Starting /dee2/code/volunteer_pipeline.sh SRR24882415
    current disk space = 1374970428
    free memory = 197947636 
SRR24882415 SRAfilesize
803de55ef04eef9f65471708ed617f81  SRR24882415.sra
SRR24882415.sra file validated
SRR24882415 is paired end
SRR24882415 is conventional basespace
SRR24882415 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882415_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5195	37.0	37.0	37.0	37.0	37.0
2	36.40525	37.0	37.0	37.0	37.0	37.0
3	36.5265	37.0	37.0	37.0	37.0	37.0
4	36.5535	37.0	37.0	37.0	37.0	37.0
5	36.5935	37.0	37.0	37.0	37.0	37.0
6	36.5295	37.0	37.0	37.0	37.0	37.0
7	36.584	37.0	37.0	37.0	37.0	37.0
8	36.557	37.0	37.0	37.0	37.0	37.0
9	36.577	37.0	37.0	37.0	37.0	37.0
10-11	36.52375	37.0	37.0	37.0	37.0	37.0
12-13	36.561	37.0	37.0	37.0	37.0	37.0
14-15	36.592	37.0	37.0	37.0	37.0	37.0
16-17	36.562250000000006	37.0	37.0	37.0	37.0	37.0
18-19	36.588499999999996	37.0	37.0	37.0	37.0	37.0
20-21	36.5435	37.0	37.0	37.0	37.0	37.0
22-23	36.534499999999994	37.0	37.0	37.0	37.0	37.0
24-25	36.5615	37.0	37.0	37.0	37.0	37.0
26-27	36.51349999999999	37.0	37.0	37.0	37.0	37.0
28-29	36.4905	37.0	37.0	37.0	37.0	37.0
30-31	36.53875	37.0	37.0	37.0	37.0	37.0
32-33	36.50475	37.0	37.0	37.0	37.0	37.0
34-35	36.50675	37.0	37.0	37.0	37.0	37.0
36-37	36.4615	37.0	37.0	37.0	37.0	37.0
38-39	36.4215	37.0	37.0	37.0	37.0	37.0
40-41	36.49625	37.0	37.0	37.0	37.0	37.0
42-43	36.399	37.0	37.0	37.0	37.0	37.0
44-45	36.48375	37.0	37.0	37.0	37.0	37.0
46-47	36.482	37.0	37.0	37.0	37.0	37.0
48-49	36.47325	37.0	37.0	37.0	37.0	37.0
50-51	36.468	37.0	37.0	37.0	37.0	37.0
52-53	36.428250000000006	37.0	37.0	37.0	37.0	37.0
54-55	36.45825	37.0	37.0	37.0	37.0	37.0
56-57	36.353	37.0	37.0	37.0	37.0	37.0
58-59	36.46025	37.0	37.0	37.0	37.0	37.0
60-61	36.3655	37.0	37.0	37.0	37.0	37.0
62-63	36.49025	37.0	37.0	37.0	37.0	37.0
64-65	36.40625	37.0	37.0	37.0	37.0	37.0
66-67	36.384	37.0	37.0	37.0	37.0	37.0
68-69	36.39175	37.0	37.0	37.0	37.0	37.0
70-71	36.314750000000004	37.0	37.0	37.0	37.0	37.0
72-73	36.251000000000005	37.0	37.0	37.0	37.0	37.0
74-75	36.28875	37.0	37.0	37.0	37.0	37.0
76-77	36.295	37.0	37.0	37.0	37.0	37.0
78-79	36.191	37.0	37.0	37.0	37.0	37.0
80-81	36.23125	37.0	37.0	37.0	37.0	37.0
82-83	36.28575	37.0	37.0	37.0	37.0	37.0
84-85	36.30275	37.0	37.0	37.0	37.0	37.0
86-87	36.2315	37.0	37.0	37.0	37.0	37.0
88-89	36.205749999999995	37.0	37.0	37.0	37.0	37.0
90-91	36.210750000000004	37.0	37.0	37.0	37.0	37.0
92-93	36.172	37.0	37.0	37.0	37.0	37.0
94-95	36.13575	37.0	37.0	37.0	37.0	37.0
96-97	36.1405	37.0	37.0	37.0	37.0	37.0
98-99	36.1425	37.0	37.0	37.0	37.0	37.0
100-101	35.99325	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	2.0
26	3.0
27	5.0
28	7.0
29	15.0
30	13.0
31	29.0
32	41.0
33	48.0
34	79.0
35	257.0
36	2742.0
37	757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.425000000000004	11.25	12.875	38.45
2	16.25406351587897	16.9792448112028	37.23430857714429	29.532383095773945
3	17.95	22.05	31.125000000000004	28.875
4	25.900000000000002	28.249999999999996	23.575	22.275
5	24.5	29.775000000000002	24.75	20.974999999999998
6	18.95	35.75	28.199999999999996	17.1
7	15.825	26.025	42.5	15.65
8	21.75	23.1	31.275	23.875
9	21.325	24.925	31.275	22.475
10-11	19.8	33.525	26.0375	20.6375
12-13	22.225	25.2125	27.800000000000004	24.762500000000003
14-15	19.75	29.4375	28.4125	22.400000000000002
16-17	20.1625	30.025000000000002	25.8125	24.0
18-19	20.7625	31.912499999999998	25.137500000000003	22.1875
20-21	21.05	29.012500000000003	28.449999999999996	21.4875
22-23	23.474999999999998	28.012500000000003	27.6875	20.825
24-25	23.8375	30.625000000000004	23.6875	21.85
26-27	22.525000000000002	29.625	26.4625	21.3875
28-29	19.537499999999998	29.675	28.325	22.4625
30-31	22.375	27.175	27.9125	22.537499999999998
32-33	19.6375	27.224999999999998	29.912499999999998	23.225
34-35	17.95	30.049999999999997	27.737499999999997	24.2625
36-37	18.6125	28.799999999999997	30.75	21.837500000000002
38-39	19.3125	29.45	30.9625	20.275000000000002
40-41	19.7375	30.662499999999998	29.862499999999997	19.7375
42-43	20.325	31.324999999999996	27.8125	20.5375
44-45	18.5625	32.1	25.5375	23.799999999999997
46-47	21.462500000000002	30.862499999999997	24.725	22.95
48-49	19.3125	31.775	26.25	22.662499999999998
50-51	21.6625	27.212500000000002	28.3625	22.7625
52-53	20.962500000000002	27.800000000000004	27.675	23.5625
54-55	20.075000000000003	27.075	29.1625	23.6875
56-57	18.5	28.375	30.5	22.625
58-59	17.95	27.187499999999996	32.425	22.4375
60-61	19.2625	28.749999999999996	28.812500000000004	23.175
62-63	20.25	30.3	29.2	20.25
64-65	21.349999999999998	27.1	28.249999999999996	23.3
66-67	20.3	26.4625	31.112499999999997	22.125
68-69	19.4875	27.5125	28.9875	24.0125
70-71	21.075	27.9125	27.875	23.1375
72-73	19.05	30.025000000000002	28.6375	22.287499999999998
74-75	20.549999999999997	28.7375	28.712500000000002	22.0
76-77	21.2875	28.975	27.825	21.912499999999998
78-79	19.55	29.349999999999998	28.499999999999996	22.6
80-81	22.1375	27.1625	28.65	22.05
82-83	21.05	30.612499999999997	28.299999999999997	20.0375
84-85	18.0625	30.95	28.475	22.5125
86-87	20.9375	29.037499999999998	27.0	23.025000000000002
88-89	23.5	27.762500000000003	29.575000000000003	19.162499999999998
90-91	20.4125	29.625	28.65	21.3125
92-93	22.6125	27.800000000000004	28.8375	20.75
94-95	20.0625	30.675	27.3375	21.925
96-97	20.7625	29.612500000000004	25.6125	24.0125
98-99	18.7625	30.7875	27.975	22.475
100-101	21.5	29.575000000000003	28.3375	20.5875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	0.0
21	0.0
22	0.0
23	1.0
24	2.5
25	1.5
26	1.5
27	3.5
28	3.0
29	14.5
30	37.5
31	72.0
32	139.5
33	168.5
34	136.0
35	129.0
36	137.0
37	123.0
38	78.0
39	72.0
40	119.0
41	203.0
42	295.0
43	267.0
44	181.5
45	161.5
46	255.5
47	333.5
48	255.0
49	158.0
50	138.0
51	119.0
52	80.5
53	62.5
54	52.5
55	36.5
56	17.0
57	5.0
58	5.0
59	8.5
60	8.0
61	5.5
62	10.5
63	11.0
64	4.0
65	2.0
66	6.0
67	10.0
68	5.5
69	4.0
70	5.5
71	4.0
72	5.5
73	7.5
74	9.5
75	7.0
76	7.0
77	7.5
78	4.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.85680190930788	27.175
2	14.916467780429594	12.5
3	5.310262529832936	6.675000000000001
4	3.5202863961813846	5.8999999999999995
5	2.386634844868735	5.0
6	1.9689737470167064	4.95
7	1.133651551312649	3.325
8	1.0143198090692125	3.4000000000000004
9	0.6563245823389021	2.475
>10	4.176610978520286	27.200000000000003
>50	0.05966587112171838	1.4000000000000001
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	56	1.4000000000000001	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	47	1.175	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	35	0.8750000000000001	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	33	0.8250000000000001	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	31	0.775	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	28	0.7000000000000001	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	25	0.625	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	24	0.6	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	24	0.6	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	22	0.5499999999999999	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	22	0.5499999999999999	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	20	0.5	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	20	0.5	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	19	0.475	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	19	0.475	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	18	0.44999999999999996	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	18	0.44999999999999996	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	18	0.44999999999999996	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	18	0.44999999999999996	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	17	0.42500000000000004	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	17	0.42500000000000004	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	16	0.4	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	16	0.4	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	16	0.4	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	15	0.375	No Hit
GGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACT	15	0.375	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	15	0.375	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	15	0.375	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	15	0.375	No Hit
CTGGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAG	15	0.375	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	15	0.375	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	15	0.375	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	14	0.35000000000000003	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	14	0.35000000000000003	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	14	0.35000000000000003	No Hit
CCACGATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCG	14	0.35000000000000003	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	14	0.35000000000000003	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	13	0.325	No Hit
GTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACA	13	0.325	No Hit
GCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCA	13	0.325	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	13	0.325	No Hit
TGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCT	13	0.325	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	12	0.3	No Hit
CTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACG	12	0.3	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	12	0.3	No Hit
GTCTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTT	12	0.3	No Hit
CTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAA	12	0.3	No Hit
CTTGAGGGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCT	11	0.27499999999999997	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	11	0.27499999999999997	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	11	0.27499999999999997	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	11	0.27499999999999997	No Hit
TGTAGATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTAC	11	0.27499999999999997	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	11	0.27499999999999997	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	11	0.27499999999999997	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	11	0.27499999999999997	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	11	0.27499999999999997	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	11	0.27499999999999997	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	10	0.25	No Hit
CACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCT	10	0.25	No Hit
GTCAATTTCTACATCTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGAC	10	0.25	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	10	0.25	No Hit
AGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCAT	10	0.25	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	10	0.25	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	10	0.25	No Hit
CACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTT	10	0.25	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	10	0.25	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	10	0.25	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	10	0.25	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	10	0.25	No Hit
CGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGG	10	0.25	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	10	0.25	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	9	0.22499999999999998	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	9	0.22499999999999998	No Hit
CTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAAC	9	0.22499999999999998	No Hit
CTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAG	9	0.22499999999999998	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	9	0.22499999999999998	No Hit
GGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCA	9	0.22499999999999998	No Hit
GTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTG	9	0.22499999999999998	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	9	0.22499999999999998	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	9	0.22499999999999998	No Hit
CAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGAT	9	0.22499999999999998	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	9	0.22499999999999998	No Hit
GTTTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACAC	8	0.2	No Hit
CTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTC	8	0.2	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	8	0.2	No Hit
TCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTT	8	0.2	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	8	0.2	No Hit
GTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTA	8	0.2	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	8	0.2	No Hit
CTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGAACAT	8	0.2	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	8	0.2	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	8	0.2	No Hit
GTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGC	8	0.2	No Hit
CTCAAAGAGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTA	8	0.2	No Hit
GTCGTATTTCACTGTCACTTTTCCCTGCTGGTGGCGTCGTTGTTGCTGTT	8	0.2	No Hit
GCAGTATGGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTT	8	0.2	No Hit
CCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCA	8	0.2	No Hit
CACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTT	8	0.2	No Hit
CTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGC	8	0.2	No Hit
GCAGGGGCTTCCTGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAG	7	0.17500000000000002	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	7	0.17500000000000002	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	7	0.17500000000000002	No Hit
CTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCA	7	0.17500000000000002	No Hit
TTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAAC	7	0.17500000000000002	No Hit
CCTGTATTGGGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	7	0.17500000000000002	No Hit
GGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTAC	7	0.17500000000000002	No Hit
CATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAG	7	0.17500000000000002	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	7	0.17500000000000002	No Hit
GTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGAT	7	0.17500000000000002	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	7	0.17500000000000002	No Hit
CTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTG	7	0.17500000000000002	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	7	0.17500000000000002	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	7	0.17500000000000002	No Hit
GTGGGGTCCAAATCCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACC	7	0.17500000000000002	No Hit
GCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACTTAAAACGTCTT	7	0.17500000000000002	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	7	0.17500000000000002	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	7	0.17500000000000002	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	7	0.17500000000000002	No Hit
GTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACA	6	0.15	No Hit
TTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTG	6	0.15	No Hit
CTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTT	6	0.15	No Hit
AGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCT	6	0.15	No Hit
GCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATT	6	0.15	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	6	0.15	No Hit
GGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGC	6	0.15	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	6	0.15	No Hit
CCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGC	6	0.15	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	6	0.15	No Hit
CCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAAC	6	0.15	No Hit
GAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCCTACA	6	0.15	No Hit
CAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTACTCAAAGA	6	0.15	No Hit
GGACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGA	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAAC	6	0.15	No Hit
CGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGG	6	0.15	No Hit
CTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAA	6	0.15	No Hit
TTCCTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTG	6	0.15	No Hit
GGCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAA	6	0.15	No Hit
GCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGT	6	0.15	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	6	0.15	No Hit
CTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAGT	6	0.15	No Hit
CCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATC	6	0.15	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	6	0.15	No Hit
CTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCC	6	0.15	No Hit
TGACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTT	6	0.15	No Hit
GTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATA	6	0.15	No Hit
ATCCATTCTTCCAGCACCAACCGCTTACGAAGCTCCTTACGGTCGTATTT	6	0.15	No Hit
GTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCATTAAAATA	6	0.15	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	6	0.15	No Hit
GTAATTTCGAAGCTCTTTCCTCCTCGCTGTTGGCATCAAGAAGATCATCA	6	0.15	No Hit
GGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAAT	6	0.15	No Hit
CACACCAAAAAATGTTGTTGTAAAAGACAACTATGGTTATTTTTTTTTTT	6	0.15	No Hit
GCTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGA	5	0.125	No Hit
AGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATC	5	0.125	No Hit
TGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCT	5	0.125	No Hit
CCAGACTTCTAAACCAACAAAGGTCTTGAGGGGTCTTCCATCTCTACATT	5	0.125	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	5	0.125	No Hit
TTGGAAAGTCGATTGGATAGTGTTTTGCTGGGGCAAACACGTGAAGGAAT	5	0.125	No Hit
TGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAGCCCT	5	0.125	No Hit
CCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGA	5	0.125	No Hit
GCCATTAAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAG	5	0.125	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTT	5	0.125	No Hit
CTCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	5	0.125	No Hit
CCAAGTTGTAGTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTT	5	0.125	No Hit
GCTTCCTCATGCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCT	5	0.125	No Hit
CTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAA	5	0.125	No Hit
CTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGCTCACAT	5	0.125	No Hit
ATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGATCCA	5	0.125	No Hit
CTCTGATGAATTCCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGT	5	0.125	No Hit
GTAGCTTTGCCATTAAAATATTACAGTAATGAAAACCCTTTAAAAACAAC	5	0.125	No Hit
CTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGC	5	0.125	No Hit
CCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAGC	5	0.125	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	5	0.125	No Hit
GGACCATGAACCACTGAGAGAAACCAGAGATGAAACCAGACTTCTAAACC	5	0.125	No Hit
CTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCA	5	0.125	No Hit
ATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAGAAACCAGA	5	0.125	No Hit
TTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTT	5	0.125	No Hit
CTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTG	5	0.125	No Hit
TTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCC	5	0.125	No Hit
GAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATT	5	0.125	No Hit
GGCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGA	5	0.125	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	5	0.125	No Hit
TTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTTT	5	0.125	No Hit
GTCTACCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAA	5	0.125	No Hit
TTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTTGCCAT	5	0.125	No Hit
GCCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACA	5	0.125	No Hit
AAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACAC	5	0.125	No Hit
CCTGATACAAAGCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATA	5	0.125	No Hit
AGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGC	5	0.125	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	5	0.125	No Hit
TTTTTTTTTAAGAAAAGTATCTGTGGGGTCCAAATCCCAGGTTGCTTTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.0875	0.0	0.0	0.0	0.0
76-77	0.125	0.0	0.0	0.0	0.0
78-79	0.15	0.0	0.0	0.0	0.0
80-81	0.3	0.0	0.0	0.0	0.0
82-83	0.325	0.0	0.0	0.0	0.0
84-85	0.4	0.0	0.0	0.0	0.0
86-87	0.48750000000000004	0.0	0.0	0.0	0.0
88-89	0.675	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882415 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882415_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.413	37.0	37.0	37.0	37.0	37.0
2	36.358	37.0	37.0	37.0	37.0	37.0
3	36.4215	37.0	37.0	37.0	37.0	37.0
4	36.409	37.0	37.0	37.0	37.0	37.0
5	36.5175	37.0	37.0	37.0	37.0	37.0
6	36.434	37.0	37.0	37.0	37.0	37.0
7	36.435	37.0	37.0	37.0	37.0	37.0
8	36.4915	37.0	37.0	37.0	37.0	37.0
9	36.5285	37.0	37.0	37.0	37.0	37.0
10-11	36.49725	37.0	37.0	37.0	37.0	37.0
12-13	36.49075	37.0	37.0	37.0	37.0	37.0
14-15	36.458749999999995	37.0	37.0	37.0	37.0	37.0
16-17	36.43	37.0	37.0	37.0	37.0	37.0
18-19	36.44575	37.0	37.0	37.0	37.0	37.0
20-21	36.3715	37.0	37.0	37.0	37.0	37.0
22-23	36.408500000000004	37.0	37.0	37.0	37.0	37.0
24-25	36.338499999999996	37.0	37.0	37.0	37.0	37.0
26-27	36.38525	37.0	37.0	37.0	37.0	37.0
28-29	36.3455	37.0	37.0	37.0	37.0	37.0
30-31	36.417249999999996	37.0	37.0	37.0	37.0	37.0
32-33	36.366749999999996	37.0	37.0	37.0	37.0	37.0
34-35	36.327	37.0	37.0	37.0	37.0	37.0
36-37	36.33	37.0	37.0	37.0	37.0	37.0
38-39	36.4385	37.0	37.0	37.0	37.0	37.0
40-41	36.365750000000006	37.0	37.0	37.0	37.0	37.0
42-43	36.291	37.0	37.0	37.0	37.0	37.0
44-45	36.277	37.0	37.0	37.0	37.0	37.0
46-47	36.17775	37.0	37.0	37.0	37.0	37.0
48-49	36.25175	37.0	37.0	37.0	37.0	37.0
50-51	36.19475	37.0	37.0	37.0	37.0	37.0
52-53	36.202	37.0	37.0	37.0	37.0	37.0
54-55	36.239000000000004	37.0	37.0	37.0	37.0	37.0
56-57	36.15975	37.0	37.0	37.0	37.0	37.0
58-59	36.179500000000004	37.0	37.0	37.0	37.0	37.0
60-61	36.145250000000004	37.0	37.0	37.0	37.0	37.0
62-63	36.12925	37.0	37.0	37.0	37.0	37.0
64-65	36.189750000000004	37.0	37.0	37.0	37.0	37.0
66-67	36.10575	37.0	37.0	37.0	37.0	37.0
68-69	35.95975	37.0	37.0	37.0	37.0	37.0
70-71	35.91275	37.0	37.0	37.0	37.0	37.0
72-73	35.985749999999996	37.0	37.0	37.0	37.0	37.0
74-75	36.12325	37.0	37.0	37.0	37.0	37.0
76-77	35.98375	37.0	37.0	37.0	37.0	37.0
78-79	35.997749999999996	37.0	37.0	37.0	37.0	37.0
80-81	35.98825	37.0	37.0	37.0	37.0	37.0
82-83	35.9245	37.0	37.0	37.0	37.0	37.0
84-85	36.00325	37.0	37.0	37.0	37.0	37.0
86-87	35.8745	37.0	37.0	37.0	37.0	37.0
88-89	35.837	37.0	37.0	37.0	37.0	37.0
90-91	35.8345	37.0	37.0	37.0	37.0	37.0
92-93	35.78725	37.0	37.0	37.0	37.0	37.0
94-95	35.7325	37.0	37.0	37.0	37.0	37.0
96-97	35.783500000000004	37.0	37.0	37.0	37.0	37.0
98-99	35.77875	37.0	37.0	37.0	37.0	37.0
100-101	35.4675	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	0.0
24	0.0
25	4.0
26	5.0
27	3.0
28	12.0
29	10.0
30	23.0
31	27.0
32	47.0
33	66.0
34	158.0
35	480.0
36	2770.0
37	393.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.78978978978979	18.76876876876877	14.914914914914915	26.526526526526528
2	19.15	26.025	36.75	18.075
3	20.549999999999997	26.174999999999997	34.150000000000006	19.125
4	24.075	32.45	25.2	18.275
5	22.85	31.1	28.1	17.95
6	21.349999999999998	34.55	25.025	19.075
7	19.175	22.2	39.225	19.400000000000002
8	23.400000000000002	21.349999999999998	31.05	24.2
9	25.05	23.825	29.225	21.9
10-11	24.1125	31.837500000000002	24.125	19.925
12-13	25.337500000000002	25.25	27.800000000000004	21.6125
14-15	23.7375	25.825	30.587500000000002	19.85
16-17	22.7	27.737499999999997	31.85	17.712500000000002
18-19	22.9875	28.425	28.449999999999996	20.1375
20-21	22.237499999999997	29.762499999999996	29.15	18.85
22-23	22.275	28.5625	28.0875	21.075
24-25	21.875	29.8375	28.1875	20.1
26-27	23.825	27.462500000000002	28.050000000000004	20.6625
28-29	23.825	26.737499999999997	29.912499999999998	19.525000000000002
30-31	25.900000000000002	25.4625	30.075000000000003	18.5625
32-33	22.8375	27.250000000000004	30.2125	19.7
34-35	22.662499999999998	28.125	29.312500000000004	19.900000000000002
36-37	23.200000000000003	27.575	29.862499999999997	19.3625
38-39	22.4375	27.0625	28.8625	21.637500000000003
40-41	20.3875	30.562499999999996	28.299999999999997	20.75
42-43	21.625	29.65	29.462500000000002	19.2625
44-45	22.662499999999998	29.6875	29.062500000000004	18.587500000000002
46-47	23.799999999999997	28.475	26.337500000000002	21.3875
48-49	22.525000000000002	27.462500000000002	30.1375	19.875
50-51	21.9	27.400000000000002	28.849999999999998	21.85
52-53	22.95	28.8875	29.1875	18.975
54-55	25.674999999999997	28.575	27.0125	18.7375
56-57	20.575	29.1875	28.3375	21.9
58-59	21.725	31.2125	28.349999999999998	18.712500000000002
60-61	22.1	29.3375	28.6375	19.925
62-63	22.4875	29.9	27.700000000000003	19.9125
64-65	23.8375	27.900000000000002	29.775000000000002	18.4875
66-67	22.787499999999998	25.724999999999998	29.625	21.8625
68-69	23.3	28.849999999999998	28.287499999999998	19.5625
70-71	20.525	28.425	31.587500000000002	19.4625
72-73	22.6875	28.749999999999996	27.500000000000004	21.0625
74-75	23.7125	27.525	29.6875	19.075
76-77	22.825	29.125	27.737499999999997	20.3125
78-79	23.8875	27.8875	28.462500000000002	19.7625
80-81	22.8625	27.6375	30.075000000000003	19.425
82-83	20.4125	27.625	31.4875	20.474999999999998
84-85	20.525	27.1625	30.5	21.8125
86-87	22.4375	27.675	29.375	20.5125
88-89	22.650000000000002	27.425	29.775000000000002	20.150000000000002
90-91	23.1	29.562500000000004	26.6	20.7375
92-93	22.6125	29.875	28.1875	19.325
94-95	21.2625	30.4	27.950000000000003	20.3875
96-97	23.425	28.050000000000004	28.6375	19.8875
98-99	21.5	29.099999999999998	28.1125	21.2875
100-101	22.6	28.0625	29.45	19.8875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.5
21	1.5
22	0.5
23	2.0
24	3.0
25	2.5
26	4.0
27	3.5
28	3.0
29	9.0
30	61.5
31	156.0
32	199.0
33	167.0
34	149.5
35	134.0
36	124.0
37	127.5
38	113.5
39	117.5
40	135.0
41	163.5
42	194.0
43	222.5
44	239.5
45	232.5
46	214.0
47	196.5
48	170.0
49	124.0
50	103.5
51	97.0
52	70.5
53	49.0
54	49.0
55	57.0
56	36.0
57	13.5
58	17.0
59	21.5
60	12.5
61	6.5
62	13.0
63	11.5
64	6.0
65	4.0
66	3.0
67	5.0
68	4.5
69	7.5
70	6.5
71	9.0
72	15.5
73	12.0
74	16.5
75	21.5
76	22.0
77	22.5
78	11.5
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.05
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.620689655172406	24.65
2	16.825208085612367	14.149999999999999
3	8.204518430439952	10.35
4	4.161712247324614	7.000000000000001
5	3.032104637336504	6.375
6	2.140309155766944	5.4
7	1.7241379310344827	5.075
8	1.3079667063020213	4.3999999999999995
9	0.7728894173602854	2.9250000000000003
>10	3.2104637336504163	19.675
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	41	1.0250000000000001	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	38	0.95	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	28	0.7000000000000001	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	26	0.65	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	26	0.65	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	21	0.525	No Hit
GGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAAATG	21	0.525	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	20	0.5	No Hit
TCTACAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCA	19	0.475	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	17	0.42500000000000004	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	16	0.4	No Hit
GGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTA	16	0.4	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	16	0.4	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	15	0.375	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	15	0.375	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	15	0.375	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	15	0.375	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	14	0.35000000000000003	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	14	0.35000000000000003	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	14	0.35000000000000003	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	14	0.35000000000000003	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	14	0.35000000000000003	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	13	0.325	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	13	0.325	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	13	0.325	No Hit
CTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGAC	12	0.3	No Hit
GGAAGAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATG	12	0.3	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	12	0.3	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	12	0.3	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	12	0.3	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	11	0.27499999999999997	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	11	0.27499999999999997	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	11	0.27499999999999997	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	11	0.27499999999999997	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	11	0.27499999999999997	No Hit
GCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGAT	11	0.27499999999999997	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	11	0.27499999999999997	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	11	0.27499999999999997	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	11	0.27499999999999997	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	11	0.27499999999999997	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	11	0.27499999999999997	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	11	0.27499999999999997	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	11	0.27499999999999997	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	10	0.25	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	10	0.25	No Hit
GGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGA	10	0.25	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	10	0.25	No Hit
CAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATA	10	0.25	No Hit
GGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGGTA	10	0.25	No Hit
CTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTG	10	0.25	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	10	0.25	No Hit
TTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGA	10	0.25	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	10	0.25	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	10	0.25	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	9	0.22499999999999998	No Hit
CACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGT	9	0.22499999999999998	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	9	0.22499999999999998	No Hit
CTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAA	9	0.22499999999999998	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	9	0.22499999999999998	No Hit
GGCGGCCGGTGGCGGCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTC	9	0.22499999999999998	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	9	0.22499999999999998	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	9	0.22499999999999998	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	9	0.22499999999999998	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	9	0.22499999999999998	No Hit
GGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTT	9	0.22499999999999998	No Hit
CAGACACTAAGTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAA	9	0.22499999999999998	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	9	0.22499999999999998	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	8	0.2	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	8	0.2	No Hit
AATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGAAGAAGAA	8	0.2	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	8	0.2	No Hit
GACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCT	8	0.2	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	8	0.2	No Hit
GGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGG	8	0.2	No Hit
CAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCA	8	0.2	No Hit
CAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATT	8	0.2	No Hit
CTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTCTC	8	0.2	No Hit
CACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAA	8	0.2	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	8	0.2	No Hit
AAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACT	8	0.2	No Hit
CATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAA	8	0.2	No Hit
CTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCC	8	0.2	No Hit
CTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGC	8	0.2	No Hit
GTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGA	8	0.2	No Hit
GTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTAC	8	0.2	No Hit
GCAACAGCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTGAAATACG	8	0.2	No Hit
GGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAG	8	0.2	No Hit
TTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTC	8	0.2	No Hit
CCACTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAATG	8	0.2	No Hit
GAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGAG	7	0.17500000000000002	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	7	0.17500000000000002	No Hit
GTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACC	7	0.17500000000000002	No Hit
ATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACA	7	0.17500000000000002	No Hit
CCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTT	7	0.17500000000000002	No Hit
ACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAA	7	0.17500000000000002	No Hit
GGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTT	7	0.17500000000000002	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	7	0.17500000000000002	No Hit
CCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTAC	7	0.17500000000000002	No Hit
GGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACT	7	0.17500000000000002	No Hit
CCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCC	7	0.17500000000000002	No Hit
GCTACTACAACTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTAC	7	0.17500000000000002	No Hit
TTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACA	7	0.17500000000000002	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	7	0.17500000000000002	No Hit
GTTGTCTTTTACAACAACATTTTTTGGTGTGTATGTGTGTGTGTTGGGGG	7	0.17500000000000002	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	7	0.17500000000000002	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	7	0.17500000000000002	No Hit
ATTTTTTGGTGTGTATGTGTGTGTGTTGGGGGGTAGACATTTATTCAAAA	7	0.17500000000000002	No Hit
GAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGC	7	0.17500000000000002	No Hit
AACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGA	7	0.17500000000000002	No Hit
GTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCAGT	7	0.17500000000000002	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	7	0.17500000000000002	No Hit
CTCGGCGCCGGTGACTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAAC	7	0.17500000000000002	No Hit
ATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTCC	7	0.17500000000000002	No Hit
GAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAG	7	0.17500000000000002	No Hit
CCCTATTAGATGTGGTCACAACACACGTGCTTTCAAAAGGTCTCCAGACA	7	0.17500000000000002	No Hit
GTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCT	7	0.17500000000000002	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	7	0.17500000000000002	No Hit
GCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTAC	7	0.17500000000000002	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	6	0.15	No Hit
CCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCA	6	0.15	No Hit
TTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTAC	6	0.15	No Hit
GATGCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGT	6	0.15	No Hit
AGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCT	6	0.15	No Hit
GAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACC	6	0.15	No Hit
CTCTCGTAGACTGCTATAAACCAACAGAGGAATTCATCAGAGAGCTGCTC	6	0.15	No Hit
CAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAG	6	0.15	No Hit
CTTCTTTTGTTGTTTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGC	6	0.15	No Hit
CAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCTGCA	6	0.15	No Hit
ACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTC	6	0.15	No Hit
GCCTGATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATC	6	0.15	No Hit
GGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGA	6	0.15	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	6	0.15	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	6	0.15	No Hit
CAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCT	6	0.15	No Hit
CTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTG	6	0.15	No Hit
GCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAAT	6	0.15	No Hit
TCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGT	6	0.15	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	6	0.15	No Hit
GAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCAT	6	0.15	No Hit
CTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGT	6	0.15	No Hit
GAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAA	6	0.15	No Hit
CTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATG	6	0.15	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	6	0.15	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	6	0.15	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	6	0.15	No Hit
CTTTGTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTC	6	0.15	No Hit
GCGGCGGCGGCGGTGCGCGTGTCTTCTTCCAGAGTCCCCGCGGTGGAACC	6	0.15	No Hit
TGTGTGTGTTGGGGGGTAGACATTTATTCAAAAGTGTTTCTTCTTTTGTT	6	0.15	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	6	0.15	No Hit
GCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAA	6	0.15	No Hit
GCAACAACGACGCCACCAGCAGGGAAAAGTGACAGTGAAATACGACCGTA	6	0.15	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	6	0.15	No Hit
TTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTAT	6	0.15	No Hit
CAAATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAAT	6	0.15	No Hit
TTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAATAACCATAGTTGT	5	0.125	No Hit
TTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGT	5	0.125	No Hit
CTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATCCACTTTCT	5	0.125	No Hit
AAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTG	5	0.125	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	5	0.125	No Hit
GGAAACCAATGGGTTGAGCTTCCTTTTTAGAAGCAGATTCTGGCCTCTTC	5	0.125	No Hit
GCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAG	5	0.125	No Hit
ATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACT	5	0.125	No Hit
GTGTAATACACTGTATTAGCTTTGTCCATGAAAGCAGGTCAAAAGGAAAC	5	0.125	No Hit
CTTGATGCCAACAGCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCT	5	0.125	No Hit
CCACAAGGATGGACAAGGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTA	5	0.125	No Hit
CGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGT	5	0.125	No Hit
GTTGGTTTAGAAGTCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGG	5	0.125	No Hit
TGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACA	5	0.125	No Hit
TATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAA	5	0.125	No Hit
GTGTGTTGTTAGCAAAGAATCCTTAATCACATGTCCTTTCATGTGTTTGT	5	0.125	No Hit
GCTTTGTCCATGAAAGCAGGTCAAAAGGAAACCAATGGGTTGAGCTTCCT	5	0.125	No Hit
CTTTTTAGAAGCAGATTCTGGCCTCTTCTGTGCCAGTACCATCAGTCCAG	5	0.125	No Hit
GCAGAAGAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGAT	5	0.125	No Hit
AGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACA	5	0.125	No Hit
GTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGAT	5	0.125	No Hit
GTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTAT	5	0.125	No Hit
GCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCA	5	0.125	No Hit
AAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTA	5	0.125	No Hit
TGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACC	5	0.125	No Hit
CTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACACTAAGT	5	0.125	No Hit
GTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGG	5	0.125	No Hit
CACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCC	5	0.125	No Hit
AATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTT	5	0.125	No Hit
ACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAA	5	0.125	No Hit
CTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACTTTT	5	0.125	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	5	0.125	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	5	0.125	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	5	0.125	No Hit
GAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTACAGGG	5	0.125	No Hit
TTTTAAAGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAA	5	0.125	No Hit
AAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAAAA	5	0.125	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	5	0.125	No Hit
AATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGTCATGTACTTATT	5	0.125	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	5	0.125	No Hit
AGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATAC	5	0.125	No Hit
GGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAA	5	0.125	No Hit
CAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATG	5	0.125	No Hit
CCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTACCCTAACCCTAC	5	0.125	No Hit
GAAGAGCGTATGATTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAA	5	0.125	No Hit
ATGAGCCATACTGCATATGTGTTGAGTTGGTCAAGGGCTTAATTCATAAA	5	0.125	No Hit
ATTACAATGAGCCATACTGCATATGTGTTGAGTTGGTCAAGGGCTTAATT	5	0.125	No Hit
GGCCAGGTGTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAA	5	0.125	No Hit
GTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATG	5	0.125	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	5	0.125	No Hit
CACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.05	0.0	0.0	0.0	0.0
74-75	0.0875	0.0	0.0	0.0	0.0
76-77	0.1375	0.0	0.0	0.0	0.0
78-79	0.175	0.0	0.0	0.0	0.0
80-81	0.325	0.0	0.0	0.0	0.0
82-83	0.35	0.0	0.0	0.0	0.0
84-85	0.425	0.0	0.0	0.0	0.0
86-87	0.5	0.0	0.0	0.0	0.0
88-89	0.675	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670494 spots for SRR24882415.sra
Written 4670494 spots for SRR24882415.sra
Read 4670495 spots for SRR24882415.sra
Written 4670495 spots for SRR24882415.sra
SRR ids: ['SRR24882415.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bz02x5tn
SRR24882415.sra spots: 37363953
blocks: [[1, 4670494], [4670495, 9340988], [9340989, 14011482], [14011483, 18681976], [18681977, 23352470], [23352471, 28022964], [28022965, 32693458], [32693459, 37363953]]
SRR24882415 file size 9027388
SRR24882415 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882415 SRR24882415_1.fastq SRR24882415_2.fastq
Input file:	SRR24882415_1.fastq
Paired file:	SRR24882415_2.fastq
trimmed:	SRR24882415-trimmed-pair1.fastq, SRR24882415-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 04:12:44 2026 >> started

Fri Jul  3 04:13:20 2026 >> done (36.077s)
37363953 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
37363952 (100.00%) read pairs available; of these:
  896321 ( 2.40%) trimmed read pairs available after processing
36467631 (97.60%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 63	       1	  0.00%
 64	       0	  0.00%
 65	       2	  0.00%
 66	      29	  0.00%
 67	     933	  0.00%
 68	    6548	  0.02%
 69	    7643	  0.02%
 70	    8909	  0.02%
 71	    9997	  0.03%
 72	   11229	  0.03%
 73	   11800	  0.03%
 74	   12469	  0.03%
 75	   14015	  0.04%
 76	   14806	  0.04%
 77	   16253	  0.04%
 78	   16999	  0.05%
 79	   17430	  0.05%
 80	   18939	  0.05%
 81	   20191	  0.05%
 82	   21317	  0.06%
 83	   22601	  0.06%
 84	   24210	  0.06%
 85	   25301	  0.07%
 86	   26261	  0.07%
 87	   28246	  0.08%
 88	   30230	  0.08%
 89	   30446	  0.08%
 90	   33089	  0.09%
 91	   35730	  0.10%
 92	   36516	  0.10%
 93	   39098	  0.10%
 94	   40394	  0.11%
 95	   42484	  0.11%
 96	   43591	  0.12%
 97	   48114	  0.13%
 98	   49142	  0.13%
 99	   53873	  0.14%
100	   77485	  0.21%
101	36467631	 97.60%
37363952 reads passed initial QC


criterion=sequence-density
sequence-density=0.59
sequence-density-rank=1
fanout-score=1.82
fanout-score-rank=30
prefix-density=1.07
prefix-fanout=1.0
sequence=GCAAGGAATTTGTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAGGGCACCGGGCAGGTCATCGAGGTGGCCTGCAGCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAACATCCTTTCCAGGGCTTCAGCTCCATATTCAGCACCATGGCCACCAATCTTCCCCCAGGCAGCCTTGATGTTGCTTTTGTCTTCCCCAGAGAGCACCATGGTTTCTTCCTGAGTCTGTC


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=26
fanout-score=21.20
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=7.2
sequence=TTTTTTTTTAACCAAAATAAATAAATCAGGTGTGTCCCAGTGGAGGTCAAAAGAGGGAGGAAGGACAGCTGGCTCCTTGGGACAACTTGCTCTTATCCACTGATCTGCTTTTGACCTGCCTTGGCACAGCAAAAAAAAGAATTCGTCAACAGCTTCTGTGTGTTCAAGGGAACAACAGAGAAAATGAAGTGACTGGGGAGGGGTCAGGAGAGCAGAGGCAGCAGGACCAAGAGGCAGCAGTGCCTGGCGTTGGGAGCCAATGGAGCTTCTGGGCCGTACTCAGAGCCAAGCTCTCCAGGCGTGGTAGCTGGGGTAAGTTTCCCACATACAGCCCAAACCCAAGAAGGGTAGCAGGGCTAGACTAACATCACTGCTAAAGTCCTCTAAATACCCCATGACAATCTCTATTCACACAACATCCTGATGACTCCCAAATCCTGGCAGCTATAGTTCCTCCATGAGCTCTCAGCTTGGCCCTAGCAAGATGAAACTACTCCTGGCCTTGAATCTG


criterion=sequence-density
sequence-density=0.30
sequence-density-rank=1
fanout-score=2.32
fanout-score-rank=32
prefix-density=0.32
prefix-fanout=2.2
sequence=AAATTCCTTGCCTCTGTGAGCACCGTGCTGACCTCCAAGTACCGTTAAGCTGCCTTCTGCGGGGCTTGCCTTCTGGCCATGCCCTTCTTCTCTCCCTTGCACCTGTACCTCTTGGTCTTTGAATAAAGCCTGAGTAGGAAG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=28
fanout-score=49.14
fanout-score-rank=1
prefix-density=0.27
prefix-fanout=5.9
sequence=GAAGAAGAGAATGCGCAGGCTGAAGCGCAAGAGAAGAAAGATGAGGCAGAGGTCCAAGTAAGCCAGCCCGTGCACCTACGACGCCTGCAGGAGCAGAAGTGAGGGATGCTGAGGGCCGGGACAAGCTATCGGACTGTGTGCTGCCATCGGTAATGAGTCTCAGTAGACCTGGAACGTCACCTCGCCGCGATCGCCTGGAGAAATGACCGCCTTTCTTACAACCAAAACAGTCCCTCTGCCCTGGACCCCCGGCACTCTGGACT
SRR24882415 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 04:13:33
                             Started mapping on |	Jul 03 04:13:34
                                    Finished on |	Jul 03 04:15:02
       Mapping speed, Million of reads per hour |	1528.53

                          Number of input reads |	37363952
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	30687825
                        Uniquely mapped reads % |	82.13%
                          Average mapped length |	200.38
                       Number of splices: Total |	15261117
            Number of splices: Annotated (sjdb) |	15139611
                       Number of splices: GT/AG |	15109210
                       Number of splices: GC/AG |	120882
                       Number of splices: AT/AC |	10947
               Number of splices: Non-canonical |	20078
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.03%
                        Deletion average length |	3.94
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.61
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6668285
             % of reads mapped to multiple loci |	17.85%
        Number of reads mapped to too many loci |	2788
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	7842	7842	7842
N_multimapping	6668285	6668285	6668285
N_noFeature	2954500	27154520	4108976
N_ambiguous	2739724	1452853	334688
UnstrandedReadsAssigned:24993601 PositiveStrandReadsAssigned:2080452 NegativeStrandReadsAssigned:26244161
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882415 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882415-trimmed-pair1.fastq
                             SRR24882415-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 37,363,952 reads, 29,825,540 reads pseudoaligned
[quant] estimated average fragment length: 202.301
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,108 rounds

 109283 SRR24882415.ke.tsv
  52636 SRR24882415.se.tsv
 161919 total
==> SRR24882415.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR24882415.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	4
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882415 completed mapping pipeline successfully
