Starting /dee2/code/volunteer_pipeline.sh SRR25558757
    current disk space = 1169589872
    free memory = 190175240 
SRR25558757 SRAfilesize
6d011cac44462de804553aa71bf9226d  SRR25558757.sra
SRR25558757.sra file validated
SRR25558757 is paired end
SRR25558757 is conventional basespace
SRR25558757 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.53	37.0	37.0	37.0	37.0	37.0
2	36.38625	37.0	37.0	37.0	37.0	37.0
3	36.47525	37.0	37.0	37.0	37.0	37.0
4	36.606	37.0	37.0	37.0	37.0	37.0
5	36.4775	37.0	37.0	37.0	37.0	37.0
6	36.5315	37.0	37.0	37.0	37.0	37.0
7	36.5355	37.0	37.0	37.0	37.0	37.0
8	36.63	37.0	37.0	37.0	37.0	37.0
9	36.5515	37.0	37.0	37.0	37.0	37.0
10-14	36.5391	37.0	37.0	37.0	37.0	37.0
15-19	36.4813	37.0	37.0	37.0	37.0	37.0
20-24	36.4769	37.0	37.0	37.0	37.0	37.0
25-29	36.457300000000004	37.0	37.0	37.0	37.0	37.0
30-34	36.434799999999996	37.0	37.0	37.0	37.0	37.0
35-39	36.4339	37.0	37.0	37.0	37.0	37.0
40-44	36.4131	37.0	37.0	37.0	37.0	37.0
45-49	36.388099999999994	37.0	37.0	37.0	37.0	37.0
50-54	36.3899	37.0	37.0	37.0	37.0	37.0
55-59	36.3377	37.0	37.0	37.0	37.0	37.0
60-64	36.3379	37.0	37.0	37.0	37.0	37.0
65-69	36.3399	37.0	37.0	37.0	37.0	37.0
70-74	36.301700000000004	37.0	37.0	37.0	37.0	37.0
75-79	36.338300000000004	37.0	37.0	37.0	37.0	37.0
80-84	36.258500000000005	37.0	37.0	37.0	37.0	37.0
85-89	36.2775	37.0	37.0	37.0	37.0	37.0
90-94	36.2236	37.0	37.0	37.0	37.0	37.0
95-99	36.23350000000001	37.0	37.0	37.0	37.0	37.0
100-104	36.1619	37.0	37.0	37.0	37.0	37.0
105-109	36.1474	37.0	37.0	37.0	37.0	37.0
110-114	36.1468	37.0	37.0	37.0	37.0	37.0
115-119	36.12089999999999	37.0	37.0	37.0	37.0	37.0
120-124	36.0892	37.0	37.0	37.0	37.0	37.0
125-129	36.038500000000006	37.0	37.0	37.0	37.0	37.0
130-134	35.9647	37.0	37.0	37.0	37.0	37.0
135-139	35.9966	37.0	37.0	37.0	37.0	37.0
140-144	35.9057	37.0	37.0	37.0	37.0	37.0
145-149	35.927099999999996	37.0	37.0	37.0	37.0	37.0
150	35.853	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	1.0
24	1.0
25	1.0
26	3.0
27	7.0
28	10.0
29	26.0
30	24.0
31	37.0
32	54.0
33	69.0
34	106.0
35	290.0
36	2963.0
37	407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.05	15.275	17.4	41.275
2	22.70107742420446	25.833124530192936	33.425206715109	18.040591330493612
3	26.03150787696924	25.93148287071768	25.456364091022753	22.58064516129032
4	25.724999999999998	32.65	20.674999999999997	20.95
5	23.95	35.875	21.675	18.5
6	18.775	37.724999999999994	22.475	21.025
7	16.400000000000002	16.3	43.35	23.95
8	19.55	22.175	29.95	28.325
9	22.425	23.474999999999998	26.525	27.575
10-14	22.919999999999998	28.015	25.55	23.515
15-19	22.965	25.185000000000002	26.63	25.22
20-24	22.405	26.205000000000002	27.685	23.705000000000002
25-29	24.075	25.929999999999996	25.15	24.845
30-34	22.495	26.805	26.040000000000003	24.66
35-39	25.455	25.585	26.08	22.88
40-44	23.419999999999998	26.215	26.47	23.895
45-49	23.28	27.36	25.840000000000003	23.52
50-54	23.01	27.91	26.165	22.915
55-59	21.89	25.77	27.875	24.465
60-64	23.9	27.055	25.945	23.1
65-69	22.6	26.85	27.37	23.18
70-74	22.8	26.805	27.55	22.845
75-79	25.230000000000004	26.695	25.855	22.220000000000002
80-84	23.189999999999998	27.99	27.005000000000003	21.815
85-89	22.994999999999997	27.060000000000002	27.565	22.38
90-94	24.175	27.639999999999997	25.7	22.485
95-99	22.005	27.96	27.68	22.355
100-104	21.29	28.144999999999996	26.965	23.599999999999998
105-109	23.265	26.77	26.795	23.169999999999998
110-114	23.169999999999998	27.47	27.21	22.15
115-119	22.31	27.750000000000004	28.275	21.665
120-124	23.275000000000002	26.855	27.810000000000002	22.06
125-129	22.38	27.0	27.785	22.835
130-134	22.189999999999998	27.150000000000002	28.125	22.535
135-139	23.415	25.169999999999998	28.58	22.835
140-144	22.645	27.595	26.735	23.025000000000002
145-149	22.79	28.215	25.95	23.044999999999998
150	21.55	30.25	25.8	22.400000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	0.5
18	0.5
19	0.5
20	1.0
21	2.5
22	1.5
23	1.0
24	3.5
25	5.0
26	6.0
27	5.0
28	6.0
29	8.5
30	8.5
31	9.0
32	7.5
33	7.5
34	15.0
35	21.0
36	30.0
37	51.5
38	81.5
39	143.0
40	210.5
41	222.0
42	223.0
43	229.5
44	238.0
45	225.0
46	249.5
47	298.0
48	293.5
49	207.5
50	132.0
51	157.0
52	160.5
53	147.5
54	116.5
55	76.5
56	53.5
57	34.0
58	24.0
59	14.0
60	13.0
61	11.5
62	9.5
63	17.0
64	24.5
65	31.5
66	25.5
67	24.0
68	20.0
69	11.0
70	21.5
71	23.0
72	15.5
73	11.0
74	4.5
75	1.5
76	1.0
77	2.0
78	1.0
79	0.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.22499999999999998
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.525000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.268811028144746	22.75
2	21.71165996553705	18.9
3	10.511200459506032	13.725000000000001
4	5.399195864445721	9.4
5	3.2739804709936813	7.124999999999999
6	1.9529006318207927	5.1
7	1.1487650775416427	3.5000000000000004
8	0.8615738081562321	3.0
9	0.5169442848937392	2.025
>10	2.3549684089603677	14.475
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	33	0.8250000000000001	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	28	0.7000000000000001	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	27	0.675	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	23	0.575	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	19	0.475	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	17	0.42500000000000004	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	17	0.42500000000000004	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	16	0.4	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	15	0.375	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	15	0.375	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	15	0.375	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	14	0.35000000000000003	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	14	0.35000000000000003	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	14	0.35000000000000003	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	13	0.325	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	13	0.325	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	13	0.325	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	13	0.325	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	13	0.325	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	13	0.325	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	13	0.325	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	12	0.3	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	12	0.3	No Hit
CAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGG	12	0.3	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	12	0.3	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	12	0.3	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	12	0.3	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	12	0.3	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	11	0.27499999999999997	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	11	0.27499999999999997	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	11	0.27499999999999997	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	11	0.27499999999999997	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	11	0.27499999999999997	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	11	0.27499999999999997	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	11	0.27499999999999997	No Hit
CAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGAGC	10	0.25	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	10	0.25	No Hit
CCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA	10	0.25	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	10	0.25	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	10	0.25	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	10	0.25	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	9	0.22499999999999998	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	9	0.22499999999999998	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	9	0.22499999999999998	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	9	0.22499999999999998	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	9	0.22499999999999998	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	9	0.22499999999999998	No Hit
CTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCC	9	0.22499999999999998	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	9	0.22499999999999998	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	9	0.22499999999999998	No Hit
TTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTT	8	0.2	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	8	0.2	No Hit
GTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAG	8	0.2	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	8	0.2	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	8	0.2	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	8	0.2	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	8	0.2	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	8	0.2	No Hit
AAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGG	8	0.2	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	8	0.2	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	8	0.2	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	8	0.2	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	8	0.2	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	8	0.2	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	8	0.2	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	7	0.17500000000000002	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	7	0.17500000000000002	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	7	0.17500000000000002	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	7	0.17500000000000002	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	7	0.17500000000000002	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	7	0.17500000000000002	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	7	0.17500000000000002	No Hit
GGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGG	7	0.17500000000000002	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	7	0.17500000000000002	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	7	0.17500000000000002	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	7	0.17500000000000002	No Hit
TGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAAT	7	0.17500000000000002	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	7	0.17500000000000002	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	7	0.17500000000000002	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	7	0.17500000000000002	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	7	0.17500000000000002	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	7	0.17500000000000002	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	7	0.17500000000000002	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	6	0.15	No Hit
GCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAAT	6	0.15	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	6	0.15	No Hit
CTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCC	6	0.15	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	6	0.15	No Hit
TCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGA	6	0.15	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	6	0.15	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	6	0.15	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	6	0.15	No Hit
CAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCAT	6	0.15	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	6	0.15	No Hit
ACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGC	6	0.15	No Hit
GAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGC	6	0.15	No Hit
TGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAA	6	0.15	No Hit
AAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAA	6	0.15	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	6	0.15	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	6	0.15	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	6	0.15	No Hit
CTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCC	6	0.15	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	6	0.15	No Hit
CTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAA	6	0.15	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	6	0.15	No Hit
CCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAATGTTT	6	0.15	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	6	0.15	No Hit
TTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAA	6	0.15	No Hit
GTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCG	6	0.15	No Hit
CTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCA	6	0.15	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	6	0.15	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	6	0.15	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	6	0.15	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	6	0.15	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	6	0.15	No Hit
CATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATA	6	0.15	No Hit
CTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGT	6	0.15	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	5	0.125	No Hit
TAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCC	5	0.125	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	5	0.125	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	5	0.125	No Hit
CTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTCGATTA	5	0.125	No Hit
TGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTA	5	0.125	No Hit
CTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCAT	5	0.125	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	5	0.125	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	5	0.125	No Hit
AGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACA	5	0.125	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	5	0.125	No Hit
CTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCG	5	0.125	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	5	0.125	No Hit
GCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCC	5	0.125	No Hit
TCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCAC	5	0.125	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	5	0.125	No Hit
GCGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGG	5	0.125	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	5	0.125	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	5	0.125	No Hit
CAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCC	5	0.125	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	5	0.125	No Hit
CCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAG	5	0.125	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	5	0.125	No Hit
TGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCAT	5	0.125	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	5	0.125	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	5	0.125	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	5	0.125	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	5	0.125	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	5	0.125	No Hit
GAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCT	5	0.125	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	5	0.125	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	5	0.125	No Hit
CTGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATAGCCA	5	0.125	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	5	0.125	No Hit
GTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGC	5	0.125	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	5	0.125	No Hit
CGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGG	5	0.125	No Hit
AGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGG	5	0.125	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	5	0.125	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	5	0.125	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	5	0.125	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	5	0.125	No Hit
CCGATGCAAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGA	5	0.125	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	5	0.125	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	5	0.125	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	5	0.125	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	5	0.125	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	5	0.125	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	5	0.125	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	5	0.125	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	5	0.125	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	5	0.125	No Hit
GTTGAGTTGGATCAACTCGACCTAAATCAATGAGATACTGTAGTCTCCTG	5	0.125	No Hit
TTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGT	5	0.125	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	5	0.125	No Hit
GCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGCTG	5	0.125	No Hit
GTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25558757 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558757_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4895	37.0	37.0	37.0	37.0	37.0
2	36.3355	37.0	37.0	37.0	37.0	37.0
3	36.5445	37.0	37.0	37.0	37.0	37.0
4	36.3315	37.0	37.0	37.0	37.0	37.0
5	36.353	37.0	37.0	37.0	37.0	37.0
6	36.3515	37.0	37.0	37.0	37.0	37.0
7	36.384	37.0	37.0	37.0	37.0	37.0
8	36.4505	37.0	37.0	37.0	37.0	37.0
9	36.435	37.0	37.0	37.0	37.0	37.0
10-14	36.430099999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.4644	37.0	37.0	37.0	37.0	37.0
20-24	36.386	37.0	37.0	37.0	37.0	37.0
25-29	36.27120000000001	37.0	37.0	37.0	37.0	37.0
30-34	36.3615	37.0	37.0	37.0	37.0	37.0
35-39	36.310500000000005	37.0	37.0	37.0	37.0	37.0
40-44	36.2979	37.0	37.0	37.0	37.0	37.0
45-49	36.2912	37.0	37.0	37.0	37.0	37.0
50-54	36.2617	37.0	37.0	37.0	37.0	37.0
55-59	36.228	37.0	37.0	37.0	37.0	37.0
60-64	36.2109	37.0	37.0	37.0	37.0	37.0
65-69	36.1287	37.0	37.0	37.0	37.0	37.0
70-74	36.069	37.0	37.0	37.0	37.0	37.0
75-79	36.0351	37.0	37.0	37.0	37.0	37.0
80-84	36.0005	37.0	37.0	37.0	37.0	37.0
85-89	36.036500000000004	37.0	37.0	37.0	37.0	37.0
90-94	36.0298	37.0	37.0	37.0	37.0	37.0
95-99	35.90429999999999	37.0	37.0	37.0	37.0	37.0
100-104	35.8994	37.0	37.0	37.0	37.0	37.0
105-109	35.903499999999994	37.0	37.0	37.0	37.0	37.0
110-114	35.8482	37.0	37.0	37.0	37.0	37.0
115-119	35.696600000000004	37.0	37.0	37.0	37.0	37.0
120-124	35.644	37.0	37.0	37.0	37.0	37.0
125-129	35.5822	37.0	37.0	37.0	37.0	37.0
130-134	35.5955	37.0	37.0	37.0	37.0	37.0
135-139	35.5238	37.0	37.0	37.0	37.0	37.0
140-144	35.4773	37.0	37.0	37.0	37.0	37.0
145-149	35.3024	37.0	37.0	37.0	29.8	37.0
150	35.3155	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	1.0
22	0.0
23	0.0
24	4.0
25	1.0
26	4.0
27	14.0
28	10.0
29	21.0
30	22.0
31	33.0
32	54.0
33	84.0
34	180.0
35	594.0
36	2793.0
37	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.4	15.75	16.2	39.65
2	24.125	24.45	34.175	17.25
3	26.35	26.75	24.3	22.6
4	25.525	36.575	19.35	18.55
5	26.674999999999997	37.075	18.375	17.875
6	20.474999999999998	37.625	22.85	19.05
7	18.2	15.425	42.175000000000004	24.2
8	19.400000000000002	22.400000000000002	29.95	28.249999999999996
9	22.8	22.900000000000002	27.6	26.700000000000003
10-14	23.155	27.950000000000003	24.785	24.11
15-19	22.634999999999998	25.785000000000004	27.22	24.36
20-24	22.475	26.875	26.77	23.880000000000003
25-29	23.815	26.515	25.135	24.535
30-34	22.64	27.224999999999998	26.240000000000002	23.895
35-39	24.240000000000002	26.87	26.424999999999997	22.465
40-44	24.044999999999998	25.979999999999997	26.169999999999998	23.805
45-49	23.985	27.589999999999996	24.495	23.93
50-54	24.4	27.73	25.924999999999997	21.945
55-59	21.935	26.775	27.005000000000003	24.285
60-64	23.885	26.96	26.07	23.085
65-69	22.52	26.755000000000003	27.6	23.125
70-74	23.125	25.509999999999998	28.810000000000002	22.555
75-79	24.18	26.82	26.355	22.645
80-84	22.875	26.845000000000002	28.57	21.709999999999997
85-89	23.7	26.474999999999998	28.08	21.745
90-94	23.369999999999997	27.61	26.305	22.715
95-99	22.215	27.925	27.735	22.125
100-104	21.59	28.955	26.200000000000003	23.255
105-109	24.005000000000003	27.145000000000003	25.740000000000002	23.11
110-114	22.42	27.589999999999996	27.125	22.865
115-119	22.759999999999998	27.38	27.055	22.805
120-124	22.485	26.72	28.58	22.215
125-129	22.305	27.865000000000002	27.395000000000003	22.435
130-134	22.31	27.860000000000003	27.36	22.470000000000002
135-139	22.82	25.165	28.799999999999997	23.215
140-144	22.675	28.325	25.8	23.200000000000003
145-149	22.48	29.065	25.380000000000003	23.075000000000003
150	22.825	29.375	27.0	20.8
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.5
19	0.5
20	0.5
21	0.5
22	1.5
23	5.0
24	6.0
25	4.0
26	3.5
27	6.5
28	10.5
29	9.0
30	9.5
31	10.5
32	9.0
33	10.5
34	10.5
35	12.0
36	27.0
37	54.5
38	71.0
39	156.5
40	227.5
41	223.0
42	237.5
43	230.5
44	235.5
45	215.5
46	264.0
47	322.0
48	283.0
49	203.0
50	121.0
51	101.0
52	140.0
53	174.5
54	129.0
55	82.0
56	61.5
57	41.5
58	19.5
59	11.5
60	11.0
61	8.5
62	8.0
63	9.5
64	21.5
65	34.5
66	32.5
67	23.0
68	19.0
69	17.0
70	18.5
71	17.0
72	12.5
73	11.0
74	4.5
75	3.0
76	4.0
77	2.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.00576368876081	23.425
2	20.230547550432277	17.549999999999997
3	9.855907780979827	12.825000000000001
4	5.244956772334294	9.1
5	2.5936599423631126	5.625
6	2.2478386167146973	5.8500000000000005
7	1.8443804034582134	5.6000000000000005
8	1.037463976945245	3.5999999999999996
9	0.4034582132564841	1.575
>10	2.5360230547550433	14.85
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	43	1.075	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	25	0.625	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	24	0.6	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	23	0.575	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	19	0.475	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	16	0.4	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	16	0.4	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	15	0.375	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	14	0.35000000000000003	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	14	0.35000000000000003	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	13	0.325	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	13	0.325	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	13	0.325	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	13	0.325	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	13	0.325	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	12	0.3	No Hit
CGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGG	12	0.3	No Hit
TGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTA	12	0.3	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	12	0.3	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	12	0.3	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	12	0.3	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	12	0.3	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	12	0.3	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	12	0.3	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	12	0.3	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	11	0.27499999999999997	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	11	0.27499999999999997	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	11	0.27499999999999997	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	11	0.27499999999999997	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	11	0.27499999999999997	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	11	0.27499999999999997	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	11	0.27499999999999997	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	11	0.27499999999999997	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	11	0.27499999999999997	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	11	0.27499999999999997	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	10	0.25	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	10	0.25	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	10	0.25	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	10	0.25	No Hit
TGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGG	10	0.25	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	10	0.25	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	10	0.25	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	10	0.25	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	10	0.25	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	9	0.22499999999999998	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	9	0.22499999999999998	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	9	0.22499999999999998	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	9	0.22499999999999998	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	9	0.22499999999999998	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	9	0.22499999999999998	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	9	0.22499999999999998	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	8	0.2	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	8	0.2	No Hit
CGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCAT	8	0.2	No Hit
ATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTA	8	0.2	No Hit
TGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGC	8	0.2	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	8	0.2	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	8	0.2	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	8	0.2	No Hit
GCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCC	8	0.2	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	8	0.2	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	8	0.2	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	8	0.2	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	8	0.2	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	8	0.2	No Hit
GTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCG	8	0.2	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	8	0.2	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	8	0.2	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	8	0.2	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	7	0.17500000000000002	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	7	0.17500000000000002	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	7	0.17500000000000002	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	7	0.17500000000000002	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	7	0.17500000000000002	No Hit
ATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAG	7	0.17500000000000002	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	7	0.17500000000000002	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	7	0.17500000000000002	No Hit
TTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGT	7	0.17500000000000002	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	7	0.17500000000000002	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	7	0.17500000000000002	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	7	0.17500000000000002	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	7	0.17500000000000002	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	7	0.17500000000000002	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	7	0.17500000000000002	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	7	0.17500000000000002	No Hit
AACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCA	7	0.17500000000000002	No Hit
CAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTG	7	0.17500000000000002	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	7	0.17500000000000002	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	7	0.17500000000000002	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	7	0.17500000000000002	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	7	0.17500000000000002	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	7	0.17500000000000002	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	7	0.17500000000000002	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	7	0.17500000000000002	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	7	0.17500000000000002	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	7	0.17500000000000002	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	7	0.17500000000000002	No Hit
CACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGA	7	0.17500000000000002	No Hit
GTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATAT	7	0.17500000000000002	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	7	0.17500000000000002	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	7	0.17500000000000002	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	6	0.15	No Hit
TATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGC	6	0.15	No Hit
GCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGGTCAGTCATGG	6	0.15	No Hit
GCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGT	6	0.15	No Hit
GCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATA	6	0.15	No Hit
GGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCA	6	0.15	No Hit
CTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTCGATTA	6	0.15	No Hit
GTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAG	6	0.15	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	6	0.15	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	6	0.15	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	6	0.15	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	6	0.15	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	6	0.15	No Hit
CCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGA	6	0.15	No Hit
CAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCAT	6	0.15	No Hit
CGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGA	6	0.15	No Hit
CTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTGG	6	0.15	No Hit
ATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGG	6	0.15	No Hit
CTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCG	6	0.15	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	6	0.15	No Hit
CAACGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACT	6	0.15	No Hit
TGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCG	6	0.15	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	6	0.15	No Hit
GTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAG	6	0.15	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	6	0.15	No Hit
AGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTG	6	0.15	No Hit
CCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCC	6	0.15	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	6	0.15	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	6	0.15	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	6	0.15	No Hit
CTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCGATCAA	6	0.15	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	6	0.15	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	6	0.15	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGC	6	0.15	No Hit
GTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCC	6	0.15	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	6	0.15	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	6	0.15	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	6	0.15	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	6	0.15	No Hit
CAGGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCA	5	0.125	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	5	0.125	No Hit
CGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACTGGC	5	0.125	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	5	0.125	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	5	0.125	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	5	0.125	No Hit
GTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGT	5	0.125	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	5	0.125	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	5	0.125	No Hit
GCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCA	5	0.125	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	5	0.125	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	5	0.125	No Hit
CCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	5	0.125	No Hit
ATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGGGAAC	5	0.125	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	5	0.125	No Hit
TATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTT	5	0.125	No Hit
GCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGA	5	0.125	No Hit
CAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGAGC	5	0.125	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	5	0.125	No Hit
GGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCC	5	0.125	No Hit
AGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGG	5	0.125	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	5	0.125	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	5	0.125	No Hit
ATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATAT	5	0.125	No Hit
CTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGG	5	0.125	No Hit
CCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA	5	0.125	No Hit
TGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAA	5	0.125	No Hit
AAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAA	5	0.125	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	5	0.125	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	5	0.125	No Hit
AAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATT	5	0.125	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	5	0.125	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	5	0.125	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	5	0.125	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	5	0.125	No Hit
TGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATAT	5	0.125	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	5	0.125	No Hit
TAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCC	5	0.125	No Hit
TGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCA	5	0.125	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	5	0.125	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	5	0.125	No Hit
AACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCT	5	0.125	No Hit
GCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	10	0.006973645	144.0	6
TGGTAAC	10	0.006973645	144.0	1
TAACAGA	10	0.006973645	144.0	4
AACAGAC	10	0.006973645	144.0	5
AGACCCA	10	0.006973645	144.0	8
GTAACAG	10	0.006973645	144.0	3
GGTAACA	10	0.006973645	144.0	2
CAAGAAT	30	0.0018473949	72.0	1
AGAATCC	30	0.0018473949	72.0	3
AAGAATC	30	0.0018473949	72.0	2
GAATCCA	30	0.0018473949	72.0	4
CATGTGC	30	0.0018473949	72.0	9
ATCCATG	35	0.0034045284	61.714283	6
AATCCAT	45	0.009205684	48.0	5
CCATGTG	45	0.009205684	48.0	8
TCCATGT	45	0.009205684	48.0	7
>>END_MODULE
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367202 spots for SRR25558757.sra
Written 5367202 spots for SRR25558757.sra
Read 5367207 spots for SRR25558757.sra
Written 5367207 spots for SRR25558757.sra
SRR ids: ['SRR25558757.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_0v0o2h27
SRR25558757.sra spots: 42937621
blocks: [[1, 5367202], [5367203, 10734404], [10734405, 16101606], [16101607, 21468808], [21468809, 26836010], [26836011, 32203212], [32203213, 37570414], [37570415, 42937621]]
SRR25558757 file size 14486528
SRR25558757 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25558757 SRR25558757_1.fastq SRR25558757_2.fastq
Input file:	SRR25558757_1.fastq
Paired file:	SRR25558757_2.fastq
trimmed:	SRR25558757-trimmed-pair1.fastq, SRR25558757-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun May 24 15:24:36 2026 >> started

Sun May 24 15:25:57 2026 >> done (81.111s)
42937621 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       5 ( 0.00%) empty read pairs filtered out after trimming by size control
42937616 (100.00%) read pairs available; of these:
  416088 ( 0.97%) trimmed read pairs available after processing
42521528 (99.03%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 25	       1	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       1	  0.00%
 29	       0	  0.00%
 30	       2	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       1	  0.00%
 50	       1	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       1	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       2	  0.00%
 59	       1	  0.00%
 60	       0	  0.00%
 61	       1	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       1	  0.00%
 65	       0	  0.00%
 66	       1	  0.00%
 67	       1	  0.00%
 68	       4	  0.00%
 69	       4	  0.00%
 70	       4	  0.00%
 71	       6	  0.00%
 72	       4	  0.00%
 73	       5	  0.00%
 74	       3	  0.00%
 75	       8	  0.00%
 76	       4	  0.00%
 77	       3	  0.00%
 78	       6	  0.00%
 79	       3	  0.00%
 80	       7	  0.00%
 81	       8	  0.00%
 82	       7	  0.00%
 83	      11	  0.00%
 84	      10	  0.00%
 85	      15	  0.00%
 86	      16	  0.00%
 87	      10	  0.00%
 88	       8	  0.00%
 89	      13	  0.00%
 90	      23	  0.00%
 91	      20	  0.00%
 92	      19	  0.00%
 93	      25	  0.00%
 94	      21	  0.00%
 95	      36	  0.00%
 96	      33	  0.00%
 97	      50	  0.00%
 98	      35	  0.00%
 99	      41	  0.00%
100	      38	  0.00%
101	      49	  0.00%
102	      55	  0.00%
103	      67	  0.00%
104	      51	  0.00%
105	      79	  0.00%
106	      63	  0.00%
107	      78	  0.00%
108	      63	  0.00%
109	      77	  0.00%
110	      90	  0.00%
111	     107	  0.00%
112	     106	  0.00%
113	     132	  0.00%
114	     119	  0.00%
115	     134	  0.00%
116	     144	  0.00%
117	     148	  0.00%
118	     153	  0.00%
119	     184	  0.00%
120	     184	  0.00%
121	     227	  0.00%
122	     206	  0.00%
123	     238	  0.00%
124	     265	  0.00%
125	     274	  0.00%
126	     270	  0.00%
127	     313	  0.00%
128	     332	  0.00%
129	     309	  0.00%
130	     360	  0.00%
131	     354	  0.00%
132	     358	  0.00%
133	     425	  0.00%
134	     469	  0.00%
135	     490	  0.00%
136	     519	  0.00%
137	     535	  0.00%
138	     543	  0.00%
139	     589	  0.00%
140	     613	  0.00%
141	   36794	  0.09%
142	   38577	  0.09%
143	   40801	  0.10%
144	   42895	  0.10%
145	   44933	  0.10%
146	   47517	  0.11%
147	   49446	  0.12%
148	   51242	  0.12%
149	   53597	  0.12%
150	42521528	 99.03%
42937616 reads passed initial QC


criterion=sequence-density
sequence-density=2.19
sequence-density-rank=1
fanout-score=1.95
fanout-score-rank=33
prefix-density=2.21
prefix-fanout=1.9
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=178.16
fanout-score-rank=1
prefix-density=0.92
prefix-fanout=1.0
sequence=TGGTAAAAGCTAGCTTTTTAGAGGGAACAGCTTTTTAACAAGAAAACCCAAGCAACTGCTGCCATGGCGGGGAAGCCAGTCCTTCATTACTTTGATGGCAGGGGAAGAATGGAGCCTATCCGGTGGCTCTTGGCTGCAGCTGGTGTGGAGTTTGAAGAAAAATTTCTGAAAACTCGGGATGACCTGGCAAGGTTACGAAGTGATGGGAGTCTGATGTTCCAGCAAGTGCCCATGGTAGAGATCGACGGGATGAAACTGGTGCAGACCAAAGCCATTCTCAACTACATTGCCTCCAAATACAACCTCTATGGGAAGGACATGAAGGAGAGAGCCATCATTGACATGTACACAGAAGGAGTGGCGGATCTGGAGATAATGATTCTCTATTACCCCCACATGCCCCCTGAGGAGAAAGAGGCAAGCCTTGCCAAGATCAAGGAACAAACCAGGAACCGTTACTTCCCTGCCTTTGAAAAGGTGTTGAAGAGCCATGGACAAGATTATCTCGTTG


criterion=sequence-density
sequence-density=2.24
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=34
prefix-density=2.26
prefix-fanout=1.9
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=29
fanout-score=64.85
fanout-score-rank=1
prefix-density=1.93
prefix-fanout=1.0
sequence=CCTTCCTTTAATCTTAAGTAAAAGAGACACAGGGATTCAAAAATAAAAATTTCTTTCCCCATTCCCAGGCCTGTACCCAGTGCCCTCCATACCACCCTTCCCCTCTCTAACAGAAGCAAGGGAGGTTCAGCTTAACAGCCGCTGGGGGGGGTCAGAGGGGGGCTTCTGAGCTCAGTGTTGGTCTCTTTCCAAATATAAATACATGTGTCAAAACTGGGAACTCCTCCACACCCGTCACCCTGACGCCCTCCATTTCTGCTGGTGTTCGGGATGGGGAAGCCAGGCACCGACTGGCTGGGGTTTACTGCACACTTTGGGCATGGGCCCCACCAGTCTCCTGCTGCTCGTTGTAGAAGAGATGGCACTCGGGGTCTCCCCGGATGGTGGGAGCTCCCTGGATGGGCTTCCCGGTATTGGGGTTCACACACCAGCACTCCCCGCGCTGTCCGTTCAGAGACATCTTGCACTGCTTAAGGTTGTACCGGCCATGCTTGTCACAGTTGGGGATGTG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TTATTGGATCAACA -y TTATTGGATCAACA -o SRR25558757 SRR25558757_1.fastq SRR25558757_2.fastq
Input file:	SRR25558757_1.fastq
Paired file:	SRR25558757_2.fastq
trimmed:	SRR25558757-trimmed-pair1.fastq, SRR25558757-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TTATTGGATCAACA
-- paired 3' end adapter sequence (-y):	TTATTGGATCAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun May 24 15:28:07 2026 >> started

Sun May 24 15:28:18 2026 >> done (11.388s)
14312539 read pairs processed; of these:
      14 ( 0.00%) short read pairs filtered out after trimming by size control
      53 ( 0.00%) empty read pairs filtered out after trimming by size control
14312472 (100.00%) read pairs available; of these:
      66 ( 0.00%) trimmed read pairs available after processing
14312406 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 30	       2	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       1	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       1	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       1	  0.00%
 69	       1	  0.00%
 70	       2	  0.00%
 71	       2	  0.00%
 72	       1	  0.00%
 73	       1	  0.00%
 74	       1	  0.00%
 75	       1	  0.00%
 76	       0	  0.00%
 77	       0	  0.00%
 78	       4	  0.00%
 79	       1	  0.00%
 80	       2	  0.00%
 81	       3	  0.00%
 82	       2	  0.00%
 83	       5	  0.00%
 84	       2	  0.00%
 85	       7	  0.00%
 86	       6	  0.00%
 87	       2	  0.00%
 88	       3	  0.00%
 89	       2	  0.00%
 90	      11	  0.00%
 91	       7	  0.00%
 92	       6	  0.00%
 93	       6	  0.00%
 94	       7	  0.00%
 95	       6	  0.00%
 96	      12	  0.00%
 97	      10	  0.00%
 98	      14	  0.00%
 99	      13	  0.00%
100	      12	  0.00%
101	      14	  0.00%
102	      21	  0.00%
103	      23	  0.00%
104	      20	  0.00%
105	      28	  0.00%
106	      21	  0.00%
107	      19	  0.00%
108	      21	  0.00%
109	      28	  0.00%
110	      31	  0.00%
111	      34	  0.00%
112	      44	  0.00%
113	      42	  0.00%
114	      30	  0.00%
115	      40	  0.00%
116	      39	  0.00%
117	      47	  0.00%
118	      50	  0.00%
119	      53	  0.00%
120	      56	  0.00%
121	      85	  0.00%
122	      62	  0.00%
123	      86	  0.00%
124	      82	  0.00%
125	      80	  0.00%
126	      84	  0.00%
127	      92	  0.00%
128	     103	  0.00%
129	      99	  0.00%
130	     116	  0.00%
131	     121	  0.00%
132	     122	  0.00%
133	     127	  0.00%
134	     150	  0.00%
135	     154	  0.00%
136	     162	  0.00%
137	     147	  0.00%
138	     172	  0.00%
139	     219	  0.00%
140	     200	  0.00%
141	   12379	  0.09%
142	   12824	  0.09%
143	   14006	  0.10%
144	   14564	  0.10%
145	   15071	  0.11%
146	   15978	  0.11%
147	   16578	  0.12%
148	   17183	  0.12%
149	   18020	  0.13%
150	14172588	 99.02%


criterion=sequence-density
sequence-density=0.27
sequence-density-rank=1
fanout-score=3.97
fanout-score-rank=32
prefix-density=0.34
prefix-fanout=3.1
sequence=GAAGACATCCTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=71.13
fanout-score-rank=1
prefix-density=0.08
prefix-fanout=7.5
sequence=AAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGT


criterion=sequence-density
sequence-density=0.31
sequence-density-rank=1
fanout-score=3.74
fanout-score-rank=31
prefix-density=0.39
prefix-fanout=3.0
sequence=CGCCTGCAGGCGGAGATCTTCCAGGCCCGCCTCAAGGGCTGGTTCGAGCCAATAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGATGGAGAAGATACAGGCCTCTGTGGCTACCAACCCCATCATCACCCCAGTGGCCCAGGAGAATCAATGAGTATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTGGCCCTGTCTCAAGCACCTCTCTGGCCCTCTGGTGGCCCTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=60.75
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=7.0
sequence=AAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGT
SRR25558757 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 15:30:31
                             Started mapping on |	May 24 15:30:31
                                    Finished on |	May 24 15:37:36
       Mapping speed, Million of reads per hour |	363.71

                          Number of input reads |	42937549
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	39686670
                        Uniquely mapped reads % |	92.43%
                          Average mapped length |	298.96
                       Number of splices: Total |	43064688
            Number of splices: Annotated (sjdb) |	42810402
                       Number of splices: GT/AG |	42740066
                       Number of splices: GC/AG |	274560
                       Number of splices: AT/AC |	19641
               Number of splices: Non-canonical |	30421
                      Mismatch rate per base, % |	0.14%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.63
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.30
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2696852
             % of reads mapped to multiple loci |	6.28%
        Number of reads mapped to too many loci |	47033
             % of reads mapped to too many loci |	0.11%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.15%
                     % of reads unmapped: other |	0.04%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	554027	554027	554027
N_multimapping	2696852	2696852	2696852
N_noFeature	2029418	20095242	20193038
N_ambiguous	3146691	921617	908660
UnstrandedReadsAssigned:34510561 PositiveStrandReadsAssigned:18669811 NegativeStrandReadsAssigned:18584972
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25558757 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558757-trimmed-pair1.fastq
                             SRR25558757-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 42,937,549 reads, 39,809,252 reads pseudoaligned
[quant] estimated average fragment length: 235.666
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,068 rounds

 109283 SRR25558757.ke.tsv
  52636 SRR25558757.se.tsv
 161919 total
==> SRR25558757.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7.72727	0	0
ENSMUST00000196221.1	9	3.16667	0	0
ENSMUST00000179664.1	11	4.25	0	0
ENSMUST00000178537.1	12	4.77778	0	0
ENSMUST00000178862.1	14	6.3	0	0
ENSMUST00000179520.1	11	4.25	0	0
ENSMUST00000179883.1	16	7.72727	0	0
ENSMUST00000195858.1	10	3.71429	0	0
ENSMUST00000179932.1	12	4.77778	0	0

==> SRR25558757.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	3
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558757 completed mapping pipeline successfully
