Starting /dee2/code/volunteer_pipeline.sh SRR25222513
    current disk space = 1240550324
    free memory = 199223160 
SRR25222513 SRAfilesize
8c875bb2cdc51a9a2b5348d16dfe7506  SRR25222513.sra
SRR25222513.sra file validated
SRR25222513 is single end
SRR25222513 is conventional basespace
SRR25222513 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25222513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.326	37.0	37.0	37.0	37.0	37.0
2	35.922	37.0	37.0	37.0	37.0	37.0
3	35.8215	37.0	37.0	37.0	37.0	37.0
4	36.044	37.0	37.0	37.0	37.0	37.0
5	35.7845	37.0	37.0	37.0	37.0	37.0
6	34.8445	37.0	37.0	37.0	25.0	37.0
7	35.2145	37.0	37.0	37.0	37.0	37.0
8	35.9755	37.0	37.0	37.0	37.0	37.0
9	36.114	37.0	37.0	37.0	37.0	37.0
10-14	35.4425	37.0	37.0	37.0	34.6	37.0
15-19	35.304500000000004	37.0	37.0	37.0	34.6	37.0
20-24	35.031400000000005	37.0	37.0	37.0	29.4	37.0
25-29	35.415800000000004	37.0	37.0	37.0	32.2	37.0
30-34	35.157500000000006	37.0	37.0	37.0	34.6	37.0
35-39	35.3074	37.0	37.0	37.0	32.2	37.0
40-44	34.756800000000005	37.0	37.0	37.0	29.8	37.0
45-49	34.907500000000006	37.0	37.0	37.0	29.8	37.0
50-54	34.8751	37.0	37.0	37.0	29.8	37.0
55-59	34.7544	37.0	37.0	37.0	29.8	37.0
60-64	35.3107	37.0	37.0	37.0	32.2	37.0
65-69	34.3565	37.0	37.0	37.0	25.0	37.0
70-74	34.0737	37.0	34.6	37.0	22.2	37.0
75-79	34.058	37.0	37.0	37.0	22.2	37.0
80-84	34.5931	37.0	37.0	37.0	25.0	37.0
85-89	34.072199999999995	37.0	37.0	37.0	25.0	37.0
90-94	33.6467	37.0	37.0	37.0	22.2	37.0
95-99	34.019999999999996	37.0	37.0	37.0	25.0	37.0
100-104	32.2743	37.0	32.2	37.0	16.6	37.0
105-109	33.2741	37.0	34.6	37.0	19.4	37.0
110-114	33.084500000000006	37.0	34.6	37.0	19.4	37.0
115-119	33.0013	37.0	37.0	37.0	13.8	37.0
120-124	32.410700000000006	37.0	32.2	37.0	16.6	37.0
125-129	33.4449	37.0	37.0	37.0	22.2	37.0
130-134	32.724900000000005	37.0	34.6	37.0	13.8	37.0
135-139	32.3822	37.0	32.2	37.0	11.0	37.0
140-144	31.762300000000003	37.0	29.8	37.0	11.0	37.0
145-149	32.2021	37.0	32.2	37.0	11.0	37.0
150	33.134	37.0	37.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	1.0
21	5.0
22	5.0
23	16.0
24	10.0
25	29.0
26	31.0
27	49.0
28	79.0
29	87.0
30	150.0
31	210.0
32	271.0
33	475.0
34	694.0
35	956.0
36	911.0
37	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.975	16.475	11.25	30.3
2	15.525	20.1	33.375	31.0
3	14.149999999999999	23.525	32.0	30.325000000000003
4	10.525	17.775	33.925	37.775
5	10.95	38.5	32.75	17.8
6	26.474999999999998	36.525	17.875	19.125
7	22.85	34.300000000000004	21.099999999999998	21.75
8	21.349999999999998	38.125	19.775000000000002	20.75
9	21.825	20.05	18.125	40.0
10-14	20.825	23.810000000000002	29.365000000000002	26.0
15-19	19.645000000000003	29.94	24.66	25.755
20-24	20.685000000000002	27.48	27.235	24.6
25-29	21.39	28.050000000000004	25.47	25.09
30-34	20.925	28.715000000000003	25.485000000000003	24.875
35-39	20.03	28.065	27.165	24.740000000000002
40-44	20.380000000000003	29.565	26.0	24.055
45-49	20.31	28.935	25.235000000000003	25.52
50-54	19.515	29.565	27.025	23.895
55-59	19.900000000000002	30.735	25.445	23.919999999999998
60-64	19.56	29.4	26.715	24.325
65-69	18.92	32.190000000000005	25.27	23.62
70-74	18.82	30.11	26.3	24.77
75-79	18.509999999999998	31.730000000000004	26.314999999999998	23.445
80-84	20.115	32.035000000000004	24.654999999999998	23.195
85-89	19.345000000000002	32.005	25.395	23.255
90-94	19.400000000000002	33.755	24.635	22.21
95-99	19.305	32.875	24.665	23.155
100-104	17.805	33.945	25.31	22.939999999999998
105-109	18.4	34.11	25.575	21.915000000000003
110-114	18.685	33.96	25.335	22.02
115-119	19.405	33.894999999999996	24.845	21.855
120-124	17.83	37.885000000000005	22.425	21.86
125-129	17.26	38.080000000000005	22.75	21.91
130-134	17.544999999999998	37.68	22.830000000000002	21.945
135-139	18.459999999999997	37.445	22.905	21.19
140-144	18.61	38.824999999999996	21.88	20.685000000000002
145-149	18.325	37.62	23.28	20.775
150	17.925	37.175000000000004	24.9	20.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.5
5	3.0
6	5.5
7	4.5
8	2.5
9	2.5
10	1.5
11	3.5
12	5.5
13	5.0
14	8.5
15	9.5
16	9.0
17	10.5
18	8.5
19	5.5
20	8.0
21	11.5
22	9.0
23	12.5
24	14.0
25	19.5
26	19.0
27	13.5
28	19.5
29	27.0
30	32.0
31	38.0
32	45.0
33	61.5
34	84.5
35	102.0
36	121.5
37	118.5
38	95.0
39	115.0
40	128.5
41	94.0
42	94.0
43	112.5
44	136.0
45	168.0
46	228.5
47	277.5
48	237.0
49	202.5
50	200.0
51	160.5
52	140.0
53	118.5
54	87.0
55	80.0
56	77.5
57	77.5
58	67.0
59	63.5
60	58.5
61	37.5
62	19.0
63	17.0
64	15.5
65	9.5
66	6.5
67	8.5
68	5.0
69	2.0
70	3.0
71	1.5
72	1.0
73	1.0
74	0.5
75	1.5
76	1.5
77	0.5
78	0.5
79	0.5
80	1.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.26433004746258	57.699999999999996
2	8.689302665206279	11.899999999999999
3	2.9207740051113547	6.0
4	0.9492515516611902	2.6
5	0.6206644760861628	2.125
6	0.43811610076670315	1.7999999999999998
7	0.32858707557502737	1.575
8	0.32858707557502737	1.7999999999999998
9	0.2555677254472435	1.575
>10	1.1683096020445418	11.65
>50	0.03650967506389193	1.275
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCTACCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAA	51	1.275	No Hit
CTCCATAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGT	46	1.15	No Hit
GGTCCGCTGTGTTCAACGTTTCTAGAATGCTGTTCTTGGCTAATGGCCAA	25	0.625	No Hit
GTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTAC	25	0.625	No Hit
ATTTTCAAATTATTCACAGCGCTGCCAAGTTCACCAAGAAGTGACTCTGA	24	0.6	No Hit
GTGCTACTATCATCCACACATTTCTACCTGAGACCACCCAAAGGACCCTC	22	0.5499999999999999	No Hit
GAACCAAGGATGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCAT	18	0.44999999999999996	No Hit
ACCAATTCCTCCACATCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTA	17	0.42500000000000004	No Hit
ACAATAAACCATTCAAAGATGTAAAGCTTTTTAAATTGCCAAGGTCCGCT	17	0.42500000000000004	No Hit
GGTACAACTTGCCCTCTGAAGAGGAAGAACAGAAGGATGCCACAACTCTC	16	0.4	No Hit
GAACAGAAGGATGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCA	14	0.35000000000000003	No Hit
GACCAGTACTCTACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCT	14	0.35000000000000003	No Hit
CCTTACAAGTACATTGTCAACCGTCCTGCAAAACTTCCTATGTCCATTGC	13	0.325	No Hit
CTCTAATCTACTGGCAGGACAGGTACAACTTGCCCTCTGAAGAGGAAGAA	13	0.325	No Hit
CTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAAT	13	0.325	No Hit
CATTTCTACCTGAGACCACCCAAAGGACCCTCCCAAATTCTCTTCCTCTC	12	0.3	No Hit
GAATAAAATCAACCAAAGCACTGTGGAATTTTGGGAGAAATAACCTTTAA	11	0.27499999999999997	No Hit
GTCTTTACCTAAGCATTATTCAAAATGAAAATAAAGAGCTGAGCAGCATT	11	0.27499999999999997	No Hit
GAACCAAGGAGGCTTCCCCAGCCAAGGGCACCGTCAGATCAATGCCATTG	11	0.27499999999999997	No Hit
GGCACATACTGTGATGTCATTTCTGGAGATAAAGTCGATGGCAATTGCAC	11	0.27499999999999997	No Hit
CCCTCCACCCTTTGGTCCAGGAATTGGTAGACCACCCCCTCCACCCTTTG	11	0.27499999999999997	No Hit
GCAACTCCACATCCAGATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGA	11	0.27499999999999997	No Hit
CAACAATAAACCATTCAAAGATGTAAAGCTTTTTAAATTGCCAAGGTCCG	11	0.27499999999999997	No Hit
GATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGATTCAGAATTTTCAAA	10	0.25	No Hit
GCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTACAATGCCA	10	0.25	No Hit
CTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTACTGCTAGAGAT	10	0.25	No Hit
TAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGG	10	0.25	No Hit
GAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCTACTGGCAGGACAGG	10	0.25	No Hit
GCTTAGCTTGAAAATCCAGGACTTTGAGATTATTGATTTTCAACAATAAA	10	0.25	No Hit
CCATAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTG	10	0.25	No Hit
GATGTAAAGCTTTTTAAATTGCCAAGGTCCGCTGTGTTCAACGTTTCTAG	10	0.25	No Hit
GTTCACCAAGAAGTGACTCTGAGTTCCCAATGAGCAGGCCACACAAGACA	10	0.25	No Hit
CTCCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCT	10	0.25	No Hit
GAGGGAGGATTCAGAATTTTCAAATTATTCACAGCGCTGCCAAGTTCACC	9	0.22499999999999998	No Hit
CTGAAGAGGAAGAACAGAAGGATGCCACAACTCTCCTGCTGGCTACTCTC	9	0.22499999999999998	No Hit
GGTCAGAAGCGGTCTGGTCAGTTATTTTTGAGGGGTGGAAGACCACACCC	9	0.22499999999999998	No Hit
CACAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTTTGAAG	9	0.22499999999999998	No Hit
GCTAGGATTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA	9	0.22499999999999998	No Hit
GTCTAAGGAAGAGCACATCATTGCAGCCCAGGGCACATACACTCCTTCGG	9	0.22499999999999998	No Hit
CCTTCTAGCTGATACTGCTATCGTCAGGGGAAGATGACCAGGAACGAGAA	9	0.22499999999999998	No Hit
TCTCTGCACCTCATAAATTCACTCTCCATTAAAACCAATGCCCAGACTGG	8	0.2	No Hit
ACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTAC	8	0.2	No Hit
TGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCATCTTACTTCTG	8	0.2	No Hit
TCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGCATTCCTC	8	0.2	No Hit
GTTCTTGGCTAATGGCCAACTTGTGGCTTGCTGAAGCAACTCCACATCCA	8	0.2	No Hit
GGCTCCCAAGTTGGAACATCTTTGGTTTGCTTTGAAGAAGGATGCACACG	8	0.2	No Hit
GGCCACACAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTT	8	0.2	No Hit
GACCAGTACTATACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCT	8	0.2	No Hit
CCTCTAGAAAGTGCTACTATCATCCACACATTTCTACCTGAGACCACCCA	8	0.2	No Hit
GTGTTCAACGTTTCTAGAATGCTGTTCTTGGCTAATGGCCAACTTGTGGC	7	0.17500000000000002	No Hit
GCTTCAGAGGGAGGATTCAGAATTTTCAAATTATTCACAGCGCTGCCAAG	7	0.17500000000000002	No Hit
CATCCAGATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGATTCAGAATT	7	0.17500000000000002	No Hit
CCTCCACCTACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACA	7	0.17500000000000002	No Hit
GTATTCTGGATGGTGTCTCTACCCTCCTTACAAGTACATTGTCAACCGTC	7	0.17500000000000002	No Hit
GTCCAGGAATTGGTAGACCACACCCTCCACCCTTTGGTCCAGGAATTGGT	7	0.17500000000000002	No Hit
CCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGCATTCCTCCTACCGG	7	0.17500000000000002	No Hit
CCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACT	7	0.17500000000000002	No Hit
ACCCAAAGGACCCTCCCAAATTCTCTTCCTCTCTGAGTAGTCTCCACACC	7	0.17500000000000002	No Hit
GGTTTGCTTTGAAGAAGGATGCACACGAGAGAGATCCTTGTTTCTCTGCA	6	0.15	No Hit
GTCTTTAACAATGATGACTGGGCTTTGTCAGAAACTTTACAGACTGGTCT	6	0.15	No Hit
ATCATCAACAGTATTCTGGATGGTGTCTCTACCCTCCTTACAAGTACATT	6	0.15	No Hit
CTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTACA	6	0.15	No Hit
CCCAAATTCTCTTCCTCTCTGAGTAGTCTCCACACCTGTTACCACCATCC	6	0.15	No Hit
GGTTTTAATGGAGAGTGAATTTATGAGGTCCAAGGAAACAGAAATGTCGA	6	0.15	No Hit
CACCTACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCA	6	0.15	No Hit
CTCCTGCTGGCTACTCTCCAGTGGTTTCATCTTACTTCTGATGGCATTTC	6	0.15	No Hit
TCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCTACTATCATCCACAC	6	0.15	No Hit
CCAAAGCACTGTGGAATTTTGGGAGAAATAACCTTTAAAGGCTTAAGAAA	6	0.15	No Hit
GGTATTATGTGGGCAATTCTGAGTACTCTAGCTTCCCTAACGATCCCAGC	6	0.15	No Hit
CAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTTTGAAGAA	6	0.15	No Hit
CCTCCACCCTTTGGTCCAGGAATTGGTCGACCACCCCCTCCACCTCCTTG	5	0.125	No Hit
GCATTATTCAAAATGAAAATAAAGAGCTGAGCAGCATTATAAAGCAAAAA	5	0.125	No Hit
CCATTAAAACCAATGCCCAGACTGGCCTTCCTGAGGTGACCATAGGCAAA	5	0.125	No Hit
GCTTCAGTACGTCCTCAGCACACTGAATCCAAGTGTTCTTCAGGGTCTCC	5	0.125	No Hit
GAGTTCCCAATGAGCAGGCCACACAAGACAACAAGGCTCCCAAGTTGGAA	5	0.125	No Hit
ATTCTGGATGGTGTCTCTACCCTCCTTACAAGTACATTGTCAACCGTCCT	5	0.125	No Hit
GGTCTCCTCTCTAATCTACTGGCAGGACAGGTACAACTTGCCCTCTGAAG	5	0.125	No Hit
AGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAATTCCCTTT	5	0.125	No Hit
GGTACATACCCACCATTCTTAAATCAAGGCTACCCGTGGTATTATTATTA	5	0.125	No Hit
GCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAATTC	5	0.125	No Hit
GGACGGTTACAACTTGCCCTCTGAAGAGGAAGAACAGAAGGATGCCACAA	5	0.125	No Hit
TCCCTCTAGAAAGTGCTACTATCATCCACACATTTCTACCTGAGACCACC	5	0.125	No Hit
CTCCTTACAAGTACATTGTCAACCGTCCTGCAAAACTTCCTATGTCCATT	5	0.125	No Hit
GTCTCTACCCTCCTTACAAGTACATTGTCAACCGTCCTGCAAAACTTCCT	5	0.125	No Hit
CATACACTCCTTCGGCTTTAACTCAGATAATCTTTACGACCAACCAGCCT	5	0.125	No Hit
CCTTTAAAGGCTTAAGAAACAGTCTTTACCTAAGCATTATTCAAAATGAA	5	0.125	No Hit
TATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGCGTCTTTCAAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.025	0.0	0.0	0.0	0.0
16-17	0.05	0.0	0.0	0.0	0.0
18-19	0.05	0.0	0.0	0.0	0.0
20-21	0.075	0.0	0.0	0.0	0.0
22-23	0.075	0.0	0.0	0.0	0.0
24-25	0.075	0.0	0.0	0.0	0.0
26-27	0.0875	0.0	0.0	0.0	0.0
28-29	0.1	0.0	0.0	0.0	0.0
30-31	0.1	0.0	0.0	0.0	0.0
32-33	0.1	0.0	0.0	0.0	0.0
34-35	0.1	0.0	0.0	0.0	0.0
36-37	0.1	0.0	0.0	0.0	0.0
38-39	0.1	0.0	0.0	0.0	0.0
40-41	0.1	0.0	0.0	0.0	0.0
42-43	0.1	0.0	0.0	0.0	0.0
44-45	0.1	0.0	0.0	0.0	0.0
46-47	0.1	0.0	0.0	0.0	0.0
48-49	0.1	0.0	0.0	0.0	0.0
50-51	0.1	0.0	0.0	0.0	0.0
52-53	0.1	0.0	0.0	0.0	0.0
54-55	0.1	0.0	0.0	0.0	0.0
56-57	0.1	0.0	0.0	0.0	0.0
58-59	0.1	0.0	0.0	0.0	0.0
60-61	0.1	0.0	0.0	0.0	0.0
62-63	0.1	0.0	0.0	0.0	0.0
64-65	0.1	0.0	0.0	0.0	0.0
66-67	0.1	0.0	0.0	0.0	0.0
68-69	0.1	0.0	0.0	0.0	0.0
70-71	0.1	0.0	0.0	0.0	0.0
72-73	0.1	0.0	0.0	0.0	0.0
74-75	0.1	0.0	0.0	0.0	0.0
76-77	0.1	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.125	0.0	0.0	0.0	0.0
82-83	0.125	0.0	0.0	0.0	0.0
84-85	0.125	0.0	0.0	0.0	0.0
86-87	0.125	0.0	0.0	0.0	0.0
88-89	0.125	0.0	0.0	0.0	0.0
90-91	0.125	0.0	0.0	0.0	0.0
92-93	0.125	0.0	0.0	0.0	0.0
94-95	0.1375	0.0	0.0	0.0	0.0
96-97	0.15	0.0	0.0	0.0	0.0
98-99	0.15	0.0	0.0	0.0	0.0
100-101	0.15	0.0	0.0	0.0	0.0
102-103	0.15	0.0	0.0	0.0	0.0
104-105	0.15	0.0	0.0	0.0	0.0
106-107	0.15	0.0	0.0	0.0	0.0
108-109	0.15	0.0	0.0	0.0	0.0
110-111	0.15	0.0	0.0	0.0	0.0
112-113	0.15	0.0	0.0	0.0	0.0
114-115	0.15	0.0	0.0	0.0	0.0
116-117	0.15	0.0	0.0	0.0	0.0
118-119	0.16249999999999998	0.0	0.0	0.0	0.0
120-121	0.2	0.0	0.0	0.0	0.0
122-123	0.2	0.0	0.0	0.0	0.0
124-125	0.2	0.0	0.0	0.0	0.0
126-127	0.2	0.0	0.0	0.0	0.0
128-129	0.2	0.0	0.0	0.0	0.0
130-131	0.2	0.0	0.0	0.0	0.0
132-133	0.2	0.0	0.0	0.0	0.0
134-135	0.2	0.0	0.0	0.0	0.0
136-137	0.25	0.0	0.0	0.0	0.0
138	0.25	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
Read 2700190 spots for SRR25222513.sra
Written 2700190 spots for SRR25222513.sra
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
Read 2700183 spots for SRR25222513.sra
Written 2700183 spots for SRR25222513.sra
SRR ids: ['SRR25222513.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5hhh8s25
SRR25222513.sra spots: 21601471
blocks: [[1, 2700183], [2700184, 5400366], [5400367, 8100549], [8100550, 10800732], [10800733, 13500915], [13500916, 16201098], [16201099, 18901281], [18901282, 21601471]]
SRR25222513 file size 7930828
SRR25222513 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25222513 SRR25222513_1.fastq
Input file:	SRR25222513_1.fastq
trimmed:	SRR25222513-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Fri Jun 19 19:15:06 2026 >> started

Fri Jun 19 19:15:57 2026 >> done (51.119s)
21601471 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
21601471 (100.00%) reads available; of these:
     154 ( 0.00%) trimmed reads available after processing
21601317 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
143	       2	  0.00%
144	       0	  0.00%
145	       0	  0.00%
146	       6	  0.00%
147	      21	  0.00%
148	      60	  0.00%
149	      65	  0.00%
150	21601317	100.00%
21601471 reads passed initial QC


criterion=sequence-density
sequence-density=1.58
sequence-density-rank=1
fanout-score=1.94
fanout-score-rank=38
prefix-density=1.56
prefix-fanout=1.9
sequence=TACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGCGTCTTTCAAAACCATTATCATTTTCTCCAAGTACAATTGACTGGAATATCCATTGTAGTCTGTCAAATAAACTTCTTACAAGCATAAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=62.64
fanout-score-rank=1
prefix-density=0.24
prefix-fanout=4.0
sequence=AGAAGAAGAAGTGGTCCAAAGGCAAAGTTCGGGACAAGTTGAACAATCTTGTCCTGTTCGACAAAGCGACATACGACAAGCTCTGTAAGGAGGTTCCGAACTATAAGCTTATTACTCCAGCCGTGGTCTCTGAGAGACTGAAGATTCGCGGTTCCTTGGCCAGGGCAGCCCTTCAGGAGCTCCTTAGTAAAGGACTTATCAAGCTGGTTTCAAAGCACAGAGCCCAAGTAATTTACACCAGAAACACAAAGGGTGGGGACGCTCCAGCTGCTGGCGAAGATGCATGAACAGGTTCAATCAGCTGTACATTTGGAAAAATAAAACTTTATTGAATCA
                                 Started job on |	Jun 19 19:16:17
                             Started mapping on |	Jun 19 19:16:17
                                    Finished on |	Jun 19 19:17:41
       Mapping speed, Million of reads per hour |	925.78

                          Number of input reads |	21601471
                      Average input read length |	149
                                    UNIQUE READS:
                   Uniquely mapped reads number |	17969059
                        Uniquely mapped reads % |	83.18%
                          Average mapped length |	145.91
                       Number of splices: Total |	9632983
            Number of splices: Annotated (sjdb) |	9521397
                       Number of splices: GT/AG |	9558623
                       Number of splices: GC/AG |	20582
                       Number of splices: AT/AC |	2362
               Number of splices: Non-canonical |	51416
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.38
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.42
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1122036
             % of reads mapped to multiple loci |	5.19%
        Number of reads mapped to too many loci |	41137
             % of reads mapped to too many loci |	0.19%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.39%
                     % of reads unmapped: other |	0.04%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2510376	2510376	2510376
N_multimapping	1122036	1122036	1122036
N_noFeature	332644	2791833	15057103
N_ambiguous	2247624	1744059	68317
UnstrandedReadsAssigned:15388791 PositiveStrandReadsAssigned:13433167 NegativeStrandReadsAssigned:2843639
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25222513 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25222513-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 21,601,471 reads, 17,507,565 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,416 rounds

 109283 SRR25222513.ke.tsv
  52636 SRR25222513.se.tsv
 161919 total
==> SRR25222513.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25222513.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25222513 completed mapping pipeline successfully
