Starting /dee2/code/volunteer_pipeline.sh SRR24882411
    current disk space = 438578850816
    free memory = 324274628 
SRR24882411 SRAfilesize
c7b795696f5b9554fdea41760c3c993b  SRR24882411.sra
SRR24882411.sra file validated
SRR24882411 is paired end
SRR24882411 is conventional basespace
SRR24882411 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5605	37.0	37.0	37.0	37.0	37.0
2	36.42925	37.0	37.0	37.0	37.0	37.0
3	36.46675	37.0	37.0	37.0	37.0	37.0
4	36.484	37.0	37.0	37.0	37.0	37.0
5	36.6145	37.0	37.0	37.0	37.0	37.0
6	36.6325	37.0	37.0	37.0	37.0	37.0
7	36.5705	37.0	37.0	37.0	37.0	37.0
8	36.545	37.0	37.0	37.0	37.0	37.0
9	36.6115	37.0	37.0	37.0	37.0	37.0
10-11	36.59275	37.0	37.0	37.0	37.0	37.0
12-13	36.6065	37.0	37.0	37.0	37.0	37.0
14-15	36.57825	37.0	37.0	37.0	37.0	37.0
16-17	36.623000000000005	37.0	37.0	37.0	37.0	37.0
18-19	36.56825	37.0	37.0	37.0	37.0	37.0
20-21	36.6125	37.0	37.0	37.0	37.0	37.0
22-23	36.57725	37.0	37.0	37.0	37.0	37.0
24-25	36.55525	37.0	37.0	37.0	37.0	37.0
26-27	36.525999999999996	37.0	37.0	37.0	37.0	37.0
28-29	36.48375	37.0	37.0	37.0	37.0	37.0
30-31	36.48775	37.0	37.0	37.0	37.0	37.0
32-33	36.442	37.0	37.0	37.0	37.0	37.0
34-35	36.503249999999994	37.0	37.0	37.0	37.0	37.0
36-37	36.495999999999995	37.0	37.0	37.0	37.0	37.0
38-39	36.46625	37.0	37.0	37.0	37.0	37.0
40-41	36.45175	37.0	37.0	37.0	37.0	37.0
42-43	36.4715	37.0	37.0	37.0	37.0	37.0
44-45	36.430499999999995	37.0	37.0	37.0	37.0	37.0
46-47	36.48675	37.0	37.0	37.0	37.0	37.0
48-49	36.37125	37.0	37.0	37.0	37.0	37.0
50-51	36.443749999999994	37.0	37.0	37.0	37.0	37.0
52-53	36.4015	37.0	37.0	37.0	37.0	37.0
54-55	36.391	37.0	37.0	37.0	37.0	37.0
56-57	36.389250000000004	37.0	37.0	37.0	37.0	37.0
58-59	36.41075	37.0	37.0	37.0	37.0	37.0
60-61	36.3365	37.0	37.0	37.0	37.0	37.0
62-63	36.3735	37.0	37.0	37.0	37.0	37.0
64-65	36.34125	37.0	37.0	37.0	37.0	37.0
66-67	36.362	37.0	37.0	37.0	37.0	37.0
68-69	36.29474999999999	37.0	37.0	37.0	37.0	37.0
70-71	36.256	37.0	37.0	37.0	37.0	37.0
72-73	36.246	37.0	37.0	37.0	37.0	37.0
74-75	36.27975	37.0	37.0	37.0	37.0	37.0
76-77	36.25725	37.0	37.0	37.0	37.0	37.0
78-79	36.3535	37.0	37.0	37.0	37.0	37.0
80-81	36.18175	37.0	37.0	37.0	37.0	37.0
82-83	36.253	37.0	37.0	37.0	37.0	37.0
84-85	36.23	37.0	37.0	37.0	37.0	37.0
86-87	36.2635	37.0	37.0	37.0	37.0	37.0
88-89	36.18825	37.0	37.0	37.0	37.0	37.0
90-91	36.223749999999995	37.0	37.0	37.0	37.0	37.0
92-93	36.16	37.0	37.0	37.0	37.0	37.0
94-95	36.203500000000005	37.0	37.0	37.0	37.0	37.0
96-97	36.12425	37.0	37.0	37.0	37.0	37.0
98-99	36.044	37.0	37.0	37.0	37.0	37.0
100-101	35.9125	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	3.0
26	4.0
27	4.0
28	5.0
29	12.0
30	27.0
31	26.0
32	38.0
33	49.0
34	83.0
35	260.0
36	2712.0
37	775.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.3	11.325000000000001	11.025	40.35
2	18.704676169042262	17.20430107526882	36.55913978494624	27.53188297074269
3	19.80495123780945	20.505126281570394	30.257564391097773	29.43235808952238
4	25.224999999999998	28.675	22.125	23.974999999999998
5	24.825	29.849999999999998	24.0	21.325
6	20.125	34.175	28.125	17.575
7	17.625	25.5	41.425	15.45
8	21.65	21.875	32.75	23.724999999999998
9	20.625	23.549999999999997	34.050000000000004	21.775
10-11	22.425	32.7875	24.7	20.0875
12-13	22.3	26.5375	28.7375	22.425
14-15	21.212500000000002	29.625	28.0875	21.075
16-17	21.7	28.225	26.625	23.45
18-19	23.849999999999998	29.175	26.6625	20.3125
20-21	22.35	27.712500000000002	27.250000000000004	22.6875
22-23	22.3625	28.199999999999996	26.8375	22.6
24-25	23.9375	27.700000000000003	27.0125	21.349999999999998
26-27	21.9375	28.5875	28.962500000000002	20.5125
28-29	21.087500000000002	28.875	28.9875	21.05
30-31	20.775	28.65	27.3375	23.2375
32-33	21.0	26.85	29.45	22.7
34-35	20.05	28.6375	27.925	23.3875
36-37	21.8	28.3875	28.4125	21.4
38-39	21.525	29.012500000000003	29.025000000000002	20.4375
40-41	20.45	29.95	28.1125	21.4875
42-43	22.05	30.5125	26.575	20.8625
44-45	19.925	31.162499999999998	26.5625	22.35
46-47	24.349999999999998	30.025000000000002	25.124999999999996	20.5
48-49	22.025	32.3875	25.4625	20.125
50-51	22.2625	28.325	26.337500000000002	23.075000000000003
52-53	21.975	26.25	28.725	23.05
54-55	20.65	28.249999999999996	27.200000000000003	23.9
56-57	20.275000000000002	28.475	28.65	22.6
58-59	22.3625	27.1125	27.287499999999998	23.2375
60-61	20.3125	30.349999999999998	27.025	22.3125
62-63	23.7125	27.575	27.1375	21.575
64-65	22.8625	27.2625	27.35	22.525000000000002
66-67	23.400000000000002	27.425	28.212500000000002	20.962500000000002
68-69	22.5	27.8125	27.8875	21.8
70-71	23.65	27.525	27.35	21.475
72-73	21.025	30.012499999999996	27.3375	21.625
74-75	22.6	28.5875	28.237499999999997	20.575
76-77	24.55	27.0125	27.187499999999996	21.25
78-79	24.637500000000003	27.2625	27.5125	20.5875
80-81	24.462500000000002	27.6	27.287499999999998	20.65
82-83	23.8125	29.462500000000002	27.237499999999997	19.4875
84-85	20.4875	30.6875	26.6	22.225
86-87	23.225	28.499999999999996	26.8	21.475
88-89	25.137500000000003	28.15	27.85	18.862499999999997
90-91	23.4625	27.05	29.049999999999997	20.4375
92-93	22.7125	28.237499999999997	27.037499999999998	22.0125
94-95	21.3125	29.975	26.625	22.0875
96-97	21.6125	28.875	24.875	24.637500000000003
98-99	21.9	29.799999999999997	27.750000000000004	20.549999999999997
100-101	22.1	28.825	28.1125	20.962500000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	1.5
20	2.0
21	3.0
22	3.0
23	2.5
24	3.5
25	13.0
26	22.5
27	13.5
28	10.5
29	17.5
30	37.0
31	63.0
32	105.0
33	123.5
34	99.0
35	119.5
36	128.0
37	116.0
38	96.0
39	86.0
40	120.0
41	187.5
42	252.5
43	215.0
44	159.5
45	163.5
46	218.5
47	256.5
48	199.0
49	136.5
50	120.0
51	108.0
52	95.0
53	80.0
54	62.5
55	64.5
56	64.5
57	48.0
58	60.0
59	88.5
60	73.5
61	41.0
62	24.5
63	12.5
64	8.0
65	4.0
66	1.5
67	9.5
68	10.0
69	7.0
70	5.5
71	2.0
72	3.5
73	5.5
74	6.5
75	4.5
76	4.0
77	3.5
78	3.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.05
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.79927338782925	38.975
2	16.439600363306088	18.099999999999998
3	4.4959128065395095	7.425
4	2.17983651226158	4.8
5	1.1353315168029063	3.125
6	1.1807447774750226	3.9
7	0.7266121707538601	2.8000000000000003
8	0.49954586739327883	2.1999999999999997
9	0.5903723887375113	2.9250000000000003
>10	1.952770208900999	15.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	42	1.05	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	34	0.8500000000000001	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	25	0.625	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	22	0.5499999999999999	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	21	0.525	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	20	0.5	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	20	0.5	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	20	0.5	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	18	0.44999999999999996	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	18	0.44999999999999996	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	17	0.42500000000000004	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	16	0.4	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	15	0.375	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	15	0.375	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	15	0.375	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	14	0.35000000000000003	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	14	0.35000000000000003	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	13	0.325	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	13	0.325	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	13	0.325	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	12	0.3	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	12	0.3	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	12	0.3	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	12	0.3	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	11	0.27499999999999997	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	11	0.27499999999999997	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	11	0.27499999999999997	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	11	0.27499999999999997	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	11	0.27499999999999997	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	11	0.27499999999999997	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	11	0.27499999999999997	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	10	0.25	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	10	0.25	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	10	0.25	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	10	0.25	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	10	0.25	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	10	0.25	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	10	0.25	No Hit
CTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAG	10	0.25	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	10	0.25	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	10	0.25	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	10	0.25	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	10	0.25	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	9	0.22499999999999998	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	9	0.22499999999999998	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	9	0.22499999999999998	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	9	0.22499999999999998	No Hit
GGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGC	9	0.22499999999999998	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	9	0.22499999999999998	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	9	0.22499999999999998	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	9	0.22499999999999998	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	9	0.22499999999999998	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	9	0.22499999999999998	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	9	0.22499999999999998	No Hit
GGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTG	9	0.22499999999999998	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	9	0.22499999999999998	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	8	0.2	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	8	0.2	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	8	0.2	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	8	0.2	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	8	0.2	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	8	0.2	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	8	0.2	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	8	0.2	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	8	0.2	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	8	0.2	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	8	0.2	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	7	0.17500000000000002	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	7	0.17500000000000002	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	7	0.17500000000000002	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	7	0.17500000000000002	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	7	0.17500000000000002	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	7	0.17500000000000002	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	7	0.17500000000000002	No Hit
CTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAGAAA	7	0.17500000000000002	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAAC	7	0.17500000000000002	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	7	0.17500000000000002	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	7	0.17500000000000002	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	7	0.17500000000000002	No Hit
GTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACC	7	0.17500000000000002	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	7	0.17500000000000002	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	7	0.17500000000000002	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	7	0.17500000000000002	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	6	0.15	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	6	0.15	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	6	0.15	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	6	0.15	No Hit
CCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTA	6	0.15	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	6	0.15	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	6	0.15	No Hit
GCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGA	6	0.15	No Hit
AGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACATC	6	0.15	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	6	0.15	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	6	0.15	No Hit
CACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTT	6	0.15	No Hit
CAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGAT	6	0.15	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	6	0.15	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	6	0.15	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	6	0.15	No Hit
TAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACA	6	0.15	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	6	0.15	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	6	0.15	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	6	0.15	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	6	0.15	No Hit
CAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGAT	6	0.15	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	6	0.15	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	6	0.15	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	6	0.15	No Hit
GTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAAC	6	0.15	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	5	0.125	No Hit
GGCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTA	5	0.125	No Hit
GCTCCCTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATT	5	0.125	No Hit
GCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAG	5	0.125	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	5	0.125	No Hit
CCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAAT	5	0.125	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	5	0.125	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	5	0.125	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	5	0.125	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	5	0.125	No Hit
GTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTGGCATTTCTT	5	0.125	No Hit
GTGGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	5	0.125	No Hit
CGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAAC	5	0.125	No Hit
GCCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	5	0.125	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	5	0.125	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	5	0.125	No Hit
ATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTC	5	0.125	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	5	0.125	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	5	0.125	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	5	0.125	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	5	0.125	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	5	0.125	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	5	0.125	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	5	0.125	No Hit
GAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.037500000000000006	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.0625	0.0	0.0	0.0	0.0
76-77	0.0875	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.16249999999999998	0.0	0.0	0.0	0.0
82-83	0.21250000000000002	0.0	0.0	0.0	0.0
84-85	0.3625	0.0	0.0	0.0	0.0
86-87	0.525	0.0	0.0	0.0	0.0
88-89	0.5874999999999999	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882411 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882411_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.54325	37.0	37.0	37.0	37.0	37.0
2	36.375	37.0	37.0	37.0	37.0	37.0
3	36.4885	37.0	37.0	37.0	37.0	37.0
4	36.47	37.0	37.0	37.0	37.0	37.0
5	36.5555	37.0	37.0	37.0	37.0	37.0
6	36.4725	37.0	37.0	37.0	37.0	37.0
7	36.4955	37.0	37.0	37.0	37.0	37.0
8	36.5715	37.0	37.0	37.0	37.0	37.0
9	36.527	37.0	37.0	37.0	37.0	37.0
10-11	36.438	37.0	37.0	37.0	37.0	37.0
12-13	36.465	37.0	37.0	37.0	37.0	37.0
14-15	36.448750000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.50025	37.0	37.0	37.0	37.0	37.0
18-19	36.4855	37.0	37.0	37.0	37.0	37.0
20-21	36.44425	37.0	37.0	37.0	37.0	37.0
22-23	36.398250000000004	37.0	37.0	37.0	37.0	37.0
24-25	36.372749999999996	37.0	37.0	37.0	37.0	37.0
26-27	36.39975	37.0	37.0	37.0	37.0	37.0
28-29	36.3805	37.0	37.0	37.0	37.0	37.0
30-31	36.35825	37.0	37.0	37.0	37.0	37.0
32-33	36.3995	37.0	37.0	37.0	37.0	37.0
34-35	36.39675	37.0	37.0	37.0	37.0	37.0
36-37	36.3675	37.0	37.0	37.0	37.0	37.0
38-39	36.298	37.0	37.0	37.0	37.0	37.0
40-41	36.301249999999996	37.0	37.0	37.0	37.0	37.0
42-43	36.2965	37.0	37.0	37.0	37.0	37.0
44-45	36.22425	37.0	37.0	37.0	37.0	37.0
46-47	36.2255	37.0	37.0	37.0	37.0	37.0
48-49	36.18175	37.0	37.0	37.0	37.0	37.0
50-51	36.229	37.0	37.0	37.0	37.0	37.0
52-53	36.2145	37.0	37.0	37.0	37.0	37.0
54-55	36.216499999999996	37.0	37.0	37.0	37.0	37.0
56-57	36.215	37.0	37.0	37.0	37.0	37.0
58-59	36.294250000000005	37.0	37.0	37.0	37.0	37.0
60-61	36.13425	37.0	37.0	37.0	37.0	37.0
62-63	36.10325	37.0	37.0	37.0	37.0	37.0
64-65	36.159	37.0	37.0	37.0	37.0	37.0
66-67	36.101749999999996	37.0	37.0	37.0	37.0	37.0
68-69	36.185	37.0	37.0	37.0	37.0	37.0
70-71	36.03874999999999	37.0	37.0	37.0	37.0	37.0
72-73	36.04275	37.0	37.0	37.0	37.0	37.0
74-75	36.0745	37.0	37.0	37.0	37.0	37.0
76-77	35.973749999999995	37.0	37.0	37.0	37.0	37.0
78-79	35.9865	37.0	37.0	37.0	37.0	37.0
80-81	35.911	37.0	37.0	37.0	37.0	37.0
82-83	35.8835	37.0	37.0	37.0	37.0	37.0
84-85	35.86525	37.0	37.0	37.0	37.0	37.0
86-87	35.8475	37.0	37.0	37.0	37.0	37.0
88-89	35.91974999999999	37.0	37.0	37.0	37.0	37.0
90-91	35.79325	37.0	37.0	37.0	37.0	37.0
92-93	35.85625	37.0	37.0	37.0	37.0	37.0
94-95	35.821	37.0	37.0	37.0	37.0	37.0
96-97	35.831999999999994	37.0	37.0	37.0	37.0	37.0
98-99	35.86625	37.0	37.0	37.0	37.0	37.0
100-101	35.547	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	0.0
22	1.0
23	0.0
24	0.0
25	1.0
26	6.0
27	4.0
28	11.0
29	14.0
30	22.0
31	32.0
32	33.0
33	81.0
34	143.0
35	454.0
36	2734.0
37	462.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.77983487615712	17.7633224918689	13.284963722792096	29.171878909181885
2	19.7	27.125	37.375	15.8
3	19.650000000000002	26.625	33.324999999999996	20.4
4	24.625	33.375	22.525000000000002	19.475
5	23.875	33.35	24.6	18.175
6	22.225	34.050000000000004	23.575	20.150000000000002
7	19.85	20.549999999999997	39.85	19.75
8	21.25	21.25	32.15	25.35
9	22.2	22.900000000000002	31.324999999999996	23.575
10-11	23.125	31.587500000000002	24.025	21.2625
12-13	24.2375	25.275	26.325	24.1625
14-15	22.575	26.05	29.849999999999998	21.525
16-17	22.8625	28.4375	28.425	20.275000000000002
18-19	22.3125	27.474999999999998	28.9	21.3125
20-21	22.075	28.462500000000002	29.95	19.5125
22-23	21.8875	27.437499999999996	29.549999999999997	21.125
24-25	23.200000000000003	28.262500000000003	27.575	20.962500000000002
26-27	22.0125	27.5125	26.974999999999998	23.5
28-29	23.5	27.05	28.7	20.75
30-31	23.1625	26.900000000000002	29.175	20.7625
32-33	22.5625	26.150000000000002	30.4	20.8875
34-35	22.3125	28.275	27.250000000000004	22.162499999999998
36-37	22.375	27.8625	29.525000000000002	20.2375
38-39	21.712500000000002	27.200000000000003	28.449999999999996	22.6375
40-41	20.775	27.6625	29.4	22.162499999999998
42-43	21.637500000000003	28.8625	27.6125	21.8875
44-45	21.675	27.287499999999998	30.65	20.3875
46-47	22.375	26.85	27.9125	22.8625
48-49	21.5625	26.35	31.087500000000002	21.0
50-51	20.7625	28.3125	28.0875	22.8375
52-53	22.287499999999998	27.737499999999997	29.725	20.25
54-55	23.5875	27.6	27.0875	21.725
56-57	20.4	28.025	29.8875	21.6875
58-59	21.0375	28.275	28.3625	22.325
60-61	20.125	28.512500000000003	29.0875	22.275
62-63	21.8125	28.037499999999998	27.325	22.825
64-65	21.3625	26.737499999999997	30.662499999999998	21.2375
66-67	19.875	26.275	30.337500000000002	23.5125
68-69	21.6125	27.9125	28.725	21.75
70-71	20.9125	28.425	28.8625	21.8
72-73	22.0	28.287499999999998	26.5	23.2125
74-75	22.537499999999998	25.974999999999998	29.299999999999997	22.1875
76-77	22.1	25.7875	29.049999999999997	23.0625
78-79	23.962500000000002	25.7625	29.25	21.025
80-81	21.1625	27.1625	28.875	22.8
82-83	20.9375	27.425	29.212500000000002	22.425
84-85	20.1875	26.75	29.775000000000002	23.2875
86-87	20.9	27.4125	28.625	23.0625
88-89	21.837500000000002	27.4125	28.65	22.1
90-91	23.375	26.525	27.1	23.0
92-93	22.037499999999998	28.3625	28.962500000000002	20.6375
94-95	21.3	29.549999999999997	27.800000000000004	21.349999999999998
96-97	22.0125	28.225	27.5625	22.2
98-99	22.4375	28.287499999999998	27.950000000000003	21.325
100-101	22.0875	27.5125	28.849999999999998	21.55
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.5
17	1.0
18	0.5
19	0.5
20	1.0
21	2.0
22	1.5
23	1.0
24	1.5
25	4.5
26	7.0
27	10.0
28	11.0
29	16.0
30	47.5
31	92.0
32	131.0
33	127.0
34	112.0
35	107.5
36	92.5
37	104.5
38	112.0
39	102.0
40	125.5
41	201.5
42	255.5
43	234.5
44	217.5
45	236.5
46	227.0
47	210.5
48	170.5
49	122.5
50	110.0
51	93.5
52	86.0
53	68.5
54	54.5
55	86.0
56	75.5
57	36.0
58	61.0
59	59.5
60	26.0
61	19.0
62	16.0
63	8.5
64	5.0
65	5.5
66	4.0
67	4.0
68	6.5
69	6.5
70	7.0
71	7.0
72	5.5
73	8.0
74	13.0
75	10.0
76	8.5
77	11.0
78	7.0
79	3.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.425000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.94107221976074	38.9
2	16.349136021267167	18.45
3	5.848471422241914	9.9
4	3.10146211785556	7.000000000000001
5	1.8165706690296854	5.125
6	1.01905183872397	3.45
7	0.5316792202038104	2.1
8	0.6202924235711121	2.8000000000000003
9	0.5316792202038104	2.7
>10	1.2405848471422243	9.575
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	34	0.8500000000000001	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	24	0.6	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	20	0.5	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	18	0.44999999999999996	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	18	0.44999999999999996	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	17	0.42500000000000004	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	15	0.375	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	14	0.35000000000000003	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	13	0.325	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	13	0.325	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	13	0.325	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	12	0.3	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	12	0.3	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	12	0.3	No Hit
AGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTC	11	0.27499999999999997	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	11	0.27499999999999997	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	11	0.27499999999999997	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	11	0.27499999999999997	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	11	0.27499999999999997	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	11	0.27499999999999997	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	11	0.27499999999999997	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	11	0.27499999999999997	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	10	0.25	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	10	0.25	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	10	0.25	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	10	0.25	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	10	0.25	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	10	0.25	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	9	0.22499999999999998	No Hit
GGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGA	9	0.22499999999999998	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	9	0.22499999999999998	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	9	0.22499999999999998	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	9	0.22499999999999998	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	9	0.22499999999999998	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	9	0.22499999999999998	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	9	0.22499999999999998	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	9	0.22499999999999998	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	9	0.22499999999999998	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	9	0.22499999999999998	No Hit
CTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTC	9	0.22499999999999998	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	8	0.2	No Hit
TGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAG	8	0.2	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	8	0.2	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	8	0.2	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	8	0.2	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	8	0.2	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	8	0.2	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	8	0.2	No Hit
TAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTC	8	0.2	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	8	0.2	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	8	0.2	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	8	0.2	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	8	0.2	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	8	0.2	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	7	0.17500000000000002	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	7	0.17500000000000002	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	7	0.17500000000000002	No Hit
ATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCT	7	0.17500000000000002	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	7	0.17500000000000002	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	7	0.17500000000000002	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	7	0.17500000000000002	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	7	0.17500000000000002	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	7	0.17500000000000002	No Hit
TTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTG	7	0.17500000000000002	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	7	0.17500000000000002	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	7	0.17500000000000002	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	6	0.15	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	6	0.15	No Hit
CAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGC	6	0.15	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	6	0.15	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	6	0.15	No Hit
GGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACA	6	0.15	No Hit
ATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCC	6	0.15	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	6	0.15	No Hit
GACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTT	6	0.15	No Hit
CTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGT	6	0.15	No Hit
ATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGT	6	0.15	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	6	0.15	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	6	0.15	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	6	0.15	No Hit
TGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAAT	6	0.15	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	6	0.15	No Hit
GCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAG	6	0.15	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	6	0.15	No Hit
AATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCA	6	0.15	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	6	0.15	No Hit
CTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAG	6	0.15	No Hit
GTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACA	5	0.125	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	5	0.125	No Hit
AGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATT	5	0.125	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	5	0.125	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	5	0.125	No Hit
CTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATT	5	0.125	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	5	0.125	No Hit
GTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAA	5	0.125	No Hit
GTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTT	5	0.125	No Hit
ACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAA	5	0.125	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	5	0.125	No Hit
AGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTT	5	0.125	No Hit
CCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGAT	5	0.125	No Hit
TGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGAC	5	0.125	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	5	0.125	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	5	0.125	No Hit
CTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTC	5	0.125	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	5	0.125	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	5	0.125	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	5	0.125	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	5	0.125	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	5	0.125	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	5	0.125	No Hit
CAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAA	5	0.125	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	5	0.125	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	5	0.125	No Hit
TGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	5	0.125	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	5	0.125	No Hit
GGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGT	5	0.125	No Hit
TGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTGAT	5	0.125	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	5	0.125	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	5	0.125	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	5	0.125	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	5	0.125	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	5	0.125	No Hit
GTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTT	5	0.125	No Hit
ATGAGCCATACTGCATATGTGTTGAGTTGGTCAAGGGCTTAATTCATAAA	5	0.125	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	5	0.125	No Hit
ACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.037500000000000006	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.0625	0.0	0.0	0.0	0.0
76-77	0.0875	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.16249999999999998	0.0	0.0	0.0	0.0
82-83	0.21250000000000002	0.0	0.0	0.0	0.0
84-85	0.3375	0.0	0.0	0.0	0.0
86-87	0.5	0.0	0.0	0.0	0.0
88-89	0.5625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
Read 1992341 spots for SRR24882411.sra
Written 1992341 spots for SRR24882411.sra
Read 1992331 spots for SRR24882411.sra
Written 1992331 spots for SRR24882411.sra
SRR ids: ['SRR24882411.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__k33epzp
SRR24882411.sra spots: 31877306
blocks: [[1, 1992331], [1992332, 3984662], [3984663, 5976993], [5976994, 7969324], [7969325, 9961655], [9961656, 11953986], [11953987, 13946317], [13946318, 15938648], [15938649, 17930979], [17930980, 19923310], [19923311, 21915641], [21915642, 23907972], [23907973, 25900303], [25900304, 27892634], [27892635, 29884965], [29884966, 31877306]]
SRR24882411 file size 1067461
SRR24882411 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24882411 SRR24882411_1.fastq SRR24882411_2.fastq
Input file:	SRR24882411_1.fastq
Paired file:	SRR24882411_2.fastq
trimmed:	SRR24882411-trimmed-pair1.fastq, SRR24882411-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 05:46:33 2026 >> started

Fri Jul  3 05:47:46 2026 >> done (73.182s)
31877306 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
31877306 (100.00%) read pairs available; of these:
  823226 ( 2.58%) trimmed read pairs available after processing
31054080 (97.42%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       2	  0.00%
 66	      14	  0.00%
 67	     555	  0.00%
 68	    4739	  0.01%
 69	    6033	  0.02%
 70	    6657	  0.02%
 71	    7697	  0.02%
 72	    8470	  0.03%
 73	    9104	  0.03%
 74	    9997	  0.03%
 75	   11148	  0.03%
 76	   11696	  0.04%
 77	   13105	  0.04%
 78	   13824	  0.04%
 79	   14781	  0.05%
 80	   16261	  0.05%
 81	   17541	  0.06%
 82	   18239	  0.06%
 83	   19805	  0.06%
 84	   21572	  0.07%
 85	   22853	  0.07%
 86	   23498	  0.07%
 87	   25539	  0.08%
 88	   28349	  0.09%
 89	   28536	  0.09%
 90	   31282	  0.10%
 91	   33576	  0.11%
 92	   34806	  0.11%
 93	   38096	  0.12%
 94	   39991	  0.13%
 95	   41340	  0.13%
 96	   43410	  0.14%
 97	   47780	  0.15%
 98	   48136	  0.15%
 99	   53039	  0.17%
100	   71755	  0.23%
101	31054080	 97.42%
31877306 reads passed initial QC


criterion=sequence-density
sequence-density=0.46
sequence-density-rank=1
fanout-score=2.87
fanout-score-rank=25
prefix-density=0.53
prefix-fanout=2.5
sequence=GATTCTGCCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=33.46
fanout-score-rank=1
prefix-density=0.08
prefix-fanout=2.3
sequence=TGGGGTAGGTCTTTATTTGGGGGGGTTCCAGCAGGCCCAGCCCCCTCCAACAGTAAGTCAGAGCATGCACTGGCCTTCCCTCCTGACCAAGGACAAGGCCCCATTCCCCCACTCCACAGGGCTGTAGTGACAACACTTCAGAAGGGAAGGAAAAATAAAAACATAAACCCCTAAGGAATGGATTGTCTGCATAGAAACCTCATTTAGTTTAAATTAGAAACAGGGGCTGCAAGTGGCTTGCTTCTTCCAGGTGGGTTTTCTGTAAGGTAAGGCTCTGTCTTCACTTGGGTTGGGATAGGTTGGGTTGGGTAGGTTCAAATCGGGACAGGATGGGATACTGGAGTGGAGGTAGACGCTGGCCTGGGGTCAGGCACCCTGGGCACCTCCCCTCCCCCGCCTGCCCCACCACTCAGGTGGCGTTCCTCTGGCGTACATTCTTGTCCTTGTCATTCATGTGAGTTCCACTGCCCTTCAGTGGGGCGGCGCCAGGGTCATCCTCGTCCAGGGTCTG


criterion=sequence-density
sequence-density=0.49
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=36
prefix-density=0.49
prefix-fanout=1.9
sequence=TTGGGCAGAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=82.73
fanout-score-rank=1
prefix-density=0.14
prefix-fanout=3.7
sequence=GGGGAAACTTGTGAATGTAACTTGCCTGTGCCGTGTGACGGTCACGTGGGCCTGGTCTTTTGTGTGTGAGGCCCTTGACCGTGTGGCCTCTGCCTGGCTGTTTGGGGTCCTGCACGGCTTTCCCACCACCTGTAGCTCTTGTTGACCTGCCTGTTCACCTCATGAGTGAAGCGTCTGCCTGGCAGTGGGCCATGAACTGAGGGGTCTCTGTGTAGAGTAGAAGCTTCCTGTGCCTCCGGTTGCCAGGAGACAGCCTGTGCAGTTAAATGGACCTAGAT
SRR24882411 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 05:48:27
                             Started mapping on |	Jul 03 05:48:27
                                    Finished on |	Jul 03 05:51:20
       Mapping speed, Million of reads per hour |	663.34

                          Number of input reads |	31877306
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	25590336
                        Uniquely mapped reads % |	80.28%
                          Average mapped length |	200.61
                       Number of splices: Total |	12446532
            Number of splices: Annotated (sjdb) |	12340004
                       Number of splices: GT/AG |	12327002
                       Number of splices: GC/AG |	93242
                       Number of splices: AT/AC |	8659
               Number of splices: Non-canonical |	17629
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.91
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.55
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6280421
             % of reads mapped to multiple loci |	19.70%
        Number of reads mapped to too many loci |	2556
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6549	6549	6549
N_multimapping	6280421	6280421	6280421
N_noFeature	2630497	23614653	3370149
N_ambiguous	1573833	566116	296864
UnstrandedReadsAssigned:21386006 PositiveStrandReadsAssigned:1409567 NegativeStrandReadsAssigned:21923323
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882411 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882411-trimmed-pair1.fastq
                             SRR24882411-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,877,306 reads, 26,342,600 reads pseudoaligned
[quant] estimated average fragment length: 188.483
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,737 rounds

 109283 SRR24882411.ke.tsv
  52636 SRR24882411.se.tsv
 161919 total
==> SRR24882411.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	14	0	0
ENSMUST00000196221.1	9	7	0	0
ENSMUST00000179664.1	11	9	0	0
ENSMUST00000178537.1	12	10	0	0
ENSMUST00000178862.1	14	12	0	0
ENSMUST00000179520.1	11	9	0	0
ENSMUST00000179883.1	16	14	0	0
ENSMUST00000195858.1	10	8	0	0
ENSMUST00000179932.1	12	10	0	0

==> SRR24882411.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882411 completed mapping pipeline successfully
