Starting /dee2/code/volunteer_pipeline.sh SRR25183864
    current disk space = 1256630340
    free memory = 201004012 
SRR25183864 SRAfilesize
fe42ffb8da93fed0dabf42b254d19a3c  SRR25183864.sra
SRR25183864.sra file validated
SRR25183864 is paired end
SRR25183864 is conventional basespace
SRR25183864 read1 length is 45-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25183864_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	45-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.33175	37.0	37.0	37.0	37.0	37.0
2	36.3265	37.0	37.0	37.0	37.0	37.0
3	36.3555	37.0	37.0	37.0	37.0	37.0
4	36.3885	37.0	37.0	37.0	37.0	37.0
5	36.382	37.0	37.0	37.0	37.0	37.0
6	36.398	37.0	37.0	37.0	37.0	37.0
7	36.333	37.0	37.0	37.0	37.0	37.0
8	36.3395	37.0	37.0	37.0	37.0	37.0
9	36.386	37.0	37.0	37.0	37.0	37.0
10-14	36.3837	37.0	37.0	37.0	37.0	37.0
15-19	36.2746	37.0	37.0	37.0	37.0	37.0
20-24	36.3065	37.0	37.0	37.0	37.0	37.0
25-29	36.2139	37.0	37.0	37.0	37.0	37.0
30-34	36.1996	37.0	37.0	37.0	37.0	37.0
35-39	36.16	37.0	37.0	37.0	37.0	37.0
40-44	36.0824	37.0	37.0	37.0	37.0	37.0
45-49	36.01360375187594	37.0	37.0	37.0	37.0	37.0
50-54	36.08539713940032	37.0	37.0	37.0	37.0	37.0
55-59	36.00650685711982	37.0	37.0	37.0	37.0	37.0
60-64	35.902236839389104	37.0	37.0	37.0	37.0	37.0
65-69	35.83280887518232	37.0	37.0	37.0	37.0	37.0
70-74	35.81586076727571	37.0	37.0	37.0	37.0	37.0
75-79	35.896206151779936	37.0	37.0	37.0	37.0	37.0
80-84	35.85765316729574	37.0	37.0	37.0	37.0	37.0
85-89	35.78295758692309	37.0	37.0	37.0	37.0	37.0
90-94	35.6752924211677	37.0	37.0	37.0	37.0	37.0
95-99	35.64841872227632	37.0	37.0	37.0	37.0	37.0
100-104	35.5941135235187	37.0	37.0	37.0	37.0	37.0
105-109	35.55688562567528	37.0	37.0	37.0	37.0	37.0
110-114	35.54088333847676	37.0	37.0	37.0	37.0	37.0
115-119	35.396449242797544	37.0	37.0	37.0	37.0	37.0
120-124	35.49977409832791	37.0	37.0	37.0	37.0	37.0
125-129	35.26325440919	37.0	37.0	37.0	27.4	37.0
130-134	35.289261719971016	37.0	37.0	37.0	29.8	37.0
135-139	35.168354410881626	37.0	37.0	37.0	27.4	37.0
140-144	34.94946282144745	37.0	37.0	37.0	25.0	37.0
145-149	34.71869085190894	37.0	37.0	37.0	25.0	37.0
150	34.58127141225733	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	7.0
26	12.0
27	12.0
28	18.0
29	27.0
30	53.0
31	53.0
32	92.0
33	125.0
34	194.0
35	564.0
36	2684.0
37	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.084521130282575	8.32708177044261	15.9039759939985	37.684421105276314
2	25.1	12.775	32.85	29.275000000000002
3	26.85	18.075	24.175	30.9
4	29.849999999999998	26.275	20.775	23.1
5	28.975	29.775000000000002	22.925	18.325
6	19.900000000000002	35.55	25.374999999999996	19.175
7	14.424999999999999	22.125	42.55	20.9
8	15.525	26.1	35.025	23.35
9	16.75	24.85	34.275	24.125
10-14	20.925	30.69	26.965	21.42
15-19	20.849999999999998	26.950000000000003	28.860000000000003	23.34
20-24	20.68	27.224999999999998	29.235	22.86
25-29	20.349999999999998	26.155	29.965000000000003	23.53
30-34	20.68	27.91	28.7	22.71
35-39	21.57	28.415000000000003	28.325	21.69
40-44	21.975	27.005000000000003	28.060000000000002	22.96
45-49	20.048019207683073	28.266306522609042	28.746498599439775	22.939175670268106
50-54	21.631897543649007	26.59462704487468	29.496222922607434	22.27725248886888
55-59	20.176140912730183	28.547838270616495	28.793034427542036	22.48298638911129
60-64	22.33909341347358	28.20936639118457	27.232657150012525	22.218883045329328
65-69	21.081162475530792	27.510917030567683	28.871153942679317	22.536766551222208
70-74	20.410731363567724	27.16061811043439	27.568329390446465	24.860321135551416
75-79	21.6422435573522	28.039413845376455	28.059626073774634	22.258716523496716
80-84	21.191311867338115	28.032962002136426	27.982094714888856	22.793631415636604
85-89	21.86490229266041	28.250500076934916	27.168282299841	22.716315330563678
90-94	23.473302229134266	28.190772420943492	26.30896837739762	22.026956972524626
95-99	21.99305482479217	28.58044827949069	26.7652320319899	22.661264863727244
100-104	21.320450885668276	27.632850241545892	26.93505099302201	24.111647879763822
105-109	20.824605886892293	27.543820967919746	28.117076397310107	23.514496747877853
110-114	21.287269824981472	27.575394789350664	27.61530129411094	23.52203409155692
115-119	21.774904496032914	27.064354980899207	29.468116367910667	21.692624155157215
120-124	22.681543808304372	25.62648618986647	28.144625327723922	23.547344674105236
125-129	21.408146406557794	27.62279977123975	28.029484654000125	22.939569168202325
130-134	21.248427881114715	27.05368372277752	28.860793009862977	22.837095386244787
135-139	20.55939226519337	27.59668508287293	27.838397790055247	24.005524861878452
140-144	20.421522083153505	27.22629837433463	29.31952237088189	23.03265717162998
145-149	20.835204310731925	29.344409519533006	27.039365364466395	22.781020805268675
150	20.74609821088694	28.321279025504374	27.864484202512372	23.06813856109631
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.0
20	1.0
21	3.0
22	3.5
23	3.5
24	6.5
25	12.5
26	34.5
27	49.5
28	67.5
29	99.0
30	89.5
31	60.0
32	63.0
33	68.5
34	69.5
35	90.0
36	112.5
37	126.5
38	113.5
39	112.0
40	145.5
41	147.5
42	145.0
43	183.0
44	189.0
45	176.5
46	185.0
47	167.0
48	154.5
49	129.5
50	109.5
51	116.0
52	122.0
53	118.5
54	82.5
55	68.5
56	79.0
57	57.0
58	51.0
59	80.5
60	84.5
61	50.0
62	25.5
63	27.0
64	21.0
65	17.0
66	15.0
67	15.0
68	19.0
69	16.5
70	12.5
71	17.5
72	14.0
73	5.5
74	1.5
75	0.0
76	0.0
77	0.0
78	0.5
79	0.5
80	0.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	2.0
50-54	1.0
55-59	1.0
60-64	6.0
65-69	10.0
70-74	14.0
75-79	23.0
80-84	31.0
85-89	31.0
90-94	62.0
95-99	59.0
100-104	94.0
105-109	104.0
110-114	118.0
115-119	102.0
120-124	144.0
125-129	132.0
130-134	128.0
135-139	105.0
140-144	128.0
145-149	78.0
150-151	2627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.12993854258121	38.800000000000004
2	16.286215978928887	18.55
3	7.111501316944688	12.15
4	2.9850746268656714	6.800000000000001
5	2.019315188762072	5.75
6	1.141352063213345	3.9
7	0.7901668129938543	3.15
8	0.21949078138718175	1.0
9	0.2633889376646181	1.35
>10	1.0535557506584723	8.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	24	0.6	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	23	0.575	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	23	0.575	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	21	0.525	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	19	0.475	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	18	0.44999999999999996	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	15	0.375	No Hit
GGCAACTACAGTGATGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAA	15	0.375	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	15	0.375	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	14	0.35000000000000003	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	14	0.35000000000000003	No Hit
GTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCTAACCT	13	0.325	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	13	0.325	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	12	0.3	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	11	0.27499999999999997	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	11	0.27499999999999997	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	11	0.27499999999999997	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	10	0.25	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	10	0.25	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	10	0.25	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	10	0.25	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	10	0.25	No Hit
GCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCA	10	0.25	No Hit
GCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGAT	10	0.25	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	9	0.22499999999999998	No Hit
GCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTA	9	0.22499999999999998	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	9	0.22499999999999998	No Hit
CCAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAG	9	0.22499999999999998	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	9	0.22499999999999998	No Hit
GTTGGACTAAGGTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTG	9	0.22499999999999998	No Hit
CTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCA	8	0.2	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	8	0.2	No Hit
GTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCACTTACATCTTACC	8	0.2	No Hit
CCGATTTAATAAGTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCACT	8	0.2	No Hit
CTGGCATCACAGGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTG	8	0.2	No Hit
CTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGT	7	0.17500000000000002	No Hit
CCGGGTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTAC	7	0.17500000000000002	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	7	0.17500000000000002	No Hit
CCGGACACCCACGGCCACTTCATGTAAACCACTGACACTTTGAGAAACTA	7	0.17500000000000002	No Hit
CTCCACCTCATGGGCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTG	7	0.17500000000000002	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	7	0.17500000000000002	No Hit
CGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATAGCT	7	0.17500000000000002	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	7	0.17500000000000002	No Hit
ATCGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTA	7	0.17500000000000002	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	7	0.17500000000000002	No Hit
GCTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCAC	7	0.17500000000000002	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	7	0.17500000000000002	No Hit
TGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGCA	7	0.17500000000000002	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	7	0.17500000000000002	No Hit
CCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTC	7	0.17500000000000002	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	7	0.17500000000000002	No Hit
GCCGATTTAATAAGTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCAC	7	0.17500000000000002	No Hit
CTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTA	7	0.17500000000000002	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	6	0.15	No Hit
GCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCA	6	0.15	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	6	0.15	No Hit
GTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAGGACA	6	0.15	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	6	0.15	No Hit
CTCTTTCTCACGCTGCAGCTCCACCTCATGGGCCTCTCGAAGCAGGGCCT	6	0.15	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	6	0.15	No Hit
CCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTT	6	0.15	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	6	0.15	No Hit
CGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACCAT	6	0.15	No Hit
TGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATT	6	0.15	No Hit
GTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCT	6	0.15	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	6	0.15	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	6	0.15	No Hit
TGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTC	6	0.15	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	6	0.15	No Hit
CGCCACTATATACAAACACAGGACAACCTGAAGCTAAATGGATGCCCACC	6	0.15	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	6	0.15	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	6	0.15	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	6	0.15	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	6	0.15	No Hit
CTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCT	6	0.15	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	6	0.15	No Hit
CTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTTTCTGCACCATTTTG	6	0.15	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	6	0.15	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	6	0.15	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	5	0.125	No Hit
GCACCACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATA	5	0.125	No Hit
CCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTG	5	0.125	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	5	0.125	No Hit
TCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACCA	5	0.125	No Hit
GCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGTG	5	0.125	No Hit
CTTGGCTAGAGAGGTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCAT	5	0.125	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	5	0.125	No Hit
TTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGC	5	0.125	No Hit
GCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAG	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
CGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATT	5	0.125	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	5	0.125	No Hit
ATAGACACTTAAAAGGATCATATATTTCCCATGAGGGTAACATCATTTAG	5	0.125	No Hit
CAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGA	5	0.125	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	5	0.125	No Hit
CTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATA	5	0.125	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	5	0.125	No Hit
CTCAAAATGTCAAAATAAAATATATGACAGAGAGGCAATATTAGTTTTCT	5	0.125	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	5	0.125	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	5	0.125	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	5	0.125	No Hit
GGCGGGGCTCCGACATCTGCACAGCTACAACTGAATCTCACGGTAGGCCC	5	0.125	No Hit
CCTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATAC	5	0.125	No Hit
GTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGA	5	0.125	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	5	0.125	No Hit
GCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTC	5	0.125	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
GCTATGTACAACTTTGATACAGTTTCAGGGCGCTCCGGACACCCACGGCC	5	0.125	No Hit
CCTGAATACTATGGGCACAGGCTGAAGGCCAGCGGACATATAGTAAGTCT	5	0.125	No Hit
GGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCT	5	0.125	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	5	0.125	No Hit
GGGGCATCAAGGAAGTTGAATGCAGGGCCTTCCATAATTCTGGCACAAAT	5	0.125	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	5	0.125	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	5	0.125	No Hit
CCGCATTCTTTTTCTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACC	5	0.125	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	5	0.125	No Hit
CTCAAAAGCATTATCCCCATTATTAGGGATAATGTAATACCATCTGATTC	5	0.125	No Hit
GGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCT	5	0.125	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	5	0.125	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	5	0.125	No Hit
GCTCCGGACACCCACGGCCACTTCATGTAAACCACTGACACTTTGAGAAA	5	0.125	No Hit
GCTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCTGTATCTCAA	5	0.125	No Hit
AATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTC	5	0.125	No Hit
CTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25183864 read2 length is 45-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25183864_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	45-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1905	37.0	37.0	37.0	37.0	37.0
2	36.347	37.0	37.0	37.0	37.0	37.0
3	36.33375	37.0	37.0	37.0	37.0	37.0
4	36.327	37.0	37.0	37.0	37.0	37.0
5	36.249	37.0	37.0	37.0	37.0	37.0
6	36.357	37.0	37.0	37.0	37.0	37.0
7	36.2895	37.0	37.0	37.0	37.0	37.0
8	36.308	37.0	37.0	37.0	37.0	37.0
9	36.4515	37.0	37.0	37.0	37.0	37.0
10-14	36.336	37.0	37.0	37.0	37.0	37.0
15-19	36.3116	37.0	37.0	37.0	37.0	37.0
20-24	36.3326	37.0	37.0	37.0	37.0	37.0
25-29	36.2789	37.0	37.0	37.0	37.0	37.0
30-34	36.26565	37.0	37.0	37.0	37.0	37.0
35-39	36.240300000000005	37.0	37.0	37.0	37.0	37.0
40-44	36.183299999999996	37.0	37.0	37.0	37.0	37.0
45-49	36.23559184592297	37.0	37.0	37.0	37.0	37.0
50-54	36.19826132731114	37.0	37.0	37.0	37.0	37.0
55-59	36.08036367616052	37.0	37.0	37.0	37.0	37.0
60-64	36.07577252714593	37.0	37.0	37.0	37.0	37.0
65-69	36.063937952974484	37.0	37.0	37.0	37.0	37.0
70-74	36.00957089934723	37.0	37.0	37.0	37.0	37.0
75-79	35.884670510203904	37.0	37.0	37.0	37.0	37.0
80-84	35.82416472232335	37.0	37.0	37.0	37.0	37.0
85-89	35.76878188459516	37.0	37.0	37.0	37.0	37.0
90-94	35.734519801596356	37.0	37.0	37.0	37.0	37.0
95-99	35.68174892525015	37.0	37.0	37.0	37.0	37.0
100-104	35.5562004579795	37.0	37.0	37.0	37.0	37.0
105-109	35.53881705745469	37.0	37.0	37.0	37.0	37.0
110-114	35.40015599616176	37.0	37.0	37.0	34.6	37.0
115-119	35.292714216504635	37.0	37.0	37.0	32.2	37.0
120-124	35.19757844602466	37.0	37.0	37.0	27.4	37.0
125-129	35.0786937451021	37.0	37.0	37.0	27.4	37.0
130-134	34.981044228107876	37.0	37.0	37.0	25.0	37.0
135-139	34.812322171280115	37.0	37.0	37.0	25.0	37.0
140-144	34.83508537995573	37.0	37.0	37.0	25.0	37.0
145-149	34.48300876183009	37.0	37.0	37.0	25.0	37.0
150	34.73501336387935	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	4.0
26	5.0
27	7.0
28	14.0
29	32.0
30	29.0
31	45.0
32	70.0
33	147.0
34	262.0
35	689.0
36	2401.0
37	294.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.625	18.725	14.799999999999999	30.85
2	24.175	29.349999999999998	28.95	17.525
3	25.98149537384346	31.45786446611653	21.655413853463365	20.905226306576644
4	28.925	34.425	19.5	17.150000000000002
5	27.625	35.3	20.0	17.075000000000003
6	23.125	35.4	22.225	19.25
7	22.1	20.575	34.75	22.575
8	23.175	23.175	26.5	27.150000000000002
9	24.275	23.625	26.900000000000002	25.2
10-14	25.45	27.589999999999996	24.51	22.45
15-19	24.779999999999998	25.685000000000002	26.305	23.23
20-24	23.705000000000002	27.96	25.145	23.189999999999998
25-29	23.965	26.31	26.36	23.365
30-34	23.58617930896545	26.066303315165758	27.04135206760338	23.306165308265413
35-39	24.01	26.68	26.345000000000002	22.965
40-44	24.265	26.889999999999997	25.765	23.080000000000002
45-49	23.659463785514205	26.265506202480992	26.87074829931973	23.204281712685074
50-54	23.878132973135223	26.85477012356796	26.959827905347943	22.307268997948874
55-59	22.938350680544435	26.85648518815052	27.577061649319457	22.628102481985586
60-64	24.157275231655397	26.786877034810917	26.37114951164538	22.684698221888304
65-69	23.445264267429604	26.677709180344326	27.114390403051747	22.76263614917432
70-74	23.400614083656315	26.023053304474757	27.75960134897065	22.816731262898273
75-79	23.865588681152097	27.82213239009601	26.851945426983328	21.46033350176857
80-84	24.102146708719097	26.304812290161767	27.36799267473802	22.225048326381117
85-89	22.99446040213377	27.149158801805502	27.8621255642183	21.99425523184243
90-94	22.887506480041473	27.807153965785382	26.951788491446344	22.3535510627268
95-99	23.525388055774794	28.28729281767956	27.834780320968168	20.35253880557748
100-104	23.477000697761795	29.51532392249477	26.525683001449195	20.48199237829424
105-109	23.14008596935964	29.21304970792461	26.683566626253718	20.96329769646203
110-114	22.48760189249273	29.396340420680612	26.73430998118908	21.381747705637576
115-119	22.979723773141345	28.886276814575375	27.18777549221275	20.946223920070526
120-124	22.78658536585366	29.073170731707314	27.59756097560976	20.54268292682927
125-129	23.481575603557815	29.02160101651842	26.626429479034307	20.870393900889454
130-134	22.92011383943345	29.82990270699583	26.67946257197697	20.57052088159375
135-139	23.098163744304845	29.635510147728844	26.321966036172856	20.944360071793454
140-144	22.23821518531846	30.65131342209428	27.448722562072685	19.661748830514576
145-149	22.607520828642198	30.556181040306235	27.208586654657356	19.627711476394204
150	22.107674684994272	31.767850324551354	28.10232913325697	18.0221458571974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.5
20	2.5
21	2.0
22	5.5
23	8.0
24	11.0
25	17.5
26	35.0
27	62.5
28	81.5
29	92.0
30	81.5
31	55.0
32	51.5
33	57.0
34	80.0
35	96.0
36	86.5
37	96.0
38	86.0
39	95.0
40	137.0
41	153.5
42	159.0
43	182.5
44	186.0
45	148.5
46	128.0
47	116.5
48	118.0
49	99.5
50	76.0
51	89.5
52	110.5
53	113.0
54	99.5
55	78.0
56	56.0
57	52.5
58	57.0
59	73.5
60	73.5
61	38.0
62	20.5
63	23.0
64	27.5
65	44.5
66	42.5
67	44.5
68	46.5
69	39.5
70	61.5
71	91.0
72	85.0
73	42.5
74	10.0
75	2.5
76	2.0
77	1.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.005
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.005086728724757109
85-89	0.005128994204236549
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	2.0
50-54	1.0
55-59	1.0
60-64	6.0
65-69	10.0
70-74	14.0
75-79	23.0
80-84	31.0
85-89	31.0
90-94	62.0
95-99	59.0
100-104	93.0
105-109	104.0
110-114	119.0
115-119	102.0
120-124	144.0
125-129	131.0
130-134	128.0
135-139	104.0
140-144	137.0
145-149	79.0
150-151	2619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.02112676056338	37.5
2	18.133802816901408	20.599999999999998
3	7.174295774647888	12.225
4	3.389084507042254	7.7
5	2.024647887323944	5.75
6	0.9242957746478873	3.15
7	0.6602112676056338	2.625
8	0.5721830985915494	2.6
9	0.17605633802816903	0.8999999999999999
>10	0.9242957746478873	6.950000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	25	0.625	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	17	0.42500000000000004	No Hit
GAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGG	16	0.4	No Hit
CAAGAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCC	15	0.375	No Hit
GGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAG	15	0.375	No Hit
GTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCA	15	0.375	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	14	0.35000000000000003	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	14	0.35000000000000003	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	14	0.35000000000000003	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	14	0.35000000000000003	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	13	0.325	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	12	0.3	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	12	0.3	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	11	0.27499999999999997	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	11	0.27499999999999997	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	10	0.25	No Hit
GGATCATCGACACCCCCATCTCAGAGATGGGCTTTGCTGGAATTGCTGTT	10	0.25	No Hit
GGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGA	10	0.25	No Hit
CTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGC	10	0.25	No Hit
CCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCA	10	0.25	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	10	0.25	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	9	0.22499999999999998	No Hit
GCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGA	9	0.22499999999999998	No Hit
CAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTG	9	0.22499999999999998	No Hit
CAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATT	9	0.22499999999999998	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	8	0.2	No Hit
CTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCG	8	0.2	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	8	0.2	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	8	0.2	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	8	0.2	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	8	0.2	No Hit
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	8	0.2	No Hit
GCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGG	8	0.2	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	8	0.2	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	8	0.2	No Hit
CGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGCTGG	8	0.2	No Hit
GCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGT	8	0.2	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	8	0.2	No Hit
GTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGG	7	0.17500000000000002	No Hit
GTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCG	7	0.17500000000000002	No Hit
GGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATA	7	0.17500000000000002	No Hit
CTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCC	7	0.17500000000000002	No Hit
AAGACATCCTCGCACATCAGGTCCCTAATGCCAAGAAGCTCAGGCGAAAG	7	0.17500000000000002	No Hit
GCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCC	7	0.17500000000000002	No Hit
CAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGA	7	0.17500000000000002	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	7	0.17500000000000002	No Hit
TGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCG	7	0.17500000000000002	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	7	0.17500000000000002	No Hit
TATTAATCAAGGTATGGATGAAGAACTAGAAAGAGATGAAAAAGTTTTTC	7	0.17500000000000002	No Hit
GCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAG	7	0.17500000000000002	No Hit
AAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGT	7	0.17500000000000002	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	7	0.17500000000000002	No Hit
AAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGC	7	0.17500000000000002	No Hit
CTCAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTC	6	0.15	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	6	0.15	No Hit
GTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTG	6	0.15	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	6	0.15	No Hit
GTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACT	6	0.15	No Hit
GAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCT	6	0.15	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	6	0.15	No Hit
GGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGA	6	0.15	No Hit
TAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGA	6	0.15	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	6	0.15	No Hit
TGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCC	6	0.15	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	6	0.15	No Hit
TGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGG	6	0.15	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	6	0.15	No Hit
ACTTACTATATGTCCGCTGGCCTTCAGCCTGTGCCCATAGTATTCAGGGG	6	0.15	No Hit
CGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCC	6	0.15	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	6	0.15	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	6	0.15	No Hit
GGAAGAGGAGGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAG	6	0.15	No Hit
GTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCC	6	0.15	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	6	0.15	No Hit
CGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGAT	5	0.125	No Hit
CGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAATCAAGGTATGGATG	5	0.125	No Hit
AAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAA	5	0.125	No Hit
TAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGCTGTGAT	5	0.125	No Hit
GTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGA	5	0.125	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	5	0.125	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	5	0.125	No Hit
GTAATATCTTGGCATATTTAGAAATACTTGTGACTGGCTTCAAAATATAA	5	0.125	No Hit
ATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGC	5	0.125	No Hit
CCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAA	5	0.125	No Hit
TTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGC	5	0.125	No Hit
AGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAACAAACAAACA	5	0.125	No Hit
CGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTG	5	0.125	No Hit
CAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAG	5	0.125	No Hit
GAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGC	5	0.125	No Hit
CTTCGGGCTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATAT	5	0.125	No Hit
GTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCT	5	0.125	No Hit
GCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCGCTGTGG	5	0.125	No Hit
GCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAG	5	0.125	No Hit
GGGCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCG	5	0.125	No Hit
GCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGG	5	0.125	No Hit
GAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTT	5	0.125	No Hit
GGCATGATGCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTT	5	0.125	No Hit
GTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACCCTCATG	5	0.125	No Hit
CACAGTAGTAGAGTATAAACCTCTTCCCATGACTGACCATAGAACTTCTA	5	0.125	No Hit
GAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGC	5	0.125	No Hit
GCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGAT	5	0.125	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	5	0.125	No Hit
TAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGC	5	0.125	No Hit
AAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCC	5	0.125	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	5	0.125	No Hit
CAAAGACATCCTCGCACATCAGGTCCCTAATGCCAAGAAGCTCAGGCGAA	5	0.125	No Hit
GAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGC	5	0.125	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	5	0.125	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	5	0.125	No Hit
CAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCA	5	0.125	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	5	0.125	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	5	0.125	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	5	0.125	No Hit
AGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAAT	5	0.125	No Hit
GTATTTTTCTGTTTGTATTCACATAGATGATACTATAATAGCTAAGAATC	5	0.125	No Hit
GTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTG	5	0.125	No Hit
CAGAGATGGGCTTTGCTGGAATTGCTGTTGGTGCAGCTATGGCTGGGTTG	5	0.125	No Hit
AAGAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCA	5	0.125	No Hit
GCCTTTTGGAGGGGGCCGTAGCTCAGGGCGTGCACTGTGAGGCTGGACCT	5	0.125	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0125	0.0	0.0	0.0	0.0
104-105	0.025	0.0	0.0	0.0	0.0
106-107	0.025	0.0	0.0	0.0	0.0
108-109	0.025	0.0	0.0	0.0	0.0
110-111	0.025	0.0	0.0	0.0	0.0
112-113	0.025	0.0	0.0	0.0	0.0
114-115	0.025	0.0	0.0	0.0	0.0
116-117	0.025	0.0	0.0	0.0	0.0
118-119	0.025	0.0	0.0	0.0	0.0
120-121	0.025	0.0	0.0	0.0	0.0
122-123	0.025	0.0	0.0	0.0	0.0
124-125	0.025	0.0	0.0	0.0	0.0
126-127	0.025	0.0	0.0	0.0	0.0
128-129	0.025	0.0	0.0	0.0	0.0
130-131	0.025	0.0	0.0	0.0	0.0
132-133	0.025	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
Read 3955172 spots for SRR25183864.sra
Written 3955172 spots for SRR25183864.sra
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
Read 3955166 spots for SRR25183864.sra
Written 3955166 spots for SRR25183864.sra
SRR ids: ['SRR25183864.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ym7xe0v5
SRR25183864.sra spots: 31641334
blocks: [[1, 3955166], [3955167, 7910332], [7910333, 11865498], [11865499, 15820664], [15820665, 19775830], [19775831, 23730996], [23730997, 27686162], [27686163, 31641334]]
SRR25183864 file size 10089320
SRR25183864 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25183864 SRR25183864_1.fastq SRR25183864_2.fastq
Input file:	SRR25183864_1.fastq
Paired file:	SRR25183864_2.fastq
trimmed:	SRR25183864-trimmed-pair1.fastq, SRR25183864-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun Jun 21 07:22:58 2026 >> started

Sun Jun 21 07:23:29 2026 >> done (31.189s)
31641334 read pairs processed; of these:
      21 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
31641312 (100.00%) read pairs available; of these:
  200055 ( 0.63%) trimmed read pairs available after processing
31441257 (99.37%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      10	  0.00%
 19	      12	  0.00%
 20	      15	  0.00%
 21	      19	  0.00%
 22	      32	  0.00%
 23	      26	  0.00%
 24	      17	  0.00%
 25	      66	  0.00%
 26	      22	  0.00%
 27	      35	  0.00%
 28	     194	  0.00%
 29	      90	  0.00%
 30	     156	  0.00%
 31	      32	  0.00%
 32	      46	  0.00%
 33	       7	  0.00%
 34	     215	  0.00%
 35	    1526	  0.00%
 36	     913	  0.00%
 37	     883	  0.00%
 38	     945	  0.00%
 39	    5236	  0.02%
 40	     991	  0.00%
 41	     879	  0.00%
 42	     940	  0.00%
 43	    1059	  0.00%
 44	    1310	  0.00%
 45	    1045	  0.00%
 46	    1533	  0.00%
 47	    1268	  0.00%
 48	    1647	  0.01%
 49	    1569	  0.00%
 50	    2355	  0.01%
 51	    2334	  0.01%
 52	    2439	  0.01%
 53	    2498	  0.01%
 54	    2688	  0.01%
 55	    3888	  0.01%
 56	    3222	  0.01%
 57	    3604	  0.01%
 58	    4208	  0.01%
 59	    4487	  0.01%
 60	    4938	  0.02%
 61	    5577	  0.02%
 62	    5800	  0.02%
 63	    6328	  0.02%
 64	    6798	  0.02%
 65	    7668	  0.02%
 66	    8277	  0.03%
 67	    9228	  0.03%
 68	   10283	  0.03%
 69	   11643	  0.04%
 70	   12811	  0.04%
 71	   14103	  0.04%
 72	   15861	  0.05%
 73	   17099	  0.05%
 74	   18612	  0.06%
 75	   20674	  0.07%
 76	   22756	  0.07%
 77	   24518	  0.08%
 78	   26823	  0.08%
 79	   29349	  0.09%
 80	   32442	  0.10%
 81	   35185	  0.11%
 82	   38443	  0.12%
 83	   41756	  0.13%
 84	   44728	  0.14%
 85	   48590	  0.15%
 86	   51975	  0.16%
 87	   55618	  0.18%
 88	   59981	  0.19%
 89	   63351	  0.20%
 90	   68066	  0.22%
 91	   71951	  0.23%
 92	   76460	  0.24%
 93	   81264	  0.26%
 94	   86289	  0.27%
 95	   90450	  0.29%
 96	   95182	  0.30%
 97	   99707	  0.32%
 98	  103049	  0.33%
 99	  107729	  0.34%
100	  111927	  0.35%
101	  116085	  0.37%
102	  120407	  0.38%
103	  124713	  0.39%
104	  128384	  0.41%
105	  132166	  0.42%
106	  134938	  0.43%
107	  137349	  0.43%
108	  140794	  0.44%
109	  142342	  0.45%
110	  146153	  0.46%
111	  148252	  0.47%
112	  151845	  0.48%
113	  154649	  0.49%
114	  156555	  0.49%
115	  157477	  0.50%
116	  158251	  0.50%
117	  160378	  0.51%
118	  161599	  0.51%
119	  162313	  0.51%
120	  161756	  0.51%
121	  165419	  0.52%
122	  164928	  0.52%
123	  167058	  0.53%
124	  167674	  0.53%
125	  169403	  0.54%
126	  167434	  0.53%
127	  169281	  0.53%
128	  169463	  0.54%
129	  168650	  0.53%
130	  169285	  0.54%
131	  169464	  0.54%
132	  170529	  0.54%
133	  171711	  0.54%
134	  171433	  0.54%
135	  171036	  0.54%
136	  169917	  0.54%
137	  170211	  0.54%
138	  167373	  0.53%
139	  168076	  0.53%
140	  167923	  0.53%
141	  167670	  0.53%
142	  165912	  0.52%
143	  166563	  0.53%
144	  168635	  0.53%
145	  173375	  0.55%
146	  174282	  0.55%
147	  201097	  0.64%
148	  159257	  0.50%
149	  158173	  0.50%
150	22035924	 69.64%
31641312 reads passed initial QC


criterion=sequence-density
sequence-density=0.36
sequence-density-rank=1
fanout-score=4.10
fanout-score-rank=30
prefix-density=0.47
prefix-fanout=3.1
sequence=ATCTGCAGCTCCAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=394.48
fanout-score-rank=1
prefix-density=0.32
prefix-fanout=13.6
sequence=TCTTCCTCCACTCCAAGCCAAATATAATTAAATAAACAAATAAAAAAACTCTTCATGTCAATAATAGCAACTGTTATTTGCGCTATATACTTTTGGACTGGTTATCCAAGGGAAGGAGATTGCAAGAGTTCAACACATTCTACACAACAGCACGCAGCATACATGTCTGCTGCCCTTGGAGTTTACAGCACAGAGTAATCCGTCTCATACACATTATTCACAGTTTCTGTGTTGCCAGAGTCAGCTGACCTTCCCATTTTGGAGGGTTGTTTGAATAGAGTCCCACTGTATTTCATGACAGTACCATAGTATCTCCTTCCATTGGCCTTGCCTTGTTCTTCTTGTTCATGCTTCCTCAAGTCTCTACCTGGATACCTTGTGTTAATCCTTTCTGAGTCATCTGTACTTCCACTGGGCCTCCTGAGCCACCCAAGTAGGCCAGGTTGGTCTGGCAGGCAGTCTCATTGGCATGTTCCTTCTGTCCTTGATACCATCTTTTAATTCCTGTCAG


criterion=sequence-density
sequence-density=0.52
sequence-density-rank=1
fanout-score=4.86
fanout-score-rank=29
prefix-density=0.68
prefix-fanout=3.7
sequence=GTGGAGCTGCAGAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=296.28
fanout-score-rank=1
prefix-density=0.22
prefix-fanout=7.6
sequence=GAGAAGAAACCTGCGGCCAAGAAGGCTGGCAGTGATGCTGCTGCCTCCCGGCCCCGGGCCGCCAAAGTAGCCAAAAAGGTTCACCCTAAGGGTAAAAAGCCCAAGAAGGCGAAGCCCCATTGCAGCCGAAACCCTGTCCTGGTGAGAGGAATTGGCAGGTACTCCCGATCTGCTATGTACTCCAGGAAGGCCTTGTACAAAAGGAAATACTCGGCTGCCAAGACCAAGGTTGAGAAGAAGAAGGGCCGTTCTGCCATCAACGAGGTGGTGACCCGAGAATACACCATCAACATTCACAAGCGCATC
SRR25183864 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 21 07:23:49
                             Started mapping on |	Jun 21 07:23:49
                                    Finished on |	Jun 21 07:26:42
       Mapping speed, Million of reads per hour |	658.43

                          Number of input reads |	31641312
                      Average input read length |	281
                                    UNIQUE READS:
                   Uniquely mapped reads number |	29863701
                        Uniquely mapped reads % |	94.38%
                          Average mapped length |	280.70
                       Number of splices: Total |	28970751
            Number of splices: Annotated (sjdb) |	28551770
                       Number of splices: GT/AG |	28621134
                       Number of splices: GC/AG |	284310
                       Number of splices: AT/AC |	25123
               Number of splices: Non-canonical |	40184
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1316539
             % of reads mapped to multiple loci |	4.16%
        Number of reads mapped to too many loci |	87698
             % of reads mapped to too many loci |	0.28%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.09%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	461292	461292	461292
N_multimapping	1316539	1316539	1316539
N_noFeature	3247553	28212207	3861072
N_ambiguous	1539945	24774	510751
UnstrandedReadsAssigned:25076203 PositiveStrandReadsAssigned:1626720 NegativeStrandReadsAssigned:25491878
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=129 echo kmer=125
SRR25183864 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25183864-trimmed-pair1.fastq
                             SRR25183864-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,641,312 reads, 26,537,184 reads pseudoaligned
[quant] estimated average fragment length: 209.377
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,140 rounds

 109283 SRR25183864.ke.tsv
  52636 SRR25183864.se.tsv
 161919 total
==> SRR25183864.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25183864.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25183864 completed mapping pipeline successfully
