Starting /dee2/code/volunteer_pipeline.sh SRR25257916
    current disk space = 428923467776
    free memory = 395577772 
SRR25257916 SRAfilesize
5e93a8f8ec85fb70767e759385ce79a3  SRR25257916.sra
SRR25257916.sra file validated
SRR25257916 is paired end
SRR25257916 is conventional basespace
SRR25257916 read1 length is 45-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	45-151
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.594	37.0	37.0	37.0	37.0	37.0
2	36.504	37.0	37.0	37.0	37.0	37.0
3	36.6675	37.0	37.0	37.0	37.0	37.0
4	36.6225	37.0	37.0	37.0	37.0	37.0
5	36.6425	37.0	37.0	37.0	37.0	37.0
6	36.5415	37.0	37.0	37.0	37.0	37.0
7	36.5635	37.0	37.0	37.0	37.0	37.0
8	36.6	37.0	37.0	37.0	37.0	37.0
9	36.5975	37.0	37.0	37.0	37.0	37.0
10-14	36.613299999999995	37.0	37.0	37.0	37.0	37.0
15-19	36.5479	37.0	37.0	37.0	37.0	37.0
20-24	36.5306	37.0	37.0	37.0	37.0	37.0
25-29	36.42139999999999	37.0	37.0	37.0	37.0	37.0
30-34	36.33325	37.0	37.0	37.0	37.0	37.0
35-39	36.3919	37.0	37.0	37.0	37.0	37.0
40-44	36.494	37.0	37.0	37.0	37.0	37.0
45-49	36.3528608652163	37.0	37.0	37.0	37.0	37.0
50-54	36.2154970206634	37.0	37.0	37.0	37.0	37.0
55-59	36.18000132798631	37.0	37.0	37.0	37.0	37.0
60-64	36.34605130400606	37.0	37.0	37.0	37.0	37.0
65-69	36.3024683447214	37.0	37.0	37.0	37.0	37.0
70-74	36.29018701157946	37.0	37.0	37.0	37.0	37.0
75-79	36.23689417057722	37.0	37.0	37.0	37.0	37.0
80-84	36.19019448235677	37.0	37.0	37.0	37.0	37.0
85-89	36.12968335834092	37.0	37.0	37.0	37.0	37.0
90-94	36.08374377209731	37.0	37.0	37.0	37.0	37.0
95-99	36.12174489678408	37.0	37.0	37.0	37.0	37.0
100-104	35.93614238856933	37.0	37.0	37.0	37.0	37.0
105-109	36.06639079578931	37.0	37.0	37.0	37.0	37.0
110-114	36.03696851347862	37.0	37.0	37.0	37.0	37.0
115-119	35.90379707127292	37.0	37.0	37.0	37.0	37.0
120-124	35.913447485514574	37.0	37.0	37.0	37.0	37.0
125-129	35.9287337113136	37.0	37.0	37.0	37.0	37.0
130-134	35.85314353547366	37.0	37.0	37.0	37.0	37.0
135-139	35.661041888776616	37.0	37.0	37.0	37.0	37.0
140-144	35.902421831312175	37.0	37.0	37.0	37.0	37.0
145-149	35.71797075167665	37.0	37.0	37.0	37.0	37.0
150-151	35.18504046193215	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	2.0
27	6.0
28	8.0
29	16.0
30	15.0
31	27.0
32	56.0
33	58.0
34	106.0
35	444.0
36	2975.0
37	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.5	12.35	20.150000000000002	41.0
2	18.525	17.5	42.0	21.975
3	22.025	21.15	31.624999999999996	25.2
4	21.25	31.75	27.800000000000004	19.2
5	22.5	33.324999999999996	29.5	14.674999999999999
6	16.025	33.5	34.475	16.0
7	11.3	20.349999999999998	50.55	17.8
8	17.0	24.8	35.275	22.925
9	18.45	21.25	39.175	21.125
10-14	20.165	27.105	30.955	21.775
15-19	19.07	27.534999999999997	32.74	20.655
20-24	19.63	26.855	32.025	21.490000000000002
25-29	19.392663860493084	26.062337141711765	31.103427540589294	23.441571457205853
30-34	18.919732945133276	28.025701521007985	31.85081070227398	21.20375483158476
35-39	20.046129161652626	26.659647011632572	30.756117127958284	22.53810669875652
40-44	19.900000000000002	25.355	30.995	23.75
45-49	19.04905206139031	27.49523522921055	31.046243354398634	22.409469355000503
50-54	20.15683120538856	27.847592238865992	29.908515130190004	22.087061425555444
55-59	21.361030077456995	25.13328638969923	30.152902122522885	23.352781410320894
60-64	22.920110192837466	27.002253944402703	29.01076884547959	21.06686701728024
65-69	21.162720706260032	26.21890048154093	29.684991974317814	22.93338683788122
70-74	20.48150381986329	26.734016887816647	29.72456775231202	23.05991154000804
75-79	22.06373292867982	26.61608497723824	30.060698027314114	21.259484066767833
80-84	20.026444263628967	28.061432058584213	30.771969080553298	21.140154597233522
85-89	21.557783750258423	27.305147818896007	29.682654537936738	21.45441389290883
90-94	23.04906848596169	26.63867751246392	29.441091577013907	20.87116242456048
95-99	21.55163131258749	26.461720684828254	29.875094217723703	22.111553784860558
100-104	21.305053444512843	25.256029996082603	29.912138340142146	23.526778219262408
105-109	22.33782841199721	26.801906533364335	28.37712159962799	22.483143455010463
110-114	21.205604030969642	27.14145262381713	27.669902912621357	23.983040432591864
115-119	22.65189748967687	27.44314085337878	28.15101264993118	21.753949007013173
120-124	22.263385991891514	26.02404585488606	29.854606458828464	21.85796169439396
125-129	21.594659465946595	27.782778277827784	28.345334533453347	22.277227722772277
130-134	21.56751499432647	26.462959961095798	30.012968066137134	21.956556978440588
135-139	20.769711581391196	28.395392445754087	28.72577908741852	22.1091168854362
140-144	20.155410590943976	25.88257866462011	30.228319263238678	23.733691481197237
145-149	21.54440983782623	27.73064117710235	27.773601116958435	22.951347868112983
150-151	23.021374927787406	26.97862507221259	28.249566724436743	21.750433275563257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.5
20	1.5
21	1.0
22	5.0
23	13.0
24	16.0
25	32.5
26	49.5
27	63.5
28	74.5
29	87.5
30	91.5
31	57.5
32	54.0
33	80.5
34	94.5
35	100.5
36	132.5
37	164.5
38	136.0
39	146.0
40	179.0
41	187.0
42	198.0
43	225.5
44	237.0
45	179.0
46	168.5
47	175.0
48	133.0
49	110.5
50	95.0
51	91.0
52	88.5
53	78.5
54	78.5
55	62.0
56	41.5
57	31.0
58	28.5
59	38.5
60	46.0
61	29.0
62	16.0
63	20.5
64	12.5
65	4.5
66	5.5
67	22.0
68	31.5
69	15.5
70	3.5
71	7.5
72	7.5
73	1.5
74	1.0
75	1.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.22
30-34	0.395
35-39	0.27999999999999997
40-44	0.0
45-49	0.2900580116023205
50-54	0.43541364296081275
55-59	0.4805285814395836
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.09601293647986256
80-84	0.0
85-89	0.23718675879137877
90-94	0.1310272536687631
95-99	0.2577596391365052
100-104	0.6670743231975096
105-109	0.11611704598235022
110-114	0.13500245459008345
115-119	0.4047261570598603
120-124	0.09078846288148613
125-129	0.2543767768966033
130-134	0.5561376642218102
135-139	0.9551605200318386
140-144	0.39178213091256564
145-149	0.8413205537806177
150-151	0.6029285099052542
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	3.0
50-54	1.0
55-59	2.0
60-64	5.0
65-69	5.0
70-74	16.0
75-79	19.0
80-84	49.0
85-89	57.0
90-94	72.0
95-99	123.0
100-104	145.0
105-109	162.0
110-114	202.0
115-119	204.0
120-124	189.0
125-129	193.0
130-134	204.0
135-139	188.0
140-144	196.0
145-149	205.0
150-152	1760.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.574999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.90565002742732	29.125
2	14.042786615469009	12.8
3	7.021393307734504	9.6
4	4.991771804717499	9.1
5	2.578167855183763	5.875
6	1.3165112452002194	3.5999999999999996
7	1.3165112452002194	4.2
8	1.2616566099835436	4.6
9	0.7679648930334613	3.15
>10	2.797586396050466	17.95
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	35	0.8750000000000001	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	25	0.625	No Hit
CTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAAATA	22	0.5499999999999999	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	22	0.5499999999999999	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	20	0.5	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	20	0.5	No Hit
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	18	0.44999999999999996	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	18	0.44999999999999996	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	18	0.44999999999999996	No Hit
GTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAATC	17	0.42500000000000004	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	16	0.4	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	16	0.4	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	16	0.4	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	16	0.4	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	15	0.375	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	15	0.375	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	15	0.375	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	15	0.375	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	14	0.35000000000000003	No Hit
CTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAA	14	0.35000000000000003	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	14	0.35000000000000003	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	14	0.35000000000000003	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	14	0.35000000000000003	No Hit
TAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCA	14	0.35000000000000003	No Hit
GCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATC	13	0.325	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	13	0.325	No Hit
TGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTG	12	0.3	No Hit
CTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGA	12	0.3	No Hit
TTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATAT	12	0.3	No Hit
CTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAAT	12	0.3	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	11	0.27499999999999997	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	11	0.27499999999999997	No Hit
CTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGA	11	0.27499999999999997	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	11	0.27499999999999997	No Hit
GTGGACTGTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAG	11	0.27499999999999997	No Hit
GCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTG	11	0.27499999999999997	No Hit
GGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATG	11	0.27499999999999997	No Hit
CGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCT	11	0.27499999999999997	No Hit
CATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGA	11	0.27499999999999997	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	11	0.27499999999999997	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	11	0.27499999999999997	No Hit
TAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTT	10	0.25	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	10	0.25	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	10	0.25	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	10	0.25	No Hit
AATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCAT	10	0.25	No Hit
TCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTT	10	0.25	No Hit
GTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCC	10	0.25	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	10	0.25	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	10	0.25	No Hit
ATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGC	10	0.25	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	9	0.22499999999999998	No Hit
GAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACT	9	0.22499999999999998	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	9	0.22499999999999998	No Hit
CTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTG	9	0.22499999999999998	No Hit
CCTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAA	9	0.22499999999999998	No Hit
CCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGA	9	0.22499999999999998	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	9	0.22499999999999998	No Hit
TGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATT	9	0.22499999999999998	No Hit
TTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAAT	9	0.22499999999999998	No Hit
CTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCT	9	0.22499999999999998	No Hit
CACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAGTGTTTT	9	0.22499999999999998	No Hit
CTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTA	9	0.22499999999999998	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	9	0.22499999999999998	No Hit
CTCAAAAGCATTATCCCCATTATTAGGGATAATGTAATACCATCTGATTC	9	0.22499999999999998	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	8	0.2	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	8	0.2	No Hit
TTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAG	8	0.2	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	8	0.2	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	8	0.2	No Hit
TCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCAC	8	0.2	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	8	0.2	No Hit
TTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGC	8	0.2	No Hit
CTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCG	8	0.2	No Hit
TCTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGG	8	0.2	No Hit
CTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAA	8	0.2	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	8	0.2	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	8	0.2	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	8	0.2	No Hit
GGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGG	8	0.2	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	8	0.2	No Hit
GAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTT	8	0.2	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	8	0.2	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	8	0.2	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	8	0.2	No Hit
GTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGT	8	0.2	No Hit
CCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTC	8	0.2	No Hit
CTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCC	8	0.2	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	7	0.17500000000000002	No Hit
TTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAG	7	0.17500000000000002	No Hit
TGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCAC	7	0.17500000000000002	No Hit
GTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGA	7	0.17500000000000002	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	7	0.17500000000000002	No Hit
ATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCA	7	0.17500000000000002	No Hit
CAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGAGC	7	0.17500000000000002	No Hit
CGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTG	7	0.17500000000000002	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	7	0.17500000000000002	No Hit
TCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATTCT	7	0.17500000000000002	No Hit
CAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCA	7	0.17500000000000002	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	7	0.17500000000000002	No Hit
TCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGA	7	0.17500000000000002	No Hit
ATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAA	7	0.17500000000000002	No Hit
CCACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATAT	7	0.17500000000000002	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	7	0.17500000000000002	No Hit
CAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTG	7	0.17500000000000002	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	7	0.17500000000000002	No Hit
CTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTC	7	0.17500000000000002	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	7	0.17500000000000002	No Hit
CCACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATT	7	0.17500000000000002	No Hit
AATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTT	7	0.17500000000000002	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	7	0.17500000000000002	No Hit
AATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTC	7	0.17500000000000002	No Hit
GCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCC	6	0.15	No Hit
CTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTC	6	0.15	No Hit
GAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGC	6	0.15	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	6	0.15	No Hit
CTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCA	6	0.15	No Hit
GGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAG	6	0.15	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	6	0.15	No Hit
CCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCC	6	0.15	No Hit
CCAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAG	6	0.15	No Hit
GTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCT	6	0.15	No Hit
CTACTTCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTG	6	0.15	No Hit
CACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATG	6	0.15	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	6	0.15	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	6	0.15	No Hit
CTCGAGCATTTGTCTCATCCTTTCTGCTGCTTACATTGCTGCTGGAACTA	6	0.15	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	6	0.15	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	6	0.15	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	6	0.15	No Hit
ATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCT	6	0.15	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	6	0.15	No Hit
TGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAAA	6	0.15	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	6	0.15	No Hit
GGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAA	6	0.15	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	6	0.15	No Hit
CCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATCT	5	0.125	No Hit
GTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG	5	0.125	No Hit
AGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGCT	5	0.125	No Hit
GGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTC	5	0.125	No Hit
TACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGAT	5	0.125	No Hit
GCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAAT	5	0.125	No Hit
TGCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGA	5	0.125	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	5	0.125	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	5	0.125	No Hit
GCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCC	5	0.125	No Hit
GCCAGCCGCATTCTTTTTCTGCACCATTTTGTCCATCTTCTTGGCAATGC	5	0.125	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	5	0.125	No Hit
CTTCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATA	5	0.125	No Hit
CCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTT	5	0.125	No Hit
CCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGC	5	0.125	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	5	0.125	No Hit
ATTTAATAAGTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCACTTTT	5	0.125	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	5	0.125	No Hit
CTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAAT	5	0.125	No Hit
TCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTCATCAGC	5	0.125	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	5	0.125	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	5	0.125	No Hit
CATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCC	5	0.125	No Hit
CAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTAAACTTAAAATC	5	0.125	No Hit
CTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAA	5	0.125	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
TGGGACTGTAGTTACAGTTTTGAGTACCATTGTGTGGGACTGTAGTTCCA	5	0.125	No Hit
CACCACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATAT	5	0.125	No Hit
TGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATAGCCAT	5	0.125	No Hit
CCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTAT	5	0.125	No Hit
GAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAGGT	5	0.125	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	5	0.125	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	5	0.125	No Hit
TCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTA	5	0.125	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	5	0.125	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	5	0.125	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	5	0.125	No Hit
GCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATTTGATATC	5	0.125	No Hit
CCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGA	5	0.125	No Hit
TGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCA	5	0.125	No Hit
CCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGC	5	0.125	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	5	0.125	No Hit
CCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGAC	5	0.125	No Hit
TGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCA	5	0.125	No Hit
TGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCTCTGCTTG	5	0.125	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0125	0.0	0.0	0.0	0.0
106-107	0.025	0.0	0.0	0.0	0.0
108-109	0.025	0.0	0.0	0.0	0.0
110-111	0.025	0.0	0.0	0.0	0.0
112-113	0.025	0.0	0.0	0.0	0.0
114-115	0.025	0.0	0.0	0.0	0.0
116-117	0.025	0.0	0.0	0.0	0.0
118-119	0.025	0.0	0.0	0.0	0.0
120-121	0.025	0.0	0.0	0.0	0.0
122-123	0.025	0.0	0.0	0.0	0.0
124-125	0.025	0.0	0.0	0.0	0.0
126-127	0.025	0.0	0.0	0.0	0.0
128-129	0.025	0.0	0.0	0.0	0.0
130-131	0.025	0.0	0.0	0.0	0.0
132-133	0.025	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138-139	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25257916 read2 length is 45-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257916_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	45-151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5305	37.0	37.0	37.0	37.0	37.0
2	36.207	37.0	37.0	37.0	37.0	37.0
3	36.5885	37.0	37.0	37.0	37.0	37.0
4	36.3825	37.0	37.0	37.0	37.0	37.0
5	36.449	37.0	37.0	37.0	37.0	37.0
6	36.0795	37.0	37.0	37.0	37.0	37.0
7	36.5075	37.0	37.0	37.0	37.0	37.0
8	36.5135	37.0	37.0	37.0	37.0	37.0
9	36.556	37.0	37.0	37.0	37.0	37.0
10-14	36.2893	37.0	37.0	37.0	37.0	37.0
15-19	36.147000000000006	37.0	37.0	37.0	37.0	37.0
20-24	36.21835	37.0	37.0	37.0	37.0	37.0
25-29	36.428450000000005	37.0	37.0	37.0	37.0	37.0
30-34	36.3326	37.0	37.0	37.0	37.0	37.0
35-39	35.71925	37.0	37.0	37.0	37.0	37.0
40-44	36.34085	37.0	37.0	37.0	37.0	37.0
45-49	36.15401707926982	37.0	37.0	37.0	37.0	37.0
50-54	36.1774603850786	37.0	37.0	37.0	37.0	37.0
55-59	36.1343972884023	37.0	37.0	37.0	37.0	37.0
60-64	36.18829313398588	37.0	37.0	37.0	37.0	37.0
65-69	35.907694898734086	37.0	37.0	37.0	37.0	37.0
70-74	36.11408254330497	37.0	37.0	37.0	37.0	37.0
75-79	35.86790362506352	37.0	37.0	37.0	37.0	37.0
80-84	36.04592746179507	37.0	37.0	37.0	37.0	37.0
85-89	35.92168267773013	37.0	37.0	37.0	37.0	37.0
90-94	35.780021034381186	37.0	37.0	37.0	37.0	37.0
95-99	36.0515040285258	37.0	37.0	37.0	37.0	37.0
100-104	35.88807976459004	37.0	37.0	37.0	37.0	37.0
105-109	35.924887853697825	37.0	37.0	37.0	37.0	37.0
110-114	35.926566054324255	37.0	37.0	37.0	37.0	37.0
115-119	35.86122760054627	37.0	37.0	37.0	37.0	37.0
120-124	35.808808495538116	37.0	37.0	37.0	37.0	37.0
125-129	35.71236251228932	37.0	37.0	37.0	37.0	37.0
130-134	35.567837198680145	37.0	37.0	37.0	37.0	37.0
135-139	35.41330582642485	37.0	37.0	37.0	34.6	37.0
140-144	35.53558686635899	37.0	37.0	37.0	37.0	37.0
145-149	35.28042023107409	37.0	37.0	37.0	32.2	37.0
150-151	34.86736014904696	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	0.0
26	2.0
27	8.0
28	13.0
29	11.0
30	12.0
31	23.0
32	42.0
33	69.0
34	179.0
35	879.0
36	2531.0
37	230.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.975	13.925	20.849999999999998	34.25
2	23.37695017614494	24.00603925515853	33.945646703573225	18.671363865123304
3	24.85	24.25	28.025	22.875
4	27.125	32.45	20.8	19.625
5	26.424999999999997	35.175	19.35	19.05
6	22.040302267002517	39.269521410579344	20.35264483627204	18.337531486146098
7	20.3	18.825	38.75	22.125
8	21.575	23.875	28.449999999999996	26.1
9	21.65	25.2	30.15	23.0
10-14	24.982357092448837	27.522935779816514	23.732231071680612	23.762476056054037
15-19	24.3334679862654	28.645728135730153	25.13633609371844	21.884467784286002
20-24	22.990183740246664	30.480745028945382	25.930027686886486	20.599043543921468
25-29	22.4941200020017	29.470049542110793	26.432467597457837	21.603362858429666
30-34	22.7696471531676	28.598636728147554	26.33319967923015	22.29851643945469
35-39	23.407181054239878	28.16399286987522	26.60555131143366	21.823274764451234
40-44	23.033004457354636	28.577152301297144	27.02459057444784	21.365252666900385
45-49	21.97625276715637	29.67900986113906	26.47413966592876	21.87059770577581
50-54	22.28534084053891	31.032575909913536	25.45747033983511	21.224612909712448
55-59	21.813344043375672	29.675184497213714	26.442090466388873	22.06938099302174
60-64	22.876506024096386	29.698795180722893	25.988955823293175	21.43574297188755
65-69	22.279057989364397	29.779691061028107	26.45226639655609	21.488984553051406
70-74	23.24433249370277	28.89168765743073	27.093198992443323	20.770780856423173
75-79	22.534637326813368	30.34841075794621	25.891401792991037	21.22555012224939
80-84	21.85825807439155	30.86892188376958	27.542221541915403	19.730598499923467
85-89	22.4153989444272	30.192486805339957	26.999896512470244	20.3922177377626
90-94	21.73798203909139	30.87691494981511	26.798732171156892	20.586370839936606
95-99	23.100958948389184	30.67018640232734	27.060661566641524	19.168193082641956
100-104	21.9870434491232	31.749134368368143	25.924271194013183	20.339550988495475
105-109	23.129330926454315	30.84493099633145	25.56338438246084	20.46235369475339
110-114	21.801269019897738	32.31688535698885	26.64941785252264	19.23242777059077
115-119	22.72489197328794	32.080659945004584	26.66623019510279	18.528217886604686
120-124	22.171755991894347	32.506463559499686	25.784361679826706	19.53741876877926
125-129	22.287257991895544	31.09710340687378	26.932312772024613	19.683325829206062
130-134	23.071932527775523	30.889627767415455	26.283350904225124	19.755088800583895
135-139	21.681810083734188	31.177623374309636	26.340637805095312	20.79992873686086
140-144	21.269197334106057	32.657200811359026	27.692456292861973	18.381145561672945
145-149	21.586334457621586	32.35774126863235	26.036339897726034	20.01958437602002
150-151	21.346998535871155	32.09370424597365	27.437774524158126	19.121522693997072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	2.0
21	3.5
22	5.5
23	8.0
24	14.5
25	27.0
26	44.5
27	75.0
28	84.5
29	77.5
30	72.5
31	62.5
32	70.5
33	92.0
34	101.5
35	92.0
36	105.0
37	121.5
38	104.0
39	107.5
40	129.0
41	169.5
42	234.0
43	238.5
44	215.0
45	197.5
46	184.0
47	175.0
48	135.0
49	92.5
50	72.5
51	68.5
52	71.5
53	76.5
54	61.0
55	37.5
56	33.5
57	44.5
58	50.0
59	42.0
60	39.5
61	33.5
62	27.0
63	20.5
64	17.5
65	29.5
66	33.5
67	35.0
68	33.0
69	27.5
70	41.5
71	57.5
72	44.0
73	18.0
74	5.0
75	1.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.65
3	0.0
4	0.0
5	0.0
6	0.75
7	0.0
8	0.0
9	0.0
10-14	0.8099999999999999
15-19	0.98
20-24	0.675
25-29	0.08499999999999999
30-34	0.24
35-39	1.825
40-44	0.165
45-49	0.6001200240048009
50-54	0.4454231519943947
55-59	0.29532485734307734
60-64	0.2004008016032064
65-69	0.9232776356064027
70-74	0.1910699919549477
75-79	0.7582650894752806
80-84	0.28998778998779
85-89	0.46353522867737945
90-94	0.8485229415461975
95-99	0.16135972461273665
100-104	0.3838664812239221
105-109	0.17438818810672557
110-114	0.12305420537746876
115-119	0.150356279008956
120-124	0.027942717429269997
125-129	0.25449101796407186
130-134	0.41993054994750867
135-139	0.5404447594577833
140-144	0.16393442622950818
145-149	0.3361526783777922
150-151	0.2628504672897196
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	3.0
50-54	1.0
55-59	3.0
60-64	5.0
65-69	6.0
70-74	15.0
75-79	20.0
80-84	45.0
85-89	55.0
90-94	76.0
95-99	129.0
100-104	138.0
105-109	172.0
110-114	199.0
115-119	198.0
120-124	188.0
125-129	201.0
130-134	200.0
135-139	195.0
140-144	220.0
145-149	201.0
150-152	1730.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.949999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67396403440188	46.475
2	14.659890539483971	18.75
3	5.199374511336982	9.975000000000001
4	3.9093041438623923	10.0
5	1.5246286161063332	4.875
6	0.6645817044566067	2.55
7	0.547302580140735	2.45
8	0.3518373729476153	1.7999999999999998
9	0.1954652071931196	1.125
>10	0.2736512900703675	2.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	13	0.325	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	13	0.325	No Hit
GGAGGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGA	12	0.3	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	11	0.27499999999999997	No Hit
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	11	0.27499999999999997	No Hit
CTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAA	10	0.25	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	10	0.25	No Hit
GAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATA	9	0.22499999999999998	No Hit
TGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTG	9	0.22499999999999998	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	9	0.22499999999999998	No Hit
TTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCT	9	0.22499999999999998	No Hit
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	9	0.22499999999999998	No Hit
AGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAG	8	0.2	No Hit
GGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGC	8	0.2	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	8	0.2	No Hit
CGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGT	8	0.2	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	8	0.2	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	8	0.2	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	8	0.2	No Hit
CTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGA	8	0.2	No Hit
CAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCA	8	0.2	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	7	0.17500000000000002	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	7	0.17500000000000002	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	7	0.17500000000000002	No Hit
GGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTT	7	0.17500000000000002	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	7	0.17500000000000002	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	7	0.17500000000000002	No Hit
CCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAA	7	0.17500000000000002	No Hit
GTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGG	7	0.17500000000000002	No Hit
TGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTA	7	0.17500000000000002	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	7	0.17500000000000002	No Hit
CGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGG	7	0.17500000000000002	No Hit
CTGGCTTCAAAATATAATTTCTTAAATATCTTATAGCCGATTTTAAGTTT	7	0.17500000000000002	No Hit
GACGCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCC	7	0.17500000000000002	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	7	0.17500000000000002	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	6	0.15	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	6	0.15	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	6	0.15	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	6	0.15	No Hit
CGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGG	6	0.15	No Hit
TTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACT	6	0.15	No Hit
TGGAGGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAG	6	0.15	No Hit
GTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAA	6	0.15	No Hit
GCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGG	6	0.15	No Hit
CAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGA	6	0.15	No Hit
CGGAAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAA	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
CCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAG	6	0.15	No Hit
CACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATG	6	0.15	No Hit
CTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGG	6	0.15	No Hit
GGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAA	6	0.15	No Hit
GGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGA	6	0.15	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	5	0.125	No Hit
CGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGG	5	0.125	No Hit
CGCAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTT	5	0.125	No Hit
CATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATG	5	0.125	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	5	0.125	No Hit
GTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTC	5	0.125	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	5	0.125	No Hit
CCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAA	5	0.125	No Hit
ATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAA	5	0.125	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	5	0.125	No Hit
CGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCC	5	0.125	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	5	0.125	No Hit
CTTCGGGCTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATAT	5	0.125	No Hit
CTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGA	5	0.125	No Hit
CTTAAATATCTTATAGCCGATTTTAAGTTTACATTTTCTGTACATGTTAT	5	0.125	No Hit
TTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAAT	5	0.125	No Hit
GGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCT	5	0.125	No Hit
ACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCG	5	0.125	No Hit
GGGAGGCAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGA	5	0.125	No Hit
GCCAAATGTCTGGATTTAAAACAATTTCAAACAATCATTTACTATTTTGC	5	0.125	No Hit
GCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGA	5	0.125	No Hit
GTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATT	5	0.125	No Hit
CAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCA	5	0.125	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	5	0.125	No Hit
CTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTA	5	0.125	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	5	0.125	No Hit
CCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACG	5	0.125	No Hit
GTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAA	5	0.125	No Hit
GAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAA	5	0.125	No Hit
TTCTGTTTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGATGG	5	0.125	No Hit
CTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAAT	5	0.125	No Hit
ACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCAT	5	0.125	No Hit
ATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTAGTCCA	5	0.125	No Hit
CTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAG	5	0.125	No Hit
CAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTCGCTTCCCG	5	0.125	No Hit
GTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCC	5	0.125	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	5	0.125	No Hit
AGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAA	5	0.125	No Hit
CAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Read 2157752 spots for SRR25257916.sra
Written 2157752 spots for SRR25257916.sra
Rejected 502 READS because READLEN < 1
Read 2157767 spots for SRR25257916.sra
Written 2157767 spots for SRR25257916.sra
SRR ids: ['SRR25257916.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_w9m2tpxz
SRR25257916.sra spots: 34524047
blocks: [[1, 2157752], [2157753, 4315504], [4315505, 6473256], [6473257, 8631008], [8631009, 10788760], [10788761, 12946512], [12946513, 15104264], [15104265, 17262016], [17262017, 19419768], [19419769, 21577520], [21577521, 23735272], [23735273, 25893024], [25893025, 28050776], [28050777, 30208528], [30208529, 32366280], [32366281, 34524047]]
SRR25257916 file size 1499719
SRR25257916 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25257916 SRR25257916_1.fastq SRR25257916_2.fastq
Input file:	SRR25257916_1.fastq
Paired file:	SRR25257916_2.fastq
trimmed:	SRR25257916-trimmed-pair1.fastq, SRR25257916-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Thu Jun 18 14:16:10 2026 >> started

Thu Jun 18 14:17:37 2026 >> done (86.935s)
34523557 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
34523557 (100.00%) read pairs available; of these:
  280371 ( 0.81%) trimmed read pairs available after processing
34243186 (99.19%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       6	  0.00%
 19	       8	  0.00%
 20	      12	  0.00%
 21	       5	  0.00%
 22	      15	  0.00%
 23	      13	  0.00%
 24	       9	  0.00%
 25	      25	  0.00%
 26	      24	  0.00%
 27	      31	  0.00%
 28	      49	  0.00%
 29	      70	  0.00%
 30	     103	  0.00%
 31	     192	  0.00%
 32	     267	  0.00%
 33	     244	  0.00%
 34	     329	  0.00%
 35	     349	  0.00%
 36	     459	  0.00%
 37	     503	  0.00%
 38	     620	  0.00%
 39	     679	  0.00%
 40	     814	  0.00%
 41	     909	  0.00%
 42	    1021	  0.00%
 43	    1114	  0.00%
 44	    1262	  0.00%
 45	    1419	  0.00%
 46	    1584	  0.00%
 47	    1716	  0.00%
 48	    2017	  0.01%
 49	    2388	  0.01%
 50	    2906	  0.01%
 51	    3178	  0.01%
 52	    3784	  0.01%
 53	    3850	  0.01%
 54	    3975	  0.01%
 55	    4137	  0.01%
 56	    4666	  0.01%
 57	    4977	  0.01%
 58	    5927	  0.02%
 59	    6630	  0.02%
 60	    7674	  0.02%
 61	    8770	  0.03%
 62	    9380	  0.03%
 63	   10463	  0.03%
 64	   11572	  0.03%
 65	   11551	  0.03%
 66	   12700	  0.04%
 67	   13419	  0.04%
 68	   14996	  0.04%
 69	   16301	  0.05%
 70	   18316	  0.05%
 71	   20422	  0.06%
 72	   22823	  0.07%
 73	   25033	  0.07%
 74	   28022	  0.08%
 75	   31535	  0.09%
 76	   33816	  0.10%
 77	   36680	  0.11%
 78	   38414	  0.11%
 79	   43344	  0.13%
 80	   46532	  0.13%
 81	   50872	  0.15%
 82	   55958	  0.16%
 83	   61945	  0.18%
 84	   67421	  0.20%
 85	   73863	  0.21%
 86	   80988	  0.23%
 87	   87645	  0.25%
 88	   92114	  0.27%
 89	  100899	  0.29%
 90	  105467	  0.31%
 91	  114734	  0.33%
 92	  119843	  0.35%
 93	  133113	  0.39%
 94	  136052	  0.39%
 95	  151329	  0.44%
 96	  160490	  0.46%
 97	  170040	  0.49%
 98	  176021	  0.51%
 99	  184239	  0.53%
100	  195397	  0.57%
101	  201242	  0.58%
102	  207209	  0.60%
103	  217734	  0.63%
104	  229044	  0.66%
105	  236781	  0.69%
106	  248237	  0.72%
107	  258785	  0.75%
108	  266508	  0.77%
109	  277443	  0.80%
110	  272460	  0.79%
111	  277670	  0.80%
112	  285330	  0.83%
113	  286039	  0.83%
114	  294718	  0.85%
115	  302043	  0.87%
116	  305352	  0.88%
117	  319504	  0.93%
118	  318693	  0.92%
119	  319432	  0.93%
120	  322103	  0.93%
121	  321980	  0.93%
122	  324264	  0.94%
123	  319945	  0.93%
124	  327149	  0.95%
125	  326721	  0.95%
126	  322583	  0.93%
127	  329799	  0.96%
128	  337163	  0.98%
129	  326056	  0.94%
130	  334155	  0.97%
131	  327658	  0.95%
132	  323654	  0.94%
133	  328378	  0.95%
134	  312833	  0.91%
135	  321584	  0.93%
136	  315530	  0.91%
137	  323638	  0.94%
138	  308880	  0.89%
139	  315261	  0.91%
140	  316987	  0.92%
141	  312404	  0.90%
142	  318278	  0.92%
143	  303610	  0.88%
144	  308766	  0.89%
145	  314537	  0.91%
146	  411200	  1.19%
147	  498375	  1.44%
148	  384064	  1.11%
149	  278985	  0.81%
150	  391589	  1.13%
151	16248725	 47.07%
34523557 reads passed initial QC


criterion=sequence-density
sequence-density=0.55
sequence-density-rank=1
fanout-score=2.78
fanout-score-rank=26
prefix-density=0.61
prefix-fanout=2.5
sequence=GGTGTTCAGGCCCGTGTAGACAGCATCTGCTTTCTCACGGCTGGCTATAGCTGCCTTTCGGACCTGGATCTTGAGTCGACAGTAGAGTAGGGTAAGGACAATACCATACAAAAACAGGACAGCATCCAGGATATAGCAGAGCTGCGGCTCTCCCAGGGCGGCTGCTTGTTCCACCAAAAGGAGCAAGAACAAGATCACGGCTGAGATCATCCTGGGTGCGCTGGGGGCTGCGGCGCTGGGAGCTGGTGATCGCGCTGCGCTGACAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=327.89
fanout-score-rank=1
prefix-density=0.35
prefix-fanout=7.8
sequence=TTCTTCATCTACCTCATTGTCAGCCTCCTGCTCCCCATTTTCCTCATTTTGAGCGTTCCCATTGGCAGGTGCATCTCTTCCATTCTCTGCCTCCTCCACAACTTCCTTCTTCTCCTTCAAGTCCTTGGTGGTGATCTCGGAGCTGGTATCCACTGCCGCGTCTGACATGGTGGGGCACGCCGGTGGTCCGATGCCGCGAGTGAGGGAATTAAAAGTCGAGTTCGAGGACTCTGGCGAGAGAGCTGCCGGAGTCCGCGCTGGAGGAGGCGGTGGGCGGCGAAGGTGGCTGCAGCGAGCGCGGAGCACGACCCAGGAACAATGCAAAGATGGCTTTTCG


criterion=sequence-density
sequence-density=0.53
sequence-density-rank=1
fanout-score=3.87
fanout-score-rank=16
prefix-density=0.67
prefix-fanout=3.0
sequence=GGAGCCAGGAGACATATGAGACTCTGAAGCATGAGAAACCACCCCAGTAGCTTCAGAACAGACGTGCTTGGCTGCATTCTTTTCCCACTTCTAATTCTCTCCGAGCCCTCTTGGTCACCTCTGTGCTTTGAAGGTTGGCTGACCTTATTCACATAATGATGCTAGCTAGGCTCTACATCAGTGTACACTGGCAGGTCCCCATCTCCGTTAAAGACTTACTCACTGACATTTCTCTTCTTCCAGCCTCCTTTGCTTCATTTCTTTTTCCTTCCCTGATCCTCGACTCTCACTAAACAATGGAAAGGGATTATCCCCCAATAAAGCTGCCAGAGACCTG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=41.46
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=7.2
sequence=TGCTCCTGAGCTCGCGCGTTCCCTGCTTGCTGCCCTTCTGGCGCCCGCGCTGGTGGCACTACTGGTGTCGCCGGCCTCGGGTCGCGGAGGCCGGGACCACGGGGACTGGGACGTGGACAGGCGATTGCCTCCGCTGCCACCCCGCGAAGACGGGCCGCGCGTGGCCCGCTTCGTGACTCACGTCTCAGACTGGGGCTCGCTGGCCACTATCTCCACAATAAAGGAGGTGCGCGGCTGGCCCTTCGCGGACATCATCTCAATCAGTGACGGTCCTCCGGGAGAAGGCACGGGCGAGCCCTACATGTACCTGAGTCCACTGCAGCAAGCCGTGAGCGACCTGCAGGAAAATCCAGAGGCTACGCTGACTATGTCTTTAGCACAGACTGTCTACTGTAGGAATCATGGATTTGATCCCCAGAGTCCCCTGTGTGTTCATATAATGATGTCGGGAACTGTGACCAAGGTGAACAAGACAGAAGAGGACTATGCAAGGGATTCGCTGTTTGTTCGA
SRR25257916 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 18 14:18:23
                             Started mapping on |	Jun 18 14:18:23
                                    Finished on |	Jun 18 14:25:17
       Mapping speed, Million of reads per hour |	300.20

                          Number of input reads |	34523557
                      Average input read length |	270
                                    UNIQUE READS:
                   Uniquely mapped reads number |	33107497
                        Uniquely mapped reads % |	95.90%
                          Average mapped length |	270.35
                       Number of splices: Total |	35711496
            Number of splices: Annotated (sjdb) |	35486184
                       Number of splices: GT/AG |	35382417
                       Number of splices: GC/AG |	275073
                       Number of splices: AT/AC |	30224
               Number of splices: Non-canonical |	23782
                      Mismatch rate per base, % |	0.33%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.90
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1318037
             % of reads mapped to multiple loci |	3.82%
        Number of reads mapped to too many loci |	79830
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.05%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	98087	98087	98087
N_multimapping	1318037	1318037	1318037
N_noFeature	1105457	31652371	1243168
N_ambiguous	2228618	184763	932570
UnstrandedReadsAssigned:29773422 PositiveStrandReadsAssigned:1270363 NegativeStrandReadsAssigned:30931759
Dataset is classified negative stranded
MeadianReadLen=147 20thPercentileLength=115 echo kmer=111
SRR25257916 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25257916-trimmed-pair1.fastq
                             SRR25257916-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 34,523,557 reads, 32,609,251 reads pseudoaligned
[quant] estimated average fragment length: 153.546
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,462 rounds

 109283 SRR25257916.ke.tsv
  52636 SRR25257916.se.tsv
 161919 total
==> SRR25257916.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	11	0	0
ENSMUST00000196221.1	9	4	0	0
ENSMUST00000179664.1	11	6	0	0
ENSMUST00000178537.1	12	7	0	0
ENSMUST00000178862.1	14	9	0	0
ENSMUST00000179520.1	11	6	0	0
ENSMUST00000179883.1	16	11	0	0
ENSMUST00000195858.1	10	5	0	0
ENSMUST00000179932.1	12	7	0	0

==> SRR25257916.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25257916 completed mapping pipeline successfully
