Starting /dee2/code/volunteer_pipeline.sh SRR24882430
    current disk space = 1364211448
    free memory = 195989404 
SRR24882430 SRAfilesize
6807e6195c9f0fe6fc53f8523022f254  SRR24882430.sra
SRR24882430.sra file validated
SRR24882430 is paired end
SRR24882430 is conventional basespace
SRR24882430 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.62525	37.0	37.0	37.0	37.0	37.0
2	36.51225	37.0	37.0	37.0	37.0	37.0
3	36.5815	37.0	37.0	37.0	37.0	37.0
4	36.67	37.0	37.0	37.0	37.0	37.0
5	36.6675	37.0	37.0	37.0	37.0	37.0
6	36.634	37.0	37.0	37.0	37.0	37.0
7	36.656	37.0	37.0	37.0	37.0	37.0
8	36.624	37.0	37.0	37.0	37.0	37.0
9	36.674	37.0	37.0	37.0	37.0	37.0
10-11	36.5925	37.0	37.0	37.0	37.0	37.0
12-13	36.6365	37.0	37.0	37.0	37.0	37.0
14-15	36.5805	37.0	37.0	37.0	37.0	37.0
16-17	36.6425	37.0	37.0	37.0	37.0	37.0
18-19	36.66475	37.0	37.0	37.0	37.0	37.0
20-21	36.68075	37.0	37.0	37.0	37.0	37.0
22-23	36.6285	37.0	37.0	37.0	37.0	37.0
24-25	36.6125	37.0	37.0	37.0	37.0	37.0
26-27	36.61	37.0	37.0	37.0	37.0	37.0
28-29	36.57725	37.0	37.0	37.0	37.0	37.0
30-31	36.5785	37.0	37.0	37.0	37.0	37.0
32-33	36.544875000000005	37.0	37.0	37.0	37.0	37.0
34-35	36.549	37.0	37.0	37.0	37.0	37.0
36-37	36.491749999999996	37.0	37.0	37.0	37.0	37.0
38-39	36.4755	37.0	37.0	37.0	37.0	37.0
40-41	36.54675	37.0	37.0	37.0	37.0	37.0
42-43	36.46925	37.0	37.0	37.0	37.0	37.0
44-45	36.52825	37.0	37.0	37.0	37.0	37.0
46-47	36.542500000000004	37.0	37.0	37.0	37.0	37.0
48-49	36.42675	37.0	37.0	37.0	37.0	37.0
50-51	36.44725	37.0	37.0	37.0	37.0	37.0
52-53	36.46575	37.0	37.0	37.0	37.0	37.0
54-55	36.50425	37.0	37.0	37.0	37.0	37.0
56-57	36.41225	37.0	37.0	37.0	37.0	37.0
58-59	36.4885	37.0	37.0	37.0	37.0	37.0
60-61	36.389	37.0	37.0	37.0	37.0	37.0
62-63	36.477	37.0	37.0	37.0	37.0	37.0
64-65	36.3875	37.0	37.0	37.0	37.0	37.0
66-67	36.403000000000006	37.0	37.0	37.0	37.0	37.0
68-69	36.431	37.0	37.0	37.0	37.0	37.0
70-71	36.39575000000001	37.0	37.0	37.0	37.0	37.0
72-73	36.384	37.0	37.0	37.0	37.0	37.0
74-75	36.3705	37.0	37.0	37.0	37.0	37.0
76-77	36.31975	37.0	37.0	37.0	37.0	37.0
78-79	36.32	37.0	37.0	37.0	37.0	37.0
80-81	36.367000000000004	37.0	37.0	37.0	37.0	37.0
82-83	36.238	37.0	37.0	37.0	37.0	37.0
84-85	36.328	37.0	37.0	37.0	37.0	37.0
86-87	36.2915	37.0	37.0	37.0	37.0	37.0
88-89	36.30625	37.0	37.0	37.0	37.0	37.0
90-91	36.211	37.0	37.0	37.0	37.0	37.0
92-93	36.30825	37.0	37.0	37.0	37.0	37.0
94-95	36.24325	37.0	37.0	37.0	37.0	37.0
96-97	36.179249999999996	37.0	37.0	37.0	37.0	37.0
98-99	36.188	37.0	37.0	37.0	37.0	37.0
100-101	36.03175	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	0.0
26	2.0
27	3.0
28	6.0
29	12.0
30	18.0
31	20.0
32	27.0
33	39.0
34	80.0
35	263.0
36	2689.0
37	840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.60915228807202	11.352838209552388	11.0527631907977	40.985246311577896
2	18.904726181545385	14.553638409602401	37.28432108027007	29.257314328582147
3	18.12719078617927	18.052078117175764	31.72258387581372	32.098147220831244
4	24.65	27.05	23.7	24.6
5	25.4	28.775000000000002	24.65	21.175
6	20.674999999999997	32.475	28.15	18.7
7	16.55	21.825	45.050000000000004	16.575
8	20.375	22.400000000000002	33.625	23.599999999999998
9	21.224999999999998	23.35	33.15	22.275
10-11	21.637500000000003	31.2625	25.4375	21.6625
12-13	22.775000000000002	24.75	29.212500000000002	23.2625
14-15	19.6125	27.8125	30.8	21.775
16-17	20.4125	26.950000000000003	28.212500000000002	24.425
18-19	22.05	28.712500000000002	26.4125	22.825
20-21	22.162499999999998	26.737499999999997	27.950000000000003	23.150000000000002
22-23	21.9625	26.55	27.85	23.6375
24-25	22.6375	27.1375	27.5625	22.662499999999998
26-27	22.625	27.237499999999997	28.787499999999998	21.349999999999998
28-29	21.587500000000002	27.0	28.9125	22.5
30-31	22.162499999999998	26.224999999999998	29.025000000000002	22.5875
32-33	21.352669083635455	25.053131641455185	30.466308288536066	23.1278909863733
34-35	19.8875	27.05	28.487499999999997	24.575
36-37	22.7	27.3375	29.762499999999996	20.200000000000003
38-39	21.475	27.55	30.175	20.8
40-41	21.3125	28.4	29.5	20.7875
42-43	21.75	29.312500000000004	28.199999999999996	20.7375
44-45	19.8875	28.6625	28.325	23.125
46-47	22.925	27.8125	25.687500000000004	23.575
48-49	20.3	30.049999999999997	27.85	21.8
50-51	21.912499999999998	25.2375	29.262500000000003	23.5875
52-53	21.275	26.4625	28.749999999999996	23.5125
54-55	20.6375	26.775	28.262500000000003	24.325
56-57	19.575	27.675	29.312500000000004	23.4375
58-59	21.8	25.75	28.549999999999997	23.9
60-61	20.200000000000003	28.475	27.775	23.549999999999997
62-63	22.8	27.4125	27.0	22.787499999999998
64-65	22.7375	25.724999999999998	27.8875	23.65
66-67	23.150000000000002	25.624999999999996	29.037499999999998	22.1875
68-69	21.9625	26.125	27.700000000000003	24.212500000000002
70-71	23.5875	27.025	26.950000000000003	22.4375
72-73	20.8125	29.175	27.6125	22.400000000000002
74-75	22.237499999999997	27.250000000000004	28.1125	22.400000000000002
76-77	24.212500000000002	27.075	26.5375	22.175
78-79	23.5625	26.474999999999998	28.075	21.8875
80-81	24.6625	26.5625	27.250000000000004	21.525
82-83	24.2875	27.075	28.075	20.5625
84-85	22.275	28.499999999999996	25.337500000000002	23.8875
86-87	21.7375	28.3125	27.487499999999997	22.4625
88-89	24.525	27.025	27.3625	21.087500000000002
90-91	22.525000000000002	26.825	28.4375	22.2125
92-93	22.162499999999998	26.400000000000002	28.025	23.4125
94-95	22.875	27.325	27.5125	22.287499999999998
96-97	20.775	28.012500000000003	26.625	24.587500000000002
98-99	21.762500000000003	26.5125	29.425	22.3
100-101	21.5	27.775	27.6875	23.0375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.0
20	1.0
21	2.0
22	1.5
23	2.5
24	2.5
25	11.5
26	13.5
27	5.0
28	7.5
29	13.0
30	28.5
31	54.0
32	94.5
33	120.5
34	119.0
35	131.0
36	118.5
37	99.0
38	90.0
39	67.5
40	76.5
41	136.0
42	215.0
43	204.0
44	147.0
45	156.5
46	225.5
47	264.0
48	206.0
49	160.5
50	174.0
51	164.0
52	130.0
53	112.5
54	98.0
55	78.5
56	63.5
57	47.5
58	56.5
59	86.0
60	70.0
61	33.0
62	27.0
63	23.0
64	10.0
65	4.5
66	2.0
67	5.5
68	7.5
69	5.0
70	3.5
71	2.5
72	1.0
73	1.5
74	4.0
75	3.0
76	1.0
77	3.0
78	3.5
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.025
3	0.15
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0125
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.13681241184767	35.699999999999996
2	17.395392571697226	18.5
3	5.6417489421720735	9.0
4	2.8678890456041373	6.1
5	2.0216267042783262	5.375
6	1.457451810061119	4.65
7	0.7052186177715092	2.625
8	0.611189468735308	2.6
9	0.37611659614480486	1.7999999999999998
>10	1.7865538316878231	13.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	41	1.0250000000000001	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	27	0.675	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	23	0.575	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	21	0.525	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	21	0.525	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	21	0.525	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	19	0.475	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	17	0.42500000000000004	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	16	0.4	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	16	0.4	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	15	0.375	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	15	0.375	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	14	0.35000000000000003	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	14	0.35000000000000003	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	14	0.35000000000000003	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	13	0.325	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	13	0.325	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	13	0.325	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	12	0.3	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	12	0.3	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	12	0.3	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	12	0.3	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	12	0.3	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	11	0.27499999999999997	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	11	0.27499999999999997	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	11	0.27499999999999997	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	10	0.25	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	10	0.25	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	10	0.25	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	10	0.25	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	10	0.25	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	10	0.25	No Hit
CGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAAC	10	0.25	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	10	0.25	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	10	0.25	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	10	0.25	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	10	0.25	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	10	0.25	No Hit
GGCTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTA	9	0.22499999999999998	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	9	0.22499999999999998	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	9	0.22499999999999998	No Hit
GTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCGGAAGGTCTTGTTTGGG	9	0.22499999999999998	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	9	0.22499999999999998	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	9	0.22499999999999998	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	9	0.22499999999999998	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	9	0.22499999999999998	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	8	0.2	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	8	0.2	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	8	0.2	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	8	0.2	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	8	0.2	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	8	0.2	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	8	0.2	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	8	0.2	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	8	0.2	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	8	0.2	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	8	0.2	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	8	0.2	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	8	0.2	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	7	0.17500000000000002	No Hit
GCCAGTGTGGTGCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAG	7	0.17500000000000002	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	7	0.17500000000000002	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	7	0.17500000000000002	No Hit
GCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGG	7	0.17500000000000002	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	7	0.17500000000000002	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	7	0.17500000000000002	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	7	0.17500000000000002	No Hit
GAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATT	7	0.17500000000000002	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	7	0.17500000000000002	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	7	0.17500000000000002	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	7	0.17500000000000002	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	7	0.17500000000000002	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	7	0.17500000000000002	No Hit
AGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTT	7	0.17500000000000002	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	6	0.15	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	6	0.15	No Hit
CTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCT	6	0.15	No Hit
AAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAA	6	0.15	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	6	0.15	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	6	0.15	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	6	0.15	No Hit
GCTTGATTTCCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAG	6	0.15	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	6	0.15	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	6	0.15	No Hit
AGGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAAT	6	0.15	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	6	0.15	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	6	0.15	No Hit
GCCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	6	0.15	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	6	0.15	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	6	0.15	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	6	0.15	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	6	0.15	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	6	0.15	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	6	0.15	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	6	0.15	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	6	0.15	No Hit
CTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTA	6	0.15	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	6	0.15	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	6	0.15	No Hit
CCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGT	6	0.15	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	6	0.15	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	6	0.15	No Hit
CATGTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAA	6	0.15	No Hit
GTGGGTCATGCTCCTGTGAGTCCCAAAGGGGTGCATGCTTGGCCAGTGTT	6	0.15	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	6	0.15	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	5	0.125	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	5	0.125	No Hit
CACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTAT	5	0.125	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	5	0.125	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	5	0.125	No Hit
CTTTACTGCTTTTATAAACCACTTAGAACACAAGAGATTTGGTTTGTGGG	5	0.125	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	5	0.125	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	5	0.125	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	5	0.125	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	5	0.125	No Hit
CTCACCCATTTTCTGCATAACTTCTTTATGAGACAGTTTGACTTTCTTCC	5	0.125	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	5	0.125	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	5	0.125	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	5	0.125	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	5	0.125	No Hit
GGGTCATGCTCCTGTGAGTCCCAAAGGGGTGCATGCTTGGCCAGTGTTCT	5	0.125	No Hit
GAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATG	5	0.125	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	5	0.125	No Hit
ATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCT	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	5	0.125	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	5	0.125	No Hit
CTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATAT	5	0.125	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	5	0.125	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	5	0.125	No Hit
CTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAG	5	0.125	No Hit
GCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTTT	5	0.125	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	5	0.125	No Hit
AAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTTTTTTTTTAAGAA	5	0.125	No Hit
GAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGG	5	0.125	No Hit
GGTCATGCTCCTGTGAGTCCCAAAGGGGTGCATGCTTGGCCAGTGTTCTT	5	0.125	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	5	0.125	No Hit
GGGGACTGGACATAGGAGTAGGATATATTAGACATATTATATAAATTAGA	5	0.125	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	5	0.125	No Hit
GTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTTGCACTTGCCCGGC	5	0.125	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	5	0.125	No Hit
GCCTGATGTGGTGATCTGTCCATGGGCTTCTAACACCGCCTCCCCCGCGA	5	0.125	No Hit
CTTGATTTCCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAGT	5	0.125	No Hit
CCGCATTAGGTCTGTTAGTTCCATGCAGTGCTGCAGTTTTTCATTCATGA	5	0.125	No Hit
CCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAGTTGAGTTCT	5	0.125	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	5	0.125	No Hit
CAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTCCTCCTCGCTGTT	5	0.125	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	5	0.125	No Hit
CTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAGAGATGAGTGCGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.025	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.1	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.325	0.0	0.0	0.0	0.0
82-83	0.42500000000000004	0.0	0.0	0.0	0.0
84-85	0.7250000000000001	0.0	0.0	0.0	0.0
86-87	1.1125	0.0	0.0	0.0	0.0
88-89	1.3375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGCTG	15	0.009957196	47.5	82-83
>>END_MODULE
SRR24882430 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882430_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.474	37.0	37.0	37.0	37.0	37.0
2	36.359	37.0	37.0	37.0	37.0	37.0
3	36.49	37.0	37.0	37.0	37.0	37.0
4	36.4575	37.0	37.0	37.0	37.0	37.0
5	36.4945	37.0	37.0	37.0	37.0	37.0
6	36.5435	37.0	37.0	37.0	37.0	37.0
7	36.4195	37.0	37.0	37.0	37.0	37.0
8	36.5415	37.0	37.0	37.0	37.0	37.0
9	36.5865	37.0	37.0	37.0	37.0	37.0
10-11	36.478750000000005	37.0	37.0	37.0	37.0	37.0
12-13	36.48375	37.0	37.0	37.0	37.0	37.0
14-15	36.510000000000005	37.0	37.0	37.0	37.0	37.0
16-17	36.47675	37.0	37.0	37.0	37.0	37.0
18-19	36.44725	37.0	37.0	37.0	37.0	37.0
20-21	36.4655	37.0	37.0	37.0	37.0	37.0
22-23	36.40675	37.0	37.0	37.0	37.0	37.0
24-25	36.37475	37.0	37.0	37.0	37.0	37.0
26-27	36.395	37.0	37.0	37.0	37.0	37.0
28-29	36.408	37.0	37.0	37.0	37.0	37.0
30-31	36.39775	37.0	37.0	37.0	37.0	37.0
32-33	36.391999999999996	37.0	37.0	37.0	37.0	37.0
34-35	36.38175	37.0	37.0	37.0	37.0	37.0
36-37	36.41525	37.0	37.0	37.0	37.0	37.0
38-39	36.36575	37.0	37.0	37.0	37.0	37.0
40-41	36.320750000000004	37.0	37.0	37.0	37.0	37.0
42-43	36.36025	37.0	37.0	37.0	37.0	37.0
44-45	36.3275	37.0	37.0	37.0	37.0	37.0
46-47	36.251999999999995	37.0	37.0	37.0	37.0	37.0
48-49	36.18825	37.0	37.0	37.0	37.0	37.0
50-51	36.3075	37.0	37.0	37.0	37.0	37.0
52-53	36.261	37.0	37.0	37.0	37.0	37.0
54-55	36.28575	37.0	37.0	37.0	37.0	37.0
56-57	36.27875	37.0	37.0	37.0	37.0	37.0
58-59	36.22325	37.0	37.0	37.0	37.0	37.0
60-61	36.16	37.0	37.0	37.0	37.0	37.0
62-63	36.22225	37.0	37.0	37.0	37.0	37.0
64-65	36.216750000000005	37.0	37.0	37.0	37.0	37.0
66-67	36.12425	37.0	37.0	37.0	37.0	37.0
68-69	36.183	37.0	37.0	37.0	37.0	37.0
70-71	36.027249999999995	37.0	37.0	37.0	37.0	37.0
72-73	36.036249999999995	37.0	37.0	37.0	37.0	37.0
74-75	36.12	37.0	37.0	37.0	37.0	37.0
76-77	36.057500000000005	37.0	37.0	37.0	37.0	37.0
78-79	36.05375	37.0	37.0	37.0	37.0	37.0
80-81	36.041	37.0	37.0	37.0	37.0	37.0
82-83	35.9965	37.0	37.0	37.0	37.0	37.0
84-85	36.01275	37.0	37.0	37.0	37.0	37.0
86-87	35.9945	37.0	37.0	37.0	37.0	37.0
88-89	35.896	37.0	37.0	37.0	37.0	37.0
90-91	35.96925	37.0	37.0	37.0	37.0	37.0
92-93	35.90075	37.0	37.0	37.0	37.0	37.0
94-95	35.88275	37.0	37.0	37.0	37.0	37.0
96-97	35.8995	37.0	37.0	37.0	37.0	37.0
98-99	35.783249999999995	37.0	37.0	37.0	37.0	37.0
100-101	35.482749999999996	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	1.0
26	4.0
27	8.0
28	13.0
29	17.0
30	16.0
31	25.0
32	29.0
33	62.0
34	125.0
35	459.0
36	2759.0
37	480.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.6265664160401	18.82205513784461	12.631578947368421	27.919799498746865
2	21.775	27.325	33.15	17.75
3	21.175	27.474999999999998	31.15	20.200000000000003
4	23.875	33.900000000000006	21.95	20.275000000000002
5	24.275	33.75	22.55	19.425
6	21.7	35.375	22.875	20.05
7	21.05	21.85	36.125	20.974999999999998
8	21.825	23.075000000000003	29.5	25.6
9	23.825	24.025	28.625	23.525
10-11	24.2375	32.4375	22.6375	20.6875
12-13	25.424999999999997	26.224999999999998	25.8	22.55
14-15	24.1125	26.224999999999998	28.375	21.2875
16-17	24.4875	29.625	26.700000000000003	19.1875
18-19	23.3875	29.262500000000003	26.924999999999997	20.424999999999997
20-21	23.025000000000002	30.175	27.025	19.775000000000002
22-23	23.7	28.549999999999997	26.25	21.5
24-25	24.425	28.1	26.387500000000003	21.087500000000002
26-27	23.65	26.8125	27.125	22.412499999999998
28-29	24.275	27.150000000000002	27.975	20.599999999999998
30-31	23.7	26.6125	27.9375	21.75
32-33	23.425	25.6125	28.875	22.0875
34-35	22.7625	27.325	26.9125	23.0
36-37	22.9375	27.750000000000004	28.075	21.2375
38-39	23.474999999999998	26.1625	28.1125	22.25
40-41	21.7875	28.6625	28.175	21.375
42-43	23.6875	28.3625	26.7625	21.1875
44-45	23.2875	28.262500000000003	28.449999999999996	20.0
46-47	23.8875	27.537499999999998	25.45	23.125
48-49	23.3625	26.775	29.1625	20.7
50-51	22.225	27.037499999999998	26.700000000000003	24.0375
52-53	23.25	27.6875	27.787499999999998	21.275
54-55	25.7375	28.712500000000002	24.4375	21.1125
56-57	21.0	29.1125	27.150000000000002	22.7375
58-59	22.237499999999997	28.075	26.450000000000003	23.2375
60-61	21.0125	28.9	27.212500000000002	22.875
62-63	22.8	28.499999999999996	25.7375	22.9625
64-65	22.900000000000002	28.175	28.1125	20.8125
66-67	21.912499999999998	27.250000000000004	27.0875	23.75
68-69	24.087500000000002	28.7375	25.912499999999998	21.2625
70-71	21.8625	29.7	27.3375	21.099999999999998
72-73	23.525	28.7375	25.937500000000004	21.8
74-75	22.9375	27.675	27.150000000000002	22.237499999999997
76-77	22.875	28.1	26.125	22.900000000000002
78-79	23.3625	28.1	27.425	21.1125
80-81	22.412499999999998	28.4	27.8375	21.349999999999998
82-83	22.1875	28.287499999999998	28.425	21.099999999999998
84-85	21.224999999999998	27.962500000000002	27.2625	23.549999999999997
86-87	22.5	29.025000000000002	26.025	22.45
88-89	22.95	27.900000000000002	27.224999999999998	21.925
90-91	24.1125	27.425	26.3125	22.15
92-93	23.25	30.2125	25.937500000000004	20.599999999999998
94-95	23.0375	29.925	25.525	21.512500000000003
96-97	24.7	27.450000000000003	25.074999999999996	22.775000000000002
98-99	22.037499999999998	29.1375	25.9875	22.8375
100-101	22.975	28.6125	26.625	21.7875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	2.0
25	3.5
26	3.5
27	3.0
28	4.0
29	10.5
30	32.0
31	77.5
32	122.5
33	110.5
34	95.0
35	97.5
36	93.0
37	93.5
38	87.5
39	86.5
40	99.0
41	144.0
42	194.5
43	204.0
44	217.5
45	234.0
46	239.0
47	252.5
48	226.0
49	166.0
50	131.5
51	127.0
52	106.5
53	81.5
54	81.0
55	112.0
56	93.0
57	50.0
58	59.5
59	57.0
60	37.5
61	21.5
62	27.5
63	24.5
64	6.5
65	5.5
66	4.5
67	1.5
68	2.0
69	4.0
70	4.0
71	8.5
72	9.0
73	5.0
74	5.5
75	4.5
76	5.5
77	11.0
78	9.5
79	2.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85034013605441	36.3
2	17.596371882086167	19.400000000000002
3	6.984126984126984	11.55
4	3.4013605442176873	7.5
5	2.0408163265306123	5.625
6	1.2698412698412698	4.2
7	0.8163265306122449	3.15
8	0.6349206349206349	2.8000000000000003
9	0.36281179138321995	1.7999999999999998
>10	1.0430839002267573	7.675
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	34	0.8500000000000001	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	23	0.575	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	16	0.4	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	15	0.375	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	15	0.375	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	13	0.325	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	13	0.325	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	13	0.325	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	13	0.325	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	13	0.325	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	12	0.3	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	12	0.3	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	12	0.3	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	11	0.27499999999999997	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	11	0.27499999999999997	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	11	0.27499999999999997	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	10	0.25	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	10	0.25	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	10	0.25	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	10	0.25	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	10	0.25	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	10	0.25	No Hit
CCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACC	10	0.25	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	9	0.22499999999999998	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	9	0.22499999999999998	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	9	0.22499999999999998	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	9	0.22499999999999998	No Hit
ATCAGACACAAGCAGACTCTTTCCGTTAAATGCTGTCCATTGGTTGGACG	9	0.22499999999999998	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	9	0.22499999999999998	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	9	0.22499999999999998	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	9	0.22499999999999998	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	8	0.2	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	8	0.2	No Hit
TTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTAT	8	0.2	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	8	0.2	No Hit
GGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGG	8	0.2	No Hit
CTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCAT	8	0.2	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	8	0.2	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	8	0.2	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	8	0.2	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	8	0.2	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	8	0.2	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	8	0.2	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	8	0.2	No Hit
GTTAAGGTCATGAATGAAAAACTGCAGCACTGCATGGAACTAACAGACCT	8	0.2	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	7	0.17500000000000002	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	7	0.17500000000000002	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	7	0.17500000000000002	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	7	0.17500000000000002	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	7	0.17500000000000002	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	7	0.17500000000000002	No Hit
TAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTAC	7	0.17500000000000002	No Hit
CTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATG	7	0.17500000000000002	No Hit
CAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATT	7	0.17500000000000002	No Hit
CAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAA	7	0.17500000000000002	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	7	0.17500000000000002	No Hit
TTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCA	7	0.17500000000000002	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	7	0.17500000000000002	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	7	0.17500000000000002	No Hit
CTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAGGAA	7	0.17500000000000002	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	7	0.17500000000000002	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	7	0.17500000000000002	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	7	0.17500000000000002	No Hit
CATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACA	6	0.15	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	6	0.15	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	6	0.15	No Hit
ATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCAA	6	0.15	No Hit
GGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACAC	6	0.15	No Hit
GCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCC	6	0.15	No Hit
AGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACA	6	0.15	No Hit
GTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGAT	6	0.15	No Hit
CGCCAGTTGAGTGGCTTGTGGTGCCGGTGTGATGGCCGAGTCCCCGGCGT	6	0.15	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	6	0.15	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	6	0.15	No Hit
GACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTT	6	0.15	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	6	0.15	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	6	0.15	No Hit
GGCGGCCGGTGGCGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTC	6	0.15	No Hit
GTGGAAGTGACAAGCCTGCCTAGAGATGCAGCAAATATTTTGGTGATGGG	6	0.15	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	6	0.15	No Hit
GTCAAACTGTCTCATAAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGC	6	0.15	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	6	0.15	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	6	0.15	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	6	0.15	No Hit
CTGGGACAGAGCAAACCTGGAAGAGCTTTATGACAAGACCTGCCAGTTCC	6	0.15	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	6	0.15	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	6	0.15	No Hit
GTGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAG	6	0.15	No Hit
TTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGATAAA	6	0.15	No Hit
AGTCTATTCAGTCCATTCCAGAGGCATTAAAAGCTGGGAAGAAAGTCAAA	5	0.125	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	5	0.125	No Hit
CTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCT	5	0.125	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	5	0.125	No Hit
GAAGAACTCAACTACATAAAGACAGAGGGACAATCAAAACTGCACAGAGG	5	0.125	No Hit
GAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTAT	5	0.125	No Hit
TGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGT	5	0.125	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	5	0.125	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	5	0.125	No Hit
ATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCC	5	0.125	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	5	0.125	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	5	0.125	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	5	0.125	No Hit
AAAGTGTTGAAGACCAGCGAAACTTCACAAGATATCCAGAAGGTTTCTAG	5	0.125	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	5	0.125	No Hit
TTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAG	5	0.125	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	5	0.125	No Hit
CCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTGAGCTTCCTTTTTAGA	5	0.125	No Hit
GAAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGT	5	0.125	No Hit
GATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGG	5	0.125	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	5	0.125	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	5	0.125	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	5	0.125	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	5	0.125	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	5	0.125	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	5	0.125	No Hit
CTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGT	5	0.125	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	5	0.125	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	5	0.125	No Hit
CATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTC	5	0.125	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	5	0.125	No Hit
AGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGT	5	0.125	No Hit
CAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAG	5	0.125	No Hit
CCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGAC	5	0.125	No Hit
AGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCA	5	0.125	No Hit
CGCCATTCTAGAGAAATTTGCGTTCTCCAACGCTCTCTGCCTGTCAGTGA	5	0.125	No Hit
CAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
ATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTC	5	0.125	No Hit
GAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAA	5	0.125	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	5	0.125	No Hit
CTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGT	5	0.125	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	5	0.125	No Hit
AAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGC	5	0.125	No Hit
ATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTT	5	0.125	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.025	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.1	0.0	0.0	0.0	0.0
76-77	0.15	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.325	0.0	0.0	0.0	0.0
82-83	0.42500000000000004	0.0	0.0	0.0	0.0
84-85	0.7250000000000001	0.0	0.0	0.0	0.0
86-87	1.1125	0.0	0.0	0.0	0.0
88-89	1.3375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510870 spots for SRR24882430.sra
Written 3510870 spots for SRR24882430.sra
Read 3510875 spots for SRR24882430.sra
Written 3510875 spots for SRR24882430.sra
SRR ids: ['SRR24882430.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_35xihs2p
SRR24882430.sra spots: 28086965
blocks: [[1, 3510870], [3510871, 7021740], [7021741, 10532610], [10532611, 14043480], [14043481, 17554350], [17554351, 21065220], [21065221, 24576090], [24576091, 28086965]]
SRR24882430 file size 6780616
SRR24882430 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882430 SRR24882430_1.fastq SRR24882430_2.fastq
Input file:	SRR24882430_1.fastq
Paired file:	SRR24882430_2.fastq
trimmed:	SRR24882430-trimmed-pair1.fastq, SRR24882430-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 03:04:19 2026 >> started

Fri Jul  3 03:04:42 2026 >> done (23.457s)
28086965 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
28086965 (100.00%) read pairs available; of these:
  922071 ( 3.28%) trimmed read pairs available after processing
27164894 (96.72%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       2	  0.00%
 66	      28	  0.00%
 67	    1132	  0.00%
 68	    5558	  0.02%
 69	    6793	  0.02%
 70	    7843	  0.03%
 71	    8829	  0.03%
 72	    9654	  0.03%
 73	   10679	  0.04%
 74	   11475	  0.04%
 75	   12675	  0.05%
 76	   13853	  0.05%
 77	   14930	  0.05%
 78	   15833	  0.06%
 79	   17346	  0.06%
 80	   18271	  0.07%
 81	   20040	  0.07%
 82	   21579	  0.08%
 83	   22747	  0.08%
 84	   24601	  0.09%
 85	   26927	  0.10%
 86	   26988	  0.10%
 87	   29584	  0.11%
 88	   31605	  0.11%
 89	   32314	  0.12%
 90	   35406	  0.13%
 91	   38509	  0.14%
 92	   38857	  0.14%
 93	   41669	  0.15%
 94	   44000	  0.16%
 95	   45532	  0.16%
 96	   48451	  0.17%
 97	   52900	  0.19%
 98	   53930	  0.19%
 99	   58404	  0.21%
100	   73127	  0.26%
101	27164894	 96.72%
28086965 reads passed initial QC


criterion=sequence-density
sequence-density=1.48
sequence-density-rank=1
fanout-score=2.55
fanout-score-rank=30
prefix-density=1.65
prefix-fanout=2.3
sequence=GATGGAGAAGATG


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=35
fanout-score=27.04
fanout-score-rank=1
prefix-density=0.45
prefix-fanout=3.5
sequence=CAGGTGCAGCTGAGTGTCCTTGAGGATGCCTTGTGTGTTCCTATAGTTTCTCAGGGCCTCGGCAGCCATGCGGTTGGAGTGGTTCAGCTGGATTTCCATCTCGTTCAGGTCTCCCTCCA


criterion=sequence-density
sequence-density=1.51
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=39
prefix-density=1.51
prefix-fanout=2.0
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=34
fanout-score=31.56
fanout-score-rank=1
prefix-density=0.38
prefix-fanout=5.7
sequence=TGAAGAAGAACCTGGAGCAGACGG
SRR24882430 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 03:04:56
                             Started mapping on |	Jul 03 03:04:57
                                    Finished on |	Jul 03 03:34:46
       Mapping speed, Million of reads per hour |	56.52

                          Number of input reads |	28086965
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	24809193
                        Uniquely mapped reads % |	88.33%
                          Average mapped length |	200.57
                       Number of splices: Total |	19329455
            Number of splices: Annotated (sjdb) |	19187931
                       Number of splices: GT/AG |	19196488
                       Number of splices: GC/AG |	107112
                       Number of splices: AT/AC |	10666
               Number of splices: Non-canonical |	15189
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.70
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.53
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3271946
             % of reads mapped to multiple loci |	11.65%
        Number of reads mapped to too many loci |	3319
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5826	5826	5826
N_multimapping	3271946	3271946	3271946
N_noFeature	1285578	22612295	1922812
N_ambiguous	1793454	355627	220306
UnstrandedReadsAssigned:21730161 PositiveStrandReadsAssigned:1841271 NegativeStrandReadsAssigned:22666075
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882430 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882430-trimmed-pair1.fastq
                             SRR24882430-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,086,965 reads, 24,829,850 reads pseudoaligned
[quant] estimated average fragment length: 180.366
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,342 rounds

 109283 SRR24882430.ke.tsv
  52636 SRR24882430.se.tsv
 161919 total
==> SRR24882430.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	11	0	0
ENSMUST00000196221.1	9	5.5	0	0
ENSMUST00000179664.1	11	7.5	0	0
ENSMUST00000178537.1	12	8.5	0	0
ENSMUST00000178862.1	14	10.5	0	0
ENSMUST00000179520.1	11	7.5	0	0
ENSMUST00000179883.1	16	11	0	0
ENSMUST00000195858.1	10	6.5	0	0
ENSMUST00000179932.1	12	8.5	0	0

==> SRR24882430.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	1
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882430 completed mapping pipeline successfully
