Starting /dee2/code/volunteer_pipeline.sh SRR13079638
    current disk space = 7072838936
    free memory = 131617256 
SRR13079638 SRAfilesize
6c30a4e963a68d79e3cbf6d3823ae3f8  SRR13079638.sra
SRR13079638.sra file validated
SRR13079638 is paired end
SRR13079638 is conventional basespace
SRR13079638 read1 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.009	32.0	32.0	32.0	32.0	32.0
2	31.45375	32.0	32.0	32.0	32.0	32.0
3	31.4715	32.0	32.0	32.0	32.0	32.0
4	31.48775	32.0	32.0	32.0	32.0	32.0
5	31.41425	32.0	32.0	32.0	32.0	32.0
6	35.07525	36.0	36.0	36.0	36.0	36.0
7	35.09725	36.0	36.0	36.0	36.0	36.0
8	35.0095	36.0	36.0	36.0	36.0	36.0
9	34.87575	36.0	36.0	36.0	36.0	36.0
10	34.90575	36.0	36.0	36.0	36.0	36.0
11	35.01475	36.0	36.0	36.0	36.0	36.0
12	34.9645	36.0	36.0	36.0	36.0	36.0
13	35.059	36.0	36.0	36.0	36.0	36.0
14	35.13425	36.0	36.0	36.0	36.0	36.0
15	35.014	36.0	36.0	36.0	36.0	36.0
16	34.95575	36.0	36.0	36.0	36.0	36.0
17	35.001	36.0	36.0	36.0	36.0	36.0
18	35.09225	36.0	36.0	36.0	36.0	36.0
19	35.142	36.0	36.0	36.0	36.0	36.0
20	35.0165	36.0	36.0	36.0	36.0	36.0
21	35.16975	36.0	36.0	36.0	36.0	36.0
22	35.09525	36.0	36.0	36.0	36.0	36.0
23	34.9925	36.0	36.0	36.0	32.0	36.0
24	35.0295	36.0	36.0	36.0	36.0	36.0
25	34.81175	36.0	36.0	36.0	36.0	36.0
26	34.96775	36.0	36.0	36.0	36.0	36.0
27	34.831	36.0	36.0	36.0	32.0	36.0
28	34.9955	36.0	36.0	36.0	36.0	36.0
29	34.9325	36.0	36.0	36.0	36.0	36.0
30	35.03025	36.0	36.0	36.0	36.0	36.0
31	34.8115	36.0	36.0	36.0	32.0	36.0
32	34.8615	36.0	36.0	36.0	32.0	36.0
33	34.96775	36.0	36.0	36.0	36.0	36.0
34	34.921	36.0	36.0	36.0	36.0	36.0
35	34.856	36.0	36.0	36.0	32.0	36.0
36	34.7565	36.0	36.0	36.0	32.0	36.0
37	34.666	36.0	36.0	36.0	32.0	36.0
38	34.73475	36.0	36.0	36.0	32.0	36.0
39	34.60875	36.0	36.0	36.0	32.0	36.0
40	34.85875	36.0	36.0	36.0	36.0	36.0
41	34.59575	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	3.0
24	3.0
25	15.0
26	14.0
27	27.0
28	52.0
29	73.0
30	101.0
31	112.0
32	142.0
33	249.0
34	622.0
35	2584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.625	40.025	26.400000000000002	20.95
2	28.4	27.525	20.7	23.375
3	21.425	20.625	34.775	23.175
4	36.275	16.8	21.875	25.05
5	26.75	31.374999999999996	19.55	22.325
6	28.103103103103106	27.102102102102105	19.01901901901902	25.775775775775777
7	28.575	31.05	20.849999999999998	19.525000000000002
8	19.675	20.599999999999998	23.275000000000002	36.449999999999996
9	20.424999999999997	24.224999999999998	38.550000000000004	16.8
10	39.975	19.45	22.8	17.775
11	39.85	22.6	19.825	17.724999999999998
12	33.025	24.825	24.6	17.549999999999997
13	28.1	21.099999999999998	34.1	16.7
14	38.1	21.825	26.200000000000003	13.875000000000002
15	20.875	22.6	32.725	23.799999999999997
16	27.55	24.25	33.5	14.7
17	25.474999999999998	18.925	29.425	26.174999999999997
18	21.15	28.975	33.2	16.675
19	32.300000000000004	22.6	29.349999999999998	15.75
20	23.5	22.05	38.25	16.2
21	31.624999999999996	17.575	31.225	19.575
22	25.85	30.9	27.1	16.150000000000002
23	27.425	43.125	12.475	16.975
24	39.175	36.75	12.925	11.15
25	24.975	40.475	18.6	15.950000000000001
26	18.475	22.25	30.625000000000004	28.65
27	25.474999999999998	17.75	17.75	39.025
28	45.025	15.0	14.524999999999999	25.45
29	43.475	27.474999999999998	12.075	16.975
30	31.65	44.65	7.8	15.9
31	33.675	45.225	9.65	11.450000000000001
32	37.9	40.975	7.55	13.575000000000001
33	30.2	45.75	7.8	16.25
34	38.725	29.675	7.5249999999999995	24.075
35	23.075000000000003	32.300000000000004	7.8	36.825
36	18.525	44.275	7.5249999999999995	29.675
37	18.025	35.775	7.449999999999999	38.75
38	21.099999999999998	38.9	9.225	30.775000000000002
39	28.925	23.9	9.325	37.85
40	38.4	18.525	20.65	22.425
41	19.3	17.299999999999997	35.8	27.6
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	1.5
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	1.0
16	0.5
17	0.0
18	0.5
19	1.0
20	1.0
21	1.0
22	1.0
23	2.0
24	3.0
25	3.0
26	2.0
27	1.0
28	2.5
29	4.0
30	7.0
31	10.0
32	10.0
33	13.5
34	17.0
35	24.5
36	32.0
37	32.0
38	53.5
39	75.0
40	114.5
41	154.0
42	154.0
43	213.5
44	273.0
45	404.0
46	535.0
47	535.0
48	522.5
49	510.0
50	718.5
51	927.0
52	808.5
53	690.0
54	690.0
55	495.0
56	300.0
57	267.0
58	234.0
59	234.0
60	182.5
61	131.0
62	99.5
63	68.0
64	68.0
65	39.5
66	11.0
67	10.0
68	9.0
69	9.0
70	6.5
71	4.0
72	2.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.35025617140197	45.275
2	7.03306939916162	7.55
3	2.5151374010246856	4.05
4	1.6301816488122962	3.5000000000000004
5	1.0246856078248718	2.75
6	0.4657661853749418	1.5
7	0.4657661853749418	1.7500000000000002
8	0.3260363297624593	1.4000000000000001
9	0.18630647414997673	0.8999999999999999
>10	1.7233348858872848	17.25
>50	0.18630647414997673	7.1
>100	0.09315323707498836	6.9750000000000005
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACTCAAACTGGGGGCTCTTTTAGATCGGAAGAGCACACGTC	155	3.875	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGT	124	3.1	No Hit
AGTGCCGCAGAGTTTGTAGTGTAGATCGGAAGAGCACACGT	97	2.4250000000000003	No Hit
ACTCAAACTGGGGGCTCTTTTGAGATCGGAAGAGCACACGT	71	1.775	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA	58	1.4500000000000002	TruSeq Adapter, Index 7 (97% over 36bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTT	58	1.4500000000000002	No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGT	47	1.175	No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACAC	44	1.0999999999999999	No Hit
AGTGCCGCAGAGTTTGTAGTGAGATCGGAAGAGCACACGTC	40	1.0	No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTT	35	0.8750000000000001	No Hit
AAAGTGCTTCCCTTTTGTGTGTAGATCGGAAGAGCACACGT	34	0.8500000000000001	No Hit
TATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGT	29	0.7250000000000001	No Hit
ACTCAAACTGTGTGACATTTTAGATCGGAAGAGCACACGTC	29	0.7250000000000001	No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGT	24	0.6	No Hit
TTTGGCAATGGTAGAACTCACACCAGATCGGAAGAGCACAC	23	0.575	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGT	22	0.5499999999999999	No Hit
AAGGGATTCTGATGTTGGTCACACTAGATCGGAAGAGCACA	20	0.5	No Hit
AGTGCCGCAGAGTTTGTAGTGCAGATCGGAAGAGCACACGT	18	0.44999999999999996	No Hit
ACTCAAACTGGGGGCTCTTTCAGATCGGAAGAGCACACGTC	18	0.44999999999999996	No Hit
AAAGTGCCGCCAGGTTTTGAGTGTAGATCGGAAGAGCACAC	18	0.44999999999999996	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGG	18	0.44999999999999996	No Hit
CATCAAAGTGGAGGCCCTCTCTAGATCGGAAGAGCACACGT	16	0.4	No Hit
AAGTGCCGCCAGGTTTTGAGTGTAGATCGGAAGAGCACACG	16	0.4	No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGT	16	0.4	No Hit
TGGTAGACTATGGAACGTAGGAGATCGGAAGAGCACACGTC	15	0.375	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAA	14	0.35000000000000003	No Hit
CTCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCA	14	0.35000000000000003	No Hit
AAAGTGCTTCCACTTTGTGTGCAGATCGGAAGAGCACACGT	13	0.325	No Hit
AAACATTCGCGGTGCACTTCTTAGATCGGAAGAGCACACGT	13	0.325	No Hit
TTTGGCAATGGTAGAACTCACACCGAGATCGGAAGAGCACA	13	0.325	No Hit
CACCCGTAGAACCGACCTTGCGAGATCGGAAGAGCACACGT	12	0.3	No Hit
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGT	12	0.3	No Hit
TATGGCACTGGTAGAATTCACTGAGATCGGAAGAGCACACG	12	0.3	No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACG	12	0.3	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	11	0.27499999999999997	No Hit
ATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAGGGCCTA	11	0.27499999999999997	No Hit
ATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGTC	11	0.27499999999999997	No Hit
AAGGGATTCTGATGTTGGTCACACTCAGATCGGAAGAGCAC	10	0.25	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGAGATCGGAA	10	0.25	No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGT	10	0.25	No Hit
ATGGCACTGGTAGAATTCACTGAGATCGGAAGAGCACACGT	10	0.25	No Hit
AAAGTGCTACTACTTTTGAGTCTAGATCGGAAGAGCACACG	10	0.25	No Hit
CAAAGTGCTGTTCGTGCAGGTAGAGATCGGAAGAGCACACG	10	0.25	No Hit
CCTGCATGATGACGAACAAATACTGACTAGATCGGAAGAGC	9	0.22499999999999998	No Hit
AAGGGATTCTGATGTTGGTCACAAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
TCTTAGATCGATGTGGTGCTCCGGAGTTCTCTTCGGGCCAG	9	0.22499999999999998	No Hit
CAACGGAATCCCAAAAGCAGCTGAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACAC	8	0.2	No Hit
GCCTGCTGGGGTGGAACCTGGTAGATCGGAAGAGCACACGT	8	0.2	No Hit
ACTCAAACTGGGGGCTCTTTAGATCGGAAGAGCACACGTCT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATCAAAGTGGAGGCCCTCTCTTAGATCGGAAGAGCACACG	8	0.2	No Hit
ATCATGATGGGCTCCTCGGTGTAGATCGGAAGAGCACACGT	8	0.2	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGAT	8	0.2	No Hit
AAAGTGCTACTACTTTTGAGTCCAGATCGGAAGAGCACACG	8	0.2	No Hit
GAATGTTGCTCGGTGAACCCCTAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTAGATCGGA	7	0.17500000000000002	No Hit
ACTCAAACTATGGGGGCACTTTAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
AAGGGATTCTGATGTTGGTCAGATCGGAAGAGCACACGTCT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
ACTCAAACTGGGGGCTCTTTTGTAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTG	7	0.17500000000000002	No Hit
AGTGCCGCAGAGTTTGTAGTAAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
ACTCAAACTGTGTGACATTTAGATCGGAAGAGCACACGTCT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTCTTTCCGTGTGGATTTAAGAATTTTTAGATCGGAAG	7	0.17500000000000002	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAG	6	0.15	No Hit
TGGAAGACTAGTGATTTTGTTGTAGATCGGAAGAGCACACG	6	0.15	No Hit
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACG	6	0.15	No Hit
AGTGCCGCAGAGTTTGTAGTGTTAGATCGGAAGAGCACACG	6	0.15	No Hit
TGGAAGACTAGTGATTTTGTTGTCAGATCGGAAGAGCACAC	6	0.15	No Hit
AAGTGCTACTACTTTTGAGTCTAGATCGGAAGAGCACACGT	6	0.15	No Hit
AAAGTGCTTCCCTTTTGTGTGCAGATCGGAAGAGCACACGT	6	0.15	No Hit
TAAAGTGCTTATAGTGCAGGTAGAGATCGGAAGAGCACACG	6	0.15	No Hit
AGTGCCGCAGAGTTTGTAGTGGAGATCGGAAGAGCACACGT	6	0.15	No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAAGTGCTTACAGTGCAGGTAGAGATCGGAAGAGCACACG	5	0.125	No Hit
TCTTAGATCGATGTGGTGCTAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCCGTAGAACCGACCTTGCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGAAGATCGGAAGAGCACACGT	5	0.125	No Hit
AGCTCGGTCTGAGGCCCCTCAGTAGATCGGAAGAGCACACG	5	0.125	No Hit
TTTTTCATTATTGCTCCTGACCAGATCGGAAGAGCACACGT	5	0.125	No Hit
AAACATTCGCGGTGCACTTCTCAGATCGGAAGAGCACACGT	5	0.125	No Hit
TATGGCACTGGTAGAATTCACCAGATCGGAAGAGCACACGT	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGCAGATCGGAAGAGCACACGT	5	0.125	No Hit
TGTAAACATCCTACACTCTCAGCTAGATCGGAAGAGCACAC	5	0.125	No Hit
TGAGGTTGGTGTACTGTGTGTGAAGATCGGAAGAGCACACG	5	0.125	No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG	5	0.125	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG	5	0.125	No Hit
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGT	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATC	5	0.125	No Hit
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACG	5	0.125	No Hit
TCAGTGCACTACAGAACTTTGGAGATCGGAAGAGCACACGT	5	0.125	No Hit
AGCAGGCTCACTCTTTAGATCGGAAGAGCACACGTCTGAAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTAGATCGG	5	0.125	No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.575	0.0	0.0	0.0	0.0
2	1.625	0.0	0.0	0.0	0.0
3	1.625	0.0	0.0	0.0	0.0
4	1.625	0.0	0.0	0.0	0.0
5	1.65	0.0	0.0	0.0	0.0
6	1.65	0.0	0.0	0.0	0.0
7	1.65	0.0	0.0	0.0	0.0
8	1.7	0.0	0.0	0.0	0.0
9	1.95	0.0	0.0	0.0	0.0
10	2.75	0.0	0.0	0.0	0.0
11	3.325	0.0	0.0	0.0	0.0
12	4.1	0.0	0.0	0.0	0.0
13	5.35	0.0	0.0	0.0	0.0
14	6.6	0.0	0.0	0.0	0.0
15	7.75	0.0	0.0	0.0	0.0
16	9.1	0.0	0.0	0.0	0.0
17	10.35	0.0	0.0	0.0	0.0
18	11.425	0.0	0.0	0.0	0.0
19	12.675	0.0	0.0	0.0	0.0
20	14.15	0.0	0.0	0.0	0.0
21	17.2	0.0	0.0	0.0	0.0
22	29.125	0.0	0.0	0.0	0.0
23	50.925	0.0	0.0	0.0	0.0
24	59.7	0.0	0.0	0.0	0.0
25	65.5	0.0	0.0	0.0	0.0
26	67.825	0.0	0.0	0.0	0.0
27	69.075	0.0	0.0	0.0	0.0
28	70.225	0.0	0.0	0.0	0.0
29	72.225	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTGG	45	2.588422E-9	35.000004	5
AACTGGG	45	2.588422E-9	35.000004	6
CTGGGGG	45	2.588422E-9	35.000004	8
CTCTTTT	35	6.175269E-7	35.000004	15
GGGGGCT	45	2.588422E-9	35.000004	10
GGCTCTT	45	2.588422E-9	35.000004	13
GGGGCTC	45	2.588422E-9	35.000004	11
GCTCTTT	45	2.588422E-9	35.000004	14
ACTGGGG	45	2.588422E-9	35.000004	7
CAAACTG	45	2.588422E-9	35.000004	4
GGGCTCT	45	2.588422E-9	35.000004	12
GTTTGTA	20	0.0021434545	35.0	12
TTGTAGT	20	0.0021434545	35.0	14
CCGCAGA	20	0.0021434545	35.0	5
GAGTTTG	20	0.0021434545	35.0	10
CAGAGTT	20	0.0021434545	35.0	8
ACTCAAA	50	1.6552804E-10	35.0	1
AGAGTTT	20	0.0021434545	35.0	9
TCAAACT	50	1.6552804E-10	35.0	3
ATTGCAC	20	0.0021434545	35.0	2
>>END_MODULE
SRR13079638 read2 length is 41 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR13079638_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	41
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991	32.0	32.0	32.0	32.0	32.0
2	30.9195	32.0	32.0	32.0	32.0	32.0
3	30.7725	32.0	32.0	32.0	32.0	32.0
4	30.74325	32.0	32.0	32.0	32.0	32.0
5	30.64575	32.0	32.0	32.0	32.0	32.0
6	34.14625	36.0	36.0	36.0	32.0	36.0
7	34.267	36.0	36.0	36.0	32.0	36.0
8	34.1985	36.0	36.0	36.0	32.0	36.0
9	34.2695	36.0	36.0	36.0	32.0	36.0
10	34.10925	36.0	36.0	36.0	32.0	36.0
11	34.241	36.0	36.0	36.0	32.0	36.0
12	34.05325	36.0	36.0	36.0	32.0	36.0
13	34.22625	36.0	36.0	36.0	32.0	36.0
14	34.22425	36.0	36.0	36.0	32.0	36.0
15	34.2285	36.0	36.0	36.0	32.0	36.0
16	34.40575	36.0	36.0	36.0	32.0	36.0
17	34.44075	36.0	36.0	36.0	32.0	36.0
18	34.364	36.0	36.0	36.0	32.0	36.0
19	34.28475	36.0	36.0	36.0	32.0	36.0
20	34.13375	36.0	36.0	36.0	32.0	36.0
21	34.4095	36.0	36.0	36.0	32.0	36.0
22	34.143	36.0	36.0	36.0	32.0	36.0
23	34.45825	36.0	36.0	36.0	32.0	36.0
24	34.15475	36.0	36.0	36.0	32.0	36.0
25	34.2325	36.0	36.0	36.0	32.0	36.0
26	34.18825	36.0	36.0	36.0	32.0	36.0
27	34.0615	36.0	36.0	36.0	32.0	36.0
28	34.06075	36.0	36.0	36.0	32.0	36.0
29	34.109	36.0	36.0	36.0	32.0	36.0
30	34.178	36.0	36.0	36.0	32.0	36.0
31	34.27075	36.0	36.0	36.0	32.0	36.0
32	34.29825	36.0	36.0	36.0	32.0	36.0
33	34.30075	36.0	36.0	36.0	32.0	36.0
34	34.2235	36.0	36.0	36.0	32.0	36.0
35	34.1705	36.0	36.0	36.0	32.0	36.0
36	34.08525	36.0	36.0	36.0	32.0	36.0
37	33.9555	36.0	36.0	36.0	32.0	36.0
38	33.95	36.0	36.0	36.0	32.0	36.0
39	34.0955	36.0	36.0	36.0	32.0	36.0
40	33.68525	36.0	36.0	36.0	32.0	36.0
41	33.21	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	2.0
17	4.0
18	6.0
19	6.0
20	2.0
21	10.0
22	17.0
23	15.0
24	25.0
25	35.0
26	56.0
27	57.0
28	91.0
29	119.0
30	114.0
31	145.0
32	199.0
33	277.0
34	777.0
35	2039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.975	45.525	15.075	19.425
2	23.974999999999998	35.449999999999996	8.475000000000001	32.1
3	13.925	42.25	20.95	22.875
4	19.375	35.475	13.625000000000002	31.525
5	23.849999999999998	37.574999999999996	21.375	17.2
6	26.900000000000002	27.425	15.875	29.799999999999997
7	20.225	30.8	20.025000000000002	28.95
8	21.2	30.425	16.875	31.5
9	18.575	28.125	21.275	32.025
10	14.174999999999999	26.900000000000002	26.35	32.574999999999996
11	24.7	21.525	18.8	34.975
12	17.925	19.25	28.075	34.75
13	24.474999999999998	31.8	16.650000000000002	27.075
14	26.474999999999998	23.724999999999998	26.625	23.175
15	24.825	20.674999999999997	26.125	28.375
16	28.4	22.3	26.674999999999997	22.625
17	27.425	23.275000000000002	28.749999999999996	20.549999999999997
18	31.85	20.349999999999998	24.95	22.85
19	25.724999999999998	25.674999999999997	17.8	30.8
20	28.65	25.974999999999998	25.35	20.025000000000002
21	19.825	18.575	31.924999999999997	29.675
22	24.75	22.05	34.949999999999996	18.25
23	41.575	24.6	18.075	15.75
24	20.9	43.575	22.05	13.475000000000001
25	18.625	20.325	32.550000000000004	28.499999999999996
26	24.075	16.75	25.674999999999997	33.5
27	35.05	14.774999999999999	30.95	19.225
28	24.15	10.85	39.45	25.55
29	33.275	11.325000000000001	18.175	37.225
30	46.275	19.35	16.625	17.75
31	41.325	28.65	12.9	17.125
32	26.5	31.65	18.6	23.25
33	20.075000000000003	27.224999999999998	21.05	31.65
34	29.675	16.35	35.975	18.0
35	32.475	19.175	29.875	18.475
36	22.3	22.6	42.875	12.225
37	28.4	33.975	19.975	17.65
38	42.225	32.05	15.4	10.325
39	22.900000000000002	47.325	10.975	18.8
40	17.25	47.875	14.6	20.275000000000002
41	22.45	24.224999999999998	21.175	32.15
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.0
7	1.0
8	1.0
9	2.0
10	3.0
11	3.0
12	3.0
13	4.0
14	5.0
15	5.0
16	3.0
17	1.0
18	2.0
19	3.0
20	3.0
21	1.5
22	0.0
23	1.5
24	3.0
25	3.0
26	3.0
27	3.0
28	3.5
29	4.0
30	7.0
31	10.0
32	10.0
33	9.5
34	9.0
35	20.0
36	31.0
37	31.0
38	39.0
39	47.0
40	122.5
41	198.0
42	198.0
43	245.0
44	292.0
45	420.0
46	548.0
47	548.0
48	714.5
49	881.0
50	856.5
51	832.0
52	630.0
53	428.0
54	428.0
55	322.5
56	217.0
57	194.0
58	171.0
59	171.0
60	153.5
61	136.0
62	118.5
63	101.0
64	101.0
65	63.5
66	26.0
67	24.0
68	22.0
69	22.0
70	15.5
71	9.0
72	6.0
73	3.0
74	2.0
75	1.0
76	1.0
77	1.0
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	1.0
85	2.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
41	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.974999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.34444931987714	48.625
2	6.976744186046512	7.95
3	2.369460289600702	4.05
4	1.5357612988152698	3.5000000000000004
5	0.7898200965335673	2.25
6	0.5704256252742431	1.95
7	0.4387889425186486	1.7500000000000002
8	0.26327336551118913	1.2
9	0.08775778850372971	0.44999999999999996
>10	1.4041246160596754	15.375
>50	0.13163668275559456	5.45
>100	0.08775778850372971	7.449999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	154	3.85	No Hit
AAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	144	3.5999999999999996	No Hit
ACACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAAC	93	2.325	No Hit
CAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAAC	65	1.625	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	60	1.5	Illumina RNA PCR Primer (100% over 36bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	47	1.175	No Hit
GCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	44	1.0999999999999999	No Hit
AACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGA	41	1.0250000000000001	No Hit
CACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAACT	35	0.8750000000000001	No Hit
ACACACAAAAGGGAAGCACTTTGATCGTCGGACTGTAGAAC	32	0.8	No Hit
AGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAAC	30	0.75	No Hit
AAAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGAACT	27	0.675	No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAAC	23	0.575	No Hit
GGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	22	0.5499999999999999	No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAAC	20	0.5	No Hit
ACACTCAAAACCTGGCGGCACTTTGATCGTCGGACTGTAGA	18	0.44999999999999996	No Hit
AGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTAG	18	0.44999999999999996	No Hit
GAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACT	17	0.42500000000000004	No Hit
ACACTCAAAACCTGGCGGCACTTGATCGTCGGACTGTAGAA	16	0.4	No Hit
TCAGACCGAGACAAGTGCAATGGATCGTCGGACTGTAGAAC	16	0.4	No Hit
CCTACGTTCCATAGTCTACCAGATCGTCGGACTGTAGAACT	15	0.375	No Hit
TTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCGTC	15	0.375	No Hit
AGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAGAAC	15	0.375	No Hit
GCACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAAC	15	0.375	No Hit
CGGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAG	13	0.325	No Hit
CAGTGAATTCTACCAGTGCCATAGATCGTCGGACTGTAGAA	13	0.325	No Hit
GTCAACATCAGTCTGATAAGCTAGATCGTCGGACTGTAGAA	13	0.325	No Hit
CGCACGAGCCGAGTGATCCACCGCTAAGAGTCGATCGTCGG	13	0.325	No Hit
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAAC	12	0.3	No Hit
GCACACAAAGTGGAAGCACTTTGATCGTCGGACTGTAGAAC	12	0.3	No Hit
TACAGATGGATACCGTGCAATTGATCGTCGGACTGTAGAAC	12	0.3	No Hit
AGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAGAACT	11	0.27499999999999997	No Hit
CAGTGAATTCTACCAGTGCCATGATCGTCGGACTGTAGAAC	10	0.25	No Hit
AAGAAGTGCACCGCGAATGTTTGATCGTCGGACTGTAGAAC	10	0.25	No Hit
AGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTAGAA	10	0.25	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTGATCGTCGG	10	0.25	No Hit
GGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAAC	10	0.25	No Hit
CTACCTGCACGAACAGCACTTTGGATCGTCGGACTGTAGAA	9	0.22499999999999998	No Hit
AGTCAGTATTTGTTCGTCATCATGCAGGGATCGTCGGACTG	9	0.22499999999999998	No Hit
CCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	8	0.2	No Hit
AAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAACTC	8	0.2	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGTGAGTTCTACCATTGCCAAAGATCGTCGGACTGTAGA	8	0.2	No Hit
TCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAG	8	0.2	No Hit
GAGTGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTA	8	0.2	No Hit
AAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATC	8	0.2	No Hit
ACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
TGTGACCAACATCAGAATCCCTTGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
AAAGTGCCCCCATAGTTTGAGTGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
ACCAGGTTCCACCCCAGCAGGCGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
GGACTCAAAAGTAGTAGCACTTTGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
AAATGTCACACAGTTTGAGTGATCGTCGGACTGTAGAACTC	7	0.17500000000000002	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
AAAAATTCTTAAATCCACACGGAAAGAGGGCGATCGTCGGA	7	0.17500000000000002	No Hit
ACACCGAGGAGCCCATCATGATGATCGTCGGACTGTAGAAC	7	0.17500000000000002	No Hit
AAGAGAGGGCCTCCACTTTGATGGATCGTCGGACTGTAGAA	7	0.17500000000000002	No Hit
TGCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGC	6	0.15	No Hit
ACGCACGAGCCGAGTGATCCACCGCTAAGAGTCGATCGTCG	6	0.15	No Hit
CGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCT	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGA	6	0.15	No Hit
ACAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAA	6	0.15	No Hit
TACTACAAACTCTGCGGCACTGATCGTCGGACTGTAGAACT	6	0.15	No Hit
TCACACACAGTACACCAACCTCAGATCGTCGGACTGTAGAA	6	0.15	No Hit
AGACTCAAAAGTAGTAGCACTTGATCGTCGGACTGTAGAAC	6	0.15	No Hit
AAATGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGG	6	0.15	No Hit
AGGGGTTCACCGAGCAACATTCGATCGTCGGACTGTAGAAC	6	0.15	No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAA	6	0.15	No Hit
GCACACAAAAGGGAAGCACTTTGATCGTCGGACTGTAGAAC	6	0.15	No Hit
GACCAACATCAGAATCCCTTGATCGTCGGACTGTAGAACTC	6	0.15	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAGAGTGAGCCTGCTGATCGTCGGACTGTAGAACTCTGAA	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAAGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCC	5	0.125	No Hit
GGTCAGGAGCAATAATGAAAAAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGA	5	0.125	No Hit
ACGCACGAGCCGAGTGATCCACCGCTAAGAGTCGGATCGTC	5	0.125	No Hit
AGCACCACATCGATCTAAGAGATCGTCGGACTGTAGAACTC	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAAAAGAGCCCCCAGTTTGAGTGATCGTCGGACTGTAGAAC	5	0.125	No Hit
GCTTAGGCCCTGGCCCGAAGAGAACTCCGGAGCACCACATC	5	0.125	No Hit
CCAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
GCCCTGGCCCGAAGAGAACTCCGGAGCACCACATCGATCTA	5	0.125	No Hit
AATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGGATCG	5	0.125	No Hit
GATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACG	5	0.125	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCTTCTGACTCCAAGTCCAGTGATCGTCGGACTGTAGAAC	5	0.125	No Hit
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTC	5	0.125	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTACCTGCACTATAAGCACTTTAGATCGTCGGACTGTAGAA	5	0.125	No Hit
GACAACAAAATCACTAGTCTTCCAGATCGTCGGACTGTAGA	5	0.125	No Hit
GAAGAGAGCTTGCCCTTGCATAGATCGTCGGACTGTAGAAC	5	0.125	No Hit
ACTGAGGGGCCTCAGACCGAGCTGATCGTCGGACTGTAGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.55	0.0	0.0
2	0.0	0.0	1.6	0.0	0.0
3	0.0	0.0	1.6	0.0	0.0
4	0.0	0.0	1.6	0.0	0.0
5	0.0	0.0	1.625	0.0	0.0
6	0.0	0.0	1.625	0.0	0.0
7	0.0	0.0	1.625	0.0	0.0
8	0.0	0.0	1.675	0.0	0.0
9	0.0	0.0	1.9	0.0	0.0
10	0.0	0.0	2.625	0.0	0.0
11	0.0	0.0	3.175	0.0	0.0
12	0.0	0.0	3.925	0.0	0.0
13	0.0	0.0	5.075	0.0	0.0
14	0.0	0.0	6.225	0.0	0.0
15	0.0	0.0	7.375	0.0	0.0
16	0.0	0.0	8.625	0.0	0.0
17	0.0	0.0	9.8	0.0	0.0
18	0.0	0.0	10.825	0.0	0.0
19	0.0	0.0	12.075	0.0	0.0
20	0.0	0.0	13.525	0.0	0.0
21	0.0	0.0	16.375	0.0	0.0
22	0.0	0.0	27.825	0.0	0.0
23	0.0	0.0	49.15	0.0	0.0
24	0.0	0.0	57.725	0.0	0.0
25	0.0	0.0	63.325	0.0	0.0
26	0.0	0.0	65.575	0.0	0.0
27	0.0	0.0	66.7	0.0	0.0
28	0.0	0.0	67.7	0.0	0.0
29	0.0	0.0	69.625	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCCT	30	9.417061E-6	35.000004	3
GTGTCGA	30	9.417061E-6	35.000004	11
AGGGCTG	30	9.417061E-6	35.000004	17
CCTTGTG	30	9.417061E-6	35.000004	7
GTCGAGG	30	9.417061E-6	35.000004	13
CTGACTT	30	9.417061E-6	35.000004	21
GGCTGAC	30	9.417061E-6	35.000004	19
CGAGGGC	30	9.417061E-6	35.000004	15
GCTGACT	30	9.417061E-6	35.000004	20
CTTGTGT	30	9.417061E-6	35.000004	8
TTGTGTC	30	9.417061E-6	35.000004	9
CCCTTGT	30	9.417061E-6	35.000004	6
GAGGGCT	30	9.417061E-6	35.000004	16
CAAACCC	30	9.417061E-6	35.000004	2
TCGAGGG	30	9.417061E-6	35.000004	14
TGTGTCG	30	9.417061E-6	35.000004	10
TGTCGAG	30	9.417061E-6	35.000004	12
GGGCTGA	30	9.417061E-6	35.000004	18
CTACAAA	20	0.0021434545	35.0	4
ACAAACT	20	0.0021434545	35.0	6
>>END_MODULE
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
Written 180010 spots for SRR13079638.sra
Read 180010 spots for SRR13079638.sra
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SRR ids: ['SRR13079638.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_g0hz206d
SRR13079638.sra spots: 11520641
blocks: [[1, 180010], [180011, 360020], [360021, 540030], [540031, 720040], [720041, 900050], [900051, 1080060], [1080061, 1260070], [1260071, 1440080], [1440081, 1620090], [1620091, 1800100], [1800101, 1980110], [1980111, 2160120], [2160121, 2340130], [2340131, 2520140], [2520141, 2700150], [2700151, 2880160], [2880161, 3060170], [3060171, 3240180], [3240181, 3420190], [3420191, 3600200], [3600201, 3780210], [3780211, 3960220], [3960221, 4140230], [4140231, 4320240], [4320241, 4500250], [4500251, 4680260], [4680261, 4860270], [4860271, 5040280], [5040281, 5220290], [5220291, 5400300], [5400301, 5580310], [5580311, 5760320], [5760321, 5940330], [5940331, 6120340], [6120341, 6300350], [6300351, 6480360], [6480361, 6660370], [6660371, 6840380], [6840381, 7020390], [7020391, 7200400], [7200401, 7380410], [7380411, 7560420], [7560421, 7740430], [7740431, 7920440], [7920441, 8100450], [8100451, 8280460], [8280461, 8460470], [8460471, 8640480], [8640481, 8820490], [8820491, 9000500], [9000501, 9180510], [9180511, 9360520], [9360521, 9540530], [9540531, 9720540], [9720541, 9900550], [9900551, 10080560], [10080561, 10260570], [10260571, 10440580], [10440581, 10620590], [10620591, 10800600], [10800601, 10980610], [10980611, 11160620], [11160621, 11340630], [11340631, 11520641]]
SRR13079638 file size 1407132
SRR13079638 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR13079638 SRR13079638_1.fastq SRR13079638_2.fastq
Input file:	SRR13079638_1.fastq
Paired file:	SRR13079638_2.fastq
trimmed:	SRR13079638-trimmed-pair1.fastq, SRR13079638-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:42:38 2021 >> started

Tue Apr 20 16:42:58 2021 >> done (20.605s)
11520641 read pairs processed; of these:
 1516252 (13.16%) short read pairs filtered out after trimming by size control
  274597 ( 2.38%) empty read pairs filtered out after trimming by size control
 9729792 (84.46%) read pairs available; of these:
 8319124 (85.50%) trimmed read pairs available after processing
 1410668 (14.50%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 166196	  1.71%
 19	 152774	  1.57%
 20	 354771	  3.65%
 21	1362786	 14.01%
 22	2542264	 26.13%
 23	 925074	  9.51%
 24	 620552	  6.38%
 25	 273641	  2.81%
 26	 159036	  1.63%
 27	 144362	  1.48%
 28	 166452	  1.71%
 29	 142421	  1.46%
 30	 132704	  1.36%
 31	 185935	  1.91%
 32	 211029	  2.17%
 33	 153610	  1.58%
 34	 154487	  1.59%
 35	 105863	  1.09%
 36	  81693	  0.84%
 37	  82380	  0.85%
 38	  68864	  0.71%
 39	  66977	  0.69%
 40	  65253	  0.67%
 41	1410668	 14.50%
9729792 reads passed initial QC


criterion=sequence-density
sequence-density=7.44
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=4
prefix-density=0.01
prefix-fanout=1.0
sequence=ACTCAAACTGGGGGCTCTTTTG


criterion=fanout-score
sequence-density=0.83
sequence-density-rank=11
fanout-score=41.61
fanout-score-rank=1
prefix-density=0.99
prefix-fanout=35.0
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCT


criterion=sequence-density
sequence-density=3.05
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=ACACTACAAACTCTGCGGCACT


criterion=fanout-score
sequence-density=0.70
sequence-density-rank=7
fanout-score=35.40
fanout-score-rank=1
prefix-density=0.76
prefix-fanout=32.4
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 802: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 803: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x ACTCAAACTGGGGGCTCTTTTG -y ACACTACAAACTCTGCGGCACT -o SRR13079638 SRR13079638_1.fastq SRR13079638_2.fastq
Input file:	SRR13079638_1.fastq
Paired file:	SRR13079638_2.fastq
trimmed:	SRR13079638-trimmed-pair1.fastq, SRR13079638-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ACTCAAACTGGGGGCTCTTTTG
-- paired 3' end adapter sequence (-y):	ACACTACAAACTCTGCGGCACT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- number of concurrent threads (-t):	32
Tue Apr 20 16:43:28 2021 >> started

Tue Apr 20 16:43:38 2021 >> done (10.088s)
6486528 read pairs processed; of these:
   3223 ( 0.05%) short read pairs filtered out after trimming by size control
 825907 (12.73%) empty read pairs filtered out after trimming by size control
5657398 (87.22%) read pairs available; of these:
    150 ( 0.00%) trimmed read pairs available after processing
5657248 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 110102	  1.95%
 19	 100242	  1.77%
 20	 221165	  3.91%
 21	 560706	  9.91%
 22	1267501	 22.40%
 23	 594736	 10.51%
 24	 411694	  7.28%
 25	 181557	  3.21%
 26	 106009	  1.87%
 27	  96140	  1.70%
 28	 110978	  1.96%
 29	  94745	  1.67%
 30	  88398	  1.56%
 31	 124075	  2.19%
 32	 140415	  2.48%
 33	 102207	  1.81%
 34	 102790	  1.82%
 35	  70653	  1.25%
 36	  54507	  0.96%
 37	  54766	  0.97%
 38	  45416	  0.80%
 39	  44850	  0.79%
 40	  43249	  0.76%
 41	 930497	 16.45%


criterion=sequence-density
sequence-density=2.43
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=7
prefix-density=0.00
prefix-fanout=1.0
sequence=TTCAAGTAATCCAGGATAGGCTA


criterion=fanout-score
sequence-density=1.05
sequence-density-rank=10
fanout-score=44.77
fanout-score-rank=1
prefix-density=1.22
prefix-fanout=38.3
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCT


criterion=sequence-density
sequence-density=1.64
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=15
prefix-density=0.00
prefix-fanout=1.0
sequence=AGCCTATCCTGGATTACTTGAAGATCGTCGG


criterion=fanout-score
sequence-density=0.80
sequence-density-rank=4
fanout-score=42.51
fanout-score-rank=1
prefix-density=0.91
prefix-fanout=37.5
sequence=GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC
SRR13079638 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 20 16:44:52
                             Started mapping on |	Apr 20 16:44:53
                                    Finished on |	Apr 20 16:45:23
       Mapping speed, Million of reads per hour |	1068.08

                          Number of input reads |	8900662
                      Average input read length |	54
                                    UNIQUE READS:
                   Uniquely mapped reads number |	5943943
                        Uniquely mapped reads % |	66.78%
                          Average mapped length |	50.12
                       Number of splices: Total |	31803
            Number of splices: Annotated (sjdb) |	14569
                       Number of splices: GT/AG |	27521
                       Number of splices: GC/AG |	1179
                       Number of splices: AT/AC |	18
               Number of splices: Non-canonical |	3085
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.25
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1348166
             % of reads mapped to multiple loci |	15.15%
        Number of reads mapped to too many loci |	106633
             % of reads mapped to too many loci |	1.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	15.41%
                     % of reads unmapped: other |	1.46%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1608553	1608553	1608553
N_multimapping	1348166	1348166	1348166
N_noFeature	1198390	1323645	4274135
N_ambiguous	2286090	810990	399245
UnstrandedReadsAssigned:2459463 PositiveStrandReadsAssigned:3809308 NegativeStrandReadsAssigned:1270563
Dataset is classified unstranded
MeadianReadLen=23 20thPercentileLength=22 echo kmer=19
SRR13079638 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in paired-end mode
[quant] will process pair 1: SRR13079638-trimmed-pair1.fastq
                             SRR13079638-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 8,900,662 reads, 2,032,925 reads pseudoaligned
[quant] estimated average fragment length: 27.8457
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,252 rounds

 109283 SRR13079638.ke.tsv
  52636 SRR13079638.se.tsv
 161919 total
==> SRR13079638.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR13079638.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR13079638 completed mapping pipeline successfully
