Starting /dee2/code/volunteer_pipeline.sh SRR13079632
    current disk space = 414744072192
    free memory = 73717468 
SRR13079632 SRAfilesize
709d4abfd69c0d96d65973a84505067e  SRR13079632.sra
SRR13079632.sra file validated
SRR13079632 is paired end
SRR13079632 is conventional basespace
SRR13079632 read1 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079632_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87125	32.0	32.0	32.0	32.0	32.0
2	31.44175	32.0	32.0	32.0	32.0	32.0
3	31.31125	32.0	32.0	32.0	32.0	32.0
4	31.4	32.0	32.0	32.0	32.0	32.0
5	31.343	32.0	32.0	32.0	32.0	32.0
6	34.56875	36.0	36.0	36.0	32.0	36.0
7	34.3455	36.0	36.0	36.0	32.0	36.0
8	34.58375	36.0	36.0	36.0	32.0	36.0
9	34.5305	36.0	36.0	36.0	32.0	36.0
10	34.50925	36.0	36.0	36.0	32.0	36.0
11	34.43925	36.0	36.0	36.0	32.0	36.0
12	34.434	36.0	36.0	36.0	32.0	36.0
13	34.66725	36.0	36.0	36.0	32.0	36.0
14	34.5775	36.0	36.0	36.0	32.0	36.0
15	34.59525	36.0	36.0	36.0	32.0	36.0
16	34.67	36.0	36.0	36.0	32.0	36.0
17	34.83675	36.0	36.0	36.0	32.0	36.0
18	34.7065	36.0	36.0	36.0	32.0	36.0
19	34.6435	36.0	36.0	36.0	32.0	36.0
20	34.58375	36.0	36.0	36.0	32.0	36.0
21	34.685	36.0	36.0	36.0	32.0	36.0
22	34.6285	36.0	36.0	36.0	32.0	36.0
23	34.4545	36.0	36.0	36.0	32.0	36.0
24	34.69775	36.0	36.0	36.0	32.0	36.0
25	34.31625	36.0	36.0	36.0	32.0	36.0
26	34.46525	36.0	36.0	36.0	32.0	36.0
27	34.30475	36.0	36.0	36.0	32.0	36.0
28	34.352	36.0	36.0	36.0	32.0	36.0
29	34.63	36.0	36.0	36.0	32.0	36.0
30	34.3855	36.0	36.0	36.0	32.0	36.0
31	34.4685	36.0	36.0	36.0	32.0	36.0
32	34.3125	36.0	36.0	36.0	32.0	36.0
33	34.4575	36.0	36.0	36.0	32.0	36.0
34	34.34425	36.0	36.0	36.0	32.0	36.0
35	34.32	36.0	36.0	36.0	32.0	36.0
36	34.392	36.0	36.0	36.0	32.0	36.0
37	34.39875	36.0	36.0	36.0	32.0	36.0
38	34.34475	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	6.0
23	13.0
24	10.0
25	17.0
26	31.0
27	42.0
28	74.0
29	83.0
30	118.0
31	161.0
32	247.0
33	306.0
34	792.0
35	2100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.225	23.825	35.825	28.125
2	22.6	41.8	15.725	19.875
3	22.1	22.0	33.675	22.225
4	29.525000000000002	18.825	28.799999999999997	22.85
5	39.85	27.900000000000002	17.0	15.25
6	22.47247247247247	18.793793793793796	23.773773773773772	34.95995995995996
7	19.025	32.05	23.724999999999998	25.2
8	16.35	20.925	21.575	41.15
9	12.325	18.5	38.675	30.5
10	28.15	14.774999999999999	30.975	26.1
11	53.800000000000004	14.899999999999999	15.525	15.775
12	21.224999999999998	26.275	36.725	15.775
13	30.275000000000002	17.775	23.3	28.65
14	28.15	17.599999999999998	24.85	29.4
15	20.525	27.0	24.125	28.349999999999998
16	40.275	19.925	24.275	15.525
17	35.85	22.075	21.099999999999998	20.974999999999998
18	18.099999999999998	24.725	21.175	36.0
19	24.0	24.8	18.675	32.525
20	26.3	14.575	45.324999999999996	13.8
21	35.525	11.25	26.275	26.950000000000003
22	22.825	19.900000000000002	34.475	22.8
23	21.5	16.425	41.775	20.3
24	47.775	10.85	23.724999999999998	17.65
25	50.5	21.875	17.8	9.825000000000001
26	34.075	34.225	13.625000000000002	18.075
27	25.3	40.425	18.025	16.25
28	14.575	27.025	43.525000000000006	14.875
29	25.924999999999997	17.424999999999997	43.675000000000004	12.975
30	14.95	10.775	32.875	41.4
31	14.825	10.100000000000001	42.525	32.550000000000004
32	22.930732683170792	18.229557389347338	24.20605151287822	34.63365841460365
33	38.375	19.575	19.5	22.55
34	56.825	10.125	20.875	12.174999999999999
35	59.464866216554135	7.876969242310578	22.85571392848212	9.802450612653162
36	42.6	14.149999999999999	30.75	12.5
37	42.475	18.099999999999998	20.25	19.175
38	27.625	16.725	15.75	39.900000000000006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	1.0
17	1.5
18	2.0
19	2.0
20	1.5
21	1.0
22	1.5
23	2.0
24	2.0
25	2.0
26	2.0
27	2.0
28	8.0
29	14.0
30	20.5
31	27.0
32	27.0
33	31.0
34	35.0
35	35.0
36	44.5
37	54.0
38	72.5
39	91.0
40	91.0
41	168.0
42	245.0
43	257.5
44	270.0
45	270.0
46	340.0
47	410.0
48	410.0
49	443.5
50	477.0
51	475.5
52	474.0
53	474.0
54	518.5
55	563.0
56	563.0
57	491.0
58	419.0
59	463.0
60	507.0
61	507.0
62	405.0
63	303.0
64	171.5
65	40.0
66	40.0
67	30.5
68	21.0
69	21.0
70	17.0
71	13.0
72	10.5
73	8.0
74	8.0
75	5.5
76	3.0
77	3.0
78	2.5
79	2.0
80	2.5
81	3.0
82	3.0
83	2.0
84	1.0
85	1.0
86	0.5
87	0.0
88	4.0
89	8.0
90	8.0
91	4.0
92	0.0
93	0.0
94	0.0
95	0.0
96	2.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.025
33	0.0
34	0.0
35	0.025
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74651303820497	33.7
2	7.39842328684051	6.1
3	2.9108550636749544	3.5999999999999996
4	1.9405700424499697	3.2
5	0.9702850212249848	2.0
6	0.7277137659187386	1.7999999999999998
7	0.667070952092177	1.925
8	0.667070952092177	2.1999999999999997
9	0.1212856276531231	0.44999999999999996
>10	2.1831412977562157	16.675
>50	0.42449969678593086	12.75
>100	0.2425712553062462	15.6
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAAC	191	4.775	RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGC	184	4.6	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAA	137	3.4250000000000003	No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG	112	2.8000000000000003	RNA PCR Primer, Index 18 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGC	94	2.35	No Hit
AAAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTG	93	2.325	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA	75	1.875	No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTG	75	1.875	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA	66	1.6500000000000001	No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACT	54	1.35	RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGC	53	1.325	No Hit
AAGTGCTACTACTTTTGAGTCTTGGAATTCTCGGGTGC	45	1.125	No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGT	38	0.95	No Hit
TTTGGCAATGGTAGAACTCACACCTGGAATTCTCGGGT	38	0.95	No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGC	32	0.8	No Hit
CCTGCATGATGACGAACAAATACTGACTTGGAATTCTC	31	0.775	No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGTG	29	0.7250000000000001	No Hit
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGC	29	0.7250000000000001	No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGC	27	0.675	No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGC	25	0.625	No Hit
CATCAAAGTGGAGGCCCTCTCTTTGGAATTCTCGGGTG	23	0.575	No Hit
CATCAAAGTGGAGGCCCTCTCTTGGAATTCTCGGGTGC	21	0.525	No Hit
AAATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGC	19	0.475	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGTGGAAT	18	0.44999999999999996	No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGT	17	0.42500000000000004	No Hit
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGG	16	0.4	No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCC	15	0.375	No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTG	15	0.375	No Hit
AAATCTACCTGCCTCTGCCTTGGAATTCTCGGGTGCCA	15	0.375	No Hit
GGCAGAGGAGGGCTGTTCTTCCCTGGAATTCTCGGGTG	14	0.35000000000000003	No Hit
CTGCATGATGACGAACAAATACTGACTTGGAATTCTCG	13	0.325	No Hit
ACTCAAACTGGGGGCTCTTTTTGGAATTCTCGGGTGCC	13	0.325	No Hit
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCC	13	0.325	No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAA	12	0.3	RNA PCR Primer, Index 1 (100% over 23bp)
TCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGGG	12	0.3	No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGT	12	0.3	No Hit
CTCTTAGATCGATGTGGTGCTCCGGATGGAATTCTCGG	12	0.3	No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGC	12	0.3	No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGT	11	0.27499999999999997	No Hit
ATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTTGGAAT	11	0.27499999999999997	No Hit
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGC	11	0.27499999999999997	No Hit
AATCTACCTGCCTCTGCCTCCTGGAATTCTCGGGTGCC	11	0.27499999999999997	No Hit
TTTGTGACCTGGTCCACTAACCCTGGAATTCTCGGGTG	10	0.25	No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCC	10	0.25	No Hit
AGTAATGATGATTTCTGGTGATTCTGAGTTGGAATTCT	10	0.25	No Hit
GGCAGAGGAGGGCTGTTCTTCCTGGAATTCTCGGGTGC	10	0.25	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGTG	9	0.22499999999999998	No Hit
TATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8	0.2	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGG	8	0.2	No Hit
ACTCAAACTGGGGGCTCTTTTGATGGAATTCTCGGGTG	8	0.2	No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGC	8	0.2	No Hit
TAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGCC	8	0.2	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTGGAATTCTCGG	8	0.2	No Hit
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGC	8	0.2	No Hit
AGTGCCGCAGAGTTTGTAGTGTTGGAATTCTCGGGTGC	8	0.2	No Hit
TATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTG	8	0.2	No Hit
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCC	8	0.2	No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA	8	0.2	No Hit
TTTGGCAATGGTAGAACTCACACCGTGGAATTCTCGGG	7	0.17500000000000002	No Hit
CTCTTAGATCGATGTGGTGCTCCGGTGGAATTCTCGGG	7	0.17500000000000002	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTGGAATTCTCG	7	0.17500000000000002	No Hit
ACTCAAACTGTGTGACATTTTTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTG	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTC	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCACACTCTGGAATTCTCGG	7	0.17500000000000002	No Hit
AAATCTACCTGCCTCTGCCTCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTTGGA	7	0.17500000000000002	No Hit
AGCAGTGATGACAGATTGGATCTGACTTGGAATTCTCG	7	0.17500000000000002	No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
CATGATGACGAACAAATACTGACTACCTGAAGTGGAAT	6	0.15	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTTGGAATTCTC	6	0.15	No Hit
TATCGTCCTTCTGCCCAGCGATCTTGGAATTCTCGGGT	6	0.15	No Hit
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCC	6	0.15	No Hit
CAGGATCACAGAAAAAGTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTG	6	0.15	No Hit
ATGGCACTGGTAGAATTCACTGTGGAATTCTCGGGTGC	6	0.15	No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTGGAA	6	0.15	No Hit
ACAGACAGTTAGGCCTCACCTAGGCTTTTTGGAATTCT	6	0.15	No Hit
AAGTGCCGCCAGGTTTTGAGTGTTGGAATTCTCGGGTG	6	0.15	No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCA	6	0.15	No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGT	6	0.15	No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCC	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGTAAATGGAATTCTCGGG	5	0.125	No Hit
CAAGACAGATGTTCTGATCAAGGCCCGAGATTTTGGAA	5	0.125	No Hit
TTTGGCAATGGTAGAACTCACTGGAATTCTCGGGTGCC	5	0.125	No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGT	5	0.125	No Hit
TTGTGGCAACAATGGCACGGCAAGGGCGCCTTTTTGGA	5	0.125	No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGC	5	0.125	No Hit
GGCAGAGGAGGGCTGTTCTTCTGGAATTCTCGGGTGCC	5	0.125	No Hit
AACATTCAACCTGTCGGTGAGTTGGAATTCTCGGGTGC	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCC	5	0.125	No Hit
GCATGATGACGAACAAATACTGACTTGGAATTCTCGGG	5	0.125	No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTG	5	0.125	No Hit
AAAGTGCTACTACTTTTGAGTCTGGAATTCTCGGGTGC	5	0.125	No Hit
TGTAATGATGTTGATCAAATGTCTGAGCTGGAATTCTC	5	0.125	No Hit
ATAGTAGACCGTATAGCGTACGTGGAATTCTCGGGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	3.05	0.0	0.0	0.0
2	0.0	3.05	0.0	0.0	0.0
3	0.0	3.075	0.0	0.0	0.0
4	0.0	3.075	0.0	0.0	0.0
5	0.0	3.1	0.0	0.0	0.0
6	0.0	3.175	0.0	0.0	0.0
7	0.0	3.3	0.0	0.0	0.0
8	0.0	3.375	0.0	0.0	0.0
9	0.0	3.4	0.0	0.0	0.0
10	0.0	3.55	0.0	0.0	0.0
11	0.0	3.775	0.0	0.0	0.0
12	0.0	4.3	0.0	0.0	0.0
13	0.0	4.725	0.0	0.0	0.0
14	0.0	6.375	0.0	0.0	0.0
15	0.0	11.775	0.0	0.0	0.0
16	0.0	12.575	0.0	0.0	0.0
17	0.0	12.925	0.0	0.0	0.0
18	0.0	13.6	0.0	0.0	0.0
19	0.0	14.35	0.0	0.0	0.0
20	0.0	14.775	0.0	0.0	0.0
21	0.0	16.775	0.0	0.0	0.0
22	0.0	22.5	0.0	0.0	0.0
23	0.0	43.55	0.0	0.0	0.0
24	0.0	55.625	0.0	0.0	0.0
25	0.0	61.725	0.0	0.0	0.0
26	0.0	64.925	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACTT	40	8.843199E-8	32.0	4
TTGTCCC	35	1.2459041E-6	32.0	9
TTGCACT	40	8.843199E-8	32.0	3
GTCACGT	25	2.4147211E-4	32.0	29
CAGTCAC	25	2.4147211E-4	32.0	27
ACTTGTC	40	8.843199E-8	32.0	7
ACGTCCG	20	0.0033271667	32.0	32
TGTCCCG	35	1.2459041E-6	32.0	10
CCCGGCC	35	1.2459041E-6	32.0	13
CACGTCC	20	0.0033271667	32.0	31
CTCCAGT	25	2.4147211E-4	32.0	24
ACTCCAG	25	2.4147211E-4	32.0	23
TCCAGTC	25	2.4147211E-4	32.0	25
TATTGCA	35	1.2459041E-6	32.0	1
AACTCCA	25	2.4147211E-4	32.0	22
TCACGTC	20	0.0033271667	32.0	30
GTCCCGG	35	1.2459041E-6	32.0	11
ATTGCAC	40	8.843199E-8	32.0	2
GAACTCC	25	2.4147211E-4	32.0	21
CACTTGT	40	8.843199E-8	32.0	6
>>END_MODULE
SRR13079632 read2 length is 38 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079632_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	38
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86725	32.0	32.0	32.0	32.0	32.0
2	30.81675	32.0	32.0	32.0	32.0	32.0
3	30.8105	32.0	32.0	32.0	32.0	32.0
4	30.90875	32.0	32.0	32.0	32.0	32.0
5	30.9035	32.0	32.0	32.0	32.0	32.0
6	34.2705	36.0	36.0	36.0	32.0	36.0
7	34.1905	36.0	36.0	36.0	32.0	36.0
8	34.049	36.0	36.0	36.0	32.0	36.0
9	34.109	36.0	36.0	36.0	32.0	36.0
10	34.14675	36.0	36.0	36.0	32.0	36.0
11	34.03925	36.0	36.0	36.0	32.0	36.0
12	34.16775	36.0	36.0	36.0	32.0	36.0
13	34.0625	36.0	36.0	36.0	32.0	36.0
14	34.04575	36.0	36.0	36.0	32.0	36.0
15	34.1165	36.0	36.0	36.0	32.0	36.0
16	34.255	36.0	36.0	36.0	32.0	36.0
17	34.21825	36.0	36.0	36.0	32.0	36.0
18	33.94625	36.0	36.0	36.0	32.0	36.0
19	34.08975	36.0	36.0	36.0	32.0	36.0
20	33.85875	36.0	36.0	36.0	32.0	36.0
21	34.00825	36.0	36.0	36.0	32.0	36.0
22	33.93875	36.0	36.0	36.0	32.0	36.0
23	33.9325	36.0	36.0	36.0	32.0	36.0
24	33.654	36.0	36.0	36.0	27.0	36.0
25	34.00325	36.0	36.0	36.0	32.0	36.0
26	33.97325	36.0	36.0	36.0	32.0	36.0
27	34.131	36.0	36.0	36.0	32.0	36.0
28	33.9605	36.0	36.0	36.0	32.0	36.0
29	33.9905	36.0	36.0	36.0	32.0	36.0
30	33.93225	36.0	36.0	36.0	32.0	36.0
31	33.7625	36.0	36.0	36.0	32.0	36.0
32	33.491	36.0	36.0	36.0	21.0	36.0
33	33.8775	36.0	36.0	36.0	32.0	36.0
34	34.06975	36.0	36.0	36.0	32.0	36.0
35	33.8795	36.0	36.0	36.0	32.0	36.0
36	33.8625	36.0	36.0	36.0	32.0	36.0
37	33.833	36.0	36.0	36.0	32.0	36.0
38	32.8875	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	1.0
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	1.0
19	3.0
20	6.0
21	8.0
22	6.0
23	17.0
24	20.0
25	38.0
26	51.0
27	66.0
28	81.0
29	109.0
30	167.0
31	210.0
32	281.0
33	373.0
34	858.0
35	1698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.809904952476238	37.768884442221115	21.98599299649825	20.4352176088044
2	29.607401850462615	22.380595148787197	12.678169542385595	35.33383345836459
3	20.875	42.65	18.775	17.7
4	30.9	15.55	12.275	41.275
5	39.2	22.375	19.25	19.175
6	36.199999999999996	17.525	16.975	29.299999999999997
7	33.46673336668334	22.11105552776388	12.131065532766383	32.2911455727864
8	34.275706780085066	30.597948461346007	14.811108331248438	20.31523642732049
9	29.67225419064298	30.447835876907682	22.116587440580435	17.7633224918689
10	26.90863579474343	20.150187734668336	19.148936170212767	33.79224030037547
11	33.76720901126408	22.828535669586984	18.122653316645806	25.281602002503128
12	22.24724724724725	19.01901901901902	26.101101101101097	32.63263263263263
13	25.5005005005005	32.58258258258258	11.786786786786786	30.130130130130127
14	29.236545682102626	28.060075093867333	18.29787234042553	24.405506883604506
15	30.01251564455569	26.458072590738425	19.899874843554443	23.62953692115144
16	29.386733416770966	26.858573216520647	26.583229036295368	17.171464330413016
17	32.12318477716575	18.30245368052078	32.62393590385578	16.950425638457688
18	18.473091364205256	17.39674593241552	25.03128911138924	39.098873591989985
19	23.454317897371716	29.61201501877347	21.727158948685858	25.20650813516896
20	20.28042063094642	25.51326990485729	30.17025538307461	24.036054081121684
21	16.016016016016017	24.4994994994995	36.43643643643644	23.04804804804805
22	21.30162703379224	33.4918648310388	30.01251564455569	15.193992490613267
23	40.36054081121683	20.73109664496745	20.28042063094642	18.627941912869304
24	21.30162703379224	43.729662077597	17.847309136420524	17.121401752190238
25	19.09887359198999	26.307884856070086	33.09136420525657	21.501877346683354
26	21.051314142678347	19.32415519399249	26.057571964956196	33.566958698372964
27	46.820230345518276	12.769153730595894	20.0050075112669	20.40560841261893
28	31.096644967451176	9.914872308462694	38.38257386079118	20.605908863294943
29	30.620931397095642	13.845768652979471	24.787180771156734	30.74611917876815
30	46.04406609914872	11.892839258888333	18.803204807210815	23.25988983475213
31	47.34601902854281	21.532298447671508	14.071106659989985	17.050575863795693
32	31.4722083124687	33.45017526289434	12.043064596895343	23.034551827741613
33	24.211316975463195	21.75763645468202	17.200801201802705	36.830245368052076
34	24.361542313470206	13.620430645968954	38.758137205808715	23.25988983475213
35	35.42084168336673	15.631262525050099	27.229458917835668	21.718436873747496
36	25.262894341512272	16.57486229344016	46.069103655483225	12.093139709564346
37	27.366049073610416	31.372058087130693	25.53830746119179	15.723585378067101
38	41.58738107160741	31.74762143214822	14.797195793690534	11.86780170255383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	2.5
10	1.0
11	1.0
12	0.5
13	0.0
14	0.5
15	1.0
16	1.0
17	0.5
18	0.0
19	0.0
20	0.5
21	1.0
22	1.5
23	2.0
24	2.0
25	1.5
26	1.0
27	1.0
28	3.5
29	6.0
30	5.5
31	5.0
32	5.0
33	7.5
34	10.0
35	10.0
36	18.0
37	26.0
38	37.5
39	49.0
40	49.0
41	124.0
42	199.0
43	214.0
44	229.0
45	229.0
46	316.5
47	404.0
48	404.0
49	531.0
50	658.0
51	587.5
52	517.0
53	517.0
54	631.5
55	746.0
56	746.0
57	594.5
58	443.0
59	354.5
60	266.0
61	266.0
62	209.0
63	152.0
64	174.5
65	197.0
66	197.0
67	122.5
68	48.0
69	48.0
70	27.0
71	6.0
72	6.5
73	7.0
74	7.0
75	6.0
76	5.0
77	5.0
78	5.0
79	5.0
80	5.0
81	5.0
82	5.0
83	3.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.5
97	1.0
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.05
8	0.075
9	0.075
10	0.125
11	0.125
12	0.1
13	0.1
14	0.125
15	0.125
16	0.125
17	0.15
18	0.125
19	0.125
20	0.15
21	0.1
22	0.125
23	0.15
24	0.125
25	0.125
26	0.125
27	0.15
28	0.15
29	0.15
30	0.15
31	0.15
32	0.15
33	0.15
34	0.15
35	0.2
36	0.15
37	0.15
38	0.15
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.3598820058997	34.9
2	7.846607669616519	6.65
3	2.24188790560472	2.85
4	1.8289085545722714	3.1
5	0.7079646017699115	1.5
6	1.12094395280236	2.85
7	0.6489675516224189	1.925
8	0.2359882005899705	0.8
9	0.2949852507374631	1.125
>10	2.0648967551622417	16.275000000000002
>50	0.41297935103244837	12.5
>100	0.2359882005899705	15.525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGA	186	4.65	Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	183	4.575	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	141	3.5249999999999995	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	111	2.775	Illumina RNA PCR Primer (100% over 33bp)
TCAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAG	97	2.4250000000000003	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTA	89	2.225	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTA	74	1.8499999999999999	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	71	1.775	No Hit
AAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATC	65	1.625	No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAG	52	1.3	No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAA	52	1.3	Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAG	46	1.15	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGT	40	1.0	No Hit
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	39	0.975	No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAG	33	0.8250000000000001	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGA	28	0.7000000000000001	No Hit
GTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTA	27	0.675	No Hit
AGGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAG	27	0.675	No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAG	25	0.625	No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAG	24	0.6	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAG	20	0.5	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGT	20	0.5	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTA	19	0.475	No Hit
CCACGGGAACGGATCGTCGGACTGTAGAACTCTGAACG	19	0.475	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGAGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAG	18	0.44999999999999996	No Hit
ATACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGT	17	0.42500000000000004	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTG	16	0.4	No Hit
TACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	15	0.375	No Hit
AGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAGAA	15	0.375	No Hit
GGGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTA	15	0.375	No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTG	15	0.375	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGA	14	0.35000000000000003	No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGA	14	0.35000000000000003	No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGA	14	0.35000000000000003	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGATCGTCGGAC	13	0.325	No Hit
ACTCAGAATCACCAGAAATCATCATTACTGATCGTCGG	11	0.27499999999999997	No Hit
GGGTTAGTGGACCAGGTCACAAAGATCGTCGGACTGTA	11	0.27499999999999997	No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAG	11	0.27499999999999997	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGT	11	0.27499999999999997	No Hit
AGGAGCGAGCGACCAAAGGAACCATAACTGATGATCGT	11	0.27499999999999997	No Hit
TCCGGAGCACCACATCGATCTAAGAGATCGTCGGACTG	11	0.27499999999999997	No Hit
TCCGGAGCACCACATCGATCTAAGAGGATCGTCGGACT	11	0.27499999999999997	No Hit
CAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGA	11	0.27499999999999997	No Hit
GGAAGAACAGCCCTCCTCTGCCGATCGTCGGACTGTAG	10	0.25	No Hit
AACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTA	10	0.25	No Hit
AGGGGTTCACCGAGCAACATTCGATCGTCGGACTGTAG	10	0.25	No Hit
CACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTA	9	0.22499999999999998	No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGT	9	0.22499999999999998	No Hit
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGAT	9	0.22499999999999998	No Hit
GGAGGCAGAGGCAGGTAGATTGATCGTCGGACTGTAGA	9	0.22499999999999998	No Hit
TGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	9	0.22499999999999998	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTA	8	0.2	No Hit
CGTACGCTATACGGTCTACTAGATCGTCGGACTGTAGA	8	0.2	No Hit
AATCAGAACTTGACATTCAGCACAGAGTTTCTGTCCGC	8	0.2	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAG	8	0.2	No Hit
AAAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATC	7	0.17500000000000002	No Hit
CTCCGGAGCACCACATCGATCTAAGAGATCGTCGGACT	7	0.17500000000000002	No Hit
AAAGTCTCGCTCTCTGCCCCTCAGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
GCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTCG	7	0.17500000000000002	No Hit
TCAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTA	7	0.17500000000000002	No Hit
CAGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAG	7	0.17500000000000002	No Hit
GGAGTCGAGTGATGGTTCAAAGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
CCGGAGCACCACATCGATCTAAGAGGATCGTCGGACTG	7	0.17500000000000002	No Hit
GAGGCAGAGGCAGGTAGATTTGATCGTCGGACTGTAGA	7	0.17500000000000002	No Hit
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACT	7	0.17500000000000002	No Hit
TCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGGATC	7	0.17500000000000002	No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGA	6	0.15	No Hit
GAGTGTGACCAACATCAGAATCCCTTGATCGTCGGACT	6	0.15	No Hit
GCTGTTAGCCCTAGCCCCGCAGATCGTCGGACTGTAGA	6	0.15	No Hit
ACAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGT	6	0.15	No Hit
AGATCGCTGGGCAGAAGGACGATAGATCGTCGGACTGT	6	0.15	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTG	6	0.15	No Hit
CTTCAGGTAGTCAGTATTTGTTCGTCATCATGGATCGT	6	0.15	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGA	6	0.15	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAG	6	0.15	No Hit
GACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTA	6	0.15	No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCGATCG	6	0.15	No Hit
ACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGAC	6	0.15	No Hit
CCACGGGAACGTGATCGTCGGACTGTAGAACTCTGAAC	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAA	6	0.15	No Hit
AGTCAGATCCAATCTGTCATCACTGCTGATCGTCGGAC	6	0.15	No Hit
AACTCCGGAGCACCACATCGATCTAAGAGATCGTCGGA	6	0.15	No Hit
CTTTTTCTGTGATCCTGGATCGTCGGACTGTAGAACTC	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAAGCCTAGGTGAGGCCTAACTGTCTGTGATCGTCGG	6	0.15	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAG	6	0.15	No Hit
GTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	5	0.125	No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAG	5	0.125	No Hit
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAG	5	0.125	No Hit
TCGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTA	5	0.125	No Hit
GCTCAGACATTTGATCAACATCATTACAGATCGTCGGA	5	0.125	No Hit
AAAAGGCGCCCTTGCCGTGCCATTGTTGCCACAAGATC	5	0.125	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTA	5	0.125	No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGT	5	0.125	No Hit
AAATCTCGGGCCTTGATCAGAACATCTGTCTTGGATCG	5	0.125	No Hit
TTTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTG	5	0.125	No Hit
CCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAA	5	0.125	No Hit
GACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.975	0.0	0.0
2	0.0	0.0	2.975	0.0	0.0
3	0.0	0.0	3.0	0.0	0.0
4	0.0	0.0	3.0	0.0	0.0
5	0.0	0.0	3.025	0.0	0.0
6	0.0	0.0	3.075	0.0	0.0
7	0.0	0.0	3.175	0.0	0.0
8	0.0	0.0	3.25	0.0	0.0
9	0.0	0.0	3.275	0.0	0.0
10	0.0	0.0	3.45	0.0	0.0
11	0.0	0.0	3.675	0.0	0.0
12	0.0	0.0	4.225	0.0	0.0
13	0.0	0.0	4.65	0.0	0.0
14	0.0	0.0	6.275	0.0	0.0
15	0.0	0.0	11.525	0.0	0.0
16	0.0	0.0	12.3	0.0	0.0
17	0.0	0.0	12.65	0.0	0.0
18	0.0	0.0	13.35	0.0	0.0
19	0.0	0.0	14.0	0.0	0.0
20	0.0	0.0	14.4	0.0	0.0
21	0.0	0.0	16.45	0.0	0.0
22	0.0	0.0	21.925	0.0	0.0
23	0.0	0.0	43.05	0.0	0.0
24	0.0	0.0	54.825	0.0	0.0
25	0.0	0.0	60.825	0.0	0.0
26	0.0	0.0	63.925	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGATC	25	2.4147211E-4	32.0	29
AACGTGT	25	2.4147211E-4	32.0	24
AACGTGG	20	0.0033271667	32.0	8
TGCAATA	40	8.843199E-8	32.0	16
GTGTAGA	25	2.4147211E-4	32.0	27
CCACGGG	25	2.4147211E-4	32.0	1
GGCCGGG	40	8.843199E-8	32.0	4
GGACAAG	40	8.843199E-8	32.0	9
GCAATAG	40	8.843199E-8	32.0	17
CAATAGA	40	8.843199E-8	32.0	18
CTGAACG	25	2.4147211E-4	32.0	21
AGGCCGG	40	8.843199E-8	32.0	3
CGGGACA	40	8.843199E-8	32.0	7
CGGGAAC	25	2.4147211E-4	32.0	4
ACGGGAA	25	2.4147211E-4	32.0	3
TGTAGAT	25	2.4147211E-4	32.0	28
GGGACAA	40	8.843199E-8	32.0	8
CGTGTAG	25	2.4147211E-4	32.0	26
CCGGGAC	40	8.843199E-8	32.0	6
CAGGCCG	40	8.843199E-8	32.0	2
>>END_MODULE
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696147 spots for SRR13079632.sra
Written 696147 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
Read 696143 spots for SRR13079632.sra
Written 696143 spots for SRR13079632.sra
SRR ids: ['SRR13079632.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8so00202
SRR13079632.sra spots: 8353720
blocks: [[1, 696143], [696144, 1392286], [1392287, 2088429], [2088430, 2784572], [2784573, 3480715], [3480716, 4176858], [4176859, 4873001], [4873002, 5569144], [5569145, 6265287], [6265288, 6961430], [6961431, 7657573], [7657574, 8353720]]
SRR13079632 file size 968624
SRR13079632 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 12 -o SRR13079632 SRR13079632_1.fastq SRR13079632_2.fastq
Input file:	SRR13079632_1.fastq
Paired file:	SRR13079632_2.fastq
trimmed:	SRR13079632-trimmed-pair1.fastq, SRR13079632-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:34:39 2021 >> started

Tue Apr 20 16:34:47 2021 >> done (8.327s)
8353720 read pairs processed; of these:
   4262 ( 0.05%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
8349458 (99.95%) read pairs available; of these:
   4871 ( 0.06%) trimmed read pairs available after processing
8344587 (99.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 27	      4	  0.00%
 28	    148	  0.00%
 29	    606	  0.01%
 30	    189	  0.00%
 31	    468	  0.01%
 32	    478	  0.01%
 33	    167	  0.00%
 34	    349	  0.00%
 35	    112	  0.00%
 36	    724	  0.01%
 37	   1626	  0.02%
 38	8344587	 99.94%
8349458 reads passed initial QC


criterion=sequence-density
sequence-density=67.80
sequence-density-rank=1
fanout-score=16.94
fanout-score-rank=1
prefix-density=70.95
prefix-fanout=16.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC


criterion=fanout-score
sequence-density=67.80
sequence-density-rank=1
fanout-score=16.94
fanout-score-rank=1
prefix-density=70.95
prefix-fanout=16.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC


criterion=sequence-density
sequence-density=65.86
sequence-density-rank=1
fanout-score=17.28
fanout-score-rank=1
prefix-density=69.02
prefix-fanout=16.5
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG


criterion=fanout-score
sequence-density=65.86
sequence-density-rank=1
fanout-score=17.28
fanout-score-rank=1
prefix-density=69.02
prefix-fanout=16.5
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 12 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC -y GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG -o SRR13079632 SRR13079632_1.fastq SRR13079632_2.fastq
Input file:	SRR13079632_1.fastq
Paired file:	SRR13079632_2.fastq
trimmed:	SRR13079632-trimmed-pair1.fastq, SRR13079632-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC
-- paired 3' end adapter sequence (-y):	GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	12
Tue Apr 20 16:35:39 2021 >> started

Tue Apr 20 16:35:46 2021 >> done (6.910s)
8100221 read pairs processed; of these:
1183542 (14.61%) short read pairs filtered out after trimming by size control
 301282 ( 3.72%) empty read pairs filtered out after trimming by size control
6615397 (81.67%) read pairs available; of these:
6055819 (91.54%) trimmed read pairs available after processing
 559578 ( 8.46%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  84034	  1.27%
 19	  60783	  0.92%
 20	 157893	  2.39%
 21	 453545	  6.86%
 22	1626116	 24.58%
 23	 887502	 13.42%
 24	 500067	  7.56%
 25	 257285	  3.89%
 26	 165390	  2.50%
 27	 154118	  2.33%
 28	 184699	  2.79%
 29	 131651	  1.99%
 30	 116502	  1.76%
 31	 106369	  1.61%
 32	 175253	  2.65%
 33	 425217	  6.43%
 34	 447901	  6.77%
 35	  73422	  1.11%
 36	  30899	  0.47%
 37	  18524	  0.28%
 38	 558227	  8.44%


criterion=sequence-density
sequence-density=11.67
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=9
prefix-density=0.00
prefix-fanout=1.0
sequence=TATTGCACTTGTCCCGGCCTGTAAA


criterion=fanout-score
sequence-density=0.50
sequence-density-rank=19
fanout-score=32.61
fanout-score-rank=1
prefix-density=13.14
prefix-fanout=1.2
sequence=GTCCCGGCCTGA


criterion=sequence-density
sequence-density=2.61
sequence-density-rank=1
fanout-score=4.01
fanout-score-rank=3
prefix-density=0.09
prefix-fanout=4.0
sequence=AGCCAAGCTCAGACGGATCCGA


criterion=fanout-score
sequence-density=0.42
sequence-density-rank=9
fanout-score=15.34
fanout-score-rank=1
prefix-density=0.44
prefix-fanout=14.6
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAG
SRR13079632 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:36:40
                             Started mapping on |	Apr 20 16:36:41
                                    Finished on |	Apr 20 16:37:22
       Mapping speed, Million of reads per hour |	602.75

                          Number of input reads |	6864634
                      Average input read length |	53
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4284164
                        Uniquely mapped reads % |	62.41%
                          Average mapped length |	47.21
                       Number of splices: Total |	12540
            Number of splices: Annotated (sjdb) |	5090
                       Number of splices: GT/AG |	10330
                       Number of splices: GC/AG |	583
                       Number of splices: AT/AC |	24
               Number of splices: Non-canonical |	1603
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.22
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.01
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	530384
             % of reads mapped to multiple loci |	7.73%
        Number of reads mapped to too many loci |	56973
             % of reads mapped to too many loci |	0.83%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	24.35%
                     % of reads unmapped: other |	4.68%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2050086	2050086	2050086
N_multimapping	530384	530384	530384
N_noFeature	487754	565376	3114518
N_ambiguous	2091019	1129970	165432
UnstrandedReadsAssigned:1705391 PositiveStrandReadsAssigned:2588818 NegativeStrandReadsAssigned:1004214
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=22 echo kmer=19
SRR13079632 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079632-trimmed-pair1.fastq
                             SRR13079632-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 6,864,634 reads, 1,705,469 reads pseudoaligned
[quant] estimated average fragment length: 27.7492
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,096 rounds

 109283 SRR13079632.ke.tsv
  52636 SRR13079632.se.tsv
 161919 total
==> SRR13079632.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079632.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079632 completed mapping pipeline successfully
