Starting /dee2/code/volunteer_pipeline.sh SRR24882428
    current disk space = 1342679664
    free memory = 198172448 
SRR24882428 SRAfilesize
021fb485d650be507e032c89e769c788  SRR24882428.sra
SRR24882428.sra file validated
SRR24882428 is paired end
SRR24882428 is conventional basespace
SRR24882428 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.598	37.0	37.0	37.0	37.0	37.0
2	36.58775	37.0	37.0	37.0	37.0	37.0
3	36.59025	37.0	37.0	37.0	37.0	37.0
4	36.5925	37.0	37.0	37.0	37.0	37.0
5	36.641	37.0	37.0	37.0	37.0	37.0
6	36.615	37.0	37.0	37.0	37.0	37.0
7	36.538	37.0	37.0	37.0	37.0	37.0
8	36.537	37.0	37.0	37.0	37.0	37.0
9	36.6015	37.0	37.0	37.0	37.0	37.0
10-11	36.63375	37.0	37.0	37.0	37.0	37.0
12-13	36.633250000000004	37.0	37.0	37.0	37.0	37.0
14-15	36.585750000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.67275	37.0	37.0	37.0	37.0	37.0
18-19	36.593	37.0	37.0	37.0	37.0	37.0
20-21	36.61525	37.0	37.0	37.0	37.0	37.0
22-23	36.613749999999996	37.0	37.0	37.0	37.0	37.0
24-25	36.6265	37.0	37.0	37.0	37.0	37.0
26-27	36.5355	37.0	37.0	37.0	37.0	37.0
28-29	36.57125	37.0	37.0	37.0	37.0	37.0
30-31	36.51575	37.0	37.0	37.0	37.0	37.0
32-33	36.54225	37.0	37.0	37.0	37.0	37.0
34-35	36.5355	37.0	37.0	37.0	37.0	37.0
36-37	36.506249999999994	37.0	37.0	37.0	37.0	37.0
38-39	36.503249999999994	37.0	37.0	37.0	37.0	37.0
40-41	36.5065	37.0	37.0	37.0	37.0	37.0
42-43	36.526250000000005	37.0	37.0	37.0	37.0	37.0
44-45	36.48625	37.0	37.0	37.0	37.0	37.0
46-47	36.505	37.0	37.0	37.0	37.0	37.0
48-49	36.375	37.0	37.0	37.0	37.0	37.0
50-51	36.5005	37.0	37.0	37.0	37.0	37.0
52-53	36.4735	37.0	37.0	37.0	37.0	37.0
54-55	36.403	37.0	37.0	37.0	37.0	37.0
56-57	36.44925	37.0	37.0	37.0	37.0	37.0
58-59	36.4585	37.0	37.0	37.0	37.0	37.0
60-61	36.4045	37.0	37.0	37.0	37.0	37.0
62-63	36.442750000000004	37.0	37.0	37.0	37.0	37.0
64-65	36.404250000000005	37.0	37.0	37.0	37.0	37.0
66-67	36.38475	37.0	37.0	37.0	37.0	37.0
68-69	36.30825	37.0	37.0	37.0	37.0	37.0
70-71	36.35925	37.0	37.0	37.0	37.0	37.0
72-73	36.333749999999995	37.0	37.0	37.0	37.0	37.0
74-75	36.2555	37.0	37.0	37.0	37.0	37.0
76-77	36.35525	37.0	37.0	37.0	37.0	37.0
78-79	36.33775	37.0	37.0	37.0	37.0	37.0
80-81	36.34125	37.0	37.0	37.0	37.0	37.0
82-83	36.312250000000006	37.0	37.0	37.0	37.0	37.0
84-85	36.37475	37.0	37.0	37.0	37.0	37.0
86-87	36.286	37.0	37.0	37.0	37.0	37.0
88-89	36.301	37.0	37.0	37.0	37.0	37.0
90-91	36.2475	37.0	37.0	37.0	37.0	37.0
92-93	36.28075	37.0	37.0	37.0	37.0	37.0
94-95	36.22175	37.0	37.0	37.0	37.0	37.0
96-97	36.161249999999995	37.0	37.0	37.0	37.0	37.0
98-99	36.132999999999996	37.0	37.0	37.0	37.0	37.0
100-101	36.036500000000004	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	1.0
24	0.0
25	2.0
26	2.0
27	3.0
28	3.0
29	19.0
30	20.0
31	21.0
32	29.0
33	57.0
34	74.0
35	233.0
36	2640.0
37	895.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.5	9.375	14.2	38.925
2	17.663247435576682	14.635976982737054	38.10357768326244	29.597197898423815
3	18.413810357768327	18.764073054791094	31.148361270953217	31.673755316487366
4	24.425	27.725	22.925	24.925
5	24.025	30.175	24.85	20.95
6	19.875	31.45	29.875	18.8
7	16.775000000000002	23.799999999999997	42.75	16.675
8	21.925	21.475	31.775	24.825
9	19.825	22.825	33.525	23.825
10-11	22.1375	33.0875	23.4125	21.3625
12-13	21.55	24.125	28.762500000000003	25.5625
14-15	20.1875	27.6625	29.362500000000004	22.787499999999998
16-17	19.475	28.325	27.3625	24.837500000000002
18-19	23.3125	28.0875	26.025	22.575
20-21	22.025	27.125	28.4375	22.412499999999998
22-23	21.775	25.974999999999998	27.462500000000002	24.7875
24-25	23.65	27.200000000000003	26.724999999999998	22.425
26-27	21.462500000000002	28.3875	27.787499999999998	22.3625
28-29	19.5625	27.825	29.975	22.6375
30-31	22.287499999999998	26.437500000000004	28.1375	23.1375
32-33	22.112499999999997	24.925	29.65	23.3125
34-35	19.325	26.5	28.775000000000002	25.4
36-37	22.037499999999998	27.1625	29.862499999999997	20.9375
38-39	22.025	26.9125	29.9875	21.075
40-41	20.974999999999998	28.512500000000003	29.425	21.087500000000002
42-43	21.4125	30.3875	25.825	22.375
44-45	19.875	29.2375	26.937499999999996	23.95
46-47	23.150000000000002	29.1875	24.4875	23.175
48-49	21.825	29.625	25.75	22.8
50-51	22.475	27.0875	27.1625	23.275000000000002
52-53	22.162499999999998	25.7375	27.950000000000003	24.15
54-55	21.087500000000002	25.837500000000002	28.962500000000002	24.1125
56-57	20.5125	28.462500000000002	28.037499999999998	22.9875
58-59	22.1375	25.25	29.762499999999996	22.85
60-61	20.575	28.725	27.762500000000003	22.9375
62-63	23.425	25.937500000000004	29.099999999999998	21.5375
64-65	22.537499999999998	24.887500000000003	28.762500000000003	23.8125
66-67	23.4625	25.0625	28.762500000000003	22.7125
68-69	22.0	26.5625	27.474999999999998	23.962500000000002
70-71	23.0	27.187499999999996	27.5125	22.3
72-73	21.6	28.512500000000003	26.887499999999996	23.0
74-75	21.675	28.425	27.5125	22.3875
76-77	24.4	26.787499999999998	26.8	22.0125
78-79	23.5	25.887500000000003	27.900000000000002	22.7125
80-81	24.6125	24.975	28.537499999999998	21.875
82-83	24.275	26.375	27.9125	21.4375
84-85	21.837500000000002	28.000000000000004	25.3	24.8625
86-87	22.5625	27.950000000000003	27.05	22.4375
88-89	25.324999999999996	25.5	27.8375	21.337500000000002
90-91	24.0125	26.0125	27.3	22.675
92-93	22.125	27.037499999999998	27.975	22.8625
94-95	22.625	27.875	26.724999999999998	22.775000000000002
96-97	22.0	27.775	24.575	25.650000000000002
98-99	22.287499999999998	27.500000000000004	28.3875	21.825
100-101	22.5625	27.0125	28.449999999999996	21.975
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	1.5
21	2.5
22	5.0
23	5.0
24	1.5
25	7.0
26	13.0
27	9.5
28	7.5
29	12.5
30	28.5
31	53.5
32	96.0
33	112.0
34	107.0
35	126.5
36	125.0
37	114.0
38	85.0
39	62.0
40	83.0
41	143.5
42	196.5
43	178.0
44	136.0
45	145.0
46	226.5
47	265.5
48	201.5
49	156.0
50	167.5
51	156.0
52	121.0
53	91.0
54	83.5
55	96.5
56	91.0
57	68.5
58	72.0
59	98.5
60	84.5
61	41.5
62	28.5
63	22.5
64	8.5
65	3.5
66	3.0
67	8.0
68	7.5
69	4.5
70	3.5
71	3.0
72	3.0
73	4.0
74	4.0
75	2.0
76	3.0
77	4.5
78	4.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.075
3	0.075
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.849999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.12921890067503	35.325
2	15.863066538090646	16.45
3	6.171648987463838	9.6
4	2.941176470588235	6.1
5	1.5429122468659595	4.0
6	1.253616200578592	3.9
7	1.0607521697203472	3.85
8	0.5785920925747349	2.4
9	0.43394406943105107	2.025
>10	2.0250723240115716	16.35
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	46	1.15	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	37	0.9249999999999999	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	33	0.8250000000000001	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	25	0.625	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	23	0.575	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	20	0.5	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	19	0.475	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	19	0.475	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	18	0.44999999999999996	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	18	0.44999999999999996	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	18	0.44999999999999996	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	16	0.4	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	16	0.4	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	16	0.4	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	15	0.375	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	15	0.375	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	15	0.375	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	14	0.35000000000000003	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	14	0.35000000000000003	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	14	0.35000000000000003	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	14	0.35000000000000003	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	13	0.325	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	13	0.325	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	12	0.3	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	12	0.3	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	12	0.3	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	12	0.3	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	12	0.3	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	11	0.27499999999999997	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	11	0.27499999999999997	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	11	0.27499999999999997	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	10	0.25	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	10	0.25	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	10	0.25	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	10	0.25	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	10	0.25	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	10	0.25	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	10	0.25	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	10	0.25	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	10	0.25	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	10	0.25	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	10	0.25	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	9	0.22499999999999998	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	9	0.22499999999999998	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	9	0.22499999999999998	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	9	0.22499999999999998	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	9	0.22499999999999998	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	9	0.22499999999999998	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	9	0.22499999999999998	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	9	0.22499999999999998	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	9	0.22499999999999998	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	8	0.2	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	8	0.2	No Hit
CAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATG	8	0.2	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	8	0.2	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	8	0.2	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	8	0.2	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	8	0.2	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	8	0.2	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	8	0.2	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	8	0.2	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	8	0.2	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	8	0.2	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	7	0.17500000000000002	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	7	0.17500000000000002	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	7	0.17500000000000002	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	7	0.17500000000000002	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	7	0.17500000000000002	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	7	0.17500000000000002	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	7	0.17500000000000002	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	7	0.17500000000000002	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	7	0.17500000000000002	No Hit
CTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCTCACAT	7	0.17500000000000002	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	7	0.17500000000000002	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	7	0.17500000000000002	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	7	0.17500000000000002	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	7	0.17500000000000002	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	7	0.17500000000000002	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	7	0.17500000000000002	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	7	0.17500000000000002	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	7	0.17500000000000002	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	7	0.17500000000000002	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	7	0.17500000000000002	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	7	0.17500000000000002	No Hit
CCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGGTGATCAAGAAATCTGAG	7	0.17500000000000002	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	6	0.15	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	6	0.15	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	6	0.15	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	6	0.15	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	6	0.15	No Hit
ACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAA	6	0.15	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	6	0.15	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	6	0.15	No Hit
TTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAA	6	0.15	No Hit
GTGCGTTTCCCTGTGTAGCCAGGGCTGCTCTGGACATCAGGATCTGCCTG	6	0.15	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	6	0.15	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	6	0.15	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	6	0.15	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	6	0.15	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	6	0.15	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	6	0.15	No Hit
GCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTCCTCCTCGCTGT	6	0.15	No Hit
GCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTT	6	0.15	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	6	0.15	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	6	0.15	No Hit
TCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGC	6	0.15	No Hit
CTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGG	6	0.15	No Hit
AAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTCACG	6	0.15	No Hit
CCACAATGAGGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGA	6	0.15	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	6	0.15	No Hit
CCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTA	5	0.125	No Hit
CTTCCCAGCTTTTAATGCCTCTGGAATGGACTGAATAGACTCAATAAATT	5	0.125	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	5	0.125	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	5	0.125	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	5	0.125	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	5	0.125	No Hit
GTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGC	5	0.125	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	5	0.125	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	5	0.125	No Hit
CCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTA	5	0.125	No Hit
GTTCTTTTAAAAAATAAAATATGTTCAAAGCACCCTTGATATAAATTGCC	5	0.125	No Hit
CTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTT	5	0.125	No Hit
CTTGTCATAAAGCTCTTCCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGG	5	0.125	No Hit
GTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTC	5	0.125	No Hit
AATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTT	5	0.125	No Hit
CTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGT	5	0.125	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	5	0.125	No Hit
TGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTAC	5	0.125	No Hit
CCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATC	5	0.125	No Hit
CTGGAATGGACTGAATAGACTCAATAAATTTGTCCAGGGTTGCTTCCCAA	5	0.125	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	5	0.125	No Hit
TCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAA	5	0.125	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	5	0.125	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	5	0.125	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	5	0.125	No Hit
ATAAATCATTGGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGC	5	0.125	No Hit
CCTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATA	5	0.125	No Hit
CTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAGAGATGAGTGCGT	5	0.125	No Hit
GACTTTCTTCCCAGCTTTTAATGCCTCTGGAATGGACTGAATAGACTCAA	5	0.125	No Hit
CTCCCTTTGGCTCCTGTAGGTCGTCCTCTTCTGTCTGGGCCTTGTTCATT	5	0.125	No Hit
TCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTA	5	0.125	No Hit
TTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.0625	0.0	0.0	0.0	0.0
76-77	0.1375	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.275	0.0	0.0	0.0	0.0
82-83	0.3	0.0	0.0	0.0	0.0
84-85	0.325	0.0	0.0	0.0	0.0
86-87	0.375	0.0	0.0	0.0	0.0
88-89	0.5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882428 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882428_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.48725	37.0	37.0	37.0	37.0	37.0
2	36.399	37.0	37.0	37.0	37.0	37.0
3	36.4005	37.0	37.0	37.0	37.0	37.0
4	36.414	37.0	37.0	37.0	37.0	37.0
5	36.418	37.0	37.0	37.0	37.0	37.0
6	36.3265	37.0	37.0	37.0	37.0	37.0
7	36.5685	37.0	37.0	37.0	37.0	37.0
8	36.4915	37.0	37.0	37.0	37.0	37.0
9	36.4865	37.0	37.0	37.0	37.0	37.0
10-11	36.448499999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.460499999999996	37.0	37.0	37.0	37.0	37.0
14-15	36.442	37.0	37.0	37.0	37.0	37.0
16-17	36.48025	37.0	37.0	37.0	37.0	37.0
18-19	36.415499999999994	37.0	37.0	37.0	37.0	37.0
20-21	36.41375	37.0	37.0	37.0	37.0	37.0
22-23	36.504999999999995	37.0	37.0	37.0	37.0	37.0
24-25	36.349999999999994	37.0	37.0	37.0	37.0	37.0
26-27	36.34075	37.0	37.0	37.0	37.0	37.0
28-29	36.369	37.0	37.0	37.0	37.0	37.0
30-31	36.3525	37.0	37.0	37.0	37.0	37.0
32-33	36.337	37.0	37.0	37.0	37.0	37.0
34-35	36.324	37.0	37.0	37.0	37.0	37.0
36-37	36.382999999999996	37.0	37.0	37.0	37.0	37.0
38-39	36.2345	37.0	37.0	37.0	37.0	37.0
40-41	36.23925	37.0	37.0	37.0	37.0	37.0
42-43	36.2365	37.0	37.0	37.0	37.0	37.0
44-45	36.28675	37.0	37.0	37.0	37.0	37.0
46-47	36.2565	37.0	37.0	37.0	37.0	37.0
48-49	36.13475	37.0	37.0	37.0	37.0	37.0
50-51	36.24275	37.0	37.0	37.0	37.0	37.0
52-53	36.218	37.0	37.0	37.0	37.0	37.0
54-55	36.164249999999996	37.0	37.0	37.0	37.0	37.0
56-57	36.17675	37.0	37.0	37.0	37.0	37.0
58-59	36.1455	37.0	37.0	37.0	37.0	37.0
60-61	36.044	37.0	37.0	37.0	37.0	37.0
62-63	36.14275	37.0	37.0	37.0	37.0	37.0
64-65	36.12425	37.0	37.0	37.0	37.0	37.0
66-67	36.078	37.0	37.0	37.0	37.0	37.0
68-69	36.06425	37.0	37.0	37.0	37.0	37.0
70-71	35.993750000000006	37.0	37.0	37.0	37.0	37.0
72-73	36.0745	37.0	37.0	37.0	37.0	37.0
74-75	36.02425	37.0	37.0	37.0	37.0	37.0
76-77	36.099000000000004	37.0	37.0	37.0	37.0	37.0
78-79	36.07225	37.0	37.0	37.0	37.0	37.0
80-81	35.96625	37.0	37.0	37.0	37.0	37.0
82-83	35.9835	37.0	37.0	37.0	37.0	37.0
84-85	35.8745	37.0	37.0	37.0	37.0	37.0
86-87	35.91	37.0	37.0	37.0	37.0	37.0
88-89	35.93175	37.0	37.0	37.0	37.0	37.0
90-91	35.828500000000005	37.0	37.0	37.0	37.0	37.0
92-93	35.850750000000005	37.0	37.0	37.0	37.0	37.0
94-95	35.91225	37.0	37.0	37.0	37.0	37.0
96-97	35.792	37.0	37.0	37.0	37.0	37.0
98-99	35.85825	37.0	37.0	37.0	37.0	37.0
100-101	35.51225	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	4.0
26	1.0
27	8.0
28	13.0
29	15.0
30	15.0
31	31.0
32	36.0
33	77.0
34	139.0
35	495.0
36	2764.0
37	400.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.070122714750816	19.684447783621337	13.749060856498874	26.496368645128975
2	20.825	27.0	34.300000000000004	17.875
3	20.5	27.6	31.324999999999996	20.575
4	25.224999999999998	34.125	20.849999999999998	19.8
5	23.599999999999998	34.475	23.525	18.4
6	22.225	36.375	23.0	18.4
7	22.6	21.45	35.55	20.4
8	22.1	21.5	30.25	26.150000000000002
9	23.325000000000003	25.025	27.500000000000004	24.15
10-11	24.925	32.2625	22.05	20.7625
12-13	26.125	24.7875	26.7125	22.375
14-15	23.5875	27.1125	28.1	21.2
16-17	24.2	27.962500000000002	27.950000000000003	19.8875
18-19	22.55	28.1375	27.5625	21.75
20-21	22.8625	30.062499999999996	26.8	20.275000000000002
22-23	23.6875	29.799999999999997	25.2	21.3125
24-25	24.5375	28.975	25.75	20.7375
26-27	22.7625	28.962500000000002	26.387500000000003	21.8875
28-29	24.3625	27.05	26.9125	21.675
30-31	24.3	26.924999999999997	26.887499999999996	21.8875
32-33	24.725	26.887499999999996	28.249999999999996	20.1375
34-35	23.549999999999997	27.950000000000003	25.9625	22.537499999999998
36-37	22.625	28.175	28.349999999999998	20.849999999999998
38-39	22.787499999999998	28.0875	26.200000000000003	22.925
40-41	22.900000000000002	27.425	27.325	22.35
42-43	22.400000000000002	28.9	27.05	21.65
44-45	23.1875	27.750000000000004	29.425	19.6375
46-47	23.1875	29.025000000000002	24.825	22.9625
48-49	23.200000000000003	28.499999999999996	27.6375	20.6625
50-51	22.112499999999997	28.000000000000004	26.275	23.6125
52-53	23.0375	27.962500000000002	27.775	21.224999999999998
54-55	25.1	27.187499999999996	25.6125	22.1
56-57	21.762500000000003	27.500000000000004	27.6125	23.125
58-59	22.325	29.462500000000002	25.924999999999997	22.287499999999998
60-61	21.1625	28.775000000000002	27.200000000000003	22.8625
62-63	23.3	27.525	25.4625	23.7125
64-65	23.1125	27.075	29.312500000000004	20.5
66-67	21.987499999999997	25.874999999999996	28.8875	23.25
68-69	23.25	29.25	25.937500000000004	21.5625
70-71	21.349999999999998	29.1625	28.037499999999998	21.45
72-73	23.7125	28.249999999999996	24.875	23.1625
74-75	24.1875	26.700000000000003	27.250000000000004	21.8625
76-77	23.0	27.8625	25.937500000000004	23.200000000000003
78-79	23.575	27.9125	27.1	21.4125
80-81	22.8	27.474999999999998	27.1125	22.6125
82-83	21.925	26.5625	27.6625	23.849999999999998
84-85	21.3875	28.199999999999996	27.6	22.8125
86-87	21.925	29.037499999999998	26.2625	22.775000000000002
88-89	23.5125	28.0625	26.700000000000003	21.725
90-91	23.2125	27.5625	26.224999999999998	23.0
92-93	24.65	28.975	26.4125	19.9625
94-95	22.6125	30.162499999999998	26.5125	20.7125
96-97	23.575	29.062500000000004	25.2375	22.125
98-99	22.8625	28.287499999999998	26.150000000000002	22.7
100-101	24.2625	27.325	26.337500000000002	22.075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.5
25	1.0
26	0.5
27	2.0
28	4.5
29	11.0
30	26.5
31	85.5
32	140.5
33	124.5
34	102.5
35	81.5
36	83.5
37	111.0
38	98.0
39	88.0
40	114.0
41	144.0
42	194.5
43	208.5
44	196.0
45	202.0
46	206.0
47	227.0
48	204.0
49	169.5
50	144.5
51	112.5
52	96.5
53	102.0
54	97.5
55	114.0
56	98.5
57	48.0
58	67.5
59	74.5
60	48.5
61	30.5
62	24.5
63	14.0
64	4.0
65	2.0
66	1.5
67	2.5
68	6.0
69	7.0
70	4.5
71	9.0
72	9.0
73	6.5
74	10.0
75	6.5
76	6.0
77	11.0
78	8.0
79	2.5
80	1.5
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.79789666209419	35.975
2	17.695473251028808	19.35
3	6.355738454503887	10.424999999999999
4	4.206675811614083	9.2
5	1.7375400091449476	4.75
6	1.2802926383173296	4.2
7	1.0516689529035208	4.025
8	0.36579789666209417	1.6
9	0.3200731595793324	1.575
>10	1.188843164151806	8.9
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	33	0.8250000000000001	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	26	0.65	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	17	0.42500000000000004	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	17	0.42500000000000004	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	16	0.4	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	16	0.4	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	14	0.35000000000000003	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	14	0.35000000000000003	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	14	0.35000000000000003	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	14	0.35000000000000003	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	13	0.325	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	13	0.325	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	12	0.3	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	11	0.27499999999999997	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	11	0.27499999999999997	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	11	0.27499999999999997	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	11	0.27499999999999997	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	11	0.27499999999999997	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	11	0.27499999999999997	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	11	0.27499999999999997	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	10	0.25	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	10	0.25	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	10	0.25	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	10	0.25	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	10	0.25	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	10	0.25	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	9	0.22499999999999998	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	9	0.22499999999999998	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	9	0.22499999999999998	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	9	0.22499999999999998	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	9	0.22499999999999998	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	9	0.22499999999999998	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	9	0.22499999999999998	No Hit
GGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGA	8	0.2	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	8	0.2	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	8	0.2	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	8	0.2	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	8	0.2	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	8	0.2	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	8	0.2	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	8	0.2	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	7	0.17500000000000002	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	7	0.17500000000000002	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	7	0.17500000000000002	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	7	0.17500000000000002	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	7	0.17500000000000002	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	7	0.17500000000000002	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	7	0.17500000000000002	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	7	0.17500000000000002	No Hit
CAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAA	7	0.17500000000000002	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	7	0.17500000000000002	No Hit
ATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGT	7	0.17500000000000002	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	7	0.17500000000000002	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	7	0.17500000000000002	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	7	0.17500000000000002	No Hit
AATTTATTGAGTCTATTCAGTCCATTCCAGAGGCATTAAAAGCTGGGAAG	7	0.17500000000000002	No Hit
GTCAAACTGTCTCATAAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGC	7	0.17500000000000002	No Hit
TTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACAC	7	0.17500000000000002	No Hit
GGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTG	7	0.17500000000000002	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	7	0.17500000000000002	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	7	0.17500000000000002	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	7	0.17500000000000002	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	7	0.17500000000000002	No Hit
CCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAATG	7	0.17500000000000002	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	6	0.15	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	6	0.15	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	6	0.15	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	6	0.15	No Hit
CGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGT	6	0.15	No Hit
AATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGC	6	0.15	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	6	0.15	No Hit
TGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATG	6	0.15	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	6	0.15	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	6	0.15	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	6	0.15	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	6	0.15	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	6	0.15	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	6	0.15	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	6	0.15	No Hit
TACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTT	6	0.15	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	6	0.15	No Hit
ATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTG	6	0.15	No Hit
AATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAAT	6	0.15	No Hit
CACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAA	6	0.15	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	6	0.15	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	6	0.15	No Hit
TTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTTTGA	6	0.15	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	6	0.15	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	6	0.15	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	6	0.15	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	6	0.15	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	6	0.15	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	5	0.125	No Hit
GAAGAACTCAACTACATAAAGACAGAGGGACAATCAAAACTGCACAGAGG	5	0.125	No Hit
TGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTG	5	0.125	No Hit
CCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTT	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	5	0.125	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	5	0.125	No Hit
GTTGGACAGAGAGAGTCAGAAGATGTGAAAGAAAAAGACCGAGCTAAAGA	5	0.125	No Hit
CTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGC	5	0.125	No Hit
GTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAG	5	0.125	No Hit
TCCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGAC	5	0.125	No Hit
CCACATGCCAGGTTATCAAGCACCAGCAGCTGATGGAACAGGCTGTGGCC	5	0.125	No Hit
GCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAA	5	0.125	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	5	0.125	No Hit
ATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTCATGTACTTATTG	5	0.125	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	5	0.125	No Hit
CTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTG	5	0.125	No Hit
AGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCC	5	0.125	No Hit
GTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGA	5	0.125	No Hit
CTTGGATGACGCCATTCTAGAGAAATTTGCGTTCTCCAACGCTCTCTGCC	5	0.125	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	5	0.125	No Hit
GACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGA	5	0.125	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	5	0.125	No Hit
CATCCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCT	5	0.125	No Hit
GCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGC	5	0.125	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	5	0.125	No Hit
AAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGCCCTCAGGCACCGAAT	5	0.125	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	5	0.125	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	5	0.125	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	5	0.125	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	5	0.125	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	5	0.125	No Hit
ATTCAGTCCATTCCAGAGGCATTAAAAGCTGGGAAGAAAGTCAAACTGTC	5	0.125	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	5	0.125	No Hit
GCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTGAAATACG	5	0.125	No Hit
GAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAA	5	0.125	No Hit
CGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTT	5	0.125	No Hit
GCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.0625	0.0	0.0	0.0	0.0
76-77	0.125	0.0	0.0	0.0	0.0
78-79	0.1875	0.0	0.0	0.0	0.0
80-81	0.225	0.0	0.0	0.0	0.0
82-83	0.25	0.0	0.0	0.0	0.0
84-85	0.275	0.0	0.0	0.0	0.0
86-87	0.325	0.0	0.0	0.0	0.0
88-89	0.45	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
Read 3928541 spots for SRR24882428.sra
Written 3928541 spots for SRR24882428.sra
Read 3928538 spots for SRR24882428.sra
Written 3928538 spots for SRR24882428.sra
SRR ids: ['SRR24882428.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_t175iiu_
SRR24882428.sra spots: 31428307
blocks: [[1, 3928538], [3928539, 7857076], [7857077, 11785614], [11785615, 15714152], [15714153, 19642690], [19642691, 23571228], [23571229, 27499766], [27499767, 31428307]]
SRR24882428 file size 7589848
SRR24882428 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882428 SRR24882428_1.fastq SRR24882428_2.fastq
Input file:	SRR24882428_1.fastq
Paired file:	SRR24882428_2.fastq
trimmed:	SRR24882428-trimmed-pair1.fastq, SRR24882428-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 01:55:15 2026 >> started

Fri Jul  3 01:55:50 2026 >> done (35.908s)
31428307 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
31428307 (100.00%) read pairs available; of these:
  743209 ( 2.36%) trimmed read pairs available after processing
30685098 (97.64%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 63	       2	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	      25	  0.00%
 67	    1040	  0.00%
 68	    5190	  0.02%
 69	    6470	  0.02%
 70	    7104	  0.02%
 71	    8071	  0.03%
 72	    9180	  0.03%
 73	    9434	  0.03%
 74	   10233	  0.03%
 75	   11377	  0.04%
 76	   12151	  0.04%
 77	   13151	  0.04%
 78	   13726	  0.04%
 79	   14569	  0.05%
 80	   15358	  0.05%
 81	   16544	  0.05%
 82	   17426	  0.06%
 83	   18571	  0.06%
 84	   19818	  0.06%
 85	   21099	  0.07%
 86	   21348	  0.07%
 87	   22973	  0.07%
 88	   24624	  0.08%
 89	   25156	  0.08%
 90	   27383	  0.09%
 91	   29760	  0.09%
 92	   30223	  0.10%
 93	   32240	  0.10%
 94	   33779	  0.11%
 95	   35491	  0.11%
 96	   37251	  0.12%
 97	   40178	  0.13%
 98	   41361	  0.13%
 99	   45314	  0.14%
100	   65588	  0.21%
101	30685098	 97.64%
31428307 reads passed initial QC


criterion=sequence-density
sequence-density=1.17
sequence-density-rank=1
fanout-score=2.77
fanout-score-rank=30
prefix-density=1.32
prefix-fanout=2.5
sequence=GATGGAGAAGATG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=35
fanout-score=52.14
fanout-score-rank=1
prefix-density=0.52
prefix-fanout=2.1
sequence=CAAAGATCTTCACATTTTGCTCATCTCTTTGGTCACTTTCCTGCACTTGGATCAATCACTCTTCGCTTATGATTTTGGTGTGAACCTCCCGGCTCTTCACCCGCAGCTTGTTGACCTGGGACTCCGCGATGTCAGCCCGCTCCTCGGCTTCCTCCAGCTCGTGCTGGATCTTGCGGAA


criterion=sequence-density
sequence-density=1.24
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=33
prefix-density=1.22
prefix-fanout=2.0
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.25
sequence-density-rank=13
fanout-score=26.02
fanout-score-rank=1
prefix-density=1.08
prefix-fanout=6.1
sequence=GAGGAGCTGGAGGAGG
SRR24882428 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 01:56:12
                             Started mapping on |	Jul 03 01:56:12
                                    Finished on |	Jul 03 02:18:15
       Mapping speed, Million of reads per hour |	85.52

                          Number of input reads |	31428307
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27921755
                        Uniquely mapped reads % |	88.84%
                          Average mapped length |	200.31
                       Number of splices: Total |	18795125
            Number of splices: Annotated (sjdb) |	18645701
                       Number of splices: GT/AG |	18653479
                       Number of splices: GC/AG |	110807
                       Number of splices: AT/AC |	11228
               Number of splices: Non-canonical |	19611
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.03%
                        Deletion average length |	4.18
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.73
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3499899
             % of reads mapped to multiple loci |	11.14%
        Number of reads mapped to too many loci |	2648
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6653	6653	6653
N_multimapping	3499899	3499899	3499899
N_noFeature	2353977	24128375	3425209
N_ambiguous	2975509	1415930	247889
UnstrandedReadsAssigned:22592269 PositiveStrandReadsAssigned:2377450 NegativeStrandReadsAssigned:24248657
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882428 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882428-trimmed-pair1.fastq
                             SRR24882428-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,428,307 reads, 25,166,685 reads pseudoaligned
[quant] estimated average fragment length: 195.947
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,162 rounds

 109283 SRR24882428.ke.tsv
  52636 SRR24882428.se.tsv
 161919 total
==> SRR24882428.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9.4	0	0
ENSMUST00000196221.1	9	4.66667	0	0
ENSMUST00000179664.1	11	4.4	0	0
ENSMUST00000178537.1	12	5.4	0	0
ENSMUST00000178862.1	14	7.4	0	0
ENSMUST00000179520.1	11	4.4	0	0
ENSMUST00000179883.1	16	9.4	0	0
ENSMUST00000195858.1	10	5.66667	0	0
ENSMUST00000179932.1	12	5.4	0	0

==> SRR24882428.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	4
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	1
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882428 completed mapping pipeline successfully
