Starting /dee2/code/volunteer_pipeline.sh SRR25558503
    current disk space = 460523054080
    free memory = 343508684 
SRR25558503 SRAfilesize
966340782f46a937c5dcfdcc29ab40fd  SRR25558503.sra
SRR25558503.sra file validated
SRR25558503 is paired end
SRR25558503 is conventional basespace
SRR25558503 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558503_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3991
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94788273615635	34.0	33.0	34.0	32.0	34.0
2	33.02079679278376	34.0	33.0	34.0	32.0	34.0
3	33.06664996241543	34.0	33.0	34.0	32.0	34.0
4	33.050112753695814	34.0	33.0	34.0	32.0	34.0
5	32.98872463041844	34.0	33.0	34.0	32.0	34.0
6	36.71235279378602	38.0	37.0	38.0	34.0	38.0
7	37.08694562766224	38.0	38.0	38.0	36.0	38.0
8	37.14482585818091	38.0	38.0	38.0	36.0	38.0
9	37.19819594086695	38.0	38.0	38.0	36.0	38.0
10-11	37.2072162365322	38.0	38.0	38.0	36.0	38.0
12-13	37.14908544224505	38.0	38.0	38.0	36.0	38.0
14-15	37.17439238286144	38.0	38.0	38.0	36.0	38.0
16-17	37.21661237785016	38.0	38.0	38.0	36.5	38.0
18-19	37.2382861438236	38.0	38.0	38.0	37.0	38.0
20-21	37.17376597344024	38.0	38.0	38.0	37.0	38.0
22-23	37.14445001252819	38.0	38.0	38.0	36.0	38.0
24-25	37.09546479579053	38.0	38.0	38.0	36.0	38.0
26-27	37.08669506389376	38.0	38.0	38.0	36.0	38.0
28-29	37.06113755950889	38.0	38.0	38.0	36.0	38.0
30-31	37.117890253069405	38.0	38.0	38.0	36.0	38.0
32-33	37.04522676021047	38.0	38.0	38.0	36.0	38.0
34-35	37.118516662490606	38.0	38.0	38.0	36.5	38.0
36-37	37.09546479579053	38.0	38.0	38.0	36.0	38.0
38-39	36.90679027812578	38.0	38.0	38.0	36.0	38.0
40-41	37.03933851165121	38.0	38.0	38.0	36.0	38.0
42-43	37.000876973189676	38.0	38.0	38.0	36.0	38.0
44-45	36.97569531445753	38.0	38.0	38.0	36.0	38.0
46-47	36.986218992733654	38.0	38.0	38.0	36.0	38.0
48-49	36.94537709847156	38.0	38.0	38.0	36.0	38.0
50-51	36.848033074417444	38.0	38.0	38.0	35.0	38.0
52-53	36.89413680781759	38.0	38.0	38.0	35.5	38.0
54-55	36.93585567526935	38.0	38.0	38.0	36.0	38.0
56-57	36.913179654222	38.0	38.0	38.0	35.5	38.0
58-59	36.980330744174395	38.0	38.0	38.0	36.0	38.0
60-61	36.9724379854673	38.0	38.0	38.0	35.5	38.0
62-63	36.8586820345778	38.0	38.0	38.0	35.0	38.0
64-65	36.92746178902531	38.0	38.0	38.0	35.5	38.0
66-67	36.90440992232523	38.0	38.0	38.0	35.5	38.0
68-69	36.89526434477575	38.0	38.0	38.0	35.5	38.0
70-71	36.9024054121774	38.0	38.0	38.0	35.5	38.0
72-73	36.914432473064394	38.0	38.0	38.0	35.5	38.0
74-75	36.83763467802556	38.0	38.0	38.0	35.0	38.0
76-77	36.84815835630168	38.0	38.0	38.0	35.0	38.0
78-79	36.771109997494364	38.0	38.0	38.0	35.0	38.0
80-81	36.77148584314708	38.0	38.0	38.0	35.0	38.0
82-83	36.80543723377599	38.0	38.0	38.0	35.0	38.0
84-85	36.6143823603107	38.0	38.0	38.0	34.5	38.0
86-87	36.75068905036332	38.0	38.0	38.0	35.0	38.0
88-89	36.75958406414432	38.0	38.0	38.0	35.0	38.0
90-91	36.808068153345026	38.0	38.0	38.0	35.0	38.0
92-93	36.71986970684039	38.0	38.0	38.0	35.0	38.0
94-95	36.51791530944625	38.0	38.0	38.0	34.0	38.0
96-97	36.457028313705834	38.0	38.0	38.0	34.0	38.0
98-99	36.34778251064896	38.0	38.0	38.0	34.0	38.0
100-101	35.69844650463543	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	0.0
22	2.0
23	0.0
24	4.0
25	9.0
26	11.0
27	20.0
28	16.0
29	32.0
30	54.0
31	55.0
32	65.0
33	81.0
34	126.0
35	194.0
36	405.0
37	2914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.96084337349398	8.057228915662652	10.441767068273093	47.540160642570285
2	21.373089451265347	9.120521172638437	38.38636933099474	31.120020045101477
3	18.541718867451767	11.726384364820847	27.56201453269857	42.169882235028815
4	27.110999749436232	19.719368579303435	25.482335254322226	27.68729641693811
5	19.09295915810574	31.946880481082434	28.18842395389627	20.77173640691556
6	15.935855675269355	30.64394888499123	33.65071410674017	19.76948133299925
7	17.38912553244801	17.46429466299173	48.50914557754948	16.637434227010775
8	18.74216988223503	26.384364820846905	34.101728890002505	20.77173640691556
9	14.607867702330243	23.402655975945876	38.03558005512403	23.95389626659985
10-11	21.373089451265347	28.36381859183162	28.71460786770233	21.548484089200702
12-13	23.86619894763217	19.99498872463042	31.784014031571033	24.354798296166376
14-15	20.12027060886996	22.60085191681283	36.13129541468304	21.147582059634175
16-17	21.122525682786268	21.824104234527688	31.87171135053871	25.181658732147334
18-19	20.80932097218742	21.67376597344024	28.915058882485596	28.601854171886743
20-21	20.45853169631671	22.70107742420446	30.76923076923077	26.07116011024806
22-23	28.263593084439993	24.906038586820344	26.259082936607363	20.571285392132296
24-25	23.816086193936357	25.131545978451513	27.762465547481835	23.289902280130292
26-27	23.139564019042847	24.71811576046104	28.22600851916813	23.916311701327988
28-29	20.784264595339515	28.05061388123277	21.022300175394637	30.14282134803307
30-31	23.101979453770983	24.617890253069405	24.730643948884993	27.549486344274616
32-33	21.037463976945244	25.159754416739755	27.903771457210873	25.899010149104125
34-35	20.99724379854673	23.11450764219494	29.5665246805312	26.321723878727138
36-37	21.81704260651629	24.51127819548872	26.228070175438596	27.44360902255639
38-39	23.075958886939084	27.036851341188267	28.45324642767611	21.43394334419654
40-41	23.727751316119328	23.815492604662822	28.365505139132612	24.091250940085235
42-43	23.609022556390975	21.17794486215539	29.022556390977446	26.190476190476193
44-45	18.58861870142893	27.88919528703936	30.15793431937829	23.364251692153424
46-47	21.772374028578593	22.75006267234896	28.566056655803457	26.91150664326899
48-49	22.57458009526197	25.457508147405367	23.289044873401853	28.67886688393081
50-51	25.576441102756892	20.538847117794486	31.654135338345863	22.230576441102755
52-53	20.145326985717865	23.703332498120773	30.83187171135054	25.319468804810825
54-55	18.49160611375595	21.811576046103735	32.7987972939113	26.898020546229017
56-57	23.753445251816586	22.41292909045352	32.047105988474065	21.786519669255824
58-59	19.35605111500877	25.84565271861689	31.15760461037334	23.640691556001002
60-61	22.12478075670258	28.82736156351792	27.298922575795544	21.748935103983964
62-63	19.83212227511902	22.362816336757703	35.11651215234277	22.688549235780506
64-65	19.468804810824356	22.80130293159609	31.044850914557752	26.685041343021798
66-67	19.468804810824356	22.225006264094212	32.15985968428966	26.146329240791783
68-69	20.170383362565772	24.668003006765222	27.499373590578802	27.662240040090204
70-71	19.594086695063893	22.876472062139815	30.719118015534953	26.81032322726134
72-73	20.0826860435981	24.11676271611125	27.323978952643447	28.476572287647205
74-75	21.96191430719118	24.11676271611125	27.349035329491358	26.572287647206217
76-77	24.99373590578802	21.598596842896516	30.543723377599598	22.86394387371586
78-79	22.300175394637936	22.575795539964922	32.12227511901779	23.001753946379353
80-81	22.613380105236782	22.70107742420446	32.1473314958657	22.538210974693058
82-83	20.60886995740416	21.799047857679778	30.51866700075169	27.073415184164368
84-85	21.42320220496116	25.795539964921073	28.30117764971185	24.480080180405913
86-87	22.939113004259585	24.50513655725382	29.278376346780256	23.277374091706342
88-89	18.291155098972688	30.756702580806817	23.978952643447755	26.97318967677274
90-91	22.18742169882235	28.55174141819093	22.074668003006764	27.186168879979956
92-93	23.227261338010525	27.762465547481835	23.390127787521926	25.620145326985718
94-95	27.875219243297416	25.131545978451513	25.833124530192936	21.160110248058132
96-97	29.754447506890504	24.592833876221498	21.44825858180907	24.204460035078927
98-99	24.71811576046104	20.646454522676024	24.943623152092208	29.691806564770733
100-101	19.21726041144004	27.09483191169092	25.376317109884596	28.311590566984446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	1.0
31	1.0
32	0.0
33	1.0
34	2.5
35	2.5
36	7.5
37	8.0
38	4.5
39	16.0
40	52.0
41	87.0
42	63.5
43	63.0
44	162.0
45	332.5
46	396.5
47	542.5
48	626.5
49	411.0
50	287.5
51	375.5
52	370.0
53	146.5
54	17.0
55	7.0
56	5.0
57	2.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17539463793535454
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.012528188423953897
34-35	0.0
36-37	0.025056376847907794
38-39	0.05011275369581559
40-41	0.05011275369581559
42-43	0.025056376847907794
44-45	0.05011275369581559
46-47	0.05011275369581559
48-49	0.05011275369581559
50-51	0.025056376847907794
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.12528188423953898
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.812828864946127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.80029806259314	9.045352042094713
2	11.177347242921014	3.7584565271861687
3	7.153502235469449	3.608118266098722
4	2.235469448584203	1.5033826108744675
5	2.682563338301043	2.2550739163117015
6	2.9806259314456036	3.006765221748935
7	2.235469448584203	2.6309195690303184
8	1.1922503725782414	1.6036081182660988
9	0.8941877794336811	1.3530443497870208
>10	14.157973174366617	52.367827612127286
>50	1.1922503725782414	12.95414683036833
>100	0.29806259314456035	5.913304936106239
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCA	132	3.3074417439238286	No Hit
CTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTTCTGCG	104	2.6058631921824107	No Hit
TGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGGG	85	2.1297920320721624	No Hit
CTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCAT	84	2.1047356552242547	No Hit
CTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGG	68	1.7038336256577298	No Hit
GTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAAC	63	1.5785517414181909	No Hit
GCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGT	59	1.4783262340265597	No Hit
GCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAG	56	1.4031571034828363	No Hit
GTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCT	51	1.2778752192432974	No Hit
GGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGAT	51	1.2778752192432974	No Hit
CCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGT	49	1.2277624655474817	No Hit
CAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCAT	47	1.1776497118516662	No Hit
GCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTT	46	1.1525933350037585	No Hit
GCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATC	45	1.1275369581558508	No Hit
GCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAA	45	1.1275369581558508	No Hit
GTCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAG	44	1.1024805813079428	No Hit
CCTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAA	41	1.0273114507642196	No Hit
CTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAAT	40	1.0022550739163119	No Hit
GCCAGCTTTCCTTGCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCT	40	1.0022550739163119	No Hit
ATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTG	37	0.9270859433725883	No Hit
CCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTA	37	0.9270859433725883	No Hit
GCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACT	36	0.9020295665246805	No Hit
CTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGC	36	0.9020295665246805	No Hit
CTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCT	36	0.9020295665246805	No Hit
GTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAG	35	0.8769731896767727	No Hit
CTCGAATTCTGATGACAAAGGCCAGCTTTGGTTCTGCGGGCACATAGAAG	33	0.8268604359809572	No Hit
CTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAA	32	0.8018040591330494	No Hit
GGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGG	31	0.7767476822851416	No Hit
CCTTGCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACT	31	0.7767476822851416	No Hit
GTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGGGCTTACTCCA	30	0.7516913054372337	No Hit
AGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	30	0.7516913054372337	No Hit
GTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCA	30	0.7516913054372337	No Hit
TGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGA	30	0.7516913054372337	No Hit
GGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCG	29	0.726634928589326	No Hit
CGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTAC	28	0.7015785517414181	No Hit
GTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTC	27	0.6765221748935104	No Hit
CCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTG	25	0.6264094211976947	No Hit
CCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCC	24	0.601353044349787	No Hit
CCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCA	24	0.601353044349787	No Hit
CCTGGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCCTTGTGAT	23	0.5762966675018792	No Hit
CTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTG	23	0.5762966675018792	No Hit
CGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAAC	23	0.5762966675018792	No Hit
CGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACC	22	0.5512402906539714	No Hit
GCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCC	22	0.5512402906539714	No Hit
GCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAG	22	0.5512402906539714	No Hit
CACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTG	22	0.5512402906539714	No Hit
CTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCA	22	0.5512402906539714	No Hit
GCCAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGG	22	0.5512402906539714	No Hit
CCCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAA	22	0.5512402906539714	No Hit
CGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAA	21	0.5261839138060637	No Hit
GTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCT	21	0.5261839138060637	No Hit
CATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTTCTGCGGGC	21	0.5261839138060637	No Hit
CCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGA	21	0.5261839138060637	No Hit
CTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGA	21	0.5261839138060637	No Hit
CCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCT	20	0.5011275369581559	No Hit
GGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGT	20	0.5011275369581559	No Hit
CTTTGGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCT	20	0.5011275369581559	No Hit
CACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTC	19	0.476071160110248	No Hit
ATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGT	19	0.476071160110248	No Hit
CTTGCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTC	19	0.476071160110248	No Hit
CTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCA	18	0.45101478326234024	No Hit
CGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTT	18	0.45101478326234024	No Hit
CCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTA	18	0.45101478326234024	No Hit
CCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCAC	18	0.45101478326234024	No Hit
ATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTG	18	0.45101478326234024	No Hit
ATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATT	18	0.45101478326234024	No Hit
CGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGC	18	0.45101478326234024	No Hit
CCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCT	18	0.45101478326234024	No Hit
GTCAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGAT	18	0.45101478326234024	No Hit
CGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACA	17	0.42595840641443244	No Hit
GCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCCTT	17	0.42595840641443244	No Hit
TGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGT	17	0.42595840641443244	No Hit
CGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGGGCTTACTCCATT	16	0.4009020295665247	No Hit
ATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTTCTGCGGGCA	15	0.37584565271861686	No Hit
GGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAA	15	0.37584565271861686	No Hit
ACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCG	15	0.37584565271861686	No Hit
GCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCT	15	0.37584565271861686	No Hit
TTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGA	15	0.37584565271861686	No Hit
CATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTC	14	0.35078927587070907	No Hit
GCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAG	14	0.35078927587070907	No Hit
TCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTTCTGCGG	14	0.35078927587070907	No Hit
TAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCT	14	0.35078927587070907	No Hit
CTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACT	14	0.35078927587070907	No Hit
GGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCCTTGTGATAGT	13	0.32573289902280134	No Hit
CCAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGT	13	0.32573289902280134	No Hit
CCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTT	13	0.32573289902280134	No Hit
GTTGTCTTTTTCTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCA	13	0.32573289902280134	No Hit
CAAGCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTG	13	0.32573289902280134	No Hit
TGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTT	13	0.32573289902280134	No Hit
CGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTAC	12	0.3006765221748935	No Hit
CCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTTCTGCGGG	12	0.3006765221748935	No Hit
ACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCA	12	0.3006765221748935	No Hit
GTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCT	12	0.3006765221748935	No Hit
CTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGT	11	0.2756201453269857	No Hit
CATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTT	11	0.2756201453269857	No Hit
CCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTT	11	0.2756201453269857	No Hit
CCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTG	11	0.2756201453269857	No Hit
CCGACTGTATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAA	11	0.2756201453269857	No Hit
GGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGT	11	0.2756201453269857	No Hit
TGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACC	11	0.2756201453269857	No Hit
ATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCC	10	0.25056376847907796	No Hit
GTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAA	10	0.25056376847907796	No Hit
GTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTC	10	0.25056376847907796	No Hit
GCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCAC	10	0.25056376847907796	No Hit
CTAATAAGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTC	10	0.25056376847907796	No Hit
CTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTG	9	0.22550739163117012	No Hit
GCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAG	9	0.22550739163117012	No Hit
GAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGTTGATTTTG	9	0.22550739163117012	No Hit
CATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTT	9	0.22550739163117012	No Hit
GGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTA	9	0.22550739163117012	No Hit
GCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCA	9	0.22550739163117012	No Hit
CGGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTG	8	0.20045101478326235	No Hit
CTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTC	8	0.20045101478326235	No Hit
GTTGAGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCT	8	0.20045101478326235	No Hit
TTCAGGTTGGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCAT	8	0.20045101478326235	No Hit
CAAGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGA	8	0.20045101478326235	No Hit
GCCAGCTTTGGTTCTGCGGGCACATAGAAGTTGCCAGCTTTCCTTGCCAT	8	0.20045101478326235	No Hit
GCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGA	8	0.20045101478326235	No Hit
GAACCTTTGGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCC	8	0.20045101478326235	No Hit
TCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTT	7	0.17539463793535454	No Hit
CGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACC	7	0.17539463793535454	No Hit
GGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTT	7	0.17539463793535454	No Hit
TGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAA	7	0.17539463793535454	No Hit
AGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATG	7	0.17539463793535454	No Hit
CATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTAT	7	0.17539463793535454	No Hit
CCCCTCTCAAAAAACACAAAACAAAAAACACTTAACAATATACCCAATCC	7	0.17539463793535454	No Hit
CCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTT	7	0.17539463793535454	No Hit
TATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATC	7	0.17539463793535454	No Hit
CTGGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCCTTGTGATA	7	0.17539463793535454	No Hit
CTGCAGAACCTTACGAACCTTTGGGCTTACTCCATTGATACCTCGAATTC	7	0.17539463793535454	No Hit
GGCACATAGAAGTTGCCAGCTTTCCTTGCCATCCTGGCCATCCGAATCTC	7	0.17539463793535454	No Hit
GGGGTACCCCCATGCAATGTATGGCTCCACAATCCGCAGCATGTTAATTG	7	0.17539463793535454	No Hit
TGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCCCTG	7	0.17539463793535454	No Hit
GGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGA	7	0.17539463793535454	No Hit
GTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACC	6	0.15033826108744675	No Hit
CGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAA	6	0.15033826108744675	No Hit
CAAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTG	6	0.15033826108744675	No Hit
CGGGCACATAGAAGTTGCCAGCTTTCCTTGCCATCCTGGCCATCCGAATC	6	0.15033826108744675	No Hit
TCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGT	6	0.15033826108744675	No Hit
CTCATGAATTAGATCCTCCATGCAGATGATGCCAAACTTACCAAGAGACC	6	0.15033826108744675	No Hit
GAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGGGC	6	0.15033826108744675	No Hit
GTCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAAGTGAGTTGT	6	0.15033826108744675	No Hit
ACCCAATCCGCACTCAACAGAAAAGCCGTCAATCAAGTTTTGAATTCAAA	6	0.15033826108744675	No Hit
GCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCCTTGTGATAGTG	6	0.15033826108744675	No Hit
TCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGGGCTTACTCCAT	6	0.15033826108744675	No Hit
GAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCT	6	0.15033826108744675	No Hit
TATAGATCTCATGAATTAGATCCTCCATGCAGATGATGCCAAACTTACCA	6	0.15033826108744675	No Hit
TGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTT	6	0.15033826108744675	No Hit
CTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTGGTTC	6	0.15033826108744675	No Hit
ATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATC	6	0.15033826108744675	No Hit
GCTGTGACTGTTTATTAACTGACCAGATTACAAAAATACAATGGGTGACA	6	0.15033826108744675	No Hit
GGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAGA	6	0.15033826108744675	No Hit
CTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGGGCTTACTC	6	0.15033826108744675	No Hit
CCAGCTTTCCTTGCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCTG	6	0.15033826108744675	No Hit
GTTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTG	5	0.12528188423953898	No Hit
TTGCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCC	5	0.12528188423953898	No Hit
AGGCAATCCGCTTCTTGTTGATTTTGCCGTAGCCTCGCTTGTAGATGAGC	5	0.12528188423953898	No Hit
CTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTATTTGCTTCCTTG	5	0.12528188423953898	No Hit
TTGAAGATCTGTCGAAGACGAAGGAGCTGCAGAACCTTACGAACCTTTGG	5	0.12528188423953898	No Hit
TGGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTG	5	0.12528188423953898	No Hit
CGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	5	0.12528188423953898	No Hit
AGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGAT	5	0.12528188423953898	No Hit
GTCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATT	5	0.12528188423953898	No Hit
GCTGCAGAACCTTACGAACCTTTGGGCTTACTCCATTGATACCTCGAATT	5	0.12528188423953898	No Hit
GGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATGTATGGCTC	5	0.12528188423953898	No Hit
GGGTGACACCTTAGTTCATCCGTCTAATAAGCCTGTTTATCTGGTCTTCC	5	0.12528188423953898	No Hit
AGCTGCAGAACCTTACGAACCTTTGGGCTTACTCCATTGATACCTCGAAT	5	0.12528188423953898	No Hit
GCTTTCCTTGCCATCCTGGCCATCCGAATCTCAGTGCGGTACATCTGCCT	5	0.12528188423953898	No Hit
AAGAGACCGAGCAATCAAGGAATTATCTGTCAAGGCAATCCGCTTCTTGT	5	0.12528188423953898	No Hit
CTTCATCCCACCTCGTGGGGAAGATAACTTGAAGGGCCACAGGAAGTTAT	5	0.12528188423953898	No Hit
TAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCATCCGT	5	0.12528188423953898	No Hit
GCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGGAATTATCTG	5	0.12528188423953898	No Hit
GCAGAACCTTACGAACCTTTGGGCTTACTCCATTGATACCTCGAATTCTG	4	0.10022550739163118	No Hit
AGGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTG	4	0.10022550739163118	No Hit
TTATTAACTGACCAGATTACAAAAATACAATGGGTGACACCTTAGTTCAT	4	0.10022550739163118	No Hit
GCTTCCTTGAAGCGTTTCCCGACTGTATAGATCTCATGAATTAGATCCTC	4	0.10022550739163118	No Hit
GCATGTTAATTGAAGCCTTGTTGAGCTTAACAAAGGTGCCATTGAAGATC	4	0.10022550739163118	No Hit
CGGGCCACAGGAAGTTATTTGCTTCCTTGAAGCGTTTCCCGACTGTATAG	4	0.10022550739163118	No Hit
ATCCTCCATGCAGATGATGCCAAACTTACCAAGAGACCGAGCAATCAAGG	4	0.10022550739163118	No Hit
GGGGCTTACTCCATTGATACCTCGAATTCTGATGACAAAGGCCAGCTTTG	4	0.10022550739163118	No Hit
TGTAGATGAGCTCGTTTACTGACTTCAGGTTGGGGTACCCCCATGCAATG	4	0.10022550739163118	No Hit
CCAGCATCTCCACCTTCTACAAAGTGAGTTGTCTTTTTCTTCATCCCACC	4	0.10022550739163118	No Hit
AGCTTAACAAAGGTGCCATTGAAGATCTGTCGAAGACGAAGGAGCTGCAG	4	0.10022550739163118	No Hit
ATCCGAATCTCAGTGCGGTACATCTGCCTGTACTCCTTGTGATAGTGCTT	4	0.10022550739163118	No Hit
CCTTACGAACCTTTGGGCTTACTCCATTGATACCTCGAATTCTGATGACA	4	0.10022550739163118	No Hit
ATTTTGCCGTAGCCTCGCTTGTAGATGAGCTCGTTTACTGACTTCAGGTT	4	0.10022550739163118	No Hit
GGCCTGTTTATCTGGTCTTCCCTGTTGCCAGCATCTCCACCTTCTACAAA	4	0.10022550739163118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.012528188423953895	0.0	0.0	0.0	0.0
82-83	0.20045101478326233	0.0	0.0	0.0	0.0
84-85	0.826860435980957	0.0	0.0	0.0	0.0
86-87	1.941869205712854	0.0	0.0	0.0	0.0
88-89	2.8313705838135803	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25558503 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558503_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3986
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.605368790767685	33.0	33.0	34.0	32.0	34.0
2	32.735323632714504	33.0	33.0	34.0	32.0	34.0
3	32.81083793276468	34.0	33.0	34.0	32.0	34.0
4	32.75639739086804	34.0	33.0	34.0	32.0	34.0
5	32.8509784244857	34.0	33.0	34.0	32.0	34.0
6	36.846462619167085	38.0	38.0	38.0	35.0	38.0
7	36.87305569493226	38.0	38.0	38.0	36.0	38.0
8	36.894631209232315	38.0	38.0	38.0	36.0	38.0
9	36.88911189162067	38.0	38.0	38.0	36.0	38.0
10-11	36.88095835423984	38.0	38.0	38.0	36.0	38.0
12-13	36.88798294029102	38.0	38.0	38.0	36.0	38.0
14-15	36.83291520321124	38.0	38.0	38.0	35.5	38.0
16-17	36.79967385850477	38.0	38.0	38.0	35.5	38.0
18-19	36.874560963371806	38.0	38.0	38.0	35.5	38.0
20-21	36.78687907676869	38.0	38.0	38.0	35.0	38.0
22-23	36.81999498243854	38.0	38.0	38.0	35.5	38.0
24-25	36.84157049673858	38.0	38.0	38.0	35.0	38.0
26-27	36.782865027596586	38.0	38.0	38.0	35.0	38.0
28-29	36.74222277972905	38.0	38.0	38.0	35.0	38.0
30-31	36.70697441043653	38.0	38.0	38.0	35.0	38.0
32-33	36.687405920722526	38.0	38.0	38.0	35.0	38.0
34-35	36.720647265429	38.0	38.0	38.0	35.0	38.0
36-37	36.716633216256895	38.0	38.0	38.0	35.0	38.0
38-39	36.803186151530355	38.0	38.0	38.0	35.5	38.0
40-41	36.74259909683894	38.0	38.0	38.0	35.0	38.0
42-43	36.71801304565981	38.0	38.0	38.0	35.0	38.0
44-45	36.7606623181134	38.0	38.0	38.0	35.0	38.0
46-47	36.75225790265931	38.0	38.0	38.0	35.0	38.0
48-49	36.73030607124937	38.0	38.0	38.0	35.0	38.0
50-51	36.70923231309584	38.0	38.0	38.0	35.0	38.0
52-53	36.67511289513297	38.0	38.0	38.0	35.0	38.0
54-55	36.63635223281485	38.0	38.0	38.0	34.5	38.0
56-57	36.67197691921726	38.0	38.0	38.0	35.0	38.0
58-59	36.710612142498746	38.0	38.0	38.0	35.0	38.0
60-61	36.676367285499246	38.0	38.0	38.0	34.5	38.0
62-63	36.65403913697943	38.0	38.0	38.0	34.5	38.0
64-65	36.54076768690417	38.0	38.0	38.0	34.5	38.0
66-67	36.62481184144506	38.0	38.0	38.0	34.5	38.0
68-69	36.61552935273457	38.0	38.0	38.0	35.0	38.0
70-71	36.56573005519317	38.0	38.0	38.0	34.0	38.0
72-73	36.65654791771199	38.0	38.0	38.0	35.0	38.0
74-75	36.574259909683896	38.0	38.0	38.0	34.0	38.0
76-77	36.515303562468645	38.0	38.0	38.0	34.0	38.0
78-79	36.51743602609132	38.0	38.0	38.0	34.0	38.0
80-81	36.426618163572506	38.0	38.0	38.0	34.0	38.0
82-83	36.47967887606623	38.0	38.0	38.0	34.0	38.0
84-85	36.51580531861515	38.0	38.0	38.0	34.0	38.0
86-87	36.43840943301555	38.0	38.0	38.0	34.0	38.0
88-89	36.42285499247366	38.0	38.0	38.0	34.0	38.0
90-91	36.27333166081284	38.0	38.0	38.0	34.0	38.0
92-93	36.27860010035123	38.0	38.0	38.0	34.0	38.0
94-95	36.19831911690918	38.0	38.0	38.0	33.5	38.0
96-97	36.19819367787255	38.0	38.0	38.0	34.0	38.0
98-99	36.11088810837933	38.0	38.0	38.0	34.0	38.0
100-101	34.97955343702961	38.0	36.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	1.0
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	2.0
19	0.0
20	3.0
21	8.0
22	12.0
23	8.0
24	18.0
25	17.0
26	18.0
27	23.0
28	41.0
29	44.0
30	38.0
31	56.0
32	68.0
33	87.0
34	109.0
35	182.0
36	386.0
37	2857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.553437029603614	18.615153035624687	8.580030105368792	43.25137982940291
2	25.840441545408932	25.965880582037133	30.8078273958856	17.385850476668338
3	18.138484696437533	28.324134470647266	30.8078273958856	22.729553437029605
4	21.600602107375817	37.907676869041644	17.335674862017058	23.15604616156548
5	29.402910185649773	39.21224284997491	15.4039136979428	15.980933266432515
6	21.525338685398896	42.12242849974912	17.862518815855495	18.48971399899649
7	25.263421976919215	18.740592072252884	34.64626191670848	21.34972403411942
8	23.883592574009032	24.28499749121927	27.99799297541395	23.833416959357752
9	23.733065730055195	26.367285499247366	25.865529352734573	24.03411941796287
10-11	25.426492724535876	34.909683893627694	18.151028600100354	21.512794781736076
12-13	23.77069744104365	26.27947817360763	25.188158554942298	24.761665830406425
14-15	24.234821876567988	28.988961364776717	25.23833416959358	21.537882589061716
16-17	25.0	28.173607626693425	21.274460612142498	25.551931761164077
18-19	24.686402408429505	29.942298043151027	25.57701956848971	19.794279979929755
20-21	27.69693928750627	27.157551430005018	23.507275464124437	21.638233818364274
22-23	26.216758655293525	29.252383341695936	23.30657300551932	21.224284997491218
24-25	26.329653788258906	27.170095333667838	24.08429503261415	22.415955845459106
26-27	25.100351229302557	30.030105368790768	22.905168088309082	21.964375313597593
28-29	27.75059591017438	28.729143143896625	21.816585121063856	21.703675824865137
30-31	25.200702458605118	28.650275965880585	21.211741093828397	24.9372804816859
32-33	23.168590065228297	32.85248369292524	22.955343702960363	21.023582538886103
34-35	25.589563472152534	28.77571500250878	23.507275464124437	22.12744606121425
36-37	23.36929252383342	31.49774209734069	23.519819367787257	21.613146011038637
38-39	25.89061716006021	28.662819869543405	24.72403411941796	20.722528850978424
40-41	25.2508780732564	31.096337180130458	21.036126442548923	22.616658304064224
42-43	24.96236828901154	28.96387355745108	23.03060712493728	23.0431510286001
44-45	24.535875564475663	29.84194681384847	25.163070747616658	20.45910687405921
46-47	26.27947817360763	26.128951329653788	23.181133968891118	24.410436527847466
48-49	24.234821876567988	30.318615153035626	24.00903161063723	21.437531359759156
50-51	24.29754139488209	30.56949322629202	23.26894129453086	21.864024084295032
52-53	27.358253888610136	26.906673356748623	22.742097340692425	22.99297541394882
54-55	24.109382839939787	29.967385850476667	24.109382839939787	21.813848469643755
56-57	22.353236327145005	31.096337180130458	22.36578023080783	24.184646261916708
58-59	26.881585549422983	25.288509784244855	26.103863522328147	21.726041144004014
60-61	26.417461113898643	32.5137982940291	21.575514300050173	19.49322629202208
62-63	27.87255393878575	26.693426994480685	24.799297541394882	20.634721525338687
64-65	26.693426994480685	27.32062217762168	24.799297541394882	21.18665328650276
66-67	25.03763171098846	28.386853988961363	24.611138986452584	21.964375313597593
68-69	23.1058705469142	29.06422478675364	26.417461113898643	21.412443552433515
70-71	25.551931761164077	30.218263923733062	23.432012042147516	20.79779227295534
72-73	24.661314601103864	27.508780732563977	22.742097340692425	25.087807325639737
74-75	26.128951329653788	29.214751630707475	23.181133968891118	21.475163070747616
76-77	24.97491219267436	28.93878575012544	20.434019066733565	25.652282990466635
78-79	25.878073256397393	31.096337180130458	24.184646261916708	18.840943301555445
80-81	21.726041144004014	30.58203712995484	23.858504766683392	23.833416959357752
82-83	25.514300050175613	29.13948820873056	21.738585047666835	23.607626693426994
84-85	22.81736076266934	29.202207727044655	24.322629202207725	23.657802308078274
86-87	25.050200803212853	32.166164658634536	22.038152610441767	20.745481927710845
88-89	26.02860010035123	27.935273457099854	25.827897641746112	20.20822880080281
90-91	28.695106649937262	26.3237139272271	26.3237139272271	18.657465495608534
92-93	24.507466432425648	31.33391893587652	21.219726439954826	22.938888191743004
94-95	26.01330154348099	28.698707491529678	21.897352239929727	23.390638725059606
96-97	30.263488080301133	30.815558343789213	19.33500627352572	19.58594730238394
98-99	24.639317526031864	31.376238865888844	24.237862250658637	19.746581357420652
100-101	24.372489959839356	26.49347389558233	26.92018072289157	22.213855421686745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	0.5
29	0.0
30	0.0
31	0.0
32	1.0
33	2.5
34	4.0
35	6.5
36	9.0
37	8.0
38	8.0
39	9.0
40	19.5
41	26.0
42	55.5
43	155.5
44	239.5
45	321.0
46	453.0
47	555.5
48	487.5
49	409.5
50	382.0
51	281.0
52	198.0
53	172.0
54	118.5
55	42.0
56	15.0
57	3.0
58	1.5
59	1.0
60	0.5
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.012543903662819869
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.050175614651279475
88-89	0.0
90-91	0.025087807325639738
92-93	0.03763171098845961
94-95	0.03763171098845961
96-97	0.025087807325639738
98-99	0.012543903662819869
100-101	0.050175614651279475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.86954340190667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.676767676767675	8.479678876066233
2	14.14141414141414	5.6196688409433015
3	7.575757575757576	4.515805318615153
4	5.555555555555555	4.415454089312594
5	5.05050505050505	5.017561465127947
6	3.0303030303030303	3.612644254892122
7	2.398989898989899	3.3366783743100856
8	2.146464646464646	3.411941796287005
9	2.272727272727273	4.064224786753638
>10	14.772727272727273	51.80632212744606
>50	0.3787878787878788	5.720020070245861
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCT	84	2.107375815353738	No Hit
CTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGC	73	1.8314099347717008	No Hit
CTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACT	71	1.7812343201204215	No Hit
CTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTAC	48	1.2042147516307076	No Hit
CTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAG	41	1.0286001003512293	No Hit
CGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAG	41	1.0286001003512293	No Hit
CAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGC	41	1.0286001003512293	No Hit
CGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGA	38	0.9533366783743101	No Hit
CCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCA	36	0.9031610637230305	No Hit
CTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCAC	34	0.8529854490717512	No Hit
GGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTC	34	0.8529854490717512	No Hit
GTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGA	33	0.8278976417461115	No Hit
CCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAA	31	0.7777220270948318	No Hit
CTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGC	30	0.7526342197691922	No Hit
CCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCC	29	0.7275464124435524	No Hit
CCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAA	29	0.7275464124435524	No Hit
GGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAG	29	0.7275464124435524	No Hit
CAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACAT	29	0.7275464124435524	No Hit
CTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAAT	27	0.6773707977922729	No Hit
CAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGG	27	0.6773707977922729	No Hit
GCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTG	27	0.6773707977922729	No Hit
GAAAAAGACAACTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAG	26	0.6522829904666332	No Hit
GGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAAT	24	0.6021073758153538	No Hit
CCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCA	24	0.6021073758153538	No Hit
CCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGAT	23	0.577019568489714	No Hit
CAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCA	23	0.577019568489714	No Hit
GTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGAT	22	0.5519317611640743	No Hit
CAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAAT	22	0.5519317611640743	No Hit
AATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCG	21	0.5268439538384345	No Hit
GTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTT	21	0.5268439538384345	No Hit
CTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATC	21	0.5268439538384345	No Hit
CCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCT	20	0.5017561465127948	No Hit
CCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAA	20	0.5017561465127948	No Hit
ACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTC	20	0.5017561465127948	No Hit
CTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATC	20	0.5017561465127948	No Hit
GGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGC	20	0.5017561465127948	No Hit
CTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAG	20	0.5017561465127948	No Hit
CTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCA	19	0.47666833918715507	No Hit
GTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTTTGTAG	18	0.45158053186151526	No Hit
GGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATAC	18	0.45158053186151526	No Hit
GCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTCA	18	0.45158053186151526	No Hit
GGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTA	17	0.4264927245358756	No Hit
CCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAA	17	0.4264927245358756	No Hit
CCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATC	17	0.4264927245358756	No Hit
GCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCT	17	0.4264927245358756	No Hit
CAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTA	17	0.4264927245358756	No Hit
TATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTC	17	0.4264927245358756	No Hit
GCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGT	17	0.4264927245358756	No Hit
AGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGG	16	0.4014049172102358	No Hit
ATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTG	16	0.4014049172102358	No Hit
GTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACA	16	0.4014049172102358	No Hit
CTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGT	16	0.4014049172102358	No Hit
CCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAG	16	0.4014049172102358	No Hit
CTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTA	16	0.4014049172102358	No Hit
CAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTAC	16	0.4014049172102358	No Hit
AACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCC	16	0.4014049172102358	No Hit
CTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTA	15	0.3763171098845961	No Hit
CTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGA	15	0.3763171098845961	No Hit
ATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAAT	15	0.3763171098845961	No Hit
GTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTT	15	0.3763171098845961	No Hit
TTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCG	15	0.3763171098845961	No Hit
AGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATT	15	0.3763171098845961	No Hit
GTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCG	15	0.3763171098845961	No Hit
CACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGG	15	0.3763171098845961	No Hit
GGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAA	15	0.3763171098845961	No Hit
CGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAA	15	0.3763171098845961	No Hit
CAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAG	15	0.3763171098845961	No Hit
GTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGA	14	0.35122930255895635	No Hit
GGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCT	14	0.35122930255895635	No Hit
GCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAA	14	0.35122930255895635	No Hit
CAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGA	14	0.35122930255895635	No Hit
CGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTA	14	0.35122930255895635	No Hit
CTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTG	14	0.35122930255895635	No Hit
AGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATG	14	0.35122930255895635	No Hit
CAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTC	14	0.35122930255895635	No Hit
AGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAG	14	0.35122930255895635	No Hit
CCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAG	14	0.35122930255895635	No Hit
CAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGG	14	0.35122930255895635	No Hit
AAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTG	14	0.35122930255895635	No Hit
ATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGC	13	0.3261414952333166	No Hit
AGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCA	13	0.3261414952333166	No Hit
CAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTC	13	0.3261414952333166	No Hit
GGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGT	13	0.3261414952333166	No Hit
CTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTT	13	0.3261414952333166	No Hit
GGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAG	13	0.3261414952333166	No Hit
AAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTC	13	0.3261414952333166	No Hit
GGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACA	12	0.3010536879076769	No Hit
CTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGC	12	0.3010536879076769	No Hit
CATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCT	12	0.3010536879076769	No Hit
GGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTG	12	0.3010536879076769	No Hit
ATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTC	12	0.3010536879076769	No Hit
GTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCAC	12	0.3010536879076769	No Hit
AGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGT	12	0.3010536879076769	No Hit
TGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAG	12	0.3010536879076769	No Hit
CAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGC	12	0.3010536879076769	No Hit
TTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTT	12	0.3010536879076769	No Hit
CTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCA	11	0.27596588058203714	No Hit
CAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCAT	11	0.27596588058203714	No Hit
ATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGA	11	0.27596588058203714	No Hit
ATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAG	11	0.27596588058203714	No Hit
CATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGA	11	0.27596588058203714	No Hit
CGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTT	11	0.27596588058203714	No Hit
TGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCC	11	0.27596588058203714	No Hit
GATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCAT	11	0.27596588058203714	No Hit
GACACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACT	11	0.27596588058203714	No Hit
AGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTT	11	0.27596588058203714	No Hit
GCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCA	10	0.2508780732563974	No Hit
ATTTCGCAGAGTTGAAGGTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAG	10	0.2508780732563974	No Hit
GGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAA	10	0.2508780732563974	No Hit
GCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTT	10	0.2508780732563974	No Hit
CTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAA	10	0.2508780732563974	No Hit
CAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGG	10	0.2508780732563974	No Hit
CTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTA	10	0.2508780732563974	No Hit
TTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGG	10	0.2508780732563974	No Hit
AAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGA	10	0.2508780732563974	No Hit
CGCTGTGCCAGAAACCCTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGA	10	0.2508780732563974	No Hit
CGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCT	10	0.2508780732563974	No Hit
CAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCC	10	0.2508780732563974	No Hit
TGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACG	10	0.2508780732563974	No Hit
GCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCA	10	0.2508780732563974	No Hit
ATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGG	9	0.22579026593075763	No Hit
AAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGC	9	0.22579026593075763	No Hit
GCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACA	9	0.22579026593075763	No Hit
AGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCC	9	0.22579026593075763	No Hit
GAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGC	9	0.22579026593075763	No Hit
ATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTT	9	0.22579026593075763	No Hit
ATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAG	9	0.22579026593075763	No Hit
TGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGC	9	0.22579026593075763	No Hit
CAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTG	9	0.22579026593075763	No Hit
AGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGA	9	0.22579026593075763	No Hit
GAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCT	9	0.22579026593075763	No Hit
CGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGA	9	0.22579026593075763	No Hit
GTTGAAGGTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAA	9	0.22579026593075763	No Hit
CAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTC	9	0.22579026593075763	No Hit
CGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAA	9	0.22579026593075763	No Hit
AGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATT	9	0.22579026593075763	No Hit
GTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGA	9	0.22579026593075763	No Hit
CTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAGCGCCTGAG	9	0.22579026593075763	No Hit
CAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTT	8	0.2007024586051179	No Hit
GTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCA	8	0.2007024586051179	No Hit
GAAACCCTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAGCG	8	0.2007024586051179	No Hit
TCTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATA	8	0.2007024586051179	No Hit
CACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTAT	8	0.2007024586051179	No Hit
AGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAAT	8	0.2007024586051179	No Hit
CGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTG	8	0.2007024586051179	No Hit
CTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAA	8	0.2007024586051179	No Hit
GGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGG	8	0.2007024586051179	No Hit
CTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAA	8	0.2007024586051179	No Hit
CAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGG	8	0.2007024586051179	No Hit
CTAATTCATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAA	8	0.2007024586051179	No Hit
GCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGG	8	0.2007024586051179	No Hit
GGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCAGAA	8	0.2007024586051179	No Hit
GACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAAC	8	0.2007024586051179	No Hit
GCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTA	8	0.2007024586051179	No Hit
CGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCC	8	0.2007024586051179	No Hit
CACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATG	7	0.17561465127947817	No Hit
AGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCTC	7	0.17561465127947817	No Hit
CCCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCA	7	0.17561465127947817	No Hit
TATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTT	7	0.17561465127947817	No Hit
ATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATT	7	0.17561465127947817	No Hit
CACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGAT	7	0.17561465127947817	No Hit
GTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGA	7	0.17561465127947817	No Hit
CAGAAACCCTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAG	7	0.17561465127947817	No Hit
GAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTATG	7	0.17561465127947817	No Hit
GTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAG	7	0.17561465127947817	No Hit
AATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGCATGGA	7	0.17561465127947817	No Hit
GTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACT	7	0.17561465127947817	No Hit
CTTCCCCACGAGGTGGGATGAAGAAAAAGACAACTCACTTTGTAGAAGGT	7	0.17561465127947817	No Hit
ATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCT	7	0.17561465127947817	No Hit
GCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATGT	7	0.17561465127947817	No Hit
AAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTA	7	0.17561465127947817	No Hit
CAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATG	7	0.17561465127947817	No Hit
TGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATA	7	0.17561465127947817	No Hit
CATGGAGGCTGTTCCAGAGAAGAAAAAGAAGGTTGCCACTGTGCCAGGAA	7	0.17561465127947817	No Hit
ATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACG	6	0.15052684395383845	No Hit
CCTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAGCGCCTGA	6	0.15052684395383845	No Hit
TAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTG	6	0.15052684395383845	No Hit
GAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTG	6	0.15052684395383845	No Hit
CAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAG	6	0.15052684395383845	No Hit
AAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGT	6	0.15052684395383845	No Hit
TAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACC	6	0.15052684395383845	No Hit
GGAACCCTTAAGAAAAAGGTTCCTGCTGGGCCAAAAACTCTCAAGAAAAA	6	0.15052684395383845	No Hit
TGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGAC	6	0.15052684395383845	No Hit
TCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCT	6	0.15052684395383845	No Hit
GCAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCG	6	0.15052684395383845	No Hit
AGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCTAT	6	0.15052684395383845	No Hit
CAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAA	6	0.15052684395383845	No Hit
AACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGC	6	0.15052684395383845	No Hit
CTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGC	6	0.15052684395383845	No Hit
AATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCA	6	0.15052684395383845	No Hit
GGTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAA	6	0.15052684395383845	No Hit
ACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCC	6	0.15052684395383845	No Hit
CTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCT	6	0.15052684395383845	No Hit
GTGGGATGAAGAAAAAGACAACTCACTTTGTAGAAGGTGGAGATGCTGGC	6	0.15052684395383845	No Hit
CTCACTTTGTAGAAGGTGGAGATGCTGGCAACAGGGAAGACCAGATAAAC	6	0.15052684395383845	No Hit
AGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAG	6	0.15052684395383845	No Hit
GTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAA	6	0.15052684395383845	No Hit
TCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTTC	6	0.15052684395383845	No Hit
CTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACGGATGAACTAA	5	0.1254390366281987	No Hit
GGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAGATCTTCAATGGCA	5	0.1254390366281987	No Hit
AAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATC	5	0.1254390366281987	No Hit
TTCGGATGGCCAGGATGGCAAGGAAAGCTGGCAACTTCTATGTGCCCGCA	5	0.1254390366281987	No Hit
GAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTTCGTCTTCGACAG	5	0.1254390366281987	No Hit
AGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATG	5	0.1254390366281987	No Hit
GAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGG	5	0.1254390366281987	No Hit
CGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTG	5	0.1254390366281987	No Hit
TGTCATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGG	5	0.1254390366281987	No Hit
CATGAGATCTATACAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCT	5	0.1254390366281987	No Hit
GACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCT	5	0.1254390366281987	No Hit
GAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAGCGCCTGAGGAAGA	5	0.1254390366281987	No Hit
GGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAAGAAAAAGACAACT	5	0.1254390366281987	No Hit
GGTCTCTTGGTAAGTTTGGCATCATCTGCATGGAGGATCTAATTCATGAG	5	0.1254390366281987	No Hit
TGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTG	5	0.1254390366281987	No Hit
CGCAGAGTTGAAGGTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACAC	5	0.1254390366281987	No Hit
GGGAAGACCAGATAAACAGGCTTATTAGACGGATGAACTAAGGTGTCACC	5	0.1254390366281987	No Hit
GCTTCAATTAACATGCTGCGGATTGTGGAGCCATACATTGCATGGGGGTA	5	0.1254390366281987	No Hit
GGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATC	5	0.1254390366281987	No Hit
CAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAG	5	0.1254390366281987	No Hit
GGTGGAGATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACG	5	0.1254390366281987	No Hit
AGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAG	5	0.1254390366281987	No Hit
AATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGAT	5	0.1254390366281987	No Hit
GGGTACCCCAACCTGAAGTCAGTAAACGAGCTCATCTACAAGCGAGGCTA	5	0.1254390366281987	No Hit
AAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCAT	5	0.1254390366281987	No Hit
AGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGG	5	0.1254390366281987	No Hit
GCTGCGGATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGT	5	0.1254390366281987	No Hit
AAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGG	5	0.1254390366281987	No Hit
TGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGG	5	0.1254390366281987	No Hit
GCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATAATTCCTTG	5	0.1254390366281987	No Hit
ATCAGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCT	5	0.1254390366281987	No Hit
AAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGA	5	0.1254390366281987	No Hit
TCGAAAGGCAAGGAGGAAGCTCATCTATGAGAAGGCAAAGCACTATCACA	5	0.1254390366281987	No Hit
CAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTC	5	0.1254390366281987	No Hit
AATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGATGAA	5	0.1254390366281987	No Hit
AGAATTCGAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCA	5	0.1254390366281987	No Hit
GCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGG	5	0.1254390366281987	No Hit
GTCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGA	5	0.1254390366281987	No Hit
TGGAGATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACGGA	5	0.1254390366281987	No Hit
GCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGGAT	5	0.1254390366281987	No Hit
AAGCACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGAT	4	0.10035122930255895	No Hit
GCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATT	4	0.10035122930255895	No Hit
ATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAACGCTT	4	0.10035122930255895	No Hit
CTCAAGAAAAAGGTTCCCGCTGTGCCAGAAACCCTCAAGAAAAAGCGAAG	4	0.10035122930255895	No Hit
GAGGTATCAATGGAGTAAGCCCAAAGGTTCGTAAGGTTCTGCAGCTCCTT	4	0.10035122930255895	No Hit
GATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGC	4	0.10035122930255895	No Hit
CGAAGGAATTTCGCAGAGTTGAAGGTGAAGCGCCTGAGGAAGAAGTTTGC	4	0.10035122930255895	No Hit
AGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGGTATCAATGGAGTAA	4	0.10035122930255895	No Hit
AAGCAAATAACTTCCTGTGGCCCTTCAAGTTATCTTCCCCACGAGGTGGG	4	0.10035122930255895	No Hit
GCCAGAAACCCTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGA	4	0.10035122930255895	No Hit
GATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGACGGATGAA	4	0.10035122930255895	No Hit
CACTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGC	4	0.10035122930255895	No Hit
TGTTAAGCTCAACAAGGCTTCAATTAACATGCTGCGGATTGTGGAGCCAT	4	0.10035122930255895	No Hit
ATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCAGG	4	0.10035122930255895	No Hit
AAACCCTCAAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAGCGC	4	0.10035122930255895	No Hit
CGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAGTTAT	4	0.10035122930255895	No Hit
AAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAA	4	0.10035122930255895	No Hit
ATCTTCAATGGCACCTTTGTTAAGCTCAACAAGGCTTCAATTAACATGCT	4	0.10035122930255895	No Hit
CAGTCGGGAAACGCTTCAAGGAAGCAAATAACTTCCTGTGGCCCTTCAAG	4	0.10035122930255895	No Hit
CATCTATGAGAAGGCAAAGCACTATCACAAGGAGTACAGGCAGATGTACC	4	0.10035122930255895	No Hit
TCAGTAAACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAA	4	0.10035122930255895	No Hit
GAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGC	4	0.10035122930255895	No Hit
GAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAAACGAGCT	4	0.10035122930255895	No Hit
GCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAG	4	0.10035122930255895	No Hit
GAAAGCTGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCA	4	0.10035122930255895	No Hit
AAGGTGGAGATGCTGGCAACAGGGAAGACCAGATAAACAGGCTTATTAGA	4	0.10035122930255895	No Hit
GATGTACCGCACTGAGATTCGGATGGCCAGGATGGCAAGGAAAGCTGGCA	4	0.10035122930255895	No Hit
TGGCATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGA	4	0.10035122930255895	No Hit
CCTTCGTCTTCGACAGATCTTCAATGGCACCTTTGTTAAGCTCAACAAGG	4	0.10035122930255895	No Hit
CCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGC	4	0.10035122930255895	No Hit
GGAAGACCAGATAAACAGGCTTATTAGACGGATGAACTAAGGTGTCACCC	4	0.10035122930255895	No Hit
TGGCAACTTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAA	4	0.10035122930255895	No Hit
GATTGTGGAGCCATACATTGCATGGGGGTACCCCAACCTGAAGTCAGTAA	4	0.10035122930255895	No Hit
GGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATC	4	0.10035122930255895	No Hit
AAAACTCTCAAGAAAAAGGTTCCCGCTGTGCCAGAAACCCTCAAGAAAAA	4	0.10035122930255895	No Hit
CATCATCTGCATGGAGGATCTAATTCATGAGATCTATACAGTCGGGAAAC	4	0.10035122930255895	No Hit
GTAAGAAGTTAGTGTTGTTTTTTGAGACAGAATCTATTTCTGGCAATCGC	4	0.10035122930255895	No Hit
CAAGAAAAAGGTTCCCGCTGTGCCAGAAACCCTCAAGAAAAAGCGAAGGA	4	0.10035122930255895	No Hit
CAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATCATCTGC	4	0.10035122930255895	No Hit
CAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGATTGCCTTGACAGATA	4	0.10035122930255895	No Hit
AACGAGCTCATCTACAAGCGAGGCTACGGCAAAATCAACAAGAAGCGGAT	4	0.10035122930255895	No Hit
TTCTATGTGCCCGCAGAACCAAAGCTGGCCTTTGTCATCAGAATTCGAGG	4	0.10035122930255895	No Hit
CTATCACAAGGAGTACAGGCAGATGTACCGCACTGAGATTCGGATGGCCA	4	0.10035122930255895	No Hit
GAAGCGGATTGCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTA	4	0.10035122930255895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.012543903662819869	0.0	0.0	0.0	0.0
82-83	0.2007024586051179	0.0	0.0	0.0	0.0
84-85	0.8278976417461114	0.0	0.0	0.0	0.0
86-87	1.9317611640742598	0.0	0.0	0.0	0.0
88-89	2.7972905168088307	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 7 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 2 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 6 READS because READLEN < 1
Read 457 spots for SRR25558503.sra
Written 457 spots for SRR25558503.sra
Rejected 5 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 6 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 7 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 5 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 4 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 3 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 2 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 3 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 1 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 3 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 1 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
Rejected 2 READS because READLEN < 1
Read 448 spots for SRR25558503.sra
Written 448 spots for SRR25558503.sra
SRR ids: ['SRR25558503.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__wb_e8du
SRR25558503.sra spots: 7177
blocks: [[1, 448], [449, 896], [897, 1344], [1345, 1792], [1793, 2240], [2241, 2688], [2689, 3136], [3137, 3584], [3585, 4032], [4033, 4480], [4481, 4928], [4929, 5376], [5377, 5824], [5825, 6272], [6273, 6720], [6721, 7177]]
SRR25558503 file size 1
SRR25558503 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25558503 SRR25558503_1.fastq SRR25558503_2.fastq
Input file:	SRR25558503_1.fastq
Paired file:	SRR25558503_2.fastq
trimmed:	SRR25558503-trimmed-pair1.fastq, SRR25558503-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 15:30:23 2026 >> started

Sun May 24 15:30:23 2026 >> done (0.016s)
7148 read pairs processed; of these:
  10 ( 0.14%) short read pairs filtered out after trimming by size control
   6 ( 0.08%) empty read pairs filtered out after trimming by size control
7132 (99.78%) read pairs available; of these:
 988 (13.85%) trimmed read pairs available after processing
6144 (86.15%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 61	   2	  0.03%
 62	   1	  0.01%
 63	   0	  0.00%
 64	   0	  0.00%
 65	   0	  0.00%
 66	   1	  0.01%
 67	   0	  0.00%
 68	   0	  0.00%
 69	   0	  0.00%
 70	   0	  0.00%
 71	   0	  0.00%
 72	   2	  0.03%
 73	   1	  0.01%
 74	   3	  0.04%
 75	   0	  0.00%
 76	   0	  0.00%
 77	   1	  0.01%
 78	   0	  0.00%
 79	   0	  0.00%
 80	   4	  0.06%
 81	   2	  0.03%
 82	  17	  0.24%
 83	  10	  0.14%
 84	  17	  0.24%
 85	  29	  0.41%
 86	  26	  0.36%
 87	  17	  0.24%
 88	  26	  0.36%
 89	  30	  0.42%
 90	  28	  0.39%
 91	  40	  0.56%
 92	  38	  0.53%
 93	  41	  0.57%
 94	  44	  0.62%
 95	  42	  0.59%
 96	  38	  0.53%
 97	  59	  0.83%
 98	  69	  0.97%
 99	 101	  1.42%
100	 299	  4.19%
101	6144	 86.15%
7132 reads passed initial QC


criterion=sequence-density
sequence-density=8.78
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=4
prefix-density=8.85
prefix-fanout=2.0
sequence=GGAACCTTTTTCTT


criterion=fanout-score
sequence-density=8.61
sequence-density-rank=2
fanout-score=8.44
fanout-score-rank=1
prefix-density=23.01
prefix-fanout=3.2
sequence=CTTTTTCTTGAG


criterion=sequence-density
sequence-density=16.92
sequence-density-rank=1
fanout-score=7.10
fanout-score-rank=1
prefix-density=37.06
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=16.92
sequence-density-rank=1
fanout-score=7.10
fanout-score-rank=1
prefix-density=37.06
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x GGAACCTTTTTCTT -y AAGAAAAAGGTTCC -o SRR25558503 SRR25558503_1.fastq SRR25558503_2.fastq
Input file:	SRR25558503_1.fastq
Paired file:	SRR25558503_2.fastq
trimmed:	SRR25558503-trimmed-pair1.fastq, SRR25558503-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GGAACCTTTTTCTT
-- paired 3' end adapter sequence (-y):	AAGAAAAAGGTTCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 15:30:24 2026 >> started

Sun May 24 15:30:24 2026 >> done (0.013s)
6035 read pairs processed; of these:
   3 ( 0.05%) short read pairs filtered out after trimming by size control
   6 ( 0.10%) empty read pairs filtered out after trimming by size control
6026 (99.85%) read pairs available; of these:
  12 ( 0.20%) trimmed read pairs available after processing
6014 (99.80%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 61	   2	  0.03%
 62	   0	  0.00%
 63	   0	  0.00%
 64	   0	  0.00%
 65	   0	  0.00%
 66	   1	  0.02%
 67	   0	  0.00%
 68	   0	  0.00%
 69	   0	  0.00%
 70	   0	  0.00%
 71	   0	  0.00%
 72	   2	  0.03%
 73	   1	  0.02%
 74	   2	  0.03%
 75	   0	  0.00%
 76	   0	  0.00%
 77	   1	  0.02%
 78	   0	  0.00%
 79	   0	  0.00%
 80	   1	  0.02%
 81	   2	  0.03%
 82	  14	  0.23%
 83	   8	  0.13%
 84	  15	  0.25%
 85	  22	  0.37%
 86	  23	  0.38%
 87	  15	  0.25%
 88	  24	  0.40%
 89	  27	  0.45%
 90	  25	  0.41%
 91	  39	  0.65%
 92	  35	  0.58%
 93	  36	  0.60%
 94	  37	  0.61%
 95	  33	  0.55%
 96	  34	  0.56%
 97	  50	  0.83%
 98	  58	  0.96%
 99	  91	  1.51%
100	 256	  4.25%
101	5172	 85.83%


criterion=sequence-density
sequence-density=8.63
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=4
prefix-density=8.76
prefix-fanout=2.0
sequence=GGAACCTTTTTCTT


criterion=fanout-score
sequence-density=8.55
sequence-density-rank=2
fanout-score=8.42
fanout-score-rank=1
prefix-density=22.81
prefix-fanout=3.2
sequence=CTTTTTCTTGAG


criterion=sequence-density
sequence-density=16.75
sequence-density-rank=1
fanout-score=7.14
fanout-score-rank=1
prefix-density=36.85
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=16.75
sequence-density-rank=1
fanout-score=7.14
fanout-score-rank=1
prefix-density=36.85
prefix-fanout=3.2
sequence=AAGAAAAAGGTTCC
SRR25558503 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 15:30:45
                             Started mapping on |	May 24 15:30:45
                                    Finished on |	May 24 15:30:47
       Mapping speed, Million of reads per hour |	12.82

                          Number of input reads |	7123
                      Average input read length |	200
                                    UNIQUE READS:
                   Uniquely mapped reads number |	6434
                        Uniquely mapped reads % |	90.33%
                          Average mapped length |	199.27
                       Number of splices: Total |	7742
            Number of splices: Annotated (sjdb) |	7732
                       Number of splices: GT/AG |	7739
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	3
                      Mismatch rate per base, % |	0.10%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.45
                        Insertion rate per base |	0.00%
                       Insertion average length |	9.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	553
             % of reads mapped to multiple loci |	7.76%
        Number of reads mapped to too many loci |	7
             % of reads mapped to too many loci |	0.10%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.81%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	140	140	140
N_multimapping	553	553	553
N_noFeature	36	6431	38
N_ambiguous	78	0	77
UnstrandedReadsAssigned:6320 PositiveStrandReadsAssigned:3 NegativeStrandReadsAssigned:6319
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR25558503 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558503-trimmed-pair1.fastq
                             SRR25558503-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 7,123 reads, 6,847 reads pseudoaligned
[quant] estimated average fragment length: 138.836
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 90 rounds

 109283 SRR25558503.ke.tsv
  52636 SRR25558503.se.tsv
 161919 total
==> SRR25558503.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25558503.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558503 completed mapping pipeline successfully
