Starting /dee2/code/volunteer_pipeline.sh SRR25194573
    current disk space = 428920958976
    free memory = 391226172 
SRR25194573 SRAfilesize
1527e2270e702c1ade05511a8fda2a06  SRR25194573.sra
SRR25194573.sra file validated
SRR25194573 is single end
SRR25194573 is conventional basespace
SRR25194573 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.161	34.0	34.0	34.0	34.0	34.0
2	33.5095	34.0	34.0	34.0	34.0	34.0
3	33.339	34.0	34.0	34.0	34.0	34.0
4	33.369	34.0	34.0	34.0	34.0	34.0
5	33.569	34.0	34.0	34.0	34.0	34.0
6	33.653	34.0	34.0	34.0	34.0	34.0
7	33.6145	34.0	34.0	34.0	34.0	34.0
8	33.5925	34.0	34.0	34.0	34.0	34.0
9	33.7305	34.0	34.0	34.0	34.0	34.0
10-11	33.64775	34.0	34.0	34.0	34.0	34.0
12-13	33.67	34.0	34.0	34.0	34.0	34.0
14-15	33.646	34.0	34.0	34.0	34.0	34.0
16-17	33.615750000000006	34.0	34.0	34.0	34.0	34.0
18-19	33.476	34.0	34.0	34.0	34.0	34.0
20-21	33.628157453726864	34.0	34.0	34.0	34.0	34.0
22-23	33.61907069773152	34.0	34.0	34.0	34.0	34.0
24-25	33.545224659846006	34.0	34.0	34.0	34.0	34.0
26-27	33.63292180960846	34.0	34.0	34.0	34.0	34.0
28-29	33.62773425868664	34.0	34.0	34.0	34.0	34.0
30-31	33.62247373961435	34.0	34.0	34.0	34.0	34.0
32-33	33.59728621632175	34.0	34.0	34.0	34.0	34.0
34-35	33.61434728014417	34.0	34.0	34.0	34.0	34.0
36-37	33.64945466108084	34.0	34.0	34.0	34.0	34.0
38-39	33.62676820275804	34.0	34.0	34.0	34.0	34.0
40-41	33.61720879144591	34.0	34.0	34.0	34.0	34.0
42-43	33.593485985054336	34.0	34.0	34.0	34.0	34.0
44-45	33.60802627160167	34.0	34.0	34.0	34.0	34.0
46-47	33.58096693935584	34.0	34.0	34.0	34.0	34.0
48-49	33.52074283931947	34.0	34.0	34.0	34.0	34.0
50-51	33.56251778574579	34.0	34.0	34.0	34.0	34.0
52-53	33.45817899975524	34.0	34.0	34.0	34.0	34.0
54-55	33.55230539177995	34.0	34.0	34.0	34.0	34.0
56-57	33.55223235489693	34.0	34.0	34.0	34.0	34.0
58-59	33.57845450087092	34.0	34.0	34.0	34.0	34.0
60-61	33.51684572272808	34.0	34.0	34.0	34.0	34.0
62-63	33.54989214175655	34.0	34.0	34.0	34.0	34.0
64-65	33.501523756725305	34.0	34.0	34.0	34.0	34.0
66-67	33.37869756143137	34.0	34.0	34.0	34.0	34.0
68-69	33.45165529606979	34.0	34.0	34.0	34.0	34.0
70-71	33.36879194922244	34.0	34.0	34.0	34.0	34.0
72-73	33.33336497539406	34.0	34.0	34.0	34.0	34.0
74-75	33.27878783661153	34.0	34.0	34.0	34.0	34.0
76-77	33.16999621355547	34.0	34.0	34.0	34.0	34.0
78-79	33.212952053986925	34.0	34.0	34.0	34.0	34.0
80-81	33.21303595587364	34.0	34.0	34.0	34.0	34.0
82-83	33.17998352553542	34.0	34.0	34.0	34.0	34.0
84-85	33.26462732764215	34.0	34.0	34.0	34.0	34.0
86-87	33.115341436960044	34.0	34.0	34.0	34.0	34.0
88-89	33.05848264419828	34.0	34.0	34.0	34.0	34.0
90-91	33.09844170588343	34.0	34.0	34.0	34.0	34.0
92-93	33.05271077612962	34.0	34.0	34.0	34.0	34.0
94-95	32.92165992888802	34.0	34.0	34.0	34.0	34.0
96-97	32.99733539811251	34.0	34.0	34.0	34.0	34.0
98-99	33.07589049133167	34.0	34.0	34.0	34.0	34.0
100-101	32.656433772025025	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	3.0
25	6.0
26	6.0
27	7.0
28	31.0
29	43.0
30	48.0
31	90.0
32	232.0
33	2587.0
34	947.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.031757939484873	21.680420105026258	32.98324581145287	18.30457614403601
2	26.6	26.875	26.224999999999998	20.3
3	27.500000000000004	26.325	30.075000000000003	16.1
4	26.575	27.200000000000003	27.575	18.65
5	24.224999999999998	31.275	27.474999999999998	17.025000000000002
6	27.500000000000004	29.125	24.525	18.85
7	23.875	27.675	27.950000000000003	20.5
8	25.6	26.174999999999997	29.225	19.0
9	24.5	25.374999999999996	29.45	20.674999999999997
10-11	22.6	25.6125	31.424999999999997	20.3625
12-13	23.9125	31.900000000000002	29.3875	14.799999999999999
14-15	21.625	33.0125	31.05	14.3125
16-17	19.775000000000002	31.45	35.05	13.725000000000001
18-19	19.21967130849329	32.50533182787605	34.11115292936896	14.163843934261699
20-21	18.617154288572145	31.545386346586646	33.708427106776696	16.129032258064516
22-23	20.873482668001504	28.832436491052434	34.05080715805281	16.243273682893257
24-25	19.606614883487847	30.28063142069657	35.053871210223	15.058882485592584
26-27	19.798994974874372	29.447236180904525	33.50502512562814	17.248743718592966
28-29	19.447597427166098	29.95333585571951	33.29549754067347	17.303569176440913
30-31	17.675360850848314	32.6538364142821	31.03317295517853	18.63762977969106
32-33	20.59496567505721	30.536486142893466	32.151029748283754	16.717518433765573
34-35	19.193980359648005	32.18977171279174	31.297028440249967	17.319219487310292
36-37	21.44411727051594	32.454231212392784	30.124183843297914	15.977467673793369
38-39	21.866151866151867	32.625482625482626	30.797940797940797	14.71042471042471
40-41	20.46337043748382	31.206316334455085	33.43256536370696	14.89774786435413
42-43	19.181652332551472	34.72765181131092	30.896533750325776	15.19416210581183
44-45	17.431794333683108	33.840503672612805	32.52885624344176	16.19884575026233
46-47	19.618745035742652	35.58379666401906	28.24993380990204	16.547524490336247
48-49	17.92946225023468	36.54284564838407	31.420142148317016	14.107549953064236
50-51	17.054580100721385	33.98666122226759	33.70083027085886	15.257928406152171
52-53	17.2404181184669	34.45296167247387	33.728222996515676	14.578397212543553
54-55	16.751484308736217	35.22759400621996	33.48883234379418	14.532089341249646
56-57	18.586349978537704	29.861210473601375	38.08842466733438	13.464014880526543
58-59	19.249781913346904	32.94562372782786	35.69351555684792	12.11107880197732
60-61	19.054652880354507	29.143279172821266	36.76514032496308	15.036927621861151
62-63	17.57066462948816	27.838044308632544	40.50420168067227	14.087089381207027
64-65	18.814229249011856	26.814229249011856	39.17786561264822	15.193675889328063
66-67	17.416096448354512	30.791788856304986	34.457478005865106	17.3346366894754
68-69	19.892110586648684	30.461901550910316	34.8111935266352	14.834794335805798
70-71	19.263954921641133	32.558549040324	33.15724599401303	15.020250044021836
72-73	16.051188299817184	33.49177330895795	34.97257769652651	15.484460694698354
74-75	18.03767953739974	32.30740533482559	33.98619660511098	15.668718522663683
76-77	16.83566099100823	33.61392768318347	33.93916204323703	15.611249282571265
78-79	17.902563099197806	31.59851301115242	33.692036783408334	16.80688710624144
80-81	17.573559048770658	30.854494155582422	36.094316807738814	15.4776299879081
82-83	17.257001647446458	32.72240527182866	34.43163097199341	15.588962108731467
84-85	17.676245606781062	32.99565846599132	32.768244779822204	16.559851147405418
86-87	19.30567315834039	32.790008467400504	31.541066892464016	16.36325148179509
88-89	17.37619461337967	31.82015638575152	32.862728062554304	17.94092093831451
90-91	19.161275931296007	31.16216819094356	31.095248717376755	18.581307160383673
92-93	16.771929824561404	32.70175438596491	32.514619883040936	18.01169590643275
94-95	17.3850228750301	29.15964363111004	33.855044546111245	19.600288947748616
96-97	17.33132681213945	32.8567845497868	33.559066967644846	16.252821670428894
98-99	17.445652173913043	30.16304347826087	31.90217391304348	20.48913043478261
100-101	7.203257124960852	32.320701534606954	39.14813654870028	21.327904791731914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	3.0
16	1.5
17	0.0
18	1.5
19	3.0
20	13.5
21	41.5
22	58.0
23	67.5
24	106.5
25	195.0
26	305.0
27	324.0
28	278.0
29	246.5
30	224.0
31	181.0
32	153.0
33	149.0
34	140.0
35	142.0
36	147.5
37	150.0
38	139.5
39	162.5
40	164.5
41	165.5
42	179.0
43	166.5
44	162.5
45	161.5
46	161.5
47	167.0
48	192.5
49	157.0
50	80.0
51	62.5
52	56.0
53	37.0
54	29.5
55	19.5
56	14.5
57	22.5
58	21.0
59	10.5
60	6.5
61	6.0
62	4.0
63	1.0
64	0.5
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.36250000000000004
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.2671245945430262
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	4.0
22-23	4.0
24-25	11.0
26-27	12.0
28-29	17.0
30-31	12.0
32-33	15.0
34-35	14.0
36-37	24.0
38-39	18.0
40-41	26.0
42-43	23.0
44-45	37.0
46-47	39.0
48-49	45.0
50-51	94.0
52-53	54.0
54-55	42.0
56-57	59.0
58-59	46.0
60-61	104.0
62-63	104.0
64-65	116.0
66-67	86.0
68-69	124.0
70-71	108.0
72-73	66.0
74-75	55.0
76-77	75.0
78-79	65.0
80-81	73.0
82-83	0.0
84-85	51.0
86-87	70.0
88-89	55.0
90-91	109.0
92-93	48.0
94-95	92.0
96-97	99.0
98-99	186.0
100-101	1718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.98365122615804	15.775
2	19.20980926430518	14.099999999999998
3	12.534059945504087	13.8
4	8.174386920980927	12.0
5	6.4032697547683926	11.75
6	3.474114441416894	7.6499999999999995
7	2.3841961852861036	6.125
8	1.2942779291553135	3.8
9	1.021798365122616	3.375
>10	2.5204359673024523	11.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	23	0.575	No Hit
AAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	20	0.5	No Hit
TTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAAT	19	0.475	No Hit
AAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	19	0.475	No Hit
TACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTT	17	0.42500000000000004	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	16	0.4	No Hit
CCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	16	0.4	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	14	0.35000000000000003	No Hit
TGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAA	13	0.325	No Hit
GCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	13	0.325	No Hit
TAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	13	0.325	No Hit
GTGCGACTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCT	13	0.325	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	13	0.325	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	13	0.325	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	13	0.325	No Hit
AGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	12	0.3	No Hit
ACATTCTCCATAAAAGGGACGGGGTTTTGCCATTCGGGGTCTTCGCTCTG	12	0.3	No Hit
AGTTAACGCCCGGCTAAGACCTTGATTTTTAAGACCAACTTAGACTGATC	12	0.3	No Hit
CCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAA	11	0.27499999999999997	No Hit
AATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	11	0.27499999999999997	No Hit
AAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACC	11	0.27499999999999997	No Hit
TGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	11	0.27499999999999997	No Hit
GCCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGC	10	0.25	No Hit
CTGTCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	10	0.25	No Hit
AATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGA	10	0.25	No Hit
TAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	10	0.25	No Hit
ATACTGCAGCACCAGGCACATGTAGTACACAATGAACATAAAAATAATAA	10	0.25	No Hit
ATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAAT	10	0.25	No Hit
TCGGAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
TGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
TCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAA	10	0.25	No Hit
GATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	10	0.25	No Hit
GCTGGCAGTGGTGGGTAATACATGCCTTTCATCTCAGTAATTAGGAGGCG	10	0.25	No Hit
ATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGC	10	0.25	No Hit
CTGGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCT	10	0.25	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
TTGTGAAATTAAAGAACTGAAGCAGTCACGAGAGATGGCATTAGAAGACC	10	0.25	No Hit
GACATGGGGAAGAATCTAATATACTTAAGGCTGAATTAGACAATGTTACA	9	0.22499999999999998	No Hit
CGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACT	9	0.22499999999999998	No Hit
GCTCAGGGGTGGAGCTTCCTGCTCATTCGTTCTGCCACGCCCACTGCTAG	9	0.22499999999999998	No Hit
AATGTAGGCTGTAGCAAATAGTTTTACAAAACATATTTCACAATTAAGTT	9	0.22499999999999998	No Hit
ATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
AAGAAAATCAGCAAAGAATTGACCAGATGCTAGAATCTCATGCCTCAACT	9	0.22499999999999998	No Hit
TTGGAGGTGCAGCAAGAAAAGGATCAGAAGTTGTTAGAGATGGAAAAGAT	9	0.22499999999999998	No Hit
TGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGAATGCG	9	0.22499999999999998	No Hit
ACATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTT	9	0.22499999999999998	No Hit
AGATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCAC	9	0.22499999999999998	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
AAAGAACTGAAGCAGTCACGAGAGATGGCATTAGAAGACCTGAAAAAGCT	9	0.22499999999999998	No Hit
TCCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGGCACTTGATG	9	0.22499999999999998	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
TGGGTGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	9	0.22499999999999998	No Hit
TGTATAAAATTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTAT	8	0.2	No Hit
CTTGTCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTG	8	0.2	No Hit
AGAAATATTCCATTGACTGTGTGCTGTAACGTGGGAAAATGTCATGTTTT	8	0.2	No Hit
GCAAGGAGGCGGAGTCGAATGGGTCTCTAAGTTGGCCAGGACAACAGAGA	8	0.2	No Hit
GAGTTACCAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAA	8	0.2	No Hit
ACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATC	8	0.2	No Hit
TTTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGTGCATTTCA	8	0.2	No Hit
GCTAACAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAG	8	0.2	No Hit
CTGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGGTT	8	0.2	No Hit
GATCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAA	8	0.2	No Hit
ATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTC	8	0.2	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	8	0.2	No Hit
AGGATGGGGGCTCTCTTGTCTCCCAAACCTTGAAGGTGTTCTTTTATTGG	8	0.2	No Hit
AATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTT	8	0.2	No Hit
ATCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAA	8	0.2	No Hit
GATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAG	8	0.2	No Hit
GCAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCA	8	0.2	No Hit
TTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATT	8	0.2	No Hit
ATGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	8	0.2	No Hit
TGCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	7	0.17500000000000002	No Hit
AATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAG	7	0.17500000000000002	No Hit
GTGTAGGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCAT	7	0.17500000000000002	No Hit
AGAATACAGATGAAATATAGTTTGCCATTGAAGGTCTTAATAGTTATATA	7	0.17500000000000002	No Hit
ATAAACAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAG	7	0.17500000000000002	No Hit
TAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCC	7	0.17500000000000002	No Hit
TTTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATATTACTG	7	0.17500000000000002	No Hit
GCCCCGCCCCTCAATAAAGTTCTGTGACC	7	0.17500000000000002	No Hit
CTTGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	7	0.17500000000000002	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
ATGGTGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCAC	7	0.17500000000000002	No Hit
TTAAAGAACTGAAGCAGTCACGAGAGATGGCATTAGAAGACCTGAAAAAG	7	0.17500000000000002	No Hit
GGCAAAACTCTGAGATCGAGGCCAGCCTGGTCTATAGAGTGAGTTCCAGG	7	0.17500000000000002	No Hit
TGATTCCCTGGAAAGTGTGGGCTATTTTGTTGGTGATGTGGTGATAATGA	7	0.17500000000000002	No Hit
TGCTGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TCCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	7	0.17500000000000002	No Hit
TCTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTT	7	0.17500000000000002	No Hit
CCACGAGCAAGACAAAGAACACTTAATTCAGGAGCTTAATTTTGAAAAAA	7	0.17500000000000002	No Hit
CAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	7	0.17500000000000002	No Hit
GTCGGGCGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGT	7	0.17500000000000002	No Hit
TTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAA	7	0.17500000000000002	No Hit
TCTGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTT	7	0.17500000000000002	No Hit
GAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
GATCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	7	0.17500000000000002	No Hit
TTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGT	7	0.17500000000000002	No Hit
TGTTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	7	0.17500000000000002	No Hit
GTGGATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	7	0.17500000000000002	No Hit
ACGGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGC	7	0.17500000000000002	No Hit
ACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGTGGGT	7	0.17500000000000002	No Hit
ACTGGAGTCAAATAGCCAAAACGTAAGTATTAAGTTTAGAATGTAATTTA	7	0.17500000000000002	No Hit
TTGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAG	7	0.17500000000000002	No Hit
GTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TTCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	7	0.17500000000000002	No Hit
AATTCAGGAGCTTAATTTTGAAAAAAACAAAGCTGTTCAAACTGCACTAG	7	0.17500000000000002	No Hit
TCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTAT	7	0.17500000000000002	No Hit
TTCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	6	0.15	No Hit
GGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATT	6	0.15	No Hit
CAATACTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTG	6	0.15	No Hit
CTATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAG	6	0.15	No Hit
CAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAATGA	6	0.15	No Hit
GGGTAGGGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	6	0.15	No Hit
CATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACAATGTTACATC	6	0.15	No Hit
TTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTA	6	0.15	No Hit
CAATCGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCA	6	0.15	No Hit
TCGATGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCCTGAGTTCAAT	6	0.15	No Hit
GCCACGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGC	6	0.15	No Hit
GCTACGTACTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	6	0.15	No Hit
AAACAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCA	6	0.15	No Hit
CACACCAGGCACATGTAGTACACAATGAACATAAAAATAATAAAACCAAA	6	0.15	No Hit
TCGTCCTAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAA	6	0.15	No Hit
TGGAGGTTGAACTTGCACTAAAGGAAGCAGAAACAGATGAGATAAAGATC	6	0.15	No Hit
CAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGT	6	0.15	No Hit
TGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACC	6	0.15	No Hit
GCACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	6	0.15	No Hit
TGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATATACTTAAGG	6	0.15	No Hit
AGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
ATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGCC	6	0.15	No Hit
CAAAAACACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	6	0.15	No Hit
TAATGATTACTTCTTTTCTGTTGTATAGCTTTTCCCTATGGTAATTCCTG	6	0.15	No Hit
GACCTGGCCTCCATGTGTGCCTGCATCTGCCCCCACATCCCATTTCTAAAGTAAGAGGGAAACTTTTGTTAT	6	0.15	No Hit
AGGTCAGGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATAT	6	0.15	No Hit
TTTGGAGCGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAAT	6	0.15	No Hit
GTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	6	0.15	No Hit
GCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGT	6	0.15	No Hit
TAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTA	6	0.15	No Hit
TCTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGT	6	0.15	No Hit
TGGCTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATT	6	0.15	No Hit
TGGGATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTT	6	0.15	No Hit
TGTGAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	6	0.15	No Hit
CAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	6	0.15	No Hit
GGAAGCCAGACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGC	6	0.15	No Hit
GATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTCACTTTAAAG	6	0.15	No Hit
GGGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAATGAAGC	6	0.15	No Hit
TTACATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	6	0.15	No Hit
GGAAAATGGCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATAT	6	0.15	No Hit
CGTCTGCCCCCACATCCCATTTCTAAAGTAAGAGGGAAACTTTTGTTATGATCT	6	0.15	No Hit
CATGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	6	0.15	No Hit
GGTCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATAT	6	0.15	No Hit
GGGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	6	0.15	No Hit
TTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	6	0.15	No Hit
TCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGG	6	0.15	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
ATTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCTT	6	0.15	No Hit
CTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	6	0.15	No Hit
CAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCA	5	0.125	No Hit
AAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCAC	5	0.125	No Hit
GAGACGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGG	5	0.125	No Hit
TGATTACTTCTTTTCTGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGA	5	0.125	No Hit
CTCTGAAAATGAATATAATTAAAGGATTCTCACTCC	5	0.125	No Hit
GATAACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	5	0.125	No Hit
GCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAG	5	0.125	No Hit
TTGTGCCCGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTC	5	0.125	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	5	0.125	No Hit
CAGACGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACA	5	0.125	No Hit
CCTTGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	5	0.125	No Hit
GTTGTTTTGCGATGCTTCATGCTGATCAAGATGGAGAAAAACTGCAGGCA	5	0.125	No Hit
TCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAGGAATT	5	0.125	No Hit
AATCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAG	5	0.125	No Hit
CCACCCCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAA	5	0.125	No Hit
GTTAGATCACAGGAAGACTGGCATACCTATTAGATCATCTAAAATAAAGT	5	0.125	No Hit
CGGGCAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGTGG	5	0.125	No Hit
GTGTTTGGTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	5	0.125	No Hit
CAATCGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGC	5	0.125	No Hit
ACTGTCCTGAGTTCAAATCCCAGCAACCACATGGTAGATCACAACCATCC	5	0.125	No Hit
ATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
CGCCACACACACACAAATTACTTTTAAATATACTTTTCGAAAGAACACAC	5	0.125	No Hit
CGGTAATGGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
AAGTTGTTAGAGATGGAAAAGATAATGCATACTCAACATTGTGAAATTAA	5	0.125	No Hit
TGCAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCA	5	0.125	No Hit
TCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTG	5	0.125	No Hit
AGTCAGGTGCTCTCACCCACCGAGCCATCTCACCAGTCCCAGACCTCTTT	5	0.125	No Hit
GCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTG	5	0.125	No Hit
AAAGCCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCT	5	0.125	No Hit
GGATGTGTTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGT	5	0.125	No Hit
GGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCA	5	0.125	No Hit
TTACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	5	0.125	No Hit
AATGAAGCCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTA	5	0.125	No Hit
CTCAGCACCCATATGGCAGCTCACAACCATCTGAATCTTCAATTTCAGGG	5	0.125	No Hit
TTCGGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGA	5	0.125	No Hit
CCATATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCT	5	0.125	No Hit
AAGTATTGGCTAAGGTGATGCTCATTGCGAGTACCTTTTGTCTATGGTTA	5	0.125	No Hit
TGAGAAAGAGTTATTAGAAAAAGTTAAACATCTTGAGAATCAAATAGCCA	5	0.125	No Hit
AAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTC	5	0.125	No Hit
CTCTTCGCCTGCTTGCCGTGTGAGACTGAGTAACTGCTAGATCCTTGGAC	5	0.125	No Hit
GGTCTGCCGGGCGTGGTGGCCCACACCTTTAATCCCAGAACTCGGGAGGC	5	0.125	No Hit
TATGGCCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
GTTGACGGACCACAATATGTCTTTGGAGAAATTAAAAAAAGAAAATCAGC	5	0.125	No Hit
GTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTC	5	0.125	No Hit
CAGAGGCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGA	5	0.125	No Hit
GGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	5	0.125	No Hit
GTCTAGGGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCGGCAAGA	5	0.125	No Hit
ACTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGTA	5	0.125	No Hit
CAAACTCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	5	0.125	No Hit
GCTCCTGTACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	5	0.125	No Hit
TCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAAT	5	0.125	No Hit
TTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAA	5	0.125	No Hit
GAACCCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCC	5	0.125	No Hit
CCAGACAGCCAGAGCTATAAAGAGAAAAGCTGTCTCGAAAAACC	5	0.125	No Hit
GCATTGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGA	5	0.125	No Hit
CATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAGGAATTA	5	0.125	No Hit
CGAATGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	5	0.125	No Hit
CCTGATGACTTGAGCACTGTGGAGAGTGAATCCAGGATGCTCTGAGGCTT	5	0.125	No Hit
GTCTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGA	5	0.125	No Hit
TTTGAGAATCAAATAGCCAAAACGTAAGTATTAAGTTTAGAATGTAATTT	5	0.125	No Hit
TGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGAC	5	0.125	No Hit
TCGACGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAG	5	0.125	No Hit
GCGGTGGGTATTAAAGCTTTGGGTCTGTTACC	5	0.125	No Hit
CTGGGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	5	0.125	No Hit
ATGCTACATGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCG	5	0.125	No Hit
GTAGAGTCACTGTTTTAATTTCTTCCATGTTATATTTCCAAGATAAAAAA	5	0.125	No Hit
CATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATT	5	0.125	No Hit
CCTTTGCTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTG	5	0.125	No Hit
CATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
CATGAATTCACTTCGATTAAACATGAAAAGGATGCTATTGTCTGTGTGCA	5	0.125	No Hit
TCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
TTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
CGGACGAATGGGTCTCTAAGTTGGCCAGGACAACAGAGAAACCCTGTCTCAAAAAAAAAAGAACAACAACAACAT	5	0.125	No Hit
TTTGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTT	5	0.125	No Hit
CGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAA	5	0.125	No Hit
TAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
GCTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GCAATCAAAACTCTTAAAATACAAAGGGATACTTTTTTCTTATTTGTACC	5	0.125	No Hit
AGTTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	5	0.125	No Hit
AAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCT	5	0.125	No Hit
TATGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TCTCGAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	5	0.125	No Hit
TGCGATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAG	5	0.125	No Hit
CGTCAGCTCATGTCAGCAGGTAGGTGTCTCTGGAAGCAGTGAGTAGAGTC	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
ATACGGCCATAAATACCTTAGGAGAGTCAGATTAACTGTTGGTTCTGAGC	5	0.125	No Hit
CATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
GTTTTCAATTTCTTCCATGTTATATTTCCAAGATAAAAAATTTGGATGTT	5	0.125	No Hit
TGCTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGAAAA	5	0.125	No Hit
GAGCTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACC	5	0.125	No Hit
TTTGCTGAGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAG	5	0.125	No Hit
CGTCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GTTCAAAACCTAGCAACCACATGGTGGCTCGCAACCATCTGTAATGGGAT	5	0.125	No Hit
GGAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810779 spots for SRR25194573.sra
Written 810779 spots for SRR25194573.sra
Read 810790 spots for SRR25194573.sra
Written 810790 spots for SRR25194573.sra
SRR ids: ['SRR25194573.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_j9plsdwx
SRR25194573.sra spots: 12972475
blocks: [[1, 810779], [810780, 1621558], [1621559, 2432337], [2432338, 3243116], [3243117, 4053895], [4053896, 4864674], [4864675, 5675453], [5675454, 6486232], [6486233, 7297011], [7297012, 8107790], [8107791, 8918569], [8918570, 9729348], [9729349, 10540127], [10540128, 11350906], [11350907, 12161685], [12161686, 12972475]]
SRR25194573 file size 286712
SRR25194573 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25194573 SRR25194573_1.fastq
Input file:	SRR25194573_1.fastq
trimmed:	SRR25194573-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Sun Jun 21 08:17:34 2026 >> started

Sun Jun 21 08:17:43 2026 >> done (8.308s)
12972475 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
12972475 (100.00%) reads available; of these:
      77 ( 0.00%) trimmed reads available after processing
12972398 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    9867	  0.08%
 21	    9835	  0.08%
 22	    9590	  0.07%
 23	   10066	  0.08%
 24	   10515	  0.08%
 25	   11330	  0.09%
 26	   11992	  0.09%
 27	   13256	  0.10%
 28	   15205	  0.12%
 29	   17660	  0.14%
 30	   19399	  0.15%
 31	   23020	  0.18%
 32	   26056	  0.20%
 33	   27012	  0.21%
 34	   27257	  0.21%
 35	   27711	  0.21%
 36	   29462	  0.23%
 37	   30825	  0.24%
 38	   33427	  0.26%
 39	   36288	  0.28%
 40	   39391	  0.30%
 41	   40463	  0.31%
 42	   42847	  0.33%
 43	   46306	  0.36%
 44	   48853	  0.38%
 45	   47549	  0.37%
 46	   48548	  0.37%
 47	   50478	  0.39%
 48	   51926	  0.40%
 49	   54320	  0.42%
 50	   56053	  0.43%
 51	   57927	  0.45%
 52	   63843	  0.49%
 53	   66976	  0.52%
 54	   68872	  0.53%
 55	   74353	  0.57%
 56	   88873	  0.69%
 57	   91946	  0.71%
 58	   91007	  0.70%
 59	   83528	  0.64%
 60	   82239	  0.63%
 61	   82616	  0.64%
 62	   84689	  0.65%
 63	   86274	  0.67%
 64	   85293	  0.66%
 65	   86434	  0.67%
 66	   90625	  0.70%
 67	   92153	  0.71%
 68	   93205	  0.72%
 69	   98375	  0.76%
 70	  100104	  0.77%
 71	  103999	  0.80%
 72	  104639	  0.81%
 73	   99094	  0.76%
 74	   99111	  0.76%
 75	   99865	  0.77%
 76	   99448	  0.77%
 77	  101743	  0.78%
 78	  106936	  0.82%
 79	  118230	  0.91%
 80	  147658	  1.14%
 81	  175042	  1.35%
 82	     828	  0.01%
 83	   20969	  0.16%
 84	   79236	  0.61%
 85	   94812	  0.73%
 86	   97484	  0.75%
 87	  101037	  0.78%
 88	  102079	  0.79%
 89	  102044	  0.79%
 90	   99965	  0.77%
 91	  111696	  0.86%
 92	   67730	  0.52%
 93	   71792	  0.55%
 94	   96894	  0.75%
 95	  121585	  0.94%
 96	  148128	  1.14%
 97	  241553	  1.86%
 98	  383554	  2.96%
 99	  234549	  1.81%
100	 1024514	  7.90%
101	 5920422	 45.64%
12972475 reads passed initial QC


criterion=sequence-density
sequence-density=0.58
sequence-density-rank=1
fanout-score=3.96
fanout-score-rank=35
prefix-density=1.75
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=698.99
fanout-score-rank=1
prefix-density=6.19
prefix-fanout=1.0
sequence=CATGATACTGAGTTGGTACTTTTTTTGGTCCCTATTTCACAAGGGTTAGGATAGATTAACCAAGAAAGGACAAGTGACAGACTGAAAGGGGCTGGAAAAACAAAGAGGAAAAGGCCTGTCACTTCTGTATAGTTGATGGTTCCTGTCACAAGCCCAGGTCACAAACAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATGTTAACAAAGGAAGGTGGATAAAATACAGTTTCTACTGCCTAAAGAATTTTGGCTCTATTAAAATGTAAGTGTGTGGCT
                                 Started job on |	Jun 21 08:17:59
                             Started mapping on |	Jun 21 08:18:00
                                    Finished on |	Jun 21 08:18:23
       Mapping speed, Million of reads per hour |	2030.47

                          Number of input reads |	12972475
                      Average input read length |	87
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11075479
                        Uniquely mapped reads % |	85.38%
                          Average mapped length |	86.61
                       Number of splices: Total |	1070930
            Number of splices: Annotated (sjdb) |	1060279
                       Number of splices: GT/AG |	1063526
                       Number of splices: GC/AG |	3925
                       Number of splices: AT/AC |	425
               Number of splices: Non-canonical |	3054
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.48
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1777451
             % of reads mapped to multiple loci |	13.70%
        Number of reads mapped to too many loci |	61632
             % of reads mapped to too many loci |	0.48%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.44%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	119545	119545	119545
N_multimapping	1777451	1777451	1777451
N_noFeature	919876	958998	10012273
N_ambiguous	1142729	119069	699594
UnstrandedReadsAssigned:9012874 PositiveStrandReadsAssigned:9997412 NegativeStrandReadsAssigned:363612
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=79 echo kmer=75
SRR25194573 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194573-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 12,972,475 reads, 8,005,238 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,040 rounds

 109283 SRR25194573.ke.tsv
  52636 SRR25194573.se.tsv
 161919 total
==> SRR25194573.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194573.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194573 completed mapping pipeline successfully
