Starting /dee2/code/volunteer_pipeline.sh SRR24882418
    current disk space = 1384709764
    free memory = 197504432 
SRR24882418 SRAfilesize
61f154de6acb140b7a94603e07652ee5  SRR24882418.sra
SRR24882418.sra file validated
SRR24882418 is paired end
SRR24882418 is conventional basespace
SRR24882418 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6855	37.0	37.0	37.0	37.0	37.0
2	36.52	37.0	37.0	37.0	37.0	37.0
3	36.54175	37.0	37.0	37.0	37.0	37.0
4	36.5945	37.0	37.0	37.0	37.0	37.0
5	36.5765	37.0	37.0	37.0	37.0	37.0
6	36.46775	37.0	37.0	37.0	37.0	37.0
7	36.583	37.0	37.0	37.0	37.0	37.0
8	36.5435	37.0	37.0	37.0	37.0	37.0
9	36.544	37.0	37.0	37.0	37.0	37.0
10-11	36.59825	37.0	37.0	37.0	37.0	37.0
12-13	36.620999999999995	37.0	37.0	37.0	37.0	37.0
14-15	36.523250000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.608000000000004	37.0	37.0	37.0	37.0	37.0
18-19	36.52675	37.0	37.0	37.0	37.0	37.0
20-21	36.6285	37.0	37.0	37.0	37.0	37.0
22-23	36.556	37.0	37.0	37.0	37.0	37.0
24-25	36.5295	37.0	37.0	37.0	37.0	37.0
26-27	36.4495	37.0	37.0	37.0	37.0	37.0
28-29	36.5475	37.0	37.0	37.0	37.0	37.0
30-31	36.530249999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.471500000000006	37.0	37.0	37.0	37.0	37.0
34-35	36.556250000000006	37.0	37.0	37.0	37.0	37.0
36-37	36.472875	37.0	37.0	37.0	37.0	37.0
38-39	36.441	37.0	37.0	37.0	37.0	37.0
40-41	36.488	37.0	37.0	37.0	37.0	37.0
42-43	36.45975	37.0	37.0	37.0	37.0	37.0
44-45	36.49675	37.0	37.0	37.0	37.0	37.0
46-47	36.493	37.0	37.0	37.0	37.0	37.0
48-49	36.4135	37.0	37.0	37.0	37.0	37.0
50-51	36.516	37.0	37.0	37.0	37.0	37.0
52-53	36.453500000000005	37.0	37.0	37.0	37.0	37.0
54-55	36.34125	37.0	37.0	37.0	37.0	37.0
56-57	36.435249999999996	37.0	37.0	37.0	37.0	37.0
58-59	36.439	37.0	37.0	37.0	37.0	37.0
60-61	36.369	37.0	37.0	37.0	37.0	37.0
62-63	36.46275	37.0	37.0	37.0	37.0	37.0
64-65	36.352000000000004	37.0	37.0	37.0	37.0	37.0
66-67	36.39	37.0	37.0	37.0	37.0	37.0
68-69	36.36825	37.0	37.0	37.0	37.0	37.0
70-71	36.2505	37.0	37.0	37.0	37.0	37.0
72-73	36.35525	37.0	37.0	37.0	37.0	37.0
74-75	36.27275	37.0	37.0	37.0	37.0	37.0
76-77	36.283249999999995	37.0	37.0	37.0	37.0	37.0
78-79	36.241	37.0	37.0	37.0	37.0	37.0
80-81	36.235	37.0	37.0	37.0	37.0	37.0
82-83	36.313	37.0	37.0	37.0	37.0	37.0
84-85	36.22175	37.0	37.0	37.0	37.0	37.0
86-87	36.19375	37.0	37.0	37.0	37.0	37.0
88-89	36.25275	37.0	37.0	37.0	37.0	37.0
90-91	36.165499999999994	37.0	37.0	37.0	37.0	37.0
92-93	36.135999999999996	37.0	37.0	37.0	37.0	37.0
94-95	36.16675	37.0	37.0	37.0	37.0	37.0
96-97	36.0565	37.0	37.0	37.0	37.0	37.0
98-99	36.14275	37.0	37.0	37.0	37.0	37.0
100-101	35.912	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	2.0
27	2.0
28	8.0
29	16.0
30	20.0
31	17.0
32	34.0
33	56.0
34	94.0
35	278.0
36	2731.0
37	741.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.65	10.65	12.4	37.3
2	21.3	15.5	33.4	29.799999999999997
3	19.254813703425857	20.080020005001252	31.08277069267317	29.582395598899723
4	26.775	28.050000000000004	21.95	23.225
5	26.575	29.75	23.474999999999998	20.200000000000003
6	20.855213803450862	34.308577144286076	27.60690172543136	17.22930732683171
7	16.025	26.525	43.325	14.124999999999998
8	22.275	22.775000000000002	32.5	22.45
9	21.325	23.0	34.5	21.175
10-11	21.4875	34.025	24.15	20.3375
12-13	23.1375	26.187500000000004	28.799999999999997	21.875
14-15	20.95	30.225	29.299999999999997	19.525000000000002
16-17	21.099999999999998	28.575	27.725	22.6
18-19	22.787499999999998	29.625	26.35	21.2375
20-21	23.75	27.625	26.787499999999998	21.837500000000002
22-23	22.75	28.0875	25.974999999999998	23.1875
24-25	23.4375	28.487499999999997	26.3125	21.762500000000003
26-27	21.9375	30.375000000000004	28.225	19.4625
28-29	20.3125	29.5375	29.3375	20.8125
30-31	21.55	28.262500000000003	28.275	21.912499999999998
32-33	20.5	27.750000000000004	29.75	22.0
34-35	19.6875	28.1375	29.799999999999997	22.375
36-37	22.290286285785722	28.978622327790976	27.97849731216402	20.75259407425928
38-39	21.15	29.9375	29.275000000000002	19.6375
40-41	21.7	29.599999999999998	28.1375	20.5625
42-43	20.875	31.5	26.75	20.875
44-45	21.025	30.525000000000002	26.687499999999996	21.762500000000003
46-47	22.425	31.674999999999997	25.424999999999997	20.474999999999998
48-49	22.662499999999998	31.125000000000004	25.7875	20.424999999999997
50-51	22.037499999999998	28.425	27.487499999999997	22.05
52-53	21.475	26.674999999999997	28.65	23.200000000000003
54-55	21.2875	28.237499999999997	27.537499999999998	22.9375
56-57	19.8375	29.8875	27.8625	22.412499999999998
58-59	21.1125	28.975	27.150000000000002	22.7625
60-61	20.775	29.762499999999996	26.2875	23.175
62-63	23.25	28.599999999999998	27.224999999999998	20.925
64-65	22.4625	27.525	27.675	22.3375
66-67	24.5625	27.875	27.462500000000002	20.1
68-69	22.7125	29.1625	26.5625	21.5625
70-71	23.9	28.1625	27.474999999999998	20.4625
72-73	19.925	29.625	28.449999999999996	22.0
74-75	21.8875	29.5	27.8625	20.75
76-77	23.8625	28.849999999999998	26.525	20.7625
78-79	24.337500000000002	26.825	28.549999999999997	20.2875
80-81	24.962500000000002	28.025	26.674999999999997	20.3375
82-83	24.4375	29.362500000000004	26.3	19.900000000000002
84-85	21.512500000000003	30.9875	25.362499999999997	22.1375
86-87	20.674999999999997	30.2875	26.85	22.1875
88-89	24.25	29.9	27.575	18.275
90-91	23.5	29.1625	26.55	20.7875
92-93	21.75	28.249999999999996	27.85	22.15
94-95	21.1625	30.55	26.487500000000004	21.8
96-97	20.5625	30.4625	24.9125	24.0625
98-99	20.875	30.025000000000002	28.1125	20.9875
100-101	22.3125	29.9	27.175	20.6125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.5
20	0.5
21	4.0
22	7.0
23	5.5
24	5.0
25	15.0
26	23.0
27	14.0
28	8.0
29	19.5
30	42.0
31	75.5
32	126.5
33	148.5
34	133.0
35	153.5
36	153.5
37	125.5
38	90.0
39	67.0
40	108.0
41	184.5
42	229.5
43	193.0
44	147.0
45	145.5
46	193.5
47	215.5
48	182.0
49	133.5
50	119.5
51	121.0
52	90.5
53	66.5
54	62.0
55	71.5
56	76.5
57	58.0
58	59.0
59	103.0
60	90.5
61	38.5
62	23.5
63	12.5
64	5.0
65	4.5
66	1.0
67	2.0
68	5.0
69	3.5
70	3.5
71	4.5
72	2.5
73	5.0
74	5.0
75	5.0
76	5.5
77	2.0
78	1.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.025
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0125
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.83697813121272	34.625
2	14.66202783300199	14.75
3	5.2683896620278325	7.95
4	3.3300198807157058	6.7
5	2.286282306163022	5.75
6	1.4413518886679921	4.35
7	0.5964214711729622	2.1
8	0.5964214711729622	2.4
9	0.5467196819085487	2.475
>10	2.3856858846918487	17.625
>50	0.049701789264413515	1.275
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	51	1.275	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	31	0.775	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	28	0.7000000000000001	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	28	0.7000000000000001	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	26	0.65	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	24	0.6	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	23	0.575	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	21	0.525	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	21	0.525	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	17	0.42500000000000004	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	17	0.42500000000000004	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	16	0.4	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	16	0.4	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	16	0.4	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	15	0.375	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	15	0.375	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	15	0.375	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	15	0.375	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	14	0.35000000000000003	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	14	0.35000000000000003	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	14	0.35000000000000003	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	14	0.35000000000000003	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	14	0.35000000000000003	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	14	0.35000000000000003	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	14	0.35000000000000003	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	13	0.325	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	13	0.325	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	12	0.3	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	12	0.3	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	12	0.3	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	12	0.3	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	11	0.27499999999999997	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	11	0.27499999999999997	No Hit
ACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAA	11	0.27499999999999997	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	11	0.27499999999999997	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	11	0.27499999999999997	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	11	0.27499999999999997	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	11	0.27499999999999997	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	11	0.27499999999999997	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	11	0.27499999999999997	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	10	0.25	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	10	0.25	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	10	0.25	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	10	0.25	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	10	0.25	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	10	0.25	No Hit
GCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAA	10	0.25	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	10	0.25	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	10	0.25	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	9	0.22499999999999998	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	9	0.22499999999999998	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	9	0.22499999999999998	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	9	0.22499999999999998	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	9	0.22499999999999998	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	9	0.22499999999999998	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	9	0.22499999999999998	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	9	0.22499999999999998	No Hit
GGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	9	0.22499999999999998	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	9	0.22499999999999998	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	9	0.22499999999999998	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	8	0.2	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	8	0.2	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	8	0.2	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	8	0.2	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	8	0.2	No Hit
CACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGG	8	0.2	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	8	0.2	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	8	0.2	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	8	0.2	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	8	0.2	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	8	0.2	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	8	0.2	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	7	0.17500000000000002	No Hit
CAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATG	7	0.17500000000000002	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	7	0.17500000000000002	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	7	0.17500000000000002	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	7	0.17500000000000002	No Hit
CGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	7	0.17500000000000002	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	7	0.17500000000000002	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	7	0.17500000000000002	No Hit
TCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAAT	7	0.17500000000000002	No Hit
TTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTA	7	0.17500000000000002	No Hit
CTTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCC	7	0.17500000000000002	No Hit
CAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGT	7	0.17500000000000002	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	6	0.15	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	6	0.15	No Hit
AGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCT	6	0.15	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	6	0.15	No Hit
CCCATATGAATTTTAATTCTACTCAAAGAGCTGGTATGTAGCTGTAGGGT	6	0.15	No Hit
TGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCT	6	0.15	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	6	0.15	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	6	0.15	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	6	0.15	No Hit
CTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAAC	6	0.15	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	6	0.15	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	6	0.15	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	6	0.15	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	6	0.15	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	6	0.15	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	6	0.15	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	6	0.15	No Hit
GGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG	6	0.15	No Hit
GCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTT	6	0.15	No Hit
GTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACC	6	0.15	No Hit
CTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTT	6	0.15	No Hit
GTCCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCC	6	0.15	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	6	0.15	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	6	0.15	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	6	0.15	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	6	0.15	No Hit
CTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAA	6	0.15	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	6	0.15	No Hit
TTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGGCAATAA	5	0.125	No Hit
TGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCT	5	0.125	No Hit
GCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAAT	5	0.125	No Hit
GCTCCCTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATT	5	0.125	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	5	0.125	No Hit
CTTATTTCATGAACCTGATACAAAGCTCACATCTCTAGTTTTAACTTAGT	5	0.125	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	5	0.125	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	5	0.125	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	5	0.125	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	5	0.125	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	5	0.125	No Hit
AGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTA	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAAC	5	0.125	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	5	0.125	No Hit
GTCTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTT	5	0.125	No Hit
GGAAAATGTTGTGTGTGGGAGAATCAGCACAATGGCTCACGACGCTAATT	5	0.125	No Hit
GTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTC	5	0.125	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	5	0.125	No Hit
CCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCC	5	0.125	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	5	0.125	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	5	0.125	No Hit
TGTACTTTCCTATTTAATTGGTTATTGCTATTTAGCTACCGTTACTTATA	5	0.125	No Hit
CCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGC	5	0.125	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	5	0.125	No Hit
CCCGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	5	0.125	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	5	0.125	No Hit
CACATATGCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTCA	5	0.125	No Hit
GTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTG	5	0.125	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	5	0.125	No Hit
CCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAG	5	0.125	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	5	0.125	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	5	0.125	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	5	0.125	No Hit
GGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACC	5	0.125	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	5	0.125	No Hit
CCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCA	5	0.125	No Hit
CAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGAT	5	0.125	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	5	0.125	No Hit
GTTCAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATT	5	0.125	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	5	0.125	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	5	0.125	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	5	0.125	No Hit
CTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAA	5	0.125	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	5	0.125	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0625	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.15	0.0	0.0	0.0	0.0
76-77	0.175	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.2625	0.0	0.0	0.0	0.0
82-83	0.2875	0.0	0.0	0.0	0.0
84-85	0.375	0.0	0.0	0.0	0.0
86-87	0.5	0.0	0.0	0.0	0.0
88-89	0.6375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882418 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882418_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.42225	37.0	37.0	37.0	37.0	37.0
2	36.2315	37.0	37.0	37.0	37.0	37.0
3	36.3395	37.0	37.0	37.0	37.0	37.0
4	36.352	37.0	37.0	37.0	37.0	37.0
5	36.4815	37.0	37.0	37.0	37.0	37.0
6	36.411	37.0	37.0	37.0	37.0	37.0
7	36.4265	37.0	37.0	37.0	37.0	37.0
8	36.356	37.0	37.0	37.0	37.0	37.0
9	36.433	37.0	37.0	37.0	37.0	37.0
10-11	36.438	37.0	37.0	37.0	37.0	37.0
12-13	36.45125	37.0	37.0	37.0	37.0	37.0
14-15	36.42675	37.0	37.0	37.0	37.0	37.0
16-17	36.496750000000006	37.0	37.0	37.0	37.0	37.0
18-19	36.46625	37.0	37.0	37.0	37.0	37.0
20-21	36.32225	37.0	37.0	37.0	37.0	37.0
22-23	36.344	37.0	37.0	37.0	37.0	37.0
24-25	36.355000000000004	37.0	37.0	37.0	37.0	37.0
26-27	36.329	37.0	37.0	37.0	37.0	37.0
28-29	36.2685	37.0	37.0	37.0	37.0	37.0
30-31	36.35925	37.0	37.0	37.0	37.0	37.0
32-33	36.255250000000004	37.0	37.0	37.0	37.0	37.0
34-35	36.304249999999996	37.0	37.0	37.0	37.0	37.0
36-37	36.247749999999996	37.0	37.0	37.0	37.0	37.0
38-39	36.206500000000005	37.0	37.0	37.0	37.0	37.0
40-41	36.25775	37.0	37.0	37.0	37.0	37.0
42-43	36.19225	37.0	37.0	37.0	37.0	37.0
44-45	36.1515	37.0	37.0	37.0	37.0	37.0
46-47	36.122	37.0	37.0	37.0	37.0	37.0
48-49	36.09825	37.0	37.0	37.0	37.0	37.0
50-51	36.124875	37.0	37.0	37.0	37.0	37.0
52-53	36.054500000000004	37.0	37.0	37.0	37.0	37.0
54-55	36.24375	37.0	37.0	37.0	37.0	37.0
56-57	36.19	37.0	37.0	37.0	37.0	37.0
58-59	36.13475	37.0	37.0	37.0	37.0	37.0
60-61	36.06675	37.0	37.0	37.0	37.0	37.0
62-63	36.048125	37.0	37.0	37.0	37.0	37.0
64-65	36.09175	37.0	37.0	37.0	37.0	37.0
66-67	36.04375	37.0	37.0	37.0	37.0	37.0
68-69	36.01525	37.0	37.0	37.0	37.0	37.0
70-71	35.960750000000004	37.0	37.0	37.0	37.0	37.0
72-73	35.942375	37.0	37.0	37.0	37.0	37.0
74-75	35.97725	37.0	37.0	37.0	37.0	37.0
76-77	36.0485	37.0	37.0	37.0	37.0	37.0
78-79	35.9615	37.0	37.0	37.0	37.0	37.0
80-81	35.87125	37.0	37.0	37.0	37.0	37.0
82-83	35.87925	37.0	37.0	37.0	37.0	37.0
84-85	35.81275	37.0	37.0	37.0	37.0	37.0
86-87	35.833749999999995	37.0	37.0	37.0	37.0	37.0
88-89	35.783	37.0	37.0	37.0	37.0	37.0
90-91	35.82025	37.0	37.0	37.0	37.0	37.0
92-93	35.735125	37.0	37.0	37.0	37.0	37.0
94-95	35.631249999999994	37.0	37.0	37.0	37.0	37.0
96-97	35.732875	37.0	37.0	37.0	37.0	37.0
98-99	35.6725	37.0	37.0	37.0	37.0	37.0
100-101	35.436875	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	4.0
26	4.0
27	9.0
28	11.0
29	16.0
30	21.0
31	26.0
32	51.0
33	78.0
34	177.0
35	517.0
36	2730.0
37	355.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.023779724655824	20.52565707133917	15.068836045056319	25.381727158948685
2	20.75	26.625	34.599999999999994	18.025
3	18.975	27.250000000000004	33.650000000000006	20.125
4	24.125	35.65	22.475	17.75
5	26.075	31.825	25.074999999999996	17.025000000000002
6	21.725	34.65	25.174999999999997	18.45
7	19.025	21.45	39.5	20.025000000000002
8	21.099999999999998	22.6	30.599999999999998	25.7
9	21.7	24.3	29.875	24.125
10-11	23.974999999999998	32.2	23.45	20.375
12-13	23.75	25.0	27.750000000000004	23.5
14-15	21.987499999999997	26.487500000000004	30.5	21.025
16-17	22.575	26.674999999999997	31.162499999999998	19.5875
18-19	22.287499999999998	25.924999999999997	29.9875	21.8
20-21	21.4125	27.800000000000004	30.475	20.3125
22-23	21.912499999999998	28.6875	28.9	20.5
24-25	22.412499999999998	27.750000000000004	28.6875	21.15
26-27	22.575	26.7125	28.749999999999996	21.9625
28-29	21.975	27.375	28.925	21.725
30-31	22.375	26.974999999999998	30.2375	20.4125
32-33	21.875	25.874999999999996	31.4	20.849999999999998
34-35	22.5	27.500000000000004	28.487499999999997	21.512500000000003
36-37	21.337500000000002	27.900000000000002	30.0875	20.674999999999997
38-39	21.8125	26.575	28.999999999999996	22.6125
40-41	21.2	27.825	29.6625	21.3125
42-43	21.625	28.5625	29.1875	20.625
44-45	21.0	28.349999999999998	30.075000000000003	20.575
46-47	22.2625	27.787499999999998	27.3625	22.5875
48-49	21.575	27.237499999999997	31.624999999999996	19.5625
50-51	20.302537817227154	28.87860982622828	28.20352544068008	22.615326915864483
52-53	21.975	28.475	29.212500000000002	20.3375
54-55	22.9875	27.6625	28.0625	21.2875
56-57	20.2875	27.8125	28.8875	23.0125
58-59	20.5	27.3625	29.599999999999998	22.537499999999998
60-61	20.1375	26.924999999999997	30.075000000000003	22.8625
62-63	21.752719089886234	27.65345668208526	28.30353794224278	22.290286285785722
64-65	22.9375	26.987499999999997	29.5375	20.5375
66-67	20.2875	26.2125	30.349999999999998	23.150000000000002
68-69	21.4875	28.249999999999996	29.7875	20.474999999999998
70-71	21.0375	29.4875	28.9875	20.4875
72-73	22.22777847230904	27.390923865483185	28.54106763345418	21.840230028753595
74-75	21.8	27.1	28.9875	22.112499999999997
76-77	22.05	27.200000000000003	28.1625	22.5875
78-79	21.4875	26.924999999999997	30.7625	20.825
80-81	21.3625	26.625	30.062499999999996	21.95
82-83	21.912499999999998	27.325	29.45	21.3125
84-85	20.0375	27.0125	29.812499999999996	23.1375
86-87	21.2375	28.1625	28.462500000000002	22.1375
88-89	22.0625	28.012500000000003	29.312500000000004	20.6125
90-91	22.8	27.2625	27.474999999999998	22.4625
92-93	21.915239404925615	29.366170771346418	28.491061382672832	20.22752844105513
94-95	20.95	29.925	28.1625	20.962500000000002
96-97	22.90286285785723	27.84098012251531	27.51593949243655	21.7402175271909
98-99	21.75	28.025	28.025	22.2
100-101	22.290286285785722	27.17839729966246	28.56607075884486	21.965245655706962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	2.0
22	2.5
23	3.0
24	4.5
25	3.5
26	3.5
27	5.0
28	13.5
29	29.0
30	56.0
31	116.0
32	162.0
33	146.5
34	122.5
35	103.5
36	103.5
37	132.5
38	111.0
39	90.0
40	130.0
41	186.0
42	226.0
43	219.5
44	229.5
45	242.5
46	215.0
47	203.5
48	161.5
49	108.5
50	106.0
51	99.5
52	82.0
53	69.5
54	58.0
55	74.5
56	55.5
57	25.5
58	54.0
59	62.0
60	32.0
61	18.5
62	24.5
63	16.5
64	4.0
65	2.5
66	2.5
67	3.5
68	5.0
69	5.5
70	5.5
71	6.0
72	6.0
73	6.5
74	11.0
75	8.0
76	4.0
77	10.5
78	8.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0125
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0125
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0125
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0125
94-95	0.0
96-97	0.0125
98-99	0.0
100-101	0.0125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.45131518227964	36.0
2	16.889709275496077	18.3
3	7.014305491462852	11.4
4	2.814951545916013	6.1
5	2.445777572681126	6.625
6	1.2921089063221043	4.2
7	0.4153207198892478	1.575
8	0.6922011998154131	3.0
9	0.6460544531610521	3.15
>10	1.338255652976465	9.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	33	0.8250000000000001	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	24	0.6	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	21	0.525	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	16	0.4	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	16	0.4	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	15	0.375	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	15	0.375	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	14	0.35000000000000003	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	13	0.325	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	13	0.325	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	13	0.325	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	13	0.325	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	12	0.3	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	12	0.3	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	11	0.27499999999999997	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	11	0.27499999999999997	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	11	0.27499999999999997	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	11	0.27499999999999997	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	11	0.27499999999999997	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	11	0.27499999999999997	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	10	0.25	No Hit
GTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTA	10	0.25	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	10	0.25	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	10	0.25	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	10	0.25	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	10	0.25	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	10	0.25	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	10	0.25	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	10	0.25	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	9	0.22499999999999998	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	9	0.22499999999999998	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	9	0.22499999999999998	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	9	0.22499999999999998	No Hit
ATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTTGAGTT	9	0.22499999999999998	No Hit
CTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACC	9	0.22499999999999998	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	9	0.22499999999999998	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	9	0.22499999999999998	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	9	0.22499999999999998	No Hit
AAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAG	9	0.22499999999999998	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	9	0.22499999999999998	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	9	0.22499999999999998	No Hit
GCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAG	9	0.22499999999999998	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	9	0.22499999999999998	No Hit
AGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTC	8	0.2	No Hit
CTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCA	8	0.2	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	8	0.2	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	8	0.2	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	8	0.2	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	8	0.2	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	8	0.2	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	8	0.2	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	8	0.2	No Hit
GGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGA	8	0.2	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	8	0.2	No Hit
GAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTT	8	0.2	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	8	0.2	No Hit
ACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTT	8	0.2	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	8	0.2	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	7	0.17500000000000002	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	7	0.17500000000000002	No Hit
AGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCT	7	0.17500000000000002	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	7	0.17500000000000002	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	7	0.17500000000000002	No Hit
GAGCCATACTGCATATGTGTTGAGTTGGTCAAGGGCTTAATTCATAAATT	7	0.17500000000000002	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	7	0.17500000000000002	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	7	0.17500000000000002	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	7	0.17500000000000002	No Hit
CCTGGAAACCCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAA	6	0.15	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	6	0.15	No Hit
TTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTAC	6	0.15	No Hit
TCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATG	6	0.15	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	6	0.15	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	6	0.15	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	6	0.15	No Hit
CTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACA	6	0.15	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	6	0.15	No Hit
GGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAA	6	0.15	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	6	0.15	No Hit
TTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGAT	6	0.15	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	6	0.15	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	6	0.15	No Hit
ATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCT	6	0.15	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	6	0.15	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	6	0.15	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	6	0.15	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	6	0.15	No Hit
AAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAA	6	0.15	No Hit
ATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTCATGTACTT	6	0.15	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	6	0.15	No Hit
CCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATA	6	0.15	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	6	0.15	No Hit
CTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTC	6	0.15	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	6	0.15	No Hit
GGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGG	6	0.15	No Hit
AGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAA	6	0.15	No Hit
GTCAAAAGGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGG	5	0.125	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	5	0.125	No Hit
TTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGT	5	0.125	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	5	0.125	No Hit
TCCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAAT	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
GAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAA	5	0.125	No Hit
TGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGAC	5	0.125	No Hit
TAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTAC	5	0.125	No Hit
TAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTC	5	0.125	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	5	0.125	No Hit
GTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACA	5	0.125	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	5	0.125	No Hit
GTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAG	5	0.125	No Hit
ATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCAA	5	0.125	No Hit
AAGAAACTTAAAGAGAAAAGAAAACTGTGAGGAGAGACCAGAAGTAGAAT	5	0.125	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	5	0.125	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	5	0.125	No Hit
GACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAA	5	0.125	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	5	0.125	No Hit
GTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCT	5	0.125	No Hit
CCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCA	5	0.125	No Hit
CAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTC	5	0.125	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	5	0.125	No Hit
TGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
GAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAA	5	0.125	No Hit
TACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGA	5	0.125	No Hit
GGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGG	5	0.125	No Hit
AGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAG	5	0.125	No Hit
CTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCA	5	0.125	No Hit
AATAAGCCTTGTACAGAACAGATCCACCTGCAAAACTTTAGCTGAGGTCA	5	0.125	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	5	0.125	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	5	0.125	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	5	0.125	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	5	0.125	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	5	0.125	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	5	0.125	No Hit
AGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATAC	5	0.125	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	5	0.125	No Hit
AGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGG	5	0.125	No Hit
GTCGGTGGTGACTGGCGGCGGTGAGGCGGCCGGTGGCGGCGGCGGCGGCG	5	0.125	No Hit
TGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTT	5	0.125	No Hit
AATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCA	5	0.125	No Hit
GAGCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGG	5	0.125	No Hit
CCAGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAG	5	0.125	No Hit
GAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTG	5	0.125	No Hit
GTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCT	5	0.125	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	5	0.125	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	5	0.125	No Hit
GCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAGAAGTGTGAAC	5	0.125	No Hit
ATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTT	5	0.125	No Hit
CCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAAT	5	0.125	No Hit
CCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0625	0.0	0.0	0.0	0.0
72-73	0.125	0.0	0.0	0.0	0.0
74-75	0.15	0.0	0.0	0.0	0.0
76-77	0.175	0.0	0.0	0.0	0.0
78-79	0.2375	0.0	0.0	0.0	0.0
80-81	0.2625	0.0	0.0	0.0	0.0
82-83	0.2875	0.0	0.0	0.0	0.0
84-85	0.375	0.0	0.0	0.0	0.0
86-87	0.48750000000000004	0.0	0.0	0.0	0.0
88-89	0.6125	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
Read 3964788 spots for SRR24882418.sra
Written 3964788 spots for SRR24882418.sra
Read 3964784 spots for SRR24882418.sra
Written 3964784 spots for SRR24882418.sra
SRR ids: ['SRR24882418.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_z443mfud
SRR24882418.sra spots: 31718276
blocks: [[1, 3964784], [3964785, 7929568], [7929569, 11894352], [11894353, 15859136], [15859137, 19823920], [19823921, 23788704], [23788705, 27753488], [27753489, 31718276]]
SRR24882418 file size 7660080
SRR24882418 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882418 SRR24882418_1.fastq SRR24882418_2.fastq
Input file:	SRR24882418_1.fastq
Paired file:	SRR24882418_2.fastq
trimmed:	SRR24882418-trimmed-pair1.fastq, SRR24882418-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 03:00:25 2026 >> started

Fri Jul  3 03:01:09 2026 >> done (44.190s)
31718276 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
31718276 (100.00%) read pairs available; of these:
  624555 ( 1.97%) trimmed read pairs available after processing
31093721 (98.03%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 63	       1	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	      15	  0.00%
 67	     520	  0.00%
 68	    4247	  0.01%
 69	    5351	  0.02%
 70	    6082	  0.02%
 71	    6642	  0.02%
 72	    7365	  0.02%
 73	    7630	  0.02%
 74	    8094	  0.03%
 75	    9325	  0.03%
 76	    9857	  0.03%
 77	   10320	  0.03%
 78	   10987	  0.03%
 79	   11546	  0.04%
 80	   12443	  0.04%
 81	   13457	  0.04%
 82	   13846	  0.04%
 83	   15078	  0.05%
 84	   15985	  0.05%
 85	   17131	  0.05%
 86	   17229	  0.05%
 87	   19090	  0.06%
 88	   20721	  0.07%
 89	   20613	  0.06%
 90	   22974	  0.07%
 91	   24459	  0.08%
 92	   25223	  0.08%
 93	   27646	  0.09%
 94	   28285	  0.09%
 95	   29586	  0.09%
 96	   30460	  0.10%
 97	   33692	  0.11%
 98	   34457	  0.11%
 99	   38024	  0.12%
100	   66173	  0.21%
101	31093721	 98.03%
31718276 reads passed initial QC


criterion=sequence-density
sequence-density=0.43
sequence-density-rank=1
fanout-score=2.82
fanout-score-rank=28
prefix-density=0.49
prefix-fanout=2.5
sequence=GATTCTGCCCAA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=25
fanout-score=37.17
fanout-score-rank=1
prefix-density=0.22
prefix-fanout=5.1
sequence=TTCCACAGTGGGTGGGCATCGTCCCCATTTACACAGATCTTGCTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTCTTGATTACTTCCTGGCTCCTGCCTCCCAAACTGGTTGCAGGG


criterion=sequence-density
sequence-density=0.44
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=30
prefix-density=0.44
prefix-fanout=2.0
sequence=TTGGGCAGAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=86.29
fanout-score-rank=1
prefix-density=0.13
prefix-fanout=3.9
sequence=GGGGAAACTTGTGAATGTAACTTGCCTGTGCCGTGTGACGGTCACGTGGGCCTGGTCTTTTGTGTGTGAGGCCCTTGACCGTGTGGCCTCTGCCTGGCTGTTTGGGGTCCTGCACGGCTTTCCCACCACCTGTAGCTCTTGTTGACCTGCCTGTTCACCTCATGAGTGAAGCGTCTGCCTGGCAGTGGGCCATGAACTGAGGGGTCTCTGTGTAGAGTAGAAGCTTCCTGTGCCTCCGGTTGCCAGGAGACAGCCTGTGCAGTTAAATGG
SRR24882418 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 03:01:23
                             Started mapping on |	Jul 03 03:01:23
                                    Finished on |	Jul 03 03:02:50
       Mapping speed, Million of reads per hour |	1312.48

                          Number of input reads |	31718276
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	25269065
                        Uniquely mapped reads % |	79.67%
                          Average mapped length |	200.55
                       Number of splices: Total |	11516262
            Number of splices: Annotated (sjdb) |	11421569
                       Number of splices: GT/AG |	11406919
                       Number of splices: GC/AG |	84106
                       Number of splices: AT/AC |	8282
               Number of splices: Non-canonical |	16955
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.62
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.63
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6442198
             % of reads mapped to multiple loci |	20.31%
        Number of reads mapped to too many loci |	2129
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	7013	7013	7013
N_multimapping	6442198	6442198	6442198
N_noFeature	2564259	22673014	3451870
N_ambiguous	2024636	1023409	274214
UnstrandedReadsAssigned:20680170 PositiveStrandReadsAssigned:1572642 NegativeStrandReadsAssigned:21542981
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882418 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882418-trimmed-pair1.fastq
                             SRR24882418-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,718,276 reads, 25,482,707 reads pseudoaligned
[quant] estimated average fragment length: 196.123
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,297 rounds

 109283 SRR24882418.ke.tsv
  52636 SRR24882418.se.tsv
 161919 total
==> SRR24882418.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	13.6667	0	0
ENSMUST00000196221.1	9	6.66667	0	0
ENSMUST00000179664.1	11	8.66667	0	0
ENSMUST00000178537.1	12	9.66667	0	0
ENSMUST00000178862.1	14	11.6667	0	0
ENSMUST00000179520.1	11	8.66667	0	0
ENSMUST00000179883.1	16	13.6667	0	0
ENSMUST00000195858.1	10	7.66667	0	0
ENSMUST00000179932.1	12	9.66667	0	0

==> SRR24882418.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882418 completed mapping pipeline successfully
