Starting /dee2/code/volunteer_pipeline.sh SRR13079631
    current disk space = 414472126464
    free memory = 73642632 
SRR13079631 SRAfilesize
e58d93883cee01cbfcaa26e35ec1f44f  SRR13079631.sra
SRR13079631.sra file validated
SRR13079631 is paired end
SRR13079631 is conventional basespace
SRR13079631 read1 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63775	32.0	32.0	32.0	32.0	32.0
2	31.42675	32.0	32.0	32.0	32.0	32.0
3	31.41925	32.0	32.0	32.0	32.0	32.0
4	31.42425	32.0	32.0	32.0	32.0	32.0
5	31.3375	32.0	32.0	32.0	32.0	32.0
6	34.67775	36.0	36.0	36.0	32.0	36.0
7	34.284	36.0	36.0	36.0	32.0	36.0
8	34.57	36.0	36.0	36.0	32.0	36.0
9	34.6295	36.0	36.0	36.0	32.0	36.0
10	34.65675	36.0	36.0	36.0	32.0	36.0
11	34.4605	36.0	36.0	36.0	32.0	36.0
12	34.735	36.0	36.0	36.0	32.0	36.0
13	34.87025	36.0	36.0	36.0	32.0	36.0
14	34.82225	36.0	36.0	36.0	32.0	36.0
15	34.71475	36.0	36.0	36.0	32.0	36.0
16	34.699	36.0	36.0	36.0	32.0	36.0
17	34.72925	36.0	36.0	36.0	32.0	36.0
18	34.80025	36.0	36.0	36.0	32.0	36.0
19	34.82375	36.0	36.0	36.0	32.0	36.0
20	34.76075	36.0	36.0	36.0	32.0	36.0
21	34.8275	36.0	36.0	36.0	32.0	36.0
22	34.7435	36.0	36.0	36.0	32.0	36.0
23	34.6605	36.0	36.0	36.0	32.0	36.0
24	34.73075	36.0	36.0	36.0	32.0	36.0
25	34.4655	36.0	36.0	36.0	32.0	36.0
26	34.56775	36.0	36.0	36.0	32.0	36.0
27	34.5345	36.0	36.0	36.0	32.0	36.0
28	34.36275	36.0	36.0	36.0	32.0	36.0
29	34.52925	36.0	36.0	36.0	32.0	36.0
30	34.5425	36.0	36.0	36.0	32.0	36.0
31	34.5055	36.0	36.0	36.0	32.0	36.0
32	34.44125	36.0	36.0	36.0	32.0	36.0
33	34.25725	36.0	36.0	36.0	32.0	36.0
34	34.42675	36.0	36.0	36.0	32.0	36.0
35	34.426	36.0	36.0	36.0	32.0	36.0
36	34.547	36.0	36.0	36.0	32.0	36.0
37	34.597	36.0	36.0	36.0	32.0	36.0
38	34.46425	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	4.0
24	7.0
25	20.0
26	23.0
27	34.0
28	57.0
29	89.0
30	112.0
31	181.0
32	247.0
33	314.0
34	758.0
35	2150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.75	26.924999999999997	38.425	19.900000000000002
2	20.150000000000002	46.300000000000004	16.325	17.224999999999998
3	20.849999999999998	20.349999999999998	39.225	19.575
4	29.025000000000002	17.299999999999997	34.025	19.650000000000002
5	42.15	24.975	18.325	14.549999999999999
6	25.65772989225758	22.074668003006764	15.309446254071663	36.95815585066399
7	20.9	39.6	18.0	21.5
8	19.625	21.25	21.275	37.85
9	12.8	23.025000000000002	40.65	23.525
10	30.275000000000002	12.475	36.4	20.849999999999998
11	51.175000000000004	17.424999999999997	15.25	16.150000000000002
12	20.9	26.950000000000003	35.025	17.125
13	25.324999999999996	17.7	22.925	34.050000000000004
14	25.0	17.875	24.8	32.324999999999996
15	19.225	23.5	22.575	34.699999999999996
16	37.65	19.575	28.249999999999996	14.524999999999999
17	36.475	22.475	22.25	18.8
18	20.549999999999997	17.0	21.85	40.6
19	23.375	18.825	20.325	37.475
20	25.074999999999996	14.575	44.800000000000004	15.55
21	36.625	12.85	23.549999999999997	26.974999999999998
22	21.625	19.05	39.2	20.125
23	24.0	16.925	42.525	16.55
24	50.125	12.55	26.924999999999997	10.4
25	51.125	20.849999999999998	17.075000000000003	10.95
26	30.515257628814407	39.769884942471236	15.532766383191596	14.18209104552276
27	21.725	45.5	19.925	12.85
28	13.950000000000001	28.025	44.7	13.325000000000001
29	16.925	19.625	49.75	13.700000000000001
30	16.375	11.075	35.125	37.425000000000004
31	16.425	9.525	47.225	26.825
32	23.1	9.65	27.400000000000002	39.85
33	42.25	13.475000000000001	22.525000000000002	21.75
34	53.7	9.875	21.675	14.75
35	59.175	7.675	23.325000000000003	9.825000000000001
36	45.675	8.725	35.275	10.325
37	49.125	11.075	23.724999999999998	16.075
38	28.975	15.825	16.325	38.875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	2.5
21	5.0
22	3.5
23	2.0
24	2.0
25	3.0
26	4.0
27	4.0
28	7.5
29	11.0
30	14.5
31	18.0
32	18.0
33	29.5
34	41.0
35	41.0
36	49.0
37	57.0
38	76.0
39	95.0
40	95.0
41	196.5
42	298.0
43	306.0
44	314.0
45	314.0
46	343.0
47	372.0
48	372.0
49	469.5
50	567.0
51	601.0
52	635.0
53	635.0
54	663.5
55	692.0
56	692.0
57	463.5
58	235.0
59	288.5
60	342.0
61	342.0
62	278.5
63	215.0
64	128.5
65	42.0
66	42.0
67	30.0
68	18.0
69	18.0
70	15.5
71	13.0
72	10.5
73	8.0
74	8.0
75	7.0
76	6.0
77	6.0
78	3.5
79	1.0
80	1.0
81	1.0
82	1.0
83	1.5
84	2.0
85	2.0
86	1.5
87	1.0
88	2.5
89	4.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.22499999999999998
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.05
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00557103064067	36.8
2	6.796657381615599	6.1
3	3.454038997214485	4.65
4	1.7270194986072425	3.1
5	1.16991643454039	2.625
6	0.724233983286908	1.95
7	0.22284122562674097	0.7000000000000001
8	0.5571030640668524	2.0
9	0.5571030640668524	2.25
>10	2.395543175487465	20.674999999999997
>50	0.22284122562674097	7.675
>100	0.1671309192200557	11.475
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGC	229	5.7250000000000005	No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGC	118	2.9499999999999997	No Hit
AAAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTG	112	2.8000000000000003	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA	87	2.175	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAA	86	2.15	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTG	81	2.025	No Hit
AAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTGC	53	1.325	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGC	47	1.175	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA	42	1.05	No Hit
CCTGCATGATGACGAACAAATACTGACTTGGAATTCTC	40	1.0	No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGT	37	0.9249999999999999	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGC	36	0.8999999999999999	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGC	33	0.8250000000000001	No Hit
CATCAAAGTGGAGGCCCTCTCTTGGAATTCTCGGGTGC	33	0.8250000000000001	No Hit
ACTCAAACTGGGGGCTCTTTTTGGAATTCTCGGGTGCC	30	0.75	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGC	28	0.7000000000000001	No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTG	24	0.6	No Hit
AAATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGC	23	0.575	No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGT	23	0.575	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGG	20	0.5	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGT	20	0.5	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTGGAATTCTCGGGTG	19	0.475	No Hit
GGCAGAGGAGGGCTGTTCTTCCCTGGAATTCTCGGGTG	19	0.475	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCC	18	0.44999999999999996	No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTG	18	0.44999999999999996	No Hit
TCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGGG	17	0.42500000000000004	No Hit
AAATCTACCTGCCTCTGCCTTGGAATTCTCGGGTGCCA	17	0.42500000000000004	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGTGGAAT	17	0.42500000000000004	No Hit
ACTCAAACTGGGGGCTCTTTTGATGGAATTCTCGGGTG	16	0.4	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGC	15	0.375	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTTGGAATTCT	14	0.35000000000000003	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGT	14	0.35000000000000003	No Hit
CATCAAAGTGGAGGCCCTCTCTTTGGAATTCTCGGGTG	13	0.325	No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGC	13	0.325	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCC	13	0.325	No Hit
AGCAGTGATGACAGATTGGATCTGACTTGGAATTCTCG	13	0.325	No Hit
GGCAGAGGAGGGCTGTTCTTCCTGGAATTCTCGGGTGC	13	0.325	No Hit
TTTGTGACCTGGTCCACTAACCCTGGAATTCTCGGGTG	12	0.3	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGG	12	0.3	No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAAC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 24bp)
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTGGAA	11	0.27499999999999997	No Hit
CTGCATGATGACGAACAAATACTGACTTGGAATTCTCG	11	0.27499999999999997	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGT	11	0.27499999999999997	No Hit
GGCAGAGGAGGGCTGTTCTTCTGGAATTCTCGGGTGCC	11	0.27499999999999997	No Hit
AGTGCCGCAGAGTTTGTAGTGTTGGAATTCTCGGGTGC	11	0.27499999999999997	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
AAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGTG	11	0.27499999999999997	No Hit
ATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTTGGAAT	10	0.25	No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCC	10	0.25	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCC	10	0.25	No Hit
CTCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGG	9	0.22499999999999998	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTGGAATTCTCG	9	0.22499999999999998	No Hit
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCC	9	0.22499999999999998	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGT	9	0.22499999999999998	No Hit
ACTCAAACTGGGGGCTCTTTTGTGGAATTCTCGGGTGC	9	0.22499999999999998	No Hit
TCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGGT	9	0.22499999999999998	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGT	9	0.22499999999999998	No Hit
TGTCTTGCAGGCCGTCATGCTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
AAGGGATTCTGATGTTGGTCACATGGAATTCTCGGGTG	8	0.2	No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTG	8	0.2	No Hit
AAAGTGCTTCCCTTTTGTGTGTTGGAATTCTCGGGTGC	8	0.2	No Hit
AAGGGATTCTGATGTTGGTCACACTGGAATTCTCGGGT	8	0.2	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TCTTAGATCGATGTGGTGCTCCGGAGTGGAATTCTCGG	8	0.2	No Hit
TGAGGGGCAGAGAGCGAGACTTTTTGGAATTCTCGGGT	8	0.2	No Hit
CTCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGG	8	0.2	No Hit
ACAGACAGTTAGGCCTCACCTAGGCTTTTTGGAATTCT	8	0.2	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGTGG	8	0.2	No Hit
TGTCTTGCAGGCCGTCATGCATGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GCATGATGACGAACAAATACTGACTTGGAATTCTCGGG	7	0.17500000000000002	No Hit
TATTGCACTTGTCCCGGCCTGAATGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
TCGTTAGTTCATTTCTGACAGGTGGCACCATGGAATTC	7	0.17500000000000002	No Hit
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTG	6	0.15	No Hit
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTC	6	0.15	No Hit
ACTCAAACTGTGTGACATTTTTGGAATTCTCGGGTGCC	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGG	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGAATTGGAATTCTCGGGT	6	0.15	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATT	6	0.15	No Hit
TATTGCACTTGTCCCGGCCTGAAATGGAATTCTCGGGT	6	0.15	No Hit
TTTGGCAATGGTAGAACTCACACCGGTGGAATTCTCGG	6	0.15	No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGC	6	0.15	No Hit
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCC	6	0.15	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA	6	0.15	No Hit
AATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGCC	6	0.15	No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAA	6	0.15	RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGT	5	0.125	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGC	5	0.125	No Hit
TATCGTCCTTCTGCCCAGCGATCTTGGAATTCTCGGGT	5	0.125	No Hit
TCTTAGATCGATGTGGTGCTCCGGAATGGAATTCTCGG	5	0.125	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGG	5	0.125	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGC	5	0.125	No Hit
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGC	5	0.125	No Hit
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTG	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGGTGGAATTCTCGGGTGC	5	0.125	No Hit
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGC	5	0.125	No Hit
ACTCAAACTGGGGGCTCTTTTTTGGAATTCTCGGGTGC	5	0.125	No Hit
TTTTTCATTATTGCTCCTGACCTGGAATTCTCGGGTGC	5	0.125	No Hit
ATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTTGGA	5	0.125	No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGC	5	0.125	No Hit
AGGTCAGAGGTCGATCCTGGGCTGGAATTCTCGGGTGC	5	0.125	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGTG	5	0.125	No Hit
TCGAGGAGCTCACAGTCTAGTATGGAATTCTCGGGTGC	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACACCGATGGAATTCTCGG	5	0.125	No Hit
GTCTATGATGATCCTATCCCGAACCTGAATTGGAATTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.175	0.0	0.0	0.0
2	0.0	0.175	0.0	0.0	0.0
3	0.0	0.175	0.0	0.0	0.0
4	0.0	0.175	0.0	0.0	0.0
5	0.0	0.175	0.0	0.0	0.0
6	0.0	0.2	0.0	0.0	0.0
7	0.0	0.25	0.0	0.0	0.0
8	0.0	0.3	0.0	0.0	0.0
9	0.0	0.4	0.0	0.0	0.0
10	0.0	0.425	0.0	0.0	0.0
11	0.0	0.55	0.0	0.0	0.0
12	0.0	0.825	0.0	0.0	0.0
13	0.0	1.1	0.0	0.0	0.0
14	0.0	1.475	0.0	0.0	0.0
15	0.0	1.975	0.0	0.0	0.0
16	0.0	2.4	0.0	0.0	0.0
17	0.0	2.725	0.0	0.0	0.0
18	0.0	3.75	0.0	0.0	0.0
19	0.0	4.625	0.0	0.0	0.0
20	0.0	5.2	0.0	0.0	0.0
21	0.0	7.35	0.0	0.0	0.0
22	0.0	13.7	0.0	0.0	0.0
23	0.0	37.9	0.0	0.0	0.0
24	0.0	51.375	0.0	0.0	0.0
25	0.0	58.575	0.0	0.0	0.0
26	0.0	61.9	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGA	20	0.0033271667	32.0	13
CCTGTTG	35	1.2459041E-6	32.0	18
CGCCTGG	20	0.0033271667	32.0	6
CTGGGAA	20	0.0033271667	32.0	9
CCGCCTG	20	0.0033271667	32.0	5
ACCGCCT	20	0.0033271667	32.0	4
TTGAGTC	20	0.0033271667	32.0	16
TATTGCA	75	0.0	32.0	1
GAGTCTT	20	0.0033271667	32.0	18
GCTACTA	20	0.0033271667	32.0	6
ACTTTTG	20	0.0033271667	32.0	12
AGTGCTA	20	0.0033271667	32.0	3
GCTGTGG	20	0.0033271667	32.0	20
GACCGCC	20	0.0033271667	32.0	3
GTCTTGG	20	0.0033271667	32.0	20
TGCTACT	20	0.0033271667	32.0	5
AAGTGCT	20	0.0033271667	32.0	2
AGACCGC	20	0.0033271667	32.0	2
AGTCTTG	20	0.0033271667	32.0	19
AAAGTGC	30	1.7408885E-5	32.0	1
>>END_MODULE
SRR13079631 read2 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079631_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.872	32.0	32.0	32.0	32.0	32.0
2	30.92425	32.0	32.0	32.0	32.0	32.0
3	30.901	32.0	32.0	32.0	32.0	32.0
4	30.938	32.0	32.0	32.0	32.0	32.0
5	30.9915	32.0	32.0	32.0	32.0	32.0
6	34.28425	36.0	36.0	36.0	32.0	36.0
7	34.36875	36.0	36.0	36.0	32.0	36.0
8	34.23125	36.0	36.0	36.0	32.0	36.0
9	34.26425	36.0	36.0	36.0	32.0	36.0
10	34.1185	36.0	36.0	36.0	32.0	36.0
11	34.1125	36.0	36.0	36.0	32.0	36.0
12	34.0935	36.0	36.0	36.0	32.0	36.0
13	34.133	36.0	36.0	36.0	32.0	36.0
14	34.222	36.0	36.0	36.0	32.0	36.0
15	34.194	36.0	36.0	36.0	32.0	36.0
16	34.309	36.0	36.0	36.0	32.0	36.0
17	34.2115	36.0	36.0	36.0	32.0	36.0
18	34.09325	36.0	36.0	36.0	32.0	36.0
19	33.91525	36.0	36.0	36.0	32.0	36.0
20	34.1365	36.0	36.0	36.0	32.0	36.0
21	34.0125	36.0	36.0	36.0	32.0	36.0
22	33.93875	36.0	36.0	36.0	32.0	36.0
23	34.116	36.0	36.0	36.0	32.0	36.0
24	33.7925	36.0	36.0	36.0	32.0	36.0
25	34.02275	36.0	36.0	36.0	32.0	36.0
26	34.01325	36.0	36.0	36.0	32.0	36.0
27	34.09825	36.0	36.0	36.0	32.0	36.0
28	33.83175	36.0	36.0	36.0	32.0	36.0
29	33.984	36.0	36.0	36.0	32.0	36.0
30	33.934	36.0	36.0	36.0	32.0	36.0
31	33.80275	36.0	36.0	36.0	32.0	36.0
32	33.853	36.0	36.0	36.0	32.0	36.0
33	33.94575	36.0	36.0	36.0	32.0	36.0
34	34.102	36.0	36.0	36.0	32.0	36.0
35	34.0465	36.0	36.0	36.0	32.0	36.0
36	33.73675	36.0	36.0	36.0	32.0	36.0
37	33.9475	36.0	36.0	36.0	32.0	36.0
38	32.721	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	2.0
8	2.0
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	2.0
18	2.0
19	2.0
20	2.0
21	12.0
22	12.0
23	15.0
24	22.0
25	31.0
26	53.0
27	87.0
28	69.0
29	91.0
30	130.0
31	204.0
32	231.0
33	366.0
34	845.0
35	1813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.067067067067068	45.6956956956957	22.8978978978979	14.339339339339338
2	32.125	22.675	14.249999999999998	30.95
3	21.3	41.3	19.125	18.275
4	33.050000000000004	17.65	13.625000000000002	35.675000000000004
5	32.025	27.075	21.5	19.400000000000002
6	32.375	17.75	16.25	33.625
7	26.144608456342254	26.695021265949464	13.009757317988491	34.15061295971979
8	33.00800800800801	26.75175175175175	16.166166166166164	24.074074074074073
9	30.345518277416122	27.86680020030045	20.355533299949926	21.4321482223335
10	31.738476953907817	20.59118236472946	18.512024048096194	29.158316633266534
11	28.1203007518797	27.343358395989974	19.69924812030075	24.837092731829575
12	23.552994237033325	21.072412929090454	17.38912553244801	37.98546730142821
13	18.767226259082936	36.18140816837885	14.65798045602606	30.39338511651215
14	25.64552519428428	30.859864627726246	16.796189521183255	26.698420656806217
15	29.172932330827066	23.458646616541355	21.553884711779446	25.81453634085213
16	27.493734335839598	24.260651629072683	30.87719298245614	17.36842105263158
17	36.2929520943065	16.904941058439928	28.768497617256084	18.03360922999749
18	17.790027562014533	16.837885241794037	25.60761713856176	39.76447005762967
19	18.300325896214588	36.42516921534219	20.506392579593882	24.768112308849336
20	19.9899673940306	30.198143967895664	25.332330072736394	24.47955856533735
21	15.1089952392884	25.256827862691054	41.393134552743675	18.241042345276874
22	16.963167126033575	36.88298672012027	28.96517163618141	17.188674517664744
23	36.233701103309926	20.611835506519558	23.520561685055167	19.633901705115345
24	23.678276121272866	40.86695063893761	18.36632422951641	17.088449010273116
25	19.32330827067669	26.842105263157894	35.88972431077694	17.94486215538847
26	20.776942355889723	19.749373433583962	23.909774436090224	35.56390977443609
27	41.23401053423627	13.368447454226235	21.8459994983697	23.551542513167796
28	31.284495735072753	9.081786251881585	34.771700953336676	24.862017059708982
29	29.427997992975413	9.433015554440543	26.39237330657301	34.74661314601104
30	46.0863020572002	11.063723030607125	17.611640742599096	25.23833416959358
31	51.27947817360763	14.450577019568488	15.37882589061716	18.89111891620672
32	32.22974667669927	32.932029094557315	12.39026837220968	22.447955856533735
33	25.71500250878073	20.79779227295534	17.887606623181135	35.59959859508279
34	24.03411941796287	15.429001505268438	35.77521324636227	24.761665830406425
35	40.727728983688834	15.031367628607278	28.48180677540778	15.759096612296112
36	26.919217260411436	19.41796287004516	41.57049673858505	12.092323130958354
37	25.589563472152534	34.194681384846966	27.84746613146011	12.36828901154039
38	40.14049172102358	29.804315102860006	16.608128449573506	13.447064726542902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.5
8	5.0
9	2.5
10	0.0
11	0.0
12	1.5
13	3.0
14	2.0
15	1.0
16	1.0
17	2.0
18	3.0
19	3.0
20	3.0
21	3.0
22	2.5
23	2.0
24	2.0
25	2.0
26	2.0
27	2.0
28	4.5
29	7.0
30	7.0
31	7.0
32	7.0
33	11.0
34	15.0
35	15.0
36	23.0
37	31.0
38	42.0
39	53.0
40	53.0
41	155.5
42	258.0
43	261.5
44	265.0
45	265.0
46	324.5
47	384.0
48	384.0
49	484.0
50	584.0
51	635.0
52	686.0
53	686.0
54	747.5
55	809.0
56	809.0
57	535.5
58	262.0
59	259.5
60	257.0
61	257.0
62	194.5
63	132.0
64	135.0
65	138.0
66	138.0
67	92.0
68	46.0
69	46.0
70	29.5
71	13.0
72	12.5
73	12.0
74	12.0
75	10.5
76	9.0
77	9.0
78	6.5
79	4.0
80	3.0
81	2.0
82	2.0
83	1.5
84	1.0
85	1.0
86	1.5
87	2.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.075
8	0.1
9	0.15
10	0.2
11	0.25
12	0.22499999999999998
13	0.22499999999999998
14	0.27499999999999997
15	0.25
16	0.25
17	0.325
18	0.22499999999999998
19	0.27499999999999997
20	0.325
21	0.22499999999999998
22	0.22499999999999998
23	0.3
24	0.22499999999999998
25	0.25
26	0.25
27	0.325
28	0.35000000000000003
29	0.35000000000000003
30	0.35000000000000003
31	0.35000000000000003
32	0.325
33	0.35000000000000003
34	0.35000000000000003
35	0.375
36	0.35000000000000003
37	0.35000000000000003
38	0.35000000000000003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.08863512778683	38.2
2	6.4709081022294725	5.949999999999999
3	2.8276237085372484	3.9
4	1.7400761283306143	3.2
5	1.1963023382272975	2.75
6	0.4350190320826536	1.2
7	0.5981511691136487	1.925
8	0.5437737901033171	2.0
9	0.27188689505165853	1.125
>10	2.4469820554649266	20.925
>50	0.2175095160413268	7.3
>100	0.1631321370309951	11.525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	234	5.8500000000000005	No Hit
TCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	117	2.9250000000000003	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTA	110	2.75	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATC	85	2.125	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTA	80	2.0	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	76	1.9	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAG	51	1.275	No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAG	48	1.2	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	39	0.975	No Hit
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	38	0.95	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGA	37	0.9249999999999999	No Hit
AGGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAG	36	0.8999999999999999	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAG	33	0.8250000000000001	No Hit
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGA	31	0.775	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAG	31	0.775	No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAG	27	0.675	No Hit
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	25	0.625	No Hit
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	24	0.6	No Hit
GGAGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAG	21	0.525	No Hit
CACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTA	20	0.5	No Hit
AAAGTCTCGCTCTCTGCCCCTCAGATCGTCGGACTGTA	19	0.475	No Hit
GGGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTA	19	0.475	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTG	19	0.475	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGT	19	0.475	No Hit
TCCGGAGCACCACATCGATCTAAGAGATCGTCGGACTG	18	0.44999999999999996	No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGA	18	0.44999999999999996	No Hit
TCAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTA	16	0.4	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGT	16	0.4	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGT	15	0.375	No Hit
ACTCAGAATCACCAGAAATCATCATTACTGATCGTCGG	14	0.35000000000000003	No Hit
AGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAGAA	14	0.35000000000000003	No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAG	14	0.35000000000000003	No Hit
GGGTTAGTGGACCAGGTCACAAAGATCGTCGGACTGTA	13	0.325	No Hit
GGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAG	13	0.325	No Hit
TGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	13	0.325	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGAC	12	0.3	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTA	12	0.3	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTA	12	0.3	No Hit
GTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTA	12	0.3	No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGA	12	0.3	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTG	11	0.27499999999999997	No Hit
AAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATCG	11	0.27499999999999997	No Hit
AGGAGCGAGCGACCAAAGGAACCATAACTGATGATCGT	11	0.27499999999999997	No Hit
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGT	11	0.27499999999999997	No Hit
AGTCAGATCCAATCTGTCATCACTGCTGATCGTCGGAC	11	0.27499999999999997	No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGA	11	0.27499999999999997	Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAG	11	0.27499999999999997	No Hit
GAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAGA	10	0.25	No Hit
CGTACGCTATACGGTCTACTAGATCGTCGGACTGTAGA	10	0.25	No Hit
GGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAGA	10	0.25	No Hit
TCCGGAGCACCACATCGATCTAAGAGGATCGTCGGACT	10	0.25	No Hit
CAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGA	10	0.25	No Hit
TGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTA	9	0.22499999999999998	No Hit
CCGGAGCACCACATCGATCTAAGAGGATCGTCGGACTG	9	0.22499999999999998	No Hit
ACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGAC	9	0.22499999999999998	No Hit
GCATGACGGCCTGCAAGACAGATCGTCGGACTGTAGAA	9	0.22499999999999998	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAG	9	0.22499999999999998	No Hit
CCGGAGCACCACATCGATCTAAGAGATCGTCGGACTGT	8	0.2	No Hit
CTTCAGGTAGTCAGTATTTGTTCGTCATCATGCAGGAT	8	0.2	No Hit
CTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	8	0.2	No Hit
AAAAGTCTCGCTCTCTGCCCCTCAGATCGTCGGACTGT	8	0.2	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGT	8	0.2	No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGT	8	0.2	No Hit
AACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	8	0.2	No Hit
TGGTGCCACCTGTCAGAAATGAACTAACGAGATCGTCG	8	0.2	No Hit
AGGGGTTCACCGAGCAACATTCGATCGTCGGACTGTAG	8	0.2	No Hit
AAAAGCCTAGGTGAGGCCTAACTGTCTGTGATCGTCGG	8	0.2	No Hit
AATCAGAACTTGACATTCAGCACAGAGTTTCTGTCCGC	7	0.17500000000000002	No Hit
GTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGT	7	0.17500000000000002	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
TGCATGACGGCCTGCAAGACAGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
ACACACAAAAGGGAAGCACTTTGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
TTTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTG	7	0.17500000000000002	No Hit
TGGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	7	0.17500000000000002	Illumina RNA PCR Primer (100% over 33bp)
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGGATC	7	0.17500000000000002	No Hit
TTCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAG	6	0.15	No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTGATCGTC	6	0.15	No Hit
ATTCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	6	0.15	No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGAT	6	0.15	No Hit
AGTCAGTATTTGTTCGTCATCATGCGATCGTCGGACTG	6	0.15	No Hit
CCGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACT	6	0.15	No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAA	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACT	6	0.15	No Hit
AAAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATC	5	0.125	No Hit
ATTCAGGTTCGGGATAGGATCATCATAGACGATCGTCG	5	0.125	No Hit
TTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	5	0.125	No Hit
CTTCAGGTAGTCAGTATTTGTTCGTCATCATGCAGGGA	5	0.125	No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGA	5	0.125	No Hit
TTTCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	5	0.125	No Hit
AAGCCTACAGCACCCGATCGTCGGACTGTAGAACTCTG	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	5	0.125	No Hit
GGTCAGGAGCAATAATGAAAAAGATCGTCGGACTGTAG	5	0.125	No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGT	5	0.125	No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAG	5	0.125	No Hit
CCACGGGAACGGATCGTCGGACTGTAGAACTCTGAACG	5	0.125	Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAG	5	0.125	No Hit
TCGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACT	5	0.125	No Hit
ACACCGAGGAGCCCATCATGATGATCGTCGGACTGTAG	5	0.125	No Hit
GACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTA	5	0.125	No Hit
GCCTTCTGACTCCAAGTCCAGTGATCGTCGGACTGTAG	5	0.125	No Hit
CATCAGATAGAGCTAATAAAGATCTTCAGGTAGTCAGT	5	0.125	No Hit
TGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAG	5	0.125	No Hit
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAG	5	0.125	No Hit
CTTTTTCTGTGATCCTGGATCGTCGGACTGTAGAACTC	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAGGCAGAGGCAGGTAGATTGATCGTCGGACTGTAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.25	0.0	0.0
2	0.0	0.0	0.25	0.0	0.0
3	0.0	0.0	0.25	0.0	0.0
4	0.0	0.0	0.25	0.0	0.0
5	0.0	0.0	0.25	0.0	0.0
6	0.0	0.0	0.275	0.0	0.0
7	0.0	0.0	0.325	0.0	0.0
8	0.0	0.0	0.35	0.0	0.0
9	0.0	0.0	0.45	0.0	0.0
10	0.0	0.0	0.45	0.0	0.0
11	0.0	0.0	0.575	0.0	0.0
12	0.0	0.0	0.85	0.0	0.0
13	0.0	0.0	1.1	0.0	0.0
14	0.0	0.0	1.425	0.0	0.0
15	0.0	0.0	1.9	0.0	0.0
16	0.0	0.0	2.3	0.0	0.0
17	0.0	0.0	2.6	0.0	0.0
18	0.0	0.0	3.6	0.0	0.0
19	0.0	0.0	4.4	0.0	0.0
20	0.0	0.0	5.0	0.0	0.0
21	0.0	0.0	7.15	0.0	0.0
22	0.0	0.0	13.55	0.0	0.0
23	0.0	0.0	37.45	0.0	0.0
24	0.0	0.0	50.975	0.0	0.0
25	0.0	0.0	58.05	0.0	0.0
26	0.0	0.0	61.35	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTAGT	20	0.0033271667	32.0	9
AGTAGTA	20	0.0033271667	32.0	10
AGTAGCA	20	0.0033271667	32.0	13
AAAAGTA	20	0.0033271667	32.0	7
TCAAAAG	20	0.0033271667	32.0	5
TAGTAGC	20	0.0033271667	32.0	12
AGACTCA	20	0.0033271667	32.0	1
AAAGTAG	20	0.0033271667	32.0	8
GACTCAA	20	0.0033271667	32.0	2
GTAGTAG	20	0.0033271667	32.0	11
CAAAAGT	20	0.0033271667	32.0	6
ACTCAAA	30	7.034042E-4	26.666666	3
CTCAAAA	30	7.034042E-4	26.666666	4
ACTTTGA	25	0.00990922	25.6	19
CACTTTG	25	0.00990922	25.6	18
TGTAGAA	25	0.00990922	25.6	32
GCACTTT	25	0.00990922	25.6	17
CTTTGAT	25	0.00990922	25.6	20
CAGGCCG	65	7.206836E-9	24.615385	2
GGACAAG	60	1.0250187E-7	24.0	9
>>END_MODULE
Read 760496 spots for SRR13079631.sra
Written 760496 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
Read 760490 spots for SRR13079631.sra
Written 760490 spots for SRR13079631.sra
SRR ids: ['SRR13079631.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xsgl952h
SRR13079631.sra spots: 9125886
blocks: [[1, 760490], [760491, 1520980], [1520981, 2281470], [2281471, 3041960], [3041961, 3802450], [3802451, 4562940], [4562941, 5323430], [5323431, 6083920], [6083921, 6844410], [6844411, 7604900], [7604901, 8365390], [8365391, 9125886]]
SRR13079631 file size 1058358
SRR13079631 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR13079631 SRR13079631_1.fastq SRR13079631_2.fastq
Input file:	SRR13079631_1.fastq
Paired file:	SRR13079631_2.fastq
trimmed:	SRR13079631-trimmed-pair1.fastq, SRR13079631-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:30:40 2021 >> started

Tue Apr 20 16:30:48 2021 >> done (7.897s)
9125886 read pairs processed; of these:
   4494 ( 0.05%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
9121392 (99.95%) read pairs available; of these:
   5400 ( 0.06%) trimmed read pairs available after processing
9115992 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 27	      6	  0.00%
 28	    159	  0.00%
 29	    647	  0.01%
 30	    182	  0.00%
 31	    540	  0.01%
 32	    511	  0.01%
 33	    160	  0.00%
 34	    416	  0.00%
 35	    108	  0.00%
 36	    844	  0.01%
 37	   1827	  0.02%
 38	9115992	 99.94%
9121392 reads passed initial QC


criterion=sequence-density
sequence-density=69.84
sequence-density-rank=1
fanout-score=15.83
fanout-score-rank=1
prefix-density=72.84
prefix-fanout=15.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG


criterion=fanout-score
sequence-density=69.84
sequence-density-rank=1
fanout-score=15.83
fanout-score-rank=1
prefix-density=72.84
prefix-fanout=15.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG


criterion=sequence-density
sequence-density=67.96
sequence-density-rank=1
fanout-score=16.81
fanout-score-rank=1
prefix-density=71.04
prefix-fanout=16.1
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG


criterion=fanout-score
sequence-density=67.96
sequence-density-rank=1
fanout-score=16.81
fanout-score-rank=1
prefix-density=71.04
prefix-fanout=16.1
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG -y GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG -o SRR13079631 SRR13079631_1.fastq SRR13079631_2.fastq
Input file:	SRR13079631_1.fastq
Paired file:	SRR13079631_2.fastq
trimmed:	SRR13079631-trimmed-pair1.fastq, SRR13079631-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG
-- paired 3' end adapter sequence (-y):	GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:31:48 2021 >> started

Tue Apr 20 16:31:56 2021 >> done (7.988s)
8857004 read pairs processed; of these:
1443852 (16.30%) short read pairs filtered out after trimming by size control
 309069 ( 3.49%) empty read pairs filtered out after trimming by size control
7104083 (80.21%) read pairs available; of these:
6605967 (92.99%) trimmed read pairs available after processing
 498116 ( 7.01%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 102264	  1.44%
 19	  68764	  0.97%
 20	 173991	  2.45%
 21	 509606	  7.17%
 22	1816188	 25.57%
 23	 968579	 13.63%
 24	 539032	  7.59%
 25	 276308	  3.89%
 26	 173204	  2.44%
 27	 160794	  2.26%
 28	 194209	  2.73%
 29	 130944	  1.84%
 30	 117563	  1.65%
 31	  96654	  1.36%
 32	 162225	  2.28%
 33	 402461	  5.67%
 34	 455202	  6.41%
 35	 114412	  1.61%
 36	  91682	  1.29%
 37	  53380	  0.75%
 38	 496621	  6.99%


criterion=sequence-density
sequence-density=12.41
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=TATTGCACTTGTCCCGGCCTGTAAA


criterion=fanout-score
sequence-density=0.50
sequence-density-rank=18
fanout-score=34.72
fanout-score-rank=1
prefix-density=14.01
prefix-fanout=1.2
sequence=GTCCCGGCCTGA


criterion=sequence-density
sequence-density=2.83
sequence-density-rank=1
fanout-score=4.38
fanout-score-rank=2
prefix-density=0.10
prefix-fanout=4.4
sequence=AGCCAAGCTCAGACGGATCCGA


criterion=fanout-score
sequence-density=0.43
sequence-density-rank=10
fanout-score=14.55
fanout-score-rank=1
prefix-density=0.45
prefix-fanout=13.8
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAG
SRR13079631 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:32:47
                             Started mapping on |	Apr 20 16:32:48
                                    Finished on |	Apr 20 16:33:33
       Mapping speed, Million of reads per hour |	589.48

                          Number of input reads |	7368471
                      Average input read length |	53
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4790192
                        Uniquely mapped reads % |	65.01%
                          Average mapped length |	47.39
                       Number of splices: Total |	13425
            Number of splices: Annotated (sjdb) |	6009
                       Number of splices: GT/AG |	11235
                       Number of splices: GC/AG |	567
                       Number of splices: AT/AC |	18
               Number of splices: Non-canonical |	1605
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.24
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	576308
             % of reads mapped to multiple loci |	7.82%
        Number of reads mapped to too many loci |	64238
             % of reads mapped to too many loci |	0.87%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	21.90%
                     % of reads unmapped: other |	4.40%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2001971	2001971	2001971
N_multimapping	576308	576308	576308
N_noFeature	561641	640122	3469385
N_ambiguous	2366609	1268792	205193
UnstrandedReadsAssigned:1861942 PositiveStrandReadsAssigned:2881278 NegativeStrandReadsAssigned:1115614
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13079631 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079631-trimmed-pair1.fastq
                             SRR13079631-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 7,368,471 reads, 1,870,986 reads pseudoaligned
[quant] estimated average fragment length: 27.6429
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,099 rounds

 109283 SRR13079631.ke.tsv
  52636 SRR13079631.se.tsv
 161919 total
==> SRR13079631.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079631.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079631 completed mapping pipeline successfully
