Starting /dee2/code/volunteer_pipeline.sh SRR25194568
    current disk space = 428899591168
    free memory = 392817040 
SRR25194568 SRAfilesize
a21a4c0a33fbbc96b9d930ec3679e038  SRR25194568.sra
SRR25194568.sra file validated
SRR25194568 is single end
SRR25194568 is conventional basespace
SRR25194568 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.25	34.0	34.0	34.0	34.0	34.0
2	33.5225	34.0	34.0	34.0	34.0	34.0
3	33.405	34.0	34.0	34.0	34.0	34.0
4	33.456	34.0	34.0	34.0	34.0	34.0
5	33.5945	34.0	34.0	34.0	34.0	34.0
6	33.579	34.0	34.0	34.0	34.0	34.0
7	33.5505	34.0	34.0	34.0	34.0	34.0
8	33.6355	34.0	34.0	34.0	34.0	34.0
9	33.5665	34.0	34.0	34.0	34.0	34.0
10-11	33.62075	34.0	34.0	34.0	34.0	34.0
12-13	33.626000000000005	34.0	34.0	34.0	34.0	34.0
14-15	33.64875000000001	34.0	34.0	34.0	34.0	34.0
16-17	33.61025	34.0	34.0	34.0	34.0	34.0
18-19	33.5115	34.0	34.0	34.0	34.0	34.0
20-21	33.56709913685263	34.0	34.0	34.0	34.0	34.0
22-23	33.53541927409262	34.0	34.0	34.0	34.0	34.0
24-25	33.56694156364318	34.0	34.0	34.0	34.0	34.0
26-27	33.600729803866784	34.0	34.0	34.0	34.0	34.0
28-29	33.61198492339011	34.0	34.0	34.0	34.0	34.0
30-31	33.618901899088186	34.0	34.0	34.0	34.0	34.0
32-33	33.62895315562935	34.0	34.0	34.0	34.0	34.0
34-35	33.60008974485166	34.0	34.0	34.0	34.0	34.0
36-37	33.64588684120356	34.0	34.0	34.0	34.0	34.0
38-39	33.597831334613645	34.0	34.0	34.0	34.0	34.0
40-41	33.57652359016372	34.0	34.0	34.0	34.0	34.0
42-43	33.57181762367055	34.0	34.0	34.0	34.0	34.0
44-45	33.53365266886722	34.0	34.0	34.0	34.0	34.0
46-47	33.493813963616205	34.0	34.0	34.0	34.0	34.0
48-49	33.497687159981474	34.0	34.0	34.0	34.0	34.0
50-51	33.47604948371013	34.0	34.0	34.0	34.0	34.0
52-53	33.544488370021284	34.0	34.0	34.0	34.0	34.0
54-55	33.49133918170709	34.0	34.0	34.0	34.0	34.0
56-57	33.51084157796548	34.0	34.0	34.0	34.0	34.0
58-59	33.44442841028982	34.0	34.0	34.0	34.0	34.0
60-61	33.49421801913917	34.0	34.0	34.0	34.0	34.0
62-63	33.52017950746759	34.0	34.0	34.0	34.0	34.0
64-65	33.44408818946019	34.0	34.0	34.0	34.0	34.0
66-67	33.42977274441806	34.0	34.0	34.0	34.0	34.0
68-69	33.34753908183775	34.0	34.0	34.0	34.0	34.0
70-71	33.23646619592566	34.0	34.0	34.0	34.0	34.0
72-73	33.211684022702535	34.0	34.0	34.0	34.0	34.0
74-75	33.33044323743236	34.0	34.0	34.0	34.0	34.0
76-77	33.10022862172228	34.0	34.0	34.0	34.0	34.0
78-79	33.24835005349274	34.0	34.0	34.0	34.0	34.0
80-81	33.20917861503874	34.0	34.0	34.0	34.0	34.0
82-83	33.307727690892364	34.0	34.0	34.0	34.0	34.0
84-85	33.11436819162748	34.0	34.0	34.0	34.0	34.0
86-87	33.104457748874	34.0	34.0	34.0	34.0	34.0
88-89	33.09281688813495	34.0	34.0	34.0	34.0	34.0
90-91	33.01370189038488	34.0	34.0	34.0	34.0	34.0
92-93	32.904174041881795	34.0	34.0	34.0	34.0	34.0
94-95	32.948197997757305	34.0	34.0	34.0	34.0	34.0
96-97	33.06890133700273	34.0	34.0	34.0	34.0	34.0
98-99	32.95548924314846	34.0	34.0	34.0	34.0	34.0
100-101	32.57774102148036	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	3.0
25	5.0
26	8.0
27	21.0
28	26.0
29	33.0
30	49.0
31	112.0
32	240.0
33	2461.0
34	1042.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.025	21.425	32.05	21.5
2	30.75	28.000000000000004	23.175	18.075
3	28.725	26.424999999999997	25.95	18.9
4	27.85	27.025	23.3	21.825
5	27.925	28.449999999999996	23.799999999999997	19.825
6	25.900000000000002	26.950000000000003	26.325	20.825
7	27.1	25.650000000000002	26.325	20.925
8	24.425	28.000000000000004	27.175	20.4
9	24.025	26.625	27.35	22.0
10-11	23.3875	26.625	29.225	20.7625
12-13	22.787499999999998	30.4875	29.175	17.549999999999997
14-15	22.625	32.05	30.349999999999998	14.975
16-17	22.675	31.7375	31.5375	14.05
18-19	20.39127163280662	30.850263355906698	33.358414848256835	15.400050163029846
20-21	22.145804676753784	30.561460547705387	31.98699512317119	15.305739652369638
22-23	19.336670838548184	32.25281602002503	32.15269086357947	16.257822277847307
24-25	22.191574724172515	30.478936810431296	31.745235707121367	15.584252758274825
26-27	20.215971873430437	30.52486187845304	32.25765946760422	17.001506780512305
28-29	18.759436336185203	31.328636134876696	31.882234524408652	18.02969300452944
30-31	19.371529530540133	31.08278647147905	30.71680969207471	18.82887430590611
32-33	21.64112954286438	34.63340509054071	26.98493098645055	16.74053438014436
34-35	18.303230730094125	33.85906893920122	28.96209615873823	18.87560417196642
36-37	20.39692701664533	33.597951344430214	29.987195902688864	16.017925736235593
38-39	23.159114315139032	31.73274974253347	30.3038105046344	14.8043254376931
40-41	21.223114796579427	33.49313293599378	28.95827934698108	16.325472920445712
42-43	19.231774235693756	36.46459367650902	28.625555265220797	15.67807682257643
44-45	18.19736842105263	34.276315789473685	29.93421052631579	17.592105263157894
46-47	19.662323850039883	36.00106354692901	29.061419835150225	15.275192767880883
48-49	16.864864864864863	34.770270270270274	31.310810810810814	17.054054054054056
50-51	16.264068075761735	33.85945649190228	34.21630524293165	15.660170189404338
52-53	17.026043125175022	36.376365163819656	31.657798935872307	14.939792775133014
54-55	18.977272727272727	31.874999999999996	33.90625	15.241477272727272
56-57	20.11859994214637	30.47439976858548	35.290714492334395	14.11628579693376
58-59	19.501466275659823	29.457478005865102	35.86510263929619	15.175953079178885
60-61	19.584392285842426	27.62744804903573	38.660487367319476	14.127672297802363
62-63	17.27456153965544	30.544777277665684	39.95033369548347	12.230327487195405
64-65	18.766318537859007	28.883812010443865	36.471932114882506	15.877937336814622
66-67	19.151453684922245	30.61189993238675	36.08857336037863	14.148073022312374
68-69	17.025026436376454	32.358124779696865	34.825519915403596	15.791328868523088
70-71	17.086264346538318	33.89485375786745	33.43206219918549	15.586819696408739
72-73	19.374640322271244	33.85766353347401	31.65164013044312	15.116056013811624
74-75	17.59368836291913	32.9585798816568	34.97041420118343	14.477317554240631
76-77	17.73297675375437	32.07159020777618	35.79510388808887	14.40032915038058
78-79	17.67314189189189	32.07347972972973	33.91047297297297	16.342905405405407
80-81	18.119771025979745	28.84191985909291	34.43416996917657	18.60413914575077
82-83	17.594296228150874	33.94664213431462	32.58969641214352	15.869365225390986
84-85	17.663421418636997	30.713954566527583	34.840055632823365	16.78256838201205
86-87	20.67065868263473	30.94610778443114	33.60479041916168	14.778443113772454
88-89	20.37593984962406	30.927318295739347	30.350877192982455	18.345864661654137
90-91	19.295958279009128	31.52542372881356	33.32464146023468	15.853976531942635
92-93	19.148936170212767	32.10583742498636	31.860338243316967	16.884888161483907
94-95	18.941979522184297	31.513083048919228	31.99658703071672	17.54835039817975
96-97	14.804722979109899	32.81864971238268	32.394792612776264	19.981834695731155
98-99	15.97199066355452	33.04434811603868	32.17739246415472	18.806268756252084
100-101	8.928571428571429	38.67781155015197	31.30699088145897	21.08662613981763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	4.5
21	27.5
22	49.5
23	55.5
24	88.5
25	176.5
26	279.0
27	294.0
28	235.5
29	180.0
30	176.5
31	170.5
32	147.5
33	184.0
34	228.0
35	222.0
36	180.5
37	175.5
38	189.5
39	186.5
40	190.0
41	178.5
42	168.5
43	189.0
44	170.5
45	124.0
46	149.5
47	180.5
48	156.5
49	125.5
50	112.0
51	87.5
52	55.5
53	44.0
54	38.5
55	41.0
56	37.5
57	32.5
58	28.5
59	20.0
60	12.5
61	6.5
62	4.5
63	2.5
64	1.0
65	0.0
66	0.0
67	0.0
68	1.0
69	1.0
70	0.0
71	0.0
72	0.0
73	1.5
74	1.5
75	1.0
76	1.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.325
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.47092547092547093
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	5.0
22-23	5.0
24-25	5.0
26-27	10.0
28-29	9.0
30-31	15.0
32-33	12.0
34-35	24.0
36-37	24.0
38-39	23.0
40-41	32.0
42-43	32.0
44-45	24.0
46-47	69.0
48-49	38.0
50-51	89.0
52-53	47.0
54-55	68.0
56-57	47.0
58-59	62.0
60-61	95.0
62-63	176.0
64-65	99.0
66-67	124.0
68-69	128.0
70-71	116.0
72-73	71.0
74-75	83.0
76-77	89.0
78-79	71.0
80-81	134.0
82-83	0.0
84-85	72.0
86-87	77.0
88-89	90.0
90-91	92.0
92-93	62.0
94-95	102.0
96-97	140.0
98-99	120.0
100-101	1419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.670363524337645	18.125
2	21.74984596426371	17.65
3	12.56931608133087	15.299999999999999
4	7.763401109057301	12.6
5	5.237215033887862	10.625
6	3.0191004313000613	7.35
7	1.478743068391867	4.2
8	1.1090573012939002	3.5999999999999996
9	0.7393715341959335	2.7
>10	1.6635859519408502	7.85
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	19	0.475	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	17	0.42500000000000004	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	15	0.375	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	14	0.35000000000000003	No Hit
CGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTT	13	0.325	No Hit
TGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTC	13	0.325	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	13	0.325	No Hit
ACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	13	0.325	No Hit
AGAATAGCATAGTTCATGAAGATAATCAATGGTTAATGTACTTTTACATG	12	0.3	No Hit
CTTCGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	12	0.3	No Hit
ATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	11	0.27499999999999997	No Hit
TTCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAA	11	0.27499999999999997	No Hit
CATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	11	0.27499999999999997	No Hit
GGTGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	10	0.25	No Hit
TTACACCTGAATTATATTTCTGTTGTCTCTTAGTTTTTAGAGAAATCAAC	10	0.25	No Hit
TAGCAGTTAGTTAAATAGTTAGCAGTTAGACTAATTTAGAACATATGACA	10	0.25	No Hit
GTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAA	10	0.25	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
AGCGGGCACGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAA	10	0.25	No Hit
TACTGTCAAGCCAAAAAGGCACAAAACAGATTTAAAGTTCCTTTGGGGAC	10	0.25	No Hit
TGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	10	0.25	No Hit
AGAGCTGAACACAGCAGAAGGAAATGCTGAAGTCTTTACTTGAACAAGAG	10	0.25	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
GAGTGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGAGTCA	10	0.25	No Hit
GGGAAGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTT	10	0.25	No Hit
TTGACCAGATGCTAGAATCTCATGCCTCAACTATTCAGGAAAAAGAGCAA	10	0.25	No Hit
TACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTT	10	0.25	No Hit
GTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	9	0.22499999999999998	No Hit
GGACGTGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTT	9	0.22499999999999998	No Hit
TGGTTAATGTACTTTTACATGTGCTAATTTTCTATTTGTAAACAGAAATT	9	0.22499999999999998	No Hit
TGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATG	9	0.22499999999999998	No Hit
TAAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCC	9	0.22499999999999998	No Hit
GAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	9	0.22499999999999998	No Hit
ACCTGCTTGCTCAGGGGTGGAGCTTCCTGCTCATTCGTTCTGCCACGCCC	9	0.22499999999999998	No Hit
ACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	9	0.22499999999999998	No Hit
TATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	9	0.22499999999999998	No Hit
GAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATC	9	0.22499999999999998	No Hit
GGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTT	9	0.22499999999999998	No Hit
TTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAG	9	0.22499999999999998	No Hit
ACTGTTGTGGGGGAGCACAGTCTAGCATTGTACCCTGTTTTAAACTTAGT	8	0.2	No Hit
AATGCATACTCAACATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAGA	8	0.2	No Hit
AAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAAC	8	0.2	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	8	0.2	No Hit
TAGACGAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACA	8	0.2	No Hit
TCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCAC	8	0.2	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	8	0.2	No Hit
TGTTCTTTCCTGTTTGTCTTTTCTTCCAAAAGTCTTGATCCCACTTCCCA	8	0.2	No Hit
TTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGT	8	0.2	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	8	0.2	No Hit
GAAAGACGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCACA	8	0.2	No Hit
TGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACT	8	0.2	No Hit
TGAGGAAAATTCATAGCTCTGAGTGCTGCCAAAATGAAGCTGGAGAGAGA	8	0.2	No Hit
TCGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGCC	8	0.2	No Hit
GAACAGCTGGCCATTGTTGGGTTTTTTTTTGTTGTTTGTTTGTTTGTTTG	8	0.2	No Hit
CAGCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	8	0.2	No Hit
GTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTG	8	0.2	No Hit
CCGCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	8	0.2	No Hit
GCTAGTTGCGGAACTTCAAGAGAAACTTCAAGAAGAAAAAGCTAAGTTTCTGGAACAACTTGAAGAACAA	7	0.17500000000000002	No Hit
CTCTGGCCTCCATGTGTGCCTGCATCTGGTCTCCATGTGTGCCTGCATCT	7	0.17500000000000002	No Hit
CACCGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAAC	7	0.17500000000000002	No Hit
ACCTGTCTTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGT	7	0.17500000000000002	No Hit
GATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	7	0.17500000000000002	No Hit
TTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAA	7	0.17500000000000002	No Hit
AGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	7	0.17500000000000002	No Hit
GGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTGCGCCTTCGGTTTAATTTATTTTT	7	0.17500000000000002	No Hit
CCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAG	7	0.17500000000000002	No Hit
GCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
ATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTT	7	0.17500000000000002	No Hit
TAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTTTTTT	7	0.17500000000000002	No Hit
CAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	7	0.17500000000000002	No Hit
TGACCCCCCCAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAA	7	0.17500000000000002	No Hit
CATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGCAGACT	7	0.17500000000000002	No Hit
TATGAGGAAATGCAAAATGTCAGAACCTCTTTGATTGCTGAGCAGCAGAC	7	0.17500000000000002	No Hit
TTGAGGGTTGTAAGAAGAAAAATGTTCATTAAACACTACAGAGAGTGGGC	7	0.17500000000000002	No Hit
TGAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	7	0.17500000000000002	No Hit
TGCCTCCCATCCATATCCAATCCTTTTTCTCCCAGTAGAAGGCTAACAGG	7	0.17500000000000002	No Hit
CAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	7	0.17500000000000002	No Hit
TTACAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGA	7	0.17500000000000002	No Hit
CTACAGTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	7	0.17500000000000002	No Hit
AAGCCAAAAACCAAAAACCAAACCAAACCAAACCAAAAATGCTCAACCCTCCCT	7	0.17500000000000002	No Hit
GGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCA	6	0.15	No Hit
GGCGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCT	6	0.15	No Hit
ATGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATG	6	0.15	No Hit
TGTGGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	6	0.15	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
ATGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	6	0.15	No Hit
TACTGTTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	6	0.15	No Hit
TGCTTAGAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAG	6	0.15	No Hit
CCAGCAGACAATGTAGCCAACAAAACAAAACAAAAC	6	0.15	No Hit
TATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATG	6	0.15	No Hit
CAGGGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCGGCAAGATAC	6	0.15	No Hit
CTGGCCTCCATGTGTGCCTGCATCTGCCCCCACATCCCATTTCTAAAGTAAGAGGGAAACTTTTGTTAT	6	0.15	No Hit
CACAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	6	0.15	No Hit
CACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
CTGGGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	6	0.15	No Hit
CAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATATACT	6	0.15	No Hit
AATCGTGAAACCACAGCTGTGCGTTTACAAATCTGAAGAATTTGGGTACA	6	0.15	No Hit
TATCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	6	0.15	No Hit
AGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CTTCCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGA	6	0.15	No Hit
AAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTG	6	0.15	No Hit
AGAGTGGTAGGATGAGGTTGGAGGATTACACAAAACTGTTAGGTAGGGCT	6	0.15	No Hit
TGCTGTTATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
CCGCAGCACAGGAGGCTGAGGCAGGAGCATTTGAGCAACAGAGAATGACA	6	0.15	No Hit
TTCACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACG	6	0.15	No Hit
TGTCTTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTC	6	0.15	No Hit
GGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	6	0.15	No Hit
TGTCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	6	0.15	No Hit
TGCGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	6	0.15	No Hit
ACCCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	6	0.15	No Hit
AATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCA	6	0.15	No Hit
GGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATT	6	0.15	No Hit
CGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAGAAAGGT	6	0.15	No Hit
GAGAGAGCTTGTTGAGAAAGAGTTATTAGAAAAAGTTAAACATCTTGAGA	6	0.15	No Hit
TATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	6	0.15	No Hit
TCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTG	6	0.15	No Hit
CGAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTT	6	0.15	No Hit
TGCCCTGGGTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	6	0.15	No Hit
TAATGAGGGTTACCAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAA	6	0.15	No Hit
CAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACC	6	0.15	No Hit
GGAATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	6	0.15	No Hit
ATTACGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	6	0.15	No Hit
CAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAA	6	0.15	No Hit
GCACGTGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGCCTTC	6	0.15	No Hit
CGCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGTTTGTAT	6	0.15	No Hit
TCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAAC	6	0.15	No Hit
GGGTTCTGAGCAGTTCTTTGAGAGGGAACTCATTTATTCCTATCTCCAGTTTTAGCACTAAAT	5	0.125	No Hit
CCGACAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAAT	5	0.125	No Hit
CTGTAATATGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAA	5	0.125	No Hit
AAGAAAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTT	5	0.125	No Hit
AGTCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTC	5	0.125	No Hit
GACCGAAACCCCGTCCCTTTTAAGGAGAATTATCCTCCGCCTAGGACGTA	5	0.125	No Hit
GCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	5	0.125	No Hit
TCAAGGCAAGACAAAGAACACTTAATTCAGGAGCTTAATTTTGAAAAAAA	5	0.125	No Hit
CCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTCAAAACA	5	0.125	No Hit
AAAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCA	5	0.125	No Hit
AAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCAT	5	0.125	No Hit
CCGAGCTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	5	0.125	No Hit
GCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
TGGCCATTGTTGGGTTTTTTTTTGTTGTTTGTTTGTTTGTTTGTTTTTAC	5	0.125	No Hit
CTGGTTTCATTGAAGAGAATATGTAACATATACCAATGTGTAAATCAGAT	5	0.125	No Hit
AGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTAT	5	0.125	No Hit
GGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGT	5	0.125	No Hit
ATTTTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAA	5	0.125	No Hit
TCTGAATTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	5	0.125	No Hit
TGAGGTCAGCCAGAGAAGATTCTTCAAGCTTAGTTGCGGAACTTCAAGAG	5	0.125	No Hit
GAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCT	5	0.125	No Hit
CTAGACAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	5	0.125	No Hit
CTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAAT	5	0.125	No Hit
TGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAAC	5	0.125	No Hit
CCCCTCAATAAAGTTCTGTGACC	5	0.125	No Hit
GATACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CTTGCTACTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	5	0.125	No Hit
TGGGAAAGTGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TCTGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTGCGCCTTCG	5	0.125	No Hit
TCAGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTT	5	0.125	No Hit
GCAGTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATATACTTAAG	5	0.125	No Hit
ATGGGTAAATTGAATTTAAATTTAAAATGTTAAATGCTGCCGGGCGTGTT	5	0.125	No Hit
CACACGTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	5	0.125	No Hit
CAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCAC	5	0.125	No Hit
AATTCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGGCC	5	0.125	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CAGTAGCTGGAAAGAGGAAGGAGACTAATGAGAGTATAAGGGAGAT	5	0.125	No Hit
TGACGGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	5	0.125	No Hit
GGCACATCACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
CGTAGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	5	0.125	No Hit
GCCAGAGAGATCAGTCATGGAGACGGCAGATGAAGGAAGACTGGATTCCG	5	0.125	No Hit
TCAAATGTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACC	5	0.125	No Hit
GCAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAG	5	0.125	No Hit
GATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGC	5	0.125	No Hit
GAAACCCTGTCTCAGCAAAAAAACAAAACAAAAACAAAACCAAAAAAAAAAAGAAAAAC	5	0.125	No Hit
CATTGACGTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTT	5	0.125	No Hit
TGTAAACTTGTCTGGAAGCTTCAGAGTTTTTGGACATTGTCTTCCAGTTG	5	0.125	No Hit
TCGTATTGGCCACTGGGATGTTTTTTAAGTTTTTATTTATTCAAATAAATTTACC	5	0.125	No Hit
GAGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
GCTGTTCAAAAATTTCGGATTTTCAGTCATCTCGACACCTGCCAAAAGAG	5	0.125	No Hit
GGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTTTTTTA	5	0.125	No Hit
GATAGATCCAGGCTCAGTGGCCAGCCAGTTTAGCTGCATGGTAGCTCACA	5	0.125	No Hit
CGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTT	5	0.125	No Hit
TGCTGAAATTTTTCTTACATGATGATGTCAAGCCAGCTGAAATGTAGGCT	5	0.125	No Hit
GTTTCCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATTGATAT	5	0.125	No Hit
ATGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CGTGTAGCTGTTATGGTTGGTGGAACAAACCATGCTCTTGGTGGGTCACT	5	0.125	No Hit
ATTCGTACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	5	0.125	No Hit
AAGACAGTGAGAAAAGACATCTGACCAGCATCTGGCCTCCATGTGTGCCT	5	0.125	No Hit
CTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGT	5	0.125	No Hit
GGGGAAACACGGCTTTTACTGTGAACTTTTAAAGAATGCTTATGTTACAT	5	0.125	No Hit
TGAGACCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTATTATT	5	0.125	No Hit
TCGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAA	5	0.125	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	5	0.125	No Hit
ACTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
TCCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	5	0.125	No Hit
ACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
CTTTGCACGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAG	5	0.125	No Hit
TGTGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCA	5	0.125	No Hit
CGGAAGTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
TATCTGGAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCT	5	0.125	No Hit
CATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATT	5	0.125	No Hit
CAAGAACCACGAGCAAGACAAAGAACACTTAATTCAGGAGCTTAATTTTG	5	0.125	No Hit
GATAAACGGCTTTTACTGTGAACTTTTAAAGAATGCTTATGTTACATATG	5	0.125	No Hit
GCGAGGCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGA	5	0.125	No Hit
GTTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTAT	5	0.125	No Hit
TCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACCAAT	5	0.125	No Hit
GGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	5	0.125	No Hit
TGAAGCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTT	5	0.125	No Hit
CTGTTCTGGCCTCCATGTGTGCCTGCATCTGCCCCCACATCCCATTTCTA	5	0.125	No Hit
TGCAGCAAGAAAAGGATCAGAAGTTGTTAGAGATGGAAAAGATAATGCAT	5	0.125	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATCGATTGCTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760128 spots for SRR25194568.sra
Written 760128 spots for SRR25194568.sra
Read 760139 spots for SRR25194568.sra
Written 760139 spots for SRR25194568.sra
SRR ids: ['SRR25194568.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_z0eahn4q
SRR25194568.sra spots: 12162059
blocks: [[1, 760128], [760129, 1520256], [1520257, 2280384], [2280385, 3040512], [3040513, 3800640], [3800641, 4560768], [4560769, 5320896], [5320897, 6081024], [6081025, 6841152], [6841153, 7601280], [7601281, 8361408], [8361409, 9121536], [9121537, 9881664], [9881665, 10641792], [10641793, 11401920], [11401921, 12162059]]
SRR25194568 file size 358075
SRR25194568 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25194568 SRR25194568_1.fastq
Input file:	SRR25194568_1.fastq
trimmed:	SRR25194568-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Sun Jun 21 11:43:43 2026 >> started

Sun Jun 21 11:43:53 2026 >> done (9.907s)
12162059 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
12162059 (100.00%) reads available; of these:
      68 ( 0.00%) trimmed reads available after processing
12161991 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    8452	  0.07%
 21	    8185	  0.07%
 22	    8222	  0.07%
 23	    8803	  0.07%
 24	    9566	  0.08%
 25	   10450	  0.09%
 26	   11270	  0.09%
 27	   12711	  0.10%
 28	   13777	  0.11%
 29	   15698	  0.13%
 30	   18842	  0.15%
 31	   22298	  0.18%
 32	   25922	  0.21%
 33	   26616	  0.22%
 34	   25912	  0.21%
 35	   26022	  0.21%
 36	   27883	  0.23%
 37	   29282	  0.24%
 38	   31885	  0.26%
 39	   34004	  0.28%
 40	   36952	  0.30%
 41	   37948	  0.31%
 42	   41521	  0.34%
 43	   46383	  0.38%
 44	   48841	  0.40%
 45	   47853	  0.39%
 46	   49311	  0.41%
 47	   50262	  0.41%
 48	   51251	  0.42%
 49	   53461	  0.44%
 50	   56308	  0.46%
 51	   59469	  0.49%
 52	   65178	  0.54%
 53	   70218	  0.58%
 54	   70704	  0.58%
 55	   77894	  0.64%
 56	   94633	  0.78%
 57	   95825	  0.79%
 58	   90614	  0.75%
 59	   85398	  0.70%
 60	   83453	  0.69%
 61	   82834	  0.68%
 62	   84022	  0.69%
 63	   85111	  0.70%
 64	   85992	  0.71%
 65	   85989	  0.71%
 66	   90572	  0.74%
 67	   92457	  0.76%
 68	   95737	  0.79%
 69	  100179	  0.82%
 70	  102930	  0.85%
 71	  106238	  0.87%
 72	  104658	  0.86%
 73	  100077	  0.82%
 74	   99054	  0.81%
 75	   99392	  0.82%
 76	   99238	  0.82%
 77	  101245	  0.83%
 78	  104052	  0.86%
 79	  115680	  0.95%
 80	  145001	  1.19%
 81	  168328	  1.38%
 82	     777	  0.01%
 83	   20816	  0.17%
 84	   78030	  0.64%
 85	   96165	  0.79%
 86	   98114	  0.81%
 87	  102194	  0.84%
 88	  103953	  0.85%
 89	  103790	  0.85%
 90	  101014	  0.83%
 91	  113854	  0.94%
 92	   66440	  0.55%
 93	   69071	  0.57%
 94	   98122	  0.81%
 95	  123840	  1.02%
 96	  144171	  1.19%
 97	  227290	  1.87%
 98	  349800	  2.88%
 99	  205968	  1.69%
100	  896603	  7.37%
101	 5323984	 43.78%
12162059 reads passed initial QC


criterion=sequence-density
sequence-density=0.59
sequence-density-rank=1
fanout-score=3.90
fanout-score-rank=28
prefix-density=1.76
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGGAC


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=20
fanout-score=42.93
fanout-score-rank=1
prefix-density=1.50
prefix-fanout=1.0
sequence=TTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAA
                                 Started job on |	Jun 21 11:44:13
                             Started mapping on |	Jun 21 11:44:14
                                    Finished on |	Jun 21 11:44:43
       Mapping speed, Million of reads per hour |	1509.77

                          Number of input reads |	12162059
                      Average input read length |	86
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10298776
                        Uniquely mapped reads % |	84.68%
                          Average mapped length |	85.79
                       Number of splices: Total |	863540
            Number of splices: Annotated (sjdb) |	853411
                       Number of splices: GT/AG |	856559
                       Number of splices: GC/AG |	3497
                       Number of splices: AT/AC |	435
               Number of splices: Non-canonical |	3049
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.44
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.19
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1743198
             % of reads mapped to multiple loci |	14.33%
        Number of reads mapped to too many loci |	66827
             % of reads mapped to too many loci |	0.55%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.42%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	120085	120085	120085
N_multimapping	1743198	1743198	1743198
N_noFeature	935484	978249	9398819
N_ambiguous	960896	104088	565283
UnstrandedReadsAssigned:8402396 PositiveStrandReadsAssigned:9216439 NegativeStrandReadsAssigned:334674
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=78 echo kmer=73
SRR25194568 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194568-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 12,162,059 reads, 7,339,322 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,181 rounds

 109283 SRR25194568.ke.tsv
  52636 SRR25194568.se.tsv
 161919 total
==> SRR25194568.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194568.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194568 completed mapping pipeline successfully
