Starting /dee2/code/volunteer_pipeline.sh SRR24882426
    current disk space = 1398484448
    free memory = 200425916 
SRR24882426 SRAfilesize
ed1722f1bdb57a8b369110ac504e0625  SRR24882426.sra
SRR24882426.sra file validated
SRR24882426 is paired end
SRR24882426 is conventional basespace
SRR24882426 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4705	37.0	37.0	37.0	37.0	37.0
2	36.43375	37.0	37.0	37.0	37.0	37.0
3	36.529	37.0	37.0	37.0	37.0	37.0
4	36.529	37.0	37.0	37.0	37.0	37.0
5	36.5815	37.0	37.0	37.0	37.0	37.0
6	36.656	37.0	37.0	37.0	37.0	37.0
7	36.568	37.0	37.0	37.0	37.0	37.0
8	36.6055	37.0	37.0	37.0	37.0	37.0
9	36.5965	37.0	37.0	37.0	37.0	37.0
10-11	36.619749999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.6195	37.0	37.0	37.0	37.0	37.0
14-15	36.61775	37.0	37.0	37.0	37.0	37.0
16-17	36.62325	37.0	37.0	37.0	37.0	37.0
18-19	36.603750000000005	37.0	37.0	37.0	37.0	37.0
20-21	36.617999999999995	37.0	37.0	37.0	37.0	37.0
22-23	36.62925	37.0	37.0	37.0	37.0	37.0
24-25	36.6225	37.0	37.0	37.0	37.0	37.0
26-27	36.56925	37.0	37.0	37.0	37.0	37.0
28-29	36.566500000000005	37.0	37.0	37.0	37.0	37.0
30-31	36.520250000000004	37.0	37.0	37.0	37.0	37.0
32-33	36.529250000000005	37.0	37.0	37.0	37.0	37.0
34-35	36.522999999999996	37.0	37.0	37.0	37.0	37.0
36-37	36.432249999999996	37.0	37.0	37.0	37.0	37.0
38-39	36.509249999999994	37.0	37.0	37.0	37.0	37.0
40-41	36.45025	37.0	37.0	37.0	37.0	37.0
42-43	36.4895	37.0	37.0	37.0	37.0	37.0
44-45	36.4175	37.0	37.0	37.0	37.0	37.0
46-47	36.52525	37.0	37.0	37.0	37.0	37.0
48-49	36.36825	37.0	37.0	37.0	37.0	37.0
50-51	36.42725	37.0	37.0	37.0	37.0	37.0
52-53	36.47775	37.0	37.0	37.0	37.0	37.0
54-55	36.4395	37.0	37.0	37.0	37.0	37.0
56-57	36.329750000000004	37.0	37.0	37.0	37.0	37.0
58-59	36.46575	37.0	37.0	37.0	37.0	37.0
60-61	36.341750000000005	37.0	37.0	37.0	37.0	37.0
62-63	36.414249999999996	37.0	37.0	37.0	37.0	37.0
64-65	36.35275	37.0	37.0	37.0	37.0	37.0
66-67	36.36875	37.0	37.0	37.0	37.0	37.0
68-69	36.2825	37.0	37.0	37.0	37.0	37.0
70-71	36.278000000000006	37.0	37.0	37.0	37.0	37.0
72-73	36.3575	37.0	37.0	37.0	37.0	37.0
74-75	36.31275	37.0	37.0	37.0	37.0	37.0
76-77	36.32375	37.0	37.0	37.0	37.0	37.0
78-79	36.321	37.0	37.0	37.0	37.0	37.0
80-81	36.28625	37.0	37.0	37.0	37.0	37.0
82-83	36.26025	37.0	37.0	37.0	37.0	37.0
84-85	36.26225	37.0	37.0	37.0	37.0	37.0
86-87	36.23375	37.0	37.0	37.0	37.0	37.0
88-89	36.20225000000001	37.0	37.0	37.0	37.0	37.0
90-91	36.12925	37.0	37.0	37.0	37.0	37.0
92-93	36.167500000000004	37.0	37.0	37.0	37.0	37.0
94-95	36.21	37.0	37.0	37.0	37.0	37.0
96-97	36.164249999999996	37.0	37.0	37.0	37.0	37.0
98-99	36.114125	37.0	37.0	37.0	37.0	37.0
100-101	35.982749999999996	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	1.0
27	8.0
28	13.0
29	14.0
30	14.0
31	24.0
32	31.0
33	51.0
34	85.0
35	268.0
36	2657.0
37	834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.975	10.75	12.775	41.5
2	17.779444861215303	15.128782195548887	37.43435858964741	29.657414353588397
3	18.084042021010504	19.18459229614807	32.666333166583286	30.06503251625813
4	24.55	24.775	24.4	26.275
5	24.55	31.474999999999998	22.425	21.55
6	23.175	31.4	28.925	16.5
7	17.0	24.025	43.824999999999996	15.15
8	21.175	21.9	31.225	25.7
9	17.275	25.15	35.875	21.7
10-11	21.725	33.0125	24.8625	20.4
12-13	22.162499999999998	24.275	29.912499999999998	23.65
14-15	20.775	28.475	29.1375	21.6125
16-17	19.35	27.8875	28.325	24.4375
18-19	22.9625	29.299999999999997	26.387500000000003	21.349999999999998
20-21	22.8125	26.7625	27.55	22.875
22-23	22.725	26.3625	26.35	24.5625
24-25	23.5875	28.65	26.8625	20.9
26-27	22.0125	29.1625	26.775	22.05
28-29	20.1375	27.987499999999997	29.562500000000004	22.3125
30-31	21.675	26.737499999999997	28.812500000000004	22.775000000000002
32-33	21.525	26.775	29.2875	22.412499999999998
34-35	20.7125	26.2625	27.5125	25.5125
36-37	22.0	28.025	29.3875	20.5875
38-39	21.025	28.849999999999998	29.7	20.424999999999997
40-41	22.5	28.3375	29.2375	19.925
42-43	23.3375	31.275	25.6125	19.775000000000002
44-45	20.3875	30.65	25.7875	23.175
46-47	24.825	30.5125	23.375	21.2875
48-49	21.4	30.8125	25.7625	22.025
50-51	23.65	26.8125	27.675	21.8625
52-53	22.775000000000002	26.5625	28.537499999999998	22.125
54-55	21.6875	25.575	29.462500000000002	23.275000000000002
56-57	19.675	29.762499999999996	27.2625	23.3
58-59	21.7375	26.2125	28.212500000000002	23.8375
60-61	20.7	30.0375	26.3	22.9625
62-63	24.4125	27.85	26.724999999999998	21.0125
64-65	23.325000000000003	26.224999999999998	27.500000000000004	22.95
66-67	25.05	26.5125	27.025	21.4125
68-69	22.3	27.3875	26.637499999999996	23.674999999999997
70-71	22.8625	28.4125	26.5625	22.162499999999998
72-73	21.5375	30.1875	26.5875	21.6875
74-75	21.775	28.050000000000004	27.650000000000002	22.525000000000002
76-77	25.775	26.875	25.8125	21.5375
78-79	24.0625	26.575	27.150000000000002	22.2125
80-81	23.825	26.6	27.0875	22.4875
82-83	24.7	28.1875	26.674999999999997	20.4375
84-85	21.087500000000002	29.9875	24.3625	24.5625
86-87	22.875	28.475	25.025	23.625
88-89	24.8	26.650000000000002	27.8125	20.7375
90-91	22.8625	26.8125	27.650000000000002	22.675
92-93	23.200000000000003	26.0	27.8625	22.9375
94-95	21.8125	29.4375	26.5	22.25
96-97	21.45	27.375	24.762500000000003	26.4125
98-99	21.29016127015877	28.141017627203404	29.678709838729837	20.890111263907986
100-101	22.475	27.1625	27.450000000000003	22.912499999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	2.0
22	3.5
23	5.0
24	2.5
25	9.0
26	18.5
27	11.0
28	7.5
29	13.5
30	28.5
31	53.0
32	103.5
33	141.5
34	132.0
35	141.0
36	118.5
37	83.5
38	69.5
39	54.0
40	80.5
41	150.5
42	194.0
43	164.0
44	148.0
45	164.0
46	235.0
47	287.5
48	224.0
49	166.0
50	147.0
51	122.5
52	103.5
53	86.5
54	87.0
55	86.0
56	70.5
57	58.0
58	70.0
59	102.0
60	93.5
61	54.5
62	37.0
63	25.5
64	9.5
65	4.5
66	0.5
67	2.0
68	2.5
69	1.5
70	3.5
71	4.5
72	3.0
73	2.0
74	1.0
75	0.5
76	3.0
77	2.5
78	1.5
79	1.5
80	0.0
81	0.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.05
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0125
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.2459797498511	22.35
2	21.858248957712924	18.35
3	10.363311494937463	13.05
4	4.228707564026206	7.1
5	2.858844550327576	6.0
6	1.3103037522334724	3.3000000000000003
7	1.429422275163788	4.2
8	0.6551518761167362	2.1999999999999997
9	0.714711137581894	2.7
>10	3.275759380583681	19.45
>50	0.05955926146515784	1.3
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	52	1.3	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	42	1.05	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	27	0.675	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	27	0.675	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	22	0.5499999999999999	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	22	0.5499999999999999	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	19	0.475	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	17	0.42500000000000004	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	17	0.42500000000000004	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	17	0.42500000000000004	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	16	0.4	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	16	0.4	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	16	0.4	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	15	0.375	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	15	0.375	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	15	0.375	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	14	0.35000000000000003	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	14	0.35000000000000003	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	14	0.35000000000000003	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	14	0.35000000000000003	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	13	0.325	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	13	0.325	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	13	0.325	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	13	0.325	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	13	0.325	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	13	0.325	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	13	0.325	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	13	0.325	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	13	0.325	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	13	0.325	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	12	0.3	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	12	0.3	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	12	0.3	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	12	0.3	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	12	0.3	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	12	0.3	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	12	0.3	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	12	0.3	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	12	0.3	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	12	0.3	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	12	0.3	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	11	0.27499999999999997	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	11	0.27499999999999997	No Hit
CTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAA	11	0.27499999999999997	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	11	0.27499999999999997	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	11	0.27499999999999997	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	11	0.27499999999999997	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	11	0.27499999999999997	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	10	0.25	No Hit
CTTGTCATAAAGCTCTTCCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGG	10	0.25	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	10	0.25	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	10	0.25	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	10	0.25	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	10	0.25	No Hit
CCACAATGAGGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGA	10	0.25	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	10	0.25	No Hit
GTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATG	9	0.22499999999999998	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	9	0.22499999999999998	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	9	0.22499999999999998	No Hit
GGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTA	9	0.22499999999999998	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	9	0.22499999999999998	No Hit
CGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGG	9	0.22499999999999998	No Hit
TACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAA	9	0.22499999999999998	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	9	0.22499999999999998	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	9	0.22499999999999998	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	9	0.22499999999999998	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	9	0.22499999999999998	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	9	0.22499999999999998	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	8	0.2	No Hit
CACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTAT	8	0.2	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	8	0.2	No Hit
TGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCT	8	0.2	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	8	0.2	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	8	0.2	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	8	0.2	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	8	0.2	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	8	0.2	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	8	0.2	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	8	0.2	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	7	0.17500000000000002	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	7	0.17500000000000002	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	7	0.17500000000000002	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	7	0.17500000000000002	No Hit
GGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGT	7	0.17500000000000002	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	7	0.17500000000000002	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	7	0.17500000000000002	No Hit
ATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTCA	7	0.17500000000000002	No Hit
CGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTAC	7	0.17500000000000002	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	7	0.17500000000000002	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	7	0.17500000000000002	No Hit
GGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGG	7	0.17500000000000002	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	7	0.17500000000000002	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	7	0.17500000000000002	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	7	0.17500000000000002	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	7	0.17500000000000002	No Hit
AGGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCA	7	0.17500000000000002	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	7	0.17500000000000002	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	7	0.17500000000000002	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	7	0.17500000000000002	No Hit
GTGGGTCATGCTCCTGTGAGTCCCAAAGGGGTGCATGCTTGGCCAGTGTT	7	0.17500000000000002	No Hit
AAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACAC	7	0.17500000000000002	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	7	0.17500000000000002	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	7	0.17500000000000002	No Hit
CTGATTTGTTATTTTTGATGCTTTAGTAGATGTCACCAATGAACTATGGG	6	0.15	No Hit
GGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAG	6	0.15	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	6	0.15	No Hit
ACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGC	6	0.15	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	6	0.15	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	6	0.15	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	6	0.15	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	6	0.15	No Hit
GTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCGGAAGGTCTTGTTTGGG	6	0.15	No Hit
TTTTTAAGAAAAGTATCTGTGGGGTCCAAATCCCAGGTTGCTTTCTTCAT	6	0.15	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	6	0.15	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	6	0.15	No Hit
GTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAA	6	0.15	No Hit
AACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACA	6	0.15	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	6	0.15	No Hit
GCTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAA	6	0.15	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	6	0.15	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	6	0.15	No Hit
CCCGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	6	0.15	No Hit
CGCCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	6	0.15	No Hit
CCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGTTGGAGAACGCAAAT	6	0.15	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	6	0.15	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	5	0.125	No Hit
GCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTCACGGCAGG	5	0.125	No Hit
CCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATG	5	0.125	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	5	0.125	No Hit
CAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATG	5	0.125	No Hit
AGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTC	5	0.125	No Hit
CTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGG	5	0.125	No Hit
GCCAGTGTGGTGCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAG	5	0.125	No Hit
ACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACTG	5	0.125	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	5	0.125	No Hit
CCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGT	5	0.125	No Hit
CAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG	5	0.125	No Hit
CTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCT	5	0.125	No Hit
GGGTCATGCTCCTGTGAGTCCCAAAGGGGTGCATGCTTGGCCAGTGTTCT	5	0.125	No Hit
ATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCT	5	0.125	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	5	0.125	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	5	0.125	No Hit
CTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAG	5	0.125	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	5	0.125	No Hit
CTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGTA	5	0.125	No Hit
TTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAAT	5	0.125	No Hit
CTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAG	5	0.125	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	5	0.125	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	5	0.125	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	5	0.125	No Hit
CTCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCCA	5	0.125	No Hit
GTTCCGGGATCACCTTCTTTTGCAGAACTTTCCACACCCATCACCAAAAT	5	0.125	No Hit
TGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACAT	5	0.125	No Hit
GTCGTCTGGTGTGGTGCTGGGCGTGTACCAGTAGCTTAAAGGCTCTCCAT	5	0.125	No Hit
GAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAAATTAAATA	5	0.125	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	5	0.125	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	5	0.125	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	5	0.125	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	5	0.125	No Hit
GCTCCTTACGGTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGT	5	0.125	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	5	0.125	No Hit
TTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTT	5	0.125	No Hit
TGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTT	5	0.125	No Hit
TTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCAT	5	0.125	No Hit
AATAAAATTTATGAATTAAGCCCTTGACCAACTCAACACATATGCAGTAT	5	0.125	No Hit
GTTTTATTTTGAGACAGTTCTGCTGTGTTATTGTTGGCCTCAAACTGGGC	5	0.125	No Hit
AAACCCTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTAC	5	0.125	No Hit
TGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCT	5	0.125	No Hit
CTGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTA	5	0.125	No Hit
GTAATTTCGAAGCTCTTTCCTCCTCGCTGTTGGCATCAAGAAGATCATCA	5	0.125	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	5	0.125	No Hit
TTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.05	0.0	0.0	0.0	0.0
76-77	0.05	0.0	0.0	0.0	0.0
78-79	0.1	0.0	0.0	0.0	0.0
80-81	0.15	0.0	0.0	0.0	0.0
82-83	0.21250000000000002	0.0	0.0	0.0	0.0
84-85	0.2875	0.0	0.0	0.0	0.0
86-87	0.35	0.0	0.0	0.0	0.0
88-89	0.4625	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882426 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882426_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3435	37.0	37.0	37.0	37.0	37.0
2	36.3295	37.0	37.0	37.0	37.0	37.0
3	36.4155	37.0	37.0	37.0	37.0	37.0
4	36.4075	37.0	37.0	37.0	37.0	37.0
5	36.4365	37.0	37.0	37.0	37.0	37.0
6	36.3495	37.0	37.0	37.0	37.0	37.0
7	36.411	37.0	37.0	37.0	37.0	37.0
8	36.521	37.0	37.0	37.0	37.0	37.0
9	36.5015	37.0	37.0	37.0	37.0	37.0
10-11	36.429249999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.46875	37.0	37.0	37.0	37.0	37.0
14-15	36.411500000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.437	37.0	37.0	37.0	37.0	37.0
18-19	36.379000000000005	37.0	37.0	37.0	37.0	37.0
20-21	36.33575	37.0	37.0	37.0	37.0	37.0
22-23	36.37	37.0	37.0	37.0	37.0	37.0
24-25	36.37975	37.0	37.0	37.0	37.0	37.0
26-27	36.34	37.0	37.0	37.0	37.0	37.0
28-29	36.381249999999994	37.0	37.0	37.0	37.0	37.0
30-31	36.33825	37.0	37.0	37.0	37.0	37.0
32-33	36.3765	37.0	37.0	37.0	37.0	37.0
34-35	36.3075	37.0	37.0	37.0	37.0	37.0
36-37	36.335	37.0	37.0	37.0	37.0	37.0
38-39	36.371	37.0	37.0	37.0	37.0	37.0
40-41	36.325500000000005	37.0	37.0	37.0	37.0	37.0
42-43	36.218	37.0	37.0	37.0	37.0	37.0
44-45	36.19	37.0	37.0	37.0	37.0	37.0
46-47	36.19175	37.0	37.0	37.0	37.0	37.0
48-49	36.08125	37.0	37.0	37.0	37.0	37.0
50-51	36.1525	37.0	37.0	37.0	37.0	37.0
52-53	36.1185	37.0	37.0	37.0	37.0	37.0
54-55	36.069	37.0	37.0	37.0	37.0	37.0
56-57	36.0875	37.0	37.0	37.0	37.0	37.0
58-59	36.151125	37.0	37.0	37.0	37.0	37.0
60-61	36.064750000000004	37.0	37.0	37.0	37.0	37.0
62-63	36.008250000000004	37.0	37.0	37.0	37.0	37.0
64-65	36.0305	37.0	37.0	37.0	37.0	37.0
66-67	36.102000000000004	37.0	37.0	37.0	37.0	37.0
68-69	35.957499999999996	37.0	37.0	37.0	37.0	37.0
70-71	35.928	37.0	37.0	37.0	37.0	37.0
72-73	35.90525	37.0	37.0	37.0	37.0	37.0
74-75	35.974000000000004	37.0	37.0	37.0	37.0	37.0
76-77	35.9105	37.0	37.0	37.0	37.0	37.0
78-79	35.98375	37.0	37.0	37.0	37.0	37.0
80-81	35.95	37.0	37.0	37.0	37.0	37.0
82-83	35.883250000000004	37.0	37.0	37.0	37.0	37.0
84-85	35.8535	37.0	37.0	37.0	37.0	37.0
86-87	35.8485	37.0	37.0	37.0	37.0	37.0
88-89	35.7815	37.0	37.0	37.0	37.0	37.0
90-91	35.800749999999994	37.0	37.0	37.0	37.0	37.0
92-93	35.779125	37.0	37.0	37.0	37.0	37.0
94-95	35.80525	37.0	37.0	37.0	37.0	37.0
96-97	35.74825	37.0	37.0	37.0	37.0	37.0
98-99	35.7395	37.0	37.0	37.0	37.0	37.0
100-101	35.44125	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	1.0
23	1.0
24	0.0
25	5.0
26	3.0
27	10.0
28	13.0
29	14.0
30	23.0
31	31.0
32	40.0
33	68.0
34	174.0
35	502.0
36	2728.0
37	386.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.88482724086129	18.42764146219329	14.321482223335003	27.366049073610416
2	18.8	27.800000000000004	36.075	17.325
3	21.45	27.625	31.574999999999996	19.35
4	27.525	32.550000000000004	20.025000000000002	19.900000000000002
5	25.124999999999996	35.0	24.275	15.6
6	22.400000000000002	35.3	23.05	19.25
7	20.075000000000003	21.4	39.225	19.3
8	23.275000000000002	21.9	29.4	25.424999999999997
9	20.75	25.25	30.675	23.325000000000003
10-11	23.8875	32.75	22.7375	20.625
12-13	24.3	26.450000000000003	26.5875	22.662499999999998
14-15	24.0125	26.174999999999997	28.799999999999997	21.0125
16-17	23.5	28.675	28.0625	19.7625
18-19	22.4625	29.225	27.725	20.5875
20-21	23.0375	29.099999999999998	28.225	19.6375
22-23	24.45	28.275	25.775	21.5
24-25	22.9375	28.4375	27.125	21.5
26-27	23.0125	27.650000000000002	28.025	21.3125
28-29	23.2375	27.224999999999998	28.1625	21.375
30-31	23.962500000000002	26.8375	29.049999999999997	20.150000000000002
32-33	22.662499999999998	26.625	28.9125	21.8
34-35	23.2125	27.6625	27.6	21.525
36-37	23.8125	28.849999999999998	27.6125	19.725
38-39	22.5125	27.800000000000004	27.125	22.5625
40-41	22.55	27.525	28.3375	21.587500000000002
42-43	22.55	29.575000000000003	26.937499999999996	20.9375
44-45	23.075000000000003	27.474999999999998	30.0375	19.412499999999998
46-47	23.7125	27.5625	26.025	22.7
48-49	22.3375	27.425	29.0875	21.15
50-51	22.05	28.225	26.05	23.674999999999997
52-53	23.962500000000002	27.650000000000002	29.3875	19.0
54-55	25.7625	27.175	25.7875	21.275
56-57	21.7375	27.275	29.0875	21.9
58-59	20.80260032504063	28.62857857232154	28.078509813726715	22.490311288911112
60-61	20.7875	29.625	28.025	21.5625
62-63	22.7375	29.275000000000002	26.5	21.4875
64-65	23.2375	28.449999999999996	29.062500000000004	19.25
66-67	22.2125	27.525	27.275	22.9875
68-69	23.25	27.625	28.549999999999997	20.575
70-71	21.587500000000002	28.1125	29.4375	20.8625
72-73	23.5875	27.950000000000003	27.250000000000004	21.212500000000002
74-75	24.65	26.187500000000004	28.449999999999996	20.7125
76-77	22.6125	28.037499999999998	27.900000000000002	21.45
78-79	23.849999999999998	27.1375	28.812500000000004	20.200000000000003
80-81	23.674999999999997	25.912499999999998	29.299999999999997	21.1125
82-83	22.975	26.650000000000002	29.037499999999998	21.337500000000002
84-85	21.05	27.5625	29.812499999999996	21.575
86-87	22.05	28.8625	27.3625	21.725
88-89	22.75	27.750000000000004	28.65	20.849999999999998
90-91	23.75	27.0625	27.5875	21.6
92-93	23.71546443305413	29.566195774471808	26.740842605325664	19.977497187148394
94-95	22.0	30.2125	25.45	22.3375
96-97	23.4375	26.900000000000002	27.212500000000002	22.45
98-99	23.8625	28.962500000000002	26.4625	20.7125
100-101	23.6375	28.9375	26.487500000000004	20.9375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	1.0
24	1.0
25	1.0
26	2.5
27	2.0
28	4.0
29	18.5
30	46.0
31	109.0
32	146.5
33	116.0
34	101.0
35	98.0
36	96.0
37	108.0
38	111.5
39	98.5
40	109.0
41	164.0
42	207.5
43	198.5
44	207.0
45	226.0
46	229.5
47	248.0
48	205.0
49	145.0
50	128.5
51	114.0
52	99.0
53	79.5
54	76.5
55	102.0
56	76.5
57	31.0
58	60.0
59	68.0
60	31.0
61	16.0
62	10.5
63	8.0
64	8.0
65	5.5
66	1.5
67	1.5
68	5.5
69	5.0
70	1.0
71	4.0
72	7.0
73	7.5
74	8.5
75	9.5
76	7.0
77	12.0
78	12.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0125
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.09115870400879	24.625
2	21.85612300933553	19.900000000000002
3	8.896210873146623	12.15
4	5.601317957166392	10.2
5	3.2399780340472266	7.375
6	1.9220208676551345	5.25
7	1.043382756727073	3.325
8	0.9335529928610654	3.4000000000000004
9	0.6040637012630422	2.475
>10	1.8121911037891267	11.3
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	28	0.7000000000000001	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	27	0.675	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	23	0.575	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	18	0.44999999999999996	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	18	0.44999999999999996	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	17	0.42500000000000004	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	15	0.375	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	15	0.375	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	15	0.375	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	14	0.35000000000000003	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	14	0.35000000000000003	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	14	0.35000000000000003	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	13	0.325	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	13	0.325	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	12	0.3	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	12	0.3	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	12	0.3	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	12	0.3	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	12	0.3	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	11	0.27499999999999997	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	11	0.27499999999999997	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	11	0.27499999999999997	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	11	0.27499999999999997	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	11	0.27499999999999997	No Hit
TATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCT	11	0.27499999999999997	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	11	0.27499999999999997	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	11	0.27499999999999997	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	10	0.25	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	10	0.25	No Hit
GTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTG	10	0.25	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	10	0.25	No Hit
GCAGGTCAAAAGGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATT	10	0.25	No Hit
CCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAAT	10	0.25	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	9	0.22499999999999998	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	9	0.22499999999999998	No Hit
CCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTA	9	0.22499999999999998	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	9	0.22499999999999998	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	9	0.22499999999999998	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	9	0.22499999999999998	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	9	0.22499999999999998	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	9	0.22499999999999998	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	9	0.22499999999999998	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	9	0.22499999999999998	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	9	0.22499999999999998	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	8	0.2	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	8	0.2	No Hit
GCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGT	8	0.2	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	8	0.2	No Hit
CTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCAT	8	0.2	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	8	0.2	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	8	0.2	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	8	0.2	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	8	0.2	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	8	0.2	No Hit
GGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTG	8	0.2	No Hit
CCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGGTGCTTTGAACA	8	0.2	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	8	0.2	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	8	0.2	No Hit
AAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAG	8	0.2	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	8	0.2	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	8	0.2	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	7	0.17500000000000002	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	7	0.17500000000000002	No Hit
CTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATT	7	0.17500000000000002	No Hit
GAAGAACTCAACTACATAAAGACAGAGGGACAATCAAAACTGCACAGAGG	7	0.17500000000000002	No Hit
ATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACA	7	0.17500000000000002	No Hit
GTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACT	7	0.17500000000000002	No Hit
TGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTG	7	0.17500000000000002	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	7	0.17500000000000002	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	7	0.17500000000000002	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	7	0.17500000000000002	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	7	0.17500000000000002	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	7	0.17500000000000002	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	7	0.17500000000000002	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	7	0.17500000000000002	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	7	0.17500000000000002	No Hit
TTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTG	7	0.17500000000000002	No Hit
CTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCA	7	0.17500000000000002	No Hit
GTCGGTGGTGACTGGCGGCGGTGAGGCGGCCGGTGGCGGCGGCGGCGGCG	7	0.17500000000000002	No Hit
CAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATG	7	0.17500000000000002	No Hit
CCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTG	6	0.15	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	6	0.15	No Hit
CTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGA	6	0.15	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	6	0.15	No Hit
CCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTT	6	0.15	No Hit
GGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTC	6	0.15	No Hit
CTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAG	6	0.15	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	6	0.15	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	6	0.15	No Hit
CAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATT	6	0.15	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	6	0.15	No Hit
GAAAAAACTATGAAGCACGTGATGCAAGTTCTAGAGAGACACGAAACCCA	6	0.15	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	6	0.15	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	6	0.15	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	6	0.15	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	6	0.15	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	6	0.15	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	6	0.15	No Hit
GGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAG	6	0.15	No Hit
CGGCAAAGAACACTGGCCAAGCATGCACCCCTTTGGGACTCACAGGAGCA	6	0.15	No Hit
CCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAG	6	0.15	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	6	0.15	No Hit
CAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAG	6	0.15	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	6	0.15	No Hit
GGGAGGACTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAA	6	0.15	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	6	0.15	No Hit
CCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCT	6	0.15	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	6	0.15	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	6	0.15	No Hit
AGCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGA	6	0.15	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	6	0.15	No Hit
GTGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAG	6	0.15	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	6	0.15	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	6	0.15	No Hit
AGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAA	6	0.15	No Hit
GTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACA	5	0.125	No Hit
AATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCA	5	0.125	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	5	0.125	No Hit
GACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCT	5	0.125	No Hit
CTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGA	5	0.125	No Hit
CGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGT	5	0.125	No Hit
GTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTT	5	0.125	No Hit
CAGACCTAATGCGGAATCACCTCAATGAGAAGCGAGCACTCCGCTTGGAG	5	0.125	No Hit
TTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGC	5	0.125	No Hit
GTCTGTCTCAAGAGCTGGTCTCCTGTGGCTATGTGGAAGTGACAAGCCTG	5	0.125	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	5	0.125	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	5	0.125	No Hit
CCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGAT	5	0.125	No Hit
ATGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACA	5	0.125	No Hit
ATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCT	5	0.125	No Hit
ATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCAA	5	0.125	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	5	0.125	No Hit
ATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCC	5	0.125	No Hit
GAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCTC	5	0.125	No Hit
GCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCC	5	0.125	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	5	0.125	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	5	0.125	No Hit
CCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCA	5	0.125	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	5	0.125	No Hit
GAAAAAAAGAATATCAACTGCAGTGAACTCCATGAGCACATAAACACACA	5	0.125	No Hit
CTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGT	5	0.125	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	5	0.125	No Hit
GTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGG	5	0.125	No Hit
TAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCAAA	5	0.125	No Hit
GACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGA	5	0.125	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
GACATGTCGGTGGTGACTGGCGGCGGTGAGGCGGCCGGTGGCGGCGGCGG	5	0.125	No Hit
ACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAA	5	0.125	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	5	0.125	No Hit
AACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTT	5	0.125	No Hit
GAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGC	5	0.125	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	5	0.125	No Hit
GACGCCACCAGCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTT	5	0.125	No Hit
TATGAAGTGGCTCTCGTTCACTGGGAGAACGAAGAACTCAACTACATAAA	5	0.125	No Hit
CAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAA	5	0.125	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	5	0.125	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	5	0.125	No Hit
AAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAA	5	0.125	No Hit
TCCAGGACCAACCTTCCAGATCTGTCTGTGAATGAGGACCTGATGCAGTG	5	0.125	No Hit
CATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATG	5	0.125	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	5	0.125	No Hit
TTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAG	5	0.125	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	5	0.125	No Hit
TGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTC	5	0.125	No Hit
GTCAAACTGTCTCATAAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGC	5	0.125	No Hit
CTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAA	5	0.125	No Hit
TTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACAC	5	0.125	No Hit
ATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTC	5	0.125	No Hit
TTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTTT	5	0.125	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	5	0.125	No Hit
TCGGTGGTGACTGGCGGCGGTGAGGCGGCCGGTGGCGGCGGCGGCGGCGG	5	0.125	No Hit
AAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGC	5	0.125	No Hit
TAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAA	5	0.125	No Hit
GGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.0625	0.0	0.0	0.0	0.0
74-75	0.075	0.0	0.0	0.0	0.0
76-77	0.075	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.175	0.0	0.0	0.0	0.0
82-83	0.225	0.0	0.0	0.0	0.0
84-85	0.2875	0.0	0.0	0.0	0.0
86-87	0.35	0.0	0.0	0.0	0.0
88-89	0.44999999999999996	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
Read 4644976 spots for SRR24882426.sra
Written 4644976 spots for SRR24882426.sra
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
Read 4644971 spots for SRR24882426.sra
Written 4644971 spots for SRR24882426.sra
SRR ids: ['SRR24882426.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6iy2vjzh
SRR24882426.sra spots: 37159773
blocks: [[1, 4644971], [4644972, 9289942], [9289943, 13934913], [13934914, 18579884], [18579885, 23224855], [23224856, 27869826], [27869827, 32514797], [32514798, 37159773]]
SRR24882426 file size 8977940
SRR24882426 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882426 SRR24882426_1.fastq SRR24882426_2.fastq
Input file:	SRR24882426_1.fastq
Paired file:	SRR24882426_2.fastq
trimmed:	SRR24882426-trimmed-pair1.fastq, SRR24882426-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 03:00:42 2026 >> started

Fri Jul  3 03:01:22 2026 >> done (39.272s)
37159773 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
37159773 (100.00%) read pairs available; of these:
  605598 ( 1.63%) trimmed read pairs available after processing
36554175 (98.37%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 64	       2	  0.00%
 65	       1	  0.00%
 66	      25	  0.00%
 67	    1005	  0.00%
 68	    4700	  0.01%
 69	    5630	  0.02%
 70	    6645	  0.02%
 71	    7127	  0.02%
 72	    7952	  0.02%
 73	    8515	  0.02%
 74	    8855	  0.02%
 75	    9849	  0.03%
 76	   10304	  0.03%
 77	   11139	  0.03%
 78	   11740	  0.03%
 79	   12446	  0.03%
 80	   12977	  0.03%
 81	   13593	  0.04%
 82	   14758	  0.04%
 83	   15630	  0.04%
 84	   16264	  0.04%
 85	   17376	  0.05%
 86	   17504	  0.05%
 87	   18378	  0.05%
 88	   20055	  0.05%
 89	   19971	  0.05%
 90	   21831	  0.06%
 91	   23523	  0.06%
 92	   23738	  0.06%
 93	   24670	  0.07%
 94	   25886	  0.07%
 95	   26895	  0.07%
 96	   28072	  0.08%
 97	   29845	  0.08%
 98	   30508	  0.08%
 99	   33322	  0.09%
100	   64867	  0.17%
101	36554175	 98.37%
37159773 reads passed initial QC


criterion=sequence-density
sequence-density=0.58
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=29
prefix-density=0.57
prefix-fanout=2.0
sequence=GATGGGAACAGGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=33.37
fanout-score-rank=1
prefix-density=0.15
prefix-fanout=4.2
sequence=CAAAGATCTTCACATTTTGCTCATCTCTTTGGTCACTTTCCTGCACTTGGATCAATCACTCTTCGCTTATGATTTTGGTGTGAACCTCCCGGCTCTTCACCCGCAGCTTGTTGACCTGGGACTCCGCGATGTCAGCCCGCTCCTCGGCTTCCTCCAGCTCGTGCTGGATCTTGCGGAA


criterion=sequence-density
sequence-density=0.34
sequence-density-rank=1
fanout-score=4.22
fanout-score-rank=21
prefix-density=0.45
prefix-fanout=3.2
sequence=ATGAGGAGATCG


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=32
fanout-score=55.51
fanout-score-rank=1
prefix-density=0.73
prefix-fanout=4.9
sequence=AAGAAGCTGGCGGAGCCACTTCAGCCCAAATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCA
SRR24882426 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 03:01:37
                             Started mapping on |	Jul 03 03:01:37
                                    Finished on |	Jul 03 03:19:06
       Mapping speed, Million of reads per hour |	127.53

                          Number of input reads |	37159773
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	33480133
                        Uniquely mapped reads % |	90.10%
                          Average mapped length |	200.58
                       Number of splices: Total |	25341695
            Number of splices: Annotated (sjdb) |	25166789
                       Number of splices: GT/AG |	25166787
                       Number of splices: GC/AG |	140159
                       Number of splices: AT/AC |	13676
               Number of splices: Non-canonical |	21073
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.89
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.77
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3671552
             % of reads mapped to multiple loci |	9.88%
        Number of reads mapped to too many loci |	3098
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8088	8088	8088
N_multimapping	3671552	3671552	3671552
N_noFeature	2281487	29210682	3298658
N_ambiguous	3549636	1319951	289903
UnstrandedReadsAssigned:27649010 PositiveStrandReadsAssigned:2949500 NegativeStrandReadsAssigned:29891572
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882426 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882426-trimmed-pair1.fastq
                             SRR24882426-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 37,159,773 reads, 31,003,963 reads pseudoaligned
[quant] estimated average fragment length: 224.03
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,349 rounds

 109283 SRR24882426.ke.tsv
  52636 SRR24882426.se.tsv
 161919 total
==> SRR24882426.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9	0	0
ENSMUST00000196221.1	9	2	0	0
ENSMUST00000179664.1	11	4	0	0
ENSMUST00000178537.1	12	5	0	0
ENSMUST00000178862.1	14	7	0	0
ENSMUST00000179520.1	11	4	0	0
ENSMUST00000179883.1	16	9	0	0
ENSMUST00000195858.1	10	3	0	0
ENSMUST00000179932.1	12	5	0	0

==> SRR24882426.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882426 completed mapping pipeline successfully
