Starting /dee2/code/volunteer_pipeline.sh SRR25183862
    current disk space = 428935687168
    free memory = 394333028 
SRR25183862 SRAfilesize
4a6f7c17b4e316d0e51e31610bb8fdce  SRR25183862.sra
SRR25183862.sra file validated
SRR25183862 is paired end
SRR25183862 is conventional basespace
SRR25183862 read1 length is 43-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25183862_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	43-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.286	37.0	37.0	37.0	37.0	37.0
2	36.1825	37.0	37.0	37.0	37.0	37.0
3	36.307	37.0	37.0	37.0	37.0	37.0
4	36.285	37.0	37.0	37.0	37.0	37.0
5	36.2915	37.0	37.0	37.0	37.0	37.0
6	36.276	37.0	37.0	37.0	37.0	37.0
7	36.24975	37.0	37.0	37.0	37.0	37.0
8	36.33525	37.0	37.0	37.0	37.0	37.0
9	36.407	37.0	37.0	37.0	37.0	37.0
10-14	36.4022	37.0	37.0	37.0	37.0	37.0
15-19	36.3351	37.0	37.0	37.0	37.0	37.0
20-24	36.3317	37.0	37.0	37.0	37.0	37.0
25-29	36.2819	37.0	37.0	37.0	37.0	37.0
30-34	36.1863	37.0	37.0	37.0	37.0	37.0
35-39	36.176199999999994	37.0	37.0	37.0	37.0	37.0
40-44	36.13685988997249	37.0	37.0	37.0	37.0	37.0
45-49	36.04751340411391	37.0	37.0	37.0	37.0	37.0
50-54	36.02886144912451	37.0	37.0	37.0	37.0	37.0
55-59	36.01564550509894	37.0	37.0	37.0	37.0	37.0
60-64	35.93093900562325	37.0	37.0	37.0	37.0	37.0
65-69	35.86574000473639	37.0	37.0	37.0	37.0	37.0
70-74	35.75321677769675	37.0	37.0	37.0	37.0	37.0
75-79	35.84733373373739	37.0	37.0	37.0	37.0	37.0
80-84	35.84550900673406	37.0	37.0	37.0	37.0	37.0
85-89	35.74609345967578	37.0	37.0	37.0	37.0	37.0
90-94	35.61230620974892	37.0	37.0	37.0	37.0	37.0
95-99	35.59566618452766	37.0	37.0	37.0	37.0	37.0
100-104	35.537062458517546	37.0	37.0	37.0	37.0	37.0
105-109	35.54201037620637	37.0	37.0	37.0	37.0	37.0
110-114	35.4411822254181	37.0	37.0	37.0	34.6	37.0
115-119	35.31500060976295	37.0	37.0	37.0	29.8	37.0
120-124	35.329001855389166	37.0	37.0	37.0	34.6	37.0
125-129	35.161694905634434	37.0	37.0	37.0	25.0	37.0
130-134	35.082965110232784	37.0	37.0	37.0	25.0	37.0
135-139	34.940474620698	37.0	37.0	37.0	25.0	37.0
140-144	34.55696274749994	37.0	37.0	37.0	25.0	37.0
145-149	33.97917818044863	37.0	37.0	37.0	25.0	37.0
150	33.8420233463035	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	7.0
26	8.0
27	18.0
28	16.0
29	37.0
30	50.0
31	79.0
32	105.0
33	127.0
34	173.0
35	560.0
36	2655.0
37	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.475	8.05	15.55	38.925
2	27.675	12.125	32.9	27.3
3	28.675	17.150000000000002	23.400000000000002	30.775000000000002
4	28.15	28.999999999999996	19.8	23.05
5	28.075	30.225	23.599999999999998	18.099999999999998
6	20.849999999999998	34.8	24.9	19.45
7	13.50337584396099	21.48037009252313	44.61115278819705	20.40510127531883
8	14.003500875218805	24.781195298824706	36.509127281820454	24.706176544136035
9	17.75	23.075000000000003	34.699999999999996	24.474999999999998
10-14	20.815	29.925	27.845	21.415
15-19	21.14	26.935	29.165000000000003	22.759999999999998
20-24	19.86	27.195000000000004	29.959999999999997	22.985
25-29	19.96	26.91	29.315	23.815
30-34	21.105	28.155	28.765	21.975
35-39	20.77	28.485	28.775000000000002	21.97
40-44	21.6110805540277	26.736336816840844	28.31641582079104	23.336166808340415
45-49	19.770931279383817	27.943383014904473	29.583875162548768	22.70181054316295
50-54	21.77221526908636	27.44430538172716	28.51564455569462	22.267834793491865
55-59	19.7012231802687	28.373771806697413	28.619410467214756	23.30559454581913
60-64	21.841445057701957	28.449573507275467	27.19518314099348	22.5137982940291
65-69	21.123787505654118	26.80806151681158	29.692918530431722	22.375232447102576
70-74	20.446527567785505	27.24523737526459	27.688741054329203	24.619494002620705
75-79	21.460702552438715	27.879706848622693	28.081880212282034	22.57771038665656
80-84	20.69315987397093	28.392113019615817	28.285394857200934	22.629332249212318
85-89	21.648216482164823	28.11090610906109	27.900779007790078	22.34009840098401
90-94	22.895202414403162	27.19325632219794	27.281715058799044	22.629826204599855
95-99	21.58033826638478	28.541226215644823	27.73784355179704	22.14059196617336
100-104	20.78467251493461	28.055540605995372	27.743393789354716	23.4163930897153
105-109	20.35686664456966	26.94729864103414	28.836592641697052	23.85924207269915
110-114	21.6974674880219	26.88227241615332	28.54209445585216	22.87816563997262
115-119	20.88450637694851	27.072508266414737	29.050543221539915	22.992442135096837
120-124	22.108926608865552	26.089876663817318	28.214678226889728	23.586518500427403
125-129	21.07964265348793	27.250839510866122	27.61832351264018	24.051194323005763
130-134	21.02862431414028	25.907318040589672	29.27877305480267	23.785284590467377
135-139	21.25319869977177	26.530188809737883	27.173386817898887	25.04322567259147
140-144	20.75580974444364	26.431622384710057	29.312966046477957	23.499601824368348
145-149	20.748428149382622	28.937201727141883	27.013105067797895	23.3012650556776
150	21.90661478599222	26.186770428015567	28.249027237354085	23.65758754863813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	3.5
22	6.5
23	3.5
24	4.5
25	17.5
26	44.5
27	65.0
28	76.0
29	83.0
30	72.5
31	56.0
32	52.5
33	54.0
34	65.0
35	89.0
36	114.0
37	123.0
38	105.0
39	96.0
40	144.5
41	163.5
42	142.5
43	194.5
44	230.5
45	177.5
46	151.5
47	188.5
48	197.0
49	153.5
50	131.0
51	129.0
52	116.5
53	102.5
54	76.0
55	61.0
56	75.5
57	67.5
58	46.5
59	59.0
60	75.5
61	56.5
62	25.0
63	20.0
64	21.0
65	14.5
66	9.0
67	11.0
68	9.5
69	6.5
70	8.0
71	12.5
72	12.0
73	5.0
74	1.5
75	1.0
76	1.0
77	1.5
78	2.0
79	1.5
80	0.5
81	0.5
82	1.0
83	0.5
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.025
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	1.0
45-49	2.0
50-54	5.0
55-59	4.0
60-64	7.0
65-69	9.0
70-74	9.0
75-79	17.0
80-84	26.0
85-89	50.0
90-94	63.0
95-99	62.0
100-104	77.0
105-109	117.0
110-114	117.0
115-119	108.0
120-124	107.0
125-129	138.0
130-134	133.0
135-139	142.0
140-144	111.0
145-149	125.0
150-151	2570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.974999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.46806610093792	38.324999999999996
2	16.39124609200536	18.35
3	6.2974542206342115	10.575
4	2.7244305493523897	6.1
5	2.099151406878071	5.875
6	1.205895489057615	4.05
7	0.7146047342563644	2.8000000000000003
8	0.3573023671281822	1.6
9	0.49129075480125056	2.475
>10	1.2505582849486379	9.85
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	24	0.6	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	21	0.525	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	19	0.475	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	18	0.44999999999999996	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	18	0.44999999999999996	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	17	0.42500000000000004	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	16	0.4	No Hit
GGCAACTACAGTGATGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAA	16	0.4	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	16	0.4	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	14	0.35000000000000003	No Hit
GTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCTAACCT	14	0.35000000000000003	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	13	0.325	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	13	0.325	No Hit
CTGGCATCACAGGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTG	13	0.325	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	13	0.325	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	13	0.325	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	12	0.3	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	12	0.3	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	12	0.3	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	12	0.3	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	12	0.3	No Hit
CTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCTGTATCTCAAA	11	0.27499999999999997	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	11	0.27499999999999997	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	11	0.27499999999999997	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	11	0.27499999999999997	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	11	0.27499999999999997	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	11	0.27499999999999997	No Hit
TTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGC	10	0.25	No Hit
GATTGTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAG	9	0.22499999999999998	No Hit
GGCCTCTCGAAGCAGGGCCTGGTGATCTTCAAAGGTTGGGTTGTAGGAGG	9	0.22499999999999998	No Hit
CTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCA	9	0.22499999999999998	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	9	0.22499999999999998	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	9	0.22499999999999998	No Hit
GGGTAACATCATTTAGTCTGTATCATTGGTATGTATTTGCAAAACATTTT	9	0.22499999999999998	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	9	0.22499999999999998	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	9	0.22499999999999998	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	9	0.22499999999999998	No Hit
GCCAGAAACATCTTCAAATGCCTTGTCACACCAACAGCAAAGTGCACTGA	9	0.22499999999999998	No Hit
CTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTA	9	0.22499999999999998	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	8	0.2	No Hit
TTGTTATCTTATTTTGCTGAATTTGAGACAAAGAATCTAGTTGCTTCACT	8	0.2	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	8	0.2	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	8	0.2	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	8	0.2	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	8	0.2	No Hit
CAGCGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATA	8	0.2	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	8	0.2	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	7	0.17500000000000002	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	7	0.17500000000000002	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	7	0.17500000000000002	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	7	0.17500000000000002	No Hit
TCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTG	7	0.17500000000000002	No Hit
GCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTG	7	0.17500000000000002	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	7	0.17500000000000002	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	7	0.17500000000000002	No Hit
GGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAG	7	0.17500000000000002	No Hit
CGGCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCG	7	0.17500000000000002	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	7	0.17500000000000002	No Hit
CCGCATTCTTTTTCTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACC	7	0.17500000000000002	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	7	0.17500000000000002	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	7	0.17500000000000002	No Hit
GCTCCGGACACCCACGGCCACTTCATGTAAACCACTGACACTTTGAGAAA	7	0.17500000000000002	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	7	0.17500000000000002	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	6	0.15	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	6	0.15	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	6	0.15	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	6	0.15	No Hit
CCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAG	6	0.15	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	6	0.15	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	6	0.15	No Hit
CTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGA	6	0.15	No Hit
GCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCA	6	0.15	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	6	0.15	No Hit
GCATCCTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCC	6	0.15	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	6	0.15	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	6	0.15	No Hit
CGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATAGCT	6	0.15	No Hit
GGCAACTTTAGCAGGACCCTTGGCACCATCAAACTTCCCTTTAGACTTAT	6	0.15	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	6	0.15	No Hit
CGCAGATTTATTACCTCACATTCAATTCCCTCCTTAGACAATACAGCTGC	6	0.15	No Hit
GTTGGACTAAGGTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTG	6	0.15	No Hit
CGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCTAACCTTG	6	0.15	No Hit
GTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCC	6	0.15	No Hit
CTCCGGACACCCACGGCCACTTCATGTAAACCACTGACACTTTGAGAAAC	6	0.15	No Hit
TGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTA	6	0.15	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	6	0.15	No Hit
CTTCATATCAATTAGCACCTGTTTATAAAGGTGCTTCTTGGTTGGTTGGA	6	0.15	No Hit
GGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAA	6	0.15	No Hit
GCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAA	6	0.15	No Hit
GCCGATTTAATAAGTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCAC	6	0.15	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	5	0.125	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	5	0.125	No Hit
CGCCAAACAAGGAGCATCAAGAATCTGTCTGGGTTGTTTCATTCTCTTTA	5	0.125	No Hit
CAGGGTTTGTGGATTTGATCTTGGGGCGGAATTCAGAGCAGAATAAGAAA	5	0.125	No Hit
CCGGGTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTAC	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	5	0.125	No Hit
GCCAGGGTTTGTGGATTTGATCTTGGGGCGGAATTCAGAGCAGAATAAGA	5	0.125	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	5	0.125	No Hit
GCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTA	5	0.125	No Hit
GCACCATCAAACTTCCCTTTAGACTTATAGTCAGCAACATCCTTCTCATA	5	0.125	No Hit
GTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAGGACA	5	0.125	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	5	0.125	No Hit
CCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCC	5	0.125	No Hit
CGTATTTCTTCCACAGGCCTCTGCTAACCTTGTATGCACCGTCATACTGG	5	0.125	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	5	0.125	No Hit
TGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTG	5	0.125	No Hit
GTTGGTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATT	5	0.125	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	5	0.125	No Hit
GGGATATATATATAATGTTCTAACCAGTCCTTATATTAAATAATTTTCAC	5	0.125	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	5	0.125	No Hit
CCAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAG	5	0.125	No Hit
GACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCC	5	0.125	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	5	0.125	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	5	0.125	No Hit
CGGCACACAGTCAATGGAATATTTCTTATACCAAATAAATCTTTACAGAT	5	0.125	No Hit
CTCCTCTTCCTCTTCTTCCACCTTTTTCCGGGCAACTTTAGCAGGACCCT	5	0.125	No Hit
TGCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACT	5	0.125	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	5	0.125	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	5	0.125	No Hit
CCTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATAC	5	0.125	No Hit
CCAGAAACATCTTCAAATGCCTTGTCACACCAACAGCAAAGTGCACTGAG	5	0.125	No Hit
GTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCT	5	0.125	No Hit
GGGAGGGCTAAGGAGTCGGGCCTGTGCCTTGCGCACATCCCTGGGCAGTT	5	0.125	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	5	0.125	No Hit
ATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAA	5	0.125	No Hit
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	5	0.125	No Hit
GGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGG	5	0.125	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	5	0.125	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	5	0.125	No Hit
GTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCC	5	0.125	No Hit
TGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTGCA	5	0.125	No Hit
ATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTC	5	0.125	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	5	0.125	No Hit
GCCGCATTCTTTTTCTGCACCATTTTGTCCATCTTCTTGGCAATGCGAAC	5	0.125	No Hit
CCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTC	5	0.125	No Hit
CTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACA	10	0.008592622	134.2875	8
AATTTGA	10	0.008592622	134.2875	4
TTGAGAC	10	0.008592622	134.2875	7
GAATTTG	10	0.008592622	134.2875	3
ATTTGAG	10	0.008592622	134.2875	5
>>END_MODULE
SRR25183862 read2 length is 43-150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25183862_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	43-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.268	37.0	37.0	37.0	37.0	37.0
2	36.4425	37.0	37.0	37.0	37.0	37.0
3	36.4105	37.0	37.0	37.0	37.0	37.0
4	36.4195	37.0	37.0	37.0	37.0	37.0
5	36.5765	37.0	37.0	37.0	37.0	37.0
6	36.4375	37.0	37.0	37.0	37.0	37.0
7	36.3145	37.0	37.0	37.0	37.0	37.0
8	36.4995	37.0	37.0	37.0	37.0	37.0
9	36.4305	37.0	37.0	37.0	37.0	37.0
10-14	36.44840000000001	37.0	37.0	37.0	37.0	37.0
15-19	36.3663	37.0	37.0	37.0	37.0	37.0
20-24	36.403	37.0	37.0	37.0	37.0	37.0
25-29	36.350899999999996	37.0	37.0	37.0	37.0	37.0
30-34	36.339299999999994	37.0	37.0	37.0	37.0	37.0
35-39	36.2881	37.0	37.0	37.0	37.0	37.0
40-44	36.2773615903976	37.0	37.0	37.0	37.0	37.0
45-49	36.25987654992788	37.0	37.0	37.0	37.0	37.0
50-54	36.256961689555546	37.0	37.0	37.0	37.0	37.0
55-59	36.165841560282765	37.0	37.0	37.0	37.0	37.0
60-64	36.149026104629755	37.0	37.0	37.0	37.0	37.0
65-69	36.0623728735334	37.0	37.0	37.0	37.0	37.0
70-74	35.95916771737753	37.0	37.0	37.0	37.0	37.0
75-79	35.93150223212165	37.0	37.0	37.0	37.0	37.0
80-84	35.80247093555934	37.0	37.0	37.0	37.0	37.0
85-89	35.80747891492355	37.0	37.0	37.0	37.0	37.0
90-94	35.67447240960591	37.0	37.0	37.0	37.0	37.0
95-99	35.65741002873368	37.0	37.0	37.0	37.0	37.0
100-104	35.38422755553363	37.0	37.0	37.0	37.0	37.0
105-109	35.4695260296587	37.0	37.0	37.0	37.0	37.0
110-114	35.27047410716173	37.0	37.0	37.0	29.8	37.0
115-119	35.24888695467062	37.0	37.0	37.0	29.8	37.0
120-124	34.94705321666042	37.0	37.0	37.0	25.0	37.0
125-129	34.962490441751285	37.0	37.0	37.0	25.0	37.0
130-134	34.73623521972518	37.0	37.0	37.0	25.0	37.0
135-139	34.48481099997552	37.0	37.0	37.0	25.0	37.0
140-144	34.39027714996264	37.0	37.0	37.0	25.0	37.0
145-149	34.17186502056022	37.0	37.0	37.0	25.0	37.0
150	34.27493156042237	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	4.0
26	8.0
27	7.0
28	15.0
29	14.0
30	32.0
31	41.0
32	76.0
33	144.0
34	282.0
35	819.0
36	2300.0
37	257.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.65	18.775	15.55	30.025000000000002
2	23.5	30.349999999999998	28.1	18.05
3	24.725	32.9	21.7	20.674999999999997
4	29.45	34.599999999999994	18.4	17.549999999999997
5	28.925	34.675	20.325	16.075
6	22.400000000000002	36.975	21.2	19.425
7	21.3	20.375	36.9	21.425
8	22.85	23.075000000000003	26.525	27.55
9	24.7	24.625	26.575	24.099999999999998
10-14	25.46	28.599999999999998	24.32	21.62
15-19	24.855	26.290000000000003	25.945	22.91
20-24	24.135	28.73	24.745	22.39
25-29	23.52	27.275	26.33	22.875
30-34	24.25	26.775	25.955000000000002	23.02
35-39	24.755	26.39	26.640000000000004	22.215
40-44	24.521226061303064	26.32131606580329	26.241312065603278	22.916145807290363
45-49	23.772131639491846	26.242872861858558	27.053115934780436	22.931879563869163
50-54	23.83479349186483	27.48435544430538	26.252816020025033	22.428035043804755
55-59	23.08000802085422	26.744535793061964	26.89993984359334	23.275516342490477
60-64	24.00903161063723	26.477671851480178	26.758655293527344	22.75464124435524
65-69	24.234809267728803	26.411016736191385	27.59712519475298	21.757048801326835
70-74	23.61153109565568	26.443906864227394	27.910492893861505	22.034069146255415
75-79	23.58857720495325	26.64644933030073	27.56633813495072	22.198635329795298
80-84	23.808313853033845	26.31364976115459	27.594267710133142	22.283768675678424
85-89	23.57494361287677	27.383637482058642	27.762969038343243	21.278449866721346
90-94	23.0208712850674	28.413053661583305	26.919273408629575	21.64680164471972
95-99	23.367697594501717	28.220988633359767	28.189267776896642	20.22204599524187
100-104	23.639300134589504	29.415881561238223	27.084791386271874	19.860026917900402
105-109	23.445531420991543	28.950422815453493	26.700934062897254	20.90311170065771
110-114	22.803813003025287	29.339574176608252	26.485529996004338	21.37108282436212
115-119	23.45642540620384	28.738552437223042	26.806499261447563	20.998522895125554
120-124	23.100415343268995	28.964085023210355	27.333251893476668	20.60224774004398
125-129	23.642820903094876	29.394977168949772	26.69330289193303	20.268899036022322
130-134	22.837095386244787	29.80737406500298	26.64327795061892	20.712252598133315
135-139	22.56545227870896	30.108048206122735	26.728078681257795	20.598420833910513
140-144	22.501995501052175	30.839561715405267	26.551048545098322	20.107394238444236
145-149	22.3601065854587	31.77769318614389	26.486486486486488	19.375713741910925
150	24.051622995698082	29.37035588580368	28.705514274540477	17.872506843957765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.0
20	1.5
21	3.5
22	8.5
23	12.5
24	12.0
25	18.0
26	40.0
27	63.0
28	77.0
29	75.5
30	64.0
31	49.0
32	43.0
33	65.0
34	89.5
35	94.5
36	87.5
37	79.5
38	71.5
39	96.0
40	134.5
41	161.0
42	177.5
43	186.5
44	188.5
45	165.0
46	149.5
47	155.0
48	159.0
49	116.0
50	80.0
51	78.0
52	94.5
53	112.0
54	90.0
55	65.0
56	53.5
57	47.0
58	40.0
59	60.5
60	70.0
61	39.0
62	22.0
63	22.5
64	26.0
65	40.5
66	43.0
67	41.0
68	39.5
69	29.0
70	52.5
71	98.5
72	91.5
73	39.0
74	8.0
75	2.5
76	1.5
77	1.0
78	1.0
79	1.5
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	1.0
45-49	2.0
50-54	5.0
55-59	4.0
60-64	7.0
65-69	9.0
70-74	9.0
75-79	17.0
80-84	26.0
85-89	51.0
90-94	63.0
95-99	62.0
100-104	78.0
105-109	118.0
110-114	117.0
115-119	107.0
120-124	106.0
125-129	141.0
130-134	133.0
135-139	142.0
140-144	121.0
145-149	124.0
150-151	2557.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.00000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.92857142857142	35.8
2	19.375	21.7
3	7.455357142857142	12.525
4	4.196428571428571	9.4
5	1.9196428571428572	5.375
6	0.8482142857142857	2.85
7	0.5803571428571429	2.275
8	0.5803571428571429	2.6
9	0.26785714285714285	1.35
>10	0.8482142857142857	6.125
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	18	0.44999999999999996	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	16	0.4	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	16	0.4	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	16	0.4	No Hit
GTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCA	16	0.4	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	14	0.35000000000000003	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	14	0.35000000000000003	No Hit
GGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAA	13	0.325	No Hit
GAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGG	13	0.325	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	12	0.3	No Hit
CAAGAAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCC	12	0.3	No Hit
GAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGC	11	0.27499999999999997	No Hit
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	11	0.27499999999999997	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	11	0.27499999999999997	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	11	0.27499999999999997	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	11	0.27499999999999997	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	10	0.25	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	10	0.25	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	10	0.25	No Hit
GTAATATCTTGGCATATTTAGAAATACTTGTGACTGGCTTCAAAATATAA	9	0.22499999999999998	No Hit
TAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGA	9	0.22499999999999998	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	9	0.22499999999999998	No Hit
CTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGC	9	0.22499999999999998	No Hit
AAGAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGT	9	0.22499999999999998	No Hit
AAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGC	9	0.22499999999999998	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	8	0.2	No Hit
CTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCG	8	0.2	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	8	0.2	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	8	0.2	No Hit
GCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGG	8	0.2	No Hit
CAAGTACCAGGCTCCTGACATTGATGTGCAGCTCAGCTCTGAGTTGTCTG	8	0.2	No Hit
GGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGA	8	0.2	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	8	0.2	No Hit
GATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGC	8	0.2	No Hit
ATAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGCTGTGA	8	0.2	No Hit
CCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCA	8	0.2	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	8	0.2	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	8	0.2	No Hit
TAAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGCTGTGAT	7	0.17500000000000002	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	7	0.17500000000000002	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	7	0.17500000000000002	No Hit
CGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACA	7	0.17500000000000002	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	7	0.17500000000000002	No Hit
AAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCT	7	0.17500000000000002	No Hit
CGGGAGATGAAAGATTATGGACCAGCTAAAGGAGGCAAGAAGAAGAAGGA	7	0.17500000000000002	No Hit
GGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTC	7	0.17500000000000002	No Hit
CACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAA	7	0.17500000000000002	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	7	0.17500000000000002	No Hit
CAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCA	7	0.17500000000000002	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	7	0.17500000000000002	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	7	0.17500000000000002	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	6	0.15	No Hit
AAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCAT	6	0.15	No Hit
CAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGG	6	0.15	No Hit
GGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCA	6	0.15	No Hit
CCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAA	6	0.15	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	6	0.15	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	6	0.15	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	6	0.15	No Hit
GCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGC	6	0.15	No Hit
TGCTCCCAAAGACATCCTCGCACATCAGGTCCCTAATGCCAAGAAGCTCA	6	0.15	No Hit
AAAAGCTCTGTCTCCTTGTGAATACCTTAGAGTAGGAGAGCGCTGTGATT	6	0.15	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	6	0.15	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	6	0.15	No Hit
TGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCG	6	0.15	No Hit
CGAGGATGCAAAAGGACTTATTAAATCGGCCATTCGTGATAATAACCCAG	6	0.15	No Hit
AAAGAACCAAGAAAGAAGAGGACCTTGGTCCAGAAGAAGTCACAGCGTCT	6	0.15	No Hit
CAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCCCG	6	0.15	No Hit
TGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAG	6	0.15	No Hit
GTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACCAGGCTT	6	0.15	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	5	0.125	No Hit
GAAAATTATTTAATATAAGGACTGGTTAGAACATTATATATATATCCCAT	5	0.125	No Hit
CCTACCTCCAATTGATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCAC	5	0.125	No Hit
CAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTA	5	0.125	No Hit
GCAAAATAAGATAACAAACAGTTTTATTACATCATTTGAAAATTACTAGT	5	0.125	No Hit
CAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCC	5	0.125	No Hit
GACAAATCATCTCGTGACTGTGGAAGGAGGCTGGCCACAATTTGGAGTAG	5	0.125	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	5	0.125	No Hit
GAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTC	5	0.125	No Hit
TAAGAATCAGATGGTATTACATTATCCCTAATAATGGGGATAATGCTTTT	5	0.125	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	5	0.125	No Hit
GTTTTATTTTTATCTGGGTAACATATTATTTTGAAATTTTCATCTTAAGT	5	0.125	No Hit
GGGGATAATGCTTTTGAGTGTCAAAAAGTAATACTGCATTCTTCAATTAA	5	0.125	No Hit
GCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGC	5	0.125	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	5	0.125	No Hit
GAAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGC	5	0.125	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	5	0.125	No Hit
GAAGGACCCAAATGCCCCCAAAAGACCTCCGTCTGGATTTTTCTTATTCT	5	0.125	No Hit
ACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCC	5	0.125	No Hit
CCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTAT	5	0.125	No Hit
AAGACATCCTCGCACATCAGGTCCCTAATGCCAAGAAGCTCAGGCGAAAG	5	0.125	No Hit
TAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAGG	5	0.125	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	5	0.125	No Hit
GCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGG	5	0.125	No Hit
GAAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACA	5	0.125	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	5	0.125	No Hit
AAAGGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCC	5	0.125	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	5	0.125	No Hit
CAATGATACAGACTAAATGATGTTACCCTCATGGGAAATATATGATCCTT	5	0.125	No Hit
GTTTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGATGGTATT	5	0.125	No Hit
CTAAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTAT	5	0.125	No Hit
TGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCG	5	0.125	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	5	0.125	No Hit
TGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGC	5	0.125	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	5	0.125	No Hit
GCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAG	5	0.125	No Hit
GGAAGAGGAGGAAGATGAATAAAAGCTCTGTCTCCTTGTGAATACCTTAG	5	0.125	No Hit
TTTGCAAATACATACCAATGATACAGACTAAATGATGTTACCCTCATGGG	5	0.125	No Hit
GTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCC	5	0.125	No Hit
TAAAACCAAATACTGTTCAAGATGAGCAATCAGACTTATGTTTTATTTTT	5	0.125	No Hit
GCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCC	5	0.125	No Hit
GGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCA	5	0.125	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.025	0.0	0.0	0.0	0.0
122-123	0.025	0.0	0.0	0.0	0.0
124-125	0.025	0.0	0.0	0.0	0.0
126-127	0.025	0.0	0.0	0.0	0.0
128-129	0.025	0.0	0.0	0.0	0.0
130-131	0.025	0.0	0.0	0.0	0.0
132-133	0.025	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977077 spots for SRR25183862.sra
Written 1977077 spots for SRR25183862.sra
Read 1977084 spots for SRR25183862.sra
Written 1977084 spots for SRR25183862.sra
SRR ids: ['SRR25183862.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gv3ynff7
SRR25183862.sra spots: 31633239
blocks: [[1, 1977077], [1977078, 3954154], [3954155, 5931231], [5931232, 7908308], [7908309, 9885385], [9885386, 11862462], [11862463, 13839539], [13839540, 15816616], [15816617, 17793693], [17793694, 19770770], [19770771, 21747847], [21747848, 23724924], [23724925, 25702001], [25702002, 27679078], [27679079, 29656155], [29656156, 31633239]]
SRR25183862 file size 1906074
SRR25183862 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25183862 SRR25183862_1.fastq SRR25183862_2.fastq
Input file:	SRR25183862_1.fastq
Paired file:	SRR25183862_2.fastq
trimmed:	SRR25183862-trimmed-pair1.fastq, SRR25183862-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun Jun 21 10:59:08 2026 >> started

Sun Jun 21 11:00:21 2026 >> done (72.329s)
31633239 read pairs processed; of these:
      12 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
31633226 (100.00%) read pairs available; of these:
  186542 ( 0.59%) trimmed read pairs available after processing
31446684 (99.41%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       4	  0.00%
 19	       6	  0.00%
 20	       8	  0.00%
 21	      18	  0.00%
 22	      31	  0.00%
 23	      17	  0.00%
 24	       8	  0.00%
 25	      31	  0.00%
 26	      16	  0.00%
 27	      17	  0.00%
 28	     124	  0.00%
 29	      84	  0.00%
 30	     133	  0.00%
 31	      29	  0.00%
 32	      41	  0.00%
 33	       5	  0.00%
 34	     168	  0.00%
 35	    1183	  0.00%
 36	     778	  0.00%
 37	     803	  0.00%
 38	     883	  0.00%
 39	    4042	  0.01%
 40	     876	  0.00%
 41	     791	  0.00%
 42	     818	  0.00%
 43	    1009	  0.00%
 44	    1197	  0.00%
 45	     958	  0.00%
 46	    1365	  0.00%
 47	    1228	  0.00%
 48	    1487	  0.00%
 49	    1614	  0.01%
 50	    2251	  0.01%
 51	    2296	  0.01%
 52	    2455	  0.01%
 53	    2449	  0.01%
 54	    2697	  0.01%
 55	    3770	  0.01%
 56	    3323	  0.01%
 57	    3669	  0.01%
 58	    4164	  0.01%
 59	    4734	  0.01%
 60	    5106	  0.02%
 61	    5512	  0.02%
 62	    6260	  0.02%
 63	    6620	  0.02%
 64	    7071	  0.02%
 65	    7787	  0.02%
 66	    8572	  0.03%
 67	    9714	  0.03%
 68	   10703	  0.03%
 69	   12260	  0.04%
 70	   13363	  0.04%
 71	   14738	  0.05%
 72	   16093	  0.05%
 73	   17670	  0.06%
 74	   19488	  0.06%
 75	   21157	  0.07%
 76	   23256	  0.07%
 77	   25738	  0.08%
 78	   27790	  0.09%
 79	   30903	  0.10%
 80	   33263	  0.11%
 81	   36362	  0.11%
 82	   39851	  0.13%
 83	   43437	  0.14%
 84	   46656	  0.15%
 85	   50631	  0.16%
 86	   54253	  0.17%
 87	   57474	  0.18%
 88	   61484	  0.19%
 89	   65848	  0.21%
 90	   70601	  0.22%
 91	   75127	  0.24%
 92	   79392	  0.25%
 93	   84496	  0.27%
 94	   89418	  0.28%
 95	   93894	  0.30%
 96	   97758	  0.31%
 97	  103614	  0.33%
 98	  107344	  0.34%
 99	  110847	  0.35%
100	  116441	  0.37%
101	  119742	  0.38%
102	  124774	  0.39%
103	  128942	  0.41%
104	  132823	  0.42%
105	  135765	  0.43%
106	  140009	  0.44%
107	  142017	  0.45%
108	  144582	  0.46%
109	  147005	  0.46%
110	  150563	  0.48%
111	  152961	  0.48%
112	  156136	  0.49%
113	  158464	  0.50%
114	  159068	  0.50%
115	  162762	  0.51%
116	  163020	  0.52%
117	  164292	  0.52%
118	  166545	  0.53%
119	  166879	  0.53%
120	  167321	  0.53%
121	  169243	  0.54%
122	  169758	  0.54%
123	  170434	  0.54%
124	  172809	  0.55%
125	  172956	  0.55%
126	  172478	  0.55%
127	  173865	  0.55%
128	  172698	  0.55%
129	  172264	  0.54%
130	  172771	  0.55%
131	  173496	  0.55%
132	  173006	  0.55%
133	  174652	  0.55%
134	  173870	  0.55%
135	  174243	  0.55%
136	  173720	  0.55%
137	  173564	  0.55%
138	  170997	  0.54%
139	  171910	  0.54%
140	  171579	  0.54%
141	  170166	  0.54%
142	  168054	  0.53%
143	  170418	  0.54%
144	  170978	  0.54%
145	  176237	  0.56%
146	  177714	  0.56%
147	  202202	  0.64%
148	  162461	  0.51%
149	  160591	  0.51%
150	21780850	 68.85%
31633226 reads passed initial QC


criterion=sequence-density
sequence-density=0.36
sequence-density-rank=1
fanout-score=4.28
fanout-score-rank=29
prefix-density=0.48
prefix-fanout=3.2
sequence=ATCTGCAGCTCCAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=394.13
fanout-score-rank=1
prefix-density=0.32
prefix-fanout=12.7
sequence=TCTTCCTCCACTCCAAGCCAAATATAATTAAATAAACAAATAAAAAAACTCTTCATGTCAATAATAGCAACTGTTATTTGCGCTATATACTTTTGGACTGGTTATCCAAGGGAAGGAGATTGCAAGAGTTCAACACATTCTACACAACAGCACGCAGCATACATGTCTGCTGCCCTTGGAGTTTACAGCACAGAGTAATCCGTCTCATACACATTATTCACAGTTTCTGTGTTGCCAGAGTCAGCTGACCTTCCCATTTTGGAGGGTTGTTTGAATAGAGTCCCACTGTATTTCATGACAGTACCATAGTATCTCCTTCCATTGGCCTTGCCTTGTTCTTCTTGTTCATGCTTCCTCAAGTCTCTACCTGGATACCTTGTGTTAATCCTTTCTGAGTCATCTGTACTTCCACTGGGCCTCCTGAGCCACCCAAGTAGGCCAGGTTGGTCTGGCAGGCAGTCTCATTGGCATGTTCCTTCTGTCCTTGATACCATCTTTTAATTCCTGTCAG


criterion=sequence-density
sequence-density=0.52
sequence-density-rank=1
fanout-score=4.84
fanout-score-rank=27
prefix-density=0.69
prefix-fanout=3.6
sequence=GTGGAGCTGCAGAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=335.59
fanout-score-rank=1
prefix-density=0.25
prefix-fanout=7.8
sequence=GGAGAAGAAACCTGCGGCCAAGAAGGCTGGCAGTGATGCTGCTGCCTCCCGGCCCCGGGCCGCCAAAGTAGCCAAAAAGGTTCACCCTAAGGGTAAAAAGCCCAAGAAGGCGAAGCCCCATTGCAGCCGAAACCCTGTCCTGGTGAGAGGAATTGGCAGGTACTCCCGATCTGCTATGTACTCCAGGAAGGCCTTGTACAAAAGGAAATACTCGGCTGCCAAGACCAAGGTTGAGAAGAAGAAGGGCCGTTCTGCCATCAACGAGGTGGTGACCCGAGAATACACCATCAACATTCACAAGCGCATC
SRR25183862 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 21 11:01:09
                             Started mapping on |	Jun 21 11:01:09
                                    Finished on |	Jun 21 11:08:25
       Mapping speed, Million of reads per hour |	261.19

                          Number of input reads |	31633226
                      Average input read length |	280
                                    UNIQUE READS:
                   Uniquely mapped reads number |	29834104
                        Uniquely mapped reads % |	94.31%
                          Average mapped length |	280.15
                       Number of splices: Total |	28884765
            Number of splices: Annotated (sjdb) |	28468081
                       Number of splices: GT/AG |	28536702
                       Number of splices: GC/AG |	282786
                       Number of splices: AT/AC |	25669
               Number of splices: Non-canonical |	39608
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1325390
             % of reads mapped to multiple loci |	4.19%
        Number of reads mapped to too many loci |	87120
             % of reads mapped to too many loci |	0.28%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.13%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	473902	473902	473902
N_multimapping	1325390	1325390	1325390
N_noFeature	3251042	28180146	3874008
N_ambiguous	1530509	23983	508150
UnstrandedReadsAssigned:25052553 PositiveStrandReadsAssigned:1629975 NegativeStrandReadsAssigned:25451946
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=128 echo kmer=123
SRR25183862 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25183862-trimmed-pair1.fastq
                             SRR25183862-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 31,633,226 reads, 26,528,175 reads pseudoaligned
[quant] estimated average fragment length: 205.246
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,186 rounds

 109283 SRR25183862.ke.tsv
  52636 SRR25183862.se.tsv
 161919 total
==> SRR25183862.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	2	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	4	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25183862.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25183862 completed mapping pipeline successfully
