Starting /dee2/code/volunteer_pipeline.sh SRR24882421
    current disk space = 1359120252
    free memory = 196084084 
SRR24882421 SRAfilesize
96e7c4d952dcee6c37fd1922c6c409e9  SRR24882421.sra
SRR24882421.sra file validated
SRR24882421 is paired end
SRR24882421 is conventional basespace
SRR24882421 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.523	37.0	37.0	37.0	37.0	37.0
2	36.55325	37.0	37.0	37.0	37.0	37.0
3	36.52475	37.0	37.0	37.0	37.0	37.0
4	36.5925	37.0	37.0	37.0	37.0	37.0
5	36.689	37.0	37.0	37.0	37.0	37.0
6	36.6625	37.0	37.0	37.0	37.0	37.0
7	36.578	37.0	37.0	37.0	37.0	37.0
8	36.5665	37.0	37.0	37.0	37.0	37.0
9	36.669	37.0	37.0	37.0	37.0	37.0
10-11	36.6375	37.0	37.0	37.0	37.0	37.0
12-13	36.6655	37.0	37.0	37.0	37.0	37.0
14-15	36.6275	37.0	37.0	37.0	37.0	37.0
16-17	36.6455	37.0	37.0	37.0	37.0	37.0
18-19	36.60225	37.0	37.0	37.0	37.0	37.0
20-21	36.667	37.0	37.0	37.0	37.0	37.0
22-23	36.56675	37.0	37.0	37.0	37.0	37.0
24-25	36.56	37.0	37.0	37.0	37.0	37.0
26-27	36.513999999999996	37.0	37.0	37.0	37.0	37.0
28-29	36.52575	37.0	37.0	37.0	37.0	37.0
30-31	36.56225	37.0	37.0	37.0	37.0	37.0
32-33	36.462500000000006	37.0	37.0	37.0	37.0	37.0
34-35	36.53175	37.0	37.0	37.0	37.0	37.0
36-37	36.50575	37.0	37.0	37.0	37.0	37.0
38-39	36.4995	37.0	37.0	37.0	37.0	37.0
40-41	36.50775	37.0	37.0	37.0	37.0	37.0
42-43	36.47975	37.0	37.0	37.0	37.0	37.0
44-45	36.44425	37.0	37.0	37.0	37.0	37.0
46-47	36.43275	37.0	37.0	37.0	37.0	37.0
48-49	36.434	37.0	37.0	37.0	37.0	37.0
50-51	36.4525	37.0	37.0	37.0	37.0	37.0
52-53	36.43575	37.0	37.0	37.0	37.0	37.0
54-55	36.462	37.0	37.0	37.0	37.0	37.0
56-57	36.4025	37.0	37.0	37.0	37.0	37.0
58-59	36.463	37.0	37.0	37.0	37.0	37.0
60-61	36.40775	37.0	37.0	37.0	37.0	37.0
62-63	36.45125	37.0	37.0	37.0	37.0	37.0
64-65	36.4415	37.0	37.0	37.0	37.0	37.0
66-67	36.38975000000001	37.0	37.0	37.0	37.0	37.0
68-69	36.34	37.0	37.0	37.0	37.0	37.0
70-71	36.323	37.0	37.0	37.0	37.0	37.0
72-73	36.39	37.0	37.0	37.0	37.0	37.0
74-75	36.21125	37.0	37.0	37.0	37.0	37.0
76-77	36.32	37.0	37.0	37.0	37.0	37.0
78-79	36.3335	37.0	37.0	37.0	37.0	37.0
80-81	36.294250000000005	37.0	37.0	37.0	37.0	37.0
82-83	36.228750000000005	37.0	37.0	37.0	37.0	37.0
84-85	36.25775	37.0	37.0	37.0	37.0	37.0
86-87	36.2055	37.0	37.0	37.0	37.0	37.0
88-89	36.18775	37.0	37.0	37.0	37.0	37.0
90-91	36.25175	37.0	37.0	37.0	37.0	37.0
92-93	36.26075	37.0	37.0	37.0	37.0	37.0
94-95	36.194	37.0	37.0	37.0	37.0	37.0
96-97	36.10625	37.0	37.0	37.0	37.0	37.0
98-99	36.09075	37.0	37.0	37.0	37.0	37.0
100-101	35.975	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	1.0
26	2.0
27	5.0
28	8.0
29	9.0
30	20.0
31	23.0
32	30.0
33	52.0
34	104.0
35	247.0
36	2641.0
37	857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.7	10.174999999999999	13.525	39.6
2	17.75443860965241	14.278569642410602	37.98449612403101	29.982495623905976
3	19.139354515886914	17.738303727795845	32.34926194645985	30.773079809857396
4	26.55	26.025	24.65	22.775000000000002
5	24.6	28.749999999999996	24.8	21.85
6	19.675	31.45	30.125	18.75
7	16.650000000000002	22.55	45.5	15.299999999999999
8	22.275	21.25	33.525	22.95
9	20.625	22.725	33.675	22.975
10-11	20.325	33.887499999999996	26.625	19.162499999999998
12-13	22.112499999999997	25.912499999999998	28.462500000000002	23.5125
14-15	20.1375	28.1	29.825000000000003	21.9375
16-17	21.025	27.025	26.35	25.6
18-19	22.675	30.0	26.1	21.224999999999998
20-21	23.2875	26.924999999999997	28.000000000000004	21.7875
22-23	20.7125	27.450000000000003	27.1375	24.7
24-25	24.1875	26.674999999999997	27.3625	21.775
26-27	20.45	30.062499999999996	28.025	21.462500000000002
28-29	20.1375	27.187499999999996	29.2	23.474999999999998
30-31	19.85	28.549999999999997	28.6125	22.9875
32-33	20.6375	26.974999999999998	29.65	22.7375
34-35	18.725	27.6	29.362500000000004	24.3125
36-37	22.3625	26.9125	30.45	20.275000000000002
38-39	19.8125	30.025000000000002	29.849999999999998	20.3125
40-41	20.8875	28.7375	29.8375	20.5375
42-43	20.5875	31.624999999999996	26.7625	21.025
44-45	18.725	31.874999999999996	27.125	22.275
46-47	21.575	29.65	24.5375	24.2375
48-49	19.9875	30.4875	26.9625	22.5625
50-51	22.650000000000002	25.662499999999998	28.3375	23.35
52-53	22.175	24.725	29.6875	23.4125
54-55	20.3	26.087500000000002	29.3375	24.275
56-57	18.4125	27.8625	29.975	23.75
58-59	20.4125	26.2625	29.762499999999996	23.5625
60-61	20.7875	27.8625	28.199999999999996	23.150000000000002
62-63	22.425	27.825	27.875	21.875
64-65	22.225	26.3	27.400000000000002	24.075
66-67	22.55	25.424999999999997	29.9375	22.0875
68-69	21.875	28.849999999999998	26.4625	22.8125
70-71	23.1125	28.825	25.4375	22.625
72-73	19.85	30.8	26.687499999999996	22.662499999999998
74-75	21.925	27.800000000000004	29.012500000000003	21.2625
76-77	24.3875	27.3875	26.8625	21.3625
78-79	22.475	25.7125	29.312500000000004	22.5
80-81	24.0	25.8125	28.5875	21.6
82-83	22.15	28.812500000000004	29.299999999999997	19.7375
84-85	20.175	29.7	27.35	22.775000000000002
86-87	22.025	29.7	26.950000000000003	21.325
88-89	23.8125	27.6125	28.0875	20.4875
90-91	21.4875	27.762500000000003	29.225	21.525
92-93	21.9625	26.724999999999998	29.575000000000003	21.7375
94-95	21.1625	30.025000000000002	26.637499999999996	22.175
96-97	20.200000000000003	29.025000000000002	23.575	27.200000000000003
98-99	19.892473118279568	29.994998749687422	28.119529882470616	21.99299824956239
100-101	22.75	28.5875	26.924999999999997	21.7375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	0.5
13	1.5
14	1.5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.0
24	0.5
25	3.5
26	6.5
27	5.5
28	8.0
29	23.5
30	66.0
31	97.0
32	130.0
33	143.5
34	135.0
35	159.0
36	133.5
37	105.5
38	78.5
39	51.5
40	92.5
41	161.0
42	212.0
43	171.5
44	111.5
45	134.5
46	231.5
47	282.0
48	218.5
49	152.0
50	142.5
51	135.0
52	104.0
53	81.5
54	63.5
55	68.5
56	69.5
57	56.5
58	77.0
59	92.0
60	64.5
61	27.5
62	19.0
63	18.5
64	7.5
65	2.0
66	3.0
67	9.0
68	6.5
69	1.0
70	1.0
71	1.5
72	5.0
73	6.0
74	7.0
75	5.5
76	0.5
77	0.5
78	1.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.075
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.025
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.737265415549594	18.925
2	21.983914209115284	16.400000000000002
3	9.316353887399465	10.424999999999999
4	4.825737265415549	7.199999999999999
5	2.8150134048257374	5.25
6	2.278820375335121	5.1
7	1.2734584450402144	3.325
8	1.2734584450402144	3.8
9	1.1394101876675604	3.8249999999999997
>10	4.3565683646112605	25.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	43	1.075	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	40	1.0	No Hit
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	38	0.95	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	31	0.775	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	28	0.7000000000000001	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	28	0.7000000000000001	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	27	0.675	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	27	0.675	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	23	0.575	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	22	0.5499999999999999	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	20	0.5	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	20	0.5	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	20	0.5	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	20	0.5	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	18	0.44999999999999996	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	18	0.44999999999999996	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	18	0.44999999999999996	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	18	0.44999999999999996	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	17	0.42500000000000004	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	16	0.4	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	16	0.4	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	15	0.375	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	15	0.375	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	15	0.375	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	14	0.35000000000000003	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	14	0.35000000000000003	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	14	0.35000000000000003	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	14	0.35000000000000003	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	14	0.35000000000000003	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	14	0.35000000000000003	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	14	0.35000000000000003	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	13	0.325	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	13	0.325	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	13	0.325	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	13	0.325	No Hit
CTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCAT	13	0.325	No Hit
GCCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGG	13	0.325	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	12	0.3	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	12	0.3	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	12	0.3	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	12	0.3	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	11	0.27499999999999997	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	11	0.27499999999999997	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	11	0.27499999999999997	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	11	0.27499999999999997	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	11	0.27499999999999997	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	11	0.27499999999999997	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	11	0.27499999999999997	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	11	0.27499999999999997	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	11	0.27499999999999997	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	11	0.27499999999999997	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	11	0.27499999999999997	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	11	0.27499999999999997	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	11	0.27499999999999997	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	10	0.25	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	10	0.25	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	10	0.25	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	10	0.25	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	10	0.25	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	10	0.25	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	10	0.25	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	10	0.25	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	10	0.25	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	10	0.25	No Hit
GTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAAC	10	0.25	No Hit
CCCAGCTCAAACATTACCTCAATGGTGATGAGGATGACGATCATCCACTC	9	0.22499999999999998	No Hit
AGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATC	9	0.22499999999999998	No Hit
CTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGT	9	0.22499999999999998	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	9	0.22499999999999998	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	9	0.22499999999999998	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	9	0.22499999999999998	No Hit
CTCGCTTCTCATTGAGGTGATTCCGCATTAGGTCTGTTAGTTCCATGCAG	9	0.22499999999999998	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	9	0.22499999999999998	No Hit
GTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACC	9	0.22499999999999998	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	9	0.22499999999999998	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	9	0.22499999999999998	No Hit
TTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAA	9	0.22499999999999998	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	9	0.22499999999999998	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	9	0.22499999999999998	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	9	0.22499999999999998	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	9	0.22499999999999998	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	9	0.22499999999999998	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	8	0.2	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	8	0.2	No Hit
GGCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTA	8	0.2	No Hit
ATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTC	8	0.2	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	8	0.2	No Hit
CCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCT	8	0.2	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	8	0.2	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	8	0.2	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	8	0.2	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	8	0.2	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	8	0.2	No Hit
TTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTA	8	0.2	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	8	0.2	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	8	0.2	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	8	0.2	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	8	0.2	No Hit
GGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAA	8	0.2	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	8	0.2	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	8	0.2	No Hit
CTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAG	7	0.17500000000000002	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	7	0.17500000000000002	No Hit
TTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACATT	7	0.17500000000000002	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	7	0.17500000000000002	No Hit
GGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCCTT	7	0.17500000000000002	No Hit
CTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTA	7	0.17500000000000002	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	7	0.17500000000000002	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	7	0.17500000000000002	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	7	0.17500000000000002	No Hit
CCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTA	7	0.17500000000000002	No Hit
AGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCT	7	0.17500000000000002	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	7	0.17500000000000002	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	7	0.17500000000000002	No Hit
GTCCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCC	7	0.17500000000000002	No Hit
CTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGG	7	0.17500000000000002	No Hit
CTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTT	7	0.17500000000000002	No Hit
ATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCGTATTT	7	0.17500000000000002	No Hit
ATTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAA	7	0.17500000000000002	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	7	0.17500000000000002	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	6	0.15	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	6	0.15	No Hit
CACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTAT	6	0.15	No Hit
CTCCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGT	6	0.15	No Hit
GTCACAATCAGGACCATGAACCACTGAGAGAAACCAGAGATGAAACCAGA	6	0.15	No Hit
TTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGAACATGT	6	0.15	No Hit
AGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACA	6	0.15	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	6	0.15	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	6	0.15	No Hit
GGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATT	6	0.15	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	6	0.15	No Hit
GCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTAG	6	0.15	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	6	0.15	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	6	0.15	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	6	0.15	No Hit
ATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGC	6	0.15	No Hit
CCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGC	6	0.15	No Hit
TATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACT	6	0.15	No Hit
TCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCT	6	0.15	No Hit
GTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGC	6	0.15	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	6	0.15	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	6	0.15	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	6	0.15	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	6	0.15	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	6	0.15	No Hit
GTTGCACTTGCCCGGCGGCGGCCACCGTAGTCACCGGCGCCGAGTCCTCC	6	0.15	No Hit
CTCTGGTTGAGGCACACGTGGCGTGCGGCCTGGTTGGCCCTACAGAAGCT	6	0.15	No Hit
AAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACAC	6	0.15	No Hit
CCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTG	6	0.15	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	6	0.15	No Hit
CTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCTCACAT	6	0.15	No Hit
TGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTT	6	0.15	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	6	0.15	No Hit
CAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGAT	5	0.125	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	5	0.125	No Hit
ATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGTGGA	5	0.125	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	5	0.125	No Hit
CTCATCTGTGAGAGCGGCCGCTGCACTCTTCGCACCCCTCATACACTACC	5	0.125	No Hit
CGATCATCCACTCCAAGCGGAGTGCTCGCTTCTCATTGAGGTGATTCCGC	5	0.125	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	5	0.125	No Hit
TAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTC	5	0.125	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	5	0.125	No Hit
GCAGAGATGAGTGCGTTTCCCTGTGTAGCCAGGGCTGCTCTGGACATCAG	5	0.125	No Hit
CGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTAC	5	0.125	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	5	0.125	No Hit
CCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATC	5	0.125	No Hit
AAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACA	5	0.125	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	5	0.125	No Hit
TAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACA	5	0.125	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	5	0.125	No Hit
GGGCAGTCTTGAAAAGAGGAAATGTTAGCTGGCTCTAGCTCCCGATGCAG	5	0.125	No Hit
CAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACACACACACA	5	0.125	No Hit
CTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGAACAT	5	0.125	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	5	0.125	No Hit
GAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCG	5	0.125	No Hit
GGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTT	5	0.125	No Hit
TGAGTGATCCATGAGACATGCACATAATACAGTATGTTTGTAGGGAGACC	5	0.125	No Hit
CTCCAAGTTGTCGATGTCTGCAATGTCTGAGGGCGGTGGGGTCTCCGACT	5	0.125	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	5	0.125	No Hit
AGGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAAT	5	0.125	No Hit
GTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTG	5	0.125	No Hit
GTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGC	5	0.125	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAAC	5	0.125	No Hit
TAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAAC	5	0.125	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	5	0.125	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	5	0.125	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	5	0.125	No Hit
GCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGA	5	0.125	No Hit
AGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	5	0.125	No Hit
ACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAG	5	0.125	No Hit
TCCTGTTCTTCCGTATCTAGCATGCCACCCTCTACCTCCTGAACTGGGGT	5	0.125	No Hit
ATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGG	5	0.125	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	5	0.125	No Hit
GTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0375	0.0	0.0	0.0	0.0
70-71	0.225	0.0	0.0	0.0	0.0
72-73	0.3625	0.0	0.0	0.0	0.0
74-75	0.4375	0.0	0.0	0.0	0.0
76-77	0.5875	0.0	0.0	0.0	0.0
78-79	0.6625	0.0	0.0	0.0	0.0
80-81	0.875	0.0	0.0	0.0	0.0
82-83	0.9625	0.0	0.0	0.0	0.0
84-85	1.0375	0.0	0.0	0.0	0.0
86-87	1.1125	0.0	0.0	0.0	0.0
88-89	1.1625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882421 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882421_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.257	37.0	37.0	37.0	37.0	37.0
2	36.2645	37.0	37.0	37.0	37.0	37.0
3	36.3865	37.0	37.0	37.0	37.0	37.0
4	36.4435	37.0	37.0	37.0	37.0	37.0
5	36.393	37.0	37.0	37.0	37.0	37.0
6	36.343	37.0	37.0	37.0	37.0	37.0
7	36.38325	37.0	37.0	37.0	37.0	37.0
8	36.37	37.0	37.0	37.0	37.0	37.0
9	36.488	37.0	37.0	37.0	37.0	37.0
10-11	36.43025	37.0	37.0	37.0	37.0	37.0
12-13	36.42975	37.0	37.0	37.0	37.0	37.0
14-15	36.4585	37.0	37.0	37.0	37.0	37.0
16-17	36.50775	37.0	37.0	37.0	37.0	37.0
18-19	36.40225	37.0	37.0	37.0	37.0	37.0
20-21	36.36975	37.0	37.0	37.0	37.0	37.0
22-23	36.35575	37.0	37.0	37.0	37.0	37.0
24-25	36.305	37.0	37.0	37.0	37.0	37.0
26-27	36.3435	37.0	37.0	37.0	37.0	37.0
28-29	36.2745	37.0	37.0	37.0	37.0	37.0
30-31	36.3405	37.0	37.0	37.0	37.0	37.0
32-33	36.30675	37.0	37.0	37.0	37.0	37.0
34-35	36.2395	37.0	37.0	37.0	37.0	37.0
36-37	36.32875	37.0	37.0	37.0	37.0	37.0
38-39	36.231	37.0	37.0	37.0	37.0	37.0
40-41	36.247749999999996	37.0	37.0	37.0	37.0	37.0
42-43	36.2915	37.0	37.0	37.0	37.0	37.0
44-45	36.232	37.0	37.0	37.0	37.0	37.0
46-47	36.126000000000005	37.0	37.0	37.0	37.0	37.0
48-49	36.097	37.0	37.0	37.0	37.0	37.0
50-51	36.18275	37.0	37.0	37.0	37.0	37.0
52-53	36.093500000000006	37.0	37.0	37.0	37.0	37.0
54-55	36.0465	37.0	37.0	37.0	37.0	37.0
56-57	36.04125	37.0	37.0	37.0	37.0	37.0
58-59	36.0115	37.0	37.0	37.0	37.0	37.0
60-61	36.0615	37.0	37.0	37.0	37.0	37.0
62-63	35.98075	37.0	37.0	37.0	37.0	37.0
64-65	36.001999999999995	37.0	37.0	37.0	37.0	37.0
66-67	35.933	37.0	37.0	37.0	37.0	37.0
68-69	35.9545	37.0	37.0	37.0	37.0	37.0
70-71	35.93	37.0	37.0	37.0	37.0	37.0
72-73	35.88475	37.0	37.0	37.0	37.0	37.0
74-75	35.920500000000004	37.0	37.0	37.0	37.0	37.0
76-77	35.9015	37.0	37.0	37.0	37.0	37.0
78-79	35.924499999999995	37.0	37.0	37.0	37.0	37.0
80-81	35.82925	37.0	37.0	37.0	37.0	37.0
82-83	35.7655	37.0	37.0	37.0	37.0	37.0
84-85	35.6935	37.0	37.0	37.0	37.0	37.0
86-87	35.70325	37.0	37.0	37.0	37.0	37.0
88-89	35.765	37.0	37.0	37.0	37.0	37.0
90-91	35.74975	37.0	37.0	37.0	37.0	37.0
92-93	35.641125	37.0	37.0	37.0	37.0	37.0
94-95	35.673249999999996	37.0	37.0	37.0	37.0	37.0
96-97	35.732	37.0	37.0	37.0	37.0	37.0
98-99	35.61425	37.0	37.0	37.0	37.0	37.0
100-101	35.205	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	1.0
21	0.0
22	1.0
23	2.0
24	2.0
25	2.0
26	7.0
27	11.0
28	11.0
29	13.0
30	24.0
31	30.0
32	39.0
33	91.0
34	172.0
35	542.0
36	2709.0
37	342.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.78557114228457	18.662324649298597	13.10120240480962	25.450901803607213
2	19.25	26.5	36.35	17.9
3	20.1	27.3	32.15	20.45
4	25.275	33.45	22.400000000000002	18.875
5	25.324999999999996	32.324999999999996	23.849999999999998	18.5
6	22.175	34.625	23.599999999999998	19.6
7	20.880220055013755	22.305576394098527	37.38434608652163	19.42985746436609
8	25.174999999999997	21.4	29.349999999999998	24.075
9	24.3	25.025	27.325	23.35
10-11	24.337500000000002	31.9625	22.375	21.325
12-13	25.775	25.575	26.5875	22.0625
14-15	24.6875	26.4125	29.4125	19.4875
16-17	24.2375	28.787499999999998	27.825	19.15
18-19	22.912499999999998	29.599999999999998	27.3375	20.150000000000002
20-21	21.95	30.2875	28.425	19.3375
22-23	23.2375	28.199999999999996	26.637499999999996	21.925
24-25	23.175	27.775	26.5375	22.5125
26-27	23.1875	27.3875	26.775	22.650000000000002
28-29	23.75	27.9125	27.275	21.0625
30-31	23.5375	27.400000000000002	29.15	19.9125
32-33	23.7375	27.737499999999997	28.95	19.575
34-35	23.8625	29.475	26.2875	20.375
36-37	22.85	27.325	29.1375	20.6875
38-39	22.3875	27.1375	28.475	22.0
40-41	21.8	27.950000000000003	28.925	21.325
42-43	22.3375	29.2	28.575	19.8875
44-45	22.7125	27.925	29.875	19.4875
46-47	23.5625	28.299999999999997	24.962500000000002	23.175
48-49	23.5625	26.5625	30.575000000000003	19.3
50-51	21.2625	28.6875	27.537499999999998	22.5125
52-53	23.5625	28.8625	28.4125	19.162499999999998
54-55	24.45	28.9375	26.5875	20.025000000000002
56-57	22.675	29.2	27.762500000000003	20.3625
58-59	21.50537634408602	29.669917479369843	26.481620405101275	22.343085771442862
60-61	21.25	29.9875	28.275	20.4875
62-63	21.8	28.787499999999998	26.8375	22.575
64-65	24.4125	27.8625	28.8375	18.8875
66-67	22.7125	26.2875	28.462500000000002	22.537499999999998
68-69	24.3875	27.737499999999997	28.1625	19.7125
70-71	23.0625	30.4375	27.500000000000004	19.0
72-73	22.900000000000002	29.025000000000002	26.3625	21.712500000000002
74-75	23.474999999999998	27.1	27.212500000000002	22.2125
76-77	21.512500000000003	29.349999999999998	26.337500000000002	22.8
78-79	23.5625	26.9625	28.6625	20.8125
80-81	23.025000000000002	27.462500000000002	29.625	19.8875
82-83	22.575	28.0875	28.499999999999996	20.837500000000002
84-85	22.125	25.7625	29.0875	23.025000000000002
86-87	22.7	30.112499999999997	26.25	20.9375
88-89	24.275	27.9125	26.700000000000003	21.1125
90-91	25.0125	28.537499999999998	25.7125	20.7375
92-93	23.27790973871734	30.25378172271534	26.440805100637583	20.02750343792974
94-95	22.3625	30.4625	26.7125	20.4625
96-97	24.099999999999998	27.8625	26.625	21.4125
98-99	22.0	29.362500000000004	27.900000000000002	20.7375
100-101	22.7375	27.950000000000003	27.437499999999996	21.875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.5
23	3.0
24	2.0
25	1.5
26	1.0
27	1.5
28	5.0
29	9.5
30	48.5
31	125.5
32	182.0
33	159.5
34	146.0
35	133.5
36	104.5
37	105.5
38	93.5
39	88.5
40	107.0
41	133.0
42	173.5
43	189.0
44	207.0
45	218.0
46	180.0
47	192.5
48	196.0
49	157.5
50	127.0
51	108.5
52	86.5
53	64.0
54	61.0
55	104.5
56	97.0
57	48.0
58	58.0
59	54.5
60	32.5
61	29.0
62	30.0
63	15.0
64	3.5
65	1.5
66	1.0
67	1.0
68	0.5
69	7.0
70	9.0
71	8.5
72	10.5
73	9.5
74	8.5
75	6.0
76	11.5
77	18.5
78	14.5
79	6.5
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.025
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.79113539769278	21.325
2	20.704310868245294	17.05
3	9.289617486338798	11.475
4	6.6788099574984825	11.0
5	3.1572556162720096	6.5
6	2.0643594414086217	5.1
7	1.3964784456587735	4.025
8	1.214329083181542	4.0
9	0.7285974499089253	2.7
>10	2.9751062537947783	16.825000000000003
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	30	0.75	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	29	0.7250000000000001	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	27	0.675	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	22	0.5499999999999999	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	21	0.525	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	20	0.5	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	20	0.5	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	19	0.475	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	17	0.42500000000000004	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	16	0.4	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	16	0.4	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	16	0.4	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	15	0.375	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	14	0.35000000000000003	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	14	0.35000000000000003	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	14	0.35000000000000003	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	14	0.35000000000000003	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	13	0.325	No Hit
GTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTT	13	0.325	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	12	0.3	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	12	0.3	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	12	0.3	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	12	0.3	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	12	0.3	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	12	0.3	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	11	0.27499999999999997	No Hit
GGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGG	11	0.27499999999999997	No Hit
GTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTG	11	0.27499999999999997	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	11	0.27499999999999997	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	11	0.27499999999999997	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	11	0.27499999999999997	No Hit
TTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACAC	11	0.27499999999999997	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	11	0.27499999999999997	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	11	0.27499999999999997	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	11	0.27499999999999997	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	11	0.27499999999999997	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	10	0.25	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	10	0.25	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	10	0.25	No Hit
GTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTT	10	0.25	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	10	0.25	No Hit
GGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGA	10	0.25	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	10	0.25	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	10	0.25	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	10	0.25	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	10	0.25	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	10	0.25	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	10	0.25	No Hit
GCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATA	10	0.25	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	9	0.22499999999999998	No Hit
GCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCC	9	0.22499999999999998	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	9	0.22499999999999998	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	9	0.22499999999999998	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	9	0.22499999999999998	No Hit
CTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGT	9	0.22499999999999998	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	9	0.22499999999999998	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	9	0.22499999999999998	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	9	0.22499999999999998	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	9	0.22499999999999998	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	9	0.22499999999999998	No Hit
GTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATG	9	0.22499999999999998	No Hit
GTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTT	8	0.2	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	8	0.2	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	8	0.2	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	8	0.2	No Hit
GCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAA	8	0.2	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	8	0.2	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	8	0.2	No Hit
CTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGT	8	0.2	No Hit
CAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCC	8	0.2	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	8	0.2	No Hit
GGCGGCCGGTGGCGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTC	8	0.2	No Hit
GATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCT	8	0.2	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	8	0.2	No Hit
ATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTG	8	0.2	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	8	0.2	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	8	0.2	No Hit
ACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTT	8	0.2	No Hit
GCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTAC	8	0.2	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	8	0.2	No Hit
GAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTG	8	0.2	No Hit
GTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACA	7	0.17500000000000002	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	7	0.17500000000000002	No Hit
TCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAAT	7	0.17500000000000002	No Hit
ATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCT	7	0.17500000000000002	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	7	0.17500000000000002	No Hit
ATGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACA	7	0.17500000000000002	No Hit
GTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGG	7	0.17500000000000002	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	7	0.17500000000000002	No Hit
CCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTAC	7	0.17500000000000002	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	7	0.17500000000000002	No Hit
TTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTA	7	0.17500000000000002	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	7	0.17500000000000002	No Hit
GTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCT	7	0.17500000000000002	No Hit
CTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTCTC	7	0.17500000000000002	No Hit
CCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGC	7	0.17500000000000002	No Hit
GAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGC	7	0.17500000000000002	No Hit
GTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTC	7	0.17500000000000002	No Hit
CAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAA	7	0.17500000000000002	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	7	0.17500000000000002	No Hit
CCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAG	7	0.17500000000000002	No Hit
GAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAA	7	0.17500000000000002	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	7	0.17500000000000002	No Hit
TCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTT	7	0.17500000000000002	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	6	0.15	No Hit
CTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTG	6	0.15	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	6	0.15	No Hit
TGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTG	6	0.15	No Hit
ACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTT	6	0.15	No Hit
ATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACA	6	0.15	No Hit
AGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCT	6	0.15	No Hit
CCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTA	6	0.15	No Hit
GTGCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTGAAATA	6	0.15	No Hit
GGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACAC	6	0.15	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	6	0.15	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	6	0.15	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	6	0.15	No Hit
AGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACA	6	0.15	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	6	0.15	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	6	0.15	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	6	0.15	No Hit
GCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCA	6	0.15	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	6	0.15	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	6	0.15	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	6	0.15	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	6	0.15	No Hit
TTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTG	6	0.15	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	6	0.15	No Hit
GGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTG	6	0.15	No Hit
AATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAAT	6	0.15	No Hit
GTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAA	6	0.15	No Hit
TTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTGAACACA	6	0.15	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	6	0.15	No Hit
GTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTT	6	0.15	No Hit
TATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCT	6	0.15	No Hit
TCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTA	6	0.15	No Hit
GAGGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCAC	6	0.15	No Hit
GAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTG	6	0.15	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	5	0.125	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	5	0.125	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	5	0.125	No Hit
ATTTCATTGGTTGAGATGCTTTGCCAGGTCATGTACTTATTGCCTCATTG	5	0.125	No Hit
GTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAA	5	0.125	No Hit
AACCAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATG	5	0.125	No Hit
AGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTC	5	0.125	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	5	0.125	No Hit
CATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAA	5	0.125	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	5	0.125	No Hit
GAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAA	5	0.125	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	5	0.125	No Hit
CAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAG	5	0.125	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	5	0.125	No Hit
CGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGG	5	0.125	No Hit
CCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTT	5	0.125	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	5	0.125	No Hit
GGAAGATACAAGTCTTTTTAAAGTTTACTGATGCTTAACTCTGTGGGCTC	5	0.125	No Hit
GTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGAT	5	0.125	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	5	0.125	No Hit
TCCACACCGGGGTACCCTTCACTGCGGGCCCGCCAGGGGAAGGCCGTGGG	5	0.125	No Hit
ATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCC	5	0.125	No Hit
CCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCA	5	0.125	No Hit
CGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTCCCCGCGGTGGAA	5	0.125	No Hit
TTATGACAAGACCTGCCAGTTCCTTAGCATCACGCGAAGAGTTAAGGTCA	5	0.125	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	5	0.125	No Hit
GACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGA	5	0.125	No Hit
CTTTATGACAAGACCTGCCAGTTCCTTAGCATCACGCGAAGAGTTAAGGT	5	0.125	No Hit
CTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAAT	5	0.125	No Hit
CCTTCCAGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGA	5	0.125	No Hit
ACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAA	5	0.125	No Hit
ACCAGATCCTAGTACGGAATGCACACCCACCAGGGAGCACTCTCTGTCAT	5	0.125	No Hit
CTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTT	5	0.125	No Hit
AGAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGA	5	0.125	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	5	0.125	No Hit
GTCCATTCCAGAGGCATTAAAAGCTGGGAAGAAAGTCAAACTGTCTCATA	5	0.125	No Hit
GACGCCACCAGCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTT	5	0.125	No Hit
GAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAA	5	0.125	No Hit
CCAATGTCGGCAATACCTAGTCCCACTTCTCAAACTGGAGCCTCCTCCTT	5	0.125	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	5	0.125	No Hit
TCAAAGTTCTAAAGGAAAAAGAATGACTAGGCCAACTTTCAACACCATTC	5	0.125	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	5	0.125	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	5	0.125	No Hit
AAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAA	5	0.125	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	5	0.125	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	5	0.125	No Hit
GTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATAC	5	0.125	No Hit
CCTTCTCCGGGCCACTCTCAGGCTCTGGCGCGCGGGGACATGTCGGTGGT	5	0.125	No Hit
CTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAA	5	0.125	No Hit
AAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAA	5	0.125	No Hit
CCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCT	5	0.125	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0375	0.0	0.0	0.0	0.0
70-71	0.225	0.0	0.0	0.0	0.0
72-73	0.35	0.0	0.0	0.0	0.0
74-75	0.4125	0.0	0.0	0.0	0.0
76-77	0.5625	0.0	0.0	0.0	0.0
78-79	0.6375	0.0	0.0	0.0	0.0
80-81	0.85	0.0	0.0	0.0	0.0
82-83	0.9375	0.0	0.0	0.0	0.0
84-85	1.0125	0.0	0.0	0.0	0.0
86-87	1.0875	0.0	0.0	0.0	0.0
88-89	1.1625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916461 spots for SRR24882421.sra
Written 3916461 spots for SRR24882421.sra
Read 3916466 spots for SRR24882421.sra
Written 3916466 spots for SRR24882421.sra
SRR ids: ['SRR24882421.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qq0i97af
SRR24882421.sra spots: 31331693
blocks: [[1, 3916461], [3916462, 7832922], [7832923, 11749383], [11749384, 15665844], [15665845, 19582305], [19582306, 23498766], [23498767, 27415227], [27415228, 31331693]]
SRR24882421 file size 7566448
SRR24882421 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882421 SRR24882421_1.fastq SRR24882421_2.fastq
Input file:	SRR24882421_1.fastq
Paired file:	SRR24882421_2.fastq
trimmed:	SRR24882421-trimmed-pair1.fastq, SRR24882421-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 03:54:38 2026 >> started

Fri Jul  3 03:55:06 2026 >> done (28.320s)
31331693 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
31331693 (100.00%) read pairs available; of these:
  958391 ( 3.06%) trimmed read pairs available after processing
30373302 (96.94%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 61	       1	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       6	  0.00%
 66	      30	  0.00%
 67	    2087	  0.01%
 68	   10484	  0.03%
 69	   12291	  0.04%
 70	   13690	  0.04%
 71	   14367	  0.05%
 72	   15991	  0.05%
 73	   16709	  0.05%
 74	   17477	  0.06%
 75	   18959	  0.06%
 76	   19712	  0.06%
 77	   20795	  0.07%
 78	   21289	  0.07%
 79	   22419	  0.07%
 80	   23167	  0.07%
 81	   24045	  0.08%
 82	   24919	  0.08%
 83	   26299	  0.08%
 84	   27434	  0.09%
 85	   28345	  0.09%
 86	   28629	  0.09%
 87	   29677	  0.09%
 88	   31450	  0.10%
 89	   31443	  0.10%
 90	   34143	  0.11%
 91	   35145	  0.11%
 92	   35461	  0.11%
 93	   36438	  0.12%
 94	   38026	  0.12%
 95	   38733	  0.12%
 96	   39592	  0.13%
 97	   42190	  0.13%
 98	   42933	  0.14%
 99	   45568	  0.15%
100	   88447	  0.28%
101	30373302	 96.94%
31331693 reads passed initial QC


criterion=sequence-density
sequence-density=0.50
sequence-density-rank=1
fanout-score=14.62
fanout-score-rank=7
prefix-density=2.50
prefix-fanout=2.9
sequence=TGGAGAAGATGTGGGGTGGGGCCTCCTGGCGCTTTTTGCCTCGGTAGGCTGCCACCACCTCAGGGTTGTACACCGGCAGCCACTTGTAGGGGTTGACGGTGACACAAAAGAGGCCTGAGTAAGTGTAGATCATCCAGGCTGCATAACGCTCTTTGAGGTTGTACAGCACAGCGGGCTCGTGCAGGTGGGTCATCATGGCCATGTCCTCAATCTTGTCGTACTTGGGAGGGTTCATGGAGAAGACTTGATCATCCTTGACGGTGACCGTAGCTCCGCCTTCGGTCTTGGCTGTCACCTTCCCCCCTTCCCTGCTCTGCACTGTTGCTTTCACATAGGACTCCTTAGCATCCACCACAAACACCGATGACTTGGCATCAAAAGGCTTGTTCTGGGCCTCGATTCGCTCCTTTTCAGACTTCCGGAGGTAAGGAGCAGCCTCCCCGAAAACGGCCATCTCAGCGTCGGAACTCATGGCTGCGGGCTATTGGTGGCAGCTCAGGGCCAGGTGCAC


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=29
fanout-score=36.43
fanout-score-rank=1
prefix-density=0.42
prefix-fanout=4.2
sequence=CCAGCTTCTTCCAGCCTCTCGCTGATCTCCTCCAGTTCCCGGGAGAGGTCAGAGCGCTGCTTCTCTGCTTTGGCCCTGGAGGCCCGCTCTGCCTCGATTTCCTCCTCCAGCTCCTCGATGCGGGCCTGCAACTCCTTGATCTTCTTCTGCAGCTGCATGCCGAGGGCCTGCTCGTCCTCAATCT


criterion=sequence-density
sequence-density=1.64
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=36
prefix-density=1.61
prefix-fanout=2.0
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=28
fanout-score=42.71
fanout-score-rank=1
prefix-density=0.52
prefix-fanout=5.2
sequence=GGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGATGAGGAGATC
SRR24882421 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 03:55:18
                             Started mapping on |	Jul 03 03:55:18
                                    Finished on |	Jul 03 04:16:25
       Mapping speed, Million of reads per hour |	89.02

                          Number of input reads |	31331693
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27580599
                        Uniquely mapped reads % |	88.03%
                          Average mapped length |	200.37
                       Number of splices: Total |	20182340
            Number of splices: Annotated (sjdb) |	20023332
                       Number of splices: GT/AG |	20029622
                       Number of splices: GC/AG |	121340
                       Number of splices: AT/AC |	12346
               Number of splices: Non-canonical |	19032
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	3.01
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.63
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3739613
             % of reads mapped to multiple loci |	11.94%
        Number of reads mapped to too many loci |	4418
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.02%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	11481	11481	11481
N_multimapping	3739613	3739613	3739613
N_noFeature	1574238	24900391	2197751
N_ambiguous	2316784	702993	245150
UnstrandedReadsAssigned:23689577 PositiveStrandReadsAssigned:1977215 NegativeStrandReadsAssigned:25137698
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882421 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882421-trimmed-pair1.fastq
                             SRR24882421-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,331,693 reads, 27,019,413 reads pseudoaligned
[quant] estimated average fragment length: 230.404
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,296 rounds

 109283 SRR24882421.ke.tsv
  52636 SRR24882421.se.tsv
 161919 total
==> SRR24882421.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8	0	0
ENSMUST00000196221.1	9	1	0	0
ENSMUST00000179664.1	11	3	0	0
ENSMUST00000178537.1	12	4	0	0
ENSMUST00000178862.1	14	6	0	0
ENSMUST00000179520.1	11	3	0	0
ENSMUST00000179883.1	16	8	0	0
ENSMUST00000195858.1	10	2	0	0
ENSMUST00000179932.1	12	4	0	0

==> SRR24882421.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882421 completed mapping pipeline successfully
