Starting /dee2/code/volunteer_pipeline.sh SRR24882427
    current disk space = 1325124788
    free memory = 198323836 
SRR24882427 SRAfilesize
d9de00471ce94cc3d26560a0785e6fbd  SRR24882427.sra
SRR24882427.sra file validated
SRR24882427 is paired end
SRR24882427 is conventional basespace
SRR24882427 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6185	37.0	37.0	37.0	37.0	37.0
2	36.555	37.0	37.0	37.0	37.0	37.0
3	36.49425	37.0	37.0	37.0	37.0	37.0
4	36.6865	37.0	37.0	37.0	37.0	37.0
5	36.6025	37.0	37.0	37.0	37.0	37.0
6	36.651	37.0	37.0	37.0	37.0	37.0
7	36.4925	37.0	37.0	37.0	37.0	37.0
8	36.5435	37.0	37.0	37.0	37.0	37.0
9	36.626	37.0	37.0	37.0	37.0	37.0
10-11	36.648375	37.0	37.0	37.0	37.0	37.0
12-13	36.64075	37.0	37.0	37.0	37.0	37.0
14-15	36.607	37.0	37.0	37.0	37.0	37.0
16-17	36.646	37.0	37.0	37.0	37.0	37.0
18-19	36.62675	37.0	37.0	37.0	37.0	37.0
20-21	36.631249999999994	37.0	37.0	37.0	37.0	37.0
22-23	36.65525	37.0	37.0	37.0	37.0	37.0
24-25	36.601	37.0	37.0	37.0	37.0	37.0
26-27	36.6065	37.0	37.0	37.0	37.0	37.0
28-29	36.57925	37.0	37.0	37.0	37.0	37.0
30-31	36.51025	37.0	37.0	37.0	37.0	37.0
32-33	36.528999999999996	37.0	37.0	37.0	37.0	37.0
34-35	36.5575	37.0	37.0	37.0	37.0	37.0
36-37	36.5405	37.0	37.0	37.0	37.0	37.0
38-39	36.46275	37.0	37.0	37.0	37.0	37.0
40-41	36.5355	37.0	37.0	37.0	37.0	37.0
42-43	36.479749999999996	37.0	37.0	37.0	37.0	37.0
44-45	36.51649999999999	37.0	37.0	37.0	37.0	37.0
46-47	36.514250000000004	37.0	37.0	37.0	37.0	37.0
48-49	36.420249999999996	37.0	37.0	37.0	37.0	37.0
50-51	36.48675	37.0	37.0	37.0	37.0	37.0
52-53	36.41975	37.0	37.0	37.0	37.0	37.0
54-55	36.465	37.0	37.0	37.0	37.0	37.0
56-57	36.41875	37.0	37.0	37.0	37.0	37.0
58-59	36.463	37.0	37.0	37.0	37.0	37.0
60-61	36.38075	37.0	37.0	37.0	37.0	37.0
62-63	36.48325	37.0	37.0	37.0	37.0	37.0
64-65	36.47525	37.0	37.0	37.0	37.0	37.0
66-67	36.417249999999996	37.0	37.0	37.0	37.0	37.0
68-69	36.35675	37.0	37.0	37.0	37.0	37.0
70-71	36.3855	37.0	37.0	37.0	37.0	37.0
72-73	36.38875	37.0	37.0	37.0	37.0	37.0
74-75	36.32425	37.0	37.0	37.0	37.0	37.0
76-77	36.32275	37.0	37.0	37.0	37.0	37.0
78-79	36.40175	37.0	37.0	37.0	37.0	37.0
80-81	36.358000000000004	37.0	37.0	37.0	37.0	37.0
82-83	36.361000000000004	37.0	37.0	37.0	37.0	37.0
84-85	36.35525	37.0	37.0	37.0	37.0	37.0
86-87	36.324749999999995	37.0	37.0	37.0	37.0	37.0
88-89	36.288	37.0	37.0	37.0	37.0	37.0
90-91	36.291	37.0	37.0	37.0	37.0	37.0
92-93	36.2355	37.0	37.0	37.0	37.0	37.0
94-95	36.210499999999996	37.0	37.0	37.0	37.0	37.0
96-97	36.1355	37.0	37.0	37.0	37.0	37.0
98-99	36.185249999999996	37.0	37.0	37.0	37.0	37.0
100-101	36.027249999999995	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	2.0
27	5.0
28	7.0
29	12.0
30	10.0
31	18.0
32	30.0
33	51.0
34	85.0
35	240.0
36	2748.0
37	790.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.75	11.025	11.175	43.05
2	17.083541770885443	18.234117058529264	36.11805902951476	28.564282141070535
3	18.598247809762203	21.97747183979975	32.41551939924906	27.008760951188986
4	23.724999999999998	27.975	25.15	23.150000000000002
5	24.4	32.275	22.85	20.474999999999998
6	18.8	34.5	28.825	17.875
7	13.925	25.25	44.725	16.1
8	20.05	23.400000000000002	32.300000000000004	24.25
9	18.15	26.700000000000003	33.2	21.95
10-11	20.06500812601575	35.904488061007626	24.090511313914238	19.939992499062384
12-13	21.587500000000002	28.1625	27.187499999999996	23.0625
14-15	20.275000000000002	31.825	27.800000000000004	20.1
16-17	19.75	29.599999999999998	26.787499999999998	23.8625
18-19	23.3	31.837500000000002	24.1875	20.674999999999997
20-21	21.349999999999998	28.512500000000003	27.3625	22.775000000000002
22-23	22.3125	29.1375	26.7125	21.837500000000002
24-25	23.7375	28.7	25.75	21.8125
26-27	20.875	29.799999999999997	28.275	21.05
28-29	20.150000000000002	30.4625	29.5875	19.8
30-31	21.425	28.4125	27.625	22.537499999999998
32-33	20.0375	27.250000000000004	30.912499999999998	21.8
34-35	18.8	28.499999999999996	28.275	24.425
36-37	21.775	28.3375	30.2	19.6875
38-39	20.6625	29.775000000000002	29.275000000000002	20.2875
40-41	20.125	31.3125	28.1375	20.424999999999997
42-43	20.6375	33.025	25.1875	21.15
44-45	19.650000000000002	31.7625	25.374999999999996	23.2125
46-47	22.75	32.15	22.85	22.25
48-49	20.175	32.35	25.937500000000004	21.5375
50-51	21.512500000000003	28.4125	27.3	22.775000000000002
52-53	20.8875	27.237499999999997	28.287499999999998	23.5875
54-55	20.1375	26.9125	28.1	24.85
56-57	16.8125	29.425	30.6875	23.075000000000003
58-59	20.7125	26.1	29.7875	23.400000000000002
60-61	19.8	30.4	27.6625	22.1375
62-63	22.375	28.725	28.799999999999997	20.1
64-65	21.25	26.85	27.6125	24.2875
66-67	23.05	27.85	28.425	20.674999999999997
68-69	21.275	29.175	28.1375	21.4125
70-71	23.025000000000002	29.875	27.537499999999998	19.5625
72-73	19.775000000000002	32.0625	26.5625	21.6
74-75	20.5375	29.2375	28.3125	21.912499999999998
76-77	23.525	29.512500000000003	26.1625	20.8
78-79	23.7625	28.9875	26.687499999999996	20.5625
80-81	24.525	27.1125	26.724999999999998	21.637500000000003
82-83	24.1625	29.462500000000002	27.3125	19.0625
84-85	19.9125	30.2875	26.575	23.225
86-87	21.4	28.712500000000002	27.35	22.537499999999998
88-89	26.400000000000002	26.887499999999996	28.1625	18.55
90-91	21.8875	28.037499999999998	29.262500000000003	20.8125
92-93	22.575	28.475	27.4125	21.5375
94-95	21.1125	30.012499999999996	28.0625	20.8125
96-97	19.950000000000003	29.275000000000002	24.1625	26.6125
98-99	20.3875	29.362500000000004	28.15	22.1
100-101	22.0125	28.875	27.375	21.7375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	1.5
21	1.5
22	7.5
23	11.5
24	7.0
25	15.0
26	21.0
27	11.0
28	4.0
29	10.0
30	41.5
31	85.0
32	150.5
33	176.5
34	149.0
35	157.5
36	158.0
37	125.5
38	68.5
39	53.0
40	86.5
41	152.0
42	253.0
43	234.5
44	157.5
45	145.0
46	208.0
47	259.5
48	195.5
49	129.5
50	122.0
51	123.0
52	83.5
53	48.5
54	47.0
55	49.5
56	48.0
57	34.0
58	50.5
59	101.5
60	92.0
61	35.5
62	17.0
63	12.0
64	3.0
65	1.5
66	4.0
67	9.5
68	7.0
69	4.0
70	3.0
71	1.5
72	3.5
73	3.5
74	6.5
75	5.5
76	2.0
77	2.0
78	1.0
79	0.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05
3	0.125
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0125
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.49675558759913	18.55
2	17.519826964671953	12.15
3	8.29127613554434	8.625
4	4.974765681326604	6.9
5	3.316510454217736	5.75
6	2.883922134102379	6.0
7	1.658255227108868	4.025
8	1.36986301369863	3.8
9	0.7930785868781542	2.475
>10	5.5515501081470795	28.425
>50	0.14419610670511895	3.3000000000000003
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	73	1.825	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	59	1.4749999999999999	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	41	1.0250000000000001	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	37	0.9249999999999999	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	31	0.775	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	29	0.7250000000000001	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	27	0.675	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	26	0.65	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	24	0.6	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	22	0.5499999999999999	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	21	0.525	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	20	0.5	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	20	0.5	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	19	0.475	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	19	0.475	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	19	0.475	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	18	0.44999999999999996	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	18	0.44999999999999996	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	18	0.44999999999999996	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	17	0.42500000000000004	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	17	0.42500000000000004	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	16	0.4	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	16	0.4	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	16	0.4	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	16	0.4	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	15	0.375	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	15	0.375	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	15	0.375	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	14	0.35000000000000003	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	14	0.35000000000000003	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	14	0.35000000000000003	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	14	0.35000000000000003	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	14	0.35000000000000003	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	14	0.35000000000000003	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	13	0.325	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	13	0.325	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	13	0.325	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	13	0.325	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	13	0.325	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	13	0.325	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	12	0.3	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	12	0.3	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	12	0.3	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	12	0.3	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	12	0.3	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	12	0.3	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	12	0.3	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	12	0.3	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	12	0.3	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	12	0.3	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	12	0.3	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	11	0.27499999999999997	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	11	0.27499999999999997	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	11	0.27499999999999997	No Hit
GTCAATTTCTACATCTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGAC	11	0.27499999999999997	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	11	0.27499999999999997	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	11	0.27499999999999997	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	11	0.27499999999999997	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	11	0.27499999999999997	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	11	0.27499999999999997	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	11	0.27499999999999997	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	11	0.27499999999999997	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	10	0.25	No Hit
AATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTT	10	0.25	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	10	0.25	No Hit
CACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTAT	10	0.25	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	10	0.25	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	10	0.25	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	10	0.25	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	10	0.25	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	10	0.25	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	10	0.25	No Hit
GTCCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCC	10	0.25	No Hit
GTTCTTTTAAAAAATAAAATATGTTCAAAGCACCCTTGATATAAATTGCC	10	0.25	No Hit
CAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGAT	10	0.25	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	10	0.25	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	10	0.25	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	10	0.25	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	10	0.25	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	9	0.22499999999999998	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	9	0.22499999999999998	No Hit
CCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAAT	9	0.22499999999999998	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	9	0.22499999999999998	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	9	0.22499999999999998	No Hit
CTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCT	9	0.22499999999999998	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	9	0.22499999999999998	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	9	0.22499999999999998	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	9	0.22499999999999998	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	9	0.22499999999999998	No Hit
CTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATAT	9	0.22499999999999998	No Hit
ACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGG	8	0.2	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	8	0.2	No Hit
GGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCA	8	0.2	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	8	0.2	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	8	0.2	No Hit
GTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGGA	8	0.2	No Hit
GTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTG	8	0.2	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	8	0.2	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	8	0.2	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	8	0.2	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	8	0.2	No Hit
CTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGAACAT	8	0.2	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	8	0.2	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	8	0.2	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	8	0.2	No Hit
CAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATT	8	0.2	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	8	0.2	No Hit
TCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCCTTGACCAACTC	8	0.2	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	8	0.2	No Hit
CTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGC	7	0.17500000000000002	No Hit
AGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAAT	7	0.17500000000000002	No Hit
CGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGG	7	0.17500000000000002	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	7	0.17500000000000002	No Hit
GGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAAC	7	0.17500000000000002	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	7	0.17500000000000002	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	7	0.17500000000000002	No Hit
GGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGC	7	0.17500000000000002	No Hit
CAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATG	7	0.17500000000000002	No Hit
AAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGA	7	0.17500000000000002	No Hit
CTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAA	7	0.17500000000000002	No Hit
CTCAACCAATGAAATAATAAAATTTATGAATTAAGCCCTTGACCAACTCA	7	0.17500000000000002	No Hit
ACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAA	7	0.17500000000000002	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	7	0.17500000000000002	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	7	0.17500000000000002	No Hit
GCGCCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	7	0.17500000000000002	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	7	0.17500000000000002	No Hit
AGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAAC	7	0.17500000000000002	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	7	0.17500000000000002	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	7	0.17500000000000002	No Hit
AAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGGAAA	7	0.17500000000000002	No Hit
TGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCT	7	0.17500000000000002	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	7	0.17500000000000002	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	6	0.15	No Hit
TGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATAC	6	0.15	No Hit
GTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACAC	6	0.15	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	6	0.15	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	6	0.15	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	6	0.15	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	6	0.15	No Hit
GTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATG	6	0.15	No Hit
CCGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	6	0.15	No Hit
CGCCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAA	6	0.15	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	6	0.15	No Hit
GGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACATCC	6	0.15	No Hit
CCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGC	6	0.15	No Hit
CTTGTAAATAAATCATTGGTAAACATTACAAAATTAAATACATTTTCAAA	6	0.15	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	6	0.15	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	6	0.15	No Hit
CATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACT	6	0.15	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	6	0.15	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	6	0.15	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	6	0.15	No Hit
AAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACA	6	0.15	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	6	0.15	No Hit
TAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACA	6	0.15	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	6	0.15	No Hit
CCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGT	6	0.15	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	6	0.15	No Hit
CACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTC	6	0.15	No Hit
GAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATT	6	0.15	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	6	0.15	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	6	0.15	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	6	0.15	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	6	0.15	No Hit
AATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACATATGCAGTAT	6	0.15	No Hit
CTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAA	6	0.15	No Hit
TGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCT	6	0.15	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	6	0.15	No Hit
GTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAAC	6	0.15	No Hit
AGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGC	6	0.15	No Hit
GAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTC	6	0.15	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	6	0.15	No Hit
CTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTC	5	0.125	No Hit
TATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAA	5	0.125	No Hit
TCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAA	5	0.125	No Hit
ATAAAATATGTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTT	5	0.125	No Hit
ATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTC	5	0.125	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	5	0.125	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	5	0.125	No Hit
TATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACACTTTT	5	0.125	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	5	0.125	No Hit
GACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCT	5	0.125	No Hit
ACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGC	5	0.125	No Hit
ATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTTTTT	5	0.125	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	5	0.125	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	5	0.125	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	5	0.125	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	5	0.125	No Hit
GCGCCTAGACGCTGGATCCTTGCGGAGGGAGGCGGGAAGAGGGCGGGCGA	5	0.125	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	5	0.125	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	5	0.125	No Hit
GTTTGTTTACATAATGCAGCCAGCCATGACTGCCAAACACAATGGTTAAG	5	0.125	No Hit
TACAACTTTAAAAAACCACAATGAGGCAATAAGTACATGACCTGGCAAAG	5	0.125	No Hit
CGCCATTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAA	5	0.125	No Hit
GTCTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACA	5	0.125	No Hit
GGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTA	5	0.125	No Hit
AGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGT	5	0.125	No Hit
TCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAAT	5	0.125	No Hit
TTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGA	5	0.125	No Hit
CCACAATGAGGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGA	5	0.125	No Hit
GACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGT	5	0.125	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	5	0.125	No Hit
CTGTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTA	5	0.125	No Hit
GTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTG	5	0.125	No Hit
GTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGC	5	0.125	No Hit
ATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACATATGCAGTATG	5	0.125	No Hit
CAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACTGAA	5	0.125	No Hit
GGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTT	5	0.125	No Hit
CTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAG	5	0.125	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	5	0.125	No Hit
CTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCC	5	0.125	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	5	0.125	No Hit
CGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGG	5	0.125	No Hit
TGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTT	5	0.125	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	5	0.125	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	5	0.125	No Hit
GAAATAATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACATATGC	5	0.125	No Hit
GTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.0875	0.0	0.0	0.0	0.0
72-73	0.1125	0.0	0.0	0.0	0.0
74-75	0.15	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.2	0.0	0.0	0.0	0.0
80-81	0.2	0.0	0.0	0.0	0.0
82-83	0.225	0.0	0.0	0.0	0.0
84-85	0.375	0.0	0.0	0.0	0.0
86-87	0.55	0.0	0.0	0.0	0.0
88-89	0.575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882427 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882427_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.376	37.0	37.0	37.0	37.0	37.0
2	36.3805	37.0	37.0	37.0	37.0	37.0
3	36.426	37.0	37.0	37.0	37.0	37.0
4	36.3845	37.0	37.0	37.0	37.0	37.0
5	36.3425	37.0	37.0	37.0	37.0	37.0
6	36.411	37.0	37.0	37.0	37.0	37.0
7	36.442	37.0	37.0	37.0	37.0	37.0
8	36.4415	37.0	37.0	37.0	37.0	37.0
9	36.4525	37.0	37.0	37.0	37.0	37.0
10-11	36.45425	37.0	37.0	37.0	37.0	37.0
12-13	36.477000000000004	37.0	37.0	37.0	37.0	37.0
14-15	36.323750000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.4045	37.0	37.0	37.0	37.0	37.0
18-19	36.419250000000005	37.0	37.0	37.0	37.0	37.0
20-21	36.37875	37.0	37.0	37.0	37.0	37.0
22-23	36.326750000000004	37.0	37.0	37.0	37.0	37.0
24-25	36.334999999999994	37.0	37.0	37.0	37.0	37.0
26-27	36.39425	37.0	37.0	37.0	37.0	37.0
28-29	36.3595	37.0	37.0	37.0	37.0	37.0
30-31	36.3125	37.0	37.0	37.0	37.0	37.0
32-33	36.24825	37.0	37.0	37.0	37.0	37.0
34-35	36.24025	37.0	37.0	37.0	37.0	37.0
36-37	36.3275	37.0	37.0	37.0	37.0	37.0
38-39	36.27525	37.0	37.0	37.0	37.0	37.0
40-41	36.2855	37.0	37.0	37.0	37.0	37.0
42-43	36.225	37.0	37.0	37.0	37.0	37.0
44-45	36.2535	37.0	37.0	37.0	37.0	37.0
46-47	36.210750000000004	37.0	37.0	37.0	37.0	37.0
48-49	36.0865	37.0	37.0	37.0	37.0	37.0
50-51	36.14	37.0	37.0	37.0	37.0	37.0
52-53	36.194500000000005	37.0	37.0	37.0	37.0	37.0
54-55	36.12025	37.0	37.0	37.0	37.0	37.0
56-57	36.09375	37.0	37.0	37.0	37.0	37.0
58-59	36.14425	37.0	37.0	37.0	37.0	37.0
60-61	36.06375	37.0	37.0	37.0	37.0	37.0
62-63	36.00625	37.0	37.0	37.0	37.0	37.0
64-65	36.11475	37.0	37.0	37.0	37.0	37.0
66-67	35.97825	37.0	37.0	37.0	37.0	37.0
68-69	36.0585	37.0	37.0	37.0	37.0	37.0
70-71	36.074	37.0	37.0	37.0	37.0	37.0
72-73	35.98375	37.0	37.0	37.0	37.0	37.0
74-75	35.9385	37.0	37.0	37.0	37.0	37.0
76-77	36.0255	37.0	37.0	37.0	37.0	37.0
78-79	35.923249999999996	37.0	37.0	37.0	37.0	37.0
80-81	35.855500000000006	37.0	37.0	37.0	37.0	37.0
82-83	35.8695	37.0	37.0	37.0	37.0	37.0
84-85	35.716499999999996	37.0	37.0	37.0	37.0	37.0
86-87	35.85325	37.0	37.0	37.0	37.0	37.0
88-89	35.75925	37.0	37.0	37.0	37.0	37.0
90-91	35.704499999999996	37.0	37.0	37.0	37.0	37.0
92-93	35.68925	37.0	37.0	37.0	37.0	37.0
94-95	35.743	37.0	37.0	37.0	37.0	37.0
96-97	35.751000000000005	37.0	37.0	37.0	37.0	37.0
98-99	35.7665	37.0	37.0	37.0	37.0	37.0
100-101	35.4285	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	4.0
25	2.0
26	8.0
27	9.0
28	10.0
29	14.0
30	17.0
31	21.0
32	38.0
33	77.0
34	161.0
35	547.0
36	2788.0
37	303.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.92230576441102	19.849624060150376	14.661654135338345	26.56641604010025
2	21.25	27.575	34.775	16.400000000000002
3	20.65	26.900000000000002	32.525	19.925
4	22.85	32.95	24.15	20.05
5	23.724999999999998	32.025	26.55	17.7
6	23.325000000000003	34.0	23.849999999999998	18.825
7	21.175	22.35	38.45	18.025
8	24.7	22.125	30.2	22.975
9	23.375	24.975	28.249999999999996	23.400000000000002
10-11	24.3625	31.0375	23.875	20.724999999999998
12-13	25.775	24.349999999999998	27.950000000000003	21.925
14-15	24.0625	24.85	31.574999999999996	19.5125
16-17	23.225	27.537499999999998	31.724999999999998	17.5125
18-19	22.7125	28.037499999999998	28.787499999999998	20.4625
20-21	22.9875	29.825000000000003	28.499999999999996	18.6875
22-23	22.3875	29.15	28.8625	19.6
24-25	24.3125	28.999999999999996	27.0875	19.6
26-27	23.962500000000002	25.4375	29.325000000000003	21.275
28-29	23.525	27.8125	28.9375	19.725
30-31	22.8125	27.0	29.775000000000002	20.4125
32-33	24.0625	26.187500000000004	30.95	18.8
34-35	23.8125	27.537499999999998	27.3	21.349999999999998
36-37	21.125	28.212500000000002	30.5	20.1625
38-39	22.275	26.7125	28.3125	22.7
40-41	21.7	28.0875	29.7125	20.5
42-43	22.3375	28.3625	29.1875	20.1125
44-45	21.5	28.787499999999998	29.6875	20.025000000000002
46-47	22.2625	28.525	26.387500000000003	22.825
48-49	21.2375	26.3625	30.912499999999998	21.4875
50-51	21.75	27.450000000000003	27.8375	22.9625
52-53	23.075000000000003	27.6125	28.8625	20.45
54-55	24.5375	27.900000000000002	27.075	20.4875
56-57	20.175	28.875	28.475	22.475
58-59	19.6	29.075	29.025000000000002	22.3
60-61	20.775	27.800000000000004	30.525000000000002	20.9
62-63	22.2125	29.599999999999998	26.937499999999996	21.25
64-65	22.125	27.0125	31.4	19.4625
66-67	20.474999999999998	25.674999999999997	30.25	23.599999999999998
68-69	21.6875	29.2875	28.249999999999996	20.775
70-71	21.0375	29.175	29.9	19.8875
72-73	21.85	28.275	28.349999999999998	21.525
74-75	23.05	27.075	29.562500000000004	20.3125
76-77	21.837500000000002	27.9375	29.025000000000002	21.2
78-79	22.2125	27.950000000000003	28.775000000000002	21.0625
80-81	21.6875	28.5875	29.725	20.0
82-83	20.974999999999998	27.0	30.85	21.175
84-85	20.0	27.025	31.775	21.2
86-87	21.6875	27.8375	28.9375	21.5375
88-89	23.5	27.925	28.8375	19.7375
90-91	24.15	27.450000000000003	26.200000000000003	22.2
92-93	21.637500000000003	29.3875	28.8875	20.0875
94-95	20.724999999999998	30.95	27.8125	20.5125
96-97	22.475	28.15	27.9125	21.462500000000002
98-99	22.5125	28.3875	28.075	21.025
100-101	23.1875	28.025	28.6125	20.175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	1.5
24	2.0
25	2.5
26	3.5
27	3.0
28	3.5
29	15.0
30	47.0
31	140.5
32	186.0
33	147.5
34	137.5
35	115.5
36	103.0
37	120.0
38	113.0
39	91.0
40	117.0
41	178.5
42	237.5
43	263.5
44	246.5
45	241.5
46	210.0
47	190.5
48	164.5
49	105.0
50	91.0
51	91.0
52	73.0
53	46.5
54	53.5
55	100.0
56	73.5
57	17.5
58	35.0
59	43.5
60	26.0
61	16.5
62	17.5
63	10.5
64	4.5
65	4.0
66	0.5
67	3.0
68	4.0
69	7.5
70	8.0
71	9.5
72	12.0
73	7.5
74	11.5
75	12.0
76	9.0
77	12.0
78	8.5
79	2.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.07219251336899	19.475
2	17.780748663101605	13.3
3	8.689839572192513	9.75
4	5.748663101604278	8.6
5	4.612299465240642	8.625
6	2.874331550802139	6.45
7	1.9385026737967916	5.075
8	1.537433155080214	4.6
9	0.9358288770053476	3.15
>10	3.7433155080213902	19.650000000000002
>50	0.06684491978609625	1.325
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	53	1.325	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	34	0.8500000000000001	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	28	0.7000000000000001	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	23	0.575	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	22	0.5499999999999999	No Hit
GGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTC	19	0.475	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	19	0.475	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	18	0.44999999999999996	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	18	0.44999999999999996	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	18	0.44999999999999996	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	17	0.42500000000000004	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	17	0.42500000000000004	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	17	0.42500000000000004	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	16	0.4	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	15	0.375	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	15	0.375	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	15	0.375	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	14	0.35000000000000003	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	14	0.35000000000000003	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	14	0.35000000000000003	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	14	0.35000000000000003	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	14	0.35000000000000003	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	14	0.35000000000000003	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	14	0.35000000000000003	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	14	0.35000000000000003	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	14	0.35000000000000003	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	14	0.35000000000000003	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	13	0.325	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	13	0.325	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	13	0.325	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	13	0.325	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	13	0.325	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	12	0.3	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	12	0.3	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	12	0.3	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	12	0.3	No Hit
ACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTG	11	0.27499999999999997	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	11	0.27499999999999997	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	11	0.27499999999999997	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	11	0.27499999999999997	No Hit
AGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGG	11	0.27499999999999997	No Hit
TATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCT	11	0.27499999999999997	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	11	0.27499999999999997	No Hit
TTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACAC	11	0.27499999999999997	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	11	0.27499999999999997	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	11	0.27499999999999997	No Hit
CACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGT	11	0.27499999999999997	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	11	0.27499999999999997	No Hit
TTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTG	10	0.25	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	10	0.25	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	10	0.25	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	10	0.25	No Hit
ATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTCC	10	0.25	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	10	0.25	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	10	0.25	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	10	0.25	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	10	0.25	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	9	0.22499999999999998	No Hit
CATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTC	9	0.22499999999999998	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	9	0.22499999999999998	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	9	0.22499999999999998	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	9	0.22499999999999998	No Hit
AGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGA	9	0.22499999999999998	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	9	0.22499999999999998	No Hit
TGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAAT	9	0.22499999999999998	No Hit
GGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGA	9	0.22499999999999998	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	9	0.22499999999999998	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	9	0.22499999999999998	No Hit
CAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTA	9	0.22499999999999998	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	9	0.22499999999999998	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	9	0.22499999999999998	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	8	0.2	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	8	0.2	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	8	0.2	No Hit
CACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAA	8	0.2	No Hit
AGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCT	8	0.2	No Hit
CATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAA	8	0.2	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	8	0.2	No Hit
CTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCA	8	0.2	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	8	0.2	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	8	0.2	No Hit
GGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGA	8	0.2	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	8	0.2	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	8	0.2	No Hit
CACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAA	8	0.2	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	8	0.2	No Hit
GCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAA	8	0.2	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	8	0.2	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	8	0.2	No Hit
GAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAA	8	0.2	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	8	0.2	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	8	0.2	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	8	0.2	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	8	0.2	No Hit
GTCAAAAGGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGG	7	0.17500000000000002	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	7	0.17500000000000002	No Hit
ATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGT	7	0.17500000000000002	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	7	0.17500000000000002	No Hit
TTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTTG	7	0.17500000000000002	No Hit
CATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACA	7	0.17500000000000002	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	7	0.17500000000000002	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	7	0.17500000000000002	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	7	0.17500000000000002	No Hit
CCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAA	7	0.17500000000000002	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	7	0.17500000000000002	No Hit
AAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAG	7	0.17500000000000002	No Hit
TGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACA	7	0.17500000000000002	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	7	0.17500000000000002	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	7	0.17500000000000002	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	7	0.17500000000000002	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	7	0.17500000000000002	No Hit
GAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGG	7	0.17500000000000002	No Hit
ACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGG	7	0.17500000000000002	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	7	0.17500000000000002	No Hit
AGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTC	7	0.17500000000000002	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	7	0.17500000000000002	No Hit
AGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGA	7	0.17500000000000002	No Hit
GCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGAT	7	0.17500000000000002	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	7	0.17500000000000002	No Hit
AAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGC	7	0.17500000000000002	No Hit
GAAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGT	7	0.17500000000000002	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	7	0.17500000000000002	No Hit
GCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTAC	7	0.17500000000000002	No Hit
ATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTG	6	0.15	No Hit
TGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCT	6	0.15	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	6	0.15	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	6	0.15	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	6	0.15	No Hit
CTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGT	6	0.15	No Hit
GTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAG	6	0.15	No Hit
TGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAG	6	0.15	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	6	0.15	No Hit
GGCGGCCGGTGGCGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTC	6	0.15	No Hit
GATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCT	6	0.15	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	6	0.15	No Hit
TGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACAC	6	0.15	No Hit
TAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTAC	6	0.15	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	6	0.15	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	6	0.15	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	6	0.15	No Hit
GTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGT	6	0.15	No Hit
CAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTT	6	0.15	No Hit
AGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCTCCA	6	0.15	No Hit
GGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTC	6	0.15	No Hit
AAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAG	6	0.15	No Hit
CCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTT	6	0.15	No Hit
ACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTC	6	0.15	No Hit
AAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAA	6	0.15	No Hit
GAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTG	6	0.15	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	6	0.15	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	6	0.15	No Hit
TTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTAT	6	0.15	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	6	0.15	No Hit
GTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACC	6	0.15	No Hit
GTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCT	6	0.15	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	6	0.15	No Hit
GTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTA	6	0.15	No Hit
ATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCA	6	0.15	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	6	0.15	No Hit
AATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTT	6	0.15	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	6	0.15	No Hit
CTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTCTC	6	0.15	No Hit
ACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAA	6	0.15	No Hit
CTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTT	6	0.15	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	6	0.15	No Hit
TCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTT	6	0.15	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	5	0.125	No Hit
AGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATT	5	0.125	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	5	0.125	No Hit
CTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTG	5	0.125	No Hit
AAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAG	5	0.125	No Hit
AAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGA	5	0.125	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	5	0.125	No Hit
GCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAA	5	0.125	No Hit
CTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCAT	5	0.125	No Hit
CCTCAAGGGAGGACTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAA	5	0.125	No Hit
ACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAA	5	0.125	No Hit
AAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGC	5	0.125	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	5	0.125	No Hit
GTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTT	5	0.125	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	5	0.125	No Hit
AGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAG	5	0.125	No Hit
AGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAG	5	0.125	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	5	0.125	No Hit
ATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCT	5	0.125	No Hit
GGAGTCGACATATTTCGTGCGCCCCAAGATTGCTCGAGAAGCGCAGTTCG	5	0.125	No Hit
GGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAG	5	0.125	No Hit
AGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTT	5	0.125	No Hit
TAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTC	5	0.125	No Hit
AATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGT	5	0.125	No Hit
CTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTC	5	0.125	No Hit
ATGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACA	5	0.125	No Hit
CTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGA	5	0.125	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	5	0.125	No Hit
GTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGG	5	0.125	No Hit
GGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTT	5	0.125	No Hit
CTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGC	5	0.125	No Hit
GTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAA	5	0.125	No Hit
ACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTC	5	0.125	No Hit
CTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAGCCCCTGCTGCCTCCC	5	0.125	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	5	0.125	No Hit
GGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAA	5	0.125	No Hit
ACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCA	5	0.125	No Hit
GATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGC	5	0.125	No Hit
CAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATG	5	0.125	No Hit
GCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCT	5	0.125	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	5	0.125	No Hit
CCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTAC	5	0.125	No Hit
TCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTA	5	0.125	No Hit
CTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTG	5	0.125	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	5	0.125	No Hit
GTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTT	5	0.125	No Hit
TTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGAT	5	0.125	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	5	0.125	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	5	0.125	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	5	0.125	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	5	0.125	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	5	0.125	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	5	0.125	No Hit
CTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTC	5	0.125	No Hit
GTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTG	5	0.125	No Hit
CAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATT	5	0.125	No Hit
GTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTT	5	0.125	No Hit
GACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAA	5	0.125	No Hit
CTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGT	5	0.125	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	5	0.125	No Hit
GCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCAT	5	0.125	No Hit
CAGGCTCTGGCGCGCGGGGACATGTCGGTGGTGACTGGCGGCGGTGAGGC	5	0.125	No Hit
GACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTT	5	0.125	No Hit
ATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTT	5	0.125	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	5	0.125	No Hit
GCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATA	5	0.125	No Hit
TGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	5	0.125	No Hit
GAAAGAACACCATGGATGGATAAAGGAAGATAACAATAACTAGAATAAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.0875	0.0	0.0	0.0	0.0
72-73	0.1125	0.0	0.0	0.0	0.0
74-75	0.15	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.2	0.0	0.0	0.0	0.0
80-81	0.2	0.0	0.0	0.0	0.0
82-83	0.21250000000000002	0.0	0.0	0.0	0.0
84-85	0.325	0.0	0.0	0.0	0.0
86-87	0.475	0.0	0.0	0.0	0.0
88-89	0.5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
Read 4767963 spots for SRR24882427.sra
Written 4767963 spots for SRR24882427.sra
SRR ids: ['SRR24882427.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_iww1w_ac
SRR24882427.sra spots: 38143704
blocks: [[1, 4767963], [4767964, 9535926], [9535927, 14303889], [14303890, 19071852], [19071853, 23839815], [23839816, 28607778], [28607779, 33375741], [33375742, 38143704]]
SRR24882427 file size 9216232
SRR24882427 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882427 SRR24882427_1.fastq SRR24882427_2.fastq
Input file:	SRR24882427_1.fastq
Paired file:	SRR24882427_2.fastq
trimmed:	SRR24882427-trimmed-pair1.fastq, SRR24882427-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 03:20:36 2026 >> started

Fri Jul  3 03:21:16 2026 >> done (39.565s)
38143704 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
38143704 (100.00%) read pairs available; of these:
  596917 ( 1.56%) trimmed read pairs available after processing
37546787 (98.44%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 66	      27	  0.00%
 67	     814	  0.00%
 68	    4219	  0.01%
 69	    5173	  0.01%
 70	    5788	  0.02%
 71	    6657	  0.02%
 72	    7347	  0.02%
 73	    7813	  0.02%
 74	    8276	  0.02%
 75	    8897	  0.02%
 76	    9726	  0.03%
 77	   10579	  0.03%
 78	   11258	  0.03%
 79	   11841	  0.03%
 80	   12564	  0.03%
 81	   13249	  0.03%
 82	   14154	  0.04%
 83	   15225	  0.04%
 84	   16261	  0.04%
 85	   17213	  0.05%
 86	   17295	  0.05%
 87	   18871	  0.05%
 88	   20135	  0.05%
 89	   20167	  0.05%
 90	   22537	  0.06%
 91	   23759	  0.06%
 92	   24480	  0.06%
 93	   25181	  0.07%
 94	   26661	  0.07%
 95	   27899	  0.07%
 96	   29431	  0.08%
 97	   31896	  0.08%
 98	   32594	  0.09%
 99	   35484	  0.09%
100	   53446	  0.14%
101	37546787	 98.44%
38143704 reads passed initial QC


criterion=sequence-density
sequence-density=0.52
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=33
prefix-density=0.51
prefix-fanout=2.0
sequence=GATGGGAACAGGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=25.47
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=3.7
sequence=TTTACTTTCTTCAAAGTTAGGAGGAAAGAAAGAAAGTGACCACACTTGGGGGCCTGGGAGAAAACCAGAAAGTACTATCCATTTTCAATCAGATTTCAAAGCAGTGGGTGTGTCTAGAAGCAATGCCCTGCCCTCAGTATTCCTCGGGATTGCTTGATTGTAAGTCTCGCAAATAGGAATCAATTATGTTTGGAGGCACGCCTTGCAGGAGGAGCCTGCAGAAGGACAGCCCACCTTGGGTGACAATATACATTCAAACATCCTTACTGTGCTACGACTTTCACCTCCAGTTTGCATTCAATCTCCACCGTCCCGAGGTCATTGACTGCTTTGCAGCAGTACGTGCCTCCGTCATAAGGACTAGGCTTGCGGATCTCCAGGGTACAGACTCCTTGGTTGCTGAACATTCTGTATCTTGGGTCATCCACAATAGCCACTTTGTTTTTCATCCAGGTGATTTTGGGCTTGGGATTTCCTCGGACGCTGCAGTTCAGGGTGGCATTGTAACCAG


criterion=sequence-density
sequence-density=0.22
sequence-density-rank=1
fanout-score=2.66
fanout-score-rank=26
prefix-density=0.25
prefix-fanout=2.4
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=25
fanout-score=49.06
fanout-score-rank=1
prefix-density=0.43
prefix-fanout=8.5
sequence=CCTGGAGGAGGACCATGCCTGCATCCCCATCAAGAAATCTGACCCTGTTGTGTCATATCGGGAGACAGTCAGTGAGGAATCCAACGTGCTGTGTCTGTCCAAGTCCCCCAATAAGCACAACCGGCTGTACATGAAGGCCAGGCCCTTCCCTGATGGCCTGGCAGAGGACATCGATAAGGGTGAGGTGTCTGC
SRR24882427 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 03:21:31
                             Started mapping on |	Jul 03 03:21:31
                                    Finished on |	Jul 03 03:39:34
       Mapping speed, Million of reads per hour |	126.79

                          Number of input reads |	38143704
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	33949160
                        Uniquely mapped reads % |	89.00%
                          Average mapped length |	200.74
                       Number of splices: Total |	24200868
            Number of splices: Annotated (sjdb) |	24013320
                       Number of splices: GT/AG |	24024820
                       Number of splices: GC/AG |	140770
                       Number of splices: AT/AC |	14032
               Number of splices: Non-canonical |	21246
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.60
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.60
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4188978
             % of reads mapped to multiple loci |	10.98%
        Number of reads mapped to too many loci |	2443
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5566	5566	5566
N_multimapping	4188978	4188978	4188978
N_noFeature	2274398	30235447	3229752
N_ambiguous	3072636	1092848	297378
UnstrandedReadsAssigned:28602126 PositiveStrandReadsAssigned:2620865 NegativeStrandReadsAssigned:30422030
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882427 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882427-trimmed-pair1.fastq
                             SRR24882427-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 38,143,704 reads, 32,278,725 reads pseudoaligned
[quant] estimated average fragment length: 212.532
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,275 rounds

 109283 SRR24882427.ke.tsv
  52636 SRR24882427.se.tsv
 161919 total
==> SRR24882427.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8.22222	0	0
ENSMUST00000196221.1	9	4	0	0
ENSMUST00000179664.1	11	5.28571	0	0
ENSMUST00000178537.1	12	6.28571	0	0
ENSMUST00000178862.1	14	8.28571	0	0
ENSMUST00000179520.1	11	5.28571	0	0
ENSMUST00000179883.1	16	8.22222	0	0
ENSMUST00000195858.1	10	5	0	0
ENSMUST00000179932.1	12	6.28571	0	0

==> SRR24882427.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	6
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882427 completed mapping pipeline successfully
