Starting /dee2/code/volunteer_pipeline.sh SRR24882417
    current disk space = 1385110252
    free memory = 197745972 
SRR24882417 SRAfilesize
3cd4f0a3962d24a33678ed68607ba4b7  SRR24882417.sra
SRR24882417.sra file validated
SRR24882417 is paired end
SRR24882417 is conventional basespace
SRR24882417 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.598	37.0	37.0	37.0	37.0	37.0
2	36.51625	37.0	37.0	37.0	37.0	37.0
3	36.6	37.0	37.0	37.0	37.0	37.0
4	36.629	37.0	37.0	37.0	37.0	37.0
5	36.6955	37.0	37.0	37.0	37.0	37.0
6	36.6825	37.0	37.0	37.0	37.0	37.0
7	36.615	37.0	37.0	37.0	37.0	37.0
8	36.6015	37.0	37.0	37.0	37.0	37.0
9	36.6605	37.0	37.0	37.0	37.0	37.0
10-11	36.60425	37.0	37.0	37.0	37.0	37.0
12-13	36.64475	37.0	37.0	37.0	37.0	37.0
14-15	36.62325	37.0	37.0	37.0	37.0	37.0
16-17	36.61725	37.0	37.0	37.0	37.0	37.0
18-19	36.636250000000004	37.0	37.0	37.0	37.0	37.0
20-21	36.55025	37.0	37.0	37.0	37.0	37.0
22-23	36.56075	37.0	37.0	37.0	37.0	37.0
24-25	36.594	37.0	37.0	37.0	37.0	37.0
26-27	36.528999999999996	37.0	37.0	37.0	37.0	37.0
28-29	36.570750000000004	37.0	37.0	37.0	37.0	37.0
30-31	36.52475	37.0	37.0	37.0	37.0	37.0
32-33	36.45225	37.0	37.0	37.0	37.0	37.0
34-35	36.50175	37.0	37.0	37.0	37.0	37.0
36-37	36.484	37.0	37.0	37.0	37.0	37.0
38-39	36.48075	37.0	37.0	37.0	37.0	37.0
40-41	36.537	37.0	37.0	37.0	37.0	37.0
42-43	36.48625	37.0	37.0	37.0	37.0	37.0
44-45	36.5115	37.0	37.0	37.0	37.0	37.0
46-47	36.49325	37.0	37.0	37.0	37.0	37.0
48-49	36.44725	37.0	37.0	37.0	37.0	37.0
50-51	36.491749999999996	37.0	37.0	37.0	37.0	37.0
52-53	36.4815	37.0	37.0	37.0	37.0	37.0
54-55	36.426249999999996	37.0	37.0	37.0	37.0	37.0
56-57	36.47	37.0	37.0	37.0	37.0	37.0
58-59	36.464749999999995	37.0	37.0	37.0	37.0	37.0
60-61	36.480500000000006	37.0	37.0	37.0	37.0	37.0
62-63	36.43675	37.0	37.0	37.0	37.0	37.0
64-65	36.395	37.0	37.0	37.0	37.0	37.0
66-67	36.403000000000006	37.0	37.0	37.0	37.0	37.0
68-69	36.386250000000004	37.0	37.0	37.0	37.0	37.0
70-71	36.31725	37.0	37.0	37.0	37.0	37.0
72-73	36.397	37.0	37.0	37.0	37.0	37.0
74-75	36.28875	37.0	37.0	37.0	37.0	37.0
76-77	36.376000000000005	37.0	37.0	37.0	37.0	37.0
78-79	36.25725	37.0	37.0	37.0	37.0	37.0
80-81	36.257000000000005	37.0	37.0	37.0	37.0	37.0
82-83	36.286	37.0	37.0	37.0	37.0	37.0
84-85	36.208	37.0	37.0	37.0	37.0	37.0
86-87	36.21975	37.0	37.0	37.0	37.0	37.0
88-89	36.293	37.0	37.0	37.0	37.0	37.0
90-91	36.317	37.0	37.0	37.0	37.0	37.0
92-93	36.27775	37.0	37.0	37.0	37.0	37.0
94-95	36.15525	37.0	37.0	37.0	37.0	37.0
96-97	36.08975	37.0	37.0	37.0	37.0	37.0
98-99	36.01	37.0	37.0	37.0	37.0	37.0
100-101	36.00575	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	0.0
26	2.0
27	5.0
28	7.0
29	13.0
30	18.0
31	24.0
32	36.0
33	56.0
34	90.0
35	227.0
36	2640.0
37	880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.5	10.8	13.350000000000001	39.35
2	15.928982245561391	17.67941985496374	38.834708677169296	27.556889222305575
3	18.543543543543546	20.245245245245243	31.431431431431434	29.77977977977978
4	24.525	28.225	23.549999999999997	23.7
5	22.175	31.874999999999996	27.125	18.825
6	20.150000000000002	33.725	28.125	18.0
7	14.899999999999999	24.3	44.55	16.25
8	20.974999999999998	23.150000000000002	31.025000000000002	24.85
9	18.8	24.725	32.6	23.875
10-11	20.2375	31.974999999999998	26.424999999999997	21.3625
12-13	20.7	25.4	28.275	25.624999999999996
14-15	19.6	28.537499999999998	29.099999999999998	22.7625
16-17	19.5125	29.812499999999996	26.3	24.375
18-19	22.0125	30.75	25.4625	21.775
20-21	20.837500000000002	29.65	27.474999999999998	22.037499999999998
22-23	22.7	27.3875	29.1375	20.775
24-25	22.5875	30.9	24.837500000000002	21.675
26-27	21.25	31.2875	26.5375	20.925
28-29	19.7375	29.6375	27.525	23.1
30-31	20.849999999999998	27.675	28.349999999999998	23.125
32-33	19.4875	26.937499999999996	30.6375	22.9375
34-35	18.45	30.725	27.6375	23.1875
36-37	20.575	28.3375	29.799999999999997	21.2875
38-39	19.425	29.562500000000004	30.675	20.3375
40-41	20.95	29.8875	29.75	19.412499999999998
42-43	20.225	31.324999999999996	27.625	20.825
44-45	18.625	31.412499999999998	26.474999999999998	23.4875
46-47	21.0375	31.137500000000003	25.137500000000003	22.6875
48-49	20.9875	31.1	25.874999999999996	22.037499999999998
50-51	21.2	29.325000000000003	27.762500000000003	21.712500000000002
52-53	21.6125	27.787499999999998	27.3125	23.2875
54-55	20.5	27.450000000000003	28.7375	23.3125
56-57	19.0875	27.762500000000003	29.6625	23.4875
58-59	19.2625	27.6125	30.062499999999996	23.0625
60-61	19.112499999999997	30.65	27.650000000000002	22.5875
62-63	21.5625	28.3625	28.1625	21.912499999999998
64-65	22.325	26.4625	27.900000000000002	23.3125
66-67	21.337500000000002	26.0375	29.7125	22.912499999999998
68-69	20.9125	27.987499999999997	27.0875	24.0125
70-71	21.575	27.925	26.787499999999998	23.7125
72-73	20.775	28.8875	27.6125	22.725
74-75	21.2375	28.487499999999997	28.725	21.55
76-77	21.8625	29.099999999999998	27.325	21.712500000000002
78-79	20.25	28.175	29.225	22.35
80-81	22.25	27.8125	27.3875	22.55
82-83	20.1	30.099999999999998	29.125	20.674999999999997
84-85	19.2625	29.7875	27.9125	23.0375
86-87	20.3375	30.887500000000003	26.875	21.9
88-89	23.175	26.575	29.0875	21.1625
90-91	21.075	28.962500000000002	27.5625	22.400000000000002
92-93	21.8125	28.825	28.1	21.2625
94-95	20.474999999999998	30.825000000000003	27.125	21.575
96-97	20.6375	30.4375	24.349999999999998	24.575
98-99	18.987499999999997	31.0	28.025	21.987499999999997
100-101	20.275000000000002	29.062500000000004	29.5375	21.125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.5
20	0.5
21	1.0
22	1.0
23	0.5
24	1.0
25	1.0
26	2.0
27	4.0
28	4.0
29	14.5
30	35.5
31	70.5
32	138.0
33	161.5
34	129.5
35	120.5
36	115.5
37	107.0
38	77.5
39	71.5
40	117.5
41	195.5
42	278.5
43	257.0
44	209.5
45	210.5
46	260.5
47	308.5
48	237.5
49	151.5
50	148.0
51	130.5
52	77.0
53	62.5
54	68.5
55	47.5
56	20.0
57	14.0
58	13.5
59	7.5
60	8.0
61	6.0
62	7.5
63	8.5
64	4.0
65	5.0
66	7.0
67	10.5
68	11.5
69	7.5
70	4.0
71	3.0
72	3.5
73	4.0
74	6.5
75	8.0
76	6.0
77	6.0
78	5.0
79	3.0
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.1
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.66591166477916	28.549999999999997
2	16.25141562853907	14.35
3	5.889014722536806	7.8
4	3.454133635334088	6.1
5	1.7553793884484712	3.875
6	1.5288788221970555	4.05
7	1.189127972819932	3.675
8	0.7361268403171007	2.6
9	0.6795016987542469	2.7
>10	3.8505096262740657	26.3
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	39	0.975	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	33	0.8250000000000001	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	28	0.7000000000000001	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	28	0.7000000000000001	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	28	0.7000000000000001	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	25	0.625	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	25	0.625	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	24	0.6	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	23	0.575	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	22	0.5499999999999999	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	21	0.525	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	20	0.5	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	20	0.5	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	19	0.475	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	19	0.475	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	19	0.475	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	19	0.475	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	17	0.42500000000000004	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	17	0.42500000000000004	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	17	0.42500000000000004	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	17	0.42500000000000004	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	16	0.4	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	16	0.4	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	16	0.4	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	16	0.4	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	15	0.375	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	15	0.375	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	15	0.375	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	14	0.35000000000000003	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	14	0.35000000000000003	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	14	0.35000000000000003	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	14	0.35000000000000003	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	14	0.35000000000000003	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	13	0.325	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	13	0.325	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	13	0.325	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	13	0.325	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	13	0.325	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	12	0.3	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	12	0.3	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	12	0.3	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	12	0.3	No Hit
CACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTT	12	0.3	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	12	0.3	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	12	0.3	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	12	0.3	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	12	0.3	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	11	0.27499999999999997	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	11	0.27499999999999997	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	11	0.27499999999999997	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	11	0.27499999999999997	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	11	0.27499999999999997	No Hit
TTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTG	11	0.27499999999999997	No Hit
GAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATT	11	0.27499999999999997	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	11	0.27499999999999997	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	11	0.27499999999999997	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	11	0.27499999999999997	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	10	0.25	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	10	0.25	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	10	0.25	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	10	0.25	No Hit
ATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTC	10	0.25	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	10	0.25	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	10	0.25	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	10	0.25	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	10	0.25	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	10	0.25	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	10	0.25	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	9	0.22499999999999998	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	9	0.22499999999999998	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	9	0.22499999999999998	No Hit
CCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGT	9	0.22499999999999998	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	9	0.22499999999999998	No Hit
GGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGA	9	0.22499999999999998	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	9	0.22499999999999998	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	9	0.22499999999999998	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	9	0.22499999999999998	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	9	0.22499999999999998	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	9	0.22499999999999998	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	9	0.22499999999999998	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	8	0.2	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	8	0.2	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	8	0.2	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	8	0.2	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	8	0.2	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	8	0.2	No Hit
GAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGA	8	0.2	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	8	0.2	No Hit
CGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGG	8	0.2	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	8	0.2	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	8	0.2	No Hit
GCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTT	8	0.2	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	8	0.2	No Hit
CACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTAT	7	0.17500000000000002	No Hit
TGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCT	7	0.17500000000000002	No Hit
CTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCT	7	0.17500000000000002	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	7	0.17500000000000002	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	7	0.17500000000000002	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	7	0.17500000000000002	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	7	0.17500000000000002	No Hit
GACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTG	7	0.17500000000000002	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	7	0.17500000000000002	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	7	0.17500000000000002	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	7	0.17500000000000002	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	7	0.17500000000000002	No Hit
CAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGAT	7	0.17500000000000002	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	7	0.17500000000000002	No Hit
TGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTAC	7	0.17500000000000002	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	7	0.17500000000000002	No Hit
GTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTG	7	0.17500000000000002	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	7	0.17500000000000002	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	7	0.17500000000000002	No Hit
CCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTGGC	7	0.17500000000000002	No Hit
CCTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATA	7	0.17500000000000002	No Hit
TCTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACAT	6	0.15	No Hit
TGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCCT	6	0.15	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	6	0.15	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	6	0.15	No Hit
CACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAG	6	0.15	No Hit
TTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAA	6	0.15	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	6	0.15	No Hit
CGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAAC	6	0.15	No Hit
GTCACAATCAGGACCATGAACCACTGAGAGAAACCAGAGATGAAACCAGA	6	0.15	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	6	0.15	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	6	0.15	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	6	0.15	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	6	0.15	No Hit
GTCCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATA	6	0.15	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	6	0.15	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	6	0.15	No Hit
TTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTT	6	0.15	No Hit
CACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTT	6	0.15	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	6	0.15	No Hit
ATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTT	6	0.15	No Hit
TCTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTC	6	0.15	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	6	0.15	No Hit
CAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATT	6	0.15	No Hit
GATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATT	6	0.15	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	6	0.15	No Hit
ATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACA	6	0.15	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	6	0.15	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	5	0.125	No Hit
GCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATT	5	0.125	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	5	0.125	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	5	0.125	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	5	0.125	No Hit
CCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGA	5	0.125	No Hit
CATTGGTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTAT	5	0.125	No Hit
CTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAG	5	0.125	No Hit
CTTTCCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTAC	5	0.125	No Hit
TTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACAT	5	0.125	No Hit
TACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAA	5	0.125	No Hit
CTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGT	5	0.125	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	5	0.125	No Hit
ATTGGTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATT	5	0.125	No Hit
ACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGG	5	0.125	No Hit
CCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGC	5	0.125	No Hit
CATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACT	5	0.125	No Hit
TGTAATTTCGAAGCTCTTTCCTCCTCGCTGTTGGCATCAAGAAGATCATC	5	0.125	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	5	0.125	No Hit
GAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCT	5	0.125	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	5	0.125	No Hit
CAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACACACACACA	5	0.125	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	5	0.125	No Hit
CCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCA	5	0.125	No Hit
GTCTACCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAA	5	0.125	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	5	0.125	No Hit
CTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCC	5	0.125	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	5	0.125	No Hit
GTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTCCTCCTCGCTGTTGG	5	0.125	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	5	0.125	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0125	0.0	0.0	0.0	0.0
76-77	0.025	0.0	0.0	0.0	0.0
78-79	0.075	0.0	0.0	0.0	0.0
80-81	0.1375	0.0	0.0	0.0	0.0
82-83	0.15	0.0	0.0	0.0	0.0
84-85	0.23750000000000002	0.0	0.0	0.0	0.0
86-87	0.375	0.0	0.0	0.0	0.0
88-89	0.475	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882417 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882417_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.326	37.0	37.0	37.0	37.0	37.0
2	36.3485	37.0	37.0	37.0	37.0	37.0
3	36.433	37.0	37.0	37.0	37.0	37.0
4	36.4525	37.0	37.0	37.0	37.0	37.0
5	36.5375	37.0	37.0	37.0	37.0	37.0
6	36.469	37.0	37.0	37.0	37.0	37.0
7	36.4915	37.0	37.0	37.0	37.0	37.0
8	36.4335	37.0	37.0	37.0	37.0	37.0
9	36.435	37.0	37.0	37.0	37.0	37.0
10-11	36.455749999999995	37.0	37.0	37.0	37.0	37.0
12-13	36.4735	37.0	37.0	37.0	37.0	37.0
14-15	36.5005	37.0	37.0	37.0	37.0	37.0
16-17	36.4755	37.0	37.0	37.0	37.0	37.0
18-19	36.43325	37.0	37.0	37.0	37.0	37.0
20-21	36.394000000000005	37.0	37.0	37.0	37.0	37.0
22-23	36.413	37.0	37.0	37.0	37.0	37.0
24-25	36.353	37.0	37.0	37.0	37.0	37.0
26-27	36.320750000000004	37.0	37.0	37.0	37.0	37.0
28-29	36.398250000000004	37.0	37.0	37.0	37.0	37.0
30-31	36.311499999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.341	37.0	37.0	37.0	37.0	37.0
34-35	36.35825	37.0	37.0	37.0	37.0	37.0
36-37	36.38075	37.0	37.0	37.0	37.0	37.0
38-39	36.292500000000004	37.0	37.0	37.0	37.0	37.0
40-41	36.2545	37.0	37.0	37.0	37.0	37.0
42-43	36.317750000000004	37.0	37.0	37.0	37.0	37.0
44-45	36.27475	37.0	37.0	37.0	37.0	37.0
46-47	36.24575	37.0	37.0	37.0	37.0	37.0
48-49	36.154	37.0	37.0	37.0	37.0	37.0
50-51	36.17575	37.0	37.0	37.0	37.0	37.0
52-53	36.20225	37.0	37.0	37.0	37.0	37.0
54-55	36.2035	37.0	37.0	37.0	37.0	37.0
56-57	36.16975	37.0	37.0	37.0	37.0	37.0
58-59	36.25075	37.0	37.0	37.0	37.0	37.0
60-61	36.099000000000004	37.0	37.0	37.0	37.0	37.0
62-63	36.072	37.0	37.0	37.0	37.0	37.0
64-65	36.05775	37.0	37.0	37.0	37.0	37.0
66-67	36.073	37.0	37.0	37.0	37.0	37.0
68-69	36.0595	37.0	37.0	37.0	37.0	37.0
70-71	35.963499999999996	37.0	37.0	37.0	37.0	37.0
72-73	35.98925	37.0	37.0	37.0	37.0	37.0
74-75	36.0405	37.0	37.0	37.0	37.0	37.0
76-77	36.05275	37.0	37.0	37.0	37.0	37.0
78-79	35.958749999999995	37.0	37.0	37.0	37.0	37.0
80-81	35.85625	37.0	37.0	37.0	37.0	37.0
82-83	35.87825	37.0	37.0	37.0	37.0	37.0
84-85	35.95675	37.0	37.0	37.0	37.0	37.0
86-87	35.92	37.0	37.0	37.0	37.0	37.0
88-89	35.7765	37.0	37.0	37.0	37.0	37.0
90-91	35.86150000000001	37.0	37.0	37.0	37.0	37.0
92-93	35.7535	37.0	37.0	37.0	37.0	37.0
94-95	35.817750000000004	37.0	37.0	37.0	37.0	37.0
96-97	35.823750000000004	37.0	37.0	37.0	37.0	37.0
98-99	35.8405	37.0	37.0	37.0	37.0	37.0
100-101	35.48225	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	0.0
24	2.0
25	4.0
26	4.0
27	5.0
28	9.0
29	20.0
30	24.0
31	27.0
32	44.0
33	63.0
34	138.0
35	492.0
36	2801.0
37	366.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.13026052104208	19.914829659318638	14.904809619238476	25.0501002004008
2	19.650000000000002	26.575	36.525	17.25
3	19.1	26.674999999999997	35.15	19.075
4	23.275000000000002	31.900000000000002	25.8	19.025
5	23.799999999999997	31.724999999999998	26.150000000000002	18.325
6	22.8	34.849999999999994	23.95	18.4
7	19.825	22.3	39.65	18.224999999999998
8	23.674999999999997	21.825	30.049999999999997	24.45
9	23.65	24.474999999999998	29.799999999999997	22.075
10-11	25.7625	30.6875	23.925	19.625
12-13	24.2875	25.1	28.025	22.5875
14-15	23.7375	25.825	31.275	19.162499999999998
16-17	22.775000000000002	27.987499999999997	31.4	17.837500000000002
18-19	23.275000000000002	28.9125	28.825	18.987499999999997
20-21	21.775	28.962500000000002	29.95	19.3125
22-23	23.5875	28.212500000000002	27.6875	20.5125
24-25	21.7875	28.812500000000004	28.4375	20.962500000000002
26-27	23.6875	27.900000000000002	29.2875	19.125
28-29	23.2625	26.187500000000004	29.075	21.475
30-31	25.424999999999997	25.825	30.3	18.45
32-33	23.225	27.474999999999998	29.625	19.675
34-35	22.912499999999998	28.199999999999996	28.075	20.8125
36-37	22.675	28.549999999999997	29.037499999999998	19.7375
38-39	23.474999999999998	26.6125	29.025000000000002	20.8875
40-41	20.6125	30.099999999999998	28.225	21.0625
42-43	22.287499999999998	30.312499999999996	28.425	18.975
44-45	23.4125	30.15	28.1875	18.25
46-47	23.325000000000003	28.9375	26.375	21.3625
48-49	22.8125	27.2625	30.225	19.7
50-51	22.575	28.925	28.425	20.075000000000003
52-53	22.45	28.95	28.6875	19.9125
54-55	24.587500000000002	28.375	27.6375	19.400000000000002
56-57	21.712500000000002	27.950000000000003	28.199999999999996	22.1375
58-59	21.5625	30.2	27.737499999999997	20.5
60-61	21.4375	29.5	27.5875	21.475
62-63	22.85	30.775000000000002	26.237500000000004	20.1375
64-65	24.0375	28.125	29.4375	18.4
66-67	21.9	26.325	29.7	22.075
68-69	22.5	28.475	28.849999999999998	20.175
70-71	19.9625	27.9125	31.825	20.3
72-73	22.15	29.599999999999998	27.075	21.175
74-75	23.7875	28.275	29.65	18.2875
76-77	22.2125	28.787499999999998	28.325	20.674999999999997
78-79	23.3875	27.212500000000002	29.1375	20.2625
80-81	22.5	27.1375	30.225	20.1375
82-83	21.075	26.825	31.900000000000002	20.200000000000003
84-85	20.599999999999998	25.974999999999998	31.6875	21.7375
86-87	22.75	28.462500000000002	28.1875	20.599999999999998
88-89	22.1375	26.924999999999997	30.112499999999997	20.825
90-91	23.6625	29.75	26.150000000000002	20.4375
92-93	21.525	30.362499999999997	27.650000000000002	20.4625
94-95	22.975	29.0875	29.15	18.787499999999998
96-97	23.575	28.037499999999998	28.3875	20.0
98-99	23.075000000000003	27.487499999999997	28.812500000000004	20.625
100-101	23.75	28.225	28.3625	19.662499999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	3.5
23	5.5
24	3.5
25	2.0
26	3.0
27	3.0
28	3.0
29	10.5
30	54.5
31	136.5
32	190.0
33	171.5
34	129.0
35	102.5
36	114.0
37	122.5
38	106.5
39	119.0
40	157.5
41	167.5
42	186.0
43	222.5
44	233.5
45	244.5
46	223.5
47	201.0
48	181.0
49	138.0
50	125.5
51	112.5
52	74.0
53	46.5
54	52.5
55	65.5
56	45.0
57	24.5
58	20.5
59	23.0
60	15.0
61	4.5
62	11.5
63	11.0
64	5.0
65	4.5
66	4.0
67	6.5
68	9.0
69	8.5
70	6.0
71	9.0
72	10.0
73	9.0
74	16.5
75	16.0
76	11.5
77	11.0
78	5.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.800000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.43526785714286	27.075
2	18.191964285714285	16.3
3	6.4732142857142865	8.7
4	3.962053571428571	7.1
5	3.013392857142857	6.75
6	2.232142857142857	6.0
7	1.171875	3.675
8	1.2834821428571428	4.6
9	0.6696428571428571	2.7
>10	2.5669642857142856	17.1
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	34	0.8500000000000001	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	32	0.8	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	29	0.7250000000000001	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	23	0.575	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	21	0.525	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	20	0.5	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	20	0.5	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	19	0.475	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	19	0.475	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	19	0.475	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	18	0.44999999999999996	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	18	0.44999999999999996	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	17	0.42500000000000004	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	16	0.4	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	16	0.4	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	15	0.375	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	14	0.35000000000000003	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	14	0.35000000000000003	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	14	0.35000000000000003	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	14	0.35000000000000003	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	13	0.325	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	13	0.325	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	13	0.325	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	13	0.325	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	13	0.325	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	12	0.3	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	12	0.3	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	12	0.3	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	12	0.3	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	12	0.3	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	12	0.3	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	11	0.27499999999999997	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	11	0.27499999999999997	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	11	0.27499999999999997	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	11	0.27499999999999997	No Hit
TAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAA	11	0.27499999999999997	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	10	0.25	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	10	0.25	No Hit
ATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCC	10	0.25	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	10	0.25	No Hit
AGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTA	10	0.25	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	10	0.25	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	10	0.25	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	10	0.25	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	10	0.25	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	10	0.25	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	9	0.22499999999999998	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	9	0.22499999999999998	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	9	0.22499999999999998	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	9	0.22499999999999998	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	9	0.22499999999999998	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	9	0.22499999999999998	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	9	0.22499999999999998	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	9	0.22499999999999998	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	9	0.22499999999999998	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	9	0.22499999999999998	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	9	0.22499999999999998	No Hit
TTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTC	9	0.22499999999999998	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	8	0.2	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	8	0.2	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	8	0.2	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	8	0.2	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	8	0.2	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	8	0.2	No Hit
GACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCT	8	0.2	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	8	0.2	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	8	0.2	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	8	0.2	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	8	0.2	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	8	0.2	No Hit
GAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGC	8	0.2	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	8	0.2	No Hit
ATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAA	8	0.2	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	8	0.2	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	8	0.2	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	8	0.2	No Hit
GTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTAC	8	0.2	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	8	0.2	No Hit
GATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAA	8	0.2	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	8	0.2	No Hit
GCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTGAAATACGACCGTA	8	0.2	No Hit
GAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAG	7	0.17500000000000002	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	7	0.17500000000000002	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	7	0.17500000000000002	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	7	0.17500000000000002	No Hit
GTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAA	7	0.17500000000000002	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	7	0.17500000000000002	No Hit
CTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCAT	7	0.17500000000000002	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	7	0.17500000000000002	No Hit
CTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAG	7	0.17500000000000002	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	7	0.17500000000000002	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	7	0.17500000000000002	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	7	0.17500000000000002	No Hit
GAAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGT	7	0.17500000000000002	No Hit
AATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTT	7	0.17500000000000002	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	7	0.17500000000000002	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	7	0.17500000000000002	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	7	0.17500000000000002	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	7	0.17500000000000002	No Hit
TTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTC	7	0.17500000000000002	No Hit
GGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGG	7	0.17500000000000002	No Hit
GTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGAT	7	0.17500000000000002	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	6	0.15	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	6	0.15	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	6	0.15	No Hit
TCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAAT	6	0.15	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	6	0.15	No Hit
GTGTAATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAAC	6	0.15	No Hit
ATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTGAGCT	6	0.15	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	6	0.15	No Hit
AGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAA	6	0.15	No Hit
ATGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACA	6	0.15	No Hit
CTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGC	6	0.15	No Hit
GATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGC	6	0.15	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	6	0.15	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	6	0.15	No Hit
AGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACA	6	0.15	No Hit
TTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACA	6	0.15	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	6	0.15	No Hit
TTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGAT	6	0.15	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	6	0.15	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	6	0.15	No Hit
CACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCC	6	0.15	No Hit
GGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGG	6	0.15	No Hit
ACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAAT	6	0.15	No Hit
CTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTCTC	6	0.15	No Hit
CAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGT	6	0.15	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	6	0.15	No Hit
GTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTG	6	0.15	No Hit
CCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGC	6	0.15	No Hit
TTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATG	6	0.15	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	6	0.15	No Hit
CACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAA	6	0.15	No Hit
GTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTAC	6	0.15	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	6	0.15	No Hit
GTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTT	6	0.15	No Hit
ATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGATGTG	6	0.15	No Hit
GGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGAC	6	0.15	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	6	0.15	No Hit
TATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTC	6	0.15	No Hit
GAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTG	6	0.15	No Hit
CAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAAT	6	0.15	No Hit
AAGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGG	5	0.125	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	5	0.125	No Hit
TCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTT	5	0.125	No Hit
ATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACA	5	0.125	No Hit
GAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAG	5	0.125	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	5	0.125	No Hit
CTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGT	5	0.125	No Hit
ATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACT	5	0.125	No Hit
ATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAAT	5	0.125	No Hit
CTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGA	5	0.125	No Hit
ATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCT	5	0.125	No Hit
CTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTC	5	0.125	No Hit
GGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTC	5	0.125	No Hit
AAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAG	5	0.125	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	5	0.125	No Hit
GCCTGATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATC	5	0.125	No Hit
GCGGTGGAACCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCA	5	0.125	No Hit
AGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTA	5	0.125	No Hit
CTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTG	5	0.125	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	5	0.125	No Hit
CCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCA	5	0.125	No Hit
TCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTT	5	0.125	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	5	0.125	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	5	0.125	No Hit
CTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGT	5	0.125	No Hit
CAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATT	5	0.125	No Hit
GTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGG	5	0.125	No Hit
AGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGT	5	0.125	No Hit
GTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTG	5	0.125	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	5	0.125	No Hit
CATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCT	5	0.125	No Hit
ATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGT	5	0.125	No Hit
GTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGG	5	0.125	No Hit
CTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCC	5	0.125	No Hit
AATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGA	5	0.125	No Hit
TTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATA	5	0.125	No Hit
AGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAA	5	0.125	No Hit
AATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGT	5	0.125	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	5	0.125	No Hit
ATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTG	5	0.125	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	5	0.125	No Hit
AAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAG	5	0.125	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	5	0.125	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	5	0.125	No Hit
GTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAA	5	0.125	No Hit
ATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTCC	5	0.125	No Hit
TCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTA	5	0.125	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	5	0.125	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	5	0.125	No Hit
CTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAG	5	0.125	No Hit
GCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAAC	5	0.125	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	5	0.125	No Hit
GCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCAT	5	0.125	No Hit
CATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.025	0.0	0.0	0.0	0.0
76-77	0.05	0.0	0.0	0.0	0.0
78-79	0.1	0.0	0.0	0.0	0.0
80-81	0.16249999999999998	0.0	0.0	0.0	0.0
82-83	0.175	0.0	0.0	0.0	0.0
84-85	0.2625	0.0	0.0	0.0	0.0
86-87	0.4	0.0	0.0	0.0	0.0
88-89	0.5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
Read 4154043 spots for SRR24882417.sra
Written 4154043 spots for SRR24882417.sra
Read 4154037 spots for SRR24882417.sra
Written 4154037 spots for SRR24882417.sra
SRR ids: ['SRR24882417.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ys0burkn
SRR24882417.sra spots: 33232302
blocks: [[1, 4154037], [4154038, 8308074], [8308075, 12462111], [12462112, 16616148], [16616149, 20770185], [20770186, 24924222], [24924223, 29078259], [29078260, 33232302]]
SRR24882417 file size 8026752
SRR24882417 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882417 SRR24882417_1.fastq SRR24882417_2.fastq
Input file:	SRR24882417_1.fastq
Paired file:	SRR24882417_2.fastq
trimmed:	SRR24882417-trimmed-pair1.fastq, SRR24882417-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 02:17:54 2026 >> started

Fri Jul  3 02:18:17 2026 >> done (23.061s)
33232302 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
33232302 (100.00%) read pairs available; of these:
  674474 ( 2.03%) trimmed read pairs available after processing
32557828 (97.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       3	  0.00%
 66	      22	  0.00%
 67	     491	  0.00%
 68	    3705	  0.01%
 69	    4524	  0.01%
 70	    5236	  0.02%
 71	    6131	  0.02%
 72	    7002	  0.02%
 73	    7279	  0.02%
 74	    7887	  0.02%
 75	    8847	  0.03%
 76	    9622	  0.03%
 77	   10982	  0.03%
 78	   11354	  0.03%
 79	   12095	  0.04%
 80	   13025	  0.04%
 81	   13788	  0.04%
 82	   14710	  0.04%
 83	   16087	  0.05%
 84	   17246	  0.05%
 85	   18214	  0.05%
 86	   18832	  0.06%
 87	   20594	  0.06%
 88	   22497	  0.07%
 89	   22893	  0.07%
 90	   25046	  0.08%
 91	   27471	  0.08%
 92	   28553	  0.09%
 93	   30391	  0.09%
 94	   32432	  0.10%
 95	   34090	  0.10%
 96	   35006	  0.11%
 97	   38955	  0.12%
 98	   39957	  0.12%
 99	   44501	  0.13%
100	   65006	  0.20%
101	32557828	 97.97%
33232302 reads passed initial QC


criterion=sequence-density
sequence-density=0.44
sequence-density-rank=1
fanout-score=2.97
fanout-score-rank=25
prefix-density=0.52
prefix-fanout=2.5
sequence=GATTCTGCCCAA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=26
fanout-score=25.07
fanout-score-rank=1
prefix-density=0.10
prefix-fanout=6.6
sequence=GCATCTTCTCGATCGTTTCTTCCTTGTATTTGCCCTTCTCCTTTCCTACTTGTCGGGACTTGGCTTTCCTCTCCAGGATCTTCTTGCGGTCCTTGTCCAGCTTTAGCCTGGTGATAACGACCTTGCTGGGGTGGATGCCCACATGGACGGTTGTGCCATTAGCCTTCTCTCGCTGGACTCGTTCGATGTAGATGACGTACTTCTTCCTGTACACTTGGACCACCTTGCCAATCTGCTGGCCTTTGTAGTGTCCGCGAACAACCTGAACTTCGTCATCCTTCCGAATGGGCATAGACCGAACGTTATACTTCTGTCTCAGCTCTTTGGAAAGAGGGGAAGACATGATCTTCCTCCGAATG


criterion=sequence-density
sequence-density=0.18
sequence-density-rank=1
fanout-score=8.23
fanout-score-rank=13
prefix-density=0.56
prefix-fanout=2.6
sequence=TGGGCAGAATCCCTTCTGCTGTAGGCTACCAGCCCACCCTAGCCACCGACATGGGCACAATGCAGGAAAGGATCACCACCACCAAG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=24
fanout-score=71.90
fanout-score-rank=1
prefix-density=0.28
prefix-fanout=7.7
sequence=GAAGAAGAGAATGCGCAGGCTGAAGCGCAAGAGAAGAAAGATGAGGCAGAGGTCCAAGTAAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAAGTGAGGGATGCTGAGGGCCGGGACAAGCTATCGGACTGTGTGCTGCCATCGGTAATGAGTCTCAGTAGACCTGGAACGTCACCTCGCCGCGATCGCCTGGAGAAATGACCGCCTTTCTTACAACCAAAACAGTCCCTCTGCCCTGGACCCCCGGCACTCTGGACT
SRR24882417 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 02:18:32
                             Started mapping on |	Jul 03 02:18:32
                                    Finished on |	Jul 03 02:19:50
       Mapping speed, Million of reads per hour |	1533.80

                          Number of input reads |	33232302
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27704987
                        Uniquely mapped reads % |	83.37%
                          Average mapped length |	200.25
                       Number of splices: Total |	13063821
            Number of splices: Annotated (sjdb) |	12957844
                       Number of splices: GT/AG |	12932823
                       Number of splices: GC/AG |	101446
                       Number of splices: AT/AC |	9453
               Number of splices: Non-canonical |	20099
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.04%
                        Deletion average length |	4.30
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.73
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5520506
             % of reads mapped to multiple loci |	16.61%
        Number of reads mapped to too many loci |	2566
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6809	6809	6809
N_multimapping	5520506	5520506	5520506
N_noFeature	3276368	23843859	4540848
N_ambiguous	2901893	1770677	284913
UnstrandedReadsAssigned:21526726 PositiveStrandReadsAssigned:2090451 NegativeStrandReadsAssigned:22879226
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882417 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882417-trimmed-pair1.fastq
                             SRR24882417-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 33,232,302 reads, 25,108,973 reads pseudoaligned
[quant] estimated average fragment length: 194.094
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,393 rounds

 109283 SRR24882417.ke.tsv
  52636 SRR24882417.se.tsv
 161919 total
==> SRR24882417.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR24882417.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	20
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	1
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882417 completed mapping pipeline successfully
