Starting /dee2/code/volunteer_pipeline.sh SRR25183863
    current disk space = 1248046908
    free memory = 200871316 
SRR25183863 SRAfilesize
906cef85c5879a7a018ba1f768a5104e  SRR25183863.sra
SRR25183863.sra file validated
SRR25183863 is paired end
SRR25183863 is conventional basespace
SRR25183863 read1 length is 39-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25183863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.152	37.0	37.0	37.0	37.0	37.0
2	36.188	37.0	37.0	37.0	37.0	37.0
3	36.3685	37.0	37.0	37.0	37.0	37.0
4	36.29	37.0	37.0	37.0	37.0	37.0
5	36.2695	37.0	37.0	37.0	37.0	37.0
6	36.2225	37.0	37.0	37.0	37.0	37.0
7	36.2745	37.0	37.0	37.0	37.0	37.0
8	36.383	37.0	37.0	37.0	37.0	37.0
9	36.3555	37.0	37.0	37.0	37.0	37.0
10-14	36.3197	37.0	37.0	37.0	37.0	37.0
15-19	36.289	37.0	37.0	37.0	37.0	37.0
20-24	36.3208	37.0	37.0	37.0	37.0	37.0
25-29	36.2315	37.0	37.0	37.0	37.0	37.0
30-34	36.110699999999994	37.0	37.0	37.0	37.0	37.0
35-39	36.1594	37.0	37.0	37.0	37.0	37.0
40-44	36.05692413598647	37.0	37.0	37.0	37.0	37.0
45-49	36.07865030839162	37.0	37.0	37.0	37.0	37.0
50-54	36.034136788777765	37.0	37.0	37.0	37.0	37.0
55-59	36.01511511511511	37.0	37.0	37.0	37.0	37.0
60-64	35.867456152940306	37.0	37.0	37.0	37.0	37.0
65-69	35.79295230720511	37.0	37.0	37.0	37.0	37.0
70-74	35.72044863135414	37.0	37.0	37.0	37.0	37.0
75-79	35.7995191816773	37.0	37.0	37.0	37.0	37.0
80-84	35.78024891659639	37.0	37.0	37.0	37.0	37.0
85-89	35.70612893202245	37.0	37.0	37.0	37.0	37.0
90-94	35.64733554022574	37.0	37.0	37.0	37.0	37.0
95-99	35.60759964511264	37.0	37.0	37.0	37.0	37.0
100-104	35.57364588596628	37.0	37.0	37.0	37.0	37.0
105-109	35.50711660064247	37.0	37.0	37.0	37.0	37.0
110-114	35.41831964380568	37.0	37.0	37.0	34.6	37.0
115-119	35.25216317508715	37.0	37.0	37.0	27.4	37.0
120-124	35.39825110184109	37.0	37.0	37.0	34.6	37.0
125-129	35.2294477344992	37.0	37.0	37.0	27.4	37.0
130-134	35.13231043211623	37.0	37.0	37.0	25.0	37.0
135-139	34.936867390983956	37.0	37.0	37.0	25.0	37.0
140-144	34.43400081212002	37.0	37.0	37.0	25.0	37.0
145-149	34.49417533161545	37.0	37.0	37.0	25.0	37.0
150	34.112320248736886	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	5.0
26	4.0
27	9.0
28	27.0
29	31.0
30	64.0
31	72.0
32	99.0
33	146.0
34	231.0
35	546.0
36	2593.0
37	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.05	8.425	15.4	39.125
2	26.674999999999997	12.275	32.475	28.575
3	27.400000000000002	18.4	23.799999999999997	30.4
4	28.1	28.249999999999996	20.200000000000003	23.45
5	28.725	30.225	22.975	18.075
6	19.975	35.275	25.775	18.975
7	13.850000000000001	22.575	43.475	20.1
8	15.85	25.4	35.575	23.175
9	18.325	23.025000000000002	35.475	23.175
10-14	20.49	31.135	27.015	21.36
15-19	21.12	27.644999999999996	28.689999999999998	22.545
20-24	20.485	26.995	29.515	23.005
25-29	20.115	26.665	29.455	23.765
30-34	20.775	28.065	29.310000000000002	21.85
35-39	21.88	28.560000000000002	27.92	21.64
40-44	21.333533413365345	26.95078031212485	28.356342537014807	23.359343737494996
45-49	20.184128890223157	28.43490443310317	28.46492544781347	22.916041228860202
50-54	21.36923230907817	27.3946551896707	29.001100990891803	22.235011510359325
55-59	20.675675675675677	28.373373373373372	28.048048048048045	22.902902902902902
60-64	22.20219318011116	28.21090581342947	27.084272194682292	22.502628811777075
65-69	21.146809683725127	27.01117738459225	28.850684176231766	22.991328755450855
70-74	19.458834179952724	28.531911683347584	27.440527083438116	24.56872705326158
75-79	21.58855351635573	28.28252186662622	27.483694827847714	22.645229789170333
80-84	20.737233338424723	27.99755613257981	28.562700473499312	22.702510055496155
85-89	22.393723398800063	28.46520691246603	26.778113942874725	22.362955745859185
90-94	22.187029440780933	27.628641154784777	27.768835349706634	22.41549405472766
95-99	20.879062895736595	28.561629379485016	27.870409455466444	22.688898269311945
100-104	21.019624804022275	28.410012434448827	27.366599989187435	23.20376277234146
105-109	20.67496542185339	26.976486860304288	29.134163208852005	23.214384508990317
110-114	21.55483095932727	27.30392998112236	28.007551055431612	23.133688004118756
115-119	21.081560283687946	27.5354609929078	29.131205673758863	22.25177304964539
120-124	22.03410624463256	25.33431480799902	28.769476137897193	23.86210280947123
125-129	20.665219611848826	27.866445352400408	28.04519918283963	23.423135852911134
130-134	20.820021299254527	26.870340788072415	29.625931842385516	22.683706070287542
135-139	20.34075104311544	26.48122392211405	29.005563282336578	24.172461752433936
140-144	20.4123112659698	27.700348432055748	29.25377468060395	22.6335656213705
145-149	21.393336874857706	28.75464825073993	26.8498140699704	23.002200804431965
150	21.492421298095607	29.887291099883406	27.08900116595414	21.53128643606685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	2.5
22	3.5
23	3.0
24	7.5
25	19.5
26	44.0
27	62.0
28	82.0
29	89.5
30	77.5
31	61.0
32	50.5
33	61.5
34	78.5
35	93.5
36	117.5
37	136.0
38	117.0
39	102.0
40	121.0
41	133.0
42	148.5
43	205.5
44	206.5
45	162.5
46	174.5
47	187.0
48	180.0
49	140.5
50	105.0
51	113.5
52	122.0
53	105.0
54	79.0
55	72.0
56	78.5
57	67.0
58	51.5
59	71.5
60	80.0
61	49.0
62	25.0
63	24.0
64	19.5
65	13.0
66	9.0
67	13.0
68	14.0
69	11.5
70	10.5
71	15.0
72	16.0
73	7.5
74	3.0
75	0.5
76	0.0
77	1.5
78	1.5
79	0.0
80	0.0
81	0.5
82	0.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	1.0
40-44	1.0
45-49	1.0
50-54	1.0
55-59	1.0
60-64	3.0
65-69	7.0
70-74	14.0
75-79	33.0
80-84	26.0
85-89	37.0
90-94	61.0
95-99	81.0
100-104	85.0
105-109	94.0
110-114	124.0
115-119	119.0
120-124	123.0
125-129	131.0
130-134	135.0
135-139	119.0
140-144	115.0
145-149	115.0
150-151	2573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.400000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.92238267148014	37.075
2	17.328519855595665	19.2
3	6.4981949458483745	10.8
4	3.2942238267148016	7.3
5	1.7599277978339352	4.875
6	1.4891696750902528	4.95
7	0.7671480144404332	2.9749999999999996
8	0.4061371841155234	1.7999999999999998
9	0.315884476534296	1.575
>10	1.2184115523465704	9.45
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	27	0.675	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	24	0.6	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	20	0.5	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	16	0.4	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	16	0.4	No Hit
GTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCTAACCT	16	0.4	No Hit
GGCAACTACAGTGATGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAA	16	0.4	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	15	0.375	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	14	0.35000000000000003	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	14	0.35000000000000003	No Hit
CGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCTAACCTTG	14	0.35000000000000003	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	14	0.35000000000000003	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	14	0.35000000000000003	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	13	0.325	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	13	0.325	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	13	0.325	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	12	0.3	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	12	0.3	No Hit
GTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAGGACA	11	0.27499999999999997	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	11	0.27499999999999997	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	11	0.27499999999999997	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	11	0.27499999999999997	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	11	0.27499999999999997	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	10	0.25	No Hit
TGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGCA	10	0.25	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	10	0.25	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	10	0.25	No Hit
GGCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGG	9	0.22499999999999998	No Hit
GCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATA	9	0.22499999999999998	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	9	0.22499999999999998	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	9	0.22499999999999998	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	9	0.22499999999999998	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	9	0.22499999999999998	No Hit
GCCGATTTAATAAGTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCAC	9	0.22499999999999998	No Hit
GCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAAT	8	0.2	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	8	0.2	No Hit
CAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGAGC	8	0.2	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	8	0.2	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	8	0.2	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	8	0.2	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	8	0.2	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	8	0.2	No Hit
GAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATA	8	0.2	No Hit
CTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGT	7	0.17500000000000002	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	7	0.17500000000000002	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	7	0.17500000000000002	No Hit
CGGAGTTGTCTTCTAGGACCTTTGCGTAAGGCATAGGGACATCAGCACCA	7	0.17500000000000002	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	7	0.17500000000000002	No Hit
CGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATAGCT	7	0.17500000000000002	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	7	0.17500000000000002	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	7	0.17500000000000002	No Hit
CGCAGATTTATTACCTCACATTCAATTCCCTCCTTAGACAATACAGCTGC	7	0.17500000000000002	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	7	0.17500000000000002	No Hit
GTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCACTTACATCTTACC	7	0.17500000000000002	No Hit
CGCTGCAGCTCCACCTCATGGGCCTCTCGAAGCAGGGCCTGGTGATCTTC	7	0.17500000000000002	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	7	0.17500000000000002	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	7	0.17500000000000002	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	7	0.17500000000000002	No Hit
CTTCATATCAATTAGCACCTGTTTATAAAGGTGCTTCTTGGTTGGTTGGA	7	0.17500000000000002	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	7	0.17500000000000002	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	6	0.15	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	6	0.15	No Hit
GCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGTG	6	0.15	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	6	0.15	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	6	0.15	No Hit
CTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCA	6	0.15	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	6	0.15	No Hit
CTCCACCTCATGGGCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTG	6	0.15	No Hit
TTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGC	6	0.15	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	6	0.15	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	6	0.15	No Hit
TGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTC	6	0.15	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	6	0.15	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	6	0.15	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	6	0.15	No Hit
GGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAG	6	0.15	No Hit
CAGAACTTTATTGAGGGGCGGGGCTCCGACATCTGCACAGCTACAACTGA	6	0.15	No Hit
GTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTA	6	0.15	No Hit
GCCACTATATACAAACACAGGACAACCTGAAGCTAAATGGATGCCCACCG	6	0.15	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	6	0.15	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	6	0.15	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	6	0.15	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	6	0.15	No Hit
GGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGCAA	6	0.15	No Hit
ATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCT	6	0.15	No Hit
GGCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGT	6	0.15	No Hit
CTTATTTTGCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTAC	6	0.15	No Hit
GCTCGTTCTCGGGGCTCAATCATATTTCTCTTCTGGAAGCTCTTGAACCT	6	0.15	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	6	0.15	No Hit
CCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCAC	6	0.15	No Hit
AGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGA	6	0.15	No Hit
CTTCCACAGGCCTCTGCTAACCTTGTATGCACCGTCATACTGGGCAACTT	6	0.15	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	6	0.15	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	5	0.125	No Hit
TAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTT	5	0.125	No Hit
GGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGACCA	5	0.125	No Hit
CCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAG	5	0.125	No Hit
CCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAG	5	0.125	No Hit
TTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGC	5	0.125	No Hit
GCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCA	5	0.125	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	5	0.125	No Hit
GCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCC	5	0.125	No Hit
GCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATTCTTGTGGACTGTA	5	0.125	No Hit
CCGCTATATACAAACACAGGACAACCTGAAGCTAAATGGATGCCCACCGC	5	0.125	No Hit
CTCTTTCTCACGCTGCAGCTCCACCTCATGGGCCTCTCGAAGCAGGGCCT	5	0.125	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	5	0.125	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	5	0.125	No Hit
GTATTTCTTCCACAGGCCTCTGCTAACCTTGTATGCACCGTCATACTGGG	5	0.125	No Hit
TGCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACT	5	0.125	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	5	0.125	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	5	0.125	No Hit
AGAAAATGTAAACTTAAAATCGGCTATAAGATATTTAAGAAATTATATTT	5	0.125	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	5	0.125	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	5	0.125	No Hit
GGATTCGTATGTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGC	5	0.125	No Hit
CAACTACAGTGATGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATA	5	0.125	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	5	0.125	No Hit
ATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTT	5	0.125	No Hit
GCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGGC	5	0.125	No Hit
GGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGG	5	0.125	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	5	0.125	No Hit
GGGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATC	5	0.125	No Hit
CTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTA	5	0.125	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	5	0.125	No Hit
TAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTT	5	0.125	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	5	0.125	No Hit
GCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTG	5	0.125	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	5	0.125	No Hit
CCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTC	5	0.125	No Hit
GCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAA	5	0.125	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	5	0.125	No Hit
CTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCT	10	0.009392008	130.35	6
TCCTCTT	10	0.009392008	130.35	7
ATGATCC	10	0.009392008	130.35	3
CTCTTGT	10	0.009392008	130.35	9
TGATCCT	10	0.009392008	130.35	4
GATGATC	10	0.009392008	130.35	2
CCTCTTG	10	0.009392008	130.35	8
GATCCTC	10	0.009392008	130.35	5
CGATGAT	10	0.009392008	130.35	1
>>END_MODULE
SRR25183863 read2 length is 39-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25183863_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1615	37.0	37.0	37.0	37.0	37.0
2	36.36	37.0	37.0	37.0	37.0	37.0
3	36.3575	37.0	37.0	37.0	37.0	37.0
4	36.2255	37.0	37.0	37.0	37.0	37.0
5	36.442	37.0	37.0	37.0	37.0	37.0
6	36.4175	37.0	37.0	37.0	37.0	37.0
7	36.3345	37.0	37.0	37.0	37.0	37.0
8	36.296	37.0	37.0	37.0	37.0	37.0
9	36.379	37.0	37.0	37.0	37.0	37.0
10-14	36.3584	37.0	37.0	37.0	37.0	37.0
15-19	36.335699999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.350699999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.2723	37.0	37.0	37.0	37.0	37.0
30-34	36.24589999999999	37.0	37.0	37.0	37.0	37.0
35-39	36.1783	37.0	37.0	37.0	37.0	37.0
40-44	36.255999942957224	37.0	37.0	37.0	37.0	37.0
45-49	36.24096706847295	37.0	37.0	37.0	37.0	37.0
50-54	36.16704913570063	37.0	37.0	37.0	37.0	37.0
55-59	36.12742742742743	37.0	37.0	37.0	37.0	37.0
60-64	36.01116723896859	37.0	37.0	37.0	37.0	37.0
65-69	36.050375157253384	37.0	37.0	37.0	37.0	37.0
70-74	35.93958075631942	37.0	37.0	37.0	37.0	37.0
75-79	35.8872587352286	37.0	37.0	37.0	37.0	37.0
80-84	35.814532323410276	37.0	37.0	37.0	37.0	37.0
85-89	35.74442764916472	37.0	37.0	37.0	37.0	37.0
90-94	35.65744046884525	37.0	37.0	37.0	37.0	37.0
95-99	35.64659815584285	37.0	37.0	37.0	37.0	37.0
100-104	35.5034913238126	37.0	37.0	37.0	37.0	37.0
105-109	35.50463312941406	37.0	37.0	37.0	37.0	37.0
110-114	35.445771024961935	37.0	37.0	37.0	37.0	37.0
115-119	35.29418218291229	37.0	37.0	37.0	29.8	37.0
120-124	35.076704660059946	37.0	37.0	37.0	25.0	37.0
125-129	35.09011483354392	37.0	37.0	37.0	25.0	37.0
130-134	34.80406402356622	37.0	37.0	37.0	25.0	37.0
135-139	34.59907009178794	37.0	37.0	37.0	25.0	37.0
140-144	34.58796515688804	37.0	37.0	37.0	25.0	37.0
145-149	34.30960990307526	37.0	37.0	37.0	25.0	37.0
150	34.5203125	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	4.0
26	5.0
27	12.0
28	12.0
29	14.0
30	41.0
31	61.0
32	66.0
33	123.0
34	294.0
35	776.0
36	2315.0
37	276.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.725	18.425	14.924999999999999	29.925
2	24.175	29.2	28.275	18.35
3	24.025	33.225	22.025	20.724999999999998
4	28.349999999999998	33.875	20.200000000000003	17.575
5	29.525000000000002	34.300000000000004	18.95	17.224999999999998
6	22.45	38.275	20.325	18.95
7	21.925	20.125	36.025	21.925
8	23.375	23.625	26.174999999999997	26.825
9	24.725	23.125	28.7	23.45
10-14	25.405	28.4	24.349999999999998	21.845
15-19	25.25	26.375	25.945	22.43
20-24	24.02	28.01	25.095	22.875
25-29	24.060000000000002	26.07	26.195	23.674999999999997
30-34	23.32	26.8	27.150000000000002	22.73
35-39	23.95	26.545	27.255000000000003	22.25
40-44	23.76450580232093	26.810724289715886	26.12545018007203	23.299319727891156
45-49	23.836685679975982	25.73801661162814	27.47923546482538	22.946062243570502
50-54	23.67130417375638	26.398758882994695	27.274547092383145	22.655389850865777
55-59	22.66766766766767	26.22122122122122	28.263263263263262	22.84784784784785
60-64	23.769465725301686	26.47839367082269	26.783836563016372	22.96830404085925
65-69	23.853440930279184	26.62523181795399	27.54247907373064	21.978848178036188
70-74	23.70366644872504	26.630790122214957	27.234320776542774	22.431222652517228
75-79	23.666514990646643	26.558471105718183	28.065119571262446	21.709894332372716
80-84	24.469222544676953	26.33776284303243	27.0760144595489	22.117000152741713
85-89	23.135706226279616	27.633603446507333	27.341265770848295	21.889424556364755
90-94	23.31463591980887	28.34735639347668	26.352965617534018	21.98504206918043
95-99	23.319261213720317	28.105540897097626	28.332453825857517	20.24274406332454
100-104	23.725606789556192	29.250229742148225	27.012270933563975	20.011892534731608
105-109	23.458496930819	29.336946303157664	26.494497594425702	20.710059171597635
110-114	22.677111327119903	28.43501629595746	28.057636228486476	20.83023614843616
115-119	22.40401653868872	28.57058476077968	27.554636739515654	21.470761961015945
120-124	23.55356705074773	28.43834273106153	27.51899975484187	20.489090463348862
125-129	23.657696722356842	28.433873230455298	27.362581303405175	20.54584874378268
130-134	23.401143464964765	29.66360856269113	26.406063023534106	20.52918494881
135-139	23.91832766164317	29.48121397319258	26.38377665115633	20.216681714007915
140-144	22.835788404324795	30.31710325810899	27.400043538204777	19.447064799361442
145-149	22.649279341111875	30.80912071989629	26.75970411042477	19.78189582856707
150	22.34375	31.093749999999996	28.046875	18.515625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	1.0
19	1.0
20	1.0
21	2.5
22	5.0
23	14.0
24	17.0
25	17.0
26	38.5
27	70.5
28	84.0
29	84.0
30	72.5
31	56.5
32	54.0
33	61.0
34	87.5
35	100.0
36	86.5
37	82.0
38	85.0
39	97.0
40	127.5
41	156.5
42	169.0
43	169.5
44	168.0
45	159.5
46	142.5
47	127.0
48	118.0
49	104.5
50	83.0
51	84.0
52	108.5
53	120.5
54	108.0
55	84.5
56	62.5
57	45.5
58	43.0
59	72.5
60	80.0
61	44.0
62	25.5
63	25.5
64	24.0
65	30.5
66	32.0
67	40.0
68	40.5
69	32.0
70	50.5
71	97.5
72	93.5
73	41.0
74	14.0
75	3.0
76	1.0
77	0.5
78	0.5
79	0.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	1.0
40-44	1.0
45-49	1.0
50-54	1.0
55-59	1.0
60-64	3.0
65-69	7.0
70-74	14.0
75-79	33.0
80-84	26.0
85-89	38.0
90-94	61.0
95-99	80.0
100-104	84.0
105-109	93.0
110-114	125.0
115-119	118.0
120-124	121.0
125-129	131.0
130-134	135.0
135-139	119.0
140-144	131.0
145-149	116.0
150-151	2560.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.95992876224399	36.475
2	18.032056990204808	20.25
3	7.92520035618878	13.350000000000001
4	3.6064113980409616	8.1
5	2.3597506678539624	6.625
6	1.0240427426536063	3.45
7	0.6233303650934996	2.45
8	0.17809439002671415	0.8
9	0.3116651825467498	1.575
>10	0.9795191451469278	6.925000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	21	0.525	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	20	0.5	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	16	0.4	No Hit
GTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCA	16	0.4	No Hit
AAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGT	15	0.375	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	13	0.325	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	13	0.325	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	13	0.325	No Hit
GGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAA	12	0.3	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	12	0.3	No Hit
CAAGAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCC	12	0.3	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	12	0.3	No Hit
GGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGTGGAG	11	0.27499999999999997	No Hit
CCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCA	11	0.27499999999999997	No Hit
GTAATATCTTGGCATATTTAGAAATACTTGTGACTGGCTTCAAAATATAA	10	0.25	No Hit
GCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGG	10	0.25	No Hit
GGATCATCGACACCCCCATCTCAGAGATGGGCTTTGCTGGAATTGCTGTT	10	0.25	No Hit
CACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACA	10	0.25	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	10	0.25	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	10	0.25	No Hit
GGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGA	10	0.25	No Hit
CGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCC	10	0.25	No Hit
GTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACT	9	0.22499999999999998	No Hit
GAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGC	9	0.22499999999999998	No Hit
GCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGA	9	0.22499999999999998	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	9	0.22499999999999998	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	9	0.22499999999999998	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	9	0.22499999999999998	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	9	0.22499999999999998	No Hit
TAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGA	8	0.2	No Hit
AGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTG	8	0.2	No Hit
CTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGC	8	0.2	No Hit
GGAAGAGGAGGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAG	8	0.2	No Hit
CTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCG	7	0.17500000000000002	No Hit
GAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTC	7	0.17500000000000002	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	7	0.17500000000000002	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	7	0.17500000000000002	No Hit
GGGCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCG	7	0.17500000000000002	No Hit
AAGAGGAGGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAG	7	0.17500000000000002	No Hit
GTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACCCTCATG	7	0.17500000000000002	No Hit
GAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGC	7	0.17500000000000002	No Hit
GGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACA	7	0.17500000000000002	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	7	0.17500000000000002	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	7	0.17500000000000002	No Hit
GCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCA	7	0.17500000000000002	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	7	0.17500000000000002	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	7	0.17500000000000002	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	6	0.15	No Hit
GTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCG	6	0.15	No Hit
GAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTC	6	0.15	No Hit
GTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGA	6	0.15	No Hit
CTTTTAAGTGTCTATGCATATATTTGTATTATACAATTCAGAATATACTA	6	0.15	No Hit
AAGACATCCTCGCACATCAGGTCCCTAATGCCAAGAAGCTCAGGCGAAAG	6	0.15	No Hit
CTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACC	6	0.15	No Hit
GCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCC	6	0.15	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	6	0.15	No Hit
GCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGG	6	0.15	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	6	0.15	No Hit
CAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATT	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
CAAAGACATCCTCGCACATCAGGTCCCTAATGCCAAGAAGCTCAGGCGAA	6	0.15	No Hit
CTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCA	6	0.15	No Hit
CTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCC	6	0.15	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	6	0.15	No Hit
GGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCC	6	0.15	No Hit
ATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGA	6	0.15	No Hit
GTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGG	6	0.15	No Hit
CGAGGATGCAAAAGGACTTATTAAATCGGCCATTCGTGATAATAACCCAG	6	0.15	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	6	0.15	No Hit
GAAGAGGAGGAGGAAGAGGAAGAAGAGGAGGAGGAAGAGGAGGAAGATGA	6	0.15	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	5	0.125	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	5	0.125	No Hit
GAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACT	5	0.125	No Hit
CCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCC	5	0.125	No Hit
CTCAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTC	5	0.125	No Hit
GAAAATTATTTAATATAAGGACTGGTTAGAACATTATATATATATCCCAT	5	0.125	No Hit
CTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCG	5	0.125	No Hit
GAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCACAA	5	0.125	No Hit
CAGAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCT	5	0.125	No Hit
AAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAA	5	0.125	No Hit
CCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCC	5	0.125	No Hit
TAAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTA	5	0.125	No Hit
CAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTT	5	0.125	No Hit
ATTTGTGCCAGAATTATGGAAGGCCCTGCATTCAACTTCCTTGATGCCCC	5	0.125	No Hit
CCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCCC	5	0.125	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	5	0.125	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	5	0.125	No Hit
CAAAAAGTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACC	5	0.125	No Hit
GAAGAAGAGGAGGAGGAAGAGGAGGAAGATGAATAAAAGCTCTGTCTCCT	5	0.125	No Hit
GTGAAAATTATTTAATATAAGGACTGGTTAGAACATTATATATATATCCC	5	0.125	No Hit
CAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTG	5	0.125	No Hit
TGAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTG	5	0.125	No Hit
GGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTC	5	0.125	No Hit
GCATGATGCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTC	5	0.125	No Hit
CACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTC	5	0.125	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	5	0.125	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	5	0.125	No Hit
CTCAGCTGCCAAGACTTACTATATGTCCGCTGGCCTTCAGCCTGTGCCCA	5	0.125	No Hit
GATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTC	5	0.125	No Hit
CAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGA	5	0.125	No Hit
TCAGCCTGTGCCCATAGTATTCAGGGGGCCCAATGGCGCCTCAGCAGGTG	5	0.125	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	5	0.125	No Hit
AAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCC	5	0.125	No Hit
GGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTT	5	0.125	No Hit
GAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGC	5	0.125	No Hit
CAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGG	5	0.125	No Hit
GCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGC	5	0.125	No Hit
GAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGG	5	0.125	No Hit
AGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGG	5	0.125	No Hit
CTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCC	5	0.125	No Hit
GTTTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGATGGTATT	5	0.125	No Hit
GTTCAAGATGAGCAATCAGACTTATGTTTTATTTTTATCTGGGTAACATA	5	0.125	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	5	0.125	No Hit
GGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACAT	5	0.125	No Hit
GATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGC	5	0.125	No Hit
ATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGCTGTGA	5	0.125	No Hit
CCAGTGGTAATGCTAGAAAATGAACTGATGTATGGAGTTGCATTTGAACT	5	0.125	No Hit
GGAAGAGGAAGAAGAGGAGGAGGAAGAGGAGGAAGATGAATAAAAGCTCT	5	0.125	No Hit
AAGAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCA	5	0.125	No Hit
GTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGCATATATT	5	0.125	No Hit
GCCTTTTGGAGGGGGCCGTAGCTCAGGGCGTGCACTGTGAGGCTGGACCT	5	0.125	No Hit
CTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTAT	5	0.125	No Hit
GGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3953010 spots for SRR25183863.sra
Written 3953010 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
Read 3953008 spots for SRR25183863.sra
Written 3953008 spots for SRR25183863.sra
SRR ids: ['SRR25183863.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_geuqkjav
SRR25183863.sra spots: 31624066
blocks: [[1, 3953008], [3953009, 7906016], [7906017, 11859024], [11859025, 15812032], [15812033, 19765040], [19765041, 23718048], [23718049, 27671056], [27671057, 31624066]]
SRR25183863 file size 10075900
SRR25183863 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25183863 SRR25183863_1.fastq SRR25183863_2.fastq
Input file:	SRR25183863_1.fastq
Paired file:	SRR25183863_2.fastq
trimmed:	SRR25183863-trimmed-pair1.fastq, SRR25183863-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun Jun 21 07:41:16 2026 >> started

Sun Jun 21 07:41:48 2026 >> done (31.774s)
31624066 read pairs processed; of these:
      15 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
31624051 (100.00%) read pairs available; of these:
  196309 ( 0.62%) trimmed read pairs available after processing
31427742 (99.38%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       9	  0.00%
 19	       6	  0.00%
 20	      18	  0.00%
 21	      17	  0.00%
 22	      27	  0.00%
 23	      38	  0.00%
 24	       9	  0.00%
 25	      43	  0.00%
 26	      19	  0.00%
 27	      25	  0.00%
 28	     141	  0.00%
 29	      68	  0.00%
 30	     135	  0.00%
 31	      26	  0.00%
 32	      45	  0.00%
 33	       3	  0.00%
 34	     158	  0.00%
 35	    1339	  0.00%
 36	     857	  0.00%
 37	     820	  0.00%
 38	     963	  0.00%
 39	    4729	  0.01%
 40	     875	  0.00%
 41	     810	  0.00%
 42	     865	  0.00%
 43	    1064	  0.00%
 44	    1158	  0.00%
 45	    1044	  0.00%
 46	    1343	  0.00%
 47	    1287	  0.00%
 48	    1599	  0.01%
 49	    1550	  0.00%
 50	    2324	  0.01%
 51	    2342	  0.01%
 52	    2401	  0.01%
 53	    2446	  0.01%
 54	    2615	  0.01%
 55	    3859	  0.01%
 56	    3245	  0.01%
 57	    3844	  0.01%
 58	    4067	  0.01%
 59	    4417	  0.01%
 60	    4915	  0.02%
 61	    5438	  0.02%
 62	    5924	  0.02%
 63	    6448	  0.02%
 64	    7137	  0.02%
 65	    7852	  0.02%
 66	    8620	  0.03%
 67	    9447	  0.03%
 68	   10857	  0.03%
 69	   11622	  0.04%
 70	   13283	  0.04%
 71	   14438	  0.05%
 72	   15605	  0.05%
 73	   17266	  0.05%
 74	   18971	  0.06%
 75	   20683	  0.07%
 76	   22959	  0.07%
 77	   25014	  0.08%
 78	   26986	  0.09%
 79	   30158	  0.10%
 80	   32607	  0.10%
 81	   35547	  0.11%
 82	   39482	  0.12%
 83	   42779	  0.14%
 84	   45888	  0.15%
 85	   48802	  0.15%
 86	   52579	  0.17%
 87	   56330	  0.18%
 88	   60028	  0.19%
 89	   64886	  0.21%
 90	   68712	  0.22%
 91	   72995	  0.23%
 92	   78163	  0.25%
 93	   82248	  0.26%
 94	   87767	  0.28%
 95	   92096	  0.29%
 96	   96122	  0.30%
 97	  100794	  0.32%
 98	  105448	  0.33%
 99	  109237	  0.35%
100	  113993	  0.36%
101	  118057	  0.37%
102	  122981	  0.39%
103	  126096	  0.40%
104	  130695	  0.41%
105	  132025	  0.42%
106	  136417	  0.43%
107	  140488	  0.44%
108	  142377	  0.45%
109	  145649	  0.46%
110	  147989	  0.47%
111	  150675	  0.48%
112	  153671	  0.49%
113	  156167	  0.49%
114	  157670	  0.50%
115	  160689	  0.51%
116	  160853	  0.51%
117	  162224	  0.51%
118	  164125	  0.52%
119	  164281	  0.52%
120	  165227	  0.52%
121	  166536	  0.53%
122	  167447	  0.53%
123	  168552	  0.53%
124	  170020	  0.54%
125	  170780	  0.54%
126	  170947	  0.54%
127	  172146	  0.54%
128	  170422	  0.54%
129	  170897	  0.54%
130	  171409	  0.54%
131	  170932	  0.54%
132	  171401	  0.54%
133	  174232	  0.55%
134	  172087	  0.54%
135	  172396	  0.55%
136	  172299	  0.54%
137	  172080	  0.54%
138	  169734	  0.54%
139	  170193	  0.54%
140	  168900	  0.53%
141	  168353	  0.53%
142	  167579	  0.53%
143	  168141	  0.53%
144	  170174	  0.54%
145	  175039	  0.55%
146	  176633	  0.56%
147	  201957	  0.64%
148	  160207	  0.51%
149	  158652	  0.50%
150	21902774	 69.26%
31624051 reads passed initial QC


criterion=sequence-density
sequence-density=0.36
sequence-density-rank=1
fanout-score=4.18
fanout-score-rank=29
prefix-density=0.48
prefix-fanout=3.1
sequence=ATCTGCAGCTCCAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=31
fanout-score=414.37
fanout-score-rank=1
prefix-density=0.33
prefix-fanout=14.2
sequence=TCTTCCTCCACTCCAAGCCAAATATAATTAAATAAACAAATAAAAAAACTCTTCATGTCAATAATAGCAACTGTTATTTGCGCTATATACTTTTGGACTGGTTATCCAAGGGAAGGAGATTGCAAGAGTTCAACACATTCTACACAACAGCACGCAGCATACATGTCTGCTGCCCTTGGAGTTTACAGCACAGAGTAATCCGTCTCATACACATTATTCACAGTTTCTGTGTTGCCAGAGTCAGCTGACCTTCCCATTTTGGAGGGTTGTTTGAATAGAGTCCCACTGTATTTCATGACAGTACCATAGTATCTCCTTCCATTGGCCTTGCCTTGTTCTTCTTGTTCATGCTTCCTCAAGTCTCTACCTGGATACCTTGTGTTAATCCTTTCTGAGTCATCTGTACTTCCACTGGGCCTCCTGAGCCACCCAAGTAGGCCAGGTTGGTCTGGCAGGCAGTCTCATTGGCATGTTCCTTCTGTCCTTGATACCATCTTTTAATTCCTGTCAG


criterion=sequence-density
sequence-density=0.52
sequence-density-rank=1
fanout-score=4.72
fanout-score-rank=25
prefix-density=0.68
prefix-fanout=3.6
sequence=GTGGAGCTGCAGAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=291.93
fanout-score-rank=1
prefix-density=0.22
prefix-fanout=8.5
sequence=GAGAAGAAACCTGCGGCCAAGAAGGCTGGCAGTGATGCTGCTGCCTCCCGGCCCCGGGCCGCCAAAGTAGCCAAAAAGGTTCACCCTAAGGGTAAAAAGCCCAAGAAGGCGAAGCCCCATTGCAGCCGAAACCCTGTCCTGGTGAGAGGAATTGGCAGGTACTCCCGATCTGCTATGTACTCCAGGAAGGCCTTGTACAAAAGGAAATACTCGGCTGCCAAGACCAAGGTTGAGAAGAAGAAGGGCCGTTCTGCCATCAACGAGGTGGTGACCCGAGAATACACCATCAACATTCACAAGCGCATC
SRR25183863 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 21 07:42:09
                             Started mapping on |	Jun 21 07:42:09
                                    Finished on |	Jun 21 07:45:02
       Mapping speed, Million of reads per hour |	658.07

                          Number of input reads |	31624051
                      Average input read length |	280
                                    UNIQUE READS:
                   Uniquely mapped reads number |	29836015
                        Uniquely mapped reads % |	94.35%
                          Average mapped length |	280.41
                       Number of splices: Total |	28899172
            Number of splices: Annotated (sjdb) |	28480607
                       Number of splices: GT/AG |	28552000
                       Number of splices: GC/AG |	281781
                       Number of splices: AT/AC |	25433
               Number of splices: Non-canonical |	39958
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1320498
             % of reads mapped to multiple loci |	4.18%
        Number of reads mapped to too many loci |	87136
             % of reads mapped to too many loci |	0.28%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.11%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	467721	467721	467721
N_multimapping	1320498	1320498	1320498
N_noFeature	3244987	28184628	3861287
N_ambiguous	1535405	24186	509056
UnstrandedReadsAssigned:25055623 PositiveStrandReadsAssigned:1627201 NegativeStrandReadsAssigned:25465672
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=129 echo kmer=125
SRR25183863 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25183863-trimmed-pair1.fastq
                             SRR25183863-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,624,051 reads, 26,528,225 reads pseudoaligned
[quant] estimated average fragment length: 207.007
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,234 rounds

 109283 SRR25183863.ke.tsv
  52636 SRR25183863.se.tsv
 161919 total
==> SRR25183863.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8	0	0
ENSMUST00000196221.1	9	1	0	0
ENSMUST00000179664.1	11	3	0	0
ENSMUST00000178537.1	12	4	0	0
ENSMUST00000178862.1	14	6	0	0
ENSMUST00000179520.1	11	3	0	0
ENSMUST00000179883.1	16	8	0	0
ENSMUST00000195858.1	10	2	0	0
ENSMUST00000179932.1	12	4	0	0

==> SRR25183863.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25183863 completed mapping pipeline successfully
