Starting /dee2/code/volunteer_pipeline.sh SRR24882419
    current disk space = 438586212352
    free memory = 337232204 
SRR24882419 SRAfilesize
3b82e4d7cde447dec9ac71faabf5e846  SRR24882419.sra
SRR24882419.sra file validated
SRR24882419 is paired end
SRR24882419 is conventional basespace
SRR24882419 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.643	37.0	37.0	37.0	37.0	37.0
2	36.4985	37.0	37.0	37.0	37.0	37.0
3	36.601	37.0	37.0	37.0	37.0	37.0
4	36.6405	37.0	37.0	37.0	37.0	37.0
5	36.65	37.0	37.0	37.0	37.0	37.0
6	36.5805	37.0	37.0	37.0	37.0	37.0
7	36.5965	37.0	37.0	37.0	37.0	37.0
8	36.5505	37.0	37.0	37.0	37.0	37.0
9	36.653	37.0	37.0	37.0	37.0	37.0
10-11	36.5905	37.0	37.0	37.0	37.0	37.0
12-13	36.634249999999994	37.0	37.0	37.0	37.0	37.0
14-15	36.583	37.0	37.0	37.0	37.0	37.0
16-17	36.63175	37.0	37.0	37.0	37.0	37.0
18-19	36.575500000000005	37.0	37.0	37.0	37.0	37.0
20-21	36.53275	37.0	37.0	37.0	37.0	37.0
22-23	36.5745	37.0	37.0	37.0	37.0	37.0
24-25	36.55075	37.0	37.0	37.0	37.0	37.0
26-27	36.583	37.0	37.0	37.0	37.0	37.0
28-29	36.52825	37.0	37.0	37.0	37.0	37.0
30-31	36.54625	37.0	37.0	37.0	37.0	37.0
32-33	36.445	37.0	37.0	37.0	37.0	37.0
34-35	36.50025	37.0	37.0	37.0	37.0	37.0
36-37	36.476	37.0	37.0	37.0	37.0	37.0
38-39	36.441500000000005	37.0	37.0	37.0	37.0	37.0
40-41	36.4815	37.0	37.0	37.0	37.0	37.0
42-43	36.4825	37.0	37.0	37.0	37.0	37.0
44-45	36.476	37.0	37.0	37.0	37.0	37.0
46-47	36.50125	37.0	37.0	37.0	37.0	37.0
48-49	36.40375	37.0	37.0	37.0	37.0	37.0
50-51	36.4175	37.0	37.0	37.0	37.0	37.0
52-53	36.419250000000005	37.0	37.0	37.0	37.0	37.0
54-55	36.386250000000004	37.0	37.0	37.0	37.0	37.0
56-57	36.42125	37.0	37.0	37.0	37.0	37.0
58-59	36.41725	37.0	37.0	37.0	37.0	37.0
60-61	36.436499999999995	37.0	37.0	37.0	37.0	37.0
62-63	36.408500000000004	37.0	37.0	37.0	37.0	37.0
64-65	36.39225	37.0	37.0	37.0	37.0	37.0
66-67	36.34725	37.0	37.0	37.0	37.0	37.0
68-69	36.343	37.0	37.0	37.0	37.0	37.0
70-71	36.282	37.0	37.0	37.0	37.0	37.0
72-73	36.364000000000004	37.0	37.0	37.0	37.0	37.0
74-75	36.3705	37.0	37.0	37.0	37.0	37.0
76-77	36.28875	37.0	37.0	37.0	37.0	37.0
78-79	36.2525	37.0	37.0	37.0	37.0	37.0
80-81	36.324749999999995	37.0	37.0	37.0	37.0	37.0
82-83	36.27575	37.0	37.0	37.0	37.0	37.0
84-85	36.2085	37.0	37.0	37.0	37.0	37.0
86-87	36.24675	37.0	37.0	37.0	37.0	37.0
88-89	36.232	37.0	37.0	37.0	37.0	37.0
90-91	36.264250000000004	37.0	37.0	37.0	37.0	37.0
92-93	36.143	37.0	37.0	37.0	37.0	37.0
94-95	36.201	37.0	37.0	37.0	37.0	37.0
96-97	36.071749999999994	37.0	37.0	37.0	37.0	37.0
98-99	36.1205	37.0	37.0	37.0	37.0	37.0
100-101	36.051500000000004	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	2.0
27	4.0
28	10.0
29	21.0
30	7.0
31	35.0
32	30.0
33	55.0
34	76.0
35	244.0
36	2706.0
37	808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.125	10.424999999999999	14.524999999999999	36.925000000000004
2	18.375	14.725	37.824999999999996	29.075
3	20.045045045045047	19.26926926926927	31.756756756756754	28.928928928928926
4	24.325	29.549999999999997	22.7	23.425
5	23.5	31.674999999999997	24.425	20.4
6	21.125	34.625	27.400000000000002	16.85
7	15.950000000000001	25.324999999999996	43.25	15.475
8	20.45	22.45	32.5	24.6
9	19.575	22.425	35.025	22.975
10-11	20.075000000000003	32.5125	25.874999999999996	21.5375
12-13	21.85	25.575	28.749999999999996	23.825
14-15	20.8	27.250000000000004	29.125	22.825
16-17	20.5375	28.4375	27.3875	23.6375
18-19	23.1	28.999999999999996	26.2625	21.637500000000003
20-21	21.55	28.512500000000003	27.787499999999998	22.15
22-23	22.7	27.487499999999997	26.5375	23.275000000000002
24-25	23.4125	28.1	26.3125	22.175
26-27	21.675	28.775000000000002	27.8375	21.712500000000002
28-29	20.7625	28.1125	28.237499999999997	22.8875
30-31	21.875	26.150000000000002	28.849999999999998	23.125
32-33	22.475	26.900000000000002	28.8875	21.7375
34-35	19.7625	27.5125	29.049999999999997	23.674999999999997
36-37	22.537499999999998	27.212500000000002	29.2	21.05
38-39	20.5	28.7375	29.4375	21.325
40-41	20.837500000000002	28.275	29.45	21.4375
42-43	21.775	30.412499999999998	26.674999999999997	21.1375
44-45	20.075000000000003	29.5375	26.437500000000004	23.95
46-47	22.675	29.95	24.775	22.6
48-49	21.325	30.912499999999998	25.900000000000002	21.8625
50-51	23.2125	26.637499999999996	26.525	23.625
52-53	21.2875	26.1	29.6375	22.975
54-55	21.3	26.174999999999997	28.712500000000002	23.8125
56-57	19.950000000000003	27.3875	29.525000000000002	23.1375
58-59	21.1125	27.125	28.1125	23.65
60-61	21.125	28.925	26.75	23.200000000000003
62-63	23.9875	27.462500000000002	27.487499999999997	21.0625
64-65	22.175	26.0125	27.925	23.8875
66-67	22.650000000000002	27.275	27.9125	22.162499999999998
68-69	21.4375	27.325	27.700000000000003	23.5375
70-71	23.4875	27.625	27.1125	21.775
72-73	21.099999999999998	28.999999999999996	27.675	22.225
74-75	21.8	26.8375	28.199999999999996	23.1625
76-77	23.7375	27.9125	26.137500000000003	22.2125
78-79	24.587500000000002	25.75	28.65	21.0125
80-81	23.8625	26.187500000000004	28.075	21.875
82-83	22.5625	28.15	27.675	21.6125
84-85	22.475	28.000000000000004	26.125	23.400000000000002
86-87	21.712500000000002	27.6875	27.6625	22.9375
88-89	23.575	26.787499999999998	29.062500000000004	20.575
90-91	23.1375	26.75	27.250000000000004	22.8625
92-93	21.7	27.200000000000003	28.512500000000003	22.5875
94-95	22.2	29.049999999999997	26.474999999999998	22.275
96-97	21.65	27.6625	25.4875	25.2
98-99	21.3875	28.000000000000004	28.3625	22.25
100-101	22.4625	27.6125	28.1	21.825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	0.0
21	0.5
22	1.5
23	2.5
24	3.5
25	15.5
26	23.0
27	12.5
28	8.5
29	20.5
30	39.0
31	62.0
32	107.0
33	126.0
34	122.0
35	136.5
36	127.0
37	108.5
38	83.5
39	67.0
40	96.0
41	152.5
42	206.5
43	191.5
44	150.0
45	148.5
46	207.5
47	243.5
48	176.0
49	126.0
50	144.0
51	143.5
52	110.5
53	88.5
54	90.0
55	95.5
56	85.0
57	69.5
58	76.5
59	100.5
60	85.0
61	45.0
62	29.0
63	20.5
64	7.5
65	2.0
66	2.5
67	7.5
68	7.0
69	4.0
70	3.5
71	2.0
72	0.5
73	1.5
74	3.0
75	2.5
76	1.0
77	1.0
78	1.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	1.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.1
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.90570719602977	33.2
2	17.07196029776675	17.2
3	6.302729528535981	9.525
4	3.1761786600496276	6.4
5	1.6377171215880892	4.125
6	1.0421836228287842	3.15
7	1.240694789081886	4.375
8	1.0918114143920596	4.3999999999999995
9	0.19851116625310172	0.8999999999999999
>10	2.3325062034739457	16.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	38	0.95	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	27	0.675	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	24	0.6	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	22	0.5499999999999999	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	19	0.475	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	19	0.475	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	18	0.44999999999999996	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	18	0.44999999999999996	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	18	0.44999999999999996	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	17	0.42500000000000004	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	17	0.42500000000000004	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	17	0.42500000000000004	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	17	0.42500000000000004	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	17	0.42500000000000004	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	14	0.35000000000000003	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	14	0.35000000000000003	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	14	0.35000000000000003	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	14	0.35000000000000003	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	13	0.325	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	13	0.325	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	13	0.325	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	13	0.325	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	12	0.3	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	12	0.3	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	12	0.3	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	12	0.3	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	12	0.3	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	11	0.27499999999999997	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	11	0.27499999999999997	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	11	0.27499999999999997	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	11	0.27499999999999997	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	11	0.27499999999999997	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	11	0.27499999999999997	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	11	0.27499999999999997	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	11	0.27499999999999997	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	11	0.27499999999999997	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	11	0.27499999999999997	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	11	0.27499999999999997	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	11	0.27499999999999997	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	11	0.27499999999999997	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	10	0.25	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	10	0.25	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	10	0.25	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	10	0.25	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	10	0.25	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	10	0.25	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	10	0.25	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	9	0.22499999999999998	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	9	0.22499999999999998	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	9	0.22499999999999998	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	9	0.22499999999999998	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	8	0.2	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	8	0.2	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	8	0.2	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	8	0.2	No Hit
GCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAG	8	0.2	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	8	0.2	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	8	0.2	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	8	0.2	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	8	0.2	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	8	0.2	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	8	0.2	No Hit
GCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGA	8	0.2	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	8	0.2	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	8	0.2	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	8	0.2	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	8	0.2	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	8	0.2	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	8	0.2	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	8	0.2	No Hit
AAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACAC	8	0.2	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	8	0.2	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	8	0.2	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	7	0.17500000000000002	No Hit
TTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGGCAATAA	7	0.17500000000000002	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	7	0.17500000000000002	No Hit
GTCAATTTCTACATCTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGAC	7	0.17500000000000002	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	7	0.17500000000000002	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	7	0.17500000000000002	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	7	0.17500000000000002	No Hit
ATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCT	7	0.17500000000000002	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	7	0.17500000000000002	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	7	0.17500000000000002	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	7	0.17500000000000002	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	7	0.17500000000000002	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	7	0.17500000000000002	No Hit
TGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAAATTAAAT	7	0.17500000000000002	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	7	0.17500000000000002	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	7	0.17500000000000002	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	7	0.17500000000000002	No Hit
TTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTT	7	0.17500000000000002	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	7	0.17500000000000002	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	7	0.17500000000000002	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	7	0.17500000000000002	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	7	0.17500000000000002	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	7	0.17500000000000002	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	7	0.17500000000000002	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	7	0.17500000000000002	No Hit
TTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTG	6	0.15	No Hit
GTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATG	6	0.15	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	6	0.15	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	6	0.15	No Hit
CTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCA	6	0.15	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	6	0.15	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	6	0.15	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	6	0.15	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	6	0.15	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	6	0.15	No Hit
TGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTAC	6	0.15	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	6	0.15	No Hit
CCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATC	6	0.15	No Hit
CTTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	6	0.15	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	6	0.15	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	6	0.15	No Hit
GTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACC	6	0.15	No Hit
CAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGAT	6	0.15	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	6	0.15	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	6	0.15	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	6	0.15	No Hit
GTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACAC	5	0.125	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	5	0.125	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	5	0.125	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	5	0.125	No Hit
GTTATTGTTGGCCTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAG	5	0.125	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	5	0.125	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	5	0.125	No Hit
TTTAAATATTAAAAGGGTCCTTTCTGGCCTTCATACACATAAGTTTTAAA	5	0.125	No Hit
CCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAAT	5	0.125	No Hit
ACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAA	5	0.125	No Hit
TGGTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTAC	5	0.125	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	5	0.125	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	5	0.125	No Hit
ATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACATATGCAGTATG	5	0.125	No Hit
GTGCGTTTCCCTGTGTAGCCAGGGCTGCTCTGGACATCAGGATCTGCCTG	5	0.125	No Hit
CGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAAC	5	0.125	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	5	0.125	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	5	0.125	No Hit
TACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAA	5	0.125	No Hit
AATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTT	5	0.125	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	5	0.125	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	5	0.125	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	5	0.125	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	5	0.125	No Hit
AAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACA	5	0.125	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	5	0.125	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	5	0.125	No Hit
GTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTG	5	0.125	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	5	0.125	No Hit
TTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCC	5	0.125	No Hit
GATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATT	5	0.125	No Hit
GAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTC	5	0.125	No Hit
GGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.0625	0.0	0.0	0.0	0.0
72-73	0.1375	0.0	0.0	0.0	0.0
74-75	0.175	0.0	0.0	0.0	0.0
76-77	0.2625	0.0	0.0	0.0	0.0
78-79	0.325	0.0	0.0	0.0	0.0
80-81	0.35	0.0	0.0	0.0	0.0
82-83	0.4	0.0	0.0	0.0	0.0
84-85	0.4375	0.0	0.0	0.0	0.0
86-87	0.575	0.0	0.0	0.0	0.0
88-89	0.6625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882419 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882419_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2545	37.0	37.0	37.0	37.0	37.0
2	36.2265	37.0	37.0	37.0	37.0	37.0
3	36.298	37.0	37.0	37.0	37.0	37.0
4	36.3485	37.0	37.0	37.0	37.0	37.0
5	36.4305	37.0	37.0	37.0	37.0	37.0
6	36.353	37.0	37.0	37.0	37.0	37.0
7	36.349	37.0	37.0	37.0	37.0	37.0
8	36.495	37.0	37.0	37.0	37.0	37.0
9	36.4265	37.0	37.0	37.0	37.0	37.0
10-11	36.36225	37.0	37.0	37.0	37.0	37.0
12-13	36.422	37.0	37.0	37.0	37.0	37.0
14-15	36.3655	37.0	37.0	37.0	37.0	37.0
16-17	36.31675	37.0	37.0	37.0	37.0	37.0
18-19	36.39225	37.0	37.0	37.0	37.0	37.0
20-21	36.2935	37.0	37.0	37.0	37.0	37.0
22-23	36.3195	37.0	37.0	37.0	37.0	37.0
24-25	36.27525	37.0	37.0	37.0	37.0	37.0
26-27	36.282	37.0	37.0	37.0	37.0	37.0
28-29	36.26525	37.0	37.0	37.0	37.0	37.0
30-31	36.30475	37.0	37.0	37.0	37.0	37.0
32-33	36.24225	37.0	37.0	37.0	37.0	37.0
34-35	36.23225	37.0	37.0	37.0	37.0	37.0
36-37	36.219750000000005	37.0	37.0	37.0	37.0	37.0
38-39	36.272999999999996	37.0	37.0	37.0	37.0	37.0
40-41	36.228	37.0	37.0	37.0	37.0	37.0
42-43	36.178749999999994	37.0	37.0	37.0	37.0	37.0
44-45	36.131249999999994	37.0	37.0	37.0	37.0	37.0
46-47	36.199749999999995	37.0	37.0	37.0	37.0	37.0
48-49	36.088750000000005	37.0	37.0	37.0	37.0	37.0
50-51	36.128249999999994	37.0	37.0	37.0	37.0	37.0
52-53	36.124	37.0	37.0	37.0	37.0	37.0
54-55	36.129999999999995	37.0	37.0	37.0	37.0	37.0
56-57	36.0245	37.0	37.0	37.0	37.0	37.0
58-59	36.0465	37.0	37.0	37.0	37.0	37.0
60-61	36.085499999999996	37.0	37.0	37.0	37.0	37.0
62-63	35.98	37.0	37.0	37.0	37.0	37.0
64-65	36.031	37.0	37.0	37.0	37.0	37.0
66-67	35.981	37.0	37.0	37.0	37.0	37.0
68-69	36.0145	37.0	37.0	37.0	37.0	37.0
70-71	35.946	37.0	37.0	37.0	37.0	37.0
72-73	35.971500000000006	37.0	37.0	37.0	37.0	37.0
74-75	36.016999999999996	37.0	37.0	37.0	37.0	37.0
76-77	35.935	37.0	37.0	37.0	37.0	37.0
78-79	35.96525	37.0	37.0	37.0	37.0	37.0
80-81	35.93075	37.0	37.0	37.0	37.0	37.0
82-83	35.856	37.0	37.0	37.0	37.0	37.0
84-85	35.80525	37.0	37.0	37.0	37.0	37.0
86-87	35.85375	37.0	37.0	37.0	37.0	37.0
88-89	35.799499999999995	37.0	37.0	37.0	37.0	37.0
90-91	35.795	37.0	37.0	37.0	37.0	37.0
92-93	35.76675	37.0	37.0	37.0	37.0	37.0
94-95	35.7805	37.0	37.0	37.0	37.0	37.0
96-97	35.756	37.0	37.0	37.0	37.0	37.0
98-99	35.74275	37.0	37.0	37.0	37.0	37.0
100-101	35.39425	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	2.0
23	1.0
24	5.0
25	5.0
26	8.0
27	13.0
28	12.0
29	19.0
30	21.0
31	34.0
32	47.0
33	67.0
34	128.0
35	506.0
36	2762.0
37	369.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.35087719298245	20.20050125313283	14.210526315789473	25.238095238095237
2	19.900000000000002	27.400000000000002	33.45	19.25
3	20.599999999999998	26.85	31.874999999999996	20.674999999999997
4	26.375	33.5	21.4	18.725
5	26.125	33.275	22.825	17.775
6	22.25	36.4	22.3	19.05
7	20.7	21.55	38.9	18.85
8	23.175	22.3	29.299999999999997	25.224999999999998
9	23.1	24.925	28.799999999999997	23.175
10-11	24.825	32.875	22.237499999999997	20.0625
12-13	25.85	24.075	27.0875	22.9875
14-15	23.825	26.275	29.675	20.225
16-17	24.962500000000002	27.875	28.5625	18.6
18-19	21.975	28.475	29.2375	20.3125
20-21	22.975	30.0	27.250000000000004	19.775000000000002
22-23	22.2	30.012499999999996	26.575	21.212500000000002
24-25	24.775	27.5875	26.400000000000002	21.2375
26-27	23.6875	27.025	27.762500000000003	21.525
28-29	24.6	27.500000000000004	26.487500000000004	21.4125
30-31	24.224999999999998	27.3375	27.950000000000003	20.4875
32-33	23.0375	27.8625	27.987499999999997	21.1125
34-35	23.0	26.6125	27.487499999999997	22.900000000000002
36-37	22.95	26.8375	28.812500000000004	21.4
38-39	23.375	26.487500000000004	27.474999999999998	22.662499999999998
40-41	22.05	29.3875	27.375	21.1875
42-43	23.7625	28.6125	27.6625	19.9625
44-45	22.9875	28.3125	29.375	19.325
46-47	23.8875	26.787499999999998	25.4	23.925
48-49	23.025000000000002	28.1	28.812500000000004	20.0625
50-51	22.925	28.0625	26.6625	22.35
52-53	24.099999999999998	26.85	28.7	20.349999999999998
54-55	24.7375	27.0125	26.5375	21.712500000000002
56-57	21.6125	29.262500000000003	26.9625	22.162499999999998
58-59	21.7	27.3875	27.3	23.6125
60-61	21.9625	27.1	28.0625	22.875
62-63	22.9625	28.9875	26.0	22.05
64-65	22.775000000000002	27.5125	29.625	20.0875
66-67	22.0625	25.55	29.425	22.9625
68-69	23.3125	29.349999999999998	26.174999999999997	21.1625
70-71	22.0	30.099999999999998	27.025	20.875
72-73	22.8625	28.1875	27.250000000000004	21.7
74-75	23.9	27.0625	26.8125	22.225
76-77	22.225	28.4125	26.787499999999998	22.575
78-79	23.1125	27.875	27.237499999999997	21.775
80-81	23.25	27.500000000000004	28.375	20.875
82-83	22.9375	27.224999999999998	27.525	22.3125
84-85	20.7875	26.85	29.325000000000003	23.0375
86-87	22.85	28.6125	25.900000000000002	22.6375
88-89	23.974999999999998	27.8375	26.8625	21.325
90-91	24.4125	27.900000000000002	26.1125	21.575
92-93	22.75	30.975	27.35	18.925
94-95	22.6125	31.5	25.275	20.6125
96-97	23.150000000000002	28.1875	27.075	21.587500000000002
98-99	22.5875	28.7375	26.025	22.650000000000002
100-101	24.4	28.175	26.224999999999998	21.2
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	2.0
25	3.5
26	2.0
27	2.0
28	6.0
29	10.5
30	34.0
31	117.0
32	158.5
33	108.5
34	97.5
35	100.0
36	96.0
37	105.0
38	91.5
39	90.5
40	129.0
41	175.0
42	203.5
43	211.5
44	199.0
45	208.0
46	206.5
47	190.5
48	175.5
49	142.5
50	120.5
51	118.0
52	106.5
53	90.5
54	84.0
55	111.5
56	100.5
57	55.0
58	61.5
59	63.5
60	47.5
61	31.5
62	27.5
63	17.5
64	4.0
65	1.5
66	2.5
67	1.5
68	1.0
69	7.0
70	9.0
71	7.5
72	10.5
73	8.0
74	6.5
75	6.5
76	8.0
77	12.5
78	7.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.400000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.21946564885496	34.175
2	18.654580152671755	19.55
3	5.916030534351145	9.3
4	2.8625954198473282	6.0
5	2.051526717557252	5.375
6	1.4790076335877862	4.65
7	1.1450381679389312	4.2
8	0.9064885496183206	3.8
9	0.33396946564885494	1.575
>10	1.4312977099236641	11.375
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	40	1.0	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	20	0.5	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	19	0.475	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	18	0.44999999999999996	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	17	0.42500000000000004	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	16	0.4	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	16	0.4	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	16	0.4	No Hit
GCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCC	16	0.4	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	16	0.4	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	16	0.4	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	15	0.375	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	15	0.375	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	15	0.375	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	15	0.375	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	15	0.375	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	14	0.35000000000000003	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	14	0.35000000000000003	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	14	0.35000000000000003	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	14	0.35000000000000003	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	13	0.325	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	13	0.325	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	12	0.3	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	12	0.3	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	12	0.3	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	11	0.27499999999999997	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	11	0.27499999999999997	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	10	0.25	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	10	0.25	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	10	0.25	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	9	0.22499999999999998	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	9	0.22499999999999998	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	9	0.22499999999999998	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	9	0.22499999999999998	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	9	0.22499999999999998	No Hit
GAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGG	9	0.22499999999999998	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	9	0.22499999999999998	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	8	0.2	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	8	0.2	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	8	0.2	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	8	0.2	No Hit
GCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAA	8	0.2	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	8	0.2	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	8	0.2	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	8	0.2	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	8	0.2	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	8	0.2	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	8	0.2	No Hit
CTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGT	8	0.2	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	8	0.2	No Hit
GTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGT	8	0.2	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	8	0.2	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	8	0.2	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	8	0.2	No Hit
CCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACC	8	0.2	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	8	0.2	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	7	0.17500000000000002	No Hit
AAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCT	7	0.17500000000000002	No Hit
AAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAG	7	0.17500000000000002	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	7	0.17500000000000002	No Hit
GGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTC	7	0.17500000000000002	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	7	0.17500000000000002	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	7	0.17500000000000002	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	7	0.17500000000000002	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	7	0.17500000000000002	No Hit
GATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGG	7	0.17500000000000002	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	7	0.17500000000000002	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	7	0.17500000000000002	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	7	0.17500000000000002	No Hit
GCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGACTCGGCGCCG	7	0.17500000000000002	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	7	0.17500000000000002	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	7	0.17500000000000002	No Hit
GTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATT	7	0.17500000000000002	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	7	0.17500000000000002	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	7	0.17500000000000002	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	7	0.17500000000000002	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	7	0.17500000000000002	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	7	0.17500000000000002	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	7	0.17500000000000002	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	7	0.17500000000000002	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	6	0.15	No Hit
GAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAG	6	0.15	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	6	0.15	No Hit
GTTGAAAGTGCCAGTGTCAAAGAAAGTGTTGAGAAGCTGCCTCTTCAGTG	6	0.15	No Hit
AACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGC	6	0.15	No Hit
TAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTAC	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
GTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAG	6	0.15	No Hit
ATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCAA	6	0.15	No Hit
GACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCT	6	0.15	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	6	0.15	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	6	0.15	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	6	0.15	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	6	0.15	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	6	0.15	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	6	0.15	No Hit
CTGGTGACTCTTACTCTACCCTCTAAGTCAAGGGGCTGTCAGTGCGCAGG	6	0.15	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	6	0.15	No Hit
GGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTG	6	0.15	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	6	0.15	No Hit
AATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAAT	6	0.15	No Hit
ATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAA	6	0.15	No Hit
CACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAA	6	0.15	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	6	0.15	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	6	0.15	No Hit
GAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGA	6	0.15	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	6	0.15	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	6	0.15	No Hit
GGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGG	6	0.15	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	6	0.15	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	6	0.15	No Hit
GTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACA	5	0.125	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	5	0.125	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	5	0.125	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	5	0.125	No Hit
CTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTG	5	0.125	No Hit
AAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTG	5	0.125	No Hit
GCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGT	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
TGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGT	5	0.125	No Hit
TGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGAC	5	0.125	No Hit
GTAACTCCTGCTCCTGAAAGCATCCTACATTCTGACCTTCAAAGAGAAGT	5	0.125	No Hit
CTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCAT	5	0.125	No Hit
TGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAAT	5	0.125	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	5	0.125	No Hit
TCTTGTTCACCACTTCAAGGATTATCAGCCTTTGGTTCATGAATGAGAAG	5	0.125	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	5	0.125	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	5	0.125	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	5	0.125	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	5	0.125	No Hit
TGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTAC	5	0.125	No Hit
AGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGA	5	0.125	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	5	0.125	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	5	0.125	No Hit
GTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTG	5	0.125	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	5	0.125	No Hit
TTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTG	5	0.125	No Hit
AAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGC	5	0.125	No Hit
GGTGGTGACTGGCGGCGGTGAGGCGGCCGGTGGCGGCGGCGGCGGCGGTG	5	0.125	No Hit
TTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATA	5	0.125	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	5	0.125	No Hit
CTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCC	5	0.125	No Hit
CCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGAC	5	0.125	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	5	0.125	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	5	0.125	No Hit
AAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAA	5	0.125	No Hit
TTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTG	5	0.125	No Hit
ACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGA	5	0.125	No Hit
GGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAA	5	0.125	No Hit
CCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTAC	5	0.125	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	5	0.125	No Hit
CAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTA	5	0.125	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	5	0.125	No Hit
CATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.0625	0.0	0.0	0.0	0.0
72-73	0.1375	0.0	0.0	0.0	0.0
74-75	0.175	0.0	0.0	0.0	0.0
76-77	0.2625	0.0	0.0	0.0	0.0
78-79	0.325	0.0	0.0	0.0	0.0
80-81	0.325	0.0	0.0	0.0	0.0
82-83	0.375	0.0	0.0	0.0	0.0
84-85	0.4125	0.0	0.0	0.0	0.0
86-87	0.55	0.0	0.0	0.0	0.0
88-89	0.6375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGGT	20	0.0019257745	71.25	7
ATGGTCA	20	0.0019257745	71.25	9
TTCTTAT	20	0.0019257745	71.25	4
CTTATGG	20	0.0019257745	71.25	6
TATGGTC	20	0.0019257745	71.25	8
TCTTATG	25	0.0046641747	57.0	5
TTTTCTT	30	0.009594663	47.5	2
>>END_MODULE
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053806 spots for SRR24882419.sra
Written 2053806 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
Read 2053805 spots for SRR24882419.sra
Written 2053805 spots for SRR24882419.sra
SRR ids: ['SRR24882419.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_513xzvrp
SRR24882419.sra spots: 32860881
blocks: [[1, 2053805], [2053806, 4107610], [4107611, 6161415], [6161416, 8215220], [8215221, 10269025], [10269026, 12322830], [12322831, 14376635], [14376636, 16430440], [16430441, 18484245], [18484246, 20538050], [20538051, 22591855], [22591856, 24645660], [24645661, 26699465], [26699466, 28753270], [28753271, 30807075], [30807076, 32860881]]
SRR24882419 file size 965201
SRR24882419 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24882419 SRR24882419_1.fastq SRR24882419_2.fastq
Input file:	SRR24882419_1.fastq
Paired file:	SRR24882419_2.fastq
trimmed:	SRR24882419-trimmed-pair1.fastq, SRR24882419-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 06:15:15 2026 >> started

Fri Jul  3 06:16:11 2026 >> done (56.476s)
32860881 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
32860881 (100.00%) read pairs available; of these:
  719391 ( 2.19%) trimmed read pairs available after processing
32141490 (97.81%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 64	       1	  0.00%
 65	       1	  0.00%
 66	      23	  0.00%
 67	     994	  0.00%
 68	    5174	  0.02%
 69	    6989	  0.02%
 70	    8122	  0.02%
 71	    8724	  0.03%
 72	    9895	  0.03%
 73	   10226	  0.03%
 74	   10687	  0.03%
 75	   11822	  0.04%
 76	   12572	  0.04%
 77	   13834	  0.04%
 78	   13901	  0.04%
 79	   14830	  0.05%
 80	   15339	  0.05%
 81	   16617	  0.05%
 82	   17337	  0.05%
 83	   18237	  0.06%
 84	   19278	  0.06%
 85	   20335	  0.06%
 86	   20544	  0.06%
 87	   21919	  0.07%
 88	   23625	  0.07%
 89	   23891	  0.07%
 90	   26272	  0.08%
 91	   28055	  0.09%
 92	   28368	  0.09%
 93	   29897	  0.09%
 94	   31186	  0.09%
 95	   33056	  0.10%
 96	   33986	  0.10%
 97	   37043	  0.11%
 98	   37267	  0.11%
 99	   41254	  0.13%
100	   68090	  0.21%
101	32141490	 97.81%
32860881 reads passed initial QC


criterion=sequence-density
sequence-density=1.10
sequence-density-rank=1
fanout-score=2.76
fanout-score-rank=27
prefix-density=1.26
prefix-fanout=2.4
sequence=GATGGAGAAGATG


criterion=fanout-score
sequence-density=0.20
sequence-density-rank=23
fanout-score=44.98
fanout-score-rank=1
prefix-density=1.97
prefix-fanout=4.5
sequence=CTTCTTCTTGGC


criterion=sequence-density
sequence-density=1.17
sequence-density-rank=1
fanout-score=2.06
fanout-score-rank=35
prefix-density=1.16
prefix-fanout=2.1
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.24
sequence-density-rank=16
fanout-score=25.73
fanout-score-rank=1
prefix-density=1.02
prefix-fanout=6.1
sequence=GAGGAGCTGGAGGAGG
SRR24882419 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 06:16:56
                             Started mapping on |	Jul 03 06:16:56
                                    Finished on |	Jul 03 06:55:23
       Mapping speed, Million of reads per hour |	51.28

                          Number of input reads |	32860881
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	28957203
                        Uniquely mapped reads % |	88.12%
                          Average mapped length |	200.33
                       Number of splices: Total |	18670382
            Number of splices: Annotated (sjdb) |	18518497
                       Number of splices: GT/AG |	18529022
                       Number of splices: GC/AG |	109923
                       Number of splices: AT/AC |	11389
               Number of splices: Non-canonical |	20048
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.03%
                        Deletion average length |	4.01
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.72
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3895619
             % of reads mapped to multiple loci |	11.85%
        Number of reads mapped to too many loci |	2979
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8059	8059	8059
N_multimapping	3895619	3895619	3895619
N_noFeature	2722315	25160524	3759354
N_ambiguous	3026714	1418989	254367
UnstrandedReadsAssigned:23208174 PositiveStrandReadsAssigned:2377690 NegativeStrandReadsAssigned:24943482
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882419 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882419-trimmed-pair1.fastq
                             SRR24882419-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 32,860,881 reads, 26,141,194 reads pseudoaligned
[quant] estimated average fragment length: 200.754
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,235 rounds

 109283 SRR24882419.ke.tsv
  52636 SRR24882419.se.tsv
 161919 total
==> SRR24882419.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7.75	0	0
ENSMUST00000196221.1	9	5	0	0
ENSMUST00000179664.1	11	7	0	0
ENSMUST00000178537.1	12	5.66667	0	0
ENSMUST00000178862.1	14	7.66667	0	0
ENSMUST00000179520.1	11	7	0	0
ENSMUST00000179883.1	16	7.75	0	0
ENSMUST00000195858.1	10	6	0	0
ENSMUST00000179932.1	12	5.66667	0	0

==> SRR24882419.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	4
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882419 completed mapping pipeline successfully
