Starting /dee2/code/volunteer_pipeline.sh SRR25558760
    current disk space = 460537126912
    free memory = 355436412 
SRR25558760 SRAfilesize
c4b65e0702dc64cf0f21adfdcc7fa037  SRR25558760.sra
SRR25558760.sra file validated
SRR25558760 is paired end
SRR25558760 is conventional basespace
SRR25558760 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.384	37.0	37.0	37.0	37.0	37.0
2	36.42425	37.0	37.0	37.0	37.0	37.0
3	36.449	37.0	37.0	37.0	37.0	37.0
4	36.358	37.0	37.0	37.0	37.0	37.0
5	36.4205	37.0	37.0	37.0	37.0	37.0
6	36.516	37.0	37.0	37.0	37.0	37.0
7	36.442	37.0	37.0	37.0	37.0	37.0
8	36.5045	37.0	37.0	37.0	37.0	37.0
9	36.438	37.0	37.0	37.0	37.0	37.0
10-14	36.488	37.0	37.0	37.0	37.0	37.0
15-19	36.4987	37.0	37.0	37.0	37.0	37.0
20-24	36.4863	37.0	37.0	37.0	37.0	37.0
25-29	36.4493	37.0	37.0	37.0	37.0	37.0
30-34	36.3661	37.0	37.0	37.0	37.0	37.0
35-39	36.3788	37.0	37.0	37.0	37.0	37.0
40-44	36.377700000000004	37.0	37.0	37.0	37.0	37.0
45-49	36.3312	37.0	37.0	37.0	37.0	37.0
50-54	36.307599999999994	37.0	37.0	37.0	37.0	37.0
55-59	36.328199999999995	37.0	37.0	37.0	37.0	37.0
60-64	36.2513	37.0	37.0	37.0	37.0	37.0
65-69	36.267999999999994	37.0	37.0	37.0	37.0	37.0
70-74	36.2611	37.0	37.0	37.0	37.0	37.0
75-79	36.2432	37.0	37.0	37.0	37.0	37.0
80-84	36.1978	37.0	37.0	37.0	37.0	37.0
85-89	36.2175	37.0	37.0	37.0	37.0	37.0
90-94	36.1327	37.0	37.0	37.0	37.0	37.0
95-99	36.1798	37.0	37.0	37.0	37.0	37.0
100-104	36.095800000000004	37.0	37.0	37.0	37.0	37.0
105-109	36.0311	37.0	37.0	37.0	37.0	37.0
110-114	36.12859999999999	37.0	37.0	37.0	37.0	37.0
115-119	36.1025	37.0	37.0	37.0	37.0	37.0
120-124	36.0568	37.0	37.0	37.0	37.0	37.0
125-129	35.9951	37.0	37.0	37.0	37.0	37.0
130-134	35.9216	37.0	37.0	37.0	37.0	37.0
135-139	36.0133	37.0	37.0	37.0	37.0	37.0
140-144	35.9587	37.0	37.0	37.0	37.0	37.0
145-149	35.8746	37.0	37.0	37.0	37.0	37.0
150	35.833	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	1.0
26	3.0
27	3.0
28	11.0
29	16.0
30	37.0
31	43.0
32	59.0
33	76.0
34	128.0
35	281.0
36	3072.0
37	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.989994997498748	16.08304152076038	17.958979489744873	35.967983991996
2	21.927409261576972	26.382978723404253	34.69336670838548	16.99624530663329
3	23.0	30.4	25.2	21.4
4	24.099999999999998	36.449999999999996	21.3	18.15
5	24.4	36.825	20.65	18.125
6	18.425	39.85	23.200000000000003	18.525
7	16.3	18.475	43.2	22.025
8	18.2	23.95	31.275	26.575
9	23.599999999999998	23.799999999999997	27.450000000000003	25.15
10-14	22.08	30.28	24.93	22.71
15-19	22.21	25.56	28.23	24.0
20-24	21.044999999999998	29.160000000000004	27.975	21.82
25-29	22.465	27.200000000000003	26.529999999999998	23.805
30-34	21.165	28.000000000000004	28.439999999999998	22.395
35-39	22.61	27.900000000000002	27.3	22.189999999999998
40-44	23.28	26.43	26.895000000000003	23.395
45-49	21.925	28.305000000000003	27.125	22.645
50-54	21.685	29.285	27.42	21.61
55-59	21.19	28.12	28.02	22.67
60-64	22.67	28.499999999999996	28.055000000000003	20.775
65-69	21.665	28.155	28.38	21.8
70-74	21.865000000000002	26.935	28.849999999999998	22.35
75-79	22.88	28.804999999999996	26.284999999999997	22.03
80-84	21.825	28.060000000000002	29.635	20.48
85-89	22.05	28.365000000000002	27.685	21.9
90-94	22.225	29.59	26.240000000000002	21.945
95-99	21.48	29.035	29.544999999999998	19.939999999999998
100-104	21.05	29.42	27.025	22.505
105-109	21.715	29.075	27.215	21.995
110-114	21.54	27.97	28.884999999999998	21.605
115-119	21.310000000000002	28.610000000000003	28.405	21.675
120-124	21.545	27.735	29.439999999999998	21.279999999999998
125-129	21.44	27.825	29.445	21.29
130-134	20.485	28.285	29.38	21.85
135-139	21.745	27.029999999999998	29.315	21.91
140-144	21.175	28.46	28.599999999999998	21.765
145-149	21.775	29.79	26.655	21.78
150	22.1	32.875	25.4	19.625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	0.5
21	1.0
22	0.5
23	1.5
24	3.0
25	1.5
26	7.0
27	28.5
28	80.5
29	100.0
30	75.0
31	48.5
32	35.0
33	43.0
34	43.5
35	48.5
36	87.0
37	105.0
38	78.5
39	125.0
40	193.5
41	216.0
42	213.0
43	196.5
44	212.5
45	180.0
46	225.0
47	269.0
48	221.5
49	167.5
50	114.5
51	118.0
52	135.0
53	148.0
54	112.5
55	63.5
56	46.5
57	37.0
58	18.5
59	10.0
60	10.5
61	6.5
62	4.0
63	6.5
64	15.0
65	25.5
66	24.0
67	18.5
68	17.0
69	13.0
70	10.5
71	9.0
72	9.5
73	8.0
74	3.0
75	1.5
76	0.0
77	0.0
78	1.5
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.536287732819524	17.724999999999998
2	22.28644829800899	17.349999999999998
3	11.689145793192036	13.65
4	7.578676942838793	11.799999999999999
5	4.046242774566474	7.875
6	2.569043031470777	6.0
7	1.991008349389852	5.425
8	0.8991650610147719	2.8000000000000003
9	0.8991650610147719	3.15
>10	2.5048169556840074	14.224999999999998
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	42	1.05	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	23	0.575	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	23	0.575	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	22	0.5499999999999999	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	21	0.525	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	20	0.5	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	20	0.5	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	20	0.5	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	18	0.44999999999999996	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	15	0.375	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	15	0.375	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	15	0.375	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	14	0.35000000000000003	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	14	0.35000000000000003	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	14	0.35000000000000003	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	14	0.35000000000000003	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	13	0.325	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	13	0.325	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	13	0.325	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	13	0.325	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	12	0.3	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	12	0.3	No Hit
GAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCT	12	0.3	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	12	0.3	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	12	0.3	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	11	0.27499999999999997	No Hit
CCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTC	11	0.27499999999999997	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	11	0.27499999999999997	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	11	0.27499999999999997	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	11	0.27499999999999997	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	11	0.27499999999999997	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	11	0.27499999999999997	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	10	0.25	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	10	0.25	No Hit
GTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTT	10	0.25	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	10	0.25	No Hit
GTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACT	10	0.25	No Hit
CTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTG	10	0.25	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	10	0.25	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	9	0.22499999999999998	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	9	0.22499999999999998	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	9	0.22499999999999998	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	9	0.22499999999999998	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	9	0.22499999999999998	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	9	0.22499999999999998	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	9	0.22499999999999998	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	9	0.22499999999999998	No Hit
AAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTC	9	0.22499999999999998	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	9	0.22499999999999998	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	9	0.22499999999999998	No Hit
CTCAAAAGCATTATCCCCATTATTAGGGATAATGTAATACCATCTGATTC	9	0.22499999999999998	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	9	0.22499999999999998	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	9	0.22499999999999998	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	8	0.2	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	8	0.2	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	8	0.2	No Hit
GATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACA	8	0.2	No Hit
CTTTTAAGTGTCTATGCATATATTTGTATTATACAATTCAGAATATACTA	8	0.2	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	8	0.2	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	8	0.2	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	8	0.2	No Hit
GAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCA	8	0.2	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	8	0.2	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	8	0.2	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	8	0.2	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	8	0.2	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	8	0.2	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	7	0.17500000000000002	No Hit
TAAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTA	7	0.17500000000000002	No Hit
GTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGACA	7	0.17500000000000002	No Hit
ATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAA	7	0.17500000000000002	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	7	0.17500000000000002	No Hit
TTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTT	7	0.17500000000000002	No Hit
CTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAG	7	0.17500000000000002	No Hit
GGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGC	7	0.17500000000000002	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	7	0.17500000000000002	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	7	0.17500000000000002	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	7	0.17500000000000002	No Hit
TTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACAC	7	0.17500000000000002	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	7	0.17500000000000002	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	7	0.17500000000000002	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	7	0.17500000000000002	No Hit
AAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGT	7	0.17500000000000002	No Hit
CAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGG	7	0.17500000000000002	No Hit
ATATGATCCTTTTAAGTGTCTATGCATATATTTGTATTATACAATTCAGA	7	0.17500000000000002	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	7	0.17500000000000002	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	7	0.17500000000000002	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	7	0.17500000000000002	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	7	0.17500000000000002	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	7	0.17500000000000002	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	7	0.17500000000000002	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	7	0.17500000000000002	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGC	7	0.17500000000000002	No Hit
CAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACT	7	0.17500000000000002	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	7	0.17500000000000002	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	7	0.17500000000000002	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	7	0.17500000000000002	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	7	0.17500000000000002	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	6	0.15	No Hit
TCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCAT	6	0.15	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	6	0.15	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	6	0.15	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	6	0.15	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	6	0.15	No Hit
AGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTC	6	0.15	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	6	0.15	No Hit
TGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAG	6	0.15	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	6	0.15	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	6	0.15	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	6	0.15	No Hit
TATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTT	6	0.15	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	6	0.15	No Hit
CAAAACTGGAACTACAGTCCCACACGATGGTACTCGAAACTGGAACTACA	6	0.15	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	6	0.15	No Hit
AAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGA	6	0.15	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	6	0.15	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	6	0.15	No Hit
CTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAA	6	0.15	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	6	0.15	No Hit
CGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCC	6	0.15	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	6	0.15	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	6	0.15	No Hit
TAATGTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCA	6	0.15	No Hit
CTAGTATGTTCTGTGAAGATGTGTTCAGGTAAGATGTAAGTGACTTCTTA	6	0.15	No Hit
GCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATT	6	0.15	No Hit
GAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACT	6	0.15	No Hit
CTAAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTAT	6	0.15	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	6	0.15	No Hit
CTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCT	6	0.15	No Hit
AGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAAT	6	0.15	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	6	0.15	No Hit
AGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAAA	6	0.15	No Hit
TGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAG	6	0.15	No Hit
GAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAG	6	0.15	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	6	0.15	No Hit
TGACACTCAAAAGCATTATCCCCATTATTAGGGATAATGTAATACCATCT	6	0.15	No Hit
CCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAG	6	0.15	No Hit
AAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGC	6	0.15	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	5	0.125	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	5	0.125	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	5	0.125	No Hit
AAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAA	5	0.125	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	5	0.125	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	5	0.125	No Hit
GTAACATCATTTAGTCTGTATCATTGGTATGTATTTGCAAAACATTTTAA	5	0.125	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	5	0.125	No Hit
TATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCC	5	0.125	No Hit
TAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCT	5	0.125	No Hit
TTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAATGATAC	5	0.125	No Hit
CTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTATTACATCA	5	0.125	No Hit
GTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGA	5	0.125	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	5	0.125	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	5	0.125	No Hit
AAAATGTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACCC	5	0.125	No Hit
ATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAG	5	0.125	No Hit
GCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTA	5	0.125	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	5	0.125	No Hit
CTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGA	5	0.125	No Hit
GCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAG	5	0.125	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	5	0.125	No Hit
GGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAG	5	0.125	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	5	0.125	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	5	0.125	No Hit
ATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGG	5	0.125	No Hit
CTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTT	5	0.125	No Hit
GTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGG	5	0.125	No Hit
AAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAA	5	0.125	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	5	0.125	No Hit
CTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTT	5	0.125	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	5	0.125	No Hit
GGATAATGTAATACCATCTGATTCTTAGCTATTATAGTATCATCTATGTG	5	0.125	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	5	0.125	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	5	0.125	No Hit
AAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAA	5	0.125	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	5	0.125	No Hit
ATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCC	5	0.125	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	5	0.125	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	5	0.125	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	5	0.125	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	5	0.125	No Hit
AATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACC	5	0.125	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	5	0.125	No Hit
GCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCA	5	0.125	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	5	0.125	No Hit
CTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGC	5	0.125	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	5	0.125	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	5	0.125	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	5	0.125	No Hit
TGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCT	5	0.125	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	5	0.125	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	5	0.125	No Hit
CCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTT	5	0.125	No Hit
TTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCCAGATGC	5	0.125	No Hit
AAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCA	5	0.125	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	5	0.125	No Hit
TTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAAT	5	0.125	No Hit
AGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAG	5	0.125	No Hit
CACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGAC	5	0.125	No Hit
CCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACT	5	0.125	No Hit
GTTAACTGCAAGGCACGCTATTAGGTTGAACCTAATTAGACAATTAATTC	5	0.125	No Hit
GGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0125	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	10	0.0069754543	143.9875	5
TAACAGA	10	0.0069754543	143.9875	3
CAGACCC	10	0.0069754543	143.9875	6
ACCCAAA	10	0.0069754543	143.9875	9
AACAGAC	10	0.0069754543	143.9875	4
AGACCCA	10	0.0069754543	143.9875	7
>>END_MODULE
SRR25558760 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558760_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.476	37.0	37.0	37.0	37.0	37.0
2	36.363	37.0	37.0	37.0	37.0	37.0
3	36.451	37.0	37.0	37.0	37.0	37.0
4	36.4225	37.0	37.0	37.0	37.0	37.0
5	36.403	37.0	37.0	37.0	37.0	37.0
6	36.337	37.0	37.0	37.0	37.0	37.0
7	36.398	37.0	37.0	37.0	37.0	37.0
8	36.3155	37.0	37.0	37.0	37.0	37.0
9	36.469	37.0	37.0	37.0	37.0	37.0
10-14	36.3729	37.0	37.0	37.0	37.0	37.0
15-19	36.3731	37.0	37.0	37.0	37.0	37.0
20-24	36.382799999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.30765	37.0	37.0	37.0	37.0	37.0
30-34	36.3404	37.0	37.0	37.0	37.0	37.0
35-39	36.3125	37.0	37.0	37.0	37.0	37.0
40-44	36.2761	37.0	37.0	37.0	37.0	37.0
45-49	36.2709	37.0	37.0	37.0	37.0	37.0
50-54	36.1289	37.0	37.0	37.0	37.0	37.0
55-59	36.197700000000005	37.0	37.0	37.0	37.0	37.0
60-64	36.1562	37.0	37.0	37.0	37.0	37.0
65-69	36.156949999999995	37.0	37.0	37.0	37.0	37.0
70-74	36.05025	37.0	37.0	37.0	37.0	37.0
75-79	36.0309	37.0	37.0	37.0	37.0	37.0
80-84	36.038599999999995	37.0	37.0	37.0	37.0	37.0
85-89	35.995400000000004	37.0	37.0	37.0	37.0	37.0
90-94	36.007999999999996	37.0	37.0	37.0	37.0	37.0
95-99	35.85549999999999	37.0	37.0	37.0	37.0	37.0
100-104	35.816900000000004	37.0	37.0	37.0	37.0	37.0
105-109	35.9168	37.0	37.0	37.0	37.0	37.0
110-114	35.835	37.0	37.0	37.0	37.0	37.0
115-119	35.7143	37.0	37.0	37.0	37.0	37.0
120-124	35.630100000000006	37.0	37.0	37.0	37.0	37.0
125-129	35.6374	37.0	37.0	37.0	37.0	37.0
130-134	35.5988	37.0	37.0	37.0	37.0	37.0
135-139	35.568599999999996	37.0	37.0	37.0	37.0	37.0
140-144	35.6332	37.0	37.0	37.0	37.0	37.0
145-149	35.4086	37.0	37.0	37.0	32.2	37.0
150	35.0625	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	4.0
25	6.0
26	8.0
27	16.0
28	15.0
29	18.0
30	27.0
31	27.0
32	63.0
33	83.0
34	178.0
35	493.0
36	2877.0
37	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.3	15.950000000000001	18.525	38.224999999999994
2	23.025000000000002	25.95	35.0	16.025
3	25.275	29.2	25.074999999999996	20.45
4	24.25	35.925000000000004	20.549999999999997	19.275000000000002
5	24.325	37.05	21.25	17.375
6	20.150000000000002	38.65	23.474999999999998	17.724999999999998
7	16.85	19.675	42.125	21.349999999999998
8	18.3	24.675	31.1	25.924999999999997
9	23.1	22.475	28.449999999999996	25.974999999999998
10-14	21.9	30.275000000000002	26.105	21.72
15-19	22.264999999999997	26.38	28.294999999999998	23.06
20-24	21.34	28.675	28.285	21.7
25-29	22.25111255562778	28.061403070153506	26.131306565328266	23.556177808890443
30-34	21.0	28.305000000000003	27.235	23.46
35-39	22.285	27.98	27.315	22.42
40-44	22.485	26.490000000000002	27.88	23.145
45-49	21.73	28.99	26.6	22.68
50-54	21.279999999999998	28.610000000000003	27.994999999999997	22.115000000000002
55-59	20.925	26.919999999999998	30.04	22.115000000000002
60-64	23.169999999999998	28.499999999999996	27.644999999999996	20.685000000000002
65-69	22.33111655582779	27.716385819290963	28.32641632081604	21.626081304065202
70-74	21.75608780439022	27.906395319765988	28.351417570878546	21.986099304965247
75-79	22.695	28.325	27.060000000000002	21.92
80-84	22.314999999999998	28.32	28.775000000000002	20.59
85-89	21.565	29.060000000000002	27.805000000000003	21.57
90-94	22.919999999999998	29.035	26.474999999999998	21.57
95-99	21.035	29.544999999999998	29.599999999999998	19.82
100-104	20.29	28.585	28.315	22.81
105-109	21.355	28.939999999999998	27.515	22.189999999999998
110-114	21.63	27.915	29.080000000000002	21.375
115-119	21.575	29.255	27.810000000000002	21.36
120-124	21.565	28.13	29.62	20.685000000000002
125-129	21.48	28.075	29.415000000000003	21.029999999999998
130-134	20.66	27.62	29.645	22.075
135-139	21.65	26.945000000000004	29.465000000000003	21.94
140-144	20.89	28.17	28.375	22.564999999999998
145-149	21.545	28.735	26.91	22.81
150	20.974999999999998	30.725	28.15	20.150000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	0.5
11	0.0
12	0.5
13	1.0
14	1.0
15	0.5
16	0.5
17	0.5
18	0.5
19	0.5
20	0.5
21	1.0
22	2.0
23	3.5
24	5.5
25	3.5
26	7.5
27	30.0
28	80.5
29	103.0
30	76.0
31	44.5
32	29.5
33	36.5
34	64.0
35	71.5
36	68.0
37	82.5
38	86.5
39	128.5
40	180.5
41	211.0
42	228.0
43	220.5
44	229.5
45	188.0
46	238.0
47	274.5
48	205.0
49	156.0
50	117.0
51	108.0
52	112.0
53	126.0
54	105.0
55	73.5
56	43.0
57	29.0
58	19.0
59	6.5
60	5.0
61	8.0
62	10.0
63	8.5
64	9.0
65	25.0
66	26.5
67	18.0
68	18.5
69	13.5
70	13.0
71	19.0
72	15.0
73	5.5
74	2.0
75	1.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.005
70-74	0.005
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.000618429189856	19.0
2	22.325293753865182	18.05
3	11.317254174397032	13.725000000000001
4	7.2974644403215825	11.799999999999999
5	4.1434755720470005	8.375
6	2.350030921459493	5.7
7	1.9789734075448362	5.6000000000000005
8	0.927643784786642	3.0
9	0.49474335188620905	1.7999999999999998
>10	2.1645021645021645	12.950000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	44	1.0999999999999999	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	27	0.675	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	24	0.6	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	20	0.5	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	19	0.475	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	18	0.44999999999999996	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	17	0.42500000000000004	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	16	0.4	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	15	0.375	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	15	0.375	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	15	0.375	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	14	0.35000000000000003	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	14	0.35000000000000003	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	14	0.35000000000000003	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	13	0.325	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	13	0.325	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	13	0.325	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	13	0.325	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	13	0.325	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	13	0.325	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	13	0.325	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	13	0.325	No Hit
CTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGT	12	0.3	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	12	0.3	No Hit
GGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAG	12	0.3	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	12	0.3	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	11	0.27499999999999997	No Hit
CTTTTAAGTGTCTATGCATATATTTGTATTATACAATTCAGAATATACTA	11	0.27499999999999997	No Hit
GTATAAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTG	11	0.27499999999999997	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	11	0.27499999999999997	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	10	0.25	No Hit
CTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCG	10	0.25	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	10	0.25	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	10	0.25	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	10	0.25	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	9	0.22499999999999998	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	9	0.22499999999999998	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	9	0.22499999999999998	No Hit
CAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCT	9	0.22499999999999998	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	9	0.22499999999999998	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	9	0.22499999999999998	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	9	0.22499999999999998	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	9	0.22499999999999998	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	8	0.2	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	8	0.2	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	8	0.2	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	8	0.2	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	8	0.2	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	8	0.2	No Hit
CTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTG	8	0.2	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	8	0.2	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	8	0.2	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	8	0.2	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	8	0.2	No Hit
CCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCT	8	0.2	No Hit
AGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAAT	8	0.2	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	8	0.2	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	8	0.2	No Hit
GTAACATCATTTAGTCTGTATCATTGGTATGTATTTGCAAAACATTTTAA	7	0.17500000000000002	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	7	0.17500000000000002	No Hit
TGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACA	7	0.17500000000000002	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	7	0.17500000000000002	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	7	0.17500000000000002	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	7	0.17500000000000002	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	7	0.17500000000000002	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	7	0.17500000000000002	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	7	0.17500000000000002	No Hit
ATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAG	7	0.17500000000000002	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	7	0.17500000000000002	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	7	0.17500000000000002	No Hit
CTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATA	7	0.17500000000000002	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	7	0.17500000000000002	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	7	0.17500000000000002	No Hit
CTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAA	7	0.17500000000000002	No Hit
GTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGG	7	0.17500000000000002	No Hit
GGGAAATATATGATCCTTTTAAGTGTCTATGCATATATTTGTATTATACA	7	0.17500000000000002	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	7	0.17500000000000002	No Hit
AAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGT	7	0.17500000000000002	No Hit
GGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGG	7	0.17500000000000002	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	7	0.17500000000000002	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	7	0.17500000000000002	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	7	0.17500000000000002	No Hit
CTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGC	7	0.17500000000000002	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	7	0.17500000000000002	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	7	0.17500000000000002	No Hit
TGAAGATGTGTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTA	7	0.17500000000000002	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	7	0.17500000000000002	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	7	0.17500000000000002	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	7	0.17500000000000002	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	7	0.17500000000000002	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	6	0.15	No Hit
CAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTT	6	0.15	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	6	0.15	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	6	0.15	No Hit
GGCGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGG	6	0.15	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	6	0.15	No Hit
CTGTGATGCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTT	6	0.15	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	6	0.15	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	6	0.15	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	6	0.15	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	6	0.15	No Hit
TCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTT	6	0.15	No Hit
CTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAG	6	0.15	No Hit
GTGCCCTATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGACCCTG	6	0.15	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	6	0.15	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	6	0.15	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	6	0.15	No Hit
TGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTG	6	0.15	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	6	0.15	No Hit
AGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTC	6	0.15	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	6	0.15	No Hit
TTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTTCAGGTAAGATGTAA	6	0.15	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	6	0.15	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	6	0.15	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCA	6	0.15	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	6	0.15	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	6	0.15	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	6	0.15	No Hit
CGCATAGACACTTAAAAGGATCATATATTTCCCATGAGGGTAACATCATT	6	0.15	No Hit
CTATGGTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGT	6	0.15	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	6	0.15	No Hit
CTCAAAAGCATTATCCCCATTATTAGGGATAATGTAATACCATCTGATTC	6	0.15	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	6	0.15	No Hit
TGACACTCAAAAGCATTATCCCCATTATTAGGGATAATGTAATACCATCT	6	0.15	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	6	0.15	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	6	0.15	No Hit
CTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTAT	6	0.15	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	6	0.15	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	5	0.125	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	5	0.125	No Hit
CACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTAT	5	0.125	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	5	0.125	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	5	0.125	No Hit
TCCCTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGG	5	0.125	No Hit
GGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTT	5	0.125	No Hit
CTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCC	5	0.125	No Hit
AAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCAT	5	0.125	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	5	0.125	No Hit
GTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGACA	5	0.125	No Hit
CTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTATTACATCA	5	0.125	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	5	0.125	No Hit
CGTATGTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGT	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
GATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACA	5	0.125	No Hit
CTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAA	5	0.125	No Hit
ATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAG	5	0.125	No Hit
CTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTG	5	0.125	No Hit
TTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGA	5	0.125	No Hit
TTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGA	5	0.125	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	5	0.125	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	5	0.125	No Hit
CCTTATCTCAACAAACTTTTTAAGTTAAGTGCTTTGAAATTCTTTTTTTT	5	0.125	No Hit
GAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAGG	5	0.125	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	5	0.125	No Hit
AAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGA	5	0.125	No Hit
ATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTT	5	0.125	No Hit
TAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTATT	5	0.125	No Hit
GGACTATAGTTCCAGTTTTGAGTACCATCGTGTGGGACTGTAGTTACAGT	5	0.125	No Hit
AGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGCC	5	0.125	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	5	0.125	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	5	0.125	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	5	0.125	No Hit
TAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCA	5	0.125	No Hit
GTCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGG	5	0.125	No Hit
TATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAAT	5	0.125	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	5	0.125	No Hit
GCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGT	5	0.125	No Hit
TGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCG	5	0.125	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	5	0.125	No Hit
AGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCAC	5	0.125	No Hit
GTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAA	5	0.125	No Hit
TGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTT	5	0.125	No Hit
TTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAA	5	0.125	No Hit
TGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAAT	5	0.125	No Hit
TGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAA	5	0.125	No Hit
GTTTGTTTGAACTTTATTTTTATTTTGTTTTTTAGTCTATGTGTATGTGT	5	0.125	No Hit
TTTACTTCGGGGAACTGCATCAACTTTTGTGAGATCATGCTTTATCCTCT	5	0.125	No Hit
CTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAA	5	0.125	No Hit
GCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTA	5	0.125	No Hit
GTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCG	5	0.125	No Hit
AAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTT	5	0.125	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	5	0.125	No Hit
CGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACT	5	0.125	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	5	0.125	No Hit
CTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGG	5	0.125	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	5	0.125	No Hit
CTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCA	5	0.125	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	5	0.125	No Hit
TAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCC	5	0.125	No Hit
ATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCAC	5	0.125	No Hit
CCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCA	5	0.125	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	5	0.125	No Hit
AGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGA	5	0.125	No Hit
GCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	5	0.125	No Hit
TGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0125	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
Read 2465495 spots for SRR25558760.sra
Written 2465495 spots for SRR25558760.sra
SRR ids: ['SRR25558760.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_luqk7hvv
SRR25558760.sra spots: 39447920
blocks: [[1, 2465495], [2465496, 4930990], [4930991, 7396485], [7396486, 9861980], [9861981, 12327475], [12327476, 14792970], [14792971, 17258465], [17258466, 19723960], [19723961, 22189455], [22189456, 24654950], [24654951, 27120445], [27120446, 29585940], [29585941, 32051435], [32051436, 34516930], [34516931, 36982425], [36982426, 39447920]]
SRR25558760 file size 1646879
SRR25558760 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25558760 SRR25558760_1.fastq SRR25558760_2.fastq
Input file:	SRR25558760_1.fastq
Paired file:	SRR25558760_2.fastq
trimmed:	SRR25558760-trimmed-pair1.fastq, SRR25558760-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 15:37:38 2026 >> started

Sun May 24 15:39:43 2026 >> done (124.638s)
39447920 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
      17 ( 0.00%) empty read pairs filtered out after trimming by size control
39447903 (100.00%) read pairs available; of these:
  266116 ( 0.67%) trimmed read pairs available after processing
39181787 (99.33%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 60	       1	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       1	  0.00%
 65	       2	  0.00%
 66	       1	  0.00%
 67	       3	  0.00%
 68	       3	  0.00%
 69	       1	  0.00%
 70	       4	  0.00%
 71	       5	  0.00%
 72	       2	  0.00%
 73	       4	  0.00%
 74	       2	  0.00%
 75	       1	  0.00%
 76	       4	  0.00%
 77	       2	  0.00%
 78	       3	  0.00%
 79	       7	  0.00%
 80	       3	  0.00%
 81	       5	  0.00%
 82	       7	  0.00%
 83	       7	  0.00%
 84	       3	  0.00%
 85	       7	  0.00%
 86	       6	  0.00%
 87	       2	  0.00%
 88	       6	  0.00%
 89	       4	  0.00%
 90	       9	  0.00%
 91	       9	  0.00%
 92	      10	  0.00%
 93	      11	  0.00%
 94	      18	  0.00%
 95	      14	  0.00%
 96	      21	  0.00%
 97	      13	  0.00%
 98	      22	  0.00%
 99	      17	  0.00%
100	      30	  0.00%
101	      22	  0.00%
102	      36	  0.00%
103	      37	  0.00%
104	      38	  0.00%
105	      39	  0.00%
106	      31	  0.00%
107	      52	  0.00%
108	      50	  0.00%
109	      52	  0.00%
110	      46	  0.00%
111	      67	  0.00%
112	      72	  0.00%
113	      67	  0.00%
114	      99	  0.00%
115	      59	  0.00%
116	      74	  0.00%
117	      86	  0.00%
118	     103	  0.00%
119	      98	  0.00%
120	     130	  0.00%
121	     128	  0.00%
122	     116	  0.00%
123	     143	  0.00%
124	     148	  0.00%
125	     179	  0.00%
126	     139	  0.00%
127	     192	  0.00%
128	     194	  0.00%
129	     213	  0.00%
130	     199	  0.00%
131	     246	  0.00%
132	     265	  0.00%
133	     271	  0.00%
134	     286	  0.00%
135	     325	  0.00%
136	     339	  0.00%
137	     365	  0.00%
138	     373	  0.00%
139	     386	  0.00%
140	     402	  0.00%
141	   22997	  0.06%
142	   24359	  0.06%
143	   25726	  0.07%
144	   27420	  0.07%
145	   28464	  0.07%
146	   30802	  0.08%
147	   31927	  0.08%
148	   33239	  0.08%
149	   34745	  0.09%
150	39181787	 99.33%
39447903 reads passed initial QC


criterion=sequence-density
sequence-density=1.47
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=37
prefix-density=1.45
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=162.07
fanout-score-rank=1
prefix-density=0.43
prefix-fanout=2.5
sequence=TTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGACACTTATATTTCGCTCTCACCTAATATCCACATTACTATGCCTGGAAGGCATAGTATTATCCTTATTTATTATAACTTCAGTAACTTCCCTAAACTCCAACTCCATAAGCTCCATACCAATCCCCATCACCATCTTAGTTTTCGCAGCCTGCGAAGCAGCTGTAGGACTAGCCCTACTAGTAAAAGTTTCAAACACGTACGGAACAGATTACGTCCAAAATCTCAACCTACTACAATGCTAAAAATTATTCTTCCCTC


criterion=sequence-density
sequence-density=1.41
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=37
prefix-density=1.40
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=170.79
fanout-score-rank=1
prefix-density=0.43
prefix-fanout=2.5
sequence=TTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGACACTTATATTTCGCTCTCACCTAATATCCACATTACTATGCCTGGAAGGCATAGTATTATCCTTATTTATTATAACTTCAGTAACTTCCCTAAACTCCAACTCCATAAGCTCCATACCAATCCCCATCACCATCTTAGTTTTCGCAGCCTGCGAAGCAGCTGTAGGACTAGCCCTACTAGTAAAAGTTTCAAACACGTACGGAACAGATTACGTCCAAAATCTCAACCTACTACAATGCTAAAAATTATTCTTCCCTC
SRR25558760 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 15:40:32
                             Started mapping on |	May 24 15:40:33
                                    Finished on |	May 24 15:50:43
       Mapping speed, Million of reads per hour |	232.81

                          Number of input reads |	39447903
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	34665515
                        Uniquely mapped reads % |	87.88%
                          Average mapped length |	298.91
                       Number of splices: Total |	38075379
            Number of splices: Annotated (sjdb) |	37851413
                       Number of splices: GT/AG |	37806695
                       Number of splices: GC/AG |	228122
                       Number of splices: AT/AC |	14892
               Number of splices: Non-canonical |	25670
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.62
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2611526
             % of reads mapped to multiple loci |	6.62%
        Number of reads mapped to too many loci |	29438
             % of reads mapped to too many loci |	0.07%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.24%
                     % of reads unmapped: other |	4.19%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2170862	2170862	2170862
N_multimapping	2611526	2611526	2611526
N_noFeature	1220442	17297328	17298851
N_ambiguous	2420476	610121	602143
UnstrandedReadsAssigned:31024597 PositiveStrandReadsAssigned:16758066 NegativeStrandReadsAssigned:16764521
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25558760 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558760-trimmed-pair1.fastq
                             SRR25558760-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 39,447,903 reads, 37,229,779 reads pseudoaligned
[quant] estimated average fragment length: 235.589
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,303 rounds

 109283 SRR25558760.ke.tsv
  52636 SRR25558760.se.tsv
 161919 total
==> SRR25558760.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	14.8	0	0
ENSMUST00000196221.1	9	7.8	0	0
ENSMUST00000179664.1	11	9.8	0	0
ENSMUST00000178537.1	12	10.8	0	0
ENSMUST00000178862.1	14	12.8	0	0
ENSMUST00000179520.1	11	9.8	0	0
ENSMUST00000179883.1	16	14.8	0	0
ENSMUST00000195858.1	10	8.8	0	0
ENSMUST00000179932.1	12	10.8	0	0

==> SRR25558760.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558760 completed mapping pipeline successfully
