Starting /dee2/code/volunteer_pipeline.sh SRR14016362
    current disk space = 308225176
    free memory = 263464128 
SRR14016362 SRAfilesize
66cacca7881fba6619cedc31f0a6d390  SRR14016362.sra
SRR14016362.sra file validated
SRR14016362 is paired end
SRR14016362 is conventional basespace
SRR14016362 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.489	37.0	37.0	37.0	37.0	37.0
2	35.372	37.0	37.0	37.0	37.0	37.0
3	35.543	37.0	37.0	37.0	37.0	37.0
4	35.797	37.0	37.0	37.0	37.0	37.0
5	35.9675	37.0	37.0	37.0	37.0	37.0
6	35.863	37.0	37.0	37.0	37.0	37.0
7	35.81	37.0	37.0	37.0	37.0	37.0
8	35.818	37.0	37.0	37.0	37.0	37.0
9	35.6705	37.0	37.0	37.0	37.0	37.0
10-14	35.731100000000005	37.0	37.0	37.0	37.0	37.0
15-19	35.6992	37.0	37.0	37.0	37.0	37.0
20-24	35.6349	37.0	37.0	37.0	37.0	37.0
25-29	35.379650000000005	37.0	37.0	37.0	37.0	37.0
30-34	35.37235	37.0	37.0	37.0	37.0	37.0
35-39	35.25095	37.0	37.0	37.0	34.6	37.0
40-44	35.18385	37.0	37.0	37.0	32.2	37.0
45-49	35.01515	37.0	37.0	37.0	25.0	37.0
50-54	34.94185	37.0	37.0	37.0	25.0	37.0
55-59	34.5195	37.0	37.0	37.0	25.0	37.0
60-64	34.32775	37.0	37.0	37.0	25.0	37.0
65-69	34.070499999999996	37.0	37.0	37.0	25.0	37.0
70-74	33.7563	37.0	37.0	37.0	25.0	37.0
75-79	33.696999999999996	37.0	37.0	37.0	25.0	37.0
80-84	33.634550000000004	37.0	37.0	37.0	22.2	37.0
85-89	33.4157	37.0	37.0	37.0	13.8	37.0
90-94	33.391949999999994	37.0	37.0	37.0	11.0	37.0
95-99	33.214150000000004	37.0	37.0	37.0	11.0	37.0
100-104	33.0518	37.0	37.0	37.0	11.0	37.0
105-109	32.773849999999996	37.0	37.0	37.0	11.0	37.0
110-114	32.532300000000006	37.0	37.0	37.0	11.0	37.0
115-119	32.3469	37.0	37.0	37.0	11.0	37.0
120-124	32.36895	37.0	37.0	37.0	11.0	37.0
125-129	32.155150000000006	37.0	37.0	37.0	11.0	37.0
130-134	31.95725	37.0	37.0	37.0	11.0	37.0
135-139	31.996699999999997	37.0	34.6	37.0	11.0	37.0
140-144	31.9789	37.0	37.0	37.0	11.0	37.0
145-149	31.849	37.0	32.2	37.0	11.0	37.0
150	31.82375	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	0.0
17	0.0
18	2.0
19	2.0
20	8.0
21	13.0
22	32.0
23	58.0
24	86.0
25	82.0
26	95.0
27	107.0
28	102.0
29	82.0
30	129.0
31	121.0
32	158.0
33	218.0
34	373.0
35	523.0
36	1690.0
37	117.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.139069534767387	21.935967983991997	20.4352176088044	29.48974487243622
2	14.88988988988989	28.003003003003002	35.38538538538539	21.72172172172172
3	16.0	26.450000000000003	37.15	20.4
4	18.075	37.0	25.85	19.075
5	18.775	38.525	24.55	18.15
6	19.35	32.175	30.475	18.0
7	18.575	27.025	31.25	23.150000000000002
8	20.549999999999997	32.675	26.75	20.025000000000002
9	18.41841841841842	25.525525525525527	35.33533533533533	20.72072072072072
10-14	19.375	31.1	28.96	20.565
15-19	20.875	29.42	28.67	21.035
20-24	21.295	30.285	30.645	17.775
25-29	19.220961048052402	30.221511075553774	29.951497574878744	20.606030301515077
30-34	20.022007702695944	29.760416145650975	31.245936077627167	18.97164007402591
35-39	17.684421105276318	30.417604401100274	33.083270817704424	18.814703675918977
40-44	19.42791418712807	29.61444216632495	33.73506025903886	17.222583387508124
45-49	18.767815172275842	28.824323648547285	34.42516377456619	17.982697404610693
50-54	18.699674918729684	29.83245811452863	33.683420855213804	17.78444611152788
55-59	19.33386677335467	31.176235247049412	31.926385277055413	17.563512702540507
60-64	19.499874968742187	31.15778944736184	31.472868217054263	17.86946736684171
65-69	19.410823246974093	31.56947084125238	30.219065719715914	18.800640192057617
70-74	20.08602580774232	32.49974992497749	28.02840852255677	19.385815744723416
75-79	20.336100830249073	31.73952185655697	28.133440032009606	19.790937281184355
80-84	19.55575566561609	32.497873830606835	27.880334183801093	20.066036319975986
85-89	19.91296518607443	31.57262905162065	28.061224489795915	20.453181272509003
90-94	19.738882497123704	31.689260167075183	28.452803761692763	20.11905357410835
95-99	19.80391176029213	31.999399729878448	27.382322044920215	20.81436646490921
100-104	19.18267306922769	32.578031212484994	27.425970388155264	20.813325330132052
105-109	19.378720424190888	32.61467660447202	27.452353559101596	20.554249412235507
110-114	20.413165266106443	32.6530612244898	27.045818327330934	19.88795518207283
115-119	21.091327398219466	31.834550365109532	26.718015404621386	20.356106832049615
120-124	21.40249087180513	32.6414244985745	26.4792677437103	19.47681688591007
125-129	22.8202691211045	31.519183632634686	25.926667000150065	19.73388024611075
130-134	23.868353923873357	31.320962336817885	25.453908868103838	19.35677487120492
135-139	25.822746824047215	30.414124237271185	24.762428728618584	19.00070021006302
140-144	27.173151945583673	30.244073221966588	24.222266680004	18.360508152445735
145-149	28.517110266159694	29.122473484090456	23.76425855513308	18.596157694616767
150	31.207801950487625	28.732183045761438	21.655413853463365	18.404601150287572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	0.5
5	0.5
6	1.0
7	1.5
8	2.0
9	2.5
10	3.0
11	2.5
12	4.0
13	5.5
14	7.5
15	11.5
16	13.5
17	19.0
18	23.5
19	24.5
20	25.0
21	26.5
22	26.5
23	29.0
24	37.0
25	55.5
26	64.5
27	56.0
28	64.5
29	79.0
30	85.5
31	97.5
32	120.0
33	140.0
34	151.5
35	154.5
36	161.0
37	163.5
38	150.0
39	148.5
40	157.5
41	162.5
42	159.5
43	143.5
44	146.5
45	146.5
46	138.0
47	140.5
48	129.5
49	125.5
50	109.0
51	88.0
52	76.0
53	66.0
54	55.5
55	37.5
56	26.5
57	22.0
58	20.5
59	17.5
60	18.0
61	13.5
62	5.5
63	8.0
64	10.0
65	6.5
66	4.0
67	1.0
68	0.5
69	1.0
70	0.5
71	0.0
72	0.5
73	0.5
74	0.5
75	0.5
76	0.0
77	0.0
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.1
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.1
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.034999999999999996
35-39	0.025
40-44	0.015
45-49	0.015
50-54	0.025
55-59	0.02
60-64	0.025
65-69	0.03
70-74	0.03
75-79	0.03
80-84	0.055
85-89	0.04
90-94	0.045
95-99	0.045
100-104	0.04
105-109	0.045
110-114	0.04
115-119	0.03
120-124	0.034999999999999996
125-129	0.045
130-134	0.034999999999999996
135-139	0.03
140-144	0.03
145-149	0.06
150	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.57241181531403	80.7
2	6.395182104961285	11.15
3	0.5735589331803843	1.5
4	0.2581015199311729	0.8999999999999999
5	0.05735589331803843	0.25
6	0.028677946659019213	0.15
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.05735589331803843	0.7000000000000001
>50	0.028677946659019213	1.8499999999999999
>100	0.028677946659019213	2.8000000000000003
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	112	2.8000000000000003	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	74	1.8499999999999999	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.42500000000000004	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
GATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAA	5	0.125	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0125	0.0	0.0	0.0	0.0
32-33	0.037500000000000006	0.0	0.0	0.0	0.0
34-35	0.15	0.0	0.0	0.0	0.0
36-37	0.2625	0.0	0.0	0.0	0.0
38-39	0.4375	0.0	0.0	0.0	0.0
40-41	0.5875	0.0	0.0	0.0	0.0
42-43	0.85	0.0	0.0	0.0	0.0
44-45	1.3	0.0	0.0	0.0	0.0
46-47	1.6124999999999998	0.0	0.0	0.0	0.0
48-49	1.825	0.0	0.0	0.0	0.0
50-51	2.2125000000000004	0.0	0.0	0.0	0.0
52-53	2.5625	0.0	0.0	0.0	0.0
54-55	3.0375	0.0	0.0	0.0	0.0
56-57	3.6	0.0	0.0	0.0	0.0
58-59	4.137499999999999	0.0	0.0	0.0	0.0
60-61	4.737500000000001	0.0	0.0	0.0	0.0
62-63	5.275	0.0	0.0	0.0	0.0
64-65	5.9125	0.0	0.0	0.0	0.0
66-67	6.55	0.0	0.0	0.0	0.0
68-69	7.2	0.0	0.0	0.0	0.0
70-71	7.85	0.0	0.0	0.0	0.0
72-73	8.575	0.0	0.0	0.0	0.0
74-75	9.2625	0.0	0.0	0.0	0.0
76-77	9.912500000000001	0.0	0.0	0.0	0.0
78-79	10.4375	0.0	0.0	0.0	0.0
80-81	10.925	0.0	0.0	0.0	0.0
82-83	11.5875	0.0	0.0	0.0	0.0
84-85	12.3125	0.0	0.0	0.0	0.0
86-87	12.925	0.0	0.0	0.0	0.0
88-89	13.537500000000001	0.0	0.0	0.0	0.0
90-91	14.287500000000001	0.0	0.0	0.0	0.0
92-93	14.725	0.0	0.0	0.0	0.0
94-95	15.2625	0.0	0.0	0.0	0.0
96-97	16.0375	0.0	0.0	0.0	0.0
98-99	16.65	0.0	0.0	0.0	0.0
100-101	17.3	0.0	0.0	0.0	0.0
102-103	17.9125	0.0	0.0	0.0	0.0
104-105	18.6375	0.0	0.0	0.0	0.0
106-107	19.15	0.0	0.0	0.0	0.0
108-109	19.95	0.0	0.0	0.0	0.0
110-111	20.725	0.0	0.0	0.0	0.0
112-113	21.375	0.0	0.0	0.0	0.0
114-115	21.862499999999997	0.0	0.0	0.0	0.0
116-117	22.35	0.0	0.0	0.0	0.0
118-119	22.7	0.0	0.0	0.0	0.0
120-121	23.3875	0.0	0.0	0.0	0.0
122-123	24.15	0.0	0.0	0.0	0.0
124-125	24.825000000000003	0.0	0.0	0.0	0.0
126-127	25.35	0.0	0.0	0.0	0.0
128-129	25.9625	0.0	0.0	0.0	0.0
130-131	26.549999999999997	0.0	0.0	0.0	0.0
132-133	26.975	0.0	0.0	0.0	0.0
134-135	27.5625	0.0	0.0	0.0	0.0
136-137	28.1875	0.0	0.0	0.0	0.0
138	28.675	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	15	1.11538764E-4	145.79747	1
CTAATCA	15	1.11538764E-4	145.79747	2
CTCTTCA	10	0.0069772652	143.975	6
TTGCAAG	20	3.6904166E-4	107.981255	9
AATCATT	20	3.6904166E-4	107.981255	4
ATTGCAA	20	3.6904166E-4	107.981255	8
ATCATTG	20	3.6904166E-4	107.981255	5
TCATTGC	20	3.6904166E-4	107.981255	6
CATTGCA	25	8.9624035E-4	86.385	7
TAATCAT	25	8.9624035E-4	86.385	3
GGGGGGG	1335	0.0	6.470786	140-144
>>END_MODULE
SRR14016362 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016362_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.3435	37.0	37.0	37.0	25.0	37.0
2	34.4015	37.0	37.0	37.0	25.0	37.0
3	34.6945	37.0	37.0	37.0	25.0	37.0
4	34.6925	37.0	37.0	37.0	25.0	37.0
5	34.7925	37.0	37.0	37.0	25.0	37.0
6	34.9435	37.0	37.0	37.0	25.0	37.0
7	34.8815	37.0	37.0	37.0	25.0	37.0
8	34.9835	37.0	37.0	37.0	25.0	37.0
9	34.99	37.0	37.0	37.0	25.0	37.0
10-14	34.93150000000001	37.0	37.0	37.0	25.0	37.0
15-19	35.0364	37.0	37.0	37.0	25.0	37.0
20-24	34.9021	37.0	37.0	37.0	25.0	37.0
25-29	34.8029	37.0	37.0	37.0	25.0	37.0
30-34	34.8738	37.0	37.0	37.0	25.0	37.0
35-39	34.7156	37.0	37.0	37.0	25.0	37.0
40-44	34.6389	37.0	37.0	37.0	25.0	37.0
45-49	34.4203	37.0	37.0	37.0	25.0	37.0
50-54	34.172	37.0	37.0	37.0	25.0	37.0
55-59	33.9172	37.0	37.0	37.0	25.0	37.0
60-64	33.814299999999996	37.0	37.0	37.0	25.0	37.0
65-69	33.3896	37.0	37.0	37.0	13.8	37.0
70-74	33.1279	37.0	37.0	37.0	11.0	37.0
75-79	33.03249999999999	37.0	37.0	37.0	11.0	37.0
80-84	33.0769	37.0	37.0	37.0	11.0	37.0
85-89	32.8356	37.0	37.0	37.0	11.0	37.0
90-94	32.891000000000005	37.0	37.0	37.0	11.0	37.0
95-99	32.6631	37.0	37.0	37.0	11.0	37.0
100-104	32.5861	37.0	37.0	37.0	11.0	37.0
105-109	32.4362	37.0	37.0	37.0	11.0	37.0
110-114	32.2646	37.0	37.0	37.0	11.0	37.0
115-119	32.0762	37.0	37.0	37.0	11.0	37.0
120-124	31.8902	37.0	32.2	37.0	11.0	37.0
125-129	31.6589	37.0	25.0	37.0	11.0	37.0
130-134	31.7181	37.0	25.0	37.0	11.0	37.0
135-139	31.4555	37.0	25.0	37.0	11.0	37.0
140-144	31.5545	37.0	25.0	37.0	11.0	37.0
145-149	31.5671	37.0	25.0	37.0	11.0	37.0
150	31.208	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	2.0
18	1.0
19	5.0
20	11.0
21	17.0
22	39.0
23	57.0
24	107.0
25	114.0
26	126.0
27	111.0
28	88.0
29	116.0
30	105.0
31	134.0
32	181.0
33	292.0
34	435.0
35	798.0
36	1216.0
37	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.88844422211106	23.011505752876438	19.559779889944974	30.540270135067534
2	15.174999999999999	27.875	35.55	21.4
3	18.0	26.625	35.699999999999996	19.675
4	17.75	35.975	25.025	21.25
5	19.775000000000002	39.425	24.15	16.650000000000002
6	21.05	32.4	28.4	18.15
7	18.125	27.375	31.8	22.7
8	20.75	32.2	28.725	18.325
9	19.625	24.525	36.375	19.475
10-14	20.23	30.830000000000002	28.49	20.45
15-19	21.23	30.12	28.134999999999998	20.515
20-24	21.57	29.615000000000002	30.659999999999997	18.154999999999998
25-29	20.21	29.965000000000003	29.45	20.375
30-34	20.74	30.659999999999997	29.81	18.790000000000003
35-39	17.705000000000002	30.995	32.72	18.58
40-44	20.285	29.475	32.635	17.605
45-49	18.73	30.490000000000002	32.79	17.990000000000002
50-54	19.225	30.25	32.35	18.175
55-59	19.915	30.305	31.695	18.085
60-64	19.445	31.295	31.290000000000003	17.97
65-69	20.515	31.480000000000004	30.049999999999997	17.955
70-74	21.515	32.39	28.62	17.474999999999998
75-79	22.314999999999998	31.915	28.395	17.375
80-84	22.470000000000002	31.730000000000004	28.68	17.119999999999997
85-89	23.145	32.22	27.639999999999997	16.994999999999997
90-94	23.62	32.105	26.935	17.34
95-99	22.99	33.055	26.735	17.22
100-104	22.71	33.18	27.26	16.85
105-109	24.075	32.34	27.215	16.37
110-114	24.355	32.93	26.46	16.255
115-119	25.455	32.855000000000004	25.41	16.28
120-124	26.805	31.900000000000002	25.52	15.775
125-129	27.96	31.71	25.2	15.129999999999999
130-134	29.45	32.035000000000004	23.575	14.940000000000001
135-139	30.995	30.635	23.794999999999998	14.575
140-144	32.04	30.130000000000003	23.43	14.399999999999999
145-149	33.625	29.604999999999997	22.814999999999998	13.955
150	36.575	28.075	21.8	13.55
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.5
3	1.5
4	1.5
5	1.0
6	0.5
7	1.0
8	1.5
9	1.5
10	2.0
11	3.0
12	6.5
13	7.5
14	10.5
15	12.5
16	12.5
17	15.5
18	16.0
19	16.5
20	20.5
21	26.5
22	36.5
23	40.0
24	41.5
25	52.0
26	60.5
27	59.5
28	58.0
29	76.5
30	86.0
31	93.0
32	120.0
33	122.5
34	137.5
35	161.0
36	154.5
37	164.0
38	165.5
39	161.5
40	167.5
41	159.5
42	163.0
43	154.5
44	133.0
45	146.0
46	145.0
47	129.0
48	113.5
49	100.5
50	100.5
51	92.0
52	77.0
53	63.5
54	55.0
55	49.0
56	40.0
57	30.0
58	23.0
59	18.5
60	17.0
61	13.0
62	10.5
63	10.5
64	9.5
65	6.5
66	4.5
67	2.5
68	1.0
69	1.0
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	1.0
77	1.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.87015881861242	84.22500000000001
2	5.433268319866258	9.75
3	0.4736695458344943	1.275
4	0.02786291446085261	0.1
5	0.08358874338255781	0.375
6	0.02786291446085261	0.15
7	0.0	0.0
8	0.02786291446085261	0.2
9	0.0	0.0
>10	0.0	0.0
>50	0.05572582892170522	3.925
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	89	2.225	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	68	1.7000000000000002	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.15	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
AGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCT	5	0.125	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0125	0.0	0.0	0.0	0.0
32-33	0.05	0.0	0.0	0.0	0.0
34-35	0.15	0.0	0.0	0.0	0.0
36-37	0.2625	0.0	0.0	0.0	0.0
38-39	0.4375	0.0	0.0	0.0	0.0
40-41	0.5625	0.0	0.0	0.0	0.0
42-43	0.825	0.0	0.0	0.0	0.0
44-45	1.3	0.0	0.0	0.0	0.0
46-47	1.6375000000000002	0.0	0.0	0.0	0.0
48-49	1.8624999999999998	0.0	0.0	0.0	0.0
50-51	2.2375	0.0	0.0	0.0	0.0
52-53	2.6625	0.0	0.0	0.0	0.0
54-55	3.1375	0.0	0.0	0.0	0.0
56-57	3.7	0.0	0.0	0.0	0.0
58-59	4.2125	0.0	0.0	0.0	0.0
60-61	4.824999999999999	0.0	0.0	0.0	0.0
62-63	5.3375	0.0	0.0	0.0	0.0
64-65	5.9375	0.0	0.0	0.0	0.0
66-67	6.475	0.0	0.0	0.0	0.0
68-69	7.125	0.0	0.0	0.0	0.0
70-71	7.7625	0.0	0.0	0.0	0.0
72-73	8.5	0.0	0.0	0.0	0.0
74-75	9.1625	0.0	0.0	0.0	0.0
76-77	9.8125	0.0	0.0	0.0	0.0
78-79	10.3125	0.0	0.0	0.0	0.0
80-81	10.8625	0.0	0.0	0.0	0.0
82-83	11.5	0.0	0.0	0.0	0.0
84-85	12.1875	0.0	0.0	0.0	0.0
86-87	12.774999999999999	0.0	0.0	0.0	0.0
88-89	13.3875	0.0	0.0	0.0	0.0
90-91	14.2	0.0	0.0	0.0	0.0
92-93	14.7125	0.0	0.0	0.0	0.0
94-95	15.274999999999999	0.0	0.0	0.0	0.0
96-97	15.9375	0.0	0.0	0.0	0.0
98-99	16.475	0.0	0.0	0.0	0.0
100-101	17.0375	0.0	0.0	0.0	0.0
102-103	17.6125	0.0	0.0	0.0	0.0
104-105	18.4125	0.0	0.0	0.0	0.0
106-107	18.9625	0.0	0.0	0.0	0.0
108-109	19.75	0.0	0.0	0.0	0.0
110-111	20.525	0.0	0.0	0.0	0.0
112-113	21.1875	0.0	0.0	0.0	0.0
114-115	21.6125	0.0	0.0	0.0	0.0
116-117	22.075	0.0	0.0	0.0	0.0
118-119	22.4625	0.0	0.0	0.0	0.0
120-121	23.075	0.0	0.0	0.0	0.0
122-123	23.825	0.0	0.0	0.0	0.0
124-125	24.450000000000003	0.0	0.0	0.0	0.0
126-127	24.987499999999997	0.0	0.0	0.0	0.0
128-129	25.5375	0.0	0.0	0.0	0.0
130-131	26.137500000000003	0.0	0.0	0.0	0.0
132-133	26.625	0.0	0.0	0.0	0.0
134-135	27.2125	0.0	0.0	0.0	0.0
136-137	27.85	0.0	0.0	0.0	0.0
138	28.35	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCAAG	20	1.9452218E-6	144.0	9
CCTAATT	10	0.006973645	144.0	2
ATTGCAA	20	1.9452218E-6	144.0	8
CATTGCA	25	5.904236E-6	115.2	7
AATCATT	25	5.904236E-6	115.2	4
GCTAATC	25	5.904236E-6	115.2	1
CTAATCA	25	5.904236E-6	115.2	2
TAATCAT	25	5.904236E-6	115.2	3
ATCATTG	25	5.904236E-6	115.2	5
TCATTGC	25	5.904236E-6	115.2	6
TGGTATC	45	1.0653384E-6	80.0	7
AGTGGTA	45	1.0653384E-6	80.0	5
GTATCAA	45	1.0653384E-6	80.0	9
GCAGTGG	45	1.0653384E-6	80.0	3
CAGTGGT	45	1.0653384E-6	80.0	4
AAGCAGT	45	1.0653384E-6	80.0	1
AGCAGTG	50	1.9936197E-6	72.0	2
GTGGTAT	50	1.9936197E-6	72.0	6
GGTATCA	50	1.9936197E-6	72.0	8
GCAAGCA	20	0.006139246	28.8	10-14
>>END_MODULE
2023-05-11T13:33:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:33:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:33:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:33:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:33:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:33:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:33:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:33:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:34:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
Written 485145 spots for SRR14016362.sra
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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2023-05-11T13:34:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T13:34:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 485145 spots for SRR14016362.sra
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2023-05-11T13:34:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
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SRR ids: ['SRR14016362.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qm_rbzr9
SRR14016362.sra spots: 31049306
blocks: [[1, 485145], [485146, 970290], [970291, 1455435], [1455436, 1940580], [1940581, 2425725], [2425726, 2910870], [2910871, 3396015], [3396016, 3881160], [3881161, 4366305], [4366306, 4851450], [4851451, 5336595], [5336596, 5821740], [5821741, 6306885], [6306886, 6792030], [6792031, 7277175], [7277176, 7762320], [7762321, 8247465], [8247466, 8732610], [8732611, 9217755], [9217756, 9702900], [9702901, 10188045], [10188046, 10673190], [10673191, 11158335], [11158336, 11643480], [11643481, 12128625], [12128626, 12613770], [12613771, 13098915], [13098916, 13584060], [13584061, 14069205], [14069206, 14554350], [14554351, 15039495], [15039496, 15524640], [15524641, 16009785], [16009786, 16494930], [16494931, 16980075], [16980076, 17465220], [17465221, 17950365], [17950366, 18435510], [18435511, 18920655], [18920656, 19405800], [19405801, 19890945], [19890946, 20376090], [20376091, 20861235], [20861236, 21346380], [21346381, 21831525], [21831526, 22316670], [22316671, 22801815], [22801816, 23286960], [23286961, 23772105], [23772106, 24257250], [24257251, 24742395], [24742396, 25227540], [25227541, 25712685], [25712686, 26197830], [26197831, 26682975], [26682976, 27168120], [27168121, 27653265], [27653266, 28138410], [28138411, 28623555], [28623556, 29108700], [29108701, 29593845], [29593846, 30078990], [30078991, 30564135], [30564136, 31049306]]
SRR14016362 file size 10469576
SRR14016362 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016362 SRR14016362_1.fastq SRR14016362_2.fastq
Input file:	SRR14016362_1.fastq
Paired file:	SRR14016362_2.fastq
trimmed:	SRR14016362-trimmed-pair1.fastq, SRR14016362-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 13:35:11 2023 >> started

Thu May 11 13:36:14 2023 >> done (62.141s)
31049306 read pairs processed; of these:
    6586 ( 0.02%) short read pairs filtered out after trimming by size control
    4704 ( 0.02%) empty read pairs filtered out after trimming by size control
31038016 (99.96%) read pairs available; of these:
12790704 (41.21%) trimmed read pairs available after processing
18247312 (58.79%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     430	  0.00%
 19	     354	  0.00%
 20	     836	  0.00%
 21	    1018	  0.00%
 22	    2595	  0.01%
 23	    1912	  0.01%
 24	    1944	  0.01%
 25	    2592	  0.01%
 26	    3869	  0.01%
 27	    5843	  0.02%
 28	    9447	  0.03%
 29	   13269	  0.04%
 30	   19315	  0.06%
 31	   27552	  0.09%
 32	   38828	  0.13%
 33	   38304	  0.12%
 34	   47878	  0.15%
 35	   45884	  0.15%
 36	   44470	  0.14%
 37	   50114	  0.16%
 38	   52581	  0.17%
 39	   59711	  0.19%
 40	   61176	  0.20%
 41	   67772	  0.22%
 42	   88328	  0.28%
 43	   74848	  0.24%
 44	   77461	  0.25%
 45	   78733	  0.25%
 46	   80228	  0.26%
 47	   83067	  0.27%
 48	   85378	  0.28%
 49	   89240	  0.29%
 50	   92190	  0.30%
 51	   95461	  0.31%
 52	   99626	  0.32%
 53	  104372	  0.34%
 54	  104728	  0.34%
 55	  105659	  0.34%
 56	  106745	  0.34%
 57	  106973	  0.34%
 58	  107278	  0.35%
 59	  107359	  0.35%
 60	  108722	  0.35%
 61	  109653	  0.35%
 62	  110694	  0.36%
 63	  112508	  0.36%
 64	  113734	  0.37%
 65	  114355	  0.37%
 66	  115184	  0.37%
 67	  115299	  0.37%
 68	  115933	  0.37%
 69	  116349	  0.37%
 70	  116307	  0.37%
 71	  116997	  0.38%
 72	  118894	  0.38%
 73	  119728	  0.39%
 74	  120586	  0.39%
 75	  120437	  0.39%
 76	  121149	  0.39%
 77	  120757	  0.39%
 78	  120334	  0.39%
 79	  119856	  0.39%
 80	  120605	  0.39%
 81	  121018	  0.39%
 82	  121300	  0.39%
 83	  121735	  0.39%
 84	  122279	  0.39%
 85	  123069	  0.40%
 86	  121775	  0.39%
 87	  121886	  0.39%
 88	  122205	  0.39%
 89	  121946	  0.39%
 90	  122142	  0.39%
 91	  121991	  0.39%
 92	  122300	  0.39%
 93	  122989	  0.40%
 94	  122328	  0.39%
 95	  123232	  0.40%
 96	  122733	  0.40%
 97	  122933	  0.40%
 98	  121855	  0.39%
 99	  121246	  0.39%
100	  122280	  0.39%
101	  121086	  0.39%
102	  121892	  0.39%
103	  122044	  0.39%
104	  121895	  0.39%
105	  121354	  0.39%
106	  120667	  0.39%
107	  120529	  0.39%
108	  120192	  0.39%
109	  119718	  0.39%
110	  118839	  0.38%
111	  118254	  0.38%
112	  118877	  0.38%
113	  119009	  0.38%
114	  118351	  0.38%
115	  118695	  0.38%
116	  118411	  0.38%
117	  117892	  0.38%
118	  117746	  0.38%
119	  116495	  0.38%
120	  115724	  0.37%
121	  115883	  0.37%
122	  114971	  0.37%
123	  115499	  0.37%
124	  115119	  0.37%
125	  115654	  0.37%
126	  114966	  0.37%
127	  114537	  0.37%
128	  113701	  0.37%
129	  113077	  0.36%
130	  112629	  0.36%
131	  112144	  0.36%
132	  111979	  0.36%
133	  111778	  0.36%
134	  111885	  0.36%
135	  110975	  0.36%
136	  111020	  0.36%
137	  110622	  0.36%
138	  110095	  0.35%
139	  109739	  0.35%
140	  109143	  0.35%
141	  108696	  0.35%
142	  108475	  0.35%
143	  108476	  0.35%
144	  106585	  0.34%
145	  106399	  0.34%
146	  107042	  0.34%
147	  107212	  0.35%
148	  106517	  0.34%
149	  111525	  0.36%
150	18247312	 58.79%
31038016 reads passed initial QC


criterion=sequence-density
sequence-density=4.71
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=41
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=1.15
sequence-density-rank=2
fanout-score=31.70
fanout-score-rank=1
prefix-density=1.16
prefix-fanout=31.4
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCAGATCGGAA


criterion=sequence-density
sequence-density=4.86
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=40
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG


criterion=fanout-score
sequence-density=0.13
sequence-density-rank=30
fanout-score=32.27
fanout-score-rank=1
prefix-density=3.99
prefix-fanout=1.0
sequence=AGTACATGGGTATGCCACAACT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG -y GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG -o SRR14016362 SRR14016362_1.fastq SRR14016362_2.fastq
Input file:	SRR14016362_1.fastq
Paired file:	SRR14016362_2.fastq
trimmed:	SRR14016362-trimmed-pair1.fastq, SRR14016362-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG
-- paired 3' end adapter sequence (-y):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 13:39:38 2023 >> started

Thu May 11 13:40:15 2023 >> done (36.465s)
18622810 read pairs processed; of these:
     304 ( 0.00%) short read pairs filtered out after trimming by size control
 1179645 ( 6.33%) empty read pairs filtered out after trimming by size control
17442861 (93.66%) read pairs available; of these:
    1133 ( 0.01%) trimmed read pairs available after processing
17441728 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     157	  0.00%
 19	     162	  0.00%
 20	     363	  0.00%
 21	     366	  0.00%
 22	    1222	  0.01%
 23	    1020	  0.01%
 24	    1021	  0.01%
 25	    1440	  0.01%
 26	    2213	  0.01%
 27	    3396	  0.02%
 28	    5149	  0.03%
 29	    7603	  0.04%
 30	   10862	  0.06%
 31	   15496	  0.09%
 32	   20448	  0.12%
 33	   20799	  0.12%
 34	   21729	  0.12%
 35	   23803	  0.14%
 36	   25129	  0.14%
 37	   27290	  0.16%
 38	   30230	  0.17%
 39	   31831	  0.18%
 40	   34535	  0.20%
 41	   36939	  0.21%
 42	   39471	  0.23%
 43	   41453	  0.24%
 44	   43909	  0.25%
 45	   45710	  0.26%
 46	   47632	  0.27%
 47	   48826	  0.28%
 48	   50370	  0.29%
 49	   52340	  0.30%
 50	   53245	  0.31%
 51	   54673	  0.31%
 52	   56598	  0.32%
 53	   57894	  0.33%
 54	   59290	  0.34%
 55	   60002	  0.34%
 56	   60207	  0.35%
 57	   60641	  0.35%
 58	   61144	  0.35%
 59	   61193	  0.35%
 60	   62227	  0.36%
 61	   62371	  0.36%
 62	   63264	  0.36%
 63	   64272	  0.37%
 64	   65079	  0.37%
 65	   65116	  0.37%
 66	   66042	  0.38%
 67	   65746	  0.38%
 68	   66270	  0.38%
 69	   66541	  0.38%
 70	   66471	  0.38%
 71	   66919	  0.38%
 72	   67805	  0.39%
 73	   68057	  0.39%
 74	   68605	  0.39%
 75	   68471	  0.39%
 76	   68560	  0.39%
 77	   68597	  0.39%
 78	   68593	  0.39%
 79	   68272	  0.39%
 80	   68327	  0.39%
 81	   68841	  0.39%
 82	   69178	  0.40%
 83	   69038	  0.40%
 84	   69080	  0.40%
 85	   69872	  0.40%
 86	   68798	  0.39%
 87	   69202	  0.40%
 88	   68986	  0.40%
 89	   68994	  0.40%
 90	   69030	  0.40%
 91	   69248	  0.40%
 92	   69230	  0.40%
 93	   69792	  0.40%
 94	   69218	  0.40%
 95	   69840	  0.40%
 96	   69369	  0.40%
 97	   69748	  0.40%
 98	   68793	  0.39%
 99	   68378	  0.39%
100	   68309	  0.39%
101	   68660	  0.39%
102	   68595	  0.39%
103	   68286	  0.39%
104	   69036	  0.40%
105	   67855	  0.39%
106	   67913	  0.39%
107	   67981	  0.39%
108	   67842	  0.39%
109	   67716	  0.39%
110	   66806	  0.38%
111	   66790	  0.38%
112	   67557	  0.39%
113	   66868	  0.38%
114	   66262	  0.38%
115	   66634	  0.38%
116	   66787	  0.38%
117	   66153	  0.38%
118	   65814	  0.38%
119	   65501	  0.38%
120	   65334	  0.37%
121	   64845	  0.37%
122	   64347	  0.37%
123	   64908	  0.37%
124	   64608	  0.37%
125	   64870	  0.37%
126	   64396	  0.37%
127	   64023	  0.37%
128	   63918	  0.37%
129	   63612	  0.36%
130	   62842	  0.36%
131	   62868	  0.36%
132	   63054	  0.36%
133	   62354	  0.36%
134	   62539	  0.36%
135	   62134	  0.36%
136	   62248	  0.36%
137	   61781	  0.35%
138	   61684	  0.35%
139	   61538	  0.35%
140	   61464	  0.35%
141	   60559	  0.35%
142	   60560	  0.35%
143	   60774	  0.35%
144	   59522	  0.34%
145	   59566	  0.34%
146	   60074	  0.34%
147	   60142	  0.34%
148	   59950	  0.34%
149	   62378	  0.36%
150	10234563	 58.67%


criterion=sequence-density
sequence-density=0.34
sequence-density-rank=1
fanout-score=24.08
fanout-score-rank=2
prefix-density=0.31
prefix-fanout=24.1
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCAGA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=33
fanout-score=29.58
fanout-score-rank=1
prefix-density=0.20
prefix-fanout=2.9
sequence=GCAGCAGCGCCTGTGGGAAAAACTAGAGGCTAGAGCCATGGATGACCAACGCGACCTCATCTCTAACCATGAACAGTTGCCCATACTGGGCAACCGCCCTAGAGAGCCAGAAAGGTGCAGCCGTGGAGCTCTGTACACCGGTGTCTCTGTCCTGGTGGCTCTGCTCTTGGCTGGGCAGGCCACCACTGCTTACTTCCTGTACCAGCAACAGGGCCGCCTAGACAAGCTGACCATCACCTCCCAGAACCTGCAACTGGAGAGCCTTCGCATGAAGCTTCCGAAATCTGCCAAACCTGTGAGCCAGATGCGGATGGCTACTCCCTTGCTGATGCGTCCAATGTCCATGGATAACATGCTCCTTGGGCCTGTGAAGAACGTTACCAAGTACGGCAACATGACCCAGGACCATGTGATGCATCTGCTCACGAGGTCTGGACCCCTGGAGTACCCGCAGCTGAAGGGGACCTTCCCAGAGAATCTGAAGCATCTTAAGAACTCCATGGATGGCGTG


criterion=sequence-density
sequence-density=0.34
sequence-density-rank=1
fanout-score=25.23
fanout-score-rank=3
prefix-density=0.32
prefix-fanout=25.2
sequence=CCCCATGTACTCTGCGTTGATACCACTGCTTGCAATGATTAGCA


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=12
fanout-score=45.25
fanout-score-rank=1
prefix-density=5.26
prefix-fanout=1.0
sequence=ACGCAGAGTACT
SRR14016362 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 11 13:42:40
                             Started mapping on |	May 11 13:42:41
                                    Finished on |	May 11 13:45:46
       Mapping speed, Million of reads per hour |	581.02

                          Number of input reads |	29858067
                      Average input read length |	253
                                    UNIQUE READS:
                   Uniquely mapped reads number |	24087269
                        Uniquely mapped reads % |	80.67%
                          Average mapped length |	246.79
                       Number of splices: Total |	16089535
            Number of splices: Annotated (sjdb) |	15783402
                       Number of splices: GT/AG |	15871128
                       Number of splices: GC/AG |	118380
                       Number of splices: AT/AC |	16822
               Number of splices: Non-canonical |	83205
                      Mismatch rate per base, % |	0.35%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.78
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.36
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1161593
             % of reads mapped to multiple loci |	3.89%
        Number of reads mapped to too many loci |	102321
             % of reads mapped to too many loci |	0.34%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.68%
                     % of reads unmapped: other |	0.41%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4609205	4609205	4609205
N_multimapping	1161593	1161593	1161593
N_noFeature	7894257	15680333	15644767
N_ambiguous	1084783	242993	244386
UnstrandedReadsAssigned:15108229 PositiveStrandReadsAssigned:8163943 NegativeStrandReadsAssigned:8198116
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=92 echo kmer=87
SRR14016362 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016362-trimmed-pair1.fastq
                             SRR14016362-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 29,858,067 reads, 17,668,692 reads pseudoaligned
[quant] estimated average fragment length: 162.248
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,031 rounds

 109283 SRR14016362.ke.tsv
  52636 SRR14016362.se.tsv
 161919 total
==> SRR14016362.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	6.5	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	1.5	0	0
ENSMUST00000178537.1	12	2.5	0	0
ENSMUST00000178862.1	14	4.5	0	0
ENSMUST00000179520.1	11	1.5	0	0
ENSMUST00000179883.1	16	6.5	0	0
ENSMUST00000195858.1	10	1	0	0
ENSMUST00000179932.1	12	2.5	0	0

==> SRR14016362.se.tsv <==
ENSMUSG00000102693	1
ENSMUSG00000064842	0
ENSMUSG00000051951	3
ENSMUSG00000102851	1
ENSMUSG00000103377	8
ENSMUSG00000104017	6
ENSMUSG00000103025	8
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	1
SRR14016362 completed mapping pipeline successfully
