Starting /dee2/code/volunteer_pipeline.sh SRR14016375
    current disk space = 318803764
    free memory = 263185740 
SRR14016375 SRAfilesize
fa6109dac9fbbf12a66cf3d026b4507b  SRR14016375.sra
SRR14016375.sra file validated
SRR14016375 is paired end
SRR14016375 is conventional basespace
SRR14016375 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.85	37.0	37.0	37.0	37.0	37.0
2	35.648	37.0	37.0	37.0	37.0	37.0
3	35.828	37.0	37.0	37.0	37.0	37.0
4	35.9175	37.0	37.0	37.0	37.0	37.0
5	36.014	37.0	37.0	37.0	37.0	37.0
6	35.9395	37.0	37.0	37.0	37.0	37.0
7	35.9305	37.0	37.0	37.0	37.0	37.0
8	36.0025	37.0	37.0	37.0	37.0	37.0
9	35.831	37.0	37.0	37.0	37.0	37.0
10-14	35.921749999999996	37.0	37.0	37.0	37.0	37.0
15-19	35.90765	37.0	37.0	37.0	37.0	37.0
20-24	35.6864	37.0	37.0	37.0	37.0	37.0
25-29	35.4368	37.0	37.0	37.0	34.6	37.0
30-34	35.3311	37.0	37.0	37.0	37.0	37.0
35-39	35.05409999999999	37.0	37.0	37.0	27.4	37.0
40-44	35.1133	37.0	37.0	37.0	27.4	37.0
45-49	34.702650000000006	37.0	37.0	37.0	25.0	37.0
50-54	34.134299999999996	37.0	37.0	37.0	25.0	37.0
55-59	32.64784999999999	37.0	37.0	37.0	11.0	37.0
60-64	32.29265	37.0	37.0	37.0	11.0	37.0
65-69	31.8671	37.0	37.0	37.0	11.0	37.0
70-74	31.326999999999998	37.0	25.0	37.0	11.0	37.0
75-79	31.409499999999998	37.0	27.4	37.0	11.0	37.0
80-84	31.3524	37.0	25.0	37.0	11.0	37.0
85-89	30.538800000000002	37.0	25.0	37.0	11.0	37.0
90-94	30.091	37.0	25.0	37.0	11.0	37.0
95-99	29.742399999999996	37.0	25.0	37.0	11.0	37.0
100-104	29.539099999999998	37.0	25.0	37.0	11.0	37.0
105-109	29.324400000000004	37.0	19.4	37.0	11.0	37.0
110-114	28.9899	37.0	11.0	37.0	11.0	37.0
115-119	28.751600000000003	37.0	11.0	37.0	11.0	37.0
120-124	28.437849999999997	37.0	11.0	37.0	11.0	37.0
125-129	28.079250000000002	37.0	11.0	37.0	11.0	37.0
130-134	27.6878	37.0	11.0	37.0	11.0	37.0
135-139	27.45785	37.0	11.0	37.0	11.0	37.0
140-144	27.42885	37.0	11.0	37.0	11.0	37.0
145-149	27.74975	37.0	11.0	37.0	11.0	37.0
150	27.7855	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	4.0
21	16.0
22	62.0
23	114.0
24	214.0
25	285.0
26	257.0
27	234.0
28	198.0
29	157.0
30	153.0
31	120.0
32	125.0
33	146.0
34	181.0
35	510.0
36	1167.0
37	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.35	28.249999999999996	12.8	27.6
2	16.3	27.175	31.374999999999996	25.15
3	26.05	20.7	30.675	22.575
4	22.925	32.775	17.224999999999998	27.075
5	23.5	40.025	18.099999999999998	18.375
6	30.45	25.900000000000002	25.974999999999998	17.675
7	24.325	22.525000000000002	29.9	23.25
8	30.3	26.700000000000003	21.224999999999998	21.775
9	27.700000000000003	22.35	29.549999999999997	20.4
10-14	21.636081804090203	28.431421571078552	25.65128256412821	24.281214060703036
15-19	24.87124356217811	25.681284064203208	24.366218310915546	25.081254062703135
20-24	24.595	28.02	28.499999999999996	18.884999999999998
25-29	19.64	25.115	33.505	21.740000000000002
30-34	21.4	23.630000000000003	35.92	19.05
35-39	18.3	23.880000000000003	38.855000000000004	18.965
40-44	19.74	24.365000000000002	38.915	16.98
45-49	18.48092404620231	24.946247312365617	40.0170008500425	16.555827791389568
50-54	20.205000000000002	26.525	36.385	16.885
55-59	23.121156057802892	27.641382069103454	30.64653232661633	18.590929546477327
60-64	21.76108805440272	31.64158207910395	27.49137456872844	19.105955297764886
65-69	21.37	33.155	27.775	17.7
70-74	22.95	32.025	26.58	18.445
75-79	26.740000000000002	32.214999999999996	21.52	19.525000000000002
80-84	25.124999999999996	32.89	22.09	19.895
85-89	22.615	34.48	20.549999999999997	22.355
90-94	22.17	35.54	20.64	21.65
95-99	20.805	36.57	19.435	23.189999999999998
100-104	19.805	38.475	19.13	22.59
105-109	18.555	38.385000000000005	20.31	22.75
110-114	18.827531012404965	37.820128051220486	20.793317326930772	22.559023609443777
115-119	20.55911182236447	35.61712342468494	21.599319863972795	22.224444888977796
120-124	21.6882532379857	34.140121018152726	22.188328249237387	21.983297494624193
125-129	21.71325698854828	32.88493273991099	22.02330349552433	23.3785067760164
130-134	23.482348234823483	33.25832583258326	22.55225522552255	20.707070707070706
135-139	26.193929089363404	31.38970845626844	22.32834925238786	20.0880132019803
140-144	29.22146107305365	32.0266013300665	21.476073803690184	17.27586379318966
145-149	33.25498824823724	29.31939790968645	21.50822623393509	15.91738760814122
150	37.375	25.974999999999998	21.975	14.674999999999999
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	2.0
3	1.5
4	1.5
5	2.0
6	3.5
7	3.0
8	6.5
9	8.5
10	7.5
11	9.5
12	11.0
13	16.5
14	23.0
15	21.0
16	30.5
17	41.5
18	35.0
19	30.5
20	28.5
21	29.0
22	38.0
23	40.5
24	38.0
25	42.0
26	41.0
27	46.5
28	51.0
29	57.0
30	80.5
31	95.0
32	101.5
33	118.5
34	132.5
35	131.0
36	125.5
37	113.0
38	90.0
39	60.0
40	44.5
41	45.0
42	35.5
43	28.5
44	33.5
45	36.0
46	49.0
47	62.5
48	84.0
49	105.5
50	122.5
51	152.5
52	176.5
53	188.0
54	174.0
55	155.5
56	125.0
57	105.0
58	112.0
59	119.0
60	107.0
61	71.0
62	41.0
63	27.5
64	27.5
65	26.5
66	14.0
67	3.0
68	1.0
69	2.0
70	2.0
71	0.5
72	0.0
73	1.0
74	2.0
75	1.0
76	1.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	1.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.005
15-19	0.005
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.005
50-54	0.0
55-59	0.005
60-64	0.005
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.04
115-119	0.02
120-124	0.015
125-129	0.015
130-134	0.01
135-139	0.015
140-144	0.005
145-149	0.015
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02127659574468	33.85
2	12.978723404255318	12.2
3	5.212765957446808	7.35
4	2.925531914893617	5.5
5	1.1170212765957448	2.625
6	1.7553191489361704	4.95
7	1.0638297872340425	3.5000000000000004
8	0.7446808510638299	2.8000000000000003
9	0.37234042553191493	1.575
>10	1.5957446808510638	9.9
>50	0.10638297872340426	3.45
>100	0.10638297872340426	12.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	330	8.25	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	162	4.05	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	77	1.925	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	61	1.525	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.65	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	19	0.475	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.44999999999999996	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	17	0.42500000000000004	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	17	0.42500000000000004	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.42500000000000004	No Hit
GTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGA	16	0.4	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	16	0.4	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	15	0.375	No Hit
CAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGC	14	0.35000000000000003	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	14	0.35000000000000003	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	13	0.325	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	13	0.325	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	12	0.3	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	12	0.3	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	12	0.3	No Hit
GAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCC	11	0.27499999999999997	No Hit
TCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCC	11	0.27499999999999997	No Hit
CTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATG	11	0.27499999999999997	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	11	0.27499999999999997	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	11	0.27499999999999997	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	10	0.25	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	10	0.25	No Hit
GGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCC	10	0.25	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	10	0.25	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	10	0.25	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	10	0.25	No Hit
CAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAA	10	0.25	No Hit
AGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGT	10	0.25	No Hit
GCCAGAGGCTGGTGATGGGACCACCGAGATCTCACCCACTGGTGCTGCTG	9	0.22499999999999998	No Hit
GTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAA	9	0.22499999999999998	No Hit
AGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATT	9	0.22499999999999998	No Hit
AAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTT	9	0.22499999999999998	No Hit
ATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTC	9	0.22499999999999998	No Hit
CCGCCTGGCTTCTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCA	9	0.22499999999999998	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	9	0.22499999999999998	No Hit
GCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAA	8	0.2	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	8	0.2	No Hit
TGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGG	8	0.2	No Hit
CTCACGGTAGGCCCGCTTCTCCACCAGCTTCACTTTGTATTTGCGCTTGA	8	0.2	No Hit
GTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAA	8	0.2	No Hit
ACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAG	8	0.2	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	8	0.2	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
TTTGCGCTTGAACTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCT	8	0.2	No Hit
GTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTG	8	0.2	No Hit
AGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTG	8	0.2	No Hit
GCCAAGAAGCTCAGGCGAAAGGAGGAGTTATGGGAGAAACTGGCAAAGCA	8	0.2	No Hit
CTTTCTCACGCTGCAGCTCCACCTCATGGGCCTCTCGAAGCAGGGCCTGG	8	0.2	No Hit
AATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTG	8	0.2	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	7	0.17500000000000002	No Hit
ATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCTC	7	0.17500000000000002	No Hit
GTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTGGCT	7	0.17500000000000002	No Hit
AAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGAT	7	0.17500000000000002	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCTAAAAAAAAAAAAAA	7	0.17500000000000002	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	7	0.17500000000000002	No Hit
TCTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCT	7	0.17500000000000002	No Hit
CTTGTCTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTT	7	0.17500000000000002	No Hit
GTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTG	7	0.17500000000000002	No Hit
ATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTG	7	0.17500000000000002	No Hit
AAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTG	7	0.17500000000000002	No Hit
CTCAGGACACTGAAGCCAGAAGGTCACATTCTCCGAGACAGGTTCAAGAG	7	0.17500000000000002	No Hit
CAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTCTATGAC	7	0.17500000000000002	No Hit
CGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATA	7	0.17500000000000002	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	7	0.17500000000000002	No Hit
GATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGC	7	0.17500000000000002	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	7	0.17500000000000002	No Hit
ATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGT	7	0.17500000000000002	No Hit
ATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTG	7	0.17500000000000002	No Hit
CCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.15	No Hit
AAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCG	6	0.15	No Hit
GGAGGCTCCTGCAGGAATCACCTCCACTGCAGGCACCTGGGAAGGCTTGA	6	0.15	No Hit
GGAATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTG	6	0.15	No Hit
ACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGG	6	0.15	No Hit
GAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTCC	6	0.15	No Hit
CGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTTAA	6	0.15	No Hit
ATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGCC	6	0.15	No Hit
GCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGAT	6	0.15	No Hit
GTCCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGA	6	0.15	No Hit
CTGCAGGAATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGG	6	0.15	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	6	0.15	No Hit
CTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTG	6	0.15	No Hit
GTGAGGGCCTGCTGGAGGAGTCTGATGGTGAGGATGAGCATGAGGCAGGC	6	0.15	No Hit
GCGACAGCTGGCCCTGCCCACCTCAGAGCAAGCTGCCACCCAGGAGTCCG	6	0.15	No Hit
CCTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTG	6	0.15	No Hit
GTTCCAGAAAAAATGCATCCTGACCAATCAAAGGCGTGTCCAGAGGGTTG	6	0.15	No Hit
TTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CCCGACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGAC	6	0.15	No Hit
ATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGG	6	0.15	No Hit
GCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCG	6	0.15	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTAA	6	0.15	No Hit
GTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGA	6	0.15	No Hit
GAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAG	6	0.15	No Hit
GCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAG	6	0.15	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	6	0.15	No Hit
GAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAG	6	0.15	No Hit
GGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCAGGCACCTGGGAA	6	0.15	No Hit
CTGGACACGCCTTTGATTGGTCAGGATGCATTTTTTCTGGAACAGACCAA	6	0.15	No Hit
GAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAAT	6	0.15	No Hit
ATTCAAGAGAAAAGAACCAAGAAAGAAGAGGACCTTGGTCCAGAAGAAGT	6	0.15	No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGG	6	0.15	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	5	0.125	No Hit
TGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAG	5	0.125	No Hit
ACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAA	5	0.125	No Hit
ATTCTCCGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCC	5	0.125	No Hit
ACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTC	5	0.125	No Hit
TGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGC	5	0.125	No Hit
CTTCTTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATC	5	0.125	No Hit
GACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATC	5	0.125	No Hit
CTCCCATAACTCCTCCTTTCGCCTGAGCTTCTTGGCATTAGGGACCTGAT	5	0.125	No Hit
TTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCG	5	0.125	No Hit
AGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTTAAAG	5	0.125	No Hit
GTCCCTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCG	5	0.125	No Hit
CTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACG	5	0.125	No Hit
CGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAA	5	0.125	No Hit
ACTGAAGCCAGAAGGTCACATTCTCCGAGACAGGTTCAAGAGCTTCCAGA	5	0.125	No Hit
GGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAACAATTT	5	0.125	No Hit
AAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCTCCTA	5	0.125	No Hit
CCGACATCTGCACAGCTACAACTGAATCTCACGGTAGGCCCGCTTCTCCA	5	0.125	No Hit
GCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTC	5	0.125	No Hit
TCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCAGG	5	0.125	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.1375	0.0	0.0	0.0	0.0
32-33	0.2	0.0	0.0	0.0	0.0
34-35	0.275	0.0	0.0	0.0	0.0
36-37	0.3625	0.0	0.0	0.0	0.0
38-39	0.4125	0.0	0.0	0.0	0.0
40-41	0.475	0.0	0.0	0.0	0.0
42-43	0.625	0.0	0.0	0.0	0.0
44-45	0.875	0.0	0.0	0.0	0.0
46-47	1.075	0.0	0.0	0.0	0.0
48-49	1.25	0.0	0.0	0.0	0.0
50-51	1.475	0.0	0.0	0.0	0.0
52-53	1.7625	0.0	0.0	0.0	0.0
54-55	2.175	0.0	0.0	0.0	0.0
56-57	2.6125	0.0	0.0	0.0	0.0
58-59	3.2375	0.0	0.0	0.0	0.0
60-61	3.7874999999999996	0.0	0.0	0.0	0.0
62-63	4.487500000000001	0.0	0.0	0.0	0.0
64-65	5.625	0.0	0.0	0.0	0.0
66-67	6.8625	0.0	0.0	0.0	0.0
68-69	8.0625	0.0	0.0	0.0	0.0
70-71	8.9625	0.0	0.0	0.0	0.0
72-73	10.225000000000001	0.0	0.0	0.0	0.0
74-75	11.525	0.0	0.0	0.0	0.0
76-77	13.037500000000001	0.0	0.0	0.0	0.0
78-79	14.4875	0.0	0.0	0.0	0.0
80-81	15.7875	0.0	0.0	0.0	0.0
82-83	17.262500000000003	0.0	0.0	0.0	0.0
84-85	19.075	0.0	0.0	0.0	0.0
86-87	20.7625	0.0	0.0	0.0	0.0
88-89	21.987499999999997	0.0	0.0	0.0	0.0
90-91	23.4625	0.0	0.0	0.0	0.0
92-93	25.4625	0.0	0.0	0.0	0.0
94-95	27.575000000000003	0.0	0.0	0.0	0.0
96-97	29.9125	0.0	0.0	0.0	0.0
98-99	32.15	0.0	0.0	0.0	0.0
100-101	33.2375	0.0	0.0	0.0	0.0
102-103	34.075	0.0	0.0	0.0	0.0
104-105	34.8125	0.0	0.0	0.0	0.0
106-107	35.575	0.0	0.0	0.0	0.0
108-109	36.35	0.0	0.0	0.0	0.0
110-111	37.2	0.0	0.0	0.0	0.0
112-113	37.8	0.0	0.0	0.0	0.0
114-115	38.425	0.0	0.0	0.0	0.0
116-117	39.1875	0.0	0.0	0.0	0.0
118-119	39.787499999999994	0.0	0.0	0.0	0.0
120-121	40.425	0.0	0.0	0.0	0.0
122-123	40.8125	0.0	0.0	0.0	0.0
124-125	41.0875	0.0	0.0	0.0	0.0
126-127	41.5625	0.0	0.0	0.0	0.0
128-129	41.925	0.0	0.0	0.0	0.0
130-131	42.3375	0.0	0.0	0.0	0.0
132-133	42.55	0.0	0.0	0.0	0.0
134-135	43.025000000000006	0.0	0.0	0.0	0.0
136-137	43.6	0.0	0.0	0.0	0.0
138	43.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATT	20	1.9452218E-6	144.0	4
GCTAATC	20	1.9452218E-6	144.0	1
CTAATCA	20	1.9452218E-6	144.0	2
TAATCAT	20	1.9452218E-6	144.0	3
TCATTGC	20	1.9452218E-6	144.0	6
ATCATTG	25	5.904236E-6	115.2	5
CATTGCA	30	1.46121765E-5	96.0	7
TTGCAAG	35	3.1411873E-5	82.28571	9
ATTGCAA	40	6.0911432E-5	72.0	8
AGCAGTG	65	1.1285738E-7	66.46153	2
AAGCAGT	65	1.1285738E-7	66.46153	1
AGTGGTA	70	1.8854553E-7	61.714283	5
CAGTGGT	70	1.8854553E-7	61.714283	4
GCAGTGG	80	4.748381E-7	54.0	3
TGGTATC	90	1.0710228E-6	48.0	7
GTATCAA	95	1.5551013E-6	45.473682	9
GTGGTAT	95	1.5551013E-6	45.473682	6
GGTATCA	95	1.5551013E-6	45.473682	8
TATCAAC	90	0.0033271667	32.0	5
AACGCAG	95	5.470836E-4	30.315788	9
>>END_MODULE
SRR14016375 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016375_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.83475	37.0	37.0	37.0	25.0	37.0
2	34.70925	37.0	37.0	37.0	25.0	37.0
3	35.201	37.0	37.0	37.0	25.0	37.0
4	35.554	37.0	37.0	37.0	37.0	37.0
5	35.694	37.0	37.0	37.0	37.0	37.0
6	35.6035	37.0	37.0	37.0	37.0	37.0
7	35.63	37.0	37.0	37.0	37.0	37.0
8	35.734	37.0	37.0	37.0	37.0	37.0
9	35.662	37.0	37.0	37.0	37.0	37.0
10-14	35.6055	37.0	37.0	37.0	37.0	37.0
15-19	35.66074999999999	37.0	37.0	37.0	37.0	37.0
20-24	35.563599999999994	37.0	37.0	37.0	37.0	37.0
25-29	35.394349999999996	37.0	37.0	37.0	37.0	37.0
30-34	35.41095	37.0	37.0	37.0	37.0	37.0
35-39	34.9568	37.0	37.0	37.0	25.0	37.0
40-44	34.8148	37.0	37.0	37.0	25.0	37.0
45-49	34.437	37.0	37.0	37.0	25.0	37.0
50-54	33.8176	37.0	37.0	37.0	22.2	37.0
55-59	32.36424999999999	37.0	37.0	37.0	11.0	37.0
60-64	32.186400000000006	37.0	37.0	37.0	11.0	37.0
65-69	31.5936	37.0	29.8	37.0	11.0	37.0
70-74	30.945349999999998	37.0	25.0	37.0	11.0	37.0
75-79	31.003500000000003	37.0	25.0	37.0	11.0	37.0
80-84	30.703999999999997	37.0	25.0	37.0	11.0	37.0
85-89	29.9822	37.0	25.0	37.0	11.0	37.0
90-94	29.889400000000002	37.0	25.0	37.0	11.0	37.0
95-99	29.361400000000003	37.0	16.6	37.0	11.0	37.0
100-104	29.0824	37.0	11.0	37.0	11.0	37.0
105-109	28.8995	37.0	11.0	37.0	11.0	37.0
110-114	28.555200000000003	37.0	11.0	37.0	11.0	37.0
115-119	28.3928	37.0	11.0	37.0	11.0	37.0
120-124	28.2169	37.0	11.0	37.0	11.0	37.0
125-129	27.771800000000002	37.0	11.0	37.0	11.0	37.0
130-134	27.7575	37.0	11.0	37.0	11.0	37.0
135-139	27.591199999999997	37.0	11.0	37.0	11.0	37.0
140-144	27.439999999999998	37.0	11.0	37.0	11.0	37.0
145-149	27.452999999999996	37.0	11.0	37.0	11.0	37.0
150	27.605	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	5.0
19	2.0
20	9.0
21	43.0
22	87.0
23	161.0
24	208.0
25	305.0
26	265.0
27	176.0
28	174.0
29	121.0
30	121.0
31	114.0
32	120.0
33	176.0
34	303.0
35	646.0
36	929.0
37	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.117764971185167	28.063142069656728	13.05437233775996	28.764720621398144
2	15.928982245561391	26.281570392598148	29.75743935983996	28.032008002000502
3	24.8	21.7	30.825000000000003	22.675
4	19.875	32.675	18.425	29.025000000000002
5	21.85	40.45	19.775000000000002	17.925
6	28.525	26.674999999999997	26.625	18.175
7	23.75	21.575	28.875	25.8
8	28.925	28.199999999999996	21.175	21.7
9	26.525	23.025000000000002	30.975	19.475
10-14	21.555	28.544999999999998	25.445	24.455
15-19	24.411220561028053	25.681284064203208	25.501275063753187	24.40622031101555
20-24	24.4	26.77	29.5	19.33
25-29	19.255962798139905	25.131256562828142	33.426671333566674	22.186109305465273
30-34	21.411070553527676	25.146257312865643	34.67173358667933	18.770938546927347
35-39	17.560000000000002	24.529999999999998	38.845	19.064999999999998
40-44	19.869999999999997	24.64	38.12	17.37
45-49	19.145	24.72	39.795	16.34
50-54	19.525000000000002	26.255	36.730000000000004	17.49
55-59	23.176158807940396	27.076353817690883	31.90659532976649	17.84089204460223
60-64	22.27	30.205	29.095	18.43
65-69	22.725	31.064999999999998	29.09	17.119999999999997
70-74	24.586229311465573	30.69653482674134	27.246362318115906	17.470873543677186
75-79	27.71	30.885	23.39	18.015
80-84	28.28282828282828	30.71807180718072	24.007400740074008	16.99169916991699
85-89	27.48	32.795	21.81	17.915
90-94	28.82	33.650000000000006	21.490000000000002	16.04
95-99	29.185	35.0	20.18	15.634999999999998
100-104	28.23	36.980000000000004	19.84	14.95
105-109	29.43	35.635	20.82	14.115
110-114	29.095	35.975	21.135	13.794999999999998
115-119	31.055	36.305	20.119999999999997	12.520000000000001
120-124	31.485000000000003	35.644999999999996	20.125	12.745000000000001
125-129	32.11	34.385	20.455000000000002	13.05
130-134	33.32	33.545	20.0	13.135
135-139	36.3	32.775	18.634999999999998	12.29
140-144	38.745000000000005	32.66	17.635	10.96
145-149	40.86	31.47	18.099999999999998	9.569999999999999
150	44.375	29.9	17.599999999999998	8.125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.5
3	3.0
4	5.5
5	6.0
6	4.0
7	5.5
8	7.5
9	9.0
10	11.0
11	11.5
12	14.5
13	17.5
14	17.5
15	30.0
16	36.5
17	33.5
18	33.5
19	28.5
20	31.5
21	32.5
22	28.5
23	32.0
24	39.5
25	48.0
26	51.0
27	50.5
28	57.0
29	75.0
30	92.5
31	93.5
32	88.5
33	100.5
34	117.0
35	111.5
36	102.5
37	91.0
38	76.5
39	60.0
40	46.0
41	41.0
42	39.5
43	35.5
44	31.5
45	45.5
46	55.0
47	70.0
48	86.0
49	95.0
50	134.5
51	165.5
52	173.0
53	191.0
54	169.0
55	145.5
56	127.5
57	103.0
58	113.5
59	117.5
60	101.5
61	71.0
62	48.0
63	32.5
64	32.5
65	32.0
66	13.5
67	5.5
68	4.5
69	3.5
70	1.5
71	0.5
72	0.5
73	0.5
74	2.5
75	2.0
76	1.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22499999999999998
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.005
20-24	0.0
25-29	0.005
30-34	0.005
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.005
60-64	0.0
65-69	0.0
70-74	0.005
75-79	0.0
80-84	0.01
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.24999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.58706467661692	36.475
2	13.233830845771145	13.3
3	5.6716417910447765	8.55
4	2.3880597014925375	4.8
5	1.5920398009950247	4.0
6	1.3432835820895521	4.05
7	0.7462686567164178	2.625
8	0.5970149253731344	2.4
9	0.3482587064676617	1.575
>10	1.2935323383084576	8.525
>50	0.09950248756218905	2.65
>100	0.09950248756218905	11.05
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	265	6.625	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	177	4.425	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	55	1.375	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	51	1.275	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.775	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	24	0.6	No Hit
GTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGA	17	0.42500000000000004	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	16	0.4	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.375	No Hit
ATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGCC	14	0.35000000000000003	No Hit
GTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAA	14	0.35000000000000003	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.35000000000000003	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	13	0.325	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	13	0.325	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	12	0.3	No Hit
ATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTG	12	0.3	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	11	0.27499999999999997	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	11	0.27499999999999997	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	11	0.27499999999999997	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	11	0.27499999999999997	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	11	0.27499999999999997	No Hit
CCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10	0.25	No Hit
ATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCTC	10	0.25	No Hit
CATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCT	10	0.25	No Hit
CTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGG	10	0.25	No Hit
CGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCA	10	0.25	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	10	0.25	No Hit
CAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGC	10	0.25	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.25	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	9	0.22499999999999998	No Hit
ACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGG	9	0.22499999999999998	No Hit
GCCAGAGGCTGGTGATGGGACCACCGAGATCTCACCCACTGGTGCTGCTG	9	0.22499999999999998	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
GAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCC	9	0.22499999999999998	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	9	0.22499999999999998	No Hit
ATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGT	9	0.22499999999999998	No Hit
GGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCC	8	0.2	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	8	0.2	No Hit
CTTGAACTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGC	8	0.2	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	8	0.2	No Hit
AATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTG	8	0.2	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	8	0.2	No Hit
GGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGCCTTTCTTCTTGGTCTG	8	0.2	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	8	0.2	No Hit
GCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTC	8	0.2	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	8	0.2	No Hit
TTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCT	8	0.2	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	8	0.2	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	7	0.17500000000000002	No Hit
GTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTGGCT	7	0.17500000000000002	No Hit
TGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAG	7	0.17500000000000002	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	7	0.17500000000000002	No Hit
GTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAA	7	0.17500000000000002	No Hit
ATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
ATACAATTTCAAACAAATGCCAACTGGAAGACAATGTCCAAAAACTCTGA	7	0.17500000000000002	No Hit
GACAGCTGGCCCTGCCCACCTCAGAGCAAGCTGCCACCCAGGAGTCCGTG	7	0.17500000000000002	No Hit
GTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGA	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGTCCTTTA	7	0.17500000000000002	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	7	0.17500000000000002	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	7	0.17500000000000002	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	7	0.17500000000000002	No Hit
CTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCC	7	0.17500000000000002	No Hit
AGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGT	7	0.17500000000000002	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAGA	6	0.15	No Hit
CTCAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTC	6	0.15	No Hit
CTGCAGGAATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGG	6	0.15	No Hit
GTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTG	6	0.15	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	6	0.15	No Hit
CTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGA	6	0.15	No Hit
TGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGAT	6	0.15	No Hit
CCCAAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAAC	6	0.15	No Hit
GTTCCAGAAAAAATGCATCCTGACCAATCAAAGGCGTGTCCAGAGGGTTG	6	0.15	No Hit
ATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTC	6	0.15	No Hit
CTCCGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCG	6	0.15	No Hit
GTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCA	6	0.15	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	6	0.15	No Hit
CTCTTCTTTCTTGGTTCTTTTCTCTTGAATCCTGTGTCCACGAAGAAGAG	6	0.15	No Hit
CTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGC	6	0.15	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTAA	6	0.15	No Hit
CTCACGCTGCAGCTCCACCTCATGGGCCTCTCGAAGCAGGGCCTGGTGAT	6	0.15	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTCGAGCCGCGCT	6	0.15	No Hit
CCGCCTGGCTTCTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCA	6	0.15	No Hit
GTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGT	6	0.15	No Hit
CCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGCCTTTCTTC	6	0.15	No Hit
CAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAA	6	0.15	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	6	0.15	No Hit
CTGGCAGAGGCTGACAAGCCCCGAAGGCTGGGACGGCTCAAGTACCAGGC	6	0.15	No Hit
AGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGA	6	0.15	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	5	0.125	No Hit
CCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTC	5	0.125	No Hit
AAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTGG	5	0.125	No Hit
ACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAA	5	0.125	No Hit
ATTCTCCGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCC	5	0.125	No Hit
GCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGAT	5	0.125	No Hit
GTCCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGA	5	0.125	No Hit
GTCCTGGGGCCCAGGTTTGGCCTTTGGTGTGGGAGGGCTAAGGAGTCGGG	5	0.125	No Hit
CCACCGAGGTCGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTC	5	0.125	No Hit
CCCAGACAACCCTCTGGACACGCCTTTGATTGGTCAGGATGCATTTTTTC	5	0.125	No Hit
CTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTG	5	0.125	No Hit
CTTCTTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATC	5	0.125	No Hit
CAAGGGCAAGGTCAACCCGTAAGGGTTTCTGGAGACGCTGTGACTTCTTC	5	0.125	No Hit
TCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTA	5	0.125	No Hit
ACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGATACCACTG	5	0.125	No Hit
CTTTATTGAGGGGCGGGGCTCCGACATCTGCACAGCTACAACTGAATCTC	5	0.125	No Hit
GACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATC	5	0.125	No Hit
GAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGG	5	0.125	No Hit
GATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTT	5	0.125	No Hit
CTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGCTCTTGA	5	0.125	No Hit
GGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCC	5	0.125	No Hit
CCGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAG	5	0.125	No Hit
AAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCT	5	0.125	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CCACCTCATGGGCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGG	5	0.125	No Hit
TATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGA	5	0.125	No Hit
ATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCT	5	0.125	No Hit
ATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAA	5	0.125	No Hit
CTGCAGTGGAGGTGATTCCTGCAGGAGCCTCCTACAACCCAACCTTTGAA	5	0.125	No Hit
GCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGCTCTTGAACCT	5	0.125	No Hit
CCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTCTATGA	5	0.125	No Hit
TTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.1375	0.0	0.0	0.0	0.0
32-33	0.2	0.0	0.0	0.0	0.0
34-35	0.275	0.0	0.0	0.0	0.0
36-37	0.3625	0.0	0.0	0.0	0.0
38-39	0.4125	0.0	0.0	0.0	0.0
40-41	0.475	0.0	0.0	0.0	0.0
42-43	0.625	0.0	0.0	0.0	0.0
44-45	0.875	0.0	0.0	0.0	0.0
46-47	1.075	0.0	0.0	0.0	0.0
48-49	1.25	0.0	0.0	0.0	0.0
50-51	1.4375	0.0	0.0	0.0	0.0
52-53	1.7	0.0	0.0	0.0	0.0
54-55	2.1	0.0	0.0	0.0	0.0
56-57	2.5125	0.0	0.0	0.0	0.0
58-59	3.125	0.0	0.0	0.0	0.0
60-61	3.6875	0.0	0.0	0.0	0.0
62-63	4.4125	0.0	0.0	0.0	0.0
64-65	5.574999999999999	0.0	0.0	0.0	0.0
66-67	6.8125	0.0	0.0	0.0	0.0
68-69	8.0125	0.0	0.0	0.0	0.0
70-71	8.925	0.0	0.0	0.0	0.0
72-73	10.1375	0.0	0.0	0.0	0.0
74-75	11.525	0.0	0.0	0.0	0.0
76-77	12.912500000000001	0.0	0.0	0.0	0.0
78-79	14.2625	0.0	0.0	0.0	0.0
80-81	15.45	0.0	0.0	0.0	0.0
82-83	16.875	0.0	0.0	0.0	0.0
84-85	18.5125	0.0	0.0	0.0	0.0
86-87	20.1875	0.0	0.0	0.0	0.0
88-89	21.475	0.0	0.0	0.0	0.0
90-91	22.875	0.0	0.0	0.0	0.0
92-93	24.825	0.0	0.0	0.0	0.0
94-95	26.8625	0.0	0.0	0.0	0.0
96-97	29.112499999999997	0.0	0.0	0.0	0.0
98-99	31.2125	0.0	0.0	0.0	0.0
100-101	32.325	0.0	0.0	0.0	0.0
102-103	33.2	0.0	0.0	0.0	0.0
104-105	33.8875	0.0	0.0	0.0	0.0
106-107	34.575	0.0	0.0	0.0	0.0
108-109	35.3125	0.0	0.0	0.0	0.0
110-111	36.1125	0.0	0.0	0.0	0.0
112-113	36.6875	0.0	0.0	0.0	0.0
114-115	37.3125	0.0	0.0	0.0	0.0
116-117	38.0125	0.0	0.0	0.0	0.0
118-119	38.575	0.0	0.0	0.0	0.0
120-121	39.1625	0.0	0.0	0.0	0.0
122-123	39.525000000000006	0.0	0.0	0.0	0.0
124-125	39.7	0.0	0.0	0.0	0.0
126-127	40.125	0.0	0.0	0.0	0.0
128-129	40.5375	0.0	0.0	0.0	0.0
130-131	40.9625	0.0	0.0	0.0	0.0
132-133	41.175	0.0	0.0	0.0	0.0
134-135	41.6875	0.0	0.0	0.0	0.0
136-137	42.1875	0.0	0.0	0.0	0.0
138	42.5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	25	3.201967E-8	144.0	1
CTAATCA	30	5.2386895E-10	144.0	2
TAATCAT	30	5.2386895E-10	144.0	3
TCATTGC	30	5.2386895E-10	144.0	6
TTGCAAG	35	1.5315891E-9	123.428566	9
AATCATT	35	1.5315891E-9	123.428566	4
ATTGCAA	40	3.8780854E-9	108.0	8
ATCATTG	40	3.8780854E-9	108.0	5
CATTGCA	45	8.794814E-9	96.0	7
AAGCAGT	65	1.1285738E-7	66.46153	1
AGCAGTG	75	3.0391493E-7	57.6	2
TGGTATC	80	4.748381E-7	54.0	7
AGTGGTA	80	4.748381E-7	54.0	5
GTGGTAT	80	4.748381E-7	54.0	6
CAGTGGT	80	4.748381E-7	54.0	4
GTATCAA	85	7.218405E-7	50.82353	9
GCAGTGG	90	1.0710228E-6	48.0	3
GGTATCA	90	1.0710228E-6	48.0	8
GTACATG	20	0.006139246	28.8	30-34
AGTACAT	20	0.006139246	28.8	30-34
>>END_MODULE
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001264 spots for SRR14016375.sra
Written 1001264 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
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Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
Read 1001229 spots for SRR14016375.sra
Written 1001229 spots for SRR14016375.sra
SRR ids: ['SRR14016375.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_78yd7zwm
SRR14016375.sra spots: 64078691
blocks: [[1, 1001229], [1001230, 2002458], [2002459, 3003687], [3003688, 4004916], [4004917, 5006145], [5006146, 6007374], [6007375, 7008603], [7008604, 8009832], [8009833, 9011061], [9011062, 10012290], [10012291, 11013519], [11013520, 12014748], [12014749, 13015977], [13015978, 14017206], [14017207, 15018435], [15018436, 16019664], [16019665, 17020893], [17020894, 18022122], [18022123, 19023351], [19023352, 20024580], [20024581, 21025809], [21025810, 22027038], [22027039, 23028267], [23028268, 24029496], [24029497, 25030725], [25030726, 26031954], [26031955, 27033183], [27033184, 28034412], [28034413, 29035641], [29035642, 30036870], [30036871, 31038099], [31038100, 32039328], [32039329, 33040557], [33040558, 34041786], [34041787, 35043015], [35043016, 36044244], [36044245, 37045473], [37045474, 38046702], [38046703, 39047931], [39047932, 40049160], [40049161, 41050389], [41050390, 42051618], [42051619, 43052847], [43052848, 44054076], [44054077, 45055305], [45055306, 46056534], [46056535, 47057763], [47057764, 48058992], [48058993, 49060221], [49060222, 50061450], [50061451, 51062679], [51062680, 52063908], [52063909, 53065137], [53065138, 54066366], [54066367, 55067595], [55067596, 56068824], [56068825, 57070053], [57070054, 58071282], [58071283, 59072511], [59072512, 60073740], [60073741, 61074969], [61074970, 62076198], [62076199, 63077427], [63077428, 64078691]]
SRR14016375 file size 21629892
SRR14016375 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016375 SRR14016375_1.fastq SRR14016375_2.fastq
Input file:	SRR14016375_1.fastq
Paired file:	SRR14016375_2.fastq
trimmed:	SRR14016375-trimmed-pair1.fastq, SRR14016375-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 05:45:42 2023 >> started

Thu May 11 05:48:08 2023 >> done (146.471s)
64078691 read pairs processed; of these:
    1505 ( 0.00%) short read pairs filtered out after trimming by size control
   14949 ( 0.02%) empty read pairs filtered out after trimming by size control
64062237 (99.97%) read pairs available; of these:
30082366 (46.96%) trimmed read pairs available after processing
33979871 (53.04%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     190	  0.00%
 19	     179	  0.00%
 20	     307	  0.00%
 21	     591	  0.00%
 22	    1653	  0.00%
 23	    1995	  0.00%
 24	    2140	  0.00%
 25	    2859	  0.00%
 26	    3900	  0.01%
 27	    5599	  0.01%
 28	    8242	  0.01%
 29	   11733	  0.02%
 30	   16357	  0.03%
 31	   22154	  0.03%
 32	   27278	  0.04%
 33	   27984	  0.04%
 34	   32238	  0.05%
 35	   31892	  0.05%
 36	   32872	  0.05%
 37	   37060	  0.06%
 38	   39397	  0.06%
 39	   43929	  0.07%
 40	   45116	  0.07%
 41	   48832	  0.08%
 42	   56379	  0.09%
 43	   57254	  0.09%
 44	   61682	  0.10%
 45	   64609	  0.10%
 46	   68353	  0.11%
 47	   72942	  0.11%
 48	   76954	  0.12%
 49	   83093	  0.13%
 50	   88330	  0.14%
 51	   93947	  0.15%
 52	  100511	  0.16%
 53	  106158	  0.17%
 54	  113703	  0.18%
 55	  119289	  0.19%
 56	  123899	  0.19%
 57	  129364	  0.20%
 58	  134429	  0.21%
 59	  139854	  0.22%
 60	  145528	  0.23%
 61	  151421	  0.24%
 62	  158582	  0.25%
 63	  166369	  0.26%
 64	  174564	  0.27%
 65	  180477	  0.28%
 66	  187862	  0.29%
 67	  193881	  0.30%
 68	  199278	  0.31%
 69	  205788	  0.32%
 70	  213456	  0.33%
 71	  220306	  0.34%
 72	  227940	  0.36%
 73	  236906	  0.37%
 74	  244439	  0.38%
 75	  249814	  0.39%
 76	  257775	  0.40%
 77	  262107	  0.41%
 78	  267317	  0.42%
 79	  271763	  0.42%
 80	  274863	  0.43%
 81	  281987	  0.44%
 82	  288175	  0.45%
 83	  294359	  0.46%
 84	  299351	  0.47%
 85	  305388	  0.48%
 86	  309560	  0.48%
 87	  312753	  0.49%
 88	  318241	  0.50%
 89	  320213	  0.50%
 90	  323277	  0.50%
 91	  328480	  0.51%
 92	  331091	  0.52%
 93	  333916	  0.52%
 94	  339132	  0.53%
 95	  341462	  0.53%
 96	  344898	  0.54%
 97	  347641	  0.54%
 98	  348847	  0.54%
 99	  350472	  0.55%
100	  353079	  0.55%
101	  352090	  0.55%
102	  354845	  0.55%
103	  358098	  0.56%
104	  357551	  0.56%
105	  359589	  0.56%
106	  360220	  0.56%
107	  360426	  0.56%
108	  359479	  0.56%
109	  359722	  0.56%
110	  359883	  0.56%
111	  360563	  0.56%
112	  360345	  0.56%
113	  360665	  0.56%
114	  362209	  0.57%
115	  362050	  0.57%
116	  361468	  0.56%
117	  359648	  0.56%
118	  360550	  0.56%
119	  357810	  0.56%
120	  356532	  0.56%
121	  356781	  0.56%
122	  354359	  0.55%
123	  354448	  0.55%
124	  356429	  0.56%
125	  353436	  0.55%
126	  351824	  0.55%
127	  352387	  0.55%
128	  350139	  0.55%
129	  346828	  0.54%
130	  347121	  0.54%
131	  343753	  0.54%
132	  341421	  0.53%
133	  343375	  0.54%
134	  339561	  0.53%
135	  339093	  0.53%
136	  337219	  0.53%
137	  336166	  0.52%
138	  333646	  0.52%
139	  330844	  0.52%
140	  328494	  0.51%
141	  327507	  0.51%
142	  325767	  0.51%
143	  324634	  0.51%
144	  317760	  0.50%
145	  317373	  0.50%
146	  316604	  0.49%
147	  315669	  0.49%
148	  317204	  0.50%
149	  340776	  0.53%
150	33979871	 53.04%
64062237 reads passed initial QC


criterion=sequence-density
sequence-density=3.45
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=35
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=29
fanout-score=32.03
fanout-score-rank=1
prefix-density=1.79
prefix-fanout=1.0
sequence=TACATGGGGCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTCGTGCTCCGCGCTCGCTGCAGCCACCTTCGCCGCCCACCGCCTCCTCCAGCGCGGACTCCGGCAGCTCTCTCGCCAGAGTCCTCGAACTCGACTTTTAATTCCCTCACTCGCGGCATCGGACCACCGGCGTGCCCCACCATGTCAGACGCGGCAGTGGATACCAGCTCCGAGATCACCACCAAGGACTTGAAGGAGAAGAAGGAAGTTGTGGAGGAGGCAGAGAATGGAAGAGATGCACCTGCCAATGGGAACGCTCAAAATGAGGAAAATGGGGAGCAGGAGG


criterion=sequence-density
sequence-density=3.44
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=37
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=43.04
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=5.8
sequence=CCTCCTGCTCCCCATTTTCCTCATTTTGAGCGTTCCCATTGGCAGGTGCATCTCTTCCATTCTCTGCCTCCTCCACAACTTCCTTCTTCTCCTTCAAGTCCTTGGTGGTGATCTCGGAGCTGGTATCCACTGCCGCGTCTGACATGGTGGGGCACGCCGGTGGTCCGATGCCGCGAGTGAGGGAATTAAAAGTCGAGTTCGAGGACTCTGGCGAGAGAGCTGCCGGAGTCCGCGCTGGAGGAGGCGGTGGGCGGCGAAGGTGGCTGCAGCGAGCGCGGAGCACGACCCAGGAACAATGCAAAGATGGCTTTTCGGAGCAGCCAGTGGGGCCCCATGTA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG -y GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG -o SRR14016375 SRR14016375_1.fastq SRR14016375_2.fastq
Input file:	SRR14016375_1.fastq
Paired file:	SRR14016375_2.fastq
trimmed:	SRR14016375-trimmed-pair1.fastq, SRR14016375-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG
-- paired 3' end adapter sequence (-y):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 05:55:04 2023 >> started

Thu May 11 05:56:10 2023 >> done (65.140s)
32031119 read pairs processed; of these:
     388 ( 0.00%) short read pairs filtered out after trimming by size control
 1413236 ( 4.41%) empty read pairs filtered out after trimming by size control
30617495 (95.59%) read pairs available; of these:
    3993 ( 0.01%) trimmed read pairs available after processing
30613502 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      86	  0.00%
 19	      99	  0.00%
 20	     156	  0.00%
 21	     294	  0.00%
 22	     816	  0.00%
 23	    1036	  0.00%
 24	    1117	  0.00%
 25	    1497	  0.00%
 26	    2036	  0.01%
 27	    2782	  0.01%
 28	    4252	  0.01%
 29	    5786	  0.02%
 30	    8259	  0.03%
 31	   11097	  0.04%
 32	   13018	  0.04%
 33	   13656	  0.04%
 34	   13926	  0.05%
 35	   15112	  0.05%
 36	   16499	  0.05%
 37	   17869	  0.06%
 38	   19138	  0.06%
 39	   20366	  0.07%
 40	   22142	  0.07%
 41	   23835	  0.08%
 42	   26006	  0.08%
 43	   27699	  0.09%
 44	   30222	  0.10%
 45	   31799	  0.10%
 46	   34189	  0.11%
 47	   35989	  0.12%
 48	   37828	  0.12%
 49	   40665	  0.13%
 50	   42824	  0.14%
 51	   46013	  0.15%
 52	   48408	  0.16%
 53	   51209	  0.17%
 54	   55096	  0.18%
 55	   58032	  0.19%
 56	   60503	  0.20%
 57	   63057	  0.21%
 58	   65235	  0.21%
 59	   68049	  0.22%
 60	   70579	  0.23%
 61	   73384	  0.24%
 62	   76964	  0.25%
 63	   80767	  0.26%
 64	   85211	  0.28%
 65	   88322	  0.29%
 66	   91518	  0.30%
 67	   93707	  0.31%
 68	   96540	  0.32%
 69	   99960	  0.33%
 70	  103346	  0.34%
 71	  107541	  0.35%
 72	  110835	  0.36%
 73	  114672	  0.37%
 74	  118389	  0.39%
 75	  121149	  0.40%
 76	  124933	  0.41%
 77	  126797	  0.41%
 78	  129514	  0.42%
 79	  131387	  0.43%
 80	  134000	  0.44%
 81	  136328	  0.45%
 82	  139209	  0.45%
 83	  142692	  0.47%
 84	  144512	  0.47%
 85	  148336	  0.48%
 86	  149134	  0.49%
 87	  151625	  0.50%
 88	  153611	  0.50%
 89	  154906	  0.51%
 90	  156288	  0.51%
 91	  157995	  0.52%
 92	  158994	  0.52%
 93	  161534	  0.53%
 94	  164569	  0.54%
 95	  164807	  0.54%
 96	  166686	  0.54%
 97	  168148	  0.55%
 98	  167476	  0.55%
 99	  167892	  0.55%
100	  170363	  0.56%
101	  170360	  0.56%
102	  170739	  0.56%
103	  172877	  0.56%
104	  172676	  0.56%
105	  171864	  0.56%
106	  172582	  0.56%
107	  171626	  0.56%
108	  172655	  0.56%
109	  173828	  0.57%
110	  172229	  0.56%
111	  172836	  0.56%
112	  172733	  0.56%
113	  173079	  0.57%
114	  173104	  0.57%
115	  173736	  0.57%
116	  173713	  0.57%
117	  173104	  0.57%
118	  172696	  0.56%
119	  169494	  0.55%
120	  171302	  0.56%
121	  170850	  0.56%
122	  169103	  0.55%
123	  170020	  0.56%
124	  168850	  0.55%
125	  168559	  0.55%
126	  168771	  0.55%
127	  169031	  0.55%
128	  167022	  0.55%
129	  165921	  0.54%
130	  166185	  0.54%
131	  164375	  0.54%
132	  163311	  0.53%
133	  164280	  0.54%
134	  162002	  0.53%
135	  162388	  0.53%
136	  161309	  0.53%
137	  161387	  0.53%
138	  159177	  0.52%
139	  157341	  0.51%
140	  156785	  0.51%
141	  155191	  0.51%
142	  155301	  0.51%
143	  154493	  0.50%
144	  151839	  0.50%
145	  152057	  0.50%
146	  151065	  0.49%
147	  150339	  0.49%
148	  153232	  0.50%
149	  161355	  0.53%
150	16146406	 52.74%


criterion=sequence-density
sequence-density=0.95
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=41
prefix-density=0.01
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=172.55
fanout-score-rank=1
prefix-density=0.36
prefix-fanout=4.3
sequence=GAGGAGAAGGAGGTGGGGAGGAGGTTTGGAGCACCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACATTGATGTCTTTGGGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGCATCAGCCCGTTT


criterion=sequence-density
sequence-density=0.91
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=42
prefix-density=0.01
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=178.02
fanout-score-rank=1
prefix-density=0.35
prefix-fanout=3.8
sequence=GAGGAGAAGGAGGTGGGGAGGAGGTTTGGAGCACCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGTCTTGTGAGTGGCCTCACAGGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACATTGATGTCTTTGGGGTAGAAGTTGTTCAAGAAGCACACGACTGAGGCACCTCCAGATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGCATCAGCCCGTTT
SRR14016375 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 11 05:59:35
                             Started mapping on |	May 11 05:59:36
                                    Finished on |	May 11 06:10:12
       Mapping speed, Million of reads per hour |	354.61

                          Number of input reads |	62648613
                      Average input read length |	256
                                    UNIQUE READS:
                   Uniquely mapped reads number |	51328917
                        Uniquely mapped reads % |	81.93%
                          Average mapped length |	250.13
                       Number of splices: Total |	44199380
            Number of splices: Annotated (sjdb) |	43409137
                       Number of splices: GT/AG |	43691846
                       Number of splices: GC/AG |	301860
                       Number of splices: AT/AC |	48365
               Number of splices: Non-canonical |	157309
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.75
                        Insertion rate per base |	0.01%
                       Insertion average length |	2.14
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2858550
             % of reads mapped to multiple loci |	4.56%
        Number of reads mapped to too many loci |	160167
             % of reads mapped to too many loci |	0.26%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.05%
                     % of reads unmapped: other |	0.20%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8461146	8461146	8461146
N_multimapping	2858550	2858550	2858550
N_noFeature	6204463	27883887	27741283
N_ambiguous	3035433	605864	607442
UnstrandedReadsAssigned:42089021 PositiveStrandReadsAssigned:22839166 NegativeStrandReadsAssigned:22980192
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=100 echo kmer=95
SRR14016375 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016375-trimmed-pair1.fastq
                             SRR14016375-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 62,648,613 reads, 47,347,076 reads pseudoaligned
[quant] estimated average fragment length: 152.691
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,146 rounds

 109283 SRR14016375.ke.tsv
  52636 SRR14016375.se.tsv
 161919 total
==> SRR14016375.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR14016375.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR14016375 completed mapping pipeline successfully
