Starting /dee2/code/volunteer_pipeline.sh SRR25194569
    current disk space = 428927639552
    free memory = 391824320 
SRR25194569 SRAfilesize
b5f9edb027bd9c9608babcdd37673cc8  SRR25194569.sra
SRR25194569.sra file validated
SRR25194569 is single end
SRR25194569 is conventional basespace
SRR25194569 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0725	34.0	34.0	34.0	34.0	34.0
2	33.4805	34.0	34.0	34.0	34.0	34.0
3	33.391	34.0	34.0	34.0	34.0	34.0
4	33.495	34.0	34.0	34.0	34.0	34.0
5	33.6075	34.0	34.0	34.0	34.0	34.0
6	33.6275	34.0	34.0	34.0	34.0	34.0
7	33.5735	34.0	34.0	34.0	34.0	34.0
8	33.5145	34.0	34.0	34.0	34.0	34.0
9	33.6365	34.0	34.0	34.0	34.0	34.0
10-11	33.649249999999995	34.0	34.0	34.0	34.0	34.0
12-13	33.6285	34.0	34.0	34.0	34.0	34.0
14-15	33.615	34.0	34.0	34.0	34.0	34.0
16-17	33.5185	34.0	34.0	34.0	34.0	34.0
18-19	33.47475	34.0	34.0	34.0	34.0	34.0
20-21	33.560753132832076	34.0	34.0	34.0	34.0	34.0
22-23	33.53973324142233	34.0	34.0	34.0	34.0	34.0
24-25	33.53783953090084	34.0	34.0	34.0	34.0	34.0
26-27	33.6090613149332	34.0	34.0	34.0	34.0	34.0
28-29	33.6170566552623	34.0	34.0	34.0	34.0	34.0
30-31	33.569035699324445	34.0	34.0	34.0	34.0	34.0
32-33	33.61526983852551	34.0	34.0	34.0	34.0	34.0
34-35	33.594886344745035	34.0	34.0	34.0	34.0	34.0
36-37	33.63826828085898	34.0	34.0	34.0	34.0	34.0
38-39	33.60346024923928	34.0	34.0	34.0	34.0	34.0
40-41	33.57603526744758	34.0	34.0	34.0	34.0	34.0
42-43	33.571727337468964	34.0	34.0	34.0	34.0	34.0
44-45	33.583053377105635	34.0	34.0	34.0	34.0	34.0
46-47	33.55221754193675	34.0	34.0	34.0	34.0	34.0
48-49	33.538406049467824	34.0	34.0	34.0	34.0	34.0
50-51	33.54104563704661	34.0	34.0	34.0	34.0	34.0
52-53	33.572695757824874	34.0	34.0	34.0	34.0	34.0
54-55	33.59074493091032	34.0	34.0	34.0	34.0	34.0
56-57	33.54933758280215	34.0	34.0	34.0	34.0	34.0
58-59	33.471668809680764	34.0	34.0	34.0	34.0	34.0
60-61	33.532123705234426	34.0	34.0	34.0	34.0	34.0
62-63	33.48346018898398	34.0	34.0	34.0	34.0	34.0
64-65	33.48223857616326	34.0	34.0	34.0	34.0	34.0
66-67	33.347088096431534	34.0	34.0	34.0	34.0	34.0
68-69	33.35340517567522	34.0	34.0	34.0	34.0	34.0
70-71	33.29307057016195	34.0	34.0	34.0	34.0	34.0
72-73	33.34076545525913	34.0	34.0	34.0	34.0	34.0
74-75	33.33550839797731	34.0	34.0	34.0	34.0	34.0
76-77	33.18848575742517	34.0	34.0	34.0	34.0	34.0
78-79	33.24714328273801	34.0	34.0	34.0	34.0	34.0
80-81	33.256988885713696	34.0	34.0	34.0	34.0	34.0
82-83	33.319949281487744	34.0	34.0	34.0	34.0	34.0
84-85	33.22256950512667	34.0	34.0	34.0	34.0	34.0
86-87	33.16004907824288	34.0	34.0	34.0	34.0	34.0
88-89	33.170556926541025	34.0	34.0	34.0	34.0	34.0
90-91	33.17202098411289	34.0	34.0	34.0	34.0	34.0
92-93	33.03075335145502	34.0	34.0	34.0	34.0	34.0
94-95	33.02592669602957	34.0	34.0	34.0	34.0	34.0
96-97	33.01408459383754	34.0	34.0	34.0	34.0	34.0
98-99	33.0320482427547	34.0	34.0	34.0	34.0	34.0
100-101	32.82195907606893	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	2.0
26	4.0
27	15.0
28	19.0
29	46.0
30	58.0
31	94.0
32	230.0
33	2555.0
34	975.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.55	22.900000000000002	33.650000000000006	20.9
2	29.325000000000003	25.874999999999996	26.724999999999998	18.075
3	29.825000000000003	26.724999999999998	25.124999999999996	18.325
4	27.575	27.05	25.650000000000002	19.725
5	24.25	29.675	25.85	20.225
6	27.525	30.475	25.174999999999997	16.825000000000003
7	27.200000000000003	26.400000000000002	25.05	21.349999999999998
8	23.525	27.275	28.7	20.5
9	24.675	25.674999999999997	28.275	21.375
10-11	22.412499999999998	26.5875	30.337500000000002	20.6625
12-13	22.8875	32.125	28.037499999999998	16.950000000000003
14-15	21.637500000000003	34.225	30.049999999999997	14.0875
16-17	19.9125	32.0375	32.975	15.075
18-19	20.79779227295534	30.143000501756145	33.47967887606623	15.579528349222278
20-21	18.3729662077597	31.314142678347935	32.64080100125156	17.672090112640802
22-23	19.061362780775504	32.77701091730456	32.76446229137909	15.397164010540845
24-25	22.00150867488056	30.563238622076945	32.23535328136787	15.199899421674628
26-27	19.64757709251101	31.74323473882945	32.95154185022026	15.65764631843927
28-29	19.096871846619575	30.36074672048436	33.942986881937436	16.59939455095863
30-31	20.032861476238626	32.848837209302324	29.33518705763397	17.783114256825076
32-33	21.252853157494293	32.031448135937104	30.62389043875222	16.091808267816386
34-35	19.71741344195519	33.61761710794297	31.02087576374745	15.64409368635438
36-37	20.821602252367548	32.070642436652165	29.43434860506783	17.673406705912463
38-39	23.305849007987632	30.739500128832773	29.837670703426948	16.116980159752643
40-41	20.25365601138864	34.24356153746603	29.06690824382037	16.435874207324964
42-43	19.867463617463617	36.25259875259875	29.39189189189189	14.488045738045738
44-45	19.411764705882355	36.30065359477124	27.333333333333332	16.954248366013072
46-47	17.749145411517222	35.95845385222193	30.27872732053642	16.013673415724426
48-49	17.076085513212057	35.745584915681846	31.310582923914488	15.867746647191607
50-51	17.433544730805558	35.54176224531103	31.84455539063554	15.180137633247876
52-53	17.63331500824629	36.008796041781196	32.49037932930182	13.867509620670699
54-55	18.197041585263747	33.03097962601172	35.98939436226626	12.782584426458273
56-57	20.12987012987013	29.898362507058156	36.26482213438735	13.706945228684358
58-59	20.19423021993716	31.962296486718078	32.1622393601828	15.681233933161954
60-61	19.281140861466824	28.419674039580904	37.71827706635623	14.580908032596044
62-63	19.42117288651942	28.530083777608528	38.96420411271897	13.084539223153085
64-65	21.035957547916997	30.255029304609536	35.118010454617455	13.59100269285601
66-67	19.948270287746524	30.455868089233757	33.430326543808604	16.16553507921112
68-69	16.033825236279224	32.81379539048251	35.40043110595258	15.75194826728569
70-71	18.51916681253282	32.25975844565027	33.16996324172939	16.05111150008752
72-73	17.97074954296161	30.62157221206581	35.86837294332724	15.539305301645337
74-75	21.099190664408056	31.244118200639942	33.276868059476755	14.379823075475247
76-77	18.66769945778466	34.430673896204496	34.217660728117735	12.683965917893106
78-79	19.443892750744787	29.751737835153925	34.91559086395233	15.888778550148958
80-81	20.004103405826836	28.600738613048833	33.483791546984	17.911366434140337
82-83	16.990701606086223	32.94590025359256	34.40405748098056	15.659340659340659
84-85	16.12492033142129	32.483535160399406	35.500318674314855	15.891225833864459
86-87	20.10432514670724	30.99326233427516	32.68854596826777	16.213866550749838
88-89	17.39623497391699	33.54502154683602	30.36969834429576	18.689045134951236
90-91	16.327507780703854	31.912856116830262	32.60713430691884	19.152501795547046
92-93	16.522598172388246	33.61323783650284	32.40306248456409	17.461101506544825
94-95	18.044147843942504	32.75154004106776	31.878850102669404	17.32546201232033
96-97	17.438475787245302	34.13601481873512	31.013495633765547	17.412013760254037
98-99	20.676799541152853	30.28391167192429	33.09435044450817	15.944938342414684
100-101	8.363516628251565	35.89068159367797	36.77971682581495	18.966084952255517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	2.0
16	1.0
17	3.0
18	6.0
19	10.0
20	11.5
21	19.0
22	50.0
23	77.0
24	88.0
25	178.0
26	264.5
27	250.0
28	220.0
29	203.0
30	207.5
31	197.0
32	174.0
33	181.5
34	200.5
35	197.0
36	171.0
37	158.0
38	147.5
39	159.0
40	189.5
41	192.5
42	180.5
43	184.0
44	167.5
45	148.5
46	168.5
47	187.5
48	176.0
49	130.0
50	85.5
51	50.5
52	35.0
53	30.0
54	21.0
55	20.0
56	22.0
57	30.5
58	25.0
59	11.0
60	8.0
61	4.0
62	2.0
63	3.5
64	4.0
65	2.5
66	6.5
67	7.5
68	3.5
69	3.0
70	2.0
71	1.0
72	1.0
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.35000000000000003
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.34735623311462754
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	12.0
22-23	10.0
24-25	5.0
26-27	5.0
28-29	10.0
30-31	10.0
32-33	15.0
34-35	19.0
36-37	30.0
38-39	18.0
40-41	15.0
42-43	18.0
44-45	26.0
46-47	29.0
48-49	55.0
50-51	67.0
52-53	64.0
54-55	36.0
56-57	44.0
58-59	54.0
60-61	138.0
62-63	140.0
64-65	65.0
66-67	76.0
68-69	138.0
70-71	145.0
72-73	74.0
74-75	78.0
76-77	65.0
78-79	78.0
80-81	95.0
82-83	3.0
84-85	44.0
86-87	80.0
88-89	143.0
90-91	52.0
92-93	85.0
94-95	55.0
96-97	109.0
98-99	155.0
100-101	1640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.675000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.540354894104176	21.2
2	21.751574127074985	19.0
3	11.3909559244419	14.924999999999999
4	7.040641099026904	12.3
5	4.693760732684602	10.25
6	2.633085289066972	6.9
7	1.8317115054378934	5.6000000000000005
8	0.7441327990841442	2.6
9	0.40068689181453926	1.575
>10	0.9730967372638809	5.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	25	0.625	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	20	0.5	No Hit
CCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	17	0.42500000000000004	No Hit
TTCTCATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTG	14	0.35000000000000003	No Hit
AGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	14	0.35000000000000003	No Hit
CTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	14	0.35000000000000003	No Hit
TGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAA	13	0.325	No Hit
ACAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATC	12	0.3	No Hit
TGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATATT	12	0.3	No Hit
AAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	12	0.3	No Hit
GTCAAAGTCTTAAGCCAAGTAGAGAAAGCCCTAGATGCCCGTGTCTATTT	11	0.27499999999999997	No Hit
TCGCGGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAA	11	0.27499999999999997	No Hit
TCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATATAC	11	0.27499999999999997	No Hit
AATCTTCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTC	10	0.25	No Hit
TGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTC	10	0.25	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	10	0.25	No Hit
TTGTTGCAGCAAGAAAAGGATCAGAAGTTGTTAGAGATGGAAAAGATAAT	10	0.25	No Hit
GCTACTGGGTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	9	0.22499999999999998	No Hit
TTTGCGCGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATA	9	0.22499999999999998	No Hit
ACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAGATTTAT	9	0.22499999999999998	No Hit
TGGCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	9	0.22499999999999998	No Hit
TCACAACCACCTGTAATGAGATCTGACACCCACTTCTGGTGCGTCAGCTA	9	0.22499999999999998	No Hit
GGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGA	9	0.22499999999999998	No Hit
ATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAAT	9	0.22499999999999998	No Hit
TTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGG	8	0.2	No Hit
TGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAAT	8	0.2	No Hit
TCGATGCCAGCATCAGCAACAGAATACAAGAGATGGAAGAGAGAATCTCA	8	0.2	No Hit
CATGCACTAAGGCTGATCTTCAATAACAAAATAAATAATAGAAAGCCAAC	8	0.2	No Hit
GTGGGCAATCTAGTGGAGACCTTTTCTCAGTTGAGGTTTCTCTTACCAGA	8	0.2	No Hit
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	8	0.2	No Hit
AGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	8	0.2	No Hit
TGATGTTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATC	8	0.2	No Hit
ATGAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATATA	8	0.2	No Hit
CTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	8	0.2	No Hit
TAGTTGGGAGTCACTATGTAAGTGCTGGGTATTGAACCTGGATTCTATAA	8	0.2	No Hit
ACATCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACA	8	0.2	No Hit
CACCTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	8	0.2	No Hit
AACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	7	0.17500000000000002	No Hit
TGTCTGGTTTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACT	7	0.17500000000000002	No Hit
TCGACGTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	7	0.17500000000000002	No Hit
ATGGCAGCAGGTAGGTGTCTCTGGAAGCAGTGAGTAGAGTCACTGTTTTA	7	0.17500000000000002	No Hit
CTTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGT	7	0.17500000000000002	No Hit
TTGACCAGGTGCTAGAATCTCATGCCTCAACTATTCAGGAAAAAGAGCAA	7	0.17500000000000002	No Hit
TGGTGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTA	7	0.17500000000000002	No Hit
CACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	7	0.17500000000000002	No Hit
TAAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTTTTT	7	0.17500000000000002	No Hit
TGGGAGCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	7	0.17500000000000002	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
AAACAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAA	7	0.17500000000000002	No Hit
ATAGCAGAACACAGCAGAAGGAAATGCTGAAGTCTTTACTTGAACAAGAG	7	0.17500000000000002	No Hit
AAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCT	7	0.17500000000000002	No Hit
TATAGAAAGGAGCTTGTTGCTTATCTCACTTTGTTTTTAACTTTGCACTT	7	0.17500000000000002	No Hit
CCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGTA	7	0.17500000000000002	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
GAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGAGTC	7	0.17500000000000002	No Hit
CTGCACAGCCATCTGGGTGAAACACTTGAGTAGGACTTTAAGCAAGACTA	7	0.17500000000000002	No Hit
AGCTGAAGACAGATGATGTAAATAAATATTGGTGGCATACAGTGTTAAGTAGTGTGCAAGAGTGAAAGTT	7	0.17500000000000002	No Hit
CTCCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	7	0.17500000000000002	No Hit
CGAGGCATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAGATGGCATTA	7	0.17500000000000002	No Hit
CTTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TTGCATAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAA	7	0.17500000000000002	No Hit
TGTCTGGAGTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACT	7	0.17500000000000002	No Hit
TGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	7	0.17500000000000002	No Hit
TGGACTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	7	0.17500000000000002	No Hit
GAAACAGATGAGATAAAGATCTTGTTGGAAGAGAGCAGAACACAGCAGAA	7	0.17500000000000002	No Hit
TGTAATGGGGGCAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
CCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAAAAATAAA	7	0.17500000000000002	No Hit
CTTCAGGGTTGTTGTTAGCAGTAATAAATGATTCTTTTTCAGAAATATTT	7	0.17500000000000002	No Hit
TGTGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCA	7	0.17500000000000002	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	6	0.15	No Hit
AAGGAGCAGGTTGAGAAAGACCGGTTGATTCTGTTGAGAGAAATAGCAAT	6	0.15	No Hit
AGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	6	0.15	No Hit
AATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAA	6	0.15	No Hit
GCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	6	0.15	No Hit
TGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATGTTTCAT	6	0.15	No Hit
CAGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGG	6	0.15	No Hit
AAGCTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTT	6	0.15	No Hit
GTATGAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCAT	6	0.15	No Hit
GGACAGAATCTGAAGTAAGTTTCAAAGGGATTGATTAGAACCTAGGTCTG	6	0.15	No Hit
TGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAA	6	0.15	No Hit
CTCACAACCATCTGTAACAAGATCTGACGCCCTCTTCTGGAGTGTCTGAA	6	0.15	No Hit
AAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	6	0.15	No Hit
TGTTATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	6	0.15	No Hit
GTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTA	6	0.15	No Hit
TGAGGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTT	6	0.15	No Hit
GGGCAGAGTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGA	6	0.15	No Hit
CTGGATTCCGCAATGGAGACAAGCATGATGTCTGTCCAAGAAAACATGTT	6	0.15	No Hit
CATAAATTTACTTCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGA	6	0.15	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CATTCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACA	6	0.15	No Hit
TTGAGGGGGAAGAATTGTGGGAAGGAGTGACCAGGAAGGGAGCAATGAGC	6	0.15	No Hit
ATCCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	6	0.15	No Hit
TAATGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTC	6	0.15	No Hit
CGATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGT	6	0.15	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
AAAAAGCCATTGAGAAAAGACATCTGACCAGCATCTGGCCTCCATGTGTG	6	0.15	No Hit
ACACTACACTCTAGCTTGCTCGTTACATGCTTGGAGCTGCACAGCCATCT	6	0.15	No Hit
ATTAGACTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAA	6	0.15	No Hit
TTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTA	6	0.15	No Hit
ACCACGAGCAAGACAAAGAACACTTAATTCAGGAGCTTAATTTTGAAAAA	6	0.15	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TTCACAACCATCTGTAATGAAATCTGATGCCCTCTTCTGGAGTATCTGAG	6	0.15	No Hit
AAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGT	6	0.15	No Hit
ATTCTCAGCATCTTAGGACTGTTTCAAGTAATCATATTGATGAACTCGTA	6	0.15	No Hit
AGGCTGAGTCAGAAAGATGGCTTTAAGTTATTTGAGTTACTCAGTAAGTT	6	0.15	No Hit
TGGCAGCCAGAGAAGATTCTTCAAGCTTAGTTGCGGAACTTCAAGAGAAA	6	0.15	No Hit
CTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCA	6	0.15	No Hit
ACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	6	0.15	No Hit
GCTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGAAAATGAATATAATTAAAGGATTCTCACTC	6	0.15	No Hit
GGGAGAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CACCAGTCTCAGACCTCTTTTTAAAAGTTTTATTGAGGGGGAAGAATTGT	6	0.15	No Hit
TTCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTT	6	0.15	No Hit
GCAGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	6	0.15	No Hit
CGGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
AAAGTGCACCACTGCCAAACAAGAGCTAGCAAACAATCTCCACGTCAGAC	5	0.125	No Hit
TCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCC	5	0.125	No Hit
GCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTG	5	0.125	No Hit
GAGAATGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTT	5	0.125	No Hit
GGTAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACA	5	0.125	No Hit
AAGCGGGTAATACATGCCTTTCATCTCAGTAATTAGGAGGCGGAGTCGAA	5	0.125	No Hit
TTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATG	5	0.125	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	5	0.125	No Hit
TGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	5	0.125	No Hit
GTCGTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTC	5	0.125	No Hit
TGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTG	5	0.125	No Hit
TGCCGAAAACTTAAGAACAGAAATAAGTAAACTAAACCAAAAAATTCATG	5	0.125	No Hit
ATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACG	5	0.125	No Hit
TGAGGGTTTTGATGTAGCAGTTTTACTTTTTTTGTTGTTTGTTTGTTTTT	5	0.125	No Hit
TGGAATTGGGCAGAACACAGCAGAAGGAAATGCTGAAGTCTTTACTTGAA	5	0.125	No Hit
CTGGAATGGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
TGTGGCAGAAACATAGCACCCAAGTCTGAAACAATTGGCACCAGTCTTCC	5	0.125	No Hit
TAGCCAGAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCTTA	5	0.125	No Hit
TACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGC	5	0.125	No Hit
TTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGTGCATTT	5	0.125	No Hit
AGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTG	5	0.125	No Hit
TGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATG	5	0.125	No Hit
TTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	5	0.125	No Hit
AAATGGCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTT	5	0.125	No Hit
TCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAA	5	0.125	No Hit
CGACAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATT	5	0.125	No Hit
TACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GTGAAATTAAAGAACTGAAGCAGTCACGAGAGATGGCATTAGAAGACCTG	5	0.125	No Hit
TTGTAGCTGTGCAGATGTCGGAGCCCCGCCCCTCAATAAAGTTCTGTGACC	5	0.125	No Hit
ACCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	5	0.125	No Hit
AATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TTAGTTACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	5	0.125	No Hit
GCCAAAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCTTAGT	5	0.125	No Hit
TTACTTCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTC	5	0.125	No Hit
CTGTCCCCCCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTA	5	0.125	No Hit
GGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	5	0.125	No Hit
TGAGGCCAGTCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGAGCTACACAGAGAAACCCTGTCTCTAAAAACC	5	0.125	No Hit
ACTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACG	5	0.125	No Hit
GACGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	5	0.125	No Hit
AATGGCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTCAAAACACATACACATAGAC	5	0.125	No Hit
TGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTT	5	0.125	No Hit
TAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAAATTGTATAAAATTAG	5	0.125	No Hit
GACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	5	0.125	No Hit
TCGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTC	5	0.125	No Hit
GTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAAT	5	0.125	No Hit
CTGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	5	0.125	No Hit
TAACTCGTAATTCATGGTTGACCTTCAGAAGAAGATATTCATTGTATATT	5	0.125	No Hit
ATGCCTTGAGGGACTTGCTCCCCACAATCACTGTCCAGTTTTAGCCAGTT	5	0.125	No Hit
TTCTGAGCATCTTAGGACTGTTTCAAGTAATCATATTGATGAACTCGTAA	5	0.125	No Hit
TATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	5	0.125	No Hit
GTTATGGTGGTCAATGGAGGAGAATGCATGGCTGCAGATCGAAGAGTGTG	5	0.125	No Hit
AGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGG	5	0.125	No Hit
ATGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GAGTACCAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAA	5	0.125	No Hit
CGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTC	5	0.125	No Hit
ATAAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAG	5	0.125	No Hit
ATACTTCAGTTATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTC	5	0.125	No Hit
TATTAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	5	0.125	No Hit
CCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTT	5	0.125	No Hit
CGATCAGTCATGGAGACGGCAGATGAAGGAAGACTGGATTCCGCAATGGA	5	0.125	No Hit
CTTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	5	0.125	No Hit
CGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTGCTCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTTTTTT	5	0.125	No Hit
CTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	5	0.125	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	5	0.125	No Hit
GACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATC	5	0.125	No Hit
TGCAGGCCTTTCCAAGGGTCTTGAACCCGAGGAAAAGTTTAGGTCAGGTAAGAATACCTGGAGAGAGATT	5	0.125	No Hit
CGGAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TTAGAGACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	5	0.125	No Hit
GATCAATGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAAT	5	0.125	No Hit
GATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGG	5	0.125	No Hit
ATGTTGGTTCTGAGCAGTTCTTTGAGAGGGAACTCATTTATTCCTATCTCCAGTTTTAGCAC	5	0.125	No Hit
TGCGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CACGCGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATC	5	0.125	No Hit
GATCGCGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
ATCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGT	5	0.125	No Hit
TTCTGGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTT	5	0.125	No Hit
TACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
TGGGAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCC	5	0.125	No Hit
GAGGTCGAGGCAGGAGCATTTGAGCAACAGAGAATGACAACATCTGAAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791439 spots for SRR25194569.sra
Written 791439 spots for SRR25194569.sra
Read 791444 spots for SRR25194569.sra
Written 791444 spots for SRR25194569.sra
SRR ids: ['SRR25194569.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_lwzc0sfm
SRR25194569.sra spots: 12663029
blocks: [[1, 791439], [791440, 1582878], [1582879, 2374317], [2374318, 3165756], [3165757, 3957195], [3957196, 4748634], [4748635, 5540073], [5540074, 6331512], [6331513, 7122951], [7122952, 7914390], [7914391, 8705829], [8705830, 9497268], [9497269, 10288707], [10288708, 11080146], [11080147, 11871585], [11871586, 12663029]]
SRR25194569 file size 347413
SRR25194569 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25194569 SRR25194569_1.fastq
Input file:	SRR25194569_1.fastq
trimmed:	SRR25194569-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Sun Jun 21 10:58:17 2026 >> started

Sun Jun 21 10:58:25 2026 >> done (8.031s)
12663029 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
12663029 (100.00%) reads available; of these:
      81 ( 0.00%) trimmed reads available after processing
12662948 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    9540	  0.08%
 21	    8964	  0.07%
 22	    8853	  0.07%
 23	    9329	  0.07%
 24	   10045	  0.08%
 25	   11043	  0.09%
 26	   11445	  0.09%
 27	   12494	  0.10%
 28	   13984	  0.11%
 29	   16481	  0.13%
 30	   18792	  0.15%
 31	   22777	  0.18%
 32	   27016	  0.21%
 33	   27839	  0.22%
 34	   26873	  0.21%
 35	   27430	  0.22%
 36	   28917	  0.23%
 37	   30880	  0.24%
 38	   34008	  0.27%
 39	   37192	  0.29%
 40	   39961	  0.32%
 41	   41825	  0.33%
 42	   44774	  0.35%
 43	   50051	  0.40%
 44	   52227	  0.41%
 45	   51216	  0.40%
 46	   51838	  0.41%
 47	   53094	  0.42%
 48	   54012	  0.43%
 49	   57002	  0.45%
 50	   60124	  0.47%
 51	   63369	  0.50%
 52	   69784	  0.55%
 53	   73870	  0.58%
 54	   75335	  0.59%
 55	   80934	  0.64%
 56	  100961	  0.80%
 57	  101237	  0.80%
 58	   94577	  0.75%
 59	   88469	  0.70%
 60	   85839	  0.68%
 61	   84637	  0.67%
 62	   85125	  0.67%
 63	   85210	  0.67%
 64	   86129	  0.68%
 65	   86981	  0.69%
 66	   92819	  0.73%
 67	   94485	  0.75%
 68	   97155	  0.77%
 69	  102047	  0.81%
 70	  105538	  0.83%
 71	  108374	  0.86%
 72	  106950	  0.84%
 73	  100581	  0.79%
 74	  101274	  0.80%
 75	  102681	  0.81%
 76	  100001	  0.79%
 77	  101689	  0.80%
 78	  106446	  0.84%
 79	  118498	  0.94%
 80	  148084	  1.17%
 81	  171781	  1.36%
 82	     766	  0.01%
 83	   20772	  0.16%
 84	   77483	  0.61%
 85	   93935	  0.74%
 86	   96993	  0.77%
 87	  100278	  0.79%
 88	  102359	  0.81%
 89	  103123	  0.81%
 90	  102134	  0.81%
 91	  112271	  0.89%
 92	   67879	  0.54%
 93	   69671	  0.55%
 94	   97060	  0.77%
 95	  123801	  0.98%
 96	  147026	  1.16%
 97	  231858	  1.83%
 98	  356085	  2.81%
 99	  211426	  1.67%
100	  948091	  7.49%
101	 5629132	 44.45%
12663029 reads passed initial QC


criterion=sequence-density
sequence-density=0.67
sequence-density-rank=1
fanout-score=3.90
fanout-score-rank=31
prefix-density=2.02
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGGAC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=110.34
fanout-score-rank=1
prefix-density=0.15
prefix-fanout=5.6
sequence=AAACAAAAACAGCAACAACAAAAAAAACTGTGTCAGGTGTCTTTTGAGTTGGAGGCTGGGATGTCTGGTTCCTGGAACAGGAAGGAGGGAATGTCAACACTGAAGCCAAGAGCTTGCATTGATCACAGAAACAATAAAAGACAGATGGCAAATGAA
                                 Started job on |	Jun 21 10:58:41
                             Started mapping on |	Jun 21 10:58:42
                                    Finished on |	Jun 21 10:59:04
       Mapping speed, Million of reads per hour |	2072.13

                          Number of input reads |	12663029
                      Average input read length |	87
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10709635
                        Uniquely mapped reads % |	84.57%
                          Average mapped length |	85.93
                       Number of splices: Total |	883189
            Number of splices: Annotated (sjdb) |	872430
                       Number of splices: GT/AG |	875797
                       Number of splices: GC/AG |	3838
                       Number of splices: AT/AC |	415
               Number of splices: Non-canonical |	3139
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.39
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.19
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1823919
             % of reads mapped to multiple loci |	14.40%
        Number of reads mapped to too many loci |	72277
             % of reads mapped to too many loci |	0.57%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.44%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	129475	129475	129475
N_multimapping	1823919	1823919	1823919
N_noFeature	998384	1048290	9855916
N_ambiguous	909688	106242	498994
UnstrandedReadsAssigned:8801563 PositiveStrandReadsAssigned:9555103 NegativeStrandReadsAssigned:354725
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=79 echo kmer=75
SRR25194569 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194569-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 12,663,029 reads, 7,732,821 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,041 rounds

 109283 SRR25194569.ke.tsv
  52636 SRR25194569.se.tsv
 161919 total
==> SRR25194569.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194569.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194569 completed mapping pipeline successfully
