Starting /dee2/code/volunteer_pipeline.sh SRR14016369
    current disk space = 317269696
    free memory = 263329764 
SRR14016369 SRAfilesize
a4f8992ddf02bdd1604e698a1b60edc3  SRR14016369.sra
SRR14016369.sra file validated
SRR14016369 is paired end
SRR14016369 is conventional basespace
SRR14016369 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016369_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.294	37.0	37.0	37.0	37.0	37.0
2	36.27025	37.0	37.0	37.0	37.0	37.0
3	36.325	37.0	37.0	37.0	37.0	37.0
4	36.3335	37.0	37.0	37.0	37.0	37.0
5	36.4465	37.0	37.0	37.0	37.0	37.0
6	36.4045	37.0	37.0	37.0	37.0	37.0
7	36.294	37.0	37.0	37.0	37.0	37.0
8	36.3185	37.0	37.0	37.0	37.0	37.0
9	36.335	37.0	37.0	37.0	37.0	37.0
10-14	36.2855	37.0	37.0	37.0	37.0	37.0
15-19	36.298899999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.176300000000005	37.0	37.0	37.0	37.0	37.0
25-29	35.998599999999996	37.0	37.0	37.0	37.0	37.0
30-34	35.9405	37.0	37.0	37.0	37.0	37.0
35-39	35.8788	37.0	37.0	37.0	37.0	37.0
40-44	35.7643	37.0	37.0	37.0	37.0	37.0
45-49	35.6242	37.0	37.0	37.0	37.0	37.0
50-54	35.1811	37.0	37.0	37.0	34.6	37.0
55-59	33.8772	37.0	37.0	37.0	16.6	37.0
60-64	33.5738	37.0	37.0	37.0	16.6	37.0
65-69	33.152100000000004	37.0	37.0	37.0	11.0	37.0
70-74	32.8144	37.0	37.0	37.0	11.0	37.0
75-79	32.8722	37.0	37.0	37.0	11.0	37.0
80-84	32.8468	37.0	37.0	37.0	11.0	37.0
85-89	32.3198	37.0	37.0	37.0	11.0	37.0
90-94	32.1639	37.0	37.0	37.0	11.0	37.0
95-99	31.8161	37.0	37.0	37.0	11.0	37.0
100-104	31.732499999999998	37.0	37.0	37.0	11.0	37.0
105-109	31.5719	37.0	32.2	37.0	11.0	37.0
110-114	31.2755	37.0	25.0	37.0	11.0	37.0
115-119	31.0041	37.0	25.0	37.0	11.0	37.0
120-124	30.790999999999997	37.0	25.0	37.0	11.0	37.0
125-129	30.6125	37.0	25.0	37.0	11.0	37.0
130-134	30.2459	37.0	25.0	37.0	11.0	37.0
135-139	30.112600000000004	37.0	25.0	37.0	11.0	37.0
140-144	29.847500000000004	37.0	25.0	37.0	11.0	37.0
145-149	29.7442	37.0	25.0	37.0	11.0	37.0
150	29.4185	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	1.0
20	2.0
21	9.0
22	17.0
23	68.0
24	131.0
25	197.0
26	197.0
27	218.0
28	156.0
29	127.0
30	98.0
31	87.0
32	89.0
33	88.0
34	148.0
35	375.0
36	1847.0
37	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.475	25.8	15.525	30.2
2	17.38804103077308	29.221916437327994	29.44708531398549	23.942957217913435
3	24.175	23.9	30.4	21.525
4	20.5	32.125	20.0	27.375
5	22.15	40.125	20.025000000000002	17.7
6	28.549999999999997	28.9	24.825	17.724999999999998
7	21.125	24.5	31.6	22.775000000000002
8	28.075	27.150000000000002	23.724999999999998	21.05
9	26.075	22.55	30.475	20.9
10-14	20.405	30.409999999999997	26.43	22.755
15-19	24.435000000000002	27.634999999999998	24.33	23.599999999999998
20-24	24.23	28.860000000000003	28.13	18.78
25-29	19.945	25.380000000000003	33.365	21.310000000000002
30-34	20.655	24.884999999999998	35.54	18.92
35-39	18.475	24.955	38.315	18.255
40-44	19.74	22.965	39.82	17.474999999999998
45-49	19.0	24.759999999999998	39.405	16.835
50-54	19.02	24.92	38.2	17.86
55-59	20.655	26.875	33.19	19.28
60-64	19.775000000000002	31.06	30.049999999999997	19.115
65-69	20.080000000000002	31.275	30.154999999999998	18.490000000000002
70-74	20.48	32.035000000000004	28.815	18.67
75-79	22.96	30.91	26.75	19.38
80-84	22.285	31.814999999999998	26.029999999999998	19.869999999999997
85-89	20.669999999999998	33.76	24.279999999999998	21.29
90-94	20.45	33.775	24.69	21.085
95-99	19.82	34.98	23.64	21.560000000000002
100-104	18.87	36.69	21.91	22.53
105-109	18.615000000000002	36.195	22.95	22.24
110-114	18.015	36.945	22.759999999999998	22.28
115-119	18.7	35.415	23.200000000000003	22.685
120-124	19.28	34.155	23.665	22.900000000000002
125-129	18.634999999999998	33.945	23.14	24.279999999999998
130-134	18.655	33.295	24.385	23.665
135-139	19.15	32.01	24.765	24.075
140-144	20.31	32.464999999999996	24.29	22.935
145-149	21.560000000000002	30.645	25.35	22.445
150	24.55	29.375	23.65	22.425
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	2.0
3	0.5
4	0.5
5	1.0
6	2.5
7	4.5
8	6.0
9	5.5
10	5.5
11	6.0
12	12.5
13	20.5
14	22.0
15	26.0
16	27.5
17	30.5
18	30.0
19	27.5
20	32.5
21	44.5
22	43.5
23	38.0
24	44.5
25	51.5
26	57.5
27	62.0
28	70.0
29	70.5
30	71.0
31	74.0
32	68.5
33	75.0
34	88.0
35	82.0
36	66.0
37	66.5
38	75.5
39	92.5
40	106.0
41	121.5
42	107.0
43	94.5
44	137.0
45	158.0
46	171.5
47	177.5
48	146.5
49	114.5
50	121.0
51	137.0
52	151.5
53	158.5
54	117.0
55	81.0
56	74.0
57	57.5
58	47.0
59	44.0
60	45.0
61	39.5
62	20.5
63	13.5
64	17.5
65	16.5
66	9.5
67	3.0
68	1.0
69	2.0
70	2.0
71	1.0
72	0.0
73	0.0
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.075
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84816990510619	39.75
2	15.951197469498418	17.65
3	6.326253953908721	10.5
4	2.666064166290104	5.8999999999999995
5	1.2200632625395391	3.375
6	0.6326253953908721	2.1
7	0.4066877541798464	1.575
8	0.13556258472661548	0.6
9	0.09037505648441031	0.44999999999999996
>10	0.587437867148667	4.625
>50	0.045187528242205156	1.7500000000000002
>100	0.09037505648441031	11.725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	310	7.75	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	159	3.975	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	70	1.7500000000000002	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.975	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	20	0.5	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.4	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.325	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	11	0.27499999999999997	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	11	0.27499999999999997	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	11	0.27499999999999997	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	11	0.27499999999999997	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	10	0.25	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	10	0.25	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	9	0.22499999999999998	No Hit
CGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCA	8	0.2	No Hit
ACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAG	8	0.2	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	8	0.2	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	7	0.17500000000000002	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	7	0.17500000000000002	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	7	0.17500000000000002	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	7	0.17500000000000002	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	7	0.17500000000000002	No Hit
CTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTT	7	0.17500000000000002	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	7	0.17500000000000002	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	7	0.17500000000000002	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	6	0.15	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	6	0.15	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	6	0.15	No Hit
TGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGG	6	0.15	No Hit
GTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTG	6	0.15	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	6	0.15	No Hit
TGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACC	6	0.15	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	6	0.15	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	6	0.15	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	6	0.15	No Hit
CTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATG	6	0.15	No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
ATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGT	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	5	0.125	No Hit
CCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTC	5	0.125	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	5	0.125	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	5	0.125	No Hit
GTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAA	5	0.125	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	5	0.125	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	5	0.125	No Hit
CACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTT	5	0.125	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	5	0.125	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	5	0.125	No Hit
CTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGA	5	0.125	No Hit
GTGAAAACGCCTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCA	5	0.125	No Hit
GGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCC	5	0.125	No Hit
TCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAG	5	0.125	No Hit
CTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAG	5	0.125	No Hit
GGCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAG	5	0.125	No Hit
GTCAGGATGCATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGG	5	0.125	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	5	0.125	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	5	0.125	No Hit
TATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCT	5	0.125	No Hit
ATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCT	5	0.125	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	5	0.125	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	5	0.125	No Hit
GCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTC	5	0.125	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	5	0.125	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	5	0.125	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.025	0.0	0.0	0.0	0.0
36-37	0.025	0.0	0.0	0.0	0.0
38-39	0.025	0.0	0.0	0.0	0.0
40-41	0.037500000000000006	0.0	0.0	0.0	0.0
42-43	0.1375	0.0	0.0	0.0	0.0
44-45	0.21250000000000002	0.0	0.0	0.0	0.0
46-47	0.275	0.0	0.0	0.0	0.0
48-49	0.3375	0.0	0.0	0.0	0.0
50-51	0.4	0.0	0.0	0.0	0.0
52-53	0.42500000000000004	0.0	0.0	0.0	0.0
54-55	0.6	0.0	0.0	0.0	0.0
56-57	0.625	0.0	0.0	0.0	0.0
58-59	0.9625	0.0	0.0	0.0	0.0
60-61	1.2999999999999998	0.0	0.0	0.0	0.0
62-63	1.5625	0.0	0.0	0.0	0.0
64-65	1.8875	0.0	0.0	0.0	0.0
66-67	2.1625	0.0	0.0	0.0	0.0
68-69	2.675	0.0	0.0	0.0	0.0
70-71	3.0375	0.0	0.0	0.0	0.0
72-73	3.4125	0.0	0.0	0.0	0.0
74-75	3.9000000000000004	0.0	0.0	0.0	0.0
76-77	4.4125	0.0	0.0	0.0	0.0
78-79	4.8875	0.0	0.0	0.0	0.0
80-81	5.45	0.0	0.0	0.0	0.0
82-83	6.175000000000001	0.0	0.0	0.0	0.0
84-85	6.85	0.0	0.0	0.0	0.0
86-87	7.55	0.0	0.0	0.0	0.0
88-89	8.275	0.0	0.0	0.0	0.0
90-91	9.05	0.0	0.0	0.0	0.0
92-93	10.2875	0.0	0.0	0.0	0.0
94-95	11.2625	0.0	0.0	0.0	0.0
96-97	11.9875	0.0	0.0	0.0	0.0
98-99	12.962499999999999	0.0	0.0	0.0	0.0
100-101	13.8	0.0	0.0	0.0	0.0
102-103	14.2875	0.0	0.0	0.0	0.0
104-105	14.9625	0.0	0.0	0.0	0.0
106-107	15.649999999999999	0.0	0.0	0.0	0.0
108-109	16.525	0.0	0.0	0.0	0.0
110-111	17.1625	0.0	0.0	0.0	0.0
112-113	17.8875	0.0	0.0	0.0	0.0
114-115	18.6	0.0	0.0	0.0	0.0
116-117	19.200000000000003	0.0	0.0	0.0	0.0
118-119	20.0	0.0	0.0	0.0	0.0
120-121	20.6625	0.0	0.0	0.0	0.0
122-123	21.3125	0.0	0.0	0.0	0.0
124-125	21.875	0.0	0.0	0.0	0.0
126-127	22.375	0.0	0.0	0.0	0.0
128-129	23.125	0.0	0.0	0.0	0.0
130-131	23.7375	0.0	0.0	0.0	0.0
132-133	24.525	0.0	0.0	0.0	0.0
134-135	25.2875	0.0	0.0	0.0	0.0
136-137	25.95	0.0	0.0	0.0	0.0
138	26.425	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCAAG	10	0.006973645	144.0	9
GCTAATC	10	0.006973645	144.0	1
ATTGCAA	10	0.006973645	144.0	8
CTAATCA	10	0.006973645	144.0	2
TCATTGC	10	0.006973645	144.0	6
AAGCAGT	40	3.8780854E-9	108.0	1
AGCAGTG	45	8.794814E-9	96.0	2
TGGTATC	50	1.828812E-8	86.399994	7
AGTGGTA	50	1.828812E-8	86.399994	5
GTATCAA	50	1.828812E-8	86.399994	9
GTGGTAT	50	1.828812E-8	86.399994	6
CAGTGGT	50	1.828812E-8	86.399994	4
GGTATCA	50	1.828812E-8	86.399994	8
GCAGTGG	55	3.543937E-8	78.545456	3
CAGAGTA	65	2.1579981E-5	22.153845	9
GTACTTT	55	5.0997805E-6	20.945454	20-24
TCAACGC	55	5.0997805E-6	20.945454	10-14
AACGCAG	60	1.0824771E-5	19.199999	10-14
AGAGTAC	60	1.0824771E-5	19.199999	15-19
CAACGCA	60	1.0824771E-5	19.199999	10-14
>>END_MODULE
SRR14016369 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016369_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.00675	37.0	37.0	37.0	37.0	37.0
2	35.914	37.0	37.0	37.0	37.0	37.0
3	36.0175	37.0	37.0	37.0	37.0	37.0
4	35.9905	37.0	37.0	37.0	37.0	37.0
5	36.124	37.0	37.0	37.0	37.0	37.0
6	36.089	37.0	37.0	37.0	37.0	37.0
7	36.0375	37.0	37.0	37.0	37.0	37.0
8	36.162	37.0	37.0	37.0	37.0	37.0
9	36.1035	37.0	37.0	37.0	37.0	37.0
10-14	36.115300000000005	37.0	37.0	37.0	37.0	37.0
15-19	36.099199999999996	37.0	37.0	37.0	37.0	37.0
20-24	35.935900000000004	37.0	37.0	37.0	37.0	37.0
25-29	35.9163	37.0	37.0	37.0	37.0	37.0
30-34	35.9704	37.0	37.0	37.0	37.0	37.0
35-39	35.759699999999995	37.0	37.0	37.0	37.0	37.0
40-44	35.7162	37.0	37.0	37.0	37.0	37.0
45-49	35.4015	37.0	37.0	37.0	37.0	37.0
50-54	34.9643	37.0	37.0	37.0	29.8	37.0
55-59	33.6388	37.0	37.0	37.0	13.8	37.0
60-64	33.384499999999996	37.0	37.0	37.0	11.0	37.0
65-69	32.95790000000001	37.0	37.0	37.0	11.0	37.0
70-74	32.4326	37.0	37.0	37.0	11.0	37.0
75-79	32.4679	37.0	37.0	37.0	11.0	37.0
80-84	32.3523	37.0	37.0	37.0	11.0	37.0
85-89	31.6807	37.0	32.2	37.0	11.0	37.0
90-94	31.584600000000002	37.0	29.8	37.0	11.0	37.0
95-99	31.271300000000004	37.0	25.0	37.0	11.0	37.0
100-104	31.1064	37.0	25.0	37.0	11.0	37.0
105-109	30.870800000000003	37.0	25.0	37.0	11.0	37.0
110-114	30.655399999999997	37.0	25.0	37.0	11.0	37.0
115-119	30.2934	37.0	25.0	37.0	11.0	37.0
120-124	30.1312	37.0	25.0	37.0	11.0	37.0
125-129	30.0036	37.0	25.0	37.0	11.0	37.0
130-134	29.729599999999998	37.0	25.0	37.0	11.0	37.0
135-139	29.7231	37.0	25.0	37.0	11.0	37.0
140-144	29.386000000000003	37.0	22.2	37.0	11.0	37.0
145-149	29.493000000000002	37.0	25.0	37.0	11.0	37.0
150	29.409	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	1.0
17	1.0
18	2.0
19	3.0
20	5.0
21	6.0
22	32.0
23	84.0
24	185.0
25	234.0
26	219.0
27	178.0
28	120.0
29	106.0
30	77.0
31	95.0
32	93.0
33	104.0
34	163.0
35	573.0
36	1642.0
37	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.49637227920941	27.570678008506377	14.83612709532149	29.09682261696272
2	17.775	30.3	27.425	24.5
3	24.55	24.825	30.5	20.125
4	20.05	33.5	18.4	28.050000000000004
5	21.775	40.925	20.65	16.650000000000002
6	29.225	27.6	24.975	18.2
7	20.925	23.225	33.75	22.1
8	27.925	28.549999999999997	22.925	20.599999999999998
9	25.575	22.45	32.074999999999996	19.900000000000002
10-14	21.310000000000002	30.135	26.035000000000004	22.52
15-19	24.325	27.43	24.279999999999998	23.965
20-24	23.645	28.54	29.21	18.605
25-29	19.725	25.415	33.375	21.485000000000003
30-34	20.84	24.665	35.725	18.77
35-39	18.310000000000002	24.47	38.85	18.37
40-44	19.335	23.855	39.085	17.724999999999998
45-49	19.035	24.654999999999998	39.14	17.169999999999998
50-54	19.025	26.279999999999998	37.015	17.68
55-59	21.115000000000002	27.51	32.71	18.665000000000003
60-64	20.23	30.825000000000003	30.459999999999997	18.485
65-69	20.26	31.545	30.509999999999998	17.685000000000002
70-74	21.235	31.605	28.82	18.34
75-79	23.44	30.680000000000003	27.305	18.575
80-84	23.565	31.740000000000002	26.91	17.785
85-89	22.79	33.405	24.349999999999998	19.455
90-94	23.25	34.544999999999995	23.91	18.295
95-99	23.200000000000003	34.865	23.615	18.32
100-104	22.42	36.575	22.875	18.13
105-109	23.27	36.14	23.580000000000002	17.01
110-114	23.54	36.614999999999995	23.415	16.43
115-119	24.97	35.39	23.72	15.920000000000002
120-124	25.990000000000002	34.765	24.01	15.235000000000001
125-129	25.915	34.410000000000004	23.075000000000003	16.6
130-134	27.01	34.365	22.74	15.885
135-139	28.105000000000004	33.48	22.384999999999998	16.03
140-144	29.695	34.82	21.445	14.04
145-149	31.574999999999996	33.56	21.98	12.885
150	34.25	31.45	22.15	12.15
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	1.5
5	1.5
6	2.0
7	6.5
8	8.0
9	7.0
10	5.5
11	5.0
12	14.5
13	24.5
14	30.0
15	28.5
16	27.0
17	31.0
18	31.0
19	29.0
20	37.0
21	44.5
22	40.0
23	51.0
24	61.0
25	54.0
26	52.5
27	57.0
28	71.5
29	70.0
30	60.5
31	77.5
32	82.5
33	73.5
34	74.0
35	68.5
36	57.0
37	64.5
38	68.5
39	76.5
40	104.5
41	132.0
42	121.5
43	102.5
44	117.0
45	128.0
46	153.0
47	179.0
48	164.0
49	131.5
50	126.5
51	148.5
52	153.5
53	140.5
54	107.5
55	85.0
56	74.0
57	52.0
58	50.5
59	52.5
60	47.5
61	34.5
62	19.0
63	13.0
64	15.0
65	15.0
66	12.5
67	7.0
68	3.0
69	4.5
70	3.0
71	0.5
72	0.0
73	0.5
74	1.0
75	1.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.474999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64276228419655	41.025
2	15.714918105356352	17.75
3	6.330234617087206	10.725
4	2.1248339973439574	4.8
5	1.2837538733953076	3.6249999999999996
6	0.619743249225321	2.1
7	0.2656042496679947	1.05
8	0.35413899955732625	1.6
9	0.04426737494466578	0.22499999999999998
>10	0.5312084993359893	5.5
>50	0.0	0.0
>100	0.08853474988933156	11.600000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	304	7.6	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	160	4.0	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	1.2	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	44	1.0999999999999999	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	21	0.525	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	14	0.35000000000000003	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.35000000000000003	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.325	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	13	0.325	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	12	0.3	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	11	0.27499999999999997	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	10	0.25	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	10	0.25	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	10	0.25	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	8	0.2	No Hit
GTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTG	8	0.2	No Hit
CCACCGAGGTCGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTC	8	0.2	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	8	0.2	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	8	0.2	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	8	0.2	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	8	0.2	No Hit
CCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCC	7	0.17500000000000002	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	7	0.17500000000000002	No Hit
CAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGC	7	0.17500000000000002	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	7	0.17500000000000002	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	7	0.17500000000000002	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	7	0.17500000000000002	No Hit
CTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGT	6	0.15	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	6	0.15	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	6	0.15	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	6	0.15	No Hit
CAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCC	6	0.15	No Hit
TTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	6	0.15	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	6	0.15	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	6	0.15	No Hit
CTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTAC	6	0.15	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	6	0.15	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	5	0.125	No Hit
CAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAATATTAATT	5	0.125	No Hit
GCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAA	5	0.125	No Hit
TGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGG	5	0.125	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	5	0.125	No Hit
GATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGT	5	0.125	No Hit
GTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAA	5	0.125	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	5	0.125	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	5	0.125	No Hit
AGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCT	5	0.125	No Hit
ATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCC	5	0.125	No Hit
AGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCC	5	0.125	No Hit
TGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACC	5	0.125	No Hit
TGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCG	5	0.125	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	5	0.125	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	5	0.125	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	5	0.125	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	5	0.125	No Hit
TCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGG	5	0.125	No Hit
GACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAACAATTTA	5	0.125	No Hit
GATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGC	5	0.125	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	5	0.125	No Hit
GACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAA	5	0.125	No Hit
GGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAACAATTT	5	0.125	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	5	0.125	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	5	0.125	No Hit
TTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTG	5	0.125	No Hit
ATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTG	5	0.125	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.025	0.0	0.0	0.0	0.0
36-37	0.025	0.0	0.0	0.0	0.0
38-39	0.025	0.0	0.0	0.0	0.0
40-41	0.037500000000000006	0.0	0.0	0.0	0.0
42-43	0.1375	0.0	0.0	0.0	0.0
44-45	0.21250000000000002	0.0	0.0	0.0	0.0
46-47	0.275	0.0	0.0	0.0	0.0
48-49	0.3375	0.0	0.0	0.0	0.0
50-51	0.375	0.0	0.0	0.0	0.0
52-53	0.4	0.0	0.0	0.0	0.0
54-55	0.6	0.0	0.0	0.0	0.0
56-57	0.625	0.0	0.0	0.0	0.0
58-59	0.9375	0.0	0.0	0.0	0.0
60-61	1.275	0.0	0.0	0.0	0.0
62-63	1.5375	0.0	0.0	0.0	0.0
64-65	1.875	0.0	0.0	0.0	0.0
66-67	2.1625	0.0	0.0	0.0	0.0
68-69	2.675	0.0	0.0	0.0	0.0
70-71	3.0375	0.0	0.0	0.0	0.0
72-73	3.4000000000000004	0.0	0.0	0.0	0.0
74-75	3.9000000000000004	0.0	0.0	0.0	0.0
76-77	4.425	0.0	0.0	0.0	0.0
78-79	4.9	0.0	0.0	0.0	0.0
80-81	5.4375	0.0	0.0	0.0	0.0
82-83	6.125	0.0	0.0	0.0	0.0
84-85	6.8125	0.0	0.0	0.0	0.0
86-87	7.4875	0.0	0.0	0.0	0.0
88-89	8.1875	0.0	0.0	0.0	0.0
90-91	8.975	0.0	0.0	0.0	0.0
92-93	10.2125	0.0	0.0	0.0	0.0
94-95	11.1375	0.0	0.0	0.0	0.0
96-97	11.8375	0.0	0.0	0.0	0.0
98-99	12.7875	0.0	0.0	0.0	0.0
100-101	13.6	0.0	0.0	0.0	0.0
102-103	14.0875	0.0	0.0	0.0	0.0
104-105	14.7625	0.0	0.0	0.0	0.0
106-107	15.4875	0.0	0.0	0.0	0.0
108-109	16.3625	0.0	0.0	0.0	0.0
110-111	16.9625	0.0	0.0	0.0	0.0
112-113	17.675	0.0	0.0	0.0	0.0
114-115	18.4375	0.0	0.0	0.0	0.0
116-117	19.075000000000003	0.0	0.0	0.0	0.0
118-119	19.85	0.0	0.0	0.0	0.0
120-121	20.512500000000003	0.0	0.0	0.0	0.0
122-123	21.15	0.0	0.0	0.0	0.0
124-125	21.674999999999997	0.0	0.0	0.0	0.0
126-127	22.1875	0.0	0.0	0.0	0.0
128-129	22.9625	0.0	0.0	0.0	0.0
130-131	23.6125	0.0	0.0	0.0	0.0
132-133	24.425	0.0	0.0	0.0	0.0
134-135	25.2	0.0	0.0	0.0	0.0
136-137	25.8625	0.0	0.0	0.0	0.0
138	26.35	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCAAG	45	0.0	144.0	9
CATTGCA	45	0.0	144.0	7
AATCATT	45	0.0	144.0	4
GCTAATC	45	0.0	144.0	1
ATTGCAA	45	0.0	144.0	8
CTAATCA	45	0.0	144.0	2
TAATCAT	45	0.0	144.0	3
ATCATTG	45	0.0	144.0	5
TCATTGC	45	0.0	144.0	6
AAGCAGT	80	6.017217E-9	63.0	1
AGCAGTG	85	9.71886E-9	59.294117	2
GCAGTGG	90	1.5266778E-8	56.0	3
TGGTATC	95	2.339948E-8	53.05263	7
AGTGGTA	95	2.339948E-8	53.05263	5
GTGGTAT	95	2.339948E-8	53.05263	6
CAGTGGT	95	2.339948E-8	53.05263	4
GGTATCA	95	2.339948E-8	53.05263	8
GTATCAA	100	3.5073754E-8	50.399998	9
GCAAGCA	45	2.7146598E-8	28.8	10-14
CAAGCAG	45	2.7146598E-8	28.8	10-14
>>END_MODULE
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:27:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:27:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:27:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:27:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:27:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:27:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:27:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:27:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:27:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:28:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
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2023-05-11T05:28:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:28:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T05:28:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
Read 571641 spots for SRR14016369.sra
Written 571641 spots for SRR14016369.sra
2023-05-11T05:28:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 571652 spots for SRR14016369.sra
Written 571652 spots for SRR14016369.sra
SRR ids: ['SRR14016369.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_df1fgmu5
SRR14016369.sra spots: 36585035
blocks: [[1, 571641], [571642, 1143282], [1143283, 1714923], [1714924, 2286564], [2286565, 2858205], [2858206, 3429846], [3429847, 4001487], [4001488, 4573128], [4573129, 5144769], [5144770, 5716410], [5716411, 6288051], [6288052, 6859692], [6859693, 7431333], [7431334, 8002974], [8002975, 8574615], [8574616, 9146256], [9146257, 9717897], [9717898, 10289538], [10289539, 10861179], [10861180, 11432820], [11432821, 12004461], [12004462, 12576102], [12576103, 13147743], [13147744, 13719384], [13719385, 14291025], [14291026, 14862666], [14862667, 15434307], [15434308, 16005948], [16005949, 16577589], [16577590, 17149230], [17149231, 17720871], [17720872, 18292512], [18292513, 18864153], [18864154, 19435794], [19435795, 20007435], [20007436, 20579076], [20579077, 21150717], [21150718, 21722358], [21722359, 22293999], [22294000, 22865640], [22865641, 23437281], [23437282, 24008922], [24008923, 24580563], [24580564, 25152204], [25152205, 25723845], [25723846, 26295486], [26295487, 26867127], [26867128, 27438768], [27438769, 28010409], [28010410, 28582050], [28582051, 29153691], [29153692, 29725332], [29725333, 30296973], [30296974, 30868614], [30868615, 31440255], [31440256, 32011896], [32011897, 32583537], [32583538, 33155178], [33155179, 33726819], [33726820, 34298460], [34298461, 34870101], [34870102, 35441742], [35441743, 36013383], [36013384, 36585035]]
SRR14016369 file size 12340044
SRR14016369 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016369 SRR14016369_1.fastq SRR14016369_2.fastq
Input file:	SRR14016369_1.fastq
Paired file:	SRR14016369_2.fastq
trimmed:	SRR14016369-trimmed-pair1.fastq, SRR14016369-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 05:29:02 2023 >> started

Thu May 11 05:30:17 2023 >> done (75.603s)
36585035 read pairs processed; of these:
    1231 ( 0.00%) short read pairs filtered out after trimming by size control
    9071 ( 0.02%) empty read pairs filtered out after trimming by size control
36574733 (99.97%) read pairs available; of these:
10499184 (28.71%) trimmed read pairs available after processing
26075549 (71.29%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      95	  0.00%
 19	      51	  0.00%
 20	     118	  0.00%
 21	     152	  0.00%
 22	     440	  0.00%
 23	     466	  0.00%
 24	     439	  0.00%
 25	     547	  0.00%
 26	     743	  0.00%
 27	    1138	  0.00%
 28	    1638	  0.00%
 29	    2433	  0.01%
 30	    3422	  0.01%
 31	    4703	  0.01%
 32	    5669	  0.02%
 33	    5832	  0.02%
 34	    6848	  0.02%
 35	    6662	  0.02%
 36	    7141	  0.02%
 37	    7933	  0.02%
 38	    8284	  0.02%
 39	    9336	  0.03%
 40	    9427	  0.03%
 41	   10483	  0.03%
 42	   12944	  0.04%
 43	   12493	  0.03%
 44	   13251	  0.04%
 45	   13683	  0.04%
 46	   14364	  0.04%
 47	   15189	  0.04%
 48	   16173	  0.04%
 49	   17157	  0.05%
 50	   18803	  0.05%
 51	   19848	  0.05%
 52	   21137	  0.06%
 53	   22804	  0.06%
 54	   24155	  0.07%
 55	   25523	  0.07%
 56	   26669	  0.07%
 57	   27880	  0.08%
 58	   29154	  0.08%
 59	   30086	  0.08%
 60	   31507	  0.09%
 61	   33284	  0.09%
 62	   35087	  0.10%
 63	   36487	  0.10%
 64	   38407	  0.11%
 65	   39884	  0.11%
 66	   41670	  0.11%
 67	   43424	  0.12%
 68	   45056	  0.12%
 69	   46466	  0.13%
 70	   48740	  0.13%
 71	   50492	  0.14%
 72	   52713	  0.14%
 73	   55255	  0.15%
 74	   57100	  0.16%
 75	   59562	  0.16%
 76	   61533	  0.17%
 77	   62424	  0.17%
 78	   65109	  0.18%
 79	   67199	  0.18%
 80	   68562	  0.19%
 81	   71084	  0.19%
 82	   73482	  0.20%
 83	   75894	  0.21%
 84	   77882	  0.21%
 85	   80194	  0.22%
 86	   82316	  0.23%
 87	   84459	  0.23%
 88	   86487	  0.24%
 89	   88067	  0.24%
 90	   90142	  0.25%
 91	   92672	  0.25%
 92	   94618	  0.26%
 93	   97340	  0.27%
 94	   99664	  0.27%
 95	  101285	  0.28%
 96	  104004	  0.28%
 97	  105696	  0.29%
 98	  108577	  0.30%
 99	  109300	  0.30%
100	  111018	  0.30%
101	  113583	  0.31%
102	  115215	  0.32%
103	  117884	  0.32%
104	  119641	  0.33%
105	  121592	  0.33%
106	  122998	  0.34%
107	  124718	  0.34%
108	  127151	  0.35%
109	  128438	  0.35%
110	  129753	  0.35%
111	  131456	  0.36%
112	  133886	  0.37%
113	  135787	  0.37%
114	  137000	  0.37%
115	  139290	  0.38%
116	  139477	  0.38%
117	  141462	  0.39%
118	  142988	  0.39%
119	  143759	  0.39%
120	  144296	  0.39%
121	  146571	  0.40%
122	  146743	  0.40%
123	  148195	  0.41%
124	  150611	  0.41%
125	  151515	  0.41%
126	  151618	  0.41%
127	  152802	  0.42%
128	  152251	  0.42%
129	  154606	  0.42%
130	  155487	  0.43%
131	  155145	  0.42%
132	  155458	  0.43%
133	  157738	  0.43%
134	  157742	  0.43%
135	  158266	  0.43%
136	  159957	  0.44%
137	  160215	  0.44%
138	  158909	  0.43%
139	  160359	  0.44%
140	  160697	  0.44%
141	  162000	  0.44%
142	  161449	  0.44%
143	  161474	  0.44%
144	  160018	  0.44%
145	  162051	  0.44%
146	  162514	  0.44%
147	  162509	  0.44%
148	  162111	  0.44%
149	  170344	  0.47%
150	26075549	 71.29%
36574733 reads passed initial QC


criterion=sequence-density
sequence-density=3.84
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=44
prefix-density=0.01
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=50.03
fanout-score-rank=1
prefix-density=0.45
prefix-fanout=1.2
sequence=GCCTGTAATTGATCATTCTCAAGGCATTCAGATAGTCAGGTGAGAGTCGACTTAGGGTATAGGACATACGCCATGACAGGGATCCTATAAGGAAAACGTGCTAGACATCAGCAAACAGGCAGAACTCATTGGCATGAAGATCTGGGCCCTGGGGCATAGCTAGGGAGATGAGATGGGTCAGGAATATTGATGTAATCATCTGAATCAGGGTCTTCCAGGTTGACAGCAGGCTGGTAGCCCAAGCTCACGTATTCACAGCTCATCTTCAGAGATGGGGTGTGGGGCCAAGGAGCTTCAAGTACCTGCGGAGAAGCGGGGGACGCTGAGGCTGGGGCGTTGAGGAGCGGAGCCTCCGCTGGACCCAAGCTGTCTGGTCCCCGTGCGCGCCGGGGGCAGGCGCTGTAGACGTTGTTCTGCGAGCGCGGCCTCTGCGAGGCATCCCGGCGCTGTGTGGGGAACGGGCGGGAAGCCCCCCGGCCTCGCCTCCTCATCGCTAGTCGGCCCGCACGTC


criterion=sequence-density
sequence-density=3.73
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=45
prefix-density=0.01
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=57.89
fanout-score-rank=1
prefix-density=0.44
prefix-fanout=1.1
sequence=GCCTGTAATTGATCATTCTCAAGGCATTCAGATAGTCAGGTGAGAGTCGACTTAGGGTATAGGACATACGCCATGACAGGGATCCTATAAGGAAAACGTGCTAGACATCAGCAAACAGGCAGAACTCATTGGCATGAAGATCTGGGCCCTGGGGCATAGCTAGGGAGATGAGATGGGTCAGGAATATTGATGTAATCATCTGAATCAGGGTCTTCCAGGTTGACAGCAGGCTGGTAGCCCAAGCTCACGTATTCACAGCTCATCTTCAGAGATGGGGTGTGGGGCCAAGGAGCTTCAAGTACCTGCGGAGAAGCGGGGGACGCTGAGGCTGGGGCGTTGAGGAGCGGAGCCTCCGCTGGACCCAAGCTGTCTGGTCCCCGTGCGCGCCGGGGGCAGGCGCTGTAGACGTTGTTCTGCGAGCGCGGCCTCTGCGAGGCATCCCGGCGCTGTGTGGGGAACGGGCGGGAAGCCCCCCGGCCTCGCCTCCTCATCGCTAGTCGGCCCGCACGTC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG -y GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG -o SRR14016369 SRR14016369_1.fastq SRR14016369_2.fastq
Input file:	SRR14016369_1.fastq
Paired file:	SRR14016369_2.fastq
trimmed:	SRR14016369-trimmed-pair1.fastq, SRR14016369-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG
-- paired 3' end adapter sequence (-y):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 05:34:30 2023 >> started

Thu May 11 05:35:10 2023 >> done (39.818s)
18287367 read pairs processed; of these:
     288 ( 0.00%) short read pairs filtered out after trimming by size control
 1052215 ( 5.75%) empty read pairs filtered out after trimming by size control
17234864 (94.24%) read pairs available; of these:
    1057 ( 0.01%) trimmed read pairs available after processing
17233807 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      46	  0.00%
 19	      17	  0.00%
 20	      52	  0.00%
 21	      59	  0.00%
 22	     200	  0.00%
 23	     206	  0.00%
 24	     190	  0.00%
 25	     200	  0.00%
 26	     359	  0.00%
 27	     552	  0.00%
 28	     748	  0.00%
 29	    1121	  0.01%
 30	    1587	  0.01%
 31	    2225	  0.01%
 32	    2761	  0.02%
 33	    2698	  0.02%
 34	    2756	  0.02%
 35	    2633	  0.02%
 36	    3353	  0.02%
 37	    3670	  0.02%
 38	    3999	  0.02%
 39	    4510	  0.03%
 40	    4418	  0.03%
 41	    4714	  0.03%
 42	    5269	  0.03%
 43	    5869	  0.03%
 44	    6196	  0.04%
 45	    6491	  0.04%
 46	    6656	  0.04%
 47	    7179	  0.04%
 48	    7733	  0.04%
 49	    8034	  0.05%
 50	    8839	  0.05%
 51	    9106	  0.05%
 52	    9725	  0.06%
 53	   10620	  0.06%
 54	   11459	  0.07%
 55	   11962	  0.07%
 56	   12594	  0.07%
 57	   13034	  0.08%
 58	   13872	  0.08%
 59	   14239	  0.08%
 60	   15079	  0.09%
 61	   15876	  0.09%
 62	   16783	  0.10%
 63	   17279	  0.10%
 64	   18462	  0.11%
 65	   19135	  0.11%
 66	   19747	  0.11%
 67	   20735	  0.12%
 68	   21604	  0.13%
 69	   22307	  0.13%
 70	   23468	  0.14%
 71	   24205	  0.14%
 72	   25181	  0.15%
 73	   26490	  0.15%
 74	   27250	  0.16%
 75	   28716	  0.17%
 76	   29293	  0.17%
 77	   29789	  0.17%
 78	   31148	  0.18%
 79	   32333	  0.19%
 80	   32941	  0.19%
 81	   34037	  0.20%
 82	   35461	  0.21%
 83	   36359	  0.21%
 84	   36869	  0.21%
 85	   38129	  0.22%
 86	   39545	  0.23%
 87	   40239	  0.23%
 88	   41730	  0.24%
 89	   42174	  0.24%
 90	   43225	  0.25%
 91	   43935	  0.25%
 92	   45044	  0.26%
 93	   46356	  0.27%
 94	   47689	  0.28%
 95	   48000	  0.28%
 96	   49534	  0.29%
 97	   50356	  0.29%
 98	   51585	  0.30%
 99	   52275	  0.30%
100	   52869	  0.31%
101	   53784	  0.31%
102	   54402	  0.32%
103	   55467	  0.32%
104	   57033	  0.33%
105	   57406	  0.33%
106	   57623	  0.33%
107	   59282	  0.34%
108	   60038	  0.35%
109	   60815	  0.35%
110	   61851	  0.36%
111	   62551	  0.36%
112	   62930	  0.37%
113	   64434	  0.37%
114	   64533	  0.37%
115	   66009	  0.38%
116	   66120	  0.38%
117	   66781	  0.39%
118	   67507	  0.39%
119	   68130	  0.40%
120	   68463	  0.40%
121	   69199	  0.40%
122	   69518	  0.40%
123	   69893	  0.41%
124	   71171	  0.41%
125	   71147	  0.41%
126	   70850	  0.41%
127	   71783	  0.42%
128	   71910	  0.42%
129	   73127	  0.42%
130	   73385	  0.43%
131	   73402	  0.43%
132	   73399	  0.43%
133	   73770	  0.43%
134	   74183	  0.43%
135	   74639	  0.43%
136	   75533	  0.44%
137	   75334	  0.44%
138	   74940	  0.43%
139	   75114	  0.44%
140	   75744	  0.44%
141	   75927	  0.44%
142	   75763	  0.44%
143	   75837	  0.44%
144	   75336	  0.44%
145	   76008	  0.44%
146	   76407	  0.44%
147	   76714	  0.45%
148	   76695	  0.44%
149	   80392	  0.47%
150	12267402	 71.18%


criterion=sequence-density
sequence-density=1.21
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=40
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=32
fanout-score=196.81
fanout-score-rank=1
prefix-density=0.57
prefix-fanout=18.4
sequence=CTGCTGCTGTGGCTTACAGATGCCAGATGTGACATCCAGA


criterion=sequence-density
sequence-density=0.41
sequence-density-rank=1
fanout-score=2.99
fanout-score-rank=35
prefix-density=1.17
prefix-fanout=1.0
sequence=AGTACATGGGGG


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=31
fanout-score=204.52
fanout-score-rank=1
prefix-density=0.58
prefix-fanout=19.2
sequence=CTGCTGCTGTGGCTTACAGATGCCAGATGTGACATCCAGA
SRR14016369 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 11 05:38:12
                             Started mapping on |	May 11 05:38:13
                                    Finished on |	May 11 05:41:18
       Mapping speed, Million of reads per hour |	691.24

                          Number of input reads |	35522230
                      Average input read length |	277
                                    UNIQUE READS:
                   Uniquely mapped reads number |	29248713
                        Uniquely mapped reads % |	82.34%
                          Average mapped length |	272.41
                       Number of splices: Total |	30166885
            Number of splices: Annotated (sjdb) |	29665547
                       Number of splices: GT/AG |	29825694
                       Number of splices: GC/AG |	219207
                       Number of splices: AT/AC |	35883
               Number of splices: Non-canonical |	86101
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.69
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.40
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1392283
             % of reads mapped to multiple loci |	3.92%
        Number of reads mapped to too many loci |	134438
             % of reads mapped to too many loci |	0.38%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.18%
                     % of reads unmapped: other |	0.18%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4881237	4881237	4881237
N_multimapping	1392283	1392283	1392283
N_noFeature	2975844	15609202	15534380
N_ambiguous	1699805	334295	335801
UnstrandedReadsAssigned:24573064 PositiveStrandReadsAssigned:13305216 NegativeStrandReadsAssigned:13378532
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=129 echo kmer=125
SRR14016369 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016369-trimmed-pair1.fastq
                             SRR14016369-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 35,522,230 reads, 27,240,993 reads pseudoaligned
[quant] estimated average fragment length: 199.15
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,205 rounds

 109283 SRR14016369.ke.tsv
  52636 SRR14016369.se.tsv
 161919 total
==> SRR14016369.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	11.6	0	0
ENSMUST00000196221.1	9	4.6	0	0
ENSMUST00000179664.1	11	6.6	0	0
ENSMUST00000178537.1	12	7.6	0	0
ENSMUST00000178862.1	14	9.6	0	0
ENSMUST00000179520.1	11	6.6	0	0
ENSMUST00000179883.1	16	11.6	0	0
ENSMUST00000195858.1	10	5.6	0	0
ENSMUST00000179932.1	12	7.6	0	0

==> SRR14016369.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR14016369 completed mapping pipeline successfully
