Starting /dee2/code/volunteer_pipeline.sh SRR24882414
    current disk space = 1396901048
    free memory = 197898144 
SRR24882414 SRAfilesize
4978c98608ea6a8ca2c919a87f952069  SRR24882414.sra
SRR24882414.sra file validated
SRR24882414 is paired end
SRR24882414 is conventional basespace
SRR24882414 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.61075	37.0	37.0	37.0	37.0	37.0
2	36.5095	37.0	37.0	37.0	37.0	37.0
3	36.6445	37.0	37.0	37.0	37.0	37.0
4	36.5735	37.0	37.0	37.0	37.0	37.0
5	36.6465	37.0	37.0	37.0	37.0	37.0
6	36.701	37.0	37.0	37.0	37.0	37.0
7	36.5935	37.0	37.0	37.0	37.0	37.0
8	36.6285	37.0	37.0	37.0	37.0	37.0
9	36.651	37.0	37.0	37.0	37.0	37.0
10-11	36.65075	37.0	37.0	37.0	37.0	37.0
12-13	36.65975	37.0	37.0	37.0	37.0	37.0
14-15	36.573	37.0	37.0	37.0	37.0	37.0
16-17	36.61	37.0	37.0	37.0	37.0	37.0
18-19	36.66375	37.0	37.0	37.0	37.0	37.0
20-21	36.535250000000005	37.0	37.0	37.0	37.0	37.0
22-23	36.578500000000005	37.0	37.0	37.0	37.0	37.0
24-25	36.55	37.0	37.0	37.0	37.0	37.0
26-27	36.524	37.0	37.0	37.0	37.0	37.0
28-29	36.602999999999994	37.0	37.0	37.0	37.0	37.0
30-31	36.4955	37.0	37.0	37.0	37.0	37.0
32-33	36.56725	37.0	37.0	37.0	37.0	37.0
34-35	36.50775	37.0	37.0	37.0	37.0	37.0
36-37	36.437749999999994	37.0	37.0	37.0	37.0	37.0
38-39	36.491	37.0	37.0	37.0	37.0	37.0
40-41	36.48475	37.0	37.0	37.0	37.0	37.0
42-43	36.482	37.0	37.0	37.0	37.0	37.0
44-45	36.4985	37.0	37.0	37.0	37.0	37.0
46-47	36.48825	37.0	37.0	37.0	37.0	37.0
48-49	36.373000000000005	37.0	37.0	37.0	37.0	37.0
50-51	36.438500000000005	37.0	37.0	37.0	37.0	37.0
52-53	36.485	37.0	37.0	37.0	37.0	37.0
54-55	36.48775	37.0	37.0	37.0	37.0	37.0
56-57	36.391625000000005	37.0	37.0	37.0	37.0	37.0
58-59	36.544	37.0	37.0	37.0	37.0	37.0
60-61	36.36475	37.0	37.0	37.0	37.0	37.0
62-63	36.42725	37.0	37.0	37.0	37.0	37.0
64-65	36.3285	37.0	37.0	37.0	37.0	37.0
66-67	36.40675	37.0	37.0	37.0	37.0	37.0
68-69	36.320750000000004	37.0	37.0	37.0	37.0	37.0
70-71	36.32625	37.0	37.0	37.0	37.0	37.0
72-73	36.3635	37.0	37.0	37.0	37.0	37.0
74-75	36.29975	37.0	37.0	37.0	37.0	37.0
76-77	36.29625	37.0	37.0	37.0	37.0	37.0
78-79	36.27875	37.0	37.0	37.0	37.0	37.0
80-81	36.267250000000004	37.0	37.0	37.0	37.0	37.0
82-83	36.28175	37.0	37.0	37.0	37.0	37.0
84-85	36.27575	37.0	37.0	37.0	37.0	37.0
86-87	36.3495	37.0	37.0	37.0	37.0	37.0
88-89	36.2125	37.0	37.0	37.0	37.0	37.0
90-91	36.2355	37.0	37.0	37.0	37.0	37.0
92-93	36.164	37.0	37.0	37.0	37.0	37.0
94-95	36.13975	37.0	37.0	37.0	37.0	37.0
96-97	36.1025	37.0	37.0	37.0	37.0	37.0
98-99	36.12225	37.0	37.0	37.0	37.0	37.0
100-101	35.956	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	2.0
27	5.0
28	7.0
29	21.0
30	20.0
31	21.0
32	24.0
33	41.0
34	81.0
35	237.0
36	2787.0
37	752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.23430857714429	10.677669417354338	12.07801950487622	40.010002500625156
2	19.125	16.400000000000002	36.449999999999996	28.025
3	18.76876876876877	21.146146146146148	29.929929929929934	30.155155155155157
4	24.75	28.7	23.400000000000002	23.150000000000002
5	26.700000000000003	30.599999999999998	24.975	17.724999999999998
6	21.4	35.875	25.95	16.775000000000002
7	16.85	24.474999999999998	43.275000000000006	15.4
8	22.125	24.0	29.975	23.9
9	21.475	24.224999999999998	31.624999999999996	22.675
10-11	21.2	35.175	23.225	20.4
12-13	22.4375	26.125	28.95	22.4875
14-15	20.75	30.075000000000003	28.6625	20.5125
16-17	21.2375	28.787499999999998	26.1125	23.8625
18-19	23.5625	29.7125	23.875	22.85
20-21	22.525000000000002	28.425	27.375	21.675
22-23	23.225	27.700000000000003	26.737499999999997	22.3375
24-25	23.7	28.799999999999997	25.8	21.7
26-27	22.787499999999998	30.162499999999998	25.95	21.099999999999998
28-29	22.1875	30.312499999999996	26.575	20.925
30-31	21.575	27.05	29.175	22.2
32-33	21.6125	25.8625	30.15	22.375
34-35	18.787499999999998	29.725	26.8125	24.675
36-37	21.9	28.7375	29.349999999999998	20.0125
38-39	21.087500000000002	28.262500000000003	29.7375	20.9125
40-41	20.150000000000002	30.587500000000002	29.3875	19.875
42-43	21.925	30.9375	26.5125	20.625
44-45	19.3	31.337500000000002	26.450000000000003	22.912499999999998
46-47	23.474999999999998	32.5	22.5625	21.462500000000002
48-49	22.125	32.1125	24.4375	21.325
50-51	22.1875	27.650000000000002	26.775	23.3875
52-53	21.725	26.6625	28.1875	23.425
54-55	20.225	28.15	27.675	23.95
56-57	18.86485810726341	30.01625203150394	28.51606450806351	22.602825353169145
58-59	22.037499999999998	25.837500000000002	29.4	22.725
60-61	20.325	31.2625	25.974999999999998	22.4375
62-63	24.275	27.1	26.0625	22.5625
64-65	22.675	26.387500000000003	27.8875	23.05
66-67	24.1875	26.75	28.675	20.3875
68-69	22.162499999999998	28.549999999999997	27.125	22.162499999999998
70-71	22.825	28.425	26.650000000000002	22.1
72-73	21.0375	31.412499999999998	26.625	20.925
74-75	21.5375	28.65	29.1875	20.625
76-77	24.712500000000002	27.900000000000002	25.5625	21.825
78-79	24.05	27.8875	27.875	20.1875
80-81	25.874999999999996	28.375	26.0625	19.6875
82-83	23.525	28.425	27.775	20.275000000000002
84-85	20.4	29.5375	25.05	25.0125
86-87	23.925	27.737499999999997	27.537499999999998	20.8
88-89	25.7125	26.900000000000002	28.8375	18.55
90-91	22.4375	26.275	29.262500000000003	22.025
92-93	21.975	28.499999999999996	27.150000000000002	22.375
94-95	21.85	29.549999999999997	27.4125	21.1875
96-97	21.775	28.9875	23.7625	25.474999999999998
98-99	22.168042010502624	29.332333083270818	27.70692673168292	20.792698174543638
100-101	23.0375	28.65	27.224999999999998	21.087500000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.5
17	1.0
18	1.5
19	1.0
20	0.0
21	1.5
22	3.0
23	3.5
24	3.0
25	14.5
26	26.0
27	14.5
28	5.5
29	8.0
30	28.5
31	54.5
32	96.0
33	126.5
34	119.5
35	143.0
36	143.0
37	109.5
38	75.0
39	64.5
40	94.0
41	188.0
42	267.0
43	217.0
44	156.0
45	164.5
46	230.0
47	258.0
48	197.5
49	142.0
50	129.5
51	108.0
52	87.0
53	76.0
54	59.0
55	69.0
56	70.0
57	49.5
58	70.0
59	107.0
60	83.0
61	34.5
62	26.0
63	19.0
64	5.0
65	3.0
66	4.0
67	5.5
68	6.0
69	6.5
70	4.0
71	0.0
72	2.0
73	2.5
74	2.5
75	3.5
76	2.5
77	2.5
78	3.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.1
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0125
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.025
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.32243871778756	22.8
2	17.724701445631677	14.099999999999998
3	8.799497171590195	10.5
4	4.902576995600252	7.8
5	2.8912633563796355	5.75
6	1.6341923318667504	3.9
7	1.508485229415462	4.2
8	0.6913890634820867	2.1999999999999997
9	0.502828409805154	1.7999999999999998
>10	4.022627278441232	26.950000000000003
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	48	1.2	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	44	1.0999999999999999	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	35	0.8750000000000001	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	30	0.75	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	29	0.7250000000000001	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	27	0.675	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	26	0.65	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	26	0.65	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	25	0.625	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	23	0.575	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	23	0.575	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	21	0.525	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	20	0.5	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	20	0.5	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	20	0.5	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	19	0.475	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	19	0.475	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	19	0.475	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	19	0.475	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	18	0.44999999999999996	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	18	0.44999999999999996	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	18	0.44999999999999996	No Hit
CTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCA	17	0.42500000000000004	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	17	0.42500000000000004	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	17	0.42500000000000004	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	16	0.4	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	16	0.4	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	16	0.4	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	15	0.375	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	15	0.375	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	15	0.375	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	14	0.35000000000000003	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	14	0.35000000000000003	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	14	0.35000000000000003	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	14	0.35000000000000003	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	14	0.35000000000000003	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	14	0.35000000000000003	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	14	0.35000000000000003	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	13	0.325	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	13	0.325	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	13	0.325	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	13	0.325	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	13	0.325	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	13	0.325	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	12	0.3	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	12	0.3	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	11	0.27499999999999997	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	11	0.27499999999999997	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	11	0.27499999999999997	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	11	0.27499999999999997	No Hit
GTCCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATA	11	0.27499999999999997	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	11	0.27499999999999997	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	11	0.27499999999999997	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	10	0.25	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	10	0.25	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	10	0.25	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	10	0.25	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	10	0.25	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	10	0.25	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	10	0.25	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	10	0.25	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	10	0.25	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	10	0.25	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	10	0.25	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	9	0.22499999999999998	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	9	0.22499999999999998	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	9	0.22499999999999998	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	9	0.22499999999999998	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	9	0.22499999999999998	No Hit
TGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACAT	9	0.22499999999999998	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	9	0.22499999999999998	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	9	0.22499999999999998	No Hit
GACAGGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAG	8	0.2	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	8	0.2	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	8	0.2	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	8	0.2	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	8	0.2	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	8	0.2	No Hit
ATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTC	8	0.2	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	8	0.2	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	8	0.2	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	8	0.2	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	8	0.2	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	7	0.17500000000000002	No Hit
GTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACAC	7	0.17500000000000002	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	7	0.17500000000000002	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	7	0.17500000000000002	No Hit
CCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGT	7	0.17500000000000002	No Hit
GGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTAC	7	0.17500000000000002	No Hit
ATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCT	7	0.17500000000000002	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	7	0.17500000000000002	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	7	0.17500000000000002	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	7	0.17500000000000002	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	7	0.17500000000000002	No Hit
GCTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAA	7	0.17500000000000002	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	7	0.17500000000000002	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	7	0.17500000000000002	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	7	0.17500000000000002	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	7	0.17500000000000002	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	7	0.17500000000000002	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	7	0.17500000000000002	No Hit
GCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTT	7	0.17500000000000002	No Hit
GTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATA	7	0.17500000000000002	No Hit
GTCTACCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAA	7	0.17500000000000002	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	7	0.17500000000000002	No Hit
AGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGC	7	0.17500000000000002	No Hit
GAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGT	7	0.17500000000000002	No Hit
CTACCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGA	6	0.15	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	6	0.15	No Hit
TTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACT	6	0.15	No Hit
CTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGATCCATTCT	6	0.15	No Hit
CCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGC	6	0.15	No Hit
GTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGC	6	0.15	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	6	0.15	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	6	0.15	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	6	0.15	No Hit
CAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGAT	6	0.15	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	6	0.15	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	6	0.15	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	6	0.15	No Hit
CTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTA	6	0.15	No Hit
GAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACATCCC	6	0.15	No Hit
GTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGGA	6	0.15	No Hit
GTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTG	6	0.15	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	6	0.15	No Hit
TTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTT	6	0.15	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	6	0.15	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	6	0.15	No Hit
TCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAAT	6	0.15	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	6	0.15	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	6	0.15	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	6	0.15	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	6	0.15	No Hit
CAGAGAACATGTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTG	5	0.125	No Hit
GCCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAA	5	0.125	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	5	0.125	No Hit
GGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATT	5	0.125	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	5	0.125	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	5	0.125	No Hit
GGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGC	5	0.125	No Hit
TCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAA	5	0.125	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	5	0.125	No Hit
ATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTCACGGC	5	0.125	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	5	0.125	No Hit
GGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTT	5	0.125	No Hit
GGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGC	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	5	0.125	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	5	0.125	No Hit
CTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAGAAA	5	0.125	No Hit
CGTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGAT	5	0.125	No Hit
ATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTC	5	0.125	No Hit
CGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGG	5	0.125	No Hit
GCCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	5	0.125	No Hit
TTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTG	5	0.125	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	5	0.125	No Hit
CTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGT	5	0.125	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	5	0.125	No Hit
GGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCA	5	0.125	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	5	0.125	No Hit
CCCCGACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	5	0.125	No Hit
GGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG	5	0.125	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	5	0.125	No Hit
ACACATATGCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTC	5	0.125	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	5	0.125	No Hit
TAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACA	5	0.125	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	5	0.125	No Hit
TTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCC	5	0.125	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	5	0.125	No Hit
GAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATT	5	0.125	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	5	0.125	No Hit
CCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTGGC	5	0.125	No Hit
CCTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCC	5	0.125	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	5	0.125	No Hit
AGAGAACATGTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGT	5	0.125	No Hit
TAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAAC	5	0.125	No Hit
CCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACA	5	0.125	No Hit
AGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTT	5	0.125	No Hit
CTGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTA	5	0.125	No Hit
GCCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.025	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.125	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.175	0.0	0.0	0.0	0.0
80-81	0.225	0.0	0.0	0.0	0.0
82-83	0.35	0.0	0.0	0.0	0.0
84-85	0.6125	0.0	0.0	0.0	0.0
86-87	0.7875	0.0	0.0	0.0	0.0
88-89	0.875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882414 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882414_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.30075	37.0	37.0	37.0	37.0	37.0
2	36.371	37.0	37.0	37.0	37.0	37.0
3	36.474	37.0	37.0	37.0	37.0	37.0
4	36.38	37.0	37.0	37.0	37.0	37.0
5	36.513	37.0	37.0	37.0	37.0	37.0
6	36.3025	37.0	37.0	37.0	37.0	37.0
7	36.35975	37.0	37.0	37.0	37.0	37.0
8	36.546	37.0	37.0	37.0	37.0	37.0
9	36.5065	37.0	37.0	37.0	37.0	37.0
10-11	36.45875	37.0	37.0	37.0	37.0	37.0
12-13	36.472	37.0	37.0	37.0	37.0	37.0
14-15	36.417	37.0	37.0	37.0	37.0	37.0
16-17	36.38675	37.0	37.0	37.0	37.0	37.0
18-19	36.39125	37.0	37.0	37.0	37.0	37.0
20-21	36.42	37.0	37.0	37.0	37.0	37.0
22-23	36.37475	37.0	37.0	37.0	37.0	37.0
24-25	36.342	37.0	37.0	37.0	37.0	37.0
26-27	36.312250000000006	37.0	37.0	37.0	37.0	37.0
28-29	36.29	37.0	37.0	37.0	37.0	37.0
30-31	36.30525	37.0	37.0	37.0	37.0	37.0
32-33	36.260000000000005	37.0	37.0	37.0	37.0	37.0
34-35	36.329	37.0	37.0	37.0	37.0	37.0
36-37	36.301500000000004	37.0	37.0	37.0	37.0	37.0
38-39	36.28575	37.0	37.0	37.0	37.0	37.0
40-41	36.273250000000004	37.0	37.0	37.0	37.0	37.0
42-43	36.263	37.0	37.0	37.0	37.0	37.0
44-45	36.19725	37.0	37.0	37.0	37.0	37.0
46-47	36.13425	37.0	37.0	37.0	37.0	37.0
48-49	36.14175	37.0	37.0	37.0	37.0	37.0
50-51	36.08175	37.0	37.0	37.0	37.0	37.0
52-53	36.1735	37.0	37.0	37.0	37.0	37.0
54-55	36.15075	37.0	37.0	37.0	37.0	37.0
56-57	36.12425	37.0	37.0	37.0	37.0	37.0
58-59	36.082499999999996	37.0	37.0	37.0	37.0	37.0
60-61	36.05875	37.0	37.0	37.0	37.0	37.0
62-63	35.999	37.0	37.0	37.0	37.0	37.0
64-65	36.051249999999996	37.0	37.0	37.0	37.0	37.0
66-67	36.02475	37.0	37.0	37.0	37.0	37.0
68-69	35.964749999999995	37.0	37.0	37.0	37.0	37.0
70-71	35.88275	37.0	37.0	37.0	37.0	37.0
72-73	35.938	37.0	37.0	37.0	37.0	37.0
74-75	35.980500000000006	37.0	37.0	37.0	37.0	37.0
76-77	35.991	37.0	37.0	37.0	37.0	37.0
78-79	35.893	37.0	37.0	37.0	37.0	37.0
80-81	35.87775	37.0	37.0	37.0	37.0	37.0
82-83	35.88975	37.0	37.0	37.0	37.0	37.0
84-85	35.861000000000004	37.0	37.0	37.0	37.0	37.0
86-87	35.94325	37.0	37.0	37.0	37.0	37.0
88-89	35.814750000000004	37.0	37.0	37.0	37.0	37.0
90-91	35.771	37.0	37.0	37.0	37.0	37.0
92-93	35.829625	37.0	37.0	37.0	37.0	37.0
94-95	35.7505	37.0	37.0	37.0	37.0	37.0
96-97	35.760999999999996	37.0	37.0	37.0	37.0	37.0
98-99	35.74425	37.0	37.0	37.0	37.0	37.0
100-101	35.498999999999995	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	4.0
25	1.0
26	6.0
27	8.0
28	14.0
29	13.0
30	21.0
31	32.0
32	44.0
33	78.0
34	141.0
35	542.0
36	2763.0
37	332.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.36356802806314	18.541718867451767	14.106740165372086	27.987972939113003
2	20.325	26.825	35.3	17.549999999999997
3	19.75	27.200000000000003	32.6	20.45
4	23.474999999999998	34.425	23.150000000000002	18.95
5	24.8	32.175	25.174999999999997	17.849999999999998
6	21.5	34.75	24.125	19.625
7	20.580145036259065	20.68017004251063	40.03500875218805	18.704676169042262
8	22.775000000000002	22.725	29.75	24.75
9	22.05	25.55	28.749999999999996	23.65
10-11	23.4625	31.974999999999998	23.325000000000003	21.2375
12-13	24.625	25.937500000000004	27.212500000000002	22.225
14-15	23.8125	25.674999999999997	29.812499999999996	20.7
16-17	24.4	27.725	30.099999999999998	17.775
18-19	23.400000000000002	27.5875	28.599999999999998	20.4125
20-21	22.6125	29.6375	28.449999999999996	19.3
22-23	21.6	29.975	27.075	21.349999999999998
24-25	24.25	28.3625	26.637499999999996	20.75
26-27	22.8375	27.3875	28.6625	21.1125
28-29	24.337500000000002	27.6625	29.1375	18.862499999999997
30-31	23.799999999999997	27.85	28.025	20.325
32-33	22.975	26.637499999999996	30.3	20.0875
34-35	23.075000000000003	26.724999999999998	27.750000000000004	22.45
36-37	22.675	27.150000000000002	29.7875	20.3875
38-39	22.95	27.125	28.975	20.95
40-41	22.3375	26.775	30.2	20.6875
42-43	22.575	28.3875	28.1875	20.849999999999998
44-45	20.925	28.6375	30.2125	20.225
46-47	23.225	27.875	26.575	22.325
48-49	22.175	27.825	29.5	20.5
50-51	21.9	27.450000000000003	27.55	23.1
52-53	22.45	27.35	30.125	20.075000000000003
54-55	23.8875	26.3625	28.075	21.675
56-57	19.925	29.4125	29.125	21.5375
58-59	20.355088772193046	28.51962990747687	28.707176794198553	22.418104526131533
60-61	20.3	28.237499999999997	28.3875	23.075000000000003
62-63	22.400000000000002	27.712500000000002	27.987499999999997	21.9
64-65	22.0	27.287499999999998	29.7875	20.925
66-67	20.5625	25.4875	30.112499999999997	23.8375
68-69	23.0375	28.4125	28.7	19.85
70-71	21.15	28.849999999999998	28.8625	21.1375
72-73	22.15	27.375	28.6625	21.8125
74-75	23.425	25.6	29.349999999999998	21.625
76-77	22.0625	27.037499999999998	28.65	22.25
78-79	22.162499999999998	26.825	30.125	20.8875
80-81	21.762500000000003	27.224999999999998	29.262500000000003	21.75
82-83	21.3625	26.6125	30.1875	21.837500000000002
84-85	20.275000000000002	26.387500000000003	31.0625	22.275
86-87	22.3	28.4375	27.250000000000004	22.0125
88-89	23.25	26.724999999999998	28.449999999999996	21.575
90-91	21.6125	27.575	27.474999999999998	23.3375
92-93	22.290286285785722	29.141142642830353	28.028503562945367	20.540067508438558
94-95	21.65	30.3	26.625	21.425
96-97	22.075	27.275	28.1125	22.537499999999998
98-99	21.8625	28.725	27.8375	21.575
100-101	22.7375	26.974999999999998	28.287499999999998	22.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	2.0
19	1.5
20	0.0
21	0.0
22	0.0
23	0.5
24	0.5
25	0.5
26	2.0
27	2.0
28	4.0
29	12.5
30	43.0
31	100.0
32	150.5
33	138.0
34	114.0
35	103.5
36	87.5
37	108.0
38	108.0
39	100.5
40	135.0
41	190.5
42	247.5
43	230.0
44	228.5
45	256.5
46	248.5
47	232.0
48	178.0
49	120.5
50	106.5
51	103.5
52	74.5
53	52.5
54	61.5
55	100.5
56	80.0
57	31.5
58	33.0
59	38.5
60	29.0
61	18.0
62	19.5
63	14.0
64	5.5
65	3.0
66	1.5
67	2.5
68	2.5
69	5.5
70	7.0
71	4.0
72	2.5
73	4.0
74	10.5
75	10.0
76	8.5
77	14.0
78	8.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22499999999999998
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.025
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.16279069767442	24.15
2	17.790697674418603	15.299999999999999
3	9.244186046511627	11.924999999999999
4	5.63953488372093	9.700000000000001
5	3.372093023255814	7.249999999999999
6	2.3255813953488373	6.0
7	1.1627906976744187	3.5000000000000004
8	0.872093023255814	3.0
9	0.6395348837209303	2.475
>10	2.7906976744186047	16.7
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	40	1.0	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	36	0.8999999999999999	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	32	0.8	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	21	0.525	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	16	0.4	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	16	0.4	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	16	0.4	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	16	0.4	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	16	0.4	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	15	0.375	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	15	0.375	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	14	0.35000000000000003	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	14	0.35000000000000003	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	14	0.35000000000000003	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	14	0.35000000000000003	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	13	0.325	No Hit
CTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCAT	13	0.325	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	13	0.325	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	13	0.325	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	13	0.325	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	13	0.325	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	13	0.325	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	12	0.3	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	12	0.3	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	12	0.3	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	12	0.3	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	12	0.3	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	12	0.3	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	12	0.3	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	11	0.27499999999999997	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	11	0.27499999999999997	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	11	0.27499999999999997	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	11	0.27499999999999997	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	11	0.27499999999999997	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	11	0.27499999999999997	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	11	0.27499999999999997	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	11	0.27499999999999997	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	10	0.25	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	10	0.25	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	10	0.25	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	10	0.25	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	10	0.25	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	10	0.25	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	10	0.25	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	10	0.25	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	10	0.25	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	10	0.25	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	10	0.25	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	9	0.22499999999999998	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	9	0.22499999999999998	No Hit
TAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTAC	9	0.22499999999999998	No Hit
CAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAG	9	0.22499999999999998	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	9	0.22499999999999998	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	9	0.22499999999999998	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	9	0.22499999999999998	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	9	0.22499999999999998	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	9	0.22499999999999998	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	9	0.22499999999999998	No Hit
CAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAG	9	0.22499999999999998	No Hit
GCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGT	8	0.2	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	8	0.2	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	8	0.2	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	8	0.2	No Hit
GACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAA	8	0.2	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	8	0.2	No Hit
GTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGT	8	0.2	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	8	0.2	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	8	0.2	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	8	0.2	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	8	0.2	No Hit
GGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGG	8	0.2	No Hit
ACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTT	8	0.2	No Hit
CCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAAT	8	0.2	No Hit
CAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAAT	8	0.2	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	7	0.17500000000000002	No Hit
CCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTG	7	0.17500000000000002	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	7	0.17500000000000002	No Hit
CCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCC	7	0.17500000000000002	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	7	0.17500000000000002	No Hit
CTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTG	7	0.17500000000000002	No Hit
GGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACA	7	0.17500000000000002	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	7	0.17500000000000002	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	7	0.17500000000000002	No Hit
GATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCT	7	0.17500000000000002	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	7	0.17500000000000002	No Hit
CTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTC	7	0.17500000000000002	No Hit
AAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAA	7	0.17500000000000002	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	7	0.17500000000000002	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	7	0.17500000000000002	No Hit
GTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTT	7	0.17500000000000002	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	7	0.17500000000000002	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	7	0.17500000000000002	No Hit
GTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTG	7	0.17500000000000002	No Hit
CCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACC	7	0.17500000000000002	No Hit
GTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACA	6	0.15	No Hit
TACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCA	6	0.15	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	6	0.15	No Hit
GGGAAAGGTAACTCGGGCCCACGGAAACAGCGGTATGGTTCGTGCCAAAT	6	0.15	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	6	0.15	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	6	0.15	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	6	0.15	No Hit
ATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCT	6	0.15	No Hit
CCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTAC	6	0.15	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	6	0.15	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	6	0.15	No Hit
GCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCC	6	0.15	No Hit
GGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGA	6	0.15	No Hit
GGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTC	6	0.15	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	6	0.15	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	6	0.15	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	6	0.15	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	6	0.15	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	6	0.15	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	6	0.15	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	6	0.15	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	6	0.15	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	6	0.15	No Hit
CAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGT	6	0.15	No Hit
CTTGTATATATCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGT	6	0.15	No Hit
AAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACT	6	0.15	No Hit
CTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCC	6	0.15	No Hit
CCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACAT	6	0.15	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	6	0.15	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	6	0.15	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	6	0.15	No Hit
GTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAA	6	0.15	No Hit
AAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAG	6	0.15	No Hit
GCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAG	6	0.15	No Hit
GCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAA	6	0.15	No Hit
GATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATT	6	0.15	No Hit
CTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAG	6	0.15	No Hit
CAGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGT	6	0.15	No Hit
GGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTT	6	0.15	No Hit
AAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCC	6	0.15	No Hit
GAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAG	5	0.125	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	5	0.125	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	5	0.125	No Hit
CTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTG	5	0.125	No Hit
AAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAG	5	0.125	No Hit
CTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCT	5	0.125	No Hit
TTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTAC	5	0.125	No Hit
GTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACT	5	0.125	No Hit
CCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTT	5	0.125	No Hit
GTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTT	5	0.125	No Hit
AACCAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATG	5	0.125	No Hit
ATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCT	5	0.125	No Hit
TAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTT	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
ATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACT	5	0.125	No Hit
TGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACAC	5	0.125	No Hit
GGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTT	5	0.125	No Hit
CTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTC	5	0.125	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	5	0.125	No Hit
AACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTG	5	0.125	No Hit
GGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACAC	5	0.125	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	5	0.125	No Hit
GGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGC	5	0.125	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	5	0.125	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	5	0.125	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	5	0.125	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	5	0.125	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	5	0.125	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	5	0.125	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	5	0.125	No Hit
ATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCT	5	0.125	No Hit
GCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGAT	5	0.125	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	5	0.125	No Hit
AGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATC	5	0.125	No Hit
CCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAGCC	5	0.125	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	5	0.125	No Hit
ATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTG	5	0.125	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	5	0.125	No Hit
CAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAA	5	0.125	No Hit
CTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAA	5	0.125	No Hit
CCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACG	5	0.125	No Hit
AAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAA	5	0.125	No Hit
CCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGAC	5	0.125	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	5	0.125	No Hit
AAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAA	5	0.125	No Hit
AGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGT	5	0.125	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	5	0.125	No Hit
CAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATG	5	0.125	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	5	0.125	No Hit
CCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTAC	5	0.125	No Hit
CAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGA	5	0.125	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	5	0.125	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	5	0.125	No Hit
GCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGA	5	0.125	No Hit
GTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTC	5	0.125	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	5	0.125	No Hit
CATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGC	5	0.125	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.025	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.125	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.175	0.0	0.0	0.0	0.0
80-81	0.225	0.0	0.0	0.0	0.0
82-83	0.35	0.0	0.0	0.0	0.0
84-85	0.6125	0.0	0.0	0.0	0.0
86-87	0.7875	0.0	0.0	0.0	0.0
88-89	0.875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
Read 4924391 spots for SRR24882414.sra
Written 4924391 spots for SRR24882414.sra
Read 4924390 spots for SRR24882414.sra
Written 4924390 spots for SRR24882414.sra
SRR ids: ['SRR24882414.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ccdal5li
SRR24882414.sra spots: 39395121
blocks: [[1, 4924390], [4924391, 9848780], [9848781, 14773170], [14773171, 19697560], [19697561, 24621950], [24621951, 29546340], [29546341, 34470730], [34470731, 39395121]]
SRR24882414 file size 9519312
SRR24882414 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882414 SRR24882414_1.fastq SRR24882414_2.fastq
Input file:	SRR24882414_1.fastq
Paired file:	SRR24882414_2.fastq
trimmed:	SRR24882414-trimmed-pair1.fastq, SRR24882414-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 02:55:24 2026 >> started

Fri Jul  3 02:55:59 2026 >> done (34.979s)
39395121 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
39395121 (100.00%) read pairs available; of these:
  954900 ( 2.42%) trimmed read pairs available after processing
38440221 (97.58%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       3	  0.00%
 66	      82	  0.00%
 67	    1152	  0.00%
 68	    5999	  0.02%
 69	    7211	  0.02%
 70	    8206	  0.02%
 71	    9214	  0.02%
 72	   10375	  0.03%
 73	   11109	  0.03%
 74	   12080	  0.03%
 75	   13345	  0.03%
 76	   14291	  0.04%
 77	   15565	  0.04%
 78	   16710	  0.04%
 79	   17616	  0.04%
 80	   18899	  0.05%
 81	   20238	  0.05%
 82	   21713	  0.06%
 83	   22976	  0.06%
 84	   24492	  0.06%
 85	   25905	  0.07%
 86	   27265	  0.07%
 87	   29859	  0.08%
 88	   31965	  0.08%
 89	   32674	  0.08%
 90	   34742	  0.09%
 91	   37331	  0.09%
 92	   38793	  0.10%
 93	   41800	  0.11%
 94	   43989	  0.11%
 95	   45252	  0.11%
 96	   47861	  0.12%
 97	   51536	  0.13%
 98	   53661	  0.14%
 99	   59335	  0.15%
100	  101656	  0.26%
101	38440221	 97.58%
39395121 reads passed initial QC


criterion=sequence-density
sequence-density=0.60
sequence-density-rank=1
fanout-score=2.29
fanout-score-rank=29
prefix-density=0.64
prefix-fanout=2.2
sequence=GATTCTGCCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=69.43
fanout-score-rank=1
prefix-density=0.08
prefix-fanout=4.3
sequence=TTTTTTCCAAGGCATAAGAATAAGGTTTAATGCTGCACCCATCAAGGCAGCCCTAGTGTCTGTCAACATGGAAGCTCCCTGAGCGGTGCAGACCTCTCCAGTGTCCAGCTTCCTCAGTGTCTTCCACAGGACTACGCTGTGTCTGAGGCCTCGGCACATGGTACCATCGAGGCCTGTGGTGGGGGAGGGGCTGCCTAATCGTCCTTCTTAAACTTGCCGTTGATGTAAGGCACCCAGTCCAGGATCAGCCGCCAGTCCGTGGCCCACACCAGTCCCACGGCACCCACAGTGCCCCACATGCCGGCTGTGGGAATCCAGTTTCTGGCCAGTTCCCGGTAGCGCGGGCCTAGAAACCTGCTCAGCATCGCTGCAGGGTCTCAAT


criterion=sequence-density
sequence-density=0.23
sequence-density-rank=1
fanout-score=8.88
fanout-score-rank=12
prefix-density=0.78
prefix-fanout=2.7
sequence=TGGGCAGAATCCCTTCTGCTGTAGGCTACCAGCCCACCCTAGCCACCGACATGGGCACAATGCAGGAAAGGATCACCACCACCAAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=131.98
fanout-score-rank=1
prefix-density=0.23
prefix-fanout=3.4
sequence=GGGGAAACTTGTGAATGTAACTTGCCTGTGCCGTGTGACGGTCACGTGGGCCTGGTCTTTTGTGTGTGAGGCCCTTGACCGTGTGGCCTCTGCCTGGCTGTTTGGGGTCCTGCACGGCTTTCCCACCACCTGTAGCTCTTGTTGACCTGCCTGTTCACCTCATGAGTGAAGCGTCTGCCTGGCAGTGGGCCATGAACTGAGGGGTCTCTGTGTAGAGTAGAAGCTTCCTGTGCCTCCGGTTGCCAGGAGACAGCCTGTGCAGTTAAATGGACCTAGATTTTGT
SRR24882414 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 02:56:13
                             Started mapping on |	Jul 03 02:56:13
                                    Finished on |	Jul 03 02:58:16
       Mapping speed, Million of reads per hour |	1153.03

                          Number of input reads |	39395121
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	31178802
                        Uniquely mapped reads % |	79.14%
                          Average mapped length |	200.68
                       Number of splices: Total |	18588285
            Number of splices: Annotated (sjdb) |	18460960
                       Number of splices: GT/AG |	18413107
                       Number of splices: GC/AG |	143802
                       Number of splices: AT/AC |	12876
               Number of splices: Non-canonical |	18500
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.77
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.48
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	8201375
             % of reads mapped to multiple loci |	20.82%
        Number of reads mapped to too many loci |	7968
             % of reads mapped to too many loci |	0.02%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	14944	14944	14944
N_multimapping	8201375	8201375	8201375
N_noFeature	1757585	28986675	2504212
N_ambiguous	1890297	535704	414819
UnstrandedReadsAssigned:27530920 PositiveStrandReadsAssigned:1656423 NegativeStrandReadsAssigned:28259771
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882414 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882414-trimmed-pair1.fastq
                             SRR24882414-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 39,395,121 reads, 34,288,791 reads pseudoaligned
[quant] estimated average fragment length: 198.046
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,151 rounds

 109283 SRR24882414.ke.tsv
  52636 SRR24882414.se.tsv
 161919 total
==> SRR24882414.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	5.5	0	0
ENSMUST00000196221.1	9	3	0	0
ENSMUST00000179664.1	11	5	0	0
ENSMUST00000178537.1	12	6	0	0
ENSMUST00000178862.1	14	8	0	0
ENSMUST00000179520.1	11	5	0	0
ENSMUST00000179883.1	16	5.5	0	0
ENSMUST00000195858.1	10	4	0	0
ENSMUST00000179932.1	12	6	0	0

==> SRR24882414.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	20
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882414 completed mapping pipeline successfully
