Starting /dee2/code/volunteer_pipeline.sh SRR13079628
    current disk space = 414468886528
    free memory = 73623996 
SRR13079628 SRAfilesize
5302fe404ad6b9f3db2d258a228a7acf  SRR13079628.sra
SRR13079628.sra file validated
SRR13079628 is paired end
SRR13079628 is conventional basespace
SRR13079628 read1 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20475	32.0	32.0	32.0	32.0	32.0
2	31.444	32.0	32.0	32.0	32.0	32.0
3	31.41775	32.0	32.0	32.0	32.0	32.0
4	31.536	32.0	32.0	32.0	32.0	32.0
5	31.3345	32.0	32.0	32.0	32.0	32.0
6	34.72175	36.0	36.0	36.0	32.0	36.0
7	34.559	36.0	36.0	36.0	32.0	36.0
8	34.63475	36.0	36.0	36.0	32.0	36.0
9	34.76475	36.0	36.0	36.0	32.0	36.0
10	34.7255	36.0	36.0	36.0	32.0	36.0
11	34.725	36.0	36.0	36.0	32.0	36.0
12	34.838	36.0	36.0	36.0	32.0	36.0
13	34.7275	36.0	36.0	36.0	32.0	36.0
14	34.85375	36.0	36.0	36.0	32.0	36.0
15	34.7305	36.0	36.0	36.0	32.0	36.0
16	34.7155	36.0	36.0	36.0	32.0	36.0
17	34.92275	36.0	36.0	36.0	32.0	36.0
18	34.91225	36.0	36.0	36.0	32.0	36.0
19	34.83425	36.0	36.0	36.0	32.0	36.0
20	34.76175	36.0	36.0	36.0	32.0	36.0
21	34.78575	36.0	36.0	36.0	32.0	36.0
22	34.759	36.0	36.0	36.0	32.0	36.0
23	34.65075	36.0	36.0	36.0	32.0	36.0
24	34.9615	36.0	36.0	36.0	32.0	36.0
25	34.63575	36.0	36.0	36.0	32.0	36.0
26	34.77825	36.0	36.0	36.0	32.0	36.0
27	34.62125	36.0	36.0	36.0	32.0	36.0
28	34.5425	36.0	36.0	36.0	32.0	36.0
29	34.6945	36.0	36.0	36.0	32.0	36.0
30	34.6535	36.0	36.0	36.0	32.0	36.0
31	34.5435	36.0	36.0	36.0	32.0	36.0
32	34.54625	36.0	36.0	36.0	32.0	36.0
33	34.578	36.0	36.0	36.0	32.0	36.0
34	34.59525	36.0	36.0	36.0	32.0	36.0
35	34.6545	36.0	36.0	36.0	32.0	36.0
36	34.546	36.0	36.0	36.0	32.0	36.0
37	34.536	36.0	36.0	36.0	32.0	36.0
38	34.4845	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	1.0
23	5.0
24	8.0
25	15.0
26	17.0
27	34.0
28	59.0
29	80.0
30	121.0
31	153.0
32	195.0
33	314.0
34	734.0
35	2262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.124999999999998	21.525	31.35	33.0
2	28.325	37.2	13.675	20.8
3	23.375	21.9	28.549999999999997	26.174999999999997
4	31.6	18.85	25.575	23.974999999999998
5	35.8	32.35	16.75	15.1
6	23.140495867768596	18.883045329326322	22.163786626596544	35.81267217630854
7	19.275000000000002	29.325000000000003	24.175	27.224999999999998
8	17.025000000000002	18.65	26.325	38.0
9	12.5	20.325	34.050000000000004	33.125
10	28.749999999999996	17.275	26.8	27.175
11	56.175	15.4	14.374999999999998	14.05
12	25.924999999999997	29.125	31.775	13.175
13	33.475	15.049999999999999	26.625	24.85
14	27.6	17.424999999999997	25.224999999999998	29.75
15	23.849999999999998	28.375	23.625	24.15
16	42.775	17.125	22.025	18.075
17	30.425	27.375	21.575	20.625
18	19.2	23.025000000000002	21.099999999999998	36.675000000000004
19	26.05	26.25	18.175	29.525000000000002
20	27.075	14.099999999999998	46.0	12.825000000000001
21	38.525	11.899999999999999	24.675	24.9
22	26.5	20.424999999999997	29.925	23.150000000000002
23	21.875	16.675	37.824999999999996	23.625
24	49.925000000000004	9.15	21.6	19.325
25	45.625	24.375	18.25	11.75
26	31.525	30.125	14.249999999999998	24.099999999999998
27	24.575	34.675	18.85	21.9
28	14.899999999999999	27.025	39.475	18.6
29	32.525	16.325	37.875	13.275
30	16.0	11.175	32.1	40.725
31	16.325	10.775	41.55	31.35
32	24.85	19.325	24.025	31.8
33	33.5	23.724999999999998	20.424999999999997	22.35
34	46.925	16.925	22.8	13.350000000000001
35	57.574999999999996	8.25	23.849999999999998	10.325
36	42.575	14.799999999999999	32.125	10.5
37	40.475	19.575	25.874999999999996	14.075
38	24.775	21.85	15.049999999999999	38.324999999999996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	0.5
21	0.0
22	0.5
23	1.0
24	1.0
25	2.5
26	4.0
27	4.0
28	5.0
29	6.0
30	13.0
31	20.0
32	20.0
33	27.0
34	34.0
35	34.0
36	38.5
37	43.0
38	57.0
39	71.0
40	71.0
41	159.5
42	248.0
43	238.5
44	229.0
45	229.0
46	285.5
47	342.0
48	342.0
49	387.0
50	432.0
51	538.0
52	644.0
53	644.0
54	575.5
55	507.0
56	507.0
57	415.0
58	323.0
59	460.5
60	598.0
61	598.0
62	488.5
63	379.0
64	214.5
65	50.0
66	50.0
67	40.5
68	31.0
69	31.0
70	22.0
71	13.0
72	10.0
73	7.0
74	7.0
75	6.5
76	6.0
77	6.0
78	4.0
79	2.0
80	1.5
81	1.0
82	1.0
83	1.5
84	2.0
85	2.0
86	1.5
87	1.0
88	3.0
89	5.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.17500000000000002
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.35294117647058	33.95
2	6.731352334748332	5.55
3	3.1534263189812006	3.9
4	1.637355973317162	2.7
5	1.3947847180109159	2.875
6	0.667070952092177	1.6500000000000001
7	0.3638568829593693	1.05
8	0.4851425106124924	1.6
9	0.3638568829593693	1.35
>10	2.2437841115827775	16.525000000000002
>50	0.2425712553062462	7.5249999999999995
>100	0.3638568829593693	21.325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAAC	183	4.575	RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAA	168	4.2	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTC	157	3.925	RNA PCR Primer, Index 14 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGC	131	3.2750000000000004	No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGC	109	2.725	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA	105	2.625	No Hit
AAAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTG	92	2.3	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA	91	2.275	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTG	64	1.6	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTA	54	1.35	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGC	45	1.125	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACT	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 25bp)
ACTCAAACTGGGGGCTCTTTTTGGAATTCTCGGGTGCC	34	0.8500000000000001	No Hit
AAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTGC	32	0.8	No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGT	24	0.6	No Hit
CCTGCATGATGACGAACAAATACTGACTTGGAATTCTC	24	0.6	No Hit
CATCAAAGTGGAGGCCCTCTCTTTGGAATTCTCGGGTG	22	0.5499999999999999	No Hit
GGCAGAGGAGGGCTGTTCTTCCCTGGAATTCTCGGGTG	22	0.5499999999999999	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGT	21	0.525	No Hit
TCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGGG	21	0.525	No Hit
CATCAAAGTGGAGGCCCTCTCTTGGAATTCTCGGGTGC	21	0.525	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGC	17	0.42500000000000004	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGC	17	0.42500000000000004	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTGGAA	16	0.4	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGC	16	0.4	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGG	15	0.375	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGC	15	0.375	No Hit
AGTGCCGCAGAGTTTGTAGTGTTGGAATTCTCGGGTGC	15	0.375	No Hit
CTCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGG	15	0.375	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGT	15	0.375	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTTGGAATTCTC	14	0.35000000000000003	No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGC	14	0.35000000000000003	No Hit
GGCAGAGGAGGGCTGTTCTTCCTGGAATTCTCGGGTGC	14	0.35000000000000003	No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAA	13	0.325	RNA PCR Primer, Index 1 (100% over 23bp)
TCTTAGATCGATGTGGTGCTCCGGAGTTGGAATTCTCG	13	0.325	No Hit
TCTTAGATCGATGTGGTGCTCCGGAATGGAATTCTCGG	13	0.325	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT	13	0.325	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTG	13	0.325	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGG	12	0.3	No Hit
CTCTTAGATCGATGTGGTGCTCCGGAATGGAATTCTCG	11	0.27499999999999997	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTTGGA	11	0.27499999999999997	No Hit
AAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCC	10	0.25	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATG	10	0.25	No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTG	10	0.25	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTC	9	0.22499999999999998	No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
CTGCATGATGACGAACAAATACTGACTTGGAATTCTCG	9	0.22499999999999998	No Hit
TTGTGGCAACAATGGCACGGCAAGGGAGCTGTATTTTT	9	0.22499999999999998	No Hit
TTGTGGCAACAATGGCACGGCAAGGGGATCCAGTCCTT	9	0.22499999999999998	No Hit
TGAGGTTGGTGTACTGTGTGTGATGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGG	8	0.2	No Hit
ACTCAAACTGTGTGACATTTTTGGAATTCTCGGGTGCC	8	0.2	No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGT	8	0.2	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTGGAATTCTCGG	8	0.2	No Hit
GGCAGAGGAGGGCTGTTCTTCTGGAATTCTCGGGTGCC	8	0.2	No Hit
TATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTG	8	0.2	No Hit
AAGACAGATGTTCTGATCAAGGCCCGAGATTTTGGAAT	8	0.2	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAA	7	0.17500000000000002	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
AAATCTACCTGCCTCTGCCTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
CTCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGG	6	0.15	No Hit
TATCGTCCTTCTGCCCAGCGATCTTGGAATTCTCGGGT	6	0.15	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTGGAATTCTCGGGTG	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGG	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGC	6	0.15	No Hit
AAATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGC	6	0.15	No Hit
GCATGATGACGAACAAATACTGACTTGGAATTCTCGGG	6	0.15	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGAATGGAATTCTCGGGTG	6	0.15	No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTG	6	0.15	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGT	5	0.125	No Hit
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTG	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCC	5	0.125	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATC	5	0.125	No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGT	5	0.125	No Hit
ACTCAAACTGGGGGCTCTTTTGATGGAATTCTCGGGTG	5	0.125	No Hit
ATCGATGTGGTGCTCCGGATGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
AAGACAGATGTTCTGATCAAGGCCCGAGATTTTTGGAA	5	0.125	No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGG	5	0.125	No Hit
TGTCTTGCAGGCCGTCATGCTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TGCTGTGATGAGATGACTAAGTAGGAAGTGCCGTCAGA	5	0.125	No Hit
TGAGGGGCAGAGAGCGAGACTTTTTGGAATTCTCGGGT	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
GGGTGCGTACCTGAATCGCCCTCGTGAAGCCGCGGAAC	5	0.125	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTTGGAATTCT	5	0.125	No Hit
AGCAGTGATGACAGATTGGATCTGACTTGGAATTCTCG	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATT	5	0.125	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA	5	0.125	No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	4.075	0.0	0.0	0.0
2	0.0	4.1	0.0	0.0	0.0
3	0.0	4.125	0.0	0.0	0.0
4	0.0	4.15	0.0	0.0	0.0
5	0.0	4.275	0.0	0.0	0.0
6	0.0	4.325	0.0	0.0	0.0
7	0.0	4.45	0.0	0.0	0.0
8	0.0	4.475	0.0	0.0	0.0
9	0.0	4.6	0.0	0.0	0.0
10	0.0	4.75	0.0	0.0	0.0
11	0.0	5.05	0.0	0.0	0.0
12	0.0	5.4	0.0	0.0	0.0
13	0.0	5.925	0.0	0.0	0.0
14	0.0	7.3	0.0	0.0	0.0
15	0.0	12.45	0.0	0.0	0.0
16	0.0	13.275	0.0	0.0	0.0
17	0.0	13.925	0.0	0.0	0.0
18	0.0	14.625	0.0	0.0	0.0
19	0.0	15.575	0.0	0.0	0.0
20	0.0	16.175	0.0	0.0	0.0
21	0.0	17.625	0.0	0.0	0.0
22	0.0	22.775	0.0	0.0	0.0
23	0.0	39.75	0.0	0.0	0.0
24	0.0	50.25	0.0	0.0	0.0
25	0.0	55.15	0.0	0.0	0.0
26	0.0	57.675	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	40	8.843199E-8	32.0	1
TAGAACT	20	0.0033271667	32.0	12
CGCTGAT	20	0.0033271667	32.0	28
CGCTGAA	20	0.0033271667	32.0	28
TGCACTT	20	0.0033271667	32.0	4
GTAGAAC	20	0.0033271667	32.0	11
CAATGGT	20	0.0033271667	32.0	6
TTGCACT	20	0.0033271667	32.0	3
TGGCAAT	20	0.0033271667	32.0	3
CAGTCAC	35	1.2459041E-6	32.0	27
GTCACAG	35	1.2459041E-6	32.0	29
ACTTGTC	20	0.0033271667	32.0	7
TGGTAGA	20	0.0033271667	32.0	9
GCGACCT	35	1.2459041E-6	32.0	2
GCGACCC	40	8.843199E-8	32.0	22
CCAGTCA	35	1.2459041E-6	32.0	26
GGCAATG	20	0.0033271667	32.0	4
CCGCTGA	40	8.843199E-8	32.0	27
CTCCAGT	35	1.2459041E-6	32.0	24
ATGGTAG	20	0.0033271667	32.0	8
>>END_MODULE
SRR13079628 read2 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079628_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.051	32.0	32.0	32.0	32.0	32.0
2	30.95625	32.0	32.0	32.0	32.0	32.0
3	30.8545	32.0	32.0	32.0	32.0	32.0
4	30.97575	32.0	32.0	32.0	32.0	32.0
5	31.053	32.0	32.0	32.0	32.0	32.0
6	34.3625	36.0	36.0	36.0	32.0	36.0
7	34.31525	36.0	36.0	36.0	32.0	36.0
8	34.35025	36.0	36.0	36.0	32.0	36.0
9	34.23975	36.0	36.0	36.0	32.0	36.0
10	34.1035	36.0	36.0	36.0	32.0	36.0
11	34.245	36.0	36.0	36.0	32.0	36.0
12	34.26675	36.0	36.0	36.0	32.0	36.0
13	34.28275	36.0	36.0	36.0	32.0	36.0
14	34.2695	36.0	36.0	36.0	32.0	36.0
15	34.33825	36.0	36.0	36.0	32.0	36.0
16	34.28925	36.0	36.0	36.0	32.0	36.0
17	34.06525	36.0	36.0	36.0	32.0	36.0
18	33.78625	36.0	36.0	36.0	32.0	36.0
19	34.24225	36.0	36.0	36.0	32.0	36.0
20	34.16125	36.0	36.0	36.0	32.0	36.0
21	34.1995	36.0	36.0	36.0	32.0	36.0
22	34.06375	36.0	36.0	36.0	32.0	36.0
23	34.292	36.0	36.0	36.0	32.0	36.0
24	33.6625	36.0	36.0	36.0	27.0	36.0
25	33.8355	36.0	36.0	36.0	32.0	36.0
26	34.1465	36.0	36.0	36.0	32.0	36.0
27	34.2045	36.0	36.0	36.0	32.0	36.0
28	34.0545	36.0	36.0	36.0	32.0	36.0
29	34.1265	36.0	36.0	36.0	32.0	36.0
30	34.07925	36.0	36.0	36.0	32.0	36.0
31	33.685	36.0	36.0	36.0	32.0	36.0
32	33.92825	36.0	36.0	36.0	32.0	36.0
33	33.84375	36.0	36.0	36.0	32.0	36.0
34	34.12625	36.0	36.0	36.0	32.0	36.0
35	33.9315	36.0	36.0	36.0	32.0	36.0
36	34.00575	36.0	36.0	36.0	32.0	36.0
37	34.04225	36.0	36.0	36.0	32.0	36.0
38	33.28975	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	1.0
17	1.0
18	2.0
19	4.0
20	4.0
21	9.0
22	16.0
23	15.0
24	17.0
25	39.0
26	42.0
27	62.0
28	79.0
29	105.0
30	132.0
31	179.0
32	252.0
33	330.0
34	834.0
35	1869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.079519879969993	37.259314828707176	25.906476619154787	18.754688672168044
2	24.224999999999998	26.1	14.524999999999999	35.15
3	18.5	41.449999999999996	20.75	19.3
4	29.875	17.849999999999998	12.325	39.95
5	38.625	21.55	19.2	20.625
6	35.825	15.950000000000001	19.2	29.025000000000002
7	32.69952464348261	22.86715036277208	13.335001250938202	31.098323742807104
8	34.98498498498498	30.255255255255253	14.014014014014014	20.745745745745744
9	31.298473855391546	29.647235426569928	20.115086314736054	18.939204403302476
10	27.152152152152155	21.57157157157157	17.34234234234234	33.933933933933936
11	30.678687703481096	22.26396193338342	16.078136739293765	30.979213623841723
12	20.555833750625936	19.203805708562843	25.7386079118678	34.501752628943414
13	26.082603254067582	28.035043804755944	11.889862327909889	33.99249061326658
14	27.523165539694467	26.296018031555224	18.95817680941648	27.222639619333833
15	28.800400701227147	25.870272977710997	20.31054345103932	25.018782870022537
16	30.879038317054846	23.56624092161282	24.768344603055347	20.786376158276983
17	33.49198396793587	17.860721442885772	31.538076152304612	17.109218436873746
18	17.546933667083856	18.197747183979978	24.25531914893617	40.0
19	21.63786626596544	28.800400701227147	21.788129226145756	27.773603806661658
20	21.718436873747496	24.223446893787575	32.089178356713425	21.968937875751504
21	16.766766766766768	20.67067067067067	35.88588588588589	26.676676676676674
22	22.42242242242242	29.354354354354356	31.73173173173173	16.49149149149149
23	38.01652892561984	19.033308289506635	19.93488605058853	23.015276734285
24	21.371371371371374	40.31531531531532	19.344344344344343	18.96896896896897
25	20.305458187280923	27.616424636955433	31.021532298447674	21.056584877315974
26	23.71056584877316	19.42914371557336	25.01251877816725	31.84777165748623
27	47.069138276553105	13.877755511022045	19.564128256513026	19.488977955911825
28	32.197444249561514	11.074918566775244	36.933099473816085	19.794537709847155
29	31.64620395890754	13.831120020045102	26.45953395139063	28.063142069656728
30	40.66649962415435	11.826609872212478	23.12703583061889	24.379854673014282
31	43.61995487590875	23.514665329656555	14.514916019052393	18.350463775382302
32	31.621147582059635	30.66900526183914	13.179654221999499	24.53019293410173
33	25.13167795334838	22.447955856533735	17.682468021068473	34.73789816904941
34	27.819548872180448	13.283208020050125	35.91478696741854	22.982456140350877
35	34.429109159347554	16.58720200752823	25.59598494353827	23.387703889585946
36	24.398194583751255	20.661985957873622	42.07622868605817	12.863590772316952
37	27.962916562265093	28.36381859183162	24.830869456276623	18.84239538962666
38	38.46153846153847	30.568779754447505	15.45978451515911	15.509897268854925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	0.5
5	0.0
6	0.0
7	1.5
8	3.0
9	1.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.0
17	0.5
18	0.0
19	0.0
20	1.0
21	2.0
22	2.0
23	2.0
24	2.0
25	1.5
26	1.0
27	1.0
28	3.0
29	5.0
30	6.0
31	7.0
32	7.0
33	18.5
34	30.0
35	30.0
36	29.0
37	28.0
38	40.5
39	53.0
40	53.0
41	130.0
42	207.0
43	215.0
44	223.0
45	223.0
46	269.5
47	316.0
48	316.0
49	458.5
50	601.0
51	553.0
52	505.0
53	505.0
54	599.5
55	694.0
56	694.0
57	568.0
58	442.0
59	400.5
60	359.0
61	359.0
62	276.0
63	193.0
64	208.0
65	223.0
66	223.0
67	134.0
68	45.0
69	45.0
70	37.0
71	29.0
72	20.0
73	11.0
74	11.0
75	8.5
76	6.0
77	6.0
78	6.0
79	6.0
80	4.5
81	3.0
82	3.0
83	1.5
84	0.0
85	0.0
86	0.5
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.075
8	0.1
9	0.075
10	0.1
11	0.17500000000000002
12	0.15
13	0.125
14	0.17500000000000002
15	0.17500000000000002
16	0.17500000000000002
17	0.2
18	0.125
19	0.17500000000000002
20	0.2
21	0.1
22	0.1
23	0.17500000000000002
24	0.1
25	0.15
26	0.15
27	0.2
28	0.22499999999999998
29	0.22499999999999998
30	0.22499999999999998
31	0.27499999999999997
32	0.22499999999999998
33	0.325
34	0.25
35	0.375
36	0.3
37	0.22499999999999998
38	0.22499999999999998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.175000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0920671685003	35.875
2	6.77475390851187	5.8500000000000005
3	2.6635784597568035	3.45
4	1.7371163867979156	3.0
5	1.1001737116386796	2.375
6	0.6369426751592357	1.6500000000000001
7	0.4632310364794441	1.4000000000000001
8	0.5211349160393747	1.7999999999999998
9	0.23161551823972204	0.8999999999999999
>10	2.25825130283729	17.0
>50	0.23161551823972204	8.35
>100	0.2895193977996526	18.35
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGA	180	4.5	Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	166	4.15	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	146	3.65	Illumina RNA PCR Primer (100% over 33bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	129	3.225	No Hit
TCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	113	2.825	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	99	2.475	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATC	90	2.25	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTA	88	2.1999999999999997	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTA	57	1.425	No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAG	43	1.075	No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAA	39	0.975	Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGC	37	0.9249999999999999	No Hit
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGA	32	0.8	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAG	31	0.775	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGA	25	0.625	No Hit
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	24	0.6	No Hit
GGGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTA	21	0.525	No Hit
TCCGGAGCACCACATCGATCTAAGAGATCGTCGGACTG	21	0.525	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGT	20	0.5	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTA	19	0.475	No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAG	18	0.44999999999999996	No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAG	17	0.42500000000000004	No Hit
AGGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAG	17	0.42500000000000004	No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAG	16	0.4	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAG	16	0.4	No Hit
TTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	15	0.375	No Hit
GTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTA	15	0.375	No Hit
AAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATCG	15	0.375	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTG	15	0.375	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAG	15	0.375	No Hit
GGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAG	14	0.35000000000000003	No Hit
ACTTGGTTCCGCGGCTTCACGAGGGCGATTCAGGTACG	13	0.325	No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGAT	13	0.325	No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGA	13	0.325	No Hit
ACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGAC	13	0.325	No Hit
AACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGA	13	0.325	No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTG	13	0.325	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGGAGCACCACATCGATCTAAGAGGATCGTCGGACT	12	0.3	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAG	12	0.3	No Hit
AAAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATC	11	0.27499999999999997	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGT	11	0.27499999999999997	No Hit
TTCCGGAGCACCACATCGATCTAAGAGGATCGTCGGAC	11	0.27499999999999997	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGAC	10	0.25	No Hit
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	10	0.25	No Hit
AAAGTCTCGCTCTCTGCCCCTCAGATCGTCGGACTGTA	10	0.25	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTA	10	0.25	No Hit
TCACACACAGTACACCAACCTCAGATCGTCGGACTGTA	10	0.25	No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGA	10	0.25	No Hit
TCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCGA	9	0.22499999999999998	No Hit
AATCAGAACTTGACATTCAGCACAGAGTTTCTGTCCGC	9	0.22499999999999998	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGA	9	0.22499999999999998	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAG	9	0.22499999999999998	No Hit
AAAATACAGCTCCCTTGCCGTGCCATTGTTGCCACAAG	8	0.2	No Hit
GAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAGA	8	0.2	No Hit
AAATCTCGGGCCTTGATCAGAACATCTGTCTTGATCGT	8	0.2	No Hit
CATCAGATAGAGCTAATAAGATCTTCAGGTAGTCAGTA	8	0.2	No Hit
CCACGGGAACGGATCGTCGGACTGTAGAACTCTGAACG	8	0.2	Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGT	8	0.2	No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGT	8	0.2	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCAT	8	0.2	No Hit
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACT	8	0.2	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
CTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	7	0.17500000000000002	No Hit
AGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
TCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCGG	7	0.17500000000000002	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTG	7	0.17500000000000002	No Hit
CACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATCG	7	0.17500000000000002	No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGA	6	0.15	No Hit
AGATCGCTGGGCAGAAGGACGATAGATCGTCGGACTGT	6	0.15	No Hit
ATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG	6	0.15	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGT	6	0.15	No Hit
AAAATCTCGGGCCTTGATCAGAACATCTGTCTTGATCG	6	0.15	No Hit
AGTCAGTATTTGTTCGTCATCATGCGATCGTCGGACTG	6	0.15	No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTC	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGGATC	6	0.15	No Hit
TTCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	6	0.15	No Hit
ATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGAT	6	0.15	No Hit
TGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	6	0.15	No Hit
GAGTGTGACCAACATCAGAATCCCTTGATCGTCGGACT	5	0.125	No Hit
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTC	5	0.125	No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGG	5	0.125	No Hit
GGAGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAG	5	0.125	No Hit
ACTCAGAATCACCAGAAATCATCATTACTGATCGTCGG	5	0.125	No Hit
ACTGAGGGGCCTCAGACCGAGCTGATCGTCGGACTGTA	5	0.125	No Hit
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCG	5	0.125	No Hit
TCAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTA	5	0.125	No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGT	5	0.125	No Hit
AGTCAGCCAGGAGCAGTTATCGTCAGTTATCGACTCTG	5	0.125	No Hit
AAAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGT	5	0.125	No Hit
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	5	0.125	No Hit
CCGGAGCACCACATCGATCTAAGAGGATCGTCGGACTG	5	0.125	No Hit
ACCTGCACGAACAGCACTTTGGATCGTCGGACTGTAGA	5	0.125	No Hit
TCGGGCGCGTTCAGGGTGGTATGGCCGTAGACGATCGT	5	0.125	No Hit
TCCGGAGCACCACATCGATGATCGTCGGACTGTAGAAC	5	0.125	No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAA	5	0.125	No Hit
CAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGA	5	0.125	No Hit
GCTCAGCTCTCCAAGGTTGGCTTCCCCACGACGCAGTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.025	0.0	0.0
2	0.0	0.0	4.05	0.0	0.0
3	0.0	0.0	4.075	0.0	0.0
4	0.0	0.0	4.1	0.0	0.0
5	0.0	0.0	4.2	0.0	0.0
6	0.0	0.0	4.25	0.0	0.0
7	0.0	0.0	4.4	0.0	0.0
8	0.0	0.0	4.425	0.0	0.0
9	0.0	0.0	4.55	0.0	0.0
10	0.0	0.0	4.65	0.0	0.0
11	0.0	0.0	4.95	0.0	0.0
12	0.0	0.0	5.3	0.0	0.0
13	0.0	0.0	5.8	0.0	0.0
14	0.0	0.0	7.025	0.0	0.0
15	0.0	0.0	12.075	0.0	0.0
16	0.0	0.0	12.9	0.0	0.0
17	0.0	0.0	13.5	0.0	0.0
18	0.0	0.0	14.175	0.0	0.0
19	0.0	0.0	15.125	0.0	0.0
20	0.0	0.0	15.675	0.0	0.0
21	0.0	0.0	17.075	0.0	0.0
22	0.0	0.0	22.1	0.0	0.0
23	0.0	0.0	38.7	0.0	0.0
24	0.0	0.0	48.775	0.0	0.0
25	0.0	0.0	53.5	0.0	0.0
26	0.0	0.0	55.925	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGATC	35	1.2459041E-6	32.0	29
AACGTGT	35	1.2459041E-6	32.0	24
AAGTAGT	25	2.4147211E-4	32.0	9
TCCCAGG	20	0.0033271667	32.0	21
CCCAGGC	20	0.0033271667	32.0	22
AGTAGTA	25	2.4147211E-4	32.0	10
AGTAGCA	25	2.4147211E-4	32.0	13
GTGTAGA	35	1.2459041E-6	32.0	27
TAGCACT	25	2.4147211E-4	32.0	15
TTCCCAG	20	0.0033271667	32.0	20
AAAAGTA	25	2.4147211E-4	32.0	7
ACTCAAA	30	1.7408885E-5	32.0	3
TCAAAAG	25	2.4147211E-4	32.0	5
TAGTAGC	25	2.4147211E-4	32.0	12
TGTAGAT	35	1.2459041E-6	32.0	28
AGCACTT	30	1.7408885E-5	32.0	16
CGTGTAG	35	1.2459041E-6	32.0	26
GCACTTT	25	2.4147211E-4	32.0	17
AGACTCA	25	2.4147211E-4	32.0	1
ATTCCCA	20	0.0033271667	32.0	19
>>END_MODULE
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758131 spots for SRR13079628.sra
Written 758131 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
Read 758121 spots for SRR13079628.sra
Written 758121 spots for SRR13079628.sra
SRR ids: ['SRR13079628.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_j23tpkeu
SRR13079628.sra spots: 9097462
blocks: [[1, 758121], [758122, 1516242], [1516243, 2274363], [2274364, 3032484], [3032485, 3790605], [3790606, 4548726], [4548727, 5306847], [5306848, 6064968], [6064969, 6823089], [6823090, 7581210], [7581211, 8339331], [8339332, 9097462]]
SRR13079628 file size 1055055
SRR13079628 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR13079628 SRR13079628_1.fastq SRR13079628_2.fastq
Input file:	SRR13079628_1.fastq
Paired file:	SRR13079628_2.fastq
trimmed:	SRR13079628-trimmed-pair1.fastq, SRR13079628-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:26:17 2021 >> started

Tue Apr 20 16:26:25 2021 >> done (8.252s)
9097462 read pairs processed; of these:
   4583 ( 0.05%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
9092879 (99.95%) read pairs available; of these:
   5548 ( 0.06%) trimmed read pairs available after processing
9087331 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 27	      4	  0.00%
 28	    175	  0.00%
 29	    630	  0.01%
 30	    150	  0.00%
 31	    533	  0.01%
 32	    539	  0.01%
 33	    169	  0.00%
 34	    353	  0.00%
 35	    139	  0.00%
 36	    904	  0.01%
 37	   1952	  0.02%
 38	9087331	 99.94%
9092879 reads passed initial QC


criterion=sequence-density
sequence-density=59.62
sequence-density-rank=1
fanout-score=19.81
fanout-score-rank=1
prefix-density=61.91
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC


criterion=fanout-score
sequence-density=59.62
sequence-density-rank=1
fanout-score=19.81
fanout-score-rank=1
prefix-density=61.91
prefix-fanout=19.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC


criterion=sequence-density
sequence-density=58.03
sequence-density-rank=1
fanout-score=20.62
fanout-score-rank=1
prefix-density=60.43
prefix-fanout=19.8
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG


criterion=fanout-score
sequence-density=58.03
sequence-density-rank=1
fanout-score=20.62
fanout-score-rank=1
prefix-density=60.43
prefix-fanout=19.8
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC -y GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG -o SRR13079628 SRR13079628_1.fastq SRR13079628_2.fastq
Input file:	SRR13079628_1.fastq
Paired file:	SRR13079628_2.fastq
trimmed:	SRR13079628-trimmed-pair1.fastq, SRR13079628-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTTCC
-- paired 3' end adapter sequence (-y):	GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:27:15 2021 >> started

Tue Apr 20 16:27:23 2021 >> done (7.704s)
8789783 read pairs processed; of these:
1049357 (11.94%) short read pairs filtered out after trimming by size control
 364170 ( 4.14%) empty read pairs filtered out after trimming by size control
7376256 (83.92%) read pairs available; of these:
6364868 (86.29%) trimmed read pairs available after processing
1011388 (13.71%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  82625	  1.12%
 19	  68128	  0.92%
 20	 151343	  2.05%
 21	 446979	  6.06%
 22	1523920	 20.66%
 23	 866316	 11.74%
 24	 436266	  5.91%
 25	 270448	  3.67%
 26	 200474	  2.72%
 27	 163710	  2.22%
 28	 160502	  2.18%
 29	  92678	  1.26%
 30	  87230	  1.18%
 31	  93326	  1.27%
 32	 155737	  2.11%
 33	 545152	  7.39%
 34	 606281	  8.22%
 35	 149268	  2.02%
 36	 142515	  1.93%
 37	 123983	  1.68%
 38	1009375	 13.68%


criterion=sequence-density
sequence-density=8.43
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=TATTGCACTTGTCCCGGCCTGTAAA


criterion=fanout-score
sequence-density=0.39
sequence-density-rank=26
fanout-score=28.69
fanout-score-rank=1
prefix-density=9.29
prefix-fanout=1.2
sequence=GTCCCGGCCTGA


criterion=sequence-density
sequence-density=2.51
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=4
prefix-density=0.04
prefix-fanout=2.0
sequence=AGCCAAGCTCAGACGGATCCGA


criterion=fanout-score
sequence-density=0.34
sequence-density-rank=13
fanout-score=11.52
fanout-score-rank=1
prefix-density=0.30
prefix-fanout=11.5
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAG
SRR13079628 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:28:16
                             Started mapping on |	Apr 20 16:28:17
                                    Finished on |	Apr 20 16:28:59
       Mapping speed, Million of reads per hour |	658.23

                          Number of input reads |	7679352
                      Average input read length |	56
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4703839
                        Uniquely mapped reads % |	61.25%
                          Average mapped length |	50.01
                       Number of splices: Total |	15616
            Number of splices: Annotated (sjdb) |	8822
                       Number of splices: GT/AG |	13472
                       Number of splices: GC/AG |	593
                       Number of splices: AT/AC |	20
               Number of splices: Non-canonical |	1531
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.29
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	561136
             % of reads mapped to multiple loci |	7.31%
        Number of reads mapped to too many loci |	68469
             % of reads mapped to too many loci |	0.89%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	25.27%
                     % of reads unmapped: other |	5.27%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2414383	2414383	2414383
N_multimapping	561136	561136	561136
N_noFeature	681676	734660	3480562
N_ambiguous	2103142	1080133	196725
UnstrandedReadsAssigned:1919021 PositiveStrandReadsAssigned:2889046 NegativeStrandReadsAssigned:1026552
Dataset is classified unstranded
MeadianReadLen=25 20thPercentileLength=22 echo kmer=19
SRR13079628 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079628-trimmed-pair1.fastq
                             SRR13079628-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 7,679,352 reads, 1,949,716 reads pseudoaligned
[quant] estimated average fragment length: 29.8979
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,306 rounds

 109283 SRR13079628.ke.tsv
  52636 SRR13079628.se.tsv
 161919 total
==> SRR13079628.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079628.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079628 completed mapping pipeline successfully
