Starting /dee2/code/volunteer_pipeline.sh SRR24882413
    current disk space = 438558722048
    free memory = 324217876 
SRR24882413 SRAfilesize
0abb7eac16dd599d5e49852feb6592cb  SRR24882413.sra
SRR24882413.sra file validated
SRR24882413 is paired end
SRR24882413 is conventional basespace
SRR24882413 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882413_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.54525	37.0	37.0	37.0	37.0	37.0
2	36.53875	37.0	37.0	37.0	37.0	37.0
3	36.51	37.0	37.0	37.0	37.0	37.0
4	36.6405	37.0	37.0	37.0	37.0	37.0
5	36.6215	37.0	37.0	37.0	37.0	37.0
6	36.5905	37.0	37.0	37.0	37.0	37.0
7	36.625	37.0	37.0	37.0	37.0	37.0
8	36.59	37.0	37.0	37.0	37.0	37.0
9	36.568	37.0	37.0	37.0	37.0	37.0
10-11	36.6605	37.0	37.0	37.0	37.0	37.0
12-13	36.581500000000005	37.0	37.0	37.0	37.0	37.0
14-15	36.68075	37.0	37.0	37.0	37.0	37.0
16-17	36.602000000000004	37.0	37.0	37.0	37.0	37.0
18-19	36.65325	37.0	37.0	37.0	37.0	37.0
20-21	36.56625	37.0	37.0	37.0	37.0	37.0
22-23	36.629000000000005	37.0	37.0	37.0	37.0	37.0
24-25	36.587	37.0	37.0	37.0	37.0	37.0
26-27	36.55475	37.0	37.0	37.0	37.0	37.0
28-29	36.5135	37.0	37.0	37.0	37.0	37.0
30-31	36.54975	37.0	37.0	37.0	37.0	37.0
32-33	36.50875	37.0	37.0	37.0	37.0	37.0
34-35	36.47475	37.0	37.0	37.0	37.0	37.0
36-37	36.46225	37.0	37.0	37.0	37.0	37.0
38-39	36.43275	37.0	37.0	37.0	37.0	37.0
40-41	36.473	37.0	37.0	37.0	37.0	37.0
42-43	36.4645	37.0	37.0	37.0	37.0	37.0
44-45	36.43925	37.0	37.0	37.0	37.0	37.0
46-47	36.422	37.0	37.0	37.0	37.0	37.0
48-49	36.31375	37.0	37.0	37.0	37.0	37.0
50-51	36.3855	37.0	37.0	37.0	37.0	37.0
52-53	36.3595	37.0	37.0	37.0	37.0	37.0
54-55	36.29475	37.0	37.0	37.0	37.0	37.0
56-57	36.316	37.0	37.0	37.0	37.0	37.0
58-59	36.374	37.0	37.0	37.0	37.0	37.0
60-61	36.29075	37.0	37.0	37.0	37.0	37.0
62-63	36.35325	37.0	37.0	37.0	37.0	37.0
64-65	36.339	37.0	37.0	37.0	37.0	37.0
66-67	36.3825	37.0	37.0	37.0	37.0	37.0
68-69	36.307249999999996	37.0	37.0	37.0	37.0	37.0
70-71	36.314750000000004	37.0	37.0	37.0	37.0	37.0
72-73	36.346000000000004	37.0	37.0	37.0	37.0	37.0
74-75	36.2765	37.0	37.0	37.0	37.0	37.0
76-77	36.351749999999996	37.0	37.0	37.0	37.0	37.0
78-79	36.22625	37.0	37.0	37.0	37.0	37.0
80-81	36.181250000000006	37.0	37.0	37.0	37.0	37.0
82-83	36.2205	37.0	37.0	37.0	37.0	37.0
84-85	36.213	37.0	37.0	37.0	37.0	37.0
86-87	36.161249999999995	37.0	37.0	37.0	37.0	37.0
88-89	36.207	37.0	37.0	37.0	37.0	37.0
90-91	36.189499999999995	37.0	37.0	37.0	37.0	37.0
92-93	36.133750000000006	37.0	37.0	37.0	37.0	37.0
94-95	36.188	37.0	37.0	37.0	37.0	37.0
96-97	36.17175	37.0	37.0	37.0	37.0	37.0
98-99	36.181749999999994	37.0	37.0	37.0	37.0	37.0
100-101	35.99725	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	1.0
25	1.0
26	3.0
27	3.0
28	8.0
29	23.0
30	28.0
31	28.0
32	39.0
33	53.0
34	81.0
35	214.0
36	2665.0
37	852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.50937734433609	8.552138034508626	11.02775693923481	42.91072768192048
2	17.30432608152038	11.45286321580395	40.385096274068516	30.857714428607153
3	18.409204602301152	15.08254127063532	30.665332666333168	35.842921460730366
4	24.474999999999998	23.95	23.825	27.750000000000004
5	24.375	26.174999999999997	28.849999999999998	20.599999999999998
6	22.6	27.375	29.849999999999998	20.175
7	17.775	21.224999999999998	46.0	15.0
8	20.0	20.674999999999997	35.449999999999996	23.875
9	19.925	22.425	36.625	21.025
10-11	21.975	28.925	27.5875	21.512500000000003
12-13	24.087500000000002	22.900000000000002	30.362499999999997	22.650000000000002
14-15	20.45	27.6125	30.075000000000003	21.8625
16-17	21.0625	25.362499999999997	28.549999999999997	25.025
18-19	23.0	26.787499999999998	27.8625	22.35
20-21	21.2625	26.2125	28.275	24.25
22-23	23.1125	25.3125	27.675	23.9
24-25	21.9375	25.7375	28.249999999999996	24.075
26-27	21.837500000000002	26.2625	29.5	22.400000000000002
28-29	21.2875	24.9	31.3125	22.5
30-31	21.1625	24.375	29.812499999999996	24.65
32-33	21.175	25.687500000000004	30.6375	22.5
34-35	21.762500000000003	25.087500000000002	28.9	24.25
36-37	23.6375	25.05	29.849999999999998	21.462500000000002
38-39	21.6625	26.200000000000003	30.7875	21.349999999999998
40-41	22.075	25.7875	30.275000000000002	21.8625
42-43	22.0	27.625	28.287499999999998	22.0875
44-45	20.325	28.712500000000002	28.599999999999998	22.3625
46-47	21.512500000000003	27.950000000000003	26.5	24.0375
48-49	21.525	25.624999999999996	29.6875	23.1625
50-51	22.75	24.4125	29.25	23.5875
52-53	22.400000000000002	23.3	30.65	23.65
54-55	22.6	25.2375	29.262500000000003	22.900000000000002
56-57	20.200000000000003	27.0875	29.799999999999997	22.912499999999998
58-59	21.875	25.825	28.712500000000002	23.5875
60-61	21.762500000000003	26.387500000000003	28.1125	23.7375
62-63	23.3625	25.5625	28.65	22.425
64-65	23.1875	23.95	29.599999999999998	23.2625
66-67	23.9125	23.9	29.8875	22.3
68-69	22.412499999999998	24.9875	28.6625	23.9375
70-71	22.5625	27.487499999999997	26.987499999999997	22.9625
72-73	19.8625	28.6125	27.950000000000003	23.575
74-75	20.925	26.787499999999998	30.2	22.0875
76-77	24.212500000000002	26.787499999999998	26.887499999999996	22.112499999999997
78-79	23.5125	26.2625	28.462500000000002	21.762500000000003
80-81	24.1625	25.5	28.4375	21.9
82-83	22.175	27.1625	28.0875	22.575
84-85	21.975	26.825	27.0125	24.1875
86-87	24.3125	26.1125	27.987499999999997	21.587500000000002
88-89	23.7	24.75	29.95	21.6
90-91	22.1875	25.5625	29.075	23.175
92-93	21.987499999999997	25.7	29.849999999999998	22.4625
94-95	21.325	26.637499999999996	28.425	23.6125
96-97	21.6875	26.387500000000003	26.4125	25.5125
98-99	22.275	26.200000000000003	28.5625	22.9625
100-101	21.825	27.3	27.962500000000002	22.912499999999998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.5
20	2.0
21	2.0
22	5.5
23	8.5
24	7.5
25	8.5
26	10.0
27	9.0
28	13.0
29	15.0
30	36.5
31	57.0
32	80.0
33	99.5
34	91.0
35	108.0
36	99.0
37	78.0
38	68.0
39	64.5
40	85.5
41	130.0
42	203.0
43	175.0
44	112.5
45	148.0
46	227.0
47	263.5
48	216.5
49	182.0
50	186.5
51	166.0
52	144.0
53	122.0
54	104.0
55	106.5
56	104.0
57	84.0
58	93.0
59	112.0
60	73.5
61	33.0
62	19.0
63	11.0
64	5.5
65	6.0
66	3.5
67	2.0
68	2.5
69	1.5
70	1.0
71	0.0
72	0.0
73	0.0
74	1.5
75	1.5
76	0.5
77	1.5
78	1.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.025
3	0.05
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.293390394674276	32.225
2	21.30290061816453	22.400000000000002
3	8.32144555397052	13.125
4	3.518782691393248	7.3999999999999995
5	1.474084640989063	3.875
6	1.0461245839277222	3.3000000000000003
7	0.6657156443176415	2.45
8	0.5706134094151213	2.4
9	0.3804089396100808	1.7999999999999998
>10	1.4265335235378032	11.025
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	29	0.7250000000000001	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	23	0.575	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	21	0.525	No Hit
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	20	0.5	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	19	0.475	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	19	0.475	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	18	0.44999999999999996	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	17	0.42500000000000004	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	17	0.42500000000000004	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	16	0.4	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	16	0.4	No Hit
CCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAGTTGAGTTCT	16	0.4	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	15	0.375	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	14	0.35000000000000003	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	13	0.325	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	13	0.325	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	12	0.3	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	12	0.3	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	12	0.3	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	12	0.3	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	11	0.27499999999999997	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	11	0.27499999999999997	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	11	0.27499999999999997	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	11	0.27499999999999997	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	11	0.27499999999999997	No Hit
CTCGGTTAGCTTTGGTCCGTTGGTGTCTGGGGACTCTTTGGTTAGATTTC	11	0.27499999999999997	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	11	0.27499999999999997	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	10	0.25	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	10	0.25	No Hit
GTCCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATA	10	0.25	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	9	0.22499999999999998	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	9	0.22499999999999998	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	9	0.22499999999999998	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	9	0.22499999999999998	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	9	0.22499999999999998	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	9	0.22499999999999998	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	9	0.22499999999999998	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	9	0.22499999999999998	No Hit
AAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGA	8	0.2	No Hit
CTCCTCTTCAGTGACCTGTGACTGCTTGACGGCGGCAATGGCATTACTGT	8	0.2	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	8	0.2	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	8	0.2	No Hit
GTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCGGAAGGTCTTGTTTGGG	8	0.2	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	8	0.2	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	8	0.2	No Hit
CTCTAGAACTTGCATCACGTGCTTCATAGTTTTTTCTTTTACATTCCAGA	8	0.2	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	8	0.2	No Hit
TCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCCAC	8	0.2	No Hit
CCAGTGTGGTGCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAGC	8	0.2	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	8	0.2	No Hit
GATCTTTTGACTATACTGCCTGGCCATCATAAAGACCCTGCTCTTAGTCT	7	0.17500000000000002	No Hit
GGCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTA	7	0.17500000000000002	No Hit
CTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGG	7	0.17500000000000002	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	7	0.17500000000000002	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	7	0.17500000000000002	No Hit
GTCCCTCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGA	7	0.17500000000000002	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	7	0.17500000000000002	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	7	0.17500000000000002	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	7	0.17500000000000002	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	7	0.17500000000000002	No Hit
GTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTG	7	0.17500000000000002	No Hit
CCCATTTTCTGCATAACTTCTTTATGAGACAGTTTGACTTTCTTCCCAGC	7	0.17500000000000002	No Hit
CCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGTTGGAGAACGCAAAT	7	0.17500000000000002	No Hit
CTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAGAGATGAGTGCGT	7	0.17500000000000002	No Hit
CGTAATGTTGGGACATTCTGAATGTCTCAAAATGGAGTTTTTATTTCTGA	6	0.15	No Hit
GGCCTGTCTCAGGATTGCTCCAGCATTGCATCACAAGATTTCAAAATTCA	6	0.15	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	6	0.15	No Hit
GCTCTGGTTGAGGCACACGTGGCGTGCGGCCTGGTTGGCCCTACAGAAGC	6	0.15	No Hit
GTGGTTTTAAAGGCTGGGATTCTTGTGTGAGCTGTTTTCTTGCCCAGTCC	6	0.15	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	6	0.15	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	6	0.15	No Hit
CCTGTGTAGCCAGGGCTGCTCTGGACATCAGGATCTGCCTGCTTCAGCTT	6	0.15	No Hit
TTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAA	6	0.15	No Hit
CTCGTGTCGATGGCTGCGGCCAGCAAGTTATTAGCGGCAGTGATGGCATG	6	0.15	No Hit
CCCAGGTGATACTCATCTGCGGTTGCAAATGCTGTGCACTGCATCAGGTC	6	0.15	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	6	0.15	No Hit
CCTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAGAGATGAGTGCG	6	0.15	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	6	0.15	No Hit
AATGTGGGATTCCTGGTCGTTAGCTCATCGCTTCGTGTGTAACTGAAAGT	6	0.15	No Hit
GGGCTTCTCAAAGCTTCGCTATGGCTGCACACCCATTATATACTGCATAA	6	0.15	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	6	0.15	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	6	0.15	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	6	0.15	No Hit
GGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTT	6	0.15	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	6	0.15	No Hit
CTGTGTTATTGTTGGCCTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGT	5	0.125	No Hit
CTCCCGATGCAGCCTCAGGATTGTCGGGGTGCTCTGCAGGGTTGAAATCA	5	0.125	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	5	0.125	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	5	0.125	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	5	0.125	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	5	0.125	No Hit
GCACGATACTAATAATAGTTGACTCAATGGAGAAGGGAGGGGGATGAGTG	5	0.125	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	5	0.125	No Hit
CATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAA	5	0.125	No Hit
TCTCTCTTGCTGCACTCTCCTTGCCGGCAGCATCAGGACCAGCATCTGGC	5	0.125	No Hit
CGCCTCTGGAATGGACTGAATAGACTCAATAAATTTGTCCAGGGTTGCTT	5	0.125	No Hit
CTCCTTTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGGCTAATACTCT	5	0.125	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	5	0.125	No Hit
GCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTTT	5	0.125	No Hit
GTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGAT	5	0.125	No Hit
GCTTAATCTGACACTAAGGAGACTTCTTTCCAACCTGGACTGGATTAGAA	5	0.125	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	5	0.125	No Hit
GGTGCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAGCTCAAACA	5	0.125	No Hit
CGGGAATATCCTTTCTCTGTACTTCCCTGTCCAATGGTTATTTTTCTCAG	5	0.125	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	5	0.125	No Hit
GTCTGGGTTGCAGGCACCTGGGAGTCCTCTTTCACTTTGTCTGCAACGGC	5	0.125	No Hit
CAACAATTTGTTATTACATTTTATAAATGTCCTCCTACCCTTTGACCCAC	5	0.125	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	5	0.125	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	5	0.125	No Hit
GCTGCCGGGTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCGGAAGGTCT	5	0.125	No Hit
GTGTCCCCTGCGCACTGACAGCCCCTTGACTTAGAGGGTAGAGTAAGAGT	5	0.125	No Hit
GCCATAGAGACTAAAAAGGAAAAAAAAATCAGGAGGGAAAAGAAGTCGAA	5	0.125	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	5	0.125	No Hit
CCAGTGCAGAGATGAGTGCGTTTCCCTGTGTAGCCAGGGCTGCTCTGGAC	5	0.125	No Hit
CCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATACC	5	0.125	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0625	0.0	0.0	0.0	0.0
70-71	0.125	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.16249999999999998	0.0	0.0	0.0	0.0
76-77	0.36250000000000004	0.0	0.0	0.0	0.0
78-79	0.5125	0.0	0.0	0.0	0.0
80-81	0.6125	0.0	0.0	0.0	0.0
82-83	0.7125	0.0	0.0	0.0	0.0
84-85	0.8875	0.0	0.0	0.0	0.0
86-87	1.1875	0.0	0.0	0.0	0.0
88-89	1.5375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882413 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882413_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.394	37.0	37.0	37.0	37.0	37.0
2	36.3185	37.0	37.0	37.0	37.0	37.0
3	36.4655	37.0	37.0	37.0	37.0	37.0
4	36.414	37.0	37.0	37.0	37.0	37.0
5	36.417	37.0	37.0	37.0	37.0	37.0
6	36.4445	37.0	37.0	37.0	37.0	37.0
7	36.523	37.0	37.0	37.0	37.0	37.0
8	36.456	37.0	37.0	37.0	37.0	37.0
9	36.5165	37.0	37.0	37.0	37.0	37.0
10-11	36.42175	37.0	37.0	37.0	37.0	37.0
12-13	36.5045	37.0	37.0	37.0	37.0	37.0
14-15	36.463499999999996	37.0	37.0	37.0	37.0	37.0
16-17	36.396249999999995	37.0	37.0	37.0	37.0	37.0
18-19	36.415	37.0	37.0	37.0	37.0	37.0
20-21	36.386750000000006	37.0	37.0	37.0	37.0	37.0
22-23	36.39	37.0	37.0	37.0	37.0	37.0
24-25	36.462	37.0	37.0	37.0	37.0	37.0
26-27	36.43	37.0	37.0	37.0	37.0	37.0
28-29	36.386250000000004	37.0	37.0	37.0	37.0	37.0
30-31	36.3285	37.0	37.0	37.0	37.0	37.0
32-33	36.3335	37.0	37.0	37.0	37.0	37.0
34-35	36.289249999999996	37.0	37.0	37.0	37.0	37.0
36-37	36.397	37.0	37.0	37.0	37.0	37.0
38-39	36.257000000000005	37.0	37.0	37.0	37.0	37.0
40-41	36.28575	37.0	37.0	37.0	37.0	37.0
42-43	36.2525	37.0	37.0	37.0	37.0	37.0
44-45	36.23825	37.0	37.0	37.0	37.0	37.0
46-47	36.22575	37.0	37.0	37.0	37.0	37.0
48-49	36.1815	37.0	37.0	37.0	37.0	37.0
50-51	36.252250000000004	37.0	37.0	37.0	37.0	37.0
52-53	36.091499999999996	37.0	37.0	37.0	37.0	37.0
54-55	36.253	37.0	37.0	37.0	37.0	37.0
56-57	36.1215	37.0	37.0	37.0	37.0	37.0
58-59	36.13175	37.0	37.0	37.0	37.0	37.0
60-61	36.1575	37.0	37.0	37.0	37.0	37.0
62-63	36.13225	37.0	37.0	37.0	37.0	37.0
64-65	36.12475	37.0	37.0	37.0	37.0	37.0
66-67	36.125249999999994	37.0	37.0	37.0	37.0	37.0
68-69	36.161500000000004	37.0	37.0	37.0	37.0	37.0
70-71	36.05775	37.0	37.0	37.0	37.0	37.0
72-73	36.058	37.0	37.0	37.0	37.0	37.0
74-75	36.0205	37.0	37.0	37.0	37.0	37.0
76-77	36.02175	37.0	37.0	37.0	37.0	37.0
78-79	35.952749999999995	37.0	37.0	37.0	37.0	37.0
80-81	35.946	37.0	37.0	37.0	37.0	37.0
82-83	35.86425	37.0	37.0	37.0	37.0	37.0
84-85	35.85325	37.0	37.0	37.0	37.0	37.0
86-87	35.825	37.0	37.0	37.0	37.0	37.0
88-89	35.910250000000005	37.0	37.0	37.0	37.0	37.0
90-91	35.903999999999996	37.0	37.0	37.0	37.0	37.0
92-93	35.8465	37.0	37.0	37.0	37.0	37.0
94-95	35.757999999999996	37.0	37.0	37.0	37.0	37.0
96-97	35.749750000000006	37.0	37.0	37.0	37.0	37.0
98-99	35.885999999999996	37.0	37.0	37.0	37.0	37.0
100-101	35.548249999999996	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	2.0
25	2.0
26	2.0
27	10.0
28	15.0
29	13.0
30	23.0
31	25.0
32	33.0
33	74.0
34	153.0
35	445.0
36	2791.0
37	410.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.81481481481482	19.66966966966967	10.81081081081081	29.704704704704703
2	22.15	30.2	32.275	15.375
3	20.925	29.15	29.2	20.724999999999998
4	23.875	34.65	21.7	19.775000000000002
5	25.75	33.875	23.1	17.275
6	22.975	35.25	21.4	20.375
7	21.325	21.675	36.625	20.375
8	21.425	23.925	29.975	24.675
9	23.400000000000002	24.349999999999998	28.775000000000002	23.474999999999998
10-11	23.3875	34.1875	20.7	21.725
12-13	24.2625	25.6	26.7625	23.375
14-15	22.175	27.825	27.425	22.575
16-17	24.7875	28.925	26.85	19.4375
18-19	21.7875	29.925	26.0625	22.225
20-21	23.0	29.862499999999997	26.5	20.6375
22-23	23.200000000000003	29.8375	25.3125	21.65
24-25	24.1125	29.325000000000003	25.0625	21.5
26-27	23.125	29.1375	25.687500000000004	22.05
28-29	25.35	27.537499999999998	25.525	21.587500000000002
30-31	23.974999999999998	26.900000000000002	27.9375	21.1875
32-33	23.625	27.55	27.737499999999997	21.087500000000002
34-35	23.0875	29.225	25.4375	22.25
36-37	25.0	28.212500000000002	26.387500000000003	20.4
38-39	22.7625	29.225	26.3625	21.65
40-41	22.8375	28.3875	26.424999999999997	22.35
42-43	23.7875	29.1125	26.2875	20.8125
44-45	23.1875	28.487499999999997	28.025	20.3
46-47	24.7375	27.3125	25.124999999999996	22.825
48-49	24.1125	29.462500000000002	25.362499999999997	21.0625
50-51	24.025	29.6375	25.624999999999996	20.7125
52-53	23.7125	28.725	26.237500000000004	21.325
54-55	23.625	29.6875	24.7875	21.9
56-57	23.474999999999998	29.95	24.85	21.725
58-59	22.95	29.375	25.924999999999997	21.75
60-61	21.85	28.475	26.5	23.175
62-63	22.8875	29.175	25.775	22.162499999999998
64-65	23.525	28.712500000000002	26.900000000000002	20.8625
66-67	22.6125	28.475	26.325	22.5875
68-69	22.975	28.6875	26.6625	21.675
70-71	22.95	29.9875	25.95	21.1125
72-73	23.549999999999997	29.037499999999998	25.887500000000003	21.525
74-75	24.0375	27.725	26.8	21.4375
76-77	23.375	29.45	26.075	21.099999999999998
78-79	24.0125	29.262500000000003	26.025	20.7
80-81	22.650000000000002	28.6375	26.375	22.3375
82-83	22.3	30.0	26.575	21.125
84-85	22.5875	28.4125	26.174999999999997	22.825
86-87	23.1375	29.5	25.837500000000002	21.525
88-89	23.925	29.925	26.5125	19.6375
90-91	24.375	27.725	26.025	21.875
92-93	23.6875	30.0	25.825	20.4875
94-95	23.8375	29.9875	25.124999999999996	21.05
96-97	24.2875	30.525000000000002	24.4125	20.775
98-99	23.9875	27.500000000000004	26.775	21.7375
100-101	23.7125	29.3875	25.8125	21.087500000000002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.5
17	1.5
18	0.0
19	0.0
20	0.5
21	1.0
22	0.5
23	2.5
24	3.5
25	2.5
26	6.5
27	8.0
28	18.5
29	29.0
30	46.0
31	93.5
32	127.0
33	110.5
34	87.0
35	78.0
36	72.0
37	69.5
38	77.5
39	87.0
40	106.0
41	139.0
42	190.0
43	203.0
44	179.5
45	196.0
46	197.5
47	204.5
48	207.5
49	170.5
50	151.0
51	147.0
52	128.0
53	110.5
54	114.0
55	125.5
56	118.5
57	92.0
58	79.0
59	67.5
60	43.5
61	25.0
62	16.5
63	14.5
64	9.0
65	5.0
66	4.0
67	2.0
68	2.0
69	2.0
70	0.5
71	1.5
72	2.0
73	3.0
74	5.5
75	4.5
76	1.5
77	2.5
78	3.0
79	1.5
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.8342425514058	38.625
2	20.814099874108265	24.8
3	7.133864876206462	12.75
4	3.2312211498111623	7.7
5	1.3008812421317666	3.875
6	1.5107007973143098	5.4
7	0.3357112882920688	1.4000000000000001
8	0.20981955518254303	1.0
9	0.1258917331095258	0.675
>10	0.5035669324381032	3.775
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	18	0.44999999999999996	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	16	0.4	No Hit
GGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGG	15	0.375	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	13	0.325	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	13	0.325	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	13	0.325	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	11	0.27499999999999997	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	11	0.27499999999999997	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	11	0.27499999999999997	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	10	0.25	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	10	0.25	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	10	0.25	No Hit
GTGGCTCTCGTTCACTGGGAGAACGAAGAACTCAACTACATAAAGACAGA	9	0.22499999999999998	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	9	0.22499999999999998	No Hit
GCGACCTTCAAGTCTCCCCCCAAGATGGAACACTCAGTGCCGTAGCCCAG	9	0.22499999999999998	No Hit
GGAAGATACAAGTCTTTTTAAAGTTTACTGATGCTTAACTCTGTGGGCTC	8	0.2	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	8	0.2	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	8	0.2	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	8	0.2	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	8	0.2	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	7	0.17500000000000002	No Hit
CCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTAC	7	0.17500000000000002	No Hit
GGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACAC	7	0.17500000000000002	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	7	0.17500000000000002	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	7	0.17500000000000002	No Hit
CAGGAAGTCTGACCCACAGACCCCGGACCCTGACTGTTCGGATAGCATCT	7	0.17500000000000002	No Hit
CCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAAT	7	0.17500000000000002	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	7	0.17500000000000002	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	6	0.15	No Hit
AAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTG	6	0.15	No Hit
GAAGAACTCAACTACATAAAGACAGAGGGACAATCAAAACTGCACAGAGG	6	0.15	No Hit
ATCACCTGGGAAGTCTGTCTCAAGAGCTGGTCTCCTGTGGCTATGTGGAA	6	0.15	No Hit
CAGACCTAATGCGGAATCACCTCAATGAGAAGCGAGCACTCCGCTTGGAG	6	0.15	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	6	0.15	No Hit
CATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAA	6	0.15	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
GGGAGACCTGACAGAATCCTAAGACGTGAGTTGCTCATTGTAAAACTAGA	6	0.15	No Hit
CTTTCCGTTAAATGCTGTCCATTGGTTGGACGGCAAAGAACACTGGCCAA	6	0.15	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	6	0.15	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	6	0.15	No Hit
GTCGGAGACCCCACCGCCCTCAGACATTGCAGACATCGACAACTTGGAGC	6	0.15	No Hit
ATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTA	6	0.15	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	6	0.15	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	6	0.15	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	6	0.15	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	6	0.15	No Hit
GTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGGAAGGA	6	0.15	No Hit
ATCAAAACTGCACAGAGGGGAAATCAAGCTAAACTCTGAGCTGGACTTGG	6	0.15	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	6	0.15	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	6	0.15	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	6	0.15	No Hit
GGGAAATCAAGCTAAACTCTGAGCTGGACTTGGATGACGCCATTCTAGAG	6	0.15	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	6	0.15	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	6	0.15	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	6	0.15	No Hit
GTTCAGGGAAGGAGCTGCTGTGTTCTGGAATGTAAAAGAAAAAACTATGA	6	0.15	No Hit
GTCAAACTGTCTCATAAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGC	6	0.15	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	6	0.15	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	6	0.15	No Hit
CCCAAGAGTACCCCAGAGTTAGCCTTCTCAAAGAGACAGGTTAGCCACAG	6	0.15	No Hit
GTGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAG	6	0.15	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	6	0.15	No Hit
GTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGAT	6	0.15	No Hit
TCTGGGGAAAGGTAACTCGGGCCCACGGAAACAGCGGTATGGTTCGTGCC	5	0.125	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	5	0.125	No Hit
CTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGA	5	0.125	No Hit
CCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCT	5	0.125	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	5	0.125	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	5	0.125	No Hit
CCATAGTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTT	5	0.125	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	5	0.125	No Hit
TGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGG	5	0.125	No Hit
GAAATAAGTCAGAGATTACAAACTCTTCTCTACGTAAAATGTGAACATGA	5	0.125	No Hit
CACATCTTCTGTTGGGGGTTCACTGGTCTGACTTATTACCAGATGTTTCT	5	0.125	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	5	0.125	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	5	0.125	No Hit
CTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGT	5	0.125	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	5	0.125	No Hit
GGAGACCCCACCGCCCTCAGACATTGCAGACATCGACAACTTGGAGCAGG	5	0.125	No Hit
GGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGG	5	0.125	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	5	0.125	No Hit
GTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAA	5	0.125	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	5	0.125	No Hit
GGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAA	5	0.125	No Hit
GGATGTTGTGCCGGAAGGACCCGTGCAGGAAGCTGAAACTGAGCATCTTG	5	0.125	No Hit
CTCAAAATATAGTAAAAAAGTTCACAAGTTTTACCCGACATTTTTGTGGG	5	0.125	No Hit
ATTTCTTTCATGTCATAAAGAAAGCCAATCTTAATAAAATACAAGCCAAG	5	0.125	No Hit
GGAGGATTAGGAGTTTGAGGCCAGCCTGGGCAAGACCTTGTCCAAAAAAT	5	0.125	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	5	0.125	No Hit
AGGAGGTAGAGGAGGACTCCGAGGTGCTGGCTACCGAGAAAGAGAAGGAT	5	0.125	No Hit
GTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATG	5	0.125	No Hit
GTTAAGGTCATGAATGAAAAACTGCAGCACTGCATGGAACTAACAGACCT	5	0.125	No Hit
TACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATG	5	0.125	No Hit
TCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0625	0.0	0.0	0.0	0.0
70-71	0.125	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.16249999999999998	0.0	0.0	0.0	0.0
76-77	0.36250000000000004	0.0	0.0	0.0	0.0
78-79	0.525	0.0	0.0	0.0	0.0
80-81	0.6375	0.0	0.0	0.0	0.0
82-83	0.7375	0.0	0.0	0.0	0.0
84-85	0.8999999999999999	0.0	0.0	0.0	0.0
86-87	1.1625	0.0	0.0	0.0	0.0
88-89	1.4874999999999998	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025028 spots for SRR24882413.sra
Written 2025028 spots for SRR24882413.sra
Read 2025034 spots for SRR24882413.sra
Written 2025034 spots for SRR24882413.sra
SRR ids: ['SRR24882413.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_1p9b4q6a
SRR24882413.sra spots: 32400454
blocks: [[1, 2025028], [2025029, 4050056], [4050057, 6075084], [6075085, 8100112], [8100113, 10125140], [10125141, 12150168], [12150169, 14175196], [14175197, 16200224], [16200225, 18225252], [18225253, 20250280], [20250281, 22275308], [22275309, 24300336], [24300337, 26325364], [26325365, 28350392], [28350393, 30375420], [30375421, 32400454]]
SRR24882413 file size 991312
SRR24882413 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24882413 SRR24882413_1.fastq SRR24882413_2.fastq
Input file:	SRR24882413_1.fastq
Paired file:	SRR24882413_2.fastq
trimmed:	SRR24882413-trimmed-pair1.fastq, SRR24882413-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 05:54:37 2026 >> started

Fri Jul  3 05:55:37 2026 >> done (59.838s)
32400454 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
32400454 (100.00%) read pairs available; of these:
 1007660 ( 3.11%) trimmed read pairs available after processing
31392794 (96.89%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 60	       1	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       1	  0.00%
 65	       8	  0.00%
 66	     121	  0.00%
 67	    1356	  0.00%
 68	    6403	  0.02%
 69	    7799	  0.02%
 70	    8717	  0.03%
 71	    9903	  0.03%
 72	   11243	  0.03%
 73	   11832	  0.04%
 74	   12719	  0.04%
 75	   14299	  0.04%
 76	   15339	  0.05%
 77	   16683	  0.05%
 78	   17572	  0.05%
 79	   18861	  0.06%
 80	   19696	  0.06%
 81	   21859	  0.07%
 82	   23590	  0.07%
 83	   24678	  0.08%
 84	   27200	  0.08%
 85	   28672	  0.09%
 86	   29815	  0.09%
 87	   32447	  0.10%
 88	   35002	  0.11%
 89	   36073	  0.11%
 90	   37696	  0.12%
 91	   40224	  0.12%
 92	   41699	  0.13%
 93	   45165	  0.14%
 94	   47246	  0.15%
 95	   48293	  0.15%
 96	   50627	  0.16%
 97	   55590	  0.17%
 98	   57988	  0.18%
 99	   63212	  0.20%
100	   88031	  0.27%
101	31392794	 96.89%
32400454 reads passed initial QC


criterion=sequence-density
sequence-density=2.52
sequence-density-rank=1
fanout-score=1.74
fanout-score-rank=36
prefix-density=4.37
prefix-fanout=1.0
sequence=GCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAGGGCACCGGGCAGGTCATCGAGGTGGCCTGCAGCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCATATTCAGCACCATGGCCACCAATCTTCCCCCAGGCAGCCTTGATGTTGCTTTTGTCTTCCCCAGAGAGCACCATGGTTTCTTCCTGAGTCTGTCAGAAT


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=19
fanout-score=161.30
fanout-score-rank=1
prefix-density=7.25
prefix-fanout=1.1
sequence=TTCAGCACCATCCGGACAAGCTCTTCATAGTTGATGCAACCATTGCTGTCCTCATGCCCCGCCACTAGCATCTCTACTTCTTCCTCTGTCATCTTCTCGCCCAGTGTGACTAGGACATGACGGATTTCAGCACCCATGACGGTGCCATTTCCTTCCTTGTCAAACACACGAAGGCCTTCAAC


criterion=sequence-density
sequence-density=1.36
sequence-density-rank=1
fanout-score=2.06
fanout-score-rank=28
prefix-density=1.41
prefix-fanout=2.0
sequence=AAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGAA


criterion=fanout-score
sequence-density=0.16
sequence-density-rank=7
fanout-score=57.39
fanout-score-rank=1
prefix-density=9.22
prefix-fanout=1.0
sequence=TGCCTGGGGGACGATTGGTGGCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x GCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAGGGCACCGGGCAGGTCATCGAGGTGGCCTGCAGCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCATATTCAGCACCATGGCCACCAATCTTCCCCCAGGCAGCCTTGATGTTGCTTTTGTCTTCCCCAGAGAGCACCATGGTTTCTTCCTGAGTCTGTCAGAAT -y AAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAGAA -o SRR24882413 SRR24882413_1.fastq SRR24882413_2.fastq
Input file:	SRR24882413_1.fastq
Paired file:	SRR24882413_2.fastq
trimmed:	SRR24882413-trimmed-pair1.fastq, SRR24882413-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCA
-- paired 3' end adapter sequence (-y):	AAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Fri Jul  3 05:58:12 2026 >> started

Fri Jul  3 05:58:49 2026 >> done (36.507s)
10800151 read pairs processed; of these:
      69 ( 0.00%) short read pairs filtered out after trimming by size control
    3236 ( 0.03%) empty read pairs filtered out after trimming by size control
10796846 (99.97%) read pairs available; of these:
    4177 ( 0.04%) trimmed read pairs available after processing
10792669 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       4	  0.00%
 66	      62	  0.00%
 67	     593	  0.01%
 68	    2232	  0.02%
 69	    2581	  0.02%
 70	    3032	  0.03%
 71	    3482	  0.03%
 72	    3860	  0.04%
 73	    4012	  0.04%
 74	    4307	  0.04%
 75	    4751	  0.04%
 76	    5121	  0.05%
 77	    5511	  0.05%
 78	    5815	  0.05%
 79	    6119	  0.06%
 80	    6580	  0.06%
 81	    7188	  0.07%
 82	    8034	  0.07%
 83	    8232	  0.08%
 84	    8958	  0.08%
 85	    9430	  0.09%
 86	   10371	  0.10%
 87	   10994	  0.10%
 88	   11985	  0.11%
 89	   12375	  0.11%
 90	   12902	  0.12%
 91	   13271	  0.12%
 92	   13975	  0.13%
 93	   15612	  0.14%
 94	   15780	  0.15%
 95	   16623	  0.15%
 96	   17094	  0.16%
 97	   19322	  0.18%
 98	   19539	  0.18%
 99	   22766	  0.21%
100	   27878	  0.26%
101	10456455	 96.85%


criterion=sequence-density
sequence-density=1.71
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=31
prefix-density=1.74
prefix-fanout=2.0
sequence=TGTTGATCCAATAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=106.00
fanout-score-rank=1
prefix-density=0.16
prefix-fanout=8.7
sequence=TTTTCTTCCTACTCAGGCTTTATTCAAAGACCAAGAGGTACAGGTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAG


criterion=sequence-density
sequence-density=1.34
sequence-density-rank=1
fanout-score=1.99
fanout-score-rank=30
prefix-density=1.32
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=83.70
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=6.5
sequence=CAACCTCAATCTTATTAATCTTCATTCTTCTACTATCCCCAATCCTAATTTCAATATCAAACCTAATTAAACACATCAACTTCCCACTGTACACCACCACATCAATCAAATTCTCCTTCATTATTAGCCTCTTACCCCTATTAATATTTTTCCACAATAATATAGAATATATAATTACAACCTGGCACTGAGTCACCATAAATTCAATAGAACTTAAAATAAGCTTCAAAACTGACTTTTTCTCTATCCTGTTTACATCTGTAGCCCTTTTTGTCACATGATCAATTATACAATTCTCTTCATGATATATACACTCAGACCCAAACATCAATCGATTCATTAAATATCTTACACTATTCCTGATTACCATGCTTATCCTCACCTCAGCCAACAACATATTTCAACTTTTCATTGGCTGAGAAGGGGTGGGAATTATATCTTTCCTACTAATTGGATGATGGTACGGACGAACAGACGCAAATACTGCAGCCCTACAAGCAATCCTCTATAA
SRR24882413 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 06:01:11
                             Started mapping on |	Jul 03 06:01:11
                                    Finished on |	Jul 03 06:04:42
       Mapping speed, Million of reads per hour |	552.75

                          Number of input reads |	32397149
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	25577476
                        Uniquely mapped reads % |	78.95%
                          Average mapped length |	200.59
                       Number of splices: Total |	14220082
            Number of splices: Annotated (sjdb) |	14107023
                       Number of splices: GT/AG |	14081181
                       Number of splices: GC/AG |	110554
                       Number of splices: AT/AC |	10174
               Number of splices: Non-canonical |	18173
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.31
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.45
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6805873
             % of reads mapped to multiple loci |	21.01%
        Number of reads mapped to too many loci |	7252
             % of reads mapped to too many loci |	0.02%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	13800	13800	13800
N_multimapping	6805873	6805873	6805873
N_noFeature	1545510	23703260	2268289
N_ambiguous	1534515	433449	345884
UnstrandedReadsAssigned:22497451 PositiveStrandReadsAssigned:1440767 NegativeStrandReadsAssigned:22963303
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882413 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882413-trimmed-pair1.fastq
                             SRR24882413-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 32,397,149 reads, 28,034,594 reads pseudoaligned
[quant] estimated average fragment length: 184.85
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,040 rounds

 109283 SRR24882413.ke.tsv
  52636 SRR24882413.se.tsv
 161919 total
==> SRR24882413.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7.25	0	0
ENSMUST00000196221.1	9	4.75	0	0
ENSMUST00000179664.1	11	6.75	0	0
ENSMUST00000178537.1	12	6.4	0	0
ENSMUST00000178862.1	14	7.33333	0	0
ENSMUST00000179520.1	11	6.75	0	0
ENSMUST00000179883.1	16	7.25	0	0
ENSMUST00000195858.1	10	5.75	0	0
ENSMUST00000179932.1	12	6.4	0	0

==> SRR24882413.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	24
ENSMUSG00000102851	0
ENSMUSG00000103377	2
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882413 completed mapping pipeline successfully
