Starting /dee2/code/volunteer_pipeline.sh SRR24920652
    current disk space = 1181637280
    free memory = 197167116 
SRR24920652 SRAfilesize
e4d73a971129f5a06e9dee42be427608  SRR24920652.sra
SRR24920652.sra file validated
SRR24920652 is single end
SRR24920652 is conventional basespace
SRR24920652 read1 length is 75 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR24920652_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	75
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.156	37.0	37.0	37.0	37.0	37.0
2	36.7445	37.0	37.0	37.0	37.0	37.0
3	35.8625	37.0	37.0	37.0	37.0	37.0
4	36.791	37.0	37.0	37.0	37.0	37.0
5	36.567	37.0	37.0	37.0	37.0	37.0
6	36.7385	37.0	37.0	37.0	37.0	37.0
7	36.2155	37.0	37.0	37.0	37.0	37.0
8	36.831	37.0	37.0	37.0	37.0	37.0
9	35.94	37.0	37.0	37.0	37.0	37.0
10	36.805	37.0	37.0	37.0	37.0	37.0
11	36.7375	37.0	37.0	37.0	37.0	37.0
12	36.606	37.0	37.0	37.0	37.0	37.0
13	36.3815	37.0	37.0	37.0	37.0	37.0
14	36.6825	37.0	37.0	37.0	37.0	37.0
15	36.5125	37.0	37.0	37.0	37.0	37.0
16	36.2855	37.0	37.0	37.0	37.0	37.0
17	36.6125	37.0	37.0	37.0	37.0	37.0
18	36.8125	37.0	37.0	37.0	37.0	37.0
19	36.603	37.0	37.0	37.0	37.0	37.0
20	36.6235	37.0	37.0	37.0	37.0	37.0
21	36.6025	37.0	37.0	37.0	37.0	37.0
22	36.736	37.0	37.0	37.0	37.0	37.0
23	36.5795	37.0	37.0	37.0	37.0	37.0
24	36.658	37.0	37.0	37.0	37.0	37.0
25	36.4535	37.0	37.0	37.0	37.0	37.0
26	36.439	37.0	37.0	37.0	37.0	37.0
27	36.0935	37.0	37.0	37.0	37.0	37.0
28	36.108	37.0	37.0	37.0	37.0	37.0
29	35.7425	37.0	37.0	37.0	37.0	37.0
30	35.601	37.0	37.0	37.0	37.0	37.0
31	34.5485	37.0	37.0	37.0	25.0	37.0
32	34.8875	37.0	37.0	37.0	25.0	37.0
33	35.04	37.0	37.0	37.0	25.0	37.0
34	34.844	37.0	37.0	37.0	25.0	37.0
35	34.4055	37.0	37.0	37.0	25.0	37.0
36	34.652	37.0	37.0	37.0	25.0	37.0
37	34.2175	37.0	37.0	37.0	25.0	37.0
38	33.609	37.0	37.0	37.0	25.0	37.0
39	32.977	37.0	37.0	37.0	11.0	37.0
40	33.095	37.0	37.0	37.0	11.0	37.0
41	32.407	37.0	37.0	37.0	11.0	37.0
42	32.8575	37.0	37.0	37.0	11.0	37.0
43	32.858	37.0	37.0	37.0	11.0	37.0
44	32.759	37.0	37.0	37.0	11.0	37.0
45	31.9665	37.0	25.0	37.0	11.0	37.0
46	32.601	37.0	37.0	37.0	11.0	37.0
47	32.0215	37.0	25.0	37.0	11.0	37.0
48	32.095	37.0	25.0	37.0	11.0	37.0
49	30.542	37.0	25.0	37.0	11.0	37.0
50	31.9615	37.0	25.0	37.0	11.0	37.0
51	31.6595	37.0	25.0	37.0	11.0	37.0
52	32.06	37.0	25.0	37.0	11.0	37.0
53	30.9355	37.0	25.0	37.0	11.0	37.0
54	31.4925	37.0	25.0	37.0	11.0	37.0
55	31.4085	37.0	25.0	37.0	11.0	37.0
56	31.321	37.0	25.0	37.0	11.0	37.0
57	30.786	37.0	25.0	37.0	11.0	37.0
58	31.33	37.0	25.0	37.0	11.0	37.0
59	31.2085	37.0	25.0	37.0	11.0	37.0
60	30.734	37.0	25.0	37.0	11.0	37.0
61	30.3305	37.0	25.0	37.0	11.0	37.0
62	31.321	37.0	25.0	37.0	11.0	37.0
63	31.4075	37.0	25.0	37.0	11.0	37.0
64	31.023	37.0	25.0	37.0	11.0	37.0
65	30.21	37.0	25.0	37.0	11.0	37.0
66	30.864	37.0	25.0	37.0	11.0	37.0
67	30.6215	37.0	25.0	37.0	11.0	37.0
68	30.6175	37.0	25.0	37.0	11.0	37.0
69	30.123	37.0	25.0	37.0	11.0	37.0
70	30.155	37.0	25.0	37.0	11.0	37.0
71	29.7195	37.0	25.0	37.0	11.0	37.0
72	29.757	37.0	25.0	37.0	11.0	37.0
73	28.58	37.0	25.0	37.0	11.0	37.0
74	29.342	37.0	25.0	37.0	11.0	37.0
75	29.3335	37.0	25.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
1101	52	0.0
1101	53	0.0
1101	54	0.0
1101	55	0.0
1101	56	0.0
1101	57	0.0
1101	58	0.0
1101	59	0.0
1101	60	0.0
1101	61	0.0
1101	62	0.0
1101	63	0.0
1101	64	0.0
1101	65	0.0
1101	66	0.0
1101	67	0.0
1101	68	0.0
1101	69	0.0
1101	70	0.0
1101	71	0.0
1101	72	0.0
1101	73	0.0
1101	74	0.0
1101	75	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	12.0
24	23.0
25	41.0
26	87.0
27	94.0
28	117.0
29	137.0
30	166.0
31	207.0
32	294.0
33	433.0
34	673.0
35	944.0
36	738.0
37	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	77.64999999999999	12.4	1.7999999999999998	8.15
2	68.0	11.85	6.9750000000000005	13.175
3	40.949999999999996	14.725	19.375	24.95
4	47.75	10.65	12.55	29.049999999999997
5	29.325000000000003	33.6	10.7	26.375
6	32.425	31.775	12.425	23.375
7	54.75	17.224999999999998	6.9	21.125
8	39.6	27.450000000000003	4.8500000000000005	28.1
9	59.075	14.475	6.15	20.3
10	34.4	28.175	18.3	19.125
11	52.65	11.65	10.549999999999999	25.15
12	31.15	32.125	13.125	23.599999999999998
13	36.125	20.200000000000003	30.675	13.0
14	49.975	27.525	9.525	12.975
15	37.425000000000004	37.0	7.95	17.625
16	42.725	36.4	9.275	11.600000000000001
17	28.525	48.4	10.875	12.2
18	26.724999999999998	35.325	12.049999999999999	25.900000000000002
19	20.275000000000002	42.5	5.949999999999999	31.275
20	22.875	38.324999999999996	15.15	23.65
21	23.825	43.425000000000004	9.025	23.724999999999998
22	24.9	55.375	9.049999999999999	10.674999999999999
23	27.700000000000003	59.62499999999999	7.049999999999999	5.625
24	24.7	62.625	4.475	8.200000000000001
25	17.2	69.5	4.2250000000000005	9.075
26	7.449999999999999	78.325	5.325	8.9
27	10.525	77.525	5.7	6.25
28	7.8	79.225	5.925	7.049999999999999
29	12.25	75.925	4.175	7.6499999999999995
30	12.65	75.7	7.049999999999999	4.6
31	12.1	75.675	6.550000000000001	5.675
32	16.150000000000002	72.6	6.125	5.125
33	13.975000000000001	73.4	5.7	6.925000000000001
34	13.925	70.35	9.525	6.2
35	15.174999999999999	69.77499999999999	3.9	11.15
36	21.675	68.675	3.7249999999999996	5.925
37	20.599999999999998	67.375	4.125	7.9
38	20.325	66.85	5.525	7.3
39	18.725	64.275	7.575	9.425
40	24.474999999999998	54.25	8.475000000000001	12.8
41	26.05	51.1	6.7	16.150000000000002
42	27.6	50.2	7.475	14.725
43	23.849999999999998	48.275	8.6	19.275000000000002
44	23.7	52.349999999999994	5.75	18.2
45	29.225	45.725	7.124999999999999	17.925
46	30.475	46.550000000000004	6.800000000000001	16.175
47	25.374999999999996	44.35	10.825	19.45
48	28.4	39.7	10.174999999999999	21.725
49	24.025	39.125	13.450000000000001	23.400000000000002
50	22.75	41.675000000000004	10.25	25.324999999999996
51	22.275	38.675	11.600000000000001	27.450000000000003
52	21.75	39.925	9.775	28.549999999999997
53	21.25	36.95	12.75	29.049999999999997
54	19.275000000000002	33.175	17.1	30.45
55	17.2	28.299999999999997	19.675	34.825
56	20.325	23.799999999999997	20.625	35.25
57	23.325000000000003	27.500000000000004	19.900000000000002	29.275000000000002
58	21.875	29.725	19.025	29.375
59	17.175	35.5	19.125	28.199999999999996
60	17.599999999999998	33.15	20.724999999999998	28.525
61	16.7	30.85	21.425	31.025000000000002
62	13.25	31.825	20.7	34.225
63	11.799999999999999	33.975	17.75	36.475
64	12.950000000000001	31.5	19.525000000000002	36.025
65	15.55	32.175	23.35	28.925
66	15.9	31.025000000000002	20.200000000000003	32.875
67	15.8	30.55	20.974999999999998	32.675
68	17.1	28.125	20.5	34.275
69	20.825	25.825	20.849999999999998	32.5
70	13.925	25.3	24.325	36.449999999999996
71	15.325	29.5	23.150000000000002	32.025
72	13.65	33.7	23.150000000000002	29.5
73	16.25	30.95	24.725	28.075
74	18.375	26.674999999999997	28.625	26.325
75	21.55	25.874999999999996	21.425	31.15
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	3.0
19	3.5
20	2.0
21	1.5
22	3.0
23	3.5
24	2.0
25	3.5
26	10.5
27	11.0
28	6.0
29	12.0
30	19.5
31	20.0
32	19.0
33	22.5
34	30.0
35	37.5
36	41.0
37	47.0
38	88.0
39	203.5
40	284.0
41	384.0
42	401.5
43	314.5
44	310.0
45	303.0
46	291.0
47	278.0
48	270.0
49	266.0
50	256.5
51	298.5
52	346.0
53	294.0
54	163.0
55	74.0
56	64.0
57	66.5
58	51.0
59	21.5
60	10.0
61	6.5
62	4.5
63	3.5
64	1.0
65	1.0
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6853448275862	61.724999999999994
2	6.070402298850574	8.450000000000001
3	1.329022988505747	2.775
4	1.0057471264367817	2.8000000000000003
5	0.6824712643678161	2.375
6	0.3951149425287357	1.6500000000000001
7	0.4669540229885058	2.275
8	0.10775862068965517	0.6
9	0.21551724137931033	1.35
>10	0.9698275862068966	12.45
>50	0.07183908045977011	3.55
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG	78	1.95	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATC	64	1.6	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC	36	0.8999999999999999	TruSeq Adapter, Index 3 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	32	0.8	TruSeq Adapter, Index 7 (97% over 35bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	32	0.8	TruSeq Adapter, Index 3 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	31	0.775	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	30	0.75	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTC	25	0.625	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC	25	0.625	TruSeq Adapter, Index 3 (97% over 36bp)
ACGGTGAAGAGACATGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCA	23	0.575	TruSeq Adapter, Index 3 (97% over 36bp)
GAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC	23	0.575	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCCCGGGTGGAGCCGCCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC	22	0.5499999999999999	TruSeq Adapter, Index 3 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTGAAGAGACATGAGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC	19	0.475	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGC	16	0.4	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG	15	0.375	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	14	0.35000000000000003	TruSeq Adapter, Index 3 (97% over 36bp)
GAGCGTACGCGTTAGGACCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	14	0.35000000000000003	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	14	0.35000000000000003	TruSeq Adapter, Index 3 (97% over 36bp)
GGCCGCGGGTGCGCGTCTCTCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	13	0.325	TruSeq Adapter, Index 7 (97% over 35bp)
ACGGTGAAGAGACATGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATC	13	0.325	TruSeq Adapter, Index 3 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	11	0.27499999999999997	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGTGTAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	10	0.25	TruSeq Adapter, Index 3 (97% over 36bp)
ACGGTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	10	0.25	TruSeq Adapter, Index 3 (97% over 36bp)
GAGCGTACGCGTTAGGACCCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	10	0.25	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT	10	0.25	TruSeq Adapter, Index 3 (97% over 36bp)
GGCCGCGGGTGCGCGTCTCTCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	10	0.25	TruSeq Adapter, Index 3 (97% over 36bp)
CCCCCGCCGGGCGTCGGGACCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	10	0.25	TruSeq Adapter, Index 7 (97% over 35bp)
GGTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	9	0.22499999999999998	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCTGGGGCGCGAAGCGGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG	9	0.22499999999999998	TruSeq Adapter, Index 3 (97% over 36bp)
ACGGTGAAGAGACATGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT	9	0.22499999999999998	TruSeq Adapter, Index 3 (97% over 36bp)
GGCCGCGGGTGCGCGTCTCTCGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	9	0.22499999999999998	TruSeq Adapter, Index 3 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	9	0.22499999999999998	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCCCGGGTGGAGCCGCCGCAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	9	0.22499999999999998	TruSeq Adapter, Index 3 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	8	0.2	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCCCGGGTGGAGCCGCCGCAGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	8	0.2	TruSeq Adapter, Index 7 (97% over 35bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAGCCCGGCGGGTGCCGGCGCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCCCGGGTGGAGCCGCCGCAGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	7	0.17500000000000002	TruSeq Adapter, Index 7 (97% over 35bp)
GGTGAAGAGACATGAGAGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAGAAGCCGGCGGGAGCCCCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GTGAAGAGACATGAGAGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTGAAGAGACATGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCA	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCCCGGGTGGAGCCGCCGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GTGAAGAGACATGAGAGGTGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCGCGGGTGCGCGTCTCTCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GCGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCGGCGGGTCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCG	6	0.15	TruSeq Adapter, Index 3 (97% over 36bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGAAGAGACATAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCG	6	0.15	TruSeq Adapter, Index 3 (97% over 36bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	6	0.15	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCCCGGGTGGAGCCGCCGCAGGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	6	0.15	TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGCCCGAGGTGGGATCCCGAGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	6	0.15	TruSeq Adapter, Index 7 (97% over 35bp)
CCCCCGCCGGGCGTCGGGACCGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	6	0.15	TruSeq Adapter, Index 3 (97% over 36bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAAC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGCGGGTGCGCGTCTCTCGGGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGGCCCGGGTGGAGCCGCCGCAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCG	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGTGAAGAGACATGAGAGGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
AGCTGGGGATTGTGGGTTCGTGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	5	0.125	TruSeq Adapter, Index 7 (97% over 35bp)
GAGAAGCCGGCGGGAGCCCCGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCCCGGGTGGAGCCGCCGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGGTTCCGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCG	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTGAAGAGACATGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAT	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGGGAGCCCGGCGGGTGCCGGCGCGGGTAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGTCGGCGCGCGGCGCGGGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGAGAAGCCGGCGGGAGCCCCGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGAAGAGACATGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCG	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGAAGAGACATGAGAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTC	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGGGGATTGTGGGTTCGGGTCCCATCTGGGTCGCAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.025	0.0	0.0	0.0	0.0
16	0.05	0.0	0.0	0.0	0.0
17	0.1	0.0	0.0	0.0	0.0
18	0.175	0.0	0.0	0.0	0.0
19	0.25	0.0	0.0	0.0	0.0
20	0.4	0.0	0.0	0.0	0.0
21	0.6	0.0	0.0	0.0	0.0
22	1.0	0.0	0.0	0.0	0.0
23	1.425	0.0	0.0	0.0	0.0
24	2.275	0.0	0.0	0.0	0.0
25	3.45	0.0	0.0	0.0	0.0
26	4.575	0.0	0.0	0.0	0.0
27	6.25	0.0	0.0	0.0	0.0
28	8.95	0.0	0.0	0.0	0.0
29	12.675	0.0	0.0	0.0	0.0
30	15.75	0.0	0.0	0.0	0.0
31	20.575	0.0	0.0	0.0	0.0
32	22.975	0.0	0.0	0.0	0.0
33	25.35	0.0	0.0	0.0	0.0
34	28.0	0.0	0.0	0.0	0.0
35	32.35	0.0	0.0	0.0	0.0
36	38.3	0.0	0.0	0.0	0.0
37	45.325	0.0	0.0	0.0	0.0
38	49.9	0.0	0.0	0.0	0.0
39	55.2	0.0	0.0	0.0	0.0
40	60.75	0.0	0.0	0.0	0.0
41	63.8	0.0	0.0	0.0	0.0
42	66.4	0.0	0.0	0.0	0.0
43	68.275	0.0	0.0	0.0	0.0
44	70.975	0.0	0.0	0.0	0.0
45	72.95	0.0	0.0	0.0	0.0
46	74.425	0.0	0.0	0.0	0.0
47	75.875	0.0	0.0	0.0	0.0
48	76.825	0.0	0.0	0.0	0.0
49	77.575	0.0	0.0	0.0	0.0
50	78.75	0.0	0.0	0.0	0.0
51	80.275	0.0	0.0	0.0	0.0
52	81.825	0.0	0.0	0.0	0.0
53	82.95	0.0	0.0	0.0	0.0
54	83.35	0.0	0.0	0.0	0.0
55	83.725	0.0	0.0	0.0	0.0
56	83.875	0.0	0.0	0.0	0.0
57	83.875	0.0	0.0	0.0	0.0
58	83.925	0.0	0.0	0.0	0.0
59	83.925	0.0	0.0	0.0	0.0
60	83.925	0.0	0.0	0.0	0.0
61	83.925	0.0	0.0	0.0	0.0
62	83.95	0.0	0.0	0.0	0.0
63	83.975	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGAAG	30	8.704228E-8	69.00001	1
ACGCCTG	15	0.0021872367	69.00001	7
CGCCTGT	15	0.0021872367	69.00001	8
TGTCTGA	15	0.0021872367	69.00001	12
CCCGGGT	15	0.0021872367	69.00001	4
CCTGTCT	15	0.0021872367	69.00001	10
CCGCCGC	15	0.0021872367	69.00001	15
GAGCCGC	15	0.0021872367	69.00001	12
TGGAGCC	15	0.0021872367	69.00001	10
GCTACGC	15	0.0021872367	69.00001	4
CGGGTGG	15	0.0021872367	69.00001	6
TACGCCT	15	0.0021872367	69.00001	6
CTACGCC	15	0.0021872367	69.00001	5
GGCTACG	15	0.0021872367	69.00001	3
GGGGCTA	15	0.0021872367	69.00001	1
CCGGGTG	15	0.0021872367	69.00001	5
GGTGGAG	15	0.0021872367	69.00001	8
GCCGCAG	15	0.0021872367	69.00001	17
CTGTCTG	15	0.0021872367	69.00001	11
GCCCGGG	15	0.0021872367	69.00001	3
>>END_MODULE
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
Read 1860011 spots for SRR24920652.sra
Written 1860011 spots for SRR24920652.sra
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
Read 1860005 spots for SRR24920652.sra
Written 1860005 spots for SRR24920652.sra
SRR ids: ['SRR24920652.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_arebsqop
SRR24920652.sra spots: 14880046
blocks: [[1, 1860005], [1860006, 3720010], [3720011, 5580015], [5580016, 7440020], [7440021, 9300025], [9300026, 11160030], [11160031, 13020035], [13020036, 14880046]]
SRR24920652 file size 3265544
SRR24920652 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR24920652 SRR24920652_1.fastq
Input file:	SRR24920652_1.fastq
trimmed:	SRR24920652-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Wed Jul  1 12:56:00 2026 >> started

Wed Jul  1 12:56:08 2026 >> done (7.940s)
14880046 reads processed; of these:
   24100 ( 0.16%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
14855946 (99.84%) reads available; of these:
11585662 (77.99%) trimmed reads available after processing
 3270284 (22.01%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   11519	  0.08%
 19	   17517	  0.12%
 20	   25516	  0.17%
 21	   39023	  0.26%
 22	   59477	  0.40%
 23	   87921	  0.59%
 24	  152663	  1.03%
 25	  267107	  1.80%
 26	  335035	  2.26%
 27	  465652	  3.13%
 28	  670587	  4.51%
 29	  584831	  3.94%
 30	  757252	  5.10%
 31	  390970	  2.63%
 32	  363133	  2.44%
 33	  414181	  2.79%
 34	  655846	  4.41%
 35	  944596	  6.36%
 36	 1004114	  6.76%
 37	  715396	  4.82%
 38	  936588	  6.30%
 39	  918018	  6.18%
 40	  530921	  3.57%
 41	  497391	  3.35%
 42	  350064	  2.36%
 43	  390326	  2.63%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       0	  0.00%
 69	       0	  0.00%
 70	       0	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       0	  0.00%
 74	      18	  0.00%
 75	 3270284	 22.01%
14855946 reads passed initial QC


criterion=sequence-density
sequence-density=2.44
sequence-density-rank=1
fanout-score=2.97
fanout-score-rank=7
prefix-density=2.44
prefix-fanout=3.0
sequence=GTCCCATCTGGGTCGCCAAAAAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=31
fanout-score=58.67
fanout-score-rank=1
prefix-density=0.20
prefix-fanout=2.9
sequence=CGGGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTCCCATCTGGGTCGCCAAAAAAAAA -o SRR24920652 -
Input file:	STDIN
trimmed:	SRR24920652-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCCCATCTGGGTCGCCAAAAAAAAA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Wed Jul  1 12:56:22 2026 >> started

Wed Jul  1 12:56:26 2026 >> done (3.473s)
4951982 reads processed; of these:
    557 ( 0.01%) short reads filtered out after trimming by size control
    177 ( 0.00%) empty reads filtered out after trimming by size control
4951248 (99.99%) reads available; of these:
  91072 ( 1.84%) trimmed reads available after processing
4860176 (98.16%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   3906	  0.08%
 19	  11662	  0.24%
 20	  10188	  0.21%
 21	  73072	  1.48%
 22	  23753	  0.48%
 23	  30275	  0.61%
 24	  50990	  1.03%
 25	  89050	  1.80%
 26	 111717	  2.26%
 27	 155465	  3.14%
 28	 223199	  4.51%
 29	 194916	  3.94%
 30	 252115	  5.09%
 31	 130311	  2.63%
 32	 121468	  2.45%
 33	 137568	  2.78%
 34	 217979	  4.40%
 35	 315747	  6.38%
 36	 334348	  6.75%
 37	 238432	  4.82%
 38	 313150	  6.32%
 39	 305492	  6.17%
 40	 176476	  3.56%
 41	 165787	  3.35%
 42	 116950	  2.36%
 43	 130262	  2.63%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      4	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      2	  0.00%
 66	      4	  0.00%
 67	    143	  0.00%
 68	    253	  0.01%
 69	    275	  0.01%
 70	    575	  0.01%
 71	    588	  0.01%
 72	  16690	  0.34%
 73	      0	  0.00%
 74	      2	  0.00%
 75	 998434	 20.17%


criterion=sequence-density
sequence-density=1.71
sequence-density-rank=1
fanout-score=12.86
fanout-score-rank=2
prefix-density=0.01
prefix-fanout=12.9
sequence=AAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=31
fanout-score=49.05
fanout-score-rank=1
prefix-density=0.19
prefix-fanout=2.9
sequence=CGGGCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCTC
                                 Started job on |	Jul 01 12:56:58
                             Started mapping on |	Jul 01 12:56:58
                                    Finished on |	Jul 01 12:58:00
       Mapping speed, Million of reads per hour |	862.56

                          Number of input reads |	14855212
                      Average input read length |	38
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3609152
                        Uniquely mapped reads % |	24.30%
                          Average mapped length |	24.44
                       Number of splices: Total |	263567
            Number of splices: Annotated (sjdb) |	1007
                       Number of splices: GT/AG |	205676
                       Number of splices: GC/AG |	11297
                       Number of splices: AT/AC |	203
               Number of splices: Non-canonical |	46391
                      Mismatch rate per base, % |	1.08%
                         Deletion rate per base |	0.00%
                        Deletion average length |	2.29
                        Insertion rate per base |	0.00%
                       Insertion average length |	2.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4785683
             % of reads mapped to multiple loci |	32.22%
        Number of reads mapped to too many loci |	549287
             % of reads mapped to too many loci |	3.70%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	38.24%
                     % of reads unmapped: other |	1.55%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6460377	6460377	6460377
N_multimapping	4785683	4785683	4785683
N_noFeature	3384468	3493101	3494070
N_ambiguous	8045	1520	2695
UnstrandedReadsAssigned:216639 PositiveStrandReadsAssigned:114531 NegativeStrandReadsAssigned:112387
Dataset is classified unstranded
MeadianReadLen=32 20thPercentileLength=26 echo kmer=21
SRR24920652 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=21

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 21
[index] number of targets: 109,282
[index] number of k-mers: 93,655,514
[index] number of equivalence classes: 565,482
[quant] running in single-end mode
[quant] will process file 1: SRR24920652-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 14,855,212 reads, 2,165,171 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,233 rounds

 109283 SRR24920652.ke.tsv
  52636 SRR24920652.se.tsv
 161919 total
==> SRR24920652.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR24920652.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR24920652 completed mapping pipeline successfully
