Starting /dee2/code/volunteer_pipeline.sh SRR25222511
    current disk space = 429192302592
    free memory = 394940196 
SRR25222511 SRAfilesize
a78f873d982295c23b80f0f60e0efcc2  SRR25222511.sra
SRR25222511.sra file validated
SRR25222511 is single end
SRR25222511 is conventional basespace
SRR25222511 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25222511_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.02	37.0	37.0	37.0	25.0	37.0
2	35.931	37.0	37.0	37.0	37.0	37.0
3	35.766	37.0	37.0	37.0	37.0	37.0
4	36.0475	37.0	37.0	37.0	37.0	37.0
5	35.8795	37.0	37.0	37.0	37.0	37.0
6	35.032	37.0	37.0	37.0	25.0	37.0
7	35.4185	37.0	37.0	37.0	37.0	37.0
8	35.971	37.0	37.0	37.0	37.0	37.0
9	35.9525	37.0	37.0	37.0	37.0	37.0
10-14	35.535199999999996	37.0	37.0	37.0	34.6	37.0
15-19	35.4207	37.0	37.0	37.0	34.6	37.0
20-24	35.0997	37.0	37.0	37.0	29.8	37.0
25-29	35.3819	37.0	37.0	37.0	32.2	37.0
30-34	35.161899999999996	37.0	37.0	37.0	34.6	37.0
35-39	35.2868	37.0	37.0	37.0	32.2	37.0
40-44	34.87480000000001	37.0	37.0	37.0	29.8	37.0
45-49	34.923700000000004	37.0	37.0	37.0	29.8	37.0
50-54	34.9927	37.0	37.0	37.0	32.2	37.0
55-59	34.659000000000006	37.0	37.0	37.0	32.2	37.0
60-64	35.289100000000005	37.0	37.0	37.0	32.2	37.0
65-69	34.66250000000001	37.0	37.0	37.0	27.4	37.0
70-74	34.0885	37.0	37.0	37.0	22.2	37.0
75-79	34.3981	37.0	37.0	37.0	25.0	37.0
80-84	34.7552	37.0	37.0	37.0	25.0	37.0
85-89	34.268899999999995	37.0	37.0	37.0	25.0	37.0
90-94	33.9135	37.0	37.0	37.0	22.2	37.0
95-99	34.1409	37.0	37.0	37.0	25.0	37.0
100-104	32.5834	37.0	32.2	37.0	19.4	37.0
105-109	33.6333	37.0	37.0	37.0	22.2	37.0
110-114	33.2999	37.0	37.0	37.0	16.6	37.0
115-119	33.331900000000005	37.0	37.0	37.0	16.6	37.0
120-124	32.7701	37.0	34.6	37.0	19.4	37.0
125-129	33.4172	37.0	37.0	37.0	19.4	37.0
130-134	32.6437	37.0	34.6	37.0	13.8	37.0
135-139	32.3757	37.0	32.2	37.0	11.0	37.0
140-144	31.763799999999996	37.0	27.4	37.0	11.0	37.0
145-149	32.2539	37.0	34.6	37.0	11.0	37.0
150	32.846	37.0	37.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	1.0
16	0.0
17	3.0
18	2.0
19	1.0
20	5.0
21	5.0
22	6.0
23	5.0
24	15.0
25	23.0
26	29.0
27	46.0
28	60.0
29	91.0
30	132.0
31	205.0
32	280.0
33	399.0
34	707.0
35	930.0
36	1018.0
37	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1	16.45	10.525	30.925000000000004
2	16.150000000000002	20.025000000000002	35.525	28.299999999999997
3	16.650000000000002	23.200000000000003	29.425	30.725
4	10.925	16.900000000000002	34.849999999999994	37.325
5	11.975	37.15	33.25	17.625
6	27.175	36.875	17.8	18.15
7	24.224999999999998	31.8	22.425	21.55
8	23.3	35.375	20.775	20.549999999999997
9	23.825	19.15	16.675	40.35
10-14	21.02	23.62	29.025000000000002	26.334999999999997
15-19	20.01	29.755	25.27	24.965
20-24	21.224999999999998	27.93	26.855	23.990000000000002
25-29	21.68	28.110000000000003	25.869999999999997	24.34
30-34	20.745	28.945	26.174999999999997	24.135
35-39	20.785	28.18	26.015	25.019999999999996
40-44	19.845	29.549999999999997	25.86	24.745
45-49	20.19	30.11	24.605	25.095
50-54	19.715	29.925	26.445	23.915
55-59	19.465	30.89	25.779999999999998	23.865
60-64	19.615	31.019999999999996	25.505	23.86
65-69	18.465	33.21	24.815	23.51
70-74	18.595	32.34	25.85	23.215
75-79	18.279999999999998	33.03	25.56	23.13
80-84	19.045	33.645	23.96	23.35
85-89	19.165	34.795	24.36	21.68
90-94	18.490000000000002	35.75	23.97	21.790000000000003
95-99	19.475	35.045	23.630000000000003	21.85
100-104	18.305	35.9	24.505	21.29
105-109	17.83	37.385000000000005	24.255	20.53
110-114	18.16	37.12	23.695	21.025
115-119	18.7	36.605	24.465	20.23
120-124	17.28	38.795	23.135	20.79
125-129	16.335	39.515	23.565	20.585
130-134	17.59	39.519999999999996	22.49	20.4
135-139	17.59	39.15	22.905	20.355
140-144	18.09	39.915	21.755	20.24
145-149	17.495	39.434999999999995	23.1	19.97
150	18.9	39.125	22.3	19.675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	1.5
3	2.5
4	4.0
5	5.5
6	4.0
7	4.0
8	5.5
9	5.5
10	4.5
11	7.0
12	7.5
13	8.0
14	12.0
15	11.0
16	11.5
17	11.5
18	10.0
19	12.0
20	16.5
21	18.0
22	14.0
23	14.0
24	18.5
25	23.0
26	28.5
27	28.5
28	24.5
29	25.5
30	34.0
31	45.5
32	54.0
33	63.0
34	83.5
35	97.0
36	110.5
37	130.5
38	109.0
39	105.0
40	126.0
41	106.5
42	92.0
43	97.5
44	124.5
45	151.0
46	208.0
47	242.5
48	200.0
49	176.0
50	182.0
51	148.5
52	103.5
53	98.5
54	100.5
55	91.5
56	75.0
57	73.0
58	76.5
59	75.5
60	71.0
61	59.5
62	41.0
63	21.5
64	13.0
65	15.0
66	10.5
67	10.5
68	12.0
69	6.5
70	3.0
71	1.5
72	0.5
73	0.5
74	0.5
75	1.0
76	0.5
77	0.5
78	2.0
79	1.5
80	1.0
81	2.0
82	1.5
83	1.0
84	0.5
85	0.0
86	0.0
87	0.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.14331323164242	59.3
2	9.790705924086556	13.8
3	2.1284143313231643	4.5
4	1.0287335934728628	2.9000000000000004
5	0.8513657325292656	3.0
6	0.5321035828307911	2.25
7	0.35473572188719404	1.7500000000000002
8	0.17736786094359702	1.0
9	0.0709471443774388	0.44999999999999996
>10	0.9223128769067045	11.05
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCTACCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAA	41	1.0250000000000001	No Hit
CTCCATAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGT	36	0.8999999999999999	No Hit
GTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTAC	26	0.65	No Hit
CTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAAT	25	0.625	No Hit
GGTCCGCTGTGTTCAACGTTTCTAGAATGCTGTTCTTGGCTAATGGCCAA	23	0.575	No Hit
GTGCTACTATCATCCACACATTTCTACCTGAGACCACCCAAAGGACCCTC	23	0.575	No Hit
ACCAATTCCTCCACATCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTA	22	0.5499999999999999	No Hit
GACCAGTACTCTACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCT	21	0.525	No Hit
TCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGCATTCCTC	20	0.5	No Hit
AGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTACTGCTA	15	0.375	No Hit
CTCCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCT	15	0.375	No Hit
GGTACAACTTGCCCTCTGAAGAGGAAGAACAGAAGGATGCCACAACTCTC	14	0.35000000000000003	No Hit
ACAATAAACCATTCAAAGATGTAAAGCTTTTTAAATTGCCAAGGTCCGCT	14	0.35000000000000003	No Hit
GAGGGAGGATTCAGAATTTTCAAATTATTCACAGCGCTGCCAAGTTCACC	13	0.325	No Hit
ATTTTCAAATTATTCACAGCGCTGCCAAGTTCACCAAGAAGTGACTCTGA	13	0.325	No Hit
GTGTTCAACGTTTCTAGAATGCTGTTCTTGGCTAATGGCCAACTTGTGGC	12	0.3	No Hit
CAACAATAAACCATTCAAAGATGTAAAGCTTTTTAAATTGCCAAGGTCCG	12	0.3	No Hit
TAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGG	11	0.27499999999999997	No Hit
GTCTTTACCTAAGCATTATTCAAAATGAAAATAAAGAGCTGAGCAGCATT	11	0.27499999999999997	No Hit
GCTTCAGAGGGAGGATTCAGAATTTTCAAATTATTCACAGCGCTGCCAAG	11	0.27499999999999997	No Hit
GGTCAGAAGCGGTCTGGTCAGTTATTTTTGAGGGGTGGAAGACCACACCC	11	0.27499999999999997	No Hit
CTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTA	11	0.27499999999999997	No Hit
GACCAGTACTATACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCT	11	0.27499999999999997	No Hit
GTTCACCAAGAAGTGACTCTGAGTTCCCAATGAGCAGGCCACACAAGACA	11	0.27499999999999997	No Hit
GAACCAAGGATGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCAT	10	0.25	No Hit
CATTTCTACCTGAGACCACCCAAAGGACCCTCCCAAATTCTCTTCCTCTC	10	0.25	No Hit
TCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCTACTATCATCCACAC	9	0.22499999999999998	No Hit
CCTCTAGAAAGTGCTACTATCATCCACACATTTCTACCTGAGACCACCCA	9	0.22499999999999998	No Hit
GAACAGAAGGATGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCA	8	0.2	No Hit
CACTGAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCTACTGGCAGGA	8	0.2	No Hit
GCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAATTC	8	0.2	No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT	8	0.2	No Hit
GCAACTCCACATCCAGATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGA	8	0.2	No Hit
GTTCCCAATGAGCAGGCCACACAAGACAACAAGGCTCCCAAGTTGGAACA	7	0.17500000000000002	No Hit
CTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTACTGCTAGAGAT	7	0.17500000000000002	No Hit
GAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCTACTGGCAGGACAGG	7	0.17500000000000002	No Hit
GTTGTTAACAATGGCTATGATTACAATCATTTAGATCTATGATTTGGGCT	7	0.17500000000000002	No Hit
CCTCCACCTACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACA	7	0.17500000000000002	No Hit
CTCCACCTCCTTGTCCACCAGTTCCTCCACATCCTAGACCTCCAAGCAAT	7	0.17500000000000002	No Hit
TTCCTGAGGTGACCATAGGCAAATGCTCCAGTAATACAGATAAAATCTCC	7	0.17500000000000002	No Hit
GGGGCAGGGTGAGAGGGAAAAGACTTCGACGGACGTGCAAAGAAATCAAG	7	0.17500000000000002	No Hit
CCTTCTAGCTGATACTGCTATCGTCAGGGGAAGATGACCAGGAACGAGAA	7	0.17500000000000002	No Hit
CTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAA	7	0.17500000000000002	No Hit
CCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCATCTTACTTCTGAT	6	0.15	No Hit
GTACTATACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGC	6	0.15	No Hit
ACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTAC	6	0.15	No Hit
GAGTTCCCAATGAGCAGGCCACACAAGACAACAAGGCTCCCAAGTTGGAA	6	0.15	No Hit
ATTCTGGATGGTGTCTCTACCCTCCTTACAAGTACATTGTCAACCGTCCT	6	0.15	No Hit
GTTCTTGGCTAATGGCCAACTTGTGGCTTGCTGAAGCAACTCCACATCCA	6	0.15	No Hit
AGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAATTCCCTTT	6	0.15	No Hit
GCTTAGCTTGAAAATCCAGGACTTTGAGATTATTGATTTTCAACAATAAA	6	0.15	No Hit
CAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCT	6	0.15	No Hit
TCTCTGAGTAGTCTCCACACCTGTTACCACCATCCCAGAATTAAAATCCT	6	0.15	No Hit
CCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGCATTCCTCCTACCGG	6	0.15	No Hit
CTACTCTCCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAA	6	0.15	No Hit
CCCTTTTGCAGCAAGAATAAAATCAACCAAAGCACTGTGGAATTTTGGGA	6	0.15	No Hit
GTATTACTGGAGCATTTGCCTATGGTCACCTCAGGAAGGCCAGTCTGGGC	6	0.15	No Hit
GTCTAAGGAAGAGCACATCATTGCAGCCCAGGGCACATACACTCCTTCGG	6	0.15	No Hit
CCTCCTACCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCAT	5	0.125	No Hit
CCTTTACTCCTCACCAAAAGTAACTATCTTCCCTTTAACCTTATGCAATA	5	0.125	No Hit
GGTTTGCTTTGAAGAAGGATGCACACGAGAGAGATCCTTGTTTCTCTGCA	5	0.125	No Hit
ACCACACCCTCCACCCTTTGGTCCAGGAATTGGTAGACCACCCCCTCCAC	5	0.125	No Hit
GATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGATTCAGAATTTTCAAA	5	0.125	No Hit
CTCCACTCTGCTCGCTGCCATCGTGGCAGTAACTGCTTGTCTCCCCGTGC	5	0.125	No Hit
GCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTACAATGCCA	5	0.125	No Hit
GAATTTTCAAATTATTCACAGCGCTGCCAAGTTCACCAAGAAGTGACTCT	5	0.125	No Hit
GCATTATAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.125	No Hit
ACCTGAGACCACCCAAAGGACCCTCCCAAATTCTCTTCCTCTCTGAGTAG	5	0.125	No Hit
GAACCAAGGAGGCTTCCCCAGCCAAGGGCACCGTCAGATCAATGCCATTG	5	0.125	No Hit
TGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCATCTTACTTCTG	5	0.125	No Hit
GGCACATACTGTGATGTCATTTCTGGAGATAAAGTCGATGGCAATTGCAC	5	0.125	No Hit
GCTCAAGGGAGCTTGTGTCTCTTGGAGGGCAGGTGGCATAAAGAGTATCT	5	0.125	No Hit
CACCTACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCA	5	0.125	No Hit
CTTCAGTACGTCCTCAGCACACTGAATCCAAGTGTTCTTCAGGGTCTCCT	5	0.125	No Hit
CCCAAAGGACCCTCCCAAATTCTCTTCCTCTCTGAGTAGTCTCCACACCT	5	0.125	No Hit
CACAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTTTGAAG	5	0.125	No Hit
CCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACT	5	0.125	No Hit
GCTAGGATTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA	5	0.125	No Hit
GGTATTATGTGGGCAATTCTGAGTACTCTAGCTTCCCTAACGATCCCAGC	5	0.125	No Hit
CTCTCCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAAGTG	5	0.125	No Hit
GATAATGACAGCAACCAGGTAGCTTTTGGCAGAGGAAACAAAGGATTCAT	5	0.125	No Hit
CCTCCTACCGGACCTCCTACCACAGTACAAGCAACTACAATGCCAGCTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.05	0.0	0.0	0.0	0.0
16-17	0.07500000000000001	0.0	0.0	0.0	0.0
18-19	0.1	0.0	0.0	0.0	0.0
20-21	0.125	0.0	0.0	0.0	0.0
22-23	0.21250000000000002	0.0	0.0	0.0	0.0
24-25	0.25	0.0	0.0	0.0	0.0
26-27	0.2875	0.0	0.0	0.0	0.0
28-29	0.3	0.0	0.0	0.0	0.0
30-31	0.3	0.0	0.0	0.0	0.0
32-33	0.3	0.0	0.0	0.0	0.0
34-35	0.3	0.0	0.0	0.0	0.0
36-37	0.3	0.0	0.0	0.0	0.0
38-39	0.3	0.0	0.0	0.0	0.0
40-41	0.3	0.0	0.0	0.0	0.0
42-43	0.3	0.0	0.0	0.0	0.0
44-45	0.3	0.0	0.0	0.0	0.0
46-47	0.3	0.0	0.0	0.0	0.0
48-49	0.3	0.0	0.0	0.0	0.0
50-51	0.3	0.0	0.0	0.0	0.0
52-53	0.3	0.0	0.0	0.0	0.0
54-55	0.3	0.0	0.0	0.0	0.0
56-57	0.3	0.0	0.0	0.0	0.0
58-59	0.3	0.0	0.0	0.0	0.0
60-61	0.3	0.0	0.0	0.0	0.0
62-63	0.3	0.0	0.0	0.0	0.0
64-65	0.3	0.0	0.0	0.0	0.0
66-67	0.3	0.0	0.0	0.0	0.0
68-69	0.3	0.0	0.0	0.0	0.0
70-71	0.3	0.0	0.0	0.0	0.0
72-73	0.3125	0.0	0.0	0.0	0.0
74-75	0.325	0.0	0.0	0.0	0.0
76-77	0.325	0.0	0.0	0.0	0.0
78-79	0.325	0.0	0.0	0.0	0.0
80-81	0.325	0.0	0.0	0.0	0.0
82-83	0.35	0.0	0.0	0.0	0.0
84-85	0.35	0.0	0.0	0.0	0.0
86-87	0.35	0.0	0.0	0.0	0.0
88-89	0.35	0.0	0.0	0.0	0.0
90-91	0.35	0.0	0.0	0.0	0.0
92-93	0.35	0.0	0.0	0.0	0.0
94-95	0.375	0.0	0.0	0.0	0.0
96-97	0.375	0.0	0.0	0.0	0.0
98-99	0.375	0.0	0.0	0.0	0.0
100-101	0.375	0.0	0.0	0.0	0.0
102-103	0.375	0.0	0.0	0.0	0.0
104-105	0.375	0.0	0.0	0.0	0.0
106-107	0.375	0.0	0.0	0.0	0.0
108-109	0.375	0.0	0.0	0.0	0.0
110-111	0.375	0.0	0.0	0.0	0.0
112-113	0.375	0.0	0.0	0.0	0.0
114-115	0.375	0.0	0.0	0.0	0.0
116-117	0.375	0.0	0.0	0.0	0.0
118-119	0.375	0.0	0.0	0.0	0.0
120-121	0.375	0.0	0.0	0.0	0.0
122-123	0.375	0.0	0.0	0.0	0.0
124-125	0.375	0.0	0.0	0.0	0.0
126-127	0.3875	0.0	0.0	0.0	0.0
128-129	0.4	0.0	0.0	0.0	0.0
130-131	0.4	0.0	0.0	0.0	0.0
132-133	0.425	0.0	0.0	0.0	0.0
134-135	0.425	0.0	0.0	0.0	0.0
136-137	0.4375	0.0	0.0	0.0	0.0
138	0.45	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGTAC	15	1.1730364E-4	144.0	3
CAGTACT	10	0.006973645	144.0	4
GGGAGCT	10	0.006973645	144.0	7
ACCAGTA	10	0.006973645	144.0	2
CTCTGAG	35	0.0036813593	20.571428	45-49
GGGGGGG	35	0.0036813593	20.571428	110-114
>>END_MODULE
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852416 spots for SRR25222511.sra
Written 852416 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
Read 852401 spots for SRR25222511.sra
Written 852401 spots for SRR25222511.sra
SRR ids: ['SRR25222511.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bl6kfzdf
SRR25222511.sra spots: 13638431
blocks: [[1, 852401], [852402, 1704802], [1704803, 2557203], [2557204, 3409604], [3409605, 4262005], [4262006, 5114406], [5114407, 5966807], [5966808, 6819208], [6819209, 7671609], [7671610, 8524010], [8524011, 9376411], [9376412, 10228812], [10228813, 11081213], [11081214, 11933614], [11933615, 12786015], [12786016, 13638431]]
SRR25222511 file size 1447514
SRR25222511 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25222511 SRR25222511_1.fastq
Input file:	SRR25222511_1.fastq
trimmed:	SRR25222511-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Fri Jun 19 20:09:33 2026 >> started

Fri Jun 19 20:09:49 2026 >> done (15.517s)
13638431 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
13638431 (100.00%) reads available; of these:
      86 ( 0.00%) trimmed reads available after processing
13638345 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
143	       2	  0.00%
144	       0	  0.00%
145	       0	  0.00%
146	       8	  0.00%
147	      12	  0.00%
148	      36	  0.00%
149	      28	  0.00%
150	13638345	100.00%
13638431 reads passed initial QC


criterion=sequence-density
sequence-density=1.68
sequence-density-rank=1
fanout-score=1.99
fanout-score-rank=33
prefix-density=1.65
prefix-fanout=2.0
sequence=TACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGCGTCTTTCAAAACCATTATCATTTTCTCCAAGTACAATTGACTGGAATATCCATTGTAGTCTGTCAAATAAACTTCTTACAAGCATAAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=67.98
fanout-score-rank=1
prefix-density=0.30
prefix-fanout=3.9
sequence=AGAAGAAGAAGTGGTCCAAAGGCAAAGTTCGGGACAAGTTGAACAATCTTGTCCTGTTCGACAAAGCGACATACGACAAGCTCTGTAAGGAGGTTCCGAACTATAAGCTTATTACTCCAGCCGTGGTCTCTGAGAGACTGAAGATTCGCGGTTCCTTGGCCAGGGCAGCCCTTCAGGAGCTCCTTAGTAAAGGACTTATCAAGCTGGTTTCAAAGCACAGAGCCCAAGTAATTTACACCAGAAACACAAAGGGTGGGGACGCTCCAGCTGCTGGCGAAGATGCATGAACAGGTTCAATCAGCTGTACATTTGGAAAAATAAAACTTTATTGAATCA
                                 Started job on |	Jun 19 20:10:21
                             Started mapping on |	Jun 19 20:10:22
                                    Finished on |	Jun 19 20:11:21
       Mapping speed, Million of reads per hour |	832.18

                          Number of input reads |	13638431
                      Average input read length |	149
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11069601
                        Uniquely mapped reads % |	81.16%
                          Average mapped length |	145.62
                       Number of splices: Total |	5379089
            Number of splices: Annotated (sjdb) |	5303640
                       Number of splices: GT/AG |	5328604
                       Number of splices: GC/AG |	12584
                       Number of splices: AT/AC |	1675
               Number of splices: Non-canonical |	36226
                      Mismatch rate per base, % |	0.46%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.44
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	800806
             % of reads mapped to multiple loci |	5.87%
        Number of reads mapped to too many loci |	38838
             % of reads mapped to too many loci |	0.28%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.63%
                     % of reads unmapped: other |	0.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1768024	1768024	1768024
N_multimapping	800806	800806	800806
N_noFeature	264443	1592296	9468811
N_ambiguous	1360620	1058601	38150
UnstrandedReadsAssigned:9444538 PositiveStrandReadsAssigned:8418704 NegativeStrandReadsAssigned:1562640
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25222511 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25222511-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,638,431 reads, 9,215,814 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,270 rounds

 109283 SRR25222511.ke.tsv
  52636 SRR25222511.se.tsv
 161919 total
==> SRR25222511.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25222511.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25222511 completed mapping pipeline successfully
