Starting /dee2/code/volunteer_pipeline.sh SRR25194575
    current disk space = 1248535972
    free memory = 200716040 
SRR25194575 SRAfilesize
64cad0ccc86a9fcd6994636898167ac9  SRR25194575.sra
SRR25194575.sra file validated
SRR25194575 is single end
SRR25194575 is conventional basespace
SRR25194575 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.3155	34.0	34.0	34.0	34.0	34.0
2	33.454	34.0	34.0	34.0	34.0	34.0
3	33.44	34.0	34.0	34.0	34.0	34.0
4	33.477	34.0	34.0	34.0	34.0	34.0
5	33.6095	34.0	34.0	34.0	34.0	34.0
6	33.534	34.0	34.0	34.0	34.0	34.0
7	33.6615	34.0	34.0	34.0	34.0	34.0
8	33.6075	34.0	34.0	34.0	34.0	34.0
9	33.5325	34.0	34.0	34.0	34.0	34.0
10-11	33.62875	34.0	34.0	34.0	34.0	34.0
12-13	33.665	34.0	34.0	34.0	34.0	34.0
14-15	33.65375	34.0	34.0	34.0	34.0	34.0
16-17	33.60225	34.0	34.0	34.0	34.0	34.0
18-19	33.515249999999995	34.0	34.0	34.0	34.0	34.0
20-21	33.64356702580807	34.0	34.0	34.0	34.0	34.0
22-23	33.59784244856999	34.0	34.0	34.0	34.0	34.0
24-25	33.55789122883717	34.0	34.0	34.0	34.0	34.0
26-27	33.68325903881426	34.0	34.0	34.0	34.0	34.0
28-29	33.626084109413384	34.0	34.0	34.0	34.0	34.0
30-31	33.68707040465935	34.0	34.0	34.0	34.0	34.0
32-33	33.62747675812861	34.0	34.0	34.0	34.0	34.0
34-35	33.67365974402318	34.0	34.0	34.0	34.0	34.0
36-37	33.6776139697989	34.0	34.0	34.0	34.0	34.0
38-39	33.65001514794493	34.0	34.0	34.0	34.0	34.0
40-41	33.61975968859225	34.0	34.0	34.0	34.0	34.0
42-43	33.59661970476253	34.0	34.0	34.0	34.0	34.0
44-45	33.56554737886419	34.0	34.0	34.0	34.0	34.0
46-47	33.54510920818393	34.0	34.0	34.0	34.0	34.0
48-49	33.539779631345894	34.0	34.0	34.0	34.0	34.0
50-51	33.515936241141006	34.0	34.0	34.0	34.0	34.0
52-53	33.54477009562824	34.0	34.0	34.0	34.0	34.0
54-55	33.541497088312106	34.0	34.0	34.0	34.0	34.0
56-57	33.54290023644042	34.0	34.0	34.0	34.0	34.0
58-59	33.428406236848176	34.0	34.0	34.0	34.0	34.0
60-61	33.44270456773983	34.0	34.0	34.0	34.0	34.0
62-63	33.44288968079794	34.0	34.0	34.0	34.0	34.0
64-65	33.40089083072052	34.0	34.0	34.0	34.0	34.0
66-67	33.403245451621984	34.0	34.0	34.0	34.0	34.0
68-69	33.291256201444085	34.0	34.0	34.0	34.0	34.0
70-71	33.25846852451504	34.0	34.0	34.0	34.0	34.0
72-73	33.23669922109892	34.0	34.0	34.0	34.0	34.0
74-75	33.19306904767685	34.0	34.0	34.0	34.0	34.0
76-77	33.141541162574455	34.0	34.0	34.0	34.0	34.0
78-79	33.112570031811146	34.0	34.0	34.0	34.0	34.0
80-81	33.04238480081858	34.0	34.0	34.0	34.0	34.0
82-83	33.11683357176919	34.0	34.0	34.0	34.0	34.0
84-85	33.066642650217474	34.0	34.0	34.0	34.0	34.0
86-87	33.006517952325495	34.0	34.0	34.0	34.0	34.0
88-89	33.04893796534573	34.0	34.0	34.0	34.0	34.0
90-91	32.958593534568095	34.0	34.0	34.0	34.0	34.0
92-93	32.89653674265325	34.0	34.0	34.0	34.0	34.0
94-95	32.938654526333046	34.0	34.0	34.0	34.0	34.0
96-97	32.89727144721478	34.0	34.0	34.0	34.0	34.0
98-99	32.929838807689684	34.0	34.0	34.0	34.0	34.0
100-101	32.71350607876306	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	2.0
25	5.0
26	8.0
27	24.0
28	37.0
29	36.0
30	41.0
31	77.0
32	185.0
33	2469.0
34	1115.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.599999999999998	22.925	33.45	20.025000000000002
2	30.475	24.95	26.35	18.224999999999998
3	29.725	27.675	23.775	18.825
4	26.05	29.15	28.199999999999996	16.6
5	26.35	32.300000000000004	24.8	16.55
6	24.45	28.275	29.475	17.8
7	24.5	27.450000000000003	31.324999999999996	16.725
8	26.375	24.775	29.5	19.35
9	23.674999999999997	25.025	30.725	20.575
10-11	23.425	27.5625	28.599999999999998	20.4125
12-13	21.55	29.525000000000002	31.45	17.474999999999998
14-15	20.3875	32.1625	33.425	14.025000000000002
16-17	21.099999999999998	29.45	35.862500000000004	13.5875
18-19	20.962285427891242	27.20210499937351	36.68713193835359	15.148477634381658
20-21	20.47303216118133	27.75622575397322	35.790264047052936	15.980478037792517
22-23	21.45007526342198	29.302558956347212	34.119417962870045	15.127947817360763
24-25	20.97524192534875	27.761719240919945	35.67927610908634	15.583762724644966
26-27	20.03021528389777	28.402366863905325	36.17021276595745	15.397205086239458
28-29	18.721633888048412	29.475542107917295	34.39233484619263	17.410489157841656
30-31	19.74763406940063	30.889589905362776	31.760252365930597	17.602523659305994
32-33	21.416824794433904	28.71600253004428	33.97849462365591	15.888678051865906
34-35	17.803174603174604	33.371428571428574	33.0031746031746	15.822222222222221
36-37	21.068741231985715	31.679632699910727	30.302257365131997	16.949368702971558
38-39	21.58974358974359	32.67948717948718	30.333333333333336	15.3974358974359
40-41	22.560740455071347	32.214937652654584	30.376655097056176	14.847666795217895
42-43	19.10068679538681	35.62265128936115	30.94466761694959	14.33199429830245
44-45	18.004196170993968	35.20849724626279	30.89430894308943	15.892997639653817
46-47	17.057033214238455	35.56967050416832	31.044065105200474	16.329231176392746
48-49	16.764548852108916	36.53230112119594	30.979711692471973	15.723438334223172
50-51	15.889628924833493	34.2938697838793	35.31330705450591	14.503194236781297
52-53	14.44490876166597	36.2724613455913	35.39490179690765	13.887728095835076
54-55	17.235519048293444	32.445829202662516	37.06273898881178	13.255912760232262
56-57	19.744032211676732	28.15645671555939	39.32988208225482	12.76962899050906
58-59	20.477716283134285	26.52199242644917	40.25633556655986	12.743955723856685
60-61	18.46084743225034	25.84219194490193	41.45830214103908	14.238658481808656
62-63	17.887078918235012	25.430966313458804	41.54673414518425	15.135220623121937
64-65	17.01279282272803	30.220966938029576	36.25186908124273	16.51437115799967
66-67	17.766051011433596	27.810026385224273	38.34652594547054	16.077396657871592
68-69	18.625644804716284	29.587324981577005	37.159174649963155	14.627855563743552
70-71	19.449326303456356	31.673501269283342	34.153485647334506	14.723686779925796
72-73	17.628010524185385	30.35822707953855	36.45011131349929	15.563651082776767
74-75	18.842390172808663	33.06266916510514	34.54091193004372	13.554028732042472
76-77	17.580750054194667	32.56015608064167	33.55733795794494	16.30175590721873
78-79	16.376617581436857	29.808121374386438	37.39402052655065	16.42124051762606
80-81	15.945065176908752	30.609869646182496	35.498137802607076	17.946927374301676
82-83	17.525035765379112	32.30805913209347	34.59704339532666	15.569861707200763
84-85	16.41862698031685	34.493518963034084	34.661545847335574	14.42630820931349
86-87	18.8833746898263	31.19106699751861	32.8287841191067	17.096774193548388
88-89	18.319630010277493	29.624871531346354	33.47893114080164	18.57656731757451
90-91	16.82365253793825	30.429094714809	34.58922030350602	18.15803244374673
92-93	18.83505715841045	29.586281981491563	37.778987479586284	13.799673380511704
94-95	18.124098124098122	30.822510822510825	32.55411255411255	18.4992784992785
96-97	14.92224880382775	30.92105263157895	35.58612440191388	18.570574162679428
98-99	16.91911053818885	29.4231388978408	33.67708669029971	19.980663873670643
100-101	9.670409272002898	36.29119884099964	34.55269829771822	19.485693589279247
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.5
16	2.0
17	2.5
18	4.0
19	7.0
20	11.0
21	39.0
22	56.0
23	61.0
24	129.5
25	248.0
26	353.5
27	393.0
28	337.0
29	283.0
30	265.5
31	190.5
32	128.0
33	118.5
34	95.0
35	95.5
36	110.0
37	115.5
38	133.5
39	145.0
40	154.0
41	181.5
42	190.5
43	185.5
44	202.5
45	161.0
46	148.5
47	198.5
48	188.0
49	120.0
50	81.5
51	57.0
52	24.5
53	28.0
54	41.5
55	40.5
56	27.0
57	19.0
58	13.5
59	8.5
60	3.0
61	0.5
62	0.0
63	5.5
64	8.0
65	5.0
66	5.0
67	5.0
68	2.5
69	0.0
70	2.5
71	2.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.2375
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.2594594594594595
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	14.0
22-23	6.0
24-25	7.0
26-27	6.0
28-29	3.0
30-31	7.0
32-33	18.0
34-35	14.0
36-37	21.0
38-39	12.0
40-41	19.0
42-43	51.0
44-45	33.0
46-47	32.0
48-49	51.0
50-51	101.0
52-53	61.0
54-55	52.0
56-57	52.0
58-59	63.0
60-61	167.0
62-63	159.0
64-65	177.0
66-67	136.0
68-69	144.0
70-71	111.0
72-73	60.0
74-75	90.0
76-77	76.0
78-79	77.0
80-81	83.0
82-83	0.0
84-85	63.0
86-87	79.0
88-89	34.0
90-91	67.0
92-93	96.0
94-95	70.0
96-97	69.0
98-99	148.0
100-101	1471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.5369532428356	12.775
2	20.28657616892911	13.450000000000001
3	11.840120663650074	11.774999999999999
4	7.8431372549019605	10.4
5	7.390648567119156	12.25
6	4.374057315233785	8.7
7	2.790346907993967	6.4750000000000005
8	2.1870286576168927	5.800000000000001
9	1.0558069381598794	3.15
>10	3.695324283559578	15.225
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	32	0.8	No Hit
CACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	19	0.475	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	17	0.42500000000000004	No Hit
TTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	16	0.4	No Hit
CTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	16	0.4	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	16	0.4	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	15	0.375	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	15	0.375	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	15	0.375	No Hit
GCGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGA	14	0.35000000000000003	No Hit
CATTAATTTTCTGTTCTACTCTCTTCACTACTAAAAAAGAACATGTTTCT	14	0.35000000000000003	No Hit
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	13	0.325	No Hit
ATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	13	0.325	No Hit
TACTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTG	13	0.325	No Hit
AACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAA	13	0.325	No Hit
CCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTT	13	0.325	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	13	0.325	No Hit
AGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGA	12	0.3	No Hit
TCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTG	12	0.3	No Hit
CTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGC	12	0.3	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	12	0.3	No Hit
TACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	12	0.3	No Hit
AGATATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	11	0.27499999999999997	No Hit
GCCAATCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTC	11	0.27499999999999997	No Hit
TACAAAACCAAACAGGTAGAAGTCCTTATAGAAAAACAGGAAAACACATC	11	0.27499999999999997	No Hit
CTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	11	0.27499999999999997	No Hit
TGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAG	11	0.27499999999999997	No Hit
GTGTAGGTGTCTCTGGAAGCAGTGAGTAGAGTCACTGTTTTAATTTCTTC	11	0.27499999999999997	No Hit
CAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	11	0.27499999999999997	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	11	0.27499999999999997	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	11	0.27499999999999997	No Hit
CCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	11	0.27499999999999997	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	11	0.27499999999999997	No Hit
TGGATGTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAA	11	0.27499999999999997	No Hit
CGAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATC	10	0.25	No Hit
CGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	10	0.25	No Hit
TCCGTTCTTTGCTGGCCTCCACGGGGACTGTATGAACATGGTACACAGAC	10	0.25	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	10	0.25	No Hit
GGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
TGACCTTCCATGGATTTTCGGCAAGATACTCAATACACATAAAATAATAA	10	0.25	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
CGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCAT	10	0.25	No Hit
AAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	10	0.25	No Hit
AGACGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTAT	10	0.25	No Hit
TGGTATGGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCA	10	0.25	No Hit
TTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTA	10	0.25	No Hit
TTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	10	0.25	No Hit
AATAGAGTCAGATTAACTGTTGGTTCTGAGCAGTTCTTTGAGAGGGAACT	10	0.25	No Hit
TGTCAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
CATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	9	0.22499999999999998	No Hit
CTGGGCATGTGTCCTTGTTATATGTTGGAGAATGACACATCAAAACGACC	9	0.22499999999999998	No Hit
GACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	9	0.22499999999999998	No Hit
CGAGGCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAGG	9	0.22499999999999998	No Hit
CCTAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	9	0.22499999999999998	No Hit
CAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGT	9	0.22499999999999998	No Hit
GTGGGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATC	9	0.22499999999999998	No Hit
GAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	9	0.22499999999999998	No Hit
AGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGG	9	0.22499999999999998	No Hit
CTGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	9	0.22499999999999998	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
GGGAGAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
AAGAAATTTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	9	0.22499999999999998	No Hit
TGTATAATATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	8	0.2	No Hit
AACGAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACA	8	0.2	No Hit
CTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	8	0.2	No Hit
TGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTT	8	0.2	No Hit
CAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAA	8	0.2	No Hit
TTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAA	8	0.2	No Hit
TCCAATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	8	0.2	No Hit
TGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAA	8	0.2	No Hit
GTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGAT	8	0.2	No Hit
TGGTAATTCGTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAAT	8	0.2	No Hit
TGAGAAACCACTGCAGGGTGTTGCTGGATGTTTAAAGCTACCCTGAGCTA	8	0.2	No Hit
GGGAAAATGGCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	8	0.2	No Hit
GACTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	8	0.2	No Hit
AACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACA	8	0.2	No Hit
CTGCTCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	8	0.2	No Hit
TCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCT	8	0.2	No Hit
TAAGGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAAC	8	0.2	No Hit
GTTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGT	8	0.2	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	8	0.2	No Hit
GATCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	8	0.2	No Hit
TAGATTCTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATG	8	0.2	No Hit
AACGAAGGGGTATAGAAATTAAATAGCTTGTGGAATAGTGTTCTGTTGTA	8	0.2	No Hit
TTTGTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAA	8	0.2	No Hit
ATGAGGGAATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGA	8	0.2	No Hit
AAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	8	0.2	No Hit
TAAGTATGCAGGCCTTTCCAAGGGTCTTGAACCCGAGGAAAAGTTAAGGT	8	0.2	No Hit
ATACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCT	8	0.2	No Hit
CTATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAAT	8	0.2	No Hit
TGAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	8	0.2	No Hit
TGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAA	7	0.17500000000000002	No Hit
AAATATGCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	7	0.17500000000000002	No Hit
ACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	7	0.17500000000000002	No Hit
TAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGAC	7	0.17500000000000002	No Hit
TTCTGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATT	7	0.17500000000000002	No Hit
TATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCAC	7	0.17500000000000002	No Hit
GTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAGGAAT	7	0.17500000000000002	No Hit
TAAGGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGT	7	0.17500000000000002	No Hit
CAAGGATGCTATTGTCTGTGTGCAGCAAGAAAAGGATCAGAAGTTGTTAG	7	0.17500000000000002	No Hit
CGGACTGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGGTTT	7	0.17500000000000002	No Hit
AAACACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	7	0.17500000000000002	No Hit
TTACGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	7	0.17500000000000002	No Hit
TGATACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	7	0.17500000000000002	No Hit
TATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACA	7	0.17500000000000002	No Hit
GCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGCCTTCACTGCTC	7	0.17500000000000002	No Hit
CCATGCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCT	7	0.17500000000000002	No Hit
ATCGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGC	7	0.17500000000000002	No Hit
GCAGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATC	7	0.17500000000000002	No Hit
AACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	7	0.17500000000000002	No Hit
AGGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	7	0.17500000000000002	No Hit
TTCTCACAACCACCTGTAATGAGATCTGACACCCACTTCTGGTGCGTCAG	7	0.17500000000000002	No Hit
TTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAA	7	0.17500000000000002	No Hit
ATAATGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	7	0.17500000000000002	No Hit
CTCTGTAAAATGAATATAATTAAAGGATTCTCACTCCAAACATGTTCTTA	7	0.17500000000000002	No Hit
TCGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
GTTTTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTG	7	0.17500000000000002	No Hit
TTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	7	0.17500000000000002	No Hit
TTGGATCGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTC	7	0.17500000000000002	No Hit
AAGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAG	7	0.17500000000000002	No Hit
GTGAGCCAGACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACC	7	0.17500000000000002	No Hit
CACCTGTGATCCCAGTACTTGAGAAACCACTGCAGGGTGTTGCTGGATGT	7	0.17500000000000002	No Hit
GCGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
ACTTCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCA	7	0.17500000000000002	No Hit
CTGAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTG	7	0.17500000000000002	No Hit
GCAGTTAGTTAAATAGTTAGCAGTTAGACTAATTTAGAACATATGACATA	7	0.17500000000000002	No Hit
GGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGT	7	0.17500000000000002	No Hit
TGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	7	0.17500000000000002	No Hit
TTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAAT	6	0.15	No Hit
GGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATT	6	0.15	No Hit
AACAACGGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
AAATGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	6	0.15	No Hit
TCTCATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGT	6	0.15	No Hit
ACTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
CGTGACGTCAACTCGGCCGATGGGCTGCAGCCAATCAGGGAGTGACACGT	6	0.15	No Hit
CATCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACT	6	0.15	No Hit
AGATACAGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	6	0.15	No Hit
TAATCTCAGCACTTGGGAGGCAGAGGCAGGTGAATTTCTGAGTTCGAGGA	6	0.15	No Hit
ATTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	6	0.15	No Hit
GCAAATAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAG	6	0.15	No Hit
TTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAGATTTATTTG	6	0.15	No Hit
ATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAA	6	0.15	No Hit
AAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATT	6	0.15	No Hit
TGCTTAGAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAG	6	0.15	No Hit
ACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTT	6	0.15	No Hit
TGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CCTGAACGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	6	0.15	No Hit
AATTCTGTGGGCAATCTAGTGGAGACCTTTTCTCAGTTGAGGTTTCTCTT	6	0.15	No Hit
CTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCT	6	0.15	No Hit
TGGAAAAGATAATGCATACTCAACATTGTGAAATTAAAGAACTGAAGCAG	6	0.15	No Hit
TAGTTTGAAAGAATTAGTTTAAAAGTAATAAGGTTAAAATAGTCCCACTT	6	0.15	No Hit
AGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTG	6	0.15	No Hit
AGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAATT	6	0.15	No Hit
TCCAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCG	6	0.15	No Hit
GCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCCGGAGCCATGGA	6	0.15	No Hit
AACGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TGTCGCCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAG	6	0.15	No Hit
CTTGGGATTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCT	6	0.15	No Hit
TACGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	6	0.15	No Hit
AATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATT	6	0.15	No Hit
AATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGG	6	0.15	No Hit
CTGGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	6	0.15	No Hit
CCCGACAATTTATGAATATACCTTCTTGTTCAGTAAAATTTAAGTTATAA	6	0.15	No Hit
TGGGAAAGTGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
ACAGACTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	6	0.15	No Hit
TCAATCAGTGGAGACCTTTTCTCAGTTGAGGTTTCTCTTACCAGATAACT	6	0.15	No Hit
CAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	6	0.15	No Hit
CGGATGTTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TCCGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	6	0.15	No Hit
GGAGAGCATAGCAAACTGGAGTCCAGAGAAAGTGGGTAGAGGAAGCAGCT	6	0.15	No Hit
TTGCGCTATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	6	0.15	No Hit
GGTTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGG	6	0.15	No Hit
ATGGGGGGCCTTTGGGATAGCATTGAAAATGTAAATGAGGAAAATACCTAATTAAAAAAAAACT	6	0.15	No Hit
TCGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
AGGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
CACTTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATG	6	0.15	No Hit
GTCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCAC	6	0.15	No Hit
TTAAGCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTA	6	0.15	No Hit
TTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	6	0.15	No Hit
AATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATTAGA	6	0.15	No Hit
TTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TGCTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TCACTTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGT	6	0.15	No Hit
TTGCGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	5	0.125	No Hit
AAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
GGGCGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGG	5	0.125	No Hit
GGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAATT	5	0.125	No Hit
GCTCGGTTAGCTTAAATGGCATAGCCTTTTTCTTTCCTTCCTCTTCCAAG	5	0.125	No Hit
TGGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCA	5	0.125	No Hit
AATGTGGAAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GGTCGCTGCTTGGGTTATCCACGGGACTCCATTAAAGTACAACAGAAGAG	5	0.125	No Hit
GGGATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCA	5	0.125	No Hit
TGGAGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
CCCAACTCCTCCCAGATGTCCTCTTCTCTAACCCATCCAACTTCATGGTCTTAAGT	5	0.125	No Hit
GGGATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCAC	5	0.125	No Hit
TTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATG	5	0.125	No Hit
GCCAGGTAAAATGGTGTAAATACATTTTCTAGACCTACACCTGAATTATA	5	0.125	No Hit
GAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	5	0.125	No Hit
TATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
TCTGGAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTA	5	0.125	No Hit
AATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	5	0.125	No Hit
TGGAGCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCG	5	0.125	No Hit
AGCTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTC	5	0.125	No Hit
TACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCAT	5	0.125	No Hit
ATGCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
GATCTGCCTGCCTCTACTGAGTGCTGGGATTAAAGGCTTATGGCACTACC	5	0.125	No Hit
TACGAAATGTTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	5	0.125	No Hit
TTGTGATATATTACTACACTTGGGAGGCAGAGGCAGGTGGATTTCTGAGT	5	0.125	No Hit
AACAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACAC	5	0.125	No Hit
AGTAAGGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GGATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGA	5	0.125	No Hit
ACGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
CAAAAGCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	5	0.125	No Hit
GGCTGGCATACCTATTAGATCATCTAAAATAAAGTGATGATAATGGTGCT	5	0.125	No Hit
TAGAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCAC	5	0.125	No Hit
AACTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAG	5	0.125	No Hit
ACTGCCTGAATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTT	5	0.125	No Hit
CTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTAACTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GGCGAGGGCCTGATGCTACA	5	0.125	No Hit
ACCTCGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	5	0.125	No Hit
TGCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	5	0.125	No Hit
CAAGCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACAGGAGT	5	0.125	No Hit
TAAAGATGGCTTTAAGTTATTTGAGTTACTCAGTAAGTTAAGGTGGTCTTGGGAATCATAGCGAGAACATGTCTT	5	0.125	No Hit
TGGGAATGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	5	0.125	No Hit
GCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACC	5	0.125	No Hit
ATGTGTGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
TATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATG	5	0.125	No Hit
CATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAA	5	0.125	No Hit
TCGGAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	5	0.125	No Hit
CAGCTGCTTCTCCAAGAATAGAAAGTACAACAGGCATTACAACCACTACCTCACC	5	0.125	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	5	0.125	No Hit
TGCTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	5	0.125	No Hit
TGTCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
GCTCTTCGCCTGCTTGCCGTGTGAGACTGAGTAACTGCTAGATCCTTGGA	5	0.125	No Hit
AGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGA	5	0.125	No Hit
TGGAGGCTAGAGTTGTAGGCTACAGAAGCCAGAGAAACATGTAGATGCCT	5	0.125	No Hit
TATCTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	5	0.125	No Hit
GCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGA	5	0.125	No Hit
CGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTC	5	0.125	No Hit
TGAAGGGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGGTTTGAAACTT	5	0.125	No Hit
CTGTTTTTCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATTGAT	5	0.125	No Hit
TTACGGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
GCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATA	5	0.125	No Hit
TGGTCGTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAA	5	0.125	No Hit
GTCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
TGAAGTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	5	0.125	No Hit
AGGCGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGG	5	0.125	No Hit
TGTACAGCTGTAGAAATAAGAAATATTATTGAAAAAGTAAAATGTTCTCT	5	0.125	No Hit
ATTGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	5	0.125	No Hit
GGCTACACGAGATTTTTGTTTTAAATGCCTTGAGGGACTTGCTCCCCACA	5	0.125	No Hit
TAAAGATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	5	0.125	No Hit
GATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	5	0.125	No Hit
CTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGAATT	5	0.125	No Hit
AGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAA	5	0.125	No Hit
CCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAAATAAA	5	0.125	No Hit
GAAGCCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTT	5	0.125	No Hit
AGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGA	5	0.125	No Hit
GTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCA	5	0.125	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
ATGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TACTATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
GGCAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCAC	5	0.125	No Hit
ATCCACCAGGCCCAGACCTCTTTTTAAAAGTTTTATTGAGGGGGAAGAAT	5	0.125	No Hit
AAAACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
GGATTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGCCTTC	5	0.125	No Hit
CAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTT	5	0.125	No Hit
ACAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAATG	5	0.125	No Hit
ACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	5	0.125	No Hit
TTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCT	5	0.125	No Hit
CCTTCAGTCAGCTGCTTCTCCAAGAATAGAAAGTACAACAGGCATTACAACCACTACCTCACC	5	0.125	No Hit
GCGCGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCG	5	0.125	No Hit
TGATTACAGGAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGC	5	0.125	No Hit
GAGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
ACGGCTACTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGC	5	0.125	No Hit
TATATGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACA	5	0.125	No Hit
ATCCATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCTTG	10	0.0040378226	170.02856	92-93
>>END_MODULE
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723957 spots for SRR25194575.sra
Written 1723957 spots for SRR25194575.sra
Read 1723961 spots for SRR25194575.sra
Written 1723961 spots for SRR25194575.sra
SRR ids: ['SRR25194575.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tx_z8q09
SRR25194575.sra spots: 13791660
blocks: [[1, 1723957], [1723958, 3447914], [3447915, 5171871], [5171872, 6895828], [6895829, 8619785], [8619786, 10343742], [10343743, 12067699], [12067700, 13791660]]
SRR25194575 file size 2966072
SRR25194575 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25194575 SRR25194575_1.fastq
Input file:	SRR25194575_1.fastq
trimmed:	SRR25194575-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Jun 21 06:30:24 2026 >> started

Sun Jun 21 06:30:27 2026 >> done (3.804s)
13791660 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
13791660 (100.00%) reads available; of these:
      99 ( 0.00%) trimmed reads available after processing
13791561 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    7131	  0.05%
 21	    7003	  0.05%
 22	    7158	  0.05%
 23	    7541	  0.05%
 24	    8352	  0.06%
 25	    8983	  0.07%
 26	    9847	  0.07%
 27	   11638	  0.08%
 28	   13121	  0.10%
 29	   14514	  0.11%
 30	   17364	  0.13%
 31	   20097	  0.15%
 32	   22819	  0.17%
 33	   23640	  0.17%
 34	   23568	  0.17%
 35	   24504	  0.18%
 36	   26430	  0.19%
 37	   28284	  0.21%
 38	   31040	  0.23%
 39	   32864	  0.24%
 40	   36076	  0.26%
 41	   37040	  0.27%
 42	   39894	  0.29%
 43	   43446	  0.32%
 44	   46516	  0.34%
 45	   46698	  0.34%
 46	   47792	  0.35%
 47	   49202	  0.36%
 48	   50094	  0.36%
 49	   53992	  0.39%
 50	   57490	  0.42%
 51	   60601	  0.44%
 52	   65646	  0.48%
 53	   70192	  0.51%
 54	   72556	  0.53%
 55	   78421	  0.57%
 56	   90709	  0.66%
 57	   94647	  0.69%
 58	   94624	  0.69%
 59	   88455	  0.64%
 60	   88536	  0.64%
 61	   88542	  0.64%
 62	   91678	  0.66%
 63	   92020	  0.67%
 64	   95175	  0.69%
 65	   94366	  0.68%
 66	   99255	  0.72%
 67	  101425	  0.74%
 68	  103198	  0.75%
 69	  107751	  0.78%
 70	  111159	  0.81%
 71	  116529	  0.84%
 72	  117324	  0.85%
 73	  111319	  0.81%
 74	  111264	  0.81%
 75	  110632	  0.80%
 76	  112739	  0.82%
 77	  112668	  0.82%
 78	  118989	  0.86%
 79	  132460	  0.96%
 80	  164382	  1.19%
 81	  190906	  1.38%
 82	    1052	  0.01%
 83	   22926	  0.17%
 84	   89007	  0.65%
 85	  107563	  0.78%
 86	  109029	  0.79%
 87	  114271	  0.83%
 88	  114808	  0.83%
 89	  115134	  0.83%
 90	  112418	  0.82%
 91	  124312	  0.90%
 92	   76648	  0.56%
 93	   80283	  0.58%
 94	  108799	  0.79%
 95	  135972	  0.99%
 96	  163892	  1.19%
 97	  260850	  1.89%
 98	  402828	  2.92%
 99	  248949	  1.81%
100	 1088817	  7.89%
101	 6271796	 45.48%
13791660 reads passed initial QC


criterion=sequence-density
sequence-density=0.75
sequence-density-rank=1
fanout-score=4.02
fanout-score-rank=35
prefix-density=2.30
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGGAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=106.23
fanout-score-rank=1
prefix-density=0.21
prefix-fanout=3.1
sequence=TGTGTGTGGCAGTTTTAAGTTATTAGTTTTCAAAATCAGTACTTTTTAATGGAAACAACTTGACCAAAAATCTGTCACAGAATTTTGAGACCATTAAAACAAGTTTAATGA
                                 Started job on |	Jun 21 06:30:32
                             Started mapping on |	Jun 21 06:30:32
                                    Finished on |	Jun 21 06:30:44
       Mapping speed, Million of reads per hour |	4137.50

                          Number of input reads |	13791660
                      Average input read length |	88
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11825684
                        Uniquely mapped reads % |	85.75%
                          Average mapped length |	86.95
                       Number of splices: Total |	1126740
            Number of splices: Annotated (sjdb) |	1115341
                       Number of splices: GT/AG |	1119392
                       Number of splices: GC/AG |	3804
                       Number of splices: AT/AC |	408
               Number of splices: Non-canonical |	3136
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.47
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.26
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1853768
             % of reads mapped to multiple loci |	13.44%
        Number of reads mapped to too many loci |	64578
             % of reads mapped to too many loci |	0.47%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.34%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	112208	112208	112208
N_multimapping	1853768	1853768	1853768
N_noFeature	859713	896078	10816062
N_ambiguous	1112848	140158	615226
UnstrandedReadsAssigned:9853123 PositiveStrandReadsAssigned:10789448 NegativeStrandReadsAssigned:394396
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=79 echo kmer=75
SRR25194575 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194575-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,791,660 reads, 8,775,825 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,091 rounds

 109283 SRR25194575.ke.tsv
  52636 SRR25194575.se.tsv
 161919 total
==> SRR25194575.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194575.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194575 completed mapping pipeline successfully
