Starting /dee2/code/volunteer_pipeline.sh SRR24820675
    current disk space = 1415996920
    free memory = 191605972 
SRR24820675 SRAfilesize
642071f3ec5a94f94e30ab9dfc063779  SRR24820675.sra
SRR24820675.sra file validated
SRR24820675 is paired end
SRR24820675 is conventional basespace
SRR24820675 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24820675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6725	34.0	34.0	34.0	34.0	34.0
2	32.5245	34.0	34.0	34.0	34.0	34.0
3	32.3305	34.0	34.0	34.0	26.0	34.0
4	32.906	34.0	34.0	34.0	34.0	34.0
5	33.001	34.0	34.0	34.0	34.0	34.0
6	32.9695	34.0	34.0	34.0	34.0	34.0
7	32.919	34.0	34.0	34.0	34.0	34.0
8	32.941	34.0	34.0	34.0	34.0	34.0
9	32.8235	34.0	34.0	34.0	34.0	34.0
10	33.0505	34.0	34.0	34.0	34.0	34.0
11	33.0065	34.0	34.0	34.0	34.0	34.0
12	32.948	34.0	34.0	34.0	34.0	34.0
13	32.914	34.0	34.0	34.0	34.0	34.0
14	32.9335	34.0	34.0	34.0	34.0	34.0
15	33.008	34.0	34.0	34.0	34.0	34.0
16	33.07	34.0	34.0	34.0	34.0	34.0
17	33.048	34.0	34.0	34.0	34.0	34.0
18	33.0355	34.0	34.0	34.0	34.0	34.0
19	33.104	34.0	34.0	34.0	34.0	34.0
20	33.001	34.0	34.0	34.0	34.0	34.0
21	33.0315	34.0	34.0	34.0	34.0	34.0
22	32.873	34.0	34.0	34.0	34.0	34.0
23	32.9455	34.0	34.0	34.0	34.0	34.0
24	32.9915	34.0	34.0	34.0	34.0	34.0
25	32.981	34.0	34.0	34.0	34.0	34.0
26	33.029	34.0	34.0	34.0	34.0	34.0
27	32.904	34.0	34.0	34.0	34.0	34.0
28	32.9175	34.0	34.0	34.0	34.0	34.0
29	32.9015	34.0	34.0	34.0	34.0	34.0
30	32.8745	34.0	34.0	34.0	34.0	34.0
31	33.0105	34.0	34.0	34.0	34.0	34.0
32	32.8635	34.0	34.0	34.0	34.0	34.0
33	32.964	34.0	34.0	34.0	34.0	34.0
34	32.8955	34.0	34.0	34.0	34.0	34.0
35	32.9445	34.0	34.0	34.0	34.0	34.0
36	32.9135	34.0	34.0	34.0	34.0	34.0
37	32.8745	34.0	34.0	34.0	34.0	34.0
38	32.9305	34.0	34.0	34.0	34.0	34.0
39	32.8815	34.0	34.0	34.0	34.0	34.0
40	32.854	34.0	34.0	34.0	34.0	34.0
41	32.7855	34.0	34.0	34.0	34.0	34.0
42	32.9135	34.0	34.0	34.0	34.0	34.0
43	32.7325	34.0	34.0	34.0	34.0	34.0
44	32.7595	34.0	34.0	34.0	34.0	34.0
45	32.7805	34.0	34.0	34.0	34.0	34.0
46	32.781	34.0	34.0	34.0	34.0	34.0
47	32.7705	34.0	34.0	34.0	34.0	34.0
48	32.8605	34.0	34.0	34.0	34.0	34.0
49	32.8105	34.0	34.0	34.0	34.0	34.0
50	32.7145	34.0	34.0	34.0	34.0	34.0
51	32.627	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	5.0
23	8.0
24	21.0
25	23.0
26	51.0
27	62.0
28	83.0
29	109.0
30	114.0
31	190.0
32	399.0
33	1958.0
34	976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.975	11.05	2.45	39.525
2	18.75	10.65	42.699999999999996	27.900000000000002
3	17.625	12.6	26.150000000000002	43.625
4	22.925	24.65	22.55	29.875
5	28.65	28.675	23.150000000000002	19.525000000000002
6	23.575	27.400000000000002	24.0	25.025
7	16.45	28.849999999999998	39.225	15.475
8	20.525	21.55	35.3	22.625
9	20.424999999999997	19.275000000000002	36.525	23.775
10	22.5	33.375	25.1	19.025
11	26.55	27.925	23.150000000000002	22.375
12	23.9	23.075000000000003	28.9	24.125
13	24.7	24.2	27.750000000000004	23.35
14	26.25	25.35	25.674999999999997	22.725
15	23.125	23.5	26.525	26.85
16	24.425	26.575	23.400000000000002	25.6
17	23.75	26.85	25.1	24.3
18	22.575	24.375	28.299999999999997	24.75
19	25.85	24.75	25.6	23.799999999999997
20	25.0	25.724999999999998	26.375	22.900000000000002
21	23.474999999999998	25.75	28.499999999999996	22.275
22	24.425	24.525	27.425	23.625
23	23.150000000000002	23.674999999999997	28.275	24.9
24	22.525000000000002	23.974999999999998	28.975	24.525
25	23.799999999999997	24.65	28.249999999999996	23.3
26	24.55	24.425	27.0	24.025
27	21.825	25.525	25.8	26.85
28	24.65	26.55	26.424999999999997	22.375
29	25.4	26.900000000000002	26.025	21.675
30	24.175	23.474999999999998	26.150000000000002	26.200000000000003
31	22.475	25.624999999999996	25.724999999999998	26.174999999999997
32	25.074999999999996	27.900000000000002	24.224999999999998	22.8
33	24.542950162784873	22.789882294014525	26.195842724768344	26.471324818432258
34	24.575	25.374999999999996	25.275	24.775
35	24.0	22.75	28.9	24.349999999999998
36	23.325000000000003	25.074999999999996	27.450000000000003	24.15
37	25.4	26.174999999999997	24.025	24.4
38	23.7	23.5	27.3	25.5
39	19.475	25.474999999999998	27.725	27.325
40	22.475	26.3	25.224999999999998	26.0
41	22.35	25.575	26.75	25.324999999999996
42	22.025	23.45	26.700000000000003	27.825
43	25.35	25.924999999999997	23.5	25.224999999999998
44	23.525	26.1	26.275	24.099999999999998
45	24.45	26.05	25.374999999999996	24.125
46	25.025	27.625	23.974999999999998	23.375
47	25.025	25.775	26.125	23.075000000000003
48	22.6	25.025	25.5	26.875
49	22.875	27.575	23.775	25.775
50	21.575	27.675	26.700000000000003	24.05
51	23.549999999999997	25.45	25.825	25.174999999999997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.5
16	2.0
17	1.5
18	1.0
19	2.0
20	3.0
21	3.5
22	4.0
23	3.5
24	3.0
25	4.5
26	9.0
27	12.0
28	25.0
29	38.0
30	37.0
31	36.0
32	37.0
33	38.0
34	46.0
35	54.0
36	82.5
37	111.0
38	169.5
39	228.0
40	284.5
41	341.0
42	330.0
43	319.0
44	328.5
45	338.0
46	381.5
47	425.0
48	376.0
49	327.0
50	326.5
51	326.0
52	311.0
53	296.0
54	274.5
55	253.0
56	228.5
57	204.0
58	212.0
59	220.0
60	181.0
61	142.0
62	131.5
63	121.0
64	107.5
65	94.0
66	63.0
67	32.0
68	22.5
69	13.0
70	11.0
71	9.0
72	5.0
73	1.0
74	0.5
75	0.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.17500000000000002
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.75182481751825	56.00000000000001
2	10.072992700729927	13.8
3	2.846715328467153	5.8500000000000005
4	2.116788321167883	5.800000000000001
5	1.094890510948905	3.75
6	0.5474452554744526	2.25
7	0.25547445255474455	1.225
8	0.40145985401459855	2.1999999999999997
9	0.072992700729927	0.44999999999999996
>10	0.8394160583941607	8.674999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCAGCATGGATTGCAATAAATGGGTCCTCAGCAGAGTTACTAATGGAAAA	29	0.7250000000000001	No Hit
CTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAGTTTGAAAAAG	28	0.7000000000000001	No Hit
GTCCAGTGCAATTGCCATCGACCTTATCTCCAGAGATGACATCACAGTATG	23	0.575	No Hit
GCCACAAGTAGTGTCTGCATTAATGGTCACTTCTTTTGTTACTCCATTGTT	19	0.475	No Hit
GTCTGCATTAATGGTCACTTCTTTTGTTACTCCATTGTTATTGGGTGGTCC	19	0.475	No Hit
GTCCTCAGCAGAGTTACTAATGGAAAAGTGAGCTTTGCCATCACTGCCAAC	19	0.475	No Hit
CTTGGCAATATCAACCCAGCGCCACTCGAACAGGTGGACAATAGCAGTCCT	18	0.44999999999999996	No Hit
GTCCAATCCAGTCATTCTGATCTTTTCCATTCTGGAAATTTCTATTCCAAC	17	0.42500000000000004	No Hit
CCTGATTTGACGCCATCTGTGTTCACAGACCCAGTCATTGCCACAAGTAGT	16	0.4	No Hit
CTCTGTTTCCTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAGT	15	0.375	No Hit
CTCCATTACATTTATTATCGTTAAAGTCCCAAGCAGAGTATGGAACTGCTG	15	0.375	No Hit
GCCATTTCCAAAGTACTCACTACCTTTAATTGCCTCACCACCCAGATCAAT	14	0.35000000000000003	No Hit
GTCACTTCTTTTGTTACTCCATTGTTATTGGGTGGTCCAATCCAGTCATTC	12	0.3	No Hit
CTCCATTGTTATTGGGTGGTCCAATCCAGTCATTCTGATCTTTTCCATTCT	12	0.3	No Hit
CCAGTCATTGCCACAAGTAGTGTCTGCATTAATGGTCACTTCTTTTGTTAC	11	0.27499999999999997	No Hit
GTCTGCTTCCTTGGGAGAACCATTTTGTATTGAGATTATGCAGTTTGTCCA	10	0.25	No Hit
GCCACCTTGGTACGAACATAATCTTTCTCAAGTGCAAGATCCAGAAGGCCA	10	0.25	No Hit
GCCATCACTGCCAACATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTT	10	0.25	No Hit
GCCAACATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTTATCTCCAGA	10	0.25	No Hit
GTGCAATTGCCATCGACCTTATCTCCAGAGATGACATCACAGTATGTGCCA	10	0.25	No Hit
GTTGGTATCTTTCCCACCAAGGTCTTGATGGGTTATGAACTACAACGTTTT	10	0.25	No Hit
CCTCTGTTTCCTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAG	10	0.25	No Hit
GCCAGCAGGAAGACCAGTCTGTAAAGTGGCTGACAAAGCCCAGTCATCATT	10	0.25	No Hit
CCCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTTGCGGATAACTG	9	0.22499999999999998	No Hit
GCCATTTTATACATTCTAGCATCCCAGAATGTCAGGATGGATGATCCTCCA	9	0.22499999999999998	No Hit
CTCCAATGTCAATGAGATGGTTCATATAGTCAGCCACCTTGGTACGAACAT	8	0.2	No Hit
CTCCAGGCCACATGTGCTTAGCAGCATCAAGTCTGAACCCTGCTACTCCAA	8	0.2	No Hit
GCACCTTGTCACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCA	8	0.2	No Hit
CACCAGTTTGAAAAAGGCTGACCATTGACCACATTCCTGAAGGCAACCATG	8	0.2	No Hit
CTCGCCATTCCACTTGCGGATAACTGTGCCAAGTTTTGCACCATACTTGAA	8	0.2	No Hit
CGCCATTCCACTTGCGGATAACTGTGCCAAGTTTTGCACCATACTTGAATT	8	0.2	No Hit
GCCAACATAAATCCGACAGCCATTTTATACATTCTAGCATCCCAGAATGTC	8	0.2	No Hit
GTTCATATAGTCAGCCACCTTGGTACGAACATAATCTTTCTCAAGTGCAAG	8	0.2	No Hit
CCTCACCACCCAGATCAATGACCTCTTGGAAAATGAAAGGTCTGCTTCCTT	8	0.2	No Hit
GTTGCTGTTATTATCCCACCAGTTTGAAAAAGGCTGACCATTGACCACATT	8	0.2	No Hit
GGTTGCTGTTATTATCCCACCAGTTTGAAAAAGGCTGACCATTGACCACAT	8	0.2	No Hit
CTTGTCACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCAGATT	7	0.17500000000000002	No Hit
AGCATGGATTGCAATAAATGGGTCCTCAGCAGAGTTACTAATGGAAAAGTG	7	0.17500000000000002	No Hit
GCCTCACCACCCAGATCAATGACCTCTTGGAAAATGAAAGGTCTGCTTCCT	7	0.17500000000000002	No Hit
CCTCTGCTAAAAGCTACTTGGTTGCTGTTATTATCCCACCAGTTTGAAAAA	7	0.17500000000000002	No Hit
CCCCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTTGCGGATAACT	7	0.17500000000000002	No Hit
GTACGAACATAATCTTTCTCAAGTGCAAGATCCAGAAGGCCAGTCAGACGA	7	0.17500000000000002	No Hit
CTCGCTGATTATCATGGTTGTCCACAAACACAAGGGCTCTGTCAGAAGGCA	7	0.17500000000000002	No Hit
CCCCAACCTTCTCCCCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCAC	6	0.15	No Hit
CTGTTATTTGGATTGAGGTAACTTCCACAGGTACTGCTTGTTCCTGCAGGA	6	0.15	No Hit
CTCCAGCACCATGTCCTCGCTGATTATCATGGTTGTCCACAAACACAAGGG	6	0.15	No Hit
CCTGCTACTCCAATGTCAATGAGATGGTTCATATAGTCAGCCACCTTGGTA	6	0.15	No Hit
GTCACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCAGATTTTA	6	0.15	No Hit
CCCAGTCATTGCCACAAGTAGTGTCTGCATTAATGGTCACTTCTTTTGTTA	6	0.15	No Hit
GGTTCATATAGTCAGCCACCTTGGTACGAACATAATCTTTCTCAAGTGCAA	6	0.15	No Hit
CAGCCATTTTATACATTCTAGCATCCCAGAATGTCAGGATGGATGATCCTC	6	0.15	No Hit
GTCTTGATGGGTTATGAACTACAACGTTTTCATTGGGTGGAGAGACCTGCA	6	0.15	No Hit
GTTCAGGGTCCTGCTATTGAAGTTGGGATGCTTGATGATCTTGGCAGAATT	6	0.15	No Hit
CTCAGCTCTAACTCCTTCTTGCTTACTTCAGGTCTCATTGCAAAGCTAAGA	6	0.15	No Hit
AGTGCAATTGCCATCGACCTTATCTCCAGAGATGACATCACAGTATGTGCC	6	0.15	No Hit
GTCATTCTGATCTTTTCCATTCTGGAAATTTCTATTCCAACGGTAACTTGA	6	0.15	No Hit
CCTCAGCAGAGTTACTAATGGAAAAGTGAGCTTTGCCATCACTGCCAACAT	6	0.15	No Hit
GTTATGAACTACAACGTTTTCATTGGGTGGAGAGACCTGCACCCCTCCAAA	6	0.15	No Hit
GGACATCTTCTCGCCATTCCACTTGCGGATAACTGTGCCAAGTTTTGCACC	5	0.125	No Hit
GTGCCAAGTTTTGCACCATACTTGAATTCTGTCACACGGCCATTTCCAAAG	5	0.125	No Hit
CCAGAGATGACATCACAGTATGTGCCAGCAGGAAGACCAGTCTGTAAAGTG	5	0.125	No Hit
CACCATGTCTCTGAATTCATCTTCATTTCCAGACCTTGTGCAGATTTTATA	5	0.125	No Hit
GGGTCCTCAGCAGAGTTACTAATGGAAAAGTGAGCTTTGCCATCACTGCCA	5	0.125	No Hit
CATTGTTATTGGGTGGTCCAATCCAGTCATTCTGATCTTTTCCATTCTGGA	5	0.125	No Hit
GCATGGATTGCAATAAATGGGTCCTCAGCAGAGTTACTAATGGAAAAGTGA	5	0.125	No Hit
GTCTGAAGGTGGGTCAGGGGCAACCTCTGAGTCATCCTCAGGGGGAGTGAG	5	0.125	No Hit
GTCTCCAGGCCACATGTGCTTAGCAGCATCAAGTCTGAACCCTGCTACTCC	5	0.125	No Hit
GCCAGTTTGATCAGCATGATGTCATTGTCCAGGGTCCATGAATTATAATTG	5	0.125	No Hit
CTTGTGAATCCATAAGGATGAGCCAACATAAATCCGACAGCCATTTTATAC	5	0.125	No Hit
CCAGTTCTTTAAATAGGACATCTTCTCGCCATTCCACTTGCGGATAACTGT	5	0.125	No Hit
CATCATTGTAGTTATCAATTTCTCCATTACATTTATTATCGTTAAAGTCCC	5	0.125	No Hit
CTTGGTACGAACATAATCTTTCTCAAGTGCAAGATCCAGAAGGCCAGTCAG	5	0.125	No Hit
CACCAACATTGTTGCACCTTGTCACCATGTCTCTGAATTCATCTTCATTTC	5	0.125	No Hit
CTTAGGAGCTAAGTATCGCTCACATTCCTTGGCAATATCAACCCAGCGCCA	5	0.125	No Hit
CTTTAATTGCCTCACCACCCAGATCAATGACCTCTTGGAAAATGAAAGGTC	5	0.125	No Hit
GTCATGTTGGCAGCATCTGCTCTTACACTGCATACTGTTCAAACAGATCTC	5	0.125	No Hit
ATCACTGCCAACATTCACTCTAAGTCCAGTGCAATTGCCATCGACCTTATC	5	0.125	No Hit
CTTGTGAATCCATAAGGATGAGCCAACATAAATCCAACAGCCATTTTATAC	5	0.125	No Hit
GTCCAATTGGTCATTACATCAAGAAAATTATGAGAAAGATTAGAATCTGCA	5	0.125	No Hit
GACCTGATAAGCATCATTGTAGTTATCAATTTCTCCATTACATTTATTATC	5	0.125	No Hit
CTTGAATTCTGTCACACGGCCATTTCCAAAGTACTCACTACCTTTAATTGC	5	0.125	No Hit
ATCGTCAACAGTGGGCAGACACACGGCAGACACAGTCTCAGAGAACTGGGC	5	0.125	No Hit
CCCTGATCAAACTCTCCAGCTACCACCACATCGGTTGTCTTGACCCCGCAG	5	0.125	No Hit
CCAGTCTGTAAAGTGGCTGACAAAGCCCAGTCATCATTGTTAAAGACAATG	5	0.125	No Hit
CCCTGTTATTTGGATTGAGGTAACTTCCACAGGTACTGCTTGTTCCTGCAG	5	0.125	No Hit
GGGAGAACCATTTTGTATTGAGATTATGCAGTTTGTCCAAAACTGCCTTTA	5	0.125	No Hit
GTCAGGACTGTTCCCATTGGTCTGCAGCAGGCCCCAGCCTGTGACATAGCA	5	0.125	No Hit
CTCCAAATCCCTTAGGAGCTAAGTATCGCTCACATTCCTTGGCAATATCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24820675 read2 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24820675_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5555	34.0	34.0	34.0	34.0	34.0
2	32.941	34.0	34.0	34.0	34.0	34.0
3	32.6105	34.0	34.0	34.0	34.0	34.0
4	32.5765	34.0	34.0	34.0	34.0	34.0
5	32.599	34.0	34.0	34.0	34.0	34.0
6	32.5355	34.0	34.0	34.0	34.0	34.0
7	32.53	34.0	34.0	34.0	34.0	34.0
8	32.5365	34.0	34.0	34.0	34.0	34.0
9	32.588	34.0	34.0	34.0	34.0	34.0
10	32.61	34.0	34.0	34.0	34.0	34.0
11	32.594	34.0	34.0	34.0	34.0	34.0
12	32.3935	34.0	34.0	34.0	26.0	34.0
13	32.581	34.0	34.0	34.0	34.0	34.0
14	32.57	34.0	34.0	34.0	34.0	34.0
15	32.604	34.0	34.0	34.0	34.0	34.0
16	32.595	34.0	34.0	34.0	34.0	34.0
17	32.516	34.0	34.0	34.0	34.0	34.0
18	32.3985	34.0	34.0	34.0	26.0	34.0
19	32.516	34.0	34.0	34.0	34.0	34.0
20	32.507	34.0	34.0	34.0	34.0	34.0
21	32.4945	34.0	34.0	34.0	34.0	34.0
22	32.321	34.0	34.0	34.0	26.0	34.0
23	32.5385	34.0	34.0	34.0	34.0	34.0
24	32.4245	34.0	34.0	34.0	26.0	34.0
25	32.5195	34.0	34.0	34.0	34.0	34.0
26	32.5285	34.0	34.0	34.0	34.0	34.0
27	32.473	34.0	34.0	34.0	34.0	34.0
28	32.464	34.0	34.0	34.0	26.0	34.0
29	32.36	34.0	34.0	34.0	26.0	34.0
30	32.509	34.0	34.0	34.0	34.0	34.0
31	32.4475	34.0	34.0	34.0	34.0	34.0
32	32.572	34.0	34.0	34.0	34.0	34.0
33	32.3785	34.0	34.0	34.0	26.0	34.0
34	32.3085	34.0	34.0	34.0	26.0	34.0
35	32.5415	34.0	34.0	34.0	34.0	34.0
36	32.3485	34.0	34.0	34.0	26.0	34.0
37	32.3955	34.0	34.0	34.0	34.0	34.0
38	32.3985	34.0	34.0	34.0	26.0	34.0
39	32.389	34.0	34.0	34.0	26.0	34.0
40	32.2265	34.0	34.0	34.0	26.0	34.0
41	32.423	34.0	34.0	34.0	26.0	34.0
42	32.251	34.0	34.0	34.0	26.0	34.0
43	32.279	34.0	34.0	34.0	26.0	34.0
44	32.1405	34.0	34.0	34.0	26.0	34.0
45	32.293	34.0	34.0	34.0	26.0	34.0
46	32.29	34.0	34.0	34.0	26.0	34.0
47	32.2915	34.0	34.0	34.0	26.0	34.0
48	32.3485	34.0	34.0	34.0	26.0	34.0
49	32.2345	34.0	34.0	34.0	26.0	34.0
50	32.3565	34.0	34.0	34.0	26.0	34.0
51	32.1425	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	10.0
15	11.0
16	6.0
17	11.0
18	15.0
19	12.0
20	16.0
21	11.0
22	14.0
23	11.0
24	29.0
25	30.0
26	37.0
27	47.0
28	91.0
29	94.0
30	121.0
31	172.0
32	481.0
33	2093.0
34	686.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.4	23.849999999999998	4.725	32.025
2	24.9	25.5	33.4	16.2
3	21.4	22.85	32.125	23.625
4	25.624999999999996	31.175000000000004	21.95	21.25
5	29.349999999999998	33.4	19.675	17.575
6	22.525000000000002	36.0	20.9	20.575
7	22.475	21.85	35.375	20.3
8	23.025000000000002	21.725	28.299999999999997	26.950000000000003
9	21.7	22.975	29.099999999999998	26.224999999999998
10	25.900000000000002	29.75	23.549999999999997	20.8
11	30.599999999999998	24.875	19.575	24.95
12	27.450000000000003	22.875	24.6	25.074999999999996
13	26.75	25.025	25.45	22.775000000000002
14	25.424999999999997	26.875	25.575	22.125
15	26.650000000000002	26.0	23.474999999999998	23.875
16	27.825	21.7	26.400000000000002	24.075
17	27.474999999999998	25.424999999999997	23.875	23.225
18	25.650000000000002	24.575	26.05	23.724999999999998
19	27.0	24.9	24.85	23.25
20	27.224999999999998	25.825	23.575	23.375
21	26.525	24.925	25.25	23.3
22	25.75	26.625	24.975	22.650000000000002
23	27.224999999999998	26.650000000000002	23.875	22.25
24	27.175	25.35	24.474999999999998	23.0
25	25.275	25.324999999999996	25.974999999999998	23.425
26	27.625	24.675	24.975	22.725
27	25.6	25.55	26.674999999999997	22.175
28	26.950000000000003	25.15	24.875	23.025000000000002
29	27.525	25.074999999999996	23.974999999999998	23.425
30	27.775	25.05	24.6	22.575
31	26.125	23.625	25.650000000000002	24.6
32	26.875	25.674999999999997	24.224999999999998	23.225
33	26.224999999999998	25.124999999999996	25.4	23.25
34	26.325	26.1	25.275	22.3
35	26.775	25.275	25.8	22.15
36	26.6	24.25	25.525	23.625
37	24.925	23.95	27.025	24.099999999999998
38	27.325	25.2	25.05	22.425
39	25.724999999999998	24.474999999999998	25.1	24.7
40	25.474999999999998	25.7	24.725	24.099999999999998
41	27.85	25.95	22.825	23.375
42	25.624999999999996	28.349999999999998	23.65	22.375
43	25.575	26.450000000000003	23.825	24.15
44	25.674999999999997	27.575	24.224999999999998	22.525000000000002
45	25.324999999999996	26.700000000000003	23.625	24.349999999999998
46	25.1	25.775	25.55	23.575
47	27.05	26.375	24.0	22.575
48	25.8	27.200000000000003	23.875	23.125
49	25.900000000000002	24.5	25.124999999999996	24.474999999999998
50	24.8	26.674999999999997	26.325	22.2
51	27.425	24.95	24.075	23.549999999999997
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.5
12	1.0
13	1.5
14	2.0
15	1.5
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	2.0
22	3.0
23	3.0
24	3.0
25	7.5
26	7.5
27	3.0
28	13.5
29	24.0
30	25.0
31	26.0
32	38.0
33	50.0
34	92.5
35	135.0
36	154.5
37	174.0
38	194.5
39	215.0
40	241.0
41	267.0
42	292.0
43	317.0
44	307.0
45	297.0
46	304.0
47	311.0
48	339.0
49	367.0
50	341.5
51	316.0
52	305.0
53	294.0
54	266.5
55	239.0
56	228.5
57	218.0
58	222.0
59	226.0
60	189.0
61	152.0
62	137.5
63	123.0
64	101.0
65	79.0
66	58.0
67	37.0
68	31.0
69	25.0
70	15.0
71	5.0
72	3.0
73	1.0
74	2.5
75	2.0
76	0.0
77	0.5
78	1.0
79	3.0
80	5.0
81	3.0
82	1.0
83	2.5
84	4.0
85	3.0
86	2.0
87	3.0
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	3.0
94	4.0
95	3.0
96	2.0
97	6.0
98	10.0
99	20.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.62482566248256	58.525000000000006
2	10.285913528591353	14.75
3	3.486750348675035	7.5
4	1.6387726638772664	4.7
5	1.2552301255230125	4.5
6	0.6624825662482566	2.85
7	0.3486750348675035	1.7500000000000002
8	0.10460251046025104	0.6
9	0.24407252440725244	1.575
>10	0.3486750348675035	3.25
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	26	0.65	No Hit
GTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAGATCAGAATGA	14	0.35000000000000003	No Hit
AGCAAAATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCT	13	0.325	No Hit
GTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCC	13	0.325	No Hit
AGAGGATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTTA	11	0.27499999999999997	No Hit
CTGGGCTTTGTCAGCCACTTTACAGACTGGTCTTCCTGCTGGCACATACTG	11	0.27499999999999997	No Hit
CATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACACAGATG	11	0.27499999999999997	No Hit
CAATGACTGGGTCTGTGAACACAGATGGCGTCAAATCAGGAACATGGTTGC	11	0.27499999999999997	No Hit
GGGAATTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAAT	10	0.25	No Hit
GTTGGAATAGAAATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGAC	10	0.25	No Hit
CTTTCATTTTCCAAGAGGTCATTGATCTGGGTGGTGAGGCAATTAAAGGTA	9	0.22499999999999998	No Hit
GTGAACACAGATGGCGTCAAATCAGGAACATGGTTGCCTTCAGGAATGTGG	9	0.22499999999999998	No Hit
GTTACCTCAATCCAAATAACAGGGAATTCCCAGCAGTTCCATACTCTGCTT	9	0.22499999999999998	No Hit
CTTGTGTTTGTGGACAACCATGATAATCAGCGAGGACATGGTGCTGGAGGA	9	0.22499999999999998	No Hit
GTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGTTACCGTTGGAA	9	0.22499999999999998	No Hit
GATAATAACAGCAACCAAGTAGCTTTTAGCAGAGGAAACAGAGGATTCATT	9	0.22499999999999998	No Hit
GTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTCTTTAACAATGATGAC	9	0.22499999999999998	No Hit
CAGAATTCAAGTATGGTGCAAAACTTGGCACAGTTATCCGCAAGTGGAATG	8	0.2	No Hit
ATCAGAATGACTGGATTGGACCACCCAATAACAATGGAGTAACAAAAGAAG	8	0.2	No Hit
AGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGTGTGACAGAATTCA	8	0.2	No Hit
CTTCAGATGGGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCTGGGTTG	7	0.17500000000000002	No Hit
AGAATGTATAAAATGGCTGTCGGATTTATGTTGGCTCATCCTTATGGATTC	7	0.17500000000000002	No Hit
GTAATGTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAGATCAG	7	0.17500000000000002	No Hit
AGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACC	7	0.17500000000000002	No Hit
CATGAGTGCACTTCTGATCCTAGCCCTTGTGGGAGCTGCTGTTGCTTTCCC	7	0.17500000000000002	No Hit
GGGACACACCATGATCAGGACACTGCTGCTATCTGCCTTGGTGGCTGGAGC	7	0.17500000000000002	No Hit
GTCCTATTTAAAGAACTGGGGAGAAGGTTGGGGTATGGTGCCTTCTGACAG	7	0.17500000000000002	No Hit
CATAAAGGCAGTTTTGGACAAACTGCATAATCTCAATACAAAATGGTTCTC	7	0.17500000000000002	No Hit
GAATGACTGGATTGGACCACCCAATAACAATGGAGTAACAAAAGAAGTGAC	7	0.17500000000000002	No Hit
AATAACAGCAACCAAGTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTC	7	0.17500000000000002	No Hit
GCATAATCTCAATACAAAATGGTTCTCCCAAGGAAGCAGACCTTTCATTTT	6	0.15	No Hit
CACATACTTCAGATGGGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCT	6	0.15	No Hit
GAAAGATTATGTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGA	6	0.15	No Hit
CTTATGGATTCACAAGAGTAATGTCAAGTTACCGTTGGAATAGAAATTTCC	6	0.15	No Hit
AAAAGATCAGAATGACTGGATTGGACCACCCAATAACAATGGAGTAACAAA	6	0.15	No Hit
ATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATAT	6	0.15	No Hit
CTTCTGGATCTTGCACTTGAGAAAGATTATGTTCGTACCAAGGTGGCTGAC	6	0.15	No Hit
CATTAACCACATGTGTGGCGCAGGCAATCCTGCAGGAACAAGCAGTACCTG	6	0.15	No Hit
CAATAACAATGGAGTAACAAAAGAAGTGACCATTAATGCAGACACTACTTG	6	0.15	No Hit
GGTCAATGGTCAGCCTTTTTCAAACTGGTGGGATAATAACAGCAACCAAGT	6	0.15	No Hit
GAAATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACCACCCAATA	6	0.15	No Hit
GTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGACATTGGAGTA	6	0.15	No Hit
GTTCTCCCAAGGAAGCAGACCTTTCATTTTCCAAGAGGTCATTGATCTGGG	6	0.15	No Hit
AATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATA	6	0.15	No Hit
GGAAAAGATCAGAATGACTGGATTGGACCACCCAATAACAATGGAGTAACA	6	0.15	No Hit
GTCAAATCAGGAACATGGTTGCCTTCAGGAATGTGGTCAATGGTCAGCCTT	6	0.15	No Hit
GTCATTAACCACATGTGTGGCGCAGGCAATCCTGCAGGAACAAGCAGTACC	6	0.15	No Hit
GGACAAACTGCATAATCTCAATACAAAATGGTTCTCCCAAGGAAGCAGACC	6	0.15	No Hit
CCTCAATCCAAATAACAGGGAATTCCCAGCAGTTCCATACTCTGCTTGGGA	6	0.15	No Hit
CTGTGATGTCATCTCTGGAGATAAGGTCGATGGCAATTGCACTGGACTTAG	5	0.125	No Hit
ACTGGGTCTGTGAACACAGATGGCGTCAAATCAGGAACATGGTTGCCTTCA	5	0.125	No Hit
CGATGACTTCCCTGCTGGGACACTGTGTGCCACCACTGGCTGGGGCAAGAC	5	0.125	No Hit
CCCATCAAGACCTTGGTGGGAAAGATACCAACCAATCAGCTATAAAATCTG	5	0.125	No Hit
GGGCTTTGTCAGCCACTTTACAGACTGGTCTTCCTGCTGGCACATACTGTG	5	0.125	No Hit
CTCCTGCCCAGTTCTCTGAGACTGTGTCTGCCGTGTGTCTGCCCACTGTTG	5	0.125	No Hit
GTTAAATTTATTTGGTACAACAATGTGATCAACCCAACTCTACCCAAAGTG	5	0.125	No Hit
ATATGAACCATCTCATTGACATTGGAGTAGCAGGGTTCAGACTTGATGCTG	5	0.125	No Hit
AGCAGGGTTCAGACTTGATGCTGCTAAGCACATGTGGCCTGGAGACATAAA	5	0.125	No Hit
CTGGTCTTCCTGCTGGCACATACTGTGATGTCATCTCTGGAGATAAGGTCG	5	0.125	No Hit
AGGAAACAGAGGATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGC	5	0.125	No Hit
CATTGATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGG	5	0.125	No Hit
GTTCATAACCCATCAAGACCTTGGTGGGAAAGATACCAACCAATCAGCTAT	5	0.125	No Hit
GAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGA	5	0.125	No Hit
CAGTGACCCTGAGCAAGAACATCCAGACAGCTTGCCTCCCACCCGCTGGCA	5	0.125	No Hit
GTGGAAGTTACCTCAATCCAAATAACAGGGAATTCCCAGCAGTTCCATACT	5	0.125	No Hit
GCAAAATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTC	5	0.125	No Hit
GTTGGTGTCCGTATTTATGTGGATGCTGTCATTAACCACATGTGTGGCGCA	5	0.125	No Hit
GATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGT	5	0.125	No Hit
CCTCAATGCCAGAGTGGCCACTGTGGCTCTGCCCAGCTCCTGTGCACCTGC	5	0.125	No Hit
CAAGAGTAATGTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAG	5	0.125	No Hit
GTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGGAGAAATT	5	0.125	No Hit
GTTCAACTCCTTCACCGTGCGTAATGACATCACCCTGCTGAAGCTGGCCAC	5	0.125	No Hit
GATTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTTACAGA	5	0.125	No Hit
AATAGAAATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACCACCC	5	0.125	No Hit
AGTAGCTTTTAGCAGAGGAAACAGAGGATTCATTGTCTTTAACAATGATGA	5	0.125	No Hit
GGAAATGAAGATGAATTCAGAGACATGGTGACAAGGTGCAACAATGTTGGT	5	0.125	No Hit
ACTACTTGTGGCAATGACTGGGTCTGTGAACACAGATGGCGTCAAATCAGG	5	0.125	No Hit
GTCGTCTGACTGGCCTTCTGGATCTTGCACTTGAGAAAGATTATGTTCGTA	5	0.125	No Hit
ATTATGTTCGTACCAAGGTGGCTGACTATATGAACCATCTCATTGACATTG	5	0.125	No Hit
CACAGTTATCCGCAAGTGGAATGGCGAGAAGATGTCCTATTTAAAGAACTG	5	0.125	No Hit
TGTCAAGTTACCGTTGGAATAGAAATTTCCAGAATGGAAAAGATCAGAATG	5	0.125	No Hit
CCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGG	5	0.125	No Hit
CATCACCCTGCTGAAGCTGGCCACTCCTGCCCAGTTCTCTGAGACTGTGTC	5	0.125	No Hit
CTTGATGCTGCTAAGCACATGTGGCCTGGAGACATAAAGGCAGTTTTGGAC	5	0.125	No Hit
GTTATCCGCAAGTGGAATGGCGAGAAGATGTCCTATTTAAAGAACTGGGGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
Read 1159231 spots for SRR24820675.sra
Written 1159231 spots for SRR24820675.sra
SRR ids: ['SRR24820675.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tsvibo36
SRR24820675.sra spots: 9273848
blocks: [[1, 1159231], [1159232, 2318462], [2318463, 3477693], [3477694, 4636924], [4636925, 5796155], [5796156, 6955386], [6955387, 8114617], [8114618, 9273848]]
SRR24820675 file size 1591348
SRR24820675 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24820675 SRR24820675_1.fastq SRR24820675_2.fastq
Input file:	SRR24820675_1.fastq
Paired file:	SRR24820675_2.fastq
trimmed:	SRR24820675-trimmed-pair1.fastq, SRR24820675-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun Jul  5 10:18:52 2026 >> started

Sun Jul  5 10:18:59 2026 >> done (6.720s)
9273848 read pairs processed; of these:
    538 ( 0.01%) short read pairs filtered out after trimming by size control
   6773 ( 0.07%) empty read pairs filtered out after trimming by size control
9266537 (99.92%) read pairs available; of these:
   8534 ( 0.09%) trimmed read pairs available after processing
9258003 (99.91%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     24	  0.00%
 19	      9	  0.00%
 20	     22	  0.00%
 21	     10	  0.00%
 22	     19	  0.00%
 23	     17	  0.00%
 24	     23	  0.00%
 25	     61	  0.00%
 26	     22	  0.00%
 27	     19	  0.00%
 28	     20	  0.00%
 29	     19	  0.00%
 30	     23	  0.00%
 31	     30	  0.00%
 32	     46	  0.00%
 33	     56	  0.00%
 34	     56	  0.00%
 35	     77	  0.00%
 36	    104	  0.00%
 37	    141	  0.00%
 38	    219	  0.00%
 39	    219	  0.00%
 40	    311	  0.00%
 41	    379	  0.00%
 42	    427	  0.00%
 43	    434	  0.00%
 44	    504	  0.01%
 45	    468	  0.01%
 46	    586	  0.01%
 47	    802	  0.01%
 48	    932	  0.01%
 49	   1142	  0.01%
 50	   1313	  0.01%
 51	9258003	 99.91%
9266537 reads passed initial QC


criterion=sequence-density
sequence-density=1.21
sequence-density-rank=1
fanout-score=3.34
fanout-score-rank=10
prefix-density=1.52
prefix-fanout=2.6
sequence=TTGCCCCAGCCAG


criterion=fanout-score
sequence-density=0.59
sequence-density-rank=3
fanout-score=11.10
fanout-score-rank=1
prefix-density=1.82
prefix-fanout=3.6
sequence=GCCCCAGCCAGTGGTGGCACACAGTGTCCCAGCAGGGAAGTCATCGTCAACAGTGGGCAGACACACGGCAGACACAGTCTCAGAGAACTGGGCAGGAGTGGCCAGCTTCAGCAGGGTGATGTCATTACGCACGGTGAAGGAGTTGAACTTGGGGTTCTTAAAAACCTGAGCGATTTTCAGGACCTGGACATTCTCTTCATCGGAGCCCTGATCAAACTCTCCAGCTACCACCACATCGGTTGTCTTGACCCCGCAGTGGGCAGCAGTGACCACCCAGTTTTCGCTGATGAGGGAGCCCCCGCAGAAATGGAAGCCAGTTCTGTCCTGCAGGGACACCT


criterion=sequence-density
sequence-density=0.88
sequence-density-rank=1
fanout-score=7.78
fanout-score-rank=4
prefix-density=2.06
prefix-fanout=3.3
sequence=GCCCTTGTGGGAGCTGCTGTTGCTTTCCCTGTGGATGATGATGACAAGAT


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=29
fanout-score=15.80
fanout-score-rank=1
prefix-density=0.56
prefix-fanout=3.0
sequence=GTGCTGCTGGGAGCAGTAGCTGGAAGGGAAGTTTGCTTCGACAAACTCGGCTGCTTCAGTGATGATGCCCCCTGGTCAGGAACTCTAGACAGACCCCTGAAAGCACTGCCCTGGAGCCCAGCACAGATCAACACCCGCTTTCTCCTCTACACCAACGAGAATCCAGACAACTACCAGCTAATTACTTCAGATGCATCAAACATCAGAAACTCCAATTTCCGAACAAACAGAAAAACCCGTATCATTATCCACGGCTTCATCGACAAGGGAGAAGAAAACTGGCTATCTGACATGTGCAAGAACATGTTCCGAGTGGAGAGTGTGAACTGCATCTGTGTGGACTGGAAAGGCGGTTCCCGAACGACATATACCCAGGCTACCCAGAACGTCCGGGTCGTGGGGGCAGAAGTGGCATTGTTGGTTAACGTTCTGCAGTCTGATCTGGGATACTCACTTAACAATGTCCACCTGATTGGCCACAGCCTGGGTTCCCACATTGCTGGGGAGGCTG
SRR24820675 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 05 10:19:16
                             Started mapping on |	Jul 05 10:19:16
                                    Finished on |	Jul 05 10:20:34
       Mapping speed, Million of reads per hour |	427.69

                          Number of input reads |	9266537
                      Average input read length |	101
                                    UNIQUE READS:
                   Uniquely mapped reads number |	5058625
                        Uniquely mapped reads % |	54.59%
                          Average mapped length |	101.68
                       Number of splices: Total |	2690678
            Number of splices: Annotated (sjdb) |	2687836
                       Number of splices: GT/AG |	2686036
                       Number of splices: GC/AG |	3811
                       Number of splices: AT/AC |	234
               Number of splices: Non-canonical |	597
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.59
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.15
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2780578
             % of reads mapped to multiple loci |	30.01%
        Number of reads mapped to too many loci |	954240
             % of reads mapped to too many loci |	10.30%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.09%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1427334	1427334	1427334
N_multimapping	2780578	2780578	2780578
N_noFeature	157395	4931726	183869
N_ambiguous	215926	4973	187973
UnstrandedReadsAssigned:4685304 PositiveStrandReadsAssigned:121926 NegativeStrandReadsAssigned:4686783
Dataset is classified negative stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR24820675 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24820675-trimmed-pair1.fastq
                             SRR24820675-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 9,266,537 reads, 8,683,554 reads pseudoaligned
[quant] estimated average fragment length: 150.158
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 929 rounds

 109283 SRR24820675.ke.tsv
  52636 SRR24820675.se.tsv
 161919 total
==> SRR24820675.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR24820675.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24820675 completed mapping pipeline successfully
