0	35.0	41.0
33	37.5215	40.0	38.0	41.0	32.0	41.0
34	37.556	40.0	37.0	41.0	31.0	41.0
35	37.31725	39.0	37.0	41.0	31.0	41.0
36	33.48925	38.0	30.0	40.0	18.0	41.0
37	35.69475	38.0	34.0	40.0	30.0	41.0
38	37.12475	39.0	37.0	40.0	32.0	41.0
39	37.134	39.0	37.0	40.0	32.0	41.0
40	37.09025	39.0	37.0	40.0	31.0	41.0
41	36.84725	39.0	36.0	40.0	31.0	41.0
42	36.697	39.0	36.0	40.0	30.0	41.0
43	36.88425	39.0	36.0	40.0	31.0	41.0
44	37.06425	39.0	37.0	41.0	32.0	41.0
45	36.63925	39.0	36.0	40.0	31.0	41.0
46	34.2795	38.0	33.0	40.0	24.0	41.0
47	35.186	38.0	34.0	40.0	27.0	41.0
48	35.2755	38.0	34.0	40.0	28.0	41.0
49	34.4795	38.0	34.0	40.0	26.0	41.0
50	30.78675	34.0	29.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	0.0
19	5.0
20	5.0
21	6.0
22	9.0
23	8.0
24	10.0
25	20.0
26	24.0
27	31.0
28	26.0
29	50.0
30	53.0
31	68.0
32	91.0
33	95.0
34	161.0
35	191.0
36	319.0
37	568.0
38	1087.0
39	1169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.78978978978979	25.625625625625624	41.391391391391394	18.193193193193196
2	22.925	25.275	21.675	30.125
3	24.562281140570285	18.55927963981991	23.08654327163582	33.79189594797399
4	31.0	29.5	15.4	24.099999999999998
5	19.675	40.45	23.75	16.125
6	27.900000000000002	18.05	39.800000000000004	14.249999999999998
7	23.875	18.525	23.549999999999997	34.050000000000004
8	23.549999999999997	29.325000000000003	16.5	30.625000000000004
9	25.174999999999997	29.975	29.425	15.425
10	28.175	17.925	31.225	22.675
11	33.35	19.1	15.325	32.225
12	16.425	24.075	30.225	29.275000000000002
13	27.400000000000002	19.75	29.075	23.775
14	25.1	31.525	21.9	21.475
15	32.5	23.400000000000002	27.200000000000003	16.900000000000002
16	17.95	23.625	23.925	34.5
17	26.35	22.075	34.8	16.775000000000002
18	22.7	19.900000000000002	35.3	22.1
19	34.475	17.2	29.75	18.575
20	39.0	19.6	24.15	17.25
21	24.175	20.599999999999998	24.075	31.15
22	28.4	16.675	40.775	14.149999999999999
23	26.450000000000003	16.475	38.15	18.925
24	44.6	18.45	23.974999999999998	12.975
25	43.05	21.7	17.675	17.575
26	27.500000000000004	38.9	17.25	16.35
27	19.8	40.475	23.150000000000002	16.575
28	16.8	24.725	40.75	17.724999999999998
29	14.099999999999998	17.525	48.675000000000004	19.7
30	13.575000000000001	13.175	30.9	42.35
31	18.0	10.424999999999999	42.05	29.525000000000002
32	27.950000000000003	12.1	21.925	38.025
33	46.025	14.325	16.3	23.35
34	50.0	12.025	19.8	18.175
35	53.2	12.174999999999999	19.625	15.0
36	40.45	13.425	31.1	15.024999999999999
37	40.725	14.174999999999999	21.2	23.9
38	24.85	18.0	16.8	40.35
39	20.200000000000003	24.2	13.225000000000001	42.375
40	19.275000000000002	40.300000000000004	17.599999999999998	22.825
41	26.025	40.65	15.575	17.75
42	44.3	27.1	15.075	13.525
43	43.325	29.9	13.900000000000002	12.875
44	29.5	43.8	12.1	14.6
45	20.424999999999997	46.1	16.25	17.224999999999998
46	17.025000000000002	27.950000000000003	21.224999999999998	33.800000000000004
47	14.149999999999999	18.125	36.35	31.374999999999996
48	16.400000000000002	14.975	21.275	47.349999999999994
49	17.974999999999998	21.45	17.575	43.0
50	20.825	33.725	17.025000000000002	28.425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.5
26	2.0
27	1.5
28	1.0
29	1.5
30	2.0
31	22.0
32	42.0
33	42.5
34	43.0
35	55.0
36	67.0
37	88.0
38	109.0
39	108.0
40	107.0
41	106.0
42	105.0
43	134.0
44	163.0
45	207.0
46	251.0
47	291.5
48	332.0
49	406.5
50	481.0
51	586.0
52	691.0
53	544.5
54	398.0
55	462.5
56	527.0
57	410.5
58	294.0
59	191.5
60	89.0
61	69.0
62	49.0
63	71.0
64	93.0
65	60.0
66	27.0
67	20.5
68	14.0
69	17.0
70	20.0
71	43.5
72	67.0
73	37.0
74	7.0
75	6.5
76	6.0
77	5.5
78	5.0
79	4.5
80	4.0
81	2.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.05
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.199999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53317535545023	31.874999999999996
2	9.537914691943127	8.05
3	3.909952606635071	4.95
4	2.1919431279620856	3.6999999999999997
5	1.7772511848341233	3.75
6	1.0071090047393365	2.55
7	0.8293838862559242	2.45
8	0.7109004739336493	2.4
9	0.35545023696682465	1.35
>10	3.7322274881516586	26.200000000000003
>50	0.35545023696682465	9.9
>100	0.05924170616113744	2.825
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	113	2.825	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	73	1.825	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	69	1.725	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	68	1.7000000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA	65	1.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
ACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGTC	50	1.25	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	42	1.05	No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGT	33	0.8250000000000001	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG	32	0.8	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 27bp)
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	24	0.6	No Hit
TCGGATCCGTCTGAGCTTGGCTATTGGAATTCTCGGGTGCCAAGGAACTC	24	0.6	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	23	0.575	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACAC	21	0.525	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	21	0.525	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC	20	0.5	RNA PCR Primer, Index 1 (100% over 26bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	19	0.475	RNA PCR Primer, Index 7 (97% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA	18	0.44999999999999996	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTCTGTGCTGAATGT	17	0.42500000000000004	No Hit
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC	17	0.42500000000000004	RNA PCR Primer, Index 9 (100% over 44bp)
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACTACATGATTTTCC	17	0.42500000000000004	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC	16	0.4	RNA PCR Primer, Index 9 (100% over 50bp)
TCGGATCCGTCTGAGCTTGGCTATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TCGGATCCGTCTGAGCTTGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAA	15	0.375	No Hit
GGTCCATGATGAAGCCCATTATCAGTGGACATCTATGGATGAGAAATGCG	15	0.375	No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT	14	0.35000000000000003	No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATGGA	14	0.35000000000000003	No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC	14	0.35000000000000003	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGGGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (96% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
CTGCAGTGATGACTATCTTAGGACACCTTTGGATTTACCGTGAAAAGAAG	13	0.325	No Hit
ATACATGATGATCTCACACAACTTGAACTCTCTCACTGATTACTTGATGA	13	0.325	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	12	0.3	No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATT	12	0.3	No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	12	0.3	RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA	11	0.27499999999999997	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGAGTCGATAACTGA	11	0.27499999999999997	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	11	0.27499999999999997	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TTGCAATGATGACTTATATATCTGTCAATCCCCTGAGAGATCACTGACGA	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATTGGAA	11	0.27499999999999997	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAT	11	0.27499999999999997	No Hit
GGTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCG	10	0.25	No Hit
GGAAATGATGATGCCTGTGACTGCTGACAAGACTCTGATGTCTGTTGTAT	10	0.25	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGC	10	0.25	No Hit
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGGGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	10	0.25	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT	10	0.25	No Hit
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTTGGAATTCTC	9	0.22499999999999998	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGACCCTGTTGATCTC	9	0.22499999999999998	No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
GTCCATGATGAAGCCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	9	0.22499999999999998	No Hit
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTAAGCCGCGCC	8	0.2	No Hit
TCGGATCCGTCTGAGCTTGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCACGCGGGAGGCCCGG	8	0.2	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGGGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (96% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT	8	0.2	No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AGTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAA	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 32bp)
TCGGATCCGTCTGAGCTTGGCAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGGAATTCTC	7	0.17500000000000002	No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA	7	0.17500000000000002	No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACTGCTAATGAAAACACAG	7	0.17500000000000002	No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCATTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTCCATGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	7	0.17500000000000002	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTCCTGTTGAAAAACCATAAT	7	0.17500000000000002	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGGGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (96% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTC	6	0.15	No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAG	6	0.15	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGATGACACCTGGGTATGCTGCACACTCCCGACTGCGTCGTGGGG	6	0.15	No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GCCCATGATGGTATGAGAGTAGTGGACAGAAGGGATTTCTGAAAAACACT	6	0.15	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTC	6	0.15	No Hit
GAGCAATGATGACCCCAACATGCCATCTGAGTGGCTTTGCTGAAATCCAG	6	0.15	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTGTCTCGCTGACGCCATCACCG	6	0.15	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCTAATGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTG	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT	5	0.125	RNA PCR Primer, Index 9 (100% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCT	5	0.125	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCCATGGAATTCTCGGGTG	5	0.125	No Hit
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
GCTGTACTGACTTGATGAAAGTACTTTTGAACCCTTTTCCATCTGATGTG	5	0.125	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA	5	0.125	No Hit
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA	5	0.125	No Hit
CGCACGGGGTCGGCGGCGATGTCGGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	5	0.125	RNA PCR Primer, Index 7 (97% over 35bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC	5	0.125	No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATCAAAC	5	0.125	No Hit
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.4	0.0	0.0	0.0
2	0.0	0.4	0.0	0.0	0.0
3	0.0	0.425	0.0	0.0	0.0
4	0.0	0.45	0.0	0.0	0.0
5	0.0	0.5	0.0	0.0	0.0
6	0.0	0.575	0.0	0.0	0.0
7	0.0	1.075	0.0	0.0	0.0
8	0.0	1.25	0.0	0.0	0.0
9	0.0	1.3	0.0	0.0	0.0
10	0.0	1.4	0.0	0.0	0.0
11	0.0	1.55	0.0	0.0	0.0
12	0.0	1.725	0.0	0.0	0.0
13	0.0	1.9	0.0	0.0	0.0
14	0.0	2.075	0.0	0.0	0.0
15	0.0	2.675	0.0	0.0	0.0
16	0.0	3.725	0.0	0.0	0.0
17	0.0	4.275	0.0	0.0	0.0
18	0.0	5.6	0.0	0.0	0.0
19	0.0	6.25	0.0	0.0	0.0
20	0.0	6.95	0.0	0.0	0.0
21	0.0	11.275	0.0	0.0	0.0
22	0.0	19.35	0.0	0.0	0.0
23	0.0	41.025	0.0	0.0	0.0
24	0.0	50.025	0.0	0.0	0.0
25	0.0	54.625	0.0	0.0	0.0
26	0.0	55.925	0.0	0.0	0.0
27	0.0	56.65	0.0	0.0	0.0
28	0.0	57.275	0.0	0.0	0.0
29	0.0	57.525	0.0	0.0	0.0
30	0.0	57.85	0.0	0.0	0.0
31	0.0	58.625	0.0	0.0	0.0
32	0.0	59.875	0.0	0.0	0.0
33	0.0	60.9	0.0	0.0	0.0
34	0.0	63.225	0.0	0.0	0.0
35	0.0	64.375	0.0	0.0	0.0
36	0.0	65.675	0.0	0.0	0.0
37	0.0	66.625	0.0	0.0	0.0
38	0.0	67.325	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCTG	20	6.952051E-4	44.000004	8
GGTGAGT	20	6.952051E-4	44.000004	17
ACGCTGT	20	6.952051E-4	44.000004	9
TTCAACG	20	6.952051E-4	44.000004	5
TCGGTGA	20	6.952051E-4	44.000004	15
CATTCAA	20	6.952051E-4	44.000004	3
TCAACGC	20	6.952051E-4	44.000004	6
ACATTCA	20	6.952051E-4	44.000004	2
GCTGTCG	20	6.952051E-4	44.000004	11
CGGTGAG	20	6.952051E-4	44.000004	16
CTGTCGG	20	6.952051E-4	44.000004	12
ATTCAAC	20	6.952051E-4	44.000004	4
GTCGGTG	20	6.952051E-4	44.000004	14
TGGCTTG	20	6.952051E-4	44.000004	18
TGTCGGT	20	6.952051E-4	44.000004	13
CAACGCT	20	6.952051E-4	44.000004	7
CGCTGTC	20	6.952051E-4	44.000004	10
CTGAGCT	35	1.02281774E-7	44.0	11
GAGCTTG	35	1.02281774E-7	44.0	13
GGATCCG	35	1.02281774E-7	44.0	3
>>END_MODULE
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
Read 184904 spots for SRR5030956.sra
Written 184904 spots for SRR5030956.sra
Read 184893 spots for SRR5030956.sra
Written 184893 spots for SRR5030956.sra
SRR ids: ['SRR5030956.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ivdqn2_2
SRR5030956.sra spots: 2218727
blocks: [[1, 184893], [184894, 369786], [369787, 554679], [554680, 739572], [739573, 924465], [924466, 1109358], [1109359, 1294251], [1294252, 1479144], [1479145, 1664037], [1664038, 1848930], [1848931, 2033823], [2033824, 2218727]]
SRR5030956 file size 383474
SRR5030956 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR5030956 SRR5030956_1.fastq
Input file:	SRR5030956_1.fastq
trimmed:	SRR5030956-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	12
Wed Mar  4 06:53:14 2020 >> started

Wed Mar  4 06:53:16 2020 >> done (1.692s)
2218727 reads processed; of these:
    127 ( 0.01%) short reads filtered out after trimming by size control
      6 ( 0.00%) empty reads filtered out after trimming by size control
2218594 (99.99%) reads available; of these:
 168817 ( 7.61%) trimmed reads available after processing
2049777 (92.39%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     33	  0.00%
 19	     36	  0.00%
 20	    114	  0.01%
 21	    123	  0.01%
 22	    251	  0.01%
 23	    271	  0.01%
 24	    315	  0.01%
 25	    356	  0.02%
 26	    328	  0.01%
 27	    449	  0.02%
 28	    560	  0.03%
 29	    780	  0.04%
 30	    637	  0.03%
 31	    817	  0.04%
 32	   1296	  0.06%
 33	   2023	  0.09%
 34	   5045	  0.23%
 35	   2940	  0.13%
 36	   1943	  0.09%
 37	   2417	  0.11%
 38	   3121	  0.14%
 39	   3210	  0.14%
 40	   4215	  0.19%
 41	   3487	  0.16%
 42	   5506	  0.25%
 43	   4840	  0.22%
 44	  11164	  0.50%
 45	  11829	  0.53%
 46	  11468	  0.52%
 47	  14335	  0.65%
 48	  30137	  1.36%
 49	  44771	  2.02%
 50	2049777	 92.39%
2218594 reads passed initial QC


criterion=sequence-density
sequence-density=67.09
sequence-density-rank=1
fanout-score=21.90
fanout-score-rank=2
prefix-density=70.52
prefix-fanout=20.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC


criterion=fanout-score
sequence-density=1.85
sequence-density-rank=5
fanout-score=124.24
fanout-score-rank=1
prefix-density=69.57
prefix-fanout=3.3
sequence=GAATTCTCGGGGGCCAAGGAACT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC -o SRR5030956 -
Input file:	STDIN
trimmed:	SRR5030956-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	12
Wed Mar  4 06:53:30 2020 >> started

Wed Mar  4 06:53:33 2020 >> done (3.023s)
2153341 reads processed; of these:
 109822 ( 5.10%) short reads filtered out after trimming by size control
   6348 ( 0.29%) empty reads filtered out after trimming by size control
2037171 (94.61%) reads available; of these:
1633397 (80.18%) trimmed reads available after processing
 403774 (19.82%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  13791	  0.68%
 19	  15366	  0.75%
 20	  95746	  4.70%
 21	 174009	  8.54%
 22	 459555	 22.56%
 23	 202877	  9.96%
 24	 104627	  5.14%
 25	  32701	  1.61%
 26	  16611	  0.82%
 27	  12305	  0.60%
 28	  14349	  0.70%
 29	  11568	  0.57%
 30	  17379	  0.85%
 31	  25517	  1.25%
 32	  25731	  1.26%
 33	  55552	  2.73%
 34	  27397	  1.34%
 35	  23651	  1.16%
 36	  25348	  1.24%
 37	  16359	  0.80%
 38	  18768	  0.92%
 39	  19641	  0.96%
 40	  30160	  1.48%
 41	  35573	  1.75%
 42	  40850	  2.01%
 43	  30437	  1.49%
 44	  25263	  1.24%
 45	  36911	  1.81%
 46	  30628	  1.50%
 47	  22406	  1.10%
 48	   4618	  0.23%
 49	  13879	  0.68%
 50	 357598	 17.55%


criterion=sequence-density
sequence-density=5.27
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=AACATTCAACGCTGTCGGTGAGTTTT


criterion=fanout-score
sequence-density=0.48
sequence-density-rank=26
fanout-score=17.09
fanout-score-rank=1
prefix-density=8.26
prefix-fanout=1.0
sequence=TGAGCTTGGCTTTT
                                 Started job on |	Mar 04 06:53:57
                             Started mapping on |	Mar 04 06:53:58
                                    Finished on |	Mar 04 06:54:21
       Mapping speed, Million of reads per hour |	329.08

                          Number of input reads |	2102424
                      Average input read length |	32
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1245530
                        Uniquely mapped reads % |	59.24%
                          Average mapped length |	30.89
                       Number of splices: Total |	1046
            Number of splices: Annotated (sjdb) |	210
                       Number of splices: GT/AG |	931
                       Number of splices: GC/AG |	20
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	95
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.38
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	522815
             % of reads mapped to multiple loci |	24.87%
        Number of reads mapped to too many loci |	75004
             % of reads mapped to too many loci |	3.57%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.07%
                     % of reads unmapped: other |	1.25%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	334079	334079	334079
N_multimapping	522815	522815	522815
N_noFeature	64073	87229	887485
N_ambiguous	651665	345532	18540
UnstrandedReadsAssigned:529792 PositiveStrandReadsAssigned:812769 NegativeStrandReadsAssigned:339505
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=22 echo kmer=19
SRR5030956 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR5030956-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,102,424 reads, 531,012 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,113 rounds

 109283 SRR5030956.ke.tsv
  52636 SRR5030956.se.tsv
 161919 total
==> SRR5030956.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR5030956.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR5030956 completed mapping pipeline successfully
