Starting /dee2/code/volunteer_pipeline.sh SRR13079634
    current disk space = 414752219136
    free memory = 1175476424 
SRR13079634 SRAfilesize
acab76bb2c42d7656647ecbe235402b3  SRR13079634.sra
SRR13079634.sra file validated
SRR13079634 is paired end
SRR13079634 is conventional basespace
SRR13079634 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9045	32.0	32.0	32.0	32.0	32.0
2	31.4205	32.0	32.0	32.0	32.0	32.0
3	31.514	32.0	32.0	32.0	32.0	32.0
4	31.5385	32.0	32.0	32.0	32.0	32.0
5	31.4245	32.0	32.0	32.0	32.0	32.0
6	35.099	36.0	36.0	36.0	36.0	36.0
7	35.06775	36.0	36.0	36.0	36.0	36.0
8	34.97375	36.0	36.0	36.0	36.0	36.0
9	34.966	36.0	36.0	36.0	36.0	36.0
10	35.01625	36.0	36.0	36.0	36.0	36.0
11	34.94275	36.0	36.0	36.0	36.0	36.0
12	35.0145	36.0	36.0	36.0	36.0	36.0
13	35.02575	36.0	36.0	36.0	36.0	36.0
14	34.93825	36.0	36.0	36.0	36.0	36.0
15	35.0095	36.0	36.0	36.0	36.0	36.0
16	34.99275	36.0	36.0	36.0	36.0	36.0
17	35.0235	36.0	36.0	36.0	36.0	36.0
18	35.0255	36.0	36.0	36.0	36.0	36.0
19	35.06975	36.0	36.0	36.0	36.0	36.0
20	35.04675	36.0	36.0	36.0	36.0	36.0
21	35.1165	36.0	36.0	36.0	36.0	36.0
22	35.1055	36.0	36.0	36.0	36.0	36.0
23	34.9315	36.0	36.0	36.0	36.0	36.0
24	34.952	36.0	36.0	36.0	36.0	36.0
25	34.93725	36.0	36.0	36.0	36.0	36.0
26	34.96075	36.0	36.0	36.0	36.0	36.0
27	34.91125	36.0	36.0	36.0	36.0	36.0
28	34.9625	36.0	36.0	36.0	32.0	36.0
29	35.04775	36.0	36.0	36.0	36.0	36.0
30	34.924	36.0	36.0	36.0	36.0	36.0
31	34.803	36.0	36.0	36.0	32.0	36.0
32	34.8575	36.0	36.0	36.0	32.0	36.0
33	34.88175	36.0	36.0	36.0	32.0	36.0
34	34.873	36.0	36.0	36.0	36.0	36.0
35	34.8505	36.0	36.0	36.0	36.0	36.0
36	34.78125	36.0	36.0	36.0	32.0	36.0
37	34.70825	36.0	36.0	36.0	32.0	36.0
38	34.82575	36.0	36.0	36.0	32.0	36.0
39	34.78225	36.0	36.0	36.0	32.0	36.0
40	34.92325	36.0	36.0	36.0	36.0	36.0
41	34.585	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	1.0
23	5.0
24	5.0
25	12.0
26	18.0
27	28.0
28	35.0
29	58.0
30	89.0
31	121.0
32	180.0
33	264.0
34	629.0
35	2553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.1	38.875	29.025000000000002	20.0
2	27.975	26.6	20.65	24.775
3	21.425	20.625	35.55	22.400000000000002
4	35.099999999999994	18.325	19.875	26.700000000000003
5	26.125	33.525	19.725	20.625
6	28.271203402551915	27.62071553665249	17.96347260445334	26.144608456342254
7	27.55	32.275	20.674999999999997	19.5
8	20.474999999999998	21.95	23.225	34.35
9	18.65	24.5	39.5	17.349999999999998
10	41.199999999999996	19.475	23.95	15.375
11	42.325	21.525	17.25	18.9
12	31.900000000000002	25.05	25.35	17.7
13	29.325000000000003	23.925	30.7	16.05
14	38.475	20.3	25.8	15.425
15	21.725	24.9	29.299999999999997	24.075
16	26.6	26.200000000000003	32.375	14.825
17	22.95	21.0	30.225	25.825
18	20.375	26.75	34.75	18.125
19	31.775	22.15	28.625	17.45
20	23.625	21.425	39.65	15.299999999999999
21	30.175	18.475	30.475	20.875
22	25.95	32.925	24.875	16.25
23	28.050000000000004	43.075	12.725	16.150000000000002
24	39.5	35.949999999999996	13.5	11.05
25	21.925	43.375	19.35	15.35
26	19.7	20.825	30.599999999999998	28.875
27	26.674999999999997	19.45	15.25	38.625
28	46.475	16.575	14.35	22.6
29	42.199999999999996	29.525000000000002	10.825	17.45
30	29.125	47.475	7.8	15.6
31	33.975	43.35	10.05	12.625
32	39.324999999999996	39.875	7.9	12.9
33	29.45	46.650000000000006	7.675	16.225
34	39.550000000000004	26.924999999999997	7.6	25.924999999999997
35	21.65	33.825	8.825	35.699999999999996
36	18.6	42.825	9.4	29.175
37	14.725	35.449999999999996	8.125	41.699999999999996
38	19.625	40.575	7.199999999999999	32.6
39	29.549999999999997	21.275	10.375	38.800000000000004
40	35.25	20.8	21.6	22.35
41	17.25	18.125	34.2	30.425
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	1.0
11	1.5
12	2.0
13	1.0
14	0.0
15	0.0
16	0.5
17	1.0
18	0.5
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	1.0
26	2.0
27	3.0
28	5.0
29	7.0
30	8.5
31	10.0
32	10.0
33	10.0
34	10.0
35	25.5
36	41.0
37	41.0
38	52.5
39	64.0
40	140.0
41	216.0
42	216.0
43	267.0
44	318.0
45	430.0
46	542.0
47	542.0
48	543.5
49	545.0
50	687.0
51	829.0
52	692.5
53	556.0
54	556.0
55	461.5
56	367.0
57	311.5
58	256.0
59	256.0
60	190.0
61	124.0
62	102.5
63	81.0
64	81.0
65	49.5
66	18.0
67	10.5
68	3.0
69	3.0
70	1.5
71	0.0
72	0.5
73	1.0
74	1.0
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.85
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81950774840475	45.975
2	7.793983591613491	8.55
3	2.552415679124886	4.2
4	1.276207839562443	2.8000000000000003
5	1.0027347310847767	2.75
6	0.7748404740200547	2.55
7	0.5013673655423884	1.925
8	0.18231540565177756	0.8
9	0.3646308113035551	1.7999999999999998
>10	1.504102096627165	17.299999999999997
>50	0.18231540565177756	7.2749999999999995
>100	0.04557885141294439	4.075
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACTCAAACTGGGGGCTCTTTTAGATCGGAAGAGCACACGTC	163	4.075	No Hit
AGTGCCGCAGAGTTTGTAGTGTAGATCGGAAGAGCACACGT	83	2.075	No Hit
ACTCAAACTGGGGGCTCTTTTGAGATCGGAAGAGCACACGT	76	1.9	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGT	74	1.8499999999999999	No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGT	58	1.4500000000000002	No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACAC	50	1.25	No Hit
ACTCAAACTGTGTGACATTTTAGATCGGAAGAGCACACGTC	47	1.175	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA	46	1.15	TruSeq Adapter, Index 18 (100% over 40bp)
AGTGCCGCAGAGTTTGTAGTGAGATCGGAAGAGCACACGTC	44	1.0999999999999999	No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTT	42	1.05	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGT	40	1.0	No Hit
TATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGT	37	0.9249999999999999	No Hit
AAAGTGCTTCCCTTTTGTGTGTAGATCGGAAGAGCACACGT	29	0.7250000000000001	No Hit
TTTGGCAATGGTAGAACTCACACCAGATCGGAAGAGCACAC	28	0.7000000000000001	No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT	21	0.525	No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTT	19	0.475	No Hit
ACTCAAACTGGGGGCTCTTTCAGATCGGAAGAGCACACGTC	18	0.44999999999999996	No Hit
AAGGGATTCTGATGTTGGTCACACTAGATCGGAAGAGCACA	17	0.42500000000000004	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA	16	0.4	No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGT	16	0.4	No Hit
AGTGCCGCAGAGTTTGTAGTGCAGATCGGAAGAGCACACGT	15	0.375	No Hit
ACTCAAACTGGGGGCTCTTTAGATCGGAAGAGCACACGTCT	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGTC	14	0.35000000000000003	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTAGATCGGAAGAGCACAC	14	0.35000000000000003	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGG	14	0.35000000000000003	No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGGCACTGGTAGAATTCACTGAGATCGGAAGAGCACACG	13	0.325	No Hit
TTTGGCAATGGTAGAACTCACACCGAGATCGGAAGAGCACA	13	0.325	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAG	12	0.3	No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACG	12	0.3	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAA	11	0.27499999999999997	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCG	11	0.27499999999999997	No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACAC	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGT	10	0.25	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATC	10	0.25	No Hit
AAGTGCCGCCAGGTTTTGAGTGTAGATCGGAAGAGCACACG	10	0.25	No Hit
ACTCAAACTGTGTGACATTTAGATCGGAAGAGCACACGTCT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGTGCTTCCACTTTGTGTGCAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
CATCAAAGTGGAGGCCCTCTCTTAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTA	9	0.22499999999999998	No Hit
ATGGCACTGGTAGAATTCACTGAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
AAAGTGCTACTACTTTTGAGTCTAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
TATGGCACTGGTAGAATTCACCAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
CCTGCATGATGACGAACAAATACTGACTAGATCGGAAGAGC	8	0.2	No Hit
AAGGGATTCTGATGTTGGTCACACTCAGATCGGAAGAGCAC	8	0.2	No Hit
AAGTGCTACTACTTTTGAGTCTAGATCGGAAGAGCACACGT	8	0.2	No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTC	8	0.2	No Hit
TGGAAGACTAGTGATTTTGTTGTAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
ACTCAAACTGGGGGCTCTTTTAAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
TGGAAGACTAGTGATTTTGTTGTCAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
TGACTGTGATGAATCTTACTCTGAGTCACAGATCGGAAGAG	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAG	7	0.17500000000000002	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAAT	7	0.17500000000000002	No Hit
AAAGTGCTACTACTTTTGAGTCAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGA	7	0.17500000000000002	No Hit
TATGCAAGGGCAAGCTCTCTTCAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
GCCTGCTGGGGTGGAACCTGGTAGATCGGAAGAGCACACGT	6	0.15	No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACAC	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAG	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACAAGATCGGAAGAGCACAC	6	0.15	No Hit
ACTCAAACTATGGGGGCACTTTAGATCGGAAGAGCACACGT	6	0.15	No Hit
AAGGGATTCTGATGTTGGTCAGATCGGAAGAGCACACGTCT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCAAAGTGGAGGCCCTCTCCAGATCGGAAGAGCACACGT	6	0.15	No Hit
TTTGGCACTAGCACATTTTTGCTAGATCGGAAGAGCACACG	6	0.15	No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGG	6	0.15	No Hit
CTCGTGAAGCCGCGGAACCAAGTAGATCGGAAGAGCACACG	6	0.15	No Hit
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGT	6	0.15	No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGT	6	0.15	No Hit
ACTCAAACTGGGGGCTCTTTTGTAGATCGGAAGAGCACACG	6	0.15	No Hit
TGCGTACCTGAATCGCCCTCGTGAAGCCGCGGAACCAAGTA	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAA	6	0.15	No Hit
CGGAACCAAGTAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTAGATCGGAAGAGCACACGT	5	0.125	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGA	5	0.125	No Hit
CATCAAAGTGGAGGCCCTCTCTAGATCGGAAGAGCACACGT	5	0.125	No Hit
AGACGATGATGACTGCTTTTCTGAGTTAGATCGGAAGAGCA	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACAAGATCGGAAGAGCACACGT	5	0.125	No Hit
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACG	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGT	5	0.125	No Hit
GTACCTGAATCGCCCTCGTGAAGCCGCGGAACCAAGTAGAT	5	0.125	No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGT	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	5	0.125	No Hit
AGTGCCGCAGAGTTTGTAGTAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGCATTGTACAGGGCTATGAAGATCGGAAGAGCACACG	5	0.125	No Hit
TGGTAGACTATGGAACGTAGGAGATCGGAAGAGCACACGTC	5	0.125	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCAAGATCGGAA	5	0.125	No Hit
AAACATTCGCGGTGCACTTCTTAGATCGGAAGAGCACACGT	5	0.125	No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG	5	0.125	No Hit
CAAAGTGCTCATAGTGCAGGTAGAGATCGGAAGAGCACACG	5	0.125	No Hit
CCTGCATGATGACGAACAAATACTGACCAGATCGGAAGAGC	5	0.125	No Hit
ACTCTGTGCTGAATGTCAAGTTCTGATTAGATCGGAAGAGC	5	0.125	No Hit
TGAAACATACACGGGAAACCTCTAGATCGGAAGAGCACACG	5	0.125	No Hit
AAAGTGCCGCCAGGTTTTGAGTGCAGATCGGAAGAGCACAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.35	0.0	0.0	0.0	0.0
2	1.4	0.0	0.0	0.0	0.0
3	1.4	0.0	0.0	0.0	0.0
4	1.4	0.0	0.0	0.0	0.0
5	1.425	0.0	0.0	0.0	0.0
6	1.45	0.0	0.0	0.0	0.0
7	1.525	0.0	0.0	0.0	0.0
8	1.65	0.0	0.0	0.0	0.0
9	2.0	0.0	0.0	0.0	0.0
10	2.85	0.0	0.0	0.0	0.0
11	3.625	0.0	0.0	0.0	0.0
12	4.475	0.0	0.0	0.0	0.0
13	5.475	0.0	0.0	0.0	0.0
14	7.075	0.0	0.0	0.0	0.0
15	8.7	0.0	0.0	0.0	0.0
16	9.85	0.0	0.0	0.0	0.0
17	11.35	0.0	0.0	0.0	0.0
18	12.55	0.0	0.0	0.0	0.0
19	14.0	0.0	0.0	0.0	0.0
20	15.325	0.0	0.0	0.0	0.0
21	18.65	0.0	0.0	0.0	0.0
22	31.15	0.0	0.0	0.0	0.0
23	52.85	0.0	0.0	0.0	0.0
24	59.75	0.0	0.0	0.0	0.0
25	65.7	0.0	0.0	0.0	0.0
26	67.8	0.0	0.0	0.0	0.0
27	69.125	0.0	0.0	0.0	0.0
28	70.5	0.0	0.0	0.0	0.0
29	72.225	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGTA	30	9.417061E-6	35.000004	12
TTGTAGT	30	9.417061E-6	35.000004	14
TGCCGCA	30	9.417061E-6	35.000004	3
CCGCAGA	30	9.417061E-6	35.000004	5
GAGTTTG	30	9.417061E-6	35.000004	10
CAGAGTT	30	9.417061E-6	35.000004	8
AGAGTTT	30	9.417061E-6	35.000004	9
AGTGCCG	30	9.417061E-6	35.000004	1
TGTAGTG	30	9.417061E-6	35.000004	15
GCAGAGT	30	9.417061E-6	35.000004	7
CGCAGAG	30	9.417061E-6	35.000004	6
GCCGCAG	30	9.417061E-6	35.000004	4
CTCAAAC	35	6.175269E-7	35.000004	2
GTGCCGC	30	9.417061E-6	35.000004	2
TAGAACT	20	0.0021434545	35.0	12
GTAGAAC	20	0.0021434545	35.0	11
CAATGGT	20	0.0021434545	35.0	6
AAACTGG	25	1.4254966E-4	35.0	5
TGGCAAT	20	0.0021434545	35.0	3
TAGTGTA	25	1.4254966E-4	35.0	17
>>END_MODULE
SRR13079634 read2 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079634_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.701	32.0	32.0	32.0	32.0	32.0
2	30.65325	32.0	32.0	32.0	32.0	32.0
3	30.62325	32.0	32.0	32.0	32.0	32.0
4	30.61	32.0	32.0	32.0	32.0	32.0
5	30.39125	32.0	32.0	32.0	21.0	32.0
6	34.036	36.0	36.0	36.0	32.0	36.0
7	33.8185	36.0	36.0	36.0	32.0	36.0
8	33.84625	36.0	36.0	36.0	32.0	36.0
9	33.8745	36.0	36.0	36.0	32.0	36.0
10	33.98925	36.0	36.0	36.0	32.0	36.0
11	33.9765	36.0	36.0	36.0	32.0	36.0
12	33.89475	36.0	36.0	36.0	32.0	36.0
13	34.125	36.0	36.0	36.0	32.0	36.0
14	33.92775	36.0	36.0	36.0	32.0	36.0
15	33.83775	36.0	36.0	36.0	32.0	36.0
16	33.9385	36.0	36.0	36.0	32.0	36.0
17	34.193	36.0	36.0	36.0	32.0	36.0
18	34.10775	36.0	36.0	36.0	32.0	36.0
19	33.97075	36.0	36.0	36.0	32.0	36.0
20	33.954	36.0	36.0	36.0	32.0	36.0
21	34.101	36.0	36.0	36.0	32.0	36.0
22	33.93525	36.0	36.0	36.0	32.0	36.0
23	34.23325	36.0	36.0	36.0	32.0	36.0
24	33.75725	36.0	36.0	36.0	32.0	36.0
25	34.0555	36.0	36.0	36.0	32.0	36.0
26	34.03325	36.0	36.0	36.0	32.0	36.0
27	33.915	36.0	36.0	36.0	32.0	36.0
28	33.91375	36.0	36.0	36.0	32.0	36.0
29	33.92025	36.0	36.0	36.0	32.0	36.0
30	33.82725	36.0	36.0	36.0	32.0	36.0
31	33.8525	36.0	36.0	36.0	32.0	36.0
32	34.15	36.0	36.0	36.0	32.0	36.0
33	34.093	36.0	36.0	36.0	32.0	36.0
34	33.8575	36.0	36.0	36.0	32.0	36.0
35	33.91125	36.0	36.0	36.0	32.0	36.0
36	33.80075	36.0	36.0	36.0	32.0	36.0
37	33.64025	36.0	36.0	36.0	27.0	36.0
38	33.7645	36.0	36.0	36.0	32.0	36.0
39	33.5785	36.0	36.0	36.0	21.0	36.0
40	33.39275	36.0	36.0	36.0	21.0	36.0
41	32.8105	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	5.0
16	8.0
17	7.0
18	11.0
19	9.0
20	5.0
21	14.0
22	13.0
23	29.0
24	25.0
25	49.0
26	48.0
27	57.0
28	77.0
29	116.0
30	135.0
31	172.0
32	241.0
33	340.0
34	790.0
35	1846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.900000000000002	49.1	13.775	17.224999999999998
2	25.45	34.625	8.15	31.775
3	15.0	38.875	23.025000000000002	23.1
4	20.349999999999998	32.725	16.025	30.9
5	25.374999999999996	37.175000000000004	20.75	16.7
6	29.75	27.675	15.625	26.950000000000003
7	20.349999999999998	30.425	22.375	26.85
8	22.1	29.7	17.974999999999998	30.225
9	17.5	28.349999999999998	19.3	34.849999999999994
10	15.15	25.374999999999996	27.1	32.375
11	25.775	20.599999999999998	19.900000000000002	33.725
12	19.3	18.125	24.95	37.625
13	23.400000000000002	33.225	15.625	27.750000000000004
14	27.825	23.849999999999998	24.95	23.375
15	25.0	19.625	28.125	27.250000000000004
16	24.95	22.85	30.75	21.45
17	27.1	19.625	31.424999999999997	21.85
18	31.225	20.325	24.05	24.4
19	25.2	26.5	19.1	29.2
20	26.625	26.900000000000002	25.924999999999997	20.549999999999997
21	21.725	18.75	33.375	26.150000000000002
22	26.924999999999997	24.75	31.35	16.975
23	40.849999999999994	25.75	17.925	15.475
24	20.025000000000002	43.925	22.3	13.750000000000002
25	20.125	18.525	33.225	28.125
26	25.7	17.925	22.2	34.175
27	37.5	14.025000000000002	30.95	17.525
28	22.775000000000002	12.525	37.475	27.224999999999998
29	34.48362090522631	13.453363340835208	17.35433858464616	34.70867716929232
30	49.35	16.725	17.95	15.975
31	39.825	28.65	13.750000000000002	17.775
32	25.674999999999997	32.75	18.175	23.400000000000002
33	21.975	23.775	22.025	32.225
34	29.725	17.375	36.675000000000004	16.225
35	32.425	18.075	30.9	18.6
36	21.525	23.9	42.0	12.575
37	31.05	34.625	18.25	16.075
38	40.725	32.475	16.8	10.0
39	20.9	49.675000000000004	12.075	17.349999999999998
40	19.45	43.7	14.924999999999999	21.925
41	21.6	23.425	22.775000000000002	32.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	3.0
4	3.5
5	4.0
6	2.5
7	1.0
8	1.0
9	1.0
10	1.0
11	2.0
12	3.0
13	2.5
14	2.0
15	2.0
16	1.5
17	1.0
18	1.5
19	2.0
20	2.0
21	1.0
22	0.0
23	2.0
24	4.0
25	4.0
26	5.0
27	6.0
28	7.5
29	9.0
30	11.5
31	14.0
32	14.0
33	15.0
34	16.0
35	21.5
36	27.0
37	27.0
38	41.0
39	55.0
40	146.5
41	238.0
42	238.0
43	268.0
44	298.0
45	452.0
46	606.0
47	606.0
48	696.0
49	786.0
50	787.0
51	788.0
52	571.0
53	354.0
54	354.0
55	309.0
56	264.0
57	227.5
58	191.0
59	191.0
60	165.5
61	140.0
62	116.0
63	92.0
64	92.0
65	63.0
66	34.0
67	27.5
68	21.0
69	21.0
70	13.5
71	6.0
72	5.0
73	4.0
74	4.0
75	4.0
76	4.0
77	3.0
78	2.0
79	2.5
80	3.0
81	3.0
82	3.0
83	3.0
84	1.5
85	0.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	2.0
94	1.0
95	0.0
96	1.5
97	3.0
98	3.0
99	6.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.025
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.81236203090508	48.025
2	7.0198675496688745	7.95
3	2.781456953642384	4.725
4	1.2803532008830023	2.9000000000000004
5	1.0154525386313467	2.875
6	0.5739514348785872	1.95
7	0.3090507726269316	1.225
8	0.44150110375275936	2.0
9	0.22075055187637968	1.125
>10	1.2803532008830023	14.124999999999998
>50	0.17660044150110377	6.7250000000000005
>100	0.08830022075055188	6.375
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	149	3.7249999999999996	No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	106	2.65	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAAC	78	1.95	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAAC	74	1.8499999999999999	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	61	1.525	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	56	1.4000000000000001	Illumina RNA PCR Primer (100% over 36bp)
AACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGA	49	1.225	No Hit
CACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAACT	43	1.075	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGAACT	42	1.05	No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAAC	39	0.975	No Hit
AGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAAC	38	0.95	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	28	0.7000000000000001	No Hit
ACACACAAAAGGGAAGCACTTTGATCGTCGGACTGTAGAAC	22	0.5499999999999999	No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAAC	20	0.5	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTAG	19	0.475	No Hit
GAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	18	0.44999999999999996	No Hit
GCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	18	0.44999999999999996	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTAGA	15	0.375	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAGAAC	15	0.375	No Hit
GCACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAAC	15	0.375	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	14	0.35000000000000003	No Hit
AAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACTC	14	0.35000000000000003	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGTCGG	14	0.35000000000000003	No Hit
AGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAGAACT	13	0.325	No Hit
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACG	13	0.325	Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTC	13	0.325	Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTC	13	0.325	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAA	12	0.3	No Hit
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAA	12	0.3	No Hit
CGCACGAGCCGAGTGATCCACCGCTAAGAGTCGATCGTCGG	12	0.3	No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATCGT	11	0.27499999999999997	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTAGAA	11	0.27499999999999997	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTAGAA	11	0.27499999999999997	No Hit
AGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	11	0.27499999999999997	No Hit
GGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	10	0.25	No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	9	0.22499999999999998	No Hit
GGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAAC	9	0.22499999999999998	No Hit
AGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAAC	9	0.22499999999999998	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAA	9	0.22499999999999998	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAGAA	9	0.22499999999999998	No Hit
ACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGAA	8	0.2	No Hit
CAGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAGAAC	8	0.2	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTATGATCGTC	8	0.2	No Hit
GAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	8	0.2	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8	0.2	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAGAAC	8	0.2	No Hit
AAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGAACTC	8	0.2	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCACACAAAGTGGAAGCACTTTGATCGTCGGACTGTAGAAC	8	0.2	No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACT	8	0.2	No Hit
ACTGAGGGGCCTCAGACCGAGCTGATCGTCGGACTGTAGAA	8	0.2	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCG	7	0.17500000000000002	No Hit
GCCTTCTGACTCCAAGTCCAGTGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
GACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
GTGACTCAGAGTAAGATTCATCACAGTCAGATCGTCGGACT	7	0.17500000000000002	No Hit
GAGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
ACTTGGTTCCGGATCGTCGGACTGTAGAACTCTGAACGTGT	7	0.17500000000000002	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
ACAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAA	6	0.15	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGACTG	6	0.15	No Hit
TAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAAC	6	0.15	No Hit
TCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAAC	6	0.15	No Hit
ACCAGGTTCCACCCCAGCAGGCGATCGTCGGACTGTAGAAC	6	0.15	No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAA	6	0.15	No Hit
AGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGA	6	0.15	No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAAC	6	0.15	No Hit
GGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAGAAC	6	0.15	No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGA	6	0.15	No Hit
AGCAAAAATGTGCTAGTGCCAAAGATCGTCGGACTGTAGAA	6	0.15	No Hit
ACTTGGTTCCGCGGCTTCACGAGGATCGTCGGACTGTAGAA	6	0.15	No Hit
GACCAACATCAGAATCCCTTGATCGTCGGACTGTAGAACTC	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAGAACT	5	0.125	No Hit
AAAGTGCCCCCATAGTTTGAGTGATCGTCGGACTGTAGAAC	5	0.125	No Hit
GCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAA	5	0.125	No Hit
TGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	5	0.125	No Hit
ACTTGGTTCCGCGGCTTCACGAGGGCGATTCAGGTACGATC	5	0.125	No Hit
AACTCAGAAAAGCAGTCATCATCGTCTGATCGTCGGACTGT	5	0.125	No Hit
CCTACGTTCCATAGTCTACCAGATCGTCGGACTGTAGAACT	5	0.125	No Hit
ACTTGGTTCCGCGGCTTCACGAGGGCGATTCAGGTACGCAG	5	0.125	No Hit
AATCAGAACTTGACATTCAGCACAGAGTGATCGTCGGACTG	5	0.125	No Hit
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
CTACCTGCACTATGAGCACTTTGGATCGTCGGACTGTAGAA	5	0.125	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGACTGT	5	0.125	No Hit
ACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAACTC	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGAC	5	0.125	No Hit
AGAGGTTTCCCGTGTATGTTTCAGATCGTCGGACTGTAGAA	5	0.125	No Hit
GAAGAGAGCTTGCCCTTGCATAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAGAAC	5	0.125	No Hit
GATCAGCAAGAGTATTCTCTTCATTTCAGGTCAGTCCCGAA	5	0.125	No Hit
TGCACGAGCCGAGTGATCCACCGCTAAGAGTCGATCGTCGG	5	0.125	No Hit
TGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGA	5	0.125	No Hit
CAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGG	5	0.125	No Hit
TACAGATGGATACCGTGCAATTGATCGTCGGACTGTAGAAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.425	0.0	0.0
2	0.0	0.0	1.45	0.0	0.0
3	0.0	0.0	1.45	0.0	0.0
4	0.0	0.0	1.45	0.0	0.0
5	0.0	0.0	1.45	0.0	0.0
6	0.0	0.0	1.475	0.0	0.0
7	0.0	0.0	1.525	0.0	0.0
8	0.0	0.0	1.675	0.0	0.0
9	0.0	0.0	2.075	0.0	0.0
10	0.0	0.0	2.925	0.0	0.0
11	0.0	0.0	3.675	0.0	0.0
12	0.0	0.0	4.5	0.0	0.0
13	0.0	0.0	5.55	0.0	0.0
14	0.0	0.0	7.125	0.0	0.0
15	0.0	0.0	8.825	0.0	0.0
16	0.0	0.0	10.0	0.0	0.0
17	0.0	0.0	11.425	0.0	0.0
18	0.0	0.0	12.575	0.0	0.0
19	0.0	0.0	13.925	0.0	0.0
20	0.0	0.0	15.25	0.0	0.0
21	0.0	0.0	18.525	0.0	0.0
22	0.0	0.0	30.75	0.0	0.0
23	0.0	0.0	51.95	0.0	0.0
24	0.0	0.0	58.725	0.0	0.0
25	0.0	0.0	64.625	0.0	0.0
26	0.0	0.0	66.775	0.0	0.0
27	0.0	0.0	68.0	0.0	0.0
28	0.0	0.0	69.325	0.0	0.0
29	0.0	0.0	70.9	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTCC	20	0.0021434545	35.0	4
ACTTGGT	20	0.0021434545	35.0	1
TTCCGCG	20	0.0021434545	35.0	7
GTTCCGC	20	0.0021434545	35.0	6
GCGGCTT	20	0.0021434545	35.0	11
ACACTAC	25	1.4254966E-4	35.0	1
GGTTCCG	25	1.4254966E-4	35.0	5
CTTGGTT	20	0.0021434545	35.0	2
AGTGTGA	20	0.0021434545	35.0	1
TTGGTTC	20	0.0021434545	35.0	3
CGCGGCT	20	0.0021434545	35.0	10
CGGCTTC	20	0.0021434545	35.0	12
TCCGCGG	20	0.0021434545	35.0	8
CCGCGGC	20	0.0021434545	35.0	9
TTTGAGT	35	2.707939E-5	30.000002	15
GAGTGAT	35	2.707939E-5	30.000002	18
TTGAGTG	35	2.707939E-5	30.000002	16
TGAGTGA	35	2.707939E-5	30.000002	17
CTCTGCG	30	4.1612564E-4	29.166668	11
CTACAAA	30	4.1612564E-4	29.166668	4
>>END_MODULE
Read 221743 spots for SRR13079634.sra
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SRR ids: ['SRR13079634.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_1c1k6gg_
SRR13079634.sra spots: 10643703
blocks: [[1, 221743], [221744, 443486], [443487, 665229], [665230, 886972], [886973, 1108715], [1108716, 1330458], [1330459, 1552201], [1552202, 1773944], [1773945, 1995687], [1995688, 2217430], [2217431, 2439173], [2439174, 2660916], [2660917, 2882659], [2882660, 3104402], [3104403, 3326145], [3326146, 3547888], [3547889, 3769631], [3769632, 3991374], [3991375, 4213117], [4213118, 4434860], [4434861, 4656603], [4656604, 4878346], [4878347, 5100089], [5100090, 5321832], [5321833, 5543575], [5543576, 5765318], [5765319, 5987061], [5987062, 6208804], [6208805, 6430547], [6430548, 6652290], [6652291, 6874033], [6874034, 7095776], [7095777, 7317519], [7317520, 7539262], [7539263, 7761005], [7761006, 7982748], [7982749, 8204491], [8204492, 8426234], [8426235, 8647977], [8647978, 8869720], [8869721, 9091463], [9091464, 9313206], [9313207, 9534949], [9534950, 9756692], [9756693, 9978435], [9978436, 10200178], [10200179, 10421921], [10421922, 10643703]]
SRR13079634 file size 1298368
SRR13079634 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 48 -o SRR13079634 SRR13079634_1.fastq SRR13079634_2.fastq
Input file:	SRR13079634_1.fastq
Paired file:	SRR13079634_2.fastq
trimmed:	SRR13079634-trimmed-pair1.fastq, SRR13079634-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:38:37 2021 >> started

Tue Apr 20 16:38:53 2021 >> done (15.940s)
10643703 read pairs processed; of these:
 1463544 (13.75%) short read pairs filtered out after trimming by size control
  231572 ( 2.18%) empty read pairs filtered out after trimming by size control
 8948587 (84.07%) read pairs available; of these:
 7905322 (88.34%) trimmed read pairs available after processing
 1043265 (11.66%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 155068	  1.73%
 19	 141846	  1.59%
 20	 379943	  4.25%
 21	1301745	 14.55%
 22	2313628	 25.85%
 23	 825488	  9.22%
 24	 606371	  6.78%
 25	 232817	  2.60%
 26	 133697	  1.49%
 27	 141328	  1.58%
 28	 163220	  1.82%
 29	 135025	  1.51%
 30	 130702	  1.46%
 31	 204486	  2.29%
 32	 228608	  2.55%
 33	 170523	  1.91%
 34	 167206	  1.87%
 35	 117401	  1.31%
 36	  89915	  1.00%
 37	  88582	  0.99%
 38	  66223	  0.74%
 39	  57358	  0.64%
 40	  54142	  0.61%
 41	1043265	 11.66%
8948587 reads passed initial QC


criterion=sequence-density
sequence-density=8.54
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=4
prefix-density=0.01
prefix-fanout=1.0
sequence=ACTCAAACTGGGGGCTCTTTTG


criterion=fanout-score
sequence-density=0.87
sequence-density-rank=11
fanout-score=42.55
fanout-score-rank=1
prefix-density=1.14
prefix-fanout=32.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCT


criterion=sequence-density
sequence-density=2.86
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=6
prefix-density=0.00
prefix-fanout=1.0
sequence=ACACTACAAACTCTGCGGCACT


criterion=fanout-score
sequence-density=0.82
sequence-density-rank=6
fanout-score=45.52
fanout-score-rank=1
prefix-density=1.00
prefix-fanout=37.1
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 48 -x ACTCAAACTGGGGGCTCTTTTG -y ACACTACAAACTCTGCGGCACT -o SRR13079634 SRR13079634_1.fastq SRR13079634_2.fastq
Input file:	SRR13079634_1.fastq
Paired file:	SRR13079634_2.fastq
trimmed:	SRR13079634-trimmed-pair1.fastq, SRR13079634-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACTCAAACTGGGGGCTCTTTTG
-- paired 3' end adapter sequence (-y):	ACACTACAAACTCTGCGGCACT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:40:30 2021 >> started

Tue Apr 20 16:40:44 2021 >> done (13.954s)
5965725 read pairs processed; of these:
   2035 ( 0.03%) short read pairs filtered out after trimming by size control
 795830 (13.34%) empty read pairs filtered out after trimming by size control
5167860 (86.63%) read pairs available; of these:
    138 ( 0.00%) trimmed read pairs available after processing
5167722 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 102817	  1.99%
 19	  92313	  1.79%
 20	 232102	  4.49%
 21	 502036	  9.71%
 22	1172281	 22.68%
 23	 527991	 10.22%
 24	 402824	  7.79%
 25	 153801	  2.98%
 26	  89038	  1.72%
 27	  93757	  1.81%
 28	 108614	  2.10%
 29	  89787	  1.74%
 30	  87107	  1.69%
 31	 136349	  2.64%
 32	 152304	  2.95%
 33	 113417	  2.19%
 34	 111783	  2.16%
 35	  78527	  1.52%
 36	  59818	  1.16%
 37	  59117	  1.14%
 38	  43847	  0.85%
 39	  38282	  0.74%
 40	  35924	  0.70%
 41	 684024	 13.24%


criterion=sequence-density
sequence-density=2.88
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=6
prefix-density=0.00
prefix-fanout=1.0
sequence=TTTGGCAATGGTAGAACTCACACCG


criterion=fanout-score
sequence-density=1.12
sequence-density-rank=10
fanout-score=48.48
fanout-score-rank=1
prefix-density=1.44
prefix-fanout=37.7
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCT


criterion=sequence-density
sequence-density=2.05
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=8
prefix-density=0.00
prefix-fanout=1.0
sequence=AGCCTATCCTGGATTACTTGAAGATCGTCGG


criterion=fanout-score
sequence-density=0.95
sequence-density-rank=5
fanout-score=50.84
fanout-score-rank=1
prefix-density=1.18
prefix-fanout=40.8
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC
SRR13079634 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:42:54
                             Started mapping on |	Apr 20 16:42:55
                                    Finished on |	Apr 20 16:43:52
       Mapping speed, Million of reads per hour |	514.78

                          Number of input reads |	8150722
                      Average input read length |	53
                                    UNIQUE READS:
                   Uniquely mapped reads number |	5283999
                        Uniquely mapped reads % |	64.83%
                          Average mapped length |	49.16
                       Number of splices: Total |	34666
            Number of splices: Annotated (sjdb) |	11697
                       Number of splices: GT/AG |	31205
                       Number of splices: GC/AG |	979
                       Number of splices: AT/AC |	29
               Number of splices: Non-canonical |	2453
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.25
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1253382
             % of reads mapped to multiple loci |	15.38%
        Number of reads mapped to too many loci |	98180
             % of reads mapped to too many loci |	1.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.92%
                     % of reads unmapped: other |	1.67%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1613341	1613341	1613341
N_multimapping	1253382	1253382	1253382
N_noFeature	962488	1115200	3756629
N_ambiguous	2107500	796674	397396
UnstrandedReadsAssigned:2214011 PositiveStrandReadsAssigned:3372125 NegativeStrandReadsAssigned:1129974
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13079634 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079634-trimmed-pair1.fastq
                             SRR13079634-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 8,150,722 reads, 1,913,780 reads pseudoaligned
[quant] estimated average fragment length: 27.6496
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,240 rounds

 109283 SRR13079634.ke.tsv
  52636 SRR13079634.se.tsv
 161919 total
==> SRR13079634.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079634.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079634 completed mapping pipeline successfully
