Starting /dee2/code/volunteer_pipeline.sh SRR13079630
    current disk space = 7156606588
    free memory = 132015524 
SRR13079630 SRAfilesize
83bb1ee1eaeb8d8ffd92ecb44be6cdbe  SRR13079630.sra
SRR13079630.sra file validated
SRR13079630 is paired end
SRR13079630 is conventional basespace
SRR13079630 read1 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079630_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00575	32.0	32.0	32.0	32.0	32.0
2	31.4695	32.0	32.0	32.0	32.0	32.0
3	31.33075	32.0	32.0	32.0	32.0	32.0
4	31.47925	32.0	32.0	32.0	32.0	32.0
5	31.4275	32.0	32.0	32.0	32.0	32.0
6	34.56675	36.0	36.0	36.0	32.0	36.0
7	34.26975	36.0	36.0	36.0	32.0	36.0
8	34.69375	36.0	36.0	36.0	32.0	36.0
9	34.664	36.0	36.0	36.0	32.0	36.0
10	34.5525	36.0	36.0	36.0	32.0	36.0
11	34.4395	36.0	36.0	36.0	32.0	36.0
12	34.67525	36.0	36.0	36.0	32.0	36.0
13	34.58675	36.0	36.0	36.0	32.0	36.0
14	34.649	36.0	36.0	36.0	32.0	36.0
15	34.67775	36.0	36.0	36.0	32.0	36.0
16	34.58825	36.0	36.0	36.0	32.0	36.0
17	34.7015	36.0	36.0	36.0	32.0	36.0
18	34.68325	36.0	36.0	36.0	32.0	36.0
19	34.6855	36.0	36.0	36.0	32.0	36.0
20	34.6915	36.0	36.0	36.0	32.0	36.0
21	34.71425	36.0	36.0	36.0	32.0	36.0
22	34.538	36.0	36.0	36.0	32.0	36.0
23	34.41025	36.0	36.0	36.0	32.0	36.0
24	34.4785	36.0	36.0	36.0	32.0	36.0
25	34.46775	36.0	36.0	36.0	32.0	36.0
26	34.44575	36.0	36.0	36.0	32.0	36.0
27	34.57475	36.0	36.0	36.0	32.0	36.0
28	34.46625	36.0	36.0	36.0	32.0	36.0
29	34.41775	36.0	36.0	36.0	32.0	36.0
30	34.54275	36.0	36.0	36.0	32.0	36.0
31	34.29325	36.0	36.0	36.0	32.0	36.0
32	33.98	36.0	36.0	36.0	32.0	36.0
33	34.0365	36.0	36.0	36.0	32.0	36.0
34	34.199	36.0	36.0	36.0	32.0	36.0
35	34.2205	36.0	36.0	36.0	32.0	36.0
36	34.26525	36.0	36.0	36.0	32.0	36.0
37	34.3825	36.0	36.0	36.0	32.0	36.0
38	34.1795	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	3.0
23	6.0
24	15.0
25	18.0
26	30.0
27	42.0
28	70.0
29	80.0
30	121.0
31	176.0
32	222.0
33	355.0
34	792.0
35	2068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.75	24.3	36.35	28.599999999999998
2	24.725	39.125	16.0	20.150000000000002
3	21.575	21.7	32.05	24.675
4	30.475	19.2	25.75	24.575
5	40.400000000000006	27.85	16.3	15.45
6	23.202204961162614	19.243297419193183	23.227261338010525	34.327236281633674
7	19.175	31.324999999999996	24.875	24.625
8	16.45	21.05	21.975	40.525
9	12.25	18.8	40.125	28.825
10	29.925	14.099999999999998	28.325	27.650000000000002
11	54.7	14.6	15.6	15.1
12	23.375	25.25	35.775	15.6
13	31.1	17.549999999999997	21.8	29.549999999999997
14	31.674999999999997	15.275	25.1	27.950000000000003
15	22.75	26.924999999999997	23.225	27.1
16	39.675	19.05	22.25	19.025
17	34.4	20.25	21.375	23.974999999999998
18	18.15	23.974999999999998	22.95	34.925
19	22.725	25.275	20.4	31.6
20	27.400000000000002	15.0	43.625	13.975000000000001
21	34.675	11.05	25.525	28.749999999999996
22	22.75	23.025000000000002	32.9	21.325
23	19.85	17.150000000000002	41.425	21.575
24	45.95	10.025	25.1	18.925
25	51.824999999999996	20.05	19.425	8.7
26	34.2335583895974	34.93373343335834	12.003000750187546	18.829707426856714
27	23.549999999999997	41.6	17.775	17.075000000000003
28	14.35	29.525000000000002	42.3	13.825000000000001
29	26.974999999999998	16.85	43.75	12.425
30	13.55	10.525	35.825	40.1
31	13.225000000000001	9.675	43.15	33.95
32	21.91095547773887	17.358679339669834	25.062531265632813	35.66783391695848
33	37.35	18.4	18.725	25.525
34	54.75	9.925	17.599999999999998	17.724999999999998
35	58.71467866966742	7.62690672668167	19.179794948737182	14.47861965491373
36	47.05	13.475000000000001	30.675	8.799999999999999
37	43.75	16.125	24.75	15.375
38	27.250000000000004	14.799999999999999	14.899999999999999	43.05
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	1.0
22	1.5
23	2.0
24	2.0
25	2.0
26	2.0
27	2.0
28	6.5
29	11.0
30	17.0
31	23.0
32	23.0
33	29.0
34	35.0
35	35.0
36	35.0
37	35.0
38	47.5
39	60.0
40	60.0
41	133.5
42	207.0
43	235.0
44	263.0
45	263.0
46	341.0
47	419.0
48	419.0
49	445.5
50	472.0
51	467.0
52	462.0
53	462.0
54	500.0
55	538.0
56	538.0
57	504.5
58	471.0
59	522.0
60	573.0
61	573.0
62	441.0
63	309.0
64	180.5
65	52.0
66	52.0
67	37.0
68	22.0
69	22.0
70	17.5
71	13.0
72	10.0
73	7.0
74	7.0
75	5.0
76	3.0
77	3.0
78	5.0
79	7.0
80	4.5
81	2.0
82	2.0
83	2.5
84	3.0
85	3.0
86	2.0
87	1.0
88	3.0
89	5.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.22499999999999998
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.025
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.05
33	0.0
34	0.0
35	0.025
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01186758276077	32.425
2	7.432854465958775	5.949999999999999
3	3.372891942535915	4.05
4	1.811367895065584	2.9000000000000004
5	0.9369144284821986	1.875
6	0.6246096189881324	1.5
7	0.6870705808869456	1.925
8	0.8119925046845722	2.6
9	0.3123048094940662	1.125
>10	2.4359775140537163	20.225
>50	0.3123048094940662	9.775
>100	0.24984384759525297	15.65
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGC	181	4.5249999999999995	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAAC	178	4.45	RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC	155	3.875	RNA PCR Primer, Index 16 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAA	112	2.8000000000000003	No Hit
AAAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTG	99	2.475	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA	91	2.275	No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGC	84	2.1	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTG	64	1.6	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA	53	1.325	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGC	48	1.2	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACT	46	1.15	RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGT	42	1.05	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGC	40	1.0	No Hit
AAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTGC	34	0.8500000000000001	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGC	33	0.8250000000000001	No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGT	31	0.775	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTG	30	0.75	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGG	29	0.7250000000000001	No Hit
CATCAAAGTGGAGGCCCTCTCTTTGGAATTCTCGGGTG	28	0.7000000000000001	No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAA	26	0.65	RNA PCR Primer, Index 1 (100% over 23bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGC	25	0.625	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGC	24	0.6	No Hit
CCTGCATGATGACGAACAAATACTGACTTGGAATTCTC	23	0.575	No Hit
ACTCAAACTGGGGGCTCTTTTTGGAATTCTCGGGTGCC	23	0.575	No Hit
GGCAGAGGAGGGCTGTTCTTCCCTGGAATTCTCGGGTG	21	0.525	No Hit
AAATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGC	20	0.5	No Hit
CATCAAAGTGGAGGCCCTCTCTTGGAATTCTCGGGTGC	20	0.5	No Hit
GGCAGAGGAGGGCTGTTCTTCCTGGAATTCTCGGGTGC	20	0.5	No Hit
TCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGGG	16	0.4	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGG	15	0.375	No Hit
GGCAGAGGAGGGCTGTTCTTCTGGAATTCTCGGGTGCC	14	0.35000000000000003	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGC	14	0.35000000000000003	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCC	13	0.325	No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTG	13	0.325	No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGA	13	0.325	RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGT	13	0.325	No Hit
TATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTG	13	0.325	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGTGGAAT	13	0.325	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGT	12	0.3	No Hit
ACTCAAACTGGGGGCTCTTTTGATGGAATTCTCGGGTG	12	0.3	No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCC	11	0.27499999999999997	No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
TTTGGCAATGGTAGAACTCACACCGATGGAATTCTCGG	11	0.27499999999999997	No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCC	10	0.25	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGT	10	0.25	No Hit
ACTCAAACTGGGGGCTCTTTTGTGGAATTCTCGGGTGC	10	0.25	No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTG	10	0.25	No Hit
CTCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGG	9	0.22499999999999998	No Hit
TATGTAGTATGGTCCACATCTTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
AAGGGATTCTGATGTTGGTCACTGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
AATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
TTTGTGACCTGGTCCACTAACCCTGGAATTCTCGGGTG	8	0.2	No Hit
ATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTTGGAAT	8	0.2	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTGGAATTCTCGGGTG	8	0.2	No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGC	8	0.2	No Hit
CTGCATGATGACGAACAAATACTGACTTGGAATTCTCG	8	0.2	No Hit
TTGTGGCAACAATGGCACGGCAAGGGCGCCTTTTTGGA	8	0.2	No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGC	8	0.2	No Hit
CTCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGG	8	0.2	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCC	8	0.2	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTTGGAATTCT	8	0.2	No Hit
AAATCTACCTGCCTCTGCCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TATGGCACTGGTAGAATTCACTTGGAATTCTCGGGTGC	8	0.2	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGT	8	0.2	No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
CAGTGCAATAGTATTGTCAAAGCTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
ACTCAAACTGTGTGACATTTTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
AGTGCCGCAGAGTTTGTAGTGTTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTTGGA	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTG	7	0.17500000000000002	No Hit
TTTGGCAATGGTAGAACTCACACCGGTGGAATTCTCGG	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGT	7	0.17500000000000002	No Hit
TTTGGCAATGGTAGAACTCACACCGTTGGAATTCTCGG	7	0.17500000000000002	No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTG	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGG	6	0.15	No Hit
ATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTGC	6	0.15	No Hit
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGG	6	0.15	No Hit
TGAGGTTGGTGTACTGTGTGTGATGGAATTCTCGGGTG	6	0.15	No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGT	6	0.15	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCC	6	0.15	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGC	5	0.125	No Hit
AGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGTGGAAT	5	0.125	No Hit
ACTCAAACTGGGGGCTCTTTTGTTGGAATTCTCGGGTG	5	0.125	No Hit
TGTCTTGCAGGCCGTCATGCATGGAATTCTCGGGTGCC	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC	5	0.125	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGT	5	0.125	No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTG	5	0.125	No Hit
AAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGTG	5	0.125	No Hit
GCATGATGACGAACAAATACTGACTTGGAATTCTCGGG	5	0.125	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGTGG	5	0.125	No Hit
AGATCTACCTGCCTCTGCCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGTG	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGT	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGC	5	0.125	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	4.225	0.0	0.0	0.0
2	0.0	4.25	0.0	0.0	0.0
3	0.0	4.25	0.0	0.0	0.0
4	0.0	4.3	0.0	0.0	0.0
5	0.0	4.4	0.0	0.0	0.0
6	0.0	4.725	0.0	0.0	0.0
7	0.0	4.925	0.0	0.0	0.0
8	0.0	5.175	0.0	0.0	0.0
9	0.0	5.3	0.0	0.0	0.0
10	0.0	5.65	0.0	0.0	0.0
11	0.0	5.9	0.0	0.0	0.0
12	0.0	6.525	0.0	0.0	0.0
13	0.0	6.85	0.0	0.0	0.0
14	0.0	8.125	0.0	0.0	0.0
15	0.0	13.3	0.0	0.0	0.0
16	0.0	14.15	0.0	0.0	0.0
17	0.0	14.825	0.0	0.0	0.0
18	0.0	15.675	0.0	0.0	0.0
19	0.0	16.925	0.0	0.0	0.0
20	0.0	17.625	0.0	0.0	0.0
21	0.0	19.125	0.0	0.0	0.0
22	0.0	25.0	0.0	0.0	0.0
23	0.0	46.275	0.0	0.0	0.0
24	0.0	59.45	0.0	0.0	0.0
25	0.0	66.1	0.0	0.0	0.0
26	0.0	69.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	25	2.4147211E-4	32.0	1
CGCTGAT	20	0.0033271667	32.0	28
TGCACTT	30	1.7408885E-5	32.0	4
TTGTCCC	30	1.7408885E-5	32.0	9
TTGCACT	40	8.843199E-8	32.0	3
ACTTGTC	30	1.7408885E-5	32.0	7
CCTGTTG	20	0.0033271667	32.0	18
GCGACCT	25	2.4147211E-4	32.0	2
GCGACCC	25	2.4147211E-4	32.0	22
TGTCCCG	30	1.7408885E-5	32.0	10
CCCGGCC	40	8.843199E-8	32.0	13
CCGCTGA	25	2.4147211E-4	32.0	27
GGCGACC	25	2.4147211E-4	32.0	21
ACCCGCT	25	2.4147211E-4	32.0	25
TATTGCA	40	8.843199E-8	32.0	1
CCCGCTG	25	2.4147211E-4	32.0	26
CGTGGCG	25	2.4147211E-4	32.0	18
ATCAGAC	25	2.4147211E-4	32.0	12
GTCCCGG	40	8.843199E-8	32.0	11
GGCCTGT	30	1.7408885E-5	32.0	16
>>END_MODULE
SRR13079630 read2 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079630_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01175	32.0	32.0	32.0	32.0	32.0
2	30.90075	32.0	32.0	32.0	32.0	32.0
3	30.913	32.0	32.0	32.0	32.0	32.0
4	31.03175	32.0	32.0	32.0	32.0	32.0
5	30.998	32.0	32.0	32.0	32.0	32.0
6	34.4395	36.0	36.0	36.0	32.0	36.0
7	34.34675	36.0	36.0	36.0	32.0	36.0
8	34.3005	36.0	36.0	36.0	32.0	36.0
9	34.2585	36.0	36.0	36.0	32.0	36.0
10	34.26625	36.0	36.0	36.0	32.0	36.0
11	34.22825	36.0	36.0	36.0	32.0	36.0
12	34.26975	36.0	36.0	36.0	32.0	36.0
13	34.215	36.0	36.0	36.0	32.0	36.0
14	34.31075	36.0	36.0	36.0	32.0	36.0
15	34.1455	36.0	36.0	36.0	32.0	36.0
16	34.3705	36.0	36.0	36.0	32.0	36.0
17	34.211	36.0	36.0	36.0	32.0	36.0
18	33.9	36.0	36.0	36.0	32.0	36.0
19	34.2225	36.0	36.0	36.0	32.0	36.0
20	34.053	36.0	36.0	36.0	32.0	36.0
21	34.175	36.0	36.0	36.0	32.0	36.0
22	34.023	36.0	36.0	36.0	32.0	36.0
23	33.93225	36.0	36.0	36.0	32.0	36.0
24	33.73625	36.0	36.0	36.0	32.0	36.0
25	34.00075	36.0	36.0	36.0	32.0	36.0
26	34.21625	36.0	36.0	36.0	32.0	36.0
27	34.14225	36.0	36.0	36.0	32.0	36.0
28	33.9775	36.0	36.0	36.0	32.0	36.0
29	33.94575	36.0	36.0	36.0	32.0	36.0
30	33.8905	36.0	36.0	36.0	32.0	36.0
31	33.81225	36.0	36.0	36.0	32.0	36.0
32	33.828	36.0	36.0	36.0	32.0	36.0
33	33.86	36.0	36.0	36.0	32.0	36.0
34	34.198	36.0	36.0	36.0	32.0	36.0
35	33.78225	36.0	36.0	36.0	32.0	36.0
36	33.864	36.0	36.0	36.0	32.0	36.0
37	33.93575	36.0	36.0	36.0	32.0	36.0
38	32.8905	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	8.0
7	0.0
8	0.0
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	1.0
19	3.0
20	4.0
21	8.0
22	9.0
23	14.0
24	22.0
25	29.0
26	39.0
27	68.0
28	83.0
29	116.0
30	142.0
31	202.0
32	234.0
33	364.0
34	798.0
35	1852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.32032032032032	36.36136136136136	21.496496496496498	21.82182182182182
2	30.115057528764382	22.086043021510758	12.656328164082039	35.14257128564282
3	20.674999999999997	41.699999999999996	19.05	18.575
4	29.7	18.675	12.675	38.95
5	42.825	21.375	18.75	17.05
6	36.0	17.025000000000002	17.7	29.275000000000002
7	34.76953907815631	21.7685370741483	12.074148296593187	31.387775551102205
8	35.245490981963925	29.909819639278556	15.531062124248496	19.31362725450902
9	31.2280701754386	30.350877192982455	19.649122807017545	18.771929824561404
10	26.691729323308273	20.37593984962406	19.172932330827066	33.7593984962406
11	31.361243419403362	23.31411381298571	17.623464527450487	27.70117824016044
12	20.596341768980206	17.514407416687547	29.240791781508396	32.64845903282385
13	26.11528822055138	30.50125313283208	13.258145363408522	30.125313283208023
14	28.66098294884654	26.68004012036108	19.207622868605817	25.45135406218656
15	29.832038104788168	25.64552519428428	20.907495612935573	23.614941087991976
16	29.714142427281846	26.37913741223671	25.55165496489468	18.35506519558676
17	32.31309583542398	19.593577521324637	31.008529854490718	17.084796788760663
18	18.726497869140136	17.974429681624468	25.04387064427175	38.255201804963654
19	25.068939583855602	27.801453998495862	21.684632740035095	25.444973677613437
20	20.647265429001504	23.68289011540391	32.137481184144505	23.532363271450073
21	17.06766917293233	22.355889724310778	35.13784461152882	25.438596491228072
22	22.63725244422161	31.76234645274505	29.405866131862624	16.194534971170718
23	42.1765295887663	20.13540621865597	19.53360080240722	18.154463390170513
24	22.907268170426065	45.18796992481203	16.99248120300752	14.912280701754385
25	18.576084231637	28.252694911005268	33.31662070694409	19.85460015041364
26	21.13841524573721	18.05416248746239	25.501504513540624	35.30591775325978
27	46.7134972403412	13.070747616658304	19.568489713998996	20.647265429001504
28	31.08209892041175	9.13884007029877	39.61837810695455	20.160682902334923
29	30.3714859437751	13.227911646586346	24.92469879518072	31.475903614457827
30	44.74014561888024	10.143108209892041	21.139844338438362	23.976901832789355
31	47.37634948531258	22.395179512929953	13.030379111222695	17.198091890534773
32	35.15683814303638	32.848180677540775	10.41405269761606	21.580928481806776
33	22.495606326889277	24.604569420035148	15.842329902083858	37.05749435099172
34	24.981169972382627	13.93422043685664	38.36304293246297	22.721566658297764
35	35.7429718875502	14.60843373493976	27.56024096385542	22.08835341365462
36	25.552208835341368	18.097389558232933	45.55722891566265	10.793172690763052
37	26.83906603063018	31.107205623901578	26.638212402711524	15.415515942756716
38	40.572432839568165	32.23700728094401	15.867436605573687	11.323123273914135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	0.5
5	0.0
6	0.0
7	1.5
8	3.0
9	3.0
10	3.0
11	3.0
12	2.0
13	1.0
14	1.0
15	1.0
16	1.0
17	1.5
18	2.0
19	2.0
20	1.5
21	1.0
22	0.5
23	0.0
24	0.0
25	1.0
26	2.0
27	2.0
28	3.0
29	4.0
30	4.5
31	5.0
32	5.0
33	11.5
34	18.0
35	18.0
36	20.5
37	23.0
38	34.0
39	45.0
40	45.0
41	128.0
42	211.0
43	200.0
44	189.0
45	189.0
46	276.5
47	364.0
48	364.0
49	522.0
50	680.0
51	603.0
52	526.0
53	526.0
54	622.5
55	719.0
56	719.0
57	588.5
58	458.0
59	378.5
60	299.0
61	299.0
62	236.5
63	174.0
64	175.0
65	176.0
66	176.0
67	112.5
68	49.0
69	49.0
70	28.0
71	7.0
72	10.5
73	14.0
74	14.0
75	9.5
76	5.0
77	5.0
78	5.5
79	6.0
80	5.5
81	5.0
82	5.0
83	4.0
84	3.0
85	3.0
86	2.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.2
8	0.2
9	0.25
10	0.25
11	0.27499999999999997
12	0.22499999999999998
13	0.25
14	0.3
15	0.27499999999999997
16	0.3
17	0.35000000000000003
18	0.27499999999999997
19	0.27499999999999997
20	0.35000000000000003
21	0.25
22	0.27499999999999997
23	0.3
24	0.25
25	0.27499999999999997
26	0.3
27	0.35000000000000003
28	0.42500000000000004
29	0.4
30	0.42500000000000004
31	0.42500000000000004
32	0.375
33	0.42500000000000004
34	0.42500000000000004
35	0.4
36	0.4
37	0.42500000000000004
38	0.42500000000000004
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.05
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09500609013398	33.7
2	6.82095006090134	5.6000000000000005
3	3.3495736906211935	4.125
4	1.5834348355663823	2.6
5	0.7917174177831912	1.625
6	0.6090133982947624	1.5
7	0.9744214372716199	2.8000000000000003
8	0.48721071863580995	1.6
9	0.3654080389768575	1.35
>10	2.3751522533495737	19.85
>50	0.3045066991473812	9.65
>100	0.24360535931790497	15.6
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	184	4.6	No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGA	175	4.375	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	153	3.8249999999999997	Illumina RNA PCR Primer (100% over 33bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	112	2.8000000000000003	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTA	100	2.5	No Hit
TCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	87	2.175	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	87	2.175	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTA	61	1.525	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATC	51	1.275	No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAG	48	1.2	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGT	43	1.075	No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAA	42	1.05	Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAG	38	0.95	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAG	34	0.8500000000000001	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAG	34	0.8500000000000001	No Hit
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	32	0.8	No Hit
GTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTA	30	0.75	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTG	28	0.7000000000000001	No Hit
AGGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAG	27	0.675	No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTG	26	0.65	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGA	23	0.575	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTA	23	0.575	No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAG	23	0.575	No Hit
GGAGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAG	21	0.525	No Hit
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGA	21	0.525	No Hit
GGGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTA	20	0.5	No Hit
GGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAG	19	0.475	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAG	19	0.475	No Hit
TCCGGAGCACCACATCGATCTAAGAGATCGTCGGACTG	16	0.4	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTG	15	0.375	No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAG	14	0.35000000000000003	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGT	14	0.35000000000000003	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTA	13	0.325	No Hit
AAAGTCTCGCTCTCTGCCCCTCAGATCGTCGGACTGTA	13	0.325	No Hit
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCT	13	0.325	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAGA	12	0.3	No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGT	12	0.3	No Hit
CCACGGGAACGGATCGTCGGACTGTAGAACTCTGAACG	12	0.3	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGA	12	0.3	No Hit
CAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGA	12	0.3	No Hit
ACTGAGGGGCCTCAGACCGAGCTGATCGTCGGACTGTA	11	0.27499999999999997	No Hit
CGTACGCTATACGGTCTACTAGATCGTCGGACTGTAGA	11	0.27499999999999997	No Hit
TCAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTA	11	0.27499999999999997	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGT	11	0.27499999999999997	No Hit
TCGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACT	11	0.27499999999999997	No Hit
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	10	0.25	No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGA	10	0.25	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAG	10	0.25	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGAC	9	0.22499999999999998	No Hit
GTGACCAACATCAGAATCCCTTGATCGTCGGACTGTAG	9	0.22499999999999998	No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGT	9	0.22499999999999998	No Hit
AGATGTGGACCATACTACATAGATCGTCGGACTGTAGA	9	0.22499999999999998	No Hit
CCGGAGCACCACATCGATCTAAGAGGATCGTCGGACTG	9	0.22499999999999998	No Hit
GGAGGCAGAGGCAGGTAGATTGATCGTCGGACTGTAGA	9	0.22499999999999998	No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAG	8	0.2	No Hit
ACTCAGAATCACCAGAAATCATCATTACTGATCGTCGG	8	0.2	No Hit
AGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAGAA	8	0.2	No Hit
AAAAGGCGCCCTTGCCGTGCCATTGTTGCCACAAGATC	8	0.2	No Hit
GGGTTAGTGGACCAGGTCACAAAGATCGTCGGACTGTA	8	0.2	No Hit
AGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAG	8	0.2	No Hit
AGGAGCGAGCGACCAAAGGAACCATAACTGATGATCGT	8	0.2	No Hit
TCCGGAGCACCACATCGATCTAAGAGGATCGTCGGACT	8	0.2	No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGT	7	0.17500000000000002	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
GCTTTGACAATACTATTGCACTGGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
GACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGA	7	0.17500000000000002	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGT	7	0.17500000000000002	No Hit
CCGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACT	7	0.17500000000000002	No Hit
CCACGGGAACGTGATCGTCGGACTGTAGAACTCTGAAC	7	0.17500000000000002	Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
AGGGGTTCACCGAGCAACATTCGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGGATC	7	0.17500000000000002	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
ACGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACT	7	0.17500000000000002	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
TGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
GAGTGTGACCAACATCAGAATCCCTTGATCGTCGGACT	6	0.15	No Hit
AAAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATC	6	0.15	No Hit
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTC	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTA	6	0.15	No Hit
TCACACACAGTACACCAACCTCAGATCGTCGGACTGTA	6	0.15	No Hit
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	6	0.15	No Hit
CAGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAG	6	0.15	No Hit
AACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	6	0.15	No Hit
TTTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTG	6	0.15	No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGATCGTCGGAC	6	0.15	No Hit
CGTACGCTATACGGTCTACTATGATCGTCGGACTGTAG	5	0.125	No Hit
CTACCTGCACGAACAGCACTTTGGATCGTCGGACTGTA	5	0.125	No Hit
CTTCAGGTAGTCAGTATTTGTTCGTCATCATGCAGGGA	5	0.125	No Hit
CTTCAGGTAGTCAGTATTTGTTCGTCATCATGCAGGAT	5	0.125	No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAG	5	0.125	No Hit
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCG	5	0.125	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTA	5	0.125	No Hit
CCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTGATCGT	5	0.125	No Hit
TGCATGACGGCCTGCAAGACAGATCGTCGGACTGTAGA	5	0.125	No Hit
ACAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTA	5	0.125	No Hit
TTTCCGGAGCACCACATCGATCTAAGAGATCGTCGGAC	5	0.125	No Hit
AGTCAGTATTTGTTCGTCATCATGCGATCGTCGGACTG	5	0.125	No Hit
AGTCAGATCCAATCTGTCATCACTGCTGATCGTCGGAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.125	0.0	0.0
2	0.0	0.0	4.15	0.0	0.0
3	0.0	0.0	4.15	0.0	0.0
4	0.0	0.0	4.2	0.0	0.0
5	0.0	0.0	4.275	0.0	0.0
6	0.0	0.0	4.6	0.0	0.0
7	0.0	0.0	4.8	0.0	0.0
8	0.0	0.0	5.025	0.0	0.0
9	0.0	0.0	5.175	0.0	0.0
10	0.0	0.0	5.525	0.0	0.0
11	0.0	0.0	5.75	0.0	0.0
12	0.0	0.0	6.375	0.0	0.0
13	0.0	0.0	6.7	0.0	0.0
14	0.0	0.0	7.875	0.0	0.0
15	0.0	0.0	12.8	0.0	0.0
16	0.0	0.0	13.7	0.0	0.0
17	0.0	0.0	14.375	0.0	0.0
18	0.0	0.0	15.175	0.0	0.0
19	0.0	0.0	16.4	0.0	0.0
20	0.0	0.0	17.125	0.0	0.0
21	0.0	0.0	18.675	0.0	0.0
22	0.0	0.0	24.475	0.0	0.0
23	0.0	0.0	45.65	0.0	0.0
24	0.0	0.0	58.55	0.0	0.0
25	0.0	0.0	64.9	0.0	0.0
26	0.0	0.0	68.225	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGG	30	1.7408885E-5	32.0	8
CGGGAAC	35	1.2459041E-6	32.0	4
ACGGGAA	40	8.843199E-8	32.0	3
GGGAACG	35	1.2459041E-6	32.0	5
GGAACGT	30	1.7408885E-5	32.0	6
CACGGGA	40	8.843199E-8	32.0	2
CCACGGG	45	2.4896326E-7	28.444445	1
GAGGTCG	25	0.00990922	25.6	25
GGGTCGC	25	0.00990922	25.6	6
CTGAGGT	25	0.00990922	25.6	23
ACGTCTG	25	0.00990922	25.6	14
CCACGTC	25	0.00990922	25.6	12
GTCGCGG	25	0.00990922	25.6	28
GTCGCCA	25	0.00990922	25.6	8
CACGTCT	25	0.00990922	25.6	13
TGATCTG	25	0.00990922	25.6	19
TCGCGGA	25	0.00990922	25.6	29
CGCCACG	25	0.00990922	25.6	10
GTCTGAT	25	0.00990922	25.6	16
CTGATCT	25	0.00990922	25.6	18
>>END_MODULE
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SRR ids: ['SRR13079630.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_3cyg2u6v
SRR13079630.sra spots: 9303781
blocks: [[1, 145371], [145372, 290742], [290743, 436113], [436114, 581484], [581485, 726855], [726856, 872226], [872227, 1017597], [1017598, 1162968], [1162969, 1308339], [1308340, 1453710], [1453711, 1599081], [1599082, 1744452], [1744453, 1889823], [1889824, 2035194], [2035195, 2180565], [2180566, 2325936], [2325937, 2471307], [2471308, 2616678], [2616679, 2762049], [2762050, 2907420], [2907421, 3052791], [3052792, 3198162], [3198163, 3343533], [3343534, 3488904], [3488905, 3634275], [3634276, 3779646], [3779647, 3925017], [3925018, 4070388], [4070389, 4215759], [4215760, 4361130], [4361131, 4506501], [4506502, 4651872], [4651873, 4797243], [4797244, 4942614], [4942615, 5087985], [5087986, 5233356], [5233357, 5378727], [5378728, 5524098], [5524099, 5669469], [5669470, 5814840], [5814841, 5960211], [5960212, 6105582], [6105583, 6250953], [6250954, 6396324], [6396325, 6541695], [6541696, 6687066], [6687067, 6832437], [6832438, 6977808], [6977809, 7123179], [7123180, 7268550], [7268551, 7413921], [7413922, 7559292], [7559293, 7704663], [7704664, 7850034], [7850035, 7995405], [7995406, 8140776], [8140777, 8286147], [8286148, 8431518], [8431519, 8576889], [8576890, 8722260], [8722261, 8867631], [8867632, 9013002], [9013003, 9158373], [9158374, 9303781]]
SRR13079630 file size 1079032
SRR13079630 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13079630 SRR13079630_1.fastq SRR13079630_2.fastq
Input file:	SRR13079630_1.fastq
Paired file:	SRR13079630_2.fastq
trimmed:	SRR13079630-trimmed-pair1.fastq, SRR13079630-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:34:00 2021 >> started

Tue Apr 20 16:34:18 2021 >> done (18.518s)
9303781 read pairs processed; of these:
   4795 ( 0.05%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
9298986 (99.95%) read pairs available; of these:
   5549 ( 0.06%) trimmed read pairs available after processing
9293437 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 26	      1	  0.00%
 27	     10	  0.00%
 28	    141	  0.00%
 29	    654	  0.01%
 30	    199	  0.00%
 31	    513	  0.01%
 32	    530	  0.01%
 33	    160	  0.00%
 34	    363	  0.00%
 35	    123	  0.00%
 36	    940	  0.01%
 37	   1915	  0.02%
 38	9293437	 99.94%
9298986 reads passed initial QC


criterion=sequence-density
sequence-density=70.85
sequence-density-rank=1
fanout-score=20.50
fanout-score-rank=1
prefix-density=72.21
prefix-fanout=20.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC


criterion=fanout-score
sequence-density=70.85
sequence-density-rank=1
fanout-score=20.50
fanout-score-rank=1
prefix-density=72.21
prefix-fanout=20.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC


criterion=sequence-density
sequence-density=69.00
sequence-density-rank=1
fanout-score=18.27
fanout-score-rank=1
prefix-density=70.43
prefix-fanout=17.9
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG


criterion=fanout-score
sequence-density=69.00
sequence-density-rank=1
fanout-score=18.27
fanout-score-rank=1
prefix-density=70.43
prefix-fanout=17.9
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC -y GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG -o SRR13079630 SRR13079630_1.fastq SRR13079630_2.fastq
Input file:	SRR13079630_1.fastq
Paired file:	SRR13079630_2.fastq
trimmed:	SRR13079630-trimmed-pair1.fastq, SRR13079630-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC
-- paired 3' end adapter sequence (-y):	GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:35:08 2021 >> started

Tue Apr 20 16:35:25 2021 >> done (16.585s)
9033301 read pairs processed; of these:
1115889 (12.35%) short read pairs filtered out after trimming by size control
 464743 ( 5.14%) empty read pairs filtered out after trimming by size control
7452669 (82.50%) read pairs available; of these:
7026991 (94.29%) trimmed read pairs available after processing
 425678 ( 5.71%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 100349	  1.35%
 19	  70565	  0.95%
 20	 183874	  2.47%
 21	 563688	  7.56%
 22	1953725	 26.22%
 23	1098048	 14.73%
 24	 571307	  7.67%
 25	 303671	  4.07%
 26	 178440	  2.39%
 27	 162696	  2.18%
 28	 189903	  2.55%
 29	 122380	  1.64%
 30	 110874	  1.49%
 31	 101424	  1.36%
 32	 167225	  2.24%
 33	 422181	  5.66%
 34	 438118	  5.88%
 35	 109993	  1.48%
 36	  98031	  1.32%
 37	  82054	  1.10%
 38	 424123	  5.69%


criterion=sequence-density
sequence-density=11.52
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=TATTGCACTTGTCCCGGCCTGTAAA


criterion=fanout-score
sequence-density=0.49
sequence-density-rank=18
fanout-score=31.65
fanout-score-rank=1
prefix-density=12.82
prefix-fanout=1.2
sequence=GTCCCGGCCTGA


criterion=sequence-density
sequence-density=3.19
sequence-density-rank=1
fanout-score=4.33
fanout-score-rank=2
prefix-density=0.10
prefix-fanout=4.3
sequence=AGCCAAGCTCAGACGGATCCGA


criterion=fanout-score
sequence-density=0.39
sequence-density-rank=12
fanout-score=13.26
fanout-score-rank=1
prefix-density=0.39
prefix-fanout=13.3
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGA
SRR13079630 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:36:37
                             Started mapping on |	Apr 20 16:36:38
                                    Finished on |	Apr 20 16:37:10
       Mapping speed, Million of reads per hour |	868.31

                          Number of input reads |	7718354
                      Average input read length |	52
                                    UNIQUE READS:
                   Uniquely mapped reads number |	5158124
                        Uniquely mapped reads % |	66.83%
                          Average mapped length |	47.17
                       Number of splices: Total |	13228
            Number of splices: Annotated (sjdb) |	6737
                       Number of splices: GT/AG |	10926
                       Number of splices: GC/AG |	518
                       Number of splices: AT/AC |	14
               Number of splices: Non-canonical |	1770
                      Mismatch rate per base, % |	0.19%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.20
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	618299
             % of reads mapped to multiple loci |	8.01%
        Number of reads mapped to too many loci |	70124
             % of reads mapped to too many loci |	0.91%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	20.08%
                     % of reads unmapped: other |	4.17%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1941932	1941932	1941932
N_multimapping	618299	618299	618299
N_noFeature	590272	667258	3653421
N_ambiguous	2575792	1310673	213156
UnstrandedReadsAssigned:1992060 PositiveStrandReadsAssigned:3180193 NegativeStrandReadsAssigned:1291547
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13079630 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079630-trimmed-pair1.fastq
                             SRR13079630-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 7,718,354 reads, 2,084,490 reads pseudoaligned
[quant] estimated average fragment length: 27.1608
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,277 rounds

 109283 SRR13079630.ke.tsv
  52636 SRR13079630.se.tsv
 161919 total
==> SRR13079630.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079630.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079630 completed mapping pipeline successfully
