Starting /dee2/code/volunteer_pipeline.sh SRR13079629
    current disk space = 414466662400
    free memory = 73742360 
SRR13079629 SRAfilesize
f03956d4b58a7794b6c4f336af0075cc  SRR13079629.sra
SRR13079629.sra file validated
SRR13079629 is paired end
SRR13079629 is conventional basespace
SRR13079629 read1 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079629_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0065	32.0	32.0	32.0	32.0	32.0
2	31.41875	32.0	32.0	32.0	32.0	32.0
3	31.5215	32.0	32.0	32.0	32.0	32.0
4	31.4575	32.0	32.0	32.0	32.0	32.0
5	31.27875	32.0	32.0	32.0	32.0	32.0
6	34.7425	36.0	36.0	36.0	32.0	36.0
7	34.544	36.0	36.0	36.0	32.0	36.0
8	34.76975	36.0	36.0	36.0	32.0	36.0
9	34.731	36.0	36.0	36.0	32.0	36.0
10	34.7285	36.0	36.0	36.0	32.0	36.0
11	34.75875	36.0	36.0	36.0	32.0	36.0
12	34.816	36.0	36.0	36.0	32.0	36.0
13	34.757	36.0	36.0	36.0	32.0	36.0
14	34.888	36.0	36.0	36.0	32.0	36.0
15	34.80675	36.0	36.0	36.0	32.0	36.0
16	34.60925	36.0	36.0	36.0	32.0	36.0
17	34.94775	36.0	36.0	36.0	36.0	36.0
18	34.828	36.0	36.0	36.0	32.0	36.0
19	34.821	36.0	36.0	36.0	32.0	36.0
20	34.851	36.0	36.0	36.0	32.0	36.0
21	34.89125	36.0	36.0	36.0	32.0	36.0
22	34.78275	36.0	36.0	36.0	32.0	36.0
23	34.5745	36.0	36.0	36.0	32.0	36.0
24	34.95225	36.0	36.0	36.0	32.0	36.0
25	34.5065	36.0	36.0	36.0	32.0	36.0
26	34.68875	36.0	36.0	36.0	32.0	36.0
27	34.50625	36.0	36.0	36.0	32.0	36.0
28	34.498	36.0	36.0	36.0	32.0	36.0
29	34.6375	36.0	36.0	36.0	32.0	36.0
30	34.63575	36.0	36.0	36.0	32.0	36.0
31	34.51925	36.0	36.0	36.0	32.0	36.0
32	34.41625	36.0	36.0	36.0	32.0	36.0
33	34.37125	36.0	36.0	36.0	32.0	36.0
34	34.558	36.0	36.0	36.0	32.0	36.0
35	34.45775	36.0	36.0	36.0	32.0	36.0
36	34.51175	36.0	36.0	36.0	32.0	36.0
37	34.50125	36.0	36.0	36.0	32.0	36.0
38	34.497	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	5.0
24	14.0
25	19.0
26	13.0
27	44.0
28	59.0
29	72.0
30	119.0
31	142.0
32	193.0
33	315.0
34	779.0
35	2222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.425	22.75	30.325000000000003	31.5
2	25.4	35.825	16.0	22.775000000000002
3	22.400000000000002	22.3	29.45	25.85
4	29.599999999999998	17.775	28.000000000000004	24.625
5	35.525	30.425	17.775	16.275000000000002
6	25.281884239538964	19.443748433976445	21.322976697569533	33.95139062891506
7	22.025	29.099999999999998	23.05	25.825
8	19.525000000000002	21.3	23.95	35.225
9	14.825	20.974999999999998	34.175	30.025000000000002
10	31.424999999999997	16.6	25.775	26.200000000000003
11	52.525	17.075000000000003	14.95	15.45
12	24.625	27.975	33.1	14.299999999999999
13	31.974999999999998	17.575	26.025	24.425
14	29.075	17.675	24.6	28.65
15	25.174999999999997	26.3	23.375	25.15
16	40.949999999999996	18.75	22.725	17.575
17	31.424999999999997	25.924999999999997	22.5	20.150000000000002
18	20.150000000000002	21.975	22.45	35.425000000000004
19	27.375	24.4	18.95	29.275000000000002
20	25.724999999999998	15.950000000000001	43.325	15.0
21	35.699999999999996	13.350000000000001	25.825	25.124999999999996
22	26.55	19.75	30.45	23.25
23	22.975	16.75	37.2	23.075000000000003
24	49.7	10.875	20.974999999999998	18.45
25	46.125	22.8	16.950000000000003	14.124999999999998
26	32.574999999999996	30.625000000000004	14.975	21.825
27	26.450000000000003	34.300000000000004	18.875	20.375
28	18.35	24.6	39.6	17.45
29	29.45	17.974999999999998	37.675	14.899999999999999
30	18.175	12.675	29.65	39.5
31	18.2	12.375	39.825	29.599999999999998
32	25.1	19.35	22.925	32.625
33	34.9	23.525	20.9	20.674999999999997
34	45.2	16.35	23.325000000000003	15.125
35	50.925	10.225	26.3	12.55
36	42.025	15.125	30.55	12.3
37	41.6	18.825	24.55	15.024999999999999
38	24.4	21.2	16.875	37.525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	1.0
22	1.0
23	1.0
24	1.0
25	3.5
26	6.0
27	6.0
28	7.0
29	8.0
30	14.5
31	21.0
32	21.0
33	29.5
34	38.0
35	38.0
36	52.0
37	66.0
38	73.5
39	81.0
40	81.0
41	163.0
42	245.0
43	236.5
44	228.0
45	228.0
46	284.0
47	340.0
48	340.0
49	382.0
50	424.0
51	514.0
52	604.0
53	604.0
54	563.5
55	523.0
56	523.0
57	444.5
58	366.0
59	462.0
60	558.0
61	558.0
62	431.0
63	304.0
64	198.5
65	93.0
66	93.0
67	58.5
68	24.0
69	24.0
70	22.5
71	21.0
72	17.5
73	14.0
74	14.0
75	11.0
76	8.0
77	8.0
78	8.5
79	9.0
80	6.0
81	3.0
82	3.0
83	2.5
84	2.0
85	2.0
86	2.0
87	2.0
88	3.5
89	5.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	1.5
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.22499999999999998
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.20757825370676	37.425000000000004
2	7.5233388248215265	6.8500000000000005
3	2.416254805052169	3.3000000000000003
4	1.8671059857221308	3.4000000000000004
5	1.2081274025260844	2.75
6	0.8237232289950577	2.25
7	0.5491488193300385	1.7500000000000002
8	0.5491488193300385	2.0
9	0.16474464579901155	0.675
>10	2.14168039538715	16.5
>50	0.3844041735310269	13.075000000000001
>100	0.16474464579901155	10.025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAAC	164	4.1000000000000005	RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGC	123	3.075	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAA	114	2.85	No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGC	97	2.4250000000000003	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTC	80	2.0	RNA PCR Primer, Index 15 (100% over 38bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA	73	1.825	No Hit
AAAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTG	70	1.7500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTA	70	1.7500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA	68	1.7000000000000002	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTG	65	1.625	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGC	48	1.2	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACT	42	1.05	RNA PCR Primer, Index 1 (100% over 25bp)
CATCAAAGTGGAGGCCCTCTCTTGGAATTCTCGGGTGC	25	0.625	No Hit
CCTGCATGATGACGAACAAATACTGACTTGGAATTCTC	24	0.6	No Hit
TCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGGG	24	0.6	No Hit
TTGTGGCAACAATGGCACGGCAAGGGGATCCAGTCCTT	24	0.6	No Hit
CTCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGG	24	0.6	No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGT	22	0.5499999999999999	No Hit
AAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTGC	22	0.5499999999999999	No Hit
ACTCAAACTGGGGGCTCTTTTTGGAATTCTCGGGTGCC	22	0.5499999999999999	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGT	21	0.525	No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGC	20	0.5	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGC	20	0.5	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGC	17	0.42500000000000004	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGC	16	0.4	No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 23bp)
CACAAATGATGAACCTTTTGACGGGCGGACAGAAACTC	14	0.35000000000000003	No Hit
CATCAAAGTGGAGGCCCTCTCTTTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
CTGCATGATGACGAACAAATACTGACTTGGAATTCTCG	13	0.325	No Hit
ACTCAAACTGGGGGCTCTTTTGTGGAATTCTCGGGTGC	13	0.325	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTGGAATTCTCGG	13	0.325	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGC	13	0.325	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGAT	13	0.325	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTGGAA	12	0.3	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGG	11	0.27499999999999997	No Hit
TCTTAGATCGATGTGGTGCTCCGGAATGGAATTCTCGG	11	0.27499999999999997	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTTGGA	11	0.27499999999999997	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGC	11	0.27499999999999997	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCC	11	0.27499999999999997	No Hit
GGCAGAGGAGGGCTGTTCTTCCTGGAATTCTCGGGTGC	11	0.27499999999999997	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATG	10	0.25	No Hit
GGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC	10	0.25	No Hit
GGCAGAGGAGGGCTGTTCTTCTGGAATTCTCGGGTGCC	10	0.25	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGT	10	0.25	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGT	10	0.25	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGG	9	0.22499999999999998	No Hit
TTGTGGCAACAATGGCACGGCAAGGGAGCTGTATTTTG	9	0.22499999999999998	No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGT	9	0.22499999999999998	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTTGGAATTCTC	8	0.2	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGT	8	0.2	No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGC	8	0.2	No Hit
CTCTTAGATCGATGTGGTGCTCCGGAATGGAATTCTCG	8	0.2	No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGT	8	0.2	No Hit
AAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGTG	8	0.2	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTTGGAATTCT	8	0.2	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGTGGAAT	8	0.2	No Hit
AGCAGTGATGACAGATTGGATCTGACTTGGAATTCTCG	8	0.2	No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
ATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTTGGAAT	7	0.17500000000000002	No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
AAAGTGCTTCCCTTTTGTGTGTTGGAATTCTCGGGTGC	7	0.17500000000000002	No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
TGAGGTTGGTGTACTGTGTGTGATGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGAT	7	0.17500000000000002	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGA	7	0.17500000000000002	No Hit
AAGACAGATGTTCTGATCAAGGCCCGAGATTTTGGAAT	7	0.17500000000000002	No Hit
TTTGTGACCTGGTCCACTAACCCTGGAATTCTCGGGTG	6	0.15	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGC	6	0.15	No Hit
CTCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGG	6	0.15	No Hit
TATCGTCCTTCTGCCCAGCGATCTTGGAATTCTCGGGT	6	0.15	No Hit
ACTCAAACTGGGGGCTCTTTTGATGGAATTCTCGGGTG	6	0.15	No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGC	6	0.15	No Hit
TTGTGGCAACAATGGCACGGCAAGGGAGCTGTATTTTT	6	0.15	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGT	6	0.15	No Hit
TGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCT	6	0.15	No Hit
TCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGGT	6	0.15	No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGTGGA	6	0.15	No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGA	6	0.15	No Hit
TATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTG	6	0.15	No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGTGG	6	0.15	No Hit
AAATCTACCTGCCTCTGCCTTGGAATTCTCGGGTGCCA	6	0.15	No Hit
ACCTGAATCGCCCTCGTGAAGCCGCGGAACCAAGTTGG	5	0.125	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGG	5	0.125	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTGGAATTCTCG	5	0.125	No Hit
TCTTAGATCGATGTGGTGCTCCGGAAATGGAATTCTCG	5	0.125	No Hit
TATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5	0.125	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA	5	0.125	No Hit
TGAACTGATGATATCATTTCTTTCCCCGTCAGATCGAC	5	0.125	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCC	5	0.125	No Hit
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGC	5	0.125	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGC	5	0.125	No Hit
GTAAATGATGAGATTCCATTGGTCCGTGTTTCTGAACT	5	0.125	No Hit
AGTGCCGCAGAGTTTGTAGTGTTGGAATTCTCGGGTGC	5	0.125	No Hit
CACATTACACGGTCGACCTCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GGCAGAGGAGGGCTGTTCTTCCCTGGAATTCTCGGGTG	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTG	5	0.125	No Hit
TATGGCACTGGTAGAATTCACTTGGAATTCTCGGGTGC	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGAATGGAATTCTCGGGTG	5	0.125	No Hit
ACTCAAACTGGGGGCTCTTTTATGGAATTCTCGGGTGC	5	0.125	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA	5	0.125	No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGGAATTCTCG	5	0.125	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	2.2	0.0	0.0	0.0
2	0.0	2.25	0.0	0.0	0.0
3	0.0	2.275	0.0	0.0	0.0
4	0.0	2.275	0.0	0.0	0.0
5	0.0	2.325	0.0	0.0	0.0
6	0.0	2.45	0.0	0.0	0.0
7	0.0	2.6	0.0	0.0	0.0
8	0.0	2.8	0.0	0.0	0.0
9	0.0	3.05	0.0	0.0	0.0
10	0.0	3.375	0.0	0.0	0.0
11	0.0	3.775	0.0	0.0	0.0
12	0.0	4.5	0.0	0.0	0.0
13	0.0	5.05	0.0	0.0	0.0
14	0.0	6.475	0.0	0.0	0.0
15	0.0	11.225	0.0	0.0	0.0
16	0.0	12.15	0.0	0.0	0.0
17	0.0	12.9	0.0	0.0	0.0
18	0.0	13.725	0.0	0.0	0.0
19	0.0	14.525	0.0	0.0	0.0
20	0.0	15.3	0.0	0.0	0.0
21	0.0	16.775	0.0	0.0	0.0
22	0.0	21.3	0.0	0.0	0.0
23	0.0	37.875	0.0	0.0	0.0
24	0.0	46.575	0.0	0.0	0.0
25	0.0	51.425	0.0	0.0	0.0
26	0.0	54.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	45	6.2191248E-9	32.0	1
CGTTCCC	20	0.0033271667	32.0	4
CGCTGAT	35	1.2459041E-6	32.0	28
TGCACTT	35	1.2459041E-6	32.0	4
TTGTCCC	35	1.2459041E-6	32.0	9
AAACTGG	30	1.7408885E-5	32.0	5
TTGCACT	35	1.2459041E-6	32.0	3
ACTTGTC	35	1.2459041E-6	32.0	7
GCGACCT	45	6.2191248E-9	32.0	2
GCGACCC	40	8.843199E-8	32.0	22
CCACGTT	20	0.0033271667	32.0	1
CACGTTC	20	0.0033271667	32.0	2
AACTGGG	25	2.4147211E-4	32.0	6
ACGTTCC	20	0.0033271667	32.0	3
CTGGGGG	25	2.4147211E-4	32.0	8
TGTCCCG	35	1.2459041E-6	32.0	10
CCCGGCC	35	1.2459041E-6	32.0	13
GTTCCCG	20	0.0033271667	32.0	5
CTCTTTT	25	2.4147211E-4	32.0	15
CCGCTGA	40	8.843199E-8	32.0	27
>>END_MODULE
SRR13079629 read2 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079629_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96675	32.0	32.0	32.0	32.0	32.0
2	30.8205	32.0	32.0	32.0	32.0	32.0
3	30.792	32.0	32.0	32.0	32.0	32.0
4	30.9755	32.0	32.0	32.0	32.0	32.0
5	31.0305	32.0	32.0	32.0	32.0	32.0
6	34.3815	36.0	36.0	36.0	32.0	36.0
7	34.354	36.0	36.0	36.0	32.0	36.0
8	34.1805	36.0	36.0	36.0	32.0	36.0
9	34.133	36.0	36.0	36.0	32.0	36.0
10	34.19375	36.0	36.0	36.0	32.0	36.0
11	34.1405	36.0	36.0	36.0	32.0	36.0
12	34.11925	36.0	36.0	36.0	32.0	36.0
13	33.9735	36.0	36.0	36.0	32.0	36.0
14	34.09725	36.0	36.0	36.0	32.0	36.0
15	34.247	36.0	36.0	36.0	32.0	36.0
16	34.07075	36.0	36.0	36.0	32.0	36.0
17	34.212	36.0	36.0	36.0	32.0	36.0
18	33.9165	36.0	36.0	36.0	32.0	36.0
19	34.16825	36.0	36.0	36.0	32.0	36.0
20	33.9215	36.0	36.0	36.0	32.0	36.0
21	34.10125	36.0	36.0	36.0	32.0	36.0
22	33.96625	36.0	36.0	36.0	32.0	36.0
23	34.0795	36.0	36.0	36.0	32.0	36.0
24	33.601	36.0	36.0	36.0	27.0	36.0
25	33.89175	36.0	36.0	36.0	32.0	36.0
26	34.075	36.0	36.0	36.0	32.0	36.0
27	33.96375	36.0	36.0	36.0	32.0	36.0
28	34.0065	36.0	36.0	36.0	32.0	36.0
29	33.999	36.0	36.0	36.0	32.0	36.0
30	33.86525	36.0	36.0	36.0	32.0	36.0
31	33.84125	36.0	36.0	36.0	32.0	36.0
32	33.6955	36.0	36.0	36.0	32.0	36.0
33	33.8335	36.0	36.0	36.0	32.0	36.0
34	33.9225	36.0	36.0	36.0	32.0	36.0
35	33.8095	36.0	36.0	36.0	32.0	36.0
36	33.85975	36.0	36.0	36.0	32.0	36.0
37	33.89825	36.0	36.0	36.0	32.0	36.0
38	32.979	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	4.0
9	2.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	2.0
18	1.0
19	4.0
20	5.0
21	8.0
22	18.0
23	17.0
24	18.0
25	39.0
26	41.0
27	76.0
28	85.0
29	119.0
30	134.0
31	166.0
32	254.0
33	388.0
34	827.0
35	1790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.85177766649975	37.88182273410115	24.236354531797698	20.030045067601403
2	26.863431715857928	24.68734367183592	12.88144072036018	35.56778389194598
3	19.225	40.25	20.025000000000002	20.5
4	29.725	16.525000000000002	13.4	40.35
5	35.825	24.224999999999998	18.45	21.5
6	36.675000000000004	17.95	17.424999999999997	27.950000000000003
7	32.883220805201304	23.88097024256064	12.903225806451612	30.332583145786447
8	31.639549436795996	29.43679599499374	15.944931163954942	22.97872340425532
9	27.981995498874717	29.232308077019255	21.980495123780948	20.80520130032508
10	27.691537305958942	18.97846770155233	19.479218828242363	33.85077616424637
11	30.01002004008016	23.446893787575153	17.334669338677354	29.208416833667332
12	22.294589178356713	20.66633266533066	23.146292585170343	33.892785571142284
13	25.36939644377661	29.526671675432002	14.024542950162786	31.0793889306286
14	27.91280380856928	24.780756702580806	18.94262089701829	28.36381859183162
15	29.93486973947896	24.248496993987974	19.98997995991984	25.826653306613228
16	30.060120240480963	22.645290581162325	27.505010020040082	19.789579158316634
17	32.78195488721805	16.917293233082706	31.47869674185464	18.82205513784461
18	17.405459554219885	17.881292261457553	25.64487853744052	39.06836964688205
19	23.978952643447755	29.31596091205212	22.174893510398398	24.53019293410173
20	22.230576441102755	23.734335839598998	29.949874686716793	24.085213032581454
21	17.40110165247872	21.557336004006007	35.65348022033049	25.38808212318478
22	21.96343601302279	31.0793889306286	28.4748309541698	18.482344102178814
23	36.9832122275119	20.646454522676024	19.86970684039088	22.500626409421198
24	22.108162243365047	39.30896344516775	18.678017025538306	19.90485728592889
25	22.019038076152306	25.751503006012022	30.335671342685373	21.8937875751503
26	21.46793587174349	20.59118236472946	24.749498997995993	33.19138276553106
27	43.820506392579595	15.241915266984208	19.85460015041364	21.082978190022562
28	29.305590373527203	12.760090248182502	37.25244422160942	20.681875156680874
29	30.433692654800705	15.016294810729505	25.04387064427175	29.506141890198045
30	40.66182000501379	14.389571321133115	21.408874404612686	23.539734269240412
31	41.438957132113316	21.809977437954377	17.397844071195788	19.353221358736526
32	28.829280521433944	31.63700175482577	14.489847079468538	25.04387064427175
33	25.056433408577877	20.742412841735643	20.19061951341861	34.01053423626787
34	27.02431687139634	14.76560541489095	34.4447229882176	23.76535472549511
35	35.31477301228994	18.159016804615	24.003009781790823	22.523200401304237
36	25.745800952619703	20.155427425419905	40.51140636751065	13.587365254449738
37	28.07721233391828	29.506141890198045	24.216595638004513	18.200050137879167
38	38.154926046628226	28.77914264226623	17.473050889947356	15.592880421158185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	2.0
12	3.5
13	5.0
14	3.5
15	2.0
16	2.0
17	1.0
18	0.0
19	0.0
20	1.0
21	2.0
22	3.5
23	5.0
24	5.0
25	4.5
26	4.0
27	4.0
28	6.5
29	9.0
30	9.5
31	10.0
32	10.0
33	17.0
34	24.0
35	24.0
36	34.5
37	45.0
38	53.5
39	62.0
40	62.0
41	134.0
42	206.0
43	217.0
44	228.0
45	228.0
46	280.0
47	332.0
48	332.0
49	424.0
50	516.0
51	523.0
52	530.0
53	530.0
54	638.0
55	746.0
56	746.0
57	587.5
58	429.0
59	379.5
60	330.0
61	330.0
62	262.0
63	194.0
64	192.0
65	190.0
66	190.0
67	123.0
68	56.0
69	56.0
70	35.0
71	14.0
72	16.5
73	19.0
74	19.0
75	17.0
76	15.0
77	15.0
78	11.0
79	7.0
80	6.5
81	6.0
82	6.0
83	4.5
84	3.0
85	3.0
86	1.5
87	0.0
88	0.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.125
9	0.025
10	0.15
11	0.2
12	0.2
13	0.17500000000000002
14	0.22499999999999998
15	0.2
16	0.2
17	0.25
18	0.17500000000000002
19	0.22499999999999998
20	0.25
21	0.15
22	0.17500000000000002
23	0.22499999999999998
24	0.15
25	0.2
26	0.2
27	0.27499999999999997
28	0.27499999999999997
29	0.27499999999999997
30	0.27499999999999997
31	0.27499999999999997
32	0.27499999999999997
33	0.325
34	0.27499999999999997
35	0.325
36	0.27499999999999997
37	0.27499999999999997
38	0.27499999999999997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.199999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.78601694915254	39.074999999999996
2	7.36228813559322	6.950000000000001
3	2.2245762711864407	3.15
4	1.641949152542373	3.1
5	0.9533898305084746	2.25
6	1.0063559322033897	2.85
7	0.6355932203389831	2.1
8	0.6355932203389831	2.4
9	0.31779661016949157	1.35
>10	1.9597457627118644	16.2
>50	0.31779661016949157	10.925
>100	0.15889830508474578	9.65
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGA	157	3.925	Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	119	2.9749999999999996	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	110	2.75	No Hit
TCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	99	2.475	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	75	1.875	Illumina RNA PCR Primer (100% over 33bp)
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTA	69	1.725	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATC	67	1.675	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	65	1.625	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTA	62	1.55	No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAG	44	1.0999999999999999	No Hit
TGCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGT	39	0.975	No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAA	39	0.975	Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCGGAGCACCACATCGATCTAAGAGGATCGTCGGACT	25	0.625	No Hit
TCCGGAGCACCACATCGATCTAAGAGATCGTCGGACTG	24	0.6	No Hit
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGA	23	0.575	No Hit
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	22	0.5499999999999999	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAG	22	0.5499999999999999	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGA	21	0.525	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAG	21	0.525	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGT	21	0.525	No Hit
TTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGC	20	0.5	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAG	20	0.5	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAG	18	0.44999999999999996	No Hit
AGGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAG	17	0.42500000000000004	No Hit
AATCAGAACTTGACATTCAGCACAGAGTTTCTGTCCGC	16	0.4	No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAG	15	0.375	No Hit
AAAGGACTGGATCCCCTTGCCGTGCCATTGTTGCCACA	14	0.35000000000000003	No Hit
GTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTA	14	0.35000000000000003	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCAT	14	0.35000000000000003	No Hit
CTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	13	0.325	No Hit
AAAGTCTCGCTCTCTGCCCCTCAGATCGTCGGACTGTA	13	0.325	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTA	13	0.325	No Hit
CCACGGGAACGGATCGTCGGACTGTAGAACTCTGAACG	13	0.325	Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGATCGTCGGACTGTAGAACTCTGAAC	13	0.325	Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGAC	12	0.3	No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAG	12	0.3	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTG	12	0.3	No Hit
GGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAG	12	0.3	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAG	12	0.3	No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTG	12	0.3	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	11	0.27499999999999997	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGT	11	0.27499999999999997	No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAG	10	0.25	No Hit
ACTTGGTTCCGCGGCTTCACGAGGGCGATTCAGGTACG	10	0.25	No Hit
AAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATCG	10	0.25	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGT	10	0.25	No Hit
AAAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATC	9	0.22499999999999998	No Hit
GAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAGA	9	0.22499999999999998	No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAG	9	0.22499999999999998	No Hit
AAATACAGCTCCCTTGCCGTGCCATTGTTGCCACAAGA	9	0.22499999999999998	No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGA	9	0.22499999999999998	No Hit
AAAAGGACTGGATCCCCTTGCCGTGCCATTGTTGCCAC	9	0.22499999999999998	No Hit
CCGGAGCACCACATCGATCTAAGAGATCGTCGGACTGT	8	0.2	No Hit
ACTCAGAATCACCAGAAATCATCATTACTGATCGTCGG	8	0.2	No Hit
AGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGC	8	0.2	No Hit
ATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTCG	8	0.2	No Hit
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAG	8	0.2	No Hit
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	8	0.2	No Hit
ACACACAAAAGGGAAGCACTTTGATCGTCGGACTGTAG	8	0.2	No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGAT	8	0.2	No Hit
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGT	8	0.2	No Hit
AGTCAGATCCAATCTGTCATCACTGCTGATCGTCGGAC	8	0.2	No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTC	8	0.2	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGA	8	0.2	No Hit
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
AAATCTCGGGCCTTGATCAGAACATCTGTCTTGATCGT	7	0.17500000000000002	No Hit
CATCAGATAGAGCTAATAAGATCTTCAGGTAGTCAGTA	7	0.17500000000000002	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
GGGTTAGTGGACCAGGTCACAAAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATC	7	0.17500000000000002	No Hit
TCACACACAGTACACCAACCTCAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
AGGAGCGAGCGACCAAAGGAACCATAACTGATGATCGT	7	0.17500000000000002	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTG	7	0.17500000000000002	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGT	7	0.17500000000000002	No Hit
TTCCGGAGCACCACATCGATCTAAGAGGATCGTCGGAC	7	0.17500000000000002	No Hit
AAAATACAGCTCCCTTGCCGTGCCATTGTTGCCACAAG	6	0.15	No Hit
GCTGTTAGCCCTAGCCCCGCAGATCGTCGGACTGTAGA	6	0.15	No Hit
GCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTC	6	0.15	No Hit
TGGCTCCCCGTCGGGGAATCGAACCCCGGTCTCCCGCG	6	0.15	No Hit
AGATCGCTGGGCAGAAGGACGATAGATCGTCGGACTGT	6	0.15	No Hit
AGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAGAA	6	0.15	No Hit
CACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTA	6	0.15	No Hit
GAACCATAACTGATTTAATGAGCCATTCGCAGTTTCAG	6	0.15	No Hit
TCAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTA	6	0.15	No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGT	6	0.15	No Hit
AGCCCAAAAGGAGAATTCTTTGGATCGTCGGACTGTAG	6	0.15	No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGT	6	0.15	No Hit
AGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAG	6	0.15	No Hit
CCGGAGCACCACATCGATCTAAGAGGATCGTCGGACTG	6	0.15	No Hit
CTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCG	6	0.15	No Hit
AGGGGTTCACCGAGCAACATTCGATCGTCGGACTGTAG	6	0.15	No Hit
CAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGA	6	0.15	No Hit
CAGGCGAGCGTGATGACCACTACACTACGGAAACGATC	6	0.15	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.15	No Hit
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCG	5	0.125	No Hit
CTTCAGGTAGTCAGTATTTGTTCGTCATCATGCAGGAT	5	0.125	No Hit
CGGGAACGTGATCGTCGGACTGTAGAACTCTGAACGTG	5	0.125	Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGTACGCTATACGGTCTACTAGATCGTCGGACTGTAGA	5	0.125	No Hit
AGAGGTCGACCGTGTAATGTGGATCGTCGGACTGTAGA	5	0.125	No Hit
TAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAG	5	0.125	No Hit
CATCGACGCACGAGCCGAGTGATCCACCGCTAAGAGTC	5	0.125	No Hit
TTTCCGGAGCACCACATCGATCTAAGAGATCGTCGGAC	5	0.125	No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGA	5	0.125	No Hit
ACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGAC	5	0.125	No Hit
TAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCG	5	0.125	No Hit
GTAGTGCTTTCTACTTTATGGGGATCGTCGGACTGTAG	5	0.125	No Hit
TCGACGCACGAGCCGAGTGATCCACCGGATCGTCGGAC	5	0.125	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGGATC	5	0.125	No Hit
ACTTGGTTCCGCGGCTTCACGAGGGCGATTCAGGTGAT	5	0.125	No Hit
TTCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	5	0.125	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAG	5	0.125	No Hit
CATCAGACAAAACTGGCAATTAGTATTTGAGATCAACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.15	0.0	0.0
2	0.0	0.0	2.2	0.0	0.0
3	0.0	0.0	2.225	0.0	0.0
4	0.0	0.0	2.225	0.0	0.0
5	0.0	0.0	2.275	0.0	0.0
6	0.0	0.0	2.4	0.0	0.0
7	0.0	0.0	2.55	0.0	0.0
8	0.0	0.0	2.775	0.0	0.0
9	0.0	0.0	3.05	0.0	0.0
10	0.0	0.0	3.35	0.0	0.0
11	0.0	0.0	3.725	0.0	0.0
12	0.0	0.0	4.425	0.0	0.0
13	0.0	0.0	4.925	0.0	0.0
14	0.0	0.0	6.225	0.0	0.0
15	0.0	0.0	10.825	0.0	0.0
16	0.0	0.0	11.75	0.0	0.0
17	0.0	0.0	12.55	0.0	0.0
18	0.0	0.0	13.325	0.0	0.0
19	0.0	0.0	14.15	0.0	0.0
20	0.0	0.0	14.925	0.0	0.0
21	0.0	0.0	16.425	0.0	0.0
22	0.0	0.0	20.675	0.0	0.0
23	0.0	0.0	37.175	0.0	0.0
24	0.0	0.0	45.6	0.0	0.0
25	0.0	0.0	50.45	0.0	0.0
26	0.0	0.0	53.05	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGG	20	0.0033271667	32.0	8
CCACGGG	25	2.4147211E-4	32.0	1
CGTGGGA	20	0.0033271667	32.0	10
GTGGGAT	20	0.0033271667	32.0	11
CGGGAAC	20	0.0033271667	32.0	4
ACGGGAA	25	2.4147211E-4	32.0	3
GGGAACG	20	0.0033271667	32.0	5
TGGGATC	20	0.0033271667	32.0	12
GGAACGT	20	0.0033271667	32.0	6
ACGTGGG	20	0.0033271667	32.0	9
GTCGCGG	40	3.5363937E-6	28.0	28
TCGCGGA	40	3.5363937E-6	28.0	29
CGCCACG	40	3.5363937E-6	28.0	10
TCAGCGG	40	3.5363937E-6	28.0	1
GCCACGT	40	3.5363937E-6	28.0	11
AGGTCGC	40	3.5363937E-6	28.0	26
CAGCGGG	40	3.5363937E-6	28.0	2
GGTCGCG	40	3.5363937E-6	28.0	27
GGGTCGC	35	4.995352E-5	27.428572	6
GTCGCCA	35	4.995352E-5	27.428572	8
>>END_MODULE
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
Read 693859 spots for SRR13079629.sra
Written 693859 spots for SRR13079629.sra
SRR ids: ['SRR13079629.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_up8slqcu
SRR13079629.sra spots: 8326308
blocks: [[1, 693859], [693860, 1387718], [1387719, 2081577], [2081578, 2775436], [2775437, 3469295], [3469296, 4163154], [4163155, 4857013], [4857014, 5550872], [5550873, 6244731], [6244732, 6938590], [6938591, 7632449], [7632450, 8326308]]
SRR13079629 file size 965438
SRR13079629 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR13079629 SRR13079629_1.fastq SRR13079629_2.fastq
Input file:	SRR13079629_1.fastq
Paired file:	SRR13079629_2.fastq
trimmed:	SRR13079629-trimmed-pair1.fastq, SRR13079629-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:30:15 2021 >> started

Tue Apr 20 16:30:24 2021 >> done (8.433s)
8326308 read pairs processed; of these:
   4363 ( 0.05%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
8321945 (99.95%) read pairs available; of these:
   5193 ( 0.06%) trimmed read pairs available after processing
8316752 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 27	      7	  0.00%
 28	    166	  0.00%
 29	    564	  0.01%
 30	    169	  0.00%
 31	    488	  0.01%
 32	    478	  0.01%
 33	    155	  0.00%
 34	    346	  0.00%
 35	    125	  0.00%
 36	    866	  0.01%
 37	   1829	  0.02%
 38	8316752	 99.94%
8321945 reads passed initial QC


criterion=sequence-density
sequence-density=59.53
sequence-density-rank=1
fanout-score=14.11
fanout-score-rank=1
prefix-density=62.10
prefix-fanout=13.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA


criterion=fanout-score
sequence-density=59.53
sequence-density-rank=1
fanout-score=14.11
fanout-score-rank=1
prefix-density=62.10
prefix-fanout=13.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA


criterion=sequence-density
sequence-density=57.59
sequence-density-rank=1
fanout-score=19.45
fanout-score-rank=1
prefix-density=60.22
prefix-fanout=18.6
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG


criterion=fanout-score
sequence-density=57.59
sequence-density-rank=1
fanout-score=19.45
fanout-score-rank=1
prefix-density=60.22
prefix-fanout=18.6
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA -y GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG -o SRR13079629 SRR13079629_1.fastq SRR13079629_2.fastq
Input file:	SRR13079629_1.fastq
Paired file:	SRR13079629_2.fastq
trimmed:	SRR13079629-trimmed-pair1.fastq, SRR13079629-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA
-- paired 3' end adapter sequence (-y):	GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:31:07 2021 >> started

Tue Apr 20 16:31:16 2021 >> done (8.372s)
8039845 read pairs processed; of these:
1415409 (17.60%) short read pairs filtered out after trimming by size control
 299143 ( 3.72%) empty read pairs filtered out after trimming by size control
6325293 (78.67%) read pairs available; of these:
5121851 (80.97%) trimmed read pairs available after processing
1203442 (19.03%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  89177	  1.41%
 19	  64258	  1.02%
 20	 129738	  2.05%
 21	 358538	  5.67%
 22	1217701	 19.25%
 23	 643792	 10.18%
 24	 342357	  5.41%
 25	 214008	  3.38%
 26	 167928	  2.65%
 27	 143780	  2.27%
 28	 153001	  2.42%
 29	  92460	  1.46%
 30	  86794	  1.37%
 31	  88878	  1.41%
 32	 138434	  2.19%
 33	 388051	  6.13%
 34	 445795	  7.05%
 35	 124649	  1.97%
 36	 123966	  1.96%
 37	 110510	  1.75%
 38	1201478	 18.99%


criterion=sequence-density
sequence-density=3.06
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=14
prefix-density=0.00
prefix-fanout=1.0
sequence=TTTGGCAATGGTAGAACTCACACCG


criterion=fanout-score
sequence-density=0.41
sequence-density-rank=23
fanout-score=26.83
fanout-score-rank=1
prefix-density=9.27
prefix-fanout=1.2
sequence=GTCCCGGCCTGA


criterion=sequence-density
sequence-density=2.47
sequence-density-rank=1
fanout-score=2.09
fanout-score-rank=6
prefix-density=0.04
prefix-fanout=2.1
sequence=AGCCAAGCTCAGACGGATCCGA


criterion=fanout-score
sequence-density=0.40
sequence-density-rank=12
fanout-score=15.12
fanout-score-rank=1
prefix-density=0.39
prefix-fanout=15.1
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGA
SRR13079629 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:32:16
                             Started mapping on |	Apr 20 16:32:17
                                    Finished on |	Apr 20 16:33:01
       Mapping speed, Million of reads per hour |	540.60

                          Number of input reads |	6607393
                      Average input read length |	57
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3881762
                        Uniquely mapped reads % |	58.75%
                          Average mapped length |	50.74
                       Number of splices: Total |	15888
            Number of splices: Annotated (sjdb) |	8495
                       Number of splices: GT/AG |	13472
                       Number of splices: GC/AG |	897
                       Number of splices: AT/AC |	25
               Number of splices: Non-canonical |	1494
                      Mismatch rate per base, % |	0.33%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.40
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	521820
             % of reads mapped to multiple loci |	7.90%
        Number of reads mapped to too many loci |	65053
             % of reads mapped to too many loci |	0.98%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	28.22%
                     % of reads unmapped: other |	4.15%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2203814	2203814	2203814
N_multimapping	521820	521820	521820
N_noFeature	664753	721613	2898457
N_ambiguous	1690139	872455	177274
UnstrandedReadsAssigned:1526870 PositiveStrandReadsAssigned:2287694 NegativeStrandReadsAssigned:806031
Dataset is classified unstranded
MeadianReadLen=26 20thPercentileLength=22 echo kmer=19
SRR13079629 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079629-trimmed-pair1.fastq
                             SRR13079629-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 6,607,393 reads, 1,545,306 reads pseudoaligned
[quant] estimated average fragment length: 29.8694
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,000 rounds

 109283 SRR13079629.ke.tsv
  52636 SRR13079629.se.tsv
 161919 total
==> SRR13079629.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079629.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079629 completed mapping pipeline successfully
