Starting /dee2/code/volunteer_pipeline.sh SRR13079635
    current disk space = 7097310664
    free memory = 132151048 
SRR13079635 SRAfilesize
d7555c14226b5308aef267e8ebd06564  SRR13079635.sra
SRR13079635.sra file validated
SRR13079635 is paired end
SRR13079635 is conventional basespace
SRR13079635 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.445	32.0	32.0	32.0	21.0	32.0
2	31.2195	32.0	32.0	32.0	32.0	32.0
3	31.492	32.0	32.0	32.0	32.0	32.0
4	31.47	32.0	32.0	32.0	32.0	32.0
5	31.3	32.0	32.0	32.0	32.0	32.0
6	34.9705	36.0	36.0	36.0	36.0	36.0
7	35.1345	36.0	36.0	36.0	36.0	36.0
8	34.966	36.0	36.0	36.0	36.0	36.0
9	34.85525	36.0	36.0	36.0	32.0	36.0
10	34.81975	36.0	36.0	36.0	32.0	36.0
11	34.87775	36.0	36.0	36.0	36.0	36.0
12	34.957	36.0	36.0	36.0	36.0	36.0
13	34.84025	36.0	36.0	36.0	32.0	36.0
14	34.89675	36.0	36.0	36.0	36.0	36.0
15	34.963	36.0	36.0	36.0	32.0	36.0
16	34.893	36.0	36.0	36.0	32.0	36.0
17	34.8225	36.0	36.0	36.0	32.0	36.0
18	35.0075	36.0	36.0	36.0	36.0	36.0
19	35.18425	36.0	36.0	36.0	36.0	36.0
20	35.0035	36.0	36.0	36.0	36.0	36.0
21	35.08175	36.0	36.0	36.0	36.0	36.0
22	34.92925	36.0	36.0	36.0	36.0	36.0
23	34.72	36.0	36.0	36.0	32.0	36.0
24	34.82025	36.0	36.0	36.0	32.0	36.0
25	34.83375	36.0	36.0	36.0	32.0	36.0
26	34.902	36.0	36.0	36.0	36.0	36.0
27	34.78775	36.0	36.0	36.0	32.0	36.0
28	34.83175	36.0	36.0	36.0	32.0	36.0
29	34.8535	36.0	36.0	36.0	32.0	36.0
30	34.9845	36.0	36.0	36.0	36.0	36.0
31	34.63225	36.0	36.0	36.0	32.0	36.0
32	34.70075	36.0	36.0	36.0	32.0	36.0
33	34.8495	36.0	36.0	36.0	32.0	36.0
34	34.896	36.0	36.0	36.0	36.0	36.0
35	34.8415	36.0	36.0	36.0	32.0	36.0
36	34.75625	36.0	36.0	36.0	32.0	36.0
37	34.75075	36.0	36.0	36.0	32.0	36.0
38	34.74625	36.0	36.0	36.0	32.0	36.0
39	34.7015	36.0	36.0	36.0	32.0	36.0
40	34.7025	36.0	36.0	36.0	32.0	36.0
41	34.37375	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	3.0
24	8.0
25	10.0
26	24.0
27	39.0
28	44.0
29	74.0
30	94.0
31	119.0
32	164.0
33	294.0
34	718.0
35	2407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.325	40.175	24.65	22.85
2	26.75	26.625	22.525000000000002	24.099999999999998
3	21.95	18.125	35.3	24.625
4	37.974999999999994	18.25	17.849999999999998	25.924999999999997
5	25.6	34.475	18.05	21.875
6	25.875875875875877	27.72772772772773	21.046046046046047	25.350350350350347
7	29.475	31.6	20.125	18.8
8	19.575	21.125	21.575	37.724999999999994
9	20.8	24.05	38.3	16.85
10	41.275	21.95	22.625	14.149999999999999
11	40.5	22.025	21.05	16.425
12	32.95	24.75	23.849999999999998	18.45
13	29.2	21.3	35.4	14.099999999999998
14	36.58414603650913	23.280820205051263	25.756439109777446	14.378594648662165
15	20.225	25.4	32.5	21.875
16	26.200000000000003	23.849999999999998	35.875	14.075
17	25.424999999999997	20.3	31.324999999999996	22.95
18	18.025	30.475	34.025	17.474999999999998
19	33.15	22.825	28.7	15.325
20	22.475	22.8	40.300000000000004	14.424999999999999
21	31.674999999999997	17.299999999999997	31.55	19.475
22	25.35	32.975	27.325	14.35
23	28.15	40.0	13.725000000000001	18.125
24	37.075	35.625	16.0	11.3
25	23.549999999999997	40.25	22.3	13.900000000000002
26	19.275000000000002	20.424999999999997	28.475	31.825
27	27.450000000000003	18.925	15.950000000000001	37.675
28	45.125	15.725	16.1	23.05
29	42.475	28.7	13.225000000000001	15.6
30	29.775000000000002	44.125	6.75	19.35
31	35.775	41.85	10.45	11.924999999999999
32	37.225	42.5	7.725	12.55
33	32.324999999999996	43.625	7.875	16.175
34	38.324999999999996	30.775000000000002	7.199999999999999	23.7
35	20.925	35.65	8.450000000000001	34.975
36	18.975	43.275000000000006	8.425	29.325000000000003
37	17.7	37.075	7.775	37.45
38	22.975	38.75	7.75	30.525000000000002
39	31.6	23.075000000000003	9.6	35.725
40	36.275	21.525	21.025	21.175
41	19.075	17.9	34.025	28.999999999999996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	1.0
16	1.0
17	1.0
18	0.5
19	0.0
20	0.0
21	0.0
22	0.0
23	2.0
24	4.0
25	4.0
26	3.5
27	3.0
28	2.5
29	2.0
30	5.5
31	9.0
32	9.0
33	11.5
34	14.0
35	26.0
36	38.0
37	38.0
38	73.5
39	109.0
40	175.5
41	242.0
42	242.0
43	279.0
44	316.0
45	431.0
46	546.0
47	546.0
48	536.5
49	527.0
50	713.0
51	899.0
52	743.0
53	587.0
54	587.0
55	423.5
56	260.0
57	227.0
58	194.0
59	194.0
60	156.5
61	119.0
62	108.5
63	98.0
64	98.0
65	58.5
66	19.0
67	11.0
68	3.0
69	3.0
70	2.0
71	1.0
72	1.0
73	1.0
74	1.0
75	1.0
76	1.0
77	1.0
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.025
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78727357176034	46.175
2	5.89874593590339	6.35
3	2.6474686483975844	4.275
4	1.625638643752903	3.5000000000000004
5	0.5573618207152811	1.5
6	0.5109150023223409	1.6500000000000001
7	0.46446818392940087	1.7500000000000002
8	0.46446818392940087	2.0
9	0.23223409196470043	1.125
>10	1.4398513701811426	13.750000000000002
>50	0.23223409196470043	7.1
>100	0.13934045517882027	10.825
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACTCAAACTGGGGGCTCTTTTAGATCGGAAGAGCACACGTC	153	3.8249999999999997	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGT	152	3.8	No Hit
AGTGCCGCAGAGTTTGTAGTGTAGATCGGAAGAGCACACGT	128	3.2	No Hit
ACTCAAACTGGGGGCTCTTTTGAGATCGGAAGAGCACACGT	73	1.825	No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGT	55	1.375	No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACAC	54	1.35	No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTT	51	1.275	No Hit
AGTGCCGCAGAGTTTGTAGTGAGATCGGAAGAGCACACGTC	51	1.275	No Hit
TATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGT	32	0.8	No Hit
ACTCAAACTGTGTGACATTTTAGATCGGAAGAGCACACGTC	29	0.7250000000000001	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGG	27	0.675	No Hit
ATGGCACTGGTAGAATTCACTGAGATCGGAAGAGCACACGT	26	0.65	No Hit
TTTGGCAATGGTAGAACTCACACCAGATCGGAAGAGCACAC	26	0.65	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA	25	0.625	No Hit
AAAGTGCTTCCCTTTTGTGTGTAGATCGGAAGAGCACACGT	23	0.575	No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTT	23	0.575	No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCT	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGATTCTGATGTTGGTCACACTAGATCGGAAGAGCACA	22	0.5499999999999999	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGT	21	0.525	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTAGATCGGAAGAGCACAC	20	0.5	No Hit
ACTCAAACTGGGGGCTCTTTCAGATCGGAAGAGCACACGTC	19	0.475	No Hit
TATGGCACTGGTAGAATTCACTGAGATCGGAAGAGCACACG	19	0.475	No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGT	19	0.475	No Hit
CCTGCATGATGACGAACAAATACTGACTAGATCGGAAGAGC	16	0.4	No Hit
AGTGCCGCAGAGTTTGTAGTGCAGATCGGAAGAGCACACGT	16	0.4	No Hit
ACTCAAACTGTGTGACATTTAGATCGGAAGAGCACACGTCT	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGCAATGGTAGAACTCACACCGAGATCGGAAGAGCACA	14	0.35000000000000003	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCG	14	0.35000000000000003	No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACAC	13	0.325	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAA	12	0.3	No Hit
CATCAAAGTGGAGGCCCTCTCTAGATCGGAAGAGCACACGT	11	0.27499999999999997	No Hit
ACTCAAACTGGGGGCTCTTTAGATCGGAAGAGCACACGTCT	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCAAACTGGGGGCTCTTTTAAGATCGGAAGAGCACACGT	11	0.27499999999999997	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTA	11	0.27499999999999997	No Hit
AGTGCCGCAGAGTTTGTAGTAAGATCGGAAGAGCACACGTC	11	0.27499999999999997	No Hit
CTGCATGATGACGAACAAATACTGACTAGATCGGAAGAGCA	11	0.27499999999999997	No Hit
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGT	10	0.25	No Hit
AAGTGCCGCCAGGTTTTGAGTGTAGATCGGAAGAGCACACG	10	0.25	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGA	10	0.25	No Hit
AAGGGATTCTGATGTTGGTCACACTCAGATCGGAAGAGCAC	9	0.22499999999999998	No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
ATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
TGGTAGACTATGGAACGTAGGAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGAT	9	0.22499999999999998	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAG	8	0.2	No Hit
TGGAAGACTAGTGATTTTGTTGTAGATCGGAAGAGCACACG	8	0.2	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTAGATCGGAAGAG	8	0.2	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAG	8	0.2	No Hit
TGGAAGACTAGTGATTTTGTTGTCAGATCGGAAGAGCACAC	8	0.2	No Hit
AAGTGCTACTACTTTTGAGTCTAGATCGGAAGAGCACACGT	8	0.2	No Hit
TAAAGTGCTTATAGTGCAGGTAGAGATCGGAAGAGCACACG	8	0.2	No Hit
TGACTGTGATGAATCTTACTCTGAGTCACAGATCGGAAGAG	8	0.2	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCAAGATCGGAA	8	0.2	No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACG	8	0.2	No Hit
GAATGTTGCTCGGTGAACCCCTAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
CACATTACACGGTCGACCTCTAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGA	7	0.17500000000000002	No Hit
TGTAAACATCCTACACTCTCAGTAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
AAAGTGCTACTACTTTTGAGTCTAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
ACTCAAACTGGGGGCTCTTTTGTAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
CACCCGTAGAACCGACCTTGCGAGATCGGAAGAGCACACGT	6	0.15	No Hit
TTCAAGTAATCCAGGATAGGCGAGATCGGAAGAGCACACGT	6	0.15	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAG	6	0.15	No Hit
CATCAAAGTGGAGGCCCTCTCTTAGATCGGAAGAGCACACG	6	0.15	No Hit
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGT	6	0.15	No Hit
TGAGGTTGGTGTACTGTGTGTGAAGATCGGAAGAGCACACG	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATC	6	0.15	No Hit
GTGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAAT	6	0.15	No Hit
TTATAAAGCAATGAGACTGATTAGATCGGAAGAGCACACGT	6	0.15	No Hit
TGCTATGATGAAGGCTATGTTGGTAGGGACAACTGAGCTTG	6	0.15	No Hit
ACTCAAACTGGGGGCTCTTTAAGATCGGAAGAGCACACGTC	6	0.15	No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTC	5	0.125	No Hit
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACAC	5	0.125	No Hit
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGT	5	0.125	No Hit
TGGAAGACTAGTGATTTTGTTGAGATCGGAAGAGCACACGT	5	0.125	No Hit
TATGGCACTGGTAGAATTCACCAGATCGGAAGAGCACACGT	5	0.125	No Hit
CATCAAAGTGGAGGCCCTCTCCAGATCGGAAGAGCACACGT	5	0.125	No Hit
TAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGA	5	0.125	No Hit
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACAC	5	0.125	No Hit
CTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCA	5	0.125	No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGG	5	0.125	No Hit
AAAGGACTATTTTTTAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.125	0.0	0.0	0.0	0.0
2	0.125	0.0	0.0	0.0	0.0
3	0.125	0.0	0.0	0.0	0.0
4	0.125	0.0	0.0	0.0	0.0
5	0.125	0.0	0.0	0.0	0.0
6	0.125	0.0	0.0	0.0	0.0
7	0.15	0.0	0.0	0.0	0.0
8	0.175	0.0	0.0	0.0	0.0
9	0.275	0.0	0.0	0.0	0.0
10	0.525	0.0	0.0	0.0	0.0
11	0.8	0.0	0.0	0.0	0.0
12	1.3	0.0	0.0	0.0	0.0
13	2.25	0.0	0.0	0.0	0.0
14	3.05	0.0	0.0	0.0	0.0
15	3.875	0.0	0.0	0.0	0.0
16	4.975	0.0	0.0	0.0	0.0
17	5.775	0.0	0.0	0.0	0.0
18	6.775	0.0	0.0	0.0	0.0
19	7.775	0.0	0.0	0.0	0.0
20	9.075	0.0	0.0	0.0	0.0
21	12.25	0.0	0.0	0.0	0.0
22	25.05	0.0	0.0	0.0	0.0
23	44.65	0.0	0.0	0.0	0.0
24	51.875	0.0	0.0	0.0	0.0
25	58.05	0.0	0.0	0.0	0.0
26	60.5	0.0	0.0	0.0	0.0
27	61.925	0.0	0.0	0.0	0.0
28	63.775	0.0	0.0	0.0	0.0
29	65.85	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTGG	45	2.588422E-9	35.000004	5
TTGTAGT	30	9.417061E-6	35.000004	14
AACTGGG	45	2.588422E-9	35.000004	6
CTGGGGG	45	2.588422E-9	35.000004	8
CTCTTTT	45	2.588422E-9	35.000004	15
TGGGGGC	45	2.588422E-9	35.000004	9
GGGGGCT	45	2.588422E-9	35.000004	10
GGCTCTT	45	2.588422E-9	35.000004	13
TGTAGTG	30	9.417061E-6	35.000004	15
GGGGCTC	45	2.588422E-9	35.000004	11
GCTCTTT	45	2.588422E-9	35.000004	14
ACTGGGG	45	2.588422E-9	35.000004	7
GGGCTCT	45	2.588422E-9	35.000004	12
TGTTGGT	20	0.0021434545	35.0	13
TGGTCAC	20	0.0021434545	35.0	16
GATGTTG	20	0.0021434545	35.0	11
TTCTGAT	20	0.0021434545	35.0	7
GGATTCT	20	0.0021434545	35.0	4
CTGATGT	20	0.0021434545	35.0	9
GGTCACA	20	0.0021434545	35.0	17
>>END_MODULE
SRR13079635 read2 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079635_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8025	32.0	32.0	32.0	32.0	32.0
2	30.7845	32.0	32.0	32.0	32.0	32.0
3	30.57675	32.0	32.0	32.0	32.0	32.0
4	30.61625	32.0	32.0	32.0	32.0	32.0
5	30.51	32.0	32.0	32.0	32.0	32.0
6	33.83525	36.0	36.0	36.0	32.0	36.0
7	33.87625	36.0	36.0	36.0	32.0	36.0
8	33.72525	36.0	36.0	36.0	32.0	36.0
9	33.82425	36.0	36.0	36.0	32.0	36.0
10	34.03775	36.0	36.0	36.0	32.0	36.0
11	33.83925	36.0	36.0	36.0	32.0	36.0
12	33.96925	36.0	36.0	36.0	32.0	36.0
13	34.1655	36.0	36.0	36.0	32.0	36.0
14	33.87825	36.0	36.0	36.0	32.0	36.0
15	33.88575	36.0	36.0	36.0	32.0	36.0
16	34.0425	36.0	36.0	36.0	32.0	36.0
17	34.16675	36.0	36.0	36.0	32.0	36.0
18	34.10225	36.0	36.0	36.0	32.0	36.0
19	34.12275	36.0	36.0	36.0	32.0	36.0
20	34.02125	36.0	36.0	36.0	32.0	36.0
21	34.0965	36.0	36.0	36.0	32.0	36.0
22	34.01225	36.0	36.0	36.0	32.0	36.0
23	34.19525	36.0	36.0	36.0	32.0	36.0
24	33.99575	36.0	36.0	36.0	32.0	36.0
25	33.9755	36.0	36.0	36.0	32.0	36.0
26	34.09875	36.0	36.0	36.0	32.0	36.0
27	34.04525	36.0	36.0	36.0	32.0	36.0
28	33.8895	36.0	36.0	36.0	32.0	36.0
29	34.0465	36.0	36.0	36.0	32.0	36.0
30	33.9485	36.0	36.0	36.0	32.0	36.0
31	33.91475	36.0	36.0	36.0	32.0	36.0
32	34.28425	36.0	36.0	36.0	32.0	36.0
33	34.091	36.0	36.0	36.0	32.0	36.0
34	33.912	36.0	36.0	36.0	32.0	36.0
35	33.9295	36.0	36.0	36.0	32.0	36.0
36	33.906	36.0	36.0	36.0	32.0	36.0
37	33.6085	36.0	36.0	36.0	27.0	36.0
38	33.8075	36.0	36.0	36.0	32.0	36.0
39	33.62425	36.0	36.0	36.0	27.0	36.0
40	33.58425	36.0	36.0	36.0	27.0	36.0
41	32.87775	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	3.0
17	6.0
18	8.0
19	2.0
20	8.0
21	4.0
22	18.0
23	28.0
24	36.0
25	51.0
26	63.0
27	77.0
28	88.0
29	100.0
30	113.0
31	163.0
32	202.0
33	358.0
34	890.0
35	1778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.925	50.349999999999994	14.2	19.525000000000002
2	25.674999999999997	35.15	8.4	30.775000000000002
3	14.549999999999999	41.275	21.725	22.45
4	18.6	34.25	16.950000000000003	30.2
5	22.875	39.074999999999996	21.05	17.0
6	27.500000000000004	28.65	16.05	27.800000000000004
7	20.5	31.05	20.974999999999998	27.474999999999998
8	17.424999999999997	34.050000000000004	17.8	30.725
9	14.549999999999999	30.15	23.375	31.924999999999997
10	12.575	25.124999999999996	28.799999999999997	33.5
11	24.675	19.25	21.425	34.65
12	18.275	19.650000000000002	26.450000000000003	35.625
13	23.3	31.225	17.45	28.025
14	25.55	22.45	28.050000000000004	23.95
15	23.775	17.974999999999998	28.249999999999996	30.0
16	25.724999999999998	22.8	27.950000000000003	23.525
17	27.750000000000004	21.7	31.75	18.8
18	33.300000000000004	17.7	26.174999999999997	22.825
19	25.174999999999997	27.1	17.599999999999998	30.125
20	29.5	25.374999999999996	25.924999999999997	19.2
21	19.325	19.400000000000002	32.25	29.025000000000002
22	25.5	23.025000000000002	34.025	17.45
23	38.125	26.224999999999998	18.975	16.675
24	19.75	41.675000000000004	23.474999999999998	15.1
25	20.025000000000002	17.775	30.5	31.7
26	25.15	17.5	25.05	32.300000000000004
27	33.2	15.15	33.75	17.9
28	23.680920230057513	11.927981995498875	35.98399599899975	28.40710177544386
29	34.45861465366342	12.953238309577394	17.504376094023506	35.08377094273568
30	48.0	19.125	16.85	16.025
31	38.03450862715679	28.33208302075519	13.978494623655912	19.654913728432106
32	25.424999999999997	29.525000000000002	20.1	24.95
33	22.825	24.425	23.125	29.625
34	33.125	16.475	33.45	16.950000000000003
35	31.2	19.8	30.875000000000004	18.125
36	22.725	23.925	39.5	13.850000000000001
37	31.1	33.025	18.775	17.1
38	39.775	31.574999999999996	17.1	11.55
39	20.474999999999998	47.099999999999994	11.95	20.474999999999998
40	20.275000000000002	43.725	13.325000000000001	22.675
41	22.725	23.724999999999998	23.075000000000003	30.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	1.0
5	2.0
6	2.5
7	3.0
8	3.0
9	2.5
10	2.0
11	1.5
12	1.0
13	3.0
14	5.0
15	5.0
16	3.5
17	2.0
18	3.5
19	5.0
20	5.0
21	3.5
22	2.0
23	2.5
24	3.0
25	3.0
26	4.0
27	5.0
28	5.5
29	6.0
30	8.5
31	11.0
32	11.0
33	11.5
34	12.0
35	19.5
36	27.0
37	27.0
38	48.0
39	69.0
40	164.5
41	260.0
42	260.0
43	300.5
44	341.0
45	461.5
46	582.0
47	582.0
48	716.5
49	851.0
50	819.0
51	787.0
52	566.0
53	345.0
54	345.0
55	274.0
56	203.0
57	170.0
58	137.0
59	137.0
60	140.0
61	143.0
62	122.5
63	102.0
64	102.0
65	71.5
66	41.0
67	31.5
68	22.0
69	22.0
70	14.0
71	6.0
72	5.0
73	4.0
74	4.0
75	4.0
76	4.0
77	2.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.5
83	1.0
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	1.0
95	2.0
96	2.0
97	2.0
98	2.0
99	5.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.025
29	0.025
30	0.0
31	0.025
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51535380507343	48.6
2	5.740987983978639	6.45
3	2.7592345349354694	4.65
4	1.2016021361815754	2.7
5	0.6675567423230975	1.875
6	0.4450378282153983	1.5
7	0.3115264797507788	1.225
8	0.5340453938584779	2.4
9	0.22251891410769914	1.125
>10	1.335113484646195	14.124999999999998
>50	0.13351134846461948	4.575
>100	0.13351134846461948	10.775
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	163	4.075	No Hit
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	144	3.5999999999999996	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAAC	124	3.1	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAAC	72	1.7999999999999998	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	56	1.4000000000000001	No Hit
AACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGA	55	1.375	No Hit
CACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAACT	48	1.2	No Hit
GCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	40	1.0	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGAACT	29	0.7250000000000001	No Hit
AGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAAC	27	0.675	No Hit
CAGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAGAAC	26	0.65	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	25	0.625	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGTCGG	25	0.625	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTC	24	0.6	No Hit
ACACACAAAAGGGAAGCACTTTGATCGTCGGACTGTAGAAC	23	0.575	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTAG	22	0.5499999999999999	No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAAC	20	0.5	No Hit
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTC	20	0.5	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	19	0.475	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTAGA	18	0.44999999999999996	No Hit
GCACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAAC	17	0.42500000000000004	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAA	17	0.42500000000000004	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAGAAC	16	0.4	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGACTG	15	0.375	No Hit
AAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGAACTC	14	0.35000000000000003	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATCGT	13	0.325	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	13	0.325	No Hit
AGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	12	0.3	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTAGAA	11	0.27499999999999997	No Hit
CGCACGAGCCGAGTGATCCACCGCTAAGAGTCGATCGTCGG	11	0.27499999999999997	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCG	10	0.25	No Hit
TAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAAC	10	0.25	No Hit
CATCAGAATCGTTGGTATTCATCAACAAGCTCAGTTGTCCC	10	0.25	No Hit
TACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAACT	10	0.25	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGACTGT	10	0.25	No Hit
GAGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTA	10	0.25	No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGA	9	0.22499999999999998	No Hit
AAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACTC	9	0.22499999999999998	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCTACGTTCCATAGTCTACCAGATCGTCGGACTGTAGAACT	9	0.22499999999999998	No Hit
AGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAAC	9	0.22499999999999998	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAGAAC	9	0.22499999999999998	No Hit
ACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGAA	8	0.2	No Hit
ACAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAA	8	0.2	No Hit
AGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAGAACT	8	0.2	No Hit
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGAC	8	0.2	No Hit
ACTCAGAATCACCAGAAATCATCATTACTGATCGTCGGACT	8	0.2	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAGAAC	8	0.2	No Hit
AAATGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGG	8	0.2	No Hit
GACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGA	8	0.2	No Hit
GTGACTCAGAGTAAGATTCATCACAGTCAGATCGTCGGACT	8	0.2	No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	8	0.2	No Hit
AGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCGGA	8	0.2	No Hit
GGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	8	0.2	No Hit
CCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	7	0.17500000000000002	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
ACTTGGTTCCGCGGCTTCACGAGGGCGATTCAGGTACGCAC	7	0.17500000000000002	No Hit
AGGGGTTCACCGAGCAACATTCGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
TGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGTCGG	7	0.17500000000000002	No Hit
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGA	6	0.15	No Hit
ACTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAA	6	0.15	No Hit
TCACACACAGTACACCAACCTCAGATCGTCGGACTGTAGAA	6	0.15	No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	6	0.15	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.15	No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAAC	6	0.15	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	6	0.15	Illumina RNA PCR Primer (100% over 36bp)
CTACCTGCACTATAAGCACTTTAGATCGTCGGACTGTAGAA	6	0.15	No Hit
GATCAGCAAGAGTATTCTCTTCATTTCAGGTCAGTCCCGAA	6	0.15	No Hit
TAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	6	0.15	No Hit
CGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
CAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAGAACT	5	0.125	No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AGAGGTCGACCGTGTAATGTGGATCGTCGGACTGTAGAACT	5	0.125	No Hit
AGTCAGCCAGGAGCAGTTATCGTCAGTTATCGACTCTGACG	5	0.125	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTATGATCGTC	5	0.125	No Hit
ACCAGGTTCCACCCCAGCAGGCGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTAGATCGTCG	5	0.125	No Hit
GGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AGATTGCAGCACCTGAGTTTCGCGTATGGTCACCCACTACA	5	0.125	No Hit
GGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AAAAAATAGTCCTTTGATCGTCGGACTGTAGAACTCTGAAC	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGA	5	0.125	No Hit
TACAGATGGATACCGTGCAATTGATCGTCGGACTGTAGAAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.15	0.0	0.0
2	0.0	0.0	0.15	0.0	0.0
3	0.0	0.0	0.15	0.0	0.0
4	0.0	0.0	0.15	0.0	0.0
5	0.0	0.0	0.15	0.0	0.0
6	0.0	0.0	0.15	0.0	0.0
7	0.0	0.0	0.175	0.0	0.0
8	0.0	0.0	0.2	0.0	0.0
9	0.0	0.0	0.3	0.0	0.0
10	0.0	0.0	0.525	0.0	0.0
11	0.0	0.0	0.775	0.0	0.0
12	0.0	0.0	1.3	0.0	0.0
13	0.0	0.0	2.2	0.0	0.0
14	0.0	0.0	2.975	0.0	0.0
15	0.0	0.0	3.725	0.0	0.0
16	0.0	0.0	4.85	0.0	0.0
17	0.0	0.0	5.625	0.0	0.0
18	0.0	0.0	6.575	0.0	0.0
19	0.0	0.0	7.65	0.0	0.0
20	0.0	0.0	8.85	0.0	0.0
21	0.0	0.0	11.7	0.0	0.0
22	0.0	0.0	24.425	0.0	0.0
23	0.0	0.0	43.375	0.0	0.0
24	0.0	0.0	50.15	0.0	0.0
25	0.0	0.0	56.3	0.0	0.0
26	0.0	0.0	58.6	0.0	0.0
27	0.0	0.0	60.025	0.0	0.0
28	0.0	0.0	61.7	0.0	0.0
29	0.0	0.0	63.65	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCAGTT	35	0.0010258359	25.000002	10
TTTGAGT	45	1.5026014E-4	23.333334	15
GTTTGAG	45	1.5026014E-4	23.333334	14
TTGAGTG	45	1.5026014E-4	23.333334	16
TGAGTGA	45	1.5026014E-4	23.333334	17
TAGAACT	95	2.1827873E-10	22.105265	35
CCCCCAG	40	0.0022346217	21.875	8
CCCCAGT	40	0.0022346217	21.875	9
GCCCCCA	40	0.0022346217	21.875	7
CAGTTTG	50	3.0707463E-4	21.0	12
GCGGCAC	50	3.0707463E-4	21.0	14
CGGCACT	50	3.0707463E-4	21.0	15
AGTTTGA	50	3.0707463E-4	21.0	13
GAGTGAT	60	4.108416E-5	20.416668	18
GTGATCG	60	4.108416E-5	20.416668	20
GTAGAAC	185	0.0	17.972973	35
AGTGATC	70	1.3470645E-4	17.500002	19
TGTAGAA	210	0.0	15.833334	34
TTGATCG	80	3.7450963E-4	15.3125	21
CTGTAGA	240	0.0	13.854168	33
>>END_MODULE
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
Read 143234 spots for SRR13079635.sra
Written 143234 spots for SRR13079635.sra
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SRR ids: ['SRR13079635.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_rlb_2n8q
SRR13079635.sra spots: 9167014
blocks: [[1, 143234], [143235, 286468], [286469, 429702], [429703, 572936], [572937, 716170], [716171, 859404], [859405, 1002638], [1002639, 1145872], [1145873, 1289106], [1289107, 1432340], [1432341, 1575574], [1575575, 1718808], [1718809, 1862042], [1862043, 2005276], [2005277, 2148510], [2148511, 2291744], [2291745, 2434978], [2434979, 2578212], [2578213, 2721446], [2721447, 2864680], [2864681, 3007914], [3007915, 3151148], [3151149, 3294382], [3294383, 3437616], [3437617, 3580850], [3580851, 3724084], [3724085, 3867318], [3867319, 4010552], [4010553, 4153786], [4153787, 4297020], [4297021, 4440254], [4440255, 4583488], [4583489, 4726722], [4726723, 4869956], [4869957, 5013190], [5013191, 5156424], [5156425, 5299658], [5299659, 5442892], [5442893, 5586126], [5586127, 5729360], [5729361, 5872594], [5872595, 6015828], [6015829, 6159062], [6159063, 6302296], [6302297, 6445530], [6445531, 6588764], [6588765, 6731998], [6731999, 6875232], [6875233, 7018466], [7018467, 7161700], [7161701, 7304934], [7304935, 7448168], [7448169, 7591402], [7591403, 7734636], [7734637, 7877870], [7877871, 8021104], [8021105, 8164338], [8164339, 8307572], [8307573, 8450806], [8450807, 8594040], [8594041, 8737274], [8737275, 8880508], [8880509, 9023742], [9023743, 9167014]]
SRR13079635 file size 1116852
SRR13079635 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13079635 SRR13079635_1.fastq SRR13079635_2.fastq
Input file:	SRR13079635_1.fastq
Paired file:	SRR13079635_2.fastq
trimmed:	SRR13079635-trimmed-pair1.fastq, SRR13079635-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:38:26 2021 >> started

Tue Apr 20 16:38:42 2021 >> done (16.467s)
9167014 read pairs processed; of these:
1308015 (14.27%) short read pairs filtered out after trimming by size control
 190808 ( 2.08%) empty read pairs filtered out after trimming by size control
7668191 (83.65%) read pairs available; of these:
6693486 (87.29%) trimmed read pairs available after processing
 974705 (12.71%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 148143	  1.93%
 19	 136339	  1.78%
 20	 313650	  4.09%
 21	1056221	 13.77%
 22	1939659	 25.29%
 23	 709210	  9.25%
 24	 511994	  6.68%
 25	 204215	  2.66%
 26	 120827	  1.58%
 27	 123777	  1.61%
 28	 143400	  1.87%
 29	 118847	  1.55%
 30	 112255	  1.46%
 31	 168566	  2.20%
 32	 187802	  2.45%
 33	 142306	  1.86%
 34	 140801	  1.84%
 35	  98136	  1.28%
 36	  76380	  1.00%
 37	  76765	  1.00%
 38	  61300	  0.80%
 39	  54440	  0.71%
 40	  48453	  0.63%
 41	 974705	 12.71%
7668191 reads passed initial QC


criterion=sequence-density
sequence-density=8.15
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=5
prefix-density=0.01
prefix-fanout=1.0
sequence=ACTCAAACTGGGGGCTCTTTTG


criterion=fanout-score
sequence-density=0.84
sequence-density-rank=12
fanout-score=43.75
fanout-score-rank=1
prefix-density=1.04
prefix-fanout=35.4
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCT


criterion=sequence-density
sequence-density=2.80
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=8
prefix-density=0.00
prefix-fanout=1.0
sequence=ACACTACAAACTCTGCGGCACTTC


criterion=fanout-score
sequence-density=0.70
sequence-density-rank=7
fanout-score=44.20
fanout-score-rank=1
prefix-density=0.82
prefix-fanout=37.6
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x ACTCAAACTGGGGGCTCTTTTG -y ACACTACAAACTCTGCGGCACTTC -o SRR13079635 SRR13079635_1.fastq SRR13079635_2.fastq
Input file:	SRR13079635_1.fastq
Paired file:	SRR13079635_2.fastq
trimmed:	SRR13079635-trimmed-pair1.fastq, SRR13079635-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACTCAAACTGGGGGCTCTTTTG
-- paired 3' end adapter sequence (-y):	ACACTACAAACTCTGCGGCACTTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:39:05 2021 >> started

Tue Apr 20 16:39:14 2021 >> done (9.475s)
5112127 read pairs processed; of these:
   2047 ( 0.04%) short read pairs filtered out after trimming by size control
 656879 (12.85%) empty read pairs filtered out after trimming by size control
4453201 (87.11%) read pairs available; of these:
    115 ( 0.00%) trimmed read pairs available after processing
4453086 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  98185	  2.20%
 19	  89530	  2.01%
 20	 193822	  4.35%
 21	 411086	  9.23%
 22	 973868	 21.87%
 23	 454504	 10.21%
 24	 339355	  7.62%
 25	 135386	  3.04%
 26	  80473	  1.81%
 27	  82516	  1.85%
 28	  95417	  2.14%
 29	  79279	  1.78%
 30	  74735	  1.68%
 31	 112084	  2.52%
 32	 124928	  2.81%
 33	  94757	  2.13%
 34	  94039	  2.11%
 35	  65656	  1.47%
 36	  50692	  1.14%
 37	  51033	  1.15%
 38	  40377	  0.91%
 39	  36127	  0.81%
 40	  32186	  0.72%
 41	 643166	 14.44%


criterion=sequence-density
sequence-density=2.80
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=5
prefix-density=0.00
prefix-fanout=1.0
sequence=TTCAAGTAATCCAGGATAGGCTA


criterion=fanout-score
sequence-density=1.09
sequence-density-rank=10
fanout-score=49.24
fanout-score-rank=1
prefix-density=1.33
prefix-fanout=40.3
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCT


criterion=sequence-density
sequence-density=1.98
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=10
prefix-density=0.00
prefix-fanout=1.0
sequence=AGCCTATCCTGGATTACTTGAAGATCGTCG


criterion=fanout-score
sequence-density=0.82
sequence-density-rank=5
fanout-score=51.35
fanout-score-rank=1
prefix-density=1.00
prefix-fanout=42.0
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC
SRR13079635 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:40:22
                             Started mapping on |	Apr 20 16:40:22
                                    Finished on |	Apr 20 16:40:48
       Mapping speed, Million of reads per hour |	970.51

                          Number of input reads |	7009265
                      Average input read length |	53
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4509720
                        Uniquely mapped reads % |	64.34%
                          Average mapped length |	49.75
                       Number of splices: Total |	30414
            Number of splices: Annotated (sjdb) |	11171
                       Number of splices: GT/AG |	26760
                       Number of splices: GC/AG |	1050
                       Number of splices: AT/AC |	8
               Number of splices: Non-canonical |	2596
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.24
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1115870
             % of reads mapped to multiple loci |	15.92%
        Number of reads mapped to too many loci |	86675
             % of reads mapped to too many loci |	1.24%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.88%
                     % of reads unmapped: other |	1.62%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1383675	1383675	1383675
N_multimapping	1115870	1115870	1115870
N_noFeature	923733	1051092	3242537
N_ambiguous	1740846	660315	337961
UnstrandedReadsAssigned:1845141 PositiveStrandReadsAssigned:2798313 NegativeStrandReadsAssigned:929222
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=22 echo kmer=19
SRR13079635 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079635-trimmed-pair1.fastq
                             SRR13079635-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 7,009,265 reads, 1,570,471 reads pseudoaligned
[quant] estimated average fragment length: 27.8697
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,223 rounds

 109283 SRR13079635.ke.tsv
  52636 SRR13079635.se.tsv
 161919 total
==> SRR13079635.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079635.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079635 completed mapping pipeline successfully
