Starting /dee2/code/volunteer_pipeline.sh SRR25194567
    current disk space = 1241737736
    free memory = 201074980 
SRR25194567 SRAfilesize
314dae8d9d1d7bab393eedd62c107cf9  SRR25194567.sra
SRR25194567.sra file validated
SRR25194567 is single end
SRR25194567 is conventional basespace
SRR25194567 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2855	34.0	34.0	34.0	34.0	34.0
2	33.439	34.0	34.0	34.0	34.0	34.0
3	33.438	34.0	34.0	34.0	34.0	34.0
4	33.517	34.0	34.0	34.0	34.0	34.0
5	33.573	34.0	34.0	34.0	34.0	34.0
6	33.6925	34.0	34.0	34.0	34.0	34.0
7	33.5455	34.0	34.0	34.0	34.0	34.0
8	33.5265	34.0	34.0	34.0	34.0	34.0
9	33.5965	34.0	34.0	34.0	34.0	34.0
10-11	33.6495	34.0	34.0	34.0	34.0	34.0
12-13	33.6205	34.0	34.0	34.0	34.0	34.0
14-15	33.637	34.0	34.0	34.0	34.0	34.0
16-17	33.6695	34.0	34.0	34.0	34.0	34.0
18-19	33.545249999999996	34.0	34.0	34.0	34.0	34.0
20-21	33.60679004004004	34.0	34.0	34.0	34.0	34.0
22-23	33.60450736239664	34.0	34.0	34.0	34.0	34.0
24-25	33.58204004584033	34.0	34.0	34.0	34.0	34.0
26-27	33.663778996445586	34.0	34.0	34.0	34.0	34.0
28-29	33.655825113488206	34.0	34.0	34.0	34.0	34.0
30-31	33.66774924389122	34.0	34.0	34.0	34.0	34.0
32-33	33.6569182303613	34.0	34.0	34.0	34.0	34.0
34-35	33.649521089652254	34.0	34.0	34.0	34.0	34.0
36-37	33.64131311092529	34.0	34.0	34.0	34.0	34.0
38-39	33.61664468462692	34.0	34.0	34.0	34.0	34.0
40-41	33.665665638029566	34.0	34.0	34.0	34.0	34.0
42-43	33.571663688655576	34.0	34.0	34.0	34.0	34.0
44-45	33.52414952278015	34.0	34.0	34.0	34.0	34.0
46-47	33.618567180420825	34.0	34.0	34.0	34.0	34.0
48-49	33.50419506160675	34.0	34.0	34.0	34.0	34.0
50-51	33.5510831626886	34.0	34.0	34.0	34.0	34.0
52-53	33.58453982349122	34.0	34.0	34.0	34.0	34.0
54-55	33.55211979483808	34.0	34.0	34.0	34.0	34.0
56-57	33.50972408085107	34.0	34.0	34.0	34.0	34.0
58-59	33.52647259255173	34.0	34.0	34.0	34.0	34.0
60-61	33.47018993872945	34.0	34.0	34.0	34.0	34.0
62-63	33.45531617860003	34.0	34.0	34.0	34.0	34.0
64-65	33.46949452753914	34.0	34.0	34.0	34.0	34.0
66-67	33.41357159736781	34.0	34.0	34.0	34.0	34.0
68-69	33.34596926067675	34.0	34.0	34.0	34.0	34.0
70-71	33.32122374331361	34.0	34.0	34.0	34.0	34.0
72-73	33.30091439600781	34.0	34.0	34.0	34.0	34.0
74-75	33.333205562851504	34.0	34.0	34.0	34.0	34.0
76-77	33.05759507027392	34.0	34.0	34.0	34.0	34.0
78-79	33.14362612082799	34.0	34.0	34.0	34.0	34.0
80-81	33.20033558226157	34.0	34.0	34.0	34.0	34.0
82-83	33.152602523659304	34.0	34.0	34.0	34.0	34.0
84-85	33.17945128530343	34.0	34.0	34.0	34.0	34.0
86-87	33.11482031804333	34.0	34.0	34.0	34.0	34.0
88-89	33.1362219490612	34.0	34.0	34.0	34.0	34.0
90-91	33.1881933247583	34.0	34.0	34.0	34.0	34.0
92-93	33.05957052377767	34.0	34.0	34.0	34.0	34.0
94-95	33.05167863307176	34.0	34.0	34.0	34.0	34.0
96-97	32.9917469748004	34.0	34.0	34.0	34.0	34.0
98-99	32.87277230197905	34.0	34.0	34.0	34.0	34.0
100-101	32.82529173332965	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	2.0
26	5.0
27	15.0
28	42.0
29	27.0
30	50.0
31	76.0
32	206.0
33	2736.0
34	840.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.175	20.525	30.15	21.15
2	27.05	26.674999999999997	25.6	20.674999999999997
3	32.0	24.9	24.975	18.125
4	28.749999999999996	28.1	24.0	19.15
5	27.375	30.925000000000004	24.075	17.625
6	29.325000000000003	28.875	23.125	18.675
7	26.825	26.875	26.974999999999998	19.325
8	25.6	25.874999999999996	28.499999999999996	20.025000000000002
9	24.975	24.325	26.85	23.849999999999998
10-11	22.0625	27.925	28.9125	21.099999999999998
12-13	23.25	29.875	29.762499999999996	17.1125
14-15	22.35	32.35	29.725	15.575
16-17	23.05	30.4375	31.162499999999998	15.35
18-19	20.682730923694777	31.664156626506024	31.990461847389557	15.66265060240964
20-21	20.847923961980992	30.702851425712858	32.666333166583286	15.78289144572286
22-23	19.411396368190356	31.74702567313713	31.52160300563557	17.319974953036944
24-25	19.969894631209232	31.71098845960863	32.12493728048169	16.19417962870045
26-27	21.48148148148148	30.947897049591965	31.060891399874453	16.509730069052104
28-29	20.788845622409244	31.604069840472306	30.812711970857933	16.79437256626052
30-31	22.107645875251507	30.596076458752513	29.86670020120724	17.429577464788732
32-33	20.418452230904965	32.84597932946811	29.3042601462062	17.43130829342072
34-35	20.138976626658245	32.01516108654454	29.349336702463674	18.496525584333547
36-37	20.693583090748007	34.185546133400834	26.920642956587777	18.200227819263386
38-39	22.35189648610935	33.08385132563745	27.9715844221743	16.592667766078904
40-41	22.084873199949026	33.018988148336945	29.004715177774948	15.891423473939085
42-43	20.570916538658473	36.16231438812084	28.21300563236047	15.053763440860216
44-45	20.172613680278243	32.538966894241916	29.202627850057965	18.085791575421872
46-47	18.464730290456433	36.60529045643153	27.100622406639	17.82935684647303
48-49	17.60397593512948	35.456447815851426	30.90504839131572	16.034527857703374
50-51	17.929025423728813	33.659957627118644	31.289724576271187	17.121292372881356
52-53	18.24570887957832	35.47776726584674	32.35572374645223	13.92080010812272
54-55	17.862511958452917	32.239989066557335	34.57701243679103	15.320486538198717
56-57	17.800829875518673	32.15767634854772	33.3609958506224	16.6804979253112
58-59	19.665038381018842	29.867411025819955	34.34752267969296	16.12002791346825
60-61	19.50596252129472	27.69733106189665	36.99602498580352	15.800681431005112
62-63	19.53888807821392	32.52590106522691	34.42288049029622	13.512330366262951
64-65	19.09706546275395	30.158013544018058	34.82317531978932	15.921745673438675
66-67	19.553846153846155	30.353846153846153	34.64615384615385	15.446153846153846
68-69	18.50561975621339	33.73436757954725	33.54440398923539	14.215608675003958
70-71	17.315238718116415	32.47220405493787	34.1890124264225	16.02354480052322
72-73	18.112889637742207	31.30581297388374	33.142375737152484	17.438921651221566
74-75	19.019368347583317	32.437619961612285	32.769150235560986	15.773861455243413
76-77	18.162083936324166	31.928364688856732	34.04486251808972	15.864688856729378
78-79	18.09399962845997	33.53148801783392	32.2868289058146	16.08768344789151
80-81	18.155729864166826	31.815572986416683	32.1025444805816	17.926152668834895
82-83	17.547318611987382	31.56545741324921	34.22712933753943	16.660094637223974
84-85	15.803108808290157	31.845356715823037	35.13351933041052	17.218015145476283
86-87	18.794688457609805	30.56179775280899	32.03268641470889	18.61082737487232
88-89	18.48421052631579	30.757894736842108	31.621052631578948	19.136842105263156
90-91	18.89661164205039	29.930495221546483	31.950477845351866	19.22241529105126
92-93	17.151294865970012	30.395274875056792	34.302589731940024	18.150840527033164
94-95	17.236500825277055	32.56307474652205	33.90709738269276	16.293327045508136
96-97	17.102665350444223	34.79763079960513	30.256663376110566	17.84304047384008
98-99	17.948031074203055	31.931422448432894	31.04741494776319	19.073131529600857
100-101	8.734567901234568	36.882716049382715	34.074074074074076	20.308641975308642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.5
15	0.5
16	0.5
17	2.0
18	9.0
19	13.5
20	9.5
21	18.0
22	43.0
23	74.0
24	112.5
25	147.0
26	213.5
27	235.5
28	200.5
29	181.5
30	165.0
31	153.0
32	149.5
33	144.5
34	131.0
35	154.0
36	162.0
37	167.0
38	178.0
39	169.0
40	171.5
41	179.5
42	205.5
43	200.5
44	164.5
45	146.0
46	148.5
47	167.0
48	169.5
49	126.0
50	77.0
51	72.5
52	67.5
53	51.0
54	34.5
55	29.5
56	31.5
57	20.5
58	19.0
59	19.5
60	15.0
61	17.0
62	15.0
63	7.5
64	2.5
65	0.0
66	0.0
67	0.0
68	0.0
69	1.0
70	3.0
71	2.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.3963963963963964
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	6.0
22-23	6.0
24-25	5.0
26-27	1.0
28-29	4.0
30-31	10.0
32-33	9.0
34-35	8.0
36-37	6.0
38-39	17.0
40-41	19.0
42-43	22.0
44-45	22.0
46-47	35.0
48-49	32.0
50-51	85.0
52-53	46.0
54-55	45.0
56-57	24.0
58-59	54.0
60-61	91.0
62-63	109.0
64-65	76.0
66-67	82.0
68-69	99.0
70-71	98.0
72-73	91.0
74-75	103.0
76-77	83.0
78-79	77.0
80-81	99.0
82-83	12.0
84-85	54.0
86-87	73.0
88-89	85.0
90-91	92.0
92-93	75.0
94-95	97.0
96-97	121.0
98-99	166.0
100-101	1761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.12087912087912	22.35
2	23.076923076923077	21.0
3	11.043956043956044	15.075
4	6.318681318681318	11.5
5	4.725274725274725	10.75
6	2.417582417582418	6.6000000000000005
7	1.3186813186813187	4.2
8	0.7142857142857143	2.6
9	0.43956043956043955	1.7999999999999998
>10	0.8241758241758242	4.125
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAA	13	0.325	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	13	0.325	No Hit
CCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	13	0.325	No Hit
GTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAAT	12	0.3	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	11	0.27499999999999997	No Hit
AAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTC	11	0.27499999999999997	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	11	0.27499999999999997	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	11	0.27499999999999997	No Hit
ATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACG	10	0.25	No Hit
TCTGAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTG	10	0.25	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
GCGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	10	0.25	No Hit
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	10	0.25	No Hit
GATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATAT	10	0.25	No Hit
GCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAATTAATACTTA	10	0.25	No Hit
AAAATGTTCTCTAGAAATAACACTAAAGGAAAAGCATCAGCAAGAACTCC	9	0.22499999999999998	No Hit
TAAGGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAA	9	0.22499999999999998	No Hit
GCCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCA	9	0.22499999999999998	No Hit
CAGGATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	9	0.22499999999999998	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
CTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	9	0.22499999999999998	No Hit
GAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
GTCTTTGCCTCTTATCTTTGGACAGTCTTTCCTAAAATGATTTATACTTA	9	0.22499999999999998	No Hit
TTGCTCCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGT	8	0.2	No Hit
TTGGAGGATTACACAAAACTGTTAGGTAGGGCTTTAGAGTGTTGTCAAGT	8	0.2	No Hit
GTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	8	0.2	No Hit
GTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	8	0.2	No Hit
AGGCATGATAGACTTTATATTGTTTTTAGCAGGAGCTAAAAAATACATTT	8	0.2	No Hit
GGTCCCAGCACTTGGGAGGTAGAGGCAGGTGGATTTTTGAGTTCGAGGCC	8	0.2	No Hit
GACAATGTTACATCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACT	8	0.2	No Hit
CACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAAT	8	0.2	No Hit
TTGCTGGCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGC	8	0.2	No Hit
TGAAAGGATGAATATAATCAAAATATATTGTGTGAAATTCTCAAAAACTA	8	0.2	No Hit
CTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCA	8	0.2	No Hit
GATCATGCATATCCATAAGTTTTCTGTCACAAGCTATTTTTCCTCATCCT	8	0.2	No Hit
CATCTTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	8	0.2	No Hit
TGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAAT	7	0.17500000000000002	No Hit
TGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCA	7	0.17500000000000002	No Hit
CATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAAC	7	0.17500000000000002	No Hit
GCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCAC	7	0.17500000000000002	No Hit
CTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGAT	7	0.17500000000000002	No Hit
GCAGGACTCACTCAAACCTCTCAGCATCTTAGGACTGTTTCAAGTAATCA	7	0.17500000000000002	No Hit
TACTGCCTGTATTAGAGAAATATTACTGTTATTTATATTCTTTATGATTT	7	0.17500000000000002	No Hit
TGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTC	7	0.17500000000000002	No Hit
AGTTGAACAGAGAATTAATACTTACGTATTTGTGGGTAATCAGCTTATCC	7	0.17500000000000002	No Hit
CCAAAAACCAAAAACCAAAAACCAAAAACCAAAAACCAAAAACCAAAAAC	7	0.17500000000000002	No Hit
TGGTTGACCTTCAGAAGAAGATATTCATTGTATATTAACATTTAGAGGTC	7	0.17500000000000002	No Hit
GTGGGGCTCCCTCCATGTGCTGTCAGGAATCTCCAAGACTTGGCTCGCAT	7	0.17500000000000002	No Hit
TTATTCTGTCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTG	7	0.17500000000000002	No Hit
GTATATAAACCCCTACCCATGCTCTTAAAGTAACCCCAGTTAAACCTTAAAAAGAAAGATCAGCATGAGAGT	7	0.17500000000000002	No Hit
CCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAATTAATACTTACG	7	0.17500000000000002	No Hit
ATGGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	7	0.17500000000000002	No Hit
TGGACTCTTAATAATTGGTGGACAATTGTTTTACAAATGTGGTTTTTGGT	7	0.17500000000000002	No Hit
CGCAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCG	7	0.17500000000000002	No Hit
GTCAGCTACTGTAAGTGATGGTTTAATTTTTATTCATTTCTAATTTTAAA	7	0.17500000000000002	No Hit
GCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	7	0.17500000000000002	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	7	0.17500000000000002	No Hit
TCGCAACCATCCATAACAAAATCTGATGCCCTCTTCTGGAGTATCTGAGG	7	0.17500000000000002	No Hit
TATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	7	0.17500000000000002	No Hit
GCATGATAGACTTTATATTGTTTTTAGCAGGAGCTAAAAAATACATTTTG	7	0.17500000000000002	No Hit
TCGTGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGA	6	0.15	No Hit
ATGCACCACCGGACTCTGTGGGGCTCCCTCCATGTGCTGTCAGGAATCTC	6	0.15	No Hit
TGGAAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAAT	6	0.15	No Hit
CGGTGTATGCAGAGATTGAAAAGATTTAGCAAAATCACGTGTGTTCTTTT	6	0.15	No Hit
ATGATGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAG	6	0.15	No Hit
GCGGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	6	0.15	No Hit
ATCACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
GAATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTT	6	0.15	No Hit
TTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATA	6	0.15	No Hit
CGGAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACA	6	0.15	No Hit
TCTAAGTTGCTTATACTCCTGTATATAAACCCCTACCCATGCTCTTAAAG	6	0.15	No Hit
TTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGAAA	6	0.15	No Hit
GGATGGAAAAGATAATGCATACTCAACATTGTGAAATTAAAGAACTGAAG	6	0.15	No Hit
TTGGTTGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAA	6	0.15	No Hit
GCAGCCCGTGGAGAGCATAGCAAACTGGAGTCCAGAGAAAGTGGGTAGAG	6	0.15	No Hit
TGAGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
TAGCAAAAACCTGAAAGACTCAAATGTCACAAGTGGGCCAGATGCCATAG	6	0.15	No Hit
ACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	6	0.15	No Hit
GGGAAGCATTAAATTGTTGACAAAATAACTCAAATGCTTGGCTGTAATAG	6	0.15	No Hit
GTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTT	6	0.15	No Hit
CTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTG	6	0.15	No Hit
CCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGA	6	0.15	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	6	0.15	No Hit
CTGTGATAAAGAATATGATCAAACTTGGCAGTGGTGGGTAATACATGCCT	6	0.15	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	6	0.15	No Hit
AGAGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGA	6	0.15	No Hit
TGAATGAAGCCAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACT	6	0.15	No Hit
CTTAGAACAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGC	6	0.15	No Hit
GCATCGGCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTG	6	0.15	No Hit
TACTCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTG	6	0.15	No Hit
TGGGAGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	6	0.15	No Hit
GAGGCGGAGTCGAATGGGTCTCTAAGTTGGCCAGGACAACAGAGAAACCC	6	0.15	No Hit
GTCGGGCGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGT	6	0.15	No Hit
ACTGTCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	6	0.15	No Hit
TGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTTCAAAC	6	0.15	No Hit
TCCTAGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTT	6	0.15	No Hit
GGGACAGGGGGCTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCT	6	0.15	No Hit
ACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGAT	6	0.15	No Hit
TATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCT	6	0.15	No Hit
ATGTAGTACACAATGAACATAAAAATAATAAAACCAAATCTAATTTTAAA	6	0.15	No Hit
GTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCAT	6	0.15	No Hit
TTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATT	6	0.15	No Hit
CCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAAC	6	0.15	No Hit
GGGAGGGTATGGGAGACTTTTGGGATAGCATTGGAAATGTAAATGAAGAA	6	0.15	No Hit
TCAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAAG	5	0.125	No Hit
AAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAA	5	0.125	No Hit
CTTAGGGCCAGCTAATATCTTCAACAAAATAATAGAAGAAAACTTCCCAA	5	0.125	No Hit
TGAAAGCCCTAGATGCCCGTGTCTATTTTGTATTGGCCACTGGGATGTTT	5	0.125	No Hit
TGGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CGACCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTC	5	0.125	No Hit
CGGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GCACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAATT	5	0.125	No Hit
TCCGGACCTCTTTTTAAAAGTTTTATTGAGGGGGAAGAATTGTGGGAAGG	5	0.125	No Hit
CCACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTTGGAGAAATT	5	0.125	No Hit
GTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGA	5	0.125	No Hit
GATGACGTGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCC	5	0.125	No Hit
TGGAGGCAGAGGCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACA	5	0.125	No Hit
CCGTTACATCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAA	5	0.125	No Hit
GCCTGCATCTGGCCTCCATGTGTGCCTGCATCTGCCCCCACATCCCATTT	5	0.125	No Hit
CAGTGGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACAATG	5	0.125	No Hit
TTCAGTGGTTGTTGTTAGCAGTAATAAATGATTCTTTTTCAGAAATATTT	5	0.125	No Hit
CCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGC	5	0.125	No Hit
TGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAG	5	0.125	No Hit
GGTATAAGGAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCA	5	0.125	No Hit
TGCTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATTG	5	0.125	No Hit
GAGGTGTGGGTTTGAAATTTTGGATAAGGTGTCTTATTGTCTTGAGAATT	5	0.125	No Hit
ATTGCAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACG	5	0.125	No Hit
CTTGTGAGCTTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTG	5	0.125	No Hit
GAAATGATGAGATGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAG	5	0.125	No Hit
GAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
CTGGACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	5	0.125	No Hit
TTACGGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGG	5	0.125	No Hit
GTTGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
AGACAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	5	0.125	No Hit
AATGAGGAGGAAGAGATTTTAAACAAAAAACGATCAAAGAAAATTC	5	0.125	No Hit
TACGGAATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGA	5	0.125	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
CGGGTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	5	0.125	No Hit
ACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTCTTCTG	5	0.125	No Hit
AGAGAGTTCCAGCATCACAGGAGGCTGAGGCAGGAGCATTTGAGCAACAG	5	0.125	No Hit
TCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCACC	5	0.125	No Hit
CGATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTG	5	0.125	No Hit
TCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGG	5	0.125	No Hit
GTCGAAGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATT	5	0.125	No Hit
TTCTGTTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTG	5	0.125	No Hit
ATCACAAATAAGTATACAGGCCTTTCCAAGGGTCTTGAACCCGAGGAAAA	5	0.125	No Hit
CATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	5	0.125	No Hit
CTCGTGATGCGGGGGCTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGC	5	0.125	No Hit
TTATGCAGGCCTTTCCAAGGGTCTTGAACCCGAGGAAAAGTTAAGGTTAGGTAGGAATACCTGGAGAGAGATTA	5	0.125	No Hit
TTGTGTAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCT	5	0.125	No Hit
GCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGCTCCAGGA	5	0.125	No Hit
TGAAGATCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGAT	5	0.125	No Hit
GAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTT	5	0.125	No Hit
ACCGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACTAGCATATTA	5	0.125	No Hit
TAATGGTACACCGGACTCTGTGGGGCTCCCTCCATGTGCTGTCAGGAATC	5	0.125	No Hit
CCTGGGCGGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAG	5	0.125	No Hit
GCGAATCAAATAGCCAAAACGTAAGTATTAAGTTTAGAATGTAATTTACT	5	0.125	No Hit
CTGGGACGGGGCTTCAGGGACTGAGCGGGCGCTGCCGCTGTGTGAGCGTC	5	0.125	No Hit
GGGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	5	0.125	No Hit
CATCTTGACTCCATGTGTGCCTGCATCTGGTCTCCATGTGTGCCTGCATC	5	0.125	No Hit
GCAGAGGCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTG	5	0.125	No Hit
CTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATTGAT	5	0.125	No Hit
ATGTATTGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTT	5	0.125	No Hit
TGGACGAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACA	5	0.125	No Hit
AATTGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGAT	5	0.125	No Hit
GCTCCAGCAATCAAAACTCTTAAAATACAAAGGGATACTTTTTTCTTATT	5	0.125	No Hit
AACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCC	5	0.125	No Hit
TTGGAAGAGAGCAGAACACAGCAGAAGGAAATGCTGAAGTCTTTACTTGA	5	0.125	No Hit
CAAACCCTAATCAAATCACAAACAGAGGAGTTCATTTGGAAAAGCAATAC	5	0.125	No Hit
TTCGGCGTACACTTAGAAACTTCATAAATGATGAGATGCAGGCCAAGGGG	5	0.125	No Hit
AGCTGGGGATCTGTGTGATGGCACGTGAGAAGACTTTAAGACCACGAGGT	5	0.125	No Hit
TCGAAACCAGGGCCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5	0.125	No Hit
TTTTCGGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGT	5	0.125	No Hit
GAGCTTTATGACAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTA	5	0.125	No Hit
CAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCT	5	0.125	No Hit
TGTATTGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAA	5	0.125	No Hit
ACTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTC	5	0.125	No Hit
TAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTT	5	0.125	No Hit
ATGTACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	5	0.125	No Hit
TCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATG	5	0.125	No Hit
TCAGGGGAAGGCGGATTTCTGACTTTGAGGCCAGCCTGGTCTACATAGTG	5	0.125	No Hit
CACATGCCTGTTTCGTAGGCCTCTAGAGTTAGTGTCTCTTCTGATCCTTA	5	0.125	No Hit
GATGGGCTCGTAATTCATGGTTGACCTTCAGAAGAAGATATTCATTGTAT	5	0.125	No Hit
CAGCAGAAAATGCCAGGATTCATTTACTAGTCATTATCAAAAGATAGTTGCCTCATTTATTAGCTAGC	5	0.125	No Hit
GAATGAGGAAATGCAAAATGTCAGAACCTCTTTGATTGCTGAGCAGCAGA	5	0.125	No Hit
TGTGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGAA	5	0.125	No Hit
GAACATAAATTTACTTCAGTTATTCTATCTGTAAAGATTTATTTGGTATA	5	0.125	No Hit
CCTGAAGTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.025	0.0	0.0	0.0	0.0
86-87	0.025	0.0	0.0	0.0	0.0
88-89	0.0875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517090 spots for SRR25194567.sra
Written 1517090 spots for SRR25194567.sra
Read 1517093 spots for SRR25194567.sra
Written 1517093 spots for SRR25194567.sra
SRR ids: ['SRR25194567.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_01uqn3o3
SRR25194567.sra spots: 12136723
blocks: [[1, 1517090], [1517091, 3034180], [3034181, 4551270], [4551271, 6068360], [6068361, 7585450], [7585451, 9102540], [9102541, 10619630], [10619631, 12136723]]
SRR25194567 file size 2647692
SRR25194567 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25194567 SRR25194567_1.fastq
Input file:	SRR25194567_1.fastq
trimmed:	SRR25194567-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Jun 21 07:17:13 2026 >> started

Sun Jun 21 07:17:17 2026 >> done (3.867s)
12136723 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
12136723 (100.00%) reads available; of these:
      92 ( 0.00%) trimmed reads available after processing
12136631 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    5134	  0.04%
 21	    4842	  0.04%
 22	    4904	  0.04%
 23	    4992	  0.04%
 24	    5792	  0.05%
 25	    6345	  0.05%
 26	    6650	  0.05%
 27	    7688	  0.06%
 28	    8724	  0.07%
 29	    9983	  0.08%
 30	   11927	  0.10%
 31	   14432	  0.12%
 32	   16197	  0.13%
 33	   17126	  0.14%
 34	   16687	  0.14%
 35	   16942	  0.14%
 36	   18412	  0.15%
 37	   19485	  0.16%
 38	   21391	  0.18%
 39	   23353	  0.19%
 40	   25439	  0.21%
 41	   26305	  0.22%
 42	   28664	  0.24%
 43	   31465	  0.26%
 44	   33752	  0.28%
 45	   34001	  0.28%
 46	   35063	  0.29%
 47	   36905	  0.30%
 48	   37448	  0.31%
 49	   40471	  0.33%
 50	   42810	  0.35%
 51	   45734	  0.38%
 52	   50419	  0.42%
 53	   54525	  0.45%
 54	   54754	  0.45%
 55	   61083	  0.50%
 56	   75017	  0.62%
 57	   75817	  0.62%
 58	   72048	  0.59%
 59	   67786	  0.56%
 60	   67662	  0.56%
 61	   66469	  0.55%
 62	   67971	  0.56%
 63	   68357	  0.56%
 64	   70492	  0.58%
 65	   70928	  0.58%
 66	   75979	  0.63%
 67	   78021	  0.64%
 68	   80298	  0.66%
 69	   85169	  0.70%
 70	   87949	  0.72%
 71	   90752	  0.75%
 72	   90676	  0.75%
 73	   88292	  0.73%
 74	   88371	  0.73%
 75	   88437	  0.73%
 76	   87784	  0.72%
 77	   89149	  0.73%
 78	   92837	  0.76%
 79	  104670	  0.86%
 80	  131434	  1.08%
 81	  151542	  1.25%
 82	     693	  0.01%
 83	   18551	  0.15%
 84	   68948	  0.57%
 85	   85048	  0.70%
 86	   88862	  0.73%
 87	   93052	  0.77%
 88	   94281	  0.78%
 89	   97338	  0.80%
 90	   94703	  0.78%
 91	  108459	  0.89%
 92	   63110	  0.52%
 93	   64912	  0.53%
 94	   93715	  0.77%
 95	  117711	  0.97%
 96	  143955	  1.19%
 97	  229156	  1.89%
 98	  360127	  2.97%
 99	  235608	  1.94%
100	 1017077	  8.38%
101	 5967666	 49.17%
12136723 reads passed initial QC


criterion=sequence-density
sequence-density=0.70
sequence-density-rank=1
fanout-score=3.82
fanout-score-rank=31
prefix-density=2.09
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGGAC


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=21
fanout-score=48.59
fanout-score-rank=1
prefix-density=1.74
prefix-fanout=1.0
sequence=TTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAA
                                 Started job on |	Jun 21 07:17:22
                             Started mapping on |	Jun 21 07:17:22
                                    Finished on |	Jun 21 07:17:33
       Mapping speed, Million of reads per hour |	3972.02

                          Number of input reads |	12136723
                      Average input read length |	89
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10337149
                        Uniquely mapped reads % |	85.17%
                          Average mapped length |	88.57
                       Number of splices: Total |	950585
            Number of splices: Annotated (sjdb) |	939703
                       Number of splices: GT/AG |	943198
                       Number of splices: GC/AG |	3936
                       Number of splices: AT/AC |	358
               Number of splices: Non-canonical |	3093
                      Mismatch rate per base, % |	0.41%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.40
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.17
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1698080
             % of reads mapped to multiple loci |	13.99%
        Number of reads mapped to too many loci |	61660
             % of reads mapped to too many loci |	0.51%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.32%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	101494	101494	101494
N_multimapping	1698080	1698080	1698080
N_noFeature	934404	980580	9568065
N_ambiguous	837165	114584	424257
UnstrandedReadsAssigned:8565580 PositiveStrandReadsAssigned:9241985 NegativeStrandReadsAssigned:344827
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=85 echo kmer=81
SRR25194567 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194567-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 12,136,723 reads, 7,930,957 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,063 rounds

 109283 SRR25194567.ke.tsv
  52636 SRR25194567.se.tsv
 161919 total
==> SRR25194567.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194567.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194567 completed mapping pipeline successfully
