Starting /dee2/code/volunteer_pipeline.sh SRR24920653
    current disk space = 442499803136
    free memory = 321397336 
SRR24920653 SRAfilesize
ae9f0714ca63eb7ee952b311ceb00bcb  SRR24920653.sra
SRR24920653.sra file validated
SRR24920653 is single end
SRR24920653 is conventional basespace
SRR24920653 read1 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24920653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.07	37.0	37.0	37.0	37.0	37.0
2	36.7115	37.0	37.0	37.0	37.0	37.0
3	35.9955	37.0	37.0	37.0	37.0	37.0
4	36.8095	37.0	37.0	37.0	37.0	37.0
5	36.6775	37.0	37.0	37.0	37.0	37.0
6	36.719	37.0	37.0	37.0	37.0	37.0
7	36.1825	37.0	37.0	37.0	37.0	37.0
8	36.817	37.0	37.0	37.0	37.0	37.0
9	35.9445	37.0	37.0	37.0	37.0	37.0
10	36.818	37.0	37.0	37.0	37.0	37.0
11	36.628	37.0	37.0	37.0	37.0	37.0
12	36.65	37.0	37.0	37.0	37.0	37.0
13	36.282	37.0	37.0	37.0	37.0	37.0
14	36.6095	37.0	37.0	37.0	37.0	37.0
15	36.5105	37.0	37.0	37.0	37.0	37.0
16	36.308	37.0	37.0	37.0	37.0	37.0
17	36.6225	37.0	37.0	37.0	37.0	37.0
18	36.8505	37.0	37.0	37.0	37.0	37.0
19	36.524	37.0	37.0	37.0	37.0	37.0
20	36.68	37.0	37.0	37.0	37.0	37.0
21	36.5585	37.0	37.0	37.0	37.0	37.0
22	36.69	37.0	37.0	37.0	37.0	37.0
23	36.5505	37.0	37.0	37.0	37.0	37.0
24	36.7155	37.0	37.0	37.0	37.0	37.0
25	36.464	37.0	37.0	37.0	37.0	37.0
26	36.4025	37.0	37.0	37.0	37.0	37.0
27	36.1015	37.0	37.0	37.0	37.0	37.0
28	36.022	37.0	37.0	37.0	37.0	37.0
29	35.6055	37.0	37.0	37.0	37.0	37.0
30	35.641	37.0	37.0	37.0	37.0	37.0
31	34.3725	37.0	37.0	37.0	25.0	37.0
32	35.083	37.0	37.0	37.0	37.0	37.0
33	35.2105	37.0	37.0	37.0	37.0	37.0
34	35.0745	37.0	37.0	37.0	25.0	37.0
35	34.417	37.0	37.0	37.0	25.0	37.0
36	34.733	37.0	37.0	37.0	25.0	37.0
37	34.0615	37.0	37.0	37.0	25.0	37.0
38	33.738	37.0	37.0	37.0	25.0	37.0
39	33.283	37.0	37.0	37.0	25.0	37.0
40	33.445	37.0	37.0	37.0	25.0	37.0
41	32.49	37.0	37.0	37.0	11.0	37.0
42	33.1035	37.0	37.0	37.0	25.0	37.0
43	32.843	37.0	37.0	37.0	11.0	37.0
44	32.773	37.0	37.0	37.0	11.0	37.0
45	31.513	37.0	25.0	37.0	11.0	37.0
46	32.3925	37.0	37.0	37.0	11.0	37.0
47	32.382	37.0	37.0	37.0	11.0	37.0
48	32.3315	37.0	25.0	37.0	11.0	37.0
49	30.57	37.0	25.0	37.0	11.0	37.0
50	32.0775	37.0	25.0	37.0	11.0	37.0
51	31.7185	37.0	25.0	37.0	11.0	37.0
52	32.1095	37.0	25.0	37.0	11.0	37.0
53	31.001	37.0	25.0	37.0	11.0	37.0
54	31.5355	37.0	25.0	37.0	11.0	37.0
55	31.175	37.0	25.0	37.0	11.0	37.0
56	31.528	37.0	25.0	37.0	11.0	37.0
57	30.943	37.0	25.0	37.0	11.0	37.0
58	31.8425	37.0	25.0	37.0	11.0	37.0
59	31.497	37.0	25.0	37.0	11.0	37.0
60	30.743	37.0	25.0	37.0	11.0	37.0
61	30.499	37.0	25.0	37.0	11.0	37.0
62	31.5845	37.0	25.0	37.0	11.0	37.0
63	31.5225	37.0	25.0	37.0	11.0	37.0
64	31.23	37.0	25.0	37.0	11.0	37.0
65	30.7255	37.0	25.0	37.0	11.0	37.0
66	30.9285	37.0	25.0	37.0	11.0	37.0
67	30.8055	37.0	25.0	37.0	11.0	37.0
68	31.0975	37.0	25.0	37.0	11.0	37.0
69	30.818	37.0	25.0	37.0	11.0	37.0
70	30.35	37.0	25.0	37.0	11.0	37.0
71	30.3775	37.0	25.0	37.0	11.0	37.0
72	29.917	37.0	25.0	37.0	11.0	37.0
73	28.833	37.0	25.0	37.0	11.0	37.0
74	30.186	37.0	25.0	37.0	11.0	37.0
75	29.242	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	10.0
24	32.0
25	33.0
26	55.0
27	88.0
28	111.0
29	140.0
30	158.0
31	235.0
32	292.0
33	433.0
34	606.0
35	978.0
36	791.0
37	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	73.425	14.625	2.25	9.700000000000001
2	64.525	11.65	9.075	14.75
3	40.8	16.150000000000002	19.3	23.75
4	47.675	12.25	12.7	27.375
5	27.975	34.675	11.675	25.674999999999997
6	34.35	34.75	10.575	20.325
7	54.474999999999994	19.25	6.0	20.275000000000002
8	37.875	28.1	5.375	28.65
9	57.025000000000006	16.1	5.6000000000000005	21.275
10	35.825	28.15	16.7	19.325
11	51.675000000000004	10.549999999999999	11.75	26.025
12	30.275000000000002	32.95	13.4	23.375
13	34.599999999999994	20.05	30.9	14.45
14	50.224999999999994	29.25	10.025	10.5
15	36.449999999999996	35.875	9.575	18.099999999999998
16	43.775	37.55	7.074999999999999	11.600000000000001
17	30.45	47.9	11.825	9.825000000000001
18	28.125	34.825	11.025	26.025
19	20.875	41.65	6.3	31.175000000000004
20	22.475	38.375	14.075	25.074999999999996
21	24.025	42.9	9.625	23.45
22	25.924999999999997	53.675	11.025	9.375
23	27.250000000000004	58.550000000000004	8.200000000000001	6.0
24	25.2	59.425	4.75	10.625
25	18.5	66.60000000000001	4.35	10.549999999999999
26	7.675	75.75	6.7250000000000005	9.85
27	12.775	74.225	6.125	6.875000000000001
28	8.200000000000001	77.575	6.675000000000001	7.55
29	13.725000000000001	72.625	4.2	9.45
30	13.900000000000002	73.5	7.875	4.725
31	13.65	72.925	7.225	6.2
32	17.8	71.025	5.35	5.825
33	15.5	70.89999999999999	5.825	7.775
34	13.350000000000001	69.425	10.549999999999999	6.675000000000001
35	15.45	68.7	3.6999999999999997	12.15
36	22.5	68.75	3.05	5.7
37	19.925	67.675	3.65	8.75
38	19.3	68.22500000000001	5.1	7.375
39	18.075	66.025	6.925000000000001	8.975
40	22.475	57.49999999999999	7.7	12.325
41	24.7	54.300000000000004	5.525	15.475
42	26.424999999999997	52.425	6.3	14.85
43	22.875	51.55	8.0	17.575
44	21.575	54.05	6.325	18.05
45	27.575	47.599999999999994	7.449999999999999	17.375
46	30.25	45.675	7.675	16.400000000000002
47	25.674999999999997	44.95	10.475	18.9
48	27.275	40.075	11.625	21.025
49	24.05	40.35	11.95	23.65
50	23.175	43.375	10.475	22.975
51	23.275000000000002	39.550000000000004	11.450000000000001	25.724999999999998
52	21.4	41.575	9.425	27.6
53	22.975	35.825	12.475	28.725
54	20.549999999999997	32.9	15.625	30.925000000000004
55	17.125	28.7	20.875	33.300000000000004
56	20.275000000000002	24.25	18.725	36.75
57	24.175	27.0	18.95	29.875
58	23.025000000000002	30.625000000000004	17.125	29.225
59	17.675	35.625	17.525	29.175
60	17.025000000000002	33.225	19.825	29.925
61	18.45	27.700000000000003	19.8	34.050000000000004
62	14.224999999999998	30.275000000000002	19.675	35.825
63	14.174999999999999	30.95	16.2	38.675
64	14.7	27.625	17.75	39.925
65	17.375	30.675	20.375	31.574999999999996
66	17.925	28.4	20.349999999999998	33.324999999999996
67	16.675	33.025	18.525	31.775
68	15.975	32.824999999999996	16.275000000000002	34.925
69	18.125	28.4	19.875	33.6
70	15.725	24.275	25.124999999999996	34.875
71	14.725	23.625	23.849999999999998	37.8
72	15.775	27.525	27.1	29.599999999999998
73	19.8	29.975	21.675	28.549999999999997
74	18.175	30.3	22.35	29.175
75	20.150000000000002	26.724999999999998	22.825	30.3
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	1.5
14	1.5
15	1.5
16	2.0
17	2.5
18	1.5
19	1.0
20	2.0
21	2.0
22	3.0
23	5.0
24	6.0
25	4.5
26	10.5
27	14.0
28	10.0
29	10.0
30	15.0
31	20.5
32	21.0
33	23.5
34	27.5
35	34.0
36	39.0
37	39.0
38	57.5
39	121.0
40	166.0
41	341.0
42	463.5
43	344.0
44	277.0
45	292.0
46	322.0
47	308.5
48	280.0
49	260.0
50	199.5
51	183.5
52	208.0
53	282.0
54	261.5
55	140.0
56	113.0
57	101.0
58	61.0
59	22.0
60	11.0
61	7.0
62	3.5
63	4.5
64	5.0
65	3.5
66	1.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.22890257153205	60.9
2	6.084751901484969	8.4
3	1.8833755885548715	3.9
4	1.0141253169141615	2.8000000000000003
5	0.796812749003984	2.75
6	0.3259688518652662	1.35
7	0.2535313292285404	1.225
8	0.21731256791017745	1.2
9	0.10865628395508872	0.675
>10	1.0141253169141615	13.25
>50	0.07243752263672583	3.55
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA	89	2.225	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATC	53	1.325	TruSeq Adapter, Index 19 (97% over 38bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	39	0.975	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	35	0.8750000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG	33	0.8250000000000001	TruSeq Adapter, Index 19 (97% over 38bp)
ACGGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATC	32	0.8	TruSeq Adapter, Index 19 (97% over 38bp)
GAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG	30	0.75	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTC	28	0.7000000000000001	TruSeq Adapter, Index 19 (97% over 38bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	24	0.6	TruSeq Adapter, Index 1 (97% over 35bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	23	0.575	TruSeq Adapter, Index 7 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA	22	0.5499999999999999	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG	19	0.475	TruSeq Adapter, Index 19 (97% over 38bp)
GAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC	19	0.475	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGC	17	0.42500000000000004	TruSeq Adapter, Index 19 (97% over 38bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCCCGGGTGGAGCCGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG	16	0.4	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA	15	0.375	TruSeq Adapter, Index 7 (97% over 37bp)
GGGCCCGGGTGGAGCCGCCGCAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	15	0.375	TruSeq Adapter, Index 1 (97% over 35bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTGAAGAGACATGAGAGGTGTAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	14	0.35000000000000003	TruSeq Adapter, Index 7 (97% over 36bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG	13	0.325	TruSeq Adapter, Index 19 (97% over 38bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	12	0.3	TruSeq Adapter, Index 7 (97% over 36bp)
ACGGTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTA	12	0.3	TruSeq Adapter, Index 19 (97% over 38bp)
GAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC	11	0.27499999999999997	TruSeq Adapter, Index 19 (97% over 38bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGAAGAGACATGAGAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC	10	0.25	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA	9	0.22499999999999998	TruSeq Adapter, Index 19 (97% over 38bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCG	8	0.2	TruSeq Adapter, Index 19 (97% over 38bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGCCCGGGTGGAGCCGCCGCAGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	8	0.2	TruSeq Adapter, Index 1 (97% over 35bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA	8	0.2	TruSeq Adapter, Index 7 (97% over 37bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAT	8	0.2	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT	7	0.17500000000000002	TruSeq Adapter, Index 19 (97% over 38bp)
GTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC	7	0.17500000000000002	TruSeq Adapter, Index 19 (97% over 38bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTCTTAGCGGTGGATCACTCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	7	0.17500000000000002	TruSeq Adapter, Index 1 (97% over 35bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCCGCGGGTGCGCGTCTCTCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	7	0.17500000000000002	TruSeq Adapter, Index 1 (97% over 35bp)
CCCCCGCCGGGCGTCGGGACCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	7	0.17500000000000002	TruSeq Adapter, Index 1 (97% over 35bp)
ACGGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA	6	0.15	TruSeq Adapter, Index 7 (97% over 37bp)
CCCCCGCCGGGCGTCGGGACCGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	6	0.15	TruSeq Adapter, Index 7 (97% over 36bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	6	0.15	TruSeq Adapter, Index 1 (97% over 35bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC	6	0.15	TruSeq Adapter, Index 19 (97% over 38bp)
GGGTTCCGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCG	6	0.15	TruSeq Adapter, Index 19 (97% over 38bp)
ACGGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA	5	0.125	TruSeq Adapter, Index 7 (97% over 37bp)
CCCCCGCCGGGCGTCGGGACCGGGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCCCGGGTGGAGCCGCCGCAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA	5	0.125	TruSeq Adapter, Index 7 (97% over 37bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGAAGCCGGCGGGAGCCCCGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	5	0.125	TruSeq Adapter, Index 1 (97% over 35bp)
CCCCCGCCGGGCGTCGGGACCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	5	0.125	TruSeq Adapter, Index 7 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
ATCAGAAGATTGCAGGTTCGGGTCCTGCCGCGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGAAGAGACATGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCG	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
GTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCT	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGCGGCGGGTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCG	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
GAGCGTACGCGTTAGGACCCGAAAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	5	0.125	TruSeq Adapter, Index 1 (97% over 35bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCCACGGCGCGACCGCTCTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
ACGGTGAAGAGACATGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAT	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCG	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCT	5	0.125	TruSeq Adapter, Index 19 (97% over 38bp)
ATCAGAAGATTCCAGGTTCGGCTCCTGGCTGGCTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTCGGCGCGCGGCGCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA	5	0.125	TruSeq Adapter, Index 7 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.025	0.0	0.0	0.0	0.0
15	0.05	0.0	0.0	0.0	0.0
16	0.1	0.0	0.0	0.0	0.0
17	0.125	0.0	0.0	0.0	0.0
18	0.25	0.0	0.0	0.0	0.0
19	0.3	0.0	0.0	0.0	0.0
20	0.375	0.0	0.0	0.0	0.0
21	0.675	0.0	0.0	0.0	0.0
22	0.975	0.0	0.0	0.0	0.0
23	1.35	0.0	0.0	0.0	0.0
24	1.975	0.0	0.0	0.0	0.0
25	3.0	0.0	0.0	0.0	0.0
26	4.3	0.0	0.0	0.0	0.0
27	6.2	0.0	0.0	0.0	0.0
28	9.3	0.0	0.0	0.0	0.0
29	12.925	0.0	0.0	0.0	0.0
30	16.725	0.0	0.0	0.0	0.0
31	21.35	0.0	0.0	0.0	0.0
32	23.775	0.0	0.0	0.0	0.0
33	25.75	0.0	0.0	0.0	0.0
34	28.35	0.0	0.0	0.0	0.0
35	32.375	0.0	0.0	0.0	0.0
36	37.75	0.0	0.0	0.0	0.0
37	44.35	0.0	0.0	0.0	0.0
38	48.175	0.0	0.0	0.0	0.0
39	52.65	0.0	0.0	0.0	0.0
40	58.0	0.0	0.0	0.0	0.0
41	61.4	0.0	0.0	0.0	0.0
42	64.35	0.0	0.0	0.0	0.0
43	66.375	0.0	0.0	0.0	0.0
44	69.4	0.0	0.0	0.0	0.0
45	72.15	0.0	0.0	0.0	0.0
46	73.425	0.0	0.0	0.0	0.0
47	74.875	0.0	0.0	0.0	0.0
48	76.175	0.0	0.0	0.0	0.0
49	77.125	0.0	0.0	0.0	0.0
50	78.45	0.0	0.0	0.0	0.0
51	79.925	0.0	0.0	0.0	0.0
52	82.55	0.0	0.0	0.0	0.0
53	83.6	0.0	0.0	0.0	0.0
54	83.9	0.0	0.0	0.0	0.0
55	84.05	0.0	0.0	0.0	0.0
56	84.175	0.0	0.0	0.0	0.0
57	84.175	0.0	0.0	0.0	0.0
58	84.175	0.0	0.0	0.0	0.0
59	84.2	0.0	0.0	0.0	0.0
60	84.2	0.0	0.0	0.0	0.0
61	84.2	0.0	0.0	0.0	0.0
62	84.225	0.0	0.0	0.0	0.0
63	84.225	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGGGT	15	0.0021872367	69.00001	4
GTCGCAA	15	0.0021872367	69.00001	33
CCGCCGC	15	0.0021872367	69.00001	15
GGTTCGT	15	0.0021872367	69.00001	15
TGGAGCC	15	0.0021872367	69.00001	10
CGGGTGG	15	0.0021872367	69.00001	6
AAGATTG	15	0.0021872367	69.00001	6
CGGTGAA	30	8.704228E-8	69.00001	2
AGGTTCG	15	0.0021872367	69.00001	14
CAGGTTC	15	0.0021872367	69.00001	13
GGTGGAG	15	0.0021872367	69.00001	8
GCCGCCG	15	0.0021872367	69.00001	14
GCCCGGG	15	0.0021872367	69.00001	3
ACGGTGA	30	8.704228E-8	69.00001	1
GTGGAGC	15	0.0021872367	69.00001	9
GGGTGGA	15	0.0021872367	69.00001	7
GGAGCCG	15	0.0021872367	69.00001	11
CGCCGCA	15	0.0021872367	69.00001	16
GGTCGCA	15	0.0021872367	69.00001	32
GGGTTCG	20	7.5213684E-5	69.0	14
>>END_MODULE
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036267 spots for SRR24920653.sra
Written 1036267 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
Read 1036264 spots for SRR24920653.sra
Written 1036264 spots for SRR24920653.sra
SRR ids: ['SRR24920653.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_3sbd8qbt
SRR24920653.sra spots: 16580227
blocks: [[1, 1036264], [1036265, 2072528], [2072529, 3108792], [3108793, 4145056], [4145057, 5181320], [5181321, 6217584], [6217585, 7253848], [7253849, 8290112], [8290113, 9326376], [9326377, 10362640], [10362641, 11398904], [11398905, 12435168], [12435169, 13471432], [13471433, 14507696], [14507697, 15543960], [15543961, 16580227]]
SRR24920653 file size 883681
SRR24920653 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24920653 SRR24920653_1.fastq
Input file:	SRR24920653_1.fastq
trimmed:	SRR24920653-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Thu Jul  2 04:51:06 2026 >> started

Thu Jul  2 04:51:18 2026 >> done (12.360s)
16580227 reads processed; of these:
   20075 ( 0.12%) short reads filtered out after trimming by size control
       4 ( 0.00%) empty reads filtered out after trimming by size control
16560148 (99.88%) reads available; of these:
12414933 (74.97%) trimmed reads available after processing
 4145215 (25.03%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   13862	  0.08%
 19	   24592	  0.15%
 20	   41852	  0.25%
 21	   53480	  0.32%
 22	   66164	  0.40%
 23	   88358	  0.53%
 24	  146341	  0.88%
 25	  249911	  1.51%
 26	  330485	  2.00%
 27	  482318	  2.91%
 28	  697306	  4.21%
 29	  629710	  3.80%
 30	  831972	  5.02%
 31	  431603	  2.61%
 32	  418189	  2.53%
 33	  468955	  2.83%
 34	  712372	  4.30%
 35	 1042494	  6.30%
 36	 1100341	  6.64%
 37	  685976	  4.14%
 38	  927649	  5.60%
 39	  912026	  5.51%
 40	  569257	  3.44%
 41	  563683	  3.40%
 42	  415545	  2.51%
 43	  510463	  3.08%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       0	  0.00%
 69	       0	  0.00%
 70	       0	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       0	  0.00%
 74	      29	  0.00%
 75	 4145215	 25.03%
16560148 reads passed initial QC


criterion=sequence-density
sequence-density=3.19
sequence-density-rank=1
fanout-score=3.03
fanout-score-rank=7
prefix-density=3.19
prefix-fanout=3.0
sequence=GTCCCATCTGGGTCGCCAAAAAAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=33.24
fanout-score-rank=1
prefix-density=0.20
prefix-fanout=3.0
sequence=CGGGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x GTCCCATCTGGGTCGCCAAAAAAAAA -o SRR24920653 -
Input file:	STDIN
trimmed:	SRR24920653-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCCCATCTGGGTCGCCAAAAAAAAA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	16
Thu Jul  2 04:51:47 2026 >> started

Thu Jul  2 04:51:54 2026 >> done (6.651s)
8280074 reads processed; of these:
   1625 ( 0.02%) short reads filtered out after trimming by size control
    431 ( 0.01%) empty reads filtered out after trimming by size control
8278018 (99.98%) reads available; of these:
 198060 ( 2.39%) trimmed reads available after processing
8079958 (97.61%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   7162	  0.09%
 19	  27398	  0.33%
 20	  25501	  0.31%
 21	 169551	  2.05%
 22	  41647	  0.50%
 23	  46478	  0.56%
 24	  73448	  0.89%
 25	 124793	  1.51%
 26	 165001	  1.99%
 27	 240482	  2.91%
 28	 349532	  4.22%
 29	 314714	  3.80%
 30	 415624	  5.02%
 31	 214905	  2.60%
 32	 208860	  2.52%
 33	 234600	  2.83%
 34	 356275	  4.30%
 35	 520948	  6.29%
 36	 550899	  6.65%
 37	 343240	  4.15%
 38	 462482	  5.59%
 39	 456148	  5.51%
 40	 284245	  3.43%
 41	 281738	  3.40%
 42	 207783	  2.51%
 43	 255275	  3.08%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      1	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	     38	  0.00%
 68	    520	  0.01%
 69	     23	  0.00%
 70	    419	  0.01%
 71	    432	  0.01%
 72	  23347	  0.28%
 73	      0	  0.00%
 74	     16	  0.00%
 75	1874493	 22.64%


criterion=sequence-density
sequence-density=2.04
sequence-density-rank=1
fanout-score=10.04
fanout-score-rank=2
prefix-density=0.01
prefix-fanout=10.0
sequence=AAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=31.30
fanout-score-rank=1
prefix-density=0.20
prefix-fanout=2.9
sequence=CGGGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC
                                 Started job on |	Jul 02 04:52:56
                             Started mapping on |	Jul 02 04:52:57
                                    Finished on |	Jul 02 04:54:30
       Mapping speed, Million of reads per hour |	640.96

                          Number of input reads |	16558092
                      Average input read length |	39
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3871871
                        Uniquely mapped reads % |	23.38%
                          Average mapped length |	24.50
                       Number of splices: Total |	316286
            Number of splices: Annotated (sjdb) |	965
                       Number of splices: GT/AG |	252587
                       Number of splices: GC/AG |	12324
                       Number of splices: AT/AC |	121
               Number of splices: Non-canonical |	51254
                      Mismatch rate per base, % |	0.92%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.93
                        Insertion rate per base |	0.00%
                       Insertion average length |	2.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5265300
             % of reads mapped to multiple loci |	31.80%
        Number of reads mapped to too many loci |	598020
             % of reads mapped to too many loci |	3.61%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	39.53%
                     % of reads unmapped: other |	1.67%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	7420921	7420921	7420921
N_multimapping	5265300	5265300	5265300
N_noFeature	3626679	3745217	3744948
N_ambiguous	10619	2019	4793
UnstrandedReadsAssigned:234573 PositiveStrandReadsAssigned:124635 NegativeStrandReadsAssigned:122130
Dataset is classified unstranded
MeadianReadLen=33 20thPercentileLength=26 echo kmer=21
SRR24920653 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=21

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 21
[index] number of targets: 109,282
[index] number of k-mers: 93,655,514
[index] number of equivalence classes: 565,482
[quant] running in single-end mode
[quant] will process file 1: SRR24920653-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 16,558,092 reads, 2,451,905 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,261 rounds

 109283 SRR24920653.ke.tsv
  52636 SRR24920653.se.tsv
 161919 total
==> SRR24920653.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR24920653.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24920653 completed mapping pipeline successfully
