Starting /dee2/code/volunteer_pipeline.sh SRR25257912
    current disk space = 356139260
    free memory = 175851556 
SRR25257912 SRAfilesize
c835e96600a801fcbb438f949868ae0e  SRR25257912.sra
SRR25257912.sra file validated
SRR25257912 is paired end
SRR25257912 is conventional basespace
SRR25257912 read1 length is 51-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51-151
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6785	37.0	37.0	37.0	37.0	37.0
2	36.5845	37.0	37.0	37.0	37.0	37.0
3	36.6545	37.0	37.0	37.0	37.0	37.0
4	36.6595	37.0	37.0	37.0	37.0	37.0
5	36.646	37.0	37.0	37.0	37.0	37.0
6	36.6545	37.0	37.0	37.0	37.0	37.0
7	36.6465	37.0	37.0	37.0	37.0	37.0
8	36.735	37.0	37.0	37.0	37.0	37.0
9	36.6915	37.0	37.0	37.0	37.0	37.0
10-14	36.683400000000006	37.0	37.0	37.0	37.0	37.0
15-19	36.6413	37.0	37.0	37.0	37.0	37.0
20-24	36.6041	37.0	37.0	37.0	37.0	37.0
25-29	36.5221	37.0	37.0	37.0	37.0	37.0
30-34	36.387100000000004	37.0	37.0	37.0	37.0	37.0
35-39	36.425200000000004	37.0	37.0	37.0	37.0	37.0
40-44	36.5307	37.0	37.0	37.0	37.0	37.0
45-49	36.40985	37.0	37.0	37.0	37.0	37.0
50-54	36.30768795037678	37.0	37.0	37.0	37.0	37.0
55-59	36.26713089220633	37.0	37.0	37.0	37.0	37.0
60-64	36.37248519434812	37.0	37.0	37.0	37.0	37.0
65-69	36.41060757563014	37.0	37.0	37.0	37.0	37.0
70-74	36.40958009083611	37.0	37.0	37.0	37.0	37.0
75-79	36.29724838772445	37.0	37.0	37.0	37.0	37.0
80-84	36.36147642668483	37.0	37.0	37.0	37.0	37.0
85-89	36.33625168659011	37.0	37.0	37.0	37.0	37.0
90-94	36.32966330785736	37.0	37.0	37.0	37.0	37.0
95-99	36.177447194812785	37.0	37.0	37.0	37.0	37.0
100-104	36.09253626813656	37.0	37.0	37.0	37.0	37.0
105-109	36.295430328002986	37.0	37.0	37.0	37.0	37.0
110-114	36.18073558713303	37.0	37.0	37.0	37.0	37.0
115-119	36.067510878990404	37.0	37.0	37.0	37.0	37.0
120-124	36.133337959881274	37.0	37.0	37.0	37.0	37.0
125-129	36.098962600104464	37.0	37.0	37.0	37.0	37.0
130-134	36.010373352666385	37.0	37.0	37.0	37.0	37.0
135-139	35.87881974459274	37.0	37.0	37.0	37.0	37.0
140-144	36.10239892769938	37.0	37.0	37.0	37.0	37.0
145-149	35.80730017389594	37.0	37.0	37.0	37.0	37.0
150-151	35.41446731431166	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	1.0
27	3.0
28	2.0
29	13.0
30	14.0
31	23.0
32	37.0
33	45.0
34	72.0
35	355.0
36	3002.0
37	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.325	13.65	18.425	41.6
2	17.849999999999998	18.175	41.199999999999996	22.775000000000002
3	20.4	21.0	32.25	26.35
4	20.75	31.4	28.575	19.275000000000002
5	22.025	34.1	31.0	12.875
6	16.225	30.725	34.825	18.224999999999998
7	11.225	19.975	51.975	16.825000000000003
8	17.525	22.225	37.025000000000006	23.225
9	17.299999999999997	22.775000000000002	39.725	20.200000000000003
10-14	19.405	28.095	30.714999999999996	21.785
15-19	18.485	27.955000000000002	31.585	21.975
20-24	19.38	27.279999999999998	31.35	21.990000000000002
25-29	17.808150595774507	27.265445078602184	31.460899168919592	23.465505156703713
30-34	19.533132530120483	28.11244979919679	30.2359437751004	22.11847389558233
35-39	19.22189912764464	25.56903639827534	31.79083525518901	23.418229218891007
40-44	19.515	25.2	31.47	23.815
45-49	19.18928410174083	26.819846485727183	31.55571163397381	22.43515777855817
50-54	18.95851101188983	28.570711884814127	30.206190738975568	22.264586364320476
55-59	20.66032512959887	26.679752378076397	29.51331219487644	23.146610297448287
60-64	21.71013039117352	28.26980942828485	28.254764292878637	21.76529588766299
65-69	20.382005529027396	27.650163357627545	29.52500628298568	22.44282483035939
70-74	20.62105528906842	27.053774299419338	30.477152234284272	21.848018177227974
75-79	21.03545014027034	26.335118592195865	31.62458556490691	21.00484570262688
80-84	20.02257220540707	26.737803314010154	31.06243266813728	22.177191812445493
85-89	20.119667013527575	25.900104058272632	31.004162330905306	22.976066597294484
90-94	22.77201383346635	25.921787709497206	30.587922319765898	20.71827613727055
95-99	20.47914720589043	26.40254959063685	30.30386284960712	22.8144403538656
100-104	21.19449403904855	26.159073892760464	30.00057593733802	22.645856130852962
105-109	21.705938927626832	26.442654484251022	28.96729983169031	22.884106756431834
110-114	20.87267159989793	26.798928298035214	28.278897677979074	24.049502424087777
115-119	22.01061080125153	27.003128826010066	29.30213576384165	21.68412460889675
120-124	21.38917114240093	25.700391929162432	31.267237625199595	21.643199303237047
125-129	21.42350995984568	26.95850720415715	29.635461774663412	21.982521061333752
130-134	19.317985113380647	26.683399688419595	31.253245629219318	22.74536956898044
135-139	18.95680366167636	28.683131496138074	29.465051969104607	22.895012873080958
140-144	20.111146062703156	26.96864842193562	30.324001258257315	22.59620425710391
145-149	20.60747663551402	29.088785046728972	28.925233644859816	21.378504672897197
150-151	20.399875039050297	26.085598250546703	31.084036238675417	22.430490471727584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.5
20	1.5
21	0.5
22	5.5
23	11.0
24	14.0
25	30.0
26	53.5
27	75.0
28	82.0
29	82.5
30	83.5
31	64.0
32	66.0
33	83.5
34	94.5
35	102.5
36	115.0
37	143.5
38	152.0
39	157.5
40	180.0
41	210.0
42	229.5
43	215.5
44	208.5
45	191.5
46	177.0
47	176.5
48	153.5
49	119.0
50	98.0
51	93.0
52	74.5
53	68.0
54	70.5
55	49.5
56	29.0
57	23.0
58	34.0
59	44.0
60	34.5
61	20.0
62	16.0
63	18.0
64	9.0
65	2.5
66	7.5
67	21.5
68	26.0
69	13.5
70	5.5
71	7.0
72	8.5
73	6.0
74	1.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.13
30-34	0.4
35-39	0.27
40-44	0.0
45-49	0.335
50-54	0.31007751937984496
55-59	0.550578107012363
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.2391614085080399
80-84	0.0
85-89	0.12990387113535984
90-94	0.10098862549165515
95-99	0.3886152162014231
100-104	0.7374799908529613
105-109	0.11407985589912939
110-114	0.16558400203795695
115-119	0.3929539295392954
120-124	0.0870195794053662
125-129	0.24348099277411248
130-134	0.6107526881720431
135-139	0.7758539123852777
140-144	0.2405857740585774
145-149	0.730604198074916
150-151	0.5591798695246971
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	3.0
55-59	5.0
60-64	9.0
65-69	14.0
70-74	25.0
75-79	28.0
80-84	48.0
85-89	67.0
90-94	100.0
95-99	136.0
100-104	157.0
105-109	191.0
110-114	188.0
115-119	184.0
120-124	206.0
125-129	220.0
130-134	229.0
135-139	203.0
140-144	202.0
145-149	162.0
150-152	1623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.67538126361656	27.85
2	17.59259259259259	16.150000000000002
3	7.570806100217865	10.424999999999999
4	4.466230936819173	8.200000000000001
5	2.505446623093682	5.75
6	1.6884531590413945	4.65
7	1.3071895424836601	4.2
8	1.0348583877995643	3.8
9	0.7080610021786492	2.9250000000000003
>10	2.450980392156863	16.05
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	35	0.8750000000000001	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	26	0.65	No Hit
CAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGCT	24	0.6	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	24	0.6	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	22	0.5499999999999999	No Hit
AGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCA	22	0.5499999999999999	No Hit
CACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGC	21	0.525	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	21	0.525	No Hit
AGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAA	18	0.44999999999999996	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	17	0.42500000000000004	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	16	0.4	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	16	0.4	No Hit
CACCATTTTGTCCATCTTCTTGGCAATGCGAACCACCTCGTCCTCCATGG	15	0.375	No Hit
GTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGA	14	0.35000000000000003	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	14	0.35000000000000003	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	14	0.35000000000000003	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	13	0.325	No Hit
ATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCA	13	0.325	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	13	0.325	No Hit
CATTATTTTATGGTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAG	13	0.325	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	13	0.325	No Hit
TAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTT	12	0.3	No Hit
ATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCA	12	0.3	No Hit
CTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAA	12	0.3	No Hit
GGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCC	12	0.3	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	12	0.3	No Hit
CTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTC	11	0.27499999999999997	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	11	0.27499999999999997	No Hit
TGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTC	11	0.27499999999999997	No Hit
CTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAAATA	11	0.27499999999999997	No Hit
CTCATCCTTTCTGCTGCTTACATTGCTGCTGGAACTACTTTCTTCTCTTG	11	0.27499999999999997	No Hit
CAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTG	11	0.27499999999999997	No Hit
CTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGC	11	0.27499999999999997	No Hit
CAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGTGAAC	11	0.27499999999999997	No Hit
TTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGAG	10	0.25	No Hit
GCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGTGAACAA	10	0.25	No Hit
CATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATGA	10	0.25	No Hit
GTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGG	10	0.25	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	10	0.25	No Hit
TTCAGAAATAACCATAATTTTTGCAACATATTCAACTGTGTGGTAACAGT	10	0.25	No Hit
CTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTC	10	0.25	No Hit
CTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAAT	10	0.25	No Hit
CTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAG	10	0.25	No Hit
CCACACAGCACATTTTTCCTTAAATTTGGATTCTTTGCATCTTTAAGATT	10	0.25	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	10	0.25	No Hit
CTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAA	9	0.22499999999999998	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	9	0.22499999999999998	No Hit
CTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCA	9	0.22499999999999998	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	9	0.22499999999999998	No Hit
CTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGAGA	9	0.22499999999999998	No Hit
CTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAAT	9	0.22499999999999998	No Hit
CTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCCCCT	9	0.22499999999999998	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	9	0.22499999999999998	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	9	0.22499999999999998	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	9	0.22499999999999998	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	9	0.22499999999999998	No Hit
CTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGA	9	0.22499999999999998	No Hit
AATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTC	9	0.22499999999999998	No Hit
GCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCC	8	0.2	No Hit
CTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGTGAACAAC	8	0.2	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	8	0.2	No Hit
GTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACAGAATC	8	0.2	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	8	0.2	No Hit
GTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCT	8	0.2	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	8	0.2	No Hit
GTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCT	8	0.2	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	8	0.2	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	8	0.2	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	8	0.2	No Hit
AATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCAT	8	0.2	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	8	0.2	No Hit
CCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCATCAG	8	0.2	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	8	0.2	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	8	0.2	No Hit
CTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATTTCC	8	0.2	No Hit
ATTGGTTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTT	8	0.2	No Hit
CTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTC	8	0.2	No Hit
CCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATCT	7	0.17500000000000002	No Hit
GTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTG	7	0.17500000000000002	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	7	0.17500000000000002	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	7	0.17500000000000002	No Hit
CAAGATATTACTTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGA	7	0.17500000000000002	No Hit
CCTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAA	7	0.17500000000000002	No Hit
CTCCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACA	7	0.17500000000000002	No Hit
CCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTTTT	7	0.17500000000000002	No Hit
GGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTT	7	0.17500000000000002	No Hit
TTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCCAAT	7	0.17500000000000002	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	7	0.17500000000000002	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	7	0.17500000000000002	No Hit
CTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCT	7	0.17500000000000002	No Hit
TAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCA	7	0.17500000000000002	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	7	0.17500000000000002	No Hit
TTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCAA	7	0.17500000000000002	No Hit
GTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTTAAA	7	0.17500000000000002	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	7	0.17500000000000002	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	7	0.17500000000000002	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	7	0.17500000000000002	No Hit
CAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACAT	7	0.17500000000000002	No Hit
ACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGATG	7	0.17500000000000002	No Hit
CTTCTTGGCAATGCGAACCACCTCGTCCTCCATGGCTCCGGAAGGCCTCC	7	0.17500000000000002	No Hit
CTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGTA	7	0.17500000000000002	No Hit
GAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTC	6	0.15	No Hit
AGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGTGAACA	6	0.15	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	6	0.15	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	6	0.15	No Hit
GGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAG	6	0.15	No Hit
GGTTCATCAGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTT	6	0.15	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	6	0.15	No Hit
CATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGCAA	6	0.15	No Hit
TGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTG	6	0.15	No Hit
AGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTTTCTGCACCAT	6	0.15	No Hit
CGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTG	6	0.15	No Hit
GGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGC	6	0.15	No Hit
ACGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACTGG	6	0.15	No Hit
GCTGGAACTACTTTCTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTG	6	0.15	No Hit
CACCGATTTCCACATTCATTACATACAACAAATGTTGTCATTGGTTCATC	6	0.15	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	6	0.15	No Hit
CGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCT	6	0.15	No Hit
CTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTCATCAGCT	6	0.15	No Hit
GCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAAT	6	0.15	No Hit
GAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAA	6	0.15	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	6	0.15	No Hit
CCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTCG	6	0.15	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	6	0.15	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	6	0.15	No Hit
AGAGAGGTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGA	6	0.15	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATC	6	0.15	No Hit
CGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGG	6	0.15	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	6	0.15	No Hit
CCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACT	6	0.15	No Hit
TTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAG	6	0.15	No Hit
CCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATG	6	0.15	No Hit
TTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAG	5	0.125	No Hit
GGACTGTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAGCA	5	0.125	No Hit
CCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTG	5	0.125	No Hit
GAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGC	5	0.125	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	5	0.125	No Hit
GCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATA	5	0.125	No Hit
ATTTTGAACATGCTGATGATTTAAAATCAAATTATTATTTTAATATTTGT	5	0.125	No Hit
CTGCTGTCATTCTAGCAAATAGATCAGGAGGAATATTCCCACACAGCACA	5	0.125	No Hit
CAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAA	5	0.125	No Hit
CTAGAGAGGTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACA	5	0.125	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	5	0.125	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	5	0.125	No Hit
TTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGC	5	0.125	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	5	0.125	No Hit
TCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTTTTCTGC	5	0.125	No Hit
GTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGC	5	0.125	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	5	0.125	No Hit
CACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATG	5	0.125	No Hit
CTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGC	5	0.125	No Hit
ACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGACACTTAA	5	0.125	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	5	0.125	No Hit
GTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGT	5	0.125	No Hit
TCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCGAAGAGCTGC	5	0.125	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	5	0.125	No Hit
GTCCATCTAATAATTTTTTCCAGGATTTGATGAGAGACTTGGCTAGAGAG	5	0.125	No Hit
TTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATT	5	0.125	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	5	0.125	No Hit
GAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGCCGCATTCTTT	5	0.125	No Hit
GTCTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGA	5	0.125	No Hit
GTGACTTCTTCATCTGTGCTCTGCTTGCGAAGGGCATTGACAGACATTCC	5	0.125	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	5	0.125	No Hit
GGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCT	5	0.125	No Hit
CTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCC	5	0.125	No Hit
CATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTC	5	0.125	No Hit
GATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCT	5	0.125	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	5	0.125	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	5	0.125	No Hit
CTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTCTTT	5	0.125	No Hit
CCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGTGA	5	0.125	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	5	0.125	No Hit
GGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTG	5	0.125	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	5	0.125	No Hit
GACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTCCC	5	0.125	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	5	0.125	No Hit
TGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAAT	5	0.125	No Hit
ATTTGATGTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25257912 read2 length is 51-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25257912_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51-151
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5255	37.0	37.0	37.0	37.0	37.0
2	36.2305	37.0	37.0	37.0	37.0	37.0
3	36.459	37.0	37.0	37.0	37.0	37.0
4	36.3035	37.0	37.0	37.0	37.0	37.0
5	36.11525	37.0	37.0	37.0	37.0	37.0
6	35.918	37.0	37.0	37.0	37.0	37.0
7	36.4255	37.0	37.0	37.0	37.0	37.0
8	36.39	37.0	37.0	37.0	37.0	37.0
9	36.519	37.0	37.0	37.0	37.0	37.0
10-14	36.18085	37.0	37.0	37.0	37.0	37.0
15-19	36.11785	37.0	37.0	37.0	37.0	37.0
20-24	36.194199999999995	37.0	37.0	37.0	37.0	37.0
25-29	36.33985	37.0	37.0	37.0	37.0	37.0
30-34	36.26219999999999	37.0	37.0	37.0	37.0	37.0
35-39	35.64925	37.0	37.0	37.0	37.0	37.0
40-44	36.3279	37.0	37.0	37.0	37.0	37.0
45-49	36.11695	37.0	37.0	37.0	37.0	37.0
50-54	36.07858606825293	37.0	37.0	37.0	37.0	37.0
55-59	36.11063534087798	37.0	37.0	37.0	37.0	37.0
60-64	36.19494798889969	37.0	37.0	37.0	37.0	37.0
65-69	35.79893967493497	37.0	37.0	37.0	37.0	37.0
70-74	36.03300216431402	37.0	37.0	37.0	37.0	37.0
75-79	35.86166876328267	37.0	37.0	37.0	37.0	37.0
80-84	35.94469096772112	37.0	37.0	37.0	37.0	37.0
85-89	35.87337313093724	37.0	37.0	37.0	37.0	37.0
90-94	35.707573290309284	37.0	37.0	37.0	37.0	37.0
95-99	35.88374813195641	37.0	37.0	37.0	37.0	37.0
100-104	35.82835841614523	37.0	37.0	37.0	37.0	37.0
105-109	35.81454326900357	37.0	37.0	37.0	37.0	37.0
110-114	35.807406973991206	37.0	37.0	37.0	37.0	37.0
115-119	35.78233662174624	37.0	37.0	37.0	37.0	37.0
120-124	35.774474831171176	37.0	37.0	37.0	37.0	37.0
125-129	35.62165906869252	37.0	37.0	37.0	37.0	37.0
130-134	35.65569372177085	37.0	37.0	37.0	37.0	37.0
135-139	35.42783762909896	37.0	37.0	37.0	34.6	37.0
140-144	35.43955328249965	37.0	37.0	37.0	34.6	37.0
145-149	35.19425206149564	37.0	37.0	37.0	32.2	37.0
150-151	34.70397919695071	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	2.0
26	2.0
27	2.0
28	7.0
29	8.0
30	9.0
31	22.0
32	40.0
33	86.0
34	219.0
35	1033.0
36	2396.0
37	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.275000000000002	15.825	20.575	33.324999999999996
2	24.035087719298247	24.035087719298247	33.33333333333333	18.596491228070175
3	24.25	26.650000000000002	28.1	21.0
4	25.825	35.525	19.275000000000002	19.375
5	27.29559748427673	35.672955974842765	20.0251572327044	17.0062893081761
6	21.742424242424242	39.09090909090909	21.11111111111111	18.055555555555554
7	19.575	18.6	40.9	20.925
8	21.5	25.324999999999996	28.825	24.349999999999998
9	22.400000000000002	25.7	29.125	22.775000000000002
10-14	24.122122021260516	28.480024182578468	25.00881656506625	22.389037231094765
15-19	23.745144529082378	29.914745497654238	25.03657367704182	21.303536296221562
20-24	22.479605196897975	31.256924161546983	25.435592708228423	20.82787793332662
25-29	22.817311067649566	30.58021162429166	26.247429918258863	20.35504738979991
30-34	23.796147672552166	29.34390048154093	26.09851524879615	20.761436597110755
35-39	23.0749630535596	29.5724405034908	26.784895275951687	20.56770116699791
40-44	22.72408612919379	29.969954932398597	26.214321482223333	21.091637456184277
45-49	22.928079609991457	30.446801025280195	26.235110820726742	20.39000854400161
50-54	22.273001205303338	31.257533145841705	25.813579750903976	20.655885897950984
55-59	21.847095414869067	29.657870974214912	26.91883214608207	21.576201464833954
60-64	22.95254833040422	30.253577705247302	25.83981923173487	20.95405473261361
65-69	22.40320698229056	29.969046531689248	27.096970619576798	20.530775866443395
70-74	22.23740133576199	29.89779396883222	27.964986844768262	19.899817850637522
75-79	23.12153561896941	30.034900431122974	26.95031820981318	19.893245740094436
80-84	22.647634752138515	31.57270947129754	26.89889724827373	18.88075852829022
85-89	22.47425913343438	30.727016150107143	27.037056394710707	19.761668321747766
90-94	22.30698973835491	31.5854510288508	26.422392951163165	19.685166281631115
95-99	22.42667252554103	30.92936394595188	27.463473580138416	19.18048994836867
100-104	22.914993690489847	31.616381782723412	26.276241826316394	19.192382700470347
105-109	22.41618009992175	32.28194787214832	25.425871305604048	19.87600072232589
110-114	21.917283635435282	32.110033188664794	27.32320653561399	18.649476640285933
115-119	23.34714955493647	31.881497587823603	26.46599171026704	18.30536114697289
120-124	22.493638676844785	33.12250090876045	25.583424209378407	18.800436205016357
125-129	22.263436192881382	32.4520558756215	26.36729539894247	18.917212532554654
130-134	22.42942242942243	31.373564706898037	26.366226366226364	19.830786497453165
135-139	21.002748554639368	31.43777840962942	27.959435124632737	19.600037911098475
140-144	22.904442581726737	31.95725062866723	26.78122380553227	18.357082984073763
145-149	21.28157156220767	32.179607109448085	27.128157156220766	19.41066417212348
150-151	23.446768654386513	30.533874492663127	28.254761161411178	17.76459569153918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	3.0
22	9.0
23	10.5
24	17.5
25	32.5
26	47.5
27	68.5
28	75.5
29	71.0
30	61.5
31	62.5
32	89.0
33	118.0
34	118.5
35	96.5
36	99.5
37	117.5
38	124.5
39	128.0
40	141.0
41	181.5
42	231.5
43	238.0
44	236.5
45	216.5
46	187.5
47	176.5
48	136.5
49	87.5
50	59.5
51	60.5
52	58.5
53	54.5
54	56.0
55	54.5
56	38.5
57	34.0
58	36.5
59	36.5
60	41.5
61	26.5
62	14.5
63	15.0
64	16.5
65	28.0
66	29.5
67	36.0
68	34.5
69	19.0
70	30.5
71	41.0
72	36.0
73	18.0
74	3.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.25
3	0.0
4	0.0
5	0.625
6	1.0
7	0.0
8	0.0
9	0.0
10-14	0.755
15-19	0.885
20-24	0.7100000000000001
25-29	0.295
30-34	0.32
35-39	1.8849999999999998
40-44	0.15
45-49	0.515
50-54	0.41510377594398595
55-59	0.22523649832323941
60-64	0.12537612838515547
65-69	0.9449610454888162
70-74	0.18181818181818182
75-79	0.748815648718863
80-84	0.313350798787692
85-89	0.3800895553472873
90-94	0.7782930859853937
95-99	0.19187544542514115
100-104	0.3315801509261377
105-109	0.1322512774271115
110-114	0.1783893985728848
115-119	0.10181916915557969
120-124	0.07990701728897283
125-129	0.2754604124035889
130-134	0.3269942345753377
135-139	0.40589012648669054
140-144	0.05236150382238977
145-149	0.5234384087472373
150-151	0.1869741352446245
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	3.0
55-59	5.0
60-64	9.0
65-69	14.0
70-74	30.0
75-79	27.0
80-84	51.0
85-89	71.0
90-94	99.0
95-99	126.0
100-104	162.0
105-109	187.0
110-114	190.0
115-119	188.0
120-124	204.0
125-129	220.0
130-134	219.0
135-139	205.0
140-144	214.0
145-149	158.0
150-152	1618.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22758100979652	49.25
2	14.619442351168049	19.400000000000002
3	5.501130369253956	10.95
4	2.3360964581763377	6.2
5	1.3941220798794274	4.625
6	0.5275056518462697	2.1
7	0.640542577241899	2.9749999999999996
8	0.48982667671439334	2.6
9	0.11303692539562923	0.675
>10	0.15071590052750566	1.225
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGA	14	0.35000000000000003	No Hit
CCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATT	14	0.35000000000000003	No Hit
CAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGT	11	0.27499999999999997	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	10	0.25	No Hit
CTTAAATATCTTATAGCCGATTTTAAGTTTACATTTTCTGTACATGTTAT	9	0.22499999999999998	No Hit
CGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGA	9	0.22499999999999998	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	9	0.22499999999999998	No Hit
GGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCAC	8	0.2	No Hit
CTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAA	8	0.2	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	8	0.2	No Hit
TGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATCT	8	0.2	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	8	0.2	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	8	0.2	No Hit
GACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCA	8	0.2	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	8	0.2	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	8	0.2	No Hit
CCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACT	8	0.2	No Hit
CTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAG	8	0.2	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	8	0.2	No Hit
GGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACA	8	0.2	No Hit
ATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGC	7	0.17500000000000002	No Hit
CTCGTAGTGCTGATGAACCAATGACAACATTTGTTGTATGTAATGAATGT	7	0.17500000000000002	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	7	0.17500000000000002	No Hit
TGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTG	7	0.17500000000000002	No Hit
CCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAAT	7	0.17500000000000002	No Hit
GGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATG	7	0.17500000000000002	No Hit
CAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTT	7	0.17500000000000002	No Hit
GCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAG	7	0.17500000000000002	No Hit
GTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAA	7	0.17500000000000002	No Hit
CTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCCAGGGCTA	7	0.17500000000000002	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	7	0.17500000000000002	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	7	0.17500000000000002	No Hit
AGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCG	7	0.17500000000000002	No Hit
CTCGTTTCCTTGCAAGTGAAATTTGTAAACAACATACATCTCATGGGTTG	7	0.17500000000000002	No Hit
CGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAAT	7	0.17500000000000002	No Hit
AAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAG	7	0.17500000000000002	No Hit
GAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGGCAACTCGTTT	7	0.17500000000000002	No Hit
CTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCAATGACAACATTT	6	0.15	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	6	0.15	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	6	0.15	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	6	0.15	No Hit
CTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAA	6	0.15	No Hit
GTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGC	6	0.15	No Hit
GGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGTA	6	0.15	No Hit
CAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCA	6	0.15	No Hit
CAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAA	6	0.15	No Hit
GCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAAT	6	0.15	No Hit
GGACCATTAAGAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGG	6	0.15	No Hit
CCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGC	6	0.15	No Hit
GGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGA	6	0.15	No Hit
TGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGA	6	0.15	No Hit
CTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCG	5	0.125	No Hit
GCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTG	5	0.125	No Hit
CGCGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGT	5	0.125	No Hit
GGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGC	5	0.125	No Hit
ACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAA	5	0.125	No Hit
TCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTAT	5	0.125	No Hit
TATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCC	5	0.125	No Hit
TAAAATGTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACC	5	0.125	No Hit
CCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAA	5	0.125	No Hit
TGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAG	5	0.125	No Hit
CTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAG	5	0.125	No Hit
CCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTC	5	0.125	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	5	0.125	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	5	0.125	No Hit
CCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAAT	5	0.125	No Hit
TTGTGACTGGCTTCAAAATATAATTTCTTAAATATCTTATAGCCGATTTT	5	0.125	No Hit
TTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTC	5	0.125	No Hit
GGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGCATTGCCAAGAA	5	0.125	No Hit
CTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCAATGACAA	5	0.125	No Hit
TAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGAC	5	0.125	No Hit
CACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGA	5	0.125	No Hit
GGGAGGCAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGA	5	0.125	No Hit
TTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTC	5	0.125	No Hit
TTTAGAAATACTTGTGACTGGCTTCAAAATATAATTTCTTAAATATCTTA	5	0.125	No Hit
TCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGC	5	0.125	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	5	0.125	No Hit
CACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATG	5	0.125	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	5	0.125	No Hit
GGACGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAA	5	0.125	No Hit
TTCCAACCTAGGTAATATGAAAAGAAATCAAACATTACTTAAGTTTATAT	5	0.125	No Hit
CTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAA	5	0.125	No Hit
GGCCGGGCGAGGGGAGGCCTTCCGGAGCCATGGAGGACGAGGTGGTTCGC	5	0.125	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	5	0.125	No Hit
CACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGC	5	0.125	No Hit
CAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTAGTCCAAC	5	0.125	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	5	0.125	No Hit
GAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCCA	10	0.009541876	129.5974	7
TGTCCAA	10	0.009541876	129.5974	8
>>END_MODULE
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Read 3561433 spots for SRR25257912.sra
Written 3561433 spots for SRR25257912.sra
Rejected 287 READS because READLEN < 1
Read 3561439 spots for SRR25257912.sra
Written 3561439 spots for SRR25257912.sra
SRR ids: ['SRR25257912.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_yogn2g8l
SRR25257912.sra spots: 28491470
blocks: [[1, 3561433], [3561434, 7122866], [7122867, 10684299], [10684300, 14245732], [14245733, 17807165], [17807166, 21368598], [21368599, 24930031], [24930032, 28491470]]
SRR25257912 file size 8623296
SRR25257912 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25257912 SRR25257912_1.fastq SRR25257912_2.fastq
Input file:	SRR25257912_1.fastq
Paired file:	SRR25257912_2.fastq
trimmed:	SRR25257912-trimmed-pair1.fastq, SRR25257912-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu Jun 18 11:41:13 2026 >> started

Thu Jun 18 11:42:05 2026 >> done (52.517s)
28491187 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
28491187 (100.00%) read pairs available; of these:
  233582 ( 0.82%) trimmed read pairs available after processing
28257605 (99.18%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       6	  0.00%
 19	       8	  0.00%
 20	       8	  0.00%
 21	      14	  0.00%
 22	      18	  0.00%
 23	      15	  0.00%
 24	      16	  0.00%
 25	      18	  0.00%
 26	      36	  0.00%
 27	      45	  0.00%
 28	      75	  0.00%
 29	      85	  0.00%
 30	     133	  0.00%
 31	     195	  0.00%
 32	     233	  0.00%
 33	     292	  0.00%
 34	     352	  0.00%
 35	     398	  0.00%
 36	     487	  0.00%
 37	     577	  0.00%
 38	     697	  0.00%
 39	     766	  0.00%
 40	     945	  0.00%
 41	    1117	  0.00%
 42	    1223	  0.00%
 43	    1373	  0.00%
 44	    1357	  0.00%
 45	    1525	  0.01%
 46	    1720	  0.01%
 47	    1988	  0.01%
 48	    2355	  0.01%
 49	    2884	  0.01%
 50	    3293	  0.01%
 51	    3766	  0.01%
 52	    4281	  0.02%
 53	    4394	  0.02%
 54	    4884	  0.02%
 55	    4910	  0.02%
 56	    5624	  0.02%
 57	    5896	  0.02%
 58	    6985	  0.02%
 59	    7957	  0.03%
 60	    9243	  0.03%
 61	   10513	  0.04%
 62	   11482	  0.04%
 63	   12352	  0.04%
 64	   13902	  0.05%
 65	   13424	  0.05%
 66	   15320	  0.05%
 67	   16240	  0.06%
 68	   18066	  0.06%
 69	   19939	  0.07%
 70	   21914	  0.08%
 71	   24691	  0.09%
 72	   27505	  0.10%
 73	   30258	  0.11%
 74	   33341	  0.12%
 75	   37211	  0.13%
 76	   40124	  0.14%
 77	   43753	  0.15%
 78	   45246	  0.16%
 79	   50959	  0.18%
 80	   54278	  0.19%
 81	   58869	  0.21%
 82	   65473	  0.23%
 83	   71432	  0.25%
 84	   77115	  0.27%
 85	   84821	  0.30%
 86	   92309	  0.32%
 87	   97828	  0.34%
 88	  103366	  0.36%
 89	  112433	  0.39%
 90	  116503	  0.41%
 91	  125497	  0.44%
 92	  130044	  0.46%
 93	  142763	  0.50%
 94	  146963	  0.52%
 95	  160612	  0.56%
 96	  167973	  0.59%
 97	  177406	  0.62%
 98	  181432	  0.64%
 99	  187931	  0.66%
100	  200170	  0.70%
101	  202256	  0.71%
102	  208303	  0.73%
103	  217382	  0.76%
104	  228332	  0.80%
105	  231568	  0.81%
106	  242080	  0.85%
107	  252109	  0.88%
108	  256895	  0.90%
109	  263443	  0.92%
110	  256532	  0.90%
111	  260318	  0.91%
112	  264968	  0.93%
113	  266165	  0.93%
114	  270862	  0.95%
115	  275914	  0.97%
116	  278195	  0.98%
117	  289718	  1.02%
118	  285909	  1.00%
119	  288341	  1.01%
120	  284629	  1.00%
121	  285524	  1.00%
122	  287178	  1.01%
123	  281864	  0.99%
124	  286936	  1.01%
125	  283216	  0.99%
126	  281298	  0.99%
127	  287440	  1.01%
128	  294409	  1.03%
129	  279113	  0.98%
130	  285498	  1.00%
131	  276757	  0.97%
132	  271588	  0.95%
133	  277639	  0.97%
134	  264409	  0.93%
135	  272101	  0.96%
136	  265198	  0.93%
137	  272289	  0.96%
138	  258433	  0.91%
139	  263091	  0.92%
140	  264269	  0.93%
141	  259954	  0.91%
142	  262094	  0.92%
143	  252605	  0.89%
144	  254106	  0.89%
145	  255810	  0.90%
146	  327113	  1.15%
147	  391103	  1.37%
148	  305138	  1.07%
149	  229739	  0.81%
150	  313265	  1.10%
151	11854439	 41.61%
28491187 reads passed initial QC


criterion=sequence-density
sequence-density=0.75
sequence-density-rank=1
fanout-score=2.06
fanout-score-rank=42
prefix-density=0.76
prefix-fanout=2.0
sequence=AAAAGCAGGAGGAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=167.66
fanout-score-rank=1
prefix-density=0.89
prefix-fanout=1.4
sequence=CACAGAACCCCAACAAGGCAGACGGGAAGACAGGAAAGCAGAATGGAAGGCTGACCCAGGGACAGCAACATCCCAAGCAAGCCACCTGCAGGTGCTTCTGAAATCTCTGTGCTATGACAATGGCCATTCCATCGCTCTAAAGGCCTGATCATTCTGTGCTTCTAATCATCCAAAATAACTCCTTGGTACCACAGGAAGCTGGGTAGCTCCCTCATGGTAGGAGATGGCCATAGATCCCCCACACACCAAGAAAGGGCTCAGAAAATGCTCCTGCCCTCTGGCTCACCACCCCCCTCCAGCAGGTGGGTGAAAAGGGCAGCCGGGGAGCTGATGCCCAAGGTATCCTCATCCTTCGAGCAGAAAGGGGACACGGAAGTGGCTGTCAGCACCTCTTACATGACAGAAATGCTACAGTAAGGGAGAGGAGATGGGGGAAGGGCAAAGGGGGTTCCTACTTATTAAGTCAAATAGATCTATGTGTTCATTACCCTAGACCCACAAGTAGGTGACT


criterion=sequence-density
sequence-density=0.72
sequence-density-rank=1
fanout-score=3.23
fanout-score-rank=25
prefix-density=0.85
prefix-fanout=2.7
sequence=TTCCTCCTGCTTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=140.63
fanout-score-rank=1
prefix-density=0.25
prefix-fanout=10.6
sequence=AAAGAAAAAAAAATGTATTTTGAAGCCATTTTCAGAGAAAAATGCCATTCATTATAACCAAGGAAGTTTTCTTTCAGCCATTCCCGGTGGCTTTTCCTCAGTGGTGCCCACAGAAGGGCAACATAGACCAGCTGTAGATCGCCCATTGGCTCTGGAGGAAGAGAGGTGCAGGTGCTTTGTGCCCGACGGCCACTGTGCCTGAGGGGTGCTGTCCTCACACAGCTGCCTCAGGAGTCTGTCAGAGGGGCCTAGAGTCGGGAGGCCTGGGCAGGGCGTGGGTCCCGGATACAGAGTGCTTGAATGAATCCTTCAGTCGAGGACAGCAATGGCCACACACGGAGCTTCCCACTCACGTCCACCCGTACAGCTGATCCATCACAAGGCAAAACTGGGTCCTCTATAAAACTCCATAAACATCATACATCCTAAAGTCATAGTTACCCAGGACAAAATAAGACAAAAAGATTTTCCTGTATTCATTTTCCACCCAAGTAGACTGTATATTTATTTC
SRR25257912 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Jun 18 11:42:21
                             Started mapping on |	Jun 18 11:42:21
                                    Finished on |	Jun 18 11:45:08
       Mapping speed, Million of reads per hour |	614.18

                          Number of input reads |	28491187
                      Average input read length |	264
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27296936
                        Uniquely mapped reads % |	95.81%
                          Average mapped length |	264.82
                       Number of splices: Total |	29576951
            Number of splices: Annotated (sjdb) |	29384634
                       Number of splices: GT/AG |	29191100
                       Number of splices: GC/AG |	346143
                       Number of splices: AT/AC |	22195
               Number of splices: Non-canonical |	17513
                      Mismatch rate per base, % |	0.34%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.14
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.97
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1115526
             % of reads mapped to multiple loci |	3.92%
        Number of reads mapped to too many loci |	64275
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.05%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	78764	78764	78764
N_multimapping	1115526	1115526	1115526
N_noFeature	941031	25980259	1075016
N_ambiguous	1997608	205148	842835
UnstrandedReadsAssigned:24358297 PositiveStrandReadsAssigned:1111529 NegativeStrandReadsAssigned:25379085
Dataset is classified negative stranded
MeadianReadLen=141 20thPercentileLength=111 echo kmer=107
SRR25257912 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25257912-trimmed-pair1.fastq
                             SRR25257912-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,491,187 reads, 26,819,102 reads pseudoaligned
[quant] estimated average fragment length: 147.179
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,451 rounds

 109283 SRR25257912.ke.tsv
  52636 SRR25257912.se.tsv
 161919 total
==> SRR25257912.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	2	0	0
ENSMUST00000178537.1	12	3	0	0
ENSMUST00000178862.1	14	5	0	0
ENSMUST00000179520.1	11	2	0	0
ENSMUST00000179883.1	16	7	0	0
ENSMUST00000195858.1	10	1	0	0
ENSMUST00000179932.1	12	3	0	0

==> SRR25257912.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25257912 completed mapping pipeline successfully
