Starting /dee2/code/volunteer_pipeline.sh SRR25222519
    current disk space = 429165641728
    free memory = 391962092 
SRR25222519 SRAfilesize
ac332912a54a6b1d157fa09faaa41ff6  SRR25222519.sra
SRR25222519.sra file validated
SRR25222519 is single end
SRR25222519 is conventional basespace
SRR25222519 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25222519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.114	37.0	37.0	37.0	25.0	37.0
2	35.8335	37.0	37.0	37.0	37.0	37.0
3	35.713	37.0	37.0	37.0	37.0	37.0
4	36.0075	37.0	37.0	37.0	37.0	37.0
5	35.6615	37.0	37.0	37.0	37.0	37.0
6	34.981	37.0	37.0	37.0	25.0	37.0
7	35.159	37.0	37.0	37.0	25.0	37.0
8	35.844	37.0	37.0	37.0	37.0	37.0
9	35.807	37.0	37.0	37.0	37.0	37.0
10-14	35.318799999999996	37.0	37.0	37.0	32.2	37.0
15-19	35.2945	37.0	37.0	37.0	32.2	37.0
20-24	34.912699999999994	37.0	37.0	37.0	29.8	37.0
25-29	35.3779	37.0	37.0	37.0	32.2	37.0
30-34	35.1096	37.0	37.0	37.0	34.6	37.0
35-39	35.275	37.0	37.0	37.0	32.2	37.0
40-44	34.678700000000006	37.0	37.0	37.0	29.8	37.0
45-49	34.714999999999996	37.0	37.0	37.0	29.8	37.0
50-54	34.912	37.0	37.0	37.0	29.8	37.0
55-59	34.582300000000004	37.0	37.0	37.0	27.0	37.0
60-64	35.12140000000001	37.0	37.0	37.0	27.4	37.0
65-69	34.2547	37.0	37.0	37.0	25.0	37.0
70-74	33.8759	37.0	34.6	37.0	22.2	37.0
75-79	34.0288	37.0	37.0	37.0	22.2	37.0
80-84	34.5067	37.0	37.0	37.0	25.0	37.0
85-89	33.9719	37.0	37.0	37.0	22.2	37.0
90-94	33.5279	37.0	37.0	37.0	22.2	37.0
95-99	33.8792	37.0	37.0	37.0	25.0	37.0
100-104	32.4208	37.0	32.2	37.0	16.6	37.0
105-109	33.4766	37.0	34.6	37.0	22.2	37.0
110-114	32.908100000000005	37.0	34.6	37.0	16.6	37.0
115-119	32.979499999999994	37.0	37.0	37.0	13.8	37.0
120-124	32.59310000000001	37.0	32.2	37.0	19.4	37.0
125-129	33.3087	37.0	37.0	37.0	19.4	37.0
130-134	32.4656	37.0	32.2	37.0	11.0	37.0
135-139	32.2607	37.0	32.2	37.0	11.0	37.0
140-144	31.615	37.0	27.4	37.0	11.0	37.0
145-149	32.174699999999994	37.0	34.6	37.0	11.0	37.0
150	32.8175	37.0	37.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	0.0
16	4.0
17	2.0
18	3.0
19	2.0
20	2.0
21	7.0
22	10.0
23	15.0
24	17.0
25	24.0
26	41.0
27	43.0
28	73.0
29	88.0
30	139.0
31	194.0
32	358.0
33	434.0
34	668.0
35	883.0
36	967.0
37	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.1	16.45	10.75	32.7
2	15.075	21.05	33.650000000000006	30.225
3	14.549999999999999	24.425	29.175	31.85
4	10.549999999999999	15.85	33.25	40.35
5	11.175	38.35	33.4	17.075000000000003
6	27.224999999999998	36.575	18.05	18.15
7	22.925	32.800000000000004	21.95	22.325
8	21.275	38.15	18.85	21.725
9	21.5	21.025	17.675	39.800000000000004
10-14	19.900000000000002	24.104999999999997	29.065	26.93
15-19	19.645000000000003	30.12	24.195	26.040000000000003
20-24	20.849999999999998	26.985	26.450000000000003	25.715
25-29	21.240000000000002	28.17	25.605	24.985
30-34	20.1	29.385	25.575	24.94
35-39	20.43	28.134999999999998	25.905	25.53
40-44	19.97	30.335	25.97	23.724999999999998
45-49	19.900000000000002	29.49	25.629999999999995	24.98
50-54	19.395	29.805	26.735	24.065
55-59	19.015	30.159999999999997	26.200000000000003	24.625
60-64	19.235	30.64	25.47	24.654999999999998
65-69	19.015	32.445	25.15	23.39
70-74	18.87	31.525	25.94	23.665
75-79	19.515	32.43	25.840000000000003	22.215
80-84	19.86	33.14	24.025	22.975
85-89	19.040000000000003	33.715	24.87	22.375
90-94	18.625	34.165	25.105	22.105
95-99	19.02	33.77	24.385	22.825
100-104	17.89	34.285	25.16	22.665
105-109	18.605	35.295	25.255	20.845
110-114	18.154999999999998	36.1	24.535	21.21
115-119	18.17	35.79	24.44	21.6
120-124	17.155	39.300000000000004	22.405	21.14
125-129	17.195	38.685	22.24	21.88
130-134	17.525	39.085	22.869999999999997	20.52
135-139	17.765	39.485	22.865	19.885
140-144	17.935000000000002	40.644999999999996	21.465	19.955000000000002
145-149	17.8	37.815	24.104999999999997	20.28
150	19.525000000000002	37.375	24.825	18.275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	2.0
3	1.5
4	1.5
5	2.5
6	5.0
7	6.0
8	2.0
9	3.0
10	4.5
11	2.5
12	4.0
13	6.0
14	8.5
15	9.0
16	8.5
17	6.5
18	5.5
19	7.5
20	9.5
21	11.5
22	11.0
23	14.0
24	17.5
25	20.5
26	26.5
27	27.0
28	22.0
29	19.0
30	33.0
31	47.5
32	50.0
33	59.0
34	77.5
35	94.5
36	118.5
37	136.0
38	122.0
39	130.5
40	138.5
41	111.5
42	102.0
43	101.5
44	124.5
45	153.0
46	220.5
47	277.5
48	214.0
49	181.0
50	189.0
51	140.5
52	101.0
53	91.5
54	105.5
55	103.5
56	69.5
57	70.0
58	81.0
59	68.5
60	54.5
61	43.5
62	29.0
63	21.0
64	17.0
65	11.5
66	5.5
67	6.0
68	7.5
69	5.5
70	3.0
71	1.5
72	1.5
73	0.5
74	1.0
75	1.0
76	0.0
77	0.5
78	1.5
79	1.0
80	0.0
81	0.5
82	0.5
83	0.5
84	0.5
85	1.5
86	2.0
87	1.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.23420074349443	55.974999999999994
2	9.516728624535316	12.8
3	2.0446096654275094	4.125
4	1.5241635687732342	4.1000000000000005
5	1.3011152416356877	4.375
6	0.5204460966542751	2.1
7	0.37174721189591076	1.7500000000000002
8	0.18587360594795538	1.0
9	0.29739776951672864	1.7999999999999998
>10	0.966542750929368	10.7
>50	0.03717472118959108	1.275
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCCATAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGT	51	1.275	No Hit
TCCTACCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAA	44	1.0999999999999999	No Hit
GTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTAC	42	1.05	No Hit
ATTTTCAAATTATTCACAGCGCTGCCAAGTTCACCAAGAAGTGACTCTGA	30	0.75	No Hit
GTGCTACTATCATCCACACATTTCTACCTGAGACCACCCAAAGGACCCTC	20	0.5	No Hit
TCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGCATTCCTC	20	0.5	No Hit
GGTCCGCTGTGTTCAACGTTTCTAGAATGCTGTTCTTGGCTAATGGCCAA	18	0.44999999999999996	No Hit
GACCAGTACTCTACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCT	18	0.44999999999999996	No Hit
GAACCAAGGATGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCAT	17	0.42500000000000004	No Hit
CTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAAT	16	0.4	No Hit
CTCCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCT	16	0.4	No Hit
GAATAAAATCAACCAAAGCACTGTGGAATTTTGGGAGAAATAACCTTTAA	15	0.375	No Hit
CTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTACTGCTAGAGAT	15	0.375	No Hit
GTTCCCAATGAGCAGGCCACACAAGACAACAAGGCTCCCAAGTTGGAACA	14	0.35000000000000003	No Hit
CACAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTTTGAAG	13	0.325	No Hit
GGTACAACTTGCCCTCTGAAGAGGAAGAACAGAAGGATGCCACAACTCTC	12	0.3	No Hit
CTCTAATCTACTGGCAGGACAGGTACAACTTGCCCTCTGAAGAGGAAGAA	12	0.3	No Hit
CAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCT	12	0.3	No Hit
CAACAATAAACCATTCAAAGATGTAAAGCTTTTTAAATTGCCAAGGTCCG	12	0.3	No Hit
GCGCTGCCAAGTTCACCAAGAAGTGACTCTGAGTTCCCAATGAGCAGGCC	11	0.27499999999999997	No Hit
CATTTCTACCTGAGACCACCCAAAGGACCCTCCCAAATTCTCTTCCTCTC	11	0.27499999999999997	No Hit
TAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGG	10	0.25	No Hit
AGCAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTACTGCTA	10	0.25	No Hit
GGTCAGAAGCGGTCTGGTCAGTTATTTTTGAGGGGTGGAAGACCACACCC	10	0.25	No Hit
GACCAGTACTATACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCT	10	0.25	No Hit
CAACTACAATGCCAGCTGCCAGTATCTCCATAACTACTCCTACTGCTAGA	10	0.25	No Hit
CCAAAGCACTGTGGAATTTTGGGAGAAATAACCTTTAAAGGCTTAAGAAA	10	0.25	No Hit
GAACAGAAGGATGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCA	9	0.22499999999999998	No Hit
GTCTTTACCTAAGCATTATTCAAAATGAAAATAAAGAGCTGAGCAGCATT	9	0.22499999999999998	No Hit
GAACCAAGGAGGCTTCCCCAGCCAAGGGCACCGTCAGATCAATGCCATTG	9	0.22499999999999998	No Hit
CCTCCACCTACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACA	9	0.22499999999999998	No Hit
TCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCTACTATCATCCACAC	9	0.22499999999999998	No Hit
CCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGCATTCCTCCTACCGG	9	0.22499999999999998	No Hit
CCTCTAGAAAGTGCTACTATCATCCACACATTTCTACCTGAGACCACCCA	9	0.22499999999999998	No Hit
CCTCCTACCGGACCTCCTACCACAGTACAAGCAACTACAATGCCAGCTGC	9	0.22499999999999998	No Hit
CCTCTCTAATCTACTGGCAGGACAGGTACAACTTGCCCTCTGAAGAGGAA	8	0.2	No Hit
CTTATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGCGTCTTTCA	8	0.2	No Hit
TGCCACAACTCTCCTGCTGGCTACTCTCCAGTGGTTTCATCTTACTTCTG	8	0.2	No Hit
CCTTACAAGTACATTGTCAACCGTCCTGCAAAACTTCCTATGTCCATTGC	8	0.2	No Hit
CTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTA	8	0.2	No Hit
CTCCACTCTGCTCGCTGCCATCGTGGCAGTAACTGCTTGTCTCCCCGTGC	7	0.17500000000000002	No Hit
CCCCCTCCACCTCCTTGTCCACCAGTTCCTCCACATCCTAGACCTCCAAG	7	0.17500000000000002	No Hit
GAATTTTCAAATTATTCACAGCGCTGCCAAGTTCACCAAGAAGTGACTCT	7	0.17500000000000002	No Hit
ACCTGAGACCACCCAAAGGACCCTCCCAAATTCTCTTCCTCTCTGAGTAG	7	0.17500000000000002	No Hit
CTCCACCTCCTTGTCCACCAGTTCCTCCACATCCTAGACCTCCAAGCAAT	7	0.17500000000000002	No Hit
GCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAATTAATAAATTC	7	0.17500000000000002	No Hit
ACCAATTCCTCCACATCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTA	7	0.17500000000000002	No Hit
ACAATAAACCATTCAAAGATGTAAAGCTTTTTAAATTGCCAAGGTCCGCT	7	0.17500000000000002	No Hit
GCAACTCCACATCCAGATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGA	7	0.17500000000000002	No Hit
GTCCTCAGCACACTGAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCT	7	0.17500000000000002	No Hit
GCACCGTCAGATCAATGCCATTGCCGTTGGAAGACAGCTTAGCTTGAAAA	6	0.15	No Hit
CCTCCTACCACAGTACAAGCAACTACAATGCCAGCTGCCAGTATCTCCAT	6	0.15	No Hit
GGTTTGCTTTGAAGAAGGATGCACACGAGAGAGATCCTTGTTTCTCTGCA	6	0.15	No Hit
CCCTTTGGTCCAGGAATTGGTAGACCACCCCCTCCACCCTTTGGTCCAGG	6	0.15	No Hit
CTCCACATCCTAGACCTCCAAGCAATCCGTCTCCTCCACCTACTCCAAGC	6	0.15	No Hit
ACTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTAC	6	0.15	No Hit
GAGTTCCCAATGAGCAGGCCACACAAGACAACAAGGCTCCCAAGTTGGAA	6	0.15	No Hit
TCTTCAGGGTCTCCTCTCTAATCTACTGGCAGGACAGGTACAACTTGCCC	6	0.15	No Hit
AGACTACACCCTCCACCCTTTGGTCCAGGAATTGGTAGACCACCCCCTCC	6	0.15	No Hit
CCCAAAGGACCCTCCCAAATTCTCTTCCTCTCTGAGTAGTCTCCACACCT	6	0.15	No Hit
GCTAGGATTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA	6	0.15	No Hit
TTATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGCGTCTTTCAA	6	0.15	No Hit
GCATTTCCCTCTAGAAAGTGCTACTATCATCCACACATTTCTACCTGAGA	6	0.15	No Hit
CTACTCCTACTGCTAGAGATTCCACTGATATTTTTTGGAGGTTGTGGGAA	6	0.15	No Hit
GTCCAGGAATTGGTCGACCACCCCCTCCACCTCCTTGTCCACCAGTTCCT	5	0.125	No Hit
CCCCAGCCAAGGGCACCGTCAGATCAATGCCATTGCCGTTGGAAGACAGC	5	0.125	No Hit
GGCAGGACAGGTACAACTTGCCCTCTGAAGAGGAAGAACAGAAGGATGCC	5	0.125	No Hit
CAGTATCTCCATAACTACTCCTACTGCTAGAGATTCCACTGATATTTTTT	5	0.125	No Hit
CCTCAGCACACTGAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCTAC	5	0.125	No Hit
CTCCAAGCATTCCTCCTACCGGACCTCCTACCACAGTACAAGCAACTACA	5	0.125	No Hit
CCCAAATTCTCTTCCTCTCTGAGTAGTCTCCACACCTGTTACCACCATCC	5	0.125	No Hit
CCCTCCACCTCCTTGTCCACCAGTTCCTCCACATCCTAGACCTCCAAGCA	5	0.125	No Hit
CCTCTACCCTGTGGCAGATGACAGAAATGCTTTTTGGCAGTGCATCAATG	5	0.125	No Hit
CATCCAGATTCAGGTTCTGAGGGACAGCTTCAGAGGGAGGATTCAGAATT	5	0.125	No Hit
ATTTAAGGTTGCTTTGAAATTGAATATGAACCACTTCCTTGAAGGATCAA	5	0.125	No Hit
GTAGAGACACCATCCAGAATACTGTTGATGATGGGTAATCGTCTTCCCAA	5	0.125	No Hit
GAATCCAAGTGTTCTTCAGGGTCTCCTCTCTAATCTACTGGCAGGACAGG	5	0.125	No Hit
GAGGGAGGATTCAGAATTTTCAAATTATTCACAGCGCTGCCAAGTTCACC	5	0.125	No Hit
GTTGTTAACAATGGCTATGATTACAATCATTTAGATCTATGATTTGGGCT	5	0.125	No Hit
GGCACATACTGTGATGTCATTTCTGGAGATAAAGTCGATGGCAATTGCAC	5	0.125	No Hit
GACAGGTACAACTTGCCCTCTGAAGAGGAAGAACAGAAGGATGCCACAAC	5	0.125	No Hit
CCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAAGTGCTAC	5	0.125	No Hit
GCATTAACCCAGACAGCACTTGTGGCAATGACTGGATCTGTGAACATCGA	5	0.125	No Hit
GCCAGTATCTCCATAACTACTCCTACTGCTAGAGATTCCACTGATATTTT	5	0.125	No Hit
CTGAAGAGGAAGAACAGAAGGATGCCACAACTCTCCTGCTGGCTACTCTC	5	0.125	No Hit
CTCCTGCTGGCTACTCTCCAGTGGTTTCATCTTACTTCTGATGGCATTTC	5	0.125	No Hit
CAGTATTCTGGATGGTGTCTCTACCCTCCTTACAAGTACATTGTCAACCG	5	0.125	No Hit
TTCCTGAGGTGACCATAGGCAAATGCTCCAGTAATACAGATAAAATCTCC	5	0.125	No Hit
GGCCACACAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTT	5	0.125	No Hit
GGGTGGTCTCCACACCTGTTACCACCATCCCAGAATTAAAATCCTAACTG	5	0.125	No Hit
TCCTATGTCCATTGCTTCAGTACGTCCTCAGCACACTGAATCCAAGTGTT	5	0.125	No Hit
CCTCTGAAGAGGAAGAACAGAAGGATGCCACAACTCTCCTGCTGGCTACT	5	0.125	No Hit
ACCCAAAGGACCCTCCCAAATTCTCTTCCTCTCTGAGTAGTCTCCACACC	5	0.125	No Hit
CAAGACAACAAGGCTCCCAAGTTGGAACATCTTTGGTTTGCTTTGAAGAA	5	0.125	No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG	5	0.125	No Hit
GTTCACCAAGAAGTGACTCTGAGTTCCCAATGAGCAGGCCACACAAGACA	5	0.125	No Hit
GATAATGACAGCAACCAGGTAGCTTTTGGCAGAGGAAACAAAGGATTCAT	5	0.125	No Hit
ACTCTCCAGTGGTTTCATCTTACTTCTGATGGCATTTCCCTCTAGAAAGT	5	0.125	No Hit
CCTTTAAAGGCTTAAGAAACAGTCTTTACCTAAGCATTATTCAAAATGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0125	0.0	0.0	0.0	0.0
14-15	0.05	0.0	0.0	0.0	0.0
16-17	0.1	0.0	0.0	0.0	0.0
18-19	0.125	0.0	0.0	0.0	0.0
20-21	0.125	0.0	0.0	0.0	0.0
22-23	0.125	0.0	0.0	0.0	0.0
24-25	0.125	0.0	0.0	0.0	0.0
26-27	0.125	0.0	0.0	0.0	0.0
28-29	0.1375	0.0	0.0	0.0	0.0
30-31	0.15	0.0	0.0	0.0	0.0
32-33	0.15	0.0	0.0	0.0	0.0
34-35	0.15	0.0	0.0	0.0	0.0
36-37	0.15	0.0	0.0	0.0	0.0
38-39	0.16249999999999998	0.0	0.0	0.0	0.0
40-41	0.175	0.0	0.0	0.0	0.0
42-43	0.175	0.0	0.0	0.0	0.0
44-45	0.175	0.0	0.0	0.0	0.0
46-47	0.175	0.0	0.0	0.0	0.0
48-49	0.175	0.0	0.0	0.0	0.0
50-51	0.175	0.0	0.0	0.0	0.0
52-53	0.175	0.0	0.0	0.0	0.0
54-55	0.175	0.0	0.0	0.0	0.0
56-57	0.175	0.0	0.0	0.0	0.0
58-59	0.175	0.0	0.0	0.0	0.0
60-61	0.175	0.0	0.0	0.0	0.0
62-63	0.175	0.0	0.0	0.0	0.0
64-65	0.175	0.0	0.0	0.0	0.0
66-67	0.175	0.0	0.0	0.0	0.0
68-69	0.175	0.0	0.0	0.0	0.0
70-71	0.175	0.0	0.0	0.0	0.0
72-73	0.175	0.0	0.0	0.0	0.0
74-75	0.175	0.0	0.0	0.0	0.0
76-77	0.175	0.0	0.0	0.0	0.0
78-79	0.175	0.0	0.0	0.0	0.0
80-81	0.175	0.0	0.0	0.0	0.0
82-83	0.175	0.0	0.0	0.0	0.0
84-85	0.175	0.0	0.0	0.0	0.0
86-87	0.175	0.0	0.0	0.0	0.0
88-89	0.175	0.0	0.0	0.0	0.0
90-91	0.175	0.0	0.0	0.0	0.0
92-93	0.175	0.0	0.0	0.0	0.0
94-95	0.175	0.0	0.0	0.0	0.0
96-97	0.175	0.0	0.0	0.0	0.0
98-99	0.175	0.0	0.0	0.0	0.0
100-101	0.175	0.0	0.0	0.0	0.0
102-103	0.175	0.0	0.0	0.0	0.0
104-105	0.175	0.0	0.0	0.0	0.0
106-107	0.175	0.0	0.0	0.0	0.0
108-109	0.175	0.0	0.0	0.0	0.0
110-111	0.175	0.0	0.0	0.0	0.0
112-113	0.1875	0.0	0.0	0.0	0.0
114-115	0.2	0.0	0.0	0.0	0.0
116-117	0.2	0.0	0.0	0.0	0.0
118-119	0.2	0.0	0.0	0.0	0.0
120-121	0.2	0.0	0.0	0.0	0.0
122-123	0.2	0.0	0.0	0.0	0.0
124-125	0.2	0.0	0.0	0.0	0.0
126-127	0.2	0.0	0.0	0.0	0.0
128-129	0.2	0.0	0.0	0.0	0.0
130-131	0.2	0.0	0.0	0.0	0.0
132-133	0.2	0.0	0.0	0.0	0.0
134-135	0.25	0.0	0.0	0.0	0.0
136-137	0.25	0.0	0.0	0.0	0.0
138	0.25	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATTC	10	0.006973645	144.0	2
CAGTACT	10	0.006973645	144.0	4
ACCAATT	10	0.006973645	144.0	1
CAATTCC	10	0.006973645	144.0	3
ACTCTAC	10	0.006973645	144.0	8
CTCTACT	10	0.006973645	144.0	9
ATTACCT	10	0.006973645	144.0	4
ACCAGTA	10	0.006973645	144.0	2
AAAATCA	35	0.0034045284	61.714283	5
ATCAACC	35	0.0034045284	61.714283	8
AAATCAA	40	0.005777437	54.0	6
AATCAAC	40	0.005777437	54.0	7
TCAACCA	40	0.005777437	54.0	9
ATAAAAT	45	0.009205684	48.0	3
>>END_MODULE
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944035 spots for SRR25222519.sra
Written 944035 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
Read 944029 spots for SRR25222519.sra
Written 944029 spots for SRR25222519.sra
SRR ids: ['SRR25222519.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ltbso27g
SRR25222519.sra spots: 15104470
blocks: [[1, 944029], [944030, 1888058], [1888059, 2832087], [2832088, 3776116], [3776117, 4720145], [4720146, 5664174], [5664175, 6608203], [6608204, 7552232], [7552233, 8496261], [8496262, 9440290], [9440291, 10384319], [10384320, 11328348], [11328349, 12272377], [12272378, 13216406], [13216407, 14160435], [14160436, 15104470]]
SRR25222519 file size 2007031
SRR25222519 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25222519 SRR25222519_1.fastq
Input file:	SRR25222519_1.fastq
trimmed:	SRR25222519-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Fri Jun 19 17:29:25 2026 >> started

Fri Jun 19 17:29:48 2026 >> done (22.338s)
15104470 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
15104470 (100.00%) reads available; of these:
     107 ( 0.00%) trimmed reads available after processing
15104363 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
146	       8	  0.00%
147	      19	  0.00%
148	      42	  0.00%
149	      38	  0.00%
150	15104363	100.00%
15104470 reads passed initial QC


criterion=sequence-density
sequence-density=1.73
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=28
prefix-density=1.72
prefix-fanout=2.0
sequence=TACTTATAATACTGTGCGAATGAATTAACGGAGGCTTCTTATGCGTCTTTCAAAACCATTATCATTTTCTCCAAGTACAATTGACTGGAATATCCATTGTAGTCTGTCAAATAAACTTCTTACAAGCATAAAAA


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=22
fanout-score=123.05
fanout-score-rank=1
prefix-density=6.72
prefix-fanout=1.0
sequence=AATTAAAATCCAACTTATATGTGAAAATTCATTGTTAGGACCTAAACTCAATAACGAAAGTAATTCTAGTCATTTATAATACACGACAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACCTAAATAATTAAATTTAACAAAACTATTTGCCAGAGAACTACTAGCCATAGCTTAAAACTCAAAGGACTTGGCGGTACTTTATATCCATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCATCTCTTGCTAATTCAGCCTATATACCGCCATCTTCAGCAAACCCTAAAAAGGTATTAAAGTAAGCAAAAGAATCAAACATAAAAACGTTAGGTCAA
                                 Started job on |	Jun 19 17:30:24
                             Started mapping on |	Jun 19 17:30:24
                                    Finished on |	Jun 19 17:31:50
       Mapping speed, Million of reads per hour |	632.28

                          Number of input reads |	15104470
                      Average input read length |	149
                                    UNIQUE READS:
                   Uniquely mapped reads number |	12449040
                        Uniquely mapped reads % |	82.42%
                          Average mapped length |	145.67
                       Number of splices: Total |	6208796
            Number of splices: Annotated (sjdb) |	6130014
                       Number of splices: GT/AG |	6158689
                       Number of splices: GC/AG |	13908
                       Number of splices: AT/AC |	1514
               Number of splices: Non-canonical |	34685
                      Mismatch rate per base, % |	0.48%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.39
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.38
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	753901
             % of reads mapped to multiple loci |	4.99%
        Number of reads mapped to too many loci |	32557
             % of reads mapped to too many loci |	0.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.33%
                     % of reads unmapped: other |	0.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1901529	1901529	1901529
N_multimapping	753901	753901	753901
N_noFeature	268481	1677203	10764549
N_ambiguous	1683631	1368643	58641
UnstrandedReadsAssigned:10496928 PositiveStrandReadsAssigned:9403194 NegativeStrandReadsAssigned:1625850
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25222519 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25222519-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 15,104,470 reads, 10,328,870 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,022 rounds

 109283 SRR25222519.ke.tsv
  52636 SRR25222519.se.tsv
 161919 total
==> SRR25222519.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25222519.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25222519 completed mapping pipeline successfully
