Starting /dee2/code/volunteer_pipeline.sh SRR14016363
    current disk space = 315975184
    free memory = 263188068 
SRR14016363 SRAfilesize
9f34f6a29655a267d7db6a45a40622d1  SRR14016363.sra
SRR14016363.sra file validated
SRR14016363 is paired end
SRR14016363 is conventional basespace
SRR14016363 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.511	37.0	37.0	37.0	37.0	37.0
2	35.33725	37.0	37.0	37.0	37.0	37.0
3	35.448	37.0	37.0	37.0	37.0	37.0
4	35.6815	37.0	37.0	37.0	37.0	37.0
5	35.7335	37.0	37.0	37.0	37.0	37.0
6	35.6425	37.0	37.0	37.0	37.0	37.0
7	35.6615	37.0	37.0	37.0	37.0	37.0
8	35.7275	37.0	37.0	37.0	37.0	37.0
9	35.51625	37.0	37.0	37.0	37.0	37.0
10-14	35.680800000000005	37.0	37.0	37.0	37.0	37.0
15-19	35.577	37.0	37.0	37.0	37.0	37.0
20-24	35.4677	37.0	37.0	37.0	37.0	37.0
25-29	35.19035	37.0	37.0	37.0	29.8	37.0
30-34	35.1327	37.0	37.0	37.0	27.4	37.0
35-39	34.832300000000004	37.0	37.0	37.0	25.0	37.0
40-44	34.7247	37.0	37.0	37.0	25.0	37.0
45-49	34.5672	37.0	37.0	37.0	25.0	37.0
50-54	34.1071	37.0	37.0	37.0	25.0	37.0
55-59	33.2985	37.0	37.0	37.0	13.8	37.0
60-64	32.8637	37.0	37.0	37.0	11.0	37.0
65-69	32.1592	37.0	34.6	37.0	11.0	37.0
70-74	31.4809	37.0	25.0	37.0	11.0	37.0
75-79	31.5036	37.0	25.0	37.0	11.0	37.0
80-84	31.7024	37.0	34.6	37.0	11.0	37.0
85-89	31.225299999999997	37.0	25.0	37.0	11.0	37.0
90-94	30.9885	37.0	25.0	37.0	11.0	37.0
95-99	30.5637	37.0	25.0	37.0	11.0	37.0
100-104	30.2656	37.0	25.0	37.0	11.0	37.0
105-109	30.0298	37.0	25.0	37.0	11.0	37.0
110-114	29.701	37.0	25.0	37.0	11.0	37.0
115-119	29.447699999999998	37.0	22.2	37.0	11.0	37.0
120-124	29.284699999999997	37.0	19.4	37.0	11.0	37.0
125-129	28.7805	37.0	11.0	37.0	11.0	37.0
130-134	28.5017	37.0	11.0	37.0	11.0	37.0
135-139	28.3565	37.0	11.0	37.0	11.0	37.0
140-144	28.2245	37.0	11.0	37.0	11.0	37.0
145-149	28.2952	37.0	11.0	37.0	11.0	37.0
150	28.2285	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	9.0
21	16.0
22	58.0
23	106.0
24	157.0
25	233.0
26	235.0
27	212.0
28	195.0
29	172.0
30	147.0
31	149.0
32	146.0
33	178.0
34	261.0
35	520.0
36	1134.0
37	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.416208104052025	23.011505752876438	14.532266133066532	30.040020010005
2	16.195244055068837	24.755944931163956	28.260325406758447	30.78848560700876
3	21.675	21.75	34.075	22.5
4	18.925	37.1	17.875	26.1
5	21.325	41.775	18.5	18.4
6	26.224999999999998	26.200000000000003	30.099999999999998	17.474999999999998
7	20.1	23.200000000000003	27.875	28.825
8	25.6	33.050000000000004	19.825	21.525
9	23.40585146286572	22.080520130032507	33.9584896224056	20.555138784696176
10-14	22.405	29.104999999999997	24.884999999999998	23.605
15-19	25.195	26.14	24.145	24.52
20-24	24.959999999999997	27.279999999999998	28.689999999999998	19.07
25-29	20.361018050902548	26.866343317165857	28.89644482224111	23.876193809690484
30-34	23.474999999999998	25.61	30.37	20.544999999999998
35-39	18.11	25.135	36.47	20.285
40-44	20.535	22.845	38.29	18.33
45-49	18.515	24.805	38.86	17.82
50-54	19.45	25.865	36.82	17.865000000000002
55-59	21.634999999999998	25.900000000000002	33.894999999999996	18.57
60-64	21.445	29.035	30.714999999999996	18.805
65-69	21.95	30.255	29.270000000000003	18.525
70-74	22.495	32.16	25.564999999999998	19.78
75-79	23.28	32.95	23.215	20.555
80-84	22.965	31.735000000000003	25.080000000000002	20.22
85-89	22.96	32.515	22.165000000000003	22.36
90-94	22.905	32.995000000000005	21.175	22.925
95-99	21.39	34.239999999999995	19.79	24.58
100-104	19.485	35.9	19.505	25.11
105-109	19.23	35.115	21.029999999999998	24.625
110-114	19.605	34.54	20.66	25.195
115-119	19.915	33.62	21.07	25.395
120-124	21.560000000000002	33.47	20.565	24.404999999999998
125-129	21.685	32.36	20.7	25.255
130-134	24.905	31.945	19.735	23.415
135-139	27.389999999999997	30.455	18.865000000000002	23.29
140-144	29.175	31.014999999999997	19.72	20.09
145-149	32.11142228445689	29.145829165833163	19.248849769953992	19.49389877975595
150	36.15	26.0	17.825	20.025000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	1.0
3	0.5
4	2.5
5	3.5
6	3.0
7	2.5
8	2.0
9	2.0
10	4.5
11	9.5
12	10.5
13	9.0
14	10.0
15	15.0
16	22.0
17	28.0
18	27.5
19	27.0
20	34.5
21	41.0
22	41.5
23	43.5
24	45.0
25	39.5
26	50.5
27	65.0
28	71.5
29	68.5
30	68.5
31	80.5
32	80.0
33	79.5
34	89.5
35	107.5
36	106.5
37	90.5
38	79.5
39	66.5
40	53.5
41	52.5
42	57.0
43	52.0
44	58.0
45	75.0
46	90.0
47	109.0
48	121.5
49	117.5
50	133.0
51	159.0
52	174.0
53	182.0
54	161.0
55	147.5
56	121.0
57	89.5
58	96.5
59	99.0
60	86.5
61	64.5
62	43.0
63	28.0
64	28.5
65	31.5
66	17.0
67	7.0
68	6.0
69	3.0
70	1.0
71	0.5
72	0.0
73	0.0
74	1.5
75	2.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.025
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.02
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11421098517872	44.224999999999994
2	12.380122057541412	14.2
3	4.882301656495205	8.4
4	2.3975588491717525	5.5
5	1.0897994768962511	3.125
6	0.6974716652136007	2.4
7	0.26155187445510025	1.05
8	0.13077593722755013	0.6
9	0.17436791630340018	0.8999999999999999
>10	0.7846556233653008	6.875000000000001
>50	0.0	0.0
>100	0.08718395815170009	12.725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	309	7.725	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	200	5.0	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	46	1.15	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.8	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.65	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	15	0.375	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	14	0.35000000000000003	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	12	0.3	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	12	0.3	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	12	0.3	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.3	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	11	0.27499999999999997	No Hit
GCCAGAGGCTGGTGATGGGACCACCGAGATCTCACCCACTGGTGCTGCTG	11	0.27499999999999997	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	11	0.27499999999999997	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	10	0.25	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	10	0.25	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	10	0.25	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	10	0.25	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	10	0.25	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	9	0.22499999999999998	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	9	0.22499999999999998	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	9	0.22499999999999998	No Hit
CTTTGATTGGTCAGGATGCATTTTTTCTGGAACAGACCAAGAAGAAAGGC	8	0.2	No Hit
ATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGCC	8	0.2	No Hit
ATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTC	8	0.2	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	7	0.17500000000000002	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	7	0.17500000000000002	No Hit
CCACCGAGGTCGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTC	7	0.17500000000000002	No Hit
CTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACG	7	0.17500000000000002	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	7	0.17500000000000002	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	7	0.17500000000000002	No Hit
CGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCA	6	0.15	No Hit
GGCTTCTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATC	6	0.15	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	6	0.15	No Hit
GCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGAT	6	0.15	No Hit
GTCCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGA	6	0.15	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	6	0.15	No Hit
CTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGT	6	0.15	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTT	6	0.15	No Hit
CCTGACCAATCAAAGGCGTGTCCAGAGGGTTGTCTGGGTTCCAGAGGTCA	6	0.15	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	6	0.15	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	6	0.15	No Hit
CTCAGAGCTGAGCTGCACATCAATGTCAGGAGCCTGGTACTTGAGCCGTC	6	0.15	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	6	0.15	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	6	0.15	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	5	0.125	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	5	0.125	No Hit
AGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGT	5	0.125	No Hit
GAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGC	5	0.125	No Hit
GTCCTGGGGCCCAGGTTTGGCCTTTGGTGTGGGAGGGCTAAGGAGTCGGG	5	0.125	No Hit
CAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CTCAGGACACTGAAGCCAGAAGGTCACATTCTCCGAGACAGGTTCAAGAG	5	0.125	No Hit
GTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAA	5	0.125	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGTAGAG	5	0.125	No Hit
GTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCA	5	0.125	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	5	0.125	No Hit
GTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGA	5	0.125	No Hit
GGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCC	5	0.125	No Hit
CTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGT	5	0.125	No Hit
CCCTCTTTCACTGGAAGGGGCAAGATAAGAATGCCCCCACAGAAGGTAAC	5	0.125	No Hit
CGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATA	5	0.125	No Hit
AGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTG	5	0.125	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	5	0.125	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	5	0.125	No Hit
CCCCAAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAA	5	0.125	No Hit
TCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGA	5	0.125	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	5	0.125	No Hit
ATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGT	5	0.125	No Hit
TTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTG	5	0.125	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0875	0.0	0.0	0.0	0.0
32-33	0.23750000000000002	0.0	0.0	0.0	0.0
34-35	0.3375	0.0	0.0	0.0	0.0
36-37	0.42500000000000004	0.0	0.0	0.0	0.0
38-39	0.5	0.0	0.0	0.0	0.0
40-41	0.675	0.0	0.0	0.0	0.0
42-43	0.875	0.0	0.0	0.0	0.0
44-45	1.0875	0.0	0.0	0.0	0.0
46-47	1.275	0.0	0.0	0.0	0.0
48-49	1.6375000000000002	0.0	0.0	0.0	0.0
50-51	1.9500000000000002	0.0	0.0	0.0	0.0
52-53	2.1625	0.0	0.0	0.0	0.0
54-55	2.6625	0.0	0.0	0.0	0.0
56-57	3.4875	0.0	0.0	0.0	0.0
58-59	4.4	0.0	0.0	0.0	0.0
60-61	5.125	0.0	0.0	0.0	0.0
62-63	6.075	0.0	0.0	0.0	0.0
64-65	7.35	0.0	0.0	0.0	0.0
66-67	8.524999999999999	0.0	0.0	0.0	0.0
68-69	9.8125	0.0	0.0	0.0	0.0
70-71	10.8875	0.0	0.0	0.0	0.0
72-73	11.7875	0.0	0.0	0.0	0.0
74-75	13.1375	0.0	0.0	0.0	0.0
76-77	14.275	0.0	0.0	0.0	0.0
78-79	15.3	0.0	0.0	0.0	0.0
80-81	16.2375	0.0	0.0	0.0	0.0
82-83	17.6625	0.0	0.0	0.0	0.0
84-85	19.3125	0.0	0.0	0.0	0.0
86-87	20.625	0.0	0.0	0.0	0.0
88-89	22.075000000000003	0.0	0.0	0.0	0.0
90-91	23.375	0.0	0.0	0.0	0.0
92-93	25.575	0.0	0.0	0.0	0.0
94-95	27.1	0.0	0.0	0.0	0.0
96-97	28.625	0.0	0.0	0.0	0.0
98-99	30.425	0.0	0.0	0.0	0.0
100-101	31.2875	0.0	0.0	0.0	0.0
102-103	32.0375	0.0	0.0	0.0	0.0
104-105	32.6125	0.0	0.0	0.0	0.0
106-107	33.275	0.0	0.0	0.0	0.0
108-109	34.1	0.0	0.0	0.0	0.0
110-111	34.75	0.0	0.0	0.0	0.0
112-113	35.2625	0.0	0.0	0.0	0.0
114-115	35.8625	0.0	0.0	0.0	0.0
116-117	36.35	0.0	0.0	0.0	0.0
118-119	36.8375	0.0	0.0	0.0	0.0
120-121	37.4	0.0	0.0	0.0	0.0
122-123	37.9	0.0	0.0	0.0	0.0
124-125	38.474999999999994	0.0	0.0	0.0	0.0
126-127	39.1125	0.0	0.0	0.0	0.0
128-129	39.575	0.0	0.0	0.0	0.0
130-131	40.3625	0.0	0.0	0.0	0.0
132-133	41.087500000000006	0.0	0.0	0.0	0.0
134-135	41.7625	0.0	0.0	0.0	0.0
136-137	42.337500000000006	0.0	0.0	0.0	0.0
138	42.65	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	15	1.1150019E-4	145.81013	1
TTGCAAG	20	3.689142E-4	107.99063	9
CATTGCA	20	3.689142E-4	107.99063	7
ATTGCAA	20	3.689142E-4	107.99063	8
CTAATCA	20	3.689142E-4	107.99063	2
TAATCAT	20	3.689142E-4	107.99063	3
TCATTGC	20	3.689142E-4	107.99063	6
AATCATT	25	8.959314E-4	86.392494	4
ATCATTG	25	8.959314E-4	86.392494	5
AAGCAGT	40	5.716207E-5	72.90507	1
AGCAGTG	40	6.093763E-5	71.99375	2
AGTGGTA	45	1.0921781E-4	63.994446	5
CAGTGGT	45	1.0921781E-4	63.994446	4
TGGTATC	50	1.8396128E-4	57.594997	7
GTATCAA	50	1.8396128E-4	57.594997	9
GCAGTGG	50	1.8396128E-4	57.594997	3
GTGGTAT	50	1.8396128E-4	57.594997	6
GGTATCA	50	1.8396128E-4	57.594997	8
GCAAGCA	20	0.006141849	28.797503	10-14
GGGGGGG	725	0.0	8.937155	140-144
>>END_MODULE
SRR14016363 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016363_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.73575	37.0	37.0	37.0	25.0	37.0
2	34.402	37.0	37.0	37.0	25.0	37.0
3	34.742	37.0	37.0	37.0	25.0	37.0
4	35.1245	37.0	37.0	37.0	25.0	37.0
5	35.443	37.0	37.0	37.0	37.0	37.0
6	35.161	37.0	37.0	37.0	37.0	37.0
7	35.1605	37.0	37.0	37.0	25.0	37.0
8	35.2865	37.0	37.0	37.0	37.0	37.0
9	35.295	37.0	37.0	37.0	37.0	37.0
10-14	35.3311	37.0	37.0	37.0	37.0	37.0
15-19	35.2845	37.0	37.0	37.0	34.6	37.0
20-24	35.1841	37.0	37.0	37.0	29.8	37.0
25-29	35.0457	37.0	37.0	37.0	25.0	37.0
30-34	34.9698	37.0	37.0	37.0	25.0	37.0
35-39	34.7156	37.0	37.0	37.0	25.0	37.0
40-44	34.663799999999995	37.0	37.0	37.0	25.0	37.0
45-49	34.2944	37.0	37.0	37.0	25.0	37.0
50-54	33.9408	37.0	37.0	37.0	22.2	37.0
55-59	33.053700000000006	37.0	37.0	37.0	13.8	37.0
60-64	32.6725	37.0	37.0	37.0	11.0	37.0
65-69	32.009	37.0	34.6	37.0	11.0	37.0
70-74	31.369999999999997	37.0	25.0	37.0	11.0	37.0
75-79	31.495300000000004	37.0	25.0	37.0	11.0	37.0
80-84	31.323	37.0	25.0	37.0	11.0	37.0
85-89	30.7786	37.0	25.0	37.0	11.0	37.0
90-94	30.788	37.0	25.0	37.0	11.0	37.0
95-99	30.288400000000003	37.0	25.0	37.0	11.0	37.0
100-104	30.048000000000002	37.0	25.0	37.0	11.0	37.0
105-109	29.8664	37.0	25.0	37.0	11.0	37.0
110-114	29.617499999999996	37.0	25.0	37.0	11.0	37.0
115-119	29.366899999999998	37.0	25.0	37.0	11.0	37.0
120-124	29.165999999999997	37.0	22.2	37.0	11.0	37.0
125-129	28.899	37.0	13.8	37.0	11.0	37.0
130-134	28.624599999999997	37.0	11.0	37.0	11.0	37.0
135-139	28.4775	37.0	11.0	37.0	11.0	37.0
140-144	28.406799999999997	37.0	11.0	37.0	11.0	37.0
145-149	28.3748	37.0	11.0	37.0	11.0	37.0
150	28.2525	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	5.0
20	12.0
21	28.0
22	60.0
23	126.0
24	214.0
25	242.0
26	220.0
27	209.0
28	155.0
29	131.0
30	114.0
31	125.0
32	134.0
33	200.0
34	347.0
35	630.0
36	1019.0
37	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.08277069267317	23.905976494123532	13.353338334583645	31.657914478619652
2	15.275	25.374999999999996	29.875	29.475
3	21.45	21.275	34.075	23.200000000000003
4	20.825	35.225	17.825	26.125
5	21.75	42.65	17.05	18.55
6	25.75	27.975	27.275	19.0
7	21.45	24.25	25.775	28.525
8	25.575	32.4	20.75	21.275
9	24.5	22.025	33.074999999999996	20.4
10-14	21.995	28.79	24.965	24.25
15-19	25.365	26.51	23.96	24.165
20-24	25.145	27.529999999999998	28.035	19.29
25-29	20.880000000000003	26.900000000000002	28.43	23.79
30-34	22.865	26.32	30.345	20.47
35-39	18.26	25.665	36.065000000000005	20.01
40-44	20.93	24.060000000000002	36.57	18.44
45-49	19.545	25.264999999999997	37.685	17.505000000000003
50-54	20.325	26.640000000000004	35.099999999999994	17.935000000000002
55-59	22.735	26.985	32.015	18.265
60-64	22.23	29.275000000000002	29.609999999999996	18.884999999999998
65-69	23.549999999999997	31.095	28.355000000000004	17.0
70-74	24.295	31.924999999999997	26.115	17.665
75-79	26.88	31.830000000000002	23.810000000000002	17.48
80-84	27.405	31.2	24.88	16.515
85-89	27.55	32.155	22.825	17.47
90-94	28.405	33.934999999999995	21.97	15.690000000000001
95-99	28.365000000000002	34.28	21.39	15.964999999999998
100-104	27.650000000000002	35.56	20.77	16.02
105-109	28.42	35.325	21.41	14.845
110-114	27.894999999999996	36.665	21.075	14.365
115-119	29.244999999999997	36.885	20.945	12.925
120-124	30.865	35.74	20.845	12.55
125-129	31.44	35.375	20.375	12.809999999999999
130-134	34.715	33.425	18.925	12.934999999999999
135-139	36.86	32.66	17.580000000000002	12.9
140-144	38.99	32.86	17.095	11.055
145-149	40.725	32.269999999999996	16.7	10.305
150	43.125	31.35	16.950000000000003	8.575000000000001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.5
2	2.0
3	1.5
4	2.5
5	2.5
6	2.5
7	3.0
8	3.5
9	4.0
10	7.0
11	8.5
12	9.0
13	14.0
14	16.0
15	19.5
16	23.5
17	28.0
18	37.5
19	38.5
20	38.5
21	39.0
22	40.0
23	39.5
24	40.0
25	47.5
26	52.0
27	51.5
28	53.0
29	61.0
30	70.0
31	66.5
32	63.0
33	79.5
34	90.0
35	78.5
36	77.5
37	78.0
38	74.0
39	74.0
40	60.0
41	56.0
42	65.5
43	67.5
44	65.5
45	68.5
46	92.0
47	122.0
48	130.5
49	141.5
50	152.5
51	155.5
52	167.0
53	188.0
54	169.5
55	152.0
56	126.0
57	87.5
58	92.5
59	97.5
60	89.5
61	55.0
62	27.5
63	28.5
64	33.0
65	31.0
66	19.5
67	6.5
68	2.0
69	1.5
70	1.0
71	0.5
72	0.0
73	0.5
74	0.5
75	1.0
76	1.0
77	0.5
78	0.5
79	0.0
80	0.0
81	0.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34736842105264	45.925
2	12.589473684210525	14.95
3	4.589473684210526	8.175
4	2.189473684210526	5.2
5	1.0105263157894737	3.0
6	0.5894736842105264	2.1
7	0.5894736842105264	2.45
8	0.2947368421052632	1.4000000000000001
9	0.12631578947368421	0.675
>10	0.5473684210526315	4.475
>50	0.042105263157894736	1.35
>100	0.08421052631578947	10.299999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	232	5.800000000000001	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	180	4.5	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	54	1.35	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.7250000000000001	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	21	0.525	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.44999999999999996	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	15	0.375	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	12	0.3	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	11	0.27499999999999997	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	11	0.27499999999999997	No Hit
ATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTC	11	0.27499999999999997	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	11	0.27499999999999997	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	10	0.25	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	10	0.25	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	10	0.25	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	10	0.25	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	9	0.22499999999999998	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTAA	9	0.22499999999999998	No Hit
TTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGA	9	0.22499999999999998	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	8	0.2	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	8	0.2	No Hit
ATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	8	0.2	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	8	0.2	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	8	0.2	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	8	0.2	No Hit
CCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.17500000000000002	No Hit
GCCAGAGGCTGGTGATGGGACCACCGAGATCTCACCCACTGGTGCTGCTG	7	0.17500000000000002	No Hit
ATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGCC	7	0.17500000000000002	No Hit
CAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGC	7	0.17500000000000002	No Hit
CTCACACATCTCCCGAAACACGGACTCCTGGGTGGCAGCTTGCTCTGAGG	7	0.17500000000000002	No Hit
TGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACC	7	0.17500000000000002	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	7	0.17500000000000002	No Hit
CCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGCCTTTCTTC	7	0.17500000000000002	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	7	0.17500000000000002	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
GGTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACG	7	0.17500000000000002	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	7	0.17500000000000002	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	7	0.17500000000000002	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	6	0.15	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	6	0.15	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGACA	6	0.15	No Hit
CCTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACCAGACCAG	6	0.15	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	6	0.15	No Hit
GAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGG	6	0.15	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGC	6	0.15	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTCGAGCCGCGCT	6	0.15	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	6	0.15	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	6	0.15	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCT	6	0.15	No Hit
ATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCTC	5	0.125	No Hit
CATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCT	5	0.125	No Hit
GTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTGGCT	5	0.125	No Hit
TGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAG	5	0.125	No Hit
TGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGG	5	0.125	No Hit
GCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAA	5	0.125	No Hit
CTCACGGTAGGCCCGCTTCTCCACCAGCTTCACTTTGTATTTGCGCTTGA	5	0.125	No Hit
CACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTCTAT	5	0.125	No Hit
CCACCGAGGTCGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTC	5	0.125	No Hit
CTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGA	5	0.125	No Hit
GTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAA	5	0.125	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGTAGAG	5	0.125	No Hit
CTTTATTGAGGGGCGGGGCTCCGACATCTGCACAGCTACAACTGAATCTC	5	0.125	No Hit
CTCCGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCG	5	0.125	No Hit
TTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCG	5	0.125	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	5	0.125	No Hit
GTGAAAACGCCTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCA	5	0.125	No Hit
GTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGA	5	0.125	No Hit
CAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTCTATGAC	5	0.125	No Hit
ACTGAAGCCAGAAGGTCACATTCTCCGAGACAGGTTCAAGAGCTTCCAGA	5	0.125	No Hit
CTCAGAGCTGAGCTGCACATCAATGTCAGGAGCCTGGTACTTGAGCCGTC	5	0.125	No Hit
CTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATG	5	0.125	No Hit
CAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAA	5	0.125	No Hit
CCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTCTATGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0875	0.0	0.0	0.0	0.0
32-33	0.23750000000000002	0.0	0.0	0.0	0.0
34-35	0.3375	0.0	0.0	0.0	0.0
36-37	0.42500000000000004	0.0	0.0	0.0	0.0
38-39	0.5	0.0	0.0	0.0	0.0
40-41	0.675	0.0	0.0	0.0	0.0
42-43	0.9125	0.0	0.0	0.0	0.0
44-45	1.1625	0.0	0.0	0.0	0.0
46-47	1.35	0.0	0.0	0.0	0.0
48-49	1.6875	0.0	0.0	0.0	0.0
50-51	1.9875	0.0	0.0	0.0	0.0
52-53	2.1875	0.0	0.0	0.0	0.0
54-55	2.7	0.0	0.0	0.0	0.0
56-57	3.4875	0.0	0.0	0.0	0.0
58-59	4.35	0.0	0.0	0.0	0.0
60-61	5.112500000000001	0.0	0.0	0.0	0.0
62-63	6.0625	0.0	0.0	0.0	0.0
64-65	7.3625	0.0	0.0	0.0	0.0
66-67	8.5375	0.0	0.0	0.0	0.0
68-69	9.725000000000001	0.0	0.0	0.0	0.0
70-71	10.7875	0.0	0.0	0.0	0.0
72-73	11.7125	0.0	0.0	0.0	0.0
74-75	12.9875	0.0	0.0	0.0	0.0
76-77	14.0625	0.0	0.0	0.0	0.0
78-79	15.037500000000001	0.0	0.0	0.0	0.0
80-81	15.9375	0.0	0.0	0.0	0.0
82-83	17.325000000000003	0.0	0.0	0.0	0.0
84-85	18.9375	0.0	0.0	0.0	0.0
86-87	20.3375	0.0	0.0	0.0	0.0
88-89	21.775	0.0	0.0	0.0	0.0
90-91	23.1125	0.0	0.0	0.0	0.0
92-93	25.262500000000003	0.0	0.0	0.0	0.0
94-95	26.75	0.0	0.0	0.0	0.0
96-97	28.2875	0.0	0.0	0.0	0.0
98-99	30.0	0.0	0.0	0.0	0.0
100-101	30.8375	0.0	0.0	0.0	0.0
102-103	31.55	0.0	0.0	0.0	0.0
104-105	32.05	0.0	0.0	0.0	0.0
106-107	32.724999999999994	0.0	0.0	0.0	0.0
108-109	33.475	0.0	0.0	0.0	0.0
110-111	34.125	0.0	0.0	0.0	0.0
112-113	34.6375	0.0	0.0	0.0	0.0
114-115	35.175	0.0	0.0	0.0	0.0
116-117	35.6125	0.0	0.0	0.0	0.0
118-119	36.1125	0.0	0.0	0.0	0.0
120-121	36.662499999999994	0.0	0.0	0.0	0.0
122-123	37.1875	0.0	0.0	0.0	0.0
124-125	37.7125	0.0	0.0	0.0	0.0
126-127	38.275000000000006	0.0	0.0	0.0	0.0
128-129	38.75	0.0	0.0	0.0	0.0
130-131	39.474999999999994	0.0	0.0	0.0	0.0
132-133	40.162499999999994	0.0	0.0	0.0	0.0
134-135	40.8625	0.0	0.0	0.0	0.0
136-137	41.4	0.0	0.0	0.0	0.0
138	41.725	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	65	0.0	143.99998	1
CTAATCA	65	0.0	143.99998	2
TCATTGC	65	0.0	143.99998	6
CATTGCA	70	0.0	133.71428	7
TAATCAT	70	0.0	133.71428	3
AATCATT	75	0.0	124.799995	4
ATTGCAA	75	0.0	124.799995	8
ATCATTG	75	0.0	124.799995	5
TTGCAAG	80	0.0	117.0	9
ACATGGG	25	5.183459E-4	28.8	35-39
GTACATG	25	5.183459E-4	28.8	30-34
TACATGG	25	5.183459E-4	28.8	35-39
AGTACAT	25	5.183459E-4	28.8	30-34
GAGTACA	25	5.183459E-4	28.8	30-34
CATGGGG	25	5.183459E-4	28.8	35-39
TGCAAGC	75	1.0913936E-11	24.959997	10-14
GCAAGCA	80	2.3646862E-11	23.4	10-14
CAAGCAG	80	2.3646862E-11	23.4	10-14
AAGCAGT	85	5.4569682E-11	22.02353	10-14
AGCAGTG	90	1.1823431E-10	20.8	10-14
>>END_MODULE
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:15:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:15:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:15:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:15:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:15:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
2023-05-11T08:16:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T08:16:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 454367 spots for SRR14016363.sra
Written 454367 spots for SRR14016363.sra
Read 454369 spots for SRR14016363.sra
Written 454369 spots for SRR14016363.sra
SRR ids: ['SRR14016363.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_huljh671
SRR14016363.sra spots: 29079490
blocks: [[1, 454367], [454368, 908734], [908735, 1363101], [1363102, 1817468], [1817469, 2271835], [2271836, 2726202], [2726203, 3180569], [3180570, 3634936], [3634937, 4089303], [4089304, 4543670], [4543671, 4998037], [4998038, 5452404], [5452405, 5906771], [5906772, 6361138], [6361139, 6815505], [6815506, 7269872], [7269873, 7724239], [7724240, 8178606], [8178607, 8632973], [8632974, 9087340], [9087341, 9541707], [9541708, 9996074], [9996075, 10450441], [10450442, 10904808], [10904809, 11359175], [11359176, 11813542], [11813543, 12267909], [12267910, 12722276], [12722277, 13176643], [13176644, 13631010], [13631011, 14085377], [14085378, 14539744], [14539745, 14994111], [14994112, 15448478], [15448479, 15902845], [15902846, 16357212], [16357213, 16811579], [16811580, 17265946], [17265947, 17720313], [17720314, 18174680], [18174681, 18629047], [18629048, 19083414], [19083415, 19537781], [19537782, 19992148], [19992149, 20446515], [20446516, 20900882], [20900883, 21355249], [21355250, 21809616], [21809617, 22263983], [22263984, 22718350], [22718351, 23172717], [23172718, 23627084], [23627085, 24081451], [24081452, 24535818], [24535819, 24990185], [24990186, 25444552], [25444553, 25898919], [25898920, 26353286], [26353287, 26807653], [26807654, 27262020], [27262021, 27716387], [27716388, 28170754], [28170755, 28625121], [28625122, 29079490]]
SRR14016363 file size 9803992
SRR14016363 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016363 SRR14016363_1.fastq SRR14016363_2.fastq
Input file:	SRR14016363_1.fastq
Paired file:	SRR14016363_2.fastq
trimmed:	SRR14016363-trimmed-pair1.fastq, SRR14016363-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 08:17:06 2023 >> started

Thu May 11 08:18:09 2023 >> done (62.625s)
29079490 read pairs processed; of these:
    3161 ( 0.01%) short read pairs filtered out after trimming by size control
    3058 ( 0.01%) empty read pairs filtered out after trimming by size control
29073271 (99.98%) read pairs available; of these:
12365120 (42.53%) trimmed read pairs available after processing
16708151 (57.47%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     264	  0.00%
 19	     249	  0.00%
 20	     474	  0.00%
 21	     678	  0.00%
 22	    1498	  0.01%
 23	    1153	  0.00%
 24	    1214	  0.00%
 25	    1629	  0.01%
 26	    2402	  0.01%
 27	    3829	  0.01%
 28	    5842	  0.02%
 29	    8260	  0.03%
 30	   11432	  0.04%
 31	   16383	  0.06%
 32	   21867	  0.08%
 33	   21091	  0.07%
 34	   26330	  0.09%
 35	   24626	  0.08%
 36	   24059	  0.08%
 37	   27507	  0.09%
 38	   28743	  0.10%
 39	   33575	  0.12%
 40	   34005	  0.12%
 41	   37710	  0.13%
 42	   50911	  0.18%
 43	   43444	  0.15%
 44	   45553	  0.16%
 45	   46573	  0.16%
 46	   48933	  0.17%
 47	   50871	  0.17%
 48	   54061	  0.19%
 49	   57506	  0.20%
 50	   60733	  0.21%
 51	   64263	  0.22%
 52	   68120	  0.23%
 53	   72222	  0.25%
 54	   74062	  0.25%
 55	   76860	  0.26%
 56	   78409	  0.27%
 57	   79774	  0.27%
 58	   81461	  0.28%
 59	   82821	  0.28%
 60	   84318	  0.29%
 61	   86545	  0.30%
 62	   88967	  0.31%
 63	   91820	  0.32%
 64	   94000	  0.32%
 65	   95389	  0.33%
 66	   97767	  0.34%
 67	   98563	  0.34%
 68	  100775	  0.35%
 69	  101135	  0.35%
 70	  104055	  0.36%
 71	  105139	  0.36%
 72	  107328	  0.37%
 73	  110118	  0.38%
 74	  111220	  0.38%
 75	  112074	  0.39%
 76	  114036	  0.39%
 77	  114764	  0.39%
 78	  114310	  0.39%
 79	  116216	  0.40%
 80	  116815	  0.40%
 81	  117383	  0.40%
 82	  119876	  0.41%
 83	  120540	  0.41%
 84	  120753	  0.42%
 85	  122510	  0.42%
 86	  123748	  0.43%
 87	  123690	  0.43%
 88	  125092	  0.43%
 89	  124088	  0.43%
 90	  124713	  0.43%
 91	  125567	  0.43%
 92	  127139	  0.44%
 93	  127344	  0.44%
 94	  127789	  0.44%
 95	  128596	  0.44%
 96	  129808	  0.45%
 97	  130534	  0.45%
 98	  130057	  0.45%
 99	  130685	  0.45%
100	  130217	  0.45%
101	  130888	  0.45%
102	  131061	  0.45%
103	  131540	  0.45%
104	  131580	  0.45%
105	  131147	  0.45%
106	  132231	  0.45%
107	  131112	  0.45%
108	  132231	  0.45%
109	  131299	  0.45%
110	  131436	  0.45%
111	  131300	  0.45%
112	  131376	  0.45%
113	  132072	  0.45%
114	  131968	  0.45%
115	  132282	  0.45%
116	  131468	  0.45%
117	  131186	  0.45%
118	  131291	  0.45%
119	  130119	  0.45%
120	  129522	  0.45%
121	  129465	  0.45%
122	  128693	  0.44%
123	  129508	  0.45%
124	  130550	  0.45%
125	  129030	  0.44%
126	  128099	  0.44%
127	  128373	  0.44%
128	  128265	  0.44%
129	  126679	  0.44%
130	  126952	  0.44%
131	  124920	  0.43%
132	  123906	  0.43%
133	  125522	  0.43%
134	  124681	  0.43%
135	  124121	  0.43%
136	  123229	  0.42%
137	  123862	  0.43%
138	  121860	  0.42%
139	  121987	  0.42%
140	  120696	  0.42%
141	  120462	  0.41%
142	  119534	  0.41%
143	  119101	  0.41%
144	  116875	  0.40%
145	  116871	  0.40%
146	  116374	  0.40%
147	  117401	  0.40%
148	  118151	  0.41%
149	  125964	  0.43%
150	16708151	 57.47%
29073271 reads passed initial QC


criterion=sequence-density
sequence-density=5.54
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=39
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=58.22
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=3.4
sequence=CTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGTTTTATCCAACTCATCCCTTGACATCGTGCTTCACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGGCTTCACCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTA


criterion=sequence-density
sequence-density=1.43
sequence-density-rank=1
fanout-score=31.56
fanout-score-rank=3
prefix-density=1.46
prefix-fanout=30.8
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA


criterion=fanout-score
sequence-density=0.15
sequence-density-rank=23
fanout-score=74.01
fanout-score-rank=1
prefix-density=10.78
prefix-fanout=1.0
sequence=ACGCAGAGTACT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG -y CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA -o SRR14016363 SRR14016363_1.fastq SRR14016363_2.fastq
Input file:	SRR14016363_1.fastq
Paired file:	SRR14016363_2.fastq
trimmed:	SRR14016363-trimmed-pair1.fastq, SRR14016363-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG
-- paired 3' end adapter sequence (-y):	CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 08:21:22 2023 >> started

Thu May 11 08:21:51 2023 >> done (29.537s)
14536636 read pairs processed; of these:
     153 ( 0.00%) short read pairs filtered out after trimming by size control
  541542 ( 3.73%) empty read pairs filtered out after trimming by size control
13994941 (96.27%) read pairs available; of these:
    1150 ( 0.01%) trimmed read pairs available after processing
13993791 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      96	  0.00%
 19	     117	  0.00%
 20	     208	  0.00%
 21	     283	  0.00%
 22	     718	  0.01%
 23	     571	  0.00%
 24	     624	  0.00%
 25	     823	  0.01%
 26	    1256	  0.01%
 27	    1903	  0.01%
 28	    2850	  0.02%
 29	    4080	  0.03%
 30	    5638	  0.04%
 31	    8000	  0.06%
 32	   10171	  0.07%
 33	   10156	  0.07%
 34	   11323	  0.08%
 35	   11155	  0.08%
 36	   11741	  0.08%
 37	   13002	  0.09%
 38	   13862	  0.10%
 39	   15412	  0.11%
 40	   16426	  0.12%
 41	   17973	  0.13%
 42	   22323	  0.16%
 43	   20644	  0.15%
 44	   21554	  0.15%
 45	   22526	  0.16%
 46	   23688	  0.17%
 47	   24844	  0.18%
 48	   26619	  0.19%
 49	   28165	  0.20%
 50	   29714	  0.21%
 51	   31233	  0.22%
 52	   33068	  0.24%
 53	   34441	  0.25%
 54	   36020	  0.26%
 55	   36800	  0.26%
 56	   38051	  0.27%
 57	   38283	  0.27%
 58	   39633	  0.28%
 59	   40129	  0.29%
 60	   40777	  0.29%
 61	   42034	  0.30%
 62	   42997	  0.31%
 63	   44412	  0.32%
 64	   45597	  0.33%
 65	   46132	  0.33%
 66	   47142	  0.34%
 67	   47762	  0.34%
 68	   48813	  0.35%
 69	   49207	  0.35%
 70	   50099	  0.36%
 71	   50611	  0.36%
 72	   51614	  0.37%
 73	   52966	  0.38%
 74	   54157	  0.39%
 75	   53911	  0.39%
 76	   54962	  0.39%
 77	   55551	  0.40%
 78	   55374	  0.40%
 79	   56123	  0.40%
 80	   56507	  0.40%
 81	   56441	  0.40%
 82	   57796	  0.41%
 83	   58610	  0.42%
 84	   58182	  0.42%
 85	   59394	  0.42%
 86	   60080	  0.43%
 87	   60415	  0.43%
 88	   60210	  0.43%
 89	   59971	  0.43%
 90	   60658	  0.43%
 91	   60715	  0.43%
 92	   61492	  0.44%
 93	   61401	  0.44%
 94	   61165	  0.44%
 95	   61479	  0.44%
 96	   62461	  0.45%
 97	   62649	  0.45%
 98	   62292	  0.45%
 99	   63180	  0.45%
100	   63335	  0.45%
101	   63248	  0.45%
102	   63459	  0.45%
103	   63316	  0.45%
104	   63423	  0.45%
105	   62766	  0.45%
106	   64042	  0.46%
107	   63523	  0.45%
108	   64426	  0.46%
109	   63892	  0.46%
110	   63359	  0.45%
111	   63719	  0.46%
112	   62971	  0.45%
113	   63835	  0.46%
114	   63561	  0.45%
115	   63686	  0.46%
116	   63470	  0.45%
117	   63399	  0.45%
118	   63402	  0.45%
119	   62171	  0.44%
120	   62250	  0.44%
121	   62103	  0.44%
122	   62117	  0.44%
123	   61974	  0.44%
124	   62704	  0.45%
125	   61947	  0.44%
126	   62708	  0.45%
127	   61739	  0.44%
128	   61565	  0.44%
129	   60941	  0.44%
130	   61067	  0.44%
131	   60041	  0.43%
132	   59646	  0.43%
133	   60698	  0.43%
134	   59684	  0.43%
135	   59638	  0.43%
136	   59374	  0.42%
137	   59534	  0.43%
138	   58744	  0.42%
139	   58730	  0.42%
140	   57626	  0.41%
141	   57536	  0.41%
142	   57347	  0.41%
143	   57042	  0.41%
144	   56249	  0.40%
145	   56016	  0.40%
146	   55888	  0.40%
147	   56460	  0.40%
148	   57142	  0.41%
149	   60689	  0.43%
150	 8035277	 57.42%


criterion=sequence-density
sequence-density=2.23
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=28
prefix-density=0.01
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=26
fanout-score=71.42
fanout-score-rank=1
prefix-density=0.20
prefix-fanout=12.6
sequence=GGAGGAGGAGAAGGAGGTGGGGAGGAGGTTTGGAGCACCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACATTGATGTCTTTGGGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGCATCAGCCCGTTTTATTTCCAGCTTGGTCCCCCCTCCGAACGTGTACGGGCTCTCACC


criterion=sequence-density
sequence-density=1.40
sequence-density-rank=1
fanout-score=3.20
fanout-score-rank=42
prefix-density=4.34
prefix-fanout=1.0
sequence=AGTACATGGGGG


criterion=fanout-score
sequence-density=0.17
sequence-density-rank=20
fanout-score=60.70
fanout-score-rank=1
prefix-density=10.04
prefix-fanout=1.0
sequence=ACGCAGAGTACT
SRR14016363 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 11 08:24:36
                             Started mapping on |	May 11 08:24:37
                                    Finished on |	May 11 08:27:23
       Mapping speed, Million of reads per hour |	618.76

                          Number of input reads |	28531576
                      Average input read length |	256
                                    UNIQUE READS:
                   Uniquely mapped reads number |	21812887
                        Uniquely mapped reads % |	76.45%
                          Average mapped length |	248.37
                       Number of splices: Total |	21932358
            Number of splices: Annotated (sjdb) |	21525275
                       Number of splices: GT/AG |	21666367
                       Number of splices: GC/AG |	151676
                       Number of splices: AT/AC |	24683
               Number of splices: Non-canonical |	89632
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1483040
             % of reads mapped to multiple loci |	5.20%
        Number of reads mapped to too many loci |	77345
             % of reads mapped to too many loci |	0.27%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	17.90%
                     % of reads unmapped: other |	0.18%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5235649	5235649	5235649
N_multimapping	1483040	1483040	1483040
N_noFeature	1499165	11408991	11038212
N_ambiguous	1503244	332636	349842
UnstrandedReadsAssigned:18810478 PositiveStrandReadsAssigned:10071260 NegativeStrandReadsAssigned:10424833
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=98 echo kmer=93
SRR14016363 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016363-trimmed-pair1.fastq
                             SRR14016363-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,531,576 reads, 21,873,747 reads pseudoaligned
[quant] estimated average fragment length: 160.395
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,063 rounds

 109283 SRR14016363.ke.tsv
  52636 SRR14016363.se.tsv
 161919 total
==> SRR14016363.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	5	0	0
ENSMUST00000196221.1	9	6	0	0
ENSMUST00000179664.1	11	8	0	0
ENSMUST00000178537.1	12	9	0	0
ENSMUST00000178862.1	14	11	0	0
ENSMUST00000179520.1	11	8	0	0
ENSMUST00000179883.1	16	5	0	0
ENSMUST00000195858.1	10	7	0	0
ENSMUST00000179932.1	12	9	0	0

==> SRR14016363.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR14016363 completed mapping pipeline successfully
