Starting /dee2/code/volunteer_pipeline.sh SRR25194572
    current disk space = 428889177088
    free memory = 392819912 
SRR25194572 SRAfilesize
9c2cb64e6555921c7238aa51a1645afd  SRR25194572.sra
SRR25194572.sra file validated
SRR25194572 is single end
SRR25194572 is conventional basespace
SRR25194572 read1 length is 20-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25194572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	20-101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.218	34.0	34.0	34.0	34.0	34.0
2	33.5445	34.0	34.0	34.0	34.0	34.0
3	33.446	34.0	34.0	34.0	34.0	34.0
4	33.4055	34.0	34.0	34.0	34.0	34.0
5	33.5975	34.0	34.0	34.0	34.0	34.0
6	33.624	34.0	34.0	34.0	34.0	34.0
7	33.6685	34.0	34.0	34.0	34.0	34.0
8	33.556	34.0	34.0	34.0	34.0	34.0
9	33.6485	34.0	34.0	34.0	34.0	34.0
10-11	33.685	34.0	34.0	34.0	34.0	34.0
12-13	33.65325	34.0	34.0	34.0	34.0	34.0
14-15	33.614000000000004	34.0	34.0	34.0	34.0	34.0
16-17	33.61775	34.0	34.0	34.0	34.0	34.0
18-19	33.5355	34.0	34.0	34.0	34.0	34.0
20-21	33.59088506753377	34.0	34.0	34.0	34.0	34.0
22-23	33.6257192894671	34.0	34.0	34.0	34.0	34.0
24-25	33.57848545636911	34.0	34.0	34.0	34.0	34.0
26-27	33.62597429639408	34.0	34.0	34.0	34.0	34.0
28-29	33.67095192018086	34.0	34.0	34.0	34.0	34.0
30-31	33.62888015246915	34.0	34.0	34.0	34.0	34.0
32-33	33.62402902541463	34.0	34.0	34.0	34.0	34.0
34-35	33.59732866000398	34.0	34.0	34.0	34.0	34.0
36-37	33.63879974205788	34.0	34.0	34.0	34.0	34.0
38-39	33.60920707136357	34.0	34.0	34.0	34.0	34.0
40-41	33.61520670755767	34.0	34.0	34.0	34.0	34.0
42-43	33.59060273973334	34.0	34.0	34.0	34.0	34.0
44-45	33.540056584695904	34.0	34.0	34.0	34.0	34.0
46-47	33.59507050657428	34.0	34.0	34.0	34.0	34.0
48-49	33.58703879181459	34.0	34.0	34.0	34.0	34.0
50-51	33.61529685344616	34.0	34.0	34.0	34.0	34.0
52-53	33.50340958371548	34.0	34.0	34.0	34.0	34.0
54-55	33.60767765066671	34.0	34.0	34.0	34.0	34.0
56-57	33.57155711852185	34.0	34.0	34.0	34.0	34.0
58-59	33.52246796077387	34.0	34.0	34.0	34.0	34.0
60-61	33.494500503727586	34.0	34.0	34.0	34.0	34.0
62-63	33.50824598867081	34.0	34.0	34.0	34.0	34.0
64-65	33.48363588751948	34.0	34.0	34.0	34.0	34.0
66-67	33.43336989262852	34.0	34.0	34.0	34.0	34.0
68-69	33.29166808844066	34.0	34.0	34.0	34.0	34.0
70-71	33.408153950620836	34.0	34.0	34.0	34.0	34.0
72-73	33.40603788531287	34.0	34.0	34.0	34.0	34.0
74-75	33.35225691292841	34.0	34.0	34.0	34.0	34.0
76-77	33.18489405130843	34.0	34.0	34.0	34.0	34.0
78-79	33.30071225356516	34.0	34.0	34.0	34.0	34.0
80-81	33.31444002242169	34.0	34.0	34.0	34.0	34.0
82-83	33.17900288807859	34.0	34.0	34.0	34.0	34.0
84-85	33.26501747885622	34.0	34.0	34.0	34.0	34.0
86-87	33.28120246556588	34.0	34.0	34.0	34.0	34.0
88-89	33.24452466685332	34.0	34.0	34.0	34.0	34.0
90-91	33.132298647125666	34.0	34.0	34.0	34.0	34.0
92-93	33.04281320870546	34.0	34.0	34.0	34.0	34.0
94-95	33.216663158870645	34.0	34.0	34.0	34.0	34.0
96-97	33.08896600373505	34.0	34.0	34.0	34.0	34.0
98-99	33.142429687712706	34.0	34.0	34.0	34.0	34.0
100-101	32.796913334190094	34.0	34.0	34.0	30.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	3.0
24	0.0
25	2.0
26	9.0
27	14.0
28	19.0
29	36.0
30	52.0
31	79.0
32	222.0
33	2552.0
34	1012.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.199999999999996	18.75	31.924999999999997	21.125
2	29.599999999999998	24.95	26.05	19.400000000000002
3	30.9	25.424999999999997	26.775	16.900000000000002
4	27.575	26.900000000000002	26.35	19.175
5	23.05	31.900000000000002	25.7	19.35
6	26.3	30.45	25.275	17.974999999999998
7	23.75	27.474999999999998	28.375	20.4
8	22.925	27.275	29.825000000000003	19.975
9	21.2	27.800000000000004	29.599999999999998	21.4
10-11	25.0375	23.1625	31.075000000000003	20.724999999999998
12-13	23.1625	30.7375	29.9	16.2
14-15	21.9	34.25	30.112499999999997	13.737499999999999
16-17	20.325	32.05	32.5375	15.0875
18-19	19.1921726041144	33.29152032112393	33.416959357752134	14.099347717009532
20-21	19.72993248312078	34.3710927731933	30.045011252813204	15.853963490872719
22-23	19.314485864398296	32.98724043032274	30.660495371528647	17.03777833375031
24-25	21.70260782347041	31.093279839518555	32.911233701103306	14.292878635907725
26-27	20.09545340366742	30.231097714142173	33.89851796031148	15.774930921878925
28-29	18.0251572327044	31.257861635220124	33.35849056603774	17.358490566037734
30-31	20.975056689342402	32.17435122197027	28.2312925170068	18.619299571680525
32-33	20.290771175726928	33.13527180783818	31.3527180783818	15.221238938053098
34-35	19.37404967055246	31.424227065382667	31.804358844399395	17.397364419665486
36-37	20.14489069649212	32.600406710727	29.969496695475346	17.28520589730554
38-39	21.363926067558957	29.91714467813894	32.87444231994901	15.84448693435309
40-41	21.14695340501792	31.976446492575523	29.7363031233999	17.140296979006656
42-43	18.615165999224907	35.667226456530166	30.719545278387805	14.998062265857124
44-45	18.237358637722608	33.22500974912258	31.678148966593007	16.859482646561812
46-47	19.448456992777412	34.60275771503611	30.76822061720289	15.180564674983584
48-49	18.360047688435554	34.85229831765797	30.10994833752815	16.67770565637833
50-51	17.543859649122805	34.498459890183476	33.23958751841435	14.718092942279362
52-53	17.18474277182028	36.25627799647075	32.64558164788924	13.913397583819737
54-55	17.779300891021247	32.2960932145305	36.16175462645648	13.762851267991774
56-57	19.30843706777317	29.75103734439834	36.56984785615491	14.370677731673581
58-59	20.058749475451112	30.899426493215838	34.49433487201007	14.547489159322982
60-61	16.479772888573457	31.156848828956708	38.154719659332855	14.208658623136976
62-63	17.904314646316408	29.057822130906956	38.6410331670091	14.396830055767538
64-65	19.103194103194106	28.1019656019656	35.810810810810814	16.984029484029485
66-67	18.200408997955012	30.84788422211735	32.84568192543652	18.106024854491114
68-69	16.01492779490508	33.00340743144572	34.98296284277138	15.99870193087782
70-71	16.486486486486488	30.945945945945947	34.7972972972973	17.77027027027027
72-73	18.973998594518623	31.482782853127194	31.939564300773014	17.603654251581165
74-75	18.40028694404591	32.53228120516499	33.50071736011478	15.566714490674318
76-77	21.653832049871653	32.43491015768243	31.004767143381006	14.906490649064907
78-79	17.900428864441544	30.468021629684877	35.85679656908447	15.77475293678911
80-81	16.605874448070647	30.946438855826454	35.05471299673642	17.392973699366483
82-83	18.020355218519256	35.04290560766314	31.96966673318699	14.967072440630613
84-85	19.62673088500903	32.37005819787277	32.79149106963676	15.211719847481437
86-87	17.173377156943303	36.38044371405095	30.279375513557927	16.166803615447822
88-89	19.647033808207524	31.93706145013821	31.150329576865833	17.265575164788434
90-91	18.892076804237476	32.68594129331274	31.207239020083865	17.214742882365925
92-93	17.195402298850574	33.54022988505747	33.01149425287356	16.25287356321839
94-95	17.88141551441294	30.255448793063046	34.66135458167331	17.201781110850714
96-97	16.59811275102831	33.77691749334624	29.7846600532301	19.840309702395352
98-99	15.749674054758799	31.23859191655802	35.01955671447197	17.992177314211215
100-101	8.71132472213878	32.201862421147496	37.098227696004805	21.988585160708922
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	2.0
16	1.5
17	2.5
18	2.5
19	1.5
20	6.0
21	23.0
22	41.5
23	74.5
24	106.0
25	191.5
26	310.5
27	300.0
28	235.5
29	204.0
30	196.0
31	179.5
32	153.5
33	135.5
34	114.0
35	134.5
36	142.0
37	124.5
38	139.5
39	164.5
40	173.5
41	184.0
42	201.5
43	191.0
44	176.0
45	174.5
46	182.0
47	185.0
48	165.0
49	117.5
50	77.5
51	50.0
52	29.5
53	28.5
54	32.5
55	38.0
56	25.5
57	9.5
58	5.0
59	11.0
60	15.0
61	6.5
62	4.0
63	5.0
64	1.5
65	1.0
66	1.0
67	1.0
68	1.0
69	0.5
70	2.5
71	2.5
72	0.5
73	4.5
74	4.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.35000000000000003
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.3835616438356164
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-21	3.0
22-23	9.0
24-25	6.0
26-27	5.0
28-29	6.0
30-31	13.0
32-33	10.0
34-35	10.0
36-37	13.0
38-39	15.0
40-41	33.0
42-43	24.0
44-45	35.0
46-47	38.0
48-49	37.0
50-51	44.0
52-53	44.0
54-55	26.0
56-57	47.0
58-59	37.0
60-61	99.0
62-63	165.0
64-65	79.0
66-67	99.0
68-69	97.0
70-71	142.0
72-73	61.0
74-75	56.0
76-77	48.0
78-79	68.0
80-81	125.0
82-83	6.0
84-85	47.0
86-87	74.0
88-89	96.0
90-91	95.0
92-93	47.0
94-95	50.0
96-97	111.0
98-99	189.0
100-101	1791.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.004513217279175	16.675
2	20.76079948420374	16.1
3	12.959381044487428	15.075
4	7.930367504835589	12.3
5	6.189555125725339	12.0
6	3.7395228884590583	8.7
7	1.6763378465506125	4.55
8	1.4184397163120568	4.3999999999999995
9	0.7092198581560284	2.475
>10	1.6118633139909737	7.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	24	0.6	No Hit
CTGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTT	20	0.5	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	18	0.44999999999999996	No Hit
TGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAA	15	0.375	No Hit
GCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	15	0.375	No Hit
GGTACGACATCAGTGCTGATGACCTTATGGAAACTGTATCATGGAGTCCT	13	0.325	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	13	0.325	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	12	0.3	No Hit
GAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGG	12	0.3	No Hit
ACGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	12	0.3	No Hit
ATGCAGGTGTCCTTGTTATATGTTGGAGAATGACACATCAAAACGACCAT	11	0.27499999999999997	No Hit
GTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAA	11	0.27499999999999997	No Hit
GCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGT	11	0.27499999999999997	No Hit
TATCAGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACA	11	0.27499999999999997	No Hit
GGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	11	0.27499999999999997	No Hit
CAGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	10	0.25	No Hit
CGATTAAACATGAAAAGGATGCTATTGTCTGTGTGCAGCAAGAAAAGGAT	10	0.25	No Hit
TTGCAAGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTT	10	0.25	No Hit
CAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACA	10	0.25	No Hit
TTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAA	10	0.25	No Hit
TCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGCT	10	0.25	No Hit
CTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	10	0.25	No Hit
TGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	10	0.25	No Hit
GTGTTGTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGA	10	0.25	No Hit
TGAAAAAAAGAAAATCAGCAAAGAATTGACCAGATGCTAGAATCTCATGC	10	0.25	No Hit
TGGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCT	9	0.22499999999999998	No Hit
TGCATGTTAAATTGTATAAAATTAGATACTGCCTGTATTAGAGAAATATT	9	0.22499999999999998	No Hit
CAGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACAATG	9	0.22499999999999998	No Hit
TTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTC	9	0.22499999999999998	No Hit
TAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	9	0.22499999999999998	No Hit
GGGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
CTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	9	0.22499999999999998	No Hit
TGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	9	0.22499999999999998	No Hit
CTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATTTTT	9	0.22499999999999998	No Hit
GATACTGCATGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGAT	9	0.22499999999999998	No Hit
CGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATT	9	0.22499999999999998	No Hit
AGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	8	0.2	No Hit
ATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACA	8	0.2	No Hit
CTAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTC	8	0.2	No Hit
GATGTCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCT	8	0.2	No Hit
TTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAA	8	0.2	No Hit
AACGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAGTG	8	0.2	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	8	0.2	No Hit
TTTTGATTTGCTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTA	8	0.2	No Hit
TGGTTGCAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTT	8	0.2	No Hit
TGCTAGATCCTTGGACTTCCATTCACAGCTACTACTGAACCATTGTTGGG	8	0.2	No Hit
GTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	8	0.2	No Hit
CGAGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTA	8	0.2	No Hit
GGGGCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGC	8	0.2	No Hit
CTGACCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAG	8	0.2	No Hit
GGTTGCCGACCCGACCTGAGGGCCAGAAGCAGTGTTACACACTTCCTGGA	8	0.2	No Hit
ATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAA	8	0.2	No Hit
CTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAATTGAT	8	0.2	No Hit
TTGAATGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTA	8	0.2	No Hit
GGCTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	8	0.2	No Hit
ATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	8	0.2	No Hit
GGTTGGTCTTTGTTGTTCACCTGACAGTCTGTCTTAAATGCCTTCTGTGG	8	0.2	No Hit
CGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGGTTTGAAACTTTTT	8	0.2	No Hit
GGGAGGTTCAGGTGCATCTAGAAGACCCTGGGTCCTTGGGAAAGTAATGG	7	0.17500000000000002	No Hit
AGAAAAAAGTGTTTGCTTGTTAAATTGTATAAAATTAGATACTGCCTGTA	7	0.17500000000000002	No Hit
AGATTGAACTTGCACTAAAGGAAGCAGAAACAGATGAGATAAAGATCTTG	7	0.17500000000000002	No Hit
GTTTGAGAGTTACCAGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAA	7	0.17500000000000002	No Hit
GTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAAT	7	0.17500000000000002	No Hit
TGAGAATCAAATAGCCAAAACGTAAGTATTAAGTTTAGAATGTAATTTAC	7	0.17500000000000002	No Hit
ACAGCCAGAGAAGATTCTTCAAGCTTAGTTGCGGAACTTCAAGAGAAACT	7	0.17500000000000002	No Hit
CAGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	7	0.17500000000000002	No Hit
CCTTAGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCA	7	0.17500000000000002	No Hit
TTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGT	7	0.17500000000000002	No Hit
CTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAG	7	0.17500000000000002	No Hit
CATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	7	0.17500000000000002	No Hit
CTAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTAT	7	0.17500000000000002	No Hit
CAGCCTGAAACCTGCTTGCTCAGGGGTGGAGCTTCCTGCTCATTCGTTCT	7	0.17500000000000002	No Hit
CGAGCAAGACAAAGAACACTTAATTCAGGAGCTTAATTTTGAAAAAAACA	7	0.17500000000000002	No Hit
GTCCGACGGTAGAATGAAGCCAAATGTCTGGATTTAAAACAATTTCAAAC	7	0.17500000000000002	No Hit
GTGTAGCCCCCTGGGGGAGGAGAAAGAAAACAACTAGCTCAAGAGCCTAG	7	0.17500000000000002	No Hit
GGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGA	7	0.17500000000000002	No Hit
GAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATG	7	0.17500000000000002	No Hit
GGAGGAGTCTGTGCCACTGATGTTTTCAAGTGCAGGACTCACTCAAACCT	7	0.17500000000000002	No Hit
TGGTGGGTTTGTCAGAGTTAAGAGCTTTAATGACAATTGAAAAAGATCAG	7	0.17500000000000002	No Hit
GCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	7	0.17500000000000002	No Hit
AAGTAGCAGGCCTTTCCAAGGGTCTTGAACCCGAGGAAAAGTTTAGGTCA	7	0.17500000000000002	No Hit
CTCGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGG	7	0.17500000000000002	No Hit
TTTTGGGCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	7	0.17500000000000002	No Hit
TCGGGGAGGCAGAGGCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCT	6	0.15	No Hit
AACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGA	6	0.15	No Hit
GCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAG	6	0.15	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	6	0.15	No Hit
GCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATCTGTAAAGA	6	0.15	No Hit
TCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAG	6	0.15	No Hit
CGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAGG	6	0.15	No Hit
GATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAG	6	0.15	No Hit
CCTAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	6	0.15	No Hit
GGACAGGGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCG	6	0.15	No Hit
GCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTG	6	0.15	No Hit
TGAAAAAGCTGCATGATGAAAAAATCGAGTCATTGAGAGCTGAATTTCAG	6	0.15	No Hit
TTGAAAAAGTTAAACATCTTGAGAATCAAATAGCCAAAACTCCTGCCTTT	6	0.15	No Hit
ACACCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGC	6	0.15	No Hit
TGTTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAA	6	0.15	No Hit
CAACCACATGGTGGCTCACAACCATCTGTAACAAGATCTGACGCCCTCTT	6	0.15	No Hit
TGTTGTATAGCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTG	6	0.15	No Hit
GCCGTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTC	6	0.15	No Hit
GCCTTTTCCCTATGGTAATTCCTGGGAGAATAAAAGTGTTTGCTTGTTAA	6	0.15	No Hit
ATGACCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAG	6	0.15	No Hit
AAAAACTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCT	6	0.15	No Hit
TGGACTAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTG	6	0.15	No Hit
GCGCCTGACTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	6	0.15	No Hit
GAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTTTTT	6	0.15	No Hit
TGCGCAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TCTGGCCAGCATCTGGCCTCCATGTGTGCCTGCATCTGGTCTCCATGTGT	6	0.15	No Hit
TACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCAT	6	0.15	No Hit
TACTGGCCTCCATGTGTGCCTGCATCTGGTCTCCATGTGTGCCTGCATCT	6	0.15	No Hit
TGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTC	6	0.15	No Hit
AGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGA	6	0.15	No Hit
ACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
TAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	6	0.15	No Hit
CTGTAAGGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TTGTTTGTATCCTATGTAACAGGGTGAAGTGGTGTTCTGTGGAACAGAAC	6	0.15	No Hit
TAGTTAGCAGTTAGACTAATTTAGAACATATGACATAATGAGTTTGAAGA	6	0.15	No Hit
CCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	6	0.15	No Hit
GGCTGAATTTCAGTGCTTAGAACAAAATCACCTGAAGGAATTAGAGGACA	6	0.15	No Hit
CCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGT	6	0.15	No Hit
TGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCT	6	0.15	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
CTTCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACA	6	0.15	No Hit
ATAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAG	6	0.15	No Hit
ATTTTGGGCATGAAAAGAACCCTGGAAAGTGTGGGCTATTTTGTTGGTGA	6	0.15	No Hit
AAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	6	0.15	No Hit
TCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTG	6	0.15	No Hit
AACAACATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACA	6	0.15	No Hit
GATTTGGTTGACCTTCAGAAGAAGATATTCATTGTATATTAACATTTAGA	6	0.15	No Hit
GAGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TTCCCCCCCAAAAAAAAAGATTGGTCCATGGATAATTTCTGTCACTTTAA	6	0.15	No Hit
CTTTGGCATGCAATTTATTCTCTGCCTCAGTTTCACCATCTGCCAAATGGAGGGTAAAATAAAAATTTGGTTATT	6	0.15	No Hit
TGACGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGG	6	0.15	No Hit
TGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATC	6	0.15	No Hit
TCTGAGGCGGAGGGCAATTCTCCATAAAAGGGACGGGGTTTTGCCATTCG	6	0.15	No Hit
CTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATT	6	0.15	No Hit
GGGCAGAAACATAGCACCCAAGTCTGAAACAATTGGCACCAGTCTTCCCT	6	0.15	No Hit
CGGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	6	0.15	No Hit
TCCACACACATACACACACACAC	6	0.15	No Hit
CATTGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGA	6	0.15	No Hit
TACGCTACTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACC	5	0.125	No Hit
AGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
TGAAAGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCC	5	0.125	No Hit
GCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTAC	5	0.125	No Hit
GCCCCACACCTAGCCCTCCAGAAGACAATTTATGAATATACCTTCTTGTT	5	0.125	No Hit
GAGCAGCGAGAGATGGCATTAGAAGACCTGAAAAAGCTGCATGATGAAAA	5	0.125	No Hit
ACTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACA	5	0.125	No Hit
GGTGCTGTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
GGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGAT	5	0.125	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	5	0.125	No Hit
TCTGCGCATGTGCCCGGGGTAGTTTTCCACTCCATGTGCTCTGCCTTCCC	5	0.125	No Hit
CCTTCGGCGGAGGGCAATTCTCCATAAAAGGGACGGGGTTTTGCCATTCG	5	0.125	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	5	0.125	No Hit
GGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
GCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGC	5	0.125	No Hit
TGCGTGTTTTCTGGGAAAGGTGAAAAAAATGTTCTGTATCAAAGAGAAAC	5	0.125	No Hit
GCCTCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGCAGTCAAAACACATACACATAGACT	5	0.125	No Hit
GTCCTAAAACAAAAGAAAAGAAAACAAACAAACCCTAATCAAATCACAAA	5	0.125	No Hit
AAAAAACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTT	5	0.125	No Hit
TTGTCCTCTGAAAATGAATATAATTAAAGGATTCTCACTCC	5	0.125	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	5	0.125	No Hit
GTCTAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCC	5	0.125	No Hit
TGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACAATGTTACATCTT	5	0.125	No Hit
GGATGCCCACTGAGGATCATACCTAGGTAGGGAATCCTGAAATTTATTAG	5	0.125	No Hit
AGAAAATCAGCAAAGAATTGACCAGATGCTAGAATCTCATGCCTCAACTA	5	0.125	No Hit
GCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACC	5	0.125	No Hit
TTAATCAGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGA	5	0.125	No Hit
CCTCTGAAAATGAATATAATTAAAGGATTCTCACTCCAAACATGTTCTTA	5	0.125	No Hit
ACTGAAAAAGCTGCATGATGAAAAAATCGAGTCATTGAGAGCTGAATTTC	5	0.125	No Hit
AGGCAGGATGGTGTACAGACCTTCATATAATCTCAAGAGCAGTACTGCCT	5	0.125	No Hit
AGTCAGACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACC	5	0.125	No Hit
GACTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAA	5	0.125	No Hit
TAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAA	5	0.125	No Hit
ACTGCACTAGATGAATTTAAGGTGGAGAGAGAACTTGTTGAGAAAGAGTT	5	0.125	No Hit
GGTGTTGGTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGAT	5	0.125	No Hit
TGGATAAGAAGTATATTATCTACATTTGCTGTTTAATTGTTTTGATAAGGAATAAAGGGGATTCCCAC	5	0.125	No Hit
TGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTC	5	0.125	No Hit
AGAGAAATATTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGT	5	0.125	No Hit
TTTCTGCTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTA	5	0.125	No Hit
TTACTGTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGAAA	5	0.125	No Hit
GTGGGAATATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTT	5	0.125	No Hit
TTCCTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTA	5	0.125	No Hit
GGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
TGGCACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAG	5	0.125	No Hit
ACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAG	5	0.125	No Hit
CGGCGGCATGAGAAGTGTTTGGAGTTTTTTCTTTAGTTAAGTGCAGTAGG	5	0.125	No Hit
ACGAGGATATGAAATAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGC	5	0.125	No Hit
GCAGGGTCTCAGTGTCTTACCTGTGGGAGCATTGCAAAAAGTTTTTTGGG	5	0.125	No Hit
TCCCAGCACTTAGGAAGCAGAGGCAGGTGAATTTCTGAGCTCCAGGACAG	5	0.125	No Hit
GACGGGCTGCACATGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCA	5	0.125	No Hit
ACGTCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	5	0.125	No Hit
GACTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATGGATTCT	5	0.125	No Hit
TTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACAT	5	0.125	No Hit
TTGACCTTCAGAAGAAGATATTCATTGTATATTAACATTTAGAGGTCATT	5	0.125	No Hit
AAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGA	5	0.125	No Hit
GTAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTT	5	0.125	No Hit
GCAGAGGTTTGAAACTTTTTAAAGAAACTGTAGGACTCATCATTGTCAGC	5	0.125	No Hit
TTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTC	5	0.125	No Hit
TGCGGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAG	5	0.125	No Hit
TAGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATG	5	0.125	No Hit
AGGCTGCTCAGACCTGGAGATCAAGAACTGGGTTTGTTTGGTGGAAAACC	5	0.125	No Hit
CATACTGCCTTCTACTAAATCAGACTTGTGAGCCAGACGGGCTGCACATG	5	0.125	No Hit
TGGAGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACT	5	0.125	No Hit
TTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGA	5	0.125	No Hit
GATAGTGCAGGACTCACTCAAACCTCTCAGCATCTTAGGACTGTTTCAAG	5	0.125	No Hit
CACGACAATTATGTAGAAAAAAAACTGAAAGAACAAATAGTTGAATTGCA	5	0.125	No Hit
TCAAAAAATTCATGATAATAATGAGAGTTACCAGGTGGGTTTGTCAGAGT	5	0.125	No Hit
GAAATTGCACTAAAGGAAGCAGAAACAGATGAGATAAAGATCTTGTTGGA	5	0.125	No Hit
TGTTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAGCCAGAC	5	0.125	No Hit
TATAGCCGAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCT	5	0.125	No Hit
TCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATAC	5	0.125	No Hit
TTGCTGGCCTCCACGGGGACTGTATGAACATGGTACACAGACATTCATGC	5	0.125	No Hit
TACGTTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTTATTCTATC	5	0.125	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	5	0.125	No Hit
AGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGG	5	0.125	No Hit
ACACAGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGACAGACCACAATATGTCTTT	5	0.125	No Hit
TTGCTTGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAA	5	0.125	No Hit
CTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACAAATAGT	5	0.125	No Hit
TTGCGATACTGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTA	5	0.125	No Hit
AACTCGCCTGTATTAGAGAAATATTACTGTTACTTATATTCTTTATGATT	5	0.125	No Hit
TGGGAAATGTCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTATC	5	0.125	No Hit
CTTGATGCTCTAGTAAAAGACAGTGAAGAAAATGTAAATAAAATTTTAAA	5	0.125	No Hit
CAGGCCTCTCAGCATCTTAGGACTGTTTCAAGTAATCATATTGATGAACT	5	0.125	No Hit
TGGTGTGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTA	5	0.125	No Hit
TGTCCTAGATAGAATGAAGCCAAATGTCTGGATTTAAAACAATTTCAAAC	5	0.125	No Hit
TGTCCTCTGAAAATGAATATAATTAAAGGATTCTCACTCCAAACATGTTC	5	0.125	No Hit
GGAGCCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTT	5	0.125	No Hit
TGGATTCTTGTGGGTATTAAAGCTTTGGGTCTGTTACCAGC	5	0.125	No Hit
TTAATAGGGAGGCAGAGGCAGGTGGATCTCTGAGTTCGAGGCCAGCCTGG	5	0.125	No Hit
CACTGTAGACATGAAGAAGAATCTAATATACTTAAGGCTGAATTAGACAA	5	0.125	No Hit
GTGCTGGAATGTGGGAAAATGTCATGTTTTTCACAGTTTCTATCAATGTG	5	0.125	No Hit
ATTGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCA	5	0.125	No Hit
GAATGAGGAAATGCAAAATGTCAGAACCTCTTTGATTGCTGAGCAGCAGA	5	0.125	No Hit
GTTACTTATATTCTTTATGATTTTTGTATTTATTGTCCTCTGAAAATGAATATAATTAAAGGATTCTCACTCC	5	0.125	No Hit
AAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATGTCATGTT	5	0.125	No Hit
TGATAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.1	0.0	0.0	0.0	0.0
82-83	0.1	0.0	0.0	0.0	0.0
84-85	0.1125	0.0	0.0	0.0	0.0
86-87	0.125	0.0	0.0	0.0	0.0
88-89	0.125	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTACC	30	0.002487678	65.9898	82-83
TCTGTTA	35	0.005396333	54.344536	80-81
GGTCTGT	40	0.009905501	46.637024	78-79
TGGGTCT	40	0.009905501	46.637024	76-77
>>END_MODULE
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823359 spots for SRR25194572.sra
Written 823359 spots for SRR25194572.sra
Read 823372 spots for SRR25194572.sra
Written 823372 spots for SRR25194572.sra
SRR ids: ['SRR25194572.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x1jje_fp
SRR25194572.sra spots: 13173757
blocks: [[1, 823359], [823360, 1646718], [1646719, 2470077], [2470078, 3293436], [3293437, 4116795], [4116796, 4940154], [4940155, 5763513], [5763514, 6586872], [6586873, 7410231], [7410232, 8233590], [8233591, 9056949], [9056950, 9880308], [9880309, 10703667], [10703668, 11527026], [11527027, 12350385], [12350386, 13173757]]
SRR25194572 file size 390443
SRR25194572 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25194572 SRR25194572_1.fastq
Input file:	SRR25194572_1.fastq
trimmed:	SRR25194572-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Sun Jun 21 11:47:09 2026 >> started

Sun Jun 21 11:47:20 2026 >> done (10.722s)
13173757 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
13173757 (100.00%) reads available; of these:
      83 ( 0.00%) trimmed reads available after processing
13173674 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	    8280	  0.06%
 21	    8097	  0.06%
 22	    8402	  0.06%
 23	    8343	  0.06%
 24	    8817	  0.07%
 25	    9628	  0.07%
 26	   10462	  0.08%
 27	   11936	  0.09%
 28	   13359	  0.10%
 29	   15494	  0.12%
 30	   17920	  0.14%
 31	   21279	  0.16%
 32	   23694	  0.18%
 33	   24546	  0.19%
 34	   25223	  0.19%
 35	   25378	  0.19%
 36	   27525	  0.21%
 37	   28929	  0.22%
 38	   30919	  0.23%
 39	   33021	  0.25%
 40	   35346	  0.27%
 41	   37500	  0.28%
 42	   41397	  0.31%
 43	   44837	  0.34%
 44	   46955	  0.36%
 45	   45052	  0.34%
 46	   45800	  0.35%
 47	   46444	  0.35%
 48	   48589	  0.37%
 49	   50771	  0.39%
 50	   53489	  0.41%
 51	   55495	  0.42%
 52	   60636	  0.46%
 53	   63718	  0.48%
 54	   66294	  0.50%
 55	   71120	  0.54%
 56	   84421	  0.64%
 57	   86611	  0.66%
 58	   85047	  0.65%
 59	   78740	  0.60%
 60	   79253	  0.60%
 61	   78541	  0.60%
 62	   79593	  0.60%
 63	   80859	  0.61%
 64	   83077	  0.63%
 65	   82004	  0.62%
 66	   86400	  0.66%
 67	   88551	  0.67%
 68	   90275	  0.69%
 69	   94809	  0.72%
 70	   98403	  0.75%
 71	  101864	  0.77%
 72	  103021	  0.78%
 73	   96710	  0.73%
 74	   96623	  0.73%
 75	   96463	  0.73%
 76	   97924	  0.74%
 77	   98705	  0.75%
 78	  103307	  0.78%
 79	  113040	  0.86%
 80	  142759	  1.08%
 81	  170642	  1.30%
 82	     921	  0.01%
 83	   20705	  0.16%
 84	   78663	  0.60%
 85	   95517	  0.73%
 86	   97426	  0.74%
 87	  101052	  0.77%
 88	  101702	  0.77%
 89	  102745	  0.78%
 90	   99617	  0.76%
 91	  110881	  0.84%
 92	   68373	  0.52%
 93	   71830	  0.55%
 94	   97025	  0.74%
 95	  122130	  0.93%
 96	  149793	  1.14%
 97	  243075	  1.85%
 98	  396534	  3.01%
 99	  253095	  1.92%
100	 1081317	  8.21%
101	 6209019	 47.13%
13173757 reads passed initial QC


criterion=sequence-density
sequence-density=0.55
sequence-density-rank=1
fanout-score=3.86
fanout-score-rank=35
prefix-density=1.64
prefix-fanout=1.3
sequence=CTGTGCTGTGCAGCTAATCAGATTCTTGTTTTTTCCCACTTGGATTATGTTGATGTTAATACGCAGTGATTTCACATAGGATGATTTGTACTTGCTTACATTTTTACAATAAAATGATCTACATGGAAGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=60.94
fanout-score-rank=1
prefix-density=0.08
prefix-fanout=4.0
sequence=AGAAGCAGAGTCAGCAACCTCCCCACAGTGAAGAAGTTTCTTCAACCTGGCAGCCAGAGGAAGCCTTTTGATGACGCAAAATGTGTTGAGTCAGCAAAGAAGATTTTCAGTTAATTCAGGCAACTATAAGTACATAGCCCCCACAAAGCCAACCTTCTAAAATTTTGCATCACATTGAAGTGTTTTGACTAAGTGTTGACCCTACTTAGAAAGCTAACAAGTTTTCTAAGGCGTCTGTGTCAATTCAGGTAGACATGACTGACGAGGAACGGCCGGATGCTCTCTAGTTGTAGTTAAAATTGAAATCATGATCACTTCCTCGGATATTTTCTTACTGTTCAATAAAACAAAACAAGCTTCTTAGACTCTGGAGT
                                 Started job on |	Jun 21 11:47:37
                             Started mapping on |	Jun 21 11:47:38
                                    Finished on |	Jun 21 11:48:07
       Mapping speed, Million of reads per hour |	1635.36

                          Number of input reads |	13173757
                      Average input read length |	88
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11324448
                        Uniquely mapped reads % |	85.96%
                          Average mapped length |	87.43
                       Number of splices: Total |	1251879
            Number of splices: Annotated (sjdb) |	1240756
                       Number of splices: GT/AG |	1243961
                       Number of splices: GC/AG |	4199
                       Number of splices: AT/AC |	447
               Number of splices: Non-canonical |	3272
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.44
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.15
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1737569
             % of reads mapped to multiple loci |	13.19%
        Number of reads mapped to too many loci |	60912
             % of reads mapped to too many loci |	0.46%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.38%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	111740	111740	111740
N_multimapping	1737569	1737569	1737569
N_noFeature	839220	872536	10372403
N_ambiguous	1053417	135355	609238
UnstrandedReadsAssigned:9431811 PositiveStrandReadsAssigned:10316557 NegativeStrandReadsAssigned:342807
Dataset is classified positive stranded
MeadianReadLen=101 20thPercentileLength=80 echo kmer=75
SRR25194572 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25194572-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,173,757 reads, 8,503,557 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,069 rounds

 109283 SRR25194572.ke.tsv
  52636 SRR25194572.se.tsv
 161919 total
==> SRR25194572.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25194572.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25194572 completed mapping pipeline successfully
