Starting /dee2/code/volunteer_pipeline.sh SRR14016357
    current disk space = 317012748
    free memory = 263474416 
SRR14016357 SRAfilesize
56565359575431062bca05f6cb396561  SRR14016357.sra
SRR14016357.sra file validated
SRR14016357 is paired end
SRR14016357 is conventional basespace
SRR14016357 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.31025	37.0	37.0	37.0	37.0	37.0
2	35.1685	37.0	37.0	37.0	25.0	37.0
3	35.3065	37.0	37.0	37.0	37.0	37.0
4	35.6055	37.0	37.0	37.0	37.0	37.0
5	35.576	37.0	37.0	37.0	37.0	37.0
6	35.4415	37.0	37.0	37.0	37.0	37.0
7	35.3435	37.0	37.0	37.0	37.0	37.0
8	35.4755	37.0	37.0	37.0	37.0	37.0
9	35.476	37.0	37.0	37.0	37.0	37.0
10-14	35.535199999999996	37.0	37.0	37.0	37.0	37.0
15-19	35.42900000000001	37.0	37.0	37.0	34.6	37.0
20-24	35.3622	37.0	37.0	37.0	34.6	37.0
25-29	35.1509	37.0	37.0	37.0	25.0	37.0
30-34	34.91609999999999	37.0	37.0	37.0	25.0	37.0
35-39	34.78965	37.0	37.0	37.0	25.0	37.0
40-44	34.7418	37.0	37.0	37.0	25.0	37.0
45-49	34.4551	37.0	37.0	37.0	25.0	37.0
50-54	34.0125	37.0	37.0	37.0	22.2	37.0
55-59	33.25575	37.0	37.0	37.0	13.8	37.0
60-64	32.7534	37.0	37.0	37.0	11.0	37.0
65-69	32.2355	37.0	37.0	37.0	11.0	37.0
70-74	31.7121	37.0	27.4	37.0	11.0	37.0
75-79	31.757349999999995	37.0	32.2	37.0	11.0	37.0
80-84	31.661949999999997	37.0	27.4	37.0	11.0	37.0
85-89	31.3473	37.0	25.0	37.0	11.0	37.0
90-94	31.006400000000003	37.0	25.0	37.0	11.0	37.0
95-99	30.62765	37.0	25.0	37.0	11.0	37.0
100-104	30.3265	37.0	25.0	37.0	11.0	37.0
105-109	29.918	37.0	25.0	37.0	11.0	37.0
110-114	29.59185	37.0	25.0	37.0	11.0	37.0
115-119	29.28095	37.0	22.2	37.0	11.0	37.0
120-124	28.8171	37.0	11.0	37.0	11.0	37.0
125-129	28.278699999999997	37.0	11.0	37.0	11.0	37.0
130-134	28.1207	37.0	11.0	37.0	11.0	37.0
135-139	27.803000000000004	37.0	11.0	37.0	11.0	37.0
140-144	27.78575	37.0	11.0	37.0	11.0	37.0
145-149	27.861849999999997	37.0	11.0	37.0	11.0	37.0
150	27.78325	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	2.0
20	3.0
21	22.0
22	45.0
23	125.0
24	160.0
25	233.0
26	235.0
27	223.0
28	177.0
29	143.0
30	129.0
31	167.0
32	156.0
33	215.0
34	466.0
35	587.0
36	855.0
37	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.188986232790988	24.430538172715895	12.565707133917398	31.81476846057572
2	15.856713426853709	25.551102204408814	29.458917835671343	29.13326653306613
3	22.725	22.0	32.324999999999996	22.95
4	19.725	35.949999999999996	17.125	27.200000000000003
5	21.725	41.575	17.724999999999998	18.975
6	26.900000000000002	26.6	27.075	19.425
7	21.875	23.549999999999997	26.724999999999998	27.85
8	26.224999999999998	31.474999999999998	20.175	22.125
9	24.275	22.725	32.025	20.974999999999998
10-14	21.93	28.83	24.43	24.81
15-19	24.505	26.790000000000003	23.49	25.215
20-24	25.3	27.12	27.794999999999998	19.785
25-29	20.674999999999997	26.85	28.04	24.435000000000002
30-34	23.134626925385078	26.195239047809558	30.57111422284457	20.09901980396079
35-39	18.957843676551484	25.80387058058709	35.11526729009351	20.123018452767916
40-44	21.48	23.515	36.08	18.925
45-49	19.685	25.040000000000003	36.99	18.285
50-54	20.82708270827083	26.632663266326634	34.10841084108411	18.43184318431843
55-59	22.681134056702835	26.151307565378268	31.071553577678884	20.09600480024001
60-64	22.5022502250225	29.137913791379138	28.487848784878487	19.87198719871987
65-69	22.509501900380076	30.426085217043408	27.71054210842168	19.35387077415483
70-74	22.969593918783758	31.486297259451888	25.93518703740748	19.608921784356873
75-79	23.795948987246813	32.168042010502624	23.495873968492123	20.54013503375844
80-84	23.93098274568642	31.442860715178796	23.925981495373843	20.70017504376094
85-89	22.6995399079816	33.27165433086618	21.099219843968793	22.929585917183438
90-94	22.241672501750525	33.555066519955986	21.311393418025407	22.891867560268082
95-99	20.635158789697424	35.58389597399349	20.29007251812953	23.490872718179546
100-104	18.740622186655997	37.22116634990497	20.256076823046914	23.782134640392115
105-109	19.23884776955391	36.6873374674935	20.979195839167833	23.094618923784758
110-114	18.4896224056014	37.4593648412103	21.010252563140785	23.040760190047514
115-119	20.205051262815704	35.57389347336834	21.660415103775943	22.56064016004001
120-124	21.359271854370874	36.03220644128825	21.43428685737147	21.174234846969394
125-129	23.309661932386476	35.46709341868374	20.629125825165033	20.594118823764752
130-134	25.695139027805563	35.26705341068214	20.08901780356071	18.94878975795159
135-139	28.297829782978294	32.53825382538254	19.726972697269726	19.43694369436944
140-144	31.819772965944892	32.39485922888433	19.242886432964944	16.54248137220583
145-149	36.48412103025757	30.622655663915978	18.42960740185046	14.463615903975994
150	39.98499624906226	28.157039259814955	17.129282320580145	14.728682170542637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	2.0
4	2.0
5	1.5
6	0.5
7	3.5
8	4.0
9	4.5
10	6.5
11	4.5
12	7.0
13	14.0
14	14.5
15	15.0
16	18.0
17	23.0
18	32.0
19	31.0
20	25.5
21	33.5
22	41.0
23	39.5
24	37.0
25	35.5
26	45.0
27	55.0
28	55.5
29	56.5
30	64.0
31	68.0
32	69.5
33	81.5
34	91.0
35	97.5
36	92.5
37	83.0
38	80.0
39	64.5
40	44.5
41	45.0
42	49.0
43	50.0
44	58.5
45	72.0
46	97.5
47	124.5
48	126.5
49	134.5
50	156.0
51	173.0
52	177.0
53	186.0
54	182.5
55	163.5
56	139.0
57	114.5
58	105.5
59	105.5
60	88.0
61	51.0
62	36.5
63	27.5
64	26.0
65	27.0
66	20.0
67	8.5
68	3.0
69	1.0
70	0.5
71	0.0
72	0.0
73	1.5
74	1.5
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.2
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.02
35-39	0.015
40-44	0.0
45-49	0.0
50-54	0.01
55-59	0.005
60-64	0.01
65-69	0.02
70-74	0.02
75-79	0.025
80-84	0.025
85-89	0.02
90-94	0.03
95-99	0.025
100-104	0.03
105-109	0.02
110-114	0.025
115-119	0.025
120-124	0.02
125-129	0.02
130-134	0.02
135-139	0.01
140-144	0.015
145-149	0.025
150	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45762711864407	45.7
2	12.584745762711863	14.85
3	4.61864406779661	8.175
4	1.9915254237288134	4.7
5	1.1864406779661016	3.5000000000000004
6	0.6355932203389831	2.25
7	0.5932203389830508	2.45
8	0.1694915254237288	0.8
9	0.2542372881355932	1.35
>10	0.38135593220338987	3.175
>50	0.0423728813559322	1.6500000000000001
>100	0.0847457627118644	11.4
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	231	5.775	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	225	5.625	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	66	1.6500000000000001	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.675	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.475	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	16	0.4	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	12	0.3	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	12	0.3	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	11	0.27499999999999997	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	10	0.25	No Hit
CTTCTTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATC	10	0.25	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	10	0.25	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	9	0.22499999999999998	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	9	0.22499999999999998	No Hit
ATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGCC	9	0.22499999999999998	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	9	0.22499999999999998	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	9	0.22499999999999998	No Hit
ATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	8	0.2	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	8	0.2	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	7	0.17500000000000002	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	7	0.17500000000000002	No Hit
CTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGG	7	0.17500000000000002	No Hit
GCCAGAGGCTGGTGATGGGACCACCGAGATCTCACCCACTGGTGCTGCTG	7	0.17500000000000002	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	7	0.17500000000000002	No Hit
CTCACGGTAGGCCCGCTTCTCCACCAGCTTCACTTTGTATTTGCGCTTGA	7	0.17500000000000002	No Hit
TGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAA	7	0.17500000000000002	No Hit
CTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGA	7	0.17500000000000002	No Hit
CTCAGGACACTGAAGCCAGAAGGTCACATTCTCCGAGACAGGTTCAAGAG	7	0.17500000000000002	No Hit
GTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCA	7	0.17500000000000002	No Hit
TTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACG	7	0.17500000000000002	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	6	0.15	No Hit
CCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGATACCACTGC	6	0.15	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	6	0.15	No Hit
GTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGCTCTTGAACCTGTCT	6	0.15	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTAA	6	0.15	No Hit
CTTGAACTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGC	6	0.15	No Hit
CAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAG	6	0.15	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	6	0.15	No Hit
GGTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACG	6	0.15	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	6	0.15	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	6	0.15	No Hit
ATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTG	6	0.15	No Hit
TCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CAAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAACCA	5	0.125	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	5	0.125	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	5	0.125	No Hit
CTTACGCAAAGGTCCTAGAAGACAACTCCGTACCTCAGGTCAAAGACATC	5	0.125	No Hit
GTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTCCTGGGGCC	5	0.125	No Hit
GAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTCC	5	0.125	No Hit
CAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGC	5	0.125	No Hit
TCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCT	5	0.125	No Hit
GAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCC	5	0.125	No Hit
CTGCAGGAATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGG	5	0.125	No Hit
CCCAAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAAC	5	0.125	No Hit
GAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCC	5	0.125	No Hit
TTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCG	5	0.125	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	5	0.125	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	5	0.125	No Hit
CCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAAAAAAAA	5	0.125	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	5	0.125	No Hit
CGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAA	5	0.125	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	5	0.125	No Hit
TCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGA	5	0.125	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	5	0.125	No Hit
CTGCAGTGGAGGTGATTCCTGCAGGAGCCTCCTACAACCCAACCTTTGAA	5	0.125	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTC	5	0.125	No Hit
TTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTG	5	0.125	No Hit
AGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGT	5	0.125	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0125	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.05	0.0	0.0	0.0	0.0
30-31	0.2875	0.0	0.0	0.0	0.0
32-33	0.4875	0.0	0.0	0.0	0.0
34-35	0.7125	0.0	0.0	0.0	0.0
36-37	1.0375	0.0	0.0	0.0	0.0
38-39	1.2	0.0	0.0	0.0	0.0
40-41	1.5125000000000002	0.0	0.0	0.0	0.0
42-43	1.9375	0.0	0.0	0.0	0.0
44-45	2.2125000000000004	0.0	0.0	0.0	0.0
46-47	2.75	0.0	0.0	0.0	0.0
48-49	3.1624999999999996	0.0	0.0	0.0	0.0
50-51	3.575	0.0	0.0	0.0	0.0
52-53	4.1625	0.0	0.0	0.0	0.0
54-55	5.1875	0.0	0.0	0.0	0.0
56-57	6.075	0.0	0.0	0.0	0.0
58-59	6.9	0.0	0.0	0.0	0.0
60-61	8.1125	0.0	0.0	0.0	0.0
62-63	9.375	0.0	0.0	0.0	0.0
64-65	10.775	0.0	0.0	0.0	0.0
66-67	12.35	0.0	0.0	0.0	0.0
68-69	13.775	0.0	0.0	0.0	0.0
70-71	14.9	0.0	0.0	0.0	0.0
72-73	16.2125	0.0	0.0	0.0	0.0
74-75	18.325	0.0	0.0	0.0	0.0
76-77	19.762500000000003	0.0	0.0	0.0	0.0
78-79	21.4625	0.0	0.0	0.0	0.0
80-81	23.05	0.0	0.0	0.0	0.0
82-83	24.6875	0.0	0.0	0.0	0.0
84-85	26.2875	0.0	0.0	0.0	0.0
86-87	28.1	0.0	0.0	0.0	0.0
88-89	29.7	0.0	0.0	0.0	0.0
90-91	31.1625	0.0	0.0	0.0	0.0
92-93	32.9625	0.0	0.0	0.0	0.0
94-95	34.525000000000006	0.0	0.0	0.0	0.0
96-97	35.7375	0.0	0.0	0.0	0.0
98-99	37.65	0.0	0.0	0.0	0.0
100-101	38.5875	0.0	0.0	0.0	0.0
102-103	39.2	0.0	0.0	0.0	0.0
104-105	39.8375	0.0	0.0	0.0	0.0
106-107	40.475	0.0	0.0	0.0	0.0
108-109	41.150000000000006	0.0	0.0	0.0	0.0
110-111	41.662499999999994	0.0	0.0	0.0	0.0
112-113	42.4	0.0	0.0	0.0	0.0
114-115	42.837500000000006	0.0	0.0	0.0	0.0
116-117	43.1125	0.0	0.0	0.0	0.0
118-119	43.55	0.0	0.0	0.0	0.0
120-121	44.175	0.0	0.0	0.0	0.0
122-123	44.675	0.0	0.0	0.0	0.0
124-125	45.125	0.0	0.0	0.0	0.0
126-127	45.6125	0.0	0.0	0.0	0.0
128-129	46.0	0.0	0.0	0.0	0.0
130-131	46.425	0.0	0.0	0.0	0.0
132-133	46.975	0.0	0.0	0.0	0.0
134-135	47.2375	0.0	0.0	0.0	0.0
136-137	47.650000000000006	0.0	0.0	0.0	0.0
138	47.95	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	40	0.0	144.0	1
CTAATCA	40	0.0	144.0	2
CAGACCA	10	0.006973645	144.0	4
TTGCAAG	45	0.0	128.0	9
CATTGCA	45	0.0	128.0	7
AATCATT	45	0.0	128.0	4
TCATTGC	45	0.0	128.0	6
ATTGCAA	50	1.8189894E-12	115.2	8
TAATCAT	50	1.8189894E-12	115.2	3
ATCATTG	55	1.8189894E-12	104.72727	5
AAGCAGT	65	9.4921234E-7	44.30769	1
TGGTATC	75	3.7340433E-6	38.399998	7
AGTGGTA	75	3.7340433E-6	38.399998	5
GTATCAA	75	7.361034E-5	38.399998	9
AGCAGTG	75	3.7340433E-6	38.399998	2
GCAGTGG	75	7.361034E-5	38.399998	3
GTGGTAT	75	3.7340433E-6	38.399998	6
CAGTGGT	75	7.361034E-5	38.399998	4
GGTATCA	75	7.361034E-5	38.399998	8
GTACATG	20	0.006139246	28.8	30-34
>>END_MODULE
SRR14016357 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016357_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.23625	37.0	37.0	37.0	25.0	37.0
2	34.08825	37.0	37.0	37.0	25.0	37.0
3	34.403	37.0	37.0	37.0	25.0	37.0
4	34.7635	37.0	37.0	37.0	25.0	37.0
5	34.93	37.0	37.0	37.0	25.0	37.0
6	34.8795	37.0	37.0	37.0	25.0	37.0
7	34.7035	37.0	37.0	37.0	25.0	37.0
8	34.9635	37.0	37.0	37.0	25.0	37.0
9	34.821	37.0	37.0	37.0	25.0	37.0
10-14	34.867900000000006	37.0	37.0	37.0	25.0	37.0
15-19	34.8289	37.0	37.0	37.0	25.0	37.0
20-24	34.7862	37.0	37.0	37.0	25.0	37.0
25-29	34.63250000000001	37.0	37.0	37.0	25.0	37.0
30-34	34.5904	37.0	37.0	37.0	25.0	37.0
35-39	34.4071	37.0	37.0	37.0	25.0	37.0
40-44	34.2991	37.0	37.0	37.0	25.0	37.0
45-49	34.0086	37.0	37.0	37.0	25.0	37.0
50-54	33.6169	37.0	37.0	37.0	16.6	37.0
55-59	32.7271	37.0	37.0	37.0	11.0	37.0
60-64	32.4474	37.0	37.0	37.0	11.0	37.0
65-69	31.616899999999998	37.0	29.8	37.0	11.0	37.0
70-74	31.159999999999997	37.0	25.0	37.0	11.0	37.0
75-79	31.1852	37.0	25.0	37.0	11.0	37.0
80-84	30.970000000000006	37.0	25.0	37.0	11.0	37.0
85-89	30.580000000000002	37.0	25.0	37.0	11.0	37.0
90-94	30.485400000000006	37.0	25.0	37.0	11.0	37.0
95-99	29.950799999999997	37.0	25.0	37.0	11.0	37.0
100-104	29.907599999999995	37.0	25.0	37.0	11.0	37.0
105-109	29.6118	37.0	25.0	37.0	11.0	37.0
110-114	29.383300000000002	37.0	25.0	37.0	11.0	37.0
115-119	29.194100000000002	37.0	19.4	37.0	11.0	37.0
120-124	29.006899999999995	37.0	16.6	37.0	11.0	37.0
125-129	28.5491	37.0	11.0	37.0	11.0	37.0
130-134	28.3838	37.0	11.0	37.0	11.0	37.0
135-139	28.0589	37.0	11.0	37.0	11.0	37.0
140-144	28.012300000000003	37.0	11.0	37.0	11.0	37.0
145-149	27.941899999999997	37.0	11.0	37.0	11.0	37.0
150	27.9435	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	7.0
20	20.0
21	43.0
22	86.0
23	143.0
24	222.0
25	256.0
26	245.0
27	183.0
28	126.0
29	97.0
30	134.0
31	136.0
32	149.0
33	247.0
34	416.0
35	643.0
36	810.0
37	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.457614403600903	23.10577644411103	12.478119529882472	33.9584896224056
2	15.303825956489122	25.756439109777446	30.43260815203801	28.507126781695426
3	20.724999999999998	22.625	31.85	24.8
4	19.35	35.825	18.725	26.1
5	21.75	41.349999999999994	18.175	18.725
6	26.05	28.199999999999996	27.700000000000003	18.05
7	20.724999999999998	23.474999999999998	26.775	29.025000000000002
8	24.375	32.425	20.7	22.5
9	22.55	21.925	32.85	22.675
10-14	22.495	29.225	23.73	24.55
15-19	25.019999999999996	25.825	23.880000000000003	25.275
20-24	25.555	27.0	26.965	20.48
25-29	20.605	27.565	27.04	24.79
30-34	23.44	26.8	28.74	21.02
35-39	18.965	25.995	34.294999999999995	20.745
40-44	20.695	24.795	35.485	19.025
45-49	20.06	25.655	36.19	18.095
50-54	20.794999999999998	25.765	34.67	18.77
55-59	23.015	27.084999999999997	31.730000000000004	18.17
60-64	23.175	29.220000000000002	29.385	18.22
65-69	24.69	29.7	28.444999999999997	17.165
70-74	25.955000000000002	30.78	25.845000000000002	17.419999999999998
75-79	28.249999999999996	30.775000000000002	23.849999999999998	17.125
80-84	28.749999999999996	30.395	24.385	16.470000000000002
85-89	29.349999999999998	32.2	21.91	16.54
90-94	30.0	32.23	21.81	15.959999999999999
95-99	29.909999999999997	33.489999999999995	21.115000000000002	15.485
100-104	29.770000000000003	34.405	20.645	15.18
105-109	30.485	33.815	21.57	14.13
110-114	30.305	34.44	20.9	14.355
115-119	30.91	34.77	21.325	12.995000000000001
120-124	31.879999999999995	34.4	20.605	13.114999999999998
125-129	33.08	34.18	19.91	12.83
130-134	36.725	32.535	18.970000000000002	11.77
135-139	39.050000000000004	31.295	17.66	11.995000000000001
140-144	42.76	30.735	16.705000000000002	9.8
145-149	45.695	29.595	15.425	9.285
150	48.3	27.650000000000002	15.8	8.25
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	1.5
5	1.0
6	0.5
7	1.0
8	3.0
9	4.5
10	3.5
11	4.0
12	8.0
13	11.5
14	14.5
15	17.0
16	17.0
17	22.5
18	30.5
19	32.5
20	38.0
21	37.0
22	35.0
23	40.0
24	43.0
25	50.0
26	53.0
27	52.0
28	51.5
29	60.5
30	70.0
31	68.0
32	66.0
33	76.0
34	85.5
35	84.0
36	77.5
37	68.0
38	62.5
39	58.5
40	53.5
41	54.0
42	58.0
43	57.0
44	60.5
45	72.0
46	87.0
47	103.5
48	115.5
49	134.5
50	156.0
51	178.5
52	187.0
53	199.0
54	178.5
55	142.5
56	135.5
57	122.5
58	114.0
59	109.5
60	95.0
61	64.0
62	40.5
63	28.0
64	29.0
65	29.5
66	19.5
67	7.5
68	1.5
69	1.5
70	2.0
71	1.5
72	0.5
73	0.5
74	2.5
75	2.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22920892494929	48.825
2	11.27789046653144	13.900000000000002
3	4.056795131845842	7.5
4	2.636916835699797	6.5
5	0.8113590263691683	2.5
6	0.486815415821501	1.7999999999999998
7	0.5273833671399595	2.275
8	0.12170385395537525	0.6
9	0.2434077079107505	1.35
>10	0.5273833671399595	4.9750000000000005
>50	0.0	0.0
>100	0.08113590263691683	9.775
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	236	5.8999999999999995	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	155	3.875	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	49	1.225	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.6	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	17	0.42500000000000004	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	13	0.325	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.3	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	12	0.3	No Hit
ATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCTC	11	0.27499999999999997	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	11	0.27499999999999997	No Hit
CTCAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTC	10	0.25	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	10	0.25	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	10	0.25	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	10	0.25	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	9	0.22499999999999998	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	9	0.22499999999999998	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
GTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAA	9	0.22499999999999998	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	9	0.22499999999999998	No Hit
CATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCT	8	0.2	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	8	0.2	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	8	0.2	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	7	0.17500000000000002	No Hit
GTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGA	7	0.17500000000000002	No Hit
GAATCATTCTAAGATCCCTGCTCCCAAAGACATCCTCGCACATCAGGTCC	7	0.17500000000000002	No Hit
CGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCA	7	0.17500000000000002	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	7	0.17500000000000002	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	7	0.17500000000000002	No Hit
CCCAAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAAC	7	0.17500000000000002	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGC	7	0.17500000000000002	No Hit
CAAGAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCC	7	0.17500000000000002	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	7	0.17500000000000002	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	7	0.17500000000000002	No Hit
GGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCC	6	0.15	No Hit
CTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGG	6	0.15	No Hit
TGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAG	6	0.15	No Hit
ATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTG	6	0.15	No Hit
CATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGC	6	0.15	No Hit
GTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGA	6	0.15	No Hit
AATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTG	6	0.15	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	6	0.15	No Hit
GGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGCCTTTCTTCTTGGTCTG	6	0.15	No Hit
CTCAGAGCTGAGCTGCACATCAATGTCAGGAGCCTGGTACTTGAGCCGTC	6	0.15	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	6	0.15	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	6	0.15	No Hit
GTTGGCATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAA	5	0.125	No Hit
CCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.125	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	5	0.125	No Hit
CCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTC	5	0.125	No Hit
GAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCC	5	0.125	No Hit
GTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCA	5	0.125	No Hit
CTCTTCTTTCTTGGTTCTTTTCTCTTGAATCCTGTGTCCACGAAGAAGAG	5	0.125	No Hit
CAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCAGGC	5	0.125	No Hit
CATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAACAATTTAAT	5	0.125	No Hit
GAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAG	5	0.125	No Hit
CTGCCTCTTTCTCACGCTGCAGCTCCACCTCATGGGCCTCTCGAAGCAGG	5	0.125	No Hit
CGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGC	5	0.125	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	5	0.125	No Hit
CCACCTCATGGGCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGG	5	0.125	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	5	0.125	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	5	0.125	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	5	0.125	No Hit
CTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAA	5	0.125	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0125	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.05	0.0	0.0	0.0	0.0
30-31	0.2875	0.0	0.0	0.0	0.0
32-33	0.5125	0.0	0.0	0.0	0.0
34-35	0.7250000000000001	0.0	0.0	0.0	0.0
36-37	1.0375	0.0	0.0	0.0	0.0
38-39	1.2	0.0	0.0	0.0	0.0
40-41	1.4874999999999998	0.0	0.0	0.0	0.0
42-43	1.9375	0.0	0.0	0.0	0.0
44-45	2.2625	0.0	0.0	0.0	0.0
46-47	2.8	0.0	0.0	0.0	0.0
48-49	3.2375	0.0	0.0	0.0	0.0
50-51	3.6500000000000004	0.0	0.0	0.0	0.0
52-53	4.2125	0.0	0.0	0.0	0.0
54-55	5.225	0.0	0.0	0.0	0.0
56-57	6.125	0.0	0.0	0.0	0.0
58-59	6.95	0.0	0.0	0.0	0.0
60-61	8.1375	0.0	0.0	0.0	0.0
62-63	9.4125	0.0	0.0	0.0	0.0
64-65	10.825	0.0	0.0	0.0	0.0
66-67	12.4375	0.0	0.0	0.0	0.0
68-69	13.8625	0.0	0.0	0.0	0.0
70-71	14.975	0.0	0.0	0.0	0.0
72-73	16.325	0.0	0.0	0.0	0.0
74-75	18.3875	0.0	0.0	0.0	0.0
76-77	19.8125	0.0	0.0	0.0	0.0
78-79	21.5	0.0	0.0	0.0	0.0
80-81	23.1	0.0	0.0	0.0	0.0
82-83	24.7	0.0	0.0	0.0	0.0
84-85	26.362499999999997	0.0	0.0	0.0	0.0
86-87	28.1	0.0	0.0	0.0	0.0
88-89	29.5875	0.0	0.0	0.0	0.0
90-91	30.9125	0.0	0.0	0.0	0.0
92-93	32.599999999999994	0.0	0.0	0.0	0.0
94-95	34.162499999999994	0.0	0.0	0.0	0.0
96-97	35.287499999999994	0.0	0.0	0.0	0.0
98-99	37.225	0.0	0.0	0.0	0.0
100-101	38.150000000000006	0.0	0.0	0.0	0.0
102-103	38.775	0.0	0.0	0.0	0.0
104-105	39.474999999999994	0.0	0.0	0.0	0.0
106-107	40.0875	0.0	0.0	0.0	0.0
108-109	40.8	0.0	0.0	0.0	0.0
110-111	41.3375	0.0	0.0	0.0	0.0
112-113	42.1	0.0	0.0	0.0	0.0
114-115	42.525	0.0	0.0	0.0	0.0
116-117	42.7625	0.0	0.0	0.0	0.0
118-119	43.212500000000006	0.0	0.0	0.0	0.0
120-121	43.7625	0.0	0.0	0.0	0.0
122-123	44.2375	0.0	0.0	0.0	0.0
124-125	44.7375	0.0	0.0	0.0	0.0
126-127	45.287499999999994	0.0	0.0	0.0	0.0
128-129	45.6875	0.0	0.0	0.0	0.0
130-131	46.1125	0.0	0.0	0.0	0.0
132-133	46.6375	0.0	0.0	0.0	0.0
134-135	46.8625	0.0	0.0	0.0	0.0
136-137	47.25	0.0	0.0	0.0	0.0
138	47.575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	25	3.201967E-8	144.0	1
CTAATCA	35	2.3845314E-7	102.85714	2
TTGCAAG	40	5.2840005E-7	90.0	9
CATTGCA	40	5.2840005E-7	90.0	7
TAATCAT	40	5.2840005E-7	90.0	3
TCATTGC	40	5.2840005E-7	90.0	6
AATCATT	45	1.0653384E-6	80.0	4
ATTGCAA	45	1.0653384E-6	80.0	8
ATCATTG	45	1.0653384E-6	80.0	5
AGTGGTA	55	5.0997805E-6	39.272728	5
CAGTGGT	55	0.0026350126	39.272728	4
AAGCAGT	55	5.0997805E-6	39.272728	1
TGGTATC	60	1.0824771E-5	36.0	7
GCAAGCA	40	3.1003024E-4	36.0	9
GTATCAA	60	0.0047032754	36.0	9
GCAGTGG	80	0.001866456	36.0	3
GTGGTAT	60	1.0824771E-5	36.0	6
AGCAGTG	65	2.1579981E-5	33.230766	2
CAAGCAG	40	3.180794E-7	28.8	10-14
TCAACGC	70	4.0784E-5	20.571428	2
>>END_MODULE
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:44:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:45:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:45:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:46:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
2023-05-11T07:46:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454887 spots for SRR14016357.sra
Written 454887 spots for SRR14016357.sra
Read 454919 spots for SRR14016357.sra
Written 454919 spots for SRR14016357.sra
SRR ids: ['SRR14016357.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hly3xnd1
SRR14016357.sra spots: 29112800
blocks: [[1, 454887], [454888, 909774], [909775, 1364661], [1364662, 1819548], [1819549, 2274435], [2274436, 2729322], [2729323, 3184209], [3184210, 3639096], [3639097, 4093983], [4093984, 4548870], [4548871, 5003757], [5003758, 5458644], [5458645, 5913531], [5913532, 6368418], [6368419, 6823305], [6823306, 7278192], [7278193, 7733079], [7733080, 8187966], [8187967, 8642853], [8642854, 9097740], [9097741, 9552627], [9552628, 10007514], [10007515, 10462401], [10462402, 10917288], [10917289, 11372175], [11372176, 11827062], [11827063, 12281949], [12281950, 12736836], [12736837, 13191723], [13191724, 13646610], [13646611, 14101497], [14101498, 14556384], [14556385, 15011271], [15011272, 15466158], [15466159, 15921045], [15921046, 16375932], [16375933, 16830819], [16830820, 17285706], [17285707, 17740593], [17740594, 18195480], [18195481, 18650367], [18650368, 19105254], [19105255, 19560141], [19560142, 20015028], [20015029, 20469915], [20469916, 20924802], [20924803, 21379689], [21379690, 21834576], [21834577, 22289463], [22289464, 22744350], [22744351, 23199237], [23199238, 23654124], [23654125, 24109011], [24109012, 24563898], [24563899, 25018785], [25018786, 25473672], [25473673, 25928559], [25928560, 26383446], [26383447, 26838333], [26838334, 27293220], [27293221, 27748107], [27748108, 28202994], [28202995, 28657881], [28657882, 29112800]]
SRR14016357 file size 9815248
SRR14016357 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016357 SRR14016357_1.fastq SRR14016357_2.fastq
Input file:	SRR14016357_1.fastq
Paired file:	SRR14016357_2.fastq
trimmed:	SRR14016357-trimmed-pair1.fastq, SRR14016357-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 07:46:48 2023 >> started

Thu May 11 07:47:53 2023 >> done (64.594s)
29112800 read pairs processed; of these:
    6147 ( 0.02%) short read pairs filtered out after trimming by size control
   10380 ( 0.04%) empty read pairs filtered out after trimming by size control
29096273 (99.94%) read pairs available; of these:
13085660 (44.97%) trimmed read pairs available after processing
16010613 (55.03%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     496	  0.00%
 19	     394	  0.00%
 20	     868	  0.00%
 21	    1342	  0.00%
 22	    3329	  0.01%
 23	    2729	  0.01%
 24	    2982	  0.01%
 25	    4078	  0.01%
 26	    6099	  0.02%
 27	    9154	  0.03%
 28	   14408	  0.05%
 29	   20547	  0.07%
 30	   27462	  0.09%
 31	   38266	  0.13%
 32	   49279	  0.17%
 33	   46509	  0.16%
 34	   54729	  0.19%
 35	   51055	  0.18%
 36	   49689	  0.17%
 37	   55434	  0.19%
 38	   57792	  0.20%
 39	   65515	  0.23%
 40	   65824	  0.23%
 41	   70106	  0.24%
 42	   85750	  0.29%
 43	   77721	  0.27%
 44	   81022	  0.28%
 45	   81726	  0.28%
 46	   83865	  0.29%
 47	   87121	  0.30%
 48	   90883	  0.31%
 49	   94655	  0.33%
 50	   98227	  0.34%
 51	  101519	  0.35%
 52	  105852	  0.36%
 53	  110170	  0.38%
 54	  112577	  0.39%
 55	  114559	  0.39%
 56	  115000	  0.40%
 57	  116070	  0.40%
 58	  117757	  0.40%
 59	  118474	  0.41%
 60	  119360	  0.41%
 61	  121095	  0.42%
 62	  122190	  0.42%
 63	  124209	  0.43%
 64	  125975	  0.43%
 65	  126966	  0.44%
 66	  127468	  0.44%
 67	  128452	  0.44%
 68	  128360	  0.44%
 69	  128953	  0.44%
 70	  129575	  0.45%
 71	  130212	  0.45%
 72	  131168	  0.45%
 73	  132143	  0.45%
 74	  133116	  0.46%
 75	  133057	  0.46%
 76	  133952	  0.46%
 77	  132199	  0.45%
 78	  131005	  0.45%
 79	  132218	  0.45%
 80	  131480	  0.45%
 81	  130798	  0.45%
 82	  131491	  0.45%
 83	  131519	  0.45%
 84	  131793	  0.45%
 85	  131635	  0.45%
 86	  130648	  0.45%
 87	  129402	  0.44%
 88	  129228	  0.44%
 89	  128606	  0.44%
 90	  128125	  0.44%
 91	  127595	  0.44%
 92	  126947	  0.44%
 93	  126779	  0.44%
 94	  127842	  0.44%
 95	  126635	  0.44%
 96	  126624	  0.44%
 97	  125265	  0.43%
 98	  124909	  0.43%
 99	  123976	  0.43%
100	  123199	  0.42%
101	  122352	  0.42%
102	  122191	  0.42%
103	  122370	  0.42%
104	  121936	  0.42%
105	  121003	  0.42%
106	  121207	  0.42%
107	  119722	  0.41%
108	  118252	  0.41%
109	  118433	  0.41%
110	  117165	  0.40%
111	  116595	  0.40%
112	  116405	  0.40%
113	  115828	  0.40%
114	  115183	  0.40%
115	  115233	  0.40%
116	  114660	  0.39%
117	  113213	  0.39%
118	  112878	  0.39%
119	  111782	  0.38%
120	  110614	  0.38%
121	  111322	  0.38%
122	  110293	  0.38%
123	  109413	  0.38%
124	  109806	  0.38%
125	  108625	  0.37%
126	  108322	  0.37%
127	  108193	  0.37%
128	  106237	  0.37%
129	  105877	  0.36%
130	  105313	  0.36%
131	  104868	  0.36%
132	  104178	  0.36%
133	  103443	  0.36%
134	  103769	  0.36%
135	  102223	  0.35%
136	  102564	  0.35%
137	  101812	  0.35%
138	  101182	  0.35%
139	  100005	  0.34%
140	   98348	  0.34%
141	   98851	  0.34%
142	   98460	  0.34%
143	   98237	  0.34%
144	   96353	  0.33%
145	   96306	  0.33%
146	   95924	  0.33%
147	   95924	  0.33%
148	   95632	  0.33%
149	   99955	  0.34%
150	16010613	 55.03%
29096273 reads passed initial QC


criterion=sequence-density
sequence-density=4.95
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=42
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=82.31
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=7.1
sequence=CCTCCTGCTCCCCATTTTCCTCATTTTGAGCGTTCCCATTGGCAGGTGCATCTCTTCCATTCTCTGCCTCCTCCACAACTTCCTTCTTCTCCTTCAAGTCCTTGGTGGTGATCTCGGAGCTGGTATCCACTGCCGCGTCTGACATGGTGGGGCACGCCGGTGGTCCGATGCCGCGAGTGAGGGAATTAAAAGTCGAGTTCGAGGACTCTGGCGAGAGAGCTGCCGGAGTCCGCGCTGGAGGAGGCGGTGGGCGGCGAAGGTGGCTGCAGCGAGCGCGGAGCACGACCCAGGAACAATGCAAAGATGGCTTTTCGGAGCAGCCAGTGGGGCCCCATG


criterion=sequence-density
sequence-density=1.38
sequence-density-rank=1
fanout-score=28.73
fanout-score-rank=5
prefix-density=1.40
prefix-fanout=28.4
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA


criterion=fanout-score
sequence-density=0.13
sequence-density-rank=29
fanout-score=69.26
fanout-score-rank=1
prefix-density=8.96
prefix-fanout=1.0
sequence=ACGCAGAGTACT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG -y CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA -o SRR14016357 SRR14016357_1.fastq SRR14016357_2.fastq
Input file:	SRR14016357_1.fastq
Paired file:	SRR14016357_2.fastq
trimmed:	SRR14016357-trimmed-pair1.fastq, SRR14016357-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG
-- paired 3' end adapter sequence (-y):	CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 07:51:01 2023 >> started

Thu May 11 07:51:31 2023 >> done (29.356s)
14548137 read pairs processed; of these:
     158 ( 0.00%) short read pairs filtered out after trimming by size control
  496465 ( 3.41%) empty read pairs filtered out after trimming by size control
14051514 (96.59%) read pairs available; of these:
     505 ( 0.00%) trimmed read pairs available after processing
14051009 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     206	  0.00%
 19	     194	  0.00%
 20	     416	  0.00%
 21	     580	  0.00%
 22	    1624	  0.01%
 23	    1382	  0.01%
 24	    1508	  0.01%
 25	    1958	  0.01%
 26	    2993	  0.02%
 27	    4587	  0.03%
 28	    7098	  0.05%
 29	   10209	  0.07%
 30	   13529	  0.10%
 31	   18854	  0.13%
 32	   23630	  0.17%
 33	   22787	  0.16%
 34	   24175	  0.17%
 35	   23925	  0.17%
 36	   24639	  0.18%
 37	   26460	  0.19%
 38	   28484	  0.20%
 39	   30627	  0.22%
 40	   32433	  0.23%
 41	   34167	  0.24%
 42	   39145	  0.28%
 43	   37885	  0.27%
 44	   39723	  0.28%
 45	   40579	  0.29%
 46	   41706	  0.30%
 47	   43200	  0.31%
 48	   44749	  0.32%
 49	   46704	  0.33%
 50	   48695	  0.35%
 51	   49863	  0.35%
 52	   52364	  0.37%
 53	   53577	  0.38%
 54	   54791	  0.39%
 55	   56053	  0.40%
 56	   55898	  0.40%
 57	   56945	  0.41%
 58	   57637	  0.41%
 59	   57642	  0.41%
 60	   58809	  0.42%
 61	   59291	  0.42%
 62	   59779	  0.43%
 63	   60640	  0.43%
 64	   61746	  0.44%
 65	   62535	  0.45%
 66	   62135	  0.44%
 67	   62747	  0.45%
 68	   63024	  0.45%
 69	   62761	  0.45%
 70	   62735	  0.45%
 71	   63603	  0.45%
 72	   64209	  0.46%
 73	   64524	  0.46%
 74	   64918	  0.46%
 75	   65081	  0.46%
 76	   65058	  0.46%
 77	   64624	  0.46%
 78	   64035	  0.46%
 79	   64486	  0.46%
 80	   64006	  0.46%
 81	   63936	  0.46%
 82	   64408	  0.46%
 83	   64062	  0.46%
 84	   64421	  0.46%
 85	   64845	  0.46%
 86	   63476	  0.45%
 87	   63307	  0.45%
 88	   63400	  0.45%
 89	   62192	  0.44%
 90	   61978	  0.44%
 91	   61231	  0.44%
 92	   61864	  0.44%
 93	   62242	  0.44%
 94	   62251	  0.44%
 95	   61492	  0.44%
 96	   61433	  0.44%
 97	   60747	  0.43%
 98	   60657	  0.43%
 99	   59644	  0.42%
100	   59812	  0.43%
101	   59434	  0.42%
102	   59649	  0.42%
103	   59857	  0.43%
104	   59262	  0.42%
105	   58067	  0.41%
106	   58295	  0.41%
107	   57608	  0.41%
108	   57379	  0.41%
109	   57636	  0.41%
110	   56784	  0.40%
111	   56984	  0.41%
112	   56764	  0.40%
113	   55869	  0.40%
114	   55459	  0.39%
115	   55645	  0.40%
116	   55736	  0.40%
117	   55128	  0.39%
118	   54886	  0.39%
119	   54825	  0.39%
120	   53812	  0.38%
121	   53972	  0.38%
122	   53779	  0.38%
123	   53115	  0.38%
124	   52976	  0.38%
125	   52658	  0.37%
126	   51865	  0.37%
127	   52573	  0.37%
128	   51579	  0.37%
129	   51315	  0.37%
130	   51133	  0.36%
131	   50828	  0.36%
132	   50869	  0.36%
133	   49652	  0.35%
134	   49995	  0.36%
135	   49290	  0.35%
136	   49227	  0.35%
137	   49105	  0.35%
138	   48667	  0.35%
139	   48360	  0.34%
140	   47521	  0.34%
141	   46966	  0.33%
142	   47643	  0.34%
143	   47308	  0.34%
144	   46229	  0.33%
145	   46502	  0.33%
146	   46011	  0.33%
147	   46122	  0.33%
148	   46665	  0.33%
149	   48205	  0.34%
150	 7690540	 54.73%


criterion=sequence-density
sequence-density=1.72
sequence-density-rank=1
fanout-score=27.18
fanout-score-rank=4
prefix-density=1.74
prefix-fanout=27.0
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCAGATCGGAAG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=30
fanout-score=39.80
fanout-score-rank=1
prefix-density=0.36
prefix-fanout=3.2
sequence=GCAGCAGCGCCTGTGGGAAAAACTAGAGGCTAGAGCCATGGATGACCAACGCGACCTCATCTCTAACCATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGCCAGAAAGGTGCAGCCGTGGAGCTCTGTACACCGGTGTCTCTGTCCTGGTGGCTCTGCTCTTGGCTGGGCAGGCCACCACTGCTTACTTCCTGTACCAGCAACAGGGCCGCCTAGACAAGCTGACCATCACCTCCCAGAACCTGCAACTGGAGAGCCTTCGCATGAAGCTTCCGAAATCTGCCAAACCTGTGAGCCAGATGCGGATGGCTACTCCCTTGCTGATGCGTCCAATGTCCATGGATAACATGCTCCTTGGGCCTGTGAAGAACGTTACCAAGTACGGCAACATGACCCAGGACCATGTGATGCATCTGCTCACGAGGTCTGGACCCCTGGAGTACCCGCAGCTGAAGGGGACCTTCCCAGAGAATCTGAAGCATCTTAAGAACTCCATGGATGGCGTG


criterion=sequence-density
sequence-density=1.24
sequence-density-rank=1
fanout-score=3.46
fanout-score-rank=41
prefix-density=4.08
prefix-fanout=1.0
sequence=AGTACATGGGGG


criterion=fanout-score
sequence-density=0.15
sequence-density-rank=21
fanout-score=60.97
fanout-score-rank=1
prefix-density=8.71
prefix-fanout=1.0
sequence=ACGCAGAGTACT
SRR14016357 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 11 07:54:11
                             Started mapping on |	May 11 07:54:11
                                    Finished on |	May 11 07:56:54
       Mapping speed, Million of reads per hour |	631.65

                          Number of input reads |	28599650
                      Average input read length |	247
                                    UNIQUE READS:
                   Uniquely mapped reads number |	22419168
                        Uniquely mapped reads % |	78.39%
                          Average mapped length |	239.53
                       Number of splices: Total |	23079730
            Number of splices: Annotated (sjdb) |	22709879
                       Number of splices: GT/AG |	22792962
                       Number of splices: GC/AG |	166008
                       Number of splices: AT/AC |	22434
               Number of splices: Non-canonical |	98326
                      Mismatch rate per base, % |	0.34%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.55
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1424361
             % of reads mapped to multiple loci |	4.98%
        Number of reads mapped to too many loci |	91308
             % of reads mapped to too many loci |	0.32%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.19%
                     % of reads unmapped: other |	0.12%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4756121	4756121	4756121
N_multimapping	1424361	1424361	1424361
N_noFeature	800738	11389770	11050546
N_ambiguous	1399367	326829	342362
UnstrandedReadsAssigned:20219063 PositiveStrandReadsAssigned:10702569 NegativeStrandReadsAssigned:11026260
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=85 echo kmer=81
SRR14016357 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016357-trimmed-pair1.fastq
                             SRR14016357-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,599,650 reads, 23,495,103 reads pseudoaligned
[quant] estimated average fragment length: 158.543
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,042 rounds

 109283 SRR14016357.ke.tsv
  52636 SRR14016357.se.tsv
 161919 total
==> SRR14016357.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	6	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	1	0	0
ENSMUST00000178537.1	12	2	0	0
ENSMUST00000178862.1	14	4	0	0
ENSMUST00000179520.1	11	1	0	0
ENSMUST00000179883.1	16	6	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	2	0	0

==> SRR14016357.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR14016357 completed mapping pipeline successfully
