Starting /dee2/code/volunteer_pipeline.sh SRR25558505
    current disk space = 460546546688
    free memory = 358402776 
SRR25558505 SRAfilesize
b85f1e0f8da2a935c662b3cd4123c7a9  SRR25558505.sra
SRR25558505.sra file validated
SRR25558505 is paired end
SRR25558505 is conventional basespace
SRR25558505 read1 length is 99 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558505_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	99
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5955	31.0	31.0	34.0	30.0	34.0
2	30.03375	31.0	30.0	34.0	25.0	34.0
3	34.59675	37.0	35.0	37.0	30.0	37.0
4	35.0205	37.0	35.0	37.0	32.0	37.0
5	35.24525	37.0	35.0	37.0	32.0	37.0
6	35.36975	37.0	35.0	37.0	33.0	37.0
7	35.23975	37.0	35.0	37.0	32.0	37.0
8	36.663	39.0	37.0	39.0	32.0	39.0
9	36.864	39.0	37.0	39.0	33.0	39.0
10-11	36.817	39.0	37.0	39.0	32.0	39.0
12-13	37.405249999999995	39.5	37.0	40.0	32.5	40.0
14-15	38.143375	40.0	37.5	41.0	33.0	41.0
16-17	38.198625	40.0	38.0	41.0	33.0	41.0
18-19	38.220625	40.0	38.0	41.0	33.5	41.0
20-21	37.941874999999996	40.0	37.0	41.0	32.5	41.0
22-23	37.808	40.0	37.0	41.0	32.5	41.0
24-25	37.75725	40.0	37.0	41.0	32.0	41.0
26-27	37.649	39.5	37.0	41.0	32.0	41.0
28-29	37.5995	40.0	37.0	41.0	32.0	41.0
30-31	37.79625	40.0	37.0	41.0	32.5	41.0
32-33	37.814499999999995	40.0	37.0	41.0	33.0	41.0
34-35	37.847125	40.0	37.0	41.0	33.0	41.0
36-37	37.614625000000004	40.0	37.0	41.0	32.0	41.0
38-39	37.36925	39.5	37.0	41.0	31.0	41.0
40-41	37.3155	39.0	36.5	40.0	31.0	41.0
42-43	37.259249999999994	39.0	36.0	40.0	31.0	41.0
44-45	37.299375	39.0	36.5	40.0	31.0	41.0
46-47	37.251	39.0	36.0	40.0	31.0	41.0
48-49	37.234875	39.0	36.0	40.0	31.5	41.0
50-51	37.145125	39.0	36.0	40.0	31.0	41.0
52-53	36.89775	39.0	36.0	40.0	30.5	41.0
54-55	36.67725	39.0	35.5	40.0	30.0	41.0
56-57	36.702375	38.5	35.5	40.0	31.0	41.0
58-59	36.445125000000004	38.0	35.0	40.0	30.0	41.0
60-61	36.086124999999996	38.0	34.5	40.0	29.5	41.0
62-63	35.720749999999995	37.0	34.0	39.5	29.0	41.0
64-65	35.242999999999995	37.0	34.0	39.0	29.0	40.0
66-67	34.70625	36.5	34.0	39.0	28.0	40.0
68-69	34.161	36.0	33.0	37.5	27.0	39.0
70-71	33.651	35.0	32.0	37.0	27.5	39.0
72-73	33.261250000000004	35.0	32.0	36.5	26.5	38.5
74-75	31.928625000000004	34.0	30.5	35.0	26.0	36.5
76-77	31.920499999999997	34.0	30.5	35.0	26.0	36.0
78-79	32.5285	34.0	32.0	35.0	26.5	36.0
80-81	32.40975	34.0	32.0	35.0	27.0	36.0
82-83	32.248374999999996	34.0	32.0	35.0	26.0	36.0
84-85	32.04	34.0	32.0	35.0	26.0	35.5
86-87	31.802625	34.0	32.0	35.0	26.0	35.0
88-89	31.5955	34.0	31.5	35.0	25.0	35.0
90-91	31.394750000000002	34.0	31.0	35.0	25.0	35.0
92-93	31.081125	34.0	31.0	35.0	24.5	35.0
94-95	30.747374999999998	34.0	31.0	35.0	23.5	35.0
96-97	30.466875	34.0	31.0	35.0	23.0	35.0
98-99	30.082875	34.0	30.0	35.0	20.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	0.0
15	3.0
16	5.0
17	3.0
18	5.0
19	12.0
20	6.0
21	7.0
22	11.0
23	11.0
24	20.0
25	24.0
26	37.0
27	54.0
28	64.0
29	83.0
30	106.0
31	144.0
32	173.0
33	227.0
34	330.0
35	401.0
36	615.0
37	965.0
38	671.0
39	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.075	22.55	33.025	25.35
2	20.769424189087253	25.99949710837314	26.04978627105859	27.181292431481012
3	25.874999999999996	30.825000000000003	17.599999999999998	25.7
4	21.880470117529384	39.28482120530133	21.45536384096024	17.37934483620905
5	18.4	35.775	26.200000000000003	19.625
6	20.5	16.55	42.225	20.724999999999998
7	20.325	23.25	31.6	24.825
8	20.05	22.875	32.025	25.05
9	21.4	41.575	20.05	16.975
10-11	22.3875	27.0875	24.85	25.674999999999997
12-13	20.825	28.575	31.137500000000003	19.4625
14-15	21.1375	25.2625	29.349999999999998	24.25
16-17	20.875	26.6	28.262500000000003	24.2625
18-19	24.8625	23.3625	28.65	23.125
20-21	25.5	28.599999999999998	25.687500000000004	20.2125
22-23	24.887500000000003	25.374999999999996	29.3375	20.4
24-25	22.6125	30.5	23.65	23.2375
26-27	23.125	25.55	25.0375	26.2875
28-29	20.962500000000002	26.174999999999997	25.324999999999996	27.537499999999998
30-31	21.3875	27.825	26.687499999999996	24.099999999999998
32-33	21.0125	26.687499999999996	28.6125	23.6875
34-35	21.6125	24.65	29.0875	24.65
36-37	23.4875	29.775000000000002	25.35	21.3875
38-39	24.4	24.4875	28.6875	22.425
40-41	23.875	29.5875	26.875	19.662499999999998
42-43	22.5	26.950000000000003	26.5125	24.0375
44-45	22.3125	27.700000000000003	27.325	22.662499999999998
46-47	24.640580072509064	24.753094136767096	25.790723840480062	24.815601950243778
48-49	21.875	30.2625	24.5	23.3625
50-51	22.5125	25.624999999999996	27.6625	24.2
52-53	22.625	25.6	29.812499999999996	21.9625
54-55	20.5375	25.2	27.3375	26.924999999999997
56-57	20.525	24.2375	30.7375	24.5
58-59	23.1875	29.675	28.175	18.9625
60-61	27.487499999999997	26.25	22.875	23.3875
62-63	22.95	22.525000000000002	30.85	23.674999999999997
64-65	23.3375	26.8375	27.762500000000003	22.0625
66-67	21.1625	25.025	29.1125	24.7
68-69	23.9125	28.3375	25.2625	22.4875
70-71	21.8	26.787499999999998	25.337500000000002	26.075
72-73	24.474999999999998	26.950000000000003	25.275	23.3
74-75	25.2875	27.425	25.8125	21.475
76-77	23.51689612015019	29.374217772215268	24.93116395494368	22.177722152690862
78-79	24.275	25.124999999999996	27.575	23.025000000000002
80-81	21.825	26.8	27.0125	24.3625
82-83	20.3625	24.325	34.1625	21.15
84-85	22.2125	26.424999999999997	26.2875	25.074999999999996
86-87	20.95	28.125	27.962500000000002	22.9625
88-89	23.5875	27.625	26.9125	21.875
90-91	23.25	28.1625	25.4375	23.150000000000002
92-93	23.425	27.737499999999997	25.025	23.8125
94-95	23.525	26.400000000000002	24.8125	25.2625
96-97	23.799999999999997	28.6125	23.025000000000002	24.5625
98-99	21.762500000000003	23.1375	30.7125	24.3875
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.0
33	1.0
34	1.5
35	2.5
36	2.5
37	4.5
38	7.5
39	23.0
40	51.5
41	111.0
42	235.0
43	312.0
44	407.0
45	576.0
46	672.0
47	551.0
48	331.0
49	243.5
50	193.0
51	131.5
52	86.0
53	39.5
54	12.0
55	4.5
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.575
3	0.0
4	0.025
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0125
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.125
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
99	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.8670322138451	24.025
2	10.28101439342015	7.5
3	6.579849211788896	7.199999999999999
4	3.358464701850582	4.9
5	2.878684030157642	5.25
6	1.9876627827278959	4.35
7	1.233721727210418	3.15
8	0.822481151473612	2.4
9	1.0966415352981496	3.5999999999999996
>10	5.894448252227553	37.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATG	44	1.0999999999999999	No Hit
TCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCAC	43	1.075	No Hit
CCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAA	42	1.05	No Hit
CACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGC	40	1.0	No Hit
AGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCA	39	0.975	No Hit
TTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCC	30	0.75	No Hit
CAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAG	28	0.7000000000000001	No Hit
CCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAAT	28	0.7000000000000001	No Hit
TCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATG	28	0.7000000000000001	No Hit
GCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCT	26	0.65	No Hit
GCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGT	25	0.625	No Hit
TGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTG	24	0.6	No Hit
CCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAA	23	0.575	No Hit
TAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATC	22	0.5499999999999999	No Hit
TCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATT	22	0.5499999999999999	No Hit
AGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAGC	21	0.525	No Hit
CTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAAT	21	0.525	No Hit
CAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTC	21	0.525	No Hit
TTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAA	21	0.525	No Hit
TTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGG	21	0.525	No Hit
AAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAA	20	0.5	No Hit
AAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGG	20	0.5	No Hit
CAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCA	20	0.5	No Hit
TTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCG	20	0.5	No Hit
GACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAACT	20	0.5	No Hit
AAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGG	19	0.475	No Hit
TTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCT	19	0.475	No Hit
TGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACC	19	0.475	No Hit
CAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAA	19	0.475	No Hit
GCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTG	18	0.44999999999999996	No Hit
TAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAG	18	0.44999999999999996	No Hit
GAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTG	18	0.44999999999999996	No Hit
AGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATG	18	0.44999999999999996	No Hit
CCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTT	17	0.42500000000000004	No Hit
TTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTT	17	0.42500000000000004	No Hit
GAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCA	16	0.4	No Hit
CCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCT	16	0.4	No Hit
TTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACG	16	0.4	No Hit
AGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTGC	16	0.4	No Hit
TGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTT	16	0.4	No Hit
TCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTA	16	0.4	No Hit
GTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAA	15	0.375	No Hit
CCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTA	15	0.375	No Hit
CGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAAC	15	0.375	No Hit
TACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGA	15	0.375	No Hit
TCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTT	15	0.375	No Hit
TCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGG	15	0.375	No Hit
CAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAAT	15	0.375	No Hit
CGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTAC	15	0.375	No Hit
GTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACC	14	0.35000000000000003	No Hit
AGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCT	14	0.35000000000000003	No Hit
CAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTA	14	0.35000000000000003	No Hit
TTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACC	14	0.35000000000000003	No Hit
TTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCA	14	0.35000000000000003	No Hit
TCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGT	14	0.35000000000000003	No Hit
TTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGT	14	0.35000000000000003	No Hit
CCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGG	13	0.325	No Hit
AAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATG	13	0.325	No Hit
CCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAA	13	0.325	No Hit
TTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAA	13	0.325	No Hit
TTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATT	13	0.325	No Hit
TAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAAC	13	0.325	No Hit
TCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGG	13	0.325	No Hit
AGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGC	13	0.325	No Hit
CTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCA	12	0.3	No Hit
AACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCT	12	0.3	No Hit
CTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCA	11	0.27499999999999997	No Hit
ATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCC	11	0.27499999999999997	No Hit
AATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCG	11	0.27499999999999997	No Hit
CCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGT	11	0.27499999999999997	No Hit
CAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGT	11	0.27499999999999997	No Hit
TTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCAT	11	0.27499999999999997	No Hit
TGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTT	11	0.27499999999999997	No Hit
CTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAA	11	0.27499999999999997	No Hit
GCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCA	11	0.27499999999999997	No Hit
GGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGT	11	0.27499999999999997	No Hit
ACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGC	11	0.27499999999999997	No Hit
ATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAACTTACCAA	11	0.27499999999999997	No Hit
CACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTC	10	0.25	No Hit
TCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTG	10	0.25	No Hit
TCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTT	10	0.25	No Hit
CGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAA	10	0.25	No Hit
TGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGA	10	0.25	No Hit
TTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCAT	10	0.25	No Hit
GGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAA	10	0.25	No Hit
TTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATT	10	0.25	No Hit
TTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAG	9	0.22499999999999998	No Hit
AACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATG	9	0.22499999999999998	No Hit
GAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAG	9	0.22499999999999998	No Hit
TTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAG	9	0.22499999999999998	No Hit
TTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGG	9	0.22499999999999998	No Hit
CTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTG	9	0.22499999999999998	No Hit
CAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCT	9	0.22499999999999998	No Hit
GGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCT	9	0.22499999999999998	No Hit
TTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTC	9	0.22499999999999998	No Hit
ACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATT	9	0.22499999999999998	No Hit
CCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAG	9	0.22499999999999998	No Hit
AAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCAT	9	0.22499999999999998	No Hit
CTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGC	9	0.22499999999999998	No Hit
AACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGAT	9	0.22499999999999998	No Hit
CAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGC	9	0.22499999999999998	No Hit
GAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGAA	9	0.22499999999999998	No Hit
TAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTG	8	0.2	No Hit
GATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAGCC	8	0.2	No Hit
CAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGA	8	0.2	No Hit
CGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTA	8	0.2	No Hit
GCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGT	8	0.2	No Hit
GAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGC	8	0.2	No Hit
CTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATC	8	0.2	No Hit
CACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGGGAA	8	0.2	No Hit
TCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCT	8	0.2	No Hit
GGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGA	8	0.2	No Hit
CTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCAT	8	0.2	No Hit
CCCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAA	8	0.2	No Hit
GCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAG	7	0.17500000000000002	No Hit
TTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTTTGTAGA	7	0.17500000000000002	No Hit
GCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATT	7	0.17500000000000002	No Hit
AAAAAGACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAGA	7	0.17500000000000002	No Hit
TCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTC	7	0.17500000000000002	No Hit
AGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAA	7	0.17500000000000002	No Hit
GCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTT	7	0.17500000000000002	No Hit
GGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTC	7	0.17500000000000002	No Hit
AAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGG	7	0.17500000000000002	No Hit
TTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCT	7	0.17500000000000002	No Hit
TCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGC	7	0.17500000000000002	No Hit
CCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTG	7	0.17500000000000002	No Hit
AGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGAT	7	0.17500000000000002	No Hit
TCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGA	7	0.17500000000000002	No Hit
TTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGC	7	0.17500000000000002	No Hit
CAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTG	7	0.17500000000000002	No Hit
TGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTT	7	0.17500000000000002	No Hit
GCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTG	7	0.17500000000000002	No Hit
CCAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGT	6	0.15	No Hit
GCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCT	6	0.15	No Hit
TAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGG	6	0.15	No Hit
AAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCG	6	0.15	No Hit
AGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCA	6	0.15	No Hit
TTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCT	6	0.15	No Hit
AGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTC	6	0.15	No Hit
GAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGC	6	0.15	No Hit
TGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGT	6	0.15	No Hit
ATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGA	6	0.15	No Hit
GGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGC	6	0.15	No Hit
AACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAAT	6	0.15	No Hit
CTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAA	6	0.15	No Hit
AAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGT	6	0.15	No Hit
TGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGAC	6	0.15	No Hit
TCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATG	6	0.15	No Hit
GGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGC	6	0.15	No Hit
CCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTCAT	6	0.15	No Hit
TGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACC	6	0.15	No Hit
CTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTG	6	0.15	No Hit
GACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTA	6	0.15	No Hit
TTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAAT	6	0.15	No Hit
AACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCT	6	0.15	No Hit
ACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCA	6	0.15	No Hit
CCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACC	6	0.15	No Hit
GCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAA	6	0.15	No Hit
ACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCC	6	0.15	No Hit
TGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCA	6	0.15	No Hit
TTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGA	6	0.15	No Hit
AAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATC	5	0.125	No Hit
CTCAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGC	5	0.125	No Hit
TTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAAT	5	0.125	No Hit
CAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGC	5	0.125	No Hit
GGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAA	5	0.125	No Hit
CGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTAC	5	0.125	No Hit
GGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCT	5	0.125	No Hit
TCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGA	5	0.125	No Hit
AAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAG	5	0.125	No Hit
TCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGAC	5	0.125	No Hit
CTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGC	5	0.125	No Hit
GTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCT	5	0.125	No Hit
CTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGA	5	0.125	No Hit
GTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAA	5	0.125	No Hit
TTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCG	5	0.125	No Hit
GGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTG	5	0.125	No Hit
TTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATAC	5	0.125	No Hit
TCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGGG	5	0.125	No Hit
GGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATC	5	0.125	No Hit
AATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGAT	5	0.125	No Hit
AGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAAC	5	0.125	No Hit
AAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGA	5	0.125	No Hit
TTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAG	5	0.125	No Hit
ATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCT	5	0.125	No Hit
AAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGG	5	0.125	No Hit
CCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAA	5	0.125	No Hit
AAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTA	5	0.125	No Hit
TCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGAT	5	0.125	No Hit
TGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAG	5	0.125	No Hit
TGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAA	5	0.125	No Hit
AATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAA	5	0.125	No Hit
TGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGC	5	0.125	No Hit
CCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTA	5	0.125	No Hit
GAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTT	5	0.125	No Hit
GGCAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTC	5	0.125	No Hit
TTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAG	5	0.125	No Hit
AATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCAT	5	0.125	No Hit
TCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCA	5	0.125	No Hit
AGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTT	5	0.125	No Hit
ATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCC	5	0.125	No Hit
AACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCC	5	0.125	No Hit
ATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25558505 read2 length is 99 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558505_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3999
Sequences flagged as poor quality	0
Sequence length	99
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.858714678669667	31.0	30.0	34.0	26.0	34.0
2	30.19429857464366	31.0	30.0	34.0	26.0	34.0
3	33.78794698674669	35.0	35.0	37.0	28.0	37.0
4	33.71217804451113	35.0	35.0	37.0	28.0	37.0
5	33.76569142285572	35.0	35.0	37.0	28.0	37.0
6	33.87846961740435	36.0	35.0	37.0	29.0	37.0
7	34.003500875218805	36.0	35.0	37.0	29.0	37.0
8	35.43485871467867	38.0	35.0	39.0	29.0	39.0
9	35.48462115528882	38.0	35.0	39.0	29.0	39.0
10-11	35.66254063515879	38.0	35.0	39.0	29.5	39.0
12-13	35.954613653413354	38.5	35.5	40.0	29.0	40.0
14-15	36.624281070267564	39.0	36.0	41.0	30.0	41.0
16-17	36.612528132033006	39.0	36.0	41.0	30.0	41.0
18-19	36.61477869467367	39.0	35.5	41.0	29.5	41.0
20-21	36.642035508877214	39.0	36.0	41.0	29.5	41.0
22-23	36.326956739184794	39.0	35.0	41.0	29.0	41.0
24-25	36.46499124781195	39.0	35.0	41.0	29.5	41.0
26-27	36.469617404351084	39.0	35.0	41.0	29.5	41.0
28-29	36.42873218304576	39.0	35.0	40.0	30.0	41.0
30-31	36.31245311327832	39.0	35.0	41.0	28.5	41.0
32-33	36.04801200300075	39.0	35.0	40.0	28.5	41.0
34-35	36.03588397099274	38.5	34.5	40.0	29.0	41.0
36-37	35.668417104276074	38.0	34.0	40.0	27.5	41.0
38-39	35.79769942485622	38.0	34.0	40.0	28.5	41.0
40-41	35.58902225556389	38.0	34.0	40.0	27.5	41.0
42-43	35.587396849212304	38.0	34.0	40.0	27.5	41.0
44-45	35.525381345336335	38.0	33.5	40.0	27.5	41.0
46-47	35.551637909477364	38.0	33.5	40.0	27.5	41.0
48-49	35.3858464616154	38.0	33.0	40.0	27.0	41.0
50-51	33.76156539134784	36.5	32.0	38.5	25.0	39.5
52-53	34.94498624656164	38.0	33.0	39.5	27.5	40.5
54-55	35.06914228557139	38.0	33.0	40.0	27.0	41.0
56-57	35.520255063765944	38.0	34.0	40.0	28.0	41.0
58-59	35.17404351087772	38.0	34.0	40.0	27.0	41.0
60-61	35.168792198049516	38.0	34.0	40.0	28.0	41.0
62-63	34.736434108527135	37.0	33.0	39.5	27.5	41.0
64-65	34.17016754188547	37.0	33.0	39.0	26.0	40.0
66-67	33.62690672668167	36.0	32.0	39.0	26.0	40.0
68-69	33.172168042010505	35.5	32.0	37.5	26.0	39.0
70-71	32.896224056014006	35.0	32.0	37.0	26.0	39.0
72-73	32.434108527131784	35.0	32.0	37.0	26.0	38.5
74-75	31.959739934983745	35.0	31.0	36.0	26.0	37.0
76-77	31.62578144536134	34.0	31.0	35.5	25.5	37.0
78-79	31.133783445861468	34.0	31.0	35.0	23.0	36.0
80-81	30.82845711427857	34.0	31.0	35.0	22.5	36.0
82-83	30.817454363590898	34.0	31.0	35.0	23.0	36.0
84-85	30.4031007751938	34.0	30.0	35.0	20.0	35.5
86-87	30.322705676419105	34.0	30.0	35.0	20.0	35.0
88-89	30.27956989247312	34.0	30.0	35.0	20.0	35.0
90-91	30.072643160790197	34.0	30.0	35.0	19.0	35.0
92-93	29.873593398349588	34.0	30.0	35.0	18.0	35.0
94-95	29.551012753188296	34.0	30.0	35.0	10.0	35.0
96-97	29.25831457864466	34.0	30.0	35.0	2.0	35.0
98-99	29.035758939734933	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22.0
3	4.0
4	2.0
5	2.0
6	4.0
7	4.0
8	6.0
9	6.0
10	7.0
11	9.0
12	11.0
13	4.0
14	8.0
15	13.0
16	17.0
17	25.0
18	22.0
19	17.0
20	18.0
21	13.0
22	26.0
23	22.0
24	25.0
25	42.0
26	54.0
27	63.0
28	88.0
29	101.0
30	134.0
31	142.0
32	191.0
33	234.0
34	282.0
35	354.0
36	491.0
37	897.0
38	617.0
39	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.08788111708942	20.59953881629516	35.17806815270305	25.134511913912377
2	20.52052052052052	23.923923923923923	28.503503503503502	27.05205205205205
3	26.376376376376378	30.130130130130127	18.06806806806807	25.425425425425423
4	21.93954659949622	37.27959697732997	22.090680100755666	18.690176322418136
5	19.004751187796952	35.43385846461615	26.231557889472366	19.32983245811453
6	19.167717528373267	15.10718789407314	44.867591424968474	20.857503152585117
7	20.955238809702426	22.080520130032507	33.18329582395599	23.78094523630908
8	20.530132533133283	22.005501375343837	33.23330832708177	24.23105776444111
9	21.13028257064266	43.33583395848962	18.929732433108278	16.60415103775944
10-11	23.74343585896474	24.99374843710928	25.468867216804203	25.79394848712178
12-13	21.355338834708675	27.406851712928233	31.782945736434108	19.454863715928983
14-15	20.64266066516629	24.93123280820205	30.532633158289574	23.893473368342086
16-17	20.980245061265315	25.868967241810452	28.91972993248312	24.23105776444111
18-19	23.243310827706924	24.131032758189548	28.59464866216554	24.031007751937985
20-21	25.4411212614191	27.81879614566387	25.653860593167316	21.086221999749718
22-23	24.768576432324245	24.74355766825119	30.673004753565174	19.814861145859393
24-25	21.99299824956239	29.957489372343087	24.33108277069267	23.718429607401852
26-27	23.355838959739934	25.35633908477119	25.11877969492373	26.16904226056514
28-29	20.130032508127034	27.094273568392097	25.868967241810452	26.906726681670417
30-31	21.99299824956239	28.257064266066518	25.881470367591895	23.868467116779193
32-33	21.92587027297771	26.270974204858504	27.848735286751815	23.954420235411973
34-35	22.145804676753784	25.009378516943855	28.210578967112664	24.634237839189694
36-37	23.455863965991497	29.232308077019255	25.04376094023506	22.268067016754188
38-39	23.733900212579716	24.121545579592347	29.736151056646243	22.408403151181695
40-41	24.131032758189548	29.394848712178046	26.16904226056514	20.305076269067268
42-43	22.29307326831708	27.406851712928233	26.76919229807452	23.53088272068017
44-45	23.018254563640912	27.35683920980245	27.44436109027257	22.18054513628407
46-47	24.518629657414355	23.980995248812203	26.431607901975497	25.068767191797946
48-49	21.85546386596649	30.807701925481368	24.831207801950487	22.50562640660165
50-51	22.468117029257314	24.99374843710928	27.544386096524132	24.99374843710928
52-53	21.680420105026258	27.094273568392097	29.107276819204802	22.118029507376843
54-55	20.64266066516629	25.881470367591895	27.831957989497376	25.64391097774444
56-57	21.467866966741685	23.605901475368842	30.820205051262818	24.10602650662666
58-59	22.818204551137786	29.34483620905226	28.89472368092023	18.942235558889724
60-61	26.65666416604151	26.494123530882717	23.143285821455365	23.705926481620406
62-63	22.918229557389346	21.630407601900476	31.820455113778443	23.63090772693173
64-65	23.93098274568642	27.094273568392097	26.144036009002253	22.83070767691923
66-67	21.405351337834457	23.468367091772944	29.16979244811203	25.95648912228057
68-69	22.455613903475868	28.28207051762941	26.069017254313575	23.193298324581146
70-71	20.505126281570394	26.881720430107524	25.49387346836709	27.11927981995499
72-73	25.206301575393848	25.943985996499126	25.056264066016503	23.793448362090523
74-75	24.356089022255563	27.169292323080768	27.44436109027257	21.030257564391096
76-77	24.06851712928232	28.982245561390346	24.418604651162788	22.53063265816454
78-79	24.643660915228807	24.99374843710928	29.08227056764191	21.280320080020005
80-81	22.04576716268601	26.710016256096036	25.909716143553833	25.334500437664126
82-83	19.86746686671668	24.81870467616904	34.058514628657164	21.255313828457115
84-85	21.930482620655166	26.39409852463116	27.00675168792198	24.668667166791696
86-87	20.442610652663166	28.169542385596397	28.569642410602654	22.818204551137786
88-89	22.74318579644911	27.24431107776944	27.719429857464366	22.29307326831708
90-91	23.643410852713178	28.219554888722183	25.11877969492373	23.018254563640912
92-93	23.393348337084273	27.819454863715933	24.918729682420604	23.868467116779193
94-95	25.143785946486624	24.60615153788447	25.30632658164541	24.943735933983497
96-97	23.968492123030757	27.46936734183546	22.58064516129032	25.98149537384346
98-99	21.742935733933482	23.268317079269817	30.48262065516379	24.50612653163291
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.5
34	1.0
35	1.0
36	3.5
37	6.5
38	14.0
39	35.0
40	65.0
41	97.5
42	211.5
43	302.5
44	409.5
45	578.0
46	650.5
47	543.0
48	340.0
49	253.0
50	208.0
51	142.0
52	81.5
53	33.5
54	13.0
55	6.0
56	1.0
57	1.0
58	1.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4006001500375094
2	0.07501875468867217
3	0.07501875468867217
4	0.7251812953238309
5	0.0
6	0.8502125531382845
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.08752188047011752
22-23	0.05001250312578145
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.15003750937734434
34-35	0.012503125781445362
36-37	0.0
38-39	0.012503125781445362
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.012503125781445362
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
99	3999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.360840210052515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9515570934256	32.93323330832708
2	6.920415224913495	6.001500375093773
3	3.517877739331027	4.576144036009002
4	2.306805074971165	4.001000250062516
5	2.1337946943483272	4.626156539134783
6	1.6724336793540944	4.351087771942986
7	1.384083044982699	4.201050262565642
8	0.6343713956170703	2.2005501375343837
9	0.6343713956170703	2.4756189047261814
>10	4.844290657439446	34.63365841460365
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCA	44	1.1002750687671918	No Hit
AGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATG	39	0.9752438109527383	No Hit
CACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGC	36	0.9002250562640659	No Hit
CCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAA	34	0.8502125531382845	No Hit
TTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCG	32	0.8002000500125032	No Hit
TCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCAC	32	0.8002000500125032	No Hit
TCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATG	31	0.7751937984496124	No Hit
CCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAAT	29	0.7251812953238309	No Hit
CTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAAT	24	0.6001500375093773	No Hit
CAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAA	24	0.6001500375093773	No Hit
GACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAACT	24	0.6001500375093773	No Hit
GCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCT	23	0.5751437859464866	No Hit
TTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGG	23	0.5751437859464866	No Hit
TTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACC	22	0.5501375343835959	No Hit
CGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTAC	21	0.5251312828207052	No Hit
TAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATC	21	0.5251312828207052	No Hit
TTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCC	21	0.5251312828207052	No Hit
TGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACC	20	0.5001250312578145	No Hit
AAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGG	20	0.5001250312578145	No Hit
CAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAG	19	0.4751187796949237	No Hit
TCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGG	19	0.4751187796949237	No Hit
CCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGG	18	0.45011252813203295	No Hit
GAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCA	18	0.45011252813203295	No Hit
AAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAA	18	0.45011252813203295	No Hit
TACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGA	18	0.45011252813203295	No Hit
TCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATT	18	0.45011252813203295	No Hit
CCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAA	18	0.45011252813203295	No Hit
CCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTA	17	0.42510627656914224	No Hit
TGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTG	17	0.42510627656914224	No Hit
CAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCA	17	0.42510627656914224	No Hit
TGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTT	16	0.4001000250062516	No Hit
CCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCT	16	0.4001000250062516	No Hit
CCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTT	16	0.4001000250062516	No Hit
TTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCA	15	0.3750937734433608	No Hit
TTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGT	15	0.3750937734433608	No Hit
GAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGC	15	0.3750937734433608	No Hit
CTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAA	15	0.3750937734433608	No Hit
TTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAA	15	0.3750937734433608	No Hit
TTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACG	15	0.3750937734433608	No Hit
AGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGC	15	0.3750937734433608	No Hit
TTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAG	14	0.3500875218804701	No Hit
TCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTG	14	0.3500875218804701	No Hit
CTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTG	14	0.3500875218804701	No Hit
AGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATG	14	0.3500875218804701	No Hit
TTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTG	14	0.3500875218804701	No Hit
GTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACC	13	0.3250812703175794	No Hit
CTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCA	13	0.3250812703175794	No Hit
CTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAA	13	0.3250812703175794	No Hit
TTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAA	13	0.3250812703175794	No Hit
GGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAA	13	0.3250812703175794	No Hit
AAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGA	13	0.3250812703175794	No Hit
AGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTGC	13	0.3250812703175794	No Hit
CAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAAT	13	0.3250812703175794	No Hit
TTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTT	13	0.3250812703175794	No Hit
GGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCT	12	0.30007501875468867	No Hit
TCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCT	12	0.30007501875468867	No Hit
ACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGC	12	0.30007501875468867	No Hit
CAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCT	12	0.30007501875468867	No Hit
GGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGC	12	0.30007501875468867	No Hit
GAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTG	12	0.30007501875468867	No Hit
TTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCT	12	0.30007501875468867	No Hit
GCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGT	12	0.30007501875468867	No Hit
AGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAGC	11	0.27506876719179796	No Hit
GCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTG	11	0.27506876719179796	No Hit
GAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGC	11	0.27506876719179796	No Hit
TAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACC	11	0.27506876719179796	No Hit
TCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTT	11	0.27506876719179796	No Hit
TGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTT	11	0.27506876719179796	No Hit
TCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTA	11	0.27506876719179796	No Hit
GCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTT	11	0.27506876719179796	No Hit
GTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAA	11	0.27506876719179796	No Hit
TAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAG	11	0.27506876719179796	No Hit
CTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATC	11	0.27506876719179796	No Hit
AAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGG	11	0.27506876719179796	No Hit
GCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAG	10	0.25006251562890724	No Hit
AAAAAGACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAGA	10	0.25006251562890724	No Hit
CCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGT	10	0.25006251562890724	No Hit
TGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAG	10	0.25006251562890724	No Hit
CACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTC	10	0.25006251562890724	No Hit
AATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCG	10	0.25006251562890724	No Hit
TCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTT	10	0.25006251562890724	No Hit
CAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTC	10	0.25006251562890724	No Hit
AAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATG	10	0.25006251562890724	No Hit
CTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCAT	10	0.25006251562890724	No Hit
GCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACT	9	0.22505626406601648	No Hit
ATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCC	9	0.22505626406601648	No Hit
TAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTG	9	0.22505626406601648	No Hit
TCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTC	9	0.22505626406601648	No Hit
TTGTCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCAC	9	0.22505626406601648	No Hit
TCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGT	9	0.22505626406601648	No Hit
TGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAA	9	0.22505626406601648	No Hit
CGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTA	9	0.22505626406601648	No Hit
GGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGT	9	0.22505626406601648	No Hit
TTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATT	9	0.22505626406601648	No Hit
TGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTT	9	0.22505626406601648	No Hit
GTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAG	8	0.2000500125031258	No Hit
GTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGA	8	0.2000500125031258	No Hit
TGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGT	8	0.2000500125031258	No Hit
CCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAA	8	0.2000500125031258	No Hit
TTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGC	8	0.2000500125031258	No Hit
TGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTT	8	0.2000500125031258	No Hit
AGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCT	8	0.2000500125031258	No Hit
GCCAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGG	8	0.2000500125031258	No Hit
TCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGAT	8	0.2000500125031258	No Hit
GGGTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCC	8	0.2000500125031258	No Hit
CAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTG	8	0.2000500125031258	No Hit
TTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTTTGTAGA	7	0.17504376094023505	No Hit
CTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGC	7	0.17504376094023505	No Hit
TGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGA	7	0.17504376094023505	No Hit
GCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGT	7	0.17504376094023505	No Hit
ATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGA	7	0.17504376094023505	No Hit
AAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGT	7	0.17504376094023505	No Hit
GTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGAT	7	0.17504376094023505	No Hit
CCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGT	7	0.17504376094023505	No Hit
ACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCC	7	0.17504376094023505	No Hit
TTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACC	7	0.17504376094023505	No Hit
TCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATG	7	0.17504376094023505	No Hit
TTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAG	7	0.17504376094023505	No Hit
TCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATA	7	0.17504376094023505	No Hit
TCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGAC	7	0.17504376094023505	No Hit
GTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTC	7	0.17504376094023505	No Hit
TTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCT	7	0.17504376094023505	No Hit
TGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGAC	7	0.17504376094023505	No Hit
ATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCT	7	0.17504376094023505	No Hit
TTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATT	7	0.17504376094023505	No Hit
TCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTAC	7	0.17504376094023505	No Hit
CTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGC	7	0.17504376094023505	No Hit
TTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAAT	7	0.17504376094023505	No Hit
ACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCC	7	0.17504376094023505	No Hit
GGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGA	7	0.17504376094023505	No Hit
GCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCC	6	0.15003750937734434	No Hit
TTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGG	6	0.15003750937734434	No Hit
CGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAA	6	0.15003750937734434	No Hit
GTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTT	6	0.15003750937734434	No Hit
CTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTG	6	0.15003750937734434	No Hit
CCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTA	6	0.15003750937734434	No Hit
TAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAAC	6	0.15003750937734434	No Hit
CAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGC	6	0.15003750937734434	No Hit
TTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAG	6	0.15003750937734434	No Hit
GAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGAA	6	0.15003750937734434	No Hit
AACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCC	6	0.15003750937734434	No Hit
AAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATC	6	0.15003750937734434	No Hit
AACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATG	6	0.15003750937734434	No Hit
CGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTT	6	0.15003750937734434	No Hit
AGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAA	6	0.15003750937734434	No Hit
AAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAG	6	0.15003750937734434	No Hit
TTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCAT	6	0.15003750937734434	No Hit
ATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATG	6	0.15003750937734434	No Hit
TTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGGGAAGATA	6	0.15003750937734434	No Hit
AACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAAT	6	0.15003750937734434	No Hit
TTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAG	6	0.15003750937734434	No Hit
TCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATG	6	0.15003750937734434	No Hit
TCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGC	6	0.15003750937734434	No Hit
GATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGG	6	0.15003750937734434	No Hit
CCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTCAT	6	0.15003750937734434	No Hit
AACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCT	6	0.15003750937734434	No Hit
GACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTA	6	0.15003750937734434	No Hit
TGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAAC	6	0.15003750937734434	No Hit
ATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCC	6	0.15003750937734434	No Hit
CTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCA	5	0.12503125781445362	No Hit
GCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATT	5	0.12503125781445362	No Hit
CAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGA	5	0.12503125781445362	No Hit
GGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTG	5	0.12503125781445362	No Hit
CCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGA	5	0.12503125781445362	No Hit
TTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATAC	5	0.12503125781445362	No Hit
CCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAA	5	0.12503125781445362	No Hit
CACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGGGAA	5	0.12503125781445362	No Hit
TGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGC	5	0.12503125781445362	No Hit
GGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTT	5	0.12503125781445362	No Hit
GCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTAT	5	0.12503125781445362	No Hit
AACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGAT	5	0.12503125781445362	No Hit
ATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGAC	5	0.12503125781445362	No Hit
TCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCA	5	0.12503125781445362	No Hit
GCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTG	5	0.12503125781445362	No Hit
TGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGA	5	0.12503125781445362	No Hit
TGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGC	5	0.12503125781445362	No Hit
TTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAAT	5	0.12503125781445362	No Hit
TACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCA	5	0.12503125781445362	No Hit
TAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGG	5	0.12503125781445362	No Hit
CCGGCTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAA	5	0.12503125781445362	No Hit
GTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCT	5	0.12503125781445362	No Hit
CTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGA	5	0.12503125781445362	No Hit
TTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTAT	5	0.12503125781445362	No Hit
TACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGAT	5	0.12503125781445362	No Hit
TTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTC	5	0.12503125781445362	No Hit
GCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCA	5	0.12503125781445362	No Hit
AGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGC	5	0.12503125781445362	No Hit
TCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGA	5	0.12503125781445362	No Hit
ACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCA	5	0.12503125781445362	No Hit
CCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACC	5	0.12503125781445362	No Hit
ATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCG	5	0.12503125781445362	No Hit
TCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGG	5	0.12503125781445362	No Hit
GAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTT	5	0.12503125781445362	No Hit
TGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCA	5	0.12503125781445362	No Hit
ATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACA	5	0.12503125781445362	No Hit
CTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCA	5	0.12503125781445362	No Hit
GTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAG	4	0.1000250062515629	No Hit
TTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGAATTAGATCCTCCA	4	0.1000250062515629	No Hit
GCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATC	4	0.1000250062515629	No Hit
CCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGGGA	4	0.1000250062515629	No Hit
TCCCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCA	4	0.1000250062515629	No Hit
AGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGT	4	0.1000250062515629	No Hit
CAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGT	4	0.1000250062515629	No Hit
GCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATC	4	0.1000250062515629	No Hit
TTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCG	4	0.1000250062515629	No Hit
TCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCC	4	0.1000250062515629	No Hit
ATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTT	4	0.1000250062515629	No Hit
AGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAAC	4	0.1000250062515629	No Hit
AAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTA	4	0.1000250062515629	No Hit
AAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCAT	4	0.1000250062515629	No Hit
GTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCT	4	0.1000250062515629	No Hit
TCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGA	4	0.1000250062515629	No Hit
GTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCG	4	0.1000250062515629	No Hit
TTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTT	4	0.1000250062515629	No Hit
GACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAAC	4	0.1000250062515629	No Hit
GCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAA	4	0.1000250062515629	No Hit
GGCAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTC	4	0.1000250062515629	No Hit
CTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGT	4	0.1000250062515629	No Hit
GAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAG	4	0.1000250062515629	No Hit
TTGAAGCGTTTCCCGACTGTATAGATCTCATGAATTAGATCCTCCATGCA	4	0.1000250062515629	No Hit
AAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCG	4	0.1000250062515629	No Hit
GATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAAGCC	4	0.1000250062515629	No Hit
ATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAG	4	0.1000250062515629	No Hit
GGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTC	4	0.1000250062515629	No Hit
CAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTA	4	0.1000250062515629	No Hit
CTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACT	4	0.1000250062515629	No Hit
TCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTT	4	0.1000250062515629	No Hit
GGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATC	4	0.1000250062515629	No Hit
CCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAG	4	0.1000250062515629	No Hit
CCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAA	4	0.1000250062515629	No Hit
CCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTG	4	0.1000250062515629	No Hit
CTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCT	4	0.1000250062515629	No Hit
ATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGT	4	0.1000250062515629	No Hit
GTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGT	4	0.1000250062515629	No Hit
CCCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAA	4	0.1000250062515629	No Hit
TGTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGA	4	0.1000250062515629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 17 READS because READLEN < 1
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Rejected 15 READS because READLEN < 1
Read 540 spots for SRR25558505.sra
Written 540 spots for SRR25558505.sra
Rejected 7 READS because READLEN < 1
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Rejected 1 READS because READLEN < 1
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Rejected 1 READS because READLEN < 1
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Rejected 1 READS because READLEN < 1
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Rejected 2 READS because READLEN < 1
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
Read 535 spots for SRR25558505.sra
Written 535 spots for SRR25558505.sra
SRR ids: ['SRR25558505.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_k7dd8rgi
SRR25558505.sra spots: 8565
blocks: [[1, 535], [536, 1070], [1071, 1605], [1606, 2140], [2141, 2675], [2676, 3210], [3211, 3745], [3746, 4280], [4281, 4815], [4816, 5350], [5351, 5885], [5886, 6420], [6421, 6955], [6956, 7490], [7491, 8025], [8026, 8565]]
SRR25558505 file size 1
SRR25558505 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25558505 SRR25558505_1.fastq SRR25558505_2.fastq
Input file:	SRR25558505_1.fastq
Paired file:	SRR25558505_2.fastq
trimmed:	SRR25558505-trimmed-pair1.fastq, SRR25558505-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 15:32:40 2026 >> started

Sun May 24 15:32:40 2026 >> done (0.021s)
8533 read pairs processed; of these:
  24 ( 0.28%) short read pairs filtered out after trimming by size control
  51 ( 0.60%) empty read pairs filtered out after trimming by size control
8458 (99.12%) read pairs available; of these:
1873 (22.14%) trimmed read pairs available after processing
6585 (77.86%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 52	   1	  0.01%
 53	   0	  0.00%
 54	   0	  0.00%
 55	   0	  0.00%
 56	   0	  0.00%
 57	   2	  0.02%
 58	   3	  0.04%
 59	   3	  0.04%
 60	   3	  0.04%
 61	   3	  0.04%
 62	   5	  0.06%
 63	   3	  0.04%
 64	   8	  0.09%
 65	  12	  0.14%
 66	   8	  0.09%
 67	  10	  0.12%
 68	  13	  0.15%
 69	  17	  0.20%
 70	  14	  0.17%
 71	  18	  0.21%
 72	  10	  0.12%
 73	  19	  0.22%
 74	  12	  0.14%
 75	  19	  0.22%
 76	  19	  0.22%
 77	  17	  0.20%
 78	  32	  0.38%
 79	  27	  0.32%
 80	  27	  0.32%
 81	  38	  0.45%
 82	  31	  0.37%
 83	  35	  0.41%
 84	  39	  0.46%
 85	  56	  0.66%
 86	  44	  0.52%
 87	  32	  0.38%
 88	  35	  0.41%
 89	  45	  0.53%
 90	  69	  0.82%
 91	  68	  0.80%
 92	  75	  0.89%
 93	  78	  0.92%
 94	 114	  1.35%
 95	 150	  1.77%
 96	 199	  2.35%
 97	 253	  2.99%
 98	 207	  2.45%
 99	6585	 77.86%
8458 reads passed initial QC


criterion=sequence-density
sequence-density=9.75
sequence-density-rank=1
fanout-score=2.02
fanout-score-rank=5
prefix-density=9.98
prefix-fanout=2.0
sequence=CTCAAGAAAAAG


criterion=fanout-score
sequence-density=9.21
sequence-density-rank=2
fanout-score=5.81
fanout-score-rank=1
prefix-density=18.83
prefix-fanout=2.8
sequence=AAGAAAAAGGTTCC


criterion=sequence-density
sequence-density=8.03
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=4
prefix-density=8.29
prefix-fanout=2.0
sequence=CTCAAGAAAAAG


criterion=fanout-score
sequence-density=7.76
sequence-density-rank=2
fanout-score=6.03
fanout-score-rank=1
prefix-density=15.96
prefix-fanout=2.9
sequence=AAGAAAAAGGTTCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x CTCAAGAAAAAG -y CTCAAGAAAAAG -o SRR25558505 SRR25558505_1.fastq SRR25558505_2.fastq
Input file:	SRR25558505_1.fastq
Paired file:	SRR25558505_2.fastq
trimmed:	SRR25558505-trimmed-pair1.fastq, SRR25558505-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTCAAGAAAAAG
-- paired 3' end adapter sequence (-y):	CTCAAGAAAAAG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 15:32:42 2026 >> started

Sun May 24 15:32:42 2026 >> done (0.012s)
6767 read pairs processed; of these:
   3 ( 0.04%) short read pairs filtered out after trimming by size control
  13 ( 0.19%) empty read pairs filtered out after trimming by size control
6751 (99.76%) read pairs available; of these:
   1 ( 0.01%) trimmed read pairs available after processing
6750 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 57	   2	  0.03%
 58	   3	  0.04%
 59	   2	  0.03%
 60	   2	  0.03%
 61	   2	  0.03%
 62	   3	  0.04%
 63	   3	  0.04%
 64	   6	  0.09%
 65	  10	  0.15%
 66	   6	  0.09%
 67	  10	  0.15%
 68	   9	  0.13%
 69	  15	  0.22%
 70	  10	  0.15%
 71	  14	  0.21%
 72	   8	  0.12%
 73	  17	  0.25%
 74	  10	  0.15%
 75	  14	  0.21%
 76	  15	  0.22%
 77	  10	  0.15%
 78	  25	  0.37%
 79	  21	  0.31%
 80	  21	  0.31%
 81	  33	  0.49%
 82	  22	  0.33%
 83	  29	  0.43%
 84	  31	  0.46%
 85	  43	  0.64%
 86	  35	  0.52%
 87	  24	  0.36%
 88	  27	  0.40%
 89	  36	  0.53%
 90	  53	  0.79%
 91	  53	  0.79%
 92	  52	  0.77%
 93	  59	  0.87%
 94	  91	  1.35%
 95	 124	  1.84%
 96	 159	  2.36%
 97	 204	  3.02%
 98	 163	  2.41%
 99	5275	 78.14%


criterion=sequence-density
sequence-density=9.57
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=5
prefix-density=9.90
prefix-fanout=2.0
sequence=CTCAAGAAAAAG


criterion=fanout-score
sequence-density=9.17
sequence-density-rank=2
fanout-score=5.84
fanout-score-rank=1
prefix-density=18.67
prefix-fanout=2.9
sequence=AAGAAAAAGGTTCC


criterion=sequence-density
sequence-density=7.91
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=4
prefix-density=8.26
prefix-fanout=2.0
sequence=CTCAAGAAAAAG


criterion=fanout-score
sequence-density=7.72
sequence-density-rank=2
fanout-score=6.06
fanout-score-rank=1
prefix-density=15.86
prefix-fanout=3.0
sequence=AAGAAAAAGGTTCC
SRR25558505 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 15:33:05
                             Started mapping on |	May 24 15:33:05
                                    Finished on |	May 24 15:33:08
       Mapping speed, Million of reads per hour |	10.13

                          Number of input reads |	8442
                      Average input read length |	193
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4604
                        Uniquely mapped reads % |	54.54%
                          Average mapped length |	192.63
                       Number of splices: Total |	5160
            Number of splices: Annotated (sjdb) |	5155
                       Number of splices: GT/AG |	5157
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	3
                      Mismatch rate per base, % |	0.44%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.09
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.33
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	102
             % of reads mapped to multiple loci |	1.21%
        Number of reads mapped to too many loci |	1
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	44.24%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3802	3802	3802
N_multimapping	102	102	102
N_noFeature	2	2229	2359
N_ambiguous	50	16	16
UnstrandedReadsAssigned:4552 PositiveStrandReadsAssigned:2359 NegativeStrandReadsAssigned:2229
Dataset is classified unstranded
MeadianReadLen=99 20thPercentileLength=99 echo kmer=95
SRR25558505 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558505-trimmed-pair1.fastq
                             SRR25558505-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 8,442 reads, 8,005 reads pseudoaligned
[quant] estimated average fragment length: 147.894
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 59 rounds

 109283 SRR25558505.ke.tsv
  52636 SRR25558505.se.tsv
 161919 total
==> SRR25558505.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25558505.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558505 completed mapping pipeline successfully
