Starting /dee2/code/volunteer_pipeline.sh SRR25558762
    current disk space = 1139578520
    free memory = 194004864 
SRR25558762 SRAfilesize
c989d01d921c972787334e0b07a22f97  SRR25558762.sra
SRR25558762.sra file validated
SRR25558762 is paired end
SRR25558762 is conventional basespace
SRR25558762 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558762_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.432	37.0	37.0	37.0	37.0	37.0
2	36.342	37.0	37.0	37.0	37.0	37.0
3	36.4415	37.0	37.0	37.0	37.0	37.0
4	36.394	37.0	37.0	37.0	37.0	37.0
5	36.5095	37.0	37.0	37.0	37.0	37.0
6	36.558	37.0	37.0	37.0	37.0	37.0
7	36.501	37.0	37.0	37.0	37.0	37.0
8	36.4265	37.0	37.0	37.0	37.0	37.0
9	36.462	37.0	37.0	37.0	37.0	37.0
10-14	36.497400000000006	37.0	37.0	37.0	37.0	37.0
15-19	36.509100000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.5071	37.0	37.0	37.0	37.0	37.0
25-29	36.44350000000001	37.0	37.0	37.0	37.0	37.0
30-34	36.430099999999996	37.0	37.0	37.0	37.0	37.0
35-39	36.388600000000004	37.0	37.0	37.0	37.0	37.0
40-44	36.3693	37.0	37.0	37.0	37.0	37.0
45-49	36.3763	37.0	37.0	37.0	37.0	37.0
50-54	36.2971	37.0	37.0	37.0	37.0	37.0
55-59	36.2713	37.0	37.0	37.0	37.0	37.0
60-64	36.3088	37.0	37.0	37.0	37.0	37.0
65-69	36.253	37.0	37.0	37.0	37.0	37.0
70-74	36.2946	37.0	37.0	37.0	37.0	37.0
75-79	36.261900000000004	37.0	37.0	37.0	37.0	37.0
80-84	36.2338	37.0	37.0	37.0	37.0	37.0
85-89	36.2104	37.0	37.0	37.0	37.0	37.0
90-94	36.160000000000004	37.0	37.0	37.0	37.0	37.0
95-99	36.13590000000001	37.0	37.0	37.0	37.0	37.0
100-104	36.062799999999996	37.0	37.0	37.0	37.0	37.0
105-109	36.0846	37.0	37.0	37.0	37.0	37.0
110-114	36.160399999999996	37.0	37.0	37.0	37.0	37.0
115-119	36.05550000000001	37.0	37.0	37.0	37.0	37.0
120-124	36.0739	37.0	37.0	37.0	37.0	37.0
125-129	35.9195	37.0	37.0	37.0	37.0	37.0
130-134	35.9262	37.0	37.0	37.0	37.0	37.0
135-139	35.9579	37.0	37.0	37.0	37.0	37.0
140-144	35.8859	37.0	37.0	37.0	37.0	37.0
145-149	35.9533	37.0	37.0	37.0	37.0	37.0
150	35.9455	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	1.0
27	11.0
28	14.0
29	21.0
30	33.0
31	37.0
32	51.0
33	86.0
34	119.0
35	279.0
36	3056.0
37	291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.325	16.275000000000002	17.95	37.45
2	22.058087130696045	25.81372058087131	34.87731597396094	17.250876314471707
3	26.025	30.099999999999998	22.7	21.175
4	25.575	35.975	20.349999999999998	18.099999999999998
5	23.0	38.05	21.625	17.325
6	18.224999999999998	38.775	22.875	20.125
7	17.65	17.075000000000003	41.875	23.400000000000002
8	19.8	23.599999999999998	29.849999999999998	26.75
9	22.1	24.025	27.6	26.275
10-14	21.935	30.014999999999997	25.44	22.61
15-19	22.17	25.97	27.52	24.34
20-24	21.145	28.62	28.4	21.834999999999997
25-29	23.13	27.0	26.35	23.52
30-34	22.395	28.425	26.405	22.775000000000002
35-39	22.125	27.865000000000002	27.150000000000002	22.86
40-44	23.474999999999998	27.279999999999998	26.724999999999998	22.52
45-49	21.17	29.345	26.939999999999998	22.545
50-54	22.61	29.13	27.275	20.985
55-59	20.625	27.43	28.735	23.21
60-64	23.119999999999997	27.625	27.12	22.134999999999998
65-69	21.205	27.97	29.080000000000002	21.745
70-74	21.83	27.644999999999996	28.58	21.945
75-79	23.13	28.655	27.16	21.055
80-84	21.625	28.1	28.9	21.375
85-89	22.009999999999998	28.105000000000004	28.970000000000002	20.915
90-94	22.5	29.285	26.51	21.705
95-99	21.475	29.365000000000002	28.575	20.585
100-104	20.44	28.88	28.060000000000002	22.62
105-109	22.1	27.79	28.43	21.68
110-114	21.295	27.915	29.065	21.725
115-119	21.365000000000002	28.17	28.139999999999997	22.325
120-124	22.205	27.245	29.020000000000003	21.529999999999998
125-129	21.97	28.065	28.955	21.01
130-134	21.34	28.17	28.794999999999998	21.695
135-139	22.065	27.355	29.044999999999998	21.535
140-144	21.16	29.5	27.450000000000003	21.89
145-149	21.759999999999998	28.9	26.755000000000003	22.585
150	20.8	31.25	26.400000000000002	21.55
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.5
9	1.0
10	0.5
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	1.0
20	1.0
21	0.5
22	2.0
23	1.5
24	1.0
25	3.0
26	8.0
27	29.5
28	77.5
29	81.0
30	51.0
31	36.5
32	34.0
33	55.0
34	50.0
35	42.5
36	65.0
37	86.0
38	96.5
39	145.5
40	192.0
41	199.5
42	200.0
43	197.5
44	236.0
45	224.5
46	235.5
47	264.0
48	238.5
49	192.5
50	129.5
51	113.0
52	110.5
53	114.0
54	99.0
55	71.0
56	51.0
57	33.5
58	24.5
59	16.0
60	12.0
61	9.5
62	7.5
63	10.0
64	13.5
65	19.5
66	19.5
67	17.5
68	14.5
69	9.0
70	14.0
71	16.0
72	10.5
73	6.5
74	1.5
75	1.0
76	1.5
77	0.5
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.543743078626804	24.175
2	22.314507198228128	20.150000000000002
3	9.246954595791806	12.525
4	5.92469545957918	10.7
5	2.7131782945736433	6.125
6	1.8826135105204873	5.1
7	0.9413067552602437	2.9749999999999996
8	1.1627906976744187	4.2
9	0.5537098560354374	2.25
>10	1.6611295681063125	10.375
>50	0.05537098560354374	1.425
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	57	1.425	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	35	0.8750000000000001	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	21	0.525	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	20	0.5	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	17	0.42500000000000004	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	17	0.42500000000000004	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	16	0.4	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	16	0.4	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	15	0.375	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	14	0.35000000000000003	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	14	0.35000000000000003	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	14	0.35000000000000003	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	14	0.35000000000000003	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	14	0.35000000000000003	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	13	0.325	No Hit
GTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACT	13	0.325	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	13	0.325	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	13	0.325	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	12	0.3	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	11	0.27499999999999997	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	11	0.27499999999999997	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	11	0.27499999999999997	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	11	0.27499999999999997	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	10	0.25	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	10	0.25	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	10	0.25	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	10	0.25	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	10	0.25	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	10	0.25	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	10	0.25	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	10	0.25	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	9	0.22499999999999998	No Hit
TTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTC	9	0.22499999999999998	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	9	0.22499999999999998	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	9	0.22499999999999998	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	9	0.22499999999999998	No Hit
GAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCA	9	0.22499999999999998	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	9	0.22499999999999998	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	9	0.22499999999999998	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	9	0.22499999999999998	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	9	0.22499999999999998	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	8	0.2	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	8	0.2	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	8	0.2	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	8	0.2	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	8	0.2	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	8	0.2	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	8	0.2	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	8	0.2	No Hit
CTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTT	8	0.2	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	8	0.2	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	8	0.2	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	8	0.2	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	8	0.2	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	8	0.2	No Hit
GAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACT	8	0.2	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	8	0.2	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	8	0.2	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	8	0.2	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	8	0.2	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	8	0.2	No Hit
GAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAG	8	0.2	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	7	0.17500000000000002	No Hit
AGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGT	7	0.17500000000000002	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	7	0.17500000000000002	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	7	0.17500000000000002	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	7	0.17500000000000002	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	7	0.17500000000000002	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	7	0.17500000000000002	No Hit
CGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTT	7	0.17500000000000002	No Hit
CTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGG	7	0.17500000000000002	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	7	0.17500000000000002	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	7	0.17500000000000002	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	7	0.17500000000000002	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	7	0.17500000000000002	No Hit
CTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCGATCAA	7	0.17500000000000002	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	7	0.17500000000000002	No Hit
GCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAG	7	0.17500000000000002	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	7	0.17500000000000002	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	6	0.15	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	6	0.15	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	6	0.15	No Hit
GCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATA	6	0.15	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	6	0.15	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	6	0.15	No Hit
GATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACA	6	0.15	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	6	0.15	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	6	0.15	No Hit
ATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCT	6	0.15	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	6	0.15	No Hit
TCAGAATATACTAAGTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTT	6	0.15	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	6	0.15	No Hit
CTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTG	6	0.15	No Hit
TAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGA	6	0.15	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	6	0.15	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	6	0.15	No Hit
TGACCATAGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACA	6	0.15	No Hit
ATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCC	6	0.15	No Hit
AAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACA	6	0.15	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	6	0.15	No Hit
TGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAAT	6	0.15	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	6	0.15	No Hit
CAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCA	6	0.15	No Hit
CTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAA	6	0.15	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	6	0.15	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	6	0.15	No Hit
TGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTT	6	0.15	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	6	0.15	No Hit
AGAATATACTAAGTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGT	6	0.15	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	6	0.15	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	6	0.15	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	6	0.15	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	6	0.15	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	5	0.125	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	5	0.125	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	5	0.125	No Hit
CTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTCGATTA	5	0.125	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	5	0.125	No Hit
CCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGA	5	0.125	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	5	0.125	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	5	0.125	No Hit
TTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTG	5	0.125	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	5	0.125	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	5	0.125	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	5	0.125	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	5	0.125	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	5	0.125	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	5	0.125	No Hit
TGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTT	5	0.125	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	5	0.125	No Hit
GCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	5	0.125	No Hit
TAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGAC	5	0.125	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	5	0.125	No Hit
GTCCACTGTTGTACAACCACTAAGTGAGGTTAAATCACTTGCTTCAGGCA	5	0.125	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	5	0.125	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	5	0.125	No Hit
TGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAG	5	0.125	No Hit
GAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTT	5	0.125	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	5	0.125	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	5	0.125	No Hit
CCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAA	5	0.125	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	5	0.125	No Hit
AATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACC	5	0.125	No Hit
CTGAATTGTATAATACAAATATATGCATAGACACTTAAAAGGATCATATA	5	0.125	No Hit
TGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAG	5	0.125	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	5	0.125	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	5	0.125	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	5	0.125	No Hit
CTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAATGATA	5	0.125	No Hit
ATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCA	5	0.125	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	5	0.125	No Hit
GGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGT	5	0.125	No Hit
AGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTA	5	0.125	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	5	0.125	No Hit
CCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGC	5	0.125	No Hit
CAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACT	5	0.125	No Hit
TGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAG	5	0.125	No Hit
TAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGC	5	0.125	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	5	0.125	No Hit
CAGGAAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	10	0.006973645	144.0	6
TGGTAAC	10	0.006973645	144.0	1
TAACAGA	10	0.006973645	144.0	4
CAGACCC	10	0.006973645	144.0	7
AACAGAC	10	0.006973645	144.0	5
AGACCCA	10	0.006973645	144.0	8
GTAACAG	10	0.006973645	144.0	3
GGTAACA	10	0.006973645	144.0	2
CAAGAAT	30	0.0018473949	72.0	1
CCATGTG	30	0.0018473949	72.0	8
AGAATCC	30	0.0018473949	72.0	3
GAATCCA	30	0.0018473949	72.0	4
TCCATGT	30	0.0018473949	72.0	7
CATGTGC	30	0.0018473949	72.0	9
AATCCAT	35	0.0034045284	61.714283	5
ATCCATG	35	0.0034045284	61.714283	6
AAGAATC	45	0.009205684	48.0	2
>>END_MODULE
SRR25558762 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558762_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3285	37.0	37.0	37.0	37.0	37.0
2	36.314	37.0	37.0	37.0	37.0	37.0
3	36.38	37.0	37.0	37.0	37.0	37.0
4	36.3445	37.0	37.0	37.0	37.0	37.0
5	36.3285	37.0	37.0	37.0	37.0	37.0
6	36.2955	37.0	37.0	37.0	37.0	37.0
7	36.2545	37.0	37.0	37.0	37.0	37.0
8	36.3445	37.0	37.0	37.0	37.0	37.0
9	36.3805	37.0	37.0	37.0	37.0	37.0
10-14	36.2866	37.0	37.0	37.0	37.0	37.0
15-19	36.326	37.0	37.0	37.0	37.0	37.0
20-24	36.2721	37.0	37.0	37.0	37.0	37.0
25-29	36.1887	37.0	37.0	37.0	37.0	37.0
30-34	36.224000000000004	37.0	37.0	37.0	37.0	37.0
35-39	36.1877	37.0	37.0	37.0	37.0	37.0
40-44	36.1707	37.0	37.0	37.0	37.0	37.0
45-49	36.1192	37.0	37.0	37.0	37.0	37.0
50-54	36.0856	37.0	37.0	37.0	37.0	37.0
55-59	36.1474	37.0	37.0	37.0	37.0	37.0
60-64	36.1149	37.0	37.0	37.0	37.0	37.0
65-69	36.0695	37.0	37.0	37.0	37.0	37.0
70-74	35.9778	37.0	37.0	37.0	37.0	37.0
75-79	36.0221	37.0	37.0	37.0	37.0	37.0
80-84	35.923	37.0	37.0	37.0	37.0	37.0
85-89	35.9406	37.0	37.0	37.0	37.0	37.0
90-94	35.8885	37.0	37.0	37.0	37.0	37.0
95-99	35.761	37.0	37.0	37.0	37.0	37.0
100-104	35.7931	37.0	37.0	37.0	37.0	37.0
105-109	35.7321	37.0	37.0	37.0	37.0	37.0
110-114	35.7221	37.0	37.0	37.0	37.0	37.0
115-119	35.6242	37.0	37.0	37.0	37.0	37.0
120-124	35.4257	37.0	37.0	37.0	37.0	37.0
125-129	35.484300000000005	37.0	37.0	37.0	37.0	37.0
130-134	35.4707	37.0	37.0	37.0	37.0	37.0
135-139	35.5113	37.0	37.0	37.0	34.6	37.0
140-144	35.43769999999999	37.0	37.0	37.0	37.0	37.0
145-149	35.2321	37.0	37.0	37.0	27.4	37.0
150	35.0365	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	1.0
21	1.0
22	2.0
23	2.0
24	4.0
25	5.0
26	4.0
27	10.0
28	20.0
29	25.0
30	19.0
31	50.0
32	69.0
33	93.0
34	176.0
35	642.0
36	2728.0
37	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.299999999999997	16.175	17.424999999999997	37.1
2	21.8	25.8	34.849999999999994	17.549999999999997
3	25.8	28.475	24.875	20.849999999999998
4	25.474999999999998	34.825	21.125	18.575
5	22.6	37.574999999999996	21.2	18.625
6	18.625	39.45	22.5	19.425
7	16.2	17.075000000000003	44.45	22.275
8	18.8	24.349999999999998	30.8	26.05
9	21.075	24.975	28.025	25.924999999999997
10-14	22.545	29.695	25.055	22.705000000000002
15-19	22.675	27.395000000000003	27.46	22.470000000000002
20-24	22.189999999999998	27.905	28.03	21.875
25-29	22.470000000000002	27.415	26.939999999999998	23.175
30-34	21.45	28.544999999999998	27.215	22.79
35-39	22.735	27.705000000000002	26.939999999999998	22.62
40-44	22.86	27.575	27.33	22.235
45-49	21.805	28.95	26.86	22.384999999999998
50-54	22.875	28.01	27.83	21.285
55-59	21.245	27.165	28.985	22.605
60-64	22.57	27.650000000000002	27.74	22.040000000000003
65-69	21.365000000000002	26.815	28.895	22.925
70-74	21.59	27.46	28.549999999999997	22.400000000000002
75-79	22.85	28.93	27.089999999999996	21.13
80-84	21.775	28.185	29.349999999999998	20.69
85-89	22.275	28.43	27.87	21.425
90-94	22.38	28.615000000000002	26.985	22.02
95-99	21.51	28.634999999999998	29.549999999999997	20.305
100-104	20.544999999999998	28.74	28.794999999999998	21.92
105-109	21.965	28.265	28.305000000000003	21.465
110-114	21.365000000000002	27.694999999999997	28.93	22.009999999999998
115-119	21.01	28.449999999999996	28.585	21.955
120-124	21.584999999999997	27.045	29.93	21.44
125-129	21.54	27.944999999999997	29.060000000000002	21.455
130-134	20.830000000000002	28.285	29.505	21.38
135-139	22.115000000000002	27.345000000000002	29.26	21.279999999999998
140-144	21.415	29.580000000000002	27.3	21.705
145-149	21.395	29.275000000000002	26.865	22.465
150	20.674999999999997	31.574999999999996	27.0	20.75
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.5
16	1.5
17	0.0
18	0.5
19	0.5
20	1.0
21	1.5
22	1.0
23	2.5
24	5.0
25	5.5
26	12.5
27	31.5
28	72.5
29	92.0
30	65.0
31	38.5
32	35.0
33	49.0
34	50.0
35	51.0
36	74.0
37	92.0
38	94.5
39	142.5
40	176.5
41	187.5
42	191.5
43	178.5
44	228.0
45	222.5
46	217.5
47	253.0
48	254.0
49	193.5
50	133.0
51	132.5
52	114.0
53	111.0
54	103.0
55	71.5
56	52.5
57	36.5
58	22.5
59	14.0
60	10.5
61	8.0
62	3.5
63	7.0
64	14.5
65	23.5
66	22.0
67	18.0
68	14.5
69	8.5
70	13.5
71	17.5
72	11.5
73	6.5
74	2.5
75	0.5
76	0.5
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.824999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.82431678750698	24.575
2	20.858895705521473	18.7
3	8.97936419408812	12.075
4	5.131065253764641	9.2
5	3.4021193530395983	7.625
6	2.230897936419409	6.0
7	1.6731734523145567	5.25
8	0.5577244841048522	2.0
9	0.4461795872838818	1.7999999999999998
>10	1.8404907975460123	11.3
>50	0.055772448410485224	1.4749999999999999
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	59	1.4749999999999999	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	27	0.675	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	25	0.625	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	23	0.575	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	20	0.5	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	18	0.44999999999999996	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	17	0.42500000000000004	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	17	0.42500000000000004	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	16	0.4	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	15	0.375	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	15	0.375	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	14	0.35000000000000003	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	14	0.35000000000000003	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	13	0.325	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	13	0.325	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	13	0.325	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	13	0.325	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	12	0.3	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	12	0.3	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	11	0.27499999999999997	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	11	0.27499999999999997	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	11	0.27499999999999997	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	11	0.27499999999999997	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	11	0.27499999999999997	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	10	0.25	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	10	0.25	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	10	0.25	No Hit
GTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTT	10	0.25	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	10	0.25	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	10	0.25	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	10	0.25	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	10	0.25	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	10	0.25	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	10	0.25	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	9	0.22499999999999998	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	9	0.22499999999999998	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	9	0.22499999999999998	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	9	0.22499999999999998	No Hit
CTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCC	9	0.22499999999999998	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	9	0.22499999999999998	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	9	0.22499999999999998	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	9	0.22499999999999998	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	8	0.2	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	8	0.2	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	8	0.2	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	8	0.2	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	8	0.2	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	8	0.2	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	8	0.2	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	8	0.2	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	8	0.2	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	8	0.2	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	7	0.17500000000000002	No Hit
TGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTC	7	0.17500000000000002	No Hit
CCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGA	7	0.17500000000000002	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	7	0.17500000000000002	No Hit
GCAAAATAAGATAACAAACAGTTTTATTACATCATTTGAAAATTACTAGT	7	0.17500000000000002	No Hit
TCCCTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGG	7	0.17500000000000002	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	7	0.17500000000000002	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	7	0.17500000000000002	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	7	0.17500000000000002	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	7	0.17500000000000002	No Hit
ATACAATTCAGAATATACTAAGTATTACAGTAGGTAAGTGAAGCAACTAG	7	0.17500000000000002	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	7	0.17500000000000002	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	7	0.17500000000000002	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	7	0.17500000000000002	No Hit
CAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCC	7	0.17500000000000002	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	7	0.17500000000000002	No Hit
GGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGG	7	0.17500000000000002	No Hit
CTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAA	7	0.17500000000000002	No Hit
GCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCT	7	0.17500000000000002	No Hit
GAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCA	7	0.17500000000000002	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	7	0.17500000000000002	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	7	0.17500000000000002	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	7	0.17500000000000002	No Hit
TTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATT	7	0.17500000000000002	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	7	0.17500000000000002	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	7	0.17500000000000002	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	7	0.17500000000000002	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	7	0.17500000000000002	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	7	0.17500000000000002	No Hit
CACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGA	7	0.17500000000000002	No Hit
CTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGT	6	0.15	No Hit
AAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAA	6	0.15	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	6	0.15	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	6	0.15	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	6	0.15	No Hit
TCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTC	6	0.15	No Hit
ATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGC	6	0.15	No Hit
GTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACT	6	0.15	No Hit
TAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAG	6	0.15	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	6	0.15	No Hit
CAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGC	6	0.15	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	6	0.15	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	6	0.15	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	6	0.15	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	6	0.15	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	6	0.15	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	6	0.15	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	6	0.15	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	6	0.15	No Hit
AGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTC	6	0.15	No Hit
AAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGT	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	6	0.15	No Hit
TGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAAC	6	0.15	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	6	0.15	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	6	0.15	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	6	0.15	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	6	0.15	No Hit
CAGACACTGCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAAC	6	0.15	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	6	0.15	No Hit
CTAAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTAT	6	0.15	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	6	0.15	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	6	0.15	No Hit
TGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATAT	6	0.15	No Hit
TCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGA	6	0.15	No Hit
CTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCAC	6	0.15	No Hit
GAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAG	6	0.15	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	6	0.15	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	6	0.15	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	6	0.15	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	5	0.125	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	5	0.125	No Hit
CTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGT	5	0.125	No Hit
GCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATA	5	0.125	No Hit
GCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGC	5	0.125	No Hit
CACAATCTCCATGGCACTGGAGAACGGAAATATCTCGATTAGCACTGTTG	5	0.125	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	5	0.125	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	5	0.125	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	5	0.125	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	5	0.125	No Hit
CTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTCGATTA	5	0.125	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	5	0.125	No Hit
ACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCACT	5	0.125	No Hit
TGGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATC	5	0.125	No Hit
CAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACA	5	0.125	No Hit
CATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTTCAGGTAAGA	5	0.125	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	5	0.125	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	5	0.125	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	5	0.125	No Hit
CCTACCTAACAGTTTTGTGTAATCCTCCAACCTCATCCTACCACTCTGGC	5	0.125	No Hit
CGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGG	5	0.125	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	5	0.125	No Hit
TCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCAC	5	0.125	No Hit
GCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGA	5	0.125	No Hit
TGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTT	5	0.125	No Hit
GTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACCCTCATG	5	0.125	No Hit
AAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAG	5	0.125	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	5	0.125	No Hit
TAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATG	5	0.125	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	5	0.125	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	5	0.125	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	5	0.125	No Hit
TGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTT	5	0.125	No Hit
CGCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGA	5	0.125	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	5	0.125	No Hit
GGATCATATATTTCCCATGAGGGTAACATCATTTAGTCTGTATCATTGGT	5	0.125	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	5	0.125	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	5	0.125	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	5	0.125	No Hit
CAATGATACAGACTAAATGATGTTACCCTCATGGGAAATATATGATCCTT	5	0.125	No Hit
ATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGA	5	0.125	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	5	0.125	No Hit
CTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGG	5	0.125	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	5	0.125	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	5	0.125	No Hit
GCTTAAAAGGATCATATATTTCCCATGAGGGTAACATCATTTAGTCTGTA	5	0.125	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	5	0.125	No Hit
GCATCATGCCTTCATAGATTTTGAAGGTTACATTGGCTGGATTTATCAAT	5	0.125	No Hit
GCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCAC	5	0.125	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	5	0.125	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	5	0.125	No Hit
CTATGGTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGT	5	0.125	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGC	5	0.125	No Hit
AATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGG	5	0.125	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	5	0.125	No Hit
AATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCAT	5	0.125	No Hit
TAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCTCA	5	0.125	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	5	0.125	No Hit
GTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGCATATATT	5	0.125	No Hit
GTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATAT	5	0.125	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAATCC	10	0.006973645	144.0	3
GAATCCA	10	0.006973645	144.0	4
>>END_MODULE
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
Read 5323493 spots for SRR25558762.sra
Written 5323493 spots for SRR25558762.sra
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
Read 5323491 spots for SRR25558762.sra
Written 5323491 spots for SRR25558762.sra
SRR ids: ['SRR25558762.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_mv58yjze
SRR25558762.sra spots: 42587930
blocks: [[1, 5323491], [5323492, 10646982], [10646983, 15970473], [15970474, 21293964], [21293965, 26617455], [26617456, 31940946], [31940947, 37264437], [37264438, 42587930]]
SRR25558762 file size 14368388
SRR25558762 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25558762 SRR25558762_1.fastq SRR25558762_2.fastq
Input file:	SRR25558762_1.fastq
Paired file:	SRR25558762_2.fastq
trimmed:	SRR25558762-trimmed-pair1.fastq, SRR25558762-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun May 24 14:54:02 2026 >> started

Sun May 24 14:57:07 2026 >> done (185.678s)
42587930 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       9 ( 0.00%) empty read pairs filtered out after trimming by size control
42587921 (100.00%) read pairs available; of these:
  428770 ( 1.01%) trimmed read pairs available after processing
42159151 (98.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 21	       1	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       2	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       1	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       1	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       1	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       1	  0.00%
 64	       0	  0.00%
 65	       3	  0.00%
 66	       0	  0.00%
 67	       1	  0.00%
 68	       3	  0.00%
 69	       5	  0.00%
 70	       5	  0.00%
 71	       6	  0.00%
 72	      11	  0.00%
 73	       1	  0.00%
 74	       7	  0.00%
 75	       2	  0.00%
 76	       5	  0.00%
 77	       4	  0.00%
 78	       4	  0.00%
 79	       6	  0.00%
 80	       6	  0.00%
 81	       9	  0.00%
 82	       5	  0.00%
 83	      12	  0.00%
 84	       7	  0.00%
 85	      12	  0.00%
 86	      15	  0.00%
 87	      18	  0.00%
 88	      19	  0.00%
 89	      28	  0.00%
 90	      14	  0.00%
 91	      19	  0.00%
 92	      21	  0.00%
 93	      33	  0.00%
 94	      27	  0.00%
 95	      33	  0.00%
 96	      36	  0.00%
 97	      29	  0.00%
 98	      43	  0.00%
 99	      39	  0.00%
100	      58	  0.00%
101	      72	  0.00%
102	      70	  0.00%
103	      74	  0.00%
104	      69	  0.00%
105	     103	  0.00%
106	      90	  0.00%
107	     103	  0.00%
108	     108	  0.00%
109	     106	  0.00%
110	     120	  0.00%
111	     118	  0.00%
112	     128	  0.00%
113	     144	  0.00%
114	     181	  0.00%
115	     202	  0.00%
116	     190	  0.00%
117	     194	  0.00%
118	     224	  0.00%
119	     227	  0.00%
120	     221	  0.00%
121	     267	  0.00%
122	     268	  0.00%
123	     292	  0.00%
124	     281	  0.00%
125	     321	  0.00%
126	     364	  0.00%
127	     363	  0.00%
128	     393	  0.00%
129	     404	  0.00%
130	     470	  0.00%
131	     450	  0.00%
132	     493	  0.00%
133	     472	  0.00%
134	     561	  0.00%
135	     570	  0.00%
136	     552	  0.00%
137	     708	  0.00%
138	     708	  0.00%
139	     717	  0.00%
140	     773	  0.00%
141	   38904	  0.09%
142	   40599	  0.10%
143	   41573	  0.10%
144	   43984	  0.10%
145	   45510	  0.11%
146	   48030	  0.11%
147	   50261	  0.12%
148	   52457	  0.12%
149	   54728	  0.13%
150	42159151	 98.99%
42587921 reads passed initial QC


criterion=sequence-density
sequence-density=1.64
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=38
prefix-density=1.62
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=166.90
fanout-score-rank=1
prefix-density=0.44
prefix-fanout=2.3
sequence=TTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGACACTTATATTTCGCTCTCACCTAATATCCACATTACTATGCCTGGAAGGCATAGTATTATCCTTATTTATTATAACTTCAGTAACTTCCCTAAACTCCAACTCCATAAGCTCCATACCAATCCCCATCACCATCTTAGTTTTCGCAGCCTGCGAAGCAGCTGTAGGACTAGCCCTACTAGTAAAAGTTTCAAACACGTACGGAACAGATTACGTCCAAAATCTCAACCTACTACAATGCTAAAAATTATTCTTCCCTC


criterion=sequence-density
sequence-density=1.61
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=40
prefix-density=1.59
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=44
fanout-score=154.79
fanout-score-rank=1
prefix-density=0.78
prefix-fanout=1.0
sequence=TGGTAAAAGCTAGCTTTTTAGAGGGAACAGCTTTTTAACAAGAAAACCCAAGCAACTGCTGCCATGGCGGGGAAGCCAGTCCTTCATTACTTTGATGGCAGGGGAAGAATGGAGCCTATCCGGTGGCTCTTGGCTGCAGCTGGTGTGGAGTTTGAAGAAAAATTTCTGAAAACTCGGGATGACCTGGCAAGGTTACGAAGTGATGGGAGTCTGATGTTCCAGCAAGTGCCCATGGTAGAGATCGACGGGATGAAACTGGTGCAGACCAAAGCCATTCTCAACTACATTGCCTCCAAATACAACCTCTATGGGAAGGACATGAAGGAGAGAGCCATCATTGACATGTACACAGAAGGAGTGGCGGATCTGGAGATAATGATTCTCTATTACCCCCACATGCCCCCTGAGGAGAAAGAGGCAAGCCTTGCCAAGATCAAGGAACAAACCAGGAACCGTTACTTCCCTGCCTTTGAAAAGGTGTTGAAGAGCCATGGACAAGATTATCTCGTTG
SRR25558762 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 14:57:29
                             Started mapping on |	May 24 14:57:29
                                    Finished on |	May 24 15:03:19
       Mapping speed, Million of reads per hour |	438.05

                          Number of input reads |	42587921
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	37064601
                        Uniquely mapped reads % |	87.03%
                          Average mapped length |	298.84
                       Number of splices: Total |	39849375
            Number of splices: Annotated (sjdb) |	39606984
                       Number of splices: GT/AG |	39569099
                       Number of splices: GC/AG |	237173
                       Number of splices: AT/AC |	16042
               Number of splices: Non-canonical |	27061
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.64
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2903406
             % of reads mapped to multiple loci |	6.82%
        Number of reads mapped to too many loci |	31832
             % of reads mapped to too many loci |	0.07%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.31%
                     % of reads unmapped: other |	4.76%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2619914	2619914	2619914
N_multimapping	2903406	2903406	2903406
N_noFeature	1515151	18590950	18639763
N_ambiguous	2401570	575766	566494
UnstrandedReadsAssigned:33147880 PositiveStrandReadsAssigned:17897885 NegativeStrandReadsAssigned:17858344
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25558762 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558762-trimmed-pair1.fastq
                             SRR25558762-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 42,587,921 reads, 39,969,145 reads pseudoaligned
[quant] estimated average fragment length: 236.964
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,065 rounds

 109283 SRR25558762.ke.tsv
  52636 SRR25558762.se.tsv
 161919 total
==> SRR25558762.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25558762.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558762 completed mapping pipeline successfully
