Starting /dee2/code/volunteer_pipeline.sh SRR25558502
    current disk space = 460499947520
    free memory = 366561212 
SRR25558502 SRAfilesize
5b157297126d74e01e2041d32cfadcc7  SRR25558502.sra
SRR25558502.sra file validated
SRR25558502 is paired end
SRR25558502 is conventional basespace
SRR25558502 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3991
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.936356802806316	34.0	33.0	34.0	32.0	34.0
2	33.07592082184916	34.0	33.0	34.0	32.0	34.0
3	33.1292909045352	34.0	33.0	34.0	32.0	34.0
4	33.056627411676274	34.0	33.0	34.0	32.0	34.0
5	32.98371335504886	34.0	33.0	34.0	32.0	34.0
6	36.67326484590328	38.0	37.0	38.0	34.0	38.0
7	37.022550739163115	38.0	38.0	38.0	36.0	38.0
8	37.19368579303433	38.0	38.0	38.0	36.0	38.0
9	37.23127035830619	38.0	38.0	38.0	36.0	38.0
10-11	37.20859433725883	38.0	38.0	38.0	36.5	38.0
12-13	37.194813329992485	38.0	38.0	38.0	36.5	38.0
14-15	37.256076171385615	38.0	38.0	38.0	37.0	38.0
16-17	37.24404911049862	38.0	38.0	38.0	37.0	38.0
18-19	37.23928839889752	38.0	38.0	38.0	37.0	38.0
20-21	37.23690804309697	38.0	38.0	38.0	37.0	38.0
22-23	37.21661237785017	38.0	38.0	38.0	37.0	38.0
24-25	37.1701327987973	38.0	38.0	38.0	36.0	38.0
26-27	37.07892758707091	38.0	38.0	38.0	36.0	38.0
28-29	37.05023803558005	38.0	38.0	38.0	36.0	38.0
30-31	37.123778501628664	38.0	38.0	38.0	36.0	38.0
32-33	37.15447256326735	38.0	38.0	38.0	36.5	38.0
34-35	37.17827612127286	38.0	38.0	38.0	36.5	38.0
36-37	37.09609120521173	38.0	38.0	38.0	36.0	38.0
38-39	37.00112753695815	38.0	38.0	38.0	36.0	38.0
40-41	36.97920320721624	38.0	38.0	38.0	36.0	38.0
42-43	37.03532949135555	38.0	38.0	38.0	36.0	38.0
44-45	36.9736908043097	38.0	38.0	38.0	36.0	38.0
46-47	36.93735905788023	38.0	38.0	38.0	36.0	38.0
48-49	36.84302179904786	38.0	38.0	38.0	35.5	38.0
50-51	36.87797544475069	38.0	38.0	38.0	36.0	38.0
52-53	36.80280631420696	38.0	38.0	38.0	35.0	38.0
54-55	36.89438737158607	38.0	38.0	38.0	35.0	38.0
56-57	36.889877223753444	38.0	38.0	38.0	35.5	38.0
58-59	36.90691556001002	38.0	38.0	38.0	35.5	38.0
60-61	36.92696066148835	38.0	38.0	38.0	35.0	38.0
62-63	36.93222250062641	38.0	38.0	38.0	35.5	38.0
64-65	36.93397644700576	38.0	38.0	38.0	35.0	38.0
66-67	36.91969431220245	38.0	38.0	38.0	36.0	38.0
68-69	36.9119268353796	38.0	38.0	38.0	35.5	38.0
70-71	36.90729140566275	38.0	38.0	38.0	35.5	38.0
72-73	36.882861438236034	38.0	38.0	38.0	35.0	38.0
74-75	36.78000501127537	38.0	38.0	38.0	34.5	38.0
76-77	36.74668003006765	38.0	38.0	38.0	35.0	38.0
78-79	36.79328489100476	38.0	38.0	38.0	35.0	38.0
80-81	36.748433976447004	38.0	38.0	38.0	35.0	38.0
82-83	36.67551991981959	38.0	38.0	38.0	34.5	38.0
84-85	36.73139564019043	38.0	38.0	38.0	35.0	38.0
86-87	36.78125783011777	38.0	38.0	38.0	35.0	38.0
88-89	36.7119769481333	38.0	38.0	38.0	35.0	38.0
90-91	36.734402405412176	38.0	38.0	38.0	35.0	38.0
92-93	36.65146579804561	38.0	38.0	38.0	34.5	38.0
94-95	36.570909546479584	38.0	38.0	38.0	34.0	38.0
96-97	36.52442996742671	38.0	38.0	38.0	34.0	38.0
98-99	36.36657479328489	38.0	38.0	38.0	34.0	38.0
100-101	35.704460035078924	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	0.0
20	0.0
21	1.0
22	3.0
23	2.0
24	2.0
25	2.0
26	12.0
27	18.0
28	24.0
29	29.0
30	32.0
31	53.0
32	76.0
33	102.0
34	118.0
35	187.0
36	417.0
37	2909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.54618473895582	6.902610441767068	8.659638554216867	47.89156626506024
2	18.892508143322477	8.39388624404911	38.56176396893009	34.15184164369832
3	20.42094713104485	9.095464795790528	25.60761713856176	44.875970934602854
4	27.61212728639439	17.0383362565773	26.033575544976195	29.31596091205212
5	14.983713355048861	28.088198446504638	31.87171135053871	25.056376847907792
6	17.91530944625407	28.46404409922325	34.527687296416936	19.09295915810574
7	18.16587321473315	17.38912553244801	49.61162615885743	14.833375093961413
8	19.794537709847155	25.35705337008269	37.860185417188674	16.988223502881482
9	16.662490603858682	22.92658481583563	38.51165121523428	21.899273365071412
10-11	22.488098220997244	28.952643447757453	28.82736156351792	19.731896767727385
12-13	25.119017790027563	21.160110248058132	32.18491606113756	21.53595590077675
14-15	19.218241042345277	21.686294161864193	40.328238536707595	18.767226259082936
16-17	20.15785517414182	15.660235529942371	39.401152593335006	24.780756702580806
18-19	21.824104234527688	18.341267852668505	28.71460786770233	31.120020045101477
20-21	20.383362565772988	19.318466549736907	34.2645953395139	26.033575544976195
22-23	25.043848659483835	23.552994237033325	29.491355549987468	21.911801553495362
24-25	23.57805061388123	22.437985467301427	31.1951891756452	22.788774743172137
26-27	24.730643948884993	24.53019293410173	25.98346279128038	24.7557003257329
28-29	21.69882235028815	29.491355549987468	20.270608869957403	28.539213229766975
30-31	20.74668003006765	21.18516662490604	26.183913806063643	31.884239538962667
32-33	19.09295915810574	21.561012277624656	32.67351540967176	26.672513154597844
34-35	22.86394387371586	21.911801553495362	33.525432222500626	21.69882235028815
36-37	21.986970684039086	23.05186670007517	24.843397644700577	30.117764971185167
38-39	22.738661989476324	29.2658481583563	26.509646705086443	21.48584314708093
40-41	22.70107742420446	24.981207717364068	28.288649461287896	24.029065397143572
42-43	21.348033074417437	21.109997494362315	33.36256577298923	24.17940365823102
44-45	19.305938361312954	30.957153595590075	26.48459032823854	23.25231771485843
46-47	23.23978952643448	27.424204460035078	23.415184164369833	25.920821849160614
48-49	19.19318466549737	30.017539463793536	22.90152843898772	27.887747431721372
50-51	21.122525682786268	23.25231771485843	32.410423452768725	23.214733149586568
52-53	19.54397394136808	27.44926083688299	28.564269606614882	24.442495615134053
54-55	18.00300676522175	21.824104234527688	34.85342019543974	25.319468804810825
56-57	24.40491104986219	20.54622901528439	34.41493360060136	20.633926334252067
58-59	18.12828864946129	24.693059383613132	35.166624906038585	22.012027060886997
60-61	24.705587572037082	29.2658481583563	28.752192432974194	17.276371836632425
62-63	18.291155098972688	22.362816336757703	35.07892758707091	24.267100977198698
64-65	18.692057128539215	21.197694813329992	27.93786018541719	32.17238787271361
66-67	18.529190679027813	21.648709596592333	36.67000751691305	23.1520922074668
68-69	19.19318466549737	24.605362064645455	27.286394387371587	28.915058882485596
70-71	18.078175895765472	25.068905036331746	28.46404409922325	28.388874968679527
72-73	21.26033575544976	24.31721373089451	23.001753946379353	31.420696567276373
74-75	23.08945126534703	26.096216487095962	25.36958155850664	25.444750689050366
76-77	27.950388373841147	23.653219744424955	26.033575544976195	22.362816336757703
78-79	23.62816336757705	25.457278877474316	31.445752944124276	19.468804810824356
80-81	26.096216487095962	22.964169381107492	32.67351540967176	18.26609872212478
82-83	21.084941117514408	23.72838887496868	28.702079679278373	26.48459032823854
84-85	23.891255324480078	28.313705838135807	26.797795038837386	20.99724379854673
86-87	21.723878727136057	26.158857429215736	29.616637434227012	22.500626409421198
88-89	18.403908794788272	34.70308193435229	26.146329240791783	20.74668003006765
90-91	20.170383362565772	32.410423452768725	18.190929591581057	29.228263593084442
92-93	21.811576046103735	32.36031069907291	23.50288148333751	22.325231771485843
94-95	27.61212728639439	28.73966424455024	23.916311701327988	19.731896767727385
96-97	31.77148584314708	28.276121272863946	17.890253069406164	22.062139814582814
98-99	28.113254823352545	22.776246554748184	19.230769230769234	29.879729391130045
100-101	20.120345994734862	26.96502444528018	25.42309138773975	27.491538172245207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.0
33	1.0
34	1.0
35	4.5
36	8.5
37	7.5
38	5.0
39	15.0
40	46.0
41	93.5
42	76.0
43	61.5
44	180.0
45	414.5
46	473.0
47	591.5
48	744.0
49	503.5
50	271.5
51	221.0
52	186.0
53	80.5
54	2.5
55	0.5
56	1.0
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17539463793535454
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.06264094211976949
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.560010022550738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.48792270531401	8.945126534703082
2	9.178743961352657	2.8564269606614885
3	5.958132045088567	2.781257830117765
4	5.152979066022544	3.2072162365321977
5	2.4154589371980677	1.8792282635930844
6	2.254428341384863	2.1047356552242547
7	2.254428341384863	2.4555249310949634
8	0.966183574879227	1.202706088699574
9	0.4830917874396135	0.6765221748935104
>10	10.950080515297907	37.88524179403658
>50	2.254428341384863	23.903783512904035
>100	0.644122383252818	12.102230017539464
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCA	158	3.9589075419694315	No Hit
CTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCAT	112	2.8063142069656726	No Hit
CTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGG	108	2.7060886995740416	No Hit
CCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGT	105	2.6309195690303184	No Hit
GTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAAC	100	2.505637684790779	No Hit
GCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATC	99	2.480581307942871	No Hit
GCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAG	92	2.305186670007517	No Hit
GCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGT	79	1.9794537709847158	No Hit
CCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTA	68	1.7038336256577298	No Hit
GGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGAT	67	1.678777248809822	No Hit
GCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAA	62	1.5534953645702831	No Hit
CCTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAA	61	1.5284389877223752	No Hit
CTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCT	60	1.5033826108744675	No Hit
CGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTAC	56	1.4031571034828363	No Hit
CAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCAT	53	1.327987972939113	No Hit
GTCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAG	53	1.327987972939113	No Hit
GCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACT	52	1.3029315960912053	No Hit
GTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCT	52	1.3029315960912053	No Hit
CTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTG	43	1.077424204460035	No Hit
CTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGC	43	1.077424204460035	No Hit
GTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTC	41	1.0273114507642196	No Hit
GTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAG	40	1.0022550739163119	No Hit
GTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCA	39	0.9771986970684038	No Hit
CCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCA	39	0.9771986970684038	No Hit
AGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	37	0.9270859433725883	No Hit
CTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAAT	36	0.9020295665246805	No Hit
CCCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAA	36	0.9020295665246805	No Hit
GGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCG	35	0.8769731896767727	No Hit
GTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGAT	35	0.8769731896767727	No Hit
TGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGA	35	0.8769731896767727	No Hit
CTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCA	33	0.8268604359809572	No Hit
GCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCC	33	0.8268604359809572	No Hit
CCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGA	32	0.8018040591330494	No Hit
CCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCT	31	0.7767476822851416	No Hit
ATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGT	31	0.7767476822851416	No Hit
CGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGC	30	0.7516913054372337	No Hit
CTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAA	29	0.726634928589326	No Hit
CACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTC	28	0.7015785517414181	No Hit
CCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTG	28	0.7015785517414181	No Hit
CCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCC	27	0.6765221748935104	No Hit
CCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCT	27	0.6765221748935104	No Hit
CGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACC	26	0.6514657980456027	No Hit
GCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAG	25	0.6264094211976947	No Hit
GCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCAC	25	0.6264094211976947	No Hit
CCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTA	24	0.601353044349787	No Hit
GTTGTCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCA	24	0.601353044349787	No Hit
GCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAG	23	0.5762966675018792	No Hit
CGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAAC	23	0.5762966675018792	No Hit
CACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTG	22	0.5512402906539714	No Hit
GTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCT	22	0.5512402906539714	No Hit
CCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCAC	21	0.5261839138060637	No Hit
CGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTT	20	0.5011275369581559	No Hit
TAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCT	20	0.5011275369581559	No Hit
CATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTC	19	0.476071160110248	No Hit
CGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACA	18	0.45101478326234024	No Hit
GCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAG	18	0.45101478326234024	No Hit
TTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGA	18	0.45101478326234024	No Hit
GCCAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGG	17	0.42595840641443244	No Hit
TGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACC	16	0.4009020295665247	No Hit
GCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCT	16	0.4009020295665247	No Hit
CTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACT	16	0.4009020295665247	No Hit
CTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGA	16	0.4009020295665247	No Hit
CTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGT	15	0.37584565271861686	No Hit
GGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAA	15	0.37584565271861686	No Hit
CTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTC	15	0.37584565271861686	No Hit
CGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAA	14	0.35078927587070907	No Hit
GGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGT	14	0.35078927587070907	No Hit
TGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTT	14	0.35078927587070907	No Hit
CTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTG	13	0.32573289902280134	No Hit
CAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGA	13	0.32573289902280134	No Hit
ATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATT	13	0.32573289902280134	No Hit
TGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGT	13	0.32573289902280134	No Hit
GTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCT	12	0.3006765221748935	No Hit
ATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTG	12	0.3006765221748935	No Hit
GGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTG	12	0.3006765221748935	No Hit
ATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATC	12	0.3006765221748935	No Hit
CCAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGT	11	0.2756201453269857	No Hit
ACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCG	11	0.2756201453269857	No Hit
TTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCAT	11	0.2756201453269857	No Hit
GTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAA	11	0.2756201453269857	No Hit
CAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTG	11	0.2756201453269857	No Hit
GTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCT	11	0.2756201453269857	No Hit
GGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGA	11	0.2756201453269857	No Hit
GGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTA	11	0.2756201453269857	No Hit
CCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTT	10	0.25056376847907796	No Hit
CCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTG	10	0.25056376847907796	No Hit
TGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCT	9	0.22550739163117012	No Hit
CCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTT	9	0.22550739163117012	No Hit
CTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCA	9	0.22550739163117012	No Hit
GACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGT	8	0.20045101478326235	No Hit
TGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAA	8	0.20045101478326235	No Hit
CTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGAATTAGATCCTCC	8	0.20045101478326235	No Hit
ACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCA	8	0.20045101478326235	No Hit
GCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACA	8	0.20045101478326235	No Hit
GCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCA	8	0.20045101478326235	No Hit
TGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	7	0.17539463793535454	No Hit
AGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGC	7	0.17539463793535454	No Hit
CTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTG	7	0.17539463793535454	No Hit
ATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCC	7	0.17539463793535454	No Hit
CTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTC	7	0.17539463793535454	No Hit
CGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	7	0.17539463793535454	No Hit
GCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCC	7	0.17539463793535454	No Hit
TTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCAT	7	0.17539463793535454	No Hit
TATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATC	7	0.17539463793535454	No Hit
GGGTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCC	7	0.17539463793535454	No Hit
CCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAA	7	0.17539463793535454	No Hit
TGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATG	7	0.17539463793535454	No Hit
ATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTT	7	0.17539463793535454	No Hit
GTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTC	7	0.17539463793535454	No Hit
CGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAA	6	0.15033826108744675	No Hit
CCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGGGA	6	0.15033826108744675	No Hit
GACCGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGAT	6	0.15033826108744675	No Hit
TCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGT	6	0.15033826108744675	No Hit
CCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGG	6	0.15033826108744675	No Hit
AGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATG	6	0.15033826108744675	No Hit
CATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTAT	6	0.15033826108744675	No Hit
ATCCTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGG	6	0.15033826108744675	No Hit
GAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCT	6	0.15033826108744675	No Hit
TGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTT	6	0.15033826108744675	No Hit
CCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACC	6	0.15033826108744675	No Hit
GGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGA	6	0.15033826108744675	No Hit
TGTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGA	6	0.15033826108744675	No Hit
CCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTG	6	0.15033826108744675	No Hit
CCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTT	5	0.12528188423953898	No Hit
TCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTC	5	0.12528188423953898	No Hit
TCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTT	5	0.12528188423953898	No Hit
GTCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATT	5	0.12528188423953898	No Hit
GCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGAATTAGATCCTC	5	0.12528188423953898	No Hit
GGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	5	0.12528188423953898	No Hit
CAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGA	5	0.12528188423953898	No Hit
TTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAAT	5	0.12528188423953898	No Hit
GTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAG	5	0.12528188423953898	No Hit
TGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATT	5	0.12528188423953898	No Hit
TGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTT	5	0.12528188423953898	No Hit
CTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTAT	5	0.12528188423953898	No Hit
CTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACAC	5	0.12528188423953898	No Hit
ATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTT	5	0.12528188423953898	No Hit
GGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAA	5	0.12528188423953898	No Hit
TTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAA	4	0.10022550739163118	No Hit
GGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAG	4	0.10022550739163118	No Hit
GTCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTT	4	0.10022550739163118	No Hit
CTGTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAG	4	0.10022550739163118	No Hit
GGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCC	4	0.10022550739163118	No Hit
CCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGTCTAATAA	4	0.10022550739163118	No Hit
AGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGAT	4	0.10022550739163118	No Hit
CTCATGAATTAGATCCTCCATGCAGATGATGCCAAACTTACCAAGAGACC	4	0.10022550739163118	No Hit
GATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCC	4	0.10022550739163118	No Hit
GGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTT	4	0.10022550739163118	No Hit
CTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACCTCGTGGG	4	0.10022550739163118	No Hit
AATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTA	4	0.10022550739163118	No Hit
CCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGT	4	0.10022550739163118	No Hit
AAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAG	4	0.10022550739163118	No Hit
CTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTG	4	0.10022550739163118	No Hit
GAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCA	4	0.10022550739163118	No Hit
TTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGT	4	0.10022550739163118	No Hit
AGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTAT	4	0.10022550739163118	No Hit
CTGGCTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGC	4	0.10022550739163118	No Hit
GGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAA	4	0.10022550739163118	No Hit
GCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAG	4	0.10022550739163118	No Hit
TATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAACTTACCA	4	0.10022550739163118	No Hit
CCCGGCTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAA	4	0.10022550739163118	No Hit
TATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAAC	4	0.10022550739163118	No Hit
GATCCTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAG	4	0.10022550739163118	No Hit
GTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAA	4	0.10022550739163118	No Hit
GAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTG	4	0.10022550739163118	No Hit
CATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTT	4	0.10022550739163118	No Hit
CCGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTT	4	0.10022550739163118	No Hit
TCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTG	4	0.10022550739163118	No Hit
CCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTC	4	0.10022550739163118	No Hit
GCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTG	4	0.10022550739163118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.02505637684790779	0.0	0.0	0.0	0.0
82-83	0.2630919569030318	0.0	0.0	0.0	0.0
84-85	0.8644450012528189	0.0	0.0	0.0	0.0
86-87	1.566023552994237	0.0	0.0	0.0	0.0
88-89	2.530694061638687	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25558502 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558502_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3990
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6578947368421	33.0	33.0	34.0	32.0	34.0
2	32.77418546365915	33.0	33.0	34.0	32.0	34.0
3	32.827568922305765	34.0	33.0	34.0	32.0	34.0
4	32.78796992481203	34.0	33.0	34.0	32.0	34.0
5	32.74360902255639	34.0	33.0	34.0	32.0	34.0
6	36.83182957393484	38.0	38.0	38.0	35.0	38.0
7	36.871929824561406	38.0	38.0	38.0	36.0	38.0
8	36.83383458646617	38.0	38.0	38.0	36.0	38.0
9	36.868671679197995	38.0	38.0	38.0	36.0	38.0
10-11	36.74085213032582	38.0	38.0	38.0	35.0	38.0
12-13	36.81503759398497	38.0	38.0	38.0	35.0	38.0
14-15	36.835087719298244	38.0	38.0	38.0	36.0	38.0
16-17	36.835338345864656	38.0	38.0	38.0	35.5	38.0
18-19	36.74749373433584	38.0	38.0	38.0	35.0	38.0
20-21	36.71478696741855	38.0	38.0	38.0	35.0	38.0
22-23	36.696365914786966	38.0	38.0	38.0	35.0	38.0
24-25	36.631077694235586	38.0	38.0	38.0	35.0	38.0
26-27	36.68383458646616	38.0	38.0	38.0	35.0	38.0
28-29	36.70012531328321	38.0	38.0	38.0	35.0	38.0
30-31	36.71704260651629	38.0	38.0	38.0	35.0	38.0
32-33	36.694736842105264	38.0	38.0	38.0	35.0	38.0
34-35	36.70676691729324	38.0	38.0	38.0	35.0	38.0
36-37	36.711779448621556	38.0	38.0	38.0	35.5	38.0
38-39	36.73984962406015	38.0	38.0	38.0	35.0	38.0
40-41	36.61466165413533	38.0	38.0	38.0	35.0	38.0
42-43	36.644235588972435	38.0	38.0	38.0	35.0	38.0
44-45	36.634586466165416	38.0	38.0	38.0	35.0	38.0
46-47	36.56177944862155	38.0	38.0	38.0	34.5	38.0
48-49	36.62092731829574	38.0	38.0	38.0	34.5	38.0
50-51	36.692606516290724	38.0	38.0	38.0	35.0	38.0
52-53	36.54298245614035	38.0	38.0	38.0	34.0	38.0
54-55	36.58170426065163	38.0	38.0	38.0	35.0	38.0
56-57	36.527067669172936	38.0	38.0	38.0	34.5	38.0
58-59	36.55902255639098	38.0	38.0	38.0	34.5	38.0
60-61	36.52894736842106	38.0	38.0	38.0	34.0	38.0
62-63	36.540100250626566	38.0	38.0	38.0	34.0	38.0
64-65	36.48922305764411	38.0	38.0	38.0	34.0	38.0
66-67	36.50050125313283	38.0	38.0	38.0	34.0	38.0
68-69	36.49010025062657	38.0	38.0	38.0	34.5	38.0
70-71	36.413784461152886	38.0	38.0	38.0	34.0	38.0
72-73	36.50476190476191	38.0	38.0	38.0	34.0	38.0
74-75	36.31917293233083	38.0	38.0	38.0	33.5	38.0
76-77	36.37731829573935	38.0	38.0	38.0	34.0	38.0
78-79	36.45639097744361	38.0	38.0	38.0	34.0	38.0
80-81	36.32481203007519	38.0	38.0	38.0	34.0	38.0
82-83	36.28809523809524	38.0	38.0	38.0	34.0	38.0
84-85	36.267042606516284	38.0	38.0	38.0	34.0	38.0
86-87	36.201879699248124	38.0	38.0	38.0	34.0	38.0
88-89	36.292606516290725	38.0	38.0	38.0	34.0	38.0
90-91	36.120551378446116	38.0	38.0	38.0	33.0	38.0
92-93	36.13020050125313	38.0	38.0	38.0	33.0	38.0
94-95	36.109649122807014	38.0	38.0	38.0	33.5	38.0
96-97	36.09874686716792	38.0	38.0	38.0	33.5	38.0
98-99	35.970426065162904	38.0	38.0	38.0	33.5	38.0
100-101	34.8750626566416	38.0	36.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	3.0
4	0.0
5	1.0
6	1.0
7	2.0
8	1.0
9	1.0
10	1.0
11	0.0
12	0.0
13	2.0
14	1.0
15	0.0
16	1.0
17	2.0
18	2.0
19	4.0
20	6.0
21	3.0
22	9.0
23	7.0
24	13.0
25	12.0
26	19.0
27	35.0
28	42.0
29	37.0
30	41.0
31	60.0
32	73.0
33	96.0
34	111.0
35	179.0
36	387.0
37	2834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.270676691729324	17.318295739348372	9.172932330827068	45.23809523809524
2	21.854636591478695	24.636591478696744	32.280701754385966	21.228070175438596
3	15.964912280701753	26.741854636591476	33.959899749373434	23.333333333333332
4	20.100250626566414	35.6390977443609	19.172932330827066	25.087719298245613
5	25.36340852130326	41.904761904761905	17.017543859649123	15.714285714285714
6	21.228070175438596	41.228070175438596	19.072681704260653	18.471177944862156
7	23.684210526315788	19.523809523809526	35.6390977443609	21.152882205513784
8	22.606516290726816	23.583959899749374	30.6516290726817	23.157894736842106
9	25.062656641604008	25.739348370927317	27.869674185463662	21.328320802005013
10-11	24.862155388471177	33.94736842105263	18.784461152882205	22.406015037593985
12-13	23.145363408521302	23.032581453634084	26.704260651629074	27.11779448621554
14-15	22.41854636591479	29.82456140350877	26.71679197994987	21.040100250626566
16-17	24.899749373433583	26.804511278195488	22.45614035087719	25.839598997493734
18-19	22.807017543859647	28.809523809523807	28.345864661654137	20.037593984962406
20-21	26.666666666666668	27.11779448621554	23.834586466165415	22.380952380952383
22-23	26.94235588972431	28.89724310776942	22.518796992481203	21.641604010025063
24-25	25.36340852130326	24.29824561403509	27.368421052631582	22.969924812030076
26-27	23.32080200501253	28.87218045112782	25.062656641604008	22.74436090225564
28-29	25.98997493734336	26.71679197994987	23.94736842105263	23.345864661654137
30-31	22.706766917293233	28.471177944862152	23.49624060150376	25.32581453634085
32-33	22.167919799498748	31.854636591478698	24.32330827067669	21.654135338345863
34-35	23.583959899749374	27.21804511278195	26.14035087719298	23.05764411027569
36-37	22.55639097744361	28.884711779448622	25.914786967418546	22.644110275689222
38-39	24.786967418546364	29.28571428571429	25.81453634085213	20.112781954887218
40-41	24.82456140350877	30.213032581453636	22.305764411027567	22.656641604010026
42-43	22.74436090225564	28.7468671679198	25.087719298245613	23.42105263157895
44-45	22.55639097744361	29.160401002506266	28.195488721804512	20.087719298245617
46-47	26.57894736842105	24.285714285714285	23.809523809523807	25.32581453634085
48-49	22.105263157894736	29.586466165413533	25.67669172932331	22.63157894736842
50-51	24.235588972431078	29.624060150375943	23.170426065162907	22.969924812030076
52-53	26.027568922305765	26.741854636591476	25.6390977443609	21.591478696741856
54-55	23.533834586466167	27.60651629072682	27.04260651629073	21.81704260651629
56-57	21.904761904761905	29.912280701754383	23.79699248120301	24.385964912280702
58-59	26.81704260651629	25.225563909774433	28.233082706766915	19.724310776942357
60-61	27.330827067669173	30.526315789473685	23.521303258145362	18.62155388471178
62-63	27.268170426065165	25.75187969924812	25.664160401002505	21.31578947368421
64-65	25.88972431077694	28.78446115288221	24.736842105263158	20.588972431077696
66-67	24.32330827067669	28.533834586466167	24.24812030075188	22.894736842105264
68-69	22.218045112781954	26.71679197994987	29.06015037593985	22.005012531328322
70-71	25.012531328320804	29.82456140350877	24.01002506265664	21.152882205513784
72-73	22.61904761904762	26.71679197994987	24.962406015037594	25.701754385964914
74-75	24.097744360902258	29.411027568922304	25.902255639097742	20.588972431077696
76-77	25.288220551378448	29.28571428571429	20.588972431077696	24.837092731829575
78-79	24.93734335839599	29.348370927318296	26.44110275689223	19.273182957393484
80-81	20.98997493734336	32.042606516290725	24.260651629072683	22.706766917293233
82-83	24.085213032581454	29.29824561403509	23.370927318295738	23.24561403508772
84-85	21.641604010025063	26.842105263157894	27.06766917293233	24.44862155388471
86-87	23.32080200501253	32.631578947368425	22.75689223057644	21.290726817042607
88-89	24.32330827067669	28.208020050125317	24.74937343358396	22.719298245614038
90-91	28.132832080200505	25.85213032581454	27.456140350877195	18.55889724310777
92-93	23.107769423558896	32.24310776942356	23.32080200501253	21.328320802005013
94-95	25.827067669172934	28.095238095238095	23.1328320802005	22.94486215538847
96-97	28.60902255639098	29.097744360902254	21.403508771929825	20.88972431077694
98-99	24.29824561403509	31.04010025062657	26.378446115288224	18.283208020050125
100-101	24.51127819548872	26.39097744360902	27.017543859649123	22.080200501253135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	1.0
34	3.5
35	6.0
36	8.0
37	7.0
38	6.0
39	11.5
40	22.0
41	23.0
42	74.5
43	212.5
44	300.5
45	382.0
46	579.5
47	738.5
48	522.0
49	321.0
50	305.0
51	191.5
52	111.5
53	76.5
54	54.0
55	22.0
56	7.0
57	1.5
58	0.5
59	0.0
60	0.0
61	1.0
62	1.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.1203007518797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.983402489626556	7.969924812030076
2	12.171507607192254	4.4110275689223055
3	7.883817427385892	4.285714285714286
4	5.94744121715076	4.31077694235589
5	4.011065006915629	3.634085213032581
6	2.351313969571231	2.556390977443609
7	3.18118948824343	4.035087719298246
8	2.4896265560165975	3.6090225563909777
9	2.4896265560165975	4.06015037593985
>10	14.661134163208853	50.30075187969925
>50	0.6915629322268326	8.170426065162907
>100	0.13831258644536654	2.656641604010025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGC	106	2.656641604010025	No Hit
CTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACT	84	2.1052631578947367	No Hit
CCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCA	76	1.9047619047619049	No Hit
CCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAA	59	1.4786967418546366	No Hit
CAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGC	56	1.4035087719298245	No Hit
CTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAG	51	1.2781954887218046	No Hit
GGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGC	47	1.1779448621553885	No Hit
CTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGC	47	1.1779448621553885	No Hit
GCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTG	47	1.1779448621553885	No Hit
CCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAA	42	1.0526315789473684	No Hit
GTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTTTGTAG	41	1.0275689223057645	No Hit
CTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCAC	38	0.9523809523809524	No Hit
GGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCT	38	0.9523809523809524	No Hit
CGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAG	35	0.8771929824561403	No Hit
CTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTAC	33	0.8270676691729323	No Hit
CCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCC	33	0.8270676691729323	No Hit
CCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCA	32	0.8020050125313283	No Hit
CAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAG	31	0.7769423558897243	No Hit
CAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCA	30	0.7518796992481203	No Hit
CCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAA	30	0.7518796992481203	No Hit
CAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACAT	30	0.7518796992481203	No Hit
CCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGAT	28	0.7017543859649122	No Hit
CAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGG	28	0.7017543859649122	No Hit
CAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTC	27	0.6766917293233082	No Hit
CTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAG	27	0.6766917293233082	No Hit
AATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCG	25	0.6265664160401002	No Hit
CGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTT	25	0.6265664160401002	No Hit
GGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATAC	25	0.6265664160401002	No Hit
GTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGAT	24	0.6015037593984963	No Hit
CACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGG	24	0.6015037593984963	No Hit
GTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTT	23	0.5764411027568922	No Hit
ATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAAT	23	0.5764411027568922	No Hit
CTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATC	22	0.5513784461152882	No Hit
CTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTA	22	0.5513784461152882	No Hit
GCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTCA	22	0.5513784461152882	No Hit
AACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCC	22	0.5513784461152882	No Hit
GTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTT	21	0.5263157894736842	No Hit
CTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATC	21	0.5263157894736842	No Hit
GCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCT	21	0.5263157894736842	No Hit
CGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGA	20	0.5012531328320802	No Hit
GCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGT	20	0.5012531328320802	No Hit
GCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCA	19	0.4761904761904762	No Hit
CCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAG	19	0.4761904761904762	No Hit
GCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGG	19	0.4761904761904762	No Hit
GTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCG	19	0.4761904761904762	No Hit
ATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTG	19	0.4761904761904762	No Hit
GTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACA	19	0.4761904761904762	No Hit
GAAAAAGACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAG	18	0.4511278195488722	No Hit
AGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATT	18	0.4511278195488722	No Hit
CAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAAT	18	0.4511278195488722	No Hit
CAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTC	18	0.4511278195488722	No Hit
CGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTG	18	0.4511278195488722	No Hit
GGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCA	17	0.4260651629072682	No Hit
ATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAG	17	0.4260651629072682	No Hit
GATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCAT	17	0.4260651629072682	No Hit
CCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATC	17	0.4260651629072682	No Hit
GCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCA	17	0.4260651629072682	No Hit
CAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTA	17	0.4260651629072682	No Hit
TATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTC	17	0.4260651629072682	No Hit
AGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAG	16	0.40100250626566414	No Hit
CTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCA	16	0.40100250626566414	No Hit
TTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCG	16	0.40100250626566414	No Hit
GGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTC	16	0.40100250626566414	No Hit
GTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGA	16	0.40100250626566414	No Hit
GCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCAT	16	0.40100250626566414	No Hit
GGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAG	15	0.37593984962406013	No Hit
CGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCT	15	0.37593984962406013	No Hit
CATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCT	15	0.37593984962406013	No Hit
CCCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCA	15	0.37593984962406013	No Hit
ATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGA	15	0.37593984962406013	No Hit
ACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCC	14	0.3508771929824561	No Hit
AGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAAT	14	0.3508771929824561	No Hit
TGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATA	14	0.3508771929824561	No Hit
GGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGT	14	0.3508771929824561	No Hit
GGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACA	13	0.3258145363408521	No Hit
CTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAAT	13	0.3258145363408521	No Hit
CATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGA	13	0.3258145363408521	No Hit
CTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAA	13	0.3258145363408521	No Hit
CTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGC	13	0.3258145363408521	No Hit
GCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACA	13	0.3258145363408521	No Hit
GTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAA	13	0.3258145363408521	No Hit
CGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCC	13	0.3258145363408521	No Hit
CTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAA	12	0.30075187969924816	No Hit
CCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAG	12	0.30075187969924816	No Hit
CTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTA	12	0.30075187969924816	No Hit
GGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCT	12	0.30075187969924816	No Hit
AATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCA	12	0.30075187969924816	No Hit
TGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACG	12	0.30075187969924816	No Hit
ATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAA	12	0.30075187969924816	No Hit
AGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCC	12	0.30075187969924816	No Hit
GAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCT	12	0.30075187969924816	No Hit
GGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTA	11	0.2756892230576441	No Hit
AATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGA	11	0.2756892230576441	No Hit
ATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGG	11	0.2756892230576441	No Hit
CAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTG	11	0.2756892230576441	No Hit
GAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAG	11	0.2756892230576441	No Hit
CATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAAC	11	0.2756892230576441	No Hit
TTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGG	11	0.2756892230576441	No Hit
CAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGC	11	0.2756892230576441	No Hit
CAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATG	11	0.2756892230576441	No Hit
ATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTC	11	0.2756892230576441	No Hit
CTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGA	11	0.2756892230576441	No Hit
TGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTG	10	0.2506265664160401	No Hit
GTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGA	10	0.2506265664160401	No Hit
TGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGAC	10	0.2506265664160401	No Hit
GTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCAC	10	0.2506265664160401	No Hit
CTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGA	10	0.2506265664160401	No Hit
AGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGA	10	0.2506265664160401	No Hit
AAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTC	10	0.2506265664160401	No Hit
CAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAA	10	0.2506265664160401	No Hit
TTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTT	10	0.2506265664160401	No Hit
GTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGA	10	0.2506265664160401	No Hit
AGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCA	9	0.2255639097744361	No Hit
GAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAG	9	0.2255639097744361	No Hit
TAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTG	9	0.2255639097744361	No Hit
GTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATA	9	0.2255639097744361	No Hit
GGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAAT	9	0.2255639097744361	No Hit
GCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAA	9	0.2255639097744361	No Hit
TCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATA	9	0.2255639097744361	No Hit
CAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTC	9	0.2255639097744361	No Hit
AGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCT	9	0.2255639097744361	No Hit
GGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAA	9	0.2255639097744361	No Hit
CGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTAT	9	0.2255639097744361	No Hit
CTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCT	9	0.2255639097744361	No Hit
GACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAAC	9	0.2255639097744361	No Hit
TCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGG	9	0.2255639097744361	No Hit
ATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAA	9	0.2255639097744361	No Hit
CTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGT	9	0.2255639097744361	No Hit
AAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGA	9	0.2255639097744361	No Hit
CTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAA	9	0.2255639097744361	No Hit
GGTGGAGATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACG	8	0.20050125313283207	No Hit
CCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCT	8	0.20050125313283207	No Hit
AGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGT	8	0.20050125313283207	No Hit
GGGTACCCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTA	8	0.20050125313283207	No Hit
AAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGAT	8	0.20050125313283207	No Hit
ACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATT	8	0.20050125313283207	No Hit
CGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTG	8	0.20050125313283207	No Hit
CTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTTTGTAGAAGGT	8	0.20050125313283207	No Hit
TGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAG	8	0.20050125313283207	No Hit
CAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGA	8	0.20050125313283207	No Hit
AAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATG	8	0.20050125313283207	No Hit
CGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGA	8	0.20050125313283207	No Hit
ATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCT	8	0.20050125313283207	No Hit
CTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCA	8	0.20050125313283207	No Hit
AGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATG	8	0.20050125313283207	No Hit
CAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAG	8	0.20050125313283207	No Hit
GGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTG	8	0.20050125313283207	No Hit
GAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTA	8	0.20050125313283207	No Hit
CTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTT	7	0.17543859649122806	No Hit
ATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTT	7	0.17543859649122806	No Hit
TTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAAC	7	0.17543859649122806	No Hit
ATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAG	7	0.17543859649122806	No Hit
GAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTT	7	0.17543859649122806	No Hit
GCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGT	7	0.17543859649122806	No Hit
CCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAA	7	0.17543859649122806	No Hit
AAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGC	7	0.17543859649122806	No Hit
CAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGG	7	0.17543859649122806	No Hit
AAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGA	7	0.17543859649122806	No Hit
TATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTT	7	0.17543859649122806	No Hit
CAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCC	7	0.17543859649122806	No Hit
ATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTC	7	0.17543859649122806	No Hit
CTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGC	7	0.17543859649122806	No Hit
ACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTC	7	0.17543859649122806	No Hit
CTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTG	7	0.17543859649122806	No Hit
AACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGAT	7	0.17543859649122806	No Hit
GGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTC	7	0.17543859649122806	No Hit
CTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAGACCAGATAAAC	7	0.17543859649122806	No Hit
AAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGT	7	0.17543859649122806	No Hit
AGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAG	7	0.17543859649122806	No Hit
GGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTG	7	0.17543859649122806	No Hit
CTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCA	7	0.17543859649122806	No Hit
ATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGC	6	0.15037593984962408	No Hit
TCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCT	6	0.15037593984962408	No Hit
CCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGG	6	0.15037593984962408	No Hit
GTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGT	6	0.15037593984962408	No Hit
AGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTC	6	0.15037593984962408	No Hit
GATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGC	6	0.15037593984962408	No Hit
AGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCA	6	0.15037593984962408	No Hit
GCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTG	6	0.15037593984962408	No Hit
ATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCT	6	0.15037593984962408	No Hit
AACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGC	6	0.15037593984962408	No Hit
CAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTC	6	0.15037593984962408	No Hit
GGTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAA	6	0.15037593984962408	No Hit
GGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGC	6	0.15037593984962408	No Hit
AGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTT	6	0.15037593984962408	No Hit
TGGAGATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACGGA	6	0.15037593984962408	No Hit
TCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCC	6	0.15037593984962408	No Hit
TAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACC	6	0.15037593984962408	No Hit
GGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGG	5	0.12531328320802004	No Hit
CAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTT	5	0.12531328320802004	No Hit
GTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACT	5	0.12531328320802004	No Hit
GAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGG	5	0.12531328320802004	No Hit
AATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACT	5	0.12531328320802004	No Hit
ATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTT	5	0.12531328320802004	No Hit
CCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCAC	5	0.12531328320802004	No Hit
TGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGG	5	0.12531328320802004	No Hit
CAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCAT	5	0.12531328320802004	No Hit
GCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTT	5	0.12531328320802004	No Hit
GACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAGACCAGA	5	0.12531328320802004	No Hit
AAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAA	5	0.12531328320802004	No Hit
GCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAG	5	0.12531328320802004	No Hit
GCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAA	5	0.12531328320802004	No Hit
TCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGT	5	0.12531328320802004	No Hit
CAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGG	5	0.12531328320802004	No Hit
GATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAA	5	0.12531328320802004	No Hit
GGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTT	5	0.12531328320802004	No Hit
GGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACT	5	0.12531328320802004	No Hit
ATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCC	5	0.12531328320802004	No Hit
AAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAA	5	0.12531328320802004	No Hit
CGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTA	5	0.12531328320802004	No Hit
CACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTAT	5	0.12531328320802004	No Hit
CGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAA	5	0.12531328320802004	No Hit
AGACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAGACCAG	5	0.12531328320802004	No Hit
GAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGC	5	0.12531328320802004	No Hit
GCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTA	5	0.12531328320802004	No Hit
GCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGAT	5	0.12531328320802004	No Hit
GGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATC	5	0.12531328320802004	No Hit
AACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAA	4	0.10025062656641603	No Hit
AACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATG	4	0.10025062656641603	No Hit
GCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCA	4	0.10025062656641603	No Hit
TGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGA	4	0.10025062656641603	No Hit
CAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGG	4	0.10025062656641603	No Hit
TAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGA	4	0.10025062656641603	No Hit
GATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAAC	4	0.10025062656641603	No Hit
GTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCA	4	0.10025062656641603	No Hit
TTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACG	4	0.10025062656641603	No Hit
AGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAA	4	0.10025062656641603	No Hit
AGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGT	4	0.10025062656641603	No Hit
AAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCG	4	0.10025062656641603	No Hit
CTATTTCTGGCAATCGCAAAACTATGTGTAGACTCACCTAGTTTTGAATT	4	0.10025062656641603	No Hit
GACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCT	4	0.10025062656641603	No Hit
TGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCC	4	0.10025062656641603	No Hit
GAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTG	4	0.10025062656641603	No Hit
GATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACGGATGAA	4	0.10025062656641603	No Hit
AGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCA	4	0.10025062656641603	No Hit
CTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTA	4	0.10025062656641603	No Hit
TTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCT	4	0.10025062656641603	No Hit
GGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATC	4	0.10025062656641603	No Hit
TGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAAC	4	0.10025062656641603	No Hit
ATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCT	4	0.10025062656641603	No Hit
AGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCA	4	0.10025062656641603	No Hit
GCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTG	4	0.10025062656641603	No Hit
AATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGT	4	0.10025062656641603	No Hit
TGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGT	4	0.10025062656641603	No Hit
CTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTC	4	0.10025062656641603	No Hit
CAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGC	4	0.10025062656641603	No Hit
GGGATGAAGAAAAAGACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAA	4	0.10025062656641603	No Hit
TTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGG	4	0.10025062656641603	No Hit
GGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAG	4	0.10025062656641603	No Hit
TCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTG	4	0.10025062656641603	No Hit
AGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGC	4	0.10025062656641603	No Hit
GCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATG	4	0.10025062656641603	No Hit
ACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGA	4	0.10025062656641603	No Hit
GTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGT	4	0.10025062656641603	No Hit
AGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATT	4	0.10025062656641603	No Hit
GATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAA	4	0.10025062656641603	No Hit
TCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTC	4	0.10025062656641603	No Hit
CCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGG	4	0.10025062656641603	No Hit
AAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTG	4	0.10025062656641603	No Hit
CAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAG	4	0.10025062656641603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.02506265664160401	0.0	0.0	0.0	0.0
58-59	0.02506265664160401	0.0	0.0	0.0	0.0
60-61	0.02506265664160401	0.0	0.0	0.0	0.0
62-63	0.02506265664160401	0.0	0.0	0.0	0.0
64-65	0.02506265664160401	0.0	0.0	0.0	0.0
66-67	0.02506265664160401	0.0	0.0	0.0	0.0
68-69	0.02506265664160401	0.0	0.0	0.0	0.0
70-71	0.02506265664160401	0.0	0.0	0.0	0.0
72-73	0.02506265664160401	0.0	0.0	0.0	0.0
74-75	0.02506265664160401	0.0	0.0	0.0	0.0
76-77	0.02506265664160401	0.0	0.0	0.0	0.0
78-79	0.02506265664160401	0.0	0.0	0.0	0.0
80-81	0.05012531328320802	0.0	0.0	0.0	0.0
82-83	0.3007518796992481	0.0	0.0	0.0	0.0
84-85	0.9147869674185464	0.0	0.0	0.0	0.0
86-87	1.5914786967418546	0.0	0.0	0.0	0.0
88-89	2.5438596491228074	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 5 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 5 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 11 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 14 READS because READLEN < 1
Read 583 spots for SRR25558502.sra
Written 583 spots for SRR25558502.sra
Rejected 1 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 6 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 1 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 1 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 4 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 5 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 10 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 5 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 3 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 1 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
Rejected 3 READS because READLEN < 1
Read 578 spots for SRR25558502.sra
Written 578 spots for SRR25558502.sra
SRR ids: ['SRR25558502.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_glu55cv4
SRR25558502.sra spots: 9253
blocks: [[1, 578], [579, 1156], [1157, 1734], [1735, 2312], [2313, 2890], [2891, 3468], [3469, 4046], [4047, 4624], [4625, 5202], [5203, 5780], [5781, 6358], [6359, 6936], [6937, 7514], [7515, 8092], [8093, 8670], [8671, 9253]]
SRR25558502 file size 1
SRR25558502 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25558502 SRR25558502_1.fastq SRR25558502_2.fastq
Input file:	SRR25558502_1.fastq
Paired file:	SRR25558502_2.fastq
trimmed:	SRR25558502-trimmed-pair1.fastq, SRR25558502-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 16:00:27 2026 >> started

Sun May 24 16:00:27 2026 >> done (0.025s)
9212 read pairs processed; of these:
  14 ( 0.15%) short read pairs filtered out after trimming by size control
  10 ( 0.11%) empty read pairs filtered out after trimming by size control
9188 (99.74%) read pairs available; of these:
1159 (12.61%) trimmed read pairs available after processing
8029 (87.39%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 60	   1	  0.01%
 61	   3	  0.03%
 62	   1	  0.01%
 63	   0	  0.00%
 64	   2	  0.02%
 65	   1	  0.01%
 66	   1	  0.01%
 67	   0	  0.00%
 68	   2	  0.02%
 69	   0	  0.00%
 70	   0	  0.00%
 71	   1	  0.01%
 72	   0	  0.00%
 73	   0	  0.00%
 74	   3	  0.03%
 75	   2	  0.02%
 76	   1	  0.01%
 77	   1	  0.01%
 78	   2	  0.02%
 79	   0	  0.00%
 80	   0	  0.00%
 81	   9	  0.10%
 82	  14	  0.15%
 83	  15	  0.16%
 84	  22	  0.24%
 85	  30	  0.33%
 86	  24	  0.26%
 87	  31	  0.34%
 88	  25	  0.27%
 89	  36	  0.39%
 90	  38	  0.41%
 91	  30	  0.33%
 92	  36	  0.39%
 93	  45	  0.49%
 94	  50	  0.54%
 95	  57	  0.62%
 96	  59	  0.64%
 97	  78	  0.85%
 98	  71	  0.77%
 99	 113	  1.23%
100	 355	  3.86%
101	8029	 87.39%
9188 reads passed initial QC


criterion=sequence-density
sequence-density=8.29
sequence-density-rank=1
fanout-score=8.39
fanout-score-rank=1
prefix-density=22.61
prefix-fanout=3.1
sequence=CTTTTTCTTGAG


criterion=fanout-score
sequence-density=8.29
sequence-density-rank=1
fanout-score=8.39
fanout-score-rank=1
prefix-density=22.61
prefix-fanout=3.1
sequence=CTTTTTCTTGAG


criterion=sequence-density
sequence-density=16.91
sequence-density-rank=1
fanout-score=7.12
fanout-score-rank=1
prefix-density=37.08
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=16.91
sequence-density-rank=1
fanout-score=7.12
fanout-score-rank=1
prefix-density=37.08
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x CTTTTTCTTGAG -y AAGAAAAAGGTTCC -o SRR25558502 SRR25558502_1.fastq SRR25558502_2.fastq
Input file:	SRR25558502_1.fastq
Paired file:	SRR25558502_2.fastq
trimmed:	SRR25558502-trimmed-pair1.fastq, SRR25558502-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTTTTTCTTGAG
-- paired 3' end adapter sequence (-y):	AAGAAAAAGGTTCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 16:00:29 2026 >> started

Sun May 24 16:00:29 2026 >> done (0.015s)
7775 read pairs processed; of these:
   3 ( 0.04%) short read pairs filtered out after trimming by size control
  13 ( 0.17%) empty read pairs filtered out after trimming by size control
7759 (99.79%) read pairs available; of these:
   7 ( 0.09%) trimmed read pairs available after processing
7752 (99.91%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 60	   1	  0.01%
 61	   2	  0.03%
 62	   1	  0.01%
 63	   0	  0.00%
 64	   2	  0.03%
 65	   1	  0.01%
 66	   1	  0.01%
 67	   0	  0.00%
 68	   2	  0.03%
 69	   0	  0.00%
 70	   0	  0.00%
 71	   1	  0.01%
 72	   0	  0.00%
 73	   0	  0.00%
 74	   3	  0.04%
 75	   2	  0.03%
 76	   1	  0.01%
 77	   1	  0.01%
 78	   2	  0.03%
 79	   0	  0.00%
 80	   0	  0.00%
 81	   7	  0.09%
 82	  13	  0.17%
 83	  13	  0.17%
 84	  20	  0.26%
 85	  26	  0.34%
 86	  20	  0.26%
 87	  28	  0.36%
 88	  21	  0.27%
 89	  33	  0.43%
 90	  35	  0.45%
 91	  23	  0.30%
 92	  32	  0.41%
 93	  41	  0.53%
 94	  40	  0.52%
 95	  50	  0.64%
 96	  51	  0.66%
 97	  59	  0.76%
 98	  60	  0.77%
 99	  91	  1.17%
100	 306	  3.94%
101	6770	 87.25%


criterion=sequence-density
sequence-density=8.08
sequence-density-rank=1
fanout-score=8.47
fanout-score-rank=1
prefix-density=22.33
prefix-fanout=3.1
sequence=CTTTTTCTTGAG


criterion=fanout-score
sequence-density=8.08
sequence-density-rank=1
fanout-score=8.47
fanout-score-rank=1
prefix-density=22.33
prefix-fanout=3.1
sequence=CTTTTTCTTGAG


criterion=sequence-density
sequence-density=16.70
sequence-density-rank=1
fanout-score=7.13
fanout-score-rank=1
prefix-density=36.75
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=16.70
sequence-density-rank=1
fanout-score=7.13
fanout-score-rank=1
prefix-density=36.75
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC
SRR25558502 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 16:00:54
                             Started mapping on |	May 24 16:00:54
                                    Finished on |	May 24 16:00:57
       Mapping speed, Million of reads per hour |	11.01

                          Number of input reads |	9172
                      Average input read length |	200
                                    UNIQUE READS:
                   Uniquely mapped reads number |	8242
                        Uniquely mapped reads % |	89.86%
                          Average mapped length |	199.77
                       Number of splices: Total |	10375
            Number of splices: Annotated (sjdb) |	10365
                       Number of splices: GT/AG |	10372
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	3
                      Mismatch rate per base, % |	0.10%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.28
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	669
             % of reads mapped to multiple loci |	7.29%
        Number of reads mapped to too many loci |	7
             % of reads mapped to too many loci |	0.08%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.77%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	271	271	271
N_multimapping	669	669	669
N_noFeature	20	8238	22
N_ambiguous	52	0	50
UnstrandedReadsAssigned:8170 PositiveStrandReadsAssigned:4 NegativeStrandReadsAssigned:8170
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR25558502 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558502-trimmed-pair1.fastq
                             SRR25558502-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 9,172 reads, 8,887 reads pseudoaligned
[quant] estimated average fragment length: 138.888
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

 109283 SRR25558502.ke.tsv
  52636 SRR25558502.se.tsv
 161919 total
==> SRR25558502.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25558502.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558502 completed mapping pipeline successfully
