Starting /dee2/code/volunteer_pipeline.sh SRR25257915
    current disk space = 548959528
    free memory = 196008840 
SRR25257915 SRAfilesize
38386c3dc727a92a4a75580f96da3d67  SRR25257915.sra
SRR25257915.sra file validated
SRR25257915 is paired end
SRR25257915 is conventional basespace
SRR25257915 read1 length is 53-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257915_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	53-151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.594	37.0	37.0	37.0	37.0	37.0
2	36.49925	37.0	37.0	37.0	37.0	37.0
3	36.5785	37.0	37.0	37.0	37.0	37.0
4	36.674	37.0	37.0	37.0	37.0	37.0
5	36.581	37.0	37.0	37.0	37.0	37.0
6	36.5725	37.0	37.0	37.0	37.0	37.0
7	36.558	37.0	37.0	37.0	37.0	37.0
8	36.535	37.0	37.0	37.0	37.0	37.0
9	36.57	37.0	37.0	37.0	37.0	37.0
10-14	36.6044	37.0	37.0	37.0	37.0	37.0
15-19	36.523700000000005	37.0	37.0	37.0	37.0	37.0
20-24	36.5209	37.0	37.0	37.0	37.0	37.0
25-29	36.46665	37.0	37.0	37.0	37.0	37.0
30-34	36.33455	37.0	37.0	37.0	37.0	37.0
35-39	36.4062	37.0	37.0	37.0	37.0	37.0
40-44	36.475300000000004	37.0	37.0	37.0	37.0	37.0
45-49	36.35744999999999	37.0	37.0	37.0	37.0	37.0
50-54	36.21174909954978	37.0	37.0	37.0	37.0	37.0
55-59	36.15608237180596	37.0	37.0	37.0	37.0	37.0
60-64	36.27700468814643	37.0	37.0	37.0	37.0	37.0
65-69	36.32829226425278	37.0	37.0	37.0	37.0	37.0
70-74	36.2535058094391	37.0	37.0	37.0	37.0	37.0
75-79	36.16747336288559	37.0	37.0	37.0	37.0	37.0
80-84	36.24124744465547	37.0	37.0	37.0	37.0	37.0
85-89	36.22795416785287	37.0	37.0	37.0	37.0	37.0
90-94	36.1889487869599	37.0	37.0	37.0	37.0	37.0
95-99	36.070916636149185	37.0	37.0	37.0	37.0	37.0
100-104	35.91499786388151	37.0	37.0	37.0	37.0	37.0
105-109	36.13152624930342	37.0	37.0	37.0	37.0	37.0
110-114	35.99627269882069	37.0	37.0	37.0	37.0	37.0
115-119	35.82519036916691	37.0	37.0	37.0	37.0	37.0
120-124	36.00368618734569	37.0	37.0	37.0	37.0	37.0
125-129	35.881866399651344	37.0	37.0	37.0	37.0	37.0
130-134	35.71315472865673	37.0	37.0	37.0	37.0	37.0
135-139	35.50323936599494	37.0	37.0	37.0	34.6	37.0
140-144	35.825194420358116	37.0	37.0	37.0	37.0	37.0
145-149	35.57071302296188	37.0	37.0	37.0	37.0	37.0
150-151	35.03474720154858	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	4.0
27	6.0
28	7.0
29	14.0
30	25.0
31	36.0
32	33.0
33	57.0
34	92.0
35	444.0
36	2924.0
37	356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.975	13.125	18.7	43.2
2	18.72968242060515	20.005001250312578	40.985246311577896	20.280070017504375
3	22.25	21.625	31.2	24.925
4	19.925	32.300000000000004	28.349999999999998	19.425
5	22.55	34.075	29.599999999999998	13.775
6	16.625	32.074999999999996	35.075	16.225
7	13.225000000000001	20.7	49.425000000000004	16.650000000000002
8	18.099999999999998	24.25	35.0	22.650000000000002
9	17.025000000000002	21.45	40.75	20.775
10-14	19.759999999999998	26.515	30.865	22.86
15-19	19.139999999999997	27.445000000000004	31.974999999999998	21.44
20-24	19.43	26.75	31.15	22.67
25-29	18.52426989931373	25.607373641236286	31.202725041326453	24.665631418123528
30-34	20.155661561636958	26.94451418528747	30.223449661059504	22.676374592016067
35-39	19.387652836239727	25.185407897374223	31.94527961515334	23.48165965123271
40-44	20.369999999999997	24.595	31.180000000000003	23.855
45-49	18.709547940394362	25.543123777030758	31.82479554462897	23.922532737945915
50-54	19.864491844416563	26.715181932245923	30.52948557089084	22.890840652446677
55-59	20.384731594319668	25.420485446671364	30.874206868768255	23.320576090240706
60-64	22.297364993487626	27.166616571485825	29.115319106301975	21.420699328724577
65-69	21.38128197411977	26.2965192095496	30.06821145551209	22.253987360818535
70-74	21.06957790411028	26.0200231423253	30.386879307742614	22.523519645821803
75-79	21.47889108509452	25.629719730373523	30.87527241396787	22.016116770564086
80-84	20.137544574630667	26.16403464085583	30.79979623025981	22.898624554253693
85-89	20.833117723156533	25.319534282018115	30.664941785252264	23.18240620957309
90-94	23.209941829719725	26.31411951348493	28.878900052882074	21.597038603913273
95-99	20.12375424378491	25.939108531376633	30.210272697404445	23.726864527434017
100-104	20.627081658435742	25.106236361548177	30.733892270586882	23.532789709429196
105-109	22.778277827782777	25.610561056105613	28.5988598859886	23.01230123012301
110-114	20.57288351368555	26.549968173138126	29.261616804583067	23.61553150859325
115-119	22.770515365135857	27.588802956676478	28.245842173704744	21.394839504482924
120-124	21.212348269144314	25.21354713022628	31.192866776562266	22.381237824067135
125-129	19.731925264012997	27.140536149471973	30.34118602761982	22.786352558895206
130-134	20.046246887228744	26.94770544290288	30.291711134827466	22.71433653504091
135-139	20.620669464233433	27.178686444813117	28.623011613155068	23.577632477798378
140-144	20.721485411140584	25.761273209549074	29.177718832891248	24.339522546419097
145-149	21.674051011854868	27.51766255538259	28.1044186324991	22.703867800263442
150-151	21.229586935638807	26.12872238232469	29.394812680115272	23.246878001921228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.5
21	1.5
22	0.5
23	7.0
24	11.0
25	20.0
26	39.5
27	51.0
28	57.5
29	70.5
30	72.0
31	64.0
32	78.0
33	93.5
34	98.5
35	92.5
36	104.0
37	136.5
38	139.0
39	159.5
40	185.0
41	193.5
42	211.0
43	238.0
44	227.5
45	179.5
46	190.0
47	198.5
48	158.5
49	106.5
50	92.5
51	111.5
52	89.0
53	77.0
54	76.5
55	53.0
56	39.0
57	34.0
58	35.0
59	42.5
60	46.0
61	24.0
62	10.0
63	18.5
64	17.0
65	8.0
66	6.0
67	21.0
68	27.0
69	13.0
70	8.5
71	12.0
72	8.5
73	3.0
74	1.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.185
30-34	0.42500000000000004
35-39	0.22
40-44	0.0
45-49	0.345
50-54	0.36503650365036505
55-59	0.5807549814759186
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.1720212496837845
80-84	0.0
85-89	0.149839826392477
90-94	0.11092916380539856
95-99	0.3709765411893072
100-104	0.6560556791602488
105-109	0.12585400934915497
110-114	0.21595528455284554
115-119	0.5986801823253283
120-124	0.08235382196601033
125-129	0.2754374594944912
130-134	0.583554376657825
135-139	1.1575190508343782
140-144	0.28565382987727467
145-149	1.113084665482534
150-151	0.5097164702134438
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52-53	2.0
54-55	2.0
56-57	2.0
58-59	0.0
60-61	2.0
62-63	1.0
64-65	1.0
66-67	4.0
68-69	3.0
70-71	8.0
72-73	7.0
74-75	10.0
76-77	10.0
78-79	8.0
80-81	13.0
82-83	19.0
84-85	24.0
86-87	24.0
88-89	36.0
90-91	36.0
92-93	46.0
94-95	45.0
96-97	63.0
98-99	64.0
100-101	59.0
102-103	68.0
104-105	70.0
106-107	72.0
108-109	63.0
110-111	95.0
112-113	77.0
114-115	70.0
116-117	109.0
118-119	122.0
120-121	90.0
122-123	92.0
124-125	76.0
126-127	73.0
128-129	81.0
130-131	101.0
132-133	72.0
134-135	61.0
136-137	80.0
138-139	73.0
140-141	67.0
142-143	99.0
144-145	76.0
146-147	71.0
148-149	70.0
150-151	1583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.65
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.788608981380065	27.750000000000004
2	17.14129244249726	15.65
3	7.338444687842278	10.05
4	4.326396495071194	7.9
5	3.285870755750274	7.5
6	2.135815991237678	5.8500000000000005
7	1.2595837897042717	4.025
8	0.547645125958379	2.0
9	0.8762322015334063	3.5999999999999996
>10	2.3001095290251916	15.675
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	28	0.7000000000000001	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	27	0.675	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	24	0.6	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	24	0.6	No Hit
AGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCA	22	0.5499999999999999	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	22	0.5499999999999999	No Hit
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	19	0.475	No Hit
CACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGC	18	0.44999999999999996	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	18	0.44999999999999996	No Hit
CTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAAT	18	0.44999999999999996	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	17	0.42500000000000004	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	16	0.4	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	16	0.4	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	16	0.4	No Hit
AATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCAT	16	0.4	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	15	0.375	No Hit
CTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAA	15	0.375	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	15	0.375	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	14	0.35000000000000003	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	14	0.35000000000000003	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	13	0.325	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	13	0.325	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	13	0.325	No Hit
GTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGG	13	0.325	No Hit
CTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGA	13	0.325	No Hit
GTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGT	13	0.325	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	12	0.3	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	12	0.3	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	12	0.3	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	12	0.3	No Hit
CTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCT	12	0.3	No Hit
CAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTG	12	0.3	No Hit
GTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCT	11	0.27499999999999997	No Hit
CGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCT	11	0.27499999999999997	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	11	0.27499999999999997	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	10	0.25	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	10	0.25	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	10	0.25	No Hit
GGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATG	10	0.25	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	10	0.25	No Hit
CTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCC	10	0.25	No Hit
CTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCC	10	0.25	No Hit
TTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAG	9	0.22499999999999998	No Hit
ATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCA	9	0.22499999999999998	No Hit
CTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTG	9	0.22499999999999998	No Hit
ACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATA	9	0.22499999999999998	No Hit
CCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGA	9	0.22499999999999998	No Hit
GTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCT	9	0.22499999999999998	No Hit
CTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTT	9	0.22499999999999998	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	9	0.22499999999999998	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	9	0.22499999999999998	No Hit
GTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGC	9	0.22499999999999998	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	9	0.22499999999999998	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	9	0.22499999999999998	No Hit
CCACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATT	9	0.22499999999999998	No Hit
CTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTC	9	0.22499999999999998	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	9	0.22499999999999998	No Hit
ATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGC	9	0.22499999999999998	No Hit
TAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTT	8	0.2	No Hit
TTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAAT	8	0.2	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	8	0.2	No Hit
CATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGA	8	0.2	No Hit
TAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCA	8	0.2	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	8	0.2	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	8	0.2	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	8	0.2	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	8	0.2	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	8	0.2	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	7	0.17500000000000002	No Hit
CTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCA	7	0.17500000000000002	No Hit
ATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTTTCT	7	0.17500000000000002	No Hit
CTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCA	7	0.17500000000000002	No Hit
CAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGA	7	0.17500000000000002	No Hit
TTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCA	7	0.17500000000000002	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	7	0.17500000000000002	No Hit
GCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTG	7	0.17500000000000002	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	7	0.17500000000000002	No Hit
CCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATC	7	0.17500000000000002	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	7	0.17500000000000002	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	7	0.17500000000000002	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	7	0.17500000000000002	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	7	0.17500000000000002	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	7	0.17500000000000002	No Hit
CTCATCCTTTCTGCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTG	7	0.17500000000000002	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	7	0.17500000000000002	No Hit
CTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGCAAATA	7	0.17500000000000002	No Hit
CTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAG	7	0.17500000000000002	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	7	0.17500000000000002	No Hit
GGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTG	7	0.17500000000000002	No Hit
CTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTGC	7	0.17500000000000002	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	7	0.17500000000000002	No Hit
CTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGT	6	0.15	No Hit
TGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTC	6	0.15	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	6	0.15	No Hit
CCGGGTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTAC	6	0.15	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	6	0.15	No Hit
ATGAGATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCT	6	0.15	No Hit
GGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAG	6	0.15	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	6	0.15	No Hit
GCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCC	6	0.15	No Hit
CAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGAGC	6	0.15	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	6	0.15	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	6	0.15	No Hit
TCTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGG	6	0.15	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	6	0.15	No Hit
CACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATG	6	0.15	No Hit
CTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAAATA	6	0.15	No Hit
CTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACAT	6	0.15	No Hit
GGAAAAAAAGCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCA	6	0.15	No Hit
ATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTT	6	0.15	No Hit
TGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAAT	6	0.15	No Hit
GAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAGGT	6	0.15	No Hit
AGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGT	6	0.15	No Hit
GAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTT	6	0.15	No Hit
GAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAA	6	0.15	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	6	0.15	No Hit
GTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCC	6	0.15	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	6	0.15	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	6	0.15	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	6	0.15	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	6	0.15	No Hit
TTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGGGAACTAAAGGGT	6	0.15	No Hit
CGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGG	6	0.15	No Hit
CCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTAC	6	0.15	No Hit
GGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCT	6	0.15	No Hit
AATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTT	6	0.15	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	6	0.15	No Hit
CTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGA	6	0.15	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	6	0.15	No Hit
CTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCC	6	0.15	No Hit
GCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCC	5	0.125	No Hit
CCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTC	5	0.125	No Hit
CCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATCT	5	0.125	No Hit
GTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG	5	0.125	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	5	0.125	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	5	0.125	No Hit
CTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAA	5	0.125	No Hit
CTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTCCAGATA	5	0.125	No Hit
CTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCT	5	0.125	No Hit
GCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATC	5	0.125	No Hit
GAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACT	5	0.125	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	5	0.125	No Hit
CAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCTATAACT	5	0.125	No Hit
ATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGATGTATTTGATGT	5	0.125	No Hit
TTCAGATTCACCTTCCACAGTTTTTAGTTACTGGTTGGTGGCCATCCACA	5	0.125	No Hit
CTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCACA	5	0.125	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	5	0.125	No Hit
ATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTCATC	5	0.125	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	5	0.125	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	5	0.125	No Hit
CCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCC	5	0.125	No Hit
GTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAATC	5	0.125	No Hit
TTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCTAT	5	0.125	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	5	0.125	No Hit
CCAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAG	5	0.125	No Hit
GTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAAC	5	0.125	No Hit
CACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATTT	5	0.125	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	5	0.125	No Hit
ACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTTAAAGCTA	5	0.125	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	5	0.125	No Hit
TGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATT	5	0.125	No Hit
CTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAAT	5	0.125	No Hit
CCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTCC	5	0.125	No Hit
TTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGG	5	0.125	No Hit
TAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATG	5	0.125	No Hit
CAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTTTTC	5	0.125	No Hit
GGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAG	5	0.125	No Hit
CTCCCTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCAT	5	0.125	No Hit
CTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACT	5	0.125	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	5	0.125	No Hit
CACCACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATAT	5	0.125	No Hit
TTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTT	5	0.125	No Hit
CATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACT	5	0.125	No Hit
CTGGGTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGG	5	0.125	No Hit
CCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTCG	5	0.125	No Hit
CGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGG	5	0.125	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	5	0.125	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	5	0.125	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	5	0.125	No Hit
CTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATT	5	0.125	No Hit
GCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATTTGATATC	5	0.125	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	5	0.125	No Hit
AGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATT	5	0.125	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	5	0.125	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	5	0.125	No Hit
GACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCC	5	0.125	No Hit
AATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTC	5	0.125	No Hit
CTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTA	5	0.125	No Hit
CCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATG	5	0.125	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTTTT	30	0.003469469	20.776316	70-74
>>END_MODULE
SRR25257915 read2 length is 53-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257915_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	53-151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.43	37.0	37.0	37.0	37.0	37.0
2	36.298	37.0	37.0	37.0	37.0	37.0
3	36.552	37.0	37.0	37.0	37.0	37.0
4	36.391	37.0	37.0	37.0	37.0	37.0
5	36.5545	37.0	37.0	37.0	37.0	37.0
6	36.157	37.0	37.0	37.0	37.0	37.0
7	36.51	37.0	37.0	37.0	37.0	37.0
8	36.631	37.0	37.0	37.0	37.0	37.0
9	36.5965	37.0	37.0	37.0	37.0	37.0
10-14	36.196	37.0	37.0	37.0	37.0	37.0
15-19	36.205400000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.25375	37.0	37.0	37.0	37.0	37.0
25-29	36.49395	37.0	37.0	37.0	37.0	37.0
30-34	36.34215	37.0	37.0	37.0	37.0	37.0
35-39	35.75445	37.0	37.0	37.0	37.0	37.0
40-44	36.39805	37.0	37.0	37.0	37.0	37.0
45-49	36.178200000000004	37.0	37.0	37.0	37.0	37.0
50-54	36.18238056528264	37.0	37.0	37.0	37.0	37.0
55-59	36.2067325462808	37.0	37.0	37.0	37.0	37.0
60-64	36.28369278187229	37.0	37.0	37.0	37.0	37.0
65-69	35.839731926821706	37.0	37.0	37.0	37.0	37.0
70-74	36.176399470582126	37.0	37.0	37.0	37.0	37.0
75-79	35.97592243772706	37.0	37.0	37.0	37.0	37.0
80-84	36.093344330530456	37.0	37.0	37.0	37.0	37.0
85-89	36.04067419642958	37.0	37.0	37.0	37.0	37.0
90-94	35.84115722621617	37.0	37.0	37.0	37.0	37.0
95-99	36.06419383684657	37.0	37.0	37.0	37.0	37.0
100-104	35.93535618571191	37.0	37.0	37.0	37.0	37.0
105-109	36.02478846390388	37.0	37.0	37.0	37.0	37.0
110-114	35.98703805373795	37.0	37.0	37.0	37.0	37.0
115-119	35.95995091447336	37.0	37.0	37.0	37.0	37.0
120-124	35.90940823564485	37.0	37.0	37.0	37.0	37.0
125-129	35.679672982047954	37.0	37.0	37.0	34.6	37.0
130-134	35.59762679149714	37.0	37.0	37.0	37.0	37.0
135-139	35.50446414562593	37.0	37.0	37.0	37.0	37.0
140-144	35.481888795812225	37.0	37.0	37.0	34.6	37.0
145-149	35.44444193547906	37.0	37.0	37.0	34.6	37.0
150-151	34.94096653669746	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	0.0
26	1.0
27	5.0
28	5.0
29	6.0
30	14.0
31	21.0
32	34.0
33	70.0
34	183.0
35	853.0
36	2573.0
37	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.55	14.325	20.525	34.599999999999994
2	24.219536757301107	24.823766364551865	31.64652567975831	19.31017119838872
3	23.5	26.5	26.974999999999998	23.025000000000002
4	25.95	34.875	20.125	19.05
5	23.95	37.45	22.025	16.575
6	22.06253151790217	39.00655572365103	21.55824508320726	17.372667675239537
7	20.775	18.15	39.475	21.6
8	21.325	24.65	28.425	25.6
9	22.05	25.124999999999996	29.549999999999997	23.275000000000002
10-14	24.266263237518913	28.59808371154816	24.029248613212303	23.106404437720627
15-19	24.01471625844169	30.042334442092532	24.886604172966432	21.056345126499345
20-24	21.962028503802188	31.51533464269527	25.885078309915897	20.637558543586646
25-29	23.50703308805126	28.99834810031536	25.554387545677532	21.94023126595585
30-34	23.82313129793954	30.37549506191407	24.730535920188498	21.07083771995789
35-39	23.3756306375172	29.144371400907097	25.81154767364827	21.66845028792743
40-44	23.167008658225313	30.173664981732646	26.054752014413694	20.604574345628347
45-49	22.337858220211164	30.749120160884868	26.244343891402718	20.668677727501255
50-54	23.082332847742002	30.496810167277843	25.624152308233285	20.79670467674687
55-59	23.12443507080446	29.140303304208093	25.816008838003413	21.919252786984032
60-64	23.326480469337614	30.47184475755904	25.673168530311386	20.52850624279196
65-69	23.24721377912867	30.070921985815602	25.861195542046605	20.820668693009118
70-74	23.168765743073045	30.26700251889169	25.989924433249374	20.574307304785894
75-79	23.70098414155321	31.145785528529906	24.960481362500637	20.192748967416247
80-84	23.077316163319537	31.105319638203284	26.09739894731463	19.719965251162552
85-89	23.3101443256152	31.227286886096977	25.516561104765863	19.94600768352196
90-94	22.72993868301786	31.959477472673953	25.225273260463876	20.085310583844308
95-99	23.176341730558597	31.281489594742606	26.089813800657176	19.45235487404162
100-104	23.247951406796172	31.419402899547304	25.57446564666781	19.75818004698871
105-109	22.04317238891227	32.42739462449642	25.344236666466237	20.18519632012507
110-114	22.244871958211238	31.55816027519429	27.18817683781373	19.008790928780737
115-119	23.31945676653245	31.77506312700471	26.445096567255856	18.460383539206987
120-124	21.546422678866055	32.86335683215839	25.371231438428076	20.218989050547474
125-129	22.746885939916957	31.116176829764715	26.15810469754946	19.978832532768866
130-134	23.346095628172023	31.653459175496394	25.70563618555783	19.29480901077375
135-139	21.250605913717887	32.66117304895783	26.951042171594764	19.13717886572952
140-144	22.472626767300945	33.3262464122462	25.651110874880406	18.550015945572447
145-149	22.6231866682652	32.29828557726891	25.452583623066776	19.625944131399113
150-151	22.07917605407145	31.638236240746703	26.231091084647566	20.05149662053428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	3.0
22	8.0
23	13.0
24	13.0
25	20.5
26	31.0
27	52.5
28	60.0
29	56.0
30	70.5
31	69.0
32	68.0
33	100.0
34	110.5
35	78.5
36	74.5
37	111.0
38	148.5
39	159.5
40	165.5
41	191.5
42	233.0
43	244.0
44	230.0
45	206.0
46	189.0
47	177.5
48	131.0
49	87.0
50	69.0
51	72.0
52	77.0
53	60.0
54	49.0
55	47.0
56	40.5
57	45.0
58	43.5
59	40.0
60	38.0
61	29.5
62	24.0
63	25.0
64	23.5
65	18.0
66	20.0
67	36.0
68	37.0
69	24.0
70	36.0
71	51.5
72	39.0
73	14.0
74	1.0
75	0.5
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.7000000000000001
3	0.0
4	0.0
5	0.0
6	0.8500000000000001
7	0.0
8	0.0
9	0.0
10-14	0.8500000000000001
15-19	0.79
20-24	0.715
25-29	0.11499999999999999
30-34	0.265
35-39	1.8849999999999998
40-44	0.095
45-49	0.5499999999999999
50-54	0.45504550455045506
55-59	0.3253416086891236
60-64	0.10018534288433602
65-69	0.9781790820165538
70-74	0.1458825896674883
75-79	0.7942128692836907
80-84	0.3107488537952114
85-89	0.3827058336781134
90-94	0.7199195384045313
95-99	0.2403846153846154
100-104	0.3141779961156175
105-109	0.11411411411411411
110-114	0.19074262461851477
115-119	0.19072270281315987
120-124	0.037484069270560014
125-129	0.2679441377070478
130-134	0.41674055683631844
135-139	0.39590575511780607
140-144	0.2544799066907009
145-149	0.4178605539637058
150-151	0.3847386983007374
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52-53	2.0
54-55	1.0
56-57	2.0
58-59	0.0
60-61	2.0
62-63	3.0
64-65	1.0
66-67	3.0
68-69	3.0
70-71	7.0
72-73	8.0
74-75	9.0
76-77	10.0
78-79	8.0
80-81	14.0
82-83	21.0
84-85	24.0
86-87	26.0
88-89	39.0
90-91	38.0
92-93	46.0
94-95	43.0
96-97	59.0
98-99	66.0
100-101	60.0
102-103	65.0
104-105	73.0
106-107	75.0
108-109	62.0
110-111	89.0
112-113	76.0
114-115	74.0
116-117	106.0
118-119	124.0
120-121	90.0
122-123	89.0
124-125	77.0
126-127	80.0
128-129	82.0
130-131	97.0
132-133	69.0
134-135	60.0
136-137	81.0
138-139	73.0
140-141	66.0
142-143	106.0
144-145	84.0
146-147	67.0
148-149	67.0
150-151	1573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24513618677042	45.775
2	16.57587548638132	21.3
3	6.031128404669261	11.625
4	2.9182879377431905	7.5
5	1.4785992217898831	4.75
6	0.5447470817120622	2.1
7	0.19455252918287938	0.8750000000000001
8	0.38910505836575876	2.0
9	0.38910505836575876	2.25
>10	0.23346303501945526	1.825
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	15	0.375	No Hit
CGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGG	14	0.35000000000000003	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	12	0.3	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	11	0.27499999999999997	No Hit
CTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGA	11	0.27499999999999997	No Hit
GAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATT	10	0.25	No Hit
TGTGATGCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTG	9	0.22499999999999998	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	9	0.22499999999999998	No Hit
CTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAG	9	0.22499999999999998	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	9	0.22499999999999998	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	9	0.22499999999999998	No Hit
CTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAA	9	0.22499999999999998	No Hit
TTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCT	9	0.22499999999999998	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	9	0.22499999999999998	No Hit
CAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAA	9	0.22499999999999998	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	9	0.22499999999999998	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	8	0.2	No Hit
CTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAA	8	0.2	No Hit
GAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCAA	8	0.2	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	8	0.2	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	8	0.2	No Hit
CTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTC	8	0.2	No Hit
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	8	0.2	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	8	0.2	No Hit
CCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAG	8	0.2	No Hit
CAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGT	8	0.2	No Hit
CAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGA	7	0.17500000000000002	No Hit
CGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGA	7	0.17500000000000002	No Hit
CGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCC	7	0.17500000000000002	No Hit
CTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCCGGAGCC	7	0.17500000000000002	No Hit
GGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCT	7	0.17500000000000002	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	6	0.15	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	6	0.15	No Hit
CAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAG	6	0.15	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	6	0.15	No Hit
GAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATA	6	0.15	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	6	0.15	No Hit
CGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCGCTGTG	6	0.15	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	6	0.15	No Hit
CCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTG	6	0.15	No Hit
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	6	0.15	No Hit
GGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAAT	6	0.15	No Hit
GGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACA	6	0.15	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	6	0.15	No Hit
TATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAG	6	0.15	No Hit
CTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAG	5	0.125	No Hit
GCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTG	5	0.125	No Hit
CAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAA	5	0.125	No Hit
CAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCC	5	0.125	No Hit
GCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATGTT	5	0.125	No Hit
CATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATG	5	0.125	No Hit
CAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGG	5	0.125	No Hit
TTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGG	5	0.125	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	5	0.125	No Hit
GGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCAC	5	0.125	No Hit
TGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAG	5	0.125	No Hit
TGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTG	5	0.125	No Hit
CAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCT	5	0.125	No Hit
CTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCC	5	0.125	No Hit
GGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATG	5	0.125	No Hit
GTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAA	5	0.125	No Hit
CCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTC	5	0.125	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	5	0.125	No Hit
TTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAATGT	5	0.125	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	5	0.125	No Hit
CCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACT	5	0.125	No Hit
CAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGA	5	0.125	No Hit
GTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATC	5	0.125	No Hit
CTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTTCCGGA	5	0.125	No Hit
ACGCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCG	5	0.125	No Hit
GGGAGGCAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGA	5	0.125	No Hit
TGCTGATGAACCAATGACAACATTTGTTGTATGTAATGAATGTGGAAATC	5	0.125	No Hit
TCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGC	5	0.125	No Hit
GTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATCTTGGCATAT	5	0.125	No Hit
AGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCG	5	0.125	No Hit
TTCTGTTTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGATGG	5	0.125	No Hit
CTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAAT	5	0.125	No Hit
TCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCT	5	0.125	No Hit
CAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTAGTCCAAC	5	0.125	No Hit
GCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGCC	5	0.125	No Hit
GACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAAT	5	0.125	No Hit
CTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGA	5	0.125	No Hit
CAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.025	0.0	0.0	0.0	0.0
120-121	0.025	0.0	0.0	0.0	0.0
122-123	0.025	0.0	0.0	0.0	0.0
124-125	0.025	0.0	0.0	0.0	0.0
126-127	0.025	0.0	0.0	0.0	0.0
128-129	0.025	0.0	0.0	0.0	0.0
130-131	0.025	0.0	0.0	0.0	0.0
132-133	0.025	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138-139	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
Rejected 388 READS because READLEN < 1
Read 4169584 spots for SRR25257915.sra
Written 4169584 spots for SRR25257915.sra
Read 4169583 spots for SRR25257915.sra
Written 4169583 spots for SRR25257915.sra
SRR ids: ['SRR25257915.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_lu1h07ym
SRR25257915.sra spots: 33356665
blocks: [[1, 4169583], [4169584, 8339166], [8339167, 12508749], [12508750, 16678332], [16678333, 20847915], [20847916, 25017498], [25017499, 29187081], [29187082, 33356665]]
SRR25257915 file size 10078076
SRR25257915 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25257915 SRR25257915_1.fastq SRR25257915_2.fastq
Input file:	SRR25257915_1.fastq
Paired file:	SRR25257915_2.fastq
trimmed:	SRR25257915-trimmed-pair1.fastq, SRR25257915-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu Jun 18 12:05:10 2026 >> started

Thu Jun 18 12:05:59 2026 >> done (49.092s)
33356287 read pairs processed; of these:
       2 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
33356285 (100.00%) read pairs available; of these:
  273394 ( 0.82%) trimmed read pairs available after processing
33082891 (99.18%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       6	  0.00%
 19	       9	  0.00%
 20	       8	  0.00%
 21	      10	  0.00%
 22	       6	  0.00%
 23	      14	  0.00%
 24	      10	  0.00%
 25	      16	  0.00%
 26	      12	  0.00%
 27	      24	  0.00%
 28	      22	  0.00%
 29	      38	  0.00%
 30	      67	  0.00%
 31	     166	  0.00%
 32	     243	  0.00%
 33	     251	  0.00%
 34	     323	  0.00%
 35	     325	  0.00%
 36	     365	  0.00%
 37	     441	  0.00%
 38	     531	  0.00%
 39	     589	  0.00%
 40	     707	  0.00%
 41	     908	  0.00%
 42	     996	  0.00%
 43	    1098	  0.00%
 44	    1304	  0.00%
 45	    1410	  0.00%
 46	    1685	  0.01%
 47	    1878	  0.01%
 48	    2177	  0.01%
 49	    2358	  0.01%
 50	    2641	  0.01%
 51	    3133	  0.01%
 52	    3568	  0.01%
 53	    4020	  0.01%
 54	    4557	  0.01%
 55	    4927	  0.01%
 56	    5513	  0.02%
 57	    5876	  0.02%
 58	    6685	  0.02%
 59	    7229	  0.02%
 60	    8296	  0.02%
 61	    9328	  0.03%
 62	   10597	  0.03%
 63	   12094	  0.04%
 64	   13979	  0.04%
 65	   14146	  0.04%
 66	   16298	  0.05%
 67	   17664	  0.05%
 68	   19349	  0.06%
 69	   21052	  0.06%
 70	   23478	  0.07%
 71	   26428	  0.08%
 72	   29905	  0.09%
 73	   33675	  0.10%
 74	   37903	  0.11%
 75	   42822	  0.13%
 76	   46380	  0.14%
 77	   50168	  0.15%
 78	   52765	  0.16%
 79	   59457	  0.18%
 80	   62882	  0.19%
 81	   67690	  0.20%
 82	   75905	  0.23%
 83	   83642	  0.25%
 84	   91677	  0.27%
 85	  100899	  0.30%
 86	  110349	  0.33%
 87	  117672	  0.35%
 88	  124044	  0.37%
 89	  134504	  0.40%
 90	  139252	  0.42%
 91	  150514	  0.45%
 92	  156086	  0.47%
 93	  172370	  0.52%
 94	  177147	  0.53%
 95	  195971	  0.59%
 96	  205010	  0.61%
 97	  215568	  0.65%
 98	  221054	  0.66%
 99	  228268	  0.68%
100	  240580	  0.72%
101	  243918	  0.73%
102	  250602	  0.75%
103	  261624	  0.78%
104	  275578	  0.83%
105	  282662	  0.85%
106	  294648	  0.88%
107	  305550	  0.92%
108	  311755	  0.93%
109	  320180	  0.96%
110	  312477	  0.94%
111	  313867	  0.94%
112	  321136	  0.96%
113	  320527	  0.96%
114	  324941	  0.97%
115	  333572	  1.00%
116	  337506	  1.01%
117	  352212	  1.06%
118	  346975	  1.04%
119	  348195	  1.04%
120	  346133	  1.04%
121	  345459	  1.04%
122	  344665	  1.03%
123	  338678	  1.02%
124	  343524	  1.03%
125	  339926	  1.02%
126	  337051	  1.01%
127	  343899	  1.03%
128	  351584	  1.05%
129	  336203	  1.01%
130	  345228	  1.03%
131	  335319	  1.01%
132	  327139	  0.98%
133	  333227	  1.00%
134	  314396	  0.94%
135	  320726	  0.96%
136	  316485	  0.95%
137	  324086	  0.97%
138	  307414	  0.92%
139	  314119	  0.94%
140	  314468	  0.94%
141	  305229	  0.92%
142	  308552	  0.93%
143	  297394	  0.89%
144	  297673	  0.89%
145	  299407	  0.90%
146	  381240	  1.14%
147	  450383	  1.35%
148	  356449	  1.07%
149	  269047	  0.81%
150	  364217	  1.09%
151	13506026	 40.49%
33356285 reads passed initial QC


criterion=sequence-density
sequence-density=0.59
sequence-density-rank=1
fanout-score=2.46
fanout-score-rank=29
prefix-density=0.63
prefix-fanout=2.3
sequence=TGTTGATCCAATAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=149.22
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=9.5
sequence=TTTTTAAATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAA


criterion=sequence-density
sequence-density=0.49
sequence-density-rank=1
fanout-score=4.05
fanout-score-rank=16
prefix-density=0.64
prefix-fanout=3.1
sequence=GGAGCCAGGAGACATATGAGACTCTGAAGCATGAGAAACCACCCCAGTAGCTTCAGAACAGACGTGCTTGGCTGCATTCTTTTCCCACTTCTAATTCTCTCCGAGCCCTCTTGGTCACCTCTGTGCTTTGAAGGTTGGCTGACCTTATTCACATAATGATGCTAGCTAGGCTCTACATCAGTGTACACTGGCAGGTCCCCATCTCCGTTAAAGACTTACTCACTGACATTTCTCTTCTTCCAGCCTCCTTTGCTTCATTTCTTTTTCCTTCCCTGATCCTCGACTCTCACTAAACAATGGAAAGGGATTATCCCCCAATAAAGCTGCCAGAGACCTG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=33
fanout-score=29.19
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=5.7
sequence=TGCTCCTGAGCTCGCGCGTTCCCTGCTTGCTGCCCTTCTGGCGCCCGCGCTGGTGGCACTACTGGTGTCGCCGGCCTCGGGTCGCGGAGGCCGGGACCACGGGGACTGGGACGTGGACAGGCGATTGCCTCCGCTGCCACCCCGCGAAGACGGGCCGCGCGTGGCCCGCTTCGTGACTCACGTCTCAGACTGGGGCTCGCTGGCCACTATCTCCACAATAAAGGAGGTGCGCGGCTGGCCCTTCGCGGACATCATCTCAATCAGTGACGGTCCTCCGGGAGAAGGCACGGGCGAGCCCTACATGTACCTGAGTCCACTGCAGCAAGCCGTGAGCGACCTGCAGGAAAATCCAGAGGCTACGCTGACTATGTCTTTAGCACAGACTGTCTACTGTAGGAATCATGGATTTGATCCCCAGAGTCCCCTGTGTGTTCATATAATGATGTCGGGAACTGTGACCAAGGTGAACAAGACAGAAGAGGACTATGCAAGGGATTCGCTGTTTGTTCGA
SRR25257915 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 18 12:06:21
                             Started mapping on |	Jun 18 12:06:21
                                    Finished on |	Jun 18 12:10:06
       Mapping speed, Million of reads per hour |	533.70

                          Number of input reads |	33356285
                      Average input read length |	264
                                    UNIQUE READS:
                   Uniquely mapped reads number |	31871141
                        Uniquely mapped reads % |	95.55%
                          Average mapped length |	264.11
                       Number of splices: Total |	33059922
            Number of splices: Annotated (sjdb) |	32843862
                       Number of splices: GT/AG |	32763822
                       Number of splices: GC/AG |	249453
                       Number of splices: AT/AC |	25518
               Number of splices: Non-canonical |	21129
                      Mismatch rate per base, % |	0.33%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.20
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.96
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1393379
             % of reads mapped to multiple loci |	4.18%
        Number of reads mapped to too many loci |	74187
             % of reads mapped to too many loci |	0.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.05%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	91826	91826	91826
N_multimapping	1393379	1393379	1393379
N_noFeature	1319334	30386950	1491286
N_ambiguous	2162712	263259	868276
UnstrandedReadsAssigned:28389095 PositiveStrandReadsAssigned:1220932 NegativeStrandReadsAssigned:29511579
Dataset is classified negative stranded
MeadianReadLen=140 20thPercentileLength=110 echo kmer=105
SRR25257915 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25257915-trimmed-pair1.fastq
                             SRR25257915-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 33,356,285 reads, 31,064,920 reads pseudoaligned
[quant] estimated average fragment length: 148.073
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,144 rounds

 109283 SRR25257915.ke.tsv
  52636 SRR25257915.se.tsv
 161919 total
==> SRR25257915.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25257915.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25257915 completed mapping pipeline successfully
