Starting /dee2/code/volunteer_pipeline.sh SRR24882410
    current disk space = 438593580032
    free memory = 330887296 
SRR24882410 SRAfilesize
9e03b2cc22efb315e4fc8de2b0a6ed2f  SRR24882410.sra
SRR24882410.sra file validated
SRR24882410 is paired end
SRR24882410 is conventional basespace
SRR24882410 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5665	37.0	37.0	37.0	37.0	37.0
2	36.517	37.0	37.0	37.0	37.0	37.0
3	36.60325	37.0	37.0	37.0	37.0	37.0
4	36.5815	37.0	37.0	37.0	37.0	37.0
5	36.608	37.0	37.0	37.0	37.0	37.0
6	36.6365	37.0	37.0	37.0	37.0	37.0
7	36.604	37.0	37.0	37.0	37.0	37.0
8	36.609	37.0	37.0	37.0	37.0	37.0
9	36.644	37.0	37.0	37.0	37.0	37.0
10-11	36.6275	37.0	37.0	37.0	37.0	37.0
12-13	36.6105	37.0	37.0	37.0	37.0	37.0
14-15	36.63675	37.0	37.0	37.0	37.0	37.0
16-17	36.6065	37.0	37.0	37.0	37.0	37.0
18-19	36.63175	37.0	37.0	37.0	37.0	37.0
20-21	36.551500000000004	37.0	37.0	37.0	37.0	37.0
22-23	36.624	37.0	37.0	37.0	37.0	37.0
24-25	36.599500000000006	37.0	37.0	37.0	37.0	37.0
26-27	36.564750000000004	37.0	37.0	37.0	37.0	37.0
28-29	36.530249999999995	37.0	37.0	37.0	37.0	37.0
30-31	36.577	37.0	37.0	37.0	37.0	37.0
32-33	36.471000000000004	37.0	37.0	37.0	37.0	37.0
34-35	36.5385	37.0	37.0	37.0	37.0	37.0
36-37	36.518	37.0	37.0	37.0	37.0	37.0
38-39	36.44775	37.0	37.0	37.0	37.0	37.0
40-41	36.54925	37.0	37.0	37.0	37.0	37.0
42-43	36.51775	37.0	37.0	37.0	37.0	37.0
44-45	36.4615	37.0	37.0	37.0	37.0	37.0
46-47	36.457	37.0	37.0	37.0	37.0	37.0
48-49	36.469	37.0	37.0	37.0	37.0	37.0
50-51	36.47	37.0	37.0	37.0	37.0	37.0
52-53	36.477000000000004	37.0	37.0	37.0	37.0	37.0
54-55	36.37175	37.0	37.0	37.0	37.0	37.0
56-57	36.39875	37.0	37.0	37.0	37.0	37.0
58-59	36.44075	37.0	37.0	37.0	37.0	37.0
60-61	36.38775	37.0	37.0	37.0	37.0	37.0
62-63	36.35	37.0	37.0	37.0	37.0	37.0
64-65	36.40475	37.0	37.0	37.0	37.0	37.0
66-67	36.37175	37.0	37.0	37.0	37.0	37.0
68-69	36.3835	37.0	37.0	37.0	37.0	37.0
70-71	36.3715	37.0	37.0	37.0	37.0	37.0
72-73	36.32275	37.0	37.0	37.0	37.0	37.0
74-75	36.320750000000004	37.0	37.0	37.0	37.0	37.0
76-77	36.29975	37.0	37.0	37.0	37.0	37.0
78-79	36.251999999999995	37.0	37.0	37.0	37.0	37.0
80-81	36.29775	37.0	37.0	37.0	37.0	37.0
82-83	36.221000000000004	37.0	37.0	37.0	37.0	37.0
84-85	36.2555	37.0	37.0	37.0	37.0	37.0
86-87	36.31325	37.0	37.0	37.0	37.0	37.0
88-89	36.266	37.0	37.0	37.0	37.0	37.0
90-91	36.207750000000004	37.0	37.0	37.0	37.0	37.0
92-93	36.20425	37.0	37.0	37.0	37.0	37.0
94-95	36.117000000000004	37.0	37.0	37.0	37.0	37.0
96-97	36.096000000000004	37.0	37.0	37.0	37.0	37.0
98-99	36.180499999999995	37.0	37.0	37.0	37.0	37.0
100-101	35.96325	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	0.0
24	0.0
25	2.0
26	6.0
27	3.0
28	6.0
29	12.0
30	22.0
31	20.0
32	33.0
33	48.0
34	83.0
35	257.0
36	2623.0
37	884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.475	10.075000000000001	11.85	40.6
2	18.125	16.525000000000002	34.699999999999996	30.65
3	18.579644911227806	19.654913728432106	31.182795698924732	30.582645661415352
4	25.324999999999996	26.25	23.425	25.0
5	22.5	29.175	26.650000000000002	21.675
6	19.425	32.025	27.925	20.625
7	16.025	22.675	44.125	17.175
8	21.25	21.975	31.674999999999997	25.1
9	20.4	22.55	33.800000000000004	23.25
10-11	21.6625	31.5	24.05	22.787499999999998
12-13	22.125	23.375	29.5375	24.962500000000002
14-15	21.75	27.474999999999998	28.499999999999996	22.275
16-17	20.6125	26.887499999999996	27.750000000000004	24.75
18-19	22.5625	27.287499999999998	26.237500000000004	23.9125
20-21	22.900000000000002	26.187500000000004	28.0625	22.85
22-23	22.6375	26.6	26.437500000000004	24.325
24-25	24.224999999999998	25.874999999999996	26.775	23.125
26-27	22.7	28.1875	26.75	22.3625
28-29	21.15	27.325	28.537499999999998	22.9875
30-31	21.837500000000002	26.637499999999996	28.7	22.825
32-33	21.2625	25.7375	30.125	22.875
34-35	20.849999999999998	26.937499999999996	27.35	24.8625
36-37	22.6125	25.687500000000004	28.9	22.8
38-39	22.2125	27.950000000000003	29.099999999999998	20.7375
40-41	21.212500000000002	28.825	27.725	22.237499999999997
42-43	22.2	30.2	25.6	22.0
44-45	19.475	29.4	27.55	23.575
46-47	23.8875	29.075	23.599999999999998	23.4375
48-49	21.8875	28.975	26.1625	22.975
50-51	22.8125	26.650000000000002	27.900000000000002	22.6375
52-53	23.0625	24.95	27.8625	24.125
54-55	21.275	24.95	28.625	25.15
56-57	20.1125	27.4125	29.15	23.325000000000003
58-59	21.65	25.4	28.0625	24.887500000000003
60-61	20.1375	28.9125	27.05	23.9
62-63	23.4875	27.150000000000002	26.887499999999996	22.475
64-65	23.1625	24.75	28.3875	23.7
66-67	24.6625	25.7625	27.787499999999998	21.7875
68-69	23.375	26.450000000000003	26.775	23.400000000000002
70-71	23.2125	27.0875	27.200000000000003	22.5
72-73	21.6	27.250000000000004	27.5875	23.5625
74-75	21.8125	28.6375	28.199999999999996	21.349999999999998
76-77	24.212500000000002	26.4125	26.5875	22.787499999999998
78-79	23.7125	26.125	27.400000000000002	22.7625
80-81	24.425	25.2625	27.750000000000004	22.5625
82-83	24.099999999999998	27.35	26.775	21.775
84-85	20.8125	28.712500000000002	26.5125	23.962500000000002
86-87	23.7	27.85	25.924999999999997	22.525000000000002
88-89	24.337500000000002	26.275	28.012500000000003	21.375
90-91	23.1375	25.2625	28.625	22.975
92-93	22.912499999999998	26.237500000000004	27.700000000000003	23.150000000000002
94-95	22.775000000000002	28.075	26.950000000000003	22.2
96-97	21.375	27.1	25.0	26.525
98-99	21.675	27.1	27.950000000000003	23.275000000000002
100-101	22.3625	26.35	28.5875	22.7
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0
22	3.5
23	2.0
24	2.0
25	11.0
26	17.5
27	10.0
28	3.0
29	6.5
30	31.0
31	74.0
32	104.5
33	107.5
34	101.5
35	121.0
36	124.5
37	106.5
38	81.0
39	66.5
40	86.0
41	146.5
42	199.5
43	171.5
44	133.0
45	135.5
46	199.5
47	239.0
48	178.5
49	131.5
50	134.5
51	125.5
52	111.5
53	111.5
54	100.5
55	103.5
56	96.0
57	73.0
58	90.5
59	126.0
60	119.5
61	70.5
62	41.0
63	24.5
64	9.0
65	3.5
66	5.0
67	6.5
68	4.5
69	5.0
70	4.5
71	3.5
72	5.5
73	7.0
74	5.5
75	2.0
76	3.5
77	4.5
78	4.0
79	2.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.73239436619718	36.6
2	16.19718309859155	17.25
3	6.009389671361502	9.6
4	2.5821596244131455	5.5
5	1.9248826291079812	5.125
6	0.892018779342723	2.85
7	0.7511737089201878	2.8000000000000003
8	0.42253521126760557	1.7999999999999998
9	0.4694835680751174	2.25
>10	2.0187793427230045	16.225
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	50	1.25	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	45	1.125	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	26	0.65	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	23	0.575	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	23	0.575	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	22	0.5499999999999999	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	20	0.5	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	18	0.44999999999999996	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	16	0.4	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	16	0.4	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	16	0.4	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	16	0.4	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	16	0.4	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	15	0.375	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	14	0.35000000000000003	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	14	0.35000000000000003	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	14	0.35000000000000003	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	13	0.325	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	12	0.3	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	12	0.3	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	12	0.3	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	12	0.3	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	12	0.3	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	12	0.3	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	12	0.3	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	11	0.27499999999999997	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	11	0.27499999999999997	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	11	0.27499999999999997	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	11	0.27499999999999997	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	11	0.27499999999999997	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	11	0.27499999999999997	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	11	0.27499999999999997	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	11	0.27499999999999997	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	10	0.25	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	10	0.25	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	10	0.25	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	10	0.25	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	10	0.25	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	10	0.25	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	10	0.25	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	10	0.25	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	10	0.25	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	10	0.25	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	9	0.22499999999999998	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	9	0.22499999999999998	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	9	0.22499999999999998	No Hit
GGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGC	9	0.22499999999999998	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	9	0.22499999999999998	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	9	0.22499999999999998	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	9	0.22499999999999998	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	9	0.22499999999999998	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	9	0.22499999999999998	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	9	0.22499999999999998	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	8	0.2	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	8	0.2	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	8	0.2	No Hit
CTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAGAAA	8	0.2	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	8	0.2	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	8	0.2	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	8	0.2	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	8	0.2	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	8	0.2	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	7	0.17500000000000002	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	7	0.17500000000000002	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	7	0.17500000000000002	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	7	0.17500000000000002	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	7	0.17500000000000002	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	7	0.17500000000000002	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	7	0.17500000000000002	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	7	0.17500000000000002	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	7	0.17500000000000002	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	7	0.17500000000000002	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	7	0.17500000000000002	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	7	0.17500000000000002	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	7	0.17500000000000002	No Hit
TTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCC	7	0.17500000000000002	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	7	0.17500000000000002	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	7	0.17500000000000002	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	6	0.15	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	6	0.15	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	6	0.15	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
GTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTC	6	0.15	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	6	0.15	No Hit
CTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGT	6	0.15	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	6	0.15	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	6	0.15	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	6	0.15	No Hit
AAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACA	6	0.15	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	6	0.15	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	6	0.15	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	6	0.15	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	6	0.15	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	6	0.15	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	6	0.15	No Hit
AGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTT	6	0.15	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	5	0.125	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	5	0.125	No Hit
CACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGA	5	0.125	No Hit
CTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCT	5	0.125	No Hit
CTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGATCCATTCT	5	0.125	No Hit
GCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTAG	5	0.125	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	5	0.125	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	5	0.125	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	5	0.125	No Hit
CAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATG	5	0.125	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	5	0.125	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	5	0.125	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	5	0.125	No Hit
GATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCATTAA	5	0.125	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	5	0.125	No Hit
GTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGC	5	0.125	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	5	0.125	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	5	0.125	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	5	0.125	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	5	0.125	No Hit
GTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTG	5	0.125	No Hit
GGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGA	5	0.125	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	5	0.125	No Hit
AGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	5	0.125	No Hit
TACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAA	5	0.125	No Hit
GTCACAATCAGGACCATGAACCACTGAGAGAAACCAGAGATGAAACCAGA	5	0.125	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	5	0.125	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	5	0.125	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	5	0.125	No Hit
GTCCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATA	5	0.125	No Hit
GTGACTTCAGGAACTTCAAGGCTCTCTGGGGTAGCCTGTACAGTCTCGGA	5	0.125	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	5	0.125	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	5	0.125	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	5	0.125	No Hit
ATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCA	5	0.125	No Hit
TGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCT	5	0.125	No Hit
CCCCACATGGGTAAGATAGCAAATGAACAGGTTCAGGAGCTTCTGACACC	5	0.125	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	5	0.125	No Hit
GTCTTCATCAGCTCCTCCACAGGCTCAGCTTCCTGGGGGACCTCCTGGGT	5	0.125	No Hit
CTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAA	5	0.125	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.1	0.0	0.0	0.0	0.0
72-73	0.175	0.0	0.0	0.0	0.0
74-75	0.3125	0.0	0.0	0.0	0.0
76-77	0.42500000000000004	0.0	0.0	0.0	0.0
78-79	0.5249999999999999	0.0	0.0	0.0	0.0
80-81	0.675	0.0	0.0	0.0	0.0
82-83	0.7749999999999999	0.0	0.0	0.0	0.0
84-85	0.975	0.0	0.0	0.0	0.0
86-87	1.125	0.0	0.0	0.0	0.0
88-89	1.3250000000000002	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882410 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882410_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.31375	37.0	37.0	37.0	37.0	37.0
2	36.262	37.0	37.0	37.0	37.0	37.0
3	36.3355	37.0	37.0	37.0	37.0	37.0
4	36.3525	37.0	37.0	37.0	37.0	37.0
5	36.3525	37.0	37.0	37.0	37.0	37.0
6	36.411	37.0	37.0	37.0	37.0	37.0
7	36.4095	37.0	37.0	37.0	37.0	37.0
8	36.487	37.0	37.0	37.0	37.0	37.0
9	36.483	37.0	37.0	37.0	37.0	37.0
10-11	36.42525	37.0	37.0	37.0	37.0	37.0
12-13	36.429	37.0	37.0	37.0	37.0	37.0
14-15	36.4185	37.0	37.0	37.0	37.0	37.0
16-17	36.37475	37.0	37.0	37.0	37.0	37.0
18-19	36.31125	37.0	37.0	37.0	37.0	37.0
20-21	36.35875	37.0	37.0	37.0	37.0	37.0
22-23	36.306749999999994	37.0	37.0	37.0	37.0	37.0
24-25	36.33625	37.0	37.0	37.0	37.0	37.0
26-27	36.31925	37.0	37.0	37.0	37.0	37.0
28-29	36.27675000000001	37.0	37.0	37.0	37.0	37.0
30-31	36.40525	37.0	37.0	37.0	37.0	37.0
32-33	36.3075	37.0	37.0	37.0	37.0	37.0
34-35	36.3125	37.0	37.0	37.0	37.0	37.0
36-37	36.26625	37.0	37.0	37.0	37.0	37.0
38-39	36.208749999999995	37.0	37.0	37.0	37.0	37.0
40-41	36.234	37.0	37.0	37.0	37.0	37.0
42-43	36.2385	37.0	37.0	37.0	37.0	37.0
44-45	36.126000000000005	37.0	37.0	37.0	37.0	37.0
46-47	36.1875	37.0	37.0	37.0	37.0	37.0
48-49	36.08175	37.0	37.0	37.0	37.0	37.0
50-51	36.14	37.0	37.0	37.0	37.0	37.0
52-53	36.212	37.0	37.0	37.0	37.0	37.0
54-55	36.060249999999996	37.0	37.0	37.0	37.0	37.0
56-57	36.12375	37.0	37.0	37.0	37.0	37.0
58-59	36.113749999999996	37.0	37.0	37.0	37.0	37.0
60-61	36.11525	37.0	37.0	37.0	37.0	37.0
62-63	36.0025	37.0	37.0	37.0	37.0	37.0
64-65	36.155	37.0	37.0	37.0	37.0	37.0
66-67	36.06125	37.0	37.0	37.0	37.0	37.0
68-69	36.033	37.0	37.0	37.0	37.0	37.0
70-71	35.8405	37.0	37.0	37.0	37.0	37.0
72-73	35.925749999999994	37.0	37.0	37.0	37.0	37.0
74-75	36.0045	37.0	37.0	37.0	37.0	37.0
76-77	35.9825	37.0	37.0	37.0	37.0	37.0
78-79	35.88575	37.0	37.0	37.0	37.0	37.0
80-81	35.86475	37.0	37.0	37.0	37.0	37.0
82-83	35.93425	37.0	37.0	37.0	37.0	37.0
84-85	35.845749999999995	37.0	37.0	37.0	37.0	37.0
86-87	35.8155	37.0	37.0	37.0	37.0	37.0
88-89	35.70725	37.0	37.0	37.0	37.0	37.0
90-91	35.776250000000005	37.0	37.0	37.0	37.0	37.0
92-93	35.797250000000005	37.0	37.0	37.0	37.0	37.0
94-95	35.749	37.0	37.0	37.0	37.0	37.0
96-97	35.792	37.0	37.0	37.0	37.0	37.0
98-99	35.706	37.0	37.0	37.0	37.0	37.0
100-101	35.389250000000004	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	4.0
26	4.0
27	10.0
28	14.0
29	14.0
30	16.0
31	29.0
32	43.0
33	94.0
34	162.0
35	521.0
36	2751.0
37	337.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.45181476846058	19.474342928660825	13.667083854818523	25.406758448060074
2	21.025	27.425	32.0	19.55
3	20.75	26.900000000000002	30.95	21.4
4	25.124999999999996	33.15	21.25	20.474999999999998
5	26.474999999999998	31.55	23.825	18.15
6	24.0	34.25	22.55	19.2
7	21.6	22.625	36.199999999999996	19.575
8	23.9	22.125	28.799999999999997	25.174999999999997
9	24.325	24.224999999999998	27.250000000000004	24.2
10-11	24.887500000000003	31.55	21.8125	21.75
12-13	26.137500000000003	24.8125	26.1125	22.9375
14-15	23.5125	25.5375	28.825	22.125
16-17	25.387500000000003	27.750000000000004	28.3125	18.55
18-19	24.275	28.625	26.3125	20.7875
20-21	23.825	28.8375	27.325	20.0125
22-23	23.35	28.675	26.5375	21.4375
24-25	23.2625	29.1625	25.575	22.0
26-27	24.1625	28.625	25.2	22.0125
28-29	23.9	27.375	26.5875	22.1375
30-31	23.974999999999998	27.5625	26.6625	21.8
32-33	22.9625	27.925	27.4125	21.7
34-35	24.05	27.0625	25.662499999999998	23.225
36-37	23.724999999999998	27.2625	27.975	21.0375
38-39	23.4125	27.450000000000003	26.487500000000004	22.650000000000002
40-41	23.05	28.65	26.775	21.525
42-43	23.5625	29.3875	25.4	21.65
44-45	23.2625	28.1625	27.6125	20.962500000000002
46-47	24.525	27.650000000000002	24.462500000000002	23.3625
48-49	23.6875	26.775	28.512500000000003	21.025
50-51	22.2125	27.3625	27.3125	23.1125
52-53	24.337500000000002	26.8625	27.775	21.025
54-55	25.025	26.8625	26.25	21.8625
56-57	21.512500000000003	28.825	26.3	23.3625
58-59	20.7375	28.15	26.9125	24.2
60-61	21.987499999999997	28.5625	27.6375	21.8125
62-63	23.275000000000002	28.975	25.25	22.5
64-65	23.175	27.250000000000004	29.049999999999997	20.525
66-67	22.625	26.075	26.5875	24.712500000000002
68-69	23.2125	29.1625	25.5	22.125
70-71	22.775000000000002	29.1375	26.687499999999996	21.4
72-73	23.075000000000003	28.349999999999998	25.637500000000003	22.9375
74-75	23.6375	27.8875	25.8625	22.6125
76-77	22.475	27.1375	25.224999999999998	25.162499999999998
78-79	24.0625	26.687499999999996	27.6625	21.587500000000002
80-81	23.1125	26.7625	27.650000000000002	22.475
82-83	22.6375	27.025	28.1875	22.15
84-85	21.65	26.400000000000002	29.125	22.825
86-87	23.7875	27.6125	25.7125	22.8875
88-89	24.712500000000002	27.6625	26.0625	21.5625
90-91	23.8625	27.3625	26.150000000000002	22.625
92-93	23.549999999999997	28.299999999999997	26.625	21.525
94-95	23.150000000000002	29.9375	26.075	20.837500000000002
96-97	23.549999999999997	28.575	25.8125	22.0625
98-99	23.3375	28.3875	25.674999999999997	22.6
100-101	24.15	27.224999999999998	26.924999999999997	21.7
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.0
19	0.5
20	0.5
21	0.0
22	0.5
23	1.5
24	1.5
25	2.5
26	3.0
27	1.5
28	3.5
29	10.5
30	34.5
31	80.5
32	130.0
33	124.0
34	99.0
35	86.0
36	82.0
37	104.0
38	92.5
39	82.5
40	106.5
41	138.0
42	187.0
43	204.0
44	195.5
45	211.0
46	208.5
47	189.0
48	163.5
49	142.5
50	143.5
51	121.0
52	90.0
53	84.0
54	88.5
55	122.0
56	115.0
57	76.5
58	91.5
59	94.0
60	67.0
61	42.5
62	34.0
63	27.5
64	13.0
65	5.5
66	2.0
67	4.5
68	6.0
69	6.0
70	5.5
71	9.0
72	9.0
73	7.5
74	9.0
75	8.0
76	8.5
77	11.0
78	8.0
79	1.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.90676826535186	37.875
2	16.808606006275213	18.75
3	5.647691618108472	9.45
4	3.9444195428059166	8.799999999999999
5	1.523980277902286	4.25
6	1.2102196324518153	4.05
7	0.8516360376512775	3.325
8	0.49305244285073957	2.1999999999999997
9	0.3585835948005379	1.7999999999999998
>10	1.2550425818018824	9.5
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	42	1.05	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	26	0.65	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	20	0.5	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	18	0.44999999999999996	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	15	0.375	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	13	0.325	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	13	0.325	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	13	0.325	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	12	0.3	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	12	0.3	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	12	0.3	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	12	0.3	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	12	0.3	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	12	0.3	No Hit
ACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGG	12	0.3	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	12	0.3	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	11	0.27499999999999997	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	11	0.27499999999999997	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	11	0.27499999999999997	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	11	0.27499999999999997	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	10	0.25	No Hit
ATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCT	10	0.25	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	10	0.25	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	10	0.25	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	10	0.25	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	10	0.25	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	10	0.25	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	10	0.25	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	9	0.22499999999999998	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	9	0.22499999999999998	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	9	0.22499999999999998	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	9	0.22499999999999998	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	9	0.22499999999999998	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	9	0.22499999999999998	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	9	0.22499999999999998	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	9	0.22499999999999998	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	8	0.2	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	8	0.2	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	8	0.2	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	8	0.2	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	8	0.2	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	8	0.2	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	8	0.2	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	8	0.2	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	8	0.2	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	8	0.2	No Hit
GAGCAGTTGGCCTCCGAGATCGAACCAAGTAGAGAGGAATCTTGGGTTTC	8	0.2	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	7	0.17500000000000002	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	7	0.17500000000000002	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	7	0.17500000000000002	No Hit
GCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGT	7	0.17500000000000002	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	7	0.17500000000000002	No Hit
CTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCAT	7	0.17500000000000002	No Hit
GCAGAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGAT	7	0.17500000000000002	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	7	0.17500000000000002	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	7	0.17500000000000002	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	7	0.17500000000000002	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	7	0.17500000000000002	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	7	0.17500000000000002	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	7	0.17500000000000002	No Hit
CATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTC	7	0.17500000000000002	No Hit
AGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAA	7	0.17500000000000002	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	7	0.17500000000000002	No Hit
TTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATT	7	0.17500000000000002	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	7	0.17500000000000002	No Hit
CCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACC	7	0.17500000000000002	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	6	0.15	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	6	0.15	No Hit
AGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTC	6	0.15	No Hit
TAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTT	6	0.15	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	6	0.15	No Hit
TGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACAC	6	0.15	No Hit
AGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAA	6	0.15	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	6	0.15	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	6	0.15	No Hit
GAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCTC	6	0.15	No Hit
GGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGC	6	0.15	No Hit
CCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCC	6	0.15	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	6	0.15	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	6	0.15	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	6	0.15	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	6	0.15	No Hit
TTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGAT	6	0.15	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	6	0.15	No Hit
CAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCC	6	0.15	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	6	0.15	No Hit
GATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCT	6	0.15	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	6	0.15	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	6	0.15	No Hit
AAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAA	6	0.15	No Hit
GGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAGGAGAGAATCAAGGT	6	0.15	No Hit
ATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTC	6	0.15	No Hit
GAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTG	6	0.15	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	5	0.125	No Hit
GCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAA	5	0.125	No Hit
TGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTG	5	0.125	No Hit
CGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGT	5	0.125	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	5	0.125	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	5	0.125	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	5	0.125	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	5	0.125	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	5	0.125	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	5	0.125	No Hit
TGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTAC	5	0.125	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	5	0.125	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	5	0.125	No Hit
GACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAA	5	0.125	No Hit
GAAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGT	5	0.125	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	5	0.125	No Hit
GTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGT	5	0.125	No Hit
CTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAA	5	0.125	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	5	0.125	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	5	0.125	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	5	0.125	No Hit
CAAGATTGCTCGAGAAGCGCAGTTCGCGTCTGCAGGGCAAAAAACGAGGA	5	0.125	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	5	0.125	No Hit
CCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAG	5	0.125	No Hit
AGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATAC	5	0.125	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	5	0.125	No Hit
TTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGT	5	0.125	No Hit
GCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTAAAAT	5	0.125	No Hit
CCAGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAG	5	0.125	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	5	0.125	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	5	0.125	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	5	0.125	No Hit
ATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGA	5	0.125	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.1	0.0	0.0	0.0	0.0
72-73	0.175	0.0	0.0	0.0	0.0
74-75	0.3125	0.0	0.0	0.0	0.0
76-77	0.42500000000000004	0.0	0.0	0.0	0.0
78-79	0.5249999999999999	0.0	0.0	0.0	0.0
80-81	0.675	0.0	0.0	0.0	0.0
82-83	0.7749999999999999	0.0	0.0	0.0	0.0
84-85	0.975	0.0	0.0	0.0	0.0
86-87	1.125	0.0	0.0	0.0	0.0
88-89	1.3250000000000002	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTGAA	15	0.009957196	47.5	48-49
>>END_MODULE
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033391 spots for SRR24882410.sra
Written 2033391 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
Read 2033380 spots for SRR24882410.sra
Written 2033380 spots for SRR24882410.sra
SRR ids: ['SRR24882410.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_c1n0jc01
SRR24882410.sra spots: 32534091
blocks: [[1, 2033380], [2033381, 4066760], [4066761, 6100140], [6100141, 8133520], [8133521, 10166900], [10166901, 12200280], [12200281, 14233660], [14233661, 16267040], [16267041, 18300420], [18300421, 20333800], [20333801, 22367180], [22367181, 24400560], [24400561, 26433940], [26433941, 28467320], [28467321, 30500700], [30500701, 32534091]]
SRR24882410 file size 1100885
SRR24882410 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24882410 SRR24882410_1.fastq SRR24882410_2.fastq
Input file:	SRR24882410_1.fastq
Paired file:	SRR24882410_2.fastq
trimmed:	SRR24882410-trimmed-pair1.fastq, SRR24882410-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 05:37:40 2026 >> started

Fri Jul  3 05:38:40 2026 >> done (59.967s)
32534091 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       3 ( 0.00%) empty read pairs filtered out after trimming by size control
32534088 (100.00%) read pairs available; of these:
 1207780 ( 3.71%) trimmed read pairs available after processing
31326308 (96.29%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 63	       1	  0.00%
 64	       2	  0.00%
 65	       5	  0.00%
 66	     382	  0.00%
 67	    2347	  0.01%
 68	    9808	  0.03%
 69	   11354	  0.03%
 70	   12912	  0.04%
 71	   14492	  0.04%
 72	   15908	  0.05%
 73	   16915	  0.05%
 74	   17268	  0.05%
 75	   18796	  0.06%
 76	   20611	  0.06%
 77	   22343	  0.07%
 78	   24014	  0.07%
 79	   24197	  0.07%
 80	   25376	  0.08%
 81	   27359	  0.08%
 82	   29871	  0.09%
 83	   30610	  0.09%
 84	   32713	  0.10%
 85	   34648	  0.11%
 86	   36639	  0.11%
 87	   38432	  0.12%
 88	   41307	  0.13%
 89	   42286	  0.13%
 90	   43238	  0.13%
 91	   45701	  0.14%
 92	   47140	  0.14%
 93	   50036	  0.15%
 94	   53376	  0.16%
 95	   54409	  0.17%
 96	   56783	  0.17%
 97	   61408	  0.19%
 98	   64107	  0.20%
 99	   70264	  0.22%
100	  110722	  0.34%
101	31326308	 96.29%
32534088 reads passed initial QC


criterion=sequence-density
sequence-density=0.46
sequence-density-rank=1
fanout-score=2.38
fanout-score-rank=35
prefix-density=0.50
prefix-fanout=2.2
sequence=GATTCTGCCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=119.52
fanout-score-rank=1
prefix-density=0.14
prefix-fanout=5.0
sequence=GGCACAGCTAGACCCCATCCTCAGTACAGCGGCTTGGCCTTTGACCTCTGGACAGAGACCAAGGCGACCAACAAAGCTAACCCTATGACAGACACTCCTGGGGCTTCCTTCTTTTCCTTTCTGAGATGATAGCTATAGAATACTGGCACAAGAAAGAAATCATAAACAAACCAACTTTTAACCCTCACGTTTATTTGGGGAAGAGGGGAGGGAAGTTTGTGGGGTGCCCACCCCCACTGCAGTCCCGAGCCAATGCTTTCTCTCTGTCCCCTTCTTTTGTGGTTGAGGGAACCGGAGCTGGGGGAGCTGGGGGGAGGGGTCTGGGACTAGCAGGTGTGCAGTGCAGGAAGCAGAGAAGGGGATAGGGGCAGATTCGGGGTGCACAGGGTGGAAAGGGGCAGCTAGCGTTGTGTACATTCTGGGCCGAGATTGGTGGCTGTGGCCCAGGGAAATGTTGAAGGTGGGGAAGAACCCTTAGCCTGGGGTGTCCCTCTGGCGTCCCCTAGGCCCA


criterion=sequence-density
sequence-density=0.46
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=36
prefix-density=0.46
prefix-fanout=2.0
sequence=TTGGGCAGAATC


criterion=fanout-score
sequence-density=0.35
sequence-density-rank=2
fanout-score=53.38
fanout-score-rank=1
prefix-density=18.45
prefix-fanout=1.0
sequence=TGCCTGGGGGACGATTGGTGGCC
SRR24882410 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 05:39:23
                             Started mapping on |	Jul 03 05:39:23
                                    Finished on |	Jul 03 05:42:56
       Mapping speed, Million of reads per hour |	549.87

                          Number of input reads |	32534088
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	26469414
                        Uniquely mapped reads % |	81.36%
                          Average mapped length |	200.12
                       Number of splices: Total |	14294046
            Number of splices: Annotated (sjdb) |	14181512
                       Number of splices: GT/AG |	14143396
                       Number of splices: GC/AG |	121754
                       Number of splices: AT/AC |	9805
               Number of splices: Non-canonical |	19091
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.03%
                        Deletion average length |	3.87
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.49
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6052080
             % of reads mapped to multiple loci |	18.60%
        Number of reads mapped to too many loci |	5201
             % of reads mapped to too many loci |	0.02%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.02%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	12594	12594	12594
N_multimapping	6052080	6052080	6052080
N_noFeature	2179559	23232347	3200661
N_ambiguous	2515362	1259962	296794
UnstrandedReadsAssigned:21774493 PositiveStrandReadsAssigned:1977105 NegativeStrandReadsAssigned:22971959
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882410 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882410-trimmed-pair1.fastq
                             SRR24882410-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 32,534,088 reads, 26,507,549 reads pseudoaligned
[quant] estimated average fragment length: 192.313
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,089 rounds

 109283 SRR24882410.ke.tsv
  52636 SRR24882410.se.tsv
 161919 total
==> SRR24882410.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9	0	0
ENSMUST00000196221.1	9	2	0	0
ENSMUST00000179664.1	11	4	0	0
ENSMUST00000178537.1	12	5	0	0
ENSMUST00000178862.1	14	7	0	0
ENSMUST00000179520.1	11	4	0	0
ENSMUST00000179883.1	16	9	0	0
ENSMUST00000195858.1	10	3	0	0
ENSMUST00000179932.1	12	5	0	0

==> SRR24882410.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	12
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	1
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882410 completed mapping pipeline successfully
