Starting /dee2/code/volunteer_pipeline.sh SRR25194570
    current disk space = 1241366724
    free memory = 200122336 
SRR25194570 SRAfilesize
1c4224a9532370c58843435e117b46a5  SRR25194570.sra
SRR25194570.sra file validated
SRR25194570 is single end
SRR25194570 is conventional basespace
SRR25194570 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.205	34.0	34.0	34.0	34.0	34.0
2	33.4435	34.0	34.0	34.0	34.0	34.0
3	33.4015	34.0	34.0	34.0	34.0	34.0
4	33.378	34.0	34.0	34.0	34.0	34.0
5	33.524	34.0	34.0	34.0	34.0	34.0
6	33.575	34.0	34.0	34.0	34.0	34.0
7	33.522	34.0	34.0	34.0	34.0	34.0
8	33.5595	34.0	34.0	34.0	34.0	34.0
9	33.5325	34.0	34.0	34.0	34.0	34.0
10-11	33.59325	34.0	34.0	34.0	34.0	34.0
12-13	33.5845	34.0	34.0	34.0	34.0	34.0
14-15	33.6395	34.0	34.0	34.0	34.0	34.0
16-17	33.582499999999996	34.0	34.0	34.0	34.0	34.0
18-19	33.46275	34.0	34.0	34.0	34.0	34.0
20-21	33.54501776776777	34.0	34.0	34.0	34.0	34.0
22-23	33.53380835664524	34.0	34.0	34.0	34.0	34.0
24-25	33.53044669650329	34.0	34.0	34.0	34.0	34.0
26-27	33.59145802422725	34.0	34.0	34.0	34.0	34.0
28-29	33.57857224658753	34.0	34.0	34.0	34.0	34.0
30-31	33.577385676314286	34.0	34.0	34.0	34.0	34.0
32-33	33.59817843026421	34.0	34.0	34.0	34.0	34.0
34-35	33.53381696842591	34.0	34.0	34.0	34.0	34.0
36-37	33.57797082505972	34.0	34.0	34.0	34.0	34.0
38-39	33.53341851328429	34.0	34.0	34.0	34.0	34.0
40-41	33.57545617327926	34.0	34.0	34.0	34.0	34.0
42-43	33.53312533736724	34.0	34.0	34.0	34.0	34.0
44-45	33.52237476718928	34.0	34.0	34.0	34.0	34.0
46-47	33.56343963289854	34.0	34.0	34.0	34.0	34.0
48-49	33.53955469088817	34.0	34.0	34.0	34.0	34.0
50-51	33.51823548729155	34.0	34.0	34.0	34.0	34.0
52-53	33.496259655276724	34.0	34.0	34.0	34.0	34.0
54-55	33.41359048560136	34.0	34.0	34.0	34.0	34.0
56-57	33.50056061309435	34.0	34.0	34.0	34.0	34.0
58-59	33.436763301164454	34.0	34.0	34.0	34.0	34.0
60-61	33.49247868973674	34.0	34.0	34.0	34.0	34.0
62-63	33.35649671923631	34.0	34.0	34.0	34.0	34.0
64-65	33.348324859646254	34.0	34.0	34.0	34.0	34.0
66-67	33.28835063508741	34.0	34.0	34.0	34.0	34.0
68-69	33.18782608115501	34.0	34.0	34.0	34.0	34.0
70-71	33.12802067338733	34.0	34.0	34.0	34.0	34.0
72-73	33.17879988375117	34.0	34.0	34.0	34.0	34.0
74-75	33.1516398186983	34.0	34.0	34.0	34.0	34.0
76-77	33.031518739222264	34.0	34.0	34.0	34.0	34.0
78-79	33.23881151238111	34.0	34.0	34.0	34.0	34.0
80-81	33.18849509762724	34.0	34.0	34.0	34.0	34.0
82-83	33.15208333333334	34.0	34.0	34.0	34.0	34.0
84-85	33.25364029985623	34.0	34.0	34.0	34.0	34.0
86-87	33.087907569302715	34.0	34.0	34.0	34.0	34.0
88-89	33.00061200828527	34.0	34.0	34.0	34.0	34.0
90-91	33.01504900568151	34.0	34.0	34.0	34.0	34.0
92-93	32.96597503397453	34.0	34.0	34.0	34.0	34.0
94-95	33.004842693241045	34.0	34.0	34.0	34.0	34.0
96-97	32.91748881180065	34.0	34.0	34.0	34.0	34.0
98-99	32.98748290558572	34.0	34.0	34.0	34.0	34.0
100-101	32.62259101603894	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	5.0
25	1.0
26	11.0
27	24.0
28	38.0
29	49.0
30	62.0
31	91.0
32	202.0
33	2626.0
34	890.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	26.55	21.875	32.2	19.375
2	32.175	26.375	24.15	17.299999999999997
3	29.725	28.199999999999996	25.05	17.025000000000002
4	27.925	27.500000000000004	26.025	18.55
5	23.05	30.55	25.900000000000002	20.5
6	24.725	29.7	25.55	20.025000000000002
7	25.0	27.625	27.400000000000002	19.975
8	24.8	26.0	27.950000000000003	21.25
9	23.525	26.974999999999998	25.874999999999996	23.625
10-11	23.0625	27.1	28.175	21.6625
12-13	22.925	30.862499999999997	28.299999999999997	17.9125
14-15	20.974999999999998	33.3375	29.6375	16.05
16-17	21.125	30.825000000000003	32.6625	15.387500000000001
18-19	19.43050677370798	31.409934771700954	31.322127446061216	17.837431008529855
20-21	19.39719859929965	30.777888944472238	32.12856428214107	17.696348174087042
22-23	21.8562124248497	30.04759519038076	31.826152304609217	16.27004008016032
24-25	21.40795582883674	30.844522524783535	29.80298657297026	17.94453507340946
26-27	20.791330645161292	30.619959677419356	31.98084677419355	16.607862903225808
28-29	20.273175667130392	31.263437460478055	29.986088276210953	18.4772985961806
30-31	20.522246165546964	32.13335023450374	28.97705666117379	18.367346938775512
32-33	20.715103702761166	31.98880264664716	29.533019468125715	17.763074182465964
34-35	21.262458471760798	31.919243547150522	29.54255047278303	17.275747508305646
36-37	21.162731006160165	33.7782340862423	29.799794661190965	15.259240246406572
38-39	22.064699059157107	31.589122309575977	30.055419512823818	16.2907591184431
40-41	22.08517268242015	33.48480914048299	29.39496234744222	15.035055829654635
42-43	20.433476955216083	33.99921660791226	30.369499934717325	15.197806502154329
44-45	19.435695538057743	33.91076115485564	29.58005249343832	17.07349081364829
46-47	19.944378228049263	33.96901072705602	28.91007813534631	17.176532909548403
48-49	17.752508361204015	34.06020066889632	30.461538461538463	17.725752508361204
50-51	18.130968622100955	34.99317871759891	32.04638472032742	14.829467939972716
52-53	16.57589722105851	35.93073593073593	31.308476469766795	16.184890378438766
54-55	17.68620787312376	32.63947890116115	33.50325686774285	16.171056357972247
56-57	21.271113655883195	30.017177211565986	33.20927569424563	15.502433438305182
58-59	20.20011600928074	29.872389791183295	34.222737819025525	15.70475638051044
60-61	18.243442381373416	29.560860595343353	37.45947539050987	14.736221632773358
62-63	16.090043813264842	30.986553860099715	36.81825049101073	16.105151835624717
64-65	18.088258225479496	30.297832527678153	35.14735693123343	16.466552315608922
66-67	16.77388227725957	32.180765519459634	34.28755226761017	16.757799935670633
68-69	17.105701900633544	31.14371457152384	34.7782594198066	16.972324108036013
70-71	19.483893314859717	33.73744371319709	31.60720471077243	15.171458261170764
72-73	17.547304534094966	34.63048911103178	32.77400928239914	15.048197072474117
74-75	16.786042240587694	34.69237832874196	32.525252525252526	15.996326905417815
76-77	18.11498586239397	33.66635249764373	33.10084825636192	15.117813383600376
78-79	16.950135291843836	32.663316582914575	33.1078469269424	17.27870119829919
80-81	18.696088264794383	31.07321965897693	33.56068204613841	16.67001003009027
82-83	17.895833333333332	30.4375	35.45833333333333	16.208333333333332
84-85	19.479974837492136	32.18704130845041	33.55001048437828	14.782973369679178
86-87	18.709256844850064	30.33463711429813	33.39852238157323	17.557583659278574
88-89	20.374126662159117	30.69641649763354	31.643002028397564	17.286454811809783
90-91	18.930803054848415	29.99305716269382	33.16361953251562	17.912520249942144
92-93	16.8398163807683	30.587098332930662	33.75211403720705	18.820971249093983
94-95	15.873417721518987	33.41772151898734	33.41772151898734	17.29113924050633
96-97	17.34073486650806	33.835580227332805	31.43008194554586	17.39360296061327
98-99	17.8468624064479	29.792746113989637	32.35463442717329	20.00575705238918
100-101	8.25258937520882	36.919478783828936	33.67858336117608	21.149348479786166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.5
17	5.0
18	10.0
19	10.5
20	12.5
21	19.0
22	36.5
23	71.0
24	108.0
25	151.0
26	201.5
27	212.0
28	204.5
29	182.5
30	157.5
31	180.0
32	204.5
33	193.5
34	194.5
35	201.0
36	161.0
37	148.0
38	168.0
39	176.0
40	170.0
41	179.5
42	227.0
43	234.0
44	174.0
45	146.0
46	162.0
47	163.5
48	154.5
49	112.5
50	64.5
51	56.0
52	57.0
53	57.5
54	58.0
55	52.5
56	38.5
57	32.5
58	31.0
59	20.0
60	10.0
61	8.5
62	11.0
63	9.0
64	3.5
65	2.5
66	5.0
67	4.0
68	0.5
69	1.5
70	3.0
71	1.5
72	2.0
73	5.0
74	3.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.35000000000000003
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.46904315196998125
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	6.0
22-23	8.0
24-25	13.0
26-27	16.0
28-29	9.0
30-31	17.0
32-33	13.0
34-35	16.0
36-37	17.0
38-39	28.0
40-41	21.0
42-43	22.0
44-45	33.0
46-47	27.0
48-49	73.0
50-51	83.0
52-53	56.0
54-55	38.0
56-57	42.0
58-59	44.0
60-61	87.0
62-63	90.0
64-65	106.0
66-67	107.0
68-69	117.0
70-71	91.0
72-73	81.0
74-75	60.0
76-77	72.0
78-79	76.0
80-81	131.0
82-83	2.0
84-85	81.0
86-87	83.0
88-89	55.0
90-91	95.0
92-93	82.0
94-95	92.0
96-97	106.0
98-99	196.0
100-101	1608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	50.0823723228995	22.8
2	21.85612300933553	19.900000000000002
3	11.422295442064799	15.6
4	7.5233388248215265	13.700000000000001
5	3.514552443712246	8.0
6	2.2515101592531575	6.15
7	1.043382756727073	3.325
8	0.8237232289950577	3.0
9	0.6040637012630422	2.475
>10	0.8786381109280615	5.050000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	19	0.475	No Hit
CGTTTGACTCTTGTGAAATGGGCCTGTATGATTTTTCTTAATGTAATTAG	16	0.4	No Hit
TGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAA	14	0.35000000000000003	No Hit
AAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	14	0.35000000000000003	No Hit
CATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	13	0.325	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	13	0.325	No Hit
TGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTT	13	0.325	No Hit
TGATAATGGCACACCGGACTCTGTGGGGCTCCCTCCATGTGCTGTCAGGA	12	0.3	No Hit
GGTAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTCCA	12	0.3	No Hit
CAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	12	0.3	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	11	0.27499999999999997	No Hit
GTTAAACAGAGAATTAATACTTACGTATTTGTGGGTAATCAGCTTATCCC	11	0.27499999999999997	No Hit
TCCTCTCAGCATCTTAGGACTGTTTCAAGTAATCATATTGATGAACTCGT	11	0.27499999999999997	No Hit
ATAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGC	11	0.27499999999999997	No Hit
CAGGTGGATTTCTGAGTTCGAGGCCAGCCTGTTCTACAGAGTGAGTTCCT	10	0.25	No Hit
TTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTA	10	0.25	No Hit
TGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCT	9	0.22499999999999998	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
TAGGGCCTGCACAGTTATTCCTGGTTAAGAAGCTGTGGGATGTGACCTAC	9	0.22499999999999998	No Hit
CGCTCCAAACATGTTCTTAAATTTTGCTTATTCCTATTGTGGACTTTGAA	9	0.22499999999999998	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
TCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	9	0.22499999999999998	No Hit
GGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTGC	9	0.22499999999999998	No Hit
TCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTG	9	0.22499999999999998	No Hit
CTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCA	9	0.22499999999999998	No Hit
GAAATTGACCCAAATAATTGATAATGTTTTAATAATGTGAACAACAGTGT	9	0.22499999999999998	No Hit
CCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAAC	9	0.22499999999999998	No Hit
CAAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGAGT	8	0.2	No Hit
CCCAAATAATTGATAATGTTTTAATAATGTGAACAACAGTGTTATGGAAA	8	0.2	No Hit
CAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	8	0.2	No Hit
CAAGACTTGGCTCGCATTTACATTCGACGTACACTTAGAAACTTCATAAA	8	0.2	No Hit
CAGACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAATTAATACTTACG	8	0.2	No Hit
GCTTAGTTCCTAGCATCTACGTGTTATCTCACCACTGTCTATAACTCTAG	8	0.2	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	8	0.2	No Hit
TAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	8	0.2	No Hit
TAGGAGTTGACCTGAATGATGGCAAGCCTCGAAGGGTCTGTTCAAATTCA	8	0.2	No Hit
GGTTGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGT	8	0.2	No Hit
AATCTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAA	8	0.2	No Hit
GGTCTCTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAA	8	0.2	No Hit
GCAGCTCACAACCATCTGAATCTTCAATTTCAGGGAATCCAGTGCCCTCT	8	0.2	No Hit
AGTGCCCTCTCGGATTTACATAAGGAAGTACAATCATGATGATTCAAATA	8	0.2	No Hit
AAAGAAAGAATGAGGAAATGCAAAATGTCAGAACCTCTTTGATTGCTGAG	8	0.2	No Hit
GGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
GAGAAGAATCTAATATACTTAAGGCTGAATTAGACAATGTTACATCTTTGCATCGCCAAGCATAT	7	0.17500000000000002	No Hit
ATCTCCAGTTCTGAGGGTTCTGTCTCTCTCTTATGGCTTCCACAGGCTTC	7	0.17500000000000002	No Hit
TAGATACGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	7	0.17500000000000002	No Hit
GGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTGCG	7	0.17500000000000002	No Hit
AAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACC	7	0.17500000000000002	No Hit
GGGAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
GGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGTGCAT	7	0.17500000000000002	No Hit
AAGGATTCGTTTGACTCTTGTGAAATGGGCCTGTATGATTTTTCTTAATG	7	0.17500000000000002	No Hit
CATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	7	0.17500000000000002	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTAT	7	0.17500000000000002	No Hit
CTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	7	0.17500000000000002	No Hit
TAGAAAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTT	7	0.17500000000000002	No Hit
TATGCTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAGATT	7	0.17500000000000002	No Hit
GGGGTTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTGCG	7	0.17500000000000002	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	7	0.17500000000000002	No Hit
ACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	7	0.17500000000000002	No Hit
AAAGCCTGATCTGTGGGCAATCTAGTGGAGACCTTTTCTCAGTTGAGGTT	7	0.17500000000000002	No Hit
AGTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	6	0.15	No Hit
GGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTC	6	0.15	No Hit
TCCTCTGCAGCACCAGGCACATGTAGTACACAATGAACATAAAAATAATA	6	0.15	No Hit
GTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	6	0.15	No Hit
GGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGGTTTG	6	0.15	No Hit
ATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAA	6	0.15	No Hit
CACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACAGGAGTT	6	0.15	No Hit
TGTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAG	6	0.15	No Hit
TGCTTAGAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAG	6	0.15	No Hit
GCATATTGGTCATGCCTATAGAGGATCAGTTGACACAGATTATGCGGACTGGACAAAACACTT	6	0.15	No Hit
CTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	6	0.15	No Hit
TAAACCGGAAAATTCATGATAATAATGAGAGTTACCAGGTGGGTTTGTCA	6	0.15	No Hit
CCGACATGTGCTGTCAGGAATCTCCAAGACTTGGCTCGCATTTACATTCG	6	0.15	No Hit
ATGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGAT	6	0.15	No Hit
AATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCA	6	0.15	No Hit
TGCTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTT	6	0.15	No Hit
ATGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGAAA	6	0.15	No Hit
AGATGCTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	6	0.15	No Hit
GGGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGTGCATTTCAGAGTT	6	0.15	No Hit
TAGACACAGAAGAAATCTCTACATCTCATACCATTTTTGATTTGTCCTCCAGTGCTGATAAACTACTCT	6	0.15	No Hit
TGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
GGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACC	6	0.15	No Hit
GAGGAAAGAAAGAGAGTTAAACAGAGAATTAATACTTACGTATTTGTGGG	6	0.15	No Hit
TGAGAACTGTGTAAATCATTAAGCTATGCTCTTTGTGTATGGAGGTTGCAAAAAAAGGCACCTCCTTTAGACT	6	0.15	No Hit
GCTTTCTGTGTAGGTGATCAGTTAAAACAGTTTAAAGATGAAATTGACCC	6	0.15	No Hit
CCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGA	6	0.15	No Hit
GAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	6	0.15	No Hit
AAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAAC	6	0.15	No Hit
GGAATCCACAAAGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAG	6	0.15	No Hit
TGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	6	0.15	No Hit
ACCACAGAAGAAATCTCTACATCTCATACCATTTTTGATTTGTCCTCCAGTGCTGATAAACTACTCTAAAAAC	6	0.15	No Hit
TGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTT	6	0.15	No Hit
CGGAAAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTT	6	0.15	No Hit
TAGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	6	0.15	No Hit
TGCTATAGAATTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTA	6	0.15	No Hit
AGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTTCAAACAATCAT	6	0.15	No Hit
GAGGGCGCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAATTAATA	6	0.15	No Hit
TGTTGGTGATCAGTTAAAACAGTTTAAAGATGAAATTGACCCAAATAATT	6	0.15	No Hit
GTGGTAATCAGCTTATCCCTCAGCCTCTAGACAGTGAAGAGGATGAAAAAAT	6	0.15	No Hit
CAAAACTGGAACTACAGTCCCACACGATCTTCCGTTCTTTGCTGGCCTCC	6	0.15	No Hit
CCCGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	5	0.125	No Hit
TCGTGAGGCCTCTAGAGTTAGTGTCTCTTCTGATCCTTAGGTAACTGCCA	5	0.125	No Hit
TCTCGAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGA	5	0.125	No Hit
AGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	5	0.125	No Hit
CTGTCATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAA	5	0.125	No Hit
GGCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGG	5	0.125	No Hit
CATCTGACCAGCATCTGGCCTCCATGTGTGCCTGCATCTGGTCTCCATGT	5	0.125	No Hit
GTGCGTGACTGGACAAAACACTTGGGAAAGTAAAAATATCCTTGCGGTTT	5	0.125	No Hit
TCTATTACTGTTACTTATATTTTTTATGATTTTTGTATTTATTGTCCTCT	5	0.125	No Hit
TTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTT	5	0.125	No Hit
ACAGCGGTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACA	5	0.125	No Hit
TTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAA	5	0.125	No Hit
AATCGGGACCAGTACTCGTTTCACTTAGGGTGGAGGAGAGAGCTCTGATG	5	0.125	No Hit
TGTATCCTGTTTTAAACTTAGTAGTCAGATGTCAATATTTCTTTATTCAT	5	0.125	No Hit
TTTGACTCTTGTGAAATGGGCCTGTATGATTTTTCTTAATGTAATTAGTA	5	0.125	No Hit
GGTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTG	5	0.125	No Hit
AAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	5	0.125	No Hit
ACGCAAGAAAAGGATCAGAAGTTGTTAGAGATGGAAAAGATAATGCATAC	5	0.125	No Hit
TGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTAT	5	0.125	No Hit
GAAGTATGCAGGCCTTTCCAAGGGTCTTGAACCCGAGGAAAAGTTAAGGT	5	0.125	No Hit
AGGATTCGTTTGACTCTTGTGAAATGGGCCTGTATGATTTTTCTTAATGT	5	0.125	No Hit
CGCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTCA	5	0.125	No Hit
TCTGATGAGAAAATCTTTTGTCTTGGACCAGTAGTTCTGAACTTTGGGTC	5	0.125	No Hit
AAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCT	5	0.125	No Hit
TTTCAGCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAA	5	0.125	No Hit
GCTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGT	5	0.125	No Hit
TGTCATTGGTGGACTGCACTTACCTTTTAAGTGGCTACATCTTAGGAACAATAAATTTATTAAAATTCTTGGCT	5	0.125	No Hit
GTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAA	5	0.125	No Hit
AGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGAAAGAGA	5	0.125	No Hit
GAAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGA	5	0.125	No Hit
ATCTCTGCCTTGAAGTATGTATGAGATTGCAAAAGAGTTGGTAGCAGTGT	5	0.125	No Hit
TGGTCATGCCTATAGAGGATCAGTTGACACAGATTATGCGGACTGGACAA	5	0.125	No Hit
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	5	0.125	No Hit
TTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	5	0.125	No Hit
AAGATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GGGTGCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATA	5	0.125	No Hit
ATTCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	5	0.125	No Hit
TCGTTTGACTCTTGTGAAATGGGCCTGTATGATTTTTCTTAATGTAATTA	5	0.125	No Hit
TAGTAACCATCTGTAATGGGATCTGATGCCCTCTTTTGGTGTCTGAAGAC	5	0.125	No Hit
CACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAAT	5	0.125	No Hit
AAGATGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAA	5	0.125	No Hit
GAAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCT	5	0.125	No Hit
GCCTTATACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	5	0.125	No Hit
GGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTC	5	0.125	No Hit
GCAGATGCTAGAATCTCATGCCTCAACTATTCAGGAAAAAGAGCAACAGC	5	0.125	No Hit
GTCCGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
ATACTTCGACGTACACTTAGAAACTTCATAAATGATGAGATGCAGGCCAA	5	0.125	No Hit
GGCACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTT	5	0.125	No Hit
CCTGAGCTACATAGTGAGTTCTAGGCCAGCCTTGGCTACACTTAAAATCCTGTCCT	5	0.125	No Hit
ACAGCACCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGC	5	0.125	No Hit
CCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	5	0.125	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTGTTCCTCAGAAATGCCAGATAAAATAATACAAGATTGAGAGAAAACTC	5	0.125	No Hit
AGGATGATGTAGCACCTGTGTGACCCCAGCATACAGGAATTCAAATATCC	5	0.125	No Hit
CTTTTCTGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAG	5	0.125	No Hit
TCGTTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
TTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
GAGAGTTAAACAGAGAATTAATACTTACGTATTTGTGGGTAATCAGCTTA	5	0.125	No Hit
GATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGG	5	0.125	No Hit
CCTTGACCTGGTTTTCACACATATGTACACATCTATACAAACACATGCATAGACCACACAACCACACC	5	0.125	No Hit
AGGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTG	5	0.125	No Hit
TACGCGATGAACATAAAAATAATAAAACCAAATCTAATTTTAAATAATTA	5	0.125	No Hit
CGAGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAA	5	0.125	No Hit
TATTTCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323885 spots for SRR25194570.sra
Written 1323885 spots for SRR25194570.sra
Read 1323889 spots for SRR25194570.sra
Written 1323889 spots for SRR25194570.sra
SRR ids: ['SRR25194570.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_v2bz74df
SRR25194570.sra spots: 10591084
blocks: [[1, 1323885], [1323886, 2647770], [2647771, 3971655], [3971656, 5295540], [5295541, 6619425], [6619426, 7943310], [7943311, 9267195], [9267196, 10591084]]
SRR25194570 file size 2260976
SRR25194570 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25194570 SRR25194570_1.fastq
Input file:	SRR25194570_1.fastq
trimmed:	SRR25194570-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Jun 21 07:17:58 2026 >> started

Sun Jun 21 07:18:01 2026 >> done (2.960s)
10591084 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
10591084 (100.00%) reads available; of these:
      60 ( 0.00%) trimmed reads available after processing
10591024 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    8714	  0.08%
 21	    8072	  0.08%
 22	    8247	  0.08%
 23	    8251	  0.08%
 24	    8455	  0.08%
 25	    9236	  0.09%
 26	    9764	  0.09%
 27	   11239	  0.11%
 28	   12384	  0.12%
 29	   14099	  0.13%
 30	   15942	  0.15%
 31	   18712	  0.18%
 32	   21386	  0.20%
 33	   22039	  0.21%
 34	   21725	  0.21%
 35	   22601	  0.21%
 36	   23684	  0.22%
 37	   25204	  0.24%
 38	   27442	  0.26%
 39	   29746	  0.28%
 40	   31464	  0.30%
 41	   32647	  0.31%
 42	   36441	  0.34%
 43	   40452	  0.38%
 44	   42407	  0.40%
 45	   40179	  0.38%
 46	   40346	  0.38%
 47	   40399	  0.38%
 48	   41223	  0.39%
 49	   43329	  0.41%
 50	   45450	  0.43%
 51	   48222	  0.46%
 52	   52469	  0.50%
 53	   55624	  0.53%
 54	   57112	  0.54%
 55	   63418	  0.60%
 56	   77099	  0.73%
 57	   77536	  0.73%
 58	   76063	  0.72%
 59	   69088	  0.65%
 60	   67435	  0.64%
 61	   67571	  0.64%
 62	   67749	  0.64%
 63	   67969	  0.64%
 64	   70016	  0.66%
 65	   70512	  0.67%
 66	   74094	  0.70%
 67	   76853	  0.73%
 68	   78980	  0.75%
 69	   82479	  0.78%
 70	   85085	  0.80%
 71	   88698	  0.84%
 72	   89382	  0.84%
 73	   84471	  0.80%
 74	   83280	  0.79%
 75	   82884	  0.78%
 76	   82515	  0.78%
 77	   82739	  0.78%
 78	   86716	  0.82%
 79	   95913	  0.91%
 80	  119469	  1.13%
 81	  144354	  1.36%
 82	     686	  0.01%
 83	   17040	  0.16%
 84	   65229	  0.62%
 85	   77951	  0.74%
 86	   80056	  0.76%
 87	   82334	  0.78%
 88	   84052	  0.79%
 89	   84022	  0.79%
 90	   81774	  0.77%
 91	   90526	  0.85%
 92	   55558	  0.52%
 93	   59392	  0.56%
 94	   77941	  0.74%
 95	  100200	  0.95%
 96	  119215	  1.13%
 97	  190364	  1.80%
 98	  305652	  2.89%
 99	  189827	  1.79%
100	  820831	  7.75%
101	 4821360	 45.52%
10591084 reads passed initial QC


criterion=sequence-density
sequence-density=0.53
sequence-density-rank=1
fanout-score=6.31
fanout-score-rank=26
prefix-density=1.62
prefix-fanout=2.1
sequence=TTAATCTTTGCACCTTTTCCTACTTTGGTGTTTGTGAATAGAAGTAAAAATAAATACGACTGCCACCTCACG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=86.45
fanout-score-rank=1
prefix-density=0.57
prefix-fanout=1.9
sequence=CCTCTTCCTGCTCTGGGACCAGCAACACAGGTTCCCAGTCTTCATGGGTCGTGTATATGACCCCAGGGGTTGAGACAGGCTTGGGTAAACATTGTCACCCAAGCTTCAGCTCCTCCGGTTATTTCCTTGCCACTGCCTGCCCGAGCCACTTCAAGCCTTAGGAACTGGCAGACGGAACTGTTTCCATCCACCAACCCCCAGGGTATCAACCACTTTTTTGCAGCTTTTACGGTTCAAACCTATCAAACTCTACAAATAAAACTTGCAGACATTTTCTTCTCTCACTAA
                                 Started job on |	Jun 21 07:18:06
                             Started mapping on |	Jun 21 07:18:06
                                    Finished on |	Jun 21 07:18:16
       Mapping speed, Million of reads per hour |	3812.79

                          Number of input reads |	10591084
                      Average input read length |	87
                                    UNIQUE READS:
                   Uniquely mapped reads number |	9062455
                        Uniquely mapped reads % |	85.57%
                          Average mapped length |	86.49
                       Number of splices: Total |	928509
            Number of splices: Annotated (sjdb) |	919750
                       Number of splices: GT/AG |	922820
                       Number of splices: GC/AG |	3100
                       Number of splices: AT/AC |	261
               Number of splices: Non-canonical |	2328
                      Mismatch rate per base, % |	0.44%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.42
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1437375
             % of reads mapped to multiple loci |	13.57%
        Number of reads mapped to too many loci |	46185
             % of reads mapped to too many loci |	0.44%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.42%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	91254	91254	91254
N_multimapping	1437375	1437375	1437375
N_noFeature	656740	686601	8284571
N_ambiguous	843629	96072	526092
UnstrandedReadsAssigned:7562086 PositiveStrandReadsAssigned:8279782 NegativeStrandReadsAssigned:251792
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=75 echo kmer=71
SRR25194570 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194570-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 10,591,084 reads, 6,584,768 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,312 rounds

 109283 SRR25194570.ke.tsv
  52636 SRR25194570.se.tsv
 161919 total
==> SRR25194570.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194570.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194570 completed mapping pipeline successfully
