Starting /dee2/code/volunteer_pipeline.sh SRR25194574
    current disk space = 428923787264
    free memory = 393452588 
SRR25194574 SRAfilesize
88eccfb093c1291f0ff7f47e355f0f15  SRR25194574.sra
SRR25194574.sra file validated
SRR25194574 is single end
SRR25194574 is conventional basespace
SRR25194574 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.212	34.0	34.0	34.0	34.0	34.0
2	33.4665	34.0	34.0	34.0	34.0	34.0
3	33.33	34.0	34.0	34.0	34.0	34.0
4	33.459	34.0	34.0	34.0	34.0	34.0
5	33.5325	34.0	34.0	34.0	34.0	34.0
6	33.6005	34.0	34.0	34.0	34.0	34.0
7	33.6465	34.0	34.0	34.0	34.0	34.0
8	33.6225	34.0	34.0	34.0	34.0	34.0
9	33.5785	34.0	34.0	34.0	34.0	34.0
10-11	33.62375	34.0	34.0	34.0	34.0	34.0
12-13	33.60424999999999	34.0	34.0	34.0	34.0	34.0
14-15	33.634	34.0	34.0	34.0	34.0	34.0
16-17	33.682	34.0	34.0	34.0	34.0	34.0
18-19	33.454499999999996	34.0	34.0	34.0	34.0	34.0
20-21	33.61528473091364	34.0	34.0	34.0	34.0	34.0
22-23	33.52643310300421	34.0	34.0	34.0	34.0	34.0
24-25	33.578882139182625	34.0	34.0	34.0	34.0	34.0
26-27	33.62339527851635	34.0	34.0	34.0	34.0	34.0
28-29	33.613943755477806	34.0	34.0	34.0	34.0	34.0
30-31	33.61804555170182	34.0	34.0	34.0	34.0	34.0
32-33	33.54102462876755	34.0	34.0	34.0	34.0	34.0
34-35	33.583781625738276	34.0	34.0	34.0	34.0	34.0
36-37	33.58550003491459	34.0	34.0	34.0	34.0	34.0
38-39	33.60159461151741	34.0	34.0	34.0	34.0	34.0
40-41	33.53282095056714	34.0	34.0	34.0	34.0	34.0
42-43	33.54727334321158	34.0	34.0	34.0	34.0	34.0
44-45	33.57007933926218	34.0	34.0	34.0	34.0	34.0
46-47	33.51659715197526	34.0	34.0	34.0	34.0	34.0
48-49	33.53913840071358	34.0	34.0	34.0	34.0	34.0
50-51	33.53524654394655	34.0	34.0	34.0	34.0	34.0
52-53	33.46487519158658	34.0	34.0	34.0	34.0	34.0
54-55	33.56875535547876	34.0	34.0	34.0	34.0	34.0
56-57	33.478355662514964	34.0	34.0	34.0	34.0	34.0
58-59	33.50807019061696	34.0	34.0	34.0	34.0	34.0
60-61	33.502354273935616	34.0	34.0	34.0	34.0	34.0
62-63	33.48552082961797	34.0	34.0	34.0	34.0	34.0
64-65	33.46340002182504	34.0	34.0	34.0	34.0	34.0
66-67	33.34317722665146	34.0	34.0	34.0	34.0	34.0
68-69	33.35078285389372	34.0	34.0	34.0	34.0	34.0
70-71	33.21334886499914	34.0	34.0	34.0	34.0	34.0
72-73	33.23683610197668	34.0	34.0	34.0	34.0	34.0
74-75	33.23472809667673	34.0	34.0	34.0	34.0	34.0
76-77	33.06436909803281	34.0	34.0	34.0	34.0	34.0
78-79	33.10900668546114	34.0	34.0	34.0	34.0	34.0
80-81	33.02790615902341	34.0	34.0	34.0	34.0	34.0
82-83	33.144702842377264	34.0	34.0	34.0	34.0	34.0
84-85	33.13731663626021	34.0	34.0	34.0	34.0	34.0
86-87	33.206157157484704	34.0	34.0	34.0	34.0	34.0
88-89	33.12517069237012	34.0	34.0	34.0	34.0	34.0
90-91	33.139363633025404	34.0	34.0	34.0	34.0	34.0
92-93	32.97588369520764	34.0	34.0	34.0	34.0	34.0
94-95	32.88884183235233	34.0	34.0	34.0	34.0	34.0
96-97	32.9686955417148	34.0	34.0	34.0	34.0	34.0
98-99	33.036135786938374	34.0	34.0	34.0	34.0	34.0
100-101	32.806065644881436	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	4.0
26	17.0
27	16.0
28	32.0
29	46.0
30	52.0
31	94.0
32	216.0
33	2542.0
34	980.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.78144536134033	21.780445111277817	32.40810202550637	20.030007501875467
2	28.475	28.275	24.275	18.975
3	32.275	27.825	23.474999999999998	16.425
4	28.050000000000004	31.15	22.75	18.05
5	25.3	30.4	26.525	17.775
6	22.025	30.9	28.425	18.65
7	26.450000000000003	25.55	27.250000000000004	20.75
8	25.4	26.25	28.299999999999997	20.05
9	24.675	24.275	28.875	22.175
10-11	24.3125	25.624999999999996	30.55	19.5125
12-13	23.0125	30.3875	28.025	18.575
14-15	20.9	34.137499999999996	31.3125	13.65
16-17	20.8875	30.7625	34.7375	13.612499999999999
18-19	20.866834170854272	30.301507537688444	33.379396984924625	15.452261306532664
20-21	20.712945590994373	32.04502814258912	33.00813008130081	14.233896185115697
22-23	20.07015785517414	32.59834627912804	31.746429466299176	15.585066399398647
24-25	19.312766491096063	31.565086531226488	33.03235515425132	16.089791823426136
26-27	19.947209653092006	30.10306686777275	33.509301156359975	16.440422322775262
28-29	17.541210519692964	32.326664149993704	31.559078897697248	18.57304643261608
30-31	19.727891156462583	31.947593852355755	30.309901738473165	18.014613252708493
32-33	19.430084478628167	33.5140587567772	29.23969234648846	17.816164418106165
34-35	20.97406704617331	32.485768500948765	29.968374446552815	16.57179000632511
36-37	20.761421319796955	31.916243654822335	31.941624365482234	15.380710659898478
38-39	23.173064660119884	33.75844917740084	28.733579900522894	14.334906261956384
40-41	19.39992306705988	36.05590460315425	29.73458135658418	14.809590973201692
42-43	18.661336084601494	35.16894506061388	31.48052618003611	14.689192674748517
44-45	18.955011020355244	34.48722935304032	29.18449371191495	17.373265914689483
46-47	15.777110792117968	38.22262821349341	29.648962547305235	16.351298447083387
48-49	19.058947924927306	33.240814168649216	31.403647898493258	16.296590007930213
50-51	17.87981093855503	35.25995948683322	32.045914922349766	14.814314652261984
52-53	16.61635565312843	37.37650933040615	31.092206366630077	14.914928649835346
54-55	19.030420891790527	31.518266425892485	35.796638422003056	13.654674260313932
56-57	18.712217067341488	31.800927878532264	35.14691410094194	14.33994095318431
58-59	20.762772164508327	30.767041411697736	34.73744129785115	13.732745125942792
60-61	16.620458193491903	27.973150445060558	40.91638698380272	14.490004377644826
62-63	17.052283653846153	27.734375	42.11237980769231	13.100961538461538
64-65	19.896438098226895	27.69496312568649	35.83869449238976	16.569904283696847
66-67	18.21717990275527	29.513776337115072	36.90437601296597	15.364667747163697
68-69	17.952195287336835	30.750974741481606	35.887438548906594	15.409391422274963
70-71	18.467269824374668	31.541600141919464	33.5107326592159	16.48039737448998
72-73	19.074720029373967	30.659078391775292	35.43234808151276	14.833853497337984
74-75	18.597560975609756	35.11814024390244	32.56478658536585	13.719512195121952
76-77	18.552839116719245	33.69479495268139	32.6301261829653	15.122239747634069
78-79	16.174994982942003	36.72486453943408	31.888420630142484	15.211719847481437
80-81	14.978426135196218	29.21717690569139	38.586398191904664	17.217998767207725
82-83	14.061154177433247	35.723514211886304	33.93626184323858	16.27906976744186
84-85	19.826652221018417	34.58288190682557	29.577464788732392	16.01300108342362
86-87	20.1282902012829	29.72793629727936	34.35080734350807	15.79296615792966
88-89	14.600363306085375	32.01634877384196	36.330608537693	17.052679382379655
90-91	19.845722300140253	28.58812529219261	33.076203833567085	18.489948574100048
92-93	14.822848879248014	32.97180043383948	34.73126054470957	17.474090142202943
94-95	18.697582634435125	30.26640355204736	34.13912185495806	16.896891958559447
96-97	16.383744855967077	31.995884773662553	33.43621399176955	18.184156378600825
98-99	16.865134311824978	33.09332594849072	32.01329271669898	18.028247022985322
100-101	7.441130298273155	36.26373626373626	36.95447409733124	19.340659340659343
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	2.5
19	2.0
20	13.5
21	40.0
22	57.5
23	79.0
24	99.5
25	224.5
26	349.0
27	313.0
28	235.0
29	207.0
30	208.0
31	164.5
32	148.5
33	182.5
34	203.5
35	192.5
36	158.5
37	153.5
38	156.5
39	155.0
40	188.5
41	186.0
42	162.0
43	154.0
44	152.5
45	152.5
46	128.0
47	141.5
48	159.5
49	101.5
50	48.0
51	38.5
52	31.5
53	34.5
54	41.5
55	40.0
56	22.0
57	11.0
58	30.5
59	34.5
60	16.5
61	7.5
62	6.0
63	3.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	1.5
72	1.5
73	0.0
74	0.0
75	0.0
76	0.0
77	3.0
78	3.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.5
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.2752654345261502
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	7.0
22-23	4.0
24-25	8.0
26-27	7.0
28-29	3.0
30-31	4.0
32-33	11.0
34-35	14.0
36-37	14.0
38-39	24.0
40-41	16.0
42-43	29.0
44-45	21.0
46-47	43.0
48-49	77.0
50-51	62.0
52-53	50.0
54-55	41.0
56-57	36.0
58-59	79.0
60-61	89.0
62-63	157.0
64-65	78.0
66-67	145.0
68-69	138.0
70-71	98.0
72-73	97.0
74-75	86.0
76-77	62.0
78-79	39.0
80-81	139.0
82-83	9.0
84-85	41.0
86-87	57.0
88-89	61.0
90-91	74.0
92-93	39.0
94-95	76.0
96-97	112.0
98-99	124.0
100-101	1729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.150000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.79941434846266	13.25
2	19.54612005856515	13.350000000000001
3	13.689604685212299	14.025000000000002
4	8.711566617862372	11.899999999999999
5	5.856515373352855	10.0
6	4.465592972181552	9.15
7	2.635431918008785	6.3
8	2.1961932650073206	6.0
9	1.2445095168374818	3.8249999999999997
>10	2.855051244509517	12.2
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	20	0.5	No Hit
AACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	19	0.475	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	18	0.44999999999999996	No Hit
ACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGC	17	0.42500000000000004	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	17	0.42500000000000004	No Hit
CCTCTCCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	16	0.4	No Hit
GAACAACTTGAAGAACAAGAGAAAAGAAAGAATGAGGAAATGCAAAATGT	16	0.4	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	16	0.4	No Hit
GGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	15	0.375	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	15	0.375	No Hit
CTGATCTGTGGGCAATCTAGTGGAGACCTTTTCTCAGTTGAGGTTTCTCT	14	0.35000000000000003	No Hit
CCATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCA	14	0.35000000000000003	No Hit
GTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	12	0.3	No Hit
TGGGGAAATGCAAAATGTCAGAACCTCTTTGATTGCTGAGCAGCAGACCAACTTTAACACAGTCTT	12	0.3	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	12	0.3	No Hit
CTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	12	0.3	No Hit
CGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATT	12	0.3	No Hit
TATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	11	0.27499999999999997	No Hit
ACGATTGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	11	0.27499999999999997	No Hit
ACGGAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCA	11	0.27499999999999997	No Hit
GAGCCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAA	11	0.27499999999999997	No Hit
GGGCCAGCTTATCAAAGTTGTGCACACAATTTGAATTAGCATATTACTTT	11	0.27499999999999997	No Hit
GGACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACAC	11	0.27499999999999997	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	11	0.27499999999999997	No Hit
CAGTACTTGAGAAACCACTGCAGGGTGTTGCTGGATGTTTAAAGCTACCC	11	0.27499999999999997	No Hit
TTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	11	0.27499999999999997	No Hit
CAGGGTTAATCAGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAA	11	0.27499999999999997	No Hit
GGGGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCT	11	0.27499999999999997	No Hit
ATTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTA	10	0.25	No Hit
TATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTG	10	0.25	No Hit
TGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTT	10	0.25	No Hit
CGTGATGGCGCACGCCTTTAATCCCAGCACTCGGGAGGCAGAGACAGGCG	10	0.25	No Hit
TTGCGACTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCT	10	0.25	No Hit
CAAAGCGGGGGCTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCT	10	0.25	No Hit
ATGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
TCGCGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAAC	10	0.25	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
TACTGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGC	10	0.25	No Hit
TTTGCTTTTCTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAA	10	0.25	No Hit
AGGGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCGGCAAGATACT	9	0.22499999999999998	No Hit
TATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGT	9	0.22499999999999998	No Hit
AATTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	9	0.22499999999999998	No Hit
ACCTACCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCA	9	0.22499999999999998	No Hit
TGGAAAAGATAATGCATACTCAACATTGTGAAATTAAAGAACTGAAGCAG	9	0.22499999999999998	No Hit
CCTAAAAAATTCATGATAATAATGAGAGTTACCAGGTGGGTTTGTCAGAG	9	0.22499999999999998	No Hit
GGCCCAGCACCCATATGGCAGCTCACAACCATCTGAATCTTCAATTTCAG	9	0.22499999999999998	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATCGATTGCTT	9	0.22499999999999998	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	9	0.22499999999999998	No Hit
AACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCTTTATA	9	0.22499999999999998	No Hit
TGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTG	9	0.22499999999999998	No Hit
GATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	9	0.22499999999999998	No Hit
TGAGTGACGCGGGGGCTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGC	9	0.22499999999999998	No Hit
AAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACC	9	0.22499999999999998	No Hit
ATGTGTGATGGCACGTGAGAAGACTTTAAGACCACGAGGTGGTTCTTCAA	9	0.22499999999999998	No Hit
CATTCTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCT	9	0.22499999999999998	No Hit
CAGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGT	8	0.2	No Hit
GGGATGGTGCGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGC	8	0.2	No Hit
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	8	0.2	No Hit
AATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	8	0.2	No Hit
CAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAAT	8	0.2	No Hit
CCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAA	8	0.2	No Hit
GCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	8	0.2	No Hit
TAGAAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATC	8	0.2	No Hit
AATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGA	8	0.2	No Hit
TGTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	8	0.2	No Hit
CCGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATG	8	0.2	No Hit
TATAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGT	8	0.2	No Hit
AAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTC	8	0.2	No Hit
TACCAGCGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAG	8	0.2	No Hit
TAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	8	0.2	No Hit
ACATCTCATCAGTCCCAGACCTCTTTTTAAAAGTTTTATTGAGGGGGAAG	8	0.2	No Hit
TTAAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATC	8	0.2	No Hit
GACAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAG	8	0.2	No Hit
TTATGTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAA	8	0.2	No Hit
AGTCACGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAA	8	0.2	No Hit
CCGGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	8	0.2	No Hit
TGGATGAACTCGTAATTCATGGTTGACCTTCAGAAGAAGATATTCATTGT	8	0.2	No Hit
TTGATCTTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCT	8	0.2	No Hit
GCTGAAGGAGTCACGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATG	8	0.2	No Hit
CTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	8	0.2	No Hit
CAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGA	8	0.2	No Hit
ACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTA	8	0.2	No Hit
TTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATT	8	0.2	No Hit
GAGAGCAGAACACAGCAGAAGGAAATGCTGAAGTCTTTACTTGAACAAGA	8	0.2	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTAT	8	0.2	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGA	7	0.17500000000000002	No Hit
TACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	7	0.17500000000000002	No Hit
GGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATT	7	0.17500000000000002	No Hit
AGGAAGCAGAGGCAGGTGAATTTCTGAGCTCCAGGACAGCCAGGGCTACA	7	0.17500000000000002	No Hit
TTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TCCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTT	7	0.17500000000000002	No Hit
GTGCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	7	0.17500000000000002	No Hit
AGAATACAGCAGAAGGAAATGCTGAAGTCTTTACTTGAACAAGAGACCGA	7	0.17500000000000002	No Hit
AGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	7	0.17500000000000002	No Hit
AAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAACAGAAAGAT	7	0.17500000000000002	No Hit
ATGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTA	7	0.17500000000000002	No Hit
TTCTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTAT	7	0.17500000000000002	No Hit
GAAAATCAGCAAAGAATTGACCAGATGCTAGAATCTCATGCCTCAACTAT	7	0.17500000000000002	No Hit
CTGTTCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTT	7	0.17500000000000002	No Hit
TACCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	7	0.17500000000000002	No Hit
TGAGGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATC	7	0.17500000000000002	No Hit
GAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAA	7	0.17500000000000002	No Hit
CACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	7	0.17500000000000002	No Hit
GCGGTTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATG	7	0.17500000000000002	No Hit
ATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCACTTCT	7	0.17500000000000002	No Hit
TTCTCATCTGTGCACTTGATGTTACCATATTTATTTTAAGAGAGCACTAA	7	0.17500000000000002	No Hit
CTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAAT	7	0.17500000000000002	No Hit
GAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	7	0.17500000000000002	No Hit
TGGTTTGTCCTCCAGTGCTGATAAACTACTCTAAAAACAGCTGGCCATTG	7	0.17500000000000002	No Hit
TCGGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	7	0.17500000000000002	No Hit
TTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGC	7	0.17500000000000002	No Hit
TTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	7	0.17500000000000002	No Hit
CAAAACGGCTTTTACTGTGAACTTTTAAAGAATGCTTATGTTACATATGT	7	0.17500000000000002	No Hit
GAACCAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACA	7	0.17500000000000002	No Hit
ATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTA	7	0.17500000000000002	No Hit
CTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	7	0.17500000000000002	No Hit
GACTTGGTCTTTGTTGTTCACCTGACAGTCTGTCTTAAATGCCTTCTGTG	7	0.17500000000000002	No Hit
TAAAAGCGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTA	7	0.17500000000000002	No Hit
GTCTTTGGAGCTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCC	7	0.17500000000000002	No Hit
TTGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	7	0.17500000000000002	No Hit
TACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	7	0.17500000000000002	No Hit
GGGTAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TTGCATCAGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAG	6	0.15	No Hit
TAAGTAGGCTTATTTTCCATTCAGTTCACCAGAGGCTTTTATATTCAGCA	6	0.15	No Hit
AAATACTGAAACCAGGGCCTGGTGAGATGGCTCAGTGGGTAAGAGCACCC	6	0.15	No Hit
GGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CCAGCACTTAGGAAGCAGAGGCAGGTGAATTTCTGAGCTCCAGGACAGCC	6	0.15	No Hit
TGTATGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	6	0.15	No Hit
CCGTCACAACCATCTGTATTGAGATCTGACACACTCTAAACACTCTTCTG	6	0.15	No Hit
GAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	6	0.15	No Hit
GTTCCAGTCCTGACTTCCTTGGTGATGAACAGTAGTATGGAAGTGTAAGCCGAATAAACCCTTTCCTCCCC	6	0.15	No Hit
GCAGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAA	6	0.15	No Hit
AACAGAAGCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACA	6	0.15	No Hit
CAAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATC	6	0.15	No Hit
CTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
CACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	6	0.15	No Hit
GTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	6	0.15	No Hit
CCCAAAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCTTAGT	6	0.15	No Hit
TCAGCAATCACTAATTAAAAAGTGCCCCATAGCCTGATCTGTGGGCAATC	6	0.15	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
GGTTGTTGTTAGCAGTAATAAATGATTCTTTTTCAGAAATATTTAATTTC	6	0.15	No Hit
GTGACTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	6	0.15	No Hit
CCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGC	6	0.15	No Hit
GATACAGCCAGAGAAGATTCTTCAAGCTTAGTTGCGGAACTTCAAGAGAA	6	0.15	No Hit
CGTATATTCATGGTTGACCTTCAGAAGAAGATATTCATTGTATATTAACA	6	0.15	No Hit
CCTATTGGGGACTTTGAATAAACATCATTTATTTCAAATAAAAATTGGGAGAAGTGTTCCCAGGTGATGAT	6	0.15	No Hit
GGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
GAAACCTGCTTGCTCAGGGGTGGAGCTTCCTGCTCATTCGTTCTGCCACG	6	0.15	No Hit
ACGAGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATG	6	0.15	No Hit
TGTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATTGATAT	6	0.15	No Hit
CAAATACTGCTGACAGAACAGATCAGGGTGTATATTATTGTACTTGTAGG	6	0.15	No Hit
GAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	6	0.15	No Hit
CTCCGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	6	0.15	No Hit
TGCAACAGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	6	0.15	No Hit
TGAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	6	0.15	No Hit
GAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGA	6	0.15	No Hit
TCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAAT	6	0.15	No Hit
GGGATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
TTGGGGAAAAACGGCTTTTACTGTGAACTTTTAAAGAATGCTTATGTTAC	6	0.15	No Hit
GGCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTCA	6	0.15	No Hit
TTCAGGGTTGACCTTCAGAAGAAGATATTCATTGTATATTAACATTTAGA	6	0.15	No Hit
ACGGCTGTCTCTGCGGCGCTCCGCCCGCCGCCGGTCCGCCGCTGCTTTGG	6	0.15	No Hit
CAAACAATCATTTACTATTTTGCTTTACTTTAAAGAATATAAATTTACTT	6	0.15	No Hit
GGTAAACTAAACCAAAAAATTCATGATAATAATGAGAGTTACCAGGTGGG	6	0.15	No Hit
TTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGT	6	0.15	No Hit
ACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	6	0.15	No Hit
CTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGA	6	0.15	No Hit
TCGGACAGCTGTAGGTTTTCCTGAGCTTGTGAGATATTTAAAATGTATTT	6	0.15	No Hit
TTCAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAAC	6	0.15	No Hit
AGCCAAAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCTTAG	6	0.15	No Hit
TTTTTTTGGTGATGTGGTGATAATGAAAGAGGAAAACAGACTAGCCACTG	6	0.15	No Hit
CTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCAC	6	0.15	No Hit
AGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGA	6	0.15	No Hit
AACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAA	6	0.15	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
AAAAAGGCATTGAGAAAAGACATCTGACCAGCATCTGGCCTCCATGTGTG	6	0.15	No Hit
TGCCGGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTG	6	0.15	No Hit
CAAGCAACCACATGGTGGCTCACAACCATCTGTAATGAGATTGGATGCCC	6	0.15	No Hit
TTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTG	6	0.15	No Hit
TACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	6	0.15	No Hit
TGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TGGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAA	6	0.15	No Hit
CAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAAC	5	0.125	No Hit
TCTCCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	5	0.125	No Hit
GGTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATC	5	0.125	No Hit
TGTAGATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CCTGGCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAG	5	0.125	No Hit
ACAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGA	5	0.125	No Hit
GTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGC	5	0.125	No Hit
TTTGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTTCAA	5	0.125	No Hit
CAACAAGGCAACATAACAATATACTTCATTGGTGGACTGCACTTACCTTT	5	0.125	No Hit
GAGAATAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGT	5	0.125	No Hit
GCTGGTATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTC	5	0.125	No Hit
AGAGTGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCCTGAG	5	0.125	No Hit
ATTAGAAAAAGTTAAACATCTTGAGAATCAAATAGCCAAAACTCCTGCCT	5	0.125	No Hit
GTCACGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAA	5	0.125	No Hit
ATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGT	5	0.125	No Hit
TGAAAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
TTATTCTAGCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTG	5	0.125	No Hit
TGGGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CATGAGAAGAATCTAATATACTTAAGGCTGAATTAGACAATGTTACATCT	5	0.125	No Hit
CTGACTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	5	0.125	No Hit
TTGGCTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTA	5	0.125	No Hit
TGGTAGGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTC	5	0.125	No Hit
AATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTA	5	0.125	No Hit
GATGGCTTCCACAGGCTTCGAATGTACATGGTATATAGACATACATTCAGGGAAAACATCTATAC	5	0.125	No Hit
AAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTG	5	0.125	No Hit
TACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCA	5	0.125	No Hit
TGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	5	0.125	No Hit
TCAAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAAT	5	0.125	No Hit
TATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
CTGAATTAGACAATGTTACATCTTTGCATCGCCAAGCATATGAAATAGAA	5	0.125	No Hit
TGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTT	5	0.125	No Hit
AAAACTCGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAA	5	0.125	No Hit
CTGCAGCACCAGGCACATGTAGTACACAATGAACATAAAAATAATAAAAC	5	0.125	No Hit
TGCCTGGATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTG	5	0.125	No Hit
GTCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	5	0.125	No Hit
ATACTTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCA	5	0.125	No Hit
ACACGCTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAAT	5	0.125	No Hit
AGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAATT	5	0.125	No Hit
GTTACTGATGCTGTTGAGCCCATGCCGTCTTTGTGTACTGTGTGTACTGT	5	0.125	No Hit
TGGGTAATACATGCCTTTCATCTCAGTAATTAGGAGGCGGAGTCGAATGG	5	0.125	No Hit
CAGTTTTGGCCAGTTTTAAAATATCTCACTGACTTATTCCATTTCTTGAA	5	0.125	No Hit
AAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	5	0.125	No Hit
TGAAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATC	5	0.125	No Hit
AAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGT	5	0.125	No Hit
CAACAGCTGCAGGAGTTGAAACTCAAAGTTTCTGACTTGTCAGACATGAG	5	0.125	No Hit
ATCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAA	5	0.125	No Hit
CTCCATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
ACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTAC	5	0.125	No Hit
GCTGGTATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TGTGACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCTTT	5	0.125	No Hit
GTGACGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAG	5	0.125	No Hit
TTCAAAAGGTATAGGTGGCACTGTTGTCCTTATTCCTCTTTAATTCCAGG	5	0.125	No Hit
TCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTG	5	0.125	No Hit
TCAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAATG	5	0.125	No Hit
ATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCAC	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTC	5	0.125	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
AGGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
TATAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	5	0.125	No Hit
TTGCGGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GGGCTGTACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCA	5	0.125	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	5	0.125	No Hit
TCTCGAGATTGCAAAAGAGTTGGTAGCAGTGTTGTGGGGGAGCACAGTCT	5	0.125	No Hit
ATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTG	5	0.125	No Hit
ACTGAAGTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	5	0.125	No Hit
CATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
TTTGCCTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	5	0.125	No Hit
CTGGTTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAA	5	0.125	No Hit
GAATGGTGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	5	0.125	No Hit
TAGCTGCATCTGGTCTCCATGTGTGCCTGCATCTGGCCTCCATGTGTGCC	5	0.125	No Hit
GATTTTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGA	5	0.125	No Hit
TCACTGGATGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	5	0.125	No Hit
ACATCTCACCAGTCCCAGACCTCTTTTTAAAAGTTTTATTGAGGGGGAAG	5	0.125	No Hit
ATGATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATTA	5	0.125	No Hit
ACTGAATTAGACAATGTTACATCTTTGCATCGCCAAGCATATGAAATAGA	5	0.125	No Hit
TAGCAGGTAGGTGTCTCTGGAAGCAGTGAGTAGAGTCACTGTTTTAATTT	5	0.125	No Hit
TGGAGAGAGAACTTGTTGAGAAAGAGTTATTAGAAAAAGTTAAACATCTTGAGAATCAAATAGC	5	0.125	No Hit
ATTTAGATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCTGT	30	0.002261224	67.552086	86-87
TGGGTCT	35	0.0045329807	56.720116	84-85
TTTGGGT	35	0.004922905	55.585712	82-83
GCTTTGG	35	0.0057770587	53.447803	80-81
>>END_MODULE
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821714 spots for SRR25194574.sra
Written 821714 spots for SRR25194574.sra
Read 821724 spots for SRR25194574.sra
Written 821724 spots for SRR25194574.sra
SRR ids: ['SRR25194574.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_iujywtk7
SRR25194574.sra spots: 13147434
blocks: [[1, 821714], [821715, 1643428], [1643429, 2465142], [2465143, 3286856], [3286857, 4108570], [4108571, 4930284], [4930285, 5751998], [5751999, 6573712], [6573713, 7395426], [7395427, 8217140], [8217141, 9038854], [9038855, 9860568], [9860569, 10682282], [10682283, 11503996], [11503997, 12325710], [12325711, 13147434]]
SRR25194574 file size 386143
SRR25194574 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25194574 SRR25194574_1.fastq
Input file:	SRR25194574_1.fastq
trimmed:	SRR25194574-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Sun Jun 21 08:20:41 2026 >> started

Sun Jun 21 08:20:52 2026 >> done (11.340s)
13147434 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
13147434 (100.00%) reads available; of these:
      87 ( 0.00%) trimmed reads available after processing
13147347 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    6989	  0.05%
 21	    6822	  0.05%
 22	    6806	  0.05%
 23	    6705	  0.05%
 24	    7308	  0.06%
 25	    7771	  0.06%
 26	    8634	  0.07%
 27	   10031	  0.08%
 28	   10827	  0.08%
 29	   12639	  0.10%
 30	   14943	  0.11%
 31	   17398	  0.13%
 32	   19418	  0.15%
 33	   20342	  0.15%
 34	   20819	  0.16%
 35	   21076	  0.16%
 36	   22793	  0.17%
 37	   24208	  0.18%
 38	   26517	  0.20%
 39	   28284	  0.22%
 40	   30388	  0.23%
 41	   31524	  0.24%
 42	   34970	  0.27%
 43	   37897	  0.29%
 44	   39081	  0.30%
 45	   39062	  0.30%
 46	   40101	  0.31%
 47	   40729	  0.31%
 48	   42670	  0.32%
 49	   45124	  0.34%
 50	   47199	  0.36%
 51	   49510	  0.38%
 52	   52794	  0.40%
 53	   56760	  0.43%
 54	   58556	  0.45%
 55	   63959	  0.49%
 56	   77274	  0.59%
 57	   79095	  0.60%
 58	   78097	  0.59%
 59	   74505	  0.57%
 60	   72640	  0.55%
 61	   73673	  0.56%
 62	   75613	  0.58%
 63	   75877	  0.58%
 64	   76383	  0.58%
 65	   77284	  0.59%
 66	   82131	  0.62%
 67	   83722	  0.64%
 68	   85373	  0.65%
 69	   91032	  0.69%
 70	   95252	  0.72%
 71	   97997	  0.75%
 72	   99048	  0.75%
 73	   94235	  0.72%
 74	   94715	  0.72%
 75	   95627	  0.73%
 76	   95401	  0.73%
 77	   96357	  0.73%
 78	  100291	  0.76%
 79	  112777	  0.86%
 80	  140006	  1.06%
 81	  162858	  1.24%
 82	     733	  0.01%
 83	   20110	  0.15%
 84	   76159	  0.58%
 85	   94003	  0.71%
 86	   94600	  0.72%
 87	   98931	  0.75%
 88	  102378	  0.78%
 89	  102537	  0.78%
 90	  100334	  0.76%
 91	  115858	  0.88%
 92	   66199	  0.50%
 93	   69040	  0.53%
 94	   99790	  0.76%
 95	  125708	  0.96%
 96	  154693	  1.18%
 97	  251728	  1.91%
 98	  404310	  3.08%
 99	  263744	  2.01%
100	 1115453	  8.48%
101	 6393209	 48.63%
13147434 reads passed initial QC


criterion=sequence-density
sequence-density=0.60
sequence-density-rank=1
fanout-score=3.91
fanout-score-rank=30
prefix-density=1.83
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=851.40
fanout-score-rank=1
prefix-density=5.41
prefix-fanout=1.0
sequence=CATGATACTGAGTTGGTACTTTTTTTGGTCCCTATTTCACAAGGGTTAGGATAGATTAACCAAGAAAGGACAAGTGACAGACTGAAAGGGGCTGGAAAAACAAAGAGGAAAAGGCCTGTCACTTCTGTATAGTTGATGGTTCCTGTCACAAGCCCAGGTCACAAACAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATGTTAACAAAGGAAGGTGGATAAAATACAGTTTCTACTGCCTAAAGAATTTTGGCTCTATTAAAATGTAAGTGTGTGGCTGG
                                 Started job on |	Jun 21 08:21:09
                             Started mapping on |	Jun 21 08:21:09
                                    Finished on |	Jun 21 08:21:32
       Mapping speed, Million of reads per hour |	2057.86

                          Number of input reads |	13147434
                      Average input read length |	89
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11361828
                        Uniquely mapped reads % |	86.42%
                          Average mapped length |	88.38
                       Number of splices: Total |	1204200
            Number of splices: Annotated (sjdb) |	1193191
                       Number of splices: GT/AG |	1196163
                       Number of splices: GC/AG |	4389
                       Number of splices: AT/AC |	347
               Number of splices: Non-canonical |	3301
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.50
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.16
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1670871
             % of reads mapped to multiple loci |	12.71%
        Number of reads mapped to too many loci |	62266
             % of reads mapped to too many loci |	0.47%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.39%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	114735	114735	114735
N_multimapping	1670871	1670871	1670871
N_noFeature	921898	960335	10457215
N_ambiguous	1002111	136097	525220
UnstrandedReadsAssigned:9437819 PositiveStrandReadsAssigned:10265396 NegativeStrandReadsAssigned:379393
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=83 echo kmer=79
SRR25194574 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194574-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,147,434 reads, 8,642,150 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,067 rounds

 109283 SRR25194574.ke.tsv
  52636 SRR25194574.se.tsv
 161919 total
==> SRR25194574.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194574.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194574 completed mapping pipeline successfully
