Starting /dee2/code/volunteer_pipeline.sh SRR24882429
    current disk space = 1384126384
    free memory = 198297428 
SRR24882429 SRAfilesize
739f8c96bc5f4915478b7ba8ca76e4c2  SRR24882429.sra
SRR24882429.sra file validated
SRR24882429 is paired end
SRR24882429 is conventional basespace
SRR24882429 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.55725	37.0	37.0	37.0	37.0	37.0
2	36.509	37.0	37.0	37.0	37.0	37.0
3	36.4075	37.0	37.0	37.0	37.0	37.0
4	36.5865	37.0	37.0	37.0	37.0	37.0
5	36.5575	37.0	37.0	37.0	37.0	37.0
6	36.6325	37.0	37.0	37.0	37.0	37.0
7	36.59125	37.0	37.0	37.0	37.0	37.0
8	36.6055	37.0	37.0	37.0	37.0	37.0
9	36.593	37.0	37.0	37.0	37.0	37.0
10-11	36.623999999999995	37.0	37.0	37.0	37.0	37.0
12-13	36.636250000000004	37.0	37.0	37.0	37.0	37.0
14-15	36.53575	37.0	37.0	37.0	37.0	37.0
16-17	36.63425	37.0	37.0	37.0	37.0	37.0
18-19	36.579750000000004	37.0	37.0	37.0	37.0	37.0
20-21	36.672	37.0	37.0	37.0	37.0	37.0
22-23	36.6105	37.0	37.0	37.0	37.0	37.0
24-25	36.60425	37.0	37.0	37.0	37.0	37.0
26-27	36.54375	37.0	37.0	37.0	37.0	37.0
28-29	36.5745	37.0	37.0	37.0	37.0	37.0
30-31	36.56175	37.0	37.0	37.0	37.0	37.0
32-33	36.50325	37.0	37.0	37.0	37.0	37.0
34-35	36.500249999999994	37.0	37.0	37.0	37.0	37.0
36-37	36.54375	37.0	37.0	37.0	37.0	37.0
38-39	36.5275	37.0	37.0	37.0	37.0	37.0
40-41	36.52375	37.0	37.0	37.0	37.0	37.0
42-43	36.48950000000001	37.0	37.0	37.0	37.0	37.0
44-45	36.50175	37.0	37.0	37.0	37.0	37.0
46-47	36.5205	37.0	37.0	37.0	37.0	37.0
48-49	36.442625	37.0	37.0	37.0	37.0	37.0
50-51	36.4315	37.0	37.0	37.0	37.0	37.0
52-53	36.497625	37.0	37.0	37.0	37.0	37.0
54-55	36.421875	37.0	37.0	37.0	37.0	37.0
56-57	36.41425	37.0	37.0	37.0	37.0	37.0
58-59	36.48025	37.0	37.0	37.0	37.0	37.0
60-61	36.423875	37.0	37.0	37.0	37.0	37.0
62-63	36.447375	37.0	37.0	37.0	37.0	37.0
64-65	36.403125	37.0	37.0	37.0	37.0	37.0
66-67	36.362125	37.0	37.0	37.0	37.0	37.0
68-69	36.344625	37.0	37.0	37.0	37.0	37.0
70-71	36.351375000000004	37.0	37.0	37.0	37.0	37.0
72-73	36.273875000000004	37.0	37.0	37.0	37.0	37.0
74-75	36.242	37.0	37.0	37.0	37.0	37.0
76-77	36.373125	37.0	37.0	37.0	37.0	37.0
78-79	36.363125	37.0	37.0	37.0	37.0	37.0
80-81	36.370374999999996	37.0	37.0	37.0	37.0	37.0
82-83	36.37125	37.0	37.0	37.0	37.0	37.0
84-85	36.348	37.0	37.0	37.0	37.0	37.0
86-87	36.329750000000004	37.0	37.0	37.0	37.0	37.0
88-89	36.2355	37.0	37.0	37.0	37.0	37.0
90-91	36.329	37.0	37.0	37.0	37.0	37.0
92-93	36.262	37.0	37.0	37.0	37.0	37.0
94-95	36.24475	37.0	37.0	37.0	37.0	37.0
96-97	36.196250000000006	37.0	37.0	37.0	37.0	37.0
98-99	36.2115	37.0	37.0	37.0	37.0	37.0
100-101	36.0375	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	1.0
24	2.0
25	3.0
26	2.0
27	7.0
28	4.0
29	7.0
30	19.0
31	26.0
32	35.0
33	46.0
34	76.0
35	222.0
36	2659.0
37	890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.30932733183296	9.852463115778946	11.952988247061766	40.88522130532633
2	17.45	15.049999999999999	36.925000000000004	30.575000000000003
3	17.318295739348372	19.473684210526315	32.907268170426065	30.30075187969925
4	25.3	26.775	22.85	25.074999999999996
5	24.725	28.95	25.05	21.275
6	20.575	31.55	28.625	19.25
7	16.079019754938734	23.25581395348837	44.51112778194549	16.15403850962741
8	19.875	22.225	34.225	23.674999999999997
9	21.575	22.35	34.35	21.725
10-11	22.425	30.85	25.724999999999998	21.0
12-13	21.7	26.174999999999997	27.487499999999997	24.637500000000003
14-15	19.975	27.987499999999997	29.299999999999997	22.7375
16-17	19.9125	27.625	27.6125	24.85
18-19	21.775	29.062500000000004	26.424999999999997	22.7375
20-21	21.7	27.075	28.475	22.75
22-23	22.3375	27.0875	27.287499999999998	23.2875
24-25	23.325000000000003	27.9125	25.924999999999997	22.8375
26-27	22.112499999999997	28.712500000000002	27.9375	21.2375
28-29	19.662499999999998	28.237499999999997	28.999999999999996	23.1
30-31	22.25	26.0625	28.95	22.7375
32-33	21.8875	25.0625	30.862499999999997	22.1875
34-35	19.6375	27.487499999999997	28.812500000000004	24.0625
36-37	23.200000000000003	26.887499999999996	30.099999999999998	19.8125
38-39	21.087500000000002	27.5125	30.925000000000004	20.474999999999998
40-41	21.637500000000003	29.1125	29.0875	20.1625
42-43	20.925	30.5125	28.0875	20.474999999999998
44-45	18.9625	30.112499999999997	28.1625	22.7625
46-47	22.5625	30.0375	24.7375	22.662499999999998
48-49	22.027753469183647	29.241155144393048	26.178272284035504	22.552819102387797
50-51	22.05	26.6125	28.449999999999996	22.8875
52-53	22.440305038129765	25.378172271533945	29.15364420552569	23.0278784848106
54-55	20.47755969496187	25.753219152394045	29.603700462557818	24.16552069008626
56-57	19.6875	27.400000000000002	29.65	23.2625
58-59	21.349999999999998	25.587500000000002	29.575000000000003	23.4875
60-61	19.927490936367047	29.066133266658333	27.078384798099762	23.92799099887486
62-63	22.740342542817853	27.640955119389925	26.92836604575572	22.690336292036505
64-65	22.165270658832352	26.515814476809602	27.440930116264532	23.87798474809351
66-67	24.24053006625828	25.415676959619955	28.84110513814227	21.502687835979497
68-69	22.477809726215778	26.390798849856235	26.878359794974372	24.253031628953618
70-71	23.72796599574947	27.378422302787847	27.390923865483185	21.502687835979497
72-73	21.77772221527691	28.453556694586823	28.30353794224278	21.465183147893487
74-75	21.75	27.1125	29.1125	22.025
76-77	24.16552069008626	26.340792599074884	28.291036379547442	21.202650331291412
78-79	24.40305038129766	25.353169146143266	28.716089511188898	21.527690961370173
80-81	23.465433179147393	26.803350418802353	27.565945743217902	22.165270658832352
82-83	23.225	27.775	28.075	20.925
84-85	21.837500000000002	28.812500000000004	25.6125	23.7375
86-87	22.8625	28.625	26.875	21.637500000000003
88-89	25.637500000000003	26.474999999999998	27.462500000000002	20.424999999999997
90-91	22.725	26.1125	28.4375	22.725
92-93	23.549999999999997	27.325	27.275	21.85
94-95	22.0875	29.15	26.05	22.7125
96-97	22.6125	27.6625	23.8875	25.837500000000002
98-99	20.8875	29.225	27.4125	22.475
100-101	23.7375	27.0625	27.35	21.85
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	1.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	0.5
19	0.0
20	0.0
21	0.0
22	0.5
23	3.0
24	3.0
25	12.0
26	17.5
27	6.5
28	5.0
29	11.0
30	29.0
31	58.5
32	103.0
33	124.0
34	105.0
35	136.5
36	136.0
37	104.0
38	83.0
39	52.5
40	79.0
41	143.5
42	226.5
43	201.5
44	144.0
45	171.5
46	238.0
47	280.5
48	212.0
49	158.5
50	159.5
51	134.5
52	106.5
53	87.0
54	85.0
55	86.0
56	63.5
57	44.0
58	63.0
59	101.0
60	77.5
61	32.5
62	28.0
63	21.0
64	7.5
65	3.0
66	2.0
67	3.5
68	3.5
69	7.0
70	7.5
71	4.0
72	4.0
73	2.5
74	1.0
75	3.5
76	4.5
77	3.5
78	3.5
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.25
4	0.0
5	0.0
6	0.0
7	0.025
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0125
50-51	0.0
52-53	0.0125
54-55	0.0125
56-57	0.0
58-59	0.0
60-61	0.0125
62-63	0.0125
64-65	0.0125
66-67	0.0125
68-69	0.0125
70-71	0.0125
72-73	0.0125
74-75	0.0
76-77	0.0125
78-79	0.0125
80-81	0.0125
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.925000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.15072608913371	33.525
2	16.925388082123185	16.900000000000002
3	5.107661492238358	7.6499999999999995
4	3.104656985478217	6.2
5	1.8027040560841263	4.5
6	1.3019529293940912	3.9
7	0.8512769153730596	2.9749999999999996
8	1.1517275913870806	4.6
9	0.35052578868302453	1.575
>10	2.2533800701051576	18.175
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGA	42	1.05	No Hit
GCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAAG	41	1.0250000000000001	No Hit
CCTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACAC	35	0.8750000000000001	No Hit
GCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTA	26	0.65	No Hit
CCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACT	23	0.575	No Hit
CCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACA	23	0.575	No Hit
CTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAG	21	0.525	No Hit
GCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATAT	21	0.525	No Hit
CTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTTGTCCATCC	20	0.5	No Hit
GGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGCA	19	0.475	No Hit
CCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTA	18	0.44999999999999996	No Hit
CTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGG	18	0.44999999999999996	No Hit
CCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTT	17	0.42500000000000004	No Hit
GTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTC	16	0.4	No Hit
GTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTCTTTC	16	0.4	No Hit
CTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAG	16	0.4	No Hit
CTTTATTTGAGTAAATAGCTTGTAAATAAATCATTGGTAAACATTACAAA	16	0.4	No Hit
GTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAA	16	0.4	No Hit
GTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAGG	15	0.375	No Hit
GCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTG	15	0.375	No Hit
CTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGAC	15	0.375	No Hit
CCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTA	15	0.375	No Hit
GTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGCCTT	14	0.35000000000000003	No Hit
GTCCAGGGTTGCTTCCCAAATAGCCAGCTTCACTGACAGGCAGAGAGCGT	14	0.35000000000000003	No Hit
CTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAA	14	0.35000000000000003	No Hit
CTGTATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACC	13	0.325	No Hit
CTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTG	13	0.325	No Hit
GCCTTGTCCATCCTTGTGGATGTACCTGCTTTGATAGTTCTTGGTTGGGC	12	0.3	No Hit
ACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAA	12	0.3	No Hit
CTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTG	12	0.3	No Hit
GGCTCATTGTAATGACAAACACACTGAATTCTTTCACTTTGTTGCAGAAA	12	0.3	No Hit
CTTGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAA	11	0.27499999999999997	No Hit
CTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCTC	11	0.27499999999999997	No Hit
CACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGAT	11	0.27499999999999997	No Hit
CGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAGC	11	0.27499999999999997	No Hit
CTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACAC	11	0.27499999999999997	No Hit
ACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGATACAAAG	11	0.27499999999999997	No Hit
GCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGA	11	0.27499999999999997	No Hit
CCTCTGTTGGTTTATAGCAGTCTACGAGAGCTTCCTGTAATTTCGAAGCT	10	0.25	No Hit
GACCTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAG	10	0.25	No Hit
CTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGC	10	0.25	No Hit
TGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAAATCAAG	10	0.25	No Hit
CTCCTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAG	10	0.25	No Hit
AAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGTCC	10	0.25	No Hit
GCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGC	10	0.25	No Hit
TTTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCA	9	0.22499999999999998	No Hit
CTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTATAG	9	0.22499999999999998	No Hit
GGGTCTTCCATCTCTACATTTCGCAACACTCAGTTCACACTTCTGGACTG	9	0.22499999999999998	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	9	0.22499999999999998	No Hit
GACCTGGCAAAGCATCTCAACCAATGAAATAATAAAATTTATGAATTAAG	9	0.22499999999999998	No Hit
GCTCACATCTCTAGTTTTAACTTAGTGTCTGTAGATATATACAAGCATGA	9	0.22499999999999998	No Hit
GGCATTTCTTCTTCCTCGCAGCCGTACAGCTGACCCAGCTGCTCCACGAT	9	0.22499999999999998	No Hit
GGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGGAAGG	8	0.2	No Hit
CACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGA	8	0.2	No Hit
GCAATAAGTACATGACCTGGCAAAGCATCTCAACCAATGAAATAATAAAA	8	0.2	No Hit
GTTCAAAGCACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGG	8	0.2	No Hit
GCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTC	8	0.2	No Hit
GTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACCTGAT	8	0.2	No Hit
CTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGAG	8	0.2	No Hit
CTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAGG	8	0.2	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCC	8	0.2	No Hit
GAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCTA	8	0.2	No Hit
GCTGGGTTTCGTGTCTCTCTAGAACTTGCATCACGTGCTTCATAGTTTTT	8	0.2	No Hit
CCTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCT	8	0.2	No Hit
GCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATG	8	0.2	No Hit
GATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGCTC	8	0.2	No Hit
CCCAGGTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATC	8	0.2	No Hit
CCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATG	8	0.2	No Hit
CACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGA	8	0.2	No Hit
CCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGACAACTAT	8	0.2	No Hit
TTAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAA	8	0.2	No Hit
CCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTGGC	8	0.2	No Hit
CTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTAT	8	0.2	No Hit
GACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAACACACT	8	0.2	No Hit
CCCCTCTGTGCAGTTTTGATTGTCCCTCTGTCTTTATGTAGTTGAGTTCT	8	0.2	No Hit
TCTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCA	7	0.17500000000000002	No Hit
GGTAAACATTACAAAATTAAATACATTTTCAAAAGCTTGCCAGTATACGG	7	0.17500000000000002	No Hit
GGCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTG	7	0.17500000000000002	No Hit
TGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	7	0.17500000000000002	No Hit
GCCTCTTATCCGGGAGAGCAGCTCTCTGATGAATTCCTCTGTTGGTTTAT	7	0.17500000000000002	No Hit
TTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTTTATCAA	7	0.17500000000000002	No Hit
GCCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGA	7	0.17500000000000002	No Hit
ATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTCTCA	7	0.17500000000000002	No Hit
GCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTTCTT	7	0.17500000000000002	No Hit
GTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTC	7	0.17500000000000002	No Hit
CTTTTGACCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAA	7	0.17500000000000002	No Hit
GCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAA	7	0.17500000000000002	No Hit
GTTTTAACTTAGTGTCTGTAGATATATACAAGCATGAGATAGCTTTGACA	7	0.17500000000000002	No Hit
CTGAATTCTTTCACTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACA	7	0.17500000000000002	No Hit
GACTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAA	7	0.17500000000000002	No Hit
CGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAG	7	0.17500000000000002	No Hit
CTACATTTCGCAACACTCAGTTCACACTTCTGGACTGATGGTACTGGCAC	7	0.17500000000000002	No Hit
CTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCAT	6	0.15	No Hit
GGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAG	6	0.15	No Hit
GTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATG	6	0.15	No Hit
GCTGGTTTTCAAAATTAAGAGTTCATACAACTTTAAAAAACCACAATGAG	6	0.15	No Hit
CTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACGCTCT	6	0.15	No Hit
GCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCA	6	0.15	No Hit
CCCTTGACCAACTCAACACATATGCAGTATGGCTCATTGTAATGACAAAC	6	0.15	No Hit
GTTCTTTTAAAAAATAAAATATGTTCAAAGCACCCTTGATATAAATTGCC	6	0.15	No Hit
GAGAATTTTACCCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCT	6	0.15	No Hit
CTAACATGGAAATCAAGGTCACAATCAGGACCATGAACCACTGAGAGAAA	6	0.15	No Hit
CCTGCTTTGATAGTTCTTGGTTGGGCTACAGTCCAAGTTGTAGTAGCTTT	6	0.15	No Hit
ATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAGAGTTCATACAACTT	6	0.15	No Hit
GGCCAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATA	6	0.15	No Hit
ATTGTGAGATCCTAAATTACAGCCGAGTCCTGACAGAAAACACACCTGGC	6	0.15	No Hit
CTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTCTA	6	0.15	No Hit
GTTGCTTTCTTCATCGCCGAGAGAATTTTACCCTGTAGTGAAATCATACG	6	0.15	No Hit
GAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAACTTTTACCCTGCC	6	0.15	No Hit
CACCCTTGATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGG	6	0.15	No Hit
GTTGCTACCTGGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGT	6	0.15	No Hit
GTCTTACCAAACTGACCATAAGAAAACCATCTTCCCTGTATTGTGAGATC	6	0.15	No Hit
GGCTTTTACTCAACTTTTACCCTGCCTACATCCCATATGAATTTTAATTC	6	0.15	No Hit
GCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCATGCCTCTTATCCGGGAGA	6	0.15	No Hit
CTCAGTTCACACTTCTGGACTGATGGTACTGGCACAGAAGAGGCCAGAAT	6	0.15	No Hit
GGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTT	6	0.15	No Hit
AAAATATTACAGTAATGAAAACCCTTTAAAAACAACAAAAGAAGAAACAC	6	0.15	No Hit
GTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTC	6	0.15	No Hit
TTTTTACACTCCTTCTACCTCACCTTTGTTGTTTGTTTGTTTGTTTGTTT	5	0.125	No Hit
GACAGGGCCACCTGGAGACTTCTCACGGCAGGCAGGGTTTCCAGGGGGAG	5	0.125	No Hit
CTTTTGAAAGCACGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCT	5	0.125	No Hit
TGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATCT	5	0.125	No Hit
CTACCCCCCAACACACACACATACACACCAAAAAATGTTGTTGTAAAAGA	5	0.125	No Hit
GTCAATTTCTACATCTGGCATTTCTTCTTCCTCGCAGCCGTACAGCTGAC	5	0.125	No Hit
TGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTAACATGGAA	5	0.125	No Hit
CTGAAGAATGTCTGGAGACCTTTTGAAAGCACGTGTGTTGTGACCACATC	5	0.125	No Hit
CTTCTCACGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGG	5	0.125	No Hit
CAAAAAGTGTAGGTATTTCTTTATCAATCAGGCTGGTTTTCAAAATTAAG	5	0.125	No Hit
CCTGCTTTCATGGACAAAGCTAATACAGTGTATTACACTGAAAGCAGAGA	5	0.125	No Hit
AGCTGGTATGTAGCTGTAGGGTTAGGGTAGGTCATCAGACCTAGCTCCTA	5	0.125	No Hit
GACAGAAAACACACCTGGCCTTGTCCATCCTTGTGGATGTACCTGCTTTG	5	0.125	No Hit
CTTGTCATAAAGCTCTTCCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGG	5	0.125	No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGTAGGGCTTTTACTCAAC	5	0.125	No Hit
CCAGAATCTGCTTCTAAAAAGGAAGCTCAACCCATTGGTTTCCTTTTGAC	5	0.125	No Hit
CGTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAAC	5	0.125	No Hit
CTTTGTTGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAA	5	0.125	No Hit
GTCATAAAGCTCTTCCAGGTTTGCTCTGTCCCAGTAGAAGTCAGGGGTGA	5	0.125	No Hit
GTGTGTTGTGACCACATCTAATAGGGAAGCACAGGCTTATTTCATGAACC	5	0.125	No Hit
CTGATGGTACTGGCACAGAAGAGGCCAGAATCTGCTTCTAAAAAGGAAGC	5	0.125	No Hit
GATATAAATTGCCTGGTCCCTGTTTTCAGACAGGGCCACCTGGAGACTTC	5	0.125	No Hit
ACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGTTCAAAG	5	0.125	No Hit
CCTGTAGTGAAATCATACGCTCTTCTTCTGCGGAGGGCTCAGCTTCCTCA	5	0.125	No Hit
GATATATACAAGCATGAGATAGCTTTGACATTGAAGTCAAATTTACAACT	5	0.125	No Hit
CGGGCTTCCTTTTCTGATTGCAAAACTGTGTTCAAAAAGTGTAGGTATTT	5	0.125	No Hit
GCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGG	5	0.125	No Hit
GGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAGG	5	0.125	No Hit
CTGAAGGTGGACGACATTTGTCTGAAGAATGTCTGGAGACCTTTTGAAAG	5	0.125	No Hit
AAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAACAC	5	0.125	No Hit
TAAAAACAACAAAAGAAGAAACACTTTTGAATAAATGTCTACCCCCCAAC	5	0.125	No Hit
ATAAGAAAACCATCTTCCCTGTATTGTGAGATCCTAAATTACAGCCGAGT	5	0.125	No Hit
CGGCAGGCAGGGTTTCCAGGGGGAGGCAGCAGGGGCTTCCTGGAAGGAAG	5	0.125	No Hit
CTCAAACTGGGCAGTCTGCCTCTGCTTTTCCAGTGCAGAGATGAGTGCGT	5	0.125	No Hit
TGCAGAAAGTGGATGCTGAAGGTGGACGACATTTGTCTGAAGAATGTCTG	5	0.125	No Hit
CAGTATACGGAAAATTCACAAACATAGTTCTTTTAAAAAATAAAATATGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.1	0.0	0.0	0.0	0.0
72-73	0.1875	0.0	0.0	0.0	0.0
74-75	0.4	0.0	0.0	0.0	0.0
76-77	0.6125	0.0	0.0	0.0	0.0
78-79	0.7875	0.0	0.0	0.0	0.0
80-81	1.0	0.0	0.0	0.0	0.0
82-83	1.2625	0.0	0.0	0.0	0.0
84-85	1.5125	0.0	0.0	0.0	0.0
86-87	1.7125	0.0	0.0	0.0	0.0
88-89	1.825	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR24882429 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24882429_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.397	37.0	37.0	37.0	37.0	37.0
2	36.101	37.0	37.0	37.0	37.0	37.0
3	36.28	37.0	37.0	37.0	37.0	37.0
4	36.3425	37.0	37.0	37.0	37.0	37.0
5	36.371	37.0	37.0	37.0	37.0	37.0
6	36.347	37.0	37.0	37.0	37.0	37.0
7	36.3135	37.0	37.0	37.0	37.0	37.0
8	36.359	37.0	37.0	37.0	37.0	37.0
9	36.3955	37.0	37.0	37.0	37.0	37.0
10-11	36.354749999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.422250000000005	37.0	37.0	37.0	37.0	37.0
14-15	36.366749999999996	37.0	37.0	37.0	37.0	37.0
16-17	36.411	37.0	37.0	37.0	37.0	37.0
18-19	36.4395	37.0	37.0	37.0	37.0	37.0
20-21	36.375249999999994	37.0	37.0	37.0	37.0	37.0
22-23	36.24275	37.0	37.0	37.0	37.0	37.0
24-25	36.260999999999996	37.0	37.0	37.0	37.0	37.0
26-27	36.27575	37.0	37.0	37.0	37.0	37.0
28-29	36.36275	37.0	37.0	37.0	37.0	37.0
30-31	36.274	37.0	37.0	37.0	37.0	37.0
32-33	36.22425	37.0	37.0	37.0	37.0	37.0
34-35	36.19675	37.0	37.0	37.0	37.0	37.0
36-37	36.319500000000005	37.0	37.0	37.0	37.0	37.0
38-39	36.2535	37.0	37.0	37.0	37.0	37.0
40-41	36.236000000000004	37.0	37.0	37.0	37.0	37.0
42-43	36.2005	37.0	37.0	37.0	37.0	37.0
44-45	36.203500000000005	37.0	37.0	37.0	37.0	37.0
46-47	36.18575	37.0	37.0	37.0	37.0	37.0
48-49	36.13475	37.0	37.0	37.0	37.0	37.0
50-51	36.06725	37.0	37.0	37.0	37.0	37.0
52-53	36.09075	37.0	37.0	37.0	37.0	37.0
54-55	36.143	37.0	37.0	37.0	37.0	37.0
56-57	36.1145	37.0	37.0	37.0	37.0	37.0
58-59	36.10525	37.0	37.0	37.0	37.0	37.0
60-61	36.0475	37.0	37.0	37.0	37.0	37.0
62-63	36.081	37.0	37.0	37.0	37.0	37.0
64-65	36.036249999999995	37.0	37.0	37.0	37.0	37.0
66-67	35.98925	37.0	37.0	37.0	37.0	37.0
68-69	35.9055	37.0	37.0	37.0	37.0	37.0
70-71	35.971999999999994	37.0	37.0	37.0	37.0	37.0
72-73	36.00425	37.0	37.0	37.0	37.0	37.0
74-75	35.94025	37.0	37.0	37.0	37.0	37.0
76-77	35.980000000000004	37.0	37.0	37.0	37.0	37.0
78-79	35.958	37.0	37.0	37.0	37.0	37.0
80-81	35.84075	37.0	37.0	37.0	37.0	37.0
82-83	35.92775	37.0	37.0	37.0	37.0	37.0
84-85	35.8725	37.0	37.0	37.0	37.0	37.0
86-87	35.88525	37.0	37.0	37.0	37.0	37.0
88-89	35.82625	37.0	37.0	37.0	37.0	37.0
90-91	35.68475	37.0	37.0	37.0	37.0	37.0
92-93	35.72775	37.0	37.0	37.0	37.0	37.0
94-95	35.73350000000001	37.0	37.0	37.0	37.0	37.0
96-97	35.77975	37.0	37.0	37.0	37.0	37.0
98-99	35.7255	37.0	37.0	37.0	37.0	37.0
100-101	35.47525	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	4.0
24	3.0
25	3.0
26	5.0
27	8.0
28	8.0
29	16.0
30	19.0
31	25.0
32	43.0
33	86.0
34	133.0
35	601.0
36	2720.0
37	325.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.62907268170426	19.348370927318296	14.110275689223057	24.912280701754387
2	20.125	28.349999999999998	34.050000000000004	17.474999999999998
3	21.5	28.749999999999996	30.45	19.3
4	26.525	34.325	20.275000000000002	18.875
5	24.474999999999998	33.775	24.4	17.349999999999998
6	21.75	36.375	23.150000000000002	18.725
7	20.825	22.7	37.875	18.6
8	23.825	22.400000000000002	28.449999999999996	25.324999999999996
9	22.075	25.775	27.075	25.074999999999996
10-11	24.5625	32.237500000000004	23.125	20.075000000000003
12-13	25.95	24.75	26.200000000000003	23.1
14-15	23.8625	27.0	29.075	20.0625
16-17	24.5625	28.9125	28.3875	18.1375
18-19	23.875	28.65	27.85	19.625
20-21	23.400000000000002	28.8625	27.6375	20.1
22-23	23.1125	28.487499999999997	26.687499999999996	21.712500000000002
24-25	24.712500000000002	28.3375	26.187500000000004	20.7625
26-27	22.85	27.85	27.0875	22.2125
28-29	24.4875	27.3375	27.8375	20.3375
30-31	23.375	26.187500000000004	28.4125	22.025
32-33	23.9	26.1	29.4125	20.5875
34-35	22.425	28.212500000000002	26.8	22.5625
36-37	22.6125	27.537499999999998	29.1875	20.6625
38-39	22.575	27.0875	27.224999999999998	23.1125
40-41	21.85	28.549999999999997	27.85	21.75
42-43	22.037499999999998	29.312500000000004	26.950000000000003	21.7
44-45	22.7375	27.9375	29.9625	19.3625
46-47	23.425	28.012500000000003	24.9	23.6625
48-49	23.375	27.450000000000003	28.6875	20.4875
50-51	21.9	28.175	26.974999999999998	22.95
52-53	23.2375	27.375	29.2	20.1875
54-55	24.525	27.525	25.687500000000004	22.2625
56-57	20.7625	28.6625	28.849999999999998	21.725
58-59	20.95	28.6875	26.087500000000002	24.275
60-61	20.0875	29.975	27.625	22.3125
62-63	23.05	29.512500000000003	25.4	22.037499999999998
64-65	22.675	28.050000000000004	27.975	21.3
66-67	22.25	26.637499999999996	28.037499999999998	23.075000000000003
68-69	22.0875	30.075000000000003	27.762500000000003	20.075000000000003
70-71	21.6875	28.725	28.4375	21.15
72-73	22.287499999999998	28.812500000000004	26.900000000000002	22.0
74-75	23.724999999999998	27.6125	26.787499999999998	21.875
76-77	22.8125	27.625	25.724999999999998	23.8375
78-79	23.3375	27.325	28.1125	21.224999999999998
80-81	21.7375	28.7	28.3625	21.2
82-83	22.275	26.937499999999996	28.537499999999998	22.25
84-85	21.087500000000002	27.462500000000002	28.6375	22.8125
86-87	22.0125	30.0	26.150000000000002	21.837500000000002
88-89	23.2375	27.8125	27.275	21.675
90-91	23.3625	27.237499999999997	26.487500000000004	22.912499999999998
92-93	23.3375	29.325000000000003	28.1375	19.2
94-95	23.4875	29.512500000000003	25.7625	21.2375
96-97	24.5	26.787499999999998	26.55	22.162499999999998
98-99	22.5125	28.775000000000002	26.487500000000004	22.225
100-101	24.925	27.325	27.0	20.75
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	0.0
21	0.0
22	0.5
23	1.0
24	1.0
25	0.5
26	1.0
27	1.0
28	3.0
29	12.5
30	39.0
31	91.5
32	134.5
33	126.5
34	116.5
35	112.0
36	99.5
37	104.0
38	93.0
39	85.5
40	108.0
41	152.0
42	205.0
43	197.5
44	203.5
45	233.0
46	228.5
47	238.0
48	208.0
49	160.5
50	127.5
51	108.5
52	98.0
53	74.5
54	76.0
55	111.0
56	88.0
57	44.5
58	60.0
59	70.0
60	46.0
61	25.5
62	20.0
63	11.5
64	4.5
65	0.5
66	1.5
67	2.0
68	3.0
69	5.5
70	5.5
71	5.0
72	4.5
73	4.5
74	5.5
75	6.5
76	10.0
77	10.0
78	6.5
79	2.5
80	2.0
81	2.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.14929025942241	33.275
2	16.39745472344591	16.75
3	7.244248653940284	11.1
4	3.4263338228095934	7.000000000000001
5	2.447381302006853	6.25
6	1.2726382770435634	3.9
7	1.0279001468428781	3.675
8	0.7831620166421929	3.2
9	0.4405286343612335	2.025
>10	1.811062163485071	12.825000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTC	43	1.075	No Hit
GTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACTT	26	0.65	No Hit
GCTGTACGGCTGCGAGGAAGAAGAAATGCCAGATGTAGAAATTGACATTG	20	0.5	No Hit
ATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATTA	20	0.5	No Hit
GTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAAGCA	17	0.42500000000000004	No Hit
GGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAA	16	0.4	No Hit
GCTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGC	15	0.375	No Hit
CTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGG	15	0.375	No Hit
GTTAAAACTAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGT	14	0.35000000000000003	No Hit
GCTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGG	14	0.35000000000000003	No Hit
TTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACTA	14	0.35000000000000003	No Hit
AAAGAATTCAGTGTGTTTGTCATTACAATGAGCCATACTGCATATGTGTT	14	0.35000000000000003	No Hit
CTCGGCTGTAATTTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATG	13	0.325	No Hit
TTTCACTACAGGGTAAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGAT	13	0.325	No Hit
GTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTG	13	0.325	No Hit
GAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATC	13	0.325	No Hit
CTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTT	13	0.325	No Hit
GTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGAAGAATG	12	0.3	No Hit
TCTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGAC	12	0.3	No Hit
GTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC	12	0.3	No Hit
ATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATAC	12	0.3	No Hit
GTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGTGCTGGA	12	0.3	No Hit
GGCATGAGGAAGCTGAGCCCTCCGCAGAAGAAGAGCGTATGATTTCACTA	12	0.3	No Hit
GAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGCCAGGTGTGTT	12	0.3	No Hit
CTGGAAGAATGGATCGTGGAGCAGCTGGGTCAGCTGTACGGCTGCGAGGA	11	0.27499999999999997	No Hit
TAGAGATGTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCT	11	0.27499999999999997	No Hit
GGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCGTGAGAAGTCT	11	0.27499999999999997	No Hit
AAAATTCTCTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGA	11	0.27499999999999997	No Hit
GAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCT	11	0.27499999999999997	No Hit
GATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCT	11	0.27499999999999997	No Hit
ATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTC	10	0.25	No Hit
GCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGT	10	0.25	No Hit
GGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAGGG	10	0.25	No Hit
CATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGGAAGCTGAGCCCTC	10	0.25	No Hit
AAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACC	10	0.25	No Hit
CACAGTTTTGCAATCAGAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTG	10	0.25	No Hit
CTGGTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACC	10	0.25	No Hit
GGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAA	9	0.22499999999999998	No Hit
GTGAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGAT	9	0.22499999999999998	No Hit
GGATGATCGTCATCCTCATCACCATTGAGGTAATGTTTGAGCTGGGGAGA	9	0.22499999999999998	No Hit
GTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTT	9	0.22499999999999998	No Hit
GAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAA	9	0.22499999999999998	No Hit
TTAGGATCTCACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTA	9	0.22499999999999998	No Hit
GAAATACCTACACTTTTTGAACACAGTTTTGCAATCAGAAAAGGAAGCCC	9	0.22499999999999998	No Hit
CTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCT	9	0.22499999999999998	No Hit
GAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGT	9	0.22499999999999998	No Hit
GAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAAC	8	0.2	No Hit
CTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAA	8	0.2	No Hit
GTACCATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGG	8	0.2	No Hit
GTAGAATTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAA	8	0.2	No Hit
GCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCC	8	0.2	No Hit
TTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTA	8	0.2	No Hit
GCGAGGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTAT	8	0.2	No Hit
GTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTT	8	0.2	No Hit
TTAAAATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTA	8	0.2	No Hit
TGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTT	8	0.2	No Hit
GTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAGCAGGG	8	0.2	No Hit
TTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGAGCTTCG	8	0.2	No Hit
CCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCTAC	8	0.2	No Hit
GTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTA	8	0.2	No Hit
AAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTC	8	0.2	No Hit
CATTTATTCAAAAGTGTTTCTTCTTTTGTTGTTTTTAAAGGGTTTTCATT	8	0.2	No Hit
GAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTT	7	0.17500000000000002	No Hit
ACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCAAGACCTTTGTTG	7	0.17500000000000002	No Hit
ATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACA	7	0.17500000000000002	No Hit
GAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTCTTAAAAAAAAAA	7	0.17500000000000002	No Hit
CATCAGTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGA	7	0.17500000000000002	No Hit
AGGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGA	7	0.17500000000000002	No Hit
CTTCCTTCCAGGAAGCCCCTGCTGCCTCCCCCTGGAAACCCTGCCTGCCG	7	0.17500000000000002	No Hit
GTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACA	7	0.17500000000000002	No Hit
GAAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAA	7	0.17500000000000002	No Hit
AAGAAATGCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAAC	7	0.17500000000000002	No Hit
GCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAAGGC	7	0.17500000000000002	No Hit
AATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAACACACGTGCTTTCA	7	0.17500000000000002	No Hit
GTTGAGTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTG	7	0.17500000000000002	No Hit
GCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCAGGAAG	7	0.17500000000000002	No Hit
CTTATTGCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGA	7	0.17500000000000002	No Hit
CACGAAACCCAGCCCTATGAAGTGGCTCTCGTTCACTGGGAGAACGAAGA	7	0.17500000000000002	No Hit
CCTGCCTGCCGTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACC	7	0.17500000000000002	No Hit
GTTGGTCAAGGGCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATG	7	0.17500000000000002	No Hit
GCTTTGCCAGGTCATGTACTTATTGCCTCATTGTGGTTTTTTAAAGTTGT	7	0.17500000000000002	No Hit
GTGAGAAGTCTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTA	7	0.17500000000000002	No Hit
GTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCC	7	0.17500000000000002	No Hit
CAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCG	6	0.15	No Hit
GAAGAACTCAACTACATAAAGACAGAGGGACAATCAAAACTGCACAGAGG	6	0.15	No Hit
GCTTCGTAAGCGGTTGGTGCTGGAAGAATGGATCGTGGAGCAGCTGGGTC	6	0.15	No Hit
GGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTC	6	0.15	No Hit
AGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTT	6	0.15	No Hit
GCCTCATTGTGGTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCA	6	0.15	No Hit
CAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGTCCACCTTCAGCATC	6	0.15	No Hit
CTCCAGGTGGCCCTGTCTGAAAACAGGGACCAGGCAATTTATATCAAGGG	6	0.15	No Hit
TAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAGA	6	0.15	No Hit
AGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAACCAACAGA	6	0.15	No Hit
CTACATACCAGCTCTTTGAGTAGAATTAAAATTCATATGGGATGTAGGCA	6	0.15	No Hit
ATGGAAGACCCCTCAAGACCTTTGTTGGTTTAGAAGTCTGGTTTCATCTC	6	0.15	No Hit
CATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCC	6	0.15	No Hit
CTACGGTGGCCGCCGCCGGGCAAGTGCAACAGCAACAACGACGCCACCAG	6	0.15	No Hit
GGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAAATTTGACT	6	0.15	No Hit
GCCAGATGTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGG	6	0.15	No Hit
GTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGGTCTGATGACCT	6	0.15	No Hit
AGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCATGTTAGGAGCTAGG	6	0.15	No Hit
GCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGG	6	0.15	No Hit
GAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTT	6	0.15	No Hit
CTTTTCTTAAAAAAAAAAAATAACCATAGTTGTCTTTTACAACAACATTT	6	0.15	No Hit
GTGGAAAGTTCTGCAAAAGAAGGTGATCCCGGAACAATATTCTTGTTCAG	6	0.15	No Hit
CTTGGACTGTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGG	6	0.15	No Hit
CTCGGCGATGAAGAAAGCAACCTGGGATTTGGACCCCACAGATACTTTTC	6	0.15	No Hit
AATAAACCTGAGCTCAGATTTCTTGATCACCCCTGACTTCTACTGGGACA	6	0.15	No Hit
AATTCATATGGGATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTT	6	0.15	No Hit
TTTGGTAAGACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATC	5	0.125	No Hit
GAGCTTTGTATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGT	5	0.125	No Hit
GATGTAGGCAGGGTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCT	5	0.125	No Hit
GGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGTTGGT	5	0.125	No Hit
CCGGAACAATATTCTTGTTCAGGGAAGGAGCTGCTGTGTTCTGGAATGTA	5	0.125	No Hit
CCGGCGGCAGCCCAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGAC	5	0.125	No Hit
GACGTTTTAAGTTGTAAATTTGACTTCAATGTCAAAGCTATCTCATGCTT	5	0.125	No Hit
CCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTT	5	0.125	No Hit
ATTTTAATGGCAAAGCTACTACAACTTGGACTGTAGCCCAACCAAGAACT	5	0.125	No Hit
CTCACAACCTTGCACAAGCCACGTCCCTAGCCAGGCTGTACCTCAGCCTC	5	0.125	No Hit
ATGTCGTCCACCTTCAGCATCCACTTTCTGCAACAAAGTGAAAGAATTCA	5	0.125	No Hit
ACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTC	5	0.125	No Hit
GCCTGATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAATC	5	0.125	No Hit
GTCACAACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAAT	5	0.125	No Hit
GCAGGGAAAAGTGACAGTGAAATACGACCGTAAGGAGCTTCGTAAGCGGT	5	0.125	No Hit
CACAATACAGGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTT	5	0.125	No Hit
GTGTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGA	5	0.125	No Hit
CTTGGATGACGCCATTCTAGAGAAATTTGCGTTCTCCAACGCTCTCTGCC	5	0.125	No Hit
CTTTCTGCAACAAAGTGAAAGAATTCAGTGTGTTTGTCATTACAATGAGC	5	0.125	No Hit
AACACACGTGCTTTCAAAAGGTCTCCAGACATTCTTCAGACAAATGTCGT	5	0.125	No Hit
CCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCTCA	5	0.125	No Hit
TCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTGATTTCCAT	5	0.125	No Hit
GTAGAAATTGACATTGATGATCTTCTTGATGCCAACAGCGAGGAGGAAAG	5	0.125	No Hit
GGGAAGATGGTTTTCTTATGGTCAGTTTGGTAAGACGTTTTAAGTTGTAA	5	0.125	No Hit
GCTATCTCATGCTTGTATATATCTACAGACACTAAGTTAAAACTAGAGAT	5	0.125	No Hit
GAACTCTTAATTTTGAAAACCAGCCTGATTGATAAAGAAATACCTACACT	5	0.125	No Hit
GTAAAAGTTGAGTAAAAGCCCTACCTTCCTTCCTTCCTTCCTTCCTTCCT	5	0.125	No Hit
GTTTCATCTCTGGTTTCTCTCAGTGGTTCATGGTCCTGATTGTGACCTTG	5	0.125	No Hit
GAAAAGGAAGCCCCAGGTAGCAACATGTTCTCTGCTTTCAGTGTAATACA	5	0.125	No Hit
GGAGGAAAGAGCTTCGAAATTACAGGAAGCTCTCGTAGACTGCTATAAAC	5	0.125	No Hit
GTGGCTCTCGTTCACTGGGAGAACGAAGAACTCAACTACATAAAGACAGA	5	0.125	No Hit
CATGTTCTCTGCTTTCAGTGTAATACACTGTATTAGCTTTGTCCATGAAA	5	0.125	No Hit
GCTTAATTCATAAATTTTATTATTTCATTGGTTGAGATGCTTTGCCAGGT	5	0.125	No Hit
GTGGAAGTGACAAGCCTGCCTAGAGATGCAGCAAATATTTTGGTGATGGG	5	0.125	No Hit
GTAGCCCAACCAAGAACTATCAAAGCAGGTACATCCACAAGGATGGACAA	5	0.125	No Hit
GTCAAACTGTCTCATAAAGAAGTTATGCAGAAAATGGGTGAGCTCTTTGC	5	0.125	No Hit
CGCCATTCTAGAGAAATTTGCGTTCTCCAACGCTCTCTGCCTGTCAGTGA	5	0.125	No Hit
CACACCTAGAACCTCAGGAAGCTGAAGCAGGCAGATCCTGATGTCCAGAG	5	0.125	No Hit
TTTGACTTCAATGTCAAAGCTATCTCATGCTTGTATATATCTACAGACAC	5	0.125	No Hit
CTGGGACAGAGCAAACCTGGAAGAGCTTTATGACAAGACCTGCCAGTTCC	5	0.125	No Hit
CAGCCTGATTGATAAAGAAATACCTACACTTTTTGAACACAGTTTTGCAA	5	0.125	No Hit
CAACAGAGGAATTCATCAGAGAGCTGCTCTCCCGGATAAGAGGCATGAGG	5	0.125	No Hit
GTCCAGAAGTGTGAACTGAGTGTTGCGAAATGTAGAGATGGAAGACCCCT	5	0.125	No Hit
GCTAGGTCTGATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTG	5	0.125	No Hit
GATGACCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATT	5	0.125	No Hit
GGGTTTTCATTACTGTAATATTTTAATGGCAAAGCTACTACAACTTGGAC	5	0.125	No Hit
GTTTTCTGTCAGGACTCGGCTGTAATTTAGGATCTCACAATACAGGGAAG	5	0.125	No Hit
ATCAGGTTCATGAAATAAGCCTGTGCTTCCCTATTAGATGTGGTCACAAC	5	0.125	No Hit
CCTACCCTAACCCTACAGCTACATACCAGCTCTTTGAGTAGAATTAAAAT	5	0.125	No Hit
GTTTTTTAAAGTTGTATGAACTCTTAATTTTGAAAACCAGCCTGATTGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0125	0.0	0.0	0.0	0.0
70-71	0.1	0.0	0.0	0.0	0.0
72-73	0.1875	0.0	0.0	0.0	0.0
74-75	0.4	0.0	0.0	0.0	0.0
76-77	0.575	0.0	0.0	0.0	0.0
78-79	0.7375	0.0	0.0	0.0	0.0
80-81	0.95	0.0	0.0	0.0	0.0
82-83	1.2125	0.0	0.0	0.0	0.0
84-85	1.4625	0.0	0.0	0.0	0.0
86-87	1.6625	0.0	0.0	0.0	0.0
88-89	1.775	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGAAG	15	6.142176E-4	95.0	8
CATCAGT	15	6.142176E-4	95.0	1
TCCAGAA	15	6.142176E-4	95.0	7
ATCAGTC	15	6.142176E-4	95.0	2
GTCCAGA	15	6.142176E-4	95.0	6
CAGTCCA	20	0.0019257745	71.25	4
TCAGTCC	20	0.0019257745	71.25	3
CAGAAGT	20	0.0019257745	71.25	9
AGTCCAG	20	0.0019257745	71.25	5
GATGGAA	15	0.009957196	47.5	42-43
TGTGAAC	15	0.009957196	47.5	14-15
GTAGAGA	15	0.009957196	47.5	36-37
TGTTGCG	15	0.009957196	47.5	26-27
CTGAGTG	15	0.009957196	47.5	20-21
GTGTTGC	15	0.009957196	47.5	24-25
AGAAGTG	15	0.009957196	47.5	10-11
GAGTGTT	15	0.009957196	47.5	22-23
GAAATGT	15	0.009957196	47.5	32-33
GAAGTGT	15	0.009957196	47.5	10-11
TGCGAAA	15	0.009957196	47.5	28-29
>>END_MODULE
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
Read 3885679 spots for SRR24882429.sra
Written 3885679 spots for SRR24882429.sra
Read 3885674 spots for SRR24882429.sra
Written 3885674 spots for SRR24882429.sra
SRR ids: ['SRR24882429.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6glvyp18
SRR24882429.sra spots: 31085397
blocks: [[1, 3885674], [3885675, 7771348], [7771349, 11657022], [11657023, 15542696], [15542697, 19428370], [19428371, 23314044], [23314045, 27199718], [27199719, 31085397]]
SRR24882429 file size 7506800
SRR24882429 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24882429 SRR24882429_1.fastq SRR24882429_2.fastq
Input file:	SRR24882429_1.fastq
Paired file:	SRR24882429_2.fastq
trimmed:	SRR24882429-trimmed-pair1.fastq, SRR24882429-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Jul  3 02:19:23 2026 >> started

Fri Jul  3 02:19:47 2026 >> done (23.876s)
31085397 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       2 ( 0.00%) empty read pairs filtered out after trimming by size control
31085395 (100.00%) read pairs available; of these:
 1460175 ( 4.70%) trimmed read pairs available after processing
29625220 (95.30%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 63	       1	  0.00%
 64	       2	  0.00%
 65	       6	  0.00%
 66	      38	  0.00%
 67	    2167	  0.01%
 68	   11305	  0.04%
 69	   14164	  0.05%
 70	   15991	  0.05%
 71	   17740	  0.06%
 72	   19806	  0.06%
 73	   21042	  0.07%
 74	   22023	  0.07%
 75	   23986	  0.08%
 76	   26219	  0.08%
 77	   27960	  0.09%
 78	   29197	  0.09%
 79	   30719	  0.10%
 80	   32330	  0.10%
 81	   34514	  0.11%
 82	   36460	  0.12%
 83	   38291	  0.12%
 84	   41274	  0.13%
 85	   42962	  0.14%
 86	   43890	  0.14%
 87	   47138	  0.15%
 88	   50182	  0.16%
 89	   51175	  0.16%
 90	   55221	  0.18%
 91	   59290	  0.19%
 92	   59841	  0.19%
 93	   62670	  0.20%
 94	   64876	  0.21%
 95	   67756	  0.22%
 96	   69891	  0.22%
 97	   75972	  0.24%
 98	   76286	  0.25%
 99	   83676	  0.27%
100	  104114	  0.33%
101	29625220	 95.30%
31085395 reads passed initial QC


criterion=sequence-density
sequence-density=0.50
sequence-density-rank=1
fanout-score=4.61
fanout-score-rank=23
prefix-density=0.73
prefix-fanout=3.1
sequence=GGTTCAGCTCCA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=53.62
fanout-score-rank=1
prefix-density=0.48
prefix-fanout=2.1
sequence=CAAAGATCTTCACATTTTGCTCATCTCTTTGGTCACTTTCCTGCACTTGGATCAATCACTCTTCGCTTATGATTTTGGTGTGAACCTCCCGGCTCTTCACCCGCAGCTTGTTGACCTGGGACTCCGCGATGTCAGCCCGCTCCTCGGCTTCCTCCAGCTCGTGCTGGATCTTGCGGAA


criterion=sequence-density
sequence-density=1.17
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=33
prefix-density=1.17
prefix-fanout=2.0
sequence=CATCTTCTCCATC


criterion=fanout-score
sequence-density=0.23
sequence-density-rank=15
fanout-score=27.12
fanout-score-rank=1
prefix-density=1.03
prefix-fanout=6.0
sequence=GAGGAGCTGGAGGAGG
SRR24882429 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jul 03 02:20:02
                             Started mapping on |	Jul 03 02:20:02
                                    Finished on |	Jul 03 02:36:58
       Mapping speed, Million of reads per hour |	110.15

                          Number of input reads |	31085395
                      Average input read length |	200
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27323277
                        Uniquely mapped reads % |	87.90%
                          Average mapped length |	199.82
                       Number of splices: Total |	19017013
            Number of splices: Annotated (sjdb) |	18868741
                       Number of splices: GT/AG |	18876193
                       Number of splices: GC/AG |	110629
                       Number of splices: AT/AC |	10987
               Number of splices: Non-canonical |	19204
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.03%
                        Deletion average length |	3.95
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.69
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3752542
             % of reads mapped to multiple loci |	12.07%
        Number of reads mapped to too many loci |	4603
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9576	9576	9576
N_multimapping	3752542	3752542	3752542
N_noFeature	2022715	23662228	3102387
N_ambiguous	2824303	1310265	233492
UnstrandedReadsAssigned:22476259 PositiveStrandReadsAssigned:2350784 NegativeStrandReadsAssigned:23987398
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR24882429 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR24882429-trimmed-pair1.fastq
                             SRR24882429-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,085,395 reads, 25,369,471 reads pseudoaligned
[quant] estimated average fragment length: 179.528
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,224 rounds

 109283 SRR24882429.ke.tsv
  52636 SRR24882429.se.tsv
 161919 total
==> SRR24882429.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	12	0	0
ENSMUST00000196221.1	9	5	0	0
ENSMUST00000179664.1	11	7	0	0
ENSMUST00000178537.1	12	8	0	0
ENSMUST00000178862.1	14	10	0	0
ENSMUST00000179520.1	11	7	0	0
ENSMUST00000179883.1	16	12	0	0
ENSMUST00000195858.1	10	6	0	0
ENSMUST00000179932.1	12	8	0	0

==> SRR24882429.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24882429 completed mapping pipeline successfully
