Starting /dee2/code/volunteer_pipeline.sh SRR24920647
    current disk space = 443981672448
    free memory = 333066188 
SRR24920647 SRAfilesize
3dd9e235fe510863624be7e5f0cab2fd  SRR24920647.sra
SRR24920647.sra file validated
SRR24920647 is single end
SRR24920647 is conventional basespace
SRR24920647 read1 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24920647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.275	37.0	37.0	37.0	37.0	37.0
2	36.747	37.0	37.0	37.0	37.0	37.0
3	35.9005	37.0	37.0	37.0	37.0	37.0
4	36.773	37.0	37.0	37.0	37.0	37.0
5	36.6195	37.0	37.0	37.0	37.0	37.0
6	36.667	37.0	37.0	37.0	37.0	37.0
7	36.142	37.0	37.0	37.0	37.0	37.0
8	36.8	37.0	37.0	37.0	37.0	37.0
9	35.946	37.0	37.0	37.0	37.0	37.0
10	36.816	37.0	37.0	37.0	37.0	37.0
11	36.6825	37.0	37.0	37.0	37.0	37.0
12	36.6045	37.0	37.0	37.0	37.0	37.0
13	36.3425	37.0	37.0	37.0	37.0	37.0
14	36.7395	37.0	37.0	37.0	37.0	37.0
15	36.547	37.0	37.0	37.0	37.0	37.0
16	36.028	37.0	37.0	37.0	37.0	37.0
17	36.5615	37.0	37.0	37.0	37.0	37.0
18	36.826	37.0	37.0	37.0	37.0	37.0
19	36.5515	37.0	37.0	37.0	37.0	37.0
20	36.7	37.0	37.0	37.0	37.0	37.0
21	36.579	37.0	37.0	37.0	37.0	37.0
22	36.769	37.0	37.0	37.0	37.0	37.0
23	36.5865	37.0	37.0	37.0	37.0	37.0
24	36.768	37.0	37.0	37.0	37.0	37.0
25	36.5475	37.0	37.0	37.0	37.0	37.0
26	36.55	37.0	37.0	37.0	37.0	37.0
27	36.297	37.0	37.0	37.0	37.0	37.0
28	36.1905	37.0	37.0	37.0	37.0	37.0
29	35.783	37.0	37.0	37.0	37.0	37.0
30	35.6865	37.0	37.0	37.0	37.0	37.0
31	34.6365	37.0	37.0	37.0	25.0	37.0
32	35.3165	37.0	37.0	37.0	37.0	37.0
33	35.4985	37.0	37.0	37.0	37.0	37.0
34	35.536	37.0	37.0	37.0	37.0	37.0
35	34.969	37.0	37.0	37.0	25.0	37.0
36	35.253	37.0	37.0	37.0	37.0	37.0
37	34.455	37.0	37.0	37.0	25.0	37.0
38	33.86	37.0	37.0	37.0	25.0	37.0
39	33.6115	37.0	37.0	37.0	25.0	37.0
40	33.842	37.0	37.0	37.0	25.0	37.0
41	33.2805	37.0	37.0	37.0	25.0	37.0
42	33.8245	37.0	37.0	37.0	25.0	37.0
43	33.44	37.0	37.0	37.0	25.0	37.0
44	33.3515	37.0	37.0	37.0	25.0	37.0
45	31.9435	37.0	25.0	37.0	11.0	37.0
46	32.6985	37.0	37.0	37.0	11.0	37.0
47	32.22	37.0	37.0	37.0	11.0	37.0
48	32.4725	37.0	37.0	37.0	11.0	37.0
49	30.928	37.0	25.0	37.0	11.0	37.0
50	32.484	37.0	37.0	37.0	11.0	37.0
51	32.191	37.0	25.0	37.0	11.0	37.0
52	32.1515	37.0	25.0	37.0	11.0	37.0
53	31.1395	37.0	25.0	37.0	11.0	37.0
54	31.618	37.0	25.0	37.0	11.0	37.0
55	31.367	37.0	25.0	37.0	11.0	37.0
56	31.57	37.0	25.0	37.0	11.0	37.0
57	31.0915	37.0	25.0	37.0	11.0	37.0
58	31.6555	37.0	25.0	37.0	11.0	37.0
59	31.595	37.0	25.0	37.0	11.0	37.0
60	31.4095	37.0	25.0	37.0	11.0	37.0
61	30.4535	37.0	25.0	37.0	11.0	37.0
62	31.3915	37.0	25.0	37.0	11.0	37.0
63	31.767	37.0	25.0	37.0	11.0	37.0
64	31.8415	37.0	25.0	37.0	11.0	37.0
65	30.5855	37.0	25.0	37.0	11.0	37.0
66	30.7715	37.0	25.0	37.0	11.0	37.0
67	30.584	37.0	25.0	37.0	11.0	37.0
68	30.726	37.0	25.0	37.0	11.0	37.0
69	30.3925	37.0	25.0	37.0	11.0	37.0
70	30.5235	37.0	25.0	37.0	11.0	37.0
71	30.5575	37.0	25.0	37.0	11.0	37.0
72	29.7165	37.0	25.0	37.0	11.0	37.0
73	28.7875	37.0	25.0	37.0	11.0	37.0
74	29.699	37.0	25.0	37.0	11.0	37.0
75	28.6185	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	13.0
24	22.0
25	23.0
26	67.0
27	99.0
28	115.0
29	126.0
30	118.0
31	226.0
32	282.0
33	432.0
34	604.0
35	975.0
36	854.0
37	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	75.2	9.8	1.55	13.450000000000001
2	70.35	8.325000000000001	5.800000000000001	15.525
3	33.25	15.825	23.974999999999998	26.950000000000003
4	51.225	10.525	10.6	27.650000000000002
5	24.349999999999998	42.825	6.0249999999999995	26.8
6	35.225	38.975	7.074999999999999	18.725
7	62.275000000000006	11.625	3.4000000000000004	22.7
8	36.3	27.900000000000002	3.05	32.75
9	59.425	13.725000000000001	3.45	23.400000000000002
10	39.625	28.175	15.425	16.775000000000002
11	49.3	7.775	12.125	30.8
12	29.375	30.475	10.85	29.299999999999997
13	41.625	13.55	34.425	10.4
14	58.699999999999996	21.5	10.4	9.4
15	38.9	38.775	7.625	14.7
16	47.0	35.525	8.674999999999999	8.799999999999999
17	33.225	44.224999999999994	10.549999999999999	12.0
18	35.15	28.975	11.675	24.2
19	24.725	36.475	6.550000000000001	32.25
20	21.675	31.674999999999997	18.85	27.800000000000004
21	27.950000000000003	35.35	9.2	27.500000000000004
22	32.25	44.7	12.1	10.95
23	32.5	53.825	7.3	6.375
24	31.7	54.85	3.05	10.4
25	23.9	61.525	3.925	10.65
26	8.175	72.275	8.0	11.55
27	14.549999999999999	69.65	6.125	9.675
28	9.25	73.3	7.449999999999999	10.0
29	17.299999999999997	69.625	3.925	9.15
30	15.125	70.825	9.675	4.375
31	13.775	72.25	7.825	6.15
32	18.35	70.42500000000001	6.0249999999999995	5.2
33	14.549999999999999	72.0	4.775	8.674999999999999
34	12.075	72.52499999999999	9.875	5.525
35	13.475000000000001	72.15	3.05	11.325000000000001
36	20.075000000000003	73.5	2.375	4.05
37	17.95	71.3	3.65	7.1
38	18.975	70.075	4.7	6.25
39	15.0	69.27499999999999	7.5249999999999995	8.200000000000001
40	20.0	59.575	8.7	11.725
41	22.325	56.99999999999999	4.75	15.925
42	25.3	58.45	4.6	11.65
43	22.975	55.474999999999994	6.375	15.174999999999999
44	19.525000000000002	61.3	4.9	14.274999999999999
45	26.900000000000002	50.675000000000004	7.95	14.475
46	29.675	48.1	7.175	15.049999999999999
47	26.0	45.7	11.825	16.475
48	29.299999999999997	39.5	9.825000000000001	21.375
49	24.775	41.725	12.025	21.475
50	23.025000000000002	46.625	8.375	21.975
51	23.775	43.075	9.35	23.799999999999997
52	22.400000000000002	44.275	8.649999999999999	24.675
53	26.0	36.475	10.45	27.075
54	22.900000000000002	32.45	16.400000000000002	28.249999999999996
55	18.675	30.099999999999998	19.625	31.6
56	20.375	25.4	18.025	36.199999999999996
57	25.900000000000002	30.0	17.625	26.474999999999998
58	24.3	32.725	14.75	28.225
59	18.575	39.225	15.375	26.825
60	16.725	33.25	18.75	31.275
61	17.95	28.675	20.0	33.375
62	13.700000000000001	29.4	22.7	34.2
63	13.775	31.65	18.325	36.25
64	12.675	30.075000000000003	21.825	35.425000000000004
65	16.35	33.550000000000004	23.075000000000003	27.025
66	15.5	29.549999999999997	24.7	30.25
67	10.95	28.425	25.275	35.35
68	11.85	31.924999999999997	19.575	36.65
69	14.499999999999998	28.325	20.599999999999998	36.575
70	11.774999999999999	28.4	26.1	33.725
71	11.65	30.625000000000004	25.85	31.874999999999996
72	13.025	27.750000000000004	32.324999999999996	26.900000000000002
73	13.750000000000002	29.45	27.775	29.025000000000002
74	11.899999999999999	31.225	27.075	29.799999999999997
75	11.85	22.85	28.625	36.675000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	1.0
16	2.0
17	1.0
18	1.5
19	2.5
20	2.0
21	2.5
22	3.5
23	4.0
24	4.0
25	5.0
26	9.0
27	11.0
28	10.0
29	7.5
30	13.0
31	21.0
32	21.0
33	20.5
34	25.0
35	34.5
36	39.0
37	54.5
38	152.5
39	420.5
40	606.0
41	445.0
42	239.0
43	194.0
44	194.0
45	201.5
46	228.5
47	240.0
48	232.0
49	200.0
50	186.0
51	257.0
52	310.0
53	324.5
54	241.0
55	131.5
56	120.0
57	124.0
58	107.5
59	55.0
60	23.0
61	16.5
62	8.5
63	6.0
64	5.0
65	3.0
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.650000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25304136253041	53.175
2	6.89375506893755	8.5
3	2.3114355231143553	4.275
4	1.05433901054339	2.6
5	0.6082725060827251	1.875
6	0.48661800486618007	1.7999999999999998
7	0.527169505271695	2.275
8	0.28386050283860503	1.4000000000000001
9	0.32441200324412006	1.7999999999999998
>10	1.05433901054339	12.55
>50	0.16220600162206003	6.15
>100	0.04055150040551501	3.5999999999999996
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC	144	3.5999999999999996	TruSeq Adapter, Index 1 (97% over 38bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	80	2.0	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	58	1.4500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATC	54	1.35	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	54	1.35	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTC	38	0.95	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	35	0.8750000000000001	TruSeq Adapter, Index 27 (97% over 39bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC	34	0.8500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	32	0.8	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA	29	0.7250000000000001	TruSeq Adapter, Index 1 (97% over 37bp)
GGTGAAGAGACATGAGAGGTGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC	26	0.65	TruSeq Adapter, Index 1 (97% over 38bp)
GGTGAAGAGACATGAGAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	25	0.625	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG	23	0.575	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGC	21	0.525	TruSeq Adapter, Index 27 (97% over 39bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	19	0.475	TruSeq Adapter, Index 8 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	19	0.475	TruSeq Adapter, Index 1 (100% over 35bp)
ACGGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATC	18	0.44999999999999996	TruSeq Adapter, Index 27 (97% over 39bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	15	0.375	TruSeq Adapter, Index 27 (97% over 39bp)
GGCCGCGGGTGCGCGTCTCTCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	14	0.35000000000000003	TruSeq Adapter, Index 1 (100% over 35bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTGAAGAGACATGAGAGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	11	0.27499999999999997	TruSeq Adapter, Index 27 (97% over 39bp)
GGGCGGCGGGTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGT	11	0.27499999999999997	TruSeq Adapter, Index 27 (97% over 39bp)
GGCCGCGGGTGCGCGTCTCTCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	10	0.25	TruSeq Adapter, Index 8 (97% over 36bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCCCGGGTGGAGCCGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	10	0.25	TruSeq Adapter, Index 27 (97% over 39bp)
CCCCCGCCGGGCGTCGGGACCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	10	0.25	TruSeq Adapter, Index 1 (100% over 35bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	9	0.22499999999999998	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	9	0.22499999999999998	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCT	9	0.22499999999999998	TruSeq Adapter, Index 27 (97% over 39bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCG	9	0.22499999999999998	TruSeq Adapter, Index 27 (97% over 39bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	8	0.2	TruSeq Adapter, Index 8 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	8	0.2	TruSeq Adapter, Index 1 (100% over 35bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAT	8	0.2	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCG	8	0.2	TruSeq Adapter, Index 27 (97% over 39bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGAAGAGACATGAGAGGTGTAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	8	0.2	TruSeq Adapter, Index 8 (97% over 36bp)
GTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	7	0.17500000000000002	TruSeq Adapter, Index 27 (97% over 39bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGTCGGCGCGCGGCGCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA	7	0.17500000000000002	TruSeq Adapter, Index 1 (97% over 37bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCT	7	0.17500000000000002	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCG	7	0.17500000000000002	TruSeq Adapter, Index 27 (97% over 39bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCCGCCGGTGAAATACCACTACTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	7	0.17500000000000002	TruSeq Adapter, Index 1 (100% over 35bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC	7	0.17500000000000002	TruSeq Adapter, Index 1 (97% over 38bp)
GGGCTGGGGCGCGAAGCGGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC	7	0.17500000000000002	TruSeq Adapter, Index 1 (97% over 38bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	7	0.17500000000000002	TruSeq Adapter, Index 1 (100% over 35bp)
GGTGAAGAGACATGAGAGGTGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	6	0.15	TruSeq Adapter, Index 27 (97% over 39bp)
GTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	6	0.15	TruSeq Adapter, Index 27 (97% over 39bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCCGGGTGGAGCCGCCGCAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	6	0.15	TruSeq Adapter, Index 1 (100% over 35bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGA	6	0.15	TruSeq Adapter, Index 27 (97% over 39bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCCCGGGCCGCGGTTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATC	6	0.15	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGA	6	0.15	TruSeq Adapter, Index 27 (97% over 39bp)
GGGGCGGCGGGTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCG	6	0.15	TruSeq Adapter, Index 27 (97% over 39bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGAGCCCGGCGGGTGCCGGCGCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAAGGCCCGGTGGGGAAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACT	5	0.125	TruSeq Adapter, Index 27 (97% over 39bp)
GAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG	5	0.125	TruSeq Adapter, Index 27 (97% over 39bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGAACAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCG	5	0.125	TruSeq Adapter, Index 27 (97% over 39bp)
GGGCCCGGGTGGAGCCGCCGCAGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	5	0.125	TruSeq Adapter, Index 1 (100% over 35bp)
GTGAAGAGACATGAGAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCG	5	0.125	TruSeq Adapter, Index 27 (97% over 39bp)
GGTGAAGAGACATGAGAGGTGTAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA	5	0.125	TruSeq Adapter, Index 1 (97% over 37bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC	5	0.125	TruSeq Adapter, Index 27 (97% over 39bp)
GGGGCGGCGGGTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCG	5	0.125	TruSeq Adapter, Index 27 (97% over 39bp)
GTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC	5	0.125	TruSeq Adapter, Index 1 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.125	0.0	0.0	0.0	0.0
21	0.225	0.0	0.0	0.0	0.0
22	0.275	0.0	0.0	0.0	0.0
23	0.45	0.0	0.0	0.0	0.0
24	0.675	0.0	0.0	0.0	0.0
25	1.175	0.0	0.0	0.0	0.0
26	2.0	0.0	0.0	0.0	0.0
27	4.0	0.0	0.0	0.0	0.0
28	6.325	0.0	0.0	0.0	0.0
29	10.15	0.0	0.0	0.0	0.0
30	13.075	0.0	0.0	0.0	0.0
31	16.9	0.0	0.0	0.0	0.0
32	18.65	0.0	0.0	0.0	0.0
33	19.875	0.0	0.0	0.0	0.0
34	22.275	0.0	0.0	0.0	0.0
35	25.9	0.0	0.0	0.0	0.0
36	32.0	0.0	0.0	0.0	0.0
37	39.475	0.0	0.0	0.0	0.0
38	42.925	0.0	0.0	0.0	0.0
39	46.525	0.0	0.0	0.0	0.0
40	51.175	0.0	0.0	0.0	0.0
41	54.125	0.0	0.0	0.0	0.0
42	58.275	0.0	0.0	0.0	0.0
43	61.025	0.0	0.0	0.0	0.0
44	65.425	0.0	0.0	0.0	0.0
45	68.725	0.0	0.0	0.0	0.0
46	70.825	0.0	0.0	0.0	0.0
47	72.65	0.0	0.0	0.0	0.0
48	74.675	0.0	0.0	0.0	0.0
49	76.025	0.0	0.0	0.0	0.0
50	77.4	0.0	0.0	0.0	0.0
51	79.1	0.0	0.0	0.0	0.0
52	81.4	0.0	0.0	0.0	0.0
53	82.675	0.0	0.0	0.0	0.0
54	83.0	0.0	0.0	0.0	0.0
55	83.575	0.0	0.0	0.0	0.0
56	83.95	0.0	0.0	0.0	0.0
57	84.1	0.0	0.0	0.0	0.0
58	84.35	0.0	0.0	0.0	0.0
59	84.4	0.0	0.0	0.0	0.0
60	84.475	0.0	0.0	0.0	0.0
61	84.575	0.0	0.0	0.0	0.0
62	84.6	0.0	0.0	0.0	0.0
63	84.65	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCATC	30	8.704228E-8	69.00001	23
GTGTCCC	15	0.0021872367	69.00001	20
GGGTTCG	30	8.704228E-8	69.00001	14
TTCGTGT	15	0.0021872367	69.00001	17
CTGGGTC	30	8.704228E-8	69.00001	29
CTGGGGA	30	8.704228E-8	69.00001	3
TGTCCCA	15	0.0021872367	69.00001	21
TCTGGGT	30	8.704228E-8	69.00001	28
CATCTGG	30	8.704228E-8	69.00001	26
GCTGGGG	30	8.704228E-8	69.00001	2
GGATTGT	30	8.704228E-8	69.00001	7
GGTTCGT	15	0.0021872367	69.00001	15
TCGTGTC	15	0.0021872367	69.00001	18
CCCATCT	30	8.704228E-8	69.00001	24
GATTGTG	30	8.704228E-8	69.00001	8
GGGAGTA	15	0.0021872367	69.00001	23
GGGAGAA	15	0.0021872367	69.00001	23
GTGGGTT	30	8.704228E-8	69.00001	12
TGGGGAT	30	8.704228E-8	69.00001	4
GTCCCAT	30	8.704228E-8	69.00001	22
>>END_MODULE
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848304 spots for SRR24920647.sra
Written 848304 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
Read 848291 spots for SRR24920647.sra
Written 848291 spots for SRR24920647.sra
SRR ids: ['SRR24920647.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7l1iin_x
SRR24920647.sra spots: 13572669
blocks: [[1, 848291], [848292, 1696582], [1696583, 2544873], [2544874, 3393164], [3393165, 4241455], [4241456, 5089746], [5089747, 5938037], [5938038, 6786328], [6786329, 7634619], [7634620, 8482910], [8482911, 9331201], [9331202, 10179492], [10179493, 11027783], [11027784, 11876074], [11876075, 12724365], [12724366, 13572669]]
SRR24920647 file size 821566
SRR24920647 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR24920647 SRR24920647_1.fastq
Input file:	SRR24920647_1.fastq
trimmed:	SRR24920647-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Wed Jul  1 20:49:59 2026 >> started

Wed Jul  1 20:50:09 2026 >> done (9.780s)
13572669 reads processed; of these:
   10225 ( 0.08%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
13562444 (99.92%) reads available; of these:
 9410604 (69.39%) trimmed reads available after processing
 4151840 (30.61%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    6906	  0.05%
 19	   11282	  0.08%
 20	   15775	  0.12%
 21	   20388	  0.15%
 22	   28810	  0.21%
 23	   43107	  0.32%
 24	   78556	  0.58%
 25	  149656	  1.10%
 26	  248510	  1.83%
 27	  382623	  2.82%
 28	  524178	  3.86%
 29	  386016	  2.85%
 30	  519748	  3.83%
 31	  233275	  1.72%
 32	  196232	  1.45%
 33	  292496	  2.16%
 34	  522623	  3.85%
 35	  841299	  6.20%
 36	 1117160	  8.24%
 37	  508940	  3.75%
 38	  598429	  4.41%
 39	  632575	  4.66%
 40	  424641	  3.13%
 41	  541846	  4.00%
 42	  466118	  3.44%
 43	  619394	  4.57%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       0	  0.00%
 69	       0	  0.00%
 70	       0	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       0	  0.00%
 74	      21	  0.00%
 75	 4151840	 30.61%
13562444 reads passed initial QC


criterion=sequence-density
sequence-density=4.79
sequence-density-rank=1
fanout-score=2.91
fanout-score-rank=10
prefix-density=4.79
prefix-fanout=2.9
sequence=GTCCCATCTGGGTCGCC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=344.72
fanout-score-rank=1
prefix-density=2.77
prefix-fanout=1.3
sequence=GGGGGGCGGCGGGTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x GTCCCATCTGGGTCGCC -o SRR24920647 -
Input file:	STDIN
trimmed:	SRR24920647-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCCCATCTGGGTCGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	16
Wed Jul  1 20:50:42 2026 >> started

Wed Jul  1 20:50:49 2026 >> done (7.639s)
8137467 reads processed; of these:
   1603 ( 0.02%) short reads filtered out after trimming by size control
    448 ( 0.01%) empty reads filtered out after trimming by size control
8135416 (99.97%) reads available; of these:
 326366 ( 4.01%) trimmed reads available after processing
7809050 (95.99%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   4287	  0.05%
 19	  25541	  0.31%
 20	  15728	  0.19%
 21	 234850	  2.89%
 22	  32800	  0.40%
 23	  30366	  0.37%
 24	  47143	  0.58%
 25	  89851	  1.10%
 26	 149456	  1.84%
 27	 228803	  2.81%
 28	 314565	  3.87%
 29	 232115	  2.85%
 30	 311688	  3.83%
 31	 139899	  1.72%
 32	 117751	  1.45%
 33	 174816	  2.15%
 34	 313511	  3.85%
 35	 505227	  6.21%
 36	 670711	  8.24%
 37	 304955	  3.75%
 38	 359076	  4.41%
 39	 379185	  4.66%
 40	 254169	  3.12%
 41	 325382	  4.00%
 42	 279440	  3.43%
 43	 371856	  4.57%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      1	  0.00%
 64	      1	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	     97	  0.00%
 68	   2190	  0.03%
 69	    292	  0.00%
 70	    499	  0.01%
 71	    783	  0.01%
 72	  54708	  0.67%
 73	      0	  0.00%
 74	     10	  0.00%
 75	2163664	 26.60%


criterion=sequence-density
sequence-density=2.24
sequence-density-rank=1
fanout-score=13.75
fanout-score-rank=5
prefix-density=0.01
prefix-fanout=13.7
sequence=AAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=312.07
fanout-score-rank=1
prefix-density=2.78
prefix-fanout=1.3
sequence=GGGGGGCGGCGGGTC
                                 Started job on |	Jul 01 20:51:47
                             Started mapping on |	Jul 01 20:51:47
                                    Finished on |	Jul 01 20:53:21
       Mapping speed, Million of reads per hour |	519.33

                          Number of input reads |	13560393
                      Average input read length |	42
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2797183
                        Uniquely mapped reads % |	20.63%
                          Average mapped length |	24.66
                       Number of splices: Total |	256099
            Number of splices: Annotated (sjdb) |	462
                       Number of splices: GT/AG |	222998
                       Number of splices: GC/AG |	9467
                       Number of splices: AT/AC |	73
               Number of splices: Non-canonical |	23561
                      Mismatch rate per base, % |	1.04%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.57
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.83
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4299482
             % of reads mapped to multiple loci |	31.71%
        Number of reads mapped to too many loci |	291968
             % of reads mapped to too many loci |	2.15%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	44.65%
                     % of reads unmapped: other |	0.86%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6463728	6463728	6463728
N_multimapping	4299482	4299482	4299482
N_noFeature	2603454	2693879	2703961
N_ambiguous	5991	3128	1597
UnstrandedReadsAssigned:187738 PositiveStrandReadsAssigned:100176 NegativeStrandReadsAssigned:91625
Dataset is classified unstranded
MeadianReadLen=34 20thPercentileLength=26 echo kmer=21
SRR24920647 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=21

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 21
[index] number of targets: 109,282
[index] number of k-mers: 93,655,514
[index] number of equivalence classes: 565,482
[quant] running in single-end mode
[quant] will process file 1: SRR24920647-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,560,393 reads, 2,075,271 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,195 rounds

 109283 SRR24920647.ke.tsv
  52636 SRR24920647.se.tsv
 161919 total
==> SRR24920647.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR24920647.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24920647 completed mapping pipeline successfully
