Starting /dee2/code/volunteer_pipeline.sh SRR24882422
    current disk space = 1376206644
    free memory = 198031288 
SRR24882422 SRAfilesize
c1c6c4afcee1413c31aa4199f50d1c56  SRR24882422.sra
SRR24882422.sra file validated
SRR24882422 is paired end
SRR24882422 is conventional basespace
SRR24882422 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.505	37.0	37.0	37.0	37.0	37.0
2	36.32575	37.0	37.0	37.0	37.0	37.0
3	36.5385	37.0	37.0	37.0	37.0	37.0
4	36.633	37.0	37.0	37.0	37.0	37.0
5	36.555	37.0	37.0	37.0	37.0	37.0
6	36.6505	37.0	37.0	37.0	37.0	37.0
7	36.512	37.0	37.0	37.0	37.0	37.0
8	36.5985	37.0	37.0	37.0	37.0	37.0
9	36.62	37.0	37.0	37.0	37.0	37.0
10-11	36.577749999999995	37.0	37.0	37.0	37.0	37.0
12-13	36.5705	37.0	37.0	37.0	37.0	37.0
14-15	36.5325	37.0	37.0	37.0	37.0	37.0
16-17	36.5595	37.0	37.0	37.0	37.0	37.0
18-19	36.56	37.0	37.0	37.0	37.0	37.0
20-21	36.60175	37.0	37.0	37.0	37.0	37.0
22-23	36.6015	37.0	37.0	37.0	37.0	37.0
24-25	36.56	37.0	37.0	37.0	37.0	37.0
26-27	36.53225	37.0	37.0	37.0	37.0	37.0
28-29	36.46225	37.0	37.0	37.0	37.0	37.0
30-31	36.5125	37.0	37.0	37.0	37.0	37.0
32-33	36.508250000000004	37.0	37.0	37.0	37.0	37.0
34-35	36.47	37.0	37.0	37.0	37.0	37.0
36-37	36.5325	37.0	37.0	37.0	37.0	37.0
38-39	36.462	37.0	37.0	37.0	37.0	37.0
40-41	36.507000000000005	37.0	37.0	37.0	37.0	37.0
42-43	36.486999999999995	37.0	37.0	37.0	37.0	37.0
44-45	36.486000000000004	37.0	37.0	37.0	37.0	37.0
46-47	36.49975	37.0	37.0	37.0	37.0	37.0
48-49	36.47825	37.0	37.0	37.0	37.0	37.0
50-51	36.43025	37.0	37.0	37.0	37.0	37.0
52-53	36.4715	37.0	37.0	37.0	37.0	37.0
54-55	36.483	37.0	37.0	37.0	37.0	37.0
56-57	36.40625	37.0	37.0	37.0	37.0	37.0
58-59	36.435	37.0	37.0	37.0	37.0	37.0
60-61	36.38825	37.0	37.0	37.0	37.0	37.0
62-63	36.38475	37.0	37.0	37.0	37.0	37.0
64-65	36.412	37.0	37.0	37.0	37.0	37.0
66-67	36.3785	37.0	37.0	37.0	37.0	37.0
68-69	36.37375	37.0	37.0	37.0	37.0	37.0
70-71	36.37625	37.0	37.0	37.0	37.0	37.0
72-73	36.363749999999996	37.0	37.0	37.0	37.0	37.0
74-75	36.4025	37.0	37.0	37.0	37.0	37.0
76-77	36.32925	37.0	37.0	37.0	37.0	37.0
78-79	36.282	37.0	37.0	37.0	37.0	37.0
80-81	36.34025	37.0	37.0	37.0	37.0	37.0
82-83	36.34925	37.0	37.0	37.0	37.0	37.0
84-85	36.267250000000004	37.0	37.0	37.0	37.0	37.0
86-87	36.247	37.0	37.0	37.0	37.0	37.0
88-89	36.233	37.0	37.0	37.0	37.0	37.0
90-91	36.22825	37.0	37.0	37.0	37.0	37.0
92-93	36.20025	37.0	37.0	37.0	37.0	37.0
94-95	36.1555	37.0	37.0	37.0	37.0	37.0
96-97	36.14675	37.0	37.0	37.0	37.0	37.0
98-99	36.165625000000006	37.0	37.0	37.0	37.0	37.0
100-101	35.9435	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	1.0
26	2.0
27	1.0
28	6.0
29	10.0
30	16.0
31	28.0
32	43.0
33	46.0
34	98.0
35	270.0
36	2680.0
37	798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.925	8.6	13.025	39.45
2	17.81336002001501	12.909682261696274	38.20365273955467	31.07330497873405
3	18.91891891891892	16.29129129129129	32.85785785785786	31.931931931931935
4	24.55	25.025	25.074999999999996	25.35
5	24.3	27.250000000000004	26.35	22.1
6	21.45	30.025000000000002	29.9	18.625
7	17.25	21.099999999999998	46.525	15.125
8	19.575	21.825	35.825	22.775000000000002
9	20.625	22.0	35.675000000000004	21.7
10-11	21.4375	30.775000000000002	26.5625	21.224999999999998
12-13	21.575	24.4125	29.9375	24.075
14-15	20.962500000000002	26.437500000000004	31.35	21.25
16-17	21.3125	27.875	27.8125	23.0
18-19	23.549999999999997	27.6	26.8125	22.037499999999998
20-21	22.1875	25.937500000000004	29.012500000000003	22.8625
22-23	21.85	26.5375	27.3125	24.3
24-25	22.787499999999998	27.3	26.387500000000003	23.525
26-27	21.625	28.537499999999998	28.1125	21.725
28-29	21.075	27.462500000000002	29.575000000000003	21.8875
30-31	21.3875	25.650000000000002	29.6625	23.3
32-33	21.55	24.975	31.125000000000004	22.35
34-35	20.3	27.287499999999998	27.462500000000002	24.95
36-37	21.55	26.200000000000003	31.112499999999997	21.1375
38-39	21.2875	27.800000000000004	30.4375	20.474999999999998
40-41	21.8125	27.787499999999998	29.9375	20.4625
42-43	21.575	29.349999999999998	28.962500000000002	20.1125
44-45	20.549999999999997	28.287499999999998	28.599999999999998	22.5625
46-47	22.425	28.787499999999998	26.0125	22.775000000000002
48-49	21.15	28.787499999999998	27.4125	22.650000000000002
50-51	22.625	26.674999999999997	27.787499999999998	22.912499999999998
52-53	20.525	25.825	30.0875	23.5625
54-55	21.1875	25.6125	29.125	24.075
56-57	18.712500000000002	28.5625	29.15	23.575
58-59	20.45	25.7	29.062500000000004	24.7875
60-61	20.4875	28.249999999999996	28.7375	22.525000000000002
62-63	22.975	27.375	26.7125	22.9375
64-65	22.9875	24.1625	29.7875	23.0625
66-67	23.849999999999998	25.2875	29.1375	21.725
68-69	22.25	27.5125	26.787499999999998	23.45
70-71	22.0625	28.175	27.275	22.4875
72-73	21.224999999999998	29.299999999999997	26.7125	22.7625
74-75	21.075	28.462500000000002	28.525	21.9375
76-77	23.525	28.275	27.150000000000002	21.05
78-79	23.2125	26.25	27.0875	23.45
80-81	23.150000000000002	27.200000000000003	27.625	22.025
82-83	24.2375	27.8875	27.525	20.349999999999998
84-85	21.325	28.5875	26.450000000000003	23.6375
86-87	22.8375	28.075	27.075	22.0125
88-89	23.7625	27.500000000000004	27.487499999999997	21.25
90-91	21.475	26.0625	28.8625	23.599999999999998
92-93	21.075	27.250000000000004	27.725	23.95
94-95	21.975	27.462500000000002	28.7	21.8625
96-97	19.85	27.1125	27.0625	25.974999999999998
98-99	21.190148768596075	28.091011376422053	28.678584823102888	22.040255031878985
100-101	21.725	27.5125	28.4	22.3625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	2.5
22	5.0
23	3.5
24	3.5
25	5.5
26	6.0
27	6.5
28	10.0
29	18.5
30	45.0
31	65.0
32	103.0
33	121.5
34	105.0
35	121.0
36	114.5
37	98.5
38	82.5
39	66.5
40	79.0
41	137.0
42	201.0
43	180.5
44	143.0
45	165.5
46	238.0
47	276.5
48	225.5
49	190.5
50	176.5
51	147.0
52	123.0
53	118.0
54	111.5
55	77.5
56	67.0
57	63.0
58	60.5
59	78.0
60	63.5
61	31.5
62	20.0
63	11.0
64	2.0
65	1.0
66	2.0
67	4.5
68	3.5
69	3.0
70	4.0
71	2.0
72	1.5
73	1.5
74	0.5
75	0.0
76	0.0
77	0.5
78	0.5
79	1.5
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.075
3	0.1
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0125
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.93285371702638	33.324999999999996
2	18.94484412470024	19.75
3	7.194244604316546	11.25
4	2.6378896882494005	5.5
5	2.302158273381295	6.0
6	1.2470023980815348	3.9
7	0.815347721822542	2.9749999999999996
8	0.671462829736211	2.8000000000000003
9	0.6235011990407674	2.9250000000000003
>10	1.630695443645084	11.575000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	27	0.675	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	27	0.675	No Hit
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	25	0.625	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	20	0.5	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	18	0.44999999999999996	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	18	0.44999999999999996	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	16	0.4	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	16	0.4	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	16	0.4	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	14	0.35000000000000003	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	14	0.35000000000000003	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	13	0.325	No Hit
GTCCCCTGCGCACTGACAGCCCCTTGACTTAGAGGGTAGAGTAAGAGTCA	13	0.325	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	13	0.325	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	13	0.325	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	12	0.3	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	12	0.3	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	12	0.3	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	11	0.27499999999999997	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	11	0.27499999999999997	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	11	0.27499999999999997	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	11	0.27499999999999997	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	10	0.25	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	10	0.25	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	10	0.25	No Hit
CTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAA	10	0.25	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	10	0.25	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	10	0.25	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	10	0.25	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	10	0.25	No Hit
CTCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCCA	10	0.25	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	10	0.25	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	10	0.25	No Hit
CCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAGTTGAGTTCT	10	0.25	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	9	0.22499999999999998	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	9	0.22499999999999998	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	9	0.22499999999999998	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	9	0.22499999999999998	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	9	0.22499999999999998	No Hit
GTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGA	9	0.22499999999999998	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	9	0.22499999999999998	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	9	0.22499999999999998	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	9	0.22499999999999998	No Hit
TCTGTCTTTATGTAGTTGAGTTCTTCGTTCTCCCAGTGAACGAGAGCCAC	9	0.22499999999999998	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	9	0.22499999999999998	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	9	0.22499999999999998	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	9	0.22499999999999998	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	8	0.2	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	8	0.2	No Hit
TATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTC	8	0.2	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	8	0.2	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	8	0.2	No Hit
GGGTCATGCTCCTGTGAGTCCCAAAGGGGTGCATGCTTGGCCAGTGTTCT	8	0.2	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	8	0.2	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	8	0.2	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	8	0.2	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	8	0.2	No Hit
TAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACA	8	0.2	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	8	0.2	No Hit
TTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGT	8	0.2	No Hit
CTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAATCAG	8	0.2	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	7	0.17500000000000002	No Hit
CTTTGCCATTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAA	7	0.17500000000000002	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	7	0.17500000000000002	No Hit
GTCCGGGATGCTTTCAGCGGCCTCTTGATCTGCGGAAGGTCTTGTTTGGG	7	0.17500000000000002	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	7	0.17500000000000002	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	7	0.17500000000000002	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	7	0.17500000000000002	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	7	0.17500000000000002	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	7	0.17500000000000002	No Hit
GTGCGTTTCCCTGTGTAGCCAGGGCTGCTCTGGACATCAGGATCTGCCTG	7	0.17500000000000002	No Hit
GCTTAATCTGACACTAAGGAGACTTCTTTCCAACCTGGACTGGATTAGAA	7	0.17500000000000002	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	7	0.17500000000000002	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	7	0.17500000000000002	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	7	0.17500000000000002	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	7	0.17500000000000002	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	7	0.17500000000000002	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	7	0.17500000000000002	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	6	0.15	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	6	0.15	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	6	0.15	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	6	0.15	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	6	0.15	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	6	0.15	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	6	0.15	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	6	0.15	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	6	0.15	No Hit
GCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTTT	6	0.15	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	6	0.15	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	6	0.15	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	6	0.15	No Hit
TGTAAATGTTGCTGTATTGTCAGGCCATTGTGGCATGTTGTACTTTCAGA	6	0.15	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	6	0.15	No Hit
CCTTCTTTTGCAGAACTTTCCACACCCATCACCAAAATATTTGCTGCATC	6	0.15	No Hit
CTCTTTACTGCTTTTATAAACCACTTAGAACACAAGAGATTTGGTTTGTG	6	0.15	No Hit
CTGGAATGGACTGAATAGACTCAATAAATTTGTCCAGGGTTGCTTCCCAA	6	0.15	No Hit
GCCACTTCATAGGGCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTG	6	0.15	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	6	0.15	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	6	0.15	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	6	0.15	No Hit
CCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCA	6	0.15	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	6	0.15	No Hit
CCGCATTAGGTCTGTTAGTTCCATGCAGTGCTGCAGTTTTTCATTCATGA	6	0.15	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	6	0.15	No Hit
AGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATC	5	0.125	No Hit
GTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATG	5	0.125	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	5	0.125	No Hit
GGGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATT	5	0.125	No Hit
GTTTCGGGGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGAT	5	0.125	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	5	0.125	No Hit
CTTCGCGTGATGCTAAGGAACTGGCAGGTCTTGTCATAAAGCTCTTCCAG	5	0.125	No Hit
CTTAACTCTTCGCGTGATGCTAAGGAACTGGCAGGTCTTGTCATAAAGCT	5	0.125	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	5	0.125	No Hit
CAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGGTGATCAAGAAATCTGAGC	5	0.125	No Hit
TCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCATTAAAATATT	5	0.125	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	5	0.125	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	5	0.125	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	5	0.125	No Hit
CACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	5	0.125	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	5	0.125	No Hit
CTTCCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGGTGATCAAGAAATCT	5	0.125	No Hit
GCTTTCATGATCACAAGGTCAGAAGAAGACTCTCCCCAGCTCAAACATTA	5	0.125	No Hit
GGTAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	5	0.125	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	5	0.125	No Hit
GACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAA	5	0.125	No Hit
GCTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAA	5	0.125	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	5	0.125	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	5	0.125	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	5	0.125	No Hit
GCCCTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCC	5	0.125	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	5	0.125	No Hit
CCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGTTG	5	0.125	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	5	0.125	No Hit
GTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTG	5	0.125	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	5	0.125	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	5	0.125	No Hit
CCCATCACCAAAATATTTGCTGCATCTCTAGGCAGGCTTGTCACTTCCAC	5	0.125	No Hit
CACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTT	5	0.125	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	5	0.125	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	5	0.125	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	5	0.125	No Hit
CAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGAT	5	0.125	No Hit
GCTGTATTGTCAGGCCATTGTGGCATGTTGTACTTTCAGATTTCCTAAGT	5	0.125	No Hit
TTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTT	5	0.125	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	5	0.125	No Hit
TGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCT	5	0.125	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	5	0.125	No Hit
CTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCC	5	0.125	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	5	0.125	No Hit
GGCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTT	5	0.125	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	5	0.125	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.0875	0.0	0.0	0.0	0.0
74-75	0.2	0.0	0.0	0.0	0.0
76-77	0.325	0.0	0.0	0.0	0.0
78-79	0.4375	0.0	0.0	0.0	0.0
80-81	0.475	0.0	0.0	0.0	0.0
82-83	0.5375000000000001	0.0	0.0	0.0	0.0
84-85	0.7124999999999999	0.0	0.0	0.0	0.0
86-87	0.8125	0.0	0.0	0.0	0.0
88-89	0.9125	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882422 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882422_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.379	37.0	37.0	37.0	37.0	37.0
2	36.218	37.0	37.0	37.0	37.0	37.0
3	36.3405	37.0	37.0	37.0	37.0	37.0
4	36.347	37.0	37.0	37.0	37.0	37.0
5	36.436	37.0	37.0	37.0	37.0	37.0
6	36.271	37.0	37.0	37.0	37.0	37.0
7	36.40725	37.0	37.0	37.0	37.0	37.0
8	36.4515	37.0	37.0	37.0	37.0	37.0
9	36.402	37.0	37.0	37.0	37.0	37.0
10-11	36.439	37.0	37.0	37.0	37.0	37.0
12-13	36.44425	37.0	37.0	37.0	37.0	37.0
14-15	36.4075	37.0	37.0	37.0	37.0	37.0
16-17	36.3765	37.0	37.0	37.0	37.0	37.0
18-19	36.31575	37.0	37.0	37.0	37.0	37.0
20-21	36.2965	37.0	37.0	37.0	37.0	37.0
22-23	36.3235	37.0	37.0	37.0	37.0	37.0
24-25	36.345	37.0	37.0	37.0	37.0	37.0
26-27	36.32625	37.0	37.0	37.0	37.0	37.0
28-29	36.28275	37.0	37.0	37.0	37.0	37.0
30-31	36.248999999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.253	37.0	37.0	37.0	37.0	37.0
34-35	36.24675	37.0	37.0	37.0	37.0	37.0
36-37	36.27675000000001	37.0	37.0	37.0	37.0	37.0
38-39	36.210499999999996	37.0	37.0	37.0	37.0	37.0
40-41	36.247	37.0	37.0	37.0	37.0	37.0
42-43	36.16375	37.0	37.0	37.0	37.0	37.0
44-45	36.076499999999996	37.0	37.0	37.0	37.0	37.0
46-47	36.14875	37.0	37.0	37.0	37.0	37.0
48-49	36.046	37.0	37.0	37.0	37.0	37.0
50-51	36.05925	37.0	37.0	37.0	37.0	37.0
52-53	36.094750000000005	37.0	37.0	37.0	37.0	37.0
54-55	36.0315	37.0	37.0	37.0	37.0	37.0
56-57	36.1455	37.0	37.0	37.0	37.0	37.0
58-59	36.001875	37.0	37.0	37.0	37.0	37.0
60-61	36.015	37.0	37.0	37.0	37.0	37.0
62-63	36.0445	37.0	37.0	37.0	37.0	37.0
64-65	35.91825	37.0	37.0	37.0	37.0	37.0
66-67	36.042	37.0	37.0	37.0	37.0	37.0
68-69	35.986000000000004	37.0	37.0	37.0	37.0	37.0
70-71	36.002250000000004	37.0	37.0	37.0	37.0	37.0
72-73	35.81125	37.0	37.0	37.0	37.0	37.0
74-75	36.0035	37.0	37.0	37.0	37.0	37.0
76-77	35.862750000000005	37.0	37.0	37.0	37.0	37.0
78-79	35.802	37.0	37.0	37.0	37.0	37.0
80-81	35.81	37.0	37.0	37.0	37.0	37.0
82-83	35.73475	37.0	37.0	37.0	37.0	37.0
84-85	35.8345	37.0	37.0	37.0	37.0	37.0
86-87	35.829499999999996	37.0	37.0	37.0	37.0	37.0
88-89	35.71075	37.0	37.0	37.0	37.0	37.0
90-91	35.744	37.0	37.0	37.0	37.0	37.0
92-93	35.746125	37.0	37.0	37.0	37.0	37.0
94-95	35.66025	37.0	37.0	37.0	37.0	37.0
96-97	35.72525	37.0	37.0	37.0	37.0	37.0
98-99	35.6205	37.0	37.0	37.0	37.0	37.0
100-101	35.45625	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	6.0
25	1.0
26	9.0
27	12.0
28	13.0
29	17.0
30	22.0
31	26.0
32	49.0
33	80.0
34	165.0
35	513.0
36	2778.0
37	308.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.253132832080205	18.596491228070175	14.987468671679197	25.162907268170425
2	20.724999999999998	28.1	33.125	18.05
3	21.224999999999998	27.474999999999998	30.65	20.65
4	26.0	34.699999999999996	21.625	17.675
5	25.674999999999997	33.550000000000004	22.825	17.95
6	22.1	37.175000000000004	23.525	17.2
7	20.38009502375594	21.780445111277817	37.034258564641156	20.80520130032508
8	22.475	24.125	30.375000000000004	23.025000000000002
9	23.45	23.575	28.599999999999998	24.375
10-11	23.6875	33.6125	21.925	20.775
12-13	24.7	25.825	25.2	24.275
14-15	24.375	26.724999999999998	28.037499999999998	20.8625
16-17	24.425	28.0875	27.474999999999998	20.0125
18-19	22.425	28.525	27.487499999999997	21.5625
20-21	23.2625	28.575	28.15	20.0125
22-23	23.5125	28.425	25.7	22.3625
24-25	25.45	27.6375	25.2875	21.625
26-27	22.287499999999998	28.95	26.6	22.162499999999998
28-29	23.775	28.425	26.575	21.224999999999998
30-31	24.0125	27.900000000000002	26.6	21.4875
32-33	23.9875	27.900000000000002	27.375	20.7375
34-35	24.3125	28.262500000000003	25.825	21.6
36-37	22.975	28.050000000000004	28.675	20.3
38-39	22.9875	28.475	26.387500000000003	22.15
40-41	22.15	29.225	27.0125	21.6125
42-43	23.175	28.825	26.5	21.5
44-45	23.425	27.5875	27.875	21.1125
46-47	23.45	28.462500000000002	26.1125	21.975
48-49	23.3375	27.5625	27.2625	21.837500000000002
50-51	23.9375	28.1625	26.437500000000004	21.462500000000002
52-53	23.474999999999998	27.6125	27.987499999999997	20.925
54-55	24.637500000000003	27.6	26.075	21.6875
56-57	21.6625	29.262500000000003	25.837500000000002	23.2375
58-59	22.75284410551319	29.47868483560445	25.828228528566072	21.94024253031629
60-61	22.575	28.9	26.3125	22.2125
62-63	23.0125	28.549999999999997	26.1625	22.275
64-65	24.075	27.8875	28.1625	19.875
66-67	22.15	27.537499999999998	28.262500000000003	22.05
68-69	22.45	29.012500000000003	26.0625	22.475
70-71	22.0	29.312500000000004	27.625	21.0625
72-73	23.0125	28.4	26.650000000000002	21.9375
74-75	23.35	28.549999999999997	27.35	20.75
76-77	22.8875	29.362500000000004	26.8125	20.9375
78-79	24.3	28.8875	25.624999999999996	21.1875
80-81	22.8125	28.075	28.3625	20.75
82-83	23.2875	27.250000000000004	28.287499999999998	21.175
84-85	21.4875	28.225	27.3	22.9875
86-87	22.0125	29.262500000000003	26.7125	22.0125
88-89	22.525000000000002	28.6625	27.237499999999997	21.575
90-91	24.75	27.6125	25.4625	22.175
92-93	23.40292536567071	29.716214526815854	27.128391048881113	19.75246905863233
94-95	23.125	30.0	25.912499999999998	20.962500000000002
96-97	23.625	28.7	25.6125	22.0625
98-99	23.4875	29.1125	25.662499999999998	21.7375
100-101	24.15	28.025	26.8	21.025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	2.0
22	2.0
23	2.5
24	3.0
25	1.5
26	4.5
27	6.0
28	7.5
29	11.0
30	32.5
31	83.5
32	128.5
33	116.0
34	92.5
35	91.0
36	98.0
37	114.5
38	97.5
39	92.0
40	113.0
41	139.5
42	199.0
43	204.5
44	187.0
45	212.0
46	214.5
47	221.5
48	213.0
49	171.0
50	158.0
51	156.5
52	130.0
53	85.5
54	70.0
55	94.0
56	77.0
57	50.0
58	61.0
59	55.0
60	31.0
61	21.0
62	22.5
63	18.0
64	9.0
65	8.5
66	6.5
67	2.0
68	2.5
69	6.0
70	6.0
71	6.0
72	7.5
73	4.5
74	6.0
75	9.0
76	12.5
77	13.5
78	6.0
79	0.5
80	0.0
81	0.5
82	0.5
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0125
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.900000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.80322003577818	36.225
2	18.783542039355993	21.0
3	7.4239713774597496	12.45
4	3.041144901610018	6.800000000000001
5	2.012522361359571	5.625
6	1.4311270125223614	4.8
7	0.7155635062611807	2.8000000000000003
8	0.5813953488372093	2.6
9	0.35778175313059035	1.7999999999999998
>10	0.8497316636851521	5.8999999999999995
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	21	0.525	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	15	0.375	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	15	0.375	No Hit
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	14	0.35000000000000003	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	14	0.35000000000000003	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	13	0.325	No Hit
AGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTA	13	0.325	No Hit
GGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGG	12	0.3	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	12	0.3	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	12	0.3	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	12	0.3	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	12	0.3	No Hit
GTGGAAGTGACAAGCCTGCCTAGAGATGCAGCAAATATTTTGGTGATGGG	11	0.27499999999999997	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	10	0.25	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	10	0.25	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	10	0.25	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	10	0.25	No Hit
GTGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAG	10	0.25	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	10	0.25	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	9	0.22499999999999998	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	9	0.22499999999999998	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	9	0.22499999999999998	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	9	0.22499999999999998	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	9	0.22499999999999998	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	9	0.22499999999999998	No Hit
GCCAGAGATCCTGGAAATGAGCCGGATAACATCAGACACAAGCAGACTCT	9	0.22499999999999998	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	9	0.22499999999999998	No Hit
GGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAG	8	0.2	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	8	0.2	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	8	0.2	No Hit
GTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAG	8	0.2	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	8	0.2	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	8	0.2	No Hit
CATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAA	8	0.2	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	8	0.2	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	8	0.2	No Hit
GAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGG	8	0.2	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	8	0.2	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	8	0.2	No Hit
AATAAACCTGAGCTCAGATTTCTTGATCACCCCTGACTTCTACTGGGACA	8	0.2	No Hit
ATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAA	7	0.17500000000000002	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	7	0.17500000000000002	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	7	0.17500000000000002	No Hit
ACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTC	7	0.17500000000000002	No Hit
CTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAG	7	0.17500000000000002	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	7	0.17500000000000002	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	7	0.17500000000000002	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	7	0.17500000000000002	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	7	0.17500000000000002	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	7	0.17500000000000002	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	7	0.17500000000000002	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	7	0.17500000000000002	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	7	0.17500000000000002	No Hit
CTGGGACAGAGCAAACCTGGAAGAGCTTTATGACAAGACCTGCCAGTTCC	7	0.17500000000000002	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	7	0.17500000000000002	No Hit
GTTTGAATAAGCCAGAGATCCTGGAAATGAGCCGGATAACATCAGACACA	7	0.17500000000000002	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	6	0.15	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	6	0.15	No Hit
CTGGAAATGAGCCGGATAACATCAGACACAAGCAGACTCTTTCCGTTAAA	6	0.15	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	6	0.15	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	6	0.15	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	6	0.15	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	6	0.15	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	6	0.15	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	6	0.15	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	6	0.15	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	6	0.15	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	6	0.15	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	6	0.15	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	6	0.15	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	6	0.15	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	6	0.15	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	6	0.15	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	6	0.15	No Hit
CAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAA	6	0.15	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	6	0.15	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	6	0.15	No Hit
GAAAAAACTATGAAGCACGTGATGCAAGTTCTAGAGAGACACGAAACCCA	6	0.15	No Hit
GGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTG	6	0.15	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	6	0.15	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	6	0.15	No Hit
CAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAG	6	0.15	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	6	0.15	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	6	0.15	No Hit
ATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTCC	6	0.15	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	6	0.15	No Hit
GAGGACCTGATGCAGTGCACAGCATTTGCAACCGCAGATGAGTATCACCT	6	0.15	No Hit
GCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATA	6	0.15	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	5	0.125	No Hit
CCGGAACAATATTCTTGTTCAGGGAAGGAGCTGCTGTGTTCTGGAATGTA	5	0.125	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	5	0.125	No Hit
AAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTG	5	0.125	No Hit
ATCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAA	5	0.125	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	5	0.125	No Hit
AGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCT	5	0.125	No Hit
GCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAG	5	0.125	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	5	0.125	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	5	0.125	No Hit
ATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTCATGTACTTATTG	5	0.125	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	5	0.125	No Hit
TGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATT	5	0.125	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	5	0.125	No Hit
CTCAGGCTCTGGCGCGCGGGGACATGTCGGTGGTGACTGGCGGCGGTGAG	5	0.125	No Hit
TGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATG	5	0.125	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	5	0.125	No Hit
TTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACA	5	0.125	No Hit
GGCCGCTGAAAGCATCCCGGACCCGGCAGCCATCCAGGACCAACCTTCCA	5	0.125	No Hit
GATGAGTATCACCTGGGAAGTCTGTCTCAAGAGCTGGTCTCCTGTGGCTA	5	0.125	No Hit
GAGCTTTATGACAAGACCTGCCAGTTCCTTAGCATCACGCGAAGAGTTAA	5	0.125	No Hit
TACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTT	5	0.125	No Hit
GGATGATCGTCATCCTCATCACCATTGAGGTAATGTTTGAGCTGGGGAGA	5	0.125	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	5	0.125	No Hit
TTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTG	5	0.125	No Hit
CGTGTCTTCTTCCAGAGTCCCCGCGGTGGAACCGGCGGCAGCCCAGGATC	5	0.125	No Hit
CCCAAACAAGACCTTCCGCAGATCAAGAGGCCGCTGAAAGCATCCCGGAC	5	0.125	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	5	0.125	No Hit
AGGAGTTTGAGGCCAGCCTGGGCAAGACCTTGTCCAAAAAATAAAAAAAT	5	0.125	No Hit
TTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCA	5	0.125	No Hit
TGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAAT	5	0.125	No Hit
AGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGC	5	0.125	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	5	0.125	No Hit
TATATGTATACTTGAAACATATATTTAAATCCAAGCTGACTTCTTAATTC	5	0.125	No Hit
GAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGA	5	0.125	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	5	0.125	No Hit
GCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTAAAAT	5	0.125	No Hit
CTAACAGACCTAATGCGGAATCACCTCAATGAGAAGCGAGCACTCCGCTT	5	0.125	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	5	0.125	No Hit
GGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGAC	5	0.125	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	5	0.125	No Hit
AAGAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGC	5	0.125	No Hit
GTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATG	5	0.125	No Hit
TATCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTT	5	0.125	No Hit
CAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.05	0.0	0.0	0.0	0.0
72-73	0.0875	0.0	0.0	0.0	0.0
74-75	0.2	0.0	0.0	0.0	0.0
76-77	0.325	0.0	0.0	0.0	0.0
78-79	0.44999999999999996	0.0	0.0	0.0	0.0
80-81	0.5	0.0	0.0	0.0	0.0
82-83	0.55	0.0	0.0	0.0	0.0
84-85	0.7124999999999999	0.0	0.0	0.0	0.0
86-87	0.8125	0.0	0.0	0.0	0.0
88-89	0.9125	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750353 spots for SRR24882422.sra
Written 3750353 spots for SRR24882422.sra
Read 3750354 spots for SRR24882422.sra
Written 3750354 spots for SRR24882422.sra
SRR ids: ['SRR24882422.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_616264eh
SRR24882422.sra spots: 30002825
blocks: [[1, 3750353], [3750354, 7500706], [7500707, 11251059], [11251060, 15001412], [15001413, 18751765], [18751766, 22502118], [22502119, 26252471], [26252472, 30002825]]
SRR24882422 file size 7244612
SRR24882422 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882422 SRR24882422_1.fastq SRR24882422_2.fastq
Input file:	SRR24882422_1.fastq
Paired file:	SRR24882422_2.fastq
trimmed:	SRR24882422-trimmed-pair1.fastq, SRR24882422-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 02:09:54 2026 >> started

Fri Jul  3 02:10:22 2026 >> done (27.837s)
30002825 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
30002825 (100.00%) read pairs available; of these:
  762163 ( 2.54%) trimmed read pairs available after processing
29240662 (97.46%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 64	       1	  0.00%
 65	       5	  0.00%
 66	      25	  0.00%
 67	    1753	  0.01%
 68	    9586	  0.03%
 69	   11293	  0.04%
 70	   12547	  0.04%
 71	   13364	  0.04%
 72	   14471	  0.05%
 73	   14665	  0.05%
 74	   14865	  0.05%
 75	   16216	  0.05%
 76	   17135	  0.06%
 77	   17159	  0.06%
 78	   17407	  0.06%
 79	   18336	  0.06%
 80	   19044	  0.06%
 81	   19675	  0.07%
 82	   20484	  0.07%
 83	   20846	  0.07%
 84	   22287	  0.07%
 85	   22733	  0.08%
 86	   22681	  0.08%
 87	   23903	  0.08%
 88	   24755	  0.08%
 89	   24351	  0.08%
 90	   26553	  0.09%
 91	   27082	  0.09%
 92	   27014	  0.09%
 93	   28543	  0.10%
 94	   29113	  0.10%
 95	   29109	  0.10%
 96	   30430	  0.10%
 97	   32496	  0.11%
 98	   32049	  0.11%
 99	   34354	  0.11%
100	   65833	  0.22%
101	29240662	 97.46%
30002825 reads passed initial QC


criterion=sequence-density
sequence-density=0.67
sequence-density-rank=1
fanout-score=9.19
fanout-score-rank=14
prefix-density=2.05
prefix-fanout=3.0
sequence=TGGAGAAGATGCCCATCGGCTTCTCGATGAGCTCGATGCAGGCCGCCAGGTCCATCCCGAAGTCAATGAACTCCCAATCAATGCCCTCCTTCTTGTACTCCTCCTGCTCCAGCACGAACATGTGGTGGTTGAAAAACTGTTGCAGTTTCTCATTGGTGAAGTTGATGCACAGCTGCTCCAAGGTGTTGAAGTCAAAGATCTCAAAGCCAGCAATGTCCAAGACCCCGATGAAGTACTGCCGGGGCTGCTTGGTGTCCAGCTGCTGGTTGATGCGGGTGACCATCCATAGGAACATCTTCTCATACATGGACTTGGCCAGGGCACCCACGGAGTTGTACACCTGCTGCACCGTCTGGCCTTTGGTGACATACTCATTGCCGACCTTGACCCTGGGGTAGCAGAGGGCTTTGAGCAGGTCAGCAGAGTTCAGACTTGTCAGATAGGCAGCCTTGTCAGCAACTTCGGTGCCGTCTGGCTCAGCCTGCTCTTCCCTTTGCTTTTGCTTGAATTT


criterion=fanout-score
sequence-density=0.21
sequence-density-rank=23
fanout-score=47.24
fanout-score-rank=1
prefix-density=2.59
prefix-fanout=3.8
sequence=CTTCTTCTTGGCC


criterion=sequence-density
sequence-density=1.35
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=33
prefix-density=1.33
prefix-fanout=2.0
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.09
sequence-density-rank=33
fanout-score=27.76
fanout-score-rank=1
prefix-density=0.67
prefix-fanout=3.7
sequence=CAGAAGGAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATCACCCT
SRR24882422 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 02:10:39
                             Started mapping on |	Jul 03 02:10:39
                                    Finished on |	Jul 03 02:25:27
       Mapping speed, Million of reads per hour |	121.63

                          Number of input reads |	30002825
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	25544909
                        Uniquely mapped reads % |	85.14%
                          Average mapped length |	200.61
                       Number of splices: Total |	17554146
            Number of splices: Annotated (sjdb) |	17417440
                       Number of splices: GT/AG |	17422196
                       Number of splices: GC/AG |	105752
                       Number of splices: AT/AC |	10486
               Number of splices: Non-canonical |	15712
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.36
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.55
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4448794
             % of reads mapped to multiple loci |	14.83%
        Number of reads mapped to too many loci |	3610
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9122	9122	9122
N_multimapping	4448794	4448794	4448794
N_noFeature	1448645	23594568	1903183
N_ambiguous	1755102	318378	239901
UnstrandedReadsAssigned:22341162 PositiveStrandReadsAssigned:1631963 NegativeStrandReadsAssigned:23401825
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882422 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882422-trimmed-pair1.fastq
                             SRR24882422-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 30,002,825 reads, 26,335,233 reads pseudoaligned
[quant] estimated average fragment length: 224.282
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,271 rounds

 109283 SRR24882422.ke.tsv
  52636 SRR24882422.se.tsv
 161919 total
==> SRR24882422.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	10.5	0	0
ENSMUST00000196221.1	9	6	0	0
ENSMUST00000179664.1	11	8	0	0
ENSMUST00000178537.1	12	9	0	0
ENSMUST00000178862.1	14	8.5	0	0
ENSMUST00000179520.1	11	8	0	0
ENSMUST00000179883.1	16	10.5	0	0
ENSMUST00000195858.1	10	7	0	0
ENSMUST00000179932.1	12	9	0	0

==> SRR24882422.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882422 completed mapping pipeline successfully
