Starting /dee2/code/volunteer_pipeline.sh SRR25257911
    current disk space = 965301576
    free memory = 192266240 
SRR25257911 SRAfilesize
4deb1b5532edd03d050fd13897ba06cd  SRR25257911.sra
SRR25257911.sra file validated
SRR25257911 is paired end
SRR25257911 is conventional basespace
SRR25257911 read1 length is 47-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	47-151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.589	37.0	37.0	37.0	37.0	37.0
2	36.50225	37.0	37.0	37.0	37.0	37.0
3	36.57725	37.0	37.0	37.0	37.0	37.0
4	36.612	37.0	37.0	37.0	37.0	37.0
5	36.5565	37.0	37.0	37.0	37.0	37.0
6	36.5705	37.0	37.0	37.0	37.0	37.0
7	36.5825	37.0	37.0	37.0	37.0	37.0
8	36.631	37.0	37.0	37.0	37.0	37.0
9	36.5865	37.0	37.0	37.0	37.0	37.0
10-14	36.617599999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.6007	37.0	37.0	37.0	37.0	37.0
20-24	36.5476	37.0	37.0	37.0	37.0	37.0
25-29	36.4659	37.0	37.0	37.0	37.0	37.0
30-34	36.347950000000004	37.0	37.0	37.0	37.0	37.0
35-39	36.35275	37.0	37.0	37.0	37.0	37.0
40-44	36.47539999999999	37.0	37.0	37.0	37.0	37.0
45-49	36.33771487871968	37.0	37.0	37.0	37.0	37.0
50-54	36.25985014276034	37.0	37.0	37.0	37.0	37.0
55-59	36.20366384848491	37.0	37.0	37.0	37.0	37.0
60-64	36.33058255323785	37.0	37.0	37.0	37.0	37.0
65-69	36.32051291240556	37.0	37.0	37.0	37.0	37.0
70-74	36.32073683533472	37.0	37.0	37.0	37.0	37.0
75-79	36.22629162063748	37.0	37.0	37.0	37.0	37.0
80-84	36.265442527147634	37.0	37.0	37.0	37.0	37.0
85-89	36.170035038476314	37.0	37.0	37.0	37.0	37.0
90-94	36.193001473615276	37.0	37.0	37.0	37.0	37.0
95-99	36.14066547265159	37.0	37.0	37.0	37.0	37.0
100-104	35.912625817564376	37.0	37.0	37.0	37.0	37.0
105-109	36.10916790636558	37.0	37.0	37.0	37.0	37.0
110-114	36.08891711165873	37.0	37.0	37.0	37.0	37.0
115-119	35.93426572419652	37.0	37.0	37.0	37.0	37.0
120-124	36.024776150813565	37.0	37.0	37.0	37.0	37.0
125-129	35.97816998844367	37.0	37.0	37.0	37.0	37.0
130-134	35.83404058486848	37.0	37.0	37.0	37.0	37.0
135-139	35.69264702285932	37.0	37.0	37.0	37.0	37.0
140-144	35.85184368031314	37.0	37.0	37.0	37.0	37.0
145-149	35.73640566163097	37.0	37.0	37.0	37.0	37.0
150-151	35.1746838581701	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	1.0
27	6.0
28	8.0
29	13.0
30	23.0
31	20.0
32	33.0
33	59.0
34	94.0
35	429.0
36	2969.0
37	343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.6	11.35	21.675	39.375
2	18.35458864716179	18.404601150287572	41.21030257564391	22.030507626906726
3	22.18054513628407	21.355338834708675	30.682670667666915	25.78144536134033
4	20.200000000000003	31.15	28.050000000000004	20.599999999999998
5	22.075	33.125	31.874999999999996	12.925
6	18.975	30.275000000000002	33.925	16.825000000000003
7	12.1	18.875	52.300000000000004	16.725
8	17.075000000000003	20.200000000000003	37.5	25.224999999999998
9	17.775	19.825	41.5	20.9
10-14	19.375	25.874999999999996	31.465	23.285
15-19	19.6	26.340000000000003	32.405	21.654999999999998
20-24	19.645000000000003	25.019999999999996	32.235	23.1
25-29	18.231462925851705	24.71943887775551	32.359719438877754	24.68937875751503
30-34	20.285126248682296	27.2325686461523	30.43019928718438	22.052105817981023
35-39	19.811462668605525	24.730481873339016	32.58286115428973	22.875194303765735
40-44	20.200000000000003	23.955000000000002	31.269999999999996	24.575
45-49	19.07604333868379	25.32604333868379	31.93218298555377	23.665730337078653
50-54	20.57656571744262	25.26744010848275	30.94771734217267	23.208276831901962
55-59	21.848231381638616	24.065302831804896	29.99093016224932	24.095535624307164
60-64	23.02060873489445	26.335054906483478	29.10795767938625	21.53637867923582
65-69	21.968365553602812	25.63896560381622	29.294501631935727	23.098167210645244
70-74	21.176173649671885	25.835436648157494	30.08581524482585	22.902574457344777
75-79	22.255086949869956	24.932428986689786	30.86337906063542	21.949105002804835
80-84	20.63418166770122	25.537968135733497	30.829712393958204	22.998137802607076
85-89	21.78306878306878	25.264550264550266	30.02645502645503	22.925925925925924
90-94	23.39569284315858	25.71785947356972	29.307156841418312	21.579290841853382
95-99	20.655887041676156	25.11386927806878	30.75609200637668	23.47415167387839
100-104	21.384485053854533	23.98039452983178	30.122231634999398	24.51288878131429
105-109	22.43540547454592	24.87848554617549	28.96520849322077	23.720900486057815
110-114	21.41874225952938	24.80390807761112	28.34732351726985	25.43002614558965
115-119	24.107809466925488	25.94445274003285	28.714349708824848	21.233388084216813
120-124	21.965979718678444	24.517500817795224	31.526005888125614	21.99051357540072
125-129	21.492086075048906	27.574248621732174	28.881380046238665	22.05228525698026
130-134	20.744103424454753	25.96466989045692	31.135892628046975	22.15533405704135
135-139	20.741713160485723	26.10217700470408	30.67498085548627	22.481128979323923
140-144	21.846078908913785	25.669751583049194	28.726254262055527	23.75791524598149
145-149	21.548821548821547	26.79573512906846	28.52132435465769	23.1341189674523
150-151	22.328244274809162	24.770992366412216	30.38167938931298	22.519083969465647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5
22	3.5
23	4.0
24	6.5
25	11.5
26	29.5
27	42.5
28	48.0
29	68.5
30	71.5
31	49.0
32	38.5
33	67.0
34	97.5
35	90.0
36	107.5
37	150.0
38	146.5
39	146.5
40	175.0
41	213.0
42	211.0
43	214.0
44	248.0
45	218.0
46	203.0
47	196.0
48	169.5
49	143.0
50	114.0
51	96.0
52	87.0
53	87.0
54	79.5
55	57.5
56	39.0
57	37.5
58	38.5
59	47.0
60	48.0
61	26.5
62	17.0
63	22.0
64	15.5
65	9.0
66	7.0
67	17.0
68	19.5
69	12.0
70	11.0
71	11.5
72	8.0
73	2.5
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.2
30-34	0.395
35-39	0.28500000000000003
40-44	0.0
45-49	0.31003100310031007
50-54	0.3553197878090281
55-59	0.5860842558733658
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.14767287911192584
80-84	0.0
85-89	0.1690259877456159
90-94	0.14119691539046378
95-99	0.36305877013841614
100-104	0.7983672489345098
105-109	0.14688038827511335
110-114	0.2196896883152547
115-119	0.4237603152181994
120-124	0.06538084341288003
125-129	0.24835905623558632
130-134	0.6958055664445316
135-139	0.878334417696812
140-144	0.33980582524271846
145-149	0.9312022237665045
150-151	0.7575757575757576
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	1.0
50-54	5.0
55-59	5.0
60-64	3.0
65-69	10.0
70-74	32.0
75-79	57.0
80-84	72.0
85-89	87.0
90-94	127.0
95-99	201.0
100-104	183.0
105-109	221.0
110-114	219.0
115-119	243.0
120-124	201.0
125-129	201.0
130-134	206.0
135-139	203.0
140-144	192.0
145-149	199.0
150-152	1332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.074999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.61785912368275	28.225
2	15.52967276760954	14.000000000000002
3	6.932889628397115	9.375
4	4.437049362174155	8.0
5	2.995008319467554	6.75
6	1.8302828618968388	4.95
7	1.3311148086522462	4.2
8	0.7210205213533	2.6
9	0.7210205213533	2.9250000000000003
>10	2.8840820854132	18.975
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	40	1.0	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	24	0.6	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	21	0.525	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	20	0.5	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	20	0.5	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	20	0.5	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	19	0.475	No Hit
CTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAA	19	0.475	No Hit
CAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTG	18	0.44999999999999996	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	17	0.42500000000000004	No Hit
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	17	0.42500000000000004	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	17	0.42500000000000004	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	17	0.42500000000000004	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	16	0.4	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	16	0.4	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	16	0.4	No Hit
CTCATCCTTTCTGCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTG	16	0.4	No Hit
CACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGC	15	0.375	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	15	0.375	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	15	0.375	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	15	0.375	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	15	0.375	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	14	0.35000000000000003	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	14	0.35000000000000003	No Hit
TTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGC	13	0.325	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	13	0.325	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	13	0.325	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	13	0.325	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	13	0.325	No Hit
CCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATG	13	0.325	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	12	0.3	No Hit
GTGGACTGTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAG	12	0.3	No Hit
CTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCT	12	0.3	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	12	0.3	No Hit
GTTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTT	12	0.3	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	12	0.3	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	12	0.3	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	12	0.3	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	11	0.27499999999999997	No Hit
GTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGA	11	0.27499999999999997	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	11	0.27499999999999997	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	11	0.27499999999999997	No Hit
GTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCT	11	0.27499999999999997	No Hit
GTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGG	11	0.27499999999999997	No Hit
GAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAGGT	11	0.27499999999999997	No Hit
TTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCAA	11	0.27499999999999997	No Hit
CTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCC	11	0.27499999999999997	No Hit
GCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCC	10	0.25	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	10	0.25	No Hit
TCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATTCT	10	0.25	No Hit
GAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTT	10	0.25	No Hit
CTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAAT	10	0.25	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	9	0.22499999999999998	No Hit
TTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAG	9	0.22499999999999998	No Hit
CAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAA	9	0.22499999999999998	No Hit
GTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAATC	9	0.22499999999999998	No Hit
CCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGA	9	0.22499999999999998	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	9	0.22499999999999998	No Hit
GAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGC	9	0.22499999999999998	No Hit
CTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAAT	9	0.22499999999999998	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	9	0.22499999999999998	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	9	0.22499999999999998	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	9	0.22499999999999998	No Hit
TAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCA	9	0.22499999999999998	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	9	0.22499999999999998	No Hit
CTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCA	8	0.2	No Hit
CGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTG	8	0.2	No Hit
CCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTTTT	8	0.2	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	8	0.2	No Hit
GCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTG	8	0.2	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	8	0.2	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	8	0.2	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	8	0.2	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	8	0.2	No Hit
GTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGC	8	0.2	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	8	0.2	No Hit
ACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATG	8	0.2	No Hit
ATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGC	8	0.2	No Hit
CTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAA	7	0.17500000000000002	No Hit
GGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTC	7	0.17500000000000002	No Hit
GCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATA	7	0.17500000000000002	No Hit
ATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCA	7	0.17500000000000002	No Hit
GCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCC	7	0.17500000000000002	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	7	0.17500000000000002	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	7	0.17500000000000002	No Hit
CACCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCATC	7	0.17500000000000002	No Hit
TTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAAT	7	0.17500000000000002	No Hit
CCCACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGAT	7	0.17500000000000002	No Hit
TCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCC	7	0.17500000000000002	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	7	0.17500000000000002	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	7	0.17500000000000002	No Hit
GCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATTTGATATC	7	0.17500000000000002	No Hit
GCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTAT	7	0.17500000000000002	No Hit
TGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCC	7	0.17500000000000002	No Hit
CTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGC	7	0.17500000000000002	No Hit
CAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACAT	7	0.17500000000000002	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	7	0.17500000000000002	No Hit
AATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTT	7	0.17500000000000002	No Hit
TTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCC	7	0.17500000000000002	No Hit
CTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCC	7	0.17500000000000002	No Hit
GTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG	6	0.15	No Hit
TGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCAC	6	0.15	No Hit
CTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCA	6	0.15	No Hit
CGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACTGGC	6	0.15	No Hit
TTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATTT	6	0.15	No Hit
TTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCTC	6	0.15	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	6	0.15	No Hit
TTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCC	6	0.15	No Hit
AAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGCTCC	6	0.15	No Hit
GTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCT	6	0.15	No Hit
GGTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCA	6	0.15	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	6	0.15	No Hit
GGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATG	6	0.15	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	6	0.15	No Hit
TGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATT	6	0.15	No Hit
TGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCACATTTT	6	0.15	No Hit
CGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCT	6	0.15	No Hit
TGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATG	6	0.15	No Hit
GCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAATCCGTGAAGGAA	6	0.15	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	6	0.15	No Hit
CTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTCATCAGCT	6	0.15	No Hit
AATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCAT	6	0.15	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	6	0.15	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	6	0.15	No Hit
AGAGAGGTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGA	6	0.15	No Hit
CTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTC	6	0.15	No Hit
TTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGGGAACTAAAGGGT	6	0.15	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	6	0.15	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	6	0.15	No Hit
TCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATATCAAACC	6	0.15	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	6	0.15	No Hit
AATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTC	6	0.15	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	6	0.15	No Hit
CTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATTTTTTCC	5	0.125	No Hit
TTCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATAT	5	0.125	No Hit
GCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATTT	5	0.125	No Hit
TATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAG	5	0.125	No Hit
CGAGCATTTGTCTCATCCTTTCTGCTGCTTACATTGCTGCTGGAACTACT	5	0.125	No Hit
GCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAAT	5	0.125	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	5	0.125	No Hit
CTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATTCTT	5	0.125	No Hit
CAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGA	5	0.125	No Hit
CCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCC	5	0.125	No Hit
CGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCACCACCACG	5	0.125	No Hit
TGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTG	5	0.125	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	5	0.125	No Hit
CTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGA	5	0.125	No Hit
CAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTG	5	0.125	No Hit
TTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCTG	5	0.125	No Hit
CAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGAAACATATGTATCT	5	0.125	No Hit
CTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAG	5	0.125	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	5	0.125	No Hit
GTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGG	5	0.125	No Hit
AATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTG	5	0.125	No Hit
CTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCG	5	0.125	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	5	0.125	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	5	0.125	No Hit
TATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGGT	5	0.125	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	5	0.125	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	5	0.125	No Hit
CCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCATCAG	5	0.125	No Hit
CTACTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAAT	5	0.125	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	5	0.125	No Hit
GTCCATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAG	5	0.125	No Hit
CCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAGT	5	0.125	No Hit
CTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTT	5	0.125	No Hit
GAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAA	5	0.125	No Hit
GTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCC	5	0.125	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	5	0.125	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	5	0.125	No Hit
ATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGA	5	0.125	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	5	0.125	No Hit
TTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGC	5	0.125	No Hit
CCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGA	5	0.125	No Hit
CAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCT	5	0.125	No Hit
GCATCTTTAAGATTTGATATCCTACTTCGTACTCTGTTTTTGTATTTCAT	5	0.125	No Hit
AGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATT	5	0.125	No Hit
CTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTT	5	0.125	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	5	0.125	No Hit
TTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCATA	5	0.125	No Hit
ATTGGTTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTT	5	0.125	No Hit
CTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCACATTT	5	0.125	No Hit
CTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGA	5	0.125	No Hit
GGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTG	5	0.125	No Hit
CATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTG	5	0.125	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	5	0.125	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25257911 read2 length is 39-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257911_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4835	37.0	37.0	37.0	37.0	37.0
2	36.21625	37.0	37.0	37.0	37.0	37.0
3	36.486	37.0	37.0	37.0	37.0	37.0
4	36.4615	37.0	37.0	37.0	37.0	37.0
5	36.352	37.0	37.0	37.0	37.0	37.0
6	36.0805	37.0	37.0	37.0	37.0	37.0
7	36.6295	37.0	37.0	37.0	37.0	37.0
8	36.492	37.0	37.0	37.0	37.0	37.0
9	36.5995	37.0	37.0	37.0	37.0	37.0
10-14	36.26115	37.0	37.0	37.0	37.0	37.0
15-19	36.186749999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.2362	37.0	37.0	37.0	37.0	37.0
25-29	36.4514	37.0	37.0	37.0	37.0	37.0
30-34	36.3552	37.0	37.0	37.0	37.0	37.0
35-39	35.81415	37.0	37.0	37.0	37.0	37.0
40-44	36.39629907476869	37.0	37.0	37.0	37.0	37.0
45-49	36.1825456364091	37.0	37.0	37.0	37.0	37.0
50-54	36.19614778001492	37.0	37.0	37.0	37.0	37.0
55-59	36.23410923763612	37.0	37.0	37.0	37.0	37.0
60-64	36.269581030838914	37.0	37.0	37.0	37.0	37.0
65-69	35.926228085254905	37.0	37.0	37.0	37.0	37.0
70-74	36.21254090252911	37.0	37.0	37.0	37.0	37.0
75-79	35.961762110829945	37.0	37.0	37.0	37.0	37.0
80-84	36.069751936968565	37.0	37.0	37.0	37.0	37.0
85-89	35.96864584997555	37.0	37.0	37.0	37.0	37.0
90-94	35.800430174990154	37.0	37.0	37.0	37.0	37.0
95-99	36.077088481172595	37.0	37.0	37.0	37.0	37.0
100-104	35.91137573224786	37.0	37.0	37.0	37.0	37.0
105-109	36.057237622342946	37.0	37.0	37.0	37.0	37.0
110-114	35.99170636462088	37.0	37.0	37.0	37.0	37.0
115-119	35.95543737034258	37.0	37.0	37.0	37.0	37.0
120-124	35.934779929073024	37.0	37.0	37.0	37.0	37.0
125-129	35.827962464604795	37.0	37.0	37.0	37.0	37.0
130-134	35.726124645066406	37.0	37.0	37.0	37.0	37.0
135-139	35.625504736291774	37.0	37.0	37.0	37.0	37.0
140-144	35.65969369999921	37.0	37.0	37.0	37.0	37.0
145-149	35.53766274413734	37.0	37.0	37.0	34.6	37.0
150-151	34.92979656821038	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	1.0
27	4.0
28	8.0
29	9.0
30	10.0
31	21.0
32	31.0
33	44.0
34	180.0
35	875.0
36	2540.0
37	275.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.9	14.499999999999998	19.6	33.0
2	24.19802980550644	24.12225309421571	31.67466531952513	20.005051780752716
3	23.200000000000003	26.6	26.85	23.35
4	27.800000000000004	32.85	20.025000000000002	19.325
5	26.325	35.65	21.2	16.825000000000003
6	21.263210870659286	39.17966784096628	21.7916456970307	17.765475591343733
7	21.45	20.1	38.1	20.349999999999998
8	22.025	23.150000000000002	27.700000000000003	27.125
9	23.625	25.95	27.400000000000002	23.025000000000002
10-14	24.925621501689275	28.14784932681156	22.974131410418032	23.952397761081134
15-19	23.80976422476902	30.256979855606602	24.30958751956379	21.623668400060584
20-24	22.981147293073896	31.580804516584333	24.64462143361226	20.79342675672951
25-29	23.212139423076923	30.128205128205128	24.554286858974358	22.10536858974359
30-34	23.471232051410784	29.94276533788533	25.102921980118488	21.4830806305854
35-39	24.074922380007127	29.673741538148317	24.980913116506336	21.27042296533822
40-44	22.876177118813864	30.189340813464238	24.98497295131236	21.949509116409537
45-49	22.844784226938938	30.238406598933707	25.721758374409013	21.195050799718338
50-54	22.93458037914215	31.357167999195457	24.257052345753507	21.451199275908888
55-59	22.599125584200213	30.202522739836173	24.60425146992311	22.594100206040505
60-64	23.986537398904908	30.31094589842769	24.25277540563621	21.449741297031196
65-69	23.47367887723565	30.698687743831382	24.289405684754524	21.538227694178445
70-74	22.78775613458133	30.10877814318239	26.471034657222365	20.632431065013915
75-79	23.44367462150372	30.377213240954582	25.229663843982554	20.949448293559147
80-84	22.80191228434837	31.568281022656414	25.92496362502598	19.70484306796924
85-89	22.527821939586644	31.679915209326975	25.26762056173821	20.524642289348172
90-94	22.92749658002736	31.463748290013683	25.088919288645688	20.51983584131327
95-99	23.049322246269508	31.774689600182253	25.213577856247866	19.962410297300377
100-104	23.375445807894575	32.424590461222266	24.42120534364988	19.77875838723327
105-109	22.67505294910468	31.814389320326036	24.15762788011039	21.352929850458892
110-114	22.83290807530515	32.09433832149507	26.23956968484932	18.833183918350457
115-119	24.316267978239807	31.61189358372457	26.000447127207693	18.07139131082793
120-124	22.676367203466036	33.891931660263225	23.19136761219652	20.240333524074224
125-129	23.914976876556384	31.696905016008536	24.235147634293845	20.15297047314123
130-134	22.916256157635466	32.285714285714285	25.182266009852217	19.61576354679803
135-139	22.196821249727847	31.776616590463746	25.78924450250381	20.237317657304594
140-144	22.52076209086468	33.47581827063996	25.622862725940397	18.380556912554958
145-149	22.510212706014933	33.30046485420482	24.510494435836033	19.67882800394422
150-151	22.891104294478527	33.39723926380368	26.265337423312886	17.446319018404907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	2.0
21	2.5
22	4.5
23	6.0
24	10.5
25	19.5
26	26.5
27	34.0
28	50.0
29	62.5
30	52.5
31	47.5
32	62.0
33	98.5
34	101.5
35	64.0
36	85.0
37	108.5
38	114.0
39	123.0
40	138.0
41	178.5
42	228.5
43	258.5
44	276.0
45	257.5
46	205.0
47	181.5
48	158.5
49	123.5
50	95.0
51	81.5
52	86.0
53	82.5
54	61.0
55	43.5
56	38.0
57	45.0
58	44.0
59	38.5
60	33.5
61	27.5
62	24.5
63	18.5
64	21.5
65	26.0
66	27.5
67	31.0
68	30.0
69	23.0
70	22.0
71	44.5
72	44.5
73	16.0
74	3.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.0250000000000001
3	0.0
4	0.0
5	0.0
6	0.65
7	0.0
8	0.0
9	0.0
10-14	0.845
15-19	0.9650000000000001
20-24	0.8099999999999999
25-29	0.16
30-34	0.41000000000000003
35-39	1.765
40-44	0.15503875968992248
45-49	0.5651412853213303
50-54	0.47542788509658696
55-59	0.3205930972298753
60-64	0.16549648946840523
65-69	0.8440090429540317
70-74	0.14650904314438717
75-79	0.7285510495210923
80-84	0.3779054718641611
85-89	0.3538047209167239
90-94	0.7386888273314866
95-99	0.2726343292059525
100-104	0.445327074682554
105-109	0.09617850730956655
110-114	0.14460818069136483
115-119	0.14882059677059303
120-124	0.08983992159425024
125-129	0.2660989888238425
130-134	0.36320801020909
135-139	0.43355733795794493
140-144	0.08541793776693106
145-149	0.3369366839814685
150-151	0.45801526717557256
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	1.0
40-44	0.0
45-49	0.0
50-54	5.0
55-59	5.0
60-64	4.0
65-69	13.0
70-74	30.0
75-79	59.0
80-84	67.0
85-89	88.0
90-94	130.0
95-99	203.0
100-104	191.0
105-109	220.0
110-114	210.0
115-119	237.0
120-124	197.0
125-129	212.0
130-134	202.0
135-139	207.0
140-144	208.0
145-149	189.0
150-152	1322.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.775000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.343786750294	46.775
2	14.62171697373579	18.65
3	5.056840454723638	9.675
4	2.7440219521756175	7.000000000000001
5	1.803214425715406	5.75
6	1.0192081536652293	3.9
7	0.3920031360250882	1.7500000000000002
8	0.4704037632301058	2.4
9	0.07840062720501764	0.44999999999999996
>10	0.4704037632301058	3.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	19	0.475	No Hit
CTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGA	15	0.375	No Hit
TGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGA	13	0.325	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	13	0.325	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	12	0.3	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	11	0.27499999999999997	No Hit
CTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAA	11	0.27499999999999997	No Hit
TTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCT	11	0.27499999999999997	No Hit
CAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAA	11	0.27499999999999997	No Hit
GGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATG	10	0.25	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	10	0.25	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	10	0.25	No Hit
TGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAG	9	0.22499999999999998	No Hit
CGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGA	9	0.22499999999999998	No Hit
GTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATC	8	0.2	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	8	0.2	No Hit
CTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAA	8	0.2	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	8	0.2	No Hit
TCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGC	8	0.2	No Hit
CACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATG	8	0.2	No Hit
CGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAAT	8	0.2	No Hit
GAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATT	8	0.2	No Hit
CCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACG	8	0.2	No Hit
ATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGC	8	0.2	No Hit
GGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAAT	8	0.2	No Hit
CCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGA	8	0.2	No Hit
GTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTC	7	0.17500000000000002	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	7	0.17500000000000002	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	7	0.17500000000000002	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	7	0.17500000000000002	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	7	0.17500000000000002	No Hit
AGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCG	7	0.17500000000000002	No Hit
GGCCGGGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGC	7	0.17500000000000002	No Hit
CTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAG	7	0.17500000000000002	No Hit
TGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTAT	7	0.17500000000000002	No Hit
CAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTA	7	0.17500000000000002	No Hit
TTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCA	6	0.15	No Hit
CAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAA	6	0.15	No Hit
GGAGGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGA	6	0.15	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	6	0.15	No Hit
CATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATG	6	0.15	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	6	0.15	No Hit
GAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGC	6	0.15	No Hit
TTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACT	6	0.15	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	6	0.15	No Hit
GCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTT	6	0.15	No Hit
GTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGA	6	0.15	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	6	0.15	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	6	0.15	No Hit
CCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACT	6	0.15	No Hit
CAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCA	6	0.15	No Hit
TTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTC	6	0.15	No Hit
GGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACA	6	0.15	No Hit
CTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGG	6	0.15	No Hit
CAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCA	6	0.15	No Hit
GGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAA	6	0.15	No Hit
CTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAA	6	0.15	No Hit
GCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCA	6	0.15	No Hit
AAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGAC	6	0.15	No Hit
TTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATC	6	0.15	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	6	0.15	No Hit
GCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCCA	6	0.15	No Hit
GCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCA	5	0.125	No Hit
GTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTG	5	0.125	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	5	0.125	No Hit
TTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCT	5	0.125	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	5	0.125	No Hit
ATGAGACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCA	5	0.125	No Hit
TCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCG	5	0.125	No Hit
AAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTC	5	0.125	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	5	0.125	No Hit
ATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAA	5	0.125	No Hit
CGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTG	5	0.125	No Hit
GCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAAT	5	0.125	No Hit
GTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCT	5	0.125	No Hit
CTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTTTT	5	0.125	No Hit
GCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGC	5	0.125	No Hit
GACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCA	5	0.125	No Hit
CGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGG	5	0.125	No Hit
CTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCC	5	0.125	No Hit
GTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCT	5	0.125	No Hit
GTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGA	5	0.125	No Hit
CAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGACTGACCATAGAA	5	0.125	No Hit
ATCAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGT	5	0.125	No Hit
CTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCG	5	0.125	No Hit
GGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGG	5	0.125	No Hit
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	5	0.125	No Hit
GCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGG	5	0.125	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	5	0.125	No Hit
TTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTC	5	0.125	No Hit
CTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGG	5	0.125	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	5	0.125	No Hit
GTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGC	5	0.125	No Hit
CACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACA	5	0.125	No Hit
GAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTG	5	0.125	No Hit
GGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACA	5	0.125	No Hit
CCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATC	5	0.125	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	5	0.125	No Hit
CTGGCTTCAAAATATAATTTCTTAAATATCTTATAGCCGATTTTAAGTTT	5	0.125	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	5	0.125	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	5	0.125	No Hit
ATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCC	5	0.125	No Hit
ATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGG	5	0.125	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	5	0.125	No Hit
GAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAG	5	0.125	No Hit
CCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATT	5	0.125	No Hit
TTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAA	5	0.125	No Hit
GGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Read 3817263 spots for SRR25257911.sra
Written 3817263 spots for SRR25257911.sra
Rejected 630 READS because READLEN < 1
Read 3817264 spots for SRR25257911.sra
Written 3817264 spots for SRR25257911.sra
SRR ids: ['SRR25257911.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9g8fh0to
SRR25257911.sra spots: 30538105
blocks: [[1, 3817263], [3817264, 7634526], [7634527, 11451789], [11451790, 15269052], [15269053, 19086315], [19086316, 22903578], [22903579, 26720841], [26720842, 30538105]]
SRR25257911 file size 9029452
SRR25257911 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25257911 SRR25257911_1.fastq SRR25257911_2.fastq
Input file:	SRR25257911_1.fastq
Paired file:	SRR25257911_2.fastq
trimmed:	SRR25257911-trimmed-pair1.fastq, SRR25257911-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu Jun 18 13:18:35 2026 >> started

Thu Jun 18 13:23:39 2026 >> done (304.228s)
30537482 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
30537482 (100.00%) read pairs available; of these:
  249157 ( 0.82%) trimmed read pairs available after processing
30288325 (99.18%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       5	  0.00%
 19	       4	  0.00%
 20	      11	  0.00%
 21	      12	  0.00%
 22	       9	  0.00%
 23	      10	  0.00%
 24	      15	  0.00%
 25	      19	  0.00%
 26	      22	  0.00%
 27	      34	  0.00%
 28	      64	  0.00%
 29	      84	  0.00%
 30	     120	  0.00%
 31	     244	  0.00%
 32	     302	  0.00%
 33	     350	  0.00%
 34	     522	  0.00%
 35	     509	  0.00%
 36	     564	  0.00%
 37	     768	  0.00%
 38	     844	  0.00%
 39	     973	  0.00%
 40	    1136	  0.00%
 41	    1401	  0.00%
 42	    1660	  0.01%
 43	    1924	  0.01%
 44	    2081	  0.01%
 45	    2413	  0.01%
 46	    2603	  0.01%
 47	    2904	  0.01%
 48	    3177	  0.01%
 49	    3659	  0.01%
 50	    3935	  0.01%
 51	    4650	  0.02%
 52	    5543	  0.02%
 53	    5847	  0.02%
 54	    6737	  0.02%
 55	    7133	  0.02%
 56	    7776	  0.03%
 57	    8670	  0.03%
 58	    9243	  0.03%
 59	   10519	  0.03%
 60	   11732	  0.04%
 61	   13151	  0.04%
 62	   14302	  0.05%
 63	   16547	  0.05%
 64	   19360	  0.06%
 65	   19581	  0.06%
 66	   21881	  0.07%
 67	   23724	  0.08%
 68	   26046	  0.09%
 69	   28901	  0.09%
 70	   31620	  0.10%
 71	   35114	  0.11%
 72	   39864	  0.13%
 73	   43906	  0.14%
 74	   48792	  0.16%
 75	   54765	  0.18%
 76	   59325	  0.19%
 77	   64263	  0.21%
 78	   66821	  0.22%
 79	   75621	  0.25%
 80	   79574	  0.26%
 81	   86564	  0.28%
 82	   96051	  0.31%
 83	  104414	  0.34%
 84	  112078	  0.37%
 85	  122807	  0.40%
 86	  133020	  0.44%
 87	  141163	  0.46%
 88	  150077	  0.49%
 89	  161199	  0.53%
 90	  165911	  0.54%
 91	  178143	  0.58%
 92	  181933	  0.60%
 93	  198828	  0.65%
 94	  202851	  0.66%
 95	  222319	  0.73%
 96	  230848	  0.76%
 97	  242359	  0.79%
 98	  246435	  0.81%
 99	  251342	  0.82%
100	  269053	  0.88%
101	  270353	  0.89%
102	  273870	  0.90%
103	  284961	  0.93%
104	  299209	  0.98%
105	  300331	  0.98%
106	  312933	  1.02%
107	  321997	  1.05%
108	  324402	  1.06%
109	  333073	  1.09%
110	  322045	  1.05%
111	  325620	  1.07%
112	  329444	  1.08%
113	  327198	  1.07%
114	  330115	  1.08%
115	  335935	  1.10%
116	  335941	  1.10%
117	  347483	  1.14%
118	  342330	  1.12%
119	  343188	  1.12%
120	  335845	  1.10%
121	  335471	  1.10%
122	  335166	  1.10%
123	  329400	  1.08%
124	  332324	  1.09%
125	  326364	  1.07%
126	  321151	  1.05%
127	  325249	  1.07%
128	  331020	  1.08%
129	  314988	  1.03%
130	  321077	  1.05%
131	  306159	  1.00%
132	  300349	  0.98%
133	  306316	  1.00%
134	  291117	  0.95%
135	  296429	  0.97%
136	  288029	  0.94%
137	  295473	  0.97%
138	  278479	  0.91%
139	  282915	  0.93%
140	  283113	  0.93%
141	  274565	  0.90%
142	  280047	  0.92%
143	  265060	  0.87%
144	  265794	  0.87%
145	  263994	  0.86%
146	  328936	  1.08%
147	  382981	  1.25%
148	  305015	  1.00%
149	  237822	  0.78%
150	  308804	  1.01%
151	10636821	 34.83%
30537482 reads passed initial QC


criterion=sequence-density
sequence-density=0.88
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=40
prefix-density=0.89
prefix-fanout=2.0
sequence=AAAAGCAGGAGGAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=97.16
fanout-score-rank=1
prefix-density=0.10
prefix-fanout=6.0
sequence=TTTTTAAATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAA


criterion=sequence-density
sequence-density=0.85
sequence-density-rank=1
fanout-score=2.84
fanout-score-rank=30
prefix-density=0.96
prefix-fanout=2.5
sequence=TTCCTCCTGCTTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=190.71
fanout-score-rank=1
prefix-density=0.26
prefix-fanout=10.7
sequence=AAAGAAAAAAAAATGTATTTTGAAGCCATTTTCAGAGAAAAATGCCATTCATTATAACCAAGGAAGTTTTCTTTCAGCCATTCCCGGTGGCTTTTCCTCAGTGGTGCCCACAGAAGGGCAACATAGACCAGCTGTAGATCGCCCATTGGCTCTGGAGGAAGAGAGGTGCAGGTGCTTTGTGCCCGACGGCCACTGTGCCTGAGGGGTGCTGTCCTCACACAGCTGCCTCAGGAGTCTGTCAGAGGGGCCTAGAGTCGGGAGGCCTGGGCAGGGCGTGGGTCCCGGATACAGAGTGCTTGAATGAATCCTTCAGTCGAGGACAGCAATGGCCACACACGGAGCTTCCCACTCACGTCCACCCGTACAGCTGATCCATCACAAGGCAAAACTGGGTCCTCTATAAAACTCCATAAACATCATACATCCTAAAGTCATAGTTACCCAGGACAAAATAAGACAAAAAGATTTTCCTGTATTCATTTTCCACCCAAGTAGACTGTATATTTATTTC
SRR25257911 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 18 13:24:01
                             Started mapping on |	Jun 18 13:24:01
                                    Finished on |	Jun 18 13:26:43
       Mapping speed, Million of reads per hour |	678.61

                          Number of input reads |	30537482
                      Average input read length |	257
                                    UNIQUE READS:
                   Uniquely mapped reads number |	29195636
                        Uniquely mapped reads % |	95.61%
                          Average mapped length |	257.83
                       Number of splices: Total |	31436469
            Number of splices: Annotated (sjdb) |	31235837
                       Number of splices: GT/AG |	31034550
                       Number of splices: GC/AG |	360718
                       Number of splices: AT/AC |	23443
               Number of splices: Non-canonical |	17758
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.97
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.79
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1255130
             % of reads mapped to multiple loci |	4.11%
        Number of reads mapped to too many loci |	74490
             % of reads mapped to too many loci |	0.24%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.04%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	86778	86778	86778
N_multimapping	1255130	1255130	1255130
N_noFeature	853568	27910995	977289
N_ambiguous	2059751	157764	929717
UnstrandedReadsAssigned:26282317 PositiveStrandReadsAssigned:1126877 NegativeStrandReadsAssigned:27288630
Dataset is classified negative stranded
MeadianReadLen=134 20thPercentileLength=106 echo kmer=101
SRR25257911 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25257911-trimmed-pair1.fastq
                             SRR25257911-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 30,537,482 reads, 29,024,982 reads pseudoaligned
[quant] estimated average fragment length: 141.919
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,363 rounds

 109283 SRR25257911.ke.tsv
  52636 SRR25257911.se.tsv
 161919 total
==> SRR25257911.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	10.3333	0	0
ENSMUST00000196221.1	9	3.33333	0	0
ENSMUST00000179664.1	11	5.33333	0	0
ENSMUST00000178537.1	12	6.33333	0	0
ENSMUST00000178862.1	14	8.33333	0	0
ENSMUST00000179520.1	11	5.33333	0	0
ENSMUST00000179883.1	16	10.3333	0	0
ENSMUST00000195858.1	10	4.33333	0	0
ENSMUST00000179932.1	12	6.33333	0	0

==> SRR25257911.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25257911 completed mapping pipeline successfully
