Starting /dee2/code/volunteer_pipeline.sh SRR25194571
    current disk space = 1251599232
    free memory = 200879596 
SRR25194571 SRAfilesize
b15192007f8a7460ce15b36114df1c0f  SRR25194571.sra
SRR25194571.sra file validated
SRR25194571 is single end
SRR25194571 is conventional basespace
SRR25194571 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2615	34.0	34.0	34.0	34.0	34.0
2	33.437	34.0	34.0	34.0	34.0	34.0
3	33.348	34.0	34.0	34.0	34.0	34.0
4	33.382	34.0	34.0	34.0	34.0	34.0
5	33.5345	34.0	34.0	34.0	34.0	34.0
6	33.6155	34.0	34.0	34.0	34.0	34.0
7	33.583	34.0	34.0	34.0	34.0	34.0
8	33.555	34.0	34.0	34.0	34.0	34.0
9	33.638	34.0	34.0	34.0	34.0	34.0
10-11	33.61750000000001	34.0	34.0	34.0	34.0	34.0
12-13	33.632999999999996	34.0	34.0	34.0	34.0	34.0
14-15	33.594750000000005	34.0	34.0	34.0	34.0	34.0
16-17	33.6065	34.0	34.0	34.0	34.0	34.0
18-19	33.49925	34.0	34.0	34.0	34.0	34.0
20-21	33.597687155733595	34.0	34.0	34.0	34.0	34.0
22-23	33.60511545495898	34.0	34.0	34.0	34.0	34.0
24-25	33.58476703838324	34.0	34.0	34.0	34.0	34.0
26-27	33.62165565324582	34.0	34.0	34.0	34.0	34.0
28-29	33.66351111655777	34.0	34.0	34.0	34.0	34.0
30-31	33.62031414291573	34.0	34.0	34.0	34.0	34.0
32-33	33.5881558265068	34.0	34.0	34.0	34.0	34.0
34-35	33.62249632673837	34.0	34.0	34.0	34.0	34.0
36-37	33.62403395060653	34.0	34.0	34.0	34.0	34.0
38-39	33.571924471143994	34.0	34.0	34.0	34.0	34.0
40-41	33.5910052808502	34.0	34.0	34.0	34.0	34.0
42-43	33.503231879908085	34.0	34.0	34.0	34.0	34.0
44-45	33.488662163429964	34.0	34.0	34.0	34.0	34.0
46-47	33.515111661613815	34.0	34.0	34.0	34.0	34.0
48-49	33.47533948165434	34.0	34.0	34.0	34.0	34.0
50-51	33.52362555743694	34.0	34.0	34.0	34.0	34.0
52-53	33.49316349666104	34.0	34.0	34.0	34.0	34.0
54-55	33.49696950794107	34.0	34.0	34.0	34.0	34.0
56-57	33.45964668472301	34.0	34.0	34.0	34.0	34.0
58-59	33.38614596914974	34.0	34.0	34.0	34.0	34.0
60-61	33.440003136282186	34.0	34.0	34.0	34.0	34.0
62-63	33.320087889872106	34.0	34.0	34.0	34.0	34.0
64-65	33.35563253808058	34.0	34.0	34.0	34.0	34.0
66-67	33.32240704570035	34.0	34.0	34.0	34.0	34.0
68-69	33.27445900682538	34.0	34.0	34.0	34.0	34.0
70-71	33.21710334567727	34.0	34.0	34.0	34.0	34.0
72-73	33.19963263758909	34.0	34.0	34.0	34.0	34.0
74-75	33.17089807461231	34.0	34.0	34.0	34.0	34.0
76-77	33.0029556603498	34.0	34.0	34.0	34.0	34.0
78-79	33.13376981664227	34.0	34.0	34.0	34.0	34.0
80-81	33.023698801108885	34.0	34.0	34.0	34.0	34.0
82-83	33.030354131534565	34.0	34.0	34.0	34.0	34.0
84-85	33.144395011636846	34.0	34.0	34.0	34.0	34.0
86-87	32.96089453927141	34.0	34.0	34.0	34.0	34.0
88-89	32.98586433378805	34.0	34.0	34.0	34.0	34.0
90-91	33.09683742473368	34.0	34.0	34.0	34.0	34.0
92-93	33.000017968557785	34.0	34.0	34.0	34.0	34.0
94-95	33.01814054923631	34.0	34.0	34.0	34.0	34.0
96-97	33.00558169344308	34.0	34.0	34.0	34.0	34.0
98-99	32.90697462726611	34.0	34.0	34.0	34.0	34.0
100-101	32.58977173401786	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	2.0
26	14.0
27	22.0
28	32.0
29	51.0
30	54.0
31	87.0
32	255.0
33	2503.0
34	978.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.074999999999996	22.725	30.875000000000004	21.325
2	26.525	25.474999999999998	27.224999999999998	20.775
3	30.675	27.775	24.325	17.224999999999998
4	30.125	25.85	22.3	21.725
5	28.449999999999996	29.349999999999998	24.625	17.575
6	28.299999999999997	26.35	24.85	20.5
7	23.65	28.175	28.325	19.85
8	24.9	27.775	28.275	19.05
9	23.325000000000003	26.375	27.325	22.975
10-11	26.137500000000003	26.275	27.737499999999997	19.85
12-13	24.8125	30.875000000000004	28.199999999999996	16.1125
14-15	22.7125	31.5125	31.412499999999998	14.3625
16-17	22.575	30.225	32.4	14.799999999999999
18-19	20.740276035131743	28.983688833124216	33.03638644918444	17.2396486825596
20-21	19.427070302727046	31.098323742807104	32.21165874405804	17.262947210407805
22-23	21.13719091251412	31.027990460650184	31.20371532571859	16.631103301117108
24-25	20.596751856980987	31.549792269923206	30.95807629359184	16.895379579503967
26-27	19.43601416287304	31.727364693980782	31.929691451694488	16.906929691451694
28-29	20.761904761904763	31.33968253968254	30.36190476190476	17.536507936507935
30-31	20.6646294881589	31.130634071810544	30.150241914947795	18.05449452508276
32-33	22.288925788734193	32.62230169881211	28.036786307318945	17.051986205134757
34-35	19.95380469652252	31.707943025792378	30.232259720261773	18.105992557423328
36-37	20.160455486542443	31.262939958592135	30.913561076604555	17.66304347826087
38-39	20.858815875081326	32.30969420949902	29.355888093689003	17.475601821730642
40-41	20.984422044770255	31.797355674826548	29.20539337609635	18.012828904306847
42-43	19.24802110817942	36.93931398416886	27.598944591029024	16.21372031662269
44-45	18.741668888296452	34.1109037589976	30.685150626499603	16.462276726206344
46-47	20.02961367613407	33.26154260331135	29.142549468299904	17.56629425225468
48-49	18.836640391410707	34.860016308779564	29.410165805925526	16.893177493884206
50-51	17.945148266740915	34.010858972574134	32.60476124182097	15.439231518863986
52-53	17.039660056657226	36.303116147308785	31.770538243626063	14.886685552407933
54-55	21.128778112018335	31.98682137229623	31.643031084371863	15.241369431313565
56-57	19.197791018747274	33.861357360848714	32.974858305478854	13.965993314925157
58-59	19.30831493745401	32.68579838116262	35.526122148638706	12.479764532744666
60-61	18.582747794887126	29.89983555090447	36.956196740917925	14.561219913290477
62-63	19.26577201912695	31.081289526453805	36.140675613142065	13.512262841277186
64-65	18.692037096258396	29.341221618164376	36.52062679884874	15.446114486728494
66-67	19.159802306425043	30.0164744645799	34.69522240527183	16.12850082372323
68-69	17.314246762099522	30.981595092024538	35.906612133606	15.797546012269938
70-71	16.867897727272727	32.49289772727273	34.58806818181818	16.051136363636363
72-73	17.56732002198205	32.93643524455028	34.09049276424253	15.40575196922513
74-75	17.02525724976614	34.79887745556595	32.81571562207671	15.360149672591206
76-77	18.216904670011576	31.47433423388653	34.291779235816286	16.016981860285604
78-79	18.08066759388039	30.141068944963244	33.97575998410491	17.80250347705146
80-81	18.166972851602367	29.84282506634007	34.43559910185752	17.55460298020004
82-83	18.402192242833053	31.02866779089376	34.78077571669477	15.788364249578416
84-85	18.540644335395775	30.595263494772773	34.883720930232556	15.98037123959889
86-87	20.579837469800133	30.441467164506918	32.63782121678015	16.340874148912803
88-89	19.299033924960682	32.060211188496964	30.599865198831722	18.040889687710624
90-91	19.747899159663866	30.835667600373483	33.23996265172735	16.176470588235293
92-93	16.438684905207584	30.885529157667385	36.42908567314615	16.24670026397888
94-95	16.1779324055666	33.57355864811134	31.237574552683895	19.01093439363817
96-97	16.849629818738833	33.750319121776876	32.67806995149349	16.72198110799081
98-99	16.165207877461707	30.47045951859956	33.20568927789934	20.15864332603939
100-101	8.194701170671596	33.67221195317314	37.030191004313004	21.102895871842268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.5
18	5.0
19	11.0
20	15.5
21	25.5
22	42.0
23	71.0
24	96.0
25	152.0
26	229.5
27	247.0
28	225.0
29	200.0
30	189.5
31	172.0
32	159.5
33	183.5
34	184.0
35	170.0
36	157.0
37	145.5
38	149.5
39	174.5
40	195.5
41	198.5
42	193.0
43	194.0
44	196.5
45	197.5
46	204.0
47	202.5
48	174.5
49	117.0
50	86.0
51	69.0
52	53.5
53	56.0
54	59.0
55	47.5
56	35.5
57	32.0
58	29.0
59	18.5
60	12.5
61	7.5
62	2.0
63	0.5
64	1.0
65	1.0
66	0.5
67	0.0
68	0.0
69	1.5
70	3.5
71	2.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.375
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.23103581055063535
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	14.0
22-23	8.0
24-25	21.0
26-27	17.0
28-29	11.0
30-31	11.0
32-33	17.0
34-35	27.0
36-37	28.0
38-39	22.0
40-41	21.0
42-43	41.0
44-45	43.0
46-47	23.0
48-49	81.0
50-51	73.0
52-53	42.0
54-55	55.0
56-57	35.0
58-59	44.0
60-61	93.0
62-63	125.0
64-65	77.0
66-67	115.0
68-69	113.0
70-71	96.0
72-73	64.0
74-75	66.0
76-77	82.0
78-79	63.0
80-81	100.0
82-83	0.0
84-85	90.0
86-87	38.0
88-89	85.0
90-91	68.0
92-93	65.0
94-95	45.0
96-97	109.0
98-99	157.0
100-101	1715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.2482887367766	17.375
2	22.339763534536402	17.95
3	13.503422526446796	16.275000000000002
4	7.031736154324829	11.3
5	5.5382700684505295	11.125
6	3.2358431860609835	7.8
7	1.4312383322962041	4.025
8	1.4934660858742999	4.8
9	0.9956440572495333	3.5999999999999996
>10	1.1823273179838207	5.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATG	19	0.475	No Hit
AAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCT	14	0.35000000000000003	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	14	0.35000000000000003	No Hit
ATCCACACACATACACACACACAC	13	0.325	No Hit
AACGGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAAT	13	0.325	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	13	0.325	No Hit
CTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCA	13	0.325	No Hit
GTTGTGGAACTTCAAGAGAAACTTCAAGAAGAAAAAGCTAAGTTTCTGGAACAACTT	12	0.3	No Hit
GCTGCAGGAGTTGAAACTCAAAGTTTCTGACTTGTCAGACATGAGATGTA	12	0.3	No Hit
TTACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	11	0.27499999999999997	No Hit
CGTCTTTTACTGTGAACTTTTAAAGAATGCTTATGTTACATATGTTTATT	11	0.27499999999999997	No Hit
AATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	11	0.27499999999999997	No Hit
CCGATGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	11	0.27499999999999997	No Hit
CAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	11	0.27499999999999997	No Hit
GAGAGAAAAGAAAGAATGAGGAAATGCAAAATGTCAGAACCTCTTTGATT	11	0.27499999999999997	No Hit
AGCTAGAGAAGATTCTTCAAGCTTAGTTGCGGAACTTCAAGAGAAACTTC	11	0.27499999999999997	No Hit
GCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	10	0.25	No Hit
CTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
CCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAAATAAA	10	0.25	No Hit
GCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTG	9	0.22499999999999998	No Hit
ATGATGCGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGA	9	0.22499999999999998	No Hit
GAGACCGAAAACTTAAGAACAGAAATAAGTAAACTAAACCAAAAAATTCA	9	0.22499999999999998	No Hit
TACTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAATGATAC	9	0.22499999999999998	No Hit
TATACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	9	0.22499999999999998	No Hit
TTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAA	9	0.22499999999999998	No Hit
TTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATAT	9	0.22499999999999998	No Hit
ACTAAGGACCTGTACAATTTTAAGACAATAAAGAATTGAAAGTGT	9	0.22499999999999998	No Hit
TCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACT	9	0.22499999999999998	No Hit
AGATGTTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	9	0.22499999999999998	No Hit
TTGCCTGCATCTGGTCTCCATGTGTGCCTGCATCTGGCCTCCATGTGTGC	9	0.22499999999999998	No Hit
CAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGTGCATTTCAGAG	9	0.22499999999999998	No Hit
CATGCCTATAGAGGATCAGTTGACACAGATTATGCGGACTGGACAAAACA	9	0.22499999999999998	No Hit
CCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTT	9	0.22499999999999998	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
CGGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAA	9	0.22499999999999998	No Hit
TAGTTGCGGAACTTCAAGAGAAACTTCAAGAAGAAAAAGCTAAGTTTCTG	8	0.2	No Hit
TACTGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACT	8	0.2	No Hit
GGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	8	0.2	No Hit
AGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGT	8	0.2	No Hit
ACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGC	8	0.2	No Hit
CTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAA	8	0.2	No Hit
GTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAA	8	0.2	No Hit
TTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATA	8	0.2	No Hit
GATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGACAGC	8	0.2	No Hit
CATGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	8	0.2	No Hit
GTCCCGGTTGCTCTGCACCTTGCCTCCCATCCATATCCAATCCTTTTTCT	8	0.2	No Hit
TGGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAG	8	0.2	No Hit
CTTGGATTCCGCAATGGAGACAAGCATGATGTCTGTCCAAGAAAACATGT	8	0.2	No Hit
CGGCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	8	0.2	No Hit
TTTCATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	8	0.2	No Hit
CACTAAAGGAAAAGCATCAGCAAGAACTCCAATCTTTAAAAATTGAGTAT	8	0.2	No Hit
CAGCACAGGAGGCTGAGGCAGGAGCATTTGAGCAACAGAGAATGACAACA	8	0.2	No Hit
AATAGCCAAAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCT	8	0.2	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	8	0.2	No Hit
AACAGAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACA	8	0.2	No Hit
GGAGTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTGCGCCTTCGGTTTAATTTATTTTT	8	0.2	No Hit
AAGTGCATGATGAAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTA	8	0.2	No Hit
TGACCATGTGCTAGAATCTCATGCCTCAACTATTCAGGAAAAAGAGCAAC	8	0.2	No Hit
CTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	8	0.2	No Hit
TATGATCAAACTTGGCAGTGGTGGGTAATACATGCCTTTCATCTCAGTAA	7	0.17500000000000002	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAAT	7	0.17500000000000002	No Hit
AATAGCAGCAGGAGTTGAAACTCAAAGTTTCTGACTTGTCAGACATGAGA	7	0.17500000000000002	No Hit
TGGCCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCAC	7	0.17500000000000002	No Hit
GCGACGTACACTTAGAAACTTCATAAATGATGAGATGCAGGCCAAGGGGA	7	0.17500000000000002	No Hit
CTGGGCTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	7	0.17500000000000002	No Hit
AGGCTTAGAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCA	7	0.17500000000000002	No Hit
GGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAAT	7	0.17500000000000002	No Hit
CAAGAGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTG	7	0.17500000000000002	No Hit
AATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	7	0.17500000000000002	No Hit
CATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	7	0.17500000000000002	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
CACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGA	7	0.17500000000000002	No Hit
AGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	7	0.17500000000000002	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
CGAGAATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGAG	7	0.17500000000000002	No Hit
AACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGA	7	0.17500000000000002	No Hit
TTACCCCCTGGGTCCTTGGGAAAGTAATGGAAAAGGAATACTGTCAAGCC	7	0.17500000000000002	No Hit
TCAGGCAACTCGTTTCCTTGCAAGTGAAATTTGTAAACAACATACATCTC	7	0.17500000000000002	No Hit
CTGTGCGTTTACAAATCTGAAGAATTTGGGTACAGAAGGAGGAGGAGGAA	7	0.17500000000000002	No Hit
TACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGAAAA	7	0.17500000000000002	No Hit
GAGAGGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAG	7	0.17500000000000002	No Hit
CAGCACTTGGGAGGCAGAGACAGGTGAATTTCTGAGTTTGTGCCAGCCTG	7	0.17500000000000002	No Hit
AGGCGGTTCCACAAAGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAA	6	0.15	No Hit
CACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAATT	6	0.15	No Hit
ATGGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTA	6	0.15	No Hit
TTTGGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATA	6	0.15	No Hit
TCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAG	6	0.15	No Hit
CTGTAATTAGACAATGTTACATCTTTGCATCGCCAAGCATAT	6	0.15	No Hit
CAGATTTAAAGTTCCTTTGGGGACAAAGTTTTACAGAGTGAAAGCTGTGT	6	0.15	No Hit
AGCCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTG	6	0.15	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TCACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCAC	6	0.15	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
GATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	6	0.15	No Hit
CTTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CGAGTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	6	0.15	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
GTGAGTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	6	0.15	No Hit
TGACTGGATGTTTAAAGCTACCCTGAGCTACATAGTGAGTTCTAGGCCAG	6	0.15	No Hit
TTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
CAAAGCCTCGGATCCTTGGATCTCTTGCATCTGTGACTAACCACAGTGAT	6	0.15	No Hit
GTGGCTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAG	6	0.15	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGA	6	0.15	No Hit
TATGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACTAGCATATTACT	6	0.15	No Hit
GAGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGA	6	0.15	No Hit
CCGATAATAATGAGAGTTACCAGGTGGGTTTGTCAGAGTTAAGAGCTTTA	6	0.15	No Hit
CAGCCATCTCACCAGTCCCAGACCTCTTTTTAAAAGTTTTATTGAGGGGG	6	0.15	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	6	0.15	No Hit
GTCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	6	0.15	No Hit
TTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGT	6	0.15	No Hit
CGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAAC	6	0.15	No Hit
GGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGC	6	0.15	No Hit
GAAGATCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATT	6	0.15	No Hit
TTCTGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGT	6	0.15	No Hit
ACAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGC	6	0.15	No Hit
TTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
TGAGGTGTGGTTATATAATTAAATGTACTTAGATATACTGACAACAAAAC	6	0.15	No Hit
CCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	6	0.15	No Hit
TGTCAGCAGGTAGGTGTCTCTGGAAGCAGTGAGTAGAGTCACTGTTTTAA	6	0.15	No Hit
TGTATACCTCCAGGCTCAGTGGCCAGCCAGTTTAGCTGCATGGTAGCTCA	6	0.15	No Hit
TCACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGTGG	6	0.15	No Hit
CATGACCTTGGCAGTATTTAAATTGCATGTCTTGATACTCTTCAGTTATG	6	0.15	No Hit
AACAGCTGGCCATTGTTGGGTTTTTTTTTGTTGTTTGTTTGTTTGTTTGT	6	0.15	No Hit
ATGGCACACCGGACTCTGTGGGGCTCCCTCCATGTGCTGTCAGGAATCTC	6	0.15	No Hit
CGTTCGACGTACACTTAGAAACTTCATAAATGATGAGATGCAGGCCAAGG	6	0.15	No Hit
ACACAGAAAGCATTTCTAAGAGGGAAACTCATAGCTCTGAGTGCCTCCAA	6	0.15	No Hit
AATGGTACTCAAAACTGTAACTACAGTCCCACACGATGGTACTCAAAACT	6	0.15	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATC	6	0.15	No Hit
ATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGA	6	0.15	No Hit
TTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	6	0.15	No Hit
GAAGTAAGAATGATAATGGCACACCGGACTCTGTGGGGCTCCCTCCATGT	6	0.15	No Hit
GCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTG	6	0.15	No Hit
GGGCAGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCG	6	0.15	No Hit
AGTGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAA	5	0.125	No Hit
CTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCG	5	0.125	No Hit
CACGGGGATATAATTCCCCCCTTTTTTGTTTTTTAAGAAGATATTGTTTT	5	0.125	No Hit
GCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAG	5	0.125	No Hit
GCTTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	5	0.125	No Hit
ATTCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
ATCGTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	5	0.125	No Hit
CGATATGGGTTGGTCTTTGTTGTTCACCTGACAGTCTGTCTTAAATGCCT	5	0.125	No Hit
GCTCACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
CTGGAGATTTTCAGATAGAACTTTCTTAGAATAACTTTAAAAAGAGCACTTAACATAAATTT	5	0.125	No Hit
CAATCAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGA	5	0.125	No Hit
GCTCCCCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAA	5	0.125	No Hit
TCGGATGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGT	5	0.125	No Hit
ACGGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTT	5	0.125	No Hit
GTCAATCAGGGAGTGACACGTCCGAGGCGAAGGAGAATGCTCCTTAAGAG	5	0.125	No Hit
TTGATGGCACACCGGACTCTGTGGGGCTCCCTCCATGTGCTGTCAGGAAT	5	0.125	No Hit
GGCGCATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
AAAAGGCAACATAACAATATACTTCATTGGTGGACTGCACTTACCTTTTA	5	0.125	No Hit
GCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
ATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTG	5	0.125	No Hit
CTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAGATTTATTT	5	0.125	No Hit
CCAGTCACGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAA	5	0.125	No Hit
GCCGTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAA	5	0.125	No Hit
TTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAG	5	0.125	No Hit
TGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAAC	5	0.125	No Hit
CTGCCTTTAACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	5	0.125	No Hit
GCTGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTG	5	0.125	No Hit
ACTATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAA	5	0.125	No Hit
ATGGCCACCGGACTCTGTGGGGCTCCCTCCATGTGCTGTCAGGAATCTCC	5	0.125	No Hit
TGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTG	5	0.125	No Hit
AGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGT	5	0.125	No Hit
CGTGGGGAGCACAGTCTAGCATTGTACCCTGTTTTAAACTTAGTAGTCAG	5	0.125	No Hit
GATGCTGCCTGTATTAGAGAAATATTACTGTTATTTATATTCTTTATGAT	5	0.125	No Hit
GGGGTAAGGGTGTTTGAATGTCTTCATAAAAGGAGTTCGTAT	5	0.125	No Hit
AAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCT	5	0.125	No Hit
AGTTAAACATCTTGAGAATCAAATAGCCAAAACGTAAGTATTAAGTTTAG	5	0.125	No Hit
TTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAA	5	0.125	No Hit
GCGGAGTCGAATGGGTCTCTAAGTTGGCCAGGACAACAGAGAAACCCTGT	5	0.125	No Hit
AGGGCGCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAATTAATAC	5	0.125	No Hit
GACAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	5	0.125	No Hit
AGCGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TCAGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	5	0.125	No Hit
GCAGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTC	5	0.125	No Hit
ATCTGATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAA	5	0.125	No Hit
GTTGGTTCTGAGCAGTTCTTTGAGAGGGAACTCATTTATTCCTATCTCCAGTTTTAGCACTAAATGAAT	5	0.125	No Hit
GAGATGTTGCACAAGACTCTCACAGCAGTATACAAACCATCAAGGACGAT	5	0.125	No Hit
AAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACAG	5	0.125	No Hit
CTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTA	5	0.125	No Hit
CAAGGTGGGAGATTTGGTTCTCATCATCCTAGATGAGCGGCACGACAATT	5	0.125	No Hit
TTCCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
TCCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
TTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATC	5	0.125	No Hit
TTGATCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCT	5	0.125	No Hit
CTTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	5	0.125	No Hit
GGGAAAAAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAAC	5	0.125	No Hit
TCGTCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	5	0.125	No Hit
TTGCAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCC	5	0.125	No Hit
TAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAA	5	0.125	No Hit
TATCTGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAA	5	0.125	No Hit
ATAGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCT	5	0.125	No Hit
AAAGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGA	5	0.125	No Hit
CTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GTACATCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAA	5	0.125	No Hit
GAAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCT	5	0.125	No Hit
GGACAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTG	5	0.125	No Hit
TTATTGTCCTCTGAAAATGAATATAATTAAAGGATTCTCACTCC	5	0.125	No Hit
AGGGATTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCTTTATA	5	0.125	No Hit
CATGGTTGGGGGCATATTGGTCATGCCTATAGAGGATCAGTTGACACAGA	5	0.125	No Hit
AATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	5	0.125	No Hit
CAAAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACTAGCA	5	0.125	No Hit
TTTGCGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAAT	5	0.125	No Hit
CTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	5	0.125	No Hit
GGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTAT	5	0.125	No Hit
TGCTGGAGCCCATGCCGTCTTTGTGTACTGTGTGTACTGTCTAACTTAGC	5	0.125	No Hit
TGTGTTTTCTTAGATATTAGTTCTGCAAGCTGCCCTGGGTTTTAGCTATT	5	0.125	No Hit
ACAGACATCTGTAACAAGATCTGACGCCCTCTTCTGAAGTGTCTGAAGAC	5	0.125	No Hit
ACCAGCCTAGTAAGAATGATAATGGCACACCGGACTCTGTGGGGCTCCCT	5	0.125	No Hit
GCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
ACACGTACACTTAGAAACTTCATAAATGATGAGATGCAGGCCAAGGGGAT	5	0.125	No Hit
GGGGGGCATATTGGTCATGCCTATAGAGGATCAGTTGACACAGATTATGCGGACTGGACAAAACACTT	5	0.125	No Hit
AACTGAACAAAGATATTGAAGTAGCTAGGCTAGTTGATTGGAAAGAGTTT	5	0.125	No Hit
TTTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGT	5	0.125	No Hit
AAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	5	0.125	No Hit
TGTGGCCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAG	5	0.125	No Hit
AGGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAAT	5	0.125	No Hit
TGGTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTT	5	0.125	No Hit
TGGTAGCAGTGTTGTGGGGGAGCACAGTCTAGCATTGTACCCTGTTTTAA	5	0.125	No Hit
CTGGCACTTGATGTTACCATATTTATTTTAAGAGAGCACTAAAATATAATAAAGTAATGAGCTGGCTTTCTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
Read 1610118 spots for SRR25194571.sra
Written 1610118 spots for SRR25194571.sra
SRR ids: ['SRR25194571.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ujrcexqt
SRR25194571.sra spots: 12880944
blocks: [[1, 1610118], [1610119, 3220236], [3220237, 4830354], [4830355, 6440472], [6440473, 8050590], [8050591, 9660708], [9660709, 11270826], [11270827, 12880944]]
SRR25194571 file size 2749936
SRR25194571 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25194571 SRR25194571_1.fastq
Input file:	SRR25194571_1.fastq
trimmed:	SRR25194571-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Jun 21 06:43:08 2026 >> started

Sun Jun 21 06:43:13 2026 >> done (4.544s)
12880944 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
12880944 (100.00%) reads available; of these:
      71 ( 0.00%) trimmed reads available after processing
12880873 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	   11826	  0.09%
 21	   10941	  0.08%
 22	   10580	  0.08%
 23	   10851	  0.08%
 24	   11415	  0.09%
 25	   12466	  0.10%
 26	   12927	  0.10%
 27	   14722	  0.11%
 28	   16508	  0.13%
 29	   19026	  0.15%
 30	   21443	  0.17%
 31	   24967	  0.19%
 32	   27993	  0.22%
 33	   28743	  0.22%
 34	   28882	  0.22%
 35	   29417	  0.23%
 36	   31064	  0.24%
 37	   32851	  0.26%
 38	   35235	  0.27%
 39	   38266	  0.30%
 40	   40611	  0.32%
 41	   41916	  0.33%
 42	   45231	  0.35%
 43	   50067	  0.39%
 44	   51636	  0.40%
 45	   49809	  0.39%
 46	   48782	  0.38%
 47	   49964	  0.39%
 48	   51097	  0.40%
 49	   54349	  0.42%
 50	   56991	  0.44%
 51	   59066	  0.46%
 52	   65402	  0.51%
 53	   68612	  0.53%
 54	   69778	  0.54%
 55	   77996	  0.61%
 56	   96982	  0.75%
 57	   97882	  0.76%
 58	   96242	  0.75%
 59	   85185	  0.66%
 60	   82832	  0.64%
 61	   83660	  0.65%
 62	   83697	  0.65%
 63	   84698	  0.66%
 64	   86109	  0.67%
 65	   85749	  0.67%
 66	   91075	  0.71%
 67	   93276	  0.72%
 68	   94817	  0.74%
 69	   98168	  0.76%
 70	  101406	  0.79%
 71	  105222	  0.82%
 72	  105140	  0.82%
 73	   98204	  0.76%
 74	   96282	  0.75%
 75	   96471	  0.75%
 76	   95904	  0.74%
 77	   98342	  0.76%
 78	  102571	  0.80%
 79	  112864	  0.88%
 80	  140151	  1.09%
 81	  170454	  1.32%
 82	     818	  0.01%
 83	   20249	  0.16%
 84	   77211	  0.60%
 85	   92765	  0.72%
 86	   94709	  0.74%
 87	   97574	  0.76%
 88	   97749	  0.76%
 89	   98833	  0.77%
 90	   97031	  0.75%
 91	  104861	  0.81%
 92	   66103	  0.51%
 93	   70402	  0.55%
 94	   91783	  0.71%
 95	  119004	  0.92%
 96	  142137	  1.10%
 97	  231455	  1.80%
 98	  375616	  2.92%
 99	  241429	  1.87%
100	 1024101	  7.95%
101	 5842301	 45.36%
12880944 reads passed initial QC


criterion=sequence-density
sequence-density=0.60
sequence-density-rank=1
fanout-score=3.89
fanout-score-rank=37
prefix-density=1.78
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGGAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=46
fanout-score=44.64
fanout-score-rank=1
prefix-density=0.22
prefix-fanout=1.0
sequence=ATCCACCAAGTCCTGGCCCCTGGCTGCCTGGACAACTTCCCCCTGCTCTCTGCCTATGTGGCTCGCCTCAGTGCCCGGCCCAAGATCAAGGCCTTTCTGTCCTCCCCGGAACATGTGAACCGTCCCATCAATGGCAATGGCAAACAGTAGTGGACTGAAGAGACAAGAGCTTCTTGTCCCCGTTTTCCCAGCACTAATAAAGTTTGTAAGACAGAA
                                 Started job on |	Jun 21 06:43:18
                             Started mapping on |	Jun 21 06:43:18
                                    Finished on |	Jun 21 06:43:29
       Mapping speed, Million of reads per hour |	4215.58

                          Number of input reads |	12880944
                      Average input read length |	87
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11020003
                        Uniquely mapped reads % |	85.55%
                          Average mapped length |	86.35
                       Number of splices: Total |	1118938
            Number of splices: Annotated (sjdb) |	1108927
                       Number of splices: GT/AG |	1112573
                       Number of splices: GC/AG |	3518
                       Number of splices: AT/AC |	302
               Number of splices: Non-canonical |	2545
                      Mismatch rate per base, % |	0.47%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.47
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.23
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1737773
             % of reads mapped to multiple loci |	13.49%
        Number of reads mapped to too many loci |	49981
             % of reads mapped to too many loci |	0.39%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.56%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	123168	123168	123168
N_multimapping	1737773	1737773	1737773
N_noFeature	758498	790535	9884658
N_ambiguous	1224979	122425	817697
UnstrandedReadsAssigned:9036526 PositiveStrandReadsAssigned:10107043 NegativeStrandReadsAssigned:317648
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=78 echo kmer=73
SRR25194571 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194571-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 12,880,944 reads, 7,901,176 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,048 rounds

 109283 SRR25194571.ke.tsv
  52636 SRR25194571.se.tsv
 161919 total
==> SRR25194571.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194571.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194571 completed mapping pipeline successfully
