Starting /dee2/code/volunteer_pipeline.sh SRR24882420
    current disk space = 1385002048
    free memory = 196310496 
SRR24882420 SRAfilesize
ea4c561e0edbb936c7edacddf18ef144  SRR24882420.sra
SRR24882420.sra file validated
SRR24882420 is paired end
SRR24882420 is conventional basespace
SRR24882420 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882420_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.61525	37.0	37.0	37.0	37.0	37.0
2	36.53	37.0	37.0	37.0	37.0	37.0
3	36.55775	37.0	37.0	37.0	37.0	37.0
4	36.6625	37.0	37.0	37.0	37.0	37.0
5	36.6905	37.0	37.0	37.0	37.0	37.0
6	36.612	37.0	37.0	37.0	37.0	37.0
7	36.63	37.0	37.0	37.0	37.0	37.0
8	36.6045	37.0	37.0	37.0	37.0	37.0
9	36.669	37.0	37.0	37.0	37.0	37.0
10-11	36.61125	37.0	37.0	37.0	37.0	37.0
12-13	36.64375	37.0	37.0	37.0	37.0	37.0
14-15	36.616	37.0	37.0	37.0	37.0	37.0
16-17	36.658500000000004	37.0	37.0	37.0	37.0	37.0
18-19	36.615750000000006	37.0	37.0	37.0	37.0	37.0
20-21	36.616	37.0	37.0	37.0	37.0	37.0
22-23	36.602999999999994	37.0	37.0	37.0	37.0	37.0
24-25	36.613	37.0	37.0	37.0	37.0	37.0
26-27	36.594	37.0	37.0	37.0	37.0	37.0
28-29	36.53475	37.0	37.0	37.0	37.0	37.0
30-31	36.591750000000005	37.0	37.0	37.0	37.0	37.0
32-33	36.58225	37.0	37.0	37.0	37.0	37.0
34-35	36.55475	37.0	37.0	37.0	37.0	37.0
36-37	36.546499999999995	37.0	37.0	37.0	37.0	37.0
38-39	36.51775	37.0	37.0	37.0	37.0	37.0
40-41	36.45925	37.0	37.0	37.0	37.0	37.0
42-43	36.4465	37.0	37.0	37.0	37.0	37.0
44-45	36.566500000000005	37.0	37.0	37.0	37.0	37.0
46-47	36.5505	37.0	37.0	37.0	37.0	37.0
48-49	36.47375	37.0	37.0	37.0	37.0	37.0
50-51	36.4615	37.0	37.0	37.0	37.0	37.0
52-53	36.477999999999994	37.0	37.0	37.0	37.0	37.0
54-55	36.498999999999995	37.0	37.0	37.0	37.0	37.0
56-57	36.40875	37.0	37.0	37.0	37.0	37.0
58-59	36.4815	37.0	37.0	37.0	37.0	37.0
60-61	36.488749999999996	37.0	37.0	37.0	37.0	37.0
62-63	36.47625	37.0	37.0	37.0	37.0	37.0
64-65	36.412	37.0	37.0	37.0	37.0	37.0
66-67	36.44975	37.0	37.0	37.0	37.0	37.0
68-69	36.363	37.0	37.0	37.0	37.0	37.0
70-71	36.396	37.0	37.0	37.0	37.0	37.0
72-73	36.315250000000006	37.0	37.0	37.0	37.0	37.0
74-75	36.3525	37.0	37.0	37.0	37.0	37.0
76-77	36.38975	37.0	37.0	37.0	37.0	37.0
78-79	36.3345	37.0	37.0	37.0	37.0	37.0
80-81	36.30475	37.0	37.0	37.0	37.0	37.0
82-83	36.3975	37.0	37.0	37.0	37.0	37.0
84-85	36.2195	37.0	37.0	37.0	37.0	37.0
86-87	36.268249999999995	37.0	37.0	37.0	37.0	37.0
88-89	36.233999999999995	37.0	37.0	37.0	37.0	37.0
90-91	36.2785	37.0	37.0	37.0	37.0	37.0
92-93	36.26375	37.0	37.0	37.0	37.0	37.0
94-95	36.20825	37.0	37.0	37.0	37.0	37.0
96-97	36.13225	37.0	37.0	37.0	37.0	37.0
98-99	36.1345	37.0	37.0	37.0	37.0	37.0
100-101	36.00675	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	3.0
27	3.0
28	7.0
29	15.0
30	15.0
31	27.0
32	32.0
33	35.0
34	75.0
35	252.0
36	2670.0
37	866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.40960240060015	8.052013003250813	13.353338334583645	40.1850462615654
2	17.549999999999997	13.3	37.9	31.25
3	19.864898674005506	17.338003502626968	31.898924193144857	30.89817363022267
4	26.825	24.75	23.375	25.05
5	26.674999999999997	25.324999999999996	26.325	21.675
6	21.575	29.5	30.85	18.075
7	17.0	21.4	45.525	16.075
8	21.675	20.175	34.725	23.425
9	21.425	21.6	35.449999999999996	21.525
10-11	22.2625	29.2875	28.1625	20.2875
12-13	22.525000000000002	23.8875	30.0375	23.549999999999997
14-15	19.7375	28.075	30.862499999999997	21.325
16-17	20.875	26.787499999999998	28.199999999999996	24.1375
18-19	23.0625	27.3375	26.687499999999996	22.912499999999998
20-21	22.425	26.400000000000002	27.6875	23.4875
22-23	22.1375	27.224999999999998	27.175	23.4625
24-25	23.962500000000002	26.637499999999996	26.1125	23.2875
26-27	21.575	27.325	29.062500000000004	22.037499999999998
28-29	21.05	27.075	28.999999999999996	22.875
30-31	20.549999999999997	25.4875	29.575000000000003	24.3875
32-33	21.525	25.3	28.825	24.349999999999998
34-35	20.0875	26.8125	28.825	24.275
36-37	22.537499999999998	27.325	28.287499999999998	21.85
38-39	20.175	27.800000000000004	30.012499999999996	22.0125
40-41	21.25	27.175	30.625000000000004	20.95
42-43	21.725	29.1875	28.0625	21.025
44-45	19.9125	29.3375	27.9375	22.8125
46-47	21.85	28.675	26.3625	23.1125
48-49	21.4375	27.650000000000002	28.9375	21.975
50-51	22.875	24.85	29.325000000000003	22.95
52-53	21.25	25.162499999999998	29.25	24.337500000000002
54-55	22.05	26.2625	28.3125	23.375
56-57	19.0625	27.575	29.825000000000003	23.5375
58-59	21.512500000000003	25.912499999999998	28.349999999999998	24.224999999999998
60-61	20.0875	27.800000000000004	28.549999999999997	23.5625
62-63	22.7375	25.650000000000002	28.799999999999997	22.8125
64-65	24.6125	24.775	28.962500000000002	21.65
66-67	23.35	26.174999999999997	28.6875	21.7875
68-69	21.0375	26.575	28.625	23.7625
70-71	21.712500000000002	28.175	26.5875	23.525
72-73	20.65	29.262500000000003	27.400000000000002	22.6875
74-75	21.125	27.3125	29.562500000000004	22.0
76-77	23.025000000000002	27.474999999999998	27.3	22.2
78-79	22.662499999999998	26.424999999999997	28.9375	21.975
80-81	23.849999999999998	24.5375	30.2	21.4125
82-83	23.150000000000002	27.500000000000004	28.012500000000003	21.337500000000002
84-85	20.3625	29.5	24.9875	25.15
86-87	22.7375	28.225	27.537499999999998	21.5
88-89	23.8875	26.5	28.349999999999998	21.2625
90-91	20.599999999999998	27.750000000000004	28.1375	23.5125
92-93	22.1375	27.575	28.050000000000004	22.237499999999997
94-95	22.075	27.3625	27.487499999999997	23.075000000000003
96-97	21.15	27.987499999999997	25.525	25.337500000000002
98-99	19.6125	28.487499999999997	29.4875	22.412499999999998
100-101	21.55	26.237500000000004	28.449999999999996	23.7625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5
22	2.5
23	1.5
24	2.5
25	11.0
26	12.5
27	8.0
28	7.5
29	16.5
30	52.0
31	73.5
32	103.5
33	128.0
34	114.0
35	132.0
36	113.0
37	73.0
38	70.0
39	59.0
40	76.5
41	134.0
42	194.5
43	161.0
44	103.5
45	140.5
46	227.0
47	292.0
48	262.0
49	208.0
50	184.0
51	144.5
52	129.0
53	110.0
54	94.5
55	94.0
56	77.0
57	68.0
58	67.5
59	86.5
60	68.0
61	26.5
62	15.0
63	9.0
64	2.0
65	1.0
66	2.5
67	6.5
68	7.0
69	5.0
70	4.5
71	1.5
72	1.0
73	1.5
74	3.5
75	3.0
76	2.0
77	3.0
78	1.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.075
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.620652755484215	28.325
2	18.45906902086677	17.25
3	8.453718566078116	11.85
4	3.9593365436062067	7.3999999999999995
5	2.1936864633493847	5.125
6	1.3911182450508293	3.9
7	1.0165864098448367	3.325
8	0.6955591225254146	2.6
9	0.7490636704119851	3.15
>10	2.4612092027822365	17.075000000000003
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	39	0.975	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	30	0.75	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	30	0.75	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	22	0.5499999999999999	No Hit
GTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCGGAAGGTCTTGTTTGGG	21	0.525	No Hit
GCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTTT	20	0.5	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	20	0.5	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	20	0.5	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	19	0.475	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	19	0.475	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	19	0.475	No Hit
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	18	0.44999999999999996	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	17	0.42500000000000004	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	16	0.4	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	15	0.375	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	14	0.35000000000000003	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	14	0.35000000000000003	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	13	0.325	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	13	0.325	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	13	0.325	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	13	0.325	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	12	0.3	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	12	0.3	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	12	0.3	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	12	0.3	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	12	0.3	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	12	0.3	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	12	0.3	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	12	0.3	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	12	0.3	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	11	0.27499999999999997	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	11	0.27499999999999997	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	11	0.27499999999999997	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	11	0.27499999999999997	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	11	0.27499999999999997	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	11	0.27499999999999997	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	11	0.27499999999999997	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	11	0.27499999999999997	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	11	0.27499999999999997	No Hit
GTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAAC	11	0.27499999999999997	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	10	0.25	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	10	0.25	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	10	0.25	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	10	0.25	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	10	0.25	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	10	0.25	No Hit
AGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATC	9	0.22499999999999998	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	9	0.22499999999999998	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	9	0.22499999999999998	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	9	0.22499999999999998	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	9	0.22499999999999998	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	9	0.22499999999999998	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	9	0.22499999999999998	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	9	0.22499999999999998	No Hit
CAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGAT	9	0.22499999999999998	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	9	0.22499999999999998	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	9	0.22499999999999998	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	9	0.22499999999999998	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	9	0.22499999999999998	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	9	0.22499999999999998	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	8	0.2	No Hit
TTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAA	8	0.2	No Hit
GTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGC	8	0.2	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	8	0.2	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	8	0.2	No Hit
GTCTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTT	8	0.2	No Hit
CCTTCTTTTGCAGAACTTTCCACACCCATCACCAAAATATTTGCTGCATC	8	0.2	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	8	0.2	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	8	0.2	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	8	0.2	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	8	0.2	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	8	0.2	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	8	0.2	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	7	0.17500000000000002	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	7	0.17500000000000002	No Hit
GTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCCACTTC	7	0.17500000000000002	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	7	0.17500000000000002	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	7	0.17500000000000002	No Hit
CATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTT	7	0.17500000000000002	No Hit
CTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTTTT	7	0.17500000000000002	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	7	0.17500000000000002	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	7	0.17500000000000002	No Hit
CGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGG	7	0.17500000000000002	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	7	0.17500000000000002	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	7	0.17500000000000002	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	7	0.17500000000000002	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	7	0.17500000000000002	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	7	0.17500000000000002	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	7	0.17500000000000002	No Hit
GGCTCCTGTAGGTCGTCCTCTTCTGTCTGGGCCTTGTTCATTTCTTCTTT	7	0.17500000000000002	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	7	0.17500000000000002	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	7	0.17500000000000002	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	6	0.15	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	6	0.15	No Hit
GCCTCTTTACTGCTTTTATAAACCACTTAGAACACAAGAGATTTGGTTTG	6	0.15	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	6	0.15	No Hit
CTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAGCTCAAACATTAC	6	0.15	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	6	0.15	No Hit
CCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGC	6	0.15	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	6	0.15	No Hit
GCCATTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAG	6	0.15	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	6	0.15	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	6	0.15	No Hit
CCTCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCC	6	0.15	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	6	0.15	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	6	0.15	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	6	0.15	No Hit
CTCTAGAACTTGCATCACGTGCTTCATAGTTTTTTCTTTTACATTCCAGA	6	0.15	No Hit
CTCACTTCAATCTGCATATGAATTCGGTCCAGCCTAGAAGAGTACAATCC	6	0.15	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	6	0.15	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	6	0.15	No Hit
TTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCAT	6	0.15	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	6	0.15	No Hit
CCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAGTTGAGTTCT	6	0.15	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	6	0.15	No Hit
GTCAGGTTTTATCCAAAAATGAAGATTGCCGTCACATTATTACTCTTTTG	6	0.15	No Hit
CAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGT	6	0.15	No Hit
CCCAGCTCAAACATTACCTCAATGGTGATGAGGATGACGATCATCCACTC	5	0.125	No Hit
CCTGGATGGCTGCCGGGTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCG	5	0.125	No Hit
GGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCT	5	0.125	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	5	0.125	No Hit
CTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGATCCATTCT	5	0.125	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	5	0.125	No Hit
TCTGGAATGGACTGAATAGACTCAATAAATTTGTCCAGGGTTGCTTCCCA	5	0.125	No Hit
AAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGA	5	0.125	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	5	0.125	No Hit
GTCCCTCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGA	5	0.125	No Hit
CTCCGGGCTAATACTCTGTTCTTCATTACTCTCAGTTTCCTGACATCTGG	5	0.125	No Hit
CTTGTCATAAAGCTCTTCCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGG	5	0.125	No Hit
GAGAGCCACTTCATAGGGCTGGGTTTCGTGTCTCTCTAGAACTTGCATCA	5	0.125	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	5	0.125	No Hit
GTCGTAATGTTGGGACATTCTGAATGTCTCAAAATGGAGTTTTTATTTCT	5	0.125	No Hit
CCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTG	5	0.125	No Hit
GTGCGTTTCCCTGTGTAGCCAGGGCTGCTCTGGACATCAGGATCTGCCTG	5	0.125	No Hit
GGGGCTTCTCAAAGCTTCGCTATGGCTGCACACCCATTATATACTGCATA	5	0.125	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	5	0.125	No Hit
GTCATTTGTGTTTTATTTTGAGACAGTTCTGCTGTGTTATTGTTGGCCTC	5	0.125	No Hit
GCTTAATCTGACACTAAGGAGACTTCTTTCCAACCTGGACTGGATTAGAA	5	0.125	No Hit
GTGCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAGCTCAAACAT	5	0.125	No Hit
TCTGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTT	5	0.125	No Hit
CCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGTTGGAGAACGCAAATT	5	0.125	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	5	0.125	No Hit
CTCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCCA	5	0.125	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	5	0.125	No Hit
CTGGAATGGACTGAATAGACTCAATAAATTTGTCCAGGGTTGCTTCCCAA	5	0.125	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	5	0.125	No Hit
AAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACA	5	0.125	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	5	0.125	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	5	0.125	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	5	0.125	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	5	0.125	No Hit
GTTTTATTTTGAGACAGTTCTGCTGTGTTATTGTTGGCCTCAAACTGGGC	5	0.125	No Hit
GGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTT	5	0.125	No Hit
TCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGGCAATAAG	5	0.125	No Hit
CCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGGTGATCAAGAAATCTGAG	5	0.125	No Hit
GCTCCCATCCCACCTGGCGCCCAGGCAGCATCAACTTTAGCCCTCATTAA	5	0.125	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	5	0.125	No Hit
TTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0625	0.0	0.0	0.0	0.0
72-73	0.1375	0.0	0.0	0.0	0.0
74-75	0.175	0.0	0.0	0.0	0.0
76-77	0.35	0.0	0.0	0.0	0.0
78-79	0.45	0.0	0.0	0.0	0.0
80-81	0.4625	0.0	0.0	0.0	0.0
82-83	0.6125	0.0	0.0	0.0	0.0
84-85	0.825	0.0	0.0	0.0	0.0
86-87	1.0625	0.0	0.0	0.0	0.0
88-89	1.1875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882420 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882420_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.44975	37.0	37.0	37.0	37.0	37.0
2	36.3885	37.0	37.0	37.0	37.0	37.0
3	36.454	37.0	37.0	37.0	37.0	37.0
4	36.554	37.0	37.0	37.0	37.0	37.0
5	36.471	37.0	37.0	37.0	37.0	37.0
6	36.3815	37.0	37.0	37.0	37.0	37.0
7	36.49	37.0	37.0	37.0	37.0	37.0
8	36.5445	37.0	37.0	37.0	37.0	37.0
9	36.551	37.0	37.0	37.0	37.0	37.0
10-11	36.516999999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.5215	37.0	37.0	37.0	37.0	37.0
14-15	36.433499999999995	37.0	37.0	37.0	37.0	37.0
16-17	36.52125	37.0	37.0	37.0	37.0	37.0
18-19	36.443	37.0	37.0	37.0	37.0	37.0
20-21	36.393	37.0	37.0	37.0	37.0	37.0
22-23	36.425250000000005	37.0	37.0	37.0	37.0	37.0
24-25	36.317499999999995	37.0	37.0	37.0	37.0	37.0
26-27	36.349500000000006	37.0	37.0	37.0	37.0	37.0
28-29	36.36775	37.0	37.0	37.0	37.0	37.0
30-31	36.39475	37.0	37.0	37.0	37.0	37.0
32-33	36.385999999999996	37.0	37.0	37.0	37.0	37.0
34-35	36.41	37.0	37.0	37.0	37.0	37.0
36-37	36.4025	37.0	37.0	37.0	37.0	37.0
38-39	36.323499999999996	37.0	37.0	37.0	37.0	37.0
40-41	36.4055	37.0	37.0	37.0	37.0	37.0
42-43	36.26775	37.0	37.0	37.0	37.0	37.0
44-45	36.28725	37.0	37.0	37.0	37.0	37.0
46-47	36.338499999999996	37.0	37.0	37.0	37.0	37.0
48-49	36.153999999999996	37.0	37.0	37.0	37.0	37.0
50-51	36.202	37.0	37.0	37.0	37.0	37.0
52-53	36.23675	37.0	37.0	37.0	37.0	37.0
54-55	36.27025	37.0	37.0	37.0	37.0	37.0
56-57	36.191	37.0	37.0	37.0	37.0	37.0
58-59	36.1435	37.0	37.0	37.0	37.0	37.0
60-61	36.141	37.0	37.0	37.0	37.0	37.0
62-63	36.0605	37.0	37.0	37.0	37.0	37.0
64-65	36.1425	37.0	37.0	37.0	37.0	37.0
66-67	36.109	37.0	37.0	37.0	37.0	37.0
68-69	36.0835	37.0	37.0	37.0	37.0	37.0
70-71	36.054	37.0	37.0	37.0	37.0	37.0
72-73	36.063500000000005	37.0	37.0	37.0	37.0	37.0
74-75	36.05075	37.0	37.0	37.0	37.0	37.0
76-77	36.05225	37.0	37.0	37.0	37.0	37.0
78-79	35.986000000000004	37.0	37.0	37.0	37.0	37.0
80-81	35.97875	37.0	37.0	37.0	37.0	37.0
82-83	35.966499999999996	37.0	37.0	37.0	37.0	37.0
84-85	35.98925	37.0	37.0	37.0	37.0	37.0
86-87	35.99025	37.0	37.0	37.0	37.0	37.0
88-89	35.95725	37.0	37.0	37.0	37.0	37.0
90-91	35.82875	37.0	37.0	37.0	37.0	37.0
92-93	35.935	37.0	37.0	37.0	37.0	37.0
94-95	35.857	37.0	37.0	37.0	37.0	37.0
96-97	35.869	37.0	37.0	37.0	37.0	37.0
98-99	35.826750000000004	37.0	37.0	37.0	37.0	37.0
100-101	35.54625	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	3.0
25	5.0
26	5.0
27	5.0
28	5.0
29	7.0
30	8.0
31	27.0
32	40.0
33	69.0
34	145.0
35	518.0
36	2759.0
37	402.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.420736288504884	19.158527422990232	14.074630603556223	26.34610568494866
2	20.075000000000003	27.425	33.5	19.0
3	21.85	26.924999999999997	31.15	20.075000000000003
4	24.95	34.575	21.4	19.075
5	25.724999999999998	35.225	22.0	17.05
6	23.0	35.275	23.425	18.3
7	19.975	22.25	38.725	19.05
8	24.15	23.1	26.950000000000003	25.8
9	23.150000000000002	25.924999999999997	26.5	24.425
10-11	26.0	31.9875	22.625	19.3875
12-13	25.2125	26.687499999999996	25.074999999999996	23.025000000000002
14-15	24.7	27.6375	28.1625	19.5
16-17	24.5375	28.237499999999997	28.299999999999997	18.925
18-19	24.4875	29.925	25.35	20.2375
20-21	23.4125	29.375	27.725	19.4875
22-23	24.05	29.1875	25.275	21.4875
24-25	24.337500000000002	29.299999999999997	25.874999999999996	20.4875
26-27	24.1375	28.549999999999997	25.6	21.712500000000002
28-29	23.65	26.900000000000002	27.537499999999998	21.912499999999998
30-31	23.925	28.425	27.150000000000002	20.5
32-33	24.575	29.125	26.887499999999996	19.412499999999998
34-35	23.1375	29.012500000000003	26.4625	21.3875
36-37	23.5	29.349999999999998	27.575	19.575
38-39	23.25	27.525	27.237499999999997	21.987499999999997
40-41	23.400000000000002	27.3	27.750000000000004	21.55
42-43	22.575	29.862499999999997	27.675	19.8875
44-45	24.05	29.525000000000002	27.925	18.5
46-47	23.2875	29.025000000000002	24.462500000000002	23.225
48-49	23.8375	28.65	27.175	20.3375
50-51	22.162499999999998	30.062499999999996	25.224999999999998	22.55
52-53	24.0125	27.925	27.9375	20.125
54-55	26.8375	28.125	25.087500000000002	19.950000000000003
56-57	22.625	28.9875	26.700000000000003	21.6875
58-59	22.237499999999997	28.9875	27.400000000000002	21.375
60-61	22.7	29.3875	25.937500000000004	21.975
62-63	23.0625	28.4375	26.275	22.225
64-65	23.1375	28.65	28.65	19.5625
66-67	22.775000000000002	27.3375	27.037499999999998	22.85
68-69	22.9875	29.7375	28.012500000000003	19.2625
70-71	22.2125	29.299999999999997	28.037499999999998	20.45
72-73	23.575	27.9125	26.487500000000004	22.025
74-75	24.0	27.8875	26.450000000000003	21.6625
76-77	23.025000000000002	28.125	26.487500000000004	22.3625
78-79	23.3125	27.625	27.762500000000003	21.3
80-81	23.6375	27.775	28.3625	20.225
82-83	23.1375	28.5875	27.075	21.2
84-85	22.525000000000002	28.3875	28.000000000000004	21.087500000000002
86-87	22.6	30.1875	25.924999999999997	21.2875
88-89	24.0625	27.900000000000002	26.3625	21.675
90-91	24.0625	28.599999999999998	25.4875	21.85
92-93	24.3	29.975	26.387500000000003	19.3375
94-95	22.2625	30.175	26.35	21.212500000000002
96-97	24.587500000000002	28.299999999999997	25.387500000000003	21.725
98-99	23.674999999999997	28.5875	26.9125	20.825
100-101	25.112499999999997	27.925	26.6125	20.349999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	1.5
24	2.0
25	4.5
26	4.5
27	5.0
28	6.5
29	17.0
30	51.5
31	102.0
32	138.0
33	128.5
34	114.0
35	101.5
36	83.5
37	91.0
38	84.5
39	74.0
40	92.5
41	140.5
42	204.5
43	204.0
44	191.5
45	204.5
46	193.5
47	211.5
48	215.5
49	190.0
50	161.5
51	140.0
52	128.0
53	102.0
54	89.5
55	93.0
56	76.5
57	54.0
58	62.0
59	56.5
60	34.0
61	24.0
62	20.0
63	12.0
64	4.5
65	2.5
66	1.0
67	2.0
68	4.0
69	5.5
70	5.0
71	3.5
72	8.0
73	8.5
74	9.0
75	10.5
76	9.5
77	8.0
78	5.0
79	2.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.143884892086334	31.35
2	21.247002398081534	22.15
3	8.105515587529977	12.675
4	4.172661870503598	8.7
5	1.9664268585131892	5.125
6	1.8225419664268585	5.7
7	0.28776978417266186	1.05
8	0.4316546762589928	1.7999999999999998
9	0.3357314148681055	1.575
>10	1.486810551558753	9.875
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	23	0.575	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	21	0.525	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	17	0.42500000000000004	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	17	0.42500000000000004	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	15	0.375	No Hit
GGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGG	15	0.375	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	15	0.375	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	14	0.35000000000000003	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	14	0.35000000000000003	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	13	0.325	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	13	0.325	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	13	0.325	No Hit
ATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAA	13	0.325	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	13	0.325	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	12	0.3	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	12	0.3	No Hit
GAGGACCTGATGCAGTGCACAGCATTTGCAACCGCAGATGAGTATCACCT	12	0.3	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	11	0.27499999999999997	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	11	0.27499999999999997	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	11	0.27499999999999997	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	10	0.25	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	10	0.25	No Hit
GCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAA	10	0.25	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	10	0.25	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	10	0.25	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	10	0.25	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	10	0.25	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	10	0.25	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	10	0.25	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	10	0.25	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	10	0.25	No Hit
TCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAAT	9	0.22499999999999998	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	9	0.22499999999999998	No Hit
GCATCCCGGACCCGGCAGCCATCCAGGACCAACCTTCCAGATCTGTCTGT	9	0.22499999999999998	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	9	0.22499999999999998	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	9	0.22499999999999998	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	9	0.22499999999999998	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	9	0.22499999999999998	No Hit
TTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATC	8	0.2	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	8	0.2	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	8	0.2	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	8	0.2	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	8	0.2	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	8	0.2	No Hit
GGGAAATCAAGCTAAACTCTGAGCTGGACTTGGATGACGCCATTCTAGAG	8	0.2	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	8	0.2	No Hit
GCTCTTTGCCCTCAGGCACCGAATAAACCTGAGCTCAGATTTCTTGATCA	8	0.2	No Hit
TTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGAT	7	0.17500000000000002	No Hit
TTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAG	7	0.17500000000000002	No Hit
AGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAA	7	0.17500000000000002	No Hit
CACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAA	7	0.17500000000000002	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	7	0.17500000000000002	No Hit
GTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGAT	7	0.17500000000000002	No Hit
AGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGA	6	0.15	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	6	0.15	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	6	0.15	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	6	0.15	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	6	0.15	No Hit
GTCTGTCTCAAGAGCTGGTCTCCTGTGGCTATGTGGAAGTGACAAGCCTG	6	0.15	No Hit
GCTGGCTATTTGGGAAGCAACCCTGGACAAATTTATTGAGTCTATTCAGT	6	0.15	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	6	0.15	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	6	0.15	No Hit
GCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTGAGCTTCCT	6	0.15	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	6	0.15	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	6	0.15	No Hit
GCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCA	6	0.15	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	6	0.15	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	6	0.15	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	6	0.15	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	6	0.15	No Hit
CACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAA	6	0.15	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	6	0.15	No Hit
GTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTG	6	0.15	No Hit
ACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTG	6	0.15	No Hit
CAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCC	6	0.15	No Hit
AATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAAT	6	0.15	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	6	0.15	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	6	0.15	No Hit
TCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTTGG	6	0.15	No Hit
ACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTC	6	0.15	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	6	0.15	No Hit
GGCAAAGAACACTGGCCAAGCATGCACCCCTTTGGGACTCACAGGAGCAT	6	0.15	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	6	0.15	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	6	0.15	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	6	0.15	No Hit
GAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGG	6	0.15	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	6	0.15	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	6	0.15	No Hit
GTGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAG	6	0.15	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	6	0.15	No Hit
GGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGG	6	0.15	No Hit
TTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGT	5	0.125	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	5	0.125	No Hit
CCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCA	5	0.125	No Hit
CTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGA	5	0.125	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	5	0.125	No Hit
GGGGAAATCAAGCTAAACTCTGAGCTGGACTTGGATGACGCCATTCTAGA	5	0.125	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
GTGTAATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAAC	5	0.125	No Hit
GAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAA	5	0.125	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	5	0.125	No Hit
CAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATAC	5	0.125	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	5	0.125	No Hit
CAAACTGTCTCATAAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGCCC	5	0.125	No Hit
TGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGG	5	0.125	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	5	0.125	No Hit
GACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCT	5	0.125	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	5	0.125	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	5	0.125	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	5	0.125	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	5	0.125	No Hit
CCGACGTTGAAAAGGAGGAAACAGAGACCAAGACAGAGCAAGCCAGTGAA	5	0.125	No Hit
CCTCAATGAGAAGCGAGCACTCCGCTTGGAGTGGATGATCGTCATCCTCA	5	0.125	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	5	0.125	No Hit
GGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGTTG	5	0.125	No Hit
ACGAAACCCAGCCCTATGAAGTGGCTCTCGTTCACTGGGAGAACGAAGAA	5	0.125	No Hit
GCTGTCCATTGGTTGGACGGCAAAGAACACTGGCCAAGCATGCACCCCTT	5	0.125	No Hit
GAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGG	5	0.125	No Hit
CCAATGTCGGCAATACCTAGTCCCACTTCTCAAACTGGAGCCTCCTCCTT	5	0.125	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	5	0.125	No Hit
ATCAGACACAAGCAGACTCTTTCCGTTAAATGCTGTCCATTGGTTGGACG	5	0.125	No Hit
CTTCCTTCTCACAGAAACAAAGTCAGAAAATACAGCTTCTCAGAGATAAA	5	0.125	No Hit
TGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGG	5	0.125	No Hit
AGCACGTGATGCAAGTTCTAGAGAGACACGAAACCCAGCCCTATGAAGTG	5	0.125	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	5	0.125	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	5	0.125	No Hit
GGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAA	5	0.125	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	5	0.125	No Hit
CTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTC	5	0.125	No Hit
GGACGACCTACAGGAGCCAAAGGGAGACCTGACAGAATCCTAAGACGTGA	5	0.125	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0625	0.0	0.0	0.0	0.0
72-73	0.1375	0.0	0.0	0.0	0.0
74-75	0.175	0.0	0.0	0.0	0.0
76-77	0.35	0.0	0.0	0.0	0.0
78-79	0.45	0.0	0.0	0.0	0.0
80-81	0.4625	0.0	0.0	0.0	0.0
82-83	0.6125	0.0	0.0	0.0	0.0
84-85	0.825	0.0	0.0	0.0	0.0
86-87	1.0625	0.0	0.0	0.0	0.0
88-89	1.1875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGAAC	15	6.142176E-4	95.0	6
CAACCAA	15	6.142176E-4	95.0	2
CCAAGAA	15	6.142176E-4	95.0	5
AGAACTA	20	0.0019257745	71.25	8
AACCAAG	20	0.0019257745	71.25	3
GAACTAT	20	0.0019257745	71.25	9
CCAACCA	25	0.0046641747	57.0	1
ACCAAGA	25	0.0046641747	57.0	4
GCAGGTA	15	0.009957196	47.5	20-21
CATCCAC	15	0.009957196	47.5	26-27
AACTATC	15	0.009957196	47.5	10-11
AAGAACT	30	0.009594663	47.5	7
CAAAGCA	15	0.009957196	47.5	16-17
CAGGTAC	15	0.009957196	47.5	20-21
AGGTACA	15	0.009957196	47.5	22-23
ATCAAAG	15	0.009957196	47.5	14-15
GGTACAT	15	0.009957196	47.5	22-23
ACTATCA	15	0.009957196	47.5	10-11
CTATCAA	15	0.009957196	47.5	12-13
CACAAGG	25	0.001521768	38.0	30-31
>>END_MODULE
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
Read 4095132 spots for SRR24882420.sra
Written 4095132 spots for SRR24882420.sra
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
Read 4095129 spots for SRR24882420.sra
Written 4095129 spots for SRR24882420.sra
SRR ids: ['SRR24882420.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_1w9ie9lh
SRR24882420.sra spots: 32761035
blocks: [[1, 4095129], [4095130, 8190258], [8190259, 12285387], [12285388, 16380516], [16380517, 20475645], [20475646, 24570774], [24570775, 28665903], [28665904, 32761035]]
SRR24882420 file size 7912616
SRR24882420 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882420 SRR24882420_1.fastq SRR24882420_2.fastq
Input file:	SRR24882420_1.fastq
Paired file:	SRR24882420_2.fastq
trimmed:	SRR24882420-trimmed-pair1.fastq, SRR24882420-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 02:21:05 2026 >> started

Fri Jul  3 02:21:30 2026 >> done (24.666s)
32761035 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
32761035 (100.00%) read pairs available; of these:
  604919 ( 1.85%) trimmed read pairs available after processing
32156116 (98.15%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 62	       2	  0.00%
 63	       1	  0.00%
 64	       1	  0.00%
 65	       0	  0.00%
 66	      18	  0.00%
 67	    1034	  0.00%
 68	    5894	  0.02%
 69	    7259	  0.02%
 70	    7648	  0.02%
 71	    8378	  0.03%
 72	    9201	  0.03%
 73	    9600	  0.03%
 74	   10085	  0.03%
 75	   11040	  0.03%
 76	   11584	  0.04%
 77	   12254	  0.04%
 78	   12283	  0.04%
 79	   13115	  0.04%
 80	   13799	  0.04%
 81	   14639	  0.04%
 82	   14905	  0.05%
 83	   15779	  0.05%
 84	   16689	  0.05%
 85	   17110	  0.05%
 86	   17615	  0.05%
 87	   18557	  0.06%
 88	   19740	  0.06%
 89	   19978	  0.06%
 90	   21546	  0.07%
 91	   22824	  0.07%
 92	   22830	  0.07%
 93	   24258	  0.07%
 94	   24859	  0.08%
 95	   25826	  0.08%
 96	   26629	  0.08%
 97	   28981	  0.09%
 98	   29108	  0.09%
 99	   31394	  0.10%
100	   58456	  0.18%
101	32156116	 98.15%
32761035 reads passed initial QC


criterion=sequence-density
sequence-density=0.56
sequence-density-rank=1
fanout-score=2.65
fanout-score-rank=26
prefix-density=0.63
prefix-fanout=2.4
sequence=TTCAGGTCTCCCTCCA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=43.36
fanout-score-rank=1
prefix-density=0.45
prefix-fanout=2.3
sequence=CAAAGATCTTCACATTTTGCTCATCTCTTTGGTCACTTTCCTGCACTTGGATCAATCACTCTTCGCTTATGATTTTGGTGTGAACCTCCCGGCTCTTCACCCGCAGCTTGTTGACCTGGGACTCCGCGATGTCAGCCCGCTCCTCGGCTTCCTCCAGCTCGTGCTGGATCTTGCGGAA


criterion=sequence-density
sequence-density=1.40
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=31
prefix-density=1.36
prefix-fanout=2.0
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.29
sequence-density-rank=10
fanout-score=24.81
fanout-score-rank=1
prefix-density=1.23
prefix-fanout=5.8
sequence=GAGGAGCTGGAGGAGG
SRR24882420 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 02:21:44
                             Started mapping on |	Jul 03 02:21:44
                                    Finished on |	Jul 03 02:38:22
       Mapping speed, Million of reads per hour |	118.18

                          Number of input reads |	32761035
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27708762
                        Uniquely mapped reads % |	84.58%
                          Average mapped length |	200.77
                       Number of splices: Total |	18559942
            Number of splices: Annotated (sjdb) |	18417502
                       Number of splices: GT/AG |	18422524
                       Number of splices: GC/AG |	108286
                       Number of splices: AT/AC |	10780
               Number of splices: Non-canonical |	18352
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.71
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.64
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5045418
             % of reads mapped to multiple loci |	15.40%
        Number of reads mapped to too many loci |	2728
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6855	6855	6855
N_multimapping	5045418	5045418	5045418
N_noFeature	1486414	25254754	2093656
N_ambiguous	2130220	567446	257627
UnstrandedReadsAssigned:24092128 PositiveStrandReadsAssigned:1886562 NegativeStrandReadsAssigned:25357479
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882420 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882420-trimmed-pair1.fastq
                             SRR24882420-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 32,761,035 reads, 28,464,880 reads pseudoaligned
[quant] estimated average fragment length: 216.464
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,298 rounds

 109283 SRR24882420.ke.tsv
  52636 SRR24882420.se.tsv
 161919 total
==> SRR24882420.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.25	0	0
ENSMUST00000196221.1	9	2	0	0
ENSMUST00000179664.1	11	2.5	0	0
ENSMUST00000178537.1	12	3.5	0	0
ENSMUST00000178862.1	14	5.5	0	0
ENSMUST00000179520.1	11	2.5	0	0
ENSMUST00000179883.1	16	4.25	0	0
ENSMUST00000195858.1	10	3	0	0
ENSMUST00000179932.1	12	3.5	0	0

==> SRR24882420.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	1
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882420 completed mapping pipeline successfully
