Starting /dee2/code/volunteer_pipeline.sh SRR24882424
    current disk space = 1407911860
    free memory = 199355868 
SRR24882424 SRAfilesize
e4e3c5bdfde86d1bc811482be369d8a0  SRR24882424.sra
SRR24882424.sra file validated
SRR24882424 is paired end
SRR24882424 is conventional basespace
SRR24882424 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.596	37.0	37.0	37.0	37.0	37.0
2	36.434	37.0	37.0	37.0	37.0	37.0
3	36.58375	37.0	37.0	37.0	37.0	37.0
4	36.5605	37.0	37.0	37.0	37.0	37.0
5	36.5855	37.0	37.0	37.0	37.0	37.0
6	36.52	37.0	37.0	37.0	37.0	37.0
7	36.545	37.0	37.0	37.0	37.0	37.0
8	36.593	37.0	37.0	37.0	37.0	37.0
9	36.6675	37.0	37.0	37.0	37.0	37.0
10-11	36.621750000000006	37.0	37.0	37.0	37.0	37.0
12-13	36.68275	37.0	37.0	37.0	37.0	37.0
14-15	36.6155	37.0	37.0	37.0	37.0	37.0
16-17	36.6045	37.0	37.0	37.0	37.0	37.0
18-19	36.560249999999996	37.0	37.0	37.0	37.0	37.0
20-21	36.632000000000005	37.0	37.0	37.0	37.0	37.0
22-23	36.56725	37.0	37.0	37.0	37.0	37.0
24-25	36.544250000000005	37.0	37.0	37.0	37.0	37.0
26-27	36.597	37.0	37.0	37.0	37.0	37.0
28-29	36.522000000000006	37.0	37.0	37.0	37.0	37.0
30-31	36.54275	37.0	37.0	37.0	37.0	37.0
32-33	36.568375	37.0	37.0	37.0	37.0	37.0
34-35	36.54174999999999	37.0	37.0	37.0	37.0	37.0
36-37	36.53	37.0	37.0	37.0	37.0	37.0
38-39	36.506375	37.0	37.0	37.0	37.0	37.0
40-41	36.4975	37.0	37.0	37.0	37.0	37.0
42-43	36.527874999999995	37.0	37.0	37.0	37.0	37.0
44-45	36.477875	37.0	37.0	37.0	37.0	37.0
46-47	36.526624999999996	37.0	37.0	37.0	37.0	37.0
48-49	36.484625	37.0	37.0	37.0	37.0	37.0
50-51	36.482	37.0	37.0	37.0	37.0	37.0
52-53	36.425875	37.0	37.0	37.0	37.0	37.0
54-55	36.406375	37.0	37.0	37.0	37.0	37.0
56-57	36.437625	37.0	37.0	37.0	37.0	37.0
58-59	36.460125	37.0	37.0	37.0	37.0	37.0
60-61	36.457375	37.0	37.0	37.0	37.0	37.0
62-63	36.392625	37.0	37.0	37.0	37.0	37.0
64-65	36.406625000000005	37.0	37.0	37.0	37.0	37.0
66-67	36.346125	37.0	37.0	37.0	37.0	37.0
68-69	36.363125	37.0	37.0	37.0	37.0	37.0
70-71	36.308125000000004	37.0	37.0	37.0	37.0	37.0
72-73	36.326375	37.0	37.0	37.0	37.0	37.0
74-75	36.292875	37.0	37.0	37.0	37.0	37.0
76-77	36.383875	37.0	37.0	37.0	37.0	37.0
78-79	36.292125	37.0	37.0	37.0	37.0	37.0
80-81	36.306375	37.0	37.0	37.0	37.0	37.0
82-83	36.293	37.0	37.0	37.0	37.0	37.0
84-85	36.27975	37.0	37.0	37.0	37.0	37.0
86-87	36.334625	37.0	37.0	37.0	37.0	37.0
88-89	36.27475	37.0	37.0	37.0	37.0	37.0
90-91	36.23125	37.0	37.0	37.0	37.0	37.0
92-93	36.233375	37.0	37.0	37.0	37.0	37.0
94-95	36.18475	37.0	37.0	37.0	37.0	37.0
96-97	36.192499999999995	37.0	37.0	37.0	37.0	37.0
98-99	36.200500000000005	37.0	37.0	37.0	37.0	37.0
100-101	36.07899999999999	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	0.0
25	1.0
26	2.0
27	3.0
28	12.0
29	13.0
30	14.0
31	22.0
32	35.0
33	43.0
34	79.0
35	253.0
36	2698.0
37	824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.525	10.625	11.799999999999999	42.05
2	18.575	15.15	38.05	28.225
3	19.454863715928983	18.754688672168044	30.75768942235559	31.032758189547387
4	24.975	26.875	24.775	23.375
5	23.799999999999997	31.900000000000002	25.025	19.275000000000002
6	19.275000000000002	32.625	28.775000000000002	19.325
7	16.775000000000002	22.95	43.2	17.075000000000003
8	20.25	21.55	35.099999999999994	23.1
9	20.200000000000003	22.625	35.75	21.425
10-11	21.3125	31.5625	25.15	21.975
12-13	22.6375	24.099999999999998	28.1375	25.124999999999996
14-15	20.4625	27.737499999999997	30.525000000000002	21.275
16-17	20.525	28.212500000000002	27.1	24.1625
18-19	22.9375	28.599999999999998	27.0	21.462500000000002
20-21	22.537499999999998	27.3625	28.512500000000003	21.587500000000002
22-23	22.475	27.487499999999997	26.724999999999998	23.3125
24-25	23.625	26.5875	25.5625	24.224999999999998
26-27	20.4375	29.349999999999998	29.1125	21.099999999999998
28-29	21.875	28.4125	28.4375	21.275
30-31	22.25	27.500000000000004	28.050000000000004	22.2
32-33	22.877859732466558	26.02825353169146	28.92861607700963	22.165270658832352
34-35	20.0	27.737499999999997	28.487499999999997	23.775
36-37	23.8125	28.199999999999996	27.474999999999998	20.5125
38-39	21.315164395549445	30.028753594199276	28.96612076509564	19.689961245155644
40-41	20.0625	30.125	28.425	21.3875
42-43	23.090386298287285	30.041255156894614	26.153269158644832	20.715089386173272
44-45	20.127515939492437	29.716214526815854	27.640955119389925	22.515314414301788
46-47	22.665333166645834	29.216152019002372	25.028128516064506	23.090386298287285
48-49	21.540192524065507	30.05375671958995	25.128141017627204	23.27790973871734
50-51	21.349999999999998	26.650000000000002	29.0875	22.912499999999998
52-53	22.165270658832352	24.428053506688336	30.12876609576197	23.27790973871734
54-55	20.015001875234404	26.828353544193025	28.766095761970245	24.390548818602326
56-57	21.215151893986747	28.34104263032879	28.82860357544693	21.61520190023753
58-59	21.140142517814727	26.95336917114639	28.766095761970245	23.140392549068633
60-61	20.877609701212652	29.341167645955746	26.803350418802353	22.977872234029252
62-63	22.515314414301788	28.153519189898734	27.090886360795096	22.240280035004375
64-65	21.9777472184023	26.253281660207527	28.178522315289413	23.590448806100763
66-67	23.56544568071009	27.91598949868734	27.11588948618577	21.402675334416802
68-69	22.00275034379297	28.653581697712216	26.615826978372297	22.727840980122515
70-71	22.540317539692463	28.91611451431429	26.190773846730842	22.352794099262407
72-73	22.477809726215778	30.466308288536066	26.765845730716343	20.290036254531817
74-75	21.765220652581576	27.640955119389925	28.27853481685211	22.315289411176398
76-77	23.1278909863733	28.753594199274907	26.178272284035504	21.94024253031629
78-79	23.92799099887486	25.95324415551944	27.353419177397175	22.765345668208525
80-81	24.028003500437556	26.115764470558823	28.34104263032879	21.515189398674835
82-83	22.5	27.900000000000002	28.237499999999997	21.3625
84-85	22.775000000000002	28.15	25.775	23.3
86-87	23.06538317289661	28.003500437554695	27.428428553569194	21.502687835979497
88-89	24.4	26.2875	29.2875	20.025000000000002
90-91	21.8625	28.95	26.937499999999996	22.25
92-93	22.615326915864483	28.01600200025003	26.828353544193025	22.540317539692463
94-95	21.712500000000002	29.7	26.387500000000003	22.2
96-97	21.637500000000003	28.4125	24.2	25.75
98-99	22.475	27.6	27.35	22.575
100-101	23.8125	26.4125	26.700000000000003	23.075000000000003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	3.0
20	4.0
21	2.5
22	1.5
23	1.0
24	1.5
25	12.0
26	19.5
27	14.0
28	15.0
29	18.5
30	34.0
31	60.5
32	103.0
33	115.5
34	108.5
35	130.5
36	114.0
37	99.0
38	85.0
39	82.5
40	98.0
41	137.5
42	210.5
43	185.5
44	150.0
45	167.5
46	214.0
47	246.0
48	195.0
49	162.0
50	162.0
51	138.5
52	120.0
53	105.5
54	98.5
55	92.0
56	66.5
57	55.5
58	59.0
59	78.0
60	85.0
61	52.5
62	34.0
63	20.0
64	3.5
65	4.0
66	2.5
67	1.5
68	1.0
69	3.0
70	4.5
71	2.5
72	0.5
73	1.0
74	5.5
75	4.5
76	1.0
77	2.0
78	1.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0125
34-35	0.0
36-37	0.0
38-39	0.0125
40-41	0.0
42-43	0.0125
44-45	0.0125
46-47	0.0125
48-49	0.0125
50-51	0.0
52-53	0.0125
54-55	0.0125
56-57	0.0125
58-59	0.0125
60-61	0.0125
62-63	0.0125
64-65	0.0125
66-67	0.0125
68-69	0.0125
70-71	0.0125
72-73	0.0125
74-75	0.0125
76-77	0.0125
78-79	0.0125
80-81	0.0125
82-83	0.0
84-85	0.0
86-87	0.0125
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.824999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.64919129949804	23.599999999999998
2	23.36865588399331	20.95
3	9.927495817066369	13.350000000000001
4	5.186837702175126	9.3
5	2.5655326268823204	5.75
6	2.0635805911879532	5.55
7	0.7808142777467931	2.45
8	0.6692693809258227	2.4
9	0.5577244841048522	2.25
>10	2.230897936419409	14.399999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	28	0.7000000000000001	No Hit
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	28	0.7000000000000001	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	23	0.575	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	22	0.5499999999999999	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	21	0.525	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	20	0.5	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	20	0.5	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	20	0.5	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	20	0.5	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	19	0.475	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	16	0.4	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	16	0.4	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	14	0.35000000000000003	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	14	0.35000000000000003	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	14	0.35000000000000003	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	13	0.325	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	13	0.325	No Hit
GTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGAT	13	0.325	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	13	0.325	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	12	0.3	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	12	0.3	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	12	0.3	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	12	0.3	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	11	0.27499999999999997	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	11	0.27499999999999997	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	11	0.27499999999999997	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	11	0.27499999999999997	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	11	0.27499999999999997	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	11	0.27499999999999997	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	11	0.27499999999999997	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	11	0.27499999999999997	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	11	0.27499999999999997	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	11	0.27499999999999997	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	11	0.27499999999999997	No Hit
GCCAGTGTGGTGCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAG	10	0.25	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	10	0.25	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	10	0.25	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	10	0.25	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	10	0.25	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	10	0.25	No Hit
CTCCAGATTGCTGCTCTTCATGTGCTCAACTATCCAATTAAAGTCATGCT	9	0.22499999999999998	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	9	0.22499999999999998	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	9	0.22499999999999998	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	9	0.22499999999999998	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	9	0.22499999999999998	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	9	0.22499999999999998	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	9	0.22499999999999998	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	9	0.22499999999999998	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	9	0.22499999999999998	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	9	0.22499999999999998	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	8	0.2	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	8	0.2	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	8	0.2	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	8	0.2	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	8	0.2	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	8	0.2	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	8	0.2	No Hit
TTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTT	8	0.2	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	8	0.2	No Hit
CCTGCCAGTGGTCAGCCAGTGGGGATCAGAGGCCGTGAGCTGCTCCGCCT	8	0.2	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	8	0.2	No Hit
CTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAGAGATGAGTGCGT	8	0.2	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	7	0.17500000000000002	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	7	0.17500000000000002	No Hit
GTTTGTCTGTCTGCAGTTCATTCCGTAATTTAGAAAGAAGAGAGATGGCT	7	0.17500000000000002	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	7	0.17500000000000002	No Hit
GCTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAA	7	0.17500000000000002	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	7	0.17500000000000002	No Hit
CTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGC	7	0.17500000000000002	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	7	0.17500000000000002	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	7	0.17500000000000002	No Hit
CCTCACTTCTGGCTCCGTTGCATCCTGGGAGACCACTTTCTTATTGGCCC	7	0.17500000000000002	No Hit
GAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATT	7	0.17500000000000002	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	7	0.17500000000000002	No Hit
AGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTT	7	0.17500000000000002	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	7	0.17500000000000002	No Hit
ACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGA	6	0.15	No Hit
CGCCAATACTAAATCCGTAATCAGTTCAAAGCCAGAATTATCCAGAACAA	6	0.15	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	6	0.15	No Hit
CGTCGAAGTCATGGATTGGCGGACTCTGCATGATGGCTTCATGAATTCTC	6	0.15	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	6	0.15	No Hit
GGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAAC	6	0.15	No Hit
GCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATT	6	0.15	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	6	0.15	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	6	0.15	No Hit
CAGGAACCGAAACACAGCATCTTCTTGTTCTTCTTGGAAAAAGGCCAGCC	6	0.15	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	6	0.15	No Hit
CACTTCATAGGGCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCT	6	0.15	No Hit
CAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGA	6	0.15	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	6	0.15	No Hit
GACCTATAACCTATACCTAGATTCACTTTTCCATTCAGCTCTAGTGAGAA	6	0.15	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	6	0.15	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	6	0.15	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	6	0.15	No Hit
CCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGG	6	0.15	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	6	0.15	No Hit
GGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
CCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATC	6	0.15	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	6	0.15	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	6	0.15	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	6	0.15	No Hit
GAGACAGACTTCCCAGGTGATACTCATCTGCGGTTGCAAATGCTGTGCAC	6	0.15	No Hit
TGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACAT	6	0.15	No Hit
GGATGGGGGCTTCTGGGAGGCAGGGAACCAGAGAAGTGGATAACATTTGA	6	0.15	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	6	0.15	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	6	0.15	No Hit
GGGATATTCAGTTTCTAACATACACGTAGGGTATAGCTATGCACTACCCT	6	0.15	No Hit
AGGGCATCTCCTTCCTCGGTTAGCTTTGGTCCGTTGGTGTCTGGGGACTC	6	0.15	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	6	0.15	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	6	0.15	No Hit
TAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAAC	6	0.15	No Hit
GCTCAACTATCCAATTAAAGTCATGCTCAGTAACGTCAGACACAAACCAT	6	0.15	No Hit
CAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGT	6	0.15	No Hit
TAGGTGTCAATGCTCTGGTTGAGGCACACGTGGCGTGCGGCCTGGTTGGC	5	0.125	No Hit
TCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGA	5	0.125	No Hit
GGTAATTCTGTGTGTTCAAGCATCATGCACACGCCTCCCGGGAGAGCTCG	5	0.125	No Hit
ATACAACTTTAAAAAACCACAATGAGGCAATAAGTACATGACCTGGCAAA	5	0.125	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	5	0.125	No Hit
TCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCT	5	0.125	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	5	0.125	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	5	0.125	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	5	0.125	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	5	0.125	No Hit
GAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCG	5	0.125	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	5	0.125	No Hit
CTTGAATAAAATAAGATGTGCCTTTTGCAGTTCAGCGTATAAGTCAGGGG	5	0.125	No Hit
CACCCATTTTCTGCATAACTTCTTTATGAGACAGTTTGACTTTCTTCCCA	5	0.125	No Hit
TATTGTCATTGAGTTTTTTGTTTGTACTCTTGCTTTATATATCTATATAT	5	0.125	No Hit
CCCACATGAAAGACATCGTGATGACAAACTATACTGGAGACTAAGTGCTT	5	0.125	No Hit
CCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGA	5	0.125	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	5	0.125	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	5	0.125	No Hit
CTTAACTACTGCGGCTCTCTGAAGCATAGGGAATTCATCTCGAAGGACAG	5	0.125	No Hit
AGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATA	5	0.125	No Hit
TACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAA	5	0.125	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	5	0.125	No Hit
CCAGCTTTTAATGCCTCTGGAATGGACTGAATAGACTCAATAAATTTGTC	5	0.125	No Hit
GCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGG	5	0.125	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	5	0.125	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	5	0.125	No Hit
CCCAACTAAAACTCCATTTGCACACCTTCTCTTCAAGAGTCTGTTTCCAT	5	0.125	No Hit
CTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTT	5	0.125	No Hit
GGCCGTGGGCCTCCTCCTTAGCAGACTTTGGAAGGAATAAAAGTCAGAGA	5	0.125	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	5	0.125	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	5	0.125	No Hit
CTGGAATGGACTGAATAGACTCAATAAATTTGTCCAGGGTTGCTTCCCAA	5	0.125	No Hit
GATTATTTCCGGTGTTTCCTCCTGCTCGTCCTTTGTGATATCTTCAACAA	5	0.125	No Hit
TATAAGTCAGGGGCGACCTGGGGCATCACACAATATGGATGAGGCAGAGT	5	0.125	No Hit
CCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCGTATTTCA	5	0.125	No Hit
CTTCCAAAGGCAACTCCACCTTCTCATAGTCGGCTGACAACCTGGCCTGG	5	0.125	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	5	0.125	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	5	0.125	No Hit
GCTCCTTACGGTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGT	5	0.125	No Hit
CAATGAAATAATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACAT	5	0.125	No Hit
TTTCAAATCTCATTTTAAAACAAGTGTGTCAGGAACCGAAACACAGCATC	5	0.125	No Hit
CACTCTTGGGCTCCTTCATTCTTGTGTTCAATGGTTTCAAAGGTTAAAGA	5	0.125	No Hit
CCAAACCAATGCAGCGGGGCAAACCATTCCAAGGAAGCCTCGTCTACTGT	5	0.125	No Hit
GTCAGGTTTTATCCAAAAATGAAGATTGCCGTCACATTATTACTCTTTTG	5	0.125	No Hit
CCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGGTGATCAAGAAATCTGAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0875	0.0	0.0	0.0	0.0
74-75	0.1375	0.0	0.0	0.0	0.0
76-77	0.1875	0.0	0.0	0.0	0.0
78-79	0.30000000000000004	0.0	0.0	0.0	0.0
80-81	0.375	0.0	0.0	0.0	0.0
82-83	0.55	0.0	0.0	0.0	0.0
84-85	0.7	0.0	0.0	0.0	0.0
86-87	0.85	0.0	0.0	0.0	0.0
88-89	0.9625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882424 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882424_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.49	37.0	37.0	37.0	37.0	37.0
2	36.4085	37.0	37.0	37.0	37.0	37.0
3	36.395	37.0	37.0	37.0	37.0	37.0
4	36.4535	37.0	37.0	37.0	37.0	37.0
5	36.4665	37.0	37.0	37.0	37.0	37.0
6	36.2915	37.0	37.0	37.0	37.0	37.0
7	36.4705	37.0	37.0	37.0	37.0	37.0
8	36.4075	37.0	37.0	37.0	37.0	37.0
9	36.4345	37.0	37.0	37.0	37.0	37.0
10-11	36.42275	37.0	37.0	37.0	37.0	37.0
12-13	36.485749999999996	37.0	37.0	37.0	37.0	37.0
14-15	36.377250000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.4095	37.0	37.0	37.0	37.0	37.0
18-19	36.37225	37.0	37.0	37.0	37.0	37.0
20-21	36.3925	37.0	37.0	37.0	37.0	37.0
22-23	36.400000000000006	37.0	37.0	37.0	37.0	37.0
24-25	36.36025	37.0	37.0	37.0	37.0	37.0
26-27	36.3865	37.0	37.0	37.0	37.0	37.0
28-29	36.266999999999996	37.0	37.0	37.0	37.0	37.0
30-31	36.28975	37.0	37.0	37.0	37.0	37.0
32-33	36.257	37.0	37.0	37.0	37.0	37.0
34-35	36.29275	37.0	37.0	37.0	37.0	37.0
36-37	36.2675	37.0	37.0	37.0	37.0	37.0
38-39	36.31125	37.0	37.0	37.0	37.0	37.0
40-41	36.262	37.0	37.0	37.0	37.0	37.0
42-43	36.185	37.0	37.0	37.0	37.0	37.0
44-45	36.10625	37.0	37.0	37.0	37.0	37.0
46-47	36.16275	37.0	37.0	37.0	37.0	37.0
48-49	36.13975	37.0	37.0	37.0	37.0	37.0
50-51	36.098	37.0	37.0	37.0	37.0	37.0
52-53	36.10825	37.0	37.0	37.0	37.0	37.0
54-55	36.134	37.0	37.0	37.0	37.0	37.0
56-57	36.15075	37.0	37.0	37.0	37.0	37.0
58-59	36.0455	37.0	37.0	37.0	37.0	37.0
60-61	36.045	37.0	37.0	37.0	37.0	37.0
62-63	36.050250000000005	37.0	37.0	37.0	37.0	37.0
64-65	36.11925	37.0	37.0	37.0	37.0	37.0
66-67	35.989999999999995	37.0	37.0	37.0	37.0	37.0
68-69	35.971999999999994	37.0	37.0	37.0	37.0	37.0
70-71	35.94075	37.0	37.0	37.0	37.0	37.0
72-73	35.94675	37.0	37.0	37.0	37.0	37.0
74-75	35.964749999999995	37.0	37.0	37.0	37.0	37.0
76-77	35.97925	37.0	37.0	37.0	37.0	37.0
78-79	35.88575	37.0	37.0	37.0	37.0	37.0
80-81	35.85675	37.0	37.0	37.0	37.0	37.0
82-83	35.942750000000004	37.0	37.0	37.0	37.0	37.0
84-85	35.90625	37.0	37.0	37.0	37.0	37.0
86-87	35.908249999999995	37.0	37.0	37.0	37.0	37.0
88-89	35.846999999999994	37.0	37.0	37.0	37.0	37.0
90-91	35.818	37.0	37.0	37.0	37.0	37.0
92-93	35.7525	37.0	37.0	37.0	37.0	37.0
94-95	35.76975	37.0	37.0	37.0	37.0	37.0
96-97	35.804249999999996	37.0	37.0	37.0	37.0	37.0
98-99	35.76675	37.0	37.0	37.0	37.0	37.0
100-101	35.48325	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	1.0
24	3.0
25	3.0
26	6.0
27	7.0
28	10.0
29	15.0
30	14.0
31	30.0
32	38.0
33	85.0
34	161.0
35	535.0
36	2726.0
37	365.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.11311311311311	19.76976976976977	13.038038038038039	29.07907907907908
2	21.85	25.900000000000002	35.15	17.1
3	21.05	26.650000000000002	31.025000000000002	21.275
4	24.825	31.7	24.175	19.3
5	25.6	32.75	24.55	17.1
6	21.6	35.925000000000004	24.275	18.2
7	21.725	19.6	38.800000000000004	19.875
8	21.525	22.2	30.599999999999998	25.674999999999997
9	21.349999999999998	24.325	31.075000000000003	23.25
10-11	24.85	29.7	23.549999999999997	21.9
12-13	23.05	24.6875	27.6125	24.65
14-15	23.95	26.0625	28.762500000000003	21.224999999999998
16-17	24.075	27.400000000000002	29.15	19.375
18-19	23.150000000000002	28.287499999999998	28.050000000000004	20.5125
20-21	23.9	27.925	27.6625	20.5125
22-23	23.825	28.475	26.687499999999996	21.0125
24-25	23.7	28.812500000000004	26.9625	20.525
26-27	23.775	27.1625	27.787499999999998	21.275
28-29	22.725	27.6875	28.249999999999996	21.337500000000002
30-31	24.637500000000003	26.650000000000002	28.462500000000002	20.25
32-33	24.0	26.087500000000002	29.9	20.0125
34-35	24.3125	28.299999999999997	26.9625	20.424999999999997
36-37	23.5625	28.349999999999998	26.875	21.212500000000002
38-39	23.0	27.787499999999998	27.150000000000002	22.0625
40-41	22.8625	27.287499999999998	28.875	20.974999999999998
42-43	22.662499999999998	27.237499999999997	28.237499999999997	21.8625
44-45	23.8125	26.974999999999998	28.787499999999998	20.424999999999997
46-47	24.325	26.75	26.025	22.900000000000002
48-49	23.6125	26.737499999999997	28.599999999999998	21.05
50-51	22.5625	27.500000000000004	27.287499999999998	22.650000000000002
52-53	22.975	27.237499999999997	29.175	20.6125
54-55	22.975	27.9375	26.187500000000004	22.900000000000002
56-57	23.2625	27.8875	27.1125	21.7375
58-59	21.775	27.8125	28.175	22.237499999999997
60-61	20.5625	28.8875	27.6125	22.9375
62-63	23.5375	26.950000000000003	27.212500000000002	22.3
64-65	23.9	27.6625	28.799999999999997	19.6375
66-67	23.1875	26.474999999999998	27.8625	22.475
68-69	22.375	28.075	27.712500000000002	21.837500000000002
70-71	22.7375	28.962500000000002	28.025	20.275000000000002
72-73	23.2875	28.1875	26.1625	22.3625
74-75	25.575	27.35	28.050000000000004	19.025
76-77	23.25	29.262500000000003	25.337500000000002	22.15
78-79	23.150000000000002	27.787499999999998	28.6375	20.424999999999997
80-81	22.650000000000002	27.750000000000004	28.349999999999998	21.25
82-83	22.3625	27.187499999999996	29.325000000000003	21.125
84-85	22.4375	26.937499999999996	28.462500000000002	22.162499999999998
86-87	21.987499999999997	28.4375	26.6	22.975
88-89	23.075000000000003	28.4375	27.400000000000002	21.087500000000002
90-91	23.400000000000002	27.4125	26.087500000000002	23.1
92-93	22.95	28.7375	27.1375	21.175
94-95	23.775	28.375	25.887500000000003	21.9625
96-97	23.6375	28.4	26.3	21.6625
98-99	22.325	28.0625	27.712500000000002	21.9
100-101	24.45	27.224999999999998	26.9625	21.3625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.5
20	1.0
21	1.0
22	2.0
23	2.0
24	2.0
25	2.5
26	3.0
27	5.5
28	8.0
29	19.5
30	54.5
31	103.0
32	135.0
33	116.0
34	90.0
35	98.0
36	101.0
37	108.5
38	107.0
39	105.0
40	122.0
41	147.0
42	199.0
43	197.5
44	184.0
45	198.0
46	209.0
47	238.5
48	185.0
49	117.0
50	129.5
51	126.5
52	104.0
53	94.5
54	90.5
55	97.0
56	84.0
57	58.5
58	66.0
59	63.0
60	32.0
61	15.5
62	17.0
63	15.0
64	9.0
65	7.5
66	3.0
67	4.0
68	5.5
69	6.0
70	4.5
71	7.0
72	14.0
73	11.5
74	17.0
75	17.5
76	10.5
77	14.0
78	8.5
79	2.0
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.3081305023304	26.700000000000003
2	22.32004142931124	21.55
3	9.735888140859657	14.099999999999998
4	4.764370792335577	9.2
5	2.4339720352149143	5.875
6	1.3464526152252718	3.9
7	1.1393060590367685	3.85
8	0.7250129466597618	2.8000000000000003
9	0.6214396685655101	2.7
>10	1.605385810460901	9.325
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	21	0.525	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	16	0.4	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	16	0.4	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	15	0.375	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	15	0.375	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	14	0.35000000000000003	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	14	0.35000000000000003	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	14	0.35000000000000003	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	13	0.325	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	12	0.3	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	12	0.3	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	12	0.3	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	12	0.3	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	12	0.3	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	11	0.27499999999999997	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	11	0.27499999999999997	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	11	0.27499999999999997	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	11	0.27499999999999997	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	11	0.27499999999999997	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	10	0.25	No Hit
CAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAG	10	0.25	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	10	0.25	No Hit
CCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTAC	10	0.25	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	10	0.25	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	10	0.25	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	10	0.25	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	10	0.25	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	10	0.25	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	10	0.25	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	10	0.25	No Hit
ATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATG	10	0.25	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	9	0.22499999999999998	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	9	0.22499999999999998	No Hit
GGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGG	9	0.22499999999999998	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	9	0.22499999999999998	No Hit
GCAGAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGAT	9	0.22499999999999998	No Hit
ATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAA	9	0.22499999999999998	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	9	0.22499999999999998	No Hit
ATCAAAACTGCACAGAGGGGAAATCAAGCTAAACTCTGAGCTGGACTTGG	9	0.22499999999999998	No Hit
GAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGC	9	0.22499999999999998	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	9	0.22499999999999998	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	9	0.22499999999999998	No Hit
CTGAGATTCATTTCCATGGGAAAAGCATCCCATGGTTTGTGTCTGACGTT	9	0.22499999999999998	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	8	0.2	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	8	0.2	No Hit
TGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACAC	8	0.2	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	8	0.2	No Hit
GTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGA	8	0.2	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	8	0.2	No Hit
GTGTGATGCCCCAGGTCGCCCCTGACTTATACGCTGAACTGCAAAAGGCA	8	0.2	No Hit
TTTTAATCTTTGTCCTCTAGGTGTCTGATTTCAGAATGTATCCTTTGAGA	8	0.2	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	8	0.2	No Hit
GTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGT	8	0.2	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	8	0.2	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	8	0.2	No Hit
AGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATAC	8	0.2	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	8	0.2	No Hit
GTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACA	7	0.17500000000000002	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	7	0.17500000000000002	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	7	0.17500000000000002	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	7	0.17500000000000002	No Hit
GTCCTTTGCATATCTTACGATTAAGGACAGAACACCGCAGATCTTAACCA	7	0.17500000000000002	No Hit
CCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTA	7	0.17500000000000002	No Hit
GGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTT	7	0.17500000000000002	No Hit
CTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGA	7	0.17500000000000002	No Hit
CCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCT	7	0.17500000000000002	No Hit
CTCATTCTTTCCAAGTATAACAGCTGACAGTTTGAATAAGCCAGAGATCC	7	0.17500000000000002	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	7	0.17500000000000002	No Hit
AGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAG	7	0.17500000000000002	No Hit
GGCGGCCGGTGGCGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTC	7	0.17500000000000002	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	7	0.17500000000000002	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	7	0.17500000000000002	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	7	0.17500000000000002	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	7	0.17500000000000002	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	7	0.17500000000000002	No Hit
GAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTT	7	0.17500000000000002	No Hit
CTCTCTTCTTTCTAAATTACGGAATGAACTGCAGACAGACAAACCAATCA	7	0.17500000000000002	No Hit
CCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCAT	7	0.17500000000000002	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	7	0.17500000000000002	No Hit
GGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCA	6	0.15	No Hit
CCTGACTTCTACTGGGACAGAGCAAACCTGGAAGAGCTTTATGACAAGAC	6	0.15	No Hit
TAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTT	6	0.15	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	6	0.15	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	6	0.15	No Hit
GGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAG	6	0.15	No Hit
GCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAA	6	0.15	No Hit
AACACTGGCCAAGCATGCACCCCTTTGGGACTCACAGGAGCATGACCCAC	6	0.15	No Hit
AGTGTTGAGAAGCTGCCTCTTCAGTGCAAAGATGAAAAGGAACATGCTGC	6	0.15	No Hit
GTGAATTTTGCTTTAAAAGTTAATTTAAATGTTAGTTAATCCTGTTTGTG	6	0.15	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	6	0.15	No Hit
ATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCT	6	0.15	No Hit
GTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCT	6	0.15	No Hit
GTATGCACTATCTGCATGTACATTAGGGGTGTGTGCGCTGGAGTGTGCAT	6	0.15	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	6	0.15	No Hit
GGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGC	6	0.15	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	6	0.15	No Hit
GTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATT	6	0.15	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	6	0.15	No Hit
GTAGAAACGCCCGTAGTTAGAGTAGACATTGTTCTGGATAATTCTGGCTT	6	0.15	No Hit
CAGAGGGACAATCAAAACTGCACAGAGGGGAAATCAAGCTAAACTCTGAG	6	0.15	No Hit
AGCTTGAGTGAGCTTTTGTGAACTGCTGGTACTTGAAAGGCTTAACAGCT	6	0.15	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	6	0.15	No Hit
CCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGC	6	0.15	No Hit
GTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTA	6	0.15	No Hit
CAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAG	6	0.15	No Hit
CATTGTTCTGGATAATTCTGGCTTTGAACTGATTACGGATTTAGTATTGG	5	0.125	No Hit
GTGACATCTACTAAAGCATCAAAAATAACAAATCAGGACACAAAATCTTT	5	0.125	No Hit
GGCGGAGAGCGACACGCCGAGCGAGCTGGAGCTCAGTGGCCATGGGCCCG	5	0.125	No Hit
TGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGT	5	0.125	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	5	0.125	No Hit
GCCAGATGGACAATATGCTGGTGCAGGCCAACAAGCCCTACAAGTGCAAC	5	0.125	No Hit
CCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTT	5	0.125	No Hit
GGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAACCAAAGGAAGATGATC	5	0.125	No Hit
TCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTT	5	0.125	No Hit
GTACCAGAGACCTCGCAGCTGTCCCAGCTGACTTCAAACCTAGCCCAGGT	5	0.125	No Hit
GCCTGATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATC	5	0.125	No Hit
ATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTCATGTACTTATTG	5	0.125	No Hit
GTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACA	5	0.125	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	5	0.125	No Hit
GATGATCGTCATCCTCATCACCATTGAGGTAATGTTTGAGCTGGGGAGAG	5	0.125	No Hit
GCCACCGTATGCGGCTGTTGGGGCTGCTGTGGTAGCGGCGCGGGGCGCTC	5	0.125	No Hit
CCTGTCTCTCTGCAGCAATGCGGCCGACCTGGGCAAGGATGCTCTGGAAG	5	0.125	No Hit
ATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCC	5	0.125	No Hit
GACAAGCCTGCCTAGAGATGCAGCAAATATTTTGGTGATGGGTGTGGAAA	5	0.125	No Hit
GTCCAGGCTAGCCTCAGCTTCATAACAAACTCTGTCTCAGCCACTGGAGT	5	0.125	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	5	0.125	No Hit
CACCTGGGAAGTCTGTCTCAAGAGCTGGTCTCCTGTGGCTATGTGGAAGT	5	0.125	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	5	0.125	No Hit
GAACATTCTGAGTCTCCCTTCAGCCTCAGTGCATGGATTTAGGCCTGGCT	5	0.125	No Hit
GGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTG	5	0.125	No Hit
GAACAAGGCTGCTGAAACTTCCTCTCTGAGCAGAACCTTTCCATACAAGA	5	0.125	No Hit
ATCCTTTGAGATTATGTCCGGTTAAGGAAAATCACTAGTTAAGAGAACTA	5	0.125	No Hit
GGGAAGTGAAGAGGCGCAAGTATGAGTGCACGATCTGCGGCCGCAAGTTC	5	0.125	No Hit
TTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATA	5	0.125	No Hit
GATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGG	5	0.125	No Hit
CACGAAGAAAACAGCAAATTGCTAATTCACCAAGAGCCATGCAAGGATTG	5	0.125	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	5	0.125	No Hit
GCGGCGGTGCGCGTGTCTTCTTCCAGAGTCCCCGCGGTGGAACCGGCGGC	5	0.125	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	5	0.125	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	5	0.125	No Hit
ATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTCC	5	0.125	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	5	0.125	No Hit
CTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTA	5	0.125	No Hit
CCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTAC	5	0.125	No Hit
GTAGTTCTCAGAAGGCCAATATAATAAATTGTTTGCAAGACCTAAAGCCA	5	0.125	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	5	0.125	No Hit
CCCTCATCCAGGAAGCCAGCCTTGCCAGCCAAGGCAGCTTTTCCCATCCC	5	0.125	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	5	0.125	No Hit
CACAAATAGAGCAGACACAAAACCAAGTAATTTTAAGACTGTTATATTTA	5	0.125	No Hit
AATAAACCTGAGCTCAGATTTCTTGATCACCCCTGACTTCTACTGGGACA	5	0.125	No Hit
ATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGA	5	0.125	No Hit
GTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0875	0.0	0.0	0.0	0.0
74-75	0.1375	0.0	0.0	0.0	0.0
76-77	0.1875	0.0	0.0	0.0	0.0
78-79	0.30000000000000004	0.0	0.0	0.0	0.0
80-81	0.375	0.0	0.0	0.0	0.0
82-83	0.55	0.0	0.0	0.0	0.0
84-85	0.7	0.0	0.0	0.0	0.0
86-87	0.85	0.0	0.0	0.0	0.0
88-89	0.925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275056 spots for SRR24882424.sra
Written 4275056 spots for SRR24882424.sra
Read 4275062 spots for SRR24882424.sra
Written 4275062 spots for SRR24882424.sra
SRR ids: ['SRR24882424.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_3vdkfipv
SRR24882424.sra spots: 34200454
blocks: [[1, 4275056], [4275057, 8550112], [8550113, 12825168], [12825169, 17100224], [17100225, 21375280], [21375281, 25650336], [25650337, 29925392], [29925393, 34200454]]
SRR24882424 file size 8261228
SRR24882424 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882424 SRR24882424_1.fastq SRR24882424_2.fastq
Input file:	SRR24882424_1.fastq
Paired file:	SRR24882424_2.fastq
trimmed:	SRR24882424-trimmed-pair1.fastq, SRR24882424-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 02:39:45 2026 >> started

Fri Jul  3 02:40:13 2026 >> done (27.902s)
34200454 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
34200454 (100.00%) read pairs available; of these:
  642758 ( 1.88%) trimmed read pairs available after processing
33557696 (98.12%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       1	  0.00%
 66	      21	  0.00%
 67	     971	  0.00%
 68	    4613	  0.01%
 69	    5641	  0.02%
 70	    6616	  0.02%
 71	    7129	  0.02%
 72	    7830	  0.02%
 73	    8663	  0.03%
 74	    9036	  0.03%
 75	    9854	  0.03%
 76	   10767	  0.03%
 77	   11460	  0.03%
 78	   12150	  0.04%
 79	   13006	  0.04%
 80	   13726	  0.04%
 81	   14552	  0.04%
 82	   15491	  0.05%
 83	   16290	  0.05%
 84	   17530	  0.05%
 85	   18750	  0.05%
 86	   18734	  0.05%
 87	   20240	  0.06%
 88	   21706	  0.06%
 89	   21819	  0.06%
 90	   24104	  0.07%
 91	   25258	  0.07%
 92	   25913	  0.08%
 93	   26747	  0.08%
 94	   28684	  0.08%
 95	   29100	  0.09%
 96	   30971	  0.09%
 97	   33043	  0.10%
 98	   33763	  0.10%
 99	   36798	  0.11%
100	   61781	  0.18%
101	33557696	 98.12%
34200454 reads passed initial QC


criterion=sequence-density
sequence-density=0.91
sequence-density-rank=1
fanout-score=2.43
fanout-score-rank=33
prefix-density=0.99
prefix-fanout=2.2
sequence=AGGACCAGGTGCAGGGT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=65.39
fanout-score-rank=1
prefix-density=0.13
prefix-fanout=6.8
sequence=GATTTTCTTCTCTTGTTGGAACTTTACTTTCCTTTTCAGATTTTTCAATCTTCTTCTTCCCTGAGTCTGGAACTTCTGGTGCTCCCTTTGAAGATGGTTTAGGCTTATCTTTTTTTATAGATGGGTGGCCTTTTTCTTGGTGTTCAGCTTTTTCTGGTTTAACATGAGGAATAACTTGCTCTGGTCTGGTGATGTTCTGTTTGCGTGTTTGGCCTTTGTCAGATGATGCTAGTTTTGCTTCCTTTTTCACTTGTGGCT


criterion=sequence-density
sequence-density=0.51
sequence-density-rank=1
fanout-score=6.62
fanout-score-rank=16
prefix-density=0.79
prefix-fanout=4.3
sequence=TGGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGCCGCACCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=47.88
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=3.4
sequence=GCCGCCGCCATCGTCGGCGCGCTTCCCTGTTCACCTCTGACTCTGAGAATCCGTCGCCATCCGCCACCATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGGAACATGTCAGTCATCGCCCATGTGGACCACGGCAAGTCCACGCTGACCGACTCCCTTGTGTGCAAGGCTGGCATCATTGCCTCTGCCCGAGCTGGGGAGACGCGCTTCACTGACACTCGCAAGGATGAGCAGGAGCGCTGCATCACAATCAAATCCACCGCCATCTCCCTCTTCTACGAGCTCTCTGAGAACGACCTGAACTTCATTAAGCAGAGCAAGGATGGCTCGGGCTTCCTCATCAACCTCATCGACTCTCCAGGCCATGTGGACTTCTCTTCAGAGGTGACAGCTGCCTTGCGTGTCACCGATGGAGCTCTGGTGGTGGTG
SRR24882424 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 02:40:27
                             Started mapping on |	Jul 03 02:40:27
                                    Finished on |	Jul 03 03:02:33
       Mapping speed, Million of reads per hour |	92.85

                          Number of input reads |	34200454
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	31330495
                        Uniquely mapped reads % |	91.61%
                          Average mapped length |	200.69
                       Number of splices: Total |	24759143
            Number of splices: Annotated (sjdb) |	24568982
                       Number of splices: GT/AG |	24570526
                       Number of splices: GC/AG |	150592
                       Number of splices: AT/AC |	15675
               Number of splices: Non-canonical |	22350
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	3.00
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.63
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2862978
             % of reads mapped to multiple loci |	8.37%
        Number of reads mapped to too many loci |	2809
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6981	6981	6981
N_multimapping	2862978	2862978	2862978
N_noFeature	1739225	28488834	2358327
N_ambiguous	2529862	609958	307237
UnstrandedReadsAssigned:27061408 PositiveStrandReadsAssigned:2231703 NegativeStrandReadsAssigned:28664931
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882424 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882424-trimmed-pair1.fastq
                             SRR24882424-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 34,200,454 reads, 29,987,270 reads pseudoaligned
[quant] estimated average fragment length: 223.215
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,144 rounds

 109283 SRR24882424.ke.tsv
  52636 SRR24882424.se.tsv
 161919 total
==> SRR24882424.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.6	0	0
ENSMUST00000196221.1	9	1	0	0
ENSMUST00000179664.1	11	3	0	0
ENSMUST00000178537.1	12	2	0	0
ENSMUST00000178862.1	14	3.5	0	0
ENSMUST00000179520.1	11	3	0	0
ENSMUST00000179883.1	16	4.6	0	0
ENSMUST00000195858.1	10	2	0	0
ENSMUST00000179932.1	12	2	0	0

==> SRR24882424.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882424 completed mapping pipeline successfully
