Starting /dee2/code/volunteer_pipeline.sh SRR24882409
    current disk space = 1344209752
    free memory = 199412296 
SRR24882409 SRAfilesize
951b2347ade0cef467c01a8ff85a0513  SRR24882409.sra
SRR24882409.sra file validated
SRR24882409 is paired end
SRR24882409 is conventional basespace
SRR24882409 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.61125	37.0	37.0	37.0	37.0	37.0
2	36.40525	37.0	37.0	37.0	37.0	37.0
3	36.53525	37.0	37.0	37.0	37.0	37.0
4	36.5215	37.0	37.0	37.0	37.0	37.0
5	36.613	37.0	37.0	37.0	37.0	37.0
6	36.5765	37.0	37.0	37.0	37.0	37.0
7	36.586	37.0	37.0	37.0	37.0	37.0
8	36.4825	37.0	37.0	37.0	37.0	37.0
9	36.6135	37.0	37.0	37.0	37.0	37.0
10-11	36.5385	37.0	37.0	37.0	37.0	37.0
12-13	36.607749999999996	37.0	37.0	37.0	37.0	37.0
14-15	36.517250000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.57425	37.0	37.0	37.0	37.0	37.0
18-19	36.534	37.0	37.0	37.0	37.0	37.0
20-21	36.563	37.0	37.0	37.0	37.0	37.0
22-23	36.588	37.0	37.0	37.0	37.0	37.0
24-25	36.56625	37.0	37.0	37.0	37.0	37.0
26-27	36.50675	37.0	37.0	37.0	37.0	37.0
28-29	36.502250000000004	37.0	37.0	37.0	37.0	37.0
30-31	36.46575	37.0	37.0	37.0	37.0	37.0
32-33	36.504	37.0	37.0	37.0	37.0	37.0
34-35	36.50075	37.0	37.0	37.0	37.0	37.0
36-37	36.5445	37.0	37.0	37.0	37.0	37.0
38-39	36.457750000000004	37.0	37.0	37.0	37.0	37.0
40-41	36.48425	37.0	37.0	37.0	37.0	37.0
42-43	36.40075	37.0	37.0	37.0	37.0	37.0
44-45	36.39475	37.0	37.0	37.0	37.0	37.0
46-47	36.417500000000004	37.0	37.0	37.0	37.0	37.0
48-49	36.391000000000005	37.0	37.0	37.0	37.0	37.0
50-51	36.339	37.0	37.0	37.0	37.0	37.0
52-53	36.285	37.0	37.0	37.0	37.0	37.0
54-55	36.42375	37.0	37.0	37.0	37.0	37.0
56-57	36.35425	37.0	37.0	37.0	37.0	37.0
58-59	36.442750000000004	37.0	37.0	37.0	37.0	37.0
60-61	36.38175	37.0	37.0	37.0	37.0	37.0
62-63	36.384	37.0	37.0	37.0	37.0	37.0
64-65	36.37975	37.0	37.0	37.0	37.0	37.0
66-67	36.25025	37.0	37.0	37.0	37.0	37.0
68-69	36.243750000000006	37.0	37.0	37.0	37.0	37.0
70-71	36.27475	37.0	37.0	37.0	37.0	37.0
72-73	36.310500000000005	37.0	37.0	37.0	37.0	37.0
74-75	36.155249999999995	37.0	37.0	37.0	37.0	37.0
76-77	36.2565	37.0	37.0	37.0	37.0	37.0
78-79	36.205749999999995	37.0	37.0	37.0	37.0	37.0
80-81	36.242000000000004	37.0	37.0	37.0	37.0	37.0
82-83	36.17975	37.0	37.0	37.0	37.0	37.0
84-85	36.202749999999995	37.0	37.0	37.0	37.0	37.0
86-87	36.19925	37.0	37.0	37.0	37.0	37.0
88-89	36.24225	37.0	37.0	37.0	37.0	37.0
90-91	36.188	37.0	37.0	37.0	37.0	37.0
92-93	36.17675	37.0	37.0	37.0	37.0	37.0
94-95	36.20525	37.0	37.0	37.0	37.0	37.0
96-97	36.027	37.0	37.0	37.0	37.0	37.0
98-99	36.05325	37.0	37.0	37.0	37.0	37.0
100-101	35.954750000000004	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	5.0
26	2.0
27	5.0
28	15.0
29	17.0
30	24.0
31	33.0
32	31.0
33	52.0
34	81.0
35	247.0
36	2611.0
37	876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.7344336084021	9.052263065766441	10.877719429857464	42.335583895974
2	19.654913728432106	12.678169542385595	35.65891472868217	32.00800200050013
3	19.479869967491872	16.25406351587897	31.557889472368096	32.70817704426106
4	25.474999999999998	24.725	24.9	24.9
5	23.825	28.125	25.074999999999996	22.975
6	21.125	32.425	27.425	19.025
7	15.625	26.224999999999998	44.175	13.975000000000001
8	21.349999999999998	24.175	32.5	21.975
9	20.175	24.25	33.95	21.625
10-11	20.275000000000002	33.9625	25.9875	19.775000000000002
12-13	21.6	27.1375	29.3375	21.925
14-15	19.8	30.175	29.0875	20.9375
16-17	19.8625	28.812500000000004	27.525	23.799999999999997
18-19	22.325	30.575000000000003	25.687500000000004	21.4125
20-21	22.825	28.475	26.35	22.35
22-23	21.9625	27.6375	26.5	23.9
24-25	22.9625	28.712500000000002	26.0	22.325
26-27	21.3875	30.675	27.275	20.6625
28-29	19.8	29.4875	28.812500000000004	21.9
30-31	21.075	28.8625	28.9125	21.15
32-33	20.962500000000002	27.55	30.5125	20.974999999999998
34-35	18.55	28.9375	29.2	23.3125
36-37	21.837500000000002	28.6875	28.762500000000003	20.7125
38-39	22.2	28.9875	30.0	18.8125
40-41	20.474999999999998	30.1875	29.4125	19.925
42-43	21.224999999999998	32.95	26.937499999999996	18.8875
44-45	19.5875	32.6875	26.0125	21.712500000000002
46-47	22.575	32.15	22.8875	22.3875
48-49	21.075	32.25	25.1875	21.4875
50-51	22.2	28.775000000000002	27.287499999999998	21.7375
52-53	22.5875	26.2875	28.5875	22.537499999999998
54-55	20.6625	27.975	28.025	23.3375
56-57	19.375	28.15	30.0	22.475
58-59	20.575	27.775	28.325	23.325000000000003
60-61	20.325	28.7	26.974999999999998	24.0
62-63	22.75	29.475	26.35	21.425
64-65	22.912499999999998	26.650000000000002	27.6375	22.8
66-67	23.200000000000003	26.924999999999997	29.049999999999997	20.825
68-69	22.15	28.299999999999997	26.924999999999997	22.625
70-71	22.900000000000002	29.25	26.5625	21.2875
72-73	20.3875	30.625000000000004	27.962500000000002	21.025
74-75	21.2	29.762499999999996	28.875	20.1625
76-77	24.05	29.2375	26.137500000000003	20.575
78-79	23.0375	27.212500000000002	28.775000000000002	20.974999999999998
80-81	25.15	26.937499999999996	27.3625	20.549999999999997
82-83	24.3125	28.799999999999997	26.8375	20.05
84-85	21.0125	29.8375	27.700000000000003	21.45
86-87	22.925	29.099999999999998	25.924999999999997	22.05
88-89	23.8875	28.262500000000003	28.287499999999998	19.5625
90-91	22.425	29.1125	27.0625	21.4
92-93	21.4	29.299999999999997	27.275	22.025
94-95	21.55	31.087500000000002	25.9625	21.4
96-97	21.4	29.4375	23.35	25.8125
98-99	21.15	29.7375	27.800000000000004	21.3125
100-101	22.25	30.325000000000003	25.974999999999998	21.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	3.0
22	6.0
23	5.5
24	4.0
25	9.5
26	16.0
27	18.0
28	16.5
29	17.0
30	43.5
31	86.0
32	128.0
33	151.0
34	149.0
35	174.5
36	161.5
37	109.5
38	73.0
39	63.0
40	99.5
41	159.0
42	211.5
43	187.5
44	136.0
45	140.0
46	232.0
47	277.0
48	185.0
49	109.5
50	110.5
51	113.0
52	87.5
53	78.0
54	73.5
55	68.0
56	67.0
57	48.5
58	48.0
59	102.0
60	95.0
61	33.0
62	19.0
63	15.0
64	7.5
65	6.0
66	5.0
67	4.0
68	6.5
69	8.5
70	7.0
71	2.5
72	2.0
73	4.5
74	4.5
75	3.5
76	2.5
77	1.0
78	2.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.025
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.97097625329816	32.675
2	14.406332453825858	13.65
3	5.013192612137203	7.124999999999999
4	2.9023746701846966	5.5
5	1.7414248021108178	4.125
6	1.5303430079155673	4.35
7	1.0554089709762533	3.5000000000000004
8	0.5277044854881267	2.0
9	0.4221635883905013	1.7999999999999998
>10	3.37730870712401	23.549999999999997
>50	0.05277044854881266	1.725
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	69	1.725	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	41	1.0250000000000001	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	35	0.8750000000000001	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	33	0.8250000000000001	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	27	0.675	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	26	0.65	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	24	0.6	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	23	0.575	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	21	0.525	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	20	0.5	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	19	0.475	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	19	0.475	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	18	0.44999999999999996	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	18	0.44999999999999996	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	17	0.42500000000000004	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	17	0.42500000000000004	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	16	0.4	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	16	0.4	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	16	0.4	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	16	0.4	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	15	0.375	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	15	0.375	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	15	0.375	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	14	0.35000000000000003	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	14	0.35000000000000003	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	14	0.35000000000000003	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	14	0.35000000000000003	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	14	0.35000000000000003	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	13	0.325	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	13	0.325	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	13	0.325	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	12	0.3	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	12	0.3	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	12	0.3	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	12	0.3	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	12	0.3	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	12	0.3	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	12	0.3	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	11	0.27499999999999997	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	11	0.27499999999999997	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	11	0.27499999999999997	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	11	0.27499999999999997	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	11	0.27499999999999997	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	11	0.27499999999999997	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	11	0.27499999999999997	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	11	0.27499999999999997	No Hit
CTCCCTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTA	11	0.27499999999999997	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	11	0.27499999999999997	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	11	0.27499999999999997	No Hit
GTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAAC	11	0.27499999999999997	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	10	0.25	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	10	0.25	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	10	0.25	No Hit
GCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTAG	10	0.25	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	10	0.25	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	10	0.25	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	10	0.25	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	10	0.25	No Hit
GCTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAA	10	0.25	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	10	0.25	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	10	0.25	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	10	0.25	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	10	0.25	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	10	0.25	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	10	0.25	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	9	0.22499999999999998	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	9	0.22499999999999998	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	9	0.22499999999999998	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	9	0.22499999999999998	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	9	0.22499999999999998	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	9	0.22499999999999998	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	9	0.22499999999999998	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	9	0.22499999999999998	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	8	0.2	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	8	0.2	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	8	0.2	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	8	0.2	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	8	0.2	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	8	0.2	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	8	0.2	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	8	0.2	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	8	0.2	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	8	0.2	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	7	0.17500000000000002	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	7	0.17500000000000002	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	7	0.17500000000000002	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	7	0.17500000000000002	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	7	0.17500000000000002	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	7	0.17500000000000002	No Hit
ACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAA	7	0.17500000000000002	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	7	0.17500000000000002	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	7	0.17500000000000002	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	7	0.17500000000000002	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	7	0.17500000000000002	No Hit
CCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCT	7	0.17500000000000002	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	7	0.17500000000000002	No Hit
CTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTT	7	0.17500000000000002	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	7	0.17500000000000002	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	7	0.17500000000000002	No Hit
GTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACC	7	0.17500000000000002	No Hit
GTCCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCC	7	0.17500000000000002	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	7	0.17500000000000002	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	7	0.17500000000000002	No Hit
AGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATC	6	0.15	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	6	0.15	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	6	0.15	No Hit
AGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCT	6	0.15	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	6	0.15	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	6	0.15	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	6	0.15	No Hit
CGTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGAT	6	0.15	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	6	0.15	No Hit
TTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACAT	6	0.15	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	6	0.15	No Hit
CCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAA	6	0.15	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	6	0.15	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	6	0.15	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	6	0.15	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	6	0.15	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	6	0.15	No Hit
TGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATT	6	0.15	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	6	0.15	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	6	0.15	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	6	0.15	No Hit
CCTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCC	6	0.15	No Hit
CTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCC	6	0.15	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	6	0.15	No Hit
GGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAA	6	0.15	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	6	0.15	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	6	0.15	No Hit
GACAGGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAG	5	0.125	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	5	0.125	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	5	0.125	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	5	0.125	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	5	0.125	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	5	0.125	No Hit
CGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCT	5	0.125	No Hit
CTGTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTA	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	5	0.125	No Hit
GGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGA	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAAC	5	0.125	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	5	0.125	No Hit
AGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	5	0.125	No Hit
CTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCTCACAT	5	0.125	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	5	0.125	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	5	0.125	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	5	0.125	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	5	0.125	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	5	0.125	No Hit
ACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAG	5	0.125	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	5	0.125	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	5	0.125	No Hit
GTCCAATGGCCTTTGCAGGAAGGTTGCTTCGGAATTTGGCACGAACCATA	5	0.125	No Hit
CCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATC	5	0.125	No Hit
CCTCAATCTACATAATGCCTTTTGGAGGCTGGTTCACAACTTGGTCAGAT	5	0.125	No Hit
ATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGC	5	0.125	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	5	0.125	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	5	0.125	No Hit
GGGCTACAGTCCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAAT	5	0.125	No Hit
TCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGGCAATAAG	5	0.125	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	5	0.125	No Hit
CTGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTA	5	0.125	No Hit
TGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.025	0.0	0.0	0.0	0.0
76-77	0.2	0.0	0.0	0.0	0.0
78-79	0.30000000000000004	0.0	0.0	0.0	0.0
80-81	0.35	0.0	0.0	0.0	0.0
82-83	0.48750000000000004	0.0	0.0	0.0	0.0
84-85	0.7	0.0	0.0	0.0	0.0
86-87	0.875	0.0	0.0	0.0	0.0
88-89	1.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882409 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882409_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2625	37.0	37.0	37.0	37.0	37.0
2	36.195	37.0	37.0	37.0	37.0	37.0
3	36.321	37.0	37.0	37.0	37.0	37.0
4	36.2735	37.0	37.0	37.0	37.0	37.0
5	36.438	37.0	37.0	37.0	37.0	37.0
6	36.378	37.0	37.0	37.0	37.0	37.0
7	36.4065	37.0	37.0	37.0	37.0	37.0
8	36.4955	37.0	37.0	37.0	37.0	37.0
9	36.3975	37.0	37.0	37.0	37.0	37.0
10-11	36.35825	37.0	37.0	37.0	37.0	37.0
12-13	36.41825	37.0	37.0	37.0	37.0	37.0
14-15	36.417	37.0	37.0	37.0	37.0	37.0
16-17	36.354749999999996	37.0	37.0	37.0	37.0	37.0
18-19	36.37	37.0	37.0	37.0	37.0	37.0
20-21	36.35875	37.0	37.0	37.0	37.0	37.0
22-23	36.3765	37.0	37.0	37.0	37.0	37.0
24-25	36.32125	37.0	37.0	37.0	37.0	37.0
26-27	36.3475	37.0	37.0	37.0	37.0	37.0
28-29	36.346000000000004	37.0	37.0	37.0	37.0	37.0
30-31	36.31225	37.0	37.0	37.0	37.0	37.0
32-33	36.32475	37.0	37.0	37.0	37.0	37.0
34-35	36.2225	37.0	37.0	37.0	37.0	37.0
36-37	36.2825	37.0	37.0	37.0	37.0	37.0
38-39	36.3155	37.0	37.0	37.0	37.0	37.0
40-41	36.226749999999996	37.0	37.0	37.0	37.0	37.0
42-43	36.164500000000004	37.0	37.0	37.0	37.0	37.0
44-45	36.1805	37.0	37.0	37.0	37.0	37.0
46-47	36.0975	37.0	37.0	37.0	37.0	37.0
48-49	35.9885	37.0	37.0	37.0	37.0	37.0
50-51	36.12825	37.0	37.0	37.0	37.0	37.0
52-53	36.10424999999999	37.0	37.0	37.0	37.0	37.0
54-55	36.1035	37.0	37.0	37.0	37.0	37.0
56-57	35.9875	37.0	37.0	37.0	37.0	37.0
58-59	36.0895	37.0	37.0	37.0	37.0	37.0
60-61	35.967	37.0	37.0	37.0	37.0	37.0
62-63	35.95325	37.0	37.0	37.0	37.0	37.0
64-65	35.9985	37.0	37.0	37.0	37.0	37.0
66-67	36.01925	37.0	37.0	37.0	37.0	37.0
68-69	35.94799999999999	37.0	37.0	37.0	37.0	37.0
70-71	35.872249999999994	37.0	37.0	37.0	37.0	37.0
72-73	35.838	37.0	37.0	37.0	37.0	37.0
74-75	35.96025	37.0	37.0	37.0	37.0	37.0
76-77	35.89875	37.0	37.0	37.0	37.0	37.0
78-79	35.93375	37.0	37.0	37.0	37.0	37.0
80-81	35.83275	37.0	37.0	37.0	37.0	37.0
82-83	35.775999999999996	37.0	37.0	37.0	37.0	37.0
84-85	35.78274999999999	37.0	37.0	37.0	37.0	37.0
86-87	35.841750000000005	37.0	37.0	37.0	37.0	37.0
88-89	35.706	37.0	37.0	37.0	37.0	37.0
90-91	35.810500000000005	37.0	37.0	37.0	37.0	37.0
92-93	35.67275	37.0	37.0	37.0	37.0	37.0
94-95	35.648250000000004	37.0	37.0	37.0	37.0	37.0
96-97	35.6755	37.0	37.0	37.0	37.0	37.0
98-99	35.68775	37.0	37.0	37.0	37.0	37.0
100-101	35.503	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	0.0
24	8.0
25	6.0
26	7.0
27	8.0
28	13.0
29	17.0
30	24.0
31	36.0
32	43.0
33	80.0
34	169.0
35	472.0
36	2755.0
37	361.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.23535302954432	20.70605908863295	13.26990485728593	25.78868302453681
2	22.325	26.025	35.225	16.425
3	18.925	27.750000000000004	32.775	20.549999999999997
4	24.349999999999998	33.525	22.775000000000002	19.35
5	24.925	32.824999999999996	25.074999999999996	17.175
6	22.7	33.625	25.025	18.65
7	20.65	22.650000000000002	38.3	18.4
8	22.125	23.625	30.55	23.7
9	22.525000000000002	23.75	31.374999999999996	22.35
10-11	24.175	31.75	23.875	20.200000000000003
12-13	24.4875	25.0125	26.625	23.875
14-15	24.05	26.3625	30.5125	19.075
16-17	23.549999999999997	27.650000000000002	30.2	18.6
18-19	23.7	27.3625	29.099999999999998	19.8375
20-21	21.45	29.3375	30.0875	19.125
22-23	21.325	29.2875	27.9375	21.45
24-25	22.1	29.75	27.6625	20.4875
26-27	22.275	27.8625	28.1375	21.725
28-29	23.075000000000003	26.487500000000004	29.349999999999998	21.087500000000002
30-31	23.0125	26.950000000000003	29.175	20.8625
32-33	23.075000000000003	25.45	30.587500000000002	20.8875
34-35	23.325000000000003	26.224999999999998	28.762500000000003	21.6875
36-37	22.0625	27.474999999999998	30.25	20.2125
38-39	22.1375	27.287499999999998	27.6625	22.912499999999998
40-41	21.0125	27.462500000000002	29.8375	21.6875
42-43	22.075	29.1125	28.1125	20.7
44-45	20.5125	28.462500000000002	30.7875	20.2375
46-47	22.162499999999998	28.4375	26.924999999999997	22.475
48-49	21.925	27.275	30.55	20.25
50-51	21.5375	26.9625	28.4	23.1
52-53	21.525	28.525	29.45	20.5
54-55	23.3875	26.674999999999997	27.9125	22.025
56-57	20.9	27.725	28.7375	22.6375
58-59	20.9125	28.225	28.725	22.1375
60-61	20.3875	29.025000000000002	28.9375	21.65
62-63	20.5625	28.8875	28.9375	21.6125
64-65	22.9375	26.787499999999998	30.062499999999996	20.2125
66-67	20.175	26.35	30.5125	22.9625
68-69	21.987499999999997	27.975	29.9625	20.075000000000003
70-71	21.425	27.1	30.825000000000003	20.65
72-73	22.475	27.0625	28.775000000000002	21.6875
74-75	23.849999999999998	25.8	29.45	20.9
76-77	21.6	26.924999999999997	28.749999999999996	22.725
78-79	22.45	26.5625	30.1375	20.849999999999998
80-81	21.4875	26.7125	29.9	21.9
82-83	21.7875	26.5625	30.3875	21.2625
84-85	21.025	26.5125	30.375000000000004	22.0875
86-87	21.4125	27.825	29.625	21.1375
88-89	21.837500000000002	27.200000000000003	29.349999999999998	21.6125
90-91	23.4625	26.375	28.212500000000002	21.95
92-93	23.2125	29.4	27.8875	19.5
94-95	22.037499999999998	30.8	26.787499999999998	20.375
96-97	22.7125	28.825	27.375	21.087500000000002
98-99	20.549999999999997	29.312500000000004	28.037499999999998	22.1
100-101	22.900000000000002	28.199999999999996	28.6875	20.2125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.5
16	1.5
17	1.0
18	0.0
19	2.0
20	2.5
21	0.5
22	1.0
23	1.0
24	3.0
25	4.5
26	4.0
27	4.5
28	6.5
29	21.0
30	55.0
31	115.5
32	176.0
33	167.5
34	124.5
35	105.0
36	108.0
37	116.0
38	102.5
39	99.5
40	130.5
41	164.0
42	198.0
43	226.5
44	244.5
45	235.5
46	192.5
47	181.0
48	166.0
49	126.5
50	110.0
51	102.5
52	82.5
53	59.0
54	69.0
55	103.0
56	73.0
57	27.0
58	46.0
59	53.5
60	31.5
61	22.0
62	18.5
63	12.5
64	8.0
65	5.0
66	4.0
67	8.0
68	9.0
69	4.5
70	3.0
71	11.0
72	10.0
73	2.5
74	6.5
75	5.0
76	7.0
77	11.5
78	5.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.47455162384877	34.8
2	14.590402326708677	15.049999999999999
3	6.204556471158507	9.6
4	3.6354823073194376	7.5
5	2.375181774115366	6.125
6	1.4541929229277752	4.5
7	0.9694619486185166	3.5000000000000004
8	0.8240426563257393	3.4000000000000004
9	0.9694619486185166	4.5
>10	1.502666020358701	11.025
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	28	0.7000000000000001	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	27	0.675	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	22	0.5499999999999999	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	21	0.525	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	18	0.44999999999999996	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	18	0.44999999999999996	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	16	0.4	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	16	0.4	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	15	0.375	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	15	0.375	No Hit
GTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATG	15	0.375	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	14	0.35000000000000003	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	14	0.35000000000000003	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	13	0.325	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	13	0.325	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	13	0.325	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	12	0.3	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	12	0.3	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	12	0.3	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	11	0.27499999999999997	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	11	0.27499999999999997	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	11	0.27499999999999997	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	11	0.27499999999999997	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	11	0.27499999999999997	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	11	0.27499999999999997	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	11	0.27499999999999997	No Hit
TGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGAC	10	0.25	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	10	0.25	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	10	0.25	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	10	0.25	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	10	0.25	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	9	0.22499999999999998	No Hit
TGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTG	9	0.22499999999999998	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	9	0.22499999999999998	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	9	0.22499999999999998	No Hit
AGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAA	9	0.22499999999999998	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	9	0.22499999999999998	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	9	0.22499999999999998	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	9	0.22499999999999998	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	9	0.22499999999999998	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	9	0.22499999999999998	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	9	0.22499999999999998	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	9	0.22499999999999998	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	9	0.22499999999999998	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	9	0.22499999999999998	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	9	0.22499999999999998	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	9	0.22499999999999998	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	9	0.22499999999999998	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	9	0.22499999999999998	No Hit
CTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGT	9	0.22499999999999998	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	9	0.22499999999999998	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	8	0.2	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	8	0.2	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	8	0.2	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	8	0.2	No Hit
GTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAG	8	0.2	No Hit
CCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTAC	8	0.2	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	8	0.2	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	8	0.2	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	8	0.2	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	8	0.2	No Hit
GTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTG	8	0.2	No Hit
AAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACT	8	0.2	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	8	0.2	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	8	0.2	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	8	0.2	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	8	0.2	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	8	0.2	No Hit
GTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACT	7	0.17500000000000002	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	7	0.17500000000000002	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	7	0.17500000000000002	No Hit
GGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGA	7	0.17500000000000002	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	7	0.17500000000000002	No Hit
GGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACA	7	0.17500000000000002	No Hit
ACCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGA	7	0.17500000000000002	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	7	0.17500000000000002	No Hit
GTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAG	7	0.17500000000000002	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	7	0.17500000000000002	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	7	0.17500000000000002	No Hit
GTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTT	7	0.17500000000000002	No Hit
CTTGTATATATCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGT	7	0.17500000000000002	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	7	0.17500000000000002	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	7	0.17500000000000002	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	7	0.17500000000000002	No Hit
AAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAA	7	0.17500000000000002	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	7	0.17500000000000002	No Hit
CTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTC	7	0.17500000000000002	No Hit
ACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTT	7	0.17500000000000002	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	6	0.15	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	6	0.15	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	6	0.15	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	6	0.15	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	6	0.15	No Hit
CTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGA	6	0.15	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	6	0.15	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	6	0.15	No Hit
ATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCC	6	0.15	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	6	0.15	No Hit
CCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTT	6	0.15	No Hit
GACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCT	6	0.15	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	6	0.15	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	6	0.15	No Hit
GAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAA	6	0.15	No Hit
AATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAAT	6	0.15	No Hit
TGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTGAT	6	0.15	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	6	0.15	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	6	0.15	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	6	0.15	No Hit
CTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTC	6	0.15	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	6	0.15	No Hit
TTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATC	6	0.15	No Hit
CAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTC	6	0.15	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	6	0.15	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	6	0.15	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	6	0.15	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	6	0.15	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	5	0.125	No Hit
AACGACGCCACCAGCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAG	5	0.125	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	5	0.125	No Hit
AGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTC	5	0.125	No Hit
ATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCT	5	0.125	No Hit
TGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGAC	5	0.125	No Hit
CCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGAT	5	0.125	No Hit
CTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGC	5	0.125	No Hit
CTTCCTTCCAGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCG	5	0.125	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	5	0.125	No Hit
TGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTT	5	0.125	No Hit
GCAGAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGAT	5	0.125	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	5	0.125	No Hit
GTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTT	5	0.125	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	5	0.125	No Hit
AGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGA	5	0.125	No Hit
ATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCT	5	0.125	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	5	0.125	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	5	0.125	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
CTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTCTC	5	0.125	No Hit
AGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTA	5	0.125	No Hit
AACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTT	5	0.125	No Hit
GGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATC	5	0.125	No Hit
GTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAG	5	0.125	No Hit
GGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAAC	5	0.125	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	5	0.125	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	5	0.125	No Hit
GGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGG	5	0.125	No Hit
CCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACAT	5	0.125	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	5	0.125	No Hit
GTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATT	5	0.125	No Hit
GTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGT	5	0.125	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	5	0.125	No Hit
CTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAA	5	0.125	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	5	0.125	No Hit
TGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTT	5	0.125	No Hit
CTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTA	5	0.125	No Hit
CAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATG	5	0.125	No Hit
GAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGG	5	0.125	No Hit
GTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTG	5	0.125	No Hit
CTGATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAG	5	0.125	No Hit
GCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCAT	5	0.125	No Hit
CAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAAT	5	0.125	No Hit
TAGTAACTTCCCTTACCCTGTACTTACAGTCTCGTACAGCACCTCACTGT	5	0.125	No Hit
CATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGC	5	0.125	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	5	0.125	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	5	0.125	No Hit
CCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.15000000000000002	0.0	0.0	0.0	0.0
78-79	0.25	0.0	0.0	0.0	0.0
80-81	0.3	0.0	0.0	0.0	0.0
82-83	0.4375	0.0	0.0	0.0	0.0
84-85	0.625	0.0	0.0	0.0	0.0
86-87	0.8	0.0	0.0	0.0	0.0
88-89	0.95	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGGGT	15	6.142176E-4	95.0	2
GGCAGGG	15	6.142176E-4	95.0	1
TAAAAGT	20	0.0019257745	71.25	8
AAAAGTT	25	0.0046641747	57.0	9
CCTACCT	15	0.009957196	47.5	26-27
GCCCAGG	15	0.009957196	47.5	10-11
TAAAAGC	15	0.009957196	47.5	18-19
TCCAGGA	15	0.009957196	47.5	64-65
AAAAGCC	15	0.009957196	47.5	20-21
GAGTAAA	15	0.009957196	47.5	16-17
AGTAAAA	15	0.009957196	47.5	16-17
TTCCAGG	15	0.009957196	47.5	64-65
CCTTCCA	15	0.009957196	47.5	62-63
AGTTGAG	15	0.009957196	47.5	12-13
ACCTTCC	15	0.009957196	47.5	28-29
TACCTTC	15	0.009957196	47.5	28-29
AAAGCCC	15	0.009957196	47.5	20-21
AAGCCCT	15	0.009957196	47.5	22-23
CTACCTT	15	0.009957196	47.5	26-27
CCCTACC	15	0.009957196	47.5	24-25
>>END_MODULE
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
Read 3648285 spots for SRR24882409.sra
Written 3648285 spots for SRR24882409.sra
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
Read 3648280 spots for SRR24882409.sra
Written 3648280 spots for SRR24882409.sra
SRR ids: ['SRR24882409.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_47k6nu6d
SRR24882409.sra spots: 29186245
blocks: [[1, 3648280], [3648281, 7296560], [7296561, 10944840], [10944841, 14593120], [14593121, 18241400], [18241401, 21889680], [21889681, 25537960], [25537961, 29186245]]
SRR24882409 file size 7046848
SRR24882409 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882409 SRR24882409_1.fastq SRR24882409_2.fastq
Input file:	SRR24882409_1.fastq
Paired file:	SRR24882409_2.fastq
trimmed:	SRR24882409-trimmed-pair1.fastq, SRR24882409-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 03:50:38 2026 >> started

Fri Jul  3 03:51:09 2026 >> done (30.948s)
29186245 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
29186245 (100.00%) read pairs available; of these:
  788604 ( 2.70%) trimmed read pairs available after processing
28397641 (97.30%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 64	       2	  0.00%
 65	       5	  0.00%
 66	      27	  0.00%
 67	     708	  0.00%
 68	    4832	  0.02%
 69	    5911	  0.02%
 70	    6917	  0.02%
 71	    7839	  0.03%
 72	    8449	  0.03%
 73	    9169	  0.03%
 74	    9772	  0.03%
 75	   11164	  0.04%
 76	   12122	  0.04%
 77	   13005	  0.04%
 78	   13660	  0.05%
 79	   14732	  0.05%
 80	   15772	  0.05%
 81	   17106	  0.06%
 82	   18069	  0.06%
 83	   19139	  0.07%
 84	   20911	  0.07%
 85	   21962	  0.08%
 86	   22760	  0.08%
 87	   24652	  0.08%
 88	   27127	  0.09%
 89	   27349	  0.09%
 90	   29562	  0.10%
 91	   31680	  0.11%
 92	   33140	  0.11%
 93	   35292	  0.12%
 94	   37148	  0.13%
 95	   39042	  0.13%
 96	   40288	  0.14%
 97	   44733	  0.15%
 98	   45563	  0.16%
 99	   49003	  0.17%
100	   69992	  0.24%
101	28397641	 97.30%
29186245 reads passed initial QC


criterion=sequence-density
sequence-density=0.71
sequence-density-rank=1
fanout-score=1.78
fanout-score-rank=36
prefix-density=1.25
prefix-fanout=1.0
sequence=GCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAGGGCACCGGGCAGGTCATCGAGGTGGCCTGCAGCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCATATTCAGCACCATGGCCACCAATCTTCCCCCAGGCAGCCTTGATGTTGCTTTTGTCTTCCCCAGAGAGCACCATGGTTTCTTCCTGAGTCTGTCA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=24
fanout-score=37.32
fanout-score-rank=1
prefix-density=0.73
prefix-fanout=1.2
sequence=TTGTCCAGATGAATTTACGAGCAGTCGGTGGATTAGTCGTCGGAGGGTCGTCAGTCATGCAGTGAAGCCAGCGGTGCCACTCAGGAGGCACCATGCTTCCATCCACATCCCAGAATGTGTTTTTGCCGTTCATCTCGGTGGTGTAGATGACCCATCGGTGGCGGCCAAAAAATTGCTTGTTG


criterion=sequence-density
sequence-density=0.44
sequence-density-rank=1
fanout-score=1.98
fanout-score-rank=34
prefix-density=0.44
prefix-fanout=2.0
sequence=TTGGGCAGAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=46.24
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=4.8
sequence=GGGAACAGAAAACTGCCCAGCTAAGCTCCCATAAGACTTCATACAGCTTCATGCTCTGCACTGTGACGATCACCGAAGCCAAGGTCTCCTAAAAGAAGACAGCTCACATTTGGCTGCCTGTTGTCAACTTTTTATCTTTCTCTTGACCACTTAGAAAAACTTGTCTCCCTACTTTTGCCTTCCTGGCATAATGGAAAAAGTGCAATACCTCACTCGCTCGGCTATCAGGAGAGCCTCCACTATTGAAATGCCTCAGCAAGCACGTCAGAATCTCCAGAACCTATTTATCAATTTCTGCCTCATCTTGATATGTCTGCTGCTGATCTGCATCATTGTGATGCTTCTGTGAAGAGCTGCCGCCACTCCAGACCTGCAACATGCCAACTCAGCTTAAAGCCGAGCACTCCGTCATGGGGGAAGCAACGCACCGCTGCCTTCCGGCTGAGAACAGCTTTGTGAGGGTCACGATTAAGAGTGAAACTAATTGTTAGCCAGTGTATTCATCTGCTGG
SRR24882409 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 03:51:20
                             Started mapping on |	Jul 03 03:51:21
                                    Finished on |	Jul 03 03:52:42
       Mapping speed, Million of reads per hour |	1297.17

                          Number of input reads |	29186245
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	23598866
                        Uniquely mapped reads % |	80.86%
                          Average mapped length |	200.59
                       Number of splices: Total |	12215036
            Number of splices: Annotated (sjdb) |	12118321
                       Number of splices: GT/AG |	12094602
                       Number of splices: GC/AG |	96212
                       Number of splices: AT/AC |	8526
               Number of splices: Non-canonical |	15696
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.69
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.55
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5579727
             % of reads mapped to multiple loci |	19.12%
        Number of reads mapped to too many loci |	3512
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	7652	7652	7652
N_multimapping	5579727	5579727	5579727
N_noFeature	1993910	21638586	2688141
N_ambiguous	1556197	559373	264081
UnstrandedReadsAssigned:20048759 PositiveStrandReadsAssigned:1400907 NegativeStrandReadsAssigned:20646644
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882409 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882409-trimmed-pair1.fastq
                             SRR24882409-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 29,186,245 reads, 24,468,891 reads pseudoaligned
[quant] estimated average fragment length: 188.933
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,514 rounds

 109283 SRR24882409.ke.tsv
  52636 SRR24882409.se.tsv
 161919 total
==> SRR24882409.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	12	0	0
ENSMUST00000196221.1	9	5	0	0
ENSMUST00000179664.1	11	7	0	0
ENSMUST00000178537.1	12	8	0	0
ENSMUST00000178862.1	14	10	0	0
ENSMUST00000179520.1	11	7	0	0
ENSMUST00000179883.1	16	12	0	0
ENSMUST00000195858.1	10	6	0	0
ENSMUST00000179932.1	12	8	0	0

==> SRR24882409.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	5
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882409 completed mapping pipeline successfully
