Starting /dee2/code/volunteer_pipeline.sh SRR14016380
    current disk space = 320934996
    free memory = 263395160 
SRR14016380 SRAfilesize
5ec357dfe69d46ae0b4254933e0d4041  SRR14016380.sra
SRR14016380.sra file validated
SRR14016380 is paired end
SRR14016380 is conventional basespace
SRR14016380 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.6425	37.0	37.0	37.0	37.0	37.0
2	35.5485	37.0	37.0	37.0	37.0	37.0
3	35.614	37.0	37.0	37.0	37.0	37.0
4	35.8485	37.0	37.0	37.0	37.0	37.0
5	35.846	37.0	37.0	37.0	37.0	37.0
6	35.75325	37.0	37.0	37.0	37.0	37.0
7	35.712	37.0	37.0	37.0	37.0	37.0
8	35.941	37.0	37.0	37.0	37.0	37.0
9	35.7585	37.0	37.0	37.0	37.0	37.0
10-14	35.8183	37.0	37.0	37.0	37.0	37.0
15-19	35.6846	37.0	37.0	37.0	37.0	37.0
20-24	35.604600000000005	37.0	37.0	37.0	37.0	37.0
25-29	35.34830000000001	37.0	37.0	37.0	34.6	37.0
30-34	35.3202	37.0	37.0	37.0	37.0	37.0
35-39	34.9344	37.0	37.0	37.0	25.0	37.0
40-44	34.9424	37.0	37.0	37.0	25.0	37.0
45-49	34.7319	37.0	37.0	37.0	25.0	37.0
50-54	34.166000000000004	37.0	37.0	37.0	25.0	37.0
55-59	32.790099999999995	37.0	37.0	37.0	11.0	37.0
60-64	32.3262	37.0	37.0	37.0	11.0	37.0
65-69	31.8818	37.0	32.2	37.0	11.0	37.0
70-74	31.4646	37.0	25.0	37.0	11.0	37.0
75-79	31.5637	37.0	27.4	37.0	11.0	37.0
80-84	31.5734	37.0	25.0	37.0	11.0	37.0
85-89	30.901500000000006	37.0	25.0	37.0	11.0	37.0
90-94	30.6646	37.0	25.0	37.0	11.0	37.0
95-99	30.199099999999998	37.0	25.0	37.0	11.0	37.0
100-104	30.025150000000004	37.0	25.0	37.0	11.0	37.0
105-109	29.807100000000002	37.0	25.0	37.0	11.0	37.0
110-114	29.40605	37.0	19.4	37.0	11.0	37.0
115-119	29.186500000000002	37.0	16.6	37.0	11.0	37.0
120-124	29.149	37.0	16.6	37.0	11.0	37.0
125-129	28.7274	37.0	11.0	37.0	11.0	37.0
130-134	28.3315	37.0	11.0	37.0	11.0	37.0
135-139	28.1269	37.0	11.0	37.0	11.0	37.0
140-144	28.019299999999998	37.0	11.0	37.0	11.0	37.0
145-149	28.1784	37.0	11.0	37.0	11.0	37.0
150	27.9735	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	4.0
21	16.0
22	56.0
23	110.0
24	203.0
25	222.0
26	256.0
27	277.0
28	182.0
29	168.0
30	141.0
31	144.0
32	119.0
33	165.0
34	168.0
35	365.0
36	1294.0
37	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.0	29.425	14.625	25.95
2	15.825	27.725	32.45	24.0
3	25.525	21.7	31.225	21.55
4	20.05	34.675	19.15	26.125
5	20.424999999999997	42.725	20.3	16.55
6	28.132033008252062	26.531632908227053	28.80720180045011	16.52913228307077
7	22.75	22.725	30.925000000000004	23.599999999999998
8	29.425	29.125	22.875	18.575
9	26.174999999999997	22.525000000000002	33.875	17.424999999999997
10-14	19.735	30.404999999999998	27.51	22.35
15-19	24.04	27.42	25.545	22.994999999999997
20-24	24.185000000000002	28.42	30.28	17.115
25-29	18.740000000000002	25.25	35.3	20.71
30-34	19.869999999999997	24.884999999999998	37.845	17.4
35-39	17.09	24.525	40.53	17.854999999999997
40-44	18.17	23.595	42.089999999999996	16.145
45-49	17.575	24.044999999999998	42.19	16.189999999999998
50-54	18.029999999999998	25.535000000000004	39.895	16.54
55-59	20.78	27.544999999999998	33.375	18.3
60-64	20.349999999999998	31.240000000000002	30.23	18.18
65-69	19.965	32.115	29.885	18.035
70-74	20.474999999999998	33.129999999999995	28.749999999999996	17.645
75-79	23.73	32.875	24.625	18.77
80-84	22.235	33.61	24.58	19.575
85-89	20.810000000000002	35.465	22.62	21.105
90-94	20.855	35.83	22.5	20.815
95-99	19.950000000000003	36.845	21.39	21.815
100-104	18.4059202960148	38.846942347117356	20.256012800640033	22.491124556227813
105-109	18.8	37.980000000000004	22.035	21.185000000000002
110-114	18.013105897653944	39.702866289830425	21.224551047971588	21.059476764544044
115-119	18.955	38.3	21.67	21.075
120-124	19.855	36.975	21.98	21.19
125-129	19.655	36.855	21.44	22.05
130-134	20.04	37.525	21.67	20.765
135-139	21.44	36.93	20.200000000000003	21.43
140-144	23.044999999999998	37.605	20.415	18.935
145-149	24.915000000000003	35.335	20.845	18.905
150	27.725	32.85	21.6	17.825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	1.0
3	2.0
4	3.5
5	3.5
6	3.5
7	6.5
8	7.0
9	7.5
10	8.5
11	8.5
12	10.0
13	12.5
14	18.5
15	25.0
16	32.0
17	35.0
18	39.0
19	41.0
20	42.0
21	48.5
22	49.5
23	53.0
24	51.5
25	58.5
26	70.5
27	75.5
28	81.0
29	74.0
30	83.5
31	91.5
32	80.0
33	93.0
34	115.5
35	119.0
36	124.5
37	124.0
38	100.0
39	97.0
40	87.5
41	74.5
42	84.5
43	82.0
44	80.0
45	85.0
46	105.5
47	122.0
48	110.0
49	100.5
50	110.0
51	116.5
52	116.5
53	131.5
54	120.0
55	99.5
56	88.0
57	64.5
58	51.5
59	47.5
60	50.5
61	40.0
62	20.5
63	15.0
64	18.0
65	32.0
66	26.5
67	7.0
68	4.5
69	4.0
70	3.0
71	0.5
72	1.0
73	1.0
74	0.5
75	0.5
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.005
105-109	0.0
110-114	0.045
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12750116333177	39.825
2	14.890646812470917	16.0
3	4.792926942764077	7.725
4	2.559329920893439	5.5
5	0.9306654257794323	2.5
6	0.837598883201489	2.7
7	0.5118659841786878	1.925
8	0.09306654257794324	0.4
9	0.5118659841786878	2.475
>10	0.604932526756631	5.375
>50	0.04653327128897162	2.025
>100	0.09306654257794324	13.55
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	331	8.275	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	211	5.2749999999999995	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	81	2.025	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43	1.075	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	19	0.475	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.44999999999999996	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.42500000000000004	No Hit
GTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAA	15	0.375	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	15	0.375	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	15	0.375	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	14	0.35000000000000003	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	12	0.3	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	12	0.3	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	12	0.3	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	12	0.3	No Hit
GTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGA	11	0.27499999999999997	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	9	0.22499999999999998	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	9	0.22499999999999998	No Hit
CAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGC	9	0.22499999999999998	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	9	0.22499999999999998	No Hit
ATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTG	9	0.22499999999999998	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	9	0.22499999999999998	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	9	0.22499999999999998	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	9	0.22499999999999998	No Hit
CCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCT	9	0.22499999999999998	No Hit
ATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGT	9	0.22499999999999998	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	9	0.22499999999999998	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	8	0.2	No Hit
ATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAA	8	0.2	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	7	0.17500000000000002	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	7	0.17500000000000002	No Hit
CGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCA	7	0.17500000000000002	No Hit
ACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGG	7	0.17500000000000002	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	7	0.17500000000000002	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	7	0.17500000000000002	No Hit
CTTGTCTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTT	7	0.17500000000000002	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	7	0.17500000000000002	No Hit
CTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAAC	7	0.17500000000000002	No Hit
GTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGG	6	0.15	No Hit
GAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCC	6	0.15	No Hit
AGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATT	6	0.15	No Hit
GTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTG	6	0.15	No Hit
CTGGAAGGGGCAAGATAAGAATGCCCCCACAGAAGGTAACTTATATGTCT	6	0.15	No Hit
TAGCCAAGCAGGAACAAAGTCATGCTGCGCCATTTAGGTCCAGTGTCTTT	6	0.15	No Hit
GATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTT	6	0.15	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	6	0.15	No Hit
GTGAAAACGCCTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCA	6	0.15	No Hit
CTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	6	0.15	No Hit
GGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCC	6	0.15	No Hit
CTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCA	6	0.15	No Hit
ACAGAAGGTAACTTATATGTCTAAGAAGTATATTATCTACATTTGCTGTT	6	0.15	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	6	0.15	No Hit
TTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	6	0.15	No Hit
TTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCT	6	0.15	No Hit
GAAAACGCCTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACC	6	0.15	No Hit
AGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGT	6	0.15	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	5	0.125	No Hit
CGGCAGGGGCATCAAGGAAGTTGAATGCAGGGCCTTCCATAATTCTGGCA	5	0.125	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	5	0.125	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	5	0.125	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	5	0.125	No Hit
CTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCG	5	0.125	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	5	0.125	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	5	0.125	No Hit
GCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAG	5	0.125	No Hit
CTTGAATGGGCAACTACAGTGATGTGGGTCCCTTGCCTTTCAATTTTGGC	5	0.125	No Hit
CTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACCAGACCAGC	5	0.125	No Hit
GCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCG	5	0.125	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	5	0.125	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	5	0.125	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	5	0.125	No Hit
TACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGC	5	0.125	No Hit
TCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGA	5	0.125	No Hit
TTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGA	5	0.125	No Hit
TTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTG	5	0.125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0125	0.0	0.0	0.0	0.0
32-33	0.037500000000000006	0.0	0.0	0.0	0.0
34-35	0.05	0.0	0.0	0.0	0.0
36-37	0.1125	0.0	0.0	0.0	0.0
38-39	0.16249999999999998	0.0	0.0	0.0	0.0
40-41	0.2625	0.0	0.0	0.0	0.0
42-43	0.3375	0.0	0.0	0.0	0.0
44-45	0.3875	0.0	0.0	0.0	0.0
46-47	0.4375	0.0	0.0	0.0	0.0
48-49	0.5375000000000001	0.0	0.0	0.0	0.0
50-51	0.7	0.0	0.0	0.0	0.0
52-53	0.85	0.0	0.0	0.0	0.0
54-55	0.9625	0.0	0.0	0.0	0.0
56-57	1.2000000000000002	0.0	0.0	0.0	0.0
58-59	1.425	0.0	0.0	0.0	0.0
60-61	1.7374999999999998	0.0	0.0	0.0	0.0
62-63	2.0625	0.0	0.0	0.0	0.0
64-65	2.5374999999999996	0.0	0.0	0.0	0.0
66-67	2.95	0.0	0.0	0.0	0.0
68-69	3.525	0.0	0.0	0.0	0.0
70-71	4.125	0.0	0.0	0.0	0.0
72-73	4.8375	0.0	0.0	0.0	0.0
74-75	5.7875	0.0	0.0	0.0	0.0
76-77	6.65	0.0	0.0	0.0	0.0
78-79	7.5125	0.0	0.0	0.0	0.0
80-81	8.45	0.0	0.0	0.0	0.0
82-83	9.225	0.0	0.0	0.0	0.0
84-85	10.175	0.0	0.0	0.0	0.0
86-87	10.825	0.0	0.0	0.0	0.0
88-89	11.85	0.0	0.0	0.0	0.0
90-91	12.775	0.0	0.0	0.0	0.0
92-93	14.0125	0.0	0.0	0.0	0.0
94-95	15.0625	0.0	0.0	0.0	0.0
96-97	16.325	0.0	0.0	0.0	0.0
98-99	17.425	0.0	0.0	0.0	0.0
100-101	18.1	0.0	0.0	0.0	0.0
102-103	18.775	0.0	0.0	0.0	0.0
104-105	19.475	0.0	0.0	0.0	0.0
106-107	20.1625	0.0	0.0	0.0	0.0
108-109	20.9125	0.0	0.0	0.0	0.0
110-111	21.55	0.0	0.0	0.0	0.0
112-113	22.262500000000003	0.0	0.0	0.0	0.0
114-115	22.9125	0.0	0.0	0.0	0.0
116-117	23.725	0.0	0.0	0.0	0.0
118-119	24.4625	0.0	0.0	0.0	0.0
120-121	25.2375	0.0	0.0	0.0	0.0
122-123	26.25	0.0	0.0	0.0	0.0
124-125	26.95	0.0	0.0	0.0	0.0
126-127	27.525	0.0	0.0	0.0	0.0
128-129	28.275	0.0	0.0	0.0	0.0
130-131	29.1875	0.0	0.0	0.0	0.0
132-133	29.9	0.0	0.0	0.0	0.0
134-135	30.7125	0.0	0.0	0.0	0.0
136-137	31.362499999999997	0.0	0.0	0.0	0.0
138	31.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	30	5.2386895E-10	144.0	1
CTAATCA	30	5.2386895E-10	144.0	2
TAATCAT	30	5.2386895E-10	144.0	3
AATCATT	35	1.5315891E-9	123.428566	4
ATCATTG	35	1.5315891E-9	123.428566	5
TCATTGC	35	1.5315891E-9	123.428566	6
TTGCAAG	40	3.8780854E-9	108.0	9
CATTGCA	40	3.8780854E-9	108.0	7
ATTGCAA	45	8.794814E-9	96.0	8
AAGCAGT	70	1.8854553E-7	61.714283	1
AGCAGTG	90	1.0710228E-6	48.0	2
TGGTATC	100	2.2145523E-6	43.199997	7
AGTGGTA	100	2.2145523E-6	43.199997	5
GTATCAA	100	2.2145523E-6	43.199997	9
GCAGTGG	100	2.2145523E-6	43.199997	3
GTGGTAT	100	2.2145523E-6	43.199997	6
CAGTGGT	100	2.2145523E-6	43.199997	4
GGTATCA	100	2.2145523E-6	43.199997	8
TATCAAC	100	0.005573872	28.8	9
GCAAGCA	40	3.1003024E-4	21.599998	10-14
>>END_MODULE
SRR14016380 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016380_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.707	37.0	37.0	37.0	25.0	37.0
2	34.618	37.0	37.0	37.0	25.0	37.0
3	35.0565	37.0	37.0	37.0	25.0	37.0
4	35.119	37.0	37.0	37.0	25.0	37.0
5	35.284	37.0	37.0	37.0	37.0	37.0
6	35.1965	37.0	37.0	37.0	37.0	37.0
7	35.2195	37.0	37.0	37.0	37.0	37.0
8	35.2405	37.0	37.0	37.0	37.0	37.0
9	35.287	37.0	37.0	37.0	37.0	37.0
10-14	35.355399999999996	37.0	37.0	37.0	37.0	37.0
15-19	35.326800000000006	37.0	37.0	37.0	34.6	37.0
20-24	35.204699999999995	37.0	37.0	37.0	32.2	37.0
25-29	35.0343	37.0	37.0	37.0	27.4	37.0
30-34	34.994600000000005	37.0	37.0	37.0	25.0	37.0
35-39	34.7478	37.0	37.0	37.0	25.0	37.0
40-44	34.7307	37.0	37.0	37.0	25.0	37.0
45-49	34.40259999999999	37.0	37.0	37.0	25.0	37.0
50-54	33.8395	37.0	37.0	37.0	22.2	37.0
55-59	32.3405	37.0	37.0	37.0	11.0	37.0
60-64	32.1128	37.0	37.0	37.0	11.0	37.0
65-69	31.6382	37.0	29.8	37.0	11.0	37.0
70-74	31.1997	37.0	25.0	37.0	11.0	37.0
75-79	31.1941	37.0	25.0	37.0	11.0	37.0
80-84	31.036700000000003	37.0	25.0	37.0	11.0	37.0
85-89	30.4988	37.0	25.0	37.0	11.0	37.0
90-94	30.1826	37.0	25.0	37.0	11.0	37.0
95-99	29.763799999999996	37.0	25.0	37.0	11.0	37.0
100-104	29.7349	37.0	25.0	37.0	11.0	37.0
105-109	29.436	37.0	22.2	37.0	11.0	37.0
110-114	29.104300000000002	37.0	13.8	37.0	11.0	37.0
115-119	28.721899999999998	37.0	11.0	37.0	11.0	37.0
120-124	28.3324	37.0	11.0	37.0	11.0	37.0
125-129	27.980199999999996	37.0	11.0	37.0	11.0	37.0
130-134	27.8572	37.0	11.0	37.0	11.0	37.0
135-139	27.550300000000004	37.0	11.0	37.0	11.0	37.0
140-144	27.2103	37.0	11.0	37.0	11.0	37.0
145-149	27.272399999999998	37.0	11.0	37.0	11.0	37.0
150	27.134	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	9.0
20	13.0
21	41.0
22	73.0
23	154.0
24	205.0
25	289.0
26	258.0
27	206.0
28	169.0
29	97.0
30	141.0
31	127.0
32	157.0
33	189.0
34	277.0
35	557.0
36	1000.0
37	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.004004004004003	29.52952952952953	14.014014014014014	27.45245245245245
2	15.8	29.075	31.125000000000004	24.0
3	25.95	22.775000000000002	30.875000000000004	20.4
4	19.7	31.724999999999998	20.325	28.249999999999996
5	20.525	42.675000000000004	19.2	17.599999999999998
6	28.749999999999996	27.650000000000002	25.674999999999997	17.925
7	21.125	23.25	33.475	22.15
8	30.349999999999998	28.525	22.025	19.1
9	29.025000000000002	21.575	31.4	18.0
10-14	20.07	30.225	27.150000000000002	22.555
15-19	23.845	27.755000000000003	25.19	23.21
20-24	24.305	27.950000000000003	30.025000000000002	17.72
25-29	19.175	25.180000000000003	35.015	20.630000000000003
30-34	20.544999999999998	24.385	37.55	17.52
35-39	16.895	23.525	41.385	18.195
40-44	18.605	23.080000000000002	41.94	16.375
45-49	18.655	24.25	40.955000000000005	16.14
50-54	19.009999999999998	26.040000000000003	38.065	16.885
55-59	21.224999999999998	27.425	32.96	18.39
60-64	20.125	31.830000000000002	29.975	18.07
65-69	20.68	31.7	30.25	17.37
70-74	21.404999999999998	31.790000000000003	29.525000000000002	17.28
75-79	24.044999999999998	31.805	26.33	17.82
80-84	24.08	32.71	25.259999999999998	17.95
85-89	23.1	35.265	23.505000000000003	18.13
90-94	23.549999999999997	36.63	23.075000000000003	16.744999999999997
95-99	23.974999999999998	36.855	22.67	16.5
100-104	23.805	38.824999999999996	21.15	16.220000000000002
105-109	24.52	37.88	22.900000000000002	14.7
110-114	24.68	39.58	21.355	14.385
115-119	26.939999999999998	38.14	21.425	13.495
120-124	27.200000000000003	37.57	21.02	14.21
125-129	26.595000000000002	37.455	21.584999999999997	14.365
130-134	27.925	37.714999999999996	20.74	13.62
135-139	30.445	36.935	19.17	13.450000000000001
140-144	32.525	36.22	19.895	11.360000000000001
145-149	34.64	34.93	19.794999999999998	10.635
150	36.975	32.925	20.025000000000002	10.075000000000001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	3.0
2	3.0
3	2.0
4	1.5
5	1.5
6	3.5
7	7.0
8	9.0
9	8.5
10	10.0
11	12.5
12	13.0
13	23.0
14	31.5
15	27.5
16	25.5
17	32.0
18	41.0
19	41.5
20	48.0
21	56.5
22	57.5
23	51.0
24	48.5
25	61.0
26	64.5
27	63.0
28	69.0
29	82.0
30	90.0
31	76.5
32	65.0
33	79.0
34	100.5
35	99.5
36	92.5
37	101.0
38	97.5
39	94.0
40	88.0
41	90.5
42	89.5
43	83.0
44	84.5
45	88.0
46	104.0
47	121.0
48	128.5
49	111.0
50	113.0
51	118.0
52	123.0
53	140.5
54	121.5
55	104.5
56	89.5
57	67.5
58	57.0
59	52.5
60	52.5
61	44.0
62	26.5
63	12.5
64	19.5
65	29.0
66	20.5
67	8.0
68	3.0
69	4.0
70	4.0
71	1.0
72	0.5
73	0.5
74	1.5
75	1.0
76	0.0
77	0.5
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.425000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45414266725741	42.575
2	14.133805937084626	15.950000000000001
3	4.297740363314134	7.2749999999999995
4	2.3482498892334958	5.3
5	1.2405848471422243	3.5000000000000004
6	0.7532122286220646	2.55
7	0.35445281346920693	1.4000000000000001
8	0.48737261852015945	2.1999999999999997
9	0.17722640673460346	0.8999999999999999
>10	0.6202924235711121	4.9750000000000005
>50	0.044306601683650866	1.3
>100	0.08861320336730173	12.075
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	320	8.0	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	163	4.075	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	52	1.3	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.8750000000000001	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.8250000000000001	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	12	0.3	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	12	0.3	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	11	0.27499999999999997	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	11	0.27499999999999997	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	11	0.27499999999999997	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	11	0.27499999999999997	No Hit
AATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTG	11	0.27499999999999997	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	10	0.25	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	10	0.25	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	10	0.25	No Hit
AGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGT	9	0.22499999999999998	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	9	0.22499999999999998	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	8	0.2	No Hit
GTTTCGGGAGATGTGTGAGGGCCTGCTGGAGGAGTCTGATGGTGAGGATG	8	0.2	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	8	0.2	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	8	0.2	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	8	0.2	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	8	0.2	No Hit
CTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGC	8	0.2	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	8	0.2	No Hit
CCCTCTTTCACTGGAAGGGGCAAGATAAGAATGCCCCCACAGAAGGTAAC	8	0.2	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	8	0.2	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGACA	7	0.17500000000000002	No Hit
CTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCG	7	0.17500000000000002	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGTAGAG	7	0.17500000000000002	No Hit
CTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGT	7	0.17500000000000002	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	7	0.17500000000000002	No Hit
CTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATG	7	0.17500000000000002	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	6	0.15	No Hit
AAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCG	6	0.15	No Hit
AAGCAGTGGTATCAACGCAGAGTACTGTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCA	6	0.15	No Hit
AGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATT	6	0.15	No Hit
GCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAG	6	0.15	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	6	0.15	No Hit
CGGCAACTTTGGATACAGGACCACAGAGGTGACACTCTGAGGATCCTGGC	6	0.15	No Hit
CTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTG	6	0.15	No Hit
GAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCC	6	0.15	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	6	0.15	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	6	0.15	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	6	0.15	No Hit
CTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	6	0.15	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	6	0.15	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	5	0.125	No Hit
CGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGA	5	0.125	No Hit
CTTACGCAAAGGTCCTAGAAGACAACTCCGTACCTCAGGTCAAAGACATC	5	0.125	No Hit
TGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAG	5	0.125	No Hit
AAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGAT	5	0.125	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	5	0.125	No Hit
CTTGTCTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTT	5	0.125	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GATTTGTGCCAGAATTATGGAAGGCCCTGCATTCAACTTCCTTGATGCCC	5	0.125	No Hit
GAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAG	5	0.125	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	5	0.125	No Hit
CTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACCAGACCAGC	5	0.125	No Hit
GTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATA	5	0.125	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	5	0.125	No Hit
TAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCT	5	0.125	No Hit
AAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGA	5	0.125	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	5	0.125	No Hit
CCTCTTTCACTGGAAGGGGCAAGATAAGAATGCCCCCACAGAAGGTAACT	5	0.125	No Hit
AGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTG	5	0.125	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACCAGACCAGCCA	5	0.125	No Hit
GACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACG	5	0.125	No Hit
AGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGA	5	0.125	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	5	0.125	No Hit
GCCCTCTTTCACTGGAAGGGGCAAGATAAGAATGCCCCCACAGAAGGTAA	5	0.125	No Hit
AGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGT	5	0.125	No Hit
TGAAAACGCCTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.037500000000000006	0.0	0.0	0.0	0.0
32-33	0.0625	0.0	0.0	0.0	0.0
34-35	0.0875	0.0	0.0	0.0	0.0
36-37	0.16249999999999998	0.0	0.0	0.0	0.0
38-39	0.21250000000000002	0.0	0.0	0.0	0.0
40-41	0.3125	0.0	0.0	0.0	0.0
42-43	0.4	0.0	0.0	0.0	0.0
44-45	0.4625	0.0	0.0	0.0	0.0
46-47	0.5125	0.0	0.0	0.0	0.0
48-49	0.6375	0.0	0.0	0.0	0.0
50-51	0.825	0.0	0.0	0.0	0.0
52-53	0.975	0.0	0.0	0.0	0.0
54-55	1.0875	0.0	0.0	0.0	0.0
56-57	1.2999999999999998	0.0	0.0	0.0	0.0
58-59	1.525	0.0	0.0	0.0	0.0
60-61	1.8624999999999998	0.0	0.0	0.0	0.0
62-63	2.2125	0.0	0.0	0.0	0.0
64-65	2.65	0.0	0.0	0.0	0.0
66-67	3.0125	0.0	0.0	0.0	0.0
68-69	3.5875000000000004	0.0	0.0	0.0	0.0
70-71	4.1625	0.0	0.0	0.0	0.0
72-73	4.8125	0.0	0.0	0.0	0.0
74-75	5.775	0.0	0.0	0.0	0.0
76-77	6.55	0.0	0.0	0.0	0.0
78-79	7.375	0.0	0.0	0.0	0.0
80-81	8.2375	0.0	0.0	0.0	0.0
82-83	8.975	0.0	0.0	0.0	0.0
84-85	9.875	0.0	0.0	0.0	0.0
86-87	10.6125	0.0	0.0	0.0	0.0
88-89	11.675	0.0	0.0	0.0	0.0
90-91	12.587499999999999	0.0	0.0	0.0	0.0
92-93	13.7125	0.0	0.0	0.0	0.0
94-95	14.7	0.0	0.0	0.0	0.0
96-97	15.9375	0.0	0.0	0.0	0.0
98-99	17.0375	0.0	0.0	0.0	0.0
100-101	17.7375	0.0	0.0	0.0	0.0
102-103	18.3625	0.0	0.0	0.0	0.0
104-105	19.0125	0.0	0.0	0.0	0.0
106-107	19.6	0.0	0.0	0.0	0.0
108-109	20.35	0.0	0.0	0.0	0.0
110-111	20.9	0.0	0.0	0.0	0.0
112-113	21.5125	0.0	0.0	0.0	0.0
114-115	22.1625	0.0	0.0	0.0	0.0
116-117	22.924999999999997	0.0	0.0	0.0	0.0
118-119	23.625	0.0	0.0	0.0	0.0
120-121	24.4	0.0	0.0	0.0	0.0
122-123	25.424999999999997	0.0	0.0	0.0	0.0
124-125	26.1	0.0	0.0	0.0	0.0
126-127	26.6375	0.0	0.0	0.0	0.0
128-129	27.3375	0.0	0.0	0.0	0.0
130-131	28.175	0.0	0.0	0.0	0.0
132-133	28.8	0.0	0.0	0.0	0.0
134-135	29.6	0.0	0.0	0.0	0.0
136-137	30.225	0.0	0.0	0.0	0.0
138	30.75	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCA	15	1.1730364E-4	144.0	7
AATCATT	15	1.1730364E-4	144.0	4
CGGGGCT	10	0.006973645	144.0	9
GCTAATC	15	1.1730364E-4	144.0	1
ATTGCAA	15	1.1730364E-4	144.0	8
CTAATCA	15	1.1730364E-4	144.0	2
TAATCAT	15	1.1730364E-4	144.0	3
TCATTGC	15	1.1730364E-4	144.0	6
TTGCAAG	20	3.687869E-4	108.0	9
ATCATTG	20	3.687869E-4	108.0	5
AAGCAGT	55	3.092282E-10	91.63637	1
AGCAGTG	65	1.1623342E-9	77.53846	2
TGGTATC	75	3.610694E-9	67.2	7
AGTGGTA	75	3.610694E-9	67.2	5
GTATCAA	75	3.610694E-9	67.2	9
GTGGTAT	75	3.610694E-9	67.2	6
CAGTGGT	75	3.610694E-9	67.2	4
GGTATCA	75	3.610694E-9	67.2	8
GCAGTGG	85	9.71886E-9	59.294117	3
ATCAACG	90	2.1087119E-5	24.0	9
>>END_MODULE
2023-05-11T04:20:16 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000068.7 as a RefSeq or as a WGS
2023-05-11T04:20:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:20:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:20:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:20:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:20:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:20:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:20:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:27 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016380.sra
2023-05-11T04:21:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
2023-05-11T04:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T04:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
Read 944459 spots for SRR14016380.sra
Written 944459 spots for SRR14016380.sra
2023-05-11T04:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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SRR ids: ['SRR14016380.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bzidvb3g
SRR14016380.sra spots: 60445406
blocks: [[1, 944459], [944460, 1888918], [1888919, 2833377], [2833378, 3777836], [3777837, 4722295], [4722296, 5666754], [5666755, 6611213], [6611214, 7555672], [7555673, 8500131], [8500132, 9444590], [9444591, 10389049], [10389050, 11333508], [11333509, 12277967], [12277968, 13222426], [13222427, 14166885], [14166886, 15111344], [15111345, 16055803], [16055804, 17000262], [17000263, 17944721], [17944722, 18889180], [18889181, 19833639], [19833640, 20778098], [20778099, 21722557], [21722558, 22667016], [22667017, 23611475], [23611476, 24555934], [24555935, 25500393], [25500394, 26444852], [26444853, 27389311], [27389312, 28333770], [28333771, 29278229], [29278230, 30222688], [30222689, 31167147], [31167148, 32111606], [32111607, 33056065], [33056066, 34000524], [34000525, 34944983], [34944984, 35889442], [35889443, 36833901], [36833902, 37778360], [37778361, 38722819], [38722820, 39667278], [39667279, 40611737], [40611738, 41556196], [41556197, 42500655], [42500656, 43445114], [43445115, 44389573], [44389574, 45334032], [45334033, 46278491], [46278492, 47222950], [47222951, 48167409], [48167410, 49111868], [49111869, 50056327], [50056328, 51000786], [51000787, 51945245], [51945246, 52889704], [52889705, 53834163], [53834164, 54778622], [54778623, 55723081], [55723082, 56667540], [56667541, 57611999], [57612000, 58556458], [58556459, 59500917], [59500918, 60445406]]
fastq-dump error! exit code: 3
SRR14016380 file size 12424136
SRR14016380 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016380 SRR14016380_1.fastq SRR14016380_2.fastq
Input file:	SRR14016380_1.fastq
Paired file:	SRR14016380_2.fastq
trimmed:	SRR14016380-trimmed-pair1.fastq, SRR14016380-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 04:23:03 2023 >> started

Thu May 11 04:24:26 2023 >> done (82.813s)
36833901 read pairs processed; of these:
     735 ( 0.00%) short read pairs filtered out after trimming by size control
   11542 ( 0.03%) empty read pairs filtered out after trimming by size control
36821624 (99.97%) read pairs available; of these:
11585944 (31.47%) trimmed read pairs available after processing
25235680 (68.53%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      84	  0.00%
 19	      93	  0.00%
 20	     175	  0.00%
 21	     317	  0.00%
 22	     790	  0.00%
 23	     846	  0.00%
 24	     954	  0.00%
 25	    1245	  0.00%
 26	    1752	  0.00%
 27	    2025	  0.01%
 28	    2965	  0.01%
 29	    4364	  0.01%
 30	    5811	  0.02%
 31	    8032	  0.02%
 32	    9662	  0.03%
 33	   10126	  0.03%
 34	   11340	  0.03%
 35	   11107	  0.03%
 36	   10594	  0.03%
 37	   12230	  0.03%
 38	   12738	  0.03%
 39	   14375	  0.04%
 40	   14533	  0.04%
 41	   15540	  0.04%
 42	   16261	  0.04%
 43	   15503	  0.04%
 44	   16406	  0.04%
 45	   18247	  0.05%
 46	   21386	  0.06%
 47	   21954	  0.06%
 48	   21884	  0.06%
 49	   23803	  0.06%
 50	   25142	  0.07%
 51	   27903	  0.08%
 52	   26144	  0.07%
 53	   25069	  0.07%
 54	   29001	  0.08%
 55	   31975	  0.09%
 56	   32484	  0.09%
 57	   34122	  0.09%
 58	   35781	  0.10%
 59	   36655	  0.10%
 60	   39375	  0.11%
 61	   40145	  0.11%
 62	   41805	  0.11%
 63	   44230	  0.12%
 64	   45940	  0.12%
 65	   48051	  0.13%
 66	   49907	  0.14%
 67	   51162	  0.14%
 68	   53612	  0.15%
 69	   55330	  0.15%
 70	   57394	  0.16%
 71	   59252	  0.16%
 72	   62042	  0.17%
 73	   64730	  0.18%
 74	   66529	  0.18%
 75	   68889	  0.19%
 76	   71238	  0.19%
 77	   72540	  0.20%
 78	   74026	  0.20%
 79	   76636	  0.21%
 80	   78050	  0.21%
 81	   80129	  0.22%
 82	   82705	  0.22%
 83	   84681	  0.23%
 84	   87517	  0.24%
 85	   89765	  0.24%
 86	   91843	  0.25%
 87	   92708	  0.25%
 88	   95648	  0.26%
 89	   97416	  0.26%
 90	   99501	  0.27%
 91	  101532	  0.28%
 92	  103944	  0.28%
 93	  105240	  0.29%
 94	  107135	  0.29%
 95	  110156	  0.30%
 96	  111585	  0.30%
 97	  113890	  0.31%
 98	  116065	  0.32%
 99	  117933	  0.32%
100	  119780	  0.33%
101	  121407	  0.33%
102	  123372	  0.34%
103	  125297	  0.34%
104	  128024	  0.35%
105	  130080	  0.35%
106	  131509	  0.36%
107	  133510	  0.36%
108	  134150	  0.36%
109	  136419	  0.37%
110	  138893	  0.38%
111	  139949	  0.38%
112	  141112	  0.38%
113	  142974	  0.39%
114	  144888	  0.39%
115	  147227	  0.40%
116	  149007	  0.40%
117	  150267	  0.41%
118	  152140	  0.41%
119	  152213	  0.41%
120	  153111	  0.42%
121	  155942	  0.42%
122	  156050	  0.42%
123	  158331	  0.43%
124	  160116	  0.43%
125	  161833	  0.44%
126	  161370	  0.44%
127	  164156	  0.45%
128	  165685	  0.45%
129	  166072	  0.45%
130	  167443	  0.45%
131	  167187	  0.45%
132	  168806	  0.46%
133	  170316	  0.46%
134	  171072	  0.46%
135	  171934	  0.47%
136	  173504	  0.47%
137	  174758	  0.47%
138	  174444	  0.47%
139	  175870	  0.48%
140	  177118	  0.48%
141	  177057	  0.48%
142	  178216	  0.48%
143	  179075	  0.49%
144	  177393	  0.48%
145	  179358	  0.49%
146	  179452	  0.49%
147	  181382	  0.49%
148	  182921	  0.50%
149	  192060	  0.52%
150	25235680	 68.53%
36821624 reads passed initial QC


criterion=sequence-density
sequence-density=4.29
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=38
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=35
fanout-score=38.89
fanout-score-rank=1
prefix-density=0.53
prefix-fanout=1.2
sequence=GTGGAGGGCAGTGCCTTAGTGCAGTGTTGTCATAGTTAAGCAATTTCACTTTCCTTTTTCTCCTCTTTGGAGGCCCCTGAAGGAGTTCTCTCTGGGCTTCCCAGAGACTCAGAGGGGGTTCGGTCTACCCTGAACAGGGTCCCTTGGAGTCTGTGTAGGGGATGGCAGGAGGCTACAATGAACTCAGCAGCAGGACAATCCCAGAGGACCAGAATGAAGAGGAGTCAGATGACTATCAGCTCATGTTCAAGCACTTCAAAGAAAACAAGGTGGAGATCGCAAGTGCCATCACCAAGCCGTTTCCTTTCCTCATGAGCCTTCGAGACCGAGACTTCATCTCGGAGCAGAAGTTTCAGGAATATCAAGAAACATGTAAGAACCTGGTCCCAGTGGAGAGAGTCGTGTACGACATCCTCAGCAAT


criterion=sequence-density
sequence-density=2.22
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=31
prefix-density=2.24
prefix-fanout=2.0
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=63.80
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=5.2
sequence=ATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATCG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG -y TTATTGGATCAACA -o SRR14016380 SRR14016380_1.fastq SRR14016380_2.fastq
Input file:	SRR14016380_1.fastq
Paired file:	SRR14016380_2.fastq
trimmed:	SRR14016380-trimmed-pair1.fastq, SRR14016380-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG
-- paired 3' end adapter sequence (-y):	TTATTGGATCAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 04:28:38 2023 >> started

Thu May 11 04:29:20 2023 >> done (41.923s)
18410812 read pairs processed; of these:
      94 ( 0.00%) short read pairs filtered out after trimming by size control
  465477 ( 2.53%) empty read pairs filtered out after trimming by size control
17945241 (97.47%) read pairs available; of these:
    3047 ( 0.02%) trimmed read pairs available after processing
17942194 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      48	  0.00%
 19	      48	  0.00%
 20	      92	  0.00%
 21	     139	  0.00%
 22	     401	  0.00%
 23	     453	  0.00%
 24	     509	  0.00%
 25	     608	  0.00%
 26	     897	  0.00%
 27	     987	  0.01%
 28	    1491	  0.01%
 29	    2166	  0.01%
 30	    2935	  0.02%
 31	    3975	  0.02%
 32	    4903	  0.03%
 33	    4962	  0.03%
 34	    5412	  0.03%
 35	    5197	  0.03%
 36	    5378	  0.03%
 37	    5947	  0.03%
 38	    6347	  0.04%
 39	    7075	  0.04%
 40	    7207	  0.04%
 41	    7663	  0.04%
 42	    7696	  0.04%
 43	    7627	  0.04%
 44	    8195	  0.05%
 45	    9040	  0.05%
 46	   10811	  0.06%
 47	   11091	  0.06%
 48	   11075	  0.06%
 49	   11415	  0.06%
 50	   12258	  0.07%
 51	   13226	  0.07%
 52	   12815	  0.07%
 53	   12513	  0.07%
 54	   14272	  0.08%
 55	   16374	  0.09%
 56	   15765	  0.09%
 57	   16630	  0.09%
 58	   17515	  0.10%
 59	   18164	  0.10%
 60	   19323	  0.11%
 61	   19519	  0.11%
 62	   20291	  0.11%
 63	   21323	  0.12%
 64	   22510	  0.13%
 65	   23694	  0.13%
 66	   24369	  0.14%
 67	   24987	  0.14%
 68	   26492	  0.15%
 69	   27192	  0.15%
 70	   28090	  0.16%
 71	   28853	  0.16%
 72	   30387	  0.17%
 73	   31525	  0.18%
 74	   32764	  0.18%
 75	   33488	  0.19%
 76	   34547	  0.19%
 77	   35300	  0.20%
 78	   36364	  0.20%
 79	   37576	  0.21%
 80	   37891	  0.21%
 81	   39007	  0.22%
 82	   40568	  0.23%
 83	   41276	  0.23%
 84	   42959	  0.24%
 85	   43092	  0.24%
 86	   45229	  0.25%
 87	   45562	  0.25%
 88	   46823	  0.26%
 89	   48086	  0.27%
 90	   48360	  0.27%
 91	   49175	  0.27%
 92	   50417	  0.28%
 93	   50990	  0.28%
 94	   52332	  0.29%
 95	   53279	  0.30%
 96	   54576	  0.30%
 97	   55578	  0.31%
 98	   56772	  0.32%
 99	   57266	  0.32%
100	   59278	  0.33%
101	   60036	  0.33%
102	   60629	  0.34%
103	   61353	  0.34%
104	   61904	  0.34%
105	   63690	  0.35%
106	   64487	  0.36%
107	   66003	  0.37%
108	   65447	  0.36%
109	   66806	  0.37%
110	   67758	  0.38%
111	   67899	  0.38%
112	   69191	  0.39%
113	   70171	  0.39%
114	   70882	  0.39%
115	   71752	  0.40%
116	   73072	  0.41%
117	   73649	  0.41%
118	   73919	  0.41%
119	   73635	  0.41%
120	   74540	  0.42%
121	   76054	  0.42%
122	   76488	  0.43%
123	   77282	  0.43%
124	   77869	  0.43%
125	   78236	  0.44%
126	   78491	  0.44%
127	   79070	  0.44%
128	   80833	  0.45%
129	   80949	  0.45%
130	   81594	  0.45%
131	   81448	  0.45%
132	   82444	  0.46%
133	   82930	  0.46%
134	   83474	  0.47%
135	   84087	  0.47%
136	   84365	  0.47%
137	   85523	  0.48%
138	   85589	  0.48%
139	   85930	  0.48%
140	   86500	  0.48%
141	   86933	  0.48%
142	   87014	  0.48%
143	   87434	  0.49%
144	   86416	  0.48%
145	   87634	  0.49%
146	   87470	  0.49%
147	   89037	  0.50%
148	   89886	  0.50%
149	   92636	  0.52%
150	12286370	 68.47%


criterion=sequence-density
sequence-density=1.19
sequence-density-rank=1
fanout-score=32.60
fanout-score-rank=2
prefix-density=1.21
prefix-fanout=32.0
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=31
fanout-score=80.86
fanout-score-rank=1
prefix-density=0.18
prefix-fanout=14.4
sequence=AGGAGAAGGAGGTGGGGAGGAGGTTTGGAGCACCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACATTGATGTCTTTGGGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGCATCAGCCCGTTTGATTTCCAGCTTGGTGCCTCCACCGAACGTCGGAGGAGTACTTAACACAT


criterion=sequence-density
sequence-density=3.37
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=43
prefix-density=0.01
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=41
fanout-score=65.61
fanout-score-rank=1
prefix-density=0.16
prefix-fanout=11.1
sequence=AGGAGAAGGAGGTGGGGAGGAGGTTTGGAGCACCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACATTGATGTCTTTGGGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGCATCAGCCCGTTTGATTTCCAGCTTGGTGCCTCCACCGAACGTCGGAGGAGTACTTAACACAT
SRR14016380 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 11 04:32:17
                             Started mapping on |	May 11 04:32:17
                                    Finished on |	May 11 04:37:54
       Mapping speed, Million of reads per hour |	388.37

                          Number of input reads |	36356053
                      Average input read length |	275
                                    UNIQUE READS:
                   Uniquely mapped reads number |	28971476
                        Uniquely mapped reads % |	79.69%
                          Average mapped length |	268.43
                       Number of splices: Total |	27985943
            Number of splices: Annotated (sjdb) |	27387417
                       Number of splices: GT/AG |	27600759
                       Number of splices: GC/AG |	221357
                       Number of splices: AT/AC |	38650
               Number of splices: Non-canonical |	125177
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.78
                        Insertion rate per base |	0.01%
                       Insertion average length |	2.76
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1949274
             % of reads mapped to multiple loci |	5.36%
        Number of reads mapped to too many loci |	81343
             % of reads mapped to too many loci |	0.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.68%
                     % of reads unmapped: other |	0.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5435303	5435303	5435303
N_multimapping	1949274	1949274	1949274
N_noFeature	3257852	15777357	15387788
N_ambiguous	1692113	322825	332222
UnstrandedReadsAssigned:24021511 PositiveStrandReadsAssigned:12871294 NegativeStrandReadsAssigned:13251466
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=125 echo kmer=121
SRR14016380 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016380-trimmed-pair1.fastq
                             SRR14016380-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 36,356,053 reads, 27,441,794 reads pseudoaligned
[quant] estimated average fragment length: 177.747
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,150 rounds

 109283 SRR14016380.ke.tsv
  52636 SRR14016380.se.tsv
 161919 total
==> SRR14016380.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.33333	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	2	0	0
ENSMUST00000178537.1	12	2	0	0
ENSMUST00000178862.1	14	4	0	0
ENSMUST00000179520.1	11	2	0	0
ENSMUST00000179883.1	16	4.33333	0	0
ENSMUST00000195858.1	10	1	0	0
ENSMUST00000179932.1	12	2	0	0

==> SRR14016380.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR14016380 completed mapping pipeline successfully
