Starting /dee2/code/volunteer_pipeline.sh SRR14016358
    current disk space = 316821644
    free memory = 263484280 
SRR14016358 SRAfilesize
458eb8d49217cdc950db55f6b5f72a14  SRR14016358.sra
SRR14016358.sra file validated
SRR14016358 is paired end
SRR14016358 is conventional basespace
SRR14016358 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016358_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.2865	37.0	37.0	37.0	37.0	37.0
2	35.2185	37.0	37.0	37.0	25.0	37.0
3	35.215	37.0	37.0	37.0	37.0	37.0
4	35.5115	37.0	37.0	37.0	37.0	37.0
5	35.645	37.0	37.0	37.0	37.0	37.0
6	35.493	37.0	37.0	37.0	37.0	37.0
7	35.4545	37.0	37.0	37.0	37.0	37.0
8	35.6095	37.0	37.0	37.0	37.0	37.0
9	35.376	37.0	37.0	37.0	37.0	37.0
10-14	35.572	37.0	37.0	37.0	37.0	37.0
15-19	35.4478	37.0	37.0	37.0	34.6	37.0
20-24	35.3356	37.0	37.0	37.0	32.2	37.0
25-29	35.0877	37.0	37.0	37.0	27.4	37.0
30-34	34.94435	37.0	37.0	37.0	25.0	37.0
35-39	34.698899999999995	37.0	37.0	37.0	25.0	37.0
40-44	34.6024	37.0	37.0	37.0	25.0	37.0
45-49	34.3577	37.0	37.0	37.0	25.0	37.0
50-54	33.9564	37.0	37.0	37.0	25.0	37.0
55-59	33.3511	37.0	37.0	37.0	13.8	37.0
60-64	32.8957	37.0	37.0	37.0	11.0	37.0
65-69	32.187	37.0	37.0	37.0	11.0	37.0
70-74	31.797500000000003	37.0	37.0	37.0	11.0	37.0
75-79	31.6858	37.0	29.8	37.0	11.0	37.0
80-84	31.5945	37.0	29.8	37.0	11.0	37.0
85-89	31.2913	37.0	25.0	37.0	11.0	37.0
90-94	30.973799999999994	37.0	25.0	37.0	11.0	37.0
95-99	30.571099999999994	37.0	25.0	37.0	11.0	37.0
100-104	30.0072	37.0	25.0	37.0	11.0	37.0
105-109	29.837200000000003	37.0	25.0	37.0	11.0	37.0
110-114	29.5409	37.0	25.0	37.0	11.0	37.0
115-119	29.206200000000003	37.0	22.2	37.0	11.0	37.0
120-124	28.9495	37.0	13.8	37.0	11.0	37.0
125-129	28.6432	37.0	11.0	37.0	11.0	37.0
130-134	28.602800000000002	37.0	11.0	37.0	11.0	37.0
135-139	28.7185	37.0	11.0	37.0	11.0	37.0
140-144	28.640099999999997	37.0	11.0	37.0	11.0	37.0
145-149	28.8841	37.0	11.0	37.0	11.0	37.0
150	28.9445	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	7.0
21	38.0
22	77.0
23	113.0
24	166.0
25	221.0
26	197.0
27	184.0
28	185.0
29	164.0
30	146.0
31	140.0
32	151.0
33	187.0
34	280.0
35	727.0
36	972.0
37	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.24112056028014	23.56178089044522	13.631815907953976	30.565282641320664
2	15.615615615615615	24.4994994994995	31.48148148148148	28.403403403403406
3	20.724999999999998	23.375	33.825	22.075
4	19.075	35.55	19.825	25.55
5	20.525	39.775	20.4	19.3
6	26.224999999999998	28.125	27.325	18.325
7	21.775	23.275000000000002	27.6	27.35
8	25.974999999999998	31.374999999999996	20.625	22.025
9	22.975	23.75	31.55	21.725
10-14	22.175	29.134999999999998	24.529999999999998	24.16
15-19	24.415	26.555	24.625	24.404999999999998
20-24	24.490000000000002	27.155	28.660000000000004	19.695
25-29	21.055	27.99	27.405	23.549999999999997
30-34	23.60118005900295	27.45137256862843	28.911445572278616	20.036001800090006
35-39	19.24	26.955000000000002	33.405	20.4
40-44	20.955	26.16	34.06	18.825
45-49	20.525	26.33	34.495	18.65
50-54	20.43	27.634999999999998	33.160000000000004	18.775
55-59	22.439999999999998	28.28	30.275000000000002	19.005
60-64	21.63	30.095	27.87	20.405
65-69	22.425	32.245000000000005	26.05	19.28
70-74	22.11	32.81	24.42	20.66
75-79	22.884999999999998	32.53	23.48	21.105
80-84	22.085	32.725	23.775	21.415
85-89	22.384999999999998	33.45	21.825	22.34
90-94	21.884999999999998	32.035000000000004	22.435	23.645
95-99	20.51	32.975	22.785	23.73
100-104	19.56	34.095	22.67	23.674999999999997
105-109	20.965	33.085	23.02	22.93
110-114	21.875	31.905	23.165	23.055
115-119	24.099999999999998	30.805	23.71	21.385
120-124	26.375	30.425	23.35	19.85
125-129	28.765	30.314999999999998	21.945	18.975
130-134	33.115	28.03	21.41	17.445
135-139	36.18	26.39	20.645	16.785
140-144	39.785	26.565	19.155	14.495
145-149	44.04940494049405	25.66756675667567	16.771677167716774	13.511351135113511
150	49.225	22.175	15.8	12.8
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.5
5	3.0
6	2.5
7	2.0
8	3.5
9	6.0
10	9.0
11	8.0
12	8.5
13	9.0
14	11.0
15	19.0
16	24.5
17	29.0
18	33.5
19	35.5
20	45.0
21	49.0
22	47.5
23	47.0
24	49.0
25	56.5
26	47.5
27	43.0
28	52.0
29	58.5
30	61.5
31	68.5
32	67.5
33	64.0
34	82.5
35	92.5
36	81.5
37	76.0
38	70.0
39	55.5
40	52.5
41	57.5
42	54.0
43	57.5
44	70.0
45	82.0
46	83.5
47	89.0
48	109.0
49	108.0
50	132.0
51	164.5
52	174.0
53	194.5
54	170.0
55	151.0
56	159.0
57	138.0
58	123.0
59	117.0
60	87.0
61	56.5
62	34.5
63	20.0
64	25.5
65	31.0
66	20.0
67	4.5
68	3.0
69	2.5
70	3.5
71	3.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.1
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.005
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.01
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.775000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68936678614098	50.025
2	11.549183592194346	14.499999999999998
3	3.8630027877339703	7.2749999999999995
4	1.7124651533253685	4.3
5	1.0752688172043012	3.375
6	0.9159697331740343	3.45
7	0.4778972520908005	2.1
8	0.11947431302270012	0.6
9	0.11947431302270012	0.675
>10	0.398247710075667	4.075
>50	0.0	0.0
>100	0.07964954201513341	9.625
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	234	5.8500000000000005	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	151	3.775	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	39	0.975	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	20	0.5	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.475	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	15	0.375	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.325	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	12	0.3	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.3	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
GTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAA	11	0.27499999999999997	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	11	0.27499999999999997	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	9	0.22499999999999998	No Hit
ATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.22499999999999998	No Hit
GCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTA	8	0.2	No Hit
GGCTTCTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATC	8	0.2	No Hit
GAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCC	8	0.2	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	7	0.17500000000000002	No Hit
CAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGT	7	0.17500000000000002	No Hit
GTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTCCTGGGGCC	7	0.17500000000000002	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	7	0.17500000000000002	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	7	0.17500000000000002	No Hit
CTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCA	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTAA	7	0.17500000000000002	No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CTCAGAGCTGAGCTGCACATCAATGTCAGGAGCCTGGTACTTGAGCCGTC	7	0.17500000000000002	No Hit
GGTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACG	7	0.17500000000000002	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	7	0.17500000000000002	No Hit
GCCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGG	6	0.15	No Hit
CAAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAACCA	6	0.15	No Hit
CGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAA	6	0.15	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	6	0.15	No Hit
CAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGC	6	0.15	No Hit
GTTTTTGGACATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAA	6	0.15	No Hit
CCACCGAGGTCGGCCGCAGCCCCAGGAAGCCAGAGTCCGCCTGGCTTCTC	6	0.15	No Hit
CTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGA	6	0.15	No Hit
CTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTAC	6	0.15	No Hit
TTCAAGAGAAAAGAACCAAGAAAGAAGAGGACCTTGGTCCAGAAGAAGTC	6	0.15	No Hit
GATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTT	6	0.15	No Hit
CTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACCAGACCAGC	6	0.15	No Hit
CATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT	6	0.15	No Hit
AGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTG	6	0.15	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	6	0.15	No Hit
GGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCAGGCACCTGGGAA	6	0.15	No Hit
CTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACG	6	0.15	No Hit
TCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGA	6	0.15	No Hit
CTGATGTGCGAGGATGTCTTTGGGAGCAGGGATCTTAGAATGATTCTCAA	6	0.15	No Hit
AAACGAAAAGCTCTTCTTCGTGGACACAGGATTCAAGAGAAAAGAACCAA	6	0.15	No Hit
CAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGA	6	0.15	No Hit
CGACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACAT	6	0.15	No Hit
GGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGACCGGTG	6	0.15	No Hit
GTTGGCATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAA	5	0.125	No Hit
CTCAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTC	5	0.125	No Hit
ATTGTCTTCCAGTTGGCATTTGTTTGAAATTGTATTGAAACAATTTAATG	5	0.125	No Hit
CCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTC	5	0.125	No Hit
GGTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCC	5	0.125	No Hit
GTTTATAACCTGGTCAATAGCTTGCATAGAGAAATTGAAGGTCATAAATT	5	0.125	No Hit
CCTGGGGCCCAGGTTTGGCCTTTGGTGTGGGAGGGCTAAGGAGTCGGGCC	5	0.125	No Hit
CTTGTCTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTGGCATTT	5	0.125	No Hit
CTTCTTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATC	5	0.125	No Hit
GTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAA	5	0.125	No Hit
GTTCCAGAAAAAATGCATCCTGACCAATCAAAGGCGTGTCCAGAGGGTTG	5	0.125	No Hit
ATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTC	5	0.125	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
GCTTGAACTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAG	5	0.125	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGTCCTTTA	5	0.125	No Hit
GTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAATACTTGGTTT	5	0.125	No Hit
CCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTAC	5	0.125	No Hit
CCACCAGCTTCACTTTGTATTTGCGCTTGAACTTGGCTCGTTCTCGGGGC	5	0.125	No Hit
GTTGTGGCCGCCTCACGCCTTTCTTCTTGGTCTGTTCCAGAAAAAATGCA	5	0.125	No Hit
GTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGT	5	0.125	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCAT	5	0.125	No Hit
AGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCC	5	0.125	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	5	0.125	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	5	0.125	No Hit
TTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGA	5	0.125	No Hit
ATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.037500000000000006	0.0	0.0	0.0	0.0
24-25	0.1125	0.0	0.0	0.0	0.0
26-27	0.125	0.0	0.0	0.0	0.0
28-29	0.3375	0.0	0.0	0.0	0.0
30-31	0.6625000000000001	0.0	0.0	0.0	0.0
32-33	1.2125	0.0	0.0	0.0	0.0
34-35	2.1125	0.0	0.0	0.0	0.0
36-37	2.6125	0.0	0.0	0.0	0.0
38-39	3.4	0.0	0.0	0.0	0.0
40-41	4.05	0.0	0.0	0.0	0.0
42-43	4.6875	0.0	0.0	0.0	0.0
44-45	5.35	0.0	0.0	0.0	0.0
46-47	6.275	0.0	0.0	0.0	0.0
48-49	7.1625	0.0	0.0	0.0	0.0
50-51	8.0625	0.0	0.0	0.0	0.0
52-53	9.1125	0.0	0.0	0.0	0.0
54-55	10.325	0.0	0.0	0.0	0.0
56-57	11.7	0.0	0.0	0.0	0.0
58-59	13.8	0.0	0.0	0.0	0.0
60-61	15.2625	0.0	0.0	0.0	0.0
62-63	16.825	0.0	0.0	0.0	0.0
64-65	18.1625	0.0	0.0	0.0	0.0
66-67	19.887500000000003	0.0	0.0	0.0	0.0
68-69	21.35	0.0	0.0	0.0	0.0
70-71	22.725	0.0	0.0	0.0	0.0
72-73	24.25	0.0	0.0	0.0	0.0
74-75	25.8125	0.0	0.0	0.0	0.0
76-77	27.425	0.0	0.0	0.0	0.0
78-79	29.2625	0.0	0.0	0.0	0.0
80-81	31.1	0.0	0.0	0.0	0.0
82-83	32.8625	0.0	0.0	0.0	0.0
84-85	34.400000000000006	0.0	0.0	0.0	0.0
86-87	36.05	0.0	0.0	0.0	0.0
88-89	37.8625	0.0	0.0	0.0	0.0
90-91	39.15	0.0	0.0	0.0	0.0
92-93	40.475	0.0	0.0	0.0	0.0
94-95	42.4	0.0	0.0	0.0	0.0
96-97	43.7875	0.0	0.0	0.0	0.0
98-99	45.3875	0.0	0.0	0.0	0.0
100-101	46.5625	0.0	0.0	0.0	0.0
102-103	47.2125	0.0	0.0	0.0	0.0
104-105	47.849999999999994	0.0	0.0	0.0	0.0
106-107	48.45	0.0	0.0	0.0	0.0
108-109	48.9875	0.0	0.0	0.0	0.0
110-111	49.6125	0.0	0.0	0.0	0.0
112-113	50.125	0.0	0.0	0.0	0.0
114-115	50.6	0.0	0.0	0.0	0.0
116-117	50.95	0.0	0.0	0.0	0.0
118-119	51.3125	0.0	0.0	0.0	0.0
120-121	51.65	0.0	0.0	0.0	0.0
122-123	52.0625	0.0	0.0	0.0	0.0
124-125	52.537499999999994	0.0	0.0	0.0	0.0
126-127	52.9375	0.0	0.0	0.0	0.0
128-129	53.1625	0.0	0.0	0.0	0.0
130-131	53.5375	0.0	0.0	0.0	0.0
132-133	53.849999999999994	0.0	0.0	0.0	0.0
134-135	54.25	0.0	0.0	0.0	0.0
136-137	54.6625	0.0	0.0	0.0	0.0
138	54.95	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	30	4.783942E-10	145.81013	1
CATTGCA	40	3.8799044E-9	107.99063	7
CTAATCA	40	3.8799044E-9	107.99063	2
TCATTGC	40	3.8799044E-9	107.99063	6
TTGCAAG	45	8.800271E-9	95.99167	9
AATCATT	45	8.800271E-9	95.99167	4
ATTGCAA	45	8.800271E-9	95.99167	8
TAATCAT	45	8.800271E-9	95.99167	3
ATCATTG	45	8.800271E-9	95.99167	5
AAGCAGT	60	1.083288E-5	36.452534	1
AGCAGTG	60	1.083288E-5	35.996876	2
CAGTGGT	65	4.41138E-4	33.227886	4
AGTGGTA	70	4.081436E-5	30.854464	5
TGGTATC	75	7.366495E-5	28.797499	7
GTATCAA	75	0.0013049653	28.797499	9
GTGGTAT	75	7.366495E-5	28.797499	6
CAAGCAG	50	2.2314052E-6	23.038	10-14
GCAAGCA	45	2.6262538E-5	22.398056	10-14
TGCAAGC	50	5.919679E-5	20.158249	10-14
TCAACGC	80	1.277325E-4	14.398751	20-24
>>END_MODULE
SRR14016358 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14016358_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.67825	37.0	37.0	37.0	25.0	37.0
2	34.417	37.0	37.0	37.0	25.0	37.0
3	34.9055	37.0	37.0	37.0	25.0	37.0
4	35.175	37.0	37.0	37.0	25.0	37.0
5	35.099	37.0	37.0	37.0	25.0	37.0
6	35.124	37.0	37.0	37.0	25.0	37.0
7	34.9955	37.0	37.0	37.0	25.0	37.0
8	35.2945	37.0	37.0	37.0	37.0	37.0
9	35.1885	37.0	37.0	37.0	37.0	37.0
10-14	35.2361	37.0	37.0	37.0	34.6	37.0
15-19	35.2222	37.0	37.0	37.0	32.2	37.0
20-24	35.2044	37.0	37.0	37.0	32.2	37.0
25-29	34.98180000000001	37.0	37.0	37.0	25.0	37.0
30-34	34.881600000000006	37.0	37.0	37.0	25.0	37.0
35-39	34.5659	37.0	37.0	37.0	25.0	37.0
40-44	34.4633	37.0	37.0	37.0	25.0	37.0
45-49	34.1059	37.0	37.0	37.0	25.0	37.0
50-54	33.606100000000005	37.0	37.0	37.0	19.4	37.0
55-59	32.8344	37.0	37.0	37.0	11.0	37.0
60-64	32.4483	37.0	37.0	37.0	11.0	37.0
65-69	31.713900000000002	37.0	29.8	37.0	11.0	37.0
70-74	31.305400000000002	37.0	25.0	37.0	11.0	37.0
75-79	31.2772	37.0	25.0	37.0	11.0	37.0
80-84	30.9495	37.0	25.0	37.0	11.0	37.0
85-89	30.562900000000003	37.0	25.0	37.0	11.0	37.0
90-94	30.389	37.0	25.0	37.0	11.0	37.0
95-99	30.0452	37.0	25.0	37.0	11.0	37.0
100-104	29.8471	37.0	25.0	37.0	11.0	37.0
105-109	29.6575	37.0	25.0	37.0	11.0	37.0
110-114	29.336599999999997	37.0	25.0	37.0	11.0	37.0
115-119	29.0448	37.0	16.6	37.0	11.0	37.0
120-124	28.9216	37.0	13.8	37.0	11.0	37.0
125-129	28.7339	37.0	13.8	37.0	11.0	37.0
130-134	28.394800000000004	37.0	11.0	37.0	11.0	37.0
135-139	28.4789	37.0	11.0	37.0	11.0	37.0
140-144	28.094399999999997	37.0	11.0	37.0	11.0	37.0
145-149	28.342699999999997	37.0	11.0	37.0	11.0	37.0
150	28.33	37.0	11.0	37.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	1.0
19	1.0
20	17.0
21	36.0
22	66.0
23	153.0
24	220.0
25	232.0
26	210.0
27	203.0
28	147.0
29	118.0
30	103.0
31	141.0
32	170.0
33	250.0
34	396.0
35	682.0
36	825.0
37	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.08227056764191	24.756189047261813	13.703425856464117	32.45811452863216
2	15.575	25.1	32.925	26.400000000000002
3	21.275	21.85	32.95	23.925
4	19.975	35.575	18.9	25.55
5	20.775	40.25	19.1	19.875
6	24.775	28.825	26.8	19.6
7	20.724999999999998	24.25	27.650000000000002	27.375
8	24.925	30.85	21.425	22.8
9	24.4	22.625	31.424999999999997	21.55
10-14	22.275	28.705000000000002	25.005	24.015
15-19	24.310000000000002	26.029999999999998	24.895	24.765
20-24	24.224999999999998	26.945000000000004	28.715000000000003	20.115
25-29	20.49	27.865000000000002	28.144999999999996	23.5
30-34	22.875	27.439999999999998	29.59	20.095
35-39	18.834999999999997	27.255000000000003	34.315	19.595000000000002
40-44	21.8	25.465	34.12	18.615000000000002
45-49	20.155	27.1	34.515	18.23
50-54	21.32	27.925	32.955	17.8
55-59	23.995	28.794999999999998	29.494999999999997	17.715
60-64	24.52	29.615000000000002	28.134999999999998	17.73
65-69	25.740000000000002	31.259999999999998	26.905	16.095000000000002
70-74	26.995	31.16	25.240000000000002	16.605
75-79	29.330000000000002	31.335	23.57	15.765
80-84	29.494999999999997	31.41	23.89	15.204999999999998
85-89	29.794999999999998	32.48	22.759999999999998	14.965
90-94	29.67	32.96	22.485	14.885000000000002
95-99	30.769999999999996	33.1	21.545	14.585
100-104	31.15	33.96	20.925	13.965
105-109	32.235	33.26	21.465	13.04
110-114	31.879999999999995	34.785	21.04	12.295
115-119	34.64	34.18	20.044999999999998	11.135
120-124	37.6	32.67	19.05	10.68
125-129	39.69	30.86	17.985	11.465
130-134	42.285000000000004	29.7	17.62	10.395
135-139	45.055	28.34	16.17	10.435
140-144	49.11	27.694999999999997	14.915000000000001	8.28
145-149	52.685	26.21	13.43	7.675
150	55.474999999999994	24.675	13.475000000000001	6.375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	1.0
4	2.5
5	3.0
6	4.5
7	7.0
8	5.5
9	3.5
10	6.0
11	6.0
12	10.0
13	15.5
14	11.5
15	11.5
16	18.5
17	31.0
18	37.0
19	35.5
20	36.5
21	44.5
22	46.5
23	40.0
24	50.0
25	55.5
26	54.5
27	61.0
28	62.0
29	59.5
30	59.0
31	58.5
32	61.5
33	75.5
34	80.0
35	76.0
36	75.5
37	67.0
38	62.0
39	64.0
40	62.0
41	55.0
42	56.0
43	61.5
44	71.5
45	79.5
46	85.5
47	102.0
48	108.0
49	110.5
50	132.0
51	151.0
52	170.5
53	184.0
54	162.5
55	144.0
56	136.5
57	129.0
58	135.0
59	130.5
60	99.5
61	73.0
62	46.5
63	23.5
64	29.0
65	30.5
66	14.5
67	5.5
68	4.0
69	3.0
70	3.0
71	1.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.5
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.12419293581466	53.400000000000006
2	10.748195974173946	14.149999999999999
3	3.7599696164071403	7.425
4	1.89897455374098	5.0
5	0.759589821496392	2.5
6	0.6836308393467527	2.7
7	0.2658564375237372	1.225
8	0.379794910748196	2.0
9	0.11393847322445878	0.675
>10	0.189897455374098	2.25
>50	0.0	0.0
>100	0.0759589821496392	8.674999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT	180	4.5	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	167	4.175	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTAAG	39	0.975	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.5	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.27499999999999997	No Hit
CTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT	10	0.25	No Hit
CTCAGAGCTGAGCTGCACATCAATGTCAGGAGCCTGGTACTTGAGCCGTC	10	0.25	No Hit
CTTTATTGAGGGGCGGGGCTCCGACATCTGCACAGCTACAACTGAATCTC	9	0.22499999999999998	No Hit
CCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGA	9	0.22499999999999998	No Hit
GTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCG	9	0.22499999999999998	No Hit
GTTGGCATTTGTTTGAAATTGTATTGAAACAATTTAATGAAAACATTAAA	8	0.2	No Hit
ATTTTTTCTGGAACAGACCAAGAAGAAAGGCGTGAGGCGGCCACAACGCC	8	0.2	No Hit
CTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTC	8	0.2	No Hit
TAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT	8	0.2	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGTAGAG	8	0.2	No Hit
ATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
GCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
TTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATCCTGT	8	0.2	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.2	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	8	0.2	No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.17500000000000002	No Hit
CTGGTGCTGATGTCCCTATGCCTTACGCAAAGGTCCTAGAAGACAACTCC	7	0.17500000000000002	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGGAC	7	0.17500000000000002	No Hit
CTGTTACCACCCGCAGCCATCTTCTTAAAGCCCCATGTACTCTGCGTTGA	7	0.17500000000000002	No Hit
CCCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGA	7	0.17500000000000002	No Hit
CGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAA	7	0.17500000000000002	No Hit
CCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAAT	7	0.17500000000000002	No Hit
CTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGG	6	0.15	No Hit
CGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGC	6	0.15	No Hit
GGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTC	6	0.15	No Hit
CACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCTTCTAT	6	0.15	No Hit
GAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCG	6	0.15	No Hit
TACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTA	6	0.15	No Hit
CCCAGACAACCCTCTGGACACGCCTTTGATTGGTCAGGATGCATTTTTTC	6	0.15	No Hit
GTTCAAGAGCTTCCAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCA	6	0.15	No Hit
CCCGAGAACGAGCCAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAG	6	0.15	No Hit
GCCAAGAAGCTCAGGCGAAAGGAGGAGTTATGGGAGAAACTGGCAAAGCA	6	0.15	No Hit
CAGAAGAGAAATATGATTGAGCCCCGAGAACGAGCCAAGTTCAAGCGCAA	6	0.15	No Hit
AATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT	6	0.15	No Hit
CTGGTGGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATG	6	0.15	No Hit
GTCACATTCTCCGAGACAGGTTCAAGAGCTTCCAGAAGAGAAATATGATT	6	0.15	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCC	6	0.15	No Hit
ATTCAAGAGAAAAGAACCAAGAAAGAAGAGGACCTTGGTCCAGAAGAAGT	6	0.15	No Hit
GTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAA	6	0.15	No Hit
ACCAGCTTCACTTTGTATTTGCGCTTGAACTTGGCTCGTTCTCGGGGCTC	6	0.15	No Hit
CTCAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTC	5	0.125	No Hit
GGAGAAGCGGGCCTACCGTGAGATTCAGTTGTAGCTGTGCAGATGTCGGA	5	0.125	No Hit
CCTCAATAAAGTTCTGTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.125	No Hit
ATCAAGCCTTCCCAGGTGCCTGCAGTGGAGGTGATTCCTGCAGGAGCCTC	5	0.125	No Hit
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGGGGAGGACA	5	0.125	No Hit
CAAGTTCAAGCGCAAATACAAAGTGAAGCTGGTGGAGAAGCGGGCCTACC	5	0.125	No Hit
GTCCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGA	5	0.125	No Hit
CTTCTTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATC	5	0.125	No Hit
ACTCTTCTGACTTCCATGGATTTTCGGCAAGATACTCAATACACATAAAA	5	0.125	No Hit
TTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
CCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTTAAAGCC	5	0.125	No Hit
ATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGG	5	0.125	No Hit
CTCTTCAGCAGCCCGGAAGCCTGCCGCAAGGGTCCCCGCACCAGACCAGC	5	0.125	No Hit
CAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCAGGC	5	0.125	No Hit
GTTAGAGGTCGACCAGTTCCTGGAAGACGTCCGGCTACAGGAGCGCACGA	5	0.125	No Hit
GCTTGAACTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAG	5	0.125	No Hit
CTTACGGGTTGACCTTGCCCTTGAGAATCATTCTAAGATCCCTGCTCCCA	5	0.125	No Hit
GTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGT	5	0.125	No Hit
CTTGAACTTGGCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGC	5	0.125	No Hit
CTCGAGCCGCGCTTCTCACCGTCCCTGTTACCACCCGCAGCCATCTTCTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.037500000000000006	0.0	0.0	0.0	0.0
24-25	0.1125	0.0	0.0	0.0	0.0
26-27	0.125	0.0	0.0	0.0	0.0
28-29	0.35	0.0	0.0	0.0	0.0
30-31	0.675	0.0	0.0	0.0	0.0
32-33	1.2625000000000002	0.0	0.0	0.0	0.0
34-35	2.1375	0.0	0.0	0.0	0.0
36-37	2.6125	0.0	0.0	0.0	0.0
38-39	3.4	0.0	0.0	0.0	0.0
40-41	4.075	0.0	0.0	0.0	0.0
42-43	4.7125	0.0	0.0	0.0	0.0
44-45	5.362500000000001	0.0	0.0	0.0	0.0
46-47	6.2125	0.0	0.0	0.0	0.0
48-49	7.0	0.0	0.0	0.0	0.0
50-51	7.875	0.0	0.0	0.0	0.0
52-53	8.975	0.0	0.0	0.0	0.0
54-55	10.1875	0.0	0.0	0.0	0.0
56-57	11.537500000000001	0.0	0.0	0.0	0.0
58-59	13.575	0.0	0.0	0.0	0.0
60-61	14.9875	0.0	0.0	0.0	0.0
62-63	16.4625	0.0	0.0	0.0	0.0
64-65	17.775	0.0	0.0	0.0	0.0
66-67	19.475	0.0	0.0	0.0	0.0
68-69	20.825	0.0	0.0	0.0	0.0
70-71	22.175	0.0	0.0	0.0	0.0
72-73	23.6625	0.0	0.0	0.0	0.0
74-75	25.237499999999997	0.0	0.0	0.0	0.0
76-77	26.8125	0.0	0.0	0.0	0.0
78-79	28.549999999999997	0.0	0.0	0.0	0.0
80-81	30.4	0.0	0.0	0.0	0.0
82-83	32.1625	0.0	0.0	0.0	0.0
84-85	33.6875	0.0	0.0	0.0	0.0
86-87	35.400000000000006	0.0	0.0	0.0	0.0
88-89	37.150000000000006	0.0	0.0	0.0	0.0
90-91	38.3875	0.0	0.0	0.0	0.0
92-93	39.6875	0.0	0.0	0.0	0.0
94-95	41.6	0.0	0.0	0.0	0.0
96-97	43.025	0.0	0.0	0.0	0.0
98-99	44.6375	0.0	0.0	0.0	0.0
100-101	45.875	0.0	0.0	0.0	0.0
102-103	46.4875	0.0	0.0	0.0	0.0
104-105	47.099999999999994	0.0	0.0	0.0	0.0
106-107	47.675	0.0	0.0	0.0	0.0
108-109	48.2	0.0	0.0	0.0	0.0
110-111	48.825	0.0	0.0	0.0	0.0
112-113	49.3375	0.0	0.0	0.0	0.0
114-115	49.8	0.0	0.0	0.0	0.0
116-117	50.1625	0.0	0.0	0.0	0.0
118-119	50.525	0.0	0.0	0.0	0.0
120-121	50.9	0.0	0.0	0.0	0.0
122-123	51.35	0.0	0.0	0.0	0.0
124-125	51.837500000000006	0.0	0.0	0.0	0.0
126-127	52.2375	0.0	0.0	0.0	0.0
128-129	52.4125	0.0	0.0	0.0	0.0
130-131	52.775000000000006	0.0	0.0	0.0	0.0
132-133	53.099999999999994	0.0	0.0	0.0	0.0
134-135	53.525	0.0	0.0	0.0	0.0
136-137	53.95	0.0	0.0	0.0	0.0
138	54.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAATC	30	5.2386895E-10	144.0	1
CTAATCA	30	5.2386895E-10	144.0	2
TAATCAT	30	5.2386895E-10	144.0	3
AATCATT	35	1.5315891E-9	123.428566	4
TCATTGC	40	3.8780854E-9	108.0	6
CATTGCA	35	2.3845314E-7	102.85714	7
ATCATTG	50	1.828812E-8	86.399994	5
TTGCAAG	35	3.1411873E-5	82.28571	9
ATTGCAA	35	3.1411873E-5	82.28571	8
AAGCAGT	60	5.887756E-6	60.0	1
AGCAGTG	70	1.46842085E-5	51.42857	2
GCAGTGG	70	1.46842085E-5	51.42857	3
AGTGGTA	75	2.2092254E-5	47.999996	5
CAGTGGT	75	2.2092254E-5	47.999996	4
TGGTATC	80	3.236105E-5	45.0	7
GTATCAA	80	3.236105E-5	45.0	9
GTGGTAT	80	3.236105E-5	45.0	6
GGTATCA	80	3.236105E-5	45.0	8
GAGTACA	35	0.0036813593	20.571428	3
CAAGCAG	40	0.007966741	18.0	5
>>END_MODULE
2023-05-11T07:20:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:20:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:24 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000081.6 as a RefSeq or as a WGS
2023-05-11T07:21:24 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016358.sra
2023-05-11T07:21:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:26 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000076.6 as a RefSeq or as a WGS
2023-05-11T07:21:26 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016358.sra
2023-05-11T07:21:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:30 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000067.6 as a RefSeq or as a WGS
2023-05-11T07:21:30 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016358.sra
2023-05-11T07:21:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:30 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000073.6 as a RefSeq or as a WGS
2023-05-11T07:21:30 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016358.sra
2023-05-11T07:21:30 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000069.6 as a RefSeq or as a WGS
2023-05-11T07:21:30 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016358.sra
2023-05-11T07:21:31 fastq-dump.3.0.1 warn: database incorrect while opening manager within database module - can't open NC_000067.6 as a RefSeq or as a WGS
2023-05-11T07:21:31 fastq-dump.3.0.1 err: database incorrect while opening manager within database module - failed SRR14016358.sra
2023-05-11T07:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:35 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:51 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
SRR ids: ['SRR14016358.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xn2h92ot
SRR14016358.sra spots: 33834399
blocks: [[1, 528662], [528663, 1057324], [1057325, 1585986], [1585987, 2114648], [2114649, 2643310], [2643311, 3171972], [3171973, 3700634], [3700635, 4229296], [4229297, 4757958], [4757959, 5286620], [5286621, 5815282], [5815283, 6343944], [6343945, 6872606], [6872607, 7401268], [7401269, 7929930], [7929931, 8458592], [8458593, 8987254], [8987255, 9515916], [9515917, 10044578], [10044579, 10573240], [10573241, 11101902], [11101903, 11630564], [11630565, 12159226], [12159227, 12687888], [12687889, 13216550], [13216551, 13745212], [13745213, 14273874], [14273875, 14802536], [14802537, 15331198], [15331199, 15859860], [15859861, 16388522], [16388523, 16917184], [16917185, 17445846], [17445847, 17974508], [17974509, 18503170], [18503171, 19031832], [19031833, 19560494], [19560495, 20089156], [20089157, 20617818], [20617819, 21146480], [21146481, 21675142], [21675143, 22203804], [22203805, 22732466], [22732467, 23261128], [23261129, 23789790], [23789791, 24318452], [24318453, 24847114], [24847115, 25375776], [25375777, 25904438], [25904439, 26433100], [26433101, 26961762], [26961763, 27490424], [27490425, 28019086], [28019087, 28547748], [28547749, 29076410], [29076411, 29605072], [29605073, 30133734], [30133735, 30662396], [30662397, 31191058], [31191059, 31719720], [31719721, 32248382], [32248383, 32777044], [32777045, 33305706], [33305707, 33834399]]
fastq-dump error! exit code: 3
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3

=============================================================
An error occurred during processing.
A report was generated into the file '/root/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.
=============================================================

fastq-dump quit with error code 3
SRR14016358 file size 353032
SRR14016358 completed basic pipeline successfully
2023-05-11T07:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 64 -o SRR14016358 SRR14016358_1.fastq SRR14016358_2.fastq
Input file:	SRR14016358_1.fastq
Paired file:	SRR14016358_2.fastq
trimmed:	SRR14016358-trimmed-pair1.fastq, SRR14016358-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 07:21:57 2023 >> started

Thu May 11 07:21:59 2023 >> done (2.260s)
1057324 read pairs processed; of these:
   1344 ( 0.13%) short read pairs filtered out after trimming by size control
    693 ( 0.07%) empty read pairs filtered out after trimming by size control
1055287 (99.81%) read pairs available; of these:
 589459 (55.86%) trimmed read pairs available after processing
 465828 (44.14%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      8	  0.00%
 19	      5	  0.00%
 20	     87	  0.01%
 21	    102	  0.01%
 22	     94	  0.01%
 23	    100	  0.01%
 24	    101	  0.01%
 25	    283	  0.03%
 26	     38	  0.00%
 27	    285	  0.03%
 28	    638	  0.06%
 29	   1553	  0.15%
 30	   1836	  0.17%
 31	   2826	  0.27%
 32	   5450	  0.52%
 33	   2828	  0.27%
 34	   2816	  0.27%
 35	   4407	  0.42%
 36	   1823	  0.17%
 37	   2691	  0.26%
 38	   3084	  0.29%
 39	   4857	  0.46%
 40	   6704	  0.64%
 41	  14463	  1.37%
 42	  24864	  2.36%
 43	   7666	  0.73%
 44	   6686	  0.63%
 45	   5737	  0.54%
 46	   5260	  0.50%
 47	   5924	  0.56%
 48	   5717	  0.54%
 49	   6634	  0.63%
 50	   5860	  0.56%
 51	   6621	  0.63%
 52	   6734	  0.64%
 53	   6569	  0.62%
 54	   6567	  0.62%
 55	   6505	  0.62%
 56	   6470	  0.61%
 57	   6815	  0.65%
 58	   6441	  0.61%
 59	   6753	  0.64%
 60	   6172	  0.58%
 61	   5831	  0.55%
 62	   5765	  0.55%
 63	   5738	  0.54%
 64	   5859	  0.56%
 65	   6051	  0.57%
 66	   5950	  0.56%
 67	   5845	  0.55%
 68	   5686	  0.54%
 69	   5740	  0.54%
 70	   5676	  0.54%
 71	   5630	  0.53%
 72	   5890	  0.56%
 73	   5674	  0.54%
 74	   5875	  0.56%
 75	   5500	  0.52%
 76	   5641	  0.53%
 77	   5469	  0.52%
 78	   5452	  0.52%
 79	   5366	  0.51%
 80	   5508	  0.52%
 81	   5304	  0.50%
 82	   5269	  0.50%
 83	   5143	  0.49%
 84	   4991	  0.47%
 85	   5136	  0.49%
 86	   5095	  0.48%
 87	   4990	  0.47%
 88	   5014	  0.48%
 89	   4881	  0.46%
 90	   4927	  0.47%
 91	   4849	  0.46%
 92	   4886	  0.46%
 93	   4674	  0.44%
 94	   4790	  0.45%
 95	   4658	  0.44%
 96	   4606	  0.44%
 97	   4586	  0.43%
 98	   4726	  0.45%
 99	   4444	  0.42%
100	   4238	  0.40%
101	   4366	  0.41%
102	   4494	  0.43%
103	   4318	  0.41%
104	   4191	  0.40%
105	   4205	  0.40%
106	   4112	  0.39%
107	   4282	  0.41%
108	   4081	  0.39%
109	   4026	  0.38%
110	   4250	  0.40%
111	   3960	  0.38%
112	   4126	  0.39%
113	   4057	  0.38%
114	   4111	  0.39%
115	   4135	  0.39%
116	   3954	  0.37%
117	   3930	  0.37%
118	   3981	  0.38%
119	   3949	  0.37%
120	   3785	  0.36%
121	   3725	  0.35%
122	   3741	  0.35%
123	   3749	  0.36%
124	   3588	  0.34%
125	   3765	  0.36%
126	   3543	  0.34%
127	   3520	  0.33%
128	   3508	  0.33%
129	   3556	  0.34%
130	   3598	  0.34%
131	   3521	  0.33%
132	   3426	  0.32%
133	   3497	  0.33%
134	   3376	  0.32%
135	   3391	  0.32%
136	   3479	  0.33%
137	   3376	  0.32%
138	   3461	  0.33%
139	   3248	  0.31%
140	   3401	  0.32%
141	   3167	  0.30%
142	   3269	  0.31%
143	   3198	  0.30%
144	   3053	  0.29%
145	   3254	  0.31%
146	   3069	  0.29%
147	   3027	  0.29%
148	   3213	  0.30%
149	   3000	  0.28%
150	 465828	 44.14%
1055287 reads passed initial QC
2023-05-11T07:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:21:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file


criterion=sequence-density
sequence-density=5.26
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=33
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=37.08
fanout-score-rank=1
prefix-density=0.55
prefix-fanout=1.1
sequence=AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCA
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file


criterion=sequence-density
sequence-density=5.69
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=34
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=31
fanout-score=33.88
fanout-score-rank=1
prefix-density=0.53
prefix-fanout=1.1
sequence=AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 818: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 819: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 64 -x GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG -y GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG -o SRR14016358 SRR14016358_1.fastq SRR14016358_2.fastq
Input file:	SRR14016358_1.fastq
Paired file:	SRR14016358_2.fastq
trimmed:	SRR14016358-trimmed-pair1.fastq, SRR14016358-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG
-- paired 3' end adapter sequence (-y):	GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	32
Thu May 11 07:22:16 2023 >> started

Thu May 11 07:22:18 2023 >> done (1.485s)
703525 read pairs processed; of these:
    22 ( 0.00%) short read pairs filtered out after trimming by size control
 53058 ( 7.54%) empty read pairs filtered out after trimming by size control
650445 (92.46%) read pairs available; of these:
     5 ( 0.00%) trimmed read pairs available after processing
650440 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     4	  0.00%
 19	     4	  0.00%
 20	    54	  0.01%
 21	    46	  0.01%
 22	    75	  0.01%
 23	    72	  0.01%
 24	    60	  0.01%
 25	   187	  0.03%
 26	    25	  0.00%
 27	   200	  0.03%
 28	   410	  0.06%
 29	   868	  0.13%
 30	  1078	  0.17%
 31	  1262	  0.19%
 32	  1429	  0.22%
 33	  1759	  0.27%
 34	  1603	  0.25%
 35	  1378	  0.21%
 36	  1239	  0.19%
 37	  1717	  0.26%
 38	  2050	  0.32%
 39	  3153	  0.48%
 40	  4354	  0.67%
 41	  9614	  1.48%
 42	 13437	  2.07%
 43	  5009	  0.77%
 44	  4391	  0.68%
 45	  3818	  0.59%
 46	  3480	  0.54%
 47	  3888	  0.60%
 48	  3788	  0.58%
 49	  4050	  0.62%
 50	  3633	  0.56%
 51	  4125	  0.63%
 52	  4116	  0.63%
 53	  3956	  0.61%
 54	  4117	  0.63%
 55	  4172	  0.64%
 56	  4199	  0.65%
 57	  4304	  0.66%
 58	  3931	  0.60%
 59	  4164	  0.64%
 60	  3686	  0.57%
 61	  3710	  0.57%
 62	  3496	  0.54%
 63	  3589	  0.55%
 64	  3634	  0.56%
 65	  3731	  0.57%
 66	  3619	  0.56%
 67	  3564	  0.55%
 68	  3539	  0.54%
 69	  3659	  0.56%
 70	  3514	  0.54%
 71	  3639	  0.56%
 72	  3708	  0.57%
 73	  3622	  0.56%
 74	  3777	  0.58%
 75	  3480	  0.54%
 76	  3640	  0.56%
 77	  3525	  0.54%
 78	  3324	  0.51%
 79	  3355	  0.52%
 80	  3450	  0.53%
 81	  3387	  0.52%
 82	  3365	  0.52%
 83	  3351	  0.52%
 84	  3244	  0.50%
 85	  3041	  0.47%
 86	  3207	  0.49%
 87	  3195	  0.49%
 88	  3236	  0.50%
 89	  3048	  0.47%
 90	  3222	  0.50%
 91	  3064	  0.47%
 92	  3031	  0.47%
 93	  2896	  0.45%
 94	  2952	  0.45%
 95	  2894	  0.44%
 96	  2958	  0.45%
 97	  2879	  0.44%
 98	  2940	  0.45%
 99	  2774	  0.43%
100	  2618	  0.40%
101	  2835	  0.44%
102	  2756	  0.42%
103	  2704	  0.42%
104	  2643	  0.41%
105	  2507	  0.39%
106	  2586	  0.40%
107	  2636	  0.41%
108	  2479	  0.38%
109	  2511	  0.39%
110	  2679	  0.41%
111	  2455	  0.38%
112	  2616	  0.40%
113	  2514	  0.39%
114	  2472	  0.38%
115	  2464	  0.38%
116	  2507	  0.39%
117	  2511	  0.39%
118	  2381	  0.37%
119	  2371	  0.36%
120	  2344	  0.36%
121	  2296	  0.35%
122	  2300	  0.35%
123	  2308	  0.35%
124	  2165	  0.33%
125	  2294	  0.35%
126	  2159	  0.33%
127	  2282	  0.35%
128	  2240	  0.34%
129	  2196	  0.34%
130	  2194	  0.34%
131	  2112	  0.32%
132	  2125	  0.33%
133	  2250	  0.35%
134	  2072	  0.32%
135	  2107	  0.32%
136	  2179	  0.34%
137	  2090	  0.32%
138	  2082	  0.32%
139	  1981	  0.30%
140	  2108	  0.32%
141	  1971	  0.30%
142	  2050	  0.32%
143	  1935	  0.30%
144	  1816	  0.28%
145	  1950	  0.30%
146	  1890	  0.29%
147	  1856	  0.29%
148	  1934	  0.30%
149	  1862	  0.29%
150	286888	 44.11%
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra
2023-05-11T07:22:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2023-05-11T07:22:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file


criterion=sequence-density
sequence-density=3.27
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=35
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=28.72
fanout-score-rank=1
prefix-density=0.43
prefix-fanout=1.1
sequence=AATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCA
Read 528662 spots for SRR14016358.sra
Written 528662 spots for SRR14016358.sra


criterion=sequence-density
sequence-density=3.47
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=34
prefix-density=0.00
prefix-fanout=1.0
sequence=GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACATGGG


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=21
fanout-score=73.87
fanout-score-rank=1
prefix-density=8.88
prefix-fanout=1.0
sequence=ACGCAGAGTACT
SRR14016358 testing PE reads STAR mapping to Ensembl genome
Read 528693 spots for SRR14016358.sra
Written 528693 spots for SRR14016358.sra
                                 Started job on |	May 11 07:23:48
                             Started mapping on |	May 11 07:23:49
                                    Finished on |	May 11 07:24:14
       Mapping speed, Million of reads per hour |	144.32

                          Number of input reads |	1002207
                      Average input read length |	224
                                    UNIQUE READS:
                   Uniquely mapped reads number |	464556
                        Uniquely mapped reads % |	46.35%
                          Average mapped length |	216.77
                       Number of splices: Total |	446951
            Number of splices: Annotated (sjdb) |	442748
                       Number of splices: GT/AG |	435367
                       Number of splices: GC/AG |	10107
                       Number of splices: AT/AC |	440
               Number of splices: Non-canonical |	1037
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.51
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.22
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	337790
             % of reads mapped to multiple loci |	33.70%
        Number of reads mapped to too many loci |	2215
             % of reads mapped to too many loci |	0.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	19.65%
                     % of reads unmapped: other |	0.07%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	199861	199861	199861
N_multimapping	337790	337790	337790
N_noFeature	38830	244386	245106
N_ambiguous	26126	6470	6017
UnstrandedReadsAssigned:399600 PositiveStrandReadsAssigned:213700 NegativeStrandReadsAssigned:213433
Dataset is classified unstranded
MeadianReadLen=131 20thPercentileLength=63 echo kmer=59
SRR14016358 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR14016358-trimmed-pair1.fastq
                             SRR14016358-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,002,207 reads, 745,521 reads pseudoaligned
[quant] estimated average fragment length: 123.248
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 803 rounds

 109283 SRR14016358.ke.tsv
  52636 SRR14016358.se.tsv
 161919 total
==> SRR14016358.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	2	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	2	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR14016358.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR14016358 completed mapping pipeline successfully
