Starting /dee2/code/volunteer_pipeline.sh SRR24820864
    current disk space = 1417842780
    free memory = 195011492 
SRR24820864 SRAfilesize
b5e7710b3c01e2d6df39785bc8afdcf7  SRR24820864.sra
SRR24820864.sra file validated
SRR24820864 is paired end
SRR24820864 is conventional basespace
SRR24820864 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24820864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9435	34.0	34.0	34.0	34.0	34.0
2	33.0195	34.0	34.0	34.0	34.0	34.0
3	32.914	34.0	34.0	34.0	34.0	34.0
4	33.2185	34.0	34.0	34.0	34.0	34.0
5	33.249	34.0	34.0	34.0	34.0	34.0
6	33.29	34.0	34.0	34.0	34.0	34.0
7	33.337	34.0	34.0	34.0	34.0	34.0
8	33.276	34.0	34.0	34.0	34.0	34.0
9	33.11	34.0	34.0	34.0	34.0	34.0
10	33.25	34.0	34.0	34.0	34.0	34.0
11	33.2945	34.0	34.0	34.0	34.0	34.0
12	33.268	34.0	34.0	34.0	34.0	34.0
13	33.3065	34.0	34.0	34.0	34.0	34.0
14	33.271	34.0	34.0	34.0	34.0	34.0
15	33.249	34.0	34.0	34.0	34.0	34.0
16	33.3125	34.0	34.0	34.0	34.0	34.0
17	33.3575	34.0	34.0	34.0	34.0	34.0
18	33.28	34.0	34.0	34.0	34.0	34.0
19	33.332	34.0	34.0	34.0	34.0	34.0
20	33.2615	34.0	34.0	34.0	34.0	34.0
21	33.1725	34.0	34.0	34.0	34.0	34.0
22	33.2935	34.0	34.0	34.0	34.0	34.0
23	33.116	34.0	34.0	34.0	34.0	34.0
24	33.14	34.0	34.0	34.0	34.0	34.0
25	33.1125	34.0	34.0	34.0	34.0	34.0
26	33.2715	34.0	34.0	34.0	34.0	34.0
27	33.316	34.0	34.0	34.0	34.0	34.0
28	33.2215	34.0	34.0	34.0	34.0	34.0
29	33.2035	34.0	34.0	34.0	34.0	34.0
30	33.271	34.0	34.0	34.0	34.0	34.0
31	33.161	34.0	34.0	34.0	34.0	34.0
32	33.2915	34.0	34.0	34.0	34.0	34.0
33	33.2	34.0	34.0	34.0	34.0	34.0
34	33.1835	34.0	34.0	34.0	34.0	34.0
35	33.175	34.0	34.0	34.0	34.0	34.0
36	33.2225	34.0	34.0	34.0	34.0	34.0
37	33.1565	34.0	34.0	34.0	34.0	34.0
38	33.2035	34.0	34.0	34.0	34.0	34.0
39	33.1695	34.0	34.0	34.0	34.0	34.0
40	33.174	34.0	34.0	34.0	34.0	34.0
41	32.963	34.0	34.0	34.0	34.0	34.0
42	33.155	34.0	34.0	34.0	34.0	34.0
43	33.084	34.0	34.0	34.0	34.0	34.0
44	33.1545	34.0	34.0	34.0	34.0	34.0
45	33.0995	34.0	34.0	34.0	34.0	34.0
46	32.983	34.0	34.0	34.0	34.0	34.0
47	33.037	34.0	34.0	34.0	34.0	34.0
48	33.179	34.0	34.0	34.0	34.0	34.0
49	33.016	34.0	34.0	34.0	34.0	34.0
50	32.9845	34.0	34.0	34.0	34.0	34.0
51	32.7835	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	3.0
24	5.0
25	12.0
26	24.0
27	52.0
28	74.0
29	61.0
30	97.0
31	129.0
32	337.0
33	2088.0
34	1118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.75	12.5	2.625	37.125
2	18.224999999999998	10.925	42.325	28.525
3	16.825000000000003	14.325	27.525	41.325
4	21.099999999999998	27.1	22.175	29.625
5	26.275	30.049999999999997	23.849999999999998	19.825
6	23.925	25.85	24.925	25.3
7	18.525	26.375	39.625	15.475
8	19.925	21.6	34.875	23.599999999999998
9	19.125	17.849999999999998	37.175000000000004	25.85
10	22.375	31.8	26.150000000000002	19.675
11	25.924999999999997	27.725	22.1	24.25
12	22.975	22.325	28.799999999999997	25.900000000000002
13	23.125	24.575	26.75	25.55
14	24.4	26.05	25.174999999999997	24.375
15	23.925	23.25	26.375	26.450000000000003
16	23.875	25.825	23.674999999999997	26.625
17	23.1	27.650000000000002	24.4	24.85
18	24.5	24.4	27.900000000000002	23.200000000000003
19	27.250000000000004	24.9	23.45	24.4
20	23.1	26.525	25.85	24.525
21	22.975	26.150000000000002	25.924999999999997	24.95
22	24.925	24.4	27.200000000000003	23.474999999999998
23	24.05	24.9	26.5	24.55
24	23.775	23.875	27.925	24.425
25	24.05	23.674999999999997	28.599999999999998	23.674999999999997
26	21.85	23.974999999999998	28.4	25.775
27	22.6	24.275	27.450000000000003	25.674999999999997
28	25.624999999999996	25.650000000000002	25.3	23.425
29	25.45	26.775	25.650000000000002	22.125
30	23.05	22.900000000000002	26.700000000000003	27.35
31	24.505632040050063	26.458072590738425	25.20650813516896	23.829787234042556
32	25.75	25.324999999999996	26.174999999999997	22.75
33	22.8	23.200000000000003	27.775	26.224999999999998
34	25.825	25.074999999999996	24.125	24.975
35	25.0	23.400000000000002	28.075	23.525
36	21.525	25.45	27.150000000000002	25.874999999999996
37	25.95	27.224999999999998	23.95	22.875
38	23.225	22.3	29.25	25.224999999999998
39	20.75	25.074999999999996	26.275	27.900000000000002
40	23.275000000000002	26.150000000000002	24.175	26.400000000000002
41	21.655413853463365	24.90622655663916	26.131532883220803	27.306826706676667
42	21.45	22.225	27.175	29.15
43	22.825	26.224999999999998	24.474999999999998	26.474999999999998
44	24.0	26.150000000000002	25.05	24.8
45	23.724999999999998	26.775	25.275	24.224999999999998
46	24.20605151287822	27.656914228557138	23.10577644411103	25.03125781445361
47	25.05	25.974999999999998	24.575	24.4
48	22.5	24.85	26.05	26.6
49	22.15	28.675	23.25	25.924999999999997
50	21.2	28.175	25.55	25.074999999999996
51	23.816679188580014	25.043826696719258	25.66992236413724	25.469571750563485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	1.0
19	4.0
20	7.0
21	5.5
22	4.0
23	2.5
24	1.0
25	4.5
26	9.0
27	10.0
28	18.0
29	26.0
30	26.0
31	26.0
32	25.5
33	25.0
34	41.5
35	58.0
36	95.5
37	133.0
38	159.5
39	186.0
40	255.0
41	324.0
42	333.0
43	342.0
44	323.0
45	304.0
46	367.5
47	431.0
48	393.0
49	355.0
50	343.5
51	332.0
52	340.0
53	348.0
54	293.0
55	238.0
56	215.0
57	192.0
58	203.5
59	215.0
60	189.0
61	163.0
62	144.0
63	125.0
64	102.5
65	80.0
66	64.0
67	48.0
68	30.0
69	12.0
70	7.0
71	2.0
72	1.0
73	0.0
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.125
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.025
42	0.0
43	0.0
44	0.0
45	0.0
46	0.025
47	0.0
48	0.0
49	0.0
50	0.0
51	0.17500000000000002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.05224429727741	55.074999999999996
2	10.338484179543782	14.05
3	3.642384105960265	7.425
4	1.545253863134658	4.2
5	0.9933774834437087	3.375
6	0.515084621044886	2.1
7	0.5518763796909493	2.625
8	0.22075055187637968	1.2
9	0.18395879323031641	1.125
>10	0.9565857247976454	8.825
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCAGCATGGATTGCAATAAATGGGTCCTCAGCAGAGTTACTAATGGAAAA	29	0.7250000000000001	No Hit
GTCCAGTGCAATTGCCATCGACCTTATCTCCAGAGATGACATCACAGTATG	24	0.6	No Hit
CTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAGTTTGAAAAAG	21	0.525	No Hit
CTTGGCAATATCAACCCAGCGCCACTCGAACAGGTGGACAATAGCAGTCCT	20	0.5	No Hit
GTCCAATCCAGTCATTCTGATCTTTTCCATTCTGGAAATTTCTATTCCAAC	19	0.475	No Hit
CCTGATTTGACGCCATCTGTGTTCACAGACCCAGTCATTGCCACAAGTAGT	17	0.42500000000000004	No Hit
GCCATTTCCAAAGTACTCACTACCTTTAATTGCCTCACCACCCAGATCAAT	16	0.4	No Hit
GTCACTTCTTTTGTTACTCCATTGTTATTGGGTGGTCCAATCCAGTCATTC	14	0.35000000000000003	No Hit
GTGCAATTGCCATCGACCTTATCTCCAGAGATGACATCACAGTATGTGCCA	13	0.325	No Hit
GTCCTCAGCAGAGTTACTAATGGAAAAGTGAGCTTTGCCATCACTGCCAAC	12	0.3	No Hit
CTCCATTGTTATTGGGTGGTCCAATCCAGTCATTCTGATCTTTTCCATTCT	12	0.3	No Hit
GCCAGCAGGAAGACCAGTCTGTAAAGTGGCTGACAAAGCCCAGTCATCATT	12	0.3	No Hit
GCCACCTTGGTACGAACATAATCTTTCTCAAGTGCAAGATCCAGAAGGCCA	11	0.27499999999999997	No Hit
GCCATCACTGCCAACATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTT	11	0.27499999999999997	No Hit
GCCAACATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTTATCTCCAGA	11	0.27499999999999997	No Hit
CTTAGGAGCTAAGTATCGCTCACATTCCTTGGCAATATCAACCCAGCGCCA	11	0.27499999999999997	No Hit
GTTTCCTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAGTTTGA	10	0.25	No Hit
GTCTGCTTCCTTGGGAGAACCATTTTGTATTGAGATTATGCAGTTTGTCCA	10	0.25	No Hit
CTCCAGGCCACATGTGCTTAGCAGCATCAAGTCTGAACCCTGCTACTCCAA	10	0.25	No Hit
CTCTGTTTCCTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAGT	10	0.25	No Hit
GCCACATGTGCTTAGCAGCATCAAGTCTGAACCCTGCTACTCCAATGTCAA	10	0.25	No Hit
CTCCATTACATTTATTATCGTTAAAGTCCCAAGCAGAGTATGGAACTGCTG	10	0.25	No Hit
CCTCTGTTTCCTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAG	10	0.25	No Hit
CTCACATTCCTTGGCAATATCAACCCAGCGCCACTCGAACAGGTGGACAAT	10	0.25	No Hit
GCCATTTTATACATTCTAGCATCCCAGAATGTCAGGATGGATGATCCTCCA	10	0.25	No Hit
GTTATGAACTACAACGTTTTCATTGGGTGGAGAGACCTGCACCCCTCCAAA	10	0.25	No Hit
CCCCAACCTTCTCCCCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCAC	9	0.22499999999999998	No Hit
GCACCTTGTCACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCA	9	0.22499999999999998	No Hit
GCCAAGTTTTGCACCATACTTGAATTCTGTCACACGGCCATTTCCAAAGTA	9	0.22499999999999998	No Hit
CAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTTGCGGATAACTGTG	9	0.22499999999999998	No Hit
CTTTAATTGCCTCACCACCCAGATCAATGACCTCTTGGAAAATGAAAGGTC	9	0.22499999999999998	No Hit
GCCACAAGTAGTGTCTGCATTAATGGTCACTTCTTTTGTTACTCCATTGTT	8	0.2	No Hit
CACCAGTTTGAAAAAGGCTGACCATTGACCACATTCCTGAAGGCAACCATG	8	0.2	No Hit
GGTTCATATAGTCAGCCACCTTGGTACGAACATAATCTTTCTCAAGTGCAA	8	0.2	No Hit
CACCAACATTGTTGCACCTTGTCACCATGTCTCTGAATTCATCTTCATTTC	8	0.2	No Hit
GTTGGTATCTTTCCCACCAAGGTCTTGATGGGTTATGAACTACAACGTTTT	8	0.2	No Hit
CTTCATTTCCAGACCTTGTGCAGATTTTATAGCTGATTGGTTGGTATCTTT	8	0.2	No Hit
CCATACTTGAATTCTGTCACACGGCCATTTCCAAAGTACTCACTACCTTTA	7	0.17500000000000002	No Hit
GCTTGTTCCTGCAGGATTGCCTGCGCCACACATGTGGTTAATGACAGCATC	7	0.17500000000000002	No Hit
CCTGTTATTTGGATTGAGGTAACTTCCACAGGTACTGCTTGTTCCTGCAGG	7	0.17500000000000002	No Hit
CCAACCTTCTCCCCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTT	7	0.17500000000000002	No Hit
GTCTGCATTAATGGTCACTTCTTTTGTTACTCCATTGTTATTGGGTGGTCC	7	0.17500000000000002	No Hit
GTTTTCATTGGGTGGAGAGACCTGCACCCCTCCAAATCCCTTAGGAGCTAA	7	0.17500000000000002	No Hit
AGCATGGATTGCAATAAATGGGTCCTCAGCAGAGTTACTAATGGAAAAGTG	7	0.17500000000000002	No Hit
GCCTCACCACCCAGATCAATGACCTCTTGGAAAATGAAAGGTCTGCTTCCT	7	0.17500000000000002	No Hit
CTTGTGAATCCATAAGGATGAGCCAACATAAATCCGACAGCCATTTTATAC	7	0.17500000000000002	No Hit
CCAACATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTTATCTCCAGAG	7	0.17500000000000002	No Hit
GCCAACATAAATCCGACAGCCATTTTATACATTCTAGCATCCCAGAATGTC	7	0.17500000000000002	No Hit
ACTACCTTTAATTGCCTCACCACCCAGATCAATGACCTCTTGGAAAATGAA	7	0.17500000000000002	No Hit
GTTCATATAGTCAGCCACCTTGGTACGAACATAATCTTTCTCAAGTGCAAG	7	0.17500000000000002	No Hit
ATCACAGTATGTGCCAGCAGGAAGACCAGTCTGTAAAGTGGCTGACAAAGC	7	0.17500000000000002	No Hit
ATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTTATCTCCAGAGATGAC	7	0.17500000000000002	No Hit
CCAGAGATGACATCACAGTATGTGCCAGCAGGAAGACCAGTCTGTAAAGTG	6	0.15	No Hit
GTTCTGAATCCAGTCCACGTAGTTGCAGACCTTGGTGTATACACCAGGAGC	6	0.15	No Hit
CCCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTTGCGGATAACTG	6	0.15	No Hit
GCTGTTATTATCCCACCAGTTTGAAAAAGGCTGACCATTGACCACATTCCT	6	0.15	No Hit
CTCGCCATTCCACTTGCGGATAACTGTGCCAAGTTTTGCACCATACTTGAA	6	0.15	No Hit
GTCACACGGCCATTTCCAAAGTACTCACTACCTTTAATTGCCTCACCACCC	6	0.15	No Hit
CTCACTACCTTTAATTGCCTCACCACCCAGATCAATGACCTCTTGGAAAAT	6	0.15	No Hit
GACCTGATAAGCATCATTGTAGTTATCAATTTCTCCATTACATTTATTATC	6	0.15	No Hit
GCCATCGACCTTATCTCCAGAGATGACATCACAGTATGTGCCAGCAGGAAG	6	0.15	No Hit
GGTTGCTGTTATTATCCCACCAGTTTGAAAAAGGCTGACCATTGACCACAT	6	0.15	No Hit
GTACGAACATAATCTTTCTCAAGTGCAAGATCCAGAAGGCCAGTCAGACGA	6	0.15	No Hit
CGGTAACTTGACATTACTCTTGTGAATCCATAAGGATGAGCCAACATAAAT	6	0.15	No Hit
CTCGCTGATTATCATGGTTGTCCACAAACACAAGGGCTCTGTCAGAAGGCA	6	0.15	No Hit
GTCTGATAGTTGCTGATGCAATGGGCAGCTGTCAGAACCCAGTTGTTGGCC	6	0.15	No Hit
GGACATCTTCTCGCCATTCCACTTGCGGATAACTGTGCCAAGTTTTGCACC	5	0.125	No Hit
CCACATTCCTGAAGGCAACCATGTTCCTGATTTGACGCCATCTGTGTTCAC	5	0.125	No Hit
GTTATTTGGATTGAGGTAACTTCCACAGGTACTGCTTGTTCCTGCAGGATT	5	0.125	No Hit
GTCATTGCCACAAGTAGTGTCTGCATTAATGGTCACTTCTTTTGTTACTCC	5	0.125	No Hit
GTCCACAAACACAAGGGCTCTGTCAGAAGGCACCAAACCCCAACCTTCTCC	5	0.125	No Hit
GTGCCAGCAGGAAGACCAGTCTGTAAAGTGGCTGACAAAGCCCAGTCATCA	5	0.125	No Hit
CTCCACATCAACGACATCTTCTGCTTTAACATGAAAGTCAACTGTAGTAAG	5	0.125	No Hit
CTTGTCACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCAGATT	5	0.125	No Hit
ATTACATTTATTATCGTTAAAGTCCCAAGCAGAGTATGGAACTGCTGGGAA	5	0.125	No Hit
GTCACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCAGATTTTA	5	0.125	No Hit
ATCCTCCAGCACCATGTCCTCGCTGATTATCATGGTTGTCCACAAACACAA	5	0.125	No Hit
AGGAAATGTAAGCAGAGACTCTGGTGAAGACGGTGGGCTTCCTGGCGACAT	5	0.125	No Hit
CCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTTGCGGATAACTGT	5	0.125	No Hit
GCCAACATAAATCCAACAGCCATTTTATACATTCTAGCATCCCAGAATGTC	5	0.125	No Hit
GGCCACATGTGCTTAGCAGCATCAAGTCTGAACCCTGCTACTCCAATGTCA	5	0.125	No Hit
GCCAGTTCTGTCCTGCAGGGACACCTGCCAGGGCCAGGAGCCAGGGATAGC	5	0.125	No Hit
GTCTGGGTTGTTCACACCATTGCTGAGGGTGTTGCCCCAGCCAGAGATGAG	5	0.125	No Hit
GGGGTCTTGAGGGCATTGTATTTGGTCTTGCCCCAGCCAGTGGTGGCACAC	5	0.125	No Hit
GGTCTGCTTCCTTGGGAGAACCATTTTGTATTGAGATTATGCAGTTTGTCC	5	0.125	No Hit
GCTTCCTTGGGAGAACCATTTTGTATTGAGATTATGCAGTTTGTCCAAAAC	5	0.125	No Hit
GCTCACATTCCTTGGCAATATCAACCCAGCGCCACTCGAACAGGTGGACAA	5	0.125	No Hit
GTTGCTGTTATTATCCCACCAGTTTGAAAAAGGCTGACCATTGACCACATT	5	0.125	No Hit
GTTCCTGCAGGATTGCCTGCGCCACACATGTGGTTAATGACAGCATCCACA	5	0.125	No Hit
CCCTGTTATTTGGATTGAGGTAACTTCCACAGGTACTGCTTGTTCCTGCAG	5	0.125	No Hit
GGGCTCTGTCAGAAGGCACCAAACCCCAACCTTCTCCCCAGTTCTTTAAAT	5	0.125	No Hit
GCACCATACTTGAATTCTGTCACACGGCCATTTCCAAAGTACTCACTACCT	5	0.125	No Hit
CTTGGGAGAACCATTTTGTATTGAGATTATGCAGTTTGTCCAAAACTGCCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24820864 read2 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24820864_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.97	34.0	34.0	34.0	34.0	34.0
2	33.1685	34.0	34.0	34.0	34.0	34.0
3	32.0805	34.0	34.0	34.0	26.0	34.0
4	32.7555	34.0	34.0	34.0	34.0	34.0
5	32.699	34.0	34.0	34.0	34.0	34.0
6	32.644	34.0	34.0	34.0	34.0	34.0
7	32.6555	34.0	34.0	34.0	34.0	34.0
8	32.7625	34.0	34.0	34.0	34.0	34.0
9	32.7665	34.0	34.0	34.0	34.0	34.0
10	32.7405	34.0	34.0	34.0	34.0	34.0
11	32.6105	34.0	34.0	34.0	34.0	34.0
12	32.5365	34.0	34.0	34.0	26.0	34.0
13	32.7795	34.0	34.0	34.0	34.0	34.0
14	32.641	34.0	34.0	34.0	34.0	34.0
15	32.6375	34.0	34.0	34.0	34.0	34.0
16	32.534	34.0	34.0	34.0	34.0	34.0
17	32.668	34.0	34.0	34.0	34.0	34.0
18	32.589	34.0	34.0	34.0	34.0	34.0
19	32.5875	34.0	34.0	34.0	34.0	34.0
20	32.6335	34.0	34.0	34.0	34.0	34.0
21	32.5355	34.0	34.0	34.0	34.0	34.0
22	32.547	34.0	34.0	34.0	34.0	34.0
23	32.5	34.0	34.0	34.0	34.0	34.0
24	32.537	34.0	34.0	34.0	34.0	34.0
25	32.4165	34.0	34.0	34.0	34.0	34.0
26	32.6385	34.0	34.0	34.0	34.0	34.0
27	32.5595	34.0	34.0	34.0	34.0	34.0
28	32.669	34.0	34.0	34.0	34.0	34.0
29	32.4885	34.0	34.0	34.0	34.0	34.0
30	32.4965	34.0	34.0	34.0	34.0	34.0
31	32.3335	34.0	34.0	34.0	26.0	34.0
32	32.427	34.0	34.0	34.0	34.0	34.0
33	32.343	34.0	34.0	34.0	26.0	34.0
34	32.3465	34.0	34.0	34.0	26.0	34.0
35	32.397	34.0	34.0	34.0	34.0	34.0
36	32.2105	34.0	34.0	34.0	26.0	34.0
37	32.392	34.0	34.0	34.0	34.0	34.0
38	32.164	34.0	34.0	34.0	26.0	34.0
39	32.2085	34.0	34.0	34.0	26.0	34.0
40	32.454	34.0	34.0	34.0	34.0	34.0
41	32.2325	34.0	34.0	34.0	26.0	34.0
42	32.116	34.0	34.0	34.0	26.0	34.0
43	32.14	34.0	34.0	34.0	26.0	34.0
44	32.2595	34.0	34.0	34.0	26.0	34.0
45	32.1755	34.0	34.0	34.0	26.0	34.0
46	32.2485	34.0	34.0	34.0	26.0	34.0
47	32.439	34.0	34.0	34.0	34.0	34.0
48	32.1635	34.0	34.0	34.0	26.0	34.0
49	32.127	34.0	34.0	34.0	26.0	34.0
50	32.226	34.0	34.0	34.0	26.0	34.0
51	31.9545	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	13.0
17	14.0
18	18.0
19	20.0
20	21.0
21	21.0
22	16.0
23	15.0
24	25.0
25	15.0
26	36.0
27	37.0
28	54.0
29	62.0
30	98.0
31	154.0
32	582.0
33	2205.0
34	581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2	25.6	4.375	26.825
2	28.525	24.375	33.425	13.675
3	21.925	25.35	30.349999999999998	22.375
4	25.45	32.375	21.625	20.549999999999997
5	30.9	32.95	18.9	17.25
6	24.775	35.05	18.725	21.45
7	22.075	22.25	34.475	21.2
8	22.85	20.7	27.925	28.525
9	22.45	22.525000000000002	30.175	24.85
10	27.025	30.375000000000004	22.95	19.650000000000002
11	30.575000000000003	25.900000000000002	19.55	23.974999999999998
12	27.025	23.275000000000002	24.85	24.85
13	26.025	23.0	27.800000000000004	23.175
14	26.85	26.950000000000003	24.725	21.475
15	26.775	26.400000000000002	24.075	22.75
16	28.599999999999998	22.05	26.05	23.3
17	27.85	24.325	25.775	22.05
18	25.55	24.875	25.650000000000002	23.925
19	27.175	25.35	23.45	24.025
20	27.575	27.825	22.0	22.6
21	24.825	26.55	24.349999999999998	24.275
22	26.1	26.3	24.075	23.525
23	27.6	26.8	22.25	23.35
24	26.424999999999997	24.375	26.474999999999998	22.725
25	26.650000000000002	24.95	25.3	23.1
26	28.075	24.75	24.775	22.400000000000002
27	25.650000000000002	24.775	26.450000000000003	23.125
28	27.6	25.5	24.349999999999998	22.55
29	28.875	25.674999999999997	22.575	22.875
30	26.650000000000002	26.1	24.325	22.925
31	25.174999999999997	23.849999999999998	26.625	24.349999999999998
32	28.299999999999997	25.650000000000002	23.7	22.35
33	26.900000000000002	26.0	25.074999999999996	22.025
34	27.474999999999998	25.75	24.375	22.400000000000002
35	26.700000000000003	25.724999999999998	24.474999999999998	23.1
36	26.700000000000003	25.7	25.0	22.6
37	26.025	25.724999999999998	25.624999999999996	22.625
38	28.125	25.7	23.599999999999998	22.575
39	26.275	26.85	22.925	23.95
40	25.6	25.75	25.650000000000002	23.0
41	26.5	28.549999999999997	22.45	22.5
42	24.8	27.825	23.825	23.549999999999997
43	25.7	26.875	25.074999999999996	22.35
44	26.224999999999998	27.575	24.375	21.825
45	24.675	28.175	23.1	24.05
46	24.5	28.549999999999997	23.95	23.0
47	26.1	28.025	23.65	22.225
48	24.0	28.299999999999997	23.9	23.799999999999997
49	24.275	28.1	23.775	23.849999999999998
50	23.925	28.199999999999996	24.55	23.325000000000003
51	25.525	27.3	23.525	23.65
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	2.0
10	1.0
11	1.5
12	2.0
13	2.0
14	2.0
15	2.5
16	3.0
17	2.5
18	2.0
19	2.5
20	3.0
21	4.0
22	5.0
23	4.0
24	3.0
25	8.0
26	12.5
27	12.0
28	15.0
29	18.0
30	19.5
31	21.0
32	26.5
33	32.0
34	65.5
35	99.0
36	139.0
37	179.0
38	208.5
39	238.0
40	256.5
41	275.0
42	295.5
43	316.0
44	307.5
45	299.0
46	316.5
47	334.0
48	352.0
49	370.0
50	344.5
51	319.0
52	307.5
53	296.0
54	284.0
55	272.0
56	245.5
57	219.0
58	212.5
59	206.0
60	166.5
61	127.0
62	120.0
63	113.0
64	94.5
65	76.0
66	60.0
67	44.0
68	30.0
69	16.0
70	13.0
71	10.0
72	9.0
73	8.0
74	5.5
75	2.5
76	2.0
77	3.5
78	5.0
79	5.0
80	5.0
81	4.0
82	3.0
83	2.5
84	2.0
85	1.0
86	0.0
87	1.5
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	3.5
94	6.0
95	4.5
96	3.0
97	2.5
98	2.0
99	14.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3041018387553	56.775
2	11.421499292786422	16.150000000000002
3	3.536067892503536	7.5
4	1.768033946251768	5.0
5	1.3437057991513437	4.75
6	0.7779349363507779	3.3000000000000003
7	0.1768033946251768	0.8750000000000001
8	0.07072135785007072	0.4
9	0.31824611032531824	2.025
>10	0.2828854314002829	3.225
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCC	26	0.65	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	25	0.625	No Hit
AGAGGATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTTA	16	0.4	No Hit
GTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAGATCAGAATGA	14	0.35000000000000003	No Hit
AGCAAAATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCT	14	0.35000000000000003	No Hit
GTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTCTTTAACAATGATGAC	12	0.3	No Hit
GTTGTAGTTCATAACCCATCAAGACCTTGGTGGGAAAGATACCAACCAATC	11	0.27499999999999997	No Hit
GGTCTCTCCACCCAATGAAAACGTTGTAGTTCATAACCCATCAAGACCTTG	11	0.27499999999999997	No Hit
AGCAGGGTTCAGACTTGATGCTGCTAAGCACATGTGGCCTGGAGACATAAA	9	0.22499999999999998	No Hit
AAAAGATCAGAATGACTGGATTGGACCACCCAATAACAATGGAGTAACAAA	9	0.22499999999999998	No Hit
CAGAATTCAAGTATGGTGCAAAACTTGGCACAGTTATCCGCAAGTGGAATG	9	0.22499999999999998	No Hit
GTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGACATTGGAGTA	9	0.22499999999999998	No Hit
CTCAATACAAAATGGTTCTCCCAAGGAAGCAGACCTTTCATTTTCCAAGAG	9	0.22499999999999998	No Hit
AGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACC	9	0.22499999999999998	No Hit
GATAATAACAGCAACCAAGTAGCTTTTAGCAGAGGAAACAGAGGATTCATT	9	0.22499999999999998	No Hit
GTCAAATCAGGAACATGGTTGCCTTCAGGAATGTGGTCAATGGTCAGCCTT	9	0.22499999999999998	No Hit
AATAACAGCAACCAAGTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTC	9	0.22499999999999998	No Hit
CTGGGCTTTGTCAGCCACTTTACAGACTGGTCTTCCTGCTGGCACATACTG	8	0.2	No Hit
AATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATA	8	0.2	No Hit
CGTCAAATCAGGAACATGGTTGCCTTCAGGAATGTGGTCAATGGTCAGCCT	7	0.17500000000000002	No Hit
GGGAATTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAAT	7	0.17500000000000002	No Hit
GTTCAGACTTGATGCTGCTAAGCACATGTGGCCTGGAGACATAAAGGCAGT	7	0.17500000000000002	No Hit
GTTACCTCAATCCAAATAACAGGGAATTCCCAGCAGTTCCATACTCTGCTT	7	0.17500000000000002	No Hit
GTCAGCCTTTTTCAAACTGGTGGGATAATAACAGCAACCAAGTAGCTTTTA	7	0.17500000000000002	No Hit
ATATGAACCATCTCATTGACATTGGAGTAGCAGGGTTCAGACTTGATGCTG	6	0.15	No Hit
CATTGATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGG	6	0.15	No Hit
GAAAGATTATGTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGA	6	0.15	No Hit
AGCAGACCTTTCATTTTCCAAGAGGTCATTGATCTGGGTGGTGAGGCAATT	6	0.15	No Hit
GTGGAAGTTACCTCAATCCAAATAACAGGGAATTCCCAGCAGTTCCATACT	6	0.15	No Hit
AGATTATGTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGACAT	6	0.15	No Hit
GTCGGATTTATGTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGT	6	0.15	No Hit
AGAATGTATAAAATGGCTGTCGGATTTATGTTGGCTCATCCTTATGGATTC	6	0.15	No Hit
GTAATGTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAGATCAG	6	0.15	No Hit
CCATGATCAGGACACTGCTGCTATCTGCCTTGGTGGCTGGAGCCCTCAGCT	6	0.15	No Hit
GATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTTACAGA	6	0.15	No Hit
GTCCTATTTAAAGAACTGGGGAGAAGGTTGGGGTTTGGTGCCTTCTGACAG	6	0.15	No Hit
CTTGTGTTTGTGGACAACCATGATAATCAGCGAGGACATGGTGCTGGAGGA	6	0.15	No Hit
CAGACCTTTCATTTTCCAAGAGGTCATTGATCTGGGTGGTGAGGCAATTAA	6	0.15	No Hit
ATCAGAATGACTGGATTGGACCACCCAATAACAATGGAGTAACAAAAGAAG	6	0.15	No Hit
GGAAAAGATCAGAATGACTGGATTGGACCACCCAATAACAATGGAGTAACA	6	0.15	No Hit
CAGAGGATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTT	6	0.15	No Hit
GTCCTATTTAAAGAACTGGGGAGAAGGTTGGGGTATGGTGCCTTCTGACAG	6	0.15	No Hit
GTGGCCTGGAGACATAAAGGCAGTTTTGGACAAACTGCATAATCTCAATAC	6	0.15	No Hit
GTCATTAACCACATGTGTGGCGCAGGCAATCCTGCAGGAACAAGCAGTACC	6	0.15	No Hit
CTTTAACGATAATAAATGTAATGGAGAAATTGATAACTACAATGATGCTTA	6	0.15	No Hit
GGTCAGCCTTTTTCAAACTGGTGGGATAATAACAGCAACCAAGTAGCTTTT	6	0.15	No Hit
AAATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAAT	5	0.125	No Hit
GTCAGAAATTGTCGTCTGACTGGCCTTCTGGATCTTGCACTTGAGAAAGAT	5	0.125	No Hit
CATCCATCCTGACATTCTGGGATGCTAGAATGTATAAAATGGCTGTTGGAT	5	0.125	No Hit
GGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGTGTGACAGAATTCAA	5	0.125	No Hit
ATTGGGTTCTGCTGGGCTCAATATGACCCACATACTTCAGATGGGAGGACT	5	0.125	No Hit
GTTCGAGTGGCGCTGGGTTGATATTGCCAAGGAATGTGAGCGATACTTAGC	5	0.125	No Hit
CATTGACATTGGAGTAGCAGGGTTCAGACTTGATGCTGCTAAGCACATGTG	5	0.125	No Hit
GAAAGATACCAACCAATCAGCTATAAAATCTGCACAAGGTCTGGAAATGAA	5	0.125	No Hit
GGATAATAACAGCAACCAAGTAGCTTTTAGCAGAGGAAACAGAGGATTCAT	5	0.125	No Hit
CAATAACAATGGAGTAACAAAAGAAGTGACCATTAATGCAGACACTACTTG	5	0.125	No Hit
GTATAAAATGGCTGTCGGATTTATGTTGGCTCATCCTTATGGATTCACAAG	5	0.125	No Hit
GCAAAATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTC	5	0.125	No Hit
CTTAGCTCCTAAGGGATTTGGAGGGGTGCAGGTCTCTCCACCCAATGAAAA	5	0.125	No Hit
CAATGGAGTAACAAAAGAAGTGACCATTAATGCAGACACTACTTGTGGCAA	5	0.125	No Hit
GTCAAATCAGGAACATGGTTGCCTTCAGAAATGTGGTCAATGGTCAGCCTT	5	0.125	No Hit
ATATGACCCACATACTTCAGATGGGAGGACTGCTATTGTCCACCTGTTCGA	5	0.125	No Hit
CATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACACAGATG	5	0.125	No Hit
GTGACCATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACAC	5	0.125	No Hit
GTTGGATTTATGTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGT	5	0.125	No Hit
GTGAACACAGATGGCGTCAAATCAGGAACATGGTTGCCTTCAGGAATGTGG	5	0.125	No Hit
GTGAACACAGATGGCGTCAAATCAGGAACATGGTTGCCTTCAGAAATGTGG	5	0.125	No Hit
GGTGCAACAATGTTGGTGTCCGTATTTATGTGGATGCTGTCATTAACCACA	5	0.125	No Hit
CTGGGGAGAAGGTTGGGGTATGGTGCCTTCTGACAGAGCCCTTGTGTTTGT	5	0.125	No Hit
AGTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTCTTTAACAATGATGA	5	0.125	No Hit
CCTGCTTTGCCCTTGTGGGCGCCACCTTTGGCTGTGGGGTCCCTGCCATCC	5	0.125	No Hit
ATAAAATCTGCACAAGGTCTGGAAATGAAGATGAATTCAGAGACATGGTGA	5	0.125	No Hit
AGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGTGTGACAGAATTCA	5	0.125	No Hit
ATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTTACAGAC	5	0.125	No Hit
GTGGATGCTGTCATTAACCACATGTGTGGCGCAGGCAATCCTGCAGGAACA	5	0.125	No Hit
CCCACATACTTCAGATGGGAGGACTGCTATTGTCCACCTGTTCGAGTGGCG	5	0.125	No Hit
CAATGACTGGGTCTGTGAACACAGATGGCGTCAAATCAGGAACATGGTTGC	5	0.125	No Hit
CATAAAGGCAGTTTTGGACAAACTGCATAATCTCAATACAAAATGGTTCTC	5	0.125	No Hit
ATTATGTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGACATTG	5	0.125	No Hit
GGACAACCATGATAATCAGCGAGGACATGGTGCTGGAGGATCATCCATCCT	5	0.125	No Hit
CTACAATGATGCTTATCAGGTCAGAAATTGTCGTCTGACTGGCCTTCTGGA	5	0.125	No Hit
GGCAGTTTTGGACAAACTGCATAATCTCAATACAAAATGGTTCTCCCAAGG	5	0.125	No Hit
GGACAAACTGCATAATCTCAATACAAAATGGTTCTCCCAAGGAAGCAGACC	5	0.125	No Hit
GTATAAAATGGCTGTTGGATTTATGTTGGCTCATCCTTATGGATTCACAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
Read 1491590 spots for SRR24820864.sra
Written 1491590 spots for SRR24820864.sra
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
Read 1491585 spots for SRR24820864.sra
Written 1491585 spots for SRR24820864.sra
SRR ids: ['SRR24820864.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_c2e1m8y0
SRR24820864.sra spots: 11932685
blocks: [[1, 1491585], [1491586, 2983170], [2983171, 4474755], [4474756, 5966340], [5966341, 7457925], [7457926, 8949510], [8949511, 10441095], [10441096, 11932685]]
SRR24820864 file size 2049788
SRR24820864 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24820864 SRR24820864_1.fastq SRR24820864_2.fastq
Input file:	SRR24820864_1.fastq
Paired file:	SRR24820864_2.fastq
trimmed:	SRR24820864-trimmed-pair1.fastq, SRR24820864-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun Jul  5 08:37:39 2026 >> started

Sun Jul  5 08:37:55 2026 >> done (16.260s)
11932685 read pairs processed; of these:
     563 ( 0.00%) short read pairs filtered out after trimming by size control
    3563 ( 0.03%) empty read pairs filtered out after trimming by size control
11928559 (99.97%) read pairs available; of these:
    6355 ( 0.05%) trimmed read pairs available after processing
11922204 (99.95%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      18	  0.00%
 19	       9	  0.00%
 20	      44	  0.00%
 21	      13	  0.00%
 22	      17	  0.00%
 23	      19	  0.00%
 24	      27	  0.00%
 25	      70	  0.00%
 26	      22	  0.00%
 27	      25	  0.00%
 28	      26	  0.00%
 29	      22	  0.00%
 30	      21	  0.00%
 31	      26	  0.00%
 32	      30	  0.00%
 33	      46	  0.00%
 34	      49	  0.00%
 35	      66	  0.00%
 36	      80	  0.00%
 37	      87	  0.00%
 38	     131	  0.00%
 39	     159	  0.00%
 40	     191	  0.00%
 41	     201	  0.00%
 42	     284	  0.00%
 43	     329	  0.00%
 44	     350	  0.00%
 45	     389	  0.00%
 46	     479	  0.00%
 47	     553	  0.00%
 48	     632	  0.01%
 49	     827	  0.01%
 50	    1113	  0.01%
 51	11922204	 99.95%
11928559 reads passed initial QC


criterion=sequence-density
sequence-density=1.00
sequence-density-rank=1
fanout-score=3.77
fanout-score-rank=18
prefix-density=1.33
prefix-fanout=2.8
sequence=TTGCCCCAGCCAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=31.97
fanout-score-rank=1
prefix-density=0.16
prefix-fanout=1.0
sequence=ATAATGATAGGATTTTATTAAGATGTTTTCAGGGGTCCAATTGGTCATTACATCAAGAAAATTATGAGAAAGATTAGAATCTGCAAACCTCCAAACAAACGAAGTCACATCAGACAAGGAATGAACAGTAAGAATTTTAAAATAAGGCAATCCAGATTCTTCACTAATATGGATGCTCTAGGACATAATTGGCAATGTACTTGACTGCAAGCATTGTCTCCTCACAGGTTTGGCGGATCTGAGACTCAGGAAGCAGAAAGCCAAAGAAGCCTTTATCCCGGAGTTCAAAGGTAAAGGAATATTTGATTCCCTGATCATAAGCCCAGTCGTCAGATCCCCCAGCAGCAGGATAGATCGTTGTAGCTCCTGGGCCATATGTGTACTTGGTGCCATGCAGAGTGGAAAGCTCCTTTGCTGCACCTTTCACCAGGGCATTCAGCTCCTCATAGTTCTCGGGCAGCTTATAGTCGTAGGAGTAAGGATAGAGCATCATCTGTGAGTAGGAGTGGAC


criterion=sequence-density
sequence-density=0.51
sequence-density-rank=1
fanout-score=2.78
fanout-score-rank=17
prefix-density=1.42
prefix-fanout=1.0
sequence=GTTGCCTTCAGAAATGTGGTCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=30
fanout-score=16.69
fanout-score-rank=1
prefix-density=0.15
prefix-fanout=1.0
sequence=CAATGAATTCACTCCTGTTCCTGGCCCTTGTGGG
SRR24820864 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 05 08:38:12
                             Started mapping on |	Jul 05 08:38:13
                                    Finished on |	Jul 05 08:40:04
       Mapping speed, Million of reads per hour |	386.87

                          Number of input reads |	11928559
                      Average input read length |	101
                                    UNIQUE READS:
                   Uniquely mapped reads number |	6124063
                        Uniquely mapped reads % |	51.34%
                          Average mapped length |	101.60
                       Number of splices: Total |	3303049
            Number of splices: Annotated (sjdb) |	3296900
                       Number of splices: GT/AG |	3296218
                       Number of splices: GC/AG |	4756
                       Number of splices: AT/AC |	304
               Number of splices: Non-canonical |	1771
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.71
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.35
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3509640
             % of reads mapped to multiple loci |	29.42%
        Number of reads mapped to too many loci |	1425544
             % of reads mapped to too many loci |	11.95%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.27%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2294856	2294856	2294856
N_multimapping	3509640	3509640	3509640
N_noFeature	183441	6039431	198374
N_ambiguous	188024	5386	153774
UnstrandedReadsAssigned:5752598 PositiveStrandReadsAssigned:79246 NegativeStrandReadsAssigned:5771915
Dataset is classified negative stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR24820864 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24820864-trimmed-pair1.fastq
                             SRR24820864-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 11,928,559 reads, 10,999,559 reads pseudoaligned
[quant] estimated average fragment length: 157.805
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 909 rounds

 109283 SRR24820864.ke.tsv
  52636 SRR24820864.se.tsv
 161919 total
==> SRR24820864.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7.75	0	0
ENSMUST00000196221.1	9	3	0	0
ENSMUST00000179664.1	11	5	0	0
ENSMUST00000178537.1	12	6	0	0
ENSMUST00000178862.1	14	8	0	0
ENSMUST00000179520.1	11	5	0	0
ENSMUST00000179883.1	16	7.75	0	0
ENSMUST00000195858.1	10	4	0	0
ENSMUST00000179932.1	12	6	0	0

==> SRR24820864.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24820864 completed mapping pipeline successfully
