Starting /dee2/code/volunteer_pipeline.sh SRR25257913
    current disk space = 320797776
    free memory = 176866088 
SRR25257913 SRAfilesize
f7f7d86dc933ae7363055f07fb254086  SRR25257913.sra
SRR25257913.sra file validated
SRR25257913 is paired end
SRR25257913 is conventional basespace
SRR25257913 read1 length is 39-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.493	37.0	37.0	37.0	37.0	37.0
2	36.44475	37.0	37.0	37.0	37.0	37.0
3	36.588	37.0	37.0	37.0	37.0	37.0
4	36.599	37.0	37.0	37.0	37.0	37.0
5	36.5945	37.0	37.0	37.0	37.0	37.0
6	36.55	37.0	37.0	37.0	37.0	37.0
7	36.5475	37.0	37.0	37.0	37.0	37.0
8	36.5795	37.0	37.0	37.0	37.0	37.0
9	36.5005	37.0	37.0	37.0	37.0	37.0
10-14	36.574	37.0	37.0	37.0	37.0	37.0
15-19	36.5187	37.0	37.0	37.0	37.0	37.0
20-24	36.48985	37.0	37.0	37.0	37.0	37.0
25-29	36.3966	37.0	37.0	37.0	37.0	37.0
30-34	36.24425000000001	37.0	37.0	37.0	37.0	37.0
35-39	36.37665	37.0	37.0	37.0	37.0	37.0
40-44	36.43165791447863	37.0	37.0	37.0	37.0	37.0
45-49	36.28757764728826	37.0	37.0	37.0	37.0	37.0
50-54	36.12581290645322	37.0	37.0	37.0	37.0	37.0
55-59	36.15525392795852	37.0	37.0	37.0	37.0	37.0
60-64	36.24732889681523	37.0	37.0	37.0	37.0	37.0
65-69	36.2838801231983	37.0	37.0	37.0	37.0	37.0
70-74	36.28119632722312	37.0	37.0	37.0	37.0	37.0
75-79	36.126291541547104	37.0	37.0	37.0	37.0	37.0
80-84	36.18354848067203	37.0	37.0	37.0	37.0	37.0
85-89	36.110738437732465	37.0	37.0	37.0	37.0	37.0
90-94	36.05933343528942	37.0	37.0	37.0	37.0	37.0
95-99	36.010947827768476	37.0	37.0	37.0	37.0	37.0
100-104	35.79093108128303	37.0	37.0	37.0	37.0	37.0
105-109	35.901289414105136	37.0	37.0	37.0	37.0	37.0
110-114	35.85388051054497	37.0	37.0	37.0	37.0	37.0
115-119	35.720335773354684	37.0	37.0	37.0	37.0	37.0
120-124	35.709051002981155	37.0	37.0	37.0	37.0	37.0
125-129	35.809974151180775	37.0	37.0	37.0	37.0	37.0
130-134	35.584300603013254	37.0	37.0	37.0	37.0	37.0
135-139	35.454020699229375	37.0	37.0	37.0	37.0	37.0
140-144	35.62617691568619	37.0	37.0	37.0	37.0	37.0
145-149	35.52142054168524	37.0	37.0	37.0	34.6	37.0
150-151	35.02782743821495	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	2.0
27	8.0
28	8.0
29	17.0
30	22.0
31	39.0
32	43.0
33	69.0
34	95.0
35	520.0
36	2973.0
37	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.174999999999997	12.9	20.25	40.675
2	18.229557389347338	18.479619904976243	40.78519629907477	22.50562640660165
3	21.2	22.650000000000002	30.325000000000003	25.825
4	20.775	30.95	29.575000000000003	18.7
5	22.8	31.125000000000004	31.574999999999996	14.499999999999998
6	16.45	30.775000000000002	35.425000000000004	17.349999999999998
7	11.675	20.625	51.075	16.625
8	18.375	20.375	34.975	26.275
9	19.45	20.474999999999998	40.375	19.7
10-14	20.71	26.31	30.459999999999997	22.52
15-19	20.61	25.965	31.7	21.725
20-24	20.421021051052552	25.281264063203164	31.971598579928994	22.32611630581529
25-29	18.628531356441595	24.479062312161894	31.757162893207774	25.13524343818874
30-34	20.09545340366742	26.24466214518965	31.26350163275559	22.396382818387337
35-39	19.933871048544663	23.52086568809178	31.61665247232103	24.928610791042534
40-44	21.015253813453363	23.475868967241812	31.07776944236059	24.431107776944234
45-49	19.50754726443007	25.3247078882704	30.87106965548368	24.296675191815854
50-54	20.818030889973336	24.747195250792373	30.683704784424208	23.751069074810083
55-59	22.26637159239513	23.584146464138417	29.438688260738356	24.710793682728095
60-64	23.10353472048132	25.72574580095262	28.62371521684633	22.54700426171973
65-69	21.77974187716567	25.71184653241601	29.664038567769797	22.84437302264852
70-74	21.473164837377908	25.148524821266736	29.402879871110667	23.97543047024469
75-79	22.04148027653518	25.218584790565266	30.408702724684833	22.331232208214722
80-84	20.631341600901916	25.82248642000615	30.342318335553962	23.203853643537972
85-89	22.352389878163073	25.330625846089767	29.272102467978755	23.044881807768405
90-94	24.57505195289604	25.37432727660255	28.544786060638355	21.50583470986306
95-99	21.609628931166455	24.859947861778245	29.691053303011817	23.839369904043487
100-104	21.72413793103448	24.856808883693745	28.474576271186443	24.94447691408533
105-109	23.398620349839863	25.08622813500862	27.728504557772848	23.786646957378665
110-114	22.198005415758537	25.711643880853313	27.494881447724723	24.59546925566343
115-119	24.33340481807134	25.477160626206306	27.557366502251767	22.632068053470583
120-124	22.299352624600267	25.146244442711176	30.34864675142345	22.205756181265112
125-129	21.716701902748415	27.027484143763214	28.109936575052853	23.145877378435518
130-134	21.355745384403416	26.922017084596305	29.034628455956646	22.68760907504363
135-139	21.107478545157335	26.256640784634243	28.821005312627708	23.81487535758071
140-144	22.145251396648042	25.977653631284912	28.51396648044693	23.36312849162011
145-149	21.93735791866633	27.039656478908814	28.16367769638798	22.85930790603688
150-151	22.081911262798634	25.870307167235495	28.87372013651877	23.1740614334471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	0.5
22	0.5
23	2.0
24	5.0
25	10.5
26	20.5
27	47.5
28	56.5
29	54.0
30	65.0
31	58.5
32	61.0
33	70.5
34	79.0
35	84.5
36	95.0
37	131.5
38	132.0
39	138.5
40	159.5
41	177.0
42	204.0
43	230.0
44	242.5
45	211.5
46	192.5
47	201.0
48	181.0
49	128.5
50	115.0
51	114.0
52	91.0
53	96.0
54	96.5
55	65.0
56	48.5
57	34.0
58	39.0
59	41.0
60	34.0
61	26.0
62	17.5
63	25.0
64	14.5
65	7.0
66	10.5
67	28.5
68	40.5
69	22.5
70	8.5
71	17.5
72	17.0
73	7.5
74	5.0
75	2.0
76	0.5
77	1.0
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.005
25-29	0.18
30-34	0.475
35-39	0.19499999999999998
40-44	0.0
45-49	0.2501125506477915
50-54	0.5652826413206603
55-59	0.4954706971623042
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.14720064971321253
80-84	0.0
85-89	0.18190322748297905
90-94	0.14366287112908377
95-99	0.29310916933967485
100-104	0.6906959196703233
105-109	0.19056986537161125
110-114	0.16484241065541344
115-119	0.36324786324786323
120-124	0.0389833151411196
125-129	0.22780965237934525
130-134	0.4753633787366304
135-139	0.7704785077047851
140-144	0.311873468478503
145-149	0.7645068304298784
150-151	0.6105834464043419
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	1.0
40-44	0.0
45-49	1.0
50-54	1.0
55-59	4.0
60-64	9.0
65-69	6.0
70-74	23.0
75-79	37.0
80-84	46.0
85-89	67.0
90-94	131.0
95-99	143.0
100-104	193.0
105-109	221.0
110-114	221.0
115-119	246.0
120-124	213.0
125-129	165.0
130-134	217.0
135-139	180.0
140-144	192.0
145-149	197.0
150-152	1486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.09573879358052	27.6
2	16.878804648588822	15.25
3	7.194244604316546	9.75
4	4.482567791920309	8.1
5	2.8223574986164914	6.375
6	1.4941892639734367	4.05
7	1.715550636413946	5.425
8	1.1068068622025455	4.0
9	0.7194244604316548	2.9250000000000003
>10	2.4903154399557277	16.525000000000002
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	29	0.7250000000000001	No Hit
CACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGC	26	0.65	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	25	0.625	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	25	0.625	No Hit
CTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAAT	25	0.625	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	24	0.6	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	24	0.6	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	21	0.525	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	20	0.5	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	18	0.44999999999999996	No Hit
CTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAA	18	0.44999999999999996	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	17	0.42500000000000004	No Hit
GTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCT	16	0.4	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	16	0.4	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	15	0.375	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	15	0.375	No Hit
AATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCAT	15	0.375	No Hit
CTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCT	15	0.375	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	15	0.375	No Hit
CAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTG	15	0.375	No Hit
GAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTC	12	0.3	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	12	0.3	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	12	0.3	No Hit
TCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATTCT	12	0.3	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	12	0.3	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	12	0.3	No Hit
ATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGAA	11	0.27499999999999997	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	11	0.27499999999999997	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	11	0.27499999999999997	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	11	0.27499999999999997	No Hit
ATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGC	11	0.27499999999999997	No Hit
GTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAATC	10	0.25	No Hit
CCTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAA	10	0.25	No Hit
ATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTTT	10	0.25	No Hit
GGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGA	10	0.25	No Hit
CGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTG	10	0.25	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	10	0.25	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	10	0.25	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	10	0.25	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	10	0.25	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	10	0.25	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	10	0.25	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	10	0.25	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	10	0.25	No Hit
TTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCC	10	0.25	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	9	0.22499999999999998	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	9	0.22499999999999998	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	9	0.22499999999999998	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	9	0.22499999999999998	No Hit
CTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAAT	9	0.22499999999999998	No Hit
ACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCA	9	0.22499999999999998	No Hit
GAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAGGT	9	0.22499999999999998	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	9	0.22499999999999998	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	9	0.22499999999999998	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	9	0.22499999999999998	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	9	0.22499999999999998	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	9	0.22499999999999998	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	9	0.22499999999999998	No Hit
CTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAA	8	0.2	No Hit
GCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATC	8	0.2	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	8	0.2	No Hit
GTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGA	8	0.2	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	8	0.2	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	8	0.2	No Hit
TCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATA	8	0.2	No Hit
GTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCT	8	0.2	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	8	0.2	No Hit
TTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAAT	8	0.2	No Hit
GTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGG	8	0.2	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	8	0.2	No Hit
GTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTCGGC	8	0.2	No Hit
GAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTT	8	0.2	No Hit
TGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCC	8	0.2	No Hit
GTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGC	8	0.2	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	8	0.2	No Hit
CCACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATT	8	0.2	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	8	0.2	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	8	0.2	No Hit
GCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCC	7	0.17500000000000002	No Hit
CCCTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCA	7	0.17500000000000002	No Hit
AAAAGATGAAACATATGTATCTCGAGCATTTGTCTCATCCTTTCTGCTGC	7	0.17500000000000002	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	7	0.17500000000000002	No Hit
CAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAGTGTTTTAC	7	0.17500000000000002	No Hit
CTCCCCTCGCCCGGCCACAGCGGACGGGAAGCGAGAGAAGCTGCGCGGAC	7	0.17500000000000002	No Hit
CTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCA	7	0.17500000000000002	No Hit
ATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCA	7	0.17500000000000002	No Hit
CTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGA	7	0.17500000000000002	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	7	0.17500000000000002	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	7	0.17500000000000002	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	7	0.17500000000000002	No Hit
CTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGC	7	0.17500000000000002	No Hit
CTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACAT	7	0.17500000000000002	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	7	0.17500000000000002	No Hit
GCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCAT	7	0.17500000000000002	No Hit
TCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTT	7	0.17500000000000002	No Hit
CTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACT	7	0.17500000000000002	No Hit
TGGGACTGTAGTTACAGTTTTGAGTACCATTGTGTGGGACTGTAGTTCCA	7	0.17500000000000002	No Hit
CCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAGT	7	0.17500000000000002	No Hit
CTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTT	7	0.17500000000000002	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	7	0.17500000000000002	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	7	0.17500000000000002	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	7	0.17500000000000002	No Hit
CCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGA	7	0.17500000000000002	No Hit
GTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGCTCCCT	7	0.17500000000000002	No Hit
TTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGG	7	0.17500000000000002	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	7	0.17500000000000002	No Hit
AATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTT	7	0.17500000000000002	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	7	0.17500000000000002	No Hit
CAGCAAAGAACGGAAGATCGTGTGGGACTGTAGTTCCAGTTTTGAGTACC	7	0.17500000000000002	No Hit
GTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG	6	0.15	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	6	0.15	No Hit
TGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTC	6	0.15	No Hit
CTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCACA	6	0.15	No Hit
CAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAA	6	0.15	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	6	0.15	No Hit
CCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTTTT	6	0.15	No Hit
TTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGC	6	0.15	No Hit
GTGGACTGTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAG	6	0.15	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	6	0.15	No Hit
CTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAA	6	0.15	No Hit
AGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAAT	6	0.15	No Hit
TTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTTTCTG	6	0.15	No Hit
TTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGG	6	0.15	No Hit
GTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTT	6	0.15	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	6	0.15	No Hit
TGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCTCTGCTTGCGA	6	0.15	No Hit
CTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGA	6	0.15	No Hit
CTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGCAAATAGA	6	0.15	No Hit
CTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTA	6	0.15	No Hit
CTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTC	6	0.15	No Hit
CTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTT	6	0.15	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	6	0.15	No Hit
CTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAG	6	0.15	No Hit
CCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTC	6	0.15	No Hit
AAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAA	6	0.15	No Hit
CCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATG	6	0.15	No Hit
CTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTC	5	0.125	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	5	0.125	No Hit
GTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCAT	5	0.125	No Hit
CCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTTCA	5	0.125	No Hit
TTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATTT	5	0.125	No Hit
CCGGGTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTAC	5	0.125	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	5	0.125	No Hit
TTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAG	5	0.125	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	5	0.125	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	5	0.125	No Hit
CAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGA	5	0.125	No Hit
CATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGCAA	5	0.125	No Hit
CCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGA	5	0.125	No Hit
TATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAGGTTGGAAA	5	0.125	No Hit
TTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCTG	5	0.125	No Hit
ACGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACTGG	5	0.125	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	5	0.125	No Hit
CTGTATTCCTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGT	5	0.125	No Hit
GCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTG	5	0.125	No Hit
CTCAAAATGTCAAAATAAAATATATGACAGAGAGGCAATATTAGTTTTCT	5	0.125	No Hit
CCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATC	5	0.125	No Hit
TTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAAT	5	0.125	No Hit
CGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACCAT	5	0.125	No Hit
TGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATT	5	0.125	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	5	0.125	No Hit
GATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGA	5	0.125	No Hit
CATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGA	5	0.125	No Hit
TTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCAAT	5	0.125	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	5	0.125	No Hit
CTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATT	5	0.125	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	5	0.125	No Hit
AATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGC	5	0.125	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	5	0.125	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	5	0.125	No Hit
TTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGATGTATTT	5	0.125	No Hit
TTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATT	5	0.125	No Hit
GAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCA	5	0.125	No Hit
GTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCC	5	0.125	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	5	0.125	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	5	0.125	No Hit
GCATCTTTAAGATTTGATATCCTACTTCGTACTCTGTTTTTGTATTTCAT	5	0.125	No Hit
CTTTAAAGTAAAGCAAAATAGTAAATGATTGTTTGAAATTGTTTTAAATC	5	0.125	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	5	0.125	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	5	0.125	No Hit
GGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCT	5	0.125	No Hit
TCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATATCAAACC	5	0.125	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	5	0.125	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	5	0.125	No Hit
ACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATG	5	0.125	No Hit
AATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTC	5	0.125	No Hit
CTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25257913 read2 length is 39-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257913_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5265	37.0	37.0	37.0	37.0	37.0
2	36.162	37.0	37.0	37.0	37.0	37.0
3	36.59	37.0	37.0	37.0	37.0	37.0
4	36.5665	37.0	37.0	37.0	37.0	37.0
5	36.5055	37.0	37.0	37.0	37.0	37.0
6	36.17375	37.0	37.0	37.0	37.0	37.0
7	36.5135	37.0	37.0	37.0	37.0	37.0
8	36.506	37.0	37.0	37.0	37.0	37.0
9	36.4945	37.0	37.0	37.0	37.0	37.0
10-14	36.229499999999994	37.0	37.0	37.0	37.0	37.0
15-19	36.218450000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.21015	37.0	37.0	37.0	37.0	37.0
25-29	36.4162	37.0	37.0	37.0	37.0	37.0
30-34	36.321450000000006	37.0	37.0	37.0	37.0	37.0
35-39	35.75834999999999	37.0	37.0	37.0	37.0	37.0
40-44	36.365441360340085	37.0	37.0	37.0	37.0	37.0
45-49	36.15330563506309	37.0	37.0	37.0	37.0	37.0
50-54	36.23296648324162	37.0	37.0	37.0	37.0	37.0
55-59	36.22317378408784	37.0	37.0	37.0	37.0	37.0
60-64	36.24097592416423	37.0	37.0	37.0	37.0	37.0
65-69	35.914418606225674	37.0	37.0	37.0	37.0	37.0
70-74	36.17042413647738	37.0	37.0	37.0	37.0	37.0
75-79	35.95897201028291	37.0	37.0	37.0	37.0	37.0
80-84	36.05861837404751	37.0	37.0	37.0	37.0	37.0
85-89	35.96638854466469	37.0	37.0	37.0	37.0	37.0
90-94	35.78370827251187	37.0	37.0	37.0	37.0	37.0
95-99	36.03782879229404	37.0	37.0	37.0	37.0	37.0
100-104	35.93328679675076	37.0	37.0	37.0	37.0	37.0
105-109	35.96281579799637	37.0	37.0	37.0	37.0	37.0
110-114	35.99620892889317	37.0	37.0	37.0	37.0	37.0
115-119	35.92908335138296	37.0	37.0	37.0	37.0	37.0
120-124	35.831785201920965	37.0	37.0	37.0	37.0	37.0
125-129	35.68723336737425	37.0	37.0	37.0	34.6	37.0
130-134	35.68585758356237	37.0	37.0	37.0	37.0	37.0
135-139	35.507448697523486	37.0	37.0	37.0	37.0	37.0
140-144	35.691797146103774	37.0	37.0	37.0	37.0	37.0
145-149	35.60580316618601	37.0	37.0	37.0	34.6	37.0
150-151	35.09426281752472	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	2.0
27	5.0
28	9.0
29	3.0
30	17.0
31	24.0
32	30.0
33	64.0
34	168.0
35	864.0
36	2587.0
37	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.775000000000002	14.099999999999998	21.775	34.35
2	23.484848484848484	23.03030303030303	33.08080808080808	20.404040404040405
3	24.875	26.724999999999998	27.025	21.375
4	28.325	34.35	18.25	19.075
5	26.1	35.075	20.75	18.075
6	22.238993710691823	38.59119496855346	19.82389937106918	19.345911949685537
7	20.9	18.9	39.475	20.724999999999998
8	20.849999999999998	23.625	29.025000000000002	26.5
9	23.1	25.35	29.5	22.05
10-14	24.856307351013413	27.205808208127458	23.182414036502973	24.755470404356156
15-19	24.638484405703633	29.117750793570817	24.149745553484152	22.0940192472414
20-24	22.85210380448476	30.52658100277148	24.812295288485764	21.809019904258
25-29	23.741367230507457	28.90601541387249	24.276849164247825	23.075768191372234
30-34	24.16127576350233	28.689634421543552	24.94358357153603	22.205506243418082
35-39	24.314284260342987	28.53289908910488	25.46435295913694	21.688463691415194
40-44	23.05687797544475	29.54647957905287	26.088699574041595	21.307942871460785
45-49	23.134591194968554	29.51446540880503	25.700628930817608	21.650314465408805
50-54	23.18964219400813	30.325688763988556	24.34887338786571	22.135795654137603
55-59	23.30140901569473	28.80208594494309	25.16672516672517	22.729779872637014
60-64	23.350738025906214	30.249020986042773	24.60588412491214	21.79435686313887
65-69	23.52851864972924	30.214079659901817	24.434434941039527	21.82296674932942
70-74	23.61013015840985	28.412874583795784	26.011502371102814	21.965492886691553
75-79	24.034137367130008	29.420482420278006	25.17886345053148	21.366516762060506
80-84	23.74023035787742	31.02632661456191	25.431921020156313	19.80152200740436
85-89	23.52910412635322	30.568484911876993	25.124208984885726	20.778201976884052
90-94	23.331002205605465	31.405670019904246	24.56829307655065	20.69503469793964
95-99	23.655734975861495	32.445480273014816	24.449253648521168	19.449531102602517
100-104	23.825601024506664	32.09732813318587	23.860527388090112	20.21654345421736
105-109	23.809523809523807	32.66099185788305	22.9706390328152	20.558845299777943
110-114	22.529513198036874	31.80793208648362	26.303223239156388	19.35933147632312
115-119	24.12236710130391	30.656254477718868	25.69852414386015	19.522854277117066
120-124	23.537680593518157	31.80007809449434	23.943771964076532	20.718469347910972
125-129	23.05222241000507	31.561602163258407	25.426736521886088	19.959438904850433
130-134	23.94275754517934	31.015503164847264	25.410512796991103	19.631226492982297
135-139	22.368555385400715	30.484941296579887	26.248085758039817	20.89841755997958
140-144	22.356630824372758	32.336469534050174	25.672043010752688	19.634856630824373
145-149	22.20805711371749	32.24120346761856	24.974502804691483	20.576236613972462
150-151	22.45674740484429	31.591695501730104	27.439446366782004	18.5121107266436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	0.0
21	1.5
22	3.5
23	4.5
24	5.5
25	11.0
26	20.5
27	32.5
28	43.0
29	67.5
30	68.5
31	48.5
32	54.5
33	87.0
34	95.5
35	77.0
36	85.0
37	91.5
38	93.0
39	112.5
40	146.5
41	196.5
42	240.5
43	249.0
44	266.5
45	256.5
46	220.5
47	186.0
48	135.0
49	92.0
50	62.0
51	65.5
52	90.5
53	88.0
54	64.5
55	55.0
56	46.5
57	39.0
58	37.0
59	38.5
60	35.0
61	22.5
62	25.5
63	24.0
64	20.5
65	26.0
66	26.5
67	36.5
68	36.5
69	30.0
70	48.0
71	66.0
72	56.5
73	28.0
74	7.5
75	2.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.0
3	0.0
4	0.0
5	0.0
6	0.625
7	0.0
8	0.0
9	0.0
10-14	0.83
15-19	0.765
20-24	0.775
25-29	0.09
30-34	0.295
35-39	1.745
40-44	0.2000500125031258
45-49	0.5802611175028763
50-54	0.3151575787893947
55-59	0.19018067163805613
60-64	0.19041892162758067
65-69	0.7833291488827517
70-74	0.16620498614958448
75-79	0.659965478728805
80-84	0.27689467746897756
85-89	0.5099120661845049
90-94	0.8216400789628128
95-99	0.17725585775217417
100-104	0.405820627282741
105-109	0.15396932930960153
110-114	0.0927643784786642
115-119	0.12879221522610187
120-124	0.04683475138552806
125-129	0.2528658125421443
130-134	0.23794271071657364
135-139	0.45731707317073167
140-144	0.08952551477170993
145-149	0.2416380516342363
150-151	0.3448275862068966
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	1.0
40-44	0.0
45-49	1.0
50-54	1.0
55-59	4.0
60-64	8.0
65-69	9.0
70-74	22.0
75-79	38.0
80-84	47.0
85-89	69.0
90-94	140.0
95-99	124.0
100-104	199.0
105-109	233.0
110-114	223.0
115-119	236.0
120-124	204.0
125-129	168.0
130-134	223.0
135-139	178.0
140-144	212.0
145-149	198.0
150-152	1462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06025994486018	46.375
2	14.296967309964554	18.15
3	6.2229224103977945	11.85
4	2.520677432059866	6.4
5	1.1815675462780622	3.75
6	0.8270972823946435	3.15
7	0.866482867270579	3.85
8	0.23631350925561243	1.2
9	0.31508467900748327	1.7999999999999998
>10	0.47262701851122485	3.4750000000000005
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	16	0.4	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	14	0.35000000000000003	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	12	0.3	No Hit
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	12	0.3	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	12	0.3	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	11	0.27499999999999997	No Hit
CAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTT	11	0.27499999999999997	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	11	0.27499999999999997	No Hit
CGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGA	10	0.25	No Hit
GAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATT	10	0.25	No Hit
CTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGA	10	0.25	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	10	0.25	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	9	0.22499999999999998	No Hit
GGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATG	9	0.22499999999999998	No Hit
CTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTC	9	0.22499999999999998	No Hit
GTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGG	9	0.22499999999999998	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	9	0.22499999999999998	No Hit
CCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAG	9	0.22499999999999998	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	9	0.22499999999999998	No Hit
CAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAA	9	0.22499999999999998	No Hit
CGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGT	8	0.2	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	8	0.2	No Hit
GCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGCCAAGAAGATGG	8	0.2	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	8	0.2	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	8	0.2	No Hit
TGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCG	8	0.2	No Hit
CTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCG	7	0.17500000000000002	No Hit
GGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAA	7	0.17500000000000002	No Hit
GAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGC	7	0.17500000000000002	No Hit
TTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACT	7	0.17500000000000002	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	7	0.17500000000000002	No Hit
GAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGA	7	0.17500000000000002	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	7	0.17500000000000002	No Hit
TGGGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGG	7	0.17500000000000002	No Hit
GTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGC	7	0.17500000000000002	No Hit
CTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAG	7	0.17500000000000002	No Hit
CTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTA	7	0.17500000000000002	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	7	0.17500000000000002	No Hit
ATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGC	7	0.17500000000000002	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	7	0.17500000000000002	No Hit
GCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCC	7	0.17500000000000002	No Hit
ATTAGATGGACCATCAACTGATAAAGACCCTGAAGAAAAGAAAAAAGAGC	7	0.17500000000000002	No Hit
AAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAG	7	0.17500000000000002	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	7	0.17500000000000002	No Hit
GAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAG	7	0.17500000000000002	No Hit
CCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATT	7	0.17500000000000002	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	7	0.17500000000000002	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	7	0.17500000000000002	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	6	0.15	No Hit
CGCGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGT	6	0.15	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	6	0.15	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	6	0.15	No Hit
ATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGT	6	0.15	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	6	0.15	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	6	0.15	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	6	0.15	No Hit
TGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTG	6	0.15	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	6	0.15	No Hit
CCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAAT	6	0.15	No Hit
GTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGC	6	0.15	No Hit
CACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATG	6	0.15	No Hit
CTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGG	6	0.15	No Hit
TTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCT	6	0.15	No Hit
CCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACG	6	0.15	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	6	0.15	No Hit
ATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAG	6	0.15	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	6	0.15	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	6	0.15	No Hit
TTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAA	6	0.15	No Hit
TGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGC	5	0.125	No Hit
ACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAA	5	0.125	No Hit
GAGACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAG	5	0.125	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	5	0.125	No Hit
GCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGA	5	0.125	No Hit
CATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATG	5	0.125	No Hit
CCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGC	5	0.125	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	5	0.125	No Hit
CGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTG	5	0.125	No Hit
GTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAA	5	0.125	No Hit
CTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCG	5	0.125	No Hit
GGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCT	5	0.125	No Hit
GAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAA	5	0.125	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	5	0.125	No Hit
TTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTC	5	0.125	No Hit
CAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGA	5	0.125	No Hit
TGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAA	5	0.125	No Hit
CGGAAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAA	5	0.125	No Hit
AAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAA	5	0.125	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	5	0.125	No Hit
TGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGG	5	0.125	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	5	0.125	No Hit
TTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCA	5	0.125	No Hit
GGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATT	5	0.125	No Hit
GGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCC	5	0.125	No Hit
TTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATC	5	0.125	No Hit
CTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAAAACACT	5	0.125	No Hit
CTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAG	5	0.125	No Hit
GGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGA	5	0.125	No Hit
CCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0125	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138-139	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Read 4198718 spots for SRR25257913.sra
Written 4198718 spots for SRR25257913.sra
Rejected 466 READS because READLEN < 1
Read 4198725 spots for SRR25257913.sra
Written 4198725 spots for SRR25257913.sra
SRR ids: ['SRR25257913.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_b6tkfqa5
SRR25257913.sra spots: 33589751
blocks: [[1, 4198718], [4198719, 8397436], [8397437, 12596154], [12596155, 16794872], [16794873, 20993590], [20993591, 25192308], [25192309, 29391026], [29391027, 33589751]]
SRR25257913 file size 10075096
SRR25257913 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25257913 SRR25257913_1.fastq SRR25257913_2.fastq
Input file:	SRR25257913_1.fastq
Paired file:	SRR25257913_2.fastq
trimmed:	SRR25257913-trimmed-pair1.fastq, SRR25257913-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu Jun 18 11:47:50 2026 >> started

Thu Jun 18 11:48:54 2026 >> done (64.235s)
33589290 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
33589290 (100.00%) read pairs available; of these:
  270702 ( 0.81%) trimmed read pairs available after processing
33318588 (99.19%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       5	  0.00%
 19	       7	  0.00%
 20	       9	  0.00%
 21	       9	  0.00%
 22	       9	  0.00%
 23	       5	  0.00%
 24	      14	  0.00%
 25	      16	  0.00%
 26	      30	  0.00%
 27	      34	  0.00%
 28	      35	  0.00%
 29	      56	  0.00%
 30	      68	  0.00%
 31	     163	  0.00%
 32	     230	  0.00%
 33	     263	  0.00%
 34	     328	  0.00%
 35	     340	  0.00%
 36	     428	  0.00%
 37	     558	  0.00%
 38	     601	  0.00%
 39	     725	  0.00%
 40	     888	  0.00%
 41	    1079	  0.00%
 42	    1392	  0.00%
 43	    1522	  0.00%
 44	    1827	  0.01%
 45	    2049	  0.01%
 46	    2392	  0.01%
 47	    2587	  0.01%
 48	    2885	  0.01%
 49	    3228	  0.01%
 50	    3662	  0.01%
 51	    4198	  0.01%
 52	    5066	  0.02%
 53	    5678	  0.02%
 54	    6265	  0.02%
 55	    6803	  0.02%
 56	    7586	  0.02%
 57	    8100	  0.02%
 58	    8916	  0.03%
 59	   10005	  0.03%
 60	   11412	  0.03%
 61	   12889	  0.04%
 62	   14061	  0.04%
 63	   16412	  0.05%
 64	   18372	  0.05%
 65	   19119	  0.06%
 66	   21418	  0.06%
 67	   23155	  0.07%
 68	   25044	  0.07%
 69	   27514	  0.08%
 70	   30071	  0.09%
 71	   33427	  0.10%
 72	   37933	  0.11%
 73	   41841	  0.12%
 74	   46413	  0.14%
 75	   51606	  0.15%
 76	   55712	  0.17%
 77	   60858	  0.18%
 78	   63271	  0.19%
 79	   70505	  0.21%
 80	   74756	  0.22%
 81	   80724	  0.24%
 82	   89175	  0.27%
 83	   96390	  0.29%
 84	  104436	  0.31%
 85	  115487	  0.34%
 86	  123533	  0.37%
 87	  130629	  0.39%
 88	  138599	  0.41%
 89	  149745	  0.45%
 90	  153965	  0.46%
 91	  166615	  0.50%
 92	  171343	  0.51%
 93	  187183	  0.56%
 94	  192704	  0.57%
 95	  211299	  0.63%
 96	  219565	  0.65%
 97	  231525	  0.69%
 98	  234303	  0.70%
 99	  241659	  0.72%
100	  258798	  0.77%
101	  261302	  0.78%
102	  268523	  0.80%
103	  279231	  0.83%
104	  294115	  0.88%
105	  296929	  0.88%
106	  309958	  0.92%
107	  320567	  0.95%
108	  325540	  0.97%
109	  334360	  1.00%
110	  324743	  0.97%
111	  326550	  0.97%
112	  334431	  1.00%
113	  333730	  0.99%
114	  338779	  1.01%
115	  346241	  1.03%
116	  346739	  1.03%
117	  359222	  1.07%
118	  355728	  1.06%
119	  355404	  1.06%
120	  349828	  1.04%
121	  348826	  1.04%
122	  348909	  1.04%
123	  344904	  1.03%
124	  349300	  1.04%
125	  345241	  1.03%
126	  339513	  1.01%
127	  347798	  1.04%
128	  354165	  1.05%
129	  334333	  1.00%
130	  341568	  1.02%
131	  328553	  0.98%
132	  322290	  0.96%
133	  327304	  0.97%
134	  312766	  0.93%
135	  318644	  0.95%
136	  312805	  0.93%
137	  318804	  0.95%
138	  303298	  0.90%
139	  308423	  0.92%
140	  306546	  0.91%
141	  298291	  0.89%
142	  302251	  0.90%
143	  288326	  0.86%
144	  288822	  0.86%
145	  290888	  0.87%
146	  368453	  1.10%
147	  439090	  1.31%
148	  346798	  1.03%
149	  262957	  0.78%
150	  350197	  1.04%
151	13133780	 39.10%
33589290 reads passed initial QC


criterion=sequence-density
sequence-density=0.91
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=41
prefix-density=0.92
prefix-fanout=2.0
sequence=AAAAGCAGGAGGAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=117.31
fanout-score-rank=1
prefix-density=0.09
prefix-fanout=7.4
sequence=TGAAGGCAGTTCTGCAACTTTATTTGACACGAGCAGGTTAGTTCTCATCCACGTTGACCGTCTGTAGATTTTTGAATGTGGTAACAGGCACGTAAGTTACCAGCGTGTAGAGCTTGTTTGGGGAATCCTCATCCTCATTACGTTTTCTGGACAAGCGTACTCGGATGCGATATGGAACGTTCCTTATTCCCTTGGCCCAGACGGCTTTATTGAGCCTGGTGTCAATGCGCACATCTGGTGTCCCCATTTCCTTCATGGCAAACTTCCGAATTTCTTTGAGTGCCCGAGGAGCACGCTTCTTGAAGCCCACTCCATGGATGCGCTTGTGAATGTTGATGGTGTATTCTCGGGTCACCACCTCGTTGATGGCAGAACGGCCCTTCTTCTTCTCGCCACCCTTCTTTGCGGGAGCCATTCTGCCGGGC


criterion=sequence-density
sequence-density=0.88
sequence-density-rank=1
fanout-score=2.92
fanout-score-rank=31
prefix-density=1.00
prefix-fanout=2.6
sequence=TTCCTCCTGCTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=35
fanout-score=181.06
fanout-score-rank=1
prefix-density=0.27
prefix-fanout=11.1
sequence=AAAGAAAAAAAAATGTATTTTGAAGCCATTTTCAGAGAAAAATGCCATTCATTATAACCAAGGAAGTTTTCTTTCAGCCATTCCCGGTGGCTTTTCCTCAGTGGTGCCCACAGAAGGGCAACATAGACCAGCTGTAGATCGCCCATTGGCTCTGGAGGAAGAGAGGTGCAGGTGCTTTGTGCCCGACGGCCACTGTGCCTGAGGGGTGCTGTCCTCACACAGCTGCCTCAGGAGTCTGTCAGAGGGGCCTAGAGTCGGGAGGCCTGGGCAGGGCGTGGGTCCCGGATACAGAGTGCTTGAATGAATCCTTCAGTCGAGGACAGCAATGGCCACACACGGAGCTTCCCACTCACGTCCACCCGTACAGCTGATCCATCACAAGGCAAAACTGGGTCCTCTATAAAACTCCATAAACATCATACATCCTAAAGTCATAGTTACCCAGGACAAAATAAGACAAAAAGATTTTCCTGTATTCATTTTCCACCCAAGTAGACTGTATATTTATTTC
SRR25257913 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 18 11:49:19
                             Started mapping on |	Jun 18 11:49:19
                                    Finished on |	Jun 18 11:52:52
       Mapping speed, Million of reads per hour |	567.71

                          Number of input reads |	33589290
                      Average input read length |	261
                                    UNIQUE READS:
                   Uniquely mapped reads number |	32196990
                        Uniquely mapped reads % |	95.85%
                          Average mapped length |	261.95
                       Number of splices: Total |	35162823
            Number of splices: Annotated (sjdb) |	34937509
                       Number of splices: GT/AG |	34690930
                       Number of splices: GC/AG |	425909
                       Number of splices: AT/AC |	25812
               Number of splices: Non-canonical |	20172
                      Mismatch rate per base, % |	0.32%
                         Deletion rate per base |	0.00%
                        Deletion average length |	2.03
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.89
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1300817
             % of reads mapped to multiple loci |	3.87%
        Number of reads mapped to too many loci |	77120
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.04%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	91525	91525	91525
N_multimapping	1300817	1300817	1300817
N_noFeature	992161	30725204	1124055
N_ambiguous	2374405	176792	1068800
UnstrandedReadsAssigned:28830424 PositiveStrandReadsAssigned:1294994 NegativeStrandReadsAssigned:30004135
Dataset is classified negative stranded
MeadianReadLen=138 20thPercentileLength=108 echo kmer=103
SRR25257913 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25257913-trimmed-pair1.fastq
                             SRR25257913-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 33,589,290 reads, 31,853,927 reads pseudoaligned
[quant] estimated average fragment length: 141.619
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,424 rounds

 109283 SRR25257913.ke.tsv
  52636 SRR25257913.se.tsv
 161919 total
==> SRR25257913.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	15	0	0
ENSMUST00000196221.1	9	8	0	0
ENSMUST00000179664.1	11	10	0	0
ENSMUST00000178537.1	12	11	0	0
ENSMUST00000178862.1	14	13	0	0
ENSMUST00000179520.1	11	10	0	0
ENSMUST00000179883.1	16	15	0	0
ENSMUST00000195858.1	10	9	0	0
ENSMUST00000179932.1	12	11	0	0

==> SRR25257913.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25257913 completed mapping pipeline successfully
