Starting /dee2/code/volunteer_pipeline.sh SRR24882416
    current disk space = 438571476992
    free memory = 323150024 
SRR24882416 SRAfilesize
07d48825bbd8f17965a87a34298e5c8b  SRR24882416.sra
SRR24882416.sra file validated
SRR24882416 is paired end
SRR24882416 is conventional basespace
SRR24882416 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5585	37.0	37.0	37.0	37.0	37.0
2	36.4755	37.0	37.0	37.0	37.0	37.0
3	36.477	37.0	37.0	37.0	37.0	37.0
4	36.627	37.0	37.0	37.0	37.0	37.0
5	36.606	37.0	37.0	37.0	37.0	37.0
6	36.6655	37.0	37.0	37.0	37.0	37.0
7	36.5705	37.0	37.0	37.0	37.0	37.0
8	36.5755	37.0	37.0	37.0	37.0	37.0
9	36.5915	37.0	37.0	37.0	37.0	37.0
10-11	36.61775	37.0	37.0	37.0	37.0	37.0
12-13	36.60425	37.0	37.0	37.0	37.0	37.0
14-15	36.554500000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.66075	37.0	37.0	37.0	37.0	37.0
18-19	36.527249999999995	37.0	37.0	37.0	37.0	37.0
20-21	36.60425	37.0	37.0	37.0	37.0	37.0
22-23	36.593	37.0	37.0	37.0	37.0	37.0
24-25	36.567750000000004	37.0	37.0	37.0	37.0	37.0
26-27	36.54475	37.0	37.0	37.0	37.0	37.0
28-29	36.56875	37.0	37.0	37.0	37.0	37.0
30-31	36.494749999999996	37.0	37.0	37.0	37.0	37.0
32-33	36.4965	37.0	37.0	37.0	37.0	37.0
34-35	36.554249999999996	37.0	37.0	37.0	37.0	37.0
36-37	36.429	37.0	37.0	37.0	37.0	37.0
38-39	36.5145	37.0	37.0	37.0	37.0	37.0
40-41	36.429500000000004	37.0	37.0	37.0	37.0	37.0
42-43	36.5215	37.0	37.0	37.0	37.0	37.0
44-45	36.45025	37.0	37.0	37.0	37.0	37.0
46-47	36.52225	37.0	37.0	37.0	37.0	37.0
48-49	36.4165	37.0	37.0	37.0	37.0	37.0
50-51	36.46425	37.0	37.0	37.0	37.0	37.0
52-53	36.43175	37.0	37.0	37.0	37.0	37.0
54-55	36.434375	37.0	37.0	37.0	37.0	37.0
56-57	36.349875	37.0	37.0	37.0	37.0	37.0
58-59	36.4745	37.0	37.0	37.0	37.0	37.0
60-61	36.364000000000004	37.0	37.0	37.0	37.0	37.0
62-63	36.36725	37.0	37.0	37.0	37.0	37.0
64-65	36.368	37.0	37.0	37.0	37.0	37.0
66-67	36.40775	37.0	37.0	37.0	37.0	37.0
68-69	36.267250000000004	37.0	37.0	37.0	37.0	37.0
70-71	36.299	37.0	37.0	37.0	37.0	37.0
72-73	36.370875	37.0	37.0	37.0	37.0	37.0
74-75	36.312	37.0	37.0	37.0	37.0	37.0
76-77	36.320750000000004	37.0	37.0	37.0	37.0	37.0
78-79	36.2425	37.0	37.0	37.0	37.0	37.0
80-81	36.2775	37.0	37.0	37.0	37.0	37.0
82-83	36.26375	37.0	37.0	37.0	37.0	37.0
84-85	36.31325	37.0	37.0	37.0	37.0	37.0
86-87	36.28275	37.0	37.0	37.0	37.0	37.0
88-89	36.197	37.0	37.0	37.0	37.0	37.0
90-91	36.21725	37.0	37.0	37.0	37.0	37.0
92-93	36.171125	37.0	37.0	37.0	37.0	37.0
94-95	36.1455	37.0	37.0	37.0	37.0	37.0
96-97	36.08675	37.0	37.0	37.0	37.0	37.0
98-99	36.157375	37.0	37.0	37.0	37.0	37.0
100-101	35.924	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	4.0
26	2.0
27	7.0
28	7.0
29	13.0
30	15.0
31	23.0
32	29.0
33	58.0
34	76.0
35	264.0
36	2719.0
37	783.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.35	11.450000000000001	11.4	40.8
2	19.35	17.2	34.8	28.65
3	18.41841841841842	20.92092092092092	32.707707707707705	27.952952952952952
4	25.674999999999997	27.725	24.075	22.525000000000002
5	24.525	31.424999999999997	23.45	20.599999999999998
6	19.475	35.8	27.800000000000004	16.925
7	14.899999999999999	27.500000000000004	43.35	14.249999999999998
8	21.3	23.9	31.574999999999996	23.225
9	19.075	24.775	33.6	22.55
10-11	19.8875	35.6375	24.9875	19.4875
12-13	23.7125	25.7625	27.025	23.5
14-15	20.4875	31.0125	28.1625	20.3375
16-17	21.1875	30.175	24.3125	24.325
18-19	23.6125	30.125	24.2375	22.025
20-21	22.75	28.237499999999997	27.462500000000002	21.55
22-23	23.05	28.762500000000003	25.362499999999997	22.825
24-25	23.849999999999998	30.7	24.775	20.674999999999997
26-27	22.275	30.049999999999997	26.900000000000002	20.775
28-29	19.525000000000002	31.55	28.050000000000004	20.875
30-31	21.175	26.8625	29.225	22.7375
32-33	20.150000000000002	27.8375	29.675	22.3375
34-35	17.2	28.4125	28.7375	25.650000000000002
36-37	21.85	28.499999999999996	30.362499999999997	19.287499999999998
38-39	19.75	29.5	30.775000000000002	19.975
40-41	20.325	30.887500000000003	28.325	20.4625
42-43	21.7375	32.9	25.650000000000002	19.7125
44-45	18.087500000000002	33.387499999999996	25.474999999999998	23.05
46-47	22.775000000000002	33.7625	22.3875	21.075
48-49	20.525	34.425	24.775	20.275000000000002
50-51	22.0125	29.3875	25.924999999999997	22.675
52-53	21.15	27.037499999999998	27.925	23.8875
54-55	22.015251906488313	26.990873859232405	27.753469183647955	23.24040505063133
56-57	18.277284660582573	31.278909863732967	27.57844730591324	22.86535816977122
58-59	20.125	28.675	27.537499999999998	23.6625
60-61	19.7125	32.7	24.837500000000002	22.75
62-63	24.6625	30.0875	25.25	20.0
64-65	23.525	26.55	27.425	22.5
66-67	23.2625	28.512500000000003	27.3	20.925
68-69	21.7375	29.75	26.5875	21.925
70-71	23.075000000000003	28.875	27.3125	20.7375
72-73	19.31491436429554	31.716464558069756	27.353419177397175	21.61520190023753
74-75	22.912499999999998	28.512500000000003	27.8125	20.7625
76-77	24.4875	28.95	24.675	21.8875
78-79	24.4875	26.825	27.750000000000004	20.9375
80-81	26.325	26.0	28.050000000000004	19.625
82-83	24.2375	27.750000000000004	27.787499999999998	20.225
84-85	19.975	30.312499999999996	26.5875	23.125
86-87	22.0875	28.65	27.275	21.987499999999997
88-89	24.95	28.000000000000004	28.575	18.475
90-91	23.4125	27.975	27.925	20.6875
92-93	22.027753469183647	28.291036379547442	27.790973871733964	21.890236279534943
94-95	22.2125	30.912499999999998	26.125	20.75
96-97	20.0625	31.0625	22.325	26.55
98-99	21.352669083635455	29.391173896737094	27.628453556694588	21.627703462932867
100-101	22.75	29.15	27.575	20.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	4.0
22	9.5
23	10.5
24	6.0
25	17.5
26	25.5
27	10.5
28	7.0
29	16.5
30	36.5
31	57.0
32	105.5
33	153.5
34	151.5
35	172.5
36	154.0
37	120.5
38	83.0
39	49.5
40	88.5
41	179.5
42	269.0
43	220.5
44	139.5
45	130.5
46	215.0
47	288.5
48	214.0
49	126.5
50	103.0
51	94.0
52	68.5
53	40.0
54	41.5
55	62.0
56	71.0
57	50.0
58	55.0
59	112.5
60	94.5
61	33.5
62	28.5
63	20.0
64	7.5
65	5.5
66	2.0
67	7.0
68	8.0
69	3.5
70	3.0
71	2.5
72	1.5
73	2.0
74	6.0
75	5.5
76	1.5
77	2.0
78	3.0
79	2.0
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.1
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0125
56-57	0.0125
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0125
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0
98-99	0.0125
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.074999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.19501133786848	17.925
2	16.099773242630384	10.65
3	8.16326530612245	8.1
4	5.215419501133787	6.9
5	3.4769463340891913	5.75
6	2.3431594860166287	4.65
7	1.5117157974300832	3.5000000000000004
8	1.2849584278155708	3.4000000000000004
9	1.6628873771730914	4.95
>10	5.971277399848828	32.525
>50	0.07558578987150416	1.6500000000000001
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	66	1.6500000000000001	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	45	1.125	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	41	1.0250000000000001	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	39	0.975	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	32	0.8	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	31	0.775	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	30	0.75	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	24	0.6	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	24	0.6	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	23	0.575	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	22	0.5499999999999999	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	22	0.5499999999999999	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	21	0.525	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	21	0.525	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	21	0.525	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	21	0.525	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	20	0.5	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	19	0.475	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	19	0.475	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	18	0.44999999999999996	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	18	0.44999999999999996	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	18	0.44999999999999996	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	18	0.44999999999999996	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	18	0.44999999999999996	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	17	0.42500000000000004	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	17	0.42500000000000004	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	17	0.42500000000000004	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	17	0.42500000000000004	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	17	0.42500000000000004	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	17	0.42500000000000004	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	17	0.42500000000000004	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	17	0.42500000000000004	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	16	0.4	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	16	0.4	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	16	0.4	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	16	0.4	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	16	0.4	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	16	0.4	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	16	0.4	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	15	0.375	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	15	0.375	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	15	0.375	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	15	0.375	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	15	0.375	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	14	0.35000000000000003	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	14	0.35000000000000003	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	14	0.35000000000000003	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	14	0.35000000000000003	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	14	0.35000000000000003	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	13	0.325	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	13	0.325	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	13	0.325	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	13	0.325	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	13	0.325	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	12	0.3	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	12	0.3	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	12	0.3	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	12	0.3	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	11	0.27499999999999997	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	11	0.27499999999999997	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	11	0.27499999999999997	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	11	0.27499999999999997	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	11	0.27499999999999997	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	11	0.27499999999999997	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	11	0.27499999999999997	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	11	0.27499999999999997	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	11	0.27499999999999997	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	11	0.27499999999999997	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	10	0.25	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	10	0.25	No Hit
AGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATC	10	0.25	No Hit
CACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTAT	10	0.25	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	10	0.25	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	10	0.25	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	10	0.25	No Hit
CAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACACACAC	10	0.25	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	10	0.25	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	10	0.25	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	10	0.25	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	10	0.25	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	9	0.22499999999999998	No Hit
GTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACAC	9	0.22499999999999998	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	9	0.22499999999999998	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	9	0.22499999999999998	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	9	0.22499999999999998	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	9	0.22499999999999998	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	9	0.22499999999999998	No Hit
GCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTAG	9	0.22499999999999998	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	9	0.22499999999999998	No Hit
CCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATC	9	0.22499999999999998	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	9	0.22499999999999998	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	9	0.22499999999999998	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	9	0.22499999999999998	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	9	0.22499999999999998	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	9	0.22499999999999998	No Hit
AAATAAATCATTGGTAAACATTACAAAATTAAATACATTTTCAAAAGCTT	9	0.22499999999999998	No Hit
CCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTGGC	9	0.22499999999999998	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	9	0.22499999999999998	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	9	0.22499999999999998	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	9	0.22499999999999998	No Hit
GCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGA	9	0.22499999999999998	No Hit
GAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTC	9	0.22499999999999998	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	8	0.2	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	8	0.2	No Hit
GCTCCCTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATT	8	0.2	No Hit
GGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	8	0.2	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	8	0.2	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	8	0.2	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	8	0.2	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	8	0.2	No Hit
TCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCT	8	0.2	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	8	0.2	No Hit
GGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTAC	8	0.2	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	8	0.2	No Hit
GTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATA	8	0.2	No Hit
CCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCA	8	0.2	No Hit
GGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGC	8	0.2	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	8	0.2	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	8	0.2	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	7	0.17500000000000002	No Hit
TTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTG	7	0.17500000000000002	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	7	0.17500000000000002	No Hit
TTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTG	7	0.17500000000000002	No Hit
CGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTAC	7	0.17500000000000002	No Hit
TGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTAC	7	0.17500000000000002	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	7	0.17500000000000002	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	7	0.17500000000000002	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	7	0.17500000000000002	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	7	0.17500000000000002	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	7	0.17500000000000002	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	7	0.17500000000000002	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	7	0.17500000000000002	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	7	0.17500000000000002	No Hit
CTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGAACAT	7	0.17500000000000002	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	7	0.17500000000000002	No Hit
GATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATT	7	0.17500000000000002	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	7	0.17500000000000002	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	7	0.17500000000000002	No Hit
GAAATAATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACATATGC	7	0.17500000000000002	No Hit
CCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAA	6	0.15	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	6	0.15	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	6	0.15	No Hit
ATTGGTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATT	6	0.15	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	6	0.15	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	6	0.15	No Hit
CTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAA	6	0.15	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	6	0.15	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	6	0.15	No Hit
CCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAG	6	0.15	No Hit
CCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGA	6	0.15	No Hit
CATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTT	6	0.15	No Hit
AGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTA	6	0.15	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	6	0.15	No Hit
GACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTG	6	0.15	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	6	0.15	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	6	0.15	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	6	0.15	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	6	0.15	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	6	0.15	No Hit
ATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCT	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	6	0.15	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	6	0.15	No Hit
GGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTT	6	0.15	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	6	0.15	No Hit
CTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATAT	6	0.15	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	6	0.15	No Hit
AGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGC	6	0.15	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	6	0.15	No Hit
GCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCT	6	0.15	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	6	0.15	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	5	0.125	No Hit
GGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAG	5	0.125	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	5	0.125	No Hit
CACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTT	5	0.125	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	5	0.125	No Hit
CTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCT	5	0.125	No Hit
CCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGC	5	0.125	No Hit
ATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTC	5	0.125	No Hit
GTCAATTTCTACATCTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGAC	5	0.125	No Hit
TAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTC	5	0.125	No Hit
GGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAAC	5	0.125	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	5	0.125	No Hit
CTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTA	5	0.125	No Hit
GAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATAT	5	0.125	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	5	0.125	No Hit
CAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATG	5	0.125	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	5	0.125	No Hit
CTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGG	5	0.125	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	5	0.125	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	5	0.125	No Hit
TATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACT	5	0.125	No Hit
AAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCT	5	0.125	No Hit
ACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAA	5	0.125	No Hit
TTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAAT	5	0.125	No Hit
TAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACA	5	0.125	No Hit
GCTCTTTCCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTC	5	0.125	No Hit
TCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAG	5	0.125	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	5	0.125	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	5	0.125	No Hit
CACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTC	5	0.125	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	5	0.125	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	5	0.125	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	5	0.125	No Hit
AGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTC	5	0.125	No Hit
TTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAA	5	0.125	No Hit
CTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCA	5	0.125	No Hit
GGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTG	5	0.125	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	5	0.125	No Hit
CTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAGAAA	5	0.125	No Hit
TTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGT	5	0.125	No Hit
GGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGA	5	0.125	No Hit
GGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGGCA	5	0.125	No Hit
ATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGG	5	0.125	No Hit
ATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAA	5	0.125	No Hit
CCTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATA	5	0.125	No Hit
CTGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.125	0.0	0.0	0.0	0.0
76-77	0.2	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.2875	0.0	0.0	0.0	0.0
82-83	0.3625	0.0	0.0	0.0	0.0
84-85	0.4875	0.0	0.0	0.0	0.0
86-87	0.5375000000000001	0.0	0.0	0.0	0.0
88-89	0.6875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882416 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882416_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.40725	37.0	37.0	37.0	37.0	37.0
2	36.236	37.0	37.0	37.0	37.0	37.0
3	36.274	37.0	37.0	37.0	37.0	37.0
4	36.416	37.0	37.0	37.0	37.0	37.0
5	36.4415	37.0	37.0	37.0	37.0	37.0
6	36.403	37.0	37.0	37.0	37.0	37.0
7	36.44175	37.0	37.0	37.0	37.0	37.0
8	36.42	37.0	37.0	37.0	37.0	37.0
9	36.3955	37.0	37.0	37.0	37.0	37.0
10-11	36.452	37.0	37.0	37.0	37.0	37.0
12-13	36.487750000000005	37.0	37.0	37.0	37.0	37.0
14-15	36.42775	37.0	37.0	37.0	37.0	37.0
16-17	36.412000000000006	37.0	37.0	37.0	37.0	37.0
18-19	36.465	37.0	37.0	37.0	37.0	37.0
20-21	36.3775	37.0	37.0	37.0	37.0	37.0
22-23	36.2845	37.0	37.0	37.0	37.0	37.0
24-25	36.32725	37.0	37.0	37.0	37.0	37.0
26-27	36.25075	37.0	37.0	37.0	37.0	37.0
28-29	36.3105	37.0	37.0	37.0	37.0	37.0
30-31	36.3505	37.0	37.0	37.0	37.0	37.0
32-33	36.272000000000006	37.0	37.0	37.0	37.0	37.0
34-35	36.345749999999995	37.0	37.0	37.0	37.0	37.0
36-37	36.30875	37.0	37.0	37.0	37.0	37.0
38-39	36.2915	37.0	37.0	37.0	37.0	37.0
40-41	36.293499999999995	37.0	37.0	37.0	37.0	37.0
42-43	36.271	37.0	37.0	37.0	37.0	37.0
44-45	36.192499999999995	37.0	37.0	37.0	37.0	37.0
46-47	36.152	37.0	37.0	37.0	37.0	37.0
48-49	36.08975	37.0	37.0	37.0	37.0	37.0
50-51	36.044	37.0	37.0	37.0	37.0	37.0
52-53	36.075	37.0	37.0	37.0	37.0	37.0
54-55	36.02825	37.0	37.0	37.0	37.0	37.0
56-57	36.09725	37.0	37.0	37.0	37.0	37.0
58-59	36.137375000000006	37.0	37.0	37.0	37.0	37.0
60-61	36.02525	37.0	37.0	37.0	37.0	37.0
62-63	36.08675	37.0	37.0	37.0	37.0	37.0
64-65	36.1045	37.0	37.0	37.0	37.0	37.0
66-67	36.0995	37.0	37.0	37.0	37.0	37.0
68-69	35.96125	37.0	37.0	37.0	37.0	37.0
70-71	35.92825	37.0	37.0	37.0	37.0	37.0
72-73	35.953500000000005	37.0	37.0	37.0	37.0	37.0
74-75	35.9285	37.0	37.0	37.0	37.0	37.0
76-77	35.963499999999996	37.0	37.0	37.0	37.0	37.0
78-79	35.990750000000006	37.0	37.0	37.0	37.0	37.0
80-81	35.8925	37.0	37.0	37.0	37.0	37.0
82-83	35.92	37.0	37.0	37.0	37.0	37.0
84-85	35.873999999999995	37.0	37.0	37.0	37.0	37.0
86-87	35.7635	37.0	37.0	37.0	37.0	37.0
88-89	35.72825	37.0	37.0	37.0	37.0	37.0
90-91	35.61525	37.0	37.0	37.0	37.0	37.0
92-93	35.693	37.0	37.0	37.0	37.0	37.0
94-95	35.712	37.0	37.0	37.0	37.0	37.0
96-97	35.7255	37.0	37.0	37.0	37.0	37.0
98-99	35.803250000000006	37.0	37.0	37.0	37.0	37.0
100-101	35.4995	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	1.0
23	1.0
24	0.0
25	4.0
26	5.0
27	8.0
28	15.0
29	10.0
30	20.0
31	32.0
32	38.0
33	74.0
34	148.0
35	595.0
36	2726.0
37	322.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.21863260706236	19.133483596293512	15.52717255196594	26.120711244678184
2	19.15	26.525	37.0	17.325
3	19.475	25.974999999999998	34.1	20.45
4	23.225	32.45	24.15	20.175
5	25.724999999999998	31.85	26.224999999999998	16.2
6	23.799999999999997	32.9	22.95	20.349999999999998
7	19.879969992498125	21.380345086271568	39.30982745686421	19.42985746436609
8	23.075000000000003	21.65	30.2	25.074999999999996
9	21.325	25.0	31.95	21.725
10-11	24.125	31.25	23.875	20.75
12-13	25.874999999999996	24.875	28.0875	21.1625
14-15	23.3125	25.0625	31.087500000000002	20.5375
16-17	23.875	27.6875	30.55	17.8875
18-19	22.9875	26.3125	29.549999999999997	21.15
20-21	22.15	27.200000000000003	32.1625	18.4875
22-23	20.875	28.0875	29.3375	21.7
24-25	23.7125	27.125	29.1875	19.975
26-27	23.175	27.212500000000002	27.750000000000004	21.8625
28-29	22.662499999999998	27.450000000000003	29.799999999999997	20.0875
30-31	23.4625	27.325	28.849999999999998	20.3625
32-33	23.3375	25.95	30.675	20.0375
34-35	22.6875	27.3875	26.6	23.325000000000003
36-37	21.7375	28.6625	29.7875	19.8125
38-39	21.825	26.987499999999997	28.15	23.0375
40-41	20.875	27.6625	30.2375	21.224999999999998
42-43	21.575	29.275000000000002	27.925	21.224999999999998
44-45	21.2625	27.6875	31.2875	19.7625
46-47	22.1	27.250000000000004	27.3125	23.3375
48-49	22.112499999999997	26.437500000000004	30.862499999999997	20.5875
50-51	21.1125	27.075	28.799999999999997	23.0125
52-53	22.625	26.375	31.125000000000004	19.875
54-55	25.7	25.8125	28.037499999999998	20.45
56-57	19.825	28.050000000000004	29.725	22.400000000000002
58-59	20.42755344418052	27.55344418052256	29.353669208651077	22.665333166645834
60-61	19.4875	28.6875	28.9125	22.912499999999998
62-63	22.05	27.900000000000002	27.6	22.45
64-65	22.5	26.075	31.225	20.200000000000003
66-67	20.3875	24.925	30.15	24.5375
68-69	22.4875	28.0625	28.999999999999996	20.45
70-71	21.1875	28.349999999999998	29.912499999999998	20.549999999999997
72-73	21.6625	27.0875	28.7	22.55
74-75	22.5875	25.75	29.9625	21.7
76-77	22.650000000000002	27.9125	28.025	21.4125
78-79	21.8625	26.4125	31.2	20.525
80-81	21.75	27.375	30.65	20.225
82-83	19.9125	26.5875	30.4375	23.0625
84-85	19.3375	27.500000000000004	30.425	22.7375
86-87	21.4375	28.0625	27.4125	23.0875
88-89	22.650000000000002	27.537499999999998	28.9	20.9125
90-91	22.287499999999998	27.4125	27.700000000000003	22.6
92-93	22.787499999999998	28.6125	28.5625	20.0375
94-95	22.075	30.0875	27.1	20.7375
96-97	23.0625	27.487499999999997	28.000000000000004	21.45
98-99	21.3875	27.474999999999998	28.3625	22.775000000000002
100-101	21.525	27.700000000000003	28.775000000000002	22.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	0.5
27	1.0
28	4.0
29	11.5
30	52.0
31	121.0
32	161.5
33	142.5
34	123.5
35	103.5
36	101.0
37	149.5
38	139.5
39	101.0
40	118.5
41	162.0
42	218.0
43	238.5
44	242.0
45	254.5
46	226.5
47	224.5
48	184.0
49	111.5
50	96.0
51	82.0
52	58.5
53	34.0
54	41.0
55	95.0
56	80.5
57	25.0
58	48.0
59	54.5
60	26.5
61	16.0
62	23.0
63	19.0
64	5.0
65	2.5
66	1.0
67	5.5
68	6.5
69	5.5
70	7.0
71	8.0
72	7.5
73	5.0
74	7.5
75	10.5
76	11.5
77	14.0
78	9.5
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0125
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.275000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.38095238095239	19.525000000000002
2	17.236753856472166	12.85
3	10.46277665995976	11.700000000000001
4	5.767940979208585	8.6
5	3.420523138832998	6.375
6	2.1462105969148224	4.8
7	1.4084507042253522	3.675
8	1.6096579476861168	4.8
9	1.341381623071764	4.5
>10	4.225352112676056	23.175
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	37	0.9249999999999999	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	36	0.8999999999999999	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	31	0.775	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	24	0.6	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	22	0.5499999999999999	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	22	0.5499999999999999	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	21	0.525	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	21	0.525	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	21	0.525	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	20	0.5	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	19	0.475	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	19	0.475	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	19	0.475	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	18	0.44999999999999996	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	18	0.44999999999999996	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	18	0.44999999999999996	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	17	0.42500000000000004	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	16	0.4	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	16	0.4	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	15	0.375	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	15	0.375	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	14	0.35000000000000003	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	14	0.35000000000000003	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	14	0.35000000000000003	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	13	0.325	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	13	0.325	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	13	0.325	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	13	0.325	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	13	0.325	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	13	0.325	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	13	0.325	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	12	0.3	No Hit
GTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACT	12	0.3	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	12	0.3	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	12	0.3	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	12	0.3	No Hit
CTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTC	12	0.3	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	12	0.3	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	12	0.3	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	12	0.3	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	11	0.27499999999999997	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	11	0.27499999999999997	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	11	0.27499999999999997	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	11	0.27499999999999997	No Hit
GTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAG	11	0.27499999999999997	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	11	0.27499999999999997	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	11	0.27499999999999997	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	11	0.27499999999999997	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	11	0.27499999999999997	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	11	0.27499999999999997	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	11	0.27499999999999997	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	10	0.25	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	10	0.25	No Hit
AGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTC	10	0.25	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	10	0.25	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	10	0.25	No Hit
GGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTC	10	0.25	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	10	0.25	No Hit
TTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGAT	10	0.25	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	10	0.25	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	10	0.25	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	10	0.25	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	10	0.25	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	9	0.22499999999999998	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	9	0.22499999999999998	No Hit
GTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTT	9	0.22499999999999998	No Hit
CAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAG	9	0.22499999999999998	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	9	0.22499999999999998	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	9	0.22499999999999998	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	9	0.22499999999999998	No Hit
CTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAA	9	0.22499999999999998	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	9	0.22499999999999998	No Hit
GAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAA	9	0.22499999999999998	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	9	0.22499999999999998	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	9	0.22499999999999998	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	9	0.22499999999999998	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	9	0.22499999999999998	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	9	0.22499999999999998	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	9	0.22499999999999998	No Hit
GTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCT	9	0.22499999999999998	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	9	0.22499999999999998	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	9	0.22499999999999998	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	9	0.22499999999999998	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	8	0.2	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	8	0.2	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	8	0.2	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	8	0.2	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	8	0.2	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	8	0.2	No Hit
CCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCT	8	0.2	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	8	0.2	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	8	0.2	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	8	0.2	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	8	0.2	No Hit
CTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTCA	8	0.2	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	8	0.2	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	8	0.2	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	8	0.2	No Hit
GAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATA	8	0.2	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	8	0.2	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	8	0.2	No Hit
CAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGA	8	0.2	No Hit
CTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGT	8	0.2	No Hit
GTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTG	8	0.2	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	8	0.2	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	8	0.2	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	8	0.2	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	7	0.17500000000000002	No Hit
CTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTG	7	0.17500000000000002	No Hit
ATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGT	7	0.17500000000000002	No Hit
TTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGT	7	0.17500000000000002	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	7	0.17500000000000002	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	7	0.17500000000000002	No Hit
GGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAG	7	0.17500000000000002	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	7	0.17500000000000002	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	7	0.17500000000000002	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	7	0.17500000000000002	No Hit
GGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGC	7	0.17500000000000002	No Hit
GACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCT	7	0.17500000000000002	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	7	0.17500000000000002	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	7	0.17500000000000002	No Hit
CCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTAC	7	0.17500000000000002	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	7	0.17500000000000002	No Hit
TCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGT	7	0.17500000000000002	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	7	0.17500000000000002	No Hit
CCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACC	7	0.17500000000000002	No Hit
TTACAATGAGCCATACTGCATATGTGTTGAGTTGGTCAAGGGCTTAATTC	7	0.17500000000000002	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	7	0.17500000000000002	No Hit
ATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTG	6	0.15	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	6	0.15	No Hit
GAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAA	6	0.15	No Hit
GTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAG	6	0.15	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	6	0.15	No Hit
GGCGGCCGGTGGCGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTC	6	0.15	No Hit
CTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCA	6	0.15	No Hit
CTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGA	6	0.15	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	6	0.15	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	6	0.15	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	6	0.15	No Hit
TTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATC	6	0.15	No Hit
GAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGA	6	0.15	No Hit
GCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAA	6	0.15	No Hit
CCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTT	6	0.15	No Hit
GGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGA	6	0.15	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	6	0.15	No Hit
TTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACAC	6	0.15	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	6	0.15	No Hit
CAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATG	6	0.15	No Hit
TGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTGAACACAGT	6	0.15	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	6	0.15	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	6	0.15	No Hit
GTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATG	6	0.15	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	6	0.15	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	6	0.15	No Hit
GCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGAT	6	0.15	No Hit
CTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGT	6	0.15	No Hit
GTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTA	6	0.15	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	6	0.15	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	6	0.15	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	6	0.15	No Hit
CAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGT	5	0.125	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	5	0.125	No Hit
AGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTA	5	0.125	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	5	0.125	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	5	0.125	No Hit
AAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACAC	5	0.125	No Hit
CATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAA	5	0.125	No Hit
CTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTGA	5	0.125	No Hit
AAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTG	5	0.125	No Hit
TGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGT	5	0.125	No Hit
TCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTT	5	0.125	No Hit
AATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAAT	5	0.125	No Hit
CTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATG	5	0.125	No Hit
ATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCT	5	0.125	No Hit
TAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTT	5	0.125	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	5	0.125	No Hit
GTGTAATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAAC	5	0.125	No Hit
ATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCT	5	0.125	No Hit
TGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGAC	5	0.125	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	5	0.125	No Hit
CCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAA	5	0.125	No Hit
TAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTAC	5	0.125	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	5	0.125	No Hit
TCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTG	5	0.125	No Hit
AATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGT	5	0.125	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	5	0.125	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	5	0.125	No Hit
ATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCA	5	0.125	No Hit
CCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAGC	5	0.125	No Hit
GCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTG	5	0.125	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	5	0.125	No Hit
GATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGC	5	0.125	No Hit
ATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTC	5	0.125	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	5	0.125	No Hit
GTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGAT	5	0.125	No Hit
CAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACAC	5	0.125	No Hit
ATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTA	5	0.125	No Hit
GGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACA	5	0.125	No Hit
GAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGA	5	0.125	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	5	0.125	No Hit
TTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAG	5	0.125	No Hit
GACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAA	5	0.125	No Hit
GACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGA	5	0.125	No Hit
AGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGG	5	0.125	No Hit
GATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGG	5	0.125	No Hit
ACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAAT	5	0.125	No Hit
ACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTT	5	0.125	No Hit
GCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTAC	5	0.125	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	5	0.125	No Hit
CGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAG	5	0.125	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.125	0.0	0.0	0.0	0.0
76-77	0.2	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.2875	0.0	0.0	0.0	0.0
82-83	0.3625	0.0	0.0	0.0	0.0
84-85	0.4875	0.0	0.0	0.0	0.0
86-87	0.5375000000000001	0.0	0.0	0.0	0.0
88-89	0.6875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
Read 1871274 spots for SRR24882416.sra
Written 1871274 spots for SRR24882416.sra
Read 1871259 spots for SRR24882416.sra
Written 1871259 spots for SRR24882416.sra
SRR ids: ['SRR24882416.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_q_upizgc
SRR24882416.sra spots: 29940159
blocks: [[1, 1871259], [1871260, 3742518], [3742519, 5613777], [5613778, 7485036], [7485037, 9356295], [9356296, 11227554], [11227555, 13098813], [13098814, 14970072], [14970073, 16841331], [16841332, 18712590], [18712591, 20583849], [20583850, 22455108], [22455109, 24326367], [24326368, 26197626], [26197627, 28068885], [28068886, 29940159]]
SRR24882416 file size 914930
SRR24882416 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24882416 SRR24882416_1.fastq SRR24882416_2.fastq
Input file:	SRR24882416_1.fastq
Paired file:	SRR24882416_2.fastq
trimmed:	SRR24882416-trimmed-pair1.fastq, SRR24882416-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 06:08:00 2026 >> started

Fri Jul  3 06:08:48 2026 >> done (47.813s)
29940159 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       2 ( 0.00%) empty read pairs filtered out after trimming by size control
29940157 (100.00%) read pairs available; of these:
  653246 ( 2.18%) trimmed read pairs available after processing
29286911 (97.82%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       1	  0.00%
 66	       9	  0.00%
 67	     611	  0.00%
 68	    4131	  0.01%
 69	    5235	  0.02%
 70	    5747	  0.02%
 71	    6494	  0.02%
 72	    7369	  0.02%
 73	    7748	  0.03%
 74	    8290	  0.03%
 75	    9375	  0.03%
 76	    9916	  0.03%
 77	   10918	  0.04%
 78	   11424	  0.04%
 79	   12093	  0.04%
 80	   12755	  0.04%
 81	   14048	  0.05%
 82	   14576	  0.05%
 83	   15636	  0.05%
 84	   16816	  0.06%
 85	   17869	  0.06%
 86	   18575	  0.06%
 87	   20158	  0.07%
 88	   21795	  0.07%
 89	   21718	  0.07%
 90	   24476	  0.08%
 91	   25646	  0.09%
 92	   26817	  0.09%
 93	   29151	  0.10%
 94	   30546	  0.10%
 95	   31677	  0.11%
 96	   33179	  0.11%
 97	   36270	  0.12%
 98	   37023	  0.12%
 99	   40582	  0.14%
100	   64572	  0.22%
101	29286911	 97.82%
29940157 reads passed initial QC


criterion=sequence-density
sequence-density=0.43
sequence-density-rank=1
fanout-score=2.74
fanout-score-rank=30
prefix-density=0.49
prefix-fanout=2.4
sequence=GATTCTGCCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=26
fanout-score=33.39
fanout-score-rank=1
prefix-density=0.08
prefix-fanout=6.0
sequence=GTGCTGGGGGCCTGCCCACTTCCACCCTCTTCACATCCCCCTCCTTTCCAGAGCGTTGCACATCCTTCTCCTAGTCCATCTGTTCCCGAGCAGGCCATTCCCAGGGGCCTCTCCTCTCACCAGGATCTCTTGTCTCTTCTTCTGGGGCTGAGGGTGCTCAGCTCTCCACTCCTAGTGCCCGCCATCCTCGCTCCCTTCCCTGTGGGGACTCAGGACACCTAGGCATCCTGGCCTCGGGCTCCCTCCTCCCATAGCTCCCACCTCTGGGCAAACTACAAAGCAGCCACTGCCAGCAGCTCTCCTCCATTGTCCTAGCCAGGAAGTGCCTAGCCTGAGACAGACACAGTGGAGTCTGAG


criterion=sequence-density
sequence-density=0.46
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=36
prefix-density=0.45
prefix-fanout=2.0
sequence=TTGGGCAGAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=93.98
fanout-score-rank=1
prefix-density=0.15
prefix-fanout=3.8
sequence=GGGGAAACTTGTGAATGTAACTTGCCTGTGCCGTGTGACGGTCACGTGGGCCTGGTCTTTTGTGTGTGAGGCCCTTGACCGTGTGGCCTCTGCCTGGCTGTTTGGGGTCCTGCACGGCTTTCCCACCACCTGTAGCTCTTGTTGACCTGCCTGTTCACCTCATGAGTGAAGCGTCTGCCTGGCAGTGGGCCATGAACTGAGGGGTCTCTGTGTAGAGTAGAAGCTTCCTGTGCCTCCGGTTGCCAGGAGACAGCCTGTGCAGTTAAATGGAC
SRR24882416 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 06:09:27
                             Started mapping on |	Jul 03 06:09:27
                                    Finished on |	Jul 03 06:11:58
       Mapping speed, Million of reads per hour |	713.81

                          Number of input reads |	29940157
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	23458602
                        Uniquely mapped reads % |	78.35%
                          Average mapped length |	200.66
                       Number of splices: Total |	12056721
            Number of splices: Annotated (sjdb) |	11969563
                       Number of splices: GT/AG |	11947390
                       Number of splices: GC/AG |	86056
                       Number of splices: AT/AC |	8532
               Number of splices: Non-canonical |	14743
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.30
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.50
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6475492
             % of reads mapped to multiple loci |	21.63%
        Number of reads mapped to too many loci |	2118
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6063	6063	6063
N_multimapping	6475492	6475492	6475492
N_noFeature	1298057	21429040	2041556
N_ambiguous	1587829	635504	269991
UnstrandedReadsAssigned:20572716 PositiveStrandReadsAssigned:1394058 NegativeStrandReadsAssigned:21147055
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882416 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882416-trimmed-pair1.fastq
                             SRR24882416-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 29,940,157 reads, 25,766,655 reads pseudoaligned
[quant] estimated average fragment length: 193.545
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,356 rounds

 109283 SRR24882416.ke.tsv
  52636 SRR24882416.se.tsv
 161919 total
==> SRR24882416.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	5	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	1	0	0
ENSMUST00000178862.1	14	3	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	5	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	1	0	0

==> SRR24882416.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	4
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882416 completed mapping pipeline successfully
