Starting /dee2/code/volunteer_pipeline.sh SRR25454238
    current disk space = 1249693664
    free memory = 187604656 
SRR25454238 SRAfilesize
309d23f4ef590855cab484f979dd14e8  SRR25454238.sra
SRR25454238.sra file validated
SRR25454238 is single end
SRR25454238 is conventional basespace
SRR25454238 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25454238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28625	32.0	32.0	32.0	32.0	32.0
2	31.45375	32.0	32.0	32.0	32.0	32.0
3	35.22375	37.0	32.0	37.0	32.0	37.0
4	35.91	37.0	37.0	37.0	32.0	37.0
5	35.98875	37.0	37.0	37.0	32.0	37.0
6	39.32875	41.0	41.0	41.0	37.0	41.0
7	39.52275	41.0	41.0	41.0	37.0	41.0
8	39.67	41.0	41.0	41.0	37.0	41.0
9	39.332	41.0	41.0	41.0	37.0	41.0
10	39.06825	41.0	41.0	41.0	37.0	41.0
11	39.49675	41.0	41.0	41.0	37.0	41.0
12	39.41375	41.0	41.0	41.0	37.0	41.0
13	39.46875	41.0	41.0	41.0	37.0	41.0
14	38.8825	41.0	37.0	41.0	32.0	41.0
15	39.43775	41.0	41.0	41.0	37.0	41.0
16	39.51825	41.0	41.0	41.0	37.0	41.0
17	38.8525	41.0	37.0	41.0	37.0	41.0
18	39.25375	41.0	41.0	41.0	37.0	41.0
19	38.578	41.0	37.0	41.0	32.0	41.0
20	38.2975	41.0	37.0	41.0	32.0	41.0
21	38.4515	41.0	37.0	41.0	32.0	41.0
22	38.494	41.0	37.0	41.0	32.0	41.0
23	37.69775	41.0	37.0	41.0	32.0	41.0
24	37.561	41.0	37.0	41.0	27.0	41.0
25	37.8495	41.0	37.0	41.0	32.0	41.0
26	37.1305	41.0	37.0	41.0	27.0	41.0
27	36.5855	41.0	37.0	41.0	27.0	41.0
28	37.25275	41.0	37.0	41.0	27.0	41.0
29	37.10075	41.0	37.0	41.0	27.0	41.0
30	37.322	41.0	37.0	41.0	27.0	41.0
31	36.9335	41.0	37.0	41.0	27.0	41.0
32	36.8935	41.0	37.0	41.0	27.0	41.0
33	36.82875	41.0	37.0	41.0	27.0	41.0
34	36.79725	41.0	37.0	41.0	27.0	41.0
35	37.3975	41.0	37.0	41.0	27.0	41.0
36	36.7995	41.0	37.0	41.0	27.0	41.0
37	37.18325	41.0	37.0	41.0	27.0	41.0
38	37.37	41.0	37.0	41.0	27.0	41.0
39	37.25925	41.0	37.0	41.0	27.0	41.0
40	37.109	41.0	37.0	41.0	27.0	41.0
41	36.925	41.0	37.0	41.0	27.0	41.0
42	36.90175	41.0	37.0	41.0	27.0	41.0
43	36.92875	41.0	37.0	41.0	27.0	41.0
44	36.9645	41.0	37.0	41.0	27.0	41.0
45	37.043	41.0	37.0	41.0	27.0	41.0
46	36.91375	41.0	37.0	41.0	27.0	41.0
47	36.87275	41.0	37.0	41.0	27.0	41.0
48	37.27775	41.0	37.0	41.0	27.0	41.0
49	37.1475	41.0	37.0	41.0	27.0	41.0
50	37.04475	41.0	37.0	41.0	27.0	41.0
51	36.30725	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	5.0
27	16.0
28	24.0
29	30.0
30	60.0
31	79.0
32	122.0
33	140.0
34	174.0
35	245.0
36	338.0
37	548.0
38	826.0
39	996.0
40	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.396396396396398	21.32132132132132	19.21921921921922	38.06306306306306
2	14.57185778668002	21.532298447671508	47.39609414121182	16.499749624436653
3	16.675	25.525	33.75	24.05
4	19.7	30.25	27.0	23.05
5	21.475	33.575	24.4	20.549999999999997
6	21.825	34.1	23.599999999999998	20.474999999999998
7	21.5	28.449999999999996	28.675	21.375
8	19.8	26.625	28.050000000000004	25.525
9	18.4	24.425	32.125	25.05
10	21.55	28.4	28.775000000000002	21.275
11	22.45	28.749999999999996	24.2	24.6
12	22.825	26.700000000000003	26.950000000000003	23.525
13	20.875	25.624999999999996	29.975	23.525
14	22.650000000000002	27.6	25.8	23.95
15	23.275000000000002	27.075	27.800000000000004	21.85
16	22.650000000000002	27.700000000000003	26.900000000000002	22.75
17	22.35	27.35	25.900000000000002	24.4
18	21.8	28.799999999999997	25.8	23.599999999999998
19	21.15	28.799999999999997	25.525	24.525
20	19.975	30.175	28.925	20.925
21	22.675	28.825	26.525	21.975
22	22.400000000000002	27.925	27.1	22.575
23	21.224999999999998	29.275000000000002	27.85	21.65
24	22.475	27.05	28.449999999999996	22.025
25	20.625	28.725	27.125	23.525
26	23.025000000000002	28.775000000000002	25.074999999999996	23.125
27	22.675	28.525	25.575	23.225
28	22.225	27.224999999999998	27.725	22.825
29	21.075	27.0	28.925	23.0
30	21.05	28.349999999999998	27.500000000000004	23.1
31	22.75	27.700000000000003	27.325	22.225
32	23.75	28.1	27.675	20.474999999999998
33	23.325000000000003	27.075	27.625	21.975
34	21.2	28.299999999999997	27.425	23.075000000000003
35	21.95	27.425	27.650000000000002	22.975
36	24.075	27.224999999999998	26.6	22.1
37	22.400000000000002	28.675	26.875	22.05
38	22.175	28.675	25.674999999999997	23.474999999999998
39	23.849999999999998	27.325	26.400000000000002	22.425
40	22.675	24.8	29.525000000000002	23.0
41	22.925	26.125	28.675	22.275
42	22.425	28.475	26.375	22.725
43	22.675	26.974999999999998	26.375	23.974999999999998
44	23.225	27.325	27.625	21.825
45	21.9	27.6	27.6	22.900000000000002
46	24.5	27.675	27.200000000000003	20.625
47	22.725	29.375	26.700000000000003	21.2
48	22.975	28.999999999999996	24.075	23.95
49	22.725	27.425	25.374999999999996	24.474999999999998
50	21.85	29.275000000000002	26.35	22.525000000000002
51	22.5	28.249999999999996	26.875	22.375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	7.5
4	14.0
5	12.5
6	11.0
7	10.0
8	9.0
9	8.5
10	8.0
11	9.0
12	10.0
13	8.0
14	6.0
15	10.0
16	14.0
17	14.0
18	14.0
19	15.5
20	17.0
21	28.0
22	39.0
23	45.5
24	52.0
25	68.0
26	88.5
27	93.0
28	90.0
29	87.0
30	119.0
31	151.0
32	157.5
33	164.0
34	174.0
35	184.0
36	176.0
37	168.0
38	205.0
39	242.0
40	295.5
41	349.0
42	313.5
43	278.0
44	253.5
45	229.0
46	208.0
47	187.0
48	216.0
49	245.0
50	227.5
51	210.0
52	199.5
53	189.0
54	196.5
55	204.0
56	176.0
57	148.0
58	137.5
59	127.0
60	128.5
61	130.0
62	96.0
63	62.0
64	70.5
65	79.0
66	74.5
67	70.0
68	46.0
69	22.0
70	22.0
71	22.0
72	17.5
73	13.0
74	12.5
75	22.0
76	32.0
77	26.5
78	21.0
79	12.0
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.14999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87029930928625	47.475
2	15.195702225633154	19.8
3	6.062931696085956	11.85
4	2.532617037605526	6.6000000000000005
5	1.4581734458940905	4.75
6	0.7674597083653107	3.0
7	0.34535686876438987	1.575
8	0.2686108979278588	1.4000000000000001
9	0.15349194167306215	0.8999999999999999
>10	0.34535686876438987	2.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTA	19	0.475	No Hit
CTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAGCGACCCT	15	0.375	No Hit
CTTCAATTTCTCTATGCAAGCTATTGACCAGGTTATAAACTCAGCTGCCAA	11	0.27499999999999997	No Hit
CTTTATTCCTTATCAAAACAATTAAACAGCAAATGTAGATAATATACTTCT	11	0.27499999999999997	No Hit
CTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTT	10	0.25	No Hit
CTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTTTGACT	10	0.25	No Hit
CTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGA	10	0.25	No Hit
ATGAATGGGTGCCAGAAAGCAGAGTACTCAAATACGTGGACACCAATTTGC	10	0.25	No Hit
CTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTATGGCC	10	0.25	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTC	9	0.22499999999999998	No Hit
CTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGAC	9	0.22499999999999998	No Hit
CTGCAGGAATCACCTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGGC	9	0.22499999999999998	No Hit
CTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAA	9	0.22499999999999998	No Hit
TTTCAAACAAATGCCAACTGGAAGACAATGTCCAAAAACTCTGAAGCTTCC	8	0.2	No Hit
CTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTA	8	0.2	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCT	8	0.2	No Hit
CTCTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTT	8	0.2	No Hit
CTGTGAATTTATGACCTTCAATTTCTCTATGCAAGCTATTGACCAGGTTAT	8	0.2	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCC	8	0.2	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCC	8	0.2	No Hit
GTTGTAGGAGGCTCCTGCAGGAATCACCTCCACTGCAGGCACCTGGGAAGG	7	0.17500000000000002	No Hit
CAAACAAATGCCAACTGGAAGACAATGTCCAAAAACTCTGAAGCTTCCAGA	7	0.17500000000000002	No Hit
ATTTAATGTTTTCATTAAATTGTTTCAATACAATTTCAAACAAATGCCAAC	7	0.17500000000000002	No Hit
GTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTA	7	0.17500000000000002	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAG	7	0.17500000000000002	No Hit
CTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGC	7	0.17500000000000002	No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.17500000000000002	No Hit
TTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAATA	7	0.17500000000000002	No Hit
ATTAAATTGTTTCAATACAATTTCAAACAAATGCCAACTGGAAGACAATGT	7	0.17500000000000002	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	6	0.15	No Hit
CTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATAT	6	0.15	No Hit
TGAATTTATGACCTTCAATTTCTCTATGCAAGCTATTGACCAGGTTATAAA	6	0.15	No Hit
CATTAAATTGTTTCAATACAATTTCAAACAAATGCCAACTGGAAGACAATG	6	0.15	No Hit
CTCCACTGCAGGCACCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCT	6	0.15	No Hit
GACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAG	6	0.15	No Hit
CCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	6	0.15	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	6	0.15	No Hit
CTGGGCTTCAGGTTGGCCAGGCTCACTCTCGGCAAGGACCGCAGCAGGTCC	6	0.15	No Hit
GTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAATAAATC	6	0.15	No Hit
CAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGG	6	0.15	No Hit
GTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAGTG	6	0.15	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	6	0.15	No Hit
CTTCTTCTGGACCAAGGTCCTCTTCTTTCTTGGTTCTTTTCTCTTGAATCC	6	0.15	No Hit
AATTGTTTCAATACAATTTCAAACAAATGCCAACTGGAAGACAATGTCCAA	6	0.15	No Hit
GGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAG	6	0.15	No Hit
CCTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGCCTTTCTTCT	6	0.15	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAAT	6	0.15	No Hit
TTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTTTGACTG	6	0.15	No Hit
TGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	6	0.15	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGA	5	0.125	No Hit
CTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAAAATGT	5	0.125	No Hit
TTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATCTTGGCATATTT	5	0.125	No Hit
ATTCAAGAGAAAAGAACCAAGAAAGAAGAGGACCTTGGTCCAGAAGAAGTC	5	0.125	No Hit
ATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCC	5	0.125	No Hit
CTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTT	5	0.125	No Hit
GGTCGCTCAATGATGTCCTGGGGCCCAGGTTTGGCCTTTGGTGTGGGAGGG	5	0.125	No Hit
CTTCTATTGGGAGACCAGGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAG	5	0.125	No Hit
GTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAA	5	0.125	No Hit
GTTTTCATTAAATTGTTTCAATACAATTTCAAACAAATGCCAACTGGAAGA	5	0.125	No Hit
CAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATACTGTAGTC	5	0.125	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	5	0.125	No Hit
CTGGGAAGGCTTGATGTGGAGGCGTTGTGGCCGCCTCACGCCTTTCTTCTT	5	0.125	No Hit
GACCATGGAGCATGCTGCTTACTGACTTGCTCCTCATGGCTTGCTCAGCCT	5	0.125	No Hit
CTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGG	5	0.125	No Hit
CCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCC	5	0.125	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCA	5	0.125	No Hit
CTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGC	5	0.125	No Hit
CTGGGGCCCAGGTTTGGCCTTTGGTGTGGGAGGGCTAAGGAGTCGGGCCTG	5	0.125	No Hit
ATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAA	5	0.125	No Hit
TTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACA	5	0.125	No Hit
CAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGA	5	0.125	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGG	5	0.125	No Hit
CAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTT	5	0.125	No Hit
AGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATAGCTTGCATAGAGA	5	0.125	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAAT	5	0.125	No Hit
CCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	5	0.125	No Hit
GTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTCGGCAAGGACCGCAG	5	0.125	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAG	5	0.125	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGA	5	0.125	No Hit
CAAATGCCAACTGGAAGACAATGTCCAAAAACTCTGAAGCTTCCAGACAAG	5	0.125	No Hit
ATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGAC	5	0.125	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATT	5	0.125	No Hit
CTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGACATCATTGAG	5	0.125	No Hit
CGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAA	5	0.125	No Hit
CTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCTT	5	0.125	No Hit
CTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTTTGACTGAGCT	5	0.125	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.075	0.0	0.0	0.0	0.0
36	0.15	0.0	0.0	0.0	0.0
37	0.225	0.0	0.0	0.0	0.0
38	0.5	0.0	0.0	0.0	0.0
39	0.65	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
Read 2371464 spots for SRR25454238.sra
Written 2371464 spots for SRR25454238.sra
SRR ids: ['SRR25454238.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_k_uk_rgd
SRR25454238.sra spots: 18971712
blocks: [[1, 2371464], [2371465, 4742928], [4742929, 7114392], [7114393, 9485856], [9485857, 11857320], [11857321, 14228784], [14228785, 16600248], [16600249, 18971712]]
SRR25454238 file size 2701780
SRR25454238 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25454238 SRR25454238_1.fastq
Input file:	SRR25454238_1.fastq
trimmed:	SRR25454238-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Wed May 27 18:31:58 2026 >> started

Wed May 27 18:32:04 2026 >> done (5.894s)
18971712 reads processed; of these:
    1804 ( 0.01%) short reads filtered out after trimming by size control
   31459 ( 0.17%) empty reads filtered out after trimming by size control
18938449 (99.82%) reads available; of these:
     655 ( 0.00%) trimmed reads available after processing
18937794 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     272	  0.00%
 19	     136	  0.00%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	      15	  0.00%
 50	     232	  0.00%
 51	18937794	100.00%
18938449 reads passed initial QC


criterion=sequence-density
sequence-density=0.69
sequence-density-rank=1
fanout-score=71.50
fanout-score-rank=1
prefix-density=1.30
prefix-fanout=38.1
sequence=AGATCGGAAGAGCACACG


criterion=fanout-score
sequence-density=0.69
sequence-density-rank=1
fanout-score=71.50
fanout-score-rank=1
prefix-density=1.30
prefix-fanout=38.1
sequence=AGATCGGAAGAGCACACG
                                 Started job on |	May 27 18:32:10
                             Started mapping on |	May 27 18:32:10
                                    Finished on |	May 27 18:32:46
       Mapping speed, Million of reads per hour |	1893.84

                          Number of input reads |	18938449
                      Average input read length |	50
                                    UNIQUE READS:
                   Uniquely mapped reads number |	13092504
                        Uniquely mapped reads % |	69.13%
                          Average mapped length |	50.18
                       Number of splices: Total |	1779386
            Number of splices: Annotated (sjdb) |	1730154
                       Number of splices: GT/AG |	1756198
                       Number of splices: GC/AG |	10783
                       Number of splices: AT/AC |	2121
               Number of splices: Non-canonical |	10284
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.42
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.14
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3738782
             % of reads mapped to multiple loci |	19.74%
        Number of reads mapped to too many loci |	255553
             % of reads mapped to too many loci |	1.35%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.72%
                     % of reads unmapped: other |	1.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2107163	2107163	2107163
N_multimapping	3738782	3738782	3738782
N_noFeature	2993841	7963160	7755126
N_ambiguous	546805	95127	95515
UnstrandedReadsAssigned:9551858 PositiveStrandReadsAssigned:5034217 NegativeStrandReadsAssigned:5241863
Dataset is classified unstranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR25454238 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25454238-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 18,938,449 reads, 13,181,460 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,141 rounds

 109283 SRR25454238.ke.tsv
  52636 SRR25454238.se.tsv
 161919 total
==> SRR25454238.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25454238.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25454238 completed mapping pipeline successfully
