Starting /dee2/code/volunteer_pipeline.sh SRR24882408
    current disk space = 438592028672
    free memory = 335919156 
SRR24882408 SRAfilesize
12e1327ed7004f2ed8e778f9801436be  SRR24882408.sra
SRR24882408.sra file validated
SRR24882408 is paired end
SRR24882408 is conventional basespace
SRR24882408 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.53275	37.0	37.0	37.0	37.0	37.0
2	36.511	37.0	37.0	37.0	37.0	37.0
3	36.558	37.0	37.0	37.0	37.0	37.0
4	36.6285	37.0	37.0	37.0	37.0	37.0
5	36.696	37.0	37.0	37.0	37.0	37.0
6	36.5835	37.0	37.0	37.0	37.0	37.0
7	36.5715	37.0	37.0	37.0	37.0	37.0
8	36.6325	37.0	37.0	37.0	37.0	37.0
9	36.5755	37.0	37.0	37.0	37.0	37.0
10-11	36.71825	37.0	37.0	37.0	37.0	37.0
12-13	36.641999999999996	37.0	37.0	37.0	37.0	37.0
14-15	36.61825	37.0	37.0	37.0	37.0	37.0
16-17	36.62025	37.0	37.0	37.0	37.0	37.0
18-19	36.6065	37.0	37.0	37.0	37.0	37.0
20-21	36.657	37.0	37.0	37.0	37.0	37.0
22-23	36.61575	37.0	37.0	37.0	37.0	37.0
24-25	36.63375	37.0	37.0	37.0	37.0	37.0
26-27	36.5625	37.0	37.0	37.0	37.0	37.0
28-29	36.4995	37.0	37.0	37.0	37.0	37.0
30-31	36.5355	37.0	37.0	37.0	37.0	37.0
32-33	36.54775	37.0	37.0	37.0	37.0	37.0
34-35	36.54675	37.0	37.0	37.0	37.0	37.0
36-37	36.6105	37.0	37.0	37.0	37.0	37.0
38-39	36.4735	37.0	37.0	37.0	37.0	37.0
40-41	36.53075	37.0	37.0	37.0	37.0	37.0
42-43	36.55225	37.0	37.0	37.0	37.0	37.0
44-45	36.521249999999995	37.0	37.0	37.0	37.0	37.0
46-47	36.568	37.0	37.0	37.0	37.0	37.0
48-49	36.458749999999995	37.0	37.0	37.0	37.0	37.0
50-51	36.419250000000005	37.0	37.0	37.0	37.0	37.0
52-53	36.50175	37.0	37.0	37.0	37.0	37.0
54-55	36.455	37.0	37.0	37.0	37.0	37.0
56-57	36.50975	37.0	37.0	37.0	37.0	37.0
58-59	36.5075	37.0	37.0	37.0	37.0	37.0
60-61	36.4475	37.0	37.0	37.0	37.0	37.0
62-63	36.39725	37.0	37.0	37.0	37.0	37.0
64-65	36.3825	37.0	37.0	37.0	37.0	37.0
66-67	36.40625	37.0	37.0	37.0	37.0	37.0
68-69	36.319	37.0	37.0	37.0	37.0	37.0
70-71	36.396249999999995	37.0	37.0	37.0	37.0	37.0
72-73	36.36125	37.0	37.0	37.0	37.0	37.0
74-75	36.3585	37.0	37.0	37.0	37.0	37.0
76-77	36.38525	37.0	37.0	37.0	37.0	37.0
78-79	36.3925	37.0	37.0	37.0	37.0	37.0
80-81	36.36125	37.0	37.0	37.0	37.0	37.0
82-83	36.31825	37.0	37.0	37.0	37.0	37.0
84-85	36.32225	37.0	37.0	37.0	37.0	37.0
86-87	36.31675	37.0	37.0	37.0	37.0	37.0
88-89	36.2285	37.0	37.0	37.0	37.0	37.0
90-91	36.3005	37.0	37.0	37.0	37.0	37.0
92-93	36.292249999999996	37.0	37.0	37.0	37.0	37.0
94-95	36.2475	37.0	37.0	37.0	37.0	37.0
96-97	36.18325	37.0	37.0	37.0	37.0	37.0
98-99	36.246750000000006	37.0	37.0	37.0	37.0	37.0
100-101	35.9975	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	2.0
26	0.0
27	7.0
28	14.0
29	9.0
30	14.0
31	18.0
32	36.0
33	45.0
34	76.0
35	203.0
36	2647.0
37	928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.90922730682671	10.077519379844961	12.253063265816454	40.76019004751188
2	18.625	16.675	36.7	28.000000000000004
3	18.41841841841842	18.66866866866867	30.605605605605607	32.30730730730731
4	22.85	27.6	23.549999999999997	26.0
5	24.775	29.95	24.474999999999998	20.8
6	19.175	32.45	28.225	20.150000000000002
7	15.0	23.775	45.2	16.025
8	20.200000000000003	22.075	33.15	24.575
9	20.8	22.725	33.825	22.650000000000002
10-11	21.75	31.424999999999997	25.0625	21.762500000000003
12-13	22.3875	24.425	28.575	24.6125
14-15	21.25	27.9125	27.8625	22.975
16-17	20.325	27.6375	27.250000000000004	24.7875
18-19	22.8	28.1625	25.724999999999998	23.3125
20-21	21.875	28.3125	26.987499999999997	22.825
22-23	22.85	26.525	26.0125	24.6125
24-25	23.8125	27.4125	26.2625	22.5125
26-27	22.225	27.287499999999998	27.525	22.9625
28-29	20.325	27.9375	29.875	21.8625
30-31	21.987499999999997	25.474999999999998	28.849999999999998	23.6875
32-33	21.775	25.8	29.862499999999997	22.5625
34-35	18.512500000000003	27.775	28.675	25.0375
36-37	21.4875	27.05	29.299999999999997	22.162499999999998
38-39	22.162499999999998	27.8375	29.012500000000003	20.9875
40-41	21.95	28.3875	28.925	20.7375
42-43	22.95	28.95	26.5625	21.5375
44-45	20.349999999999998	29.225	27.825	22.6
46-47	22.9375	29.349999999999998	23.400000000000002	24.3125
48-49	21.4375	30.5375	25.5625	22.4625
50-51	22.3625	26.825	27.55	23.2625
52-53	22.6125	24.7375	28.7	23.95
54-55	20.7125	25.2875	29.2875	24.712500000000002
56-57	19.7	27.0125	29.875	23.4125
58-59	21.7875	26.0625	27.737499999999997	24.4125
60-61	20.599999999999998	28.812500000000004	26.525	24.0625
62-63	23.849999999999998	26.5625	27.275	22.3125
64-65	23.375	24.6625	28.512500000000003	23.45
66-67	23.3375	24.762500000000003	29.1375	22.7625
68-69	22.275	26.7125	27.6	23.4125
70-71	23.5875	25.637500000000003	28.025	22.75
72-73	20.0	27.700000000000003	29.1375	23.1625
74-75	21.512500000000003	27.187499999999996	28.5625	22.7375
76-77	24.4125	26.700000000000003	26.1	22.787499999999998
78-79	23.724999999999998	25.7625	28.6625	21.85
80-81	24.1375	25.9875	27.925	21.95
82-83	24.425	26.974999999999998	26.5125	22.0875
84-85	20.974999999999998	28.575	26.150000000000002	24.3
86-87	23.4625	27.4125	26.1125	23.0125
88-89	25.074999999999996	25.137500000000003	28.712500000000002	21.075
90-91	23.4375	25.525	28.175	22.8625
92-93	23.4625	27.0125	26.974999999999998	22.55
94-95	21.75	27.8375	26.5625	23.849999999999998
96-97	22.2125	27.55	24.5125	25.724999999999998
98-99	22.650000000000002	28.1875	26.924999999999997	22.237499999999997
100-101	23.0625	27.3875	27.250000000000004	22.3
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.5
21	0.5
22	1.5
23	3.5
24	3.5
25	12.5
26	21.0
27	10.5
28	3.5
29	11.5
30	32.0
31	57.0
32	87.0
33	106.0
34	102.0
35	135.0
36	130.5
37	95.0
38	71.0
39	47.0
40	86.0
41	166.5
42	237.5
43	211.0
44	144.0
45	140.0
46	215.5
47	254.0
48	174.0
49	111.5
50	116.0
51	120.0
52	117.0
53	114.0
54	110.5
55	108.0
56	88.0
57	69.0
58	84.5
59	118.5
60	105.0
61	60.0
62	33.0
63	17.0
64	6.0
65	3.5
66	4.5
67	4.0
68	3.5
69	5.5
70	4.0
71	1.0
72	2.0
73	3.0
74	5.5
75	8.5
76	6.5
77	4.0
78	2.5
79	0.5
80	1.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.1
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.675000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.90856313497822	34.575
2	17.94871794871795	18.55
3	5.2249637155297535	8.1
4	3.1930333817126266	6.6000000000000005
5	1.3062409288824384	3.375
6	1.3062409288824384	4.05
7	0.8708272859216255	3.15
8	0.5805515239477503	2.4
9	0.5321722302854378	2.475
>10	2.1286889211417512	16.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	42	1.05	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	38	0.95	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	38	0.95	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	25	0.625	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	20	0.5	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	20	0.5	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	18	0.44999999999999996	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	18	0.44999999999999996	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	17	0.42500000000000004	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	17	0.42500000000000004	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	17	0.42500000000000004	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	16	0.4	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	16	0.4	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	15	0.375	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	15	0.375	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	14	0.35000000000000003	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	14	0.35000000000000003	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	14	0.35000000000000003	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	13	0.325	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	13	0.325	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	13	0.325	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	12	0.3	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	12	0.3	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	12	0.3	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	12	0.3	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	12	0.3	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	12	0.3	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	12	0.3	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	12	0.3	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	12	0.3	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	12	0.3	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	11	0.27499999999999997	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	11	0.27499999999999997	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	11	0.27499999999999997	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	11	0.27499999999999997	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	11	0.27499999999999997	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	11	0.27499999999999997	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	10	0.25	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	10	0.25	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	10	0.25	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	10	0.25	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	10	0.25	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	10	0.25	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	10	0.25	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	9	0.22499999999999998	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	9	0.22499999999999998	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	9	0.22499999999999998	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	9	0.22499999999999998	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	9	0.22499999999999998	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	9	0.22499999999999998	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	9	0.22499999999999998	No Hit
GTCCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATA	9	0.22499999999999998	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	9	0.22499999999999998	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	9	0.22499999999999998	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	9	0.22499999999999998	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	8	0.2	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	8	0.2	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	8	0.2	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	8	0.2	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	8	0.2	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	8	0.2	No Hit
GAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTA	8	0.2	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	8	0.2	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	8	0.2	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	8	0.2	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	8	0.2	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	8	0.2	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	7	0.17500000000000002	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	7	0.17500000000000002	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	7	0.17500000000000002	No Hit
GGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGC	7	0.17500000000000002	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	7	0.17500000000000002	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	7	0.17500000000000002	No Hit
AGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	7	0.17500000000000002	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	7	0.17500000000000002	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	7	0.17500000000000002	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	7	0.17500000000000002	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	7	0.17500000000000002	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	7	0.17500000000000002	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	7	0.17500000000000002	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	7	0.17500000000000002	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	7	0.17500000000000002	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	7	0.17500000000000002	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	7	0.17500000000000002	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	7	0.17500000000000002	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	6	0.15	No Hit
TATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAA	6	0.15	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	6	0.15	No Hit
GCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAG	6	0.15	No Hit
GGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATT	6	0.15	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	6	0.15	No Hit
CTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAA	6	0.15	No Hit
CCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGC	6	0.15	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	6	0.15	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	6	0.15	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	6	0.15	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	6	0.15	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	6	0.15	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	6	0.15	No Hit
ATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGG	6	0.15	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	6	0.15	No Hit
GTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGAT	6	0.15	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	6	0.15	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	6	0.15	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	6	0.15	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	6	0.15	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	6	0.15	No Hit
TTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTA	6	0.15	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	6	0.15	No Hit
GTAATTTCGAAGCTCTTTCCTCCTCGCTGTTGGCATCAAGAAGATCATCA	6	0.15	No Hit
CAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGT	6	0.15	No Hit
CTCCCTTTGGCTCCTGTAGGTCGTCCTCTTCTGTCTGGGCCTTGTTCATT	6	0.15	No Hit
CTACCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGA	5	0.125	No Hit
CGCCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAA	5	0.125	No Hit
CACGTGTCAAGTCAGCAGTCTTTTGACTGTTGGGTCCAGTGTGCACAGGC	5	0.125	No Hit
GTCAATTTCTACATCTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGAC	5	0.125	No Hit
GGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGC	5	0.125	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	5	0.125	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	5	0.125	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	5	0.125	No Hit
GGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGA	5	0.125	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	5	0.125	No Hit
CTCCTTTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGGCTAATACTCT	5	0.125	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	5	0.125	No Hit
CCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAA	5	0.125	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	5	0.125	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	5	0.125	No Hit
CATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACT	5	0.125	No Hit
GTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGGA	5	0.125	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	5	0.125	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	5	0.125	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	5	0.125	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	5	0.125	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	5	0.125	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	5	0.125	No Hit
CCACAATGAGGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGA	5	0.125	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	5	0.125	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.037500000000000006	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.1375	0.0	0.0	0.0	0.0
76-77	0.1875	0.0	0.0	0.0	0.0
78-79	0.3625	0.0	0.0	0.0	0.0
80-81	0.4625	0.0	0.0	0.0	0.0
82-83	0.5875	0.0	0.0	0.0	0.0
84-85	0.7124999999999999	0.0	0.0	0.0	0.0
86-87	0.875	0.0	0.0	0.0	0.0
88-89	1.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882408 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882408_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3965	37.0	37.0	37.0	37.0	37.0
2	36.345	37.0	37.0	37.0	37.0	37.0
3	36.439	37.0	37.0	37.0	37.0	37.0
4	36.485	37.0	37.0	37.0	37.0	37.0
5	36.4765	37.0	37.0	37.0	37.0	37.0
6	36.353	37.0	37.0	37.0	37.0	37.0
7	36.494	37.0	37.0	37.0	37.0	37.0
8	36.5535	37.0	37.0	37.0	37.0	37.0
9	36.497	37.0	37.0	37.0	37.0	37.0
10-11	36.47875	37.0	37.0	37.0	37.0	37.0
12-13	36.48925	37.0	37.0	37.0	37.0	37.0
14-15	36.451	37.0	37.0	37.0	37.0	37.0
16-17	36.4825	37.0	37.0	37.0	37.0	37.0
18-19	36.454750000000004	37.0	37.0	37.0	37.0	37.0
20-21	36.417500000000004	37.0	37.0	37.0	37.0	37.0
22-23	36.377250000000004	37.0	37.0	37.0	37.0	37.0
24-25	36.4015	37.0	37.0	37.0	37.0	37.0
26-27	36.3885	37.0	37.0	37.0	37.0	37.0
28-29	36.3535	37.0	37.0	37.0	37.0	37.0
30-31	36.43025	37.0	37.0	37.0	37.0	37.0
32-33	36.379	37.0	37.0	37.0	37.0	37.0
34-35	36.3875	37.0	37.0	37.0	37.0	37.0
36-37	36.4075	37.0	37.0	37.0	37.0	37.0
38-39	36.37325	37.0	37.0	37.0	37.0	37.0
40-41	36.384	37.0	37.0	37.0	37.0	37.0
42-43	36.31175	37.0	37.0	37.0	37.0	37.0
44-45	36.3065	37.0	37.0	37.0	37.0	37.0
46-47	36.35175	37.0	37.0	37.0	37.0	37.0
48-49	36.2435	37.0	37.0	37.0	37.0	37.0
50-51	36.2935	37.0	37.0	37.0	37.0	37.0
52-53	36.30575	37.0	37.0	37.0	37.0	37.0
54-55	36.21875	37.0	37.0	37.0	37.0	37.0
56-57	36.235749999999996	37.0	37.0	37.0	37.0	37.0
58-59	36.307500000000005	37.0	37.0	37.0	37.0	37.0
60-61	36.14975	37.0	37.0	37.0	37.0	37.0
62-63	36.230000000000004	37.0	37.0	37.0	37.0	37.0
64-65	36.150499999999994	37.0	37.0	37.0	37.0	37.0
66-67	36.129999999999995	37.0	37.0	37.0	37.0	37.0
68-69	36.086749999999995	37.0	37.0	37.0	37.0	37.0
70-71	36.078	37.0	37.0	37.0	37.0	37.0
72-73	36.085750000000004	37.0	37.0	37.0	37.0	37.0
74-75	36.06725	37.0	37.0	37.0	37.0	37.0
76-77	36.047	37.0	37.0	37.0	37.0	37.0
78-79	35.9845	37.0	37.0	37.0	37.0	37.0
80-81	36.0815	37.0	37.0	37.0	37.0	37.0
82-83	36.07775	37.0	37.0	37.0	37.0	37.0
84-85	36.06075	37.0	37.0	37.0	37.0	37.0
86-87	36.0235	37.0	37.0	37.0	37.0	37.0
88-89	35.8475	37.0	37.0	37.0	37.0	37.0
90-91	35.876999999999995	37.0	37.0	37.0	37.0	37.0
92-93	35.935500000000005	37.0	37.0	37.0	37.0	37.0
94-95	36.03475	37.0	37.0	37.0	37.0	37.0
96-97	35.90775	37.0	37.0	37.0	37.0	37.0
98-99	35.877750000000006	37.0	37.0	37.0	37.0	37.0
100-101	35.57875	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	0.0
25	3.0
26	6.0
27	11.0
28	5.0
29	13.0
30	14.0
31	23.0
32	22.0
33	76.0
34	150.0
35	429.0
36	2785.0
37	461.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.88176352705411	18.161322645290582	12.825651302605209	28.1312625250501
2	21.8	25.900000000000002	33.825	18.475
3	19.875	28.599999999999998	30.65	20.875
4	24.825	33.725	21.15	20.3
5	26.6	31.974999999999998	22.5	18.925
6	21.95	34.725	23.549999999999997	19.775000000000002
7	21.575	21.349999999999998	38.125	18.95
8	25.224999999999998	21.9	27.275	25.6
9	22.625	23.5	28.575	25.3
10-11	24.2375	31.125000000000004	22.775000000000002	21.8625
12-13	25.637500000000003	25.2375	25.2875	23.8375
14-15	25.087500000000002	25.7875	27.8625	21.2625
16-17	24.099999999999998	27.275	28.4375	20.1875
18-19	24.125	27.474999999999998	27.35	21.05
20-21	23.7625	28.275	27.250000000000004	20.7125
22-23	23.4125	26.974999999999998	27.250000000000004	22.3625
24-25	23.8875	29.6375	25.8	20.674999999999997
26-27	24.3125	27.950000000000003	26.25	21.4875
28-29	24.3625	26.575	27.487499999999997	21.575
30-31	24.025	27.487499999999997	26.487500000000004	22.0
32-33	23.8625	26.924999999999997	28.1875	21.025
34-35	24.775	26.2625	25.7625	23.200000000000003
36-37	23.6375	26.950000000000003	28.499999999999996	20.9125
38-39	23.5	26.474999999999998	26.900000000000002	23.125
40-41	22.0875	26.8125	27.775	23.325000000000003
42-43	23.375	28.9375	26.0625	21.625
44-45	23.35	27.462500000000002	29.049999999999997	20.1375
46-47	24.8125	28.1125	24.6125	22.4625
48-49	23.2125	26.737499999999997	29.1375	20.9125
50-51	23.5	27.975	25.474999999999998	23.05
52-53	24.474999999999998	27.8875	27.3125	20.325
54-55	25.525	28.0875	25.2875	21.099999999999998
56-57	21.6875	29.0875	26.150000000000002	23.075000000000003
58-59	22.325	28.725	25.9625	22.9875
60-61	21.7375	28.125	26.737499999999997	23.400000000000002
62-63	23.6125	27.712500000000002	25.637500000000003	23.0375
64-65	23.5125	28.000000000000004	28.1875	20.3
66-67	21.525	27.05	27.400000000000002	24.025
68-69	23.2375	29.325000000000003	26.174999999999997	21.2625
70-71	22.9375	28.962500000000002	26.9125	21.1875
72-73	21.65	29.275000000000002	26.0125	23.0625
74-75	23.8625	27.575	27.1625	21.4
76-77	23.575	28.5625	25.374999999999996	22.4875
78-79	24.2375	27.187499999999996	27.725	20.849999999999998
80-81	23.075000000000003	28.037499999999998	26.8125	22.075
82-83	23.0125	26.224999999999998	27.575	23.1875
84-85	21.3125	27.775	27.6375	23.275000000000002
86-87	22.975	29.2875	25.624999999999996	22.112499999999997
88-89	23.425	27.200000000000003	27.037499999999998	22.3375
90-91	22.725	28.299999999999997	26.375	22.6
92-93	23.525	28.3875	27.250000000000004	20.837500000000002
94-95	23.0375	30.5125	24.575	21.875
96-97	23.5	28.6625	25.8	22.037499999999998
98-99	22.5625	28.7375	26.0375	22.662499999999998
100-101	24.212500000000002	28.275	26.724999999999998	20.7875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.5
25	2.0
26	2.0
27	1.0
28	2.5
29	6.0
30	26.0
31	84.0
32	135.5
33	130.0
34	107.5
35	90.0
36	81.0
37	84.5
38	85.5
39	89.0
40	109.5
41	149.0
42	189.0
43	209.0
44	195.0
45	189.0
46	203.5
47	221.5
48	192.5
49	131.0
50	119.5
51	125.5
52	113.0
53	100.0
54	106.5
55	127.0
56	99.5
57	71.5
58	83.0
59	80.5
60	61.5
61	43.0
62	32.5
63	15.5
64	6.0
65	4.0
66	3.0
67	6.5
68	10.5
69	10.5
70	8.5
71	8.5
72	7.5
73	4.5
74	4.5
75	5.0
76	8.0
77	9.5
78	6.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.300000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.8686679174484	34.575
2	18.621013133208255	19.85
3	6.3320825515947465	10.125
4	3.564727954971857	7.6
5	1.876172607879925	5.0
6	1.4071294559099436	4.5
7	0.797373358348968	2.9749999999999996
8	0.37523452157598497	1.6
9	0.3283302063789869	1.575
>10	1.8292682926829267	12.2
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	26	0.65	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	24	0.6	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	19	0.475	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	18	0.44999999999999996	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	17	0.42500000000000004	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	17	0.42500000000000004	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	17	0.42500000000000004	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	14	0.35000000000000003	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	14	0.35000000000000003	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	13	0.325	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	13	0.325	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	13	0.325	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	12	0.3	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	12	0.3	No Hit
CTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTC	12	0.3	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	12	0.3	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	11	0.27499999999999997	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	11	0.27499999999999997	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	11	0.27499999999999997	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	11	0.27499999999999997	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	11	0.27499999999999997	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	10	0.25	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	10	0.25	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	10	0.25	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	10	0.25	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	10	0.25	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	10	0.25	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	10	0.25	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	10	0.25	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	10	0.25	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	10	0.25	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	10	0.25	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	10	0.25	No Hit
CTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCA	10	0.25	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	10	0.25	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	10	0.25	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	10	0.25	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	10	0.25	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	10	0.25	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	9	0.22499999999999998	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	9	0.22499999999999998	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	9	0.22499999999999998	No Hit
GGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGC	9	0.22499999999999998	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	9	0.22499999999999998	No Hit
GTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTG	9	0.22499999999999998	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	9	0.22499999999999998	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	8	0.2	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	8	0.2	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	8	0.2	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	8	0.2	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	8	0.2	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	8	0.2	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	8	0.2	No Hit
CTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGT	8	0.2	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	7	0.17500000000000002	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	7	0.17500000000000002	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	7	0.17500000000000002	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	7	0.17500000000000002	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	7	0.17500000000000002	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	7	0.17500000000000002	No Hit
GAAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGT	7	0.17500000000000002	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	7	0.17500000000000002	No Hit
CTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAA	7	0.17500000000000002	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	7	0.17500000000000002	No Hit
CGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTC	7	0.17500000000000002	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	7	0.17500000000000002	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	7	0.17500000000000002	No Hit
TATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTC	7	0.17500000000000002	No Hit
CGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTT	7	0.17500000000000002	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	7	0.17500000000000002	No Hit
CACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGT	7	0.17500000000000002	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	6	0.15	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	6	0.15	No Hit
CTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTG	6	0.15	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	6	0.15	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	6	0.15	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	6	0.15	No Hit
CCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTT	6	0.15	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	6	0.15	No Hit
CCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCT	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
GGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACAC	6	0.15	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	6	0.15	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	6	0.15	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	6	0.15	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	6	0.15	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	6	0.15	No Hit
GACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAA	6	0.15	No Hit
CAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAA	6	0.15	No Hit
GAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAA	6	0.15	No Hit
GTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAG	6	0.15	No Hit
ATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAA	6	0.15	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	6	0.15	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	6	0.15	No Hit
TTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGAC	6	0.15	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	6	0.15	No Hit
GCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTG	6	0.15	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	6	0.15	No Hit
GTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTA	6	0.15	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	6	0.15	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	6	0.15	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	5	0.125	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	5	0.125	No Hit
CCTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAA	5	0.125	No Hit
ATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACA	5	0.125	No Hit
AAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGC	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
CTTCCTTCCTTCCTTCCTTCCTTCCAGGAAGCCCCTGCTGCCTCCCCCTG	5	0.125	No Hit
CAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGACTCGGCGC	5	0.125	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	5	0.125	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	5	0.125	No Hit
GTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCT	5	0.125	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	5	0.125	No Hit
TGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATT	5	0.125	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	5	0.125	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	5	0.125	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	5	0.125	No Hit
TCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGT	5	0.125	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	5	0.125	No Hit
GGAAAAACCAGGAAGGGATGTTCTAGAACCCACACAAGCGCTGGCTGCCG	5	0.125	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	5	0.125	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	5	0.125	No Hit
TGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCT	5	0.125	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	5	0.125	No Hit
GAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGC	5	0.125	No Hit
ATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGT	5	0.125	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	5	0.125	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	5	0.125	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	5	0.125	No Hit
AGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAA	5	0.125	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	5	0.125	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	5	0.125	No Hit
GTCGGTGGTGACTGGCGGCGGTGAGGCGGCCGGTGGCGGCGGCGGCGGCG	5	0.125	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	5	0.125	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	5	0.125	No Hit
GAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTG	5	0.125	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	5	0.125	No Hit
AGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGA	5	0.125	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	5	0.125	No Hit
GCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCAT	5	0.125	No Hit
GCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.037500000000000006	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.1375	0.0	0.0	0.0	0.0
76-77	0.1875	0.0	0.0	0.0	0.0
78-79	0.3625	0.0	0.0	0.0	0.0
80-81	0.4625	0.0	0.0	0.0	0.0
82-83	0.5875	0.0	0.0	0.0	0.0
84-85	0.7124999999999999	0.0	0.0	0.0	0.0
86-87	0.875	0.0	0.0	0.0	0.0
88-89	1.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAATTC	35	0.00791152	27.142855	28-29
>>END_MODULE
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
Read 1771519 spots for SRR24882408.sra
Written 1771519 spots for SRR24882408.sra
Read 1771514 spots for SRR24882408.sra
Written 1771514 spots for SRR24882408.sra
SRR ids: ['SRR24882408.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__1wzaw88
SRR24882408.sra spots: 28344229
blocks: [[1, 1771514], [1771515, 3543028], [3543029, 5314542], [5314543, 7086056], [7086057, 8857570], [8857571, 10629084], [10629085, 12400598], [12400599, 14172112], [14172113, 15943626], [15943627, 17715140], [17715141, 19486654], [19486655, 21258168], [21258169, 23029682], [23029683, 24801196], [24801197, 26572710], [26572711, 28344229]]
SRR24882408 file size 975791
SRR24882408 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24882408 SRR24882408_1.fastq SRR24882408_2.fastq
Input file:	SRR24882408_1.fastq
Paired file:	SRR24882408_2.fastq
trimmed:	SRR24882408-trimmed-pair1.fastq, SRR24882408-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 05:30:03 2026 >> started

Fri Jul  3 05:30:55 2026 >> done (51.985s)
28344229 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       3 ( 0.00%) empty read pairs filtered out after trimming by size control
28344226 (100.00%) read pairs available; of these:
 1023726 ( 3.61%) trimmed read pairs available after processing
27320500 (96.39%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       3	  0.00%
 66	      60	  0.00%
 67	    1027	  0.00%
 68	    6157	  0.02%
 69	    7738	  0.03%
 70	    8853	  0.03%
 71	   10129	  0.04%
 72	   11255	  0.04%
 73	   11886	  0.04%
 74	   13010	  0.05%
 75	   14358	  0.05%
 76	   15254	  0.05%
 77	   16997	  0.06%
 78	   17945	  0.06%
 79	   19231	  0.07%
 80	   20490	  0.07%
 81	   22216	  0.08%
 82	   23872	  0.08%
 83	   25356	  0.09%
 84	   27354	  0.10%
 85	   29059	  0.10%
 86	   29907	  0.11%
 87	   32657	  0.12%
 88	   35321	  0.12%
 89	   35733	  0.13%
 90	   38615	  0.14%
 91	   41415	  0.15%
 92	   43405	  0.15%
 93	   46176	  0.16%
 94	   48521	  0.17%
 95	   50339	  0.18%
 96	   52043	  0.18%
 97	   57700	  0.20%
 98	   58506	  0.21%
 99	   63924	  0.23%
100	   87214	  0.31%
101	27320500	 96.39%
28344226 reads passed initial QC


criterion=sequence-density
sequence-density=0.89
sequence-density-rank=1
fanout-score=1.81
fanout-score-rank=34
prefix-density=1.62
prefix-fanout=1.0
sequence=GCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAGGGCACCGGGCAGGTCATCGAGGTGGCCTGCAGCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCATATTCAGCACCATGGCCACCAATCTTCCCCCAGGCAGCCTTGATGTTGCTTTTGTCTTCCCCAGAGAGCACCATGGTTTCTTCCTGAGTCTGTCA


criterion=fanout-score
sequence-density=0.13
sequence-density-rank=16
fanout-score=29.16
fanout-score-rank=1
prefix-density=3.34
prefix-fanout=1.1
sequence=GGTGGTGGGGACGCTAGCAAACA


criterion=sequence-density
sequence-density=0.60
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=34
prefix-density=0.61
prefix-fanout=2.0
sequence=AAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=27
fanout-score=82.04
fanout-score-rank=1
prefix-density=0.40
prefix-fanout=4.1
sequence=CCTGCCCCTCCCAAAGCCGAAGCCAAAGCGAAGGCCTTGAAAGCTAAGAAGGCGGTGCTGAAAGGCGTCCACAGCCACAAAAAGAAGAAGATCCGAACGTCACCCACCTTCCGGCGGCCCAAGACCCTGCGGCTCCGGAGGCAGCCAAAATATCCTCGGAAGAGTGCGCCCAGGAGAAACAAGCTTGACCACTATGCTATCATCAAATTCCCACTGACCACGGAGTCAGCCATGAAGAAAATAGAGGACAACAACACTCTTGTGTTCATTGTGGATGTCAAGGCCAATAAGCATCAGATCAAACAGGCTGTCAAGAAACTCTATGACATCGATGTGGCCAAAGTCAATACCCTGATAAGGCCCGACGGAGAGAAGAAGGCGTATGTTCGCTTGGCTCCTGATTATGATGCCCTAGATGTTGCCAACAAGATTGGGATCATCTAAACTGAGTCC
SRR24882408 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 05:31:36
                             Started mapping on |	Jul 03 05:31:37
                                    Finished on |	Jul 03 05:34:29
       Mapping speed, Million of reads per hour |	593.25

                          Number of input reads |	28344226
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	22824736
                        Uniquely mapped reads % |	80.53%
                          Average mapped length |	200.32
                       Number of splices: Total |	12802352
            Number of splices: Annotated (sjdb) |	12707230
                       Number of splices: GT/AG |	12681969
                       Number of splices: GC/AG |	96552
                       Number of splices: AT/AC |	8790
               Number of splices: Non-canonical |	15041
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.06
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.51
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5509930
             % of reads mapped to multiple loci |	19.44%
        Number of reads mapped to too many loci |	5079
             % of reads mapped to too many loci |	0.02%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9560	9560	9560
N_multimapping	5509930	5509930	5509930
N_noFeature	1639276	20779226	2443415
N_ambiguous	1536372	583471	273383
UnstrandedReadsAssigned:19649088 PositiveStrandReadsAssigned:1462039 NegativeStrandReadsAssigned:20107938
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882408 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882408-trimmed-pair1.fastq
                             SRR24882408-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,344,226 reads, 23,938,725 reads pseudoaligned
[quant] estimated average fragment length: 181.991
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,293 rounds

 109283 SRR24882408.ke.tsv
  52636 SRR24882408.se.tsv
 161919 total
==> SRR24882408.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR24882408.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	7
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882408 completed mapping pipeline successfully
