Starting /dee2/code/volunteer_pipeline.sh SRR25257914
    current disk space = 666471088
    free memory = 195493996 
SRR25257914 SRAfilesize
cb46a594fb237bab454fbc5fc7ab14fc  SRR25257914.sra
SRR25257914.sra file validated
SRR25257914 is paired end
SRR25257914 is conventional basespace
SRR25257914 read1 length is 53-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257914_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	53-151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.671	37.0	37.0	37.0	37.0	37.0
2	36.4845	37.0	37.0	37.0	37.0	37.0
3	36.586	37.0	37.0	37.0	37.0	37.0
4	36.59	37.0	37.0	37.0	37.0	37.0
5	36.646	37.0	37.0	37.0	37.0	37.0
6	36.505	37.0	37.0	37.0	37.0	37.0
7	36.606	37.0	37.0	37.0	37.0	37.0
8	36.672	37.0	37.0	37.0	37.0	37.0
9	36.5635	37.0	37.0	37.0	37.0	37.0
10-14	36.6125	37.0	37.0	37.0	37.0	37.0
15-19	36.5914	37.0	37.0	37.0	37.0	37.0
20-24	36.564499999999995	37.0	37.0	37.0	37.0	37.0
25-29	36.45505	37.0	37.0	37.0	37.0	37.0
30-34	36.30114999999999	37.0	37.0	37.0	37.0	37.0
35-39	36.31395	37.0	37.0	37.0	37.0	37.0
40-44	36.4995	37.0	37.0	37.0	37.0	37.0
45-49	36.364549999999994	37.0	37.0	37.0	37.0	37.0
50-54	36.2156011627907	37.0	37.0	37.0	37.0	37.0
55-59	36.19870820756289	37.0	37.0	37.0	37.0	37.0
60-64	36.299978516622886	37.0	37.0	37.0	37.0	37.0
65-69	36.367952771998866	37.0	37.0	37.0	37.0	37.0
70-74	36.32276669705077	37.0	37.0	37.0	37.0	37.0
75-79	36.12795474589473	37.0	37.0	37.0	37.0	37.0
80-84	36.20621871678456	37.0	37.0	37.0	37.0	37.0
85-89	36.13372462937877	37.0	37.0	37.0	37.0	37.0
90-94	36.242721345457845	37.0	37.0	37.0	37.0	37.0
95-99	36.153543815288515	37.0	37.0	37.0	37.0	37.0
100-104	35.84015857753147	37.0	37.0	37.0	37.0	37.0
105-109	36.103877941847706	37.0	37.0	37.0	37.0	37.0
110-114	36.08027344789357	37.0	37.0	37.0	37.0	37.0
115-119	35.79938406670779	37.0	37.0	37.0	37.0	37.0
120-124	35.99921821060324	37.0	37.0	37.0	37.0	37.0
125-129	35.975395863549906	37.0	37.0	37.0	37.0	37.0
130-134	35.70432074919992	37.0	37.0	37.0	37.0	37.0
135-139	35.63356568968357	37.0	37.0	37.0	37.0	37.0
140-144	35.895531395193345	37.0	37.0	37.0	37.0	37.0
145-149	35.66929122230373	37.0	37.0	37.0	37.0	37.0
150-151	35.272457601057866	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	4.0
27	7.0
28	7.0
29	14.0
30	22.0
31	38.0
32	30.0
33	56.0
34	105.0
35	408.0
36	2991.0
37	318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.95	12.174999999999999	19.05	39.825
2	17.25	19.0	38.875	24.875
3	21.5	20.775	31.5	26.224999999999998
4	20.25	31.4	29.2	19.15
5	24.125	32.15	29.475	14.249999999999998
6	16.825000000000003	32.800000000000004	34.849999999999994	15.525
7	12.3	19.975	51.275000000000006	16.45
8	17.325	23.125	36.449999999999996	23.1
9	17.05	21.224999999999998	41.4	20.325
10-14	18.895	27.72	31.05	22.335
15-19	19.245	27.439999999999998	31.825	21.490000000000002
20-24	19.355	27.365000000000002	31.81	21.47
25-29	18.4248735793321	26.976418164522105	31.056926851249187	23.54178140489661
30-34	19.47999799227024	28.499723937158063	29.65416854891332	22.366109521658384
35-39	19.613550815558344	25.04391468005019	32.20577164366374	23.136762860727732
40-44	20.59	24.555	30.325000000000003	24.529999999999998
45-49	18.871897718726498	25.800952619704187	32.49435948859363	22.832790172975685
50-54	19.760088335675565	26.24472997390082	30.95763902830757	23.037542662116042
55-59	20.802295147976647	25.770082544795653	30.325145963358164	23.102476343869537
60-64	22.292929799067995	28.29082527433983	28.150523625795458	21.265721300796713
65-69	20.95018311342999	27.030552350373753	29.533938694627	22.485325841569257
70-74	20.540213666599477	27.837129610965533	28.90042330175368	22.722233420681313
75-79	21.763449608461304	26.192413302145834	31.185802908573173	20.85833418081969
80-84	20.98759101630602	27.027997128499642	29.704645677366425	22.279766177827913
85-89	21.802628833715836	25.902357604840393	29.23534320884623	23.05967035259754
90-94	23.898097319192026	25.971326546927465	28.833342216063528	21.29723391781698
95-99	20.34758620689655	26.20137931034483	30.240000000000002	23.21103448275862
100-104	20.48749494599434	26.29238144746722	29.931265523017387	23.288858083521053
105-109	22.13080295477749	26.22064740856405	28.232538586271094	23.416011050387365
110-114	21.18520398451875	26.19757629592031	28.323075946957683	24.29414377260326
115-119	22.822394347058026	27.632830547628757	28.26470987905966	21.280065226253566
120-124	21.857007506741493	26.06952846002478	30.894249690255815	21.179214342977918
125-129	21.583986074847694	26.948334520136086	29.464356357306748	22.00332304770947
130-134	20.333878323724292	26.495138112038553	30.703037604336973	22.46794595990018
135-139	20.352306089591817	27.5878397575528	29.15048773558102	22.909366417274363
140-144	20.035350384695363	25.077978789769183	31.597005614472863	23.28966521106259
145-149	20.871185651059864	28.08059631959003	28.662939669228976	22.385278360121127
150-151	22.635029659694037	26.94349047767718	28.785513581017796	21.63596628161099
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	5.5
23	19.0
24	18.5
25	25.0
26	53.0
27	64.0
28	69.5
29	83.0
30	86.0
31	75.0
32	67.5
33	79.5
34	89.5
35	92.5
36	113.5
37	139.5
38	131.5
39	147.0
40	179.5
41	180.0
42	198.5
43	224.5
44	217.5
45	168.5
46	164.0
47	173.0
48	164.5
49	144.5
50	99.5
51	82.0
52	76.0
53	87.0
54	82.5
55	52.5
56	39.0
57	33.5
58	37.0
59	42.5
60	45.5
61	31.0
62	18.5
63	25.0
64	16.5
65	8.5
66	8.5
67	22.5
68	27.5
69	11.0
70	5.0
71	12.5
72	10.5
73	2.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.135
30-34	0.385
35-39	0.375
40-44	0.0
45-49	0.27499999999999997
50-54	0.3750187509375469
55-59	0.5456274715923312
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.14724549378014726
80-84	0.0
85-89	0.23416766404745795
90-94	0.07455930127283378
95-99	0.31349686503134966
100-104	0.807837744929529
105-109	0.17386091127098321
110-114	0.18366054464851173
115-119	0.4127478178496516
120-124	0.04370947767174182
125-129	0.2997554626488917
130-134	0.7176420333190944
135-139	0.9009854528390426
140-144	0.26959767731231854
145-149	1.0373443983402488
150-151	0.7129572225666461
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52-53	1.0
54-55	3.0
56-57	1.0
58-59	2.0
60-61	2.0
62-63	1.0
64-65	1.0
66-67	2.0
68-69	10.0
70-71	8.0
72-73	9.0
74-75	13.0
76-77	18.0
78-79	10.0
80-81	18.0
82-83	18.0
84-85	22.0
86-87	33.0
88-89	32.0
90-91	42.0
92-93	44.0
94-95	51.0
96-97	43.0
98-99	68.0
100-101	57.0
102-103	59.0
104-105	49.0
106-107	86.0
108-109	68.0
110-111	71.0
112-113	79.0
114-115	76.0
116-117	96.0
118-119	88.0
120-121	67.0
122-123	93.0
124-125	79.0
126-127	88.0
128-129	70.0
130-131	82.0
132-133	76.0
134-135	74.0
136-137	120.0
138-139	74.0
140-141	58.0
142-143	86.0
144-145	78.0
146-147	77.0
148-149	67.0
150-151	1630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.65
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.760131434830235	28.65
2	14.84118291347207	13.55
3	7.667031763417305	10.5
4	4.271631982475356	7.8
5	3.0668127053669223	7.000000000000001
6	1.5881708652792992	4.35
7	1.4786418400876233	4.725
8	1.2048192771084338	4.3999999999999995
9	0.8214676889375685	3.375
>10	2.3001095290251916	15.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	35	0.8750000000000001	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	25	0.625	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	23	0.575	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	21	0.525	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	20	0.5	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	20	0.5	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	20	0.5	No Hit
CACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGC	19	0.475	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	19	0.475	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	18	0.44999999999999996	No Hit
CTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAA	18	0.44999999999999996	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	18	0.44999999999999996	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	16	0.4	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	16	0.4	No Hit
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	15	0.375	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	15	0.375	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	15	0.375	No Hit
GCACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATG	15	0.375	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	14	0.35000000000000003	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	14	0.35000000000000003	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	13	0.325	No Hit
CGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTG	13	0.325	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	13	0.325	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	12	0.3	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	12	0.3	No Hit
CAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAA	12	0.3	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	12	0.3	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	12	0.3	No Hit
CCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAGT	12	0.3	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	12	0.3	No Hit
GAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTT	12	0.3	No Hit
CTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCC	12	0.3	No Hit
ATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGC	12	0.3	No Hit
AGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCA	11	0.27499999999999997	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	10	0.25	No Hit
GTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAATC	10	0.25	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	10	0.25	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	10	0.25	No Hit
GTCCATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAG	10	0.25	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	10	0.25	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	10	0.25	No Hit
GTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGT	10	0.25	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	9	0.22499999999999998	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	9	0.22499999999999998	No Hit
GCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATA	9	0.22499999999999998	No Hit
ATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCA	9	0.22499999999999998	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	9	0.22499999999999998	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	9	0.22499999999999998	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	9	0.22499999999999998	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	9	0.22499999999999998	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	9	0.22499999999999998	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	9	0.22499999999999998	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	9	0.22499999999999998	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	9	0.22499999999999998	No Hit
ATTGGTTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTT	9	0.22499999999999998	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	9	0.22499999999999998	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	9	0.22499999999999998	No Hit
GTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGA	8	0.2	No Hit
ATTTTGAACATGCTGATGATTTAAAATCAAATTATTATTTTAATATTTGT	8	0.2	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	8	0.2	No Hit
TTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGC	8	0.2	No Hit
GTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCT	8	0.2	No Hit
CACCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCATC	8	0.2	No Hit
GAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGC	8	0.2	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	8	0.2	No Hit
GTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGG	8	0.2	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	8	0.2	No Hit
CTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCT	8	0.2	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	8	0.2	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	8	0.2	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	8	0.2	No Hit
CACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAGTGTTTT	8	0.2	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	8	0.2	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	8	0.2	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	8	0.2	No Hit
CTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCC	8	0.2	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	8	0.2	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	8	0.2	No Hit
TTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAG	8	0.2	No Hit
GCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCC	7	0.17500000000000002	No Hit
GTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG	7	0.17500000000000002	No Hit
CTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCTGCCTCC	7	0.17500000000000002	No Hit
TATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAG	7	0.17500000000000002	No Hit
GAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGC	7	0.17500000000000002	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	7	0.17500000000000002	No Hit
TGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTC	7	0.17500000000000002	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	7	0.17500000000000002	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	7	0.17500000000000002	No Hit
TGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTG	7	0.17500000000000002	No Hit
GCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCC	7	0.17500000000000002	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	7	0.17500000000000002	No Hit
CACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATTTGA	7	0.17500000000000002	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	7	0.17500000000000002	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	7	0.17500000000000002	No Hit
TCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATTCT	7	0.17500000000000002	No Hit
TGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCACATTTT	7	0.17500000000000002	No Hit
CGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCT	7	0.17500000000000002	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	7	0.17500000000000002	No Hit
ACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCA	7	0.17500000000000002	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	7	0.17500000000000002	No Hit
GTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTCGGC	7	0.17500000000000002	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	7	0.17500000000000002	No Hit
CTCATCCTTTCTGCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTG	7	0.17500000000000002	No Hit
AGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATT	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
CCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTC	7	0.17500000000000002	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	6	0.15	No Hit
TAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTT	6	0.15	No Hit
GAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACT	6	0.15	No Hit
TTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAG	6	0.15	No Hit
CTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCA	6	0.15	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	6	0.15	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	6	0.15	No Hit
CAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGG	6	0.15	No Hit
CTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGA	6	0.15	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	6	0.15	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	6	0.15	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	6	0.15	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	6	0.15	No Hit
GCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTG	6	0.15	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	6	0.15	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	6	0.15	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	6	0.15	No Hit
TAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCA	6	0.15	No Hit
TTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGC	6	0.15	No Hit
CTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCCAATT	6	0.15	No Hit
GTTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTT	6	0.15	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	6	0.15	No Hit
CCGCATTCTTTTTCTGCACCATTTTGTCCATCTTCTTGGCAATGCGAACC	6	0.15	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	6	0.15	No Hit
CGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGG	6	0.15	No Hit
CCACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATT	6	0.15	No Hit
CCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGAC	6	0.15	No Hit
CTGCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTGCTTCAGGGCT	6	0.15	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	6	0.15	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	5	0.125	No Hit
TTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAG	5	0.125	No Hit
GGACTGTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCA	5	0.125	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	5	0.125	No Hit
CGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACTGGC	5	0.125	No Hit
GCCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTC	5	0.125	No Hit
TTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATTA	5	0.125	No Hit
CCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCC	5	0.125	No Hit
TCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATA	5	0.125	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	5	0.125	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	5	0.125	No Hit
GTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCT	5	0.125	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	5	0.125	No Hit
CTGTATTCCTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGT	5	0.125	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	5	0.125	No Hit
GCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTG	5	0.125	No Hit
CCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATC	5	0.125	No Hit
TAGAGAGGTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAG	5	0.125	No Hit
ATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAGACT	5	0.125	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	5	0.125	No Hit
CTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAAATA	5	0.125	No Hit
GCTTTTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGA	5	0.125	No Hit
CAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCA	5	0.125	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	5	0.125	No Hit
TGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATT	5	0.125	No Hit
CTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGC	5	0.125	No Hit
TCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTCATCAGC	5	0.125	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	5	0.125	No Hit
CATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGA	5	0.125	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	5	0.125	No Hit
CTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATT	5	0.125	No Hit
CTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAA	5	0.125	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	5	0.125	No Hit
CTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGA	5	0.125	No Hit
GCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCA	5	0.125	No Hit
CATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACT	5	0.125	No Hit
TTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCAA	5	0.125	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	5	0.125	No Hit
TAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTT	5	0.125	No Hit
CAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTG	5	0.125	No Hit
TAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAG	5	0.125	No Hit
CATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTC	5	0.125	No Hit
GCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTAT	5	0.125	No Hit
TGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCC	5	0.125	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	5	0.125	No Hit
AAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAA	5	0.125	No Hit
CTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAAT	5	0.125	No Hit
CTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAG	5	0.125	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	5	0.125	No Hit
ATTTGGTTTTAAGCATAACATGTACAGAAAATGTAAACTTAAAATCGGCT	5	0.125	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	5	0.125	No Hit
GGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAA	5	0.125	No Hit
TTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCC	5	0.125	No Hit
CTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25257914 read2 length is 52-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257914_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	52-151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.402	37.0	37.0	37.0	37.0	37.0
2	36.20375	37.0	37.0	37.0	37.0	37.0
3	36.3655	37.0	37.0	37.0	37.0	37.0
4	36.2855	37.0	37.0	37.0	37.0	37.0
5	36.3785	37.0	37.0	37.0	37.0	37.0
6	35.9655	37.0	37.0	37.0	37.0	37.0
7	36.445	37.0	37.0	37.0	37.0	37.0
8	36.435	37.0	37.0	37.0	37.0	37.0
9	36.5225	37.0	37.0	37.0	37.0	37.0
10-14	36.2173	37.0	37.0	37.0	37.0	37.0
15-19	36.1378	37.0	37.0	37.0	37.0	37.0
20-24	36.16315	37.0	37.0	37.0	37.0	37.0
25-29	36.37665	37.0	37.0	37.0	37.0	37.0
30-34	36.31295	37.0	37.0	37.0	37.0	37.0
35-39	35.737300000000005	37.0	37.0	37.0	37.0	37.0
40-44	36.2847	37.0	37.0	37.0	37.0	37.0
45-49	36.10585	37.0	37.0	37.0	37.0	37.0
50-54	36.10109701540568	37.0	37.0	37.0	37.0	37.0
55-59	36.14565146951584	37.0	37.0	37.0	37.0	37.0
60-64	36.20662505580592	37.0	37.0	37.0	37.0	37.0
65-69	35.816002501926384	37.0	37.0	37.0	37.0	37.0
70-74	36.09149899957449	37.0	37.0	37.0	37.0	37.0
75-79	35.87173219531055	37.0	37.0	37.0	37.0	37.0
80-84	35.91603271332786	37.0	37.0	37.0	37.0	37.0
85-89	35.82003115746376	37.0	37.0	37.0	37.0	37.0
90-94	35.74305949481861	37.0	37.0	37.0	37.0	37.0
95-99	35.958939980293884	37.0	37.0	37.0	37.0	37.0
100-104	35.818339266393096	37.0	37.0	37.0	37.0	37.0
105-109	35.87296885865033	37.0	37.0	37.0	37.0	37.0
110-114	35.811941917098075	37.0	37.0	37.0	37.0	37.0
115-119	35.8286287640412	37.0	37.0	37.0	37.0	37.0
120-124	35.843007560019636	37.0	37.0	37.0	37.0	37.0
125-129	35.7037963645004	37.0	37.0	37.0	37.0	37.0
130-134	35.65470860278193	37.0	37.0	37.0	37.0	37.0
135-139	35.42193550566564	37.0	37.0	37.0	34.6	37.0
140-144	35.54253109344629	37.0	37.0	37.0	37.0	37.0
145-149	35.290168783263525	37.0	37.0	37.0	32.2	37.0
150-151	34.81935295962719	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	3.0
27	6.0
28	7.0
29	13.0
30	13.0
31	23.0
32	30.0
33	80.0
34	204.0
35	988.0
36	2435.0
37	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.4	15.275	18.9	34.425
2	23.471698113207548	24.60377358490566	32.930817610062896	18.9937106918239
3	23.799999999999997	26.025	28.125	22.05
4	27.075	33.5	20.1	19.325
5	24.875	35.8	21.175	18.15
6	22.261484098939928	39.04593639575972	21.47905098435134	17.213528520949016
7	19.425	19.025	40.1	21.45
8	21.175	24.75	28.599999999999998	25.474999999999998
9	22.85	25.224999999999998	28.249999999999996	23.674999999999997
10-14	24.16171583929111	28.733259490484343	24.040882086396135	23.064142583828414
15-19	24.165237566831433	29.340260264299406	25.30515484717038	21.18934732169878
20-24	22.810816254594894	30.917971700488444	25.43934739916411	20.831864645752553
25-29	22.672875669721098	29.547844374342798	25.47694156526964	22.302338390666463
30-34	23.398705923659527	29.227065255554997	25.595626222601194	21.77860259818428
35-39	23.880065159845245	29.724088780289147	25.687232742822236	20.708613317043373
40-44	22.845259571056324	29.77550611344959	25.711565443976745	21.667668871517336
45-49	22.264113004574472	29.72905041974564	26.466596290152317	21.540240285527574
50-54	23.213388280229168	30.6261935872952	25.1281535832747	21.032264549200924
55-59	22.034153691612254	29.422400803616274	26.78553490708187	21.7579105976896
60-64	22.793453157947585	30.143588713726277	26.011647755798773	21.05131037252736
65-69	22.630433682505945	29.88715145994636	26.041192247355905	21.441222610191794
70-74	23.30087289974267	28.987335385236392	27.216307583631867	20.495484131389073
75-79	23.420702365106994	29.968260468925976	26.159516740043003	20.45152042592403
80-84	22.921706902764193	31.034127760333558	26.51464456683997	19.529520770062284
85-89	23.115972367594726	30.39564580280511	26.75842579024492	19.729956039355244
90-94	22.712628865979383	31.051331615120276	26.5893470790378	19.64669243986254
95-99	22.306717363751584	31.65812530996859	26.91353942800463	19.121617898275197
100-104	22.455554916287902	32.23635003739716	25.717737759622576	19.590357286692363
105-109	22.98539751216874	32.16152875428159	24.41560002403702	20.43747370951265
110-114	21.343748015495017	31.8282847526513	26.906712389661525	19.921254842192162
115-119	23.203369107458226	31.40877598152425	26.70153511751121	18.686319793506318
120-124	22.62747100444963	33.62024947115034	24.604274564154935	19.148004960245093
125-129	23.249188889768142	30.774709187307113	26.50945635831289	19.466645564611852
130-134	22.383820998278832	31.46299483648881	26.970740103270224	19.182444061962133
135-139	21.930903928064364	31.225745385707526	26.37955513487932	20.46379555134879
140-144	22.164085391377146	32.35663457513604	26.87316868982838	18.606111343658434
145-149	21.74635188041286	32.55427690117452	26.48000949104283	19.219361727369794
150-151	20.829346092503986	31.89792663476874	28.00637958532695	19.26634768740032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	0.5
21	0.5
22	3.5
23	9.5
24	18.5
25	29.0
26	52.5
27	79.5
28	85.5
29	80.0
30	71.0
31	66.5
32	69.5
33	91.5
34	103.0
35	81.0
36	85.5
37	103.5
38	106.5
39	137.0
40	159.5
41	179.5
42	224.0
43	231.0
44	219.0
45	207.5
46	177.5
47	147.0
48	123.5
49	95.5
50	85.0
51	74.0
52	68.5
53	66.5
54	49.5
55	47.0
56	44.5
57	50.5
58	49.0
59	39.5
60	35.5
61	22.5
62	18.5
63	25.0
64	29.5
65	36.0
66	32.0
67	29.5
68	28.5
69	23.5
70	36.5
71	53.5
72	50.0
73	23.5
74	4.0
75	1.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.625
3	0.0
4	0.0
5	0.0
6	0.95
7	0.0
8	0.0
9	0.0
10-14	0.69
15-19	0.8699999999999999
20-24	0.705
25-29	0.145
30-34	0.315
35-39	1.78
40-44	0.22
45-49	0.5349999999999999
50-54	0.4950742611391709
55-59	0.29545795983774853
60-64	0.16040100250626568
65-69	0.8579169175195664
70-74	0.1209494532076803
75-79	0.7872416069886738
80-84	0.3334701415965524
85-89	0.5465049705928278
90-94	0.7936930698343366
95-99	0.21993731786440868
100-104	0.4011231448054553
105-109	0.17396520695860826
110-114	0.0951655881233346
115-119	0.15598507968802985
120-124	0.10929758088020985
125-129	0.2289594189167851
130-134	0.3772290809327846
135-139	0.4241281809613572
140-144	0.1254180602006689
145-149	0.47231078049356473
150-151	0.4129606099110546
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
52-53	2.0
54-55	3.0
56-57	2.0
58-59	2.0
60-61	1.0
62-63	1.0
64-65	0.0
66-67	2.0
68-69	10.0
70-71	8.0
72-73	10.0
74-75	13.0
76-77	18.0
78-79	11.0
80-81	18.0
82-83	18.0
84-85	21.0
86-87	32.0
88-89	33.0
90-91	41.0
92-93	45.0
94-95	48.0
96-97	43.0
98-99	72.0
100-101	55.0
102-103	59.0
104-105	51.0
106-107	87.0
108-109	70.0
110-111	72.0
112-113	79.0
114-115	77.0
116-117	97.0
118-119	80.0
120-121	69.0
122-123	90.0
124-125	80.0
126-127	94.0
128-129	73.0
130-131	79.0
132-133	79.0
134-135	77.0
136-137	115.0
138-139	75.0
140-141	62.0
142-143	110.0
144-145	86.0
146-147	74.0
148-149	65.0
150-151	1591.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.64999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.20726991492653	47.975
2	13.418406805877803	17.349999999999998
3	5.645784996133024	10.95
4	2.822892498066512	7.3
5	1.7014694508894046	5.5
6	0.8507347254447023	3.3000000000000003
7	0.42536736272235115	1.925
8	0.34802784222737815	1.7999999999999998
9	0.15467904098994587	0.8999999999999999
>10	0.42536736272235115	3.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	15	0.375	No Hit
CAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCA	12	0.3	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	11	0.27499999999999997	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	11	0.27499999999999997	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	11	0.27499999999999997	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	10	0.25	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	10	0.25	No Hit
CTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAG	10	0.25	No Hit
GGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCT	10	0.25	No Hit
CCAACGTCCGCGCAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAG	10	0.25	No Hit
CGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGCTGG	10	0.25	No Hit
GGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGC	9	0.22499999999999998	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	9	0.22499999999999998	No Hit
GGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATG	9	0.22499999999999998	No Hit
GCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAAC	9	0.22499999999999998	No Hit
GCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCTC	8	0.2	No Hit
CGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGG	8	0.2	No Hit
CAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTC	8	0.2	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	8	0.2	No Hit
TTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACT	8	0.2	No Hit
GGCATATTTAGAAATACTTGTGACTGGCTTCAAAATATAATTTCTTAAAT	8	0.2	No Hit
CACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATG	8	0.2	No Hit
CGGAGCCATGGAGGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAA	8	0.2	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	8	0.2	No Hit
CAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAA	7	0.17500000000000002	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	7	0.17500000000000002	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	7	0.17500000000000002	No Hit
CTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCT	7	0.17500000000000002	No Hit
CCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACT	7	0.17500000000000002	No Hit
CGGAAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAA	7	0.17500000000000002	No Hit
CTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATATTT	7	0.17500000000000002	No Hit
CGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCC	7	0.17500000000000002	No Hit
GCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAAT	7	0.17500000000000002	No Hit
ATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGC	7	0.17500000000000002	No Hit
CCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGA	7	0.17500000000000002	No Hit
ACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAA	6	0.15	No Hit
CGCAGCTTCTCTCGCTTCCCGTCCGCTGTGGCCGGGCGAGGGGAGGCCTT	6	0.15	No Hit
TTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGG	6	0.15	No Hit
GGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCAC	6	0.15	No Hit
GATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACA	6	0.15	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	6	0.15	No Hit
CCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAAC	6	0.15	No Hit
GGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTC	6	0.15	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	6	0.15	No Hit
CAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGGAAAAACCTG	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
TTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCT	6	0.15	No Hit
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	6	0.15	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	6	0.15	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	6	0.15	No Hit
GAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATT	6	0.15	No Hit
GGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCC	6	0.15	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	6	0.15	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	6	0.15	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	6	0.15	No Hit
GAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTA	6	0.15	No Hit
TGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAA	6	0.15	No Hit
TTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTT	5	0.125	No Hit
TTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCA	5	0.125	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	5	0.125	No Hit
TCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTAT	5	0.125	No Hit
GTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGA	5	0.125	No Hit
GTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAG	5	0.125	No Hit
AAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCAT	5	0.125	No Hit
GCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATGTT	5	0.125	No Hit
CCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAA	5	0.125	No Hit
GCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAAT	5	0.125	No Hit
TTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGT	5	0.125	No Hit
CGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCAT	5	0.125	No Hit
TGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAG	5	0.125	No Hit
GGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCC	5	0.125	No Hit
TCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATG	5	0.125	No Hit
CATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGT	5	0.125	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	5	0.125	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	5	0.125	No Hit
CAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATG	5	0.125	No Hit
ACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCC	5	0.125	No Hit
TTGTGACTGGCTTCAAAATATAATTTCTTAAATATCTTATAGCCGATTTT	5	0.125	No Hit
GTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATC	5	0.125	No Hit
GTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGG	5	0.125	No Hit
CTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAA	5	0.125	No Hit
CAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCA	5	0.125	No Hit
CAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGA	5	0.125	No Hit
GAATATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGT	5	0.125	No Hit
GAAATTTTCATCTTAAGTAAAGGAGCTTTTGATAATCAGTCTCCTGTACA	5	0.125	No Hit
GAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTG	5	0.125	No Hit
CTTAAGTTTATATATTTCAAATAACTGCACCTTAGTCCAACTTTAGACTG	5	0.125	No Hit
TGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAG	5	0.125	No Hit
CAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAA	5	0.125	No Hit
TAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAA	5	0.125	No Hit
TGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGAC	5	0.125	No Hit
CTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAAT	5	0.125	No Hit
ATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCC	5	0.125	No Hit
CTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGA	5	0.125	No Hit
TTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGATGGTATTAC	5	0.125	No Hit
GAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAG	5	0.125	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	5	0.125	No Hit
GTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCA	5	0.125	No Hit
CTTGCTGTTTTCCAACCAACCAAGAAGCACCTTTATAAACAGGTGCTAAT	5	0.125	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	5	0.125	No Hit
GGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Read 4059350 spots for SRR25257914.sra
Written 4059350 spots for SRR25257914.sra
Rejected 409 READS because READLEN < 1
Read 4059353 spots for SRR25257914.sra
Written 4059353 spots for SRR25257914.sra
SRR ids: ['SRR25257914.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_wv1lafl0
SRR25257914.sra spots: 32474803
blocks: [[1, 4059350], [4059351, 8118700], [8118701, 12178050], [12178051, 16237400], [16237401, 20296750], [20296751, 24356100], [24356101, 28415450], [28415451, 32474803]]
SRR25257914 file size 9850660
SRR25257914 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25257914 SRR25257914_1.fastq SRR25257914_2.fastq
Input file:	SRR25257914_1.fastq
Paired file:	SRR25257914_2.fastq
trimmed:	SRR25257914-trimmed-pair1.fastq, SRR25257914-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu Jun 18 11:56:01 2026 >> started

Thu Jun 18 11:58:40 2026 >> done (158.173s)
32474399 read pairs processed; of these:
       1 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
32474398 (100.00%) read pairs available; of these:
  267912 ( 0.82%) trimmed read pairs available after processing
32206486 (99.18%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       7	  0.00%
 19	       6	  0.00%
 20	       4	  0.00%
 21	      13	  0.00%
 22	       8	  0.00%
 23	      12	  0.00%
 24	      12	  0.00%
 25	      28	  0.00%
 26	      22	  0.00%
 27	      29	  0.00%
 28	      49	  0.00%
 29	      73	  0.00%
 30	      64	  0.00%
 31	     188	  0.00%
 32	     230	  0.00%
 33	     231	  0.00%
 34	     316	  0.00%
 35	     312	  0.00%
 36	     393	  0.00%
 37	     476	  0.00%
 38	     556	  0.00%
 39	     608	  0.00%
 40	     776	  0.00%
 41	     875	  0.00%
 42	    1039	  0.00%
 43	    1048	  0.00%
 44	    1222	  0.00%
 45	    1308	  0.00%
 46	    1571	  0.00%
 47	    1831	  0.01%
 48	    2128	  0.01%
 49	    2594	  0.01%
 50	    3045	  0.01%
 51	    3531	  0.01%
 52	    3955	  0.01%
 53	    4205	  0.01%
 54	    4486	  0.01%
 55	    4831	  0.01%
 56	    5060	  0.02%
 57	    5989	  0.02%
 58	    6800	  0.02%
 59	    8020	  0.02%
 60	    9163	  0.03%
 61	   10606	  0.03%
 62	   11463	  0.04%
 63	   13001	  0.04%
 64	   14185	  0.04%
 65	   14208	  0.04%
 66	   16152	  0.05%
 67	   17555	  0.05%
 68	   19351	  0.06%
 69	   21141	  0.07%
 70	   24097	  0.07%
 71	   26798	  0.08%
 72	   29922	  0.09%
 73	   33013	  0.10%
 74	   36613	  0.11%
 75	   40860	  0.13%
 76	   43481	  0.13%
 77	   48044	  0.15%
 78	   50192	  0.15%
 79	   57016	  0.18%
 80	   60514	  0.19%
 81	   65245	  0.20%
 82	   73121	  0.23%
 83	   80162	  0.25%
 84	   87035	  0.27%
 85	   95952	  0.30%
 86	  103407	  0.32%
 87	  109486	  0.34%
 88	  116443	  0.36%
 89	  126844	  0.39%
 90	  130897	  0.40%
 91	  141555	  0.44%
 92	  146708	  0.45%
 93	  162461	  0.50%
 94	  166540	  0.51%
 95	  181786	  0.56%
 96	  190912	  0.59%
 97	  199855	  0.62%
 98	  206056	  0.63%
 99	  211559	  0.65%
100	  224166	  0.69%
101	  227650	  0.70%
102	  234802	  0.72%
103	  245431	  0.76%
104	  257824	  0.79%
105	  261818	  0.81%
106	  272666	  0.84%
107	  281285	  0.87%
108	  288060	  0.89%
109	  294947	  0.91%
110	  290040	  0.89%
111	  291923	  0.90%
112	  300627	  0.93%
113	  300366	  0.92%
114	  305381	  0.94%
115	  312949	  0.96%
116	  314251	  0.97%
117	  326799	  1.01%
118	  323081	  0.99%
119	  324237	  1.00%
120	  323298	  1.00%
121	  320494	  0.99%
122	  323290	  1.00%
123	  318472	  0.98%
124	  324466	  1.00%
125	  322478	  0.99%
126	  316507	  0.97%
127	  323995	  1.00%
128	  330154	  1.02%
129	  314278	  0.97%
130	  322543	  0.99%
131	  315441	  0.97%
132	  308284	  0.95%
133	  315302	  0.97%
134	  299250	  0.92%
135	  306362	  0.94%
136	  303152	  0.93%
137	  308534	  0.95%
138	  291652	  0.90%
139	  297838	  0.92%
140	  298890	  0.92%
141	  290805	  0.90%
142	  297216	  0.92%
143	  286103	  0.88%
144	  287940	  0.89%
145	  290984	  0.90%
146	  372600	  1.15%
147	  444433	  1.37%
148	  349437	  1.08%
149	  260462	  0.80%
150	  354572	  1.09%
151	13715513	 42.23%
32474398 reads passed initial QC


criterion=sequence-density
sequence-density=0.64
sequence-density-rank=1
fanout-score=2.42
fanout-score-rank=33
prefix-density=0.70
prefix-fanout=2.2
sequence=TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=253.26
fanout-score-rank=1
prefix-density=0.33
prefix-fanout=6.8
sequence=TTCTTCATCTACCTCATTGTCAGCCTCCTGCTCCCCATTTTCCTCATTTTGAGCGTTCCCATTGGCAGGTGCATCTCTTCCATTCTCTGCCTCCTCCACAACTTCCTTCTTCTCCTTCAAGTCCTTGGTGGTGATCTCGGAGCTGGTATCCACTGCCGCGTCTGACATGGTGGGGCACGCCGGTGGTCCGATGCCGCGAGTGAGGGAATTAAAAGTCGAGTTCGAGGACTCTGGCGAGAGAGCTGCCGGAGTCCGCGCTGGAGGAGGCGGTGGGCGGCGAAGGTGGCTGCAGCGAGCGCGGAGCACGACCCAGGAACAATGCAAAGATGGCTTTTCG


criterion=sequence-density
sequence-density=0.54
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=34
prefix-density=0.54
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=25.45
fanout-score-rank=1
prefix-density=0.10
prefix-fanout=6.1
sequence=TGCTCCTGAGCTCGCGCGTTCCCTGCTTGCTGCCCTTCTGGCGCCCGCGCTGGTGGCACTACTGGTGTCGCCGGCCTCGGGTCGCGGAGGCCGGGACCACGGGGACTGGGACGTGGACAGGCGATTGCCTCCGCTGCCACCCCGCGAAGACGGGCCGCGCGTGGCCCGCTTCGTGACTCACGTCTCAGACTGGGGCTCGCTGGCCACTATCTCCACAATAAAGGAGGTGCGCGGCTGGCCCTTCGCGGACATCATCTCAATCAGTGACGGTCCTCCGGGAGAAGGCACGGGCGAGCCCTACATGTACCTGAGTCCACTGCAGCAAGCCGTGAGCGACCTGCAGGAAAATCCAGAGGCTACGCTGACTATGTCTTTAGCACAGACTGTCTACTGTAGGAATCATGGATTTGATCCCCAGAGTCCCCTGTGTGTTCATATAATGATGTCGGGAACTGTGACCAAGGTGAACAAGACAGAAGAGGACTATGCAAGGGATTCGCTGTTTGTTCGA
SRR25257914 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 18 11:59:03
                             Started mapping on |	Jun 18 11:59:03
                                    Finished on |	Jun 18 12:02:38
       Mapping speed, Million of reads per hour |	543.76

                          Number of input reads |	32474398
                      Average input read length |	265
                                    UNIQUE READS:
                   Uniquely mapped reads number |	31017570
                        Uniquely mapped reads % |	95.51%
                          Average mapped length |	265.27
                       Number of splices: Total |	32163674
            Number of splices: Annotated (sjdb) |	31946978
                       Number of splices: GT/AG |	31876046
                       Number of splices: GC/AG |	242127
                       Number of splices: AT/AC |	24844
               Number of splices: Non-canonical |	20657
                      Mismatch rate per base, % |	0.35%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.16
                        Insertion rate per base |	0.01%
                       Insertion average length |	2.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1364838
             % of reads mapped to multiple loci |	4.20%
        Number of reads mapped to too many loci |	73896
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.05%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	92041	92041	92041
N_multimapping	1364838	1364838	1364838
N_noFeature	1242770	29593530	1408023
N_ambiguous	2074585	245338	833469
UnstrandedReadsAssigned:27700215 PositiveStrandReadsAssigned:1178702 NegativeStrandReadsAssigned:28776078
Dataset is classified negative stranded
MeadianReadLen=142 20thPercentileLength=111 echo kmer=107
SRR25257914 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25257914-trimmed-pair1.fastq
                             SRR25257914-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 32,474,398 reads, 30,294,261 reads pseudoaligned
[quant] estimated average fragment length: 145.505
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,237 rounds

 109283 SRR25257914.ke.tsv
  52636 SRR25257914.se.tsv
 161919 total
==> SRR25257914.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25257914.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25257914 completed mapping pipeline successfully
