Starting /dee2/code/volunteer_pipeline.sh SRR25558759
    current disk space = 1135872124
    free memory = 185673088 
SRR25558759 SRAfilesize
3d6e9fbeae788a260586ad230b48cc24  SRR25558759.sra
SRR25558759.sra file validated
SRR25558759 is paired end
SRR25558759 is conventional basespace
SRR25558759 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.481	37.0	37.0	37.0	37.0	37.0
2	36.42125	37.0	37.0	37.0	37.0	37.0
3	36.5245	37.0	37.0	37.0	37.0	37.0
4	36.415	37.0	37.0	37.0	37.0	37.0
5	36.5515	37.0	37.0	37.0	37.0	37.0
6	36.3685	37.0	37.0	37.0	37.0	37.0
7	36.5525	37.0	37.0	37.0	37.0	37.0
8	36.553	37.0	37.0	37.0	37.0	37.0
9	36.558	37.0	37.0	37.0	37.0	37.0
10-14	36.533	37.0	37.0	37.0	37.0	37.0
15-19	36.4915	37.0	37.0	37.0	37.0	37.0
20-24	36.4884	37.0	37.0	37.0	37.0	37.0
25-29	36.4256	37.0	37.0	37.0	37.0	37.0
30-34	36.3865	37.0	37.0	37.0	37.0	37.0
35-39	36.3863	37.0	37.0	37.0	37.0	37.0
40-44	36.3928	37.0	37.0	37.0	37.0	37.0
45-49	36.4348	37.0	37.0	37.0	37.0	37.0
50-54	36.320100000000004	37.0	37.0	37.0	37.0	37.0
55-59	36.3104	37.0	37.0	37.0	37.0	37.0
60-64	36.3909	37.0	37.0	37.0	37.0	37.0
65-69	36.3324	37.0	37.0	37.0	37.0	37.0
70-74	36.317	37.0	37.0	37.0	37.0	37.0
75-79	36.2964	37.0	37.0	37.0	37.0	37.0
80-84	36.2436	37.0	37.0	37.0	37.0	37.0
85-89	36.2136	37.0	37.0	37.0	37.0	37.0
90-94	36.250600000000006	37.0	37.0	37.0	37.0	37.0
95-99	36.2255	37.0	37.0	37.0	37.0	37.0
100-104	36.1984	37.0	37.0	37.0	37.0	37.0
105-109	36.087900000000005	37.0	37.0	37.0	37.0	37.0
110-114	36.1355	37.0	37.0	37.0	37.0	37.0
115-119	36.1674	37.0	37.0	37.0	37.0	37.0
120-124	36.0587	37.0	37.0	37.0	37.0	37.0
125-129	35.961299999999994	37.0	37.0	37.0	37.0	37.0
130-134	35.9499	37.0	37.0	37.0	37.0	37.0
135-139	35.9837	37.0	37.0	37.0	37.0	37.0
140-144	35.8615	37.0	37.0	37.0	37.0	37.0
145-149	35.9058	37.0	37.0	37.0	37.0	37.0
150	35.792	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	2.0
25	1.0
26	2.0
27	4.0
28	6.0
29	21.0
30	38.0
31	39.0
32	50.0
33	76.0
34	123.0
35	293.0
36	2935.0
37	409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.55	17.150000000000002	16.3	36.0
2	22.453066332916144	24.055068836045056	34.36795994993742	19.123904881101378
3	23.825	27.775	25.224999999999998	23.175
4	25.575	34.075	20.674999999999997	19.675
5	23.724999999999998	35.975	21.575	18.725
6	18.525	37.175000000000004	23.925	20.375
7	17.45	15.75	44.1	22.7
8	19.325	22.025	30.65	28.000000000000004
9	21.325	22.775000000000002	30.0	25.900000000000002
10-14	22.15	28.845	25.56	23.445
15-19	22.215	26.505000000000003	27.450000000000003	23.830000000000002
20-24	21.795	28.544999999999998	27.02	22.64
25-29	23.615	26.865	26.064999999999998	23.455000000000002
30-34	21.740000000000002	27.715	27.07	23.474999999999998
35-39	23.125	27.095000000000002	26.455000000000002	23.325000000000003
40-44	23.380000000000003	25.919999999999998	27.3	23.400000000000002
45-49	22.205	28.4	26.405	22.99
50-54	22.564999999999998	28.565	27.185	21.685
55-59	21.654999999999998	26.474999999999998	28.860000000000003	23.01
60-64	23.155	28.265	26.805	21.775
65-69	21.85	27.6	28.000000000000004	22.55
70-74	22.08	27.125	27.894999999999996	22.900000000000002
75-79	22.63	28.18	26.875	22.314999999999998
80-84	22.735	27.439999999999998	27.915	21.91
85-89	22.675	27.905	27.435	21.985
90-94	22.919999999999998	28.49	26.165	22.425
95-99	21.385	28.425	28.994999999999997	21.195
100-104	21.52	28.139999999999997	28.095	22.245
105-109	21.92	27.365000000000002	28.275	22.439999999999998
110-114	21.97	27.125	27.805000000000003	23.1
115-119	22.040000000000003	28.815	27.560000000000002	21.584999999999997
120-124	23.01	27.474999999999998	28.16	21.355
125-129	22.040000000000003	28.07	28.854999999999997	21.035
130-134	20.965	28.055000000000003	28.360000000000003	22.62
135-139	22.185	26.355	29.29	22.17
140-144	21.605	28.64	27.355	22.400000000000002
145-149	22.75	28.499999999999996	25.89	22.86
150	21.7	33.0	26.35	18.95
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	2.5
20	1.5
21	0.0
22	0.0
23	1.0
24	2.0
25	5.0
26	9.0
27	23.5
28	67.5
29	70.0
30	36.0
31	23.5
32	23.0
33	25.0
34	29.5
35	35.0
36	50.0
37	80.0
38	98.5
39	171.5
40	223.0
41	222.0
42	227.5
43	211.0
44	218.5
45	199.5
46	234.5
47	271.5
48	233.5
49	180.0
50	116.0
51	106.5
52	118.0
53	118.5
54	96.5
55	65.5
56	45.5
57	33.5
58	29.0
59	21.0
60	10.5
61	10.0
62	10.0
63	8.0
64	20.5
65	31.0
66	22.5
67	22.0
68	23.5
69	19.5
70	25.0
71	26.5
72	20.0
73	12.0
74	3.0
75	0.0
76	3.0
77	3.5
78	1.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.074999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.24126455906821	24.9
2	19.633943427620633	17.7
3	9.262340543538548	12.525
4	6.100942872989462	11.0
5	2.995008319467554	6.75
6	2.0521353300055463	5.55
7	1.4420410427066	4.55
8	0.776483638380477	2.8000000000000003
9	0.776483638380477	3.15
>10	1.7193566278424846	11.075
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	36	0.8999999999999999	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	23	0.575	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	20	0.5	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	19	0.475	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	17	0.42500000000000004	No Hit
GTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGG	17	0.42500000000000004	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	17	0.42500000000000004	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	17	0.42500000000000004	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	16	0.4	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	16	0.4	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	15	0.375	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	15	0.375	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	14	0.35000000000000003	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	13	0.325	No Hit
GCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAAT	12	0.3	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	12	0.3	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	12	0.3	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	12	0.3	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	12	0.3	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	12	0.3	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	11	0.27499999999999997	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	11	0.27499999999999997	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	11	0.27499999999999997	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	11	0.27499999999999997	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	11	0.27499999999999997	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	11	0.27499999999999997	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	10	0.25	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	10	0.25	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	10	0.25	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	10	0.25	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	10	0.25	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	9	0.22499999999999998	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	9	0.22499999999999998	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	9	0.22499999999999998	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	9	0.22499999999999998	No Hit
CTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTGG	9	0.22499999999999998	No Hit
GCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGAT	9	0.22499999999999998	No Hit
TGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAAC	9	0.22499999999999998	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	9	0.22499999999999998	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	9	0.22499999999999998	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	9	0.22499999999999998	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	9	0.22499999999999998	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	9	0.22499999999999998	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	9	0.22499999999999998	No Hit
CATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATA	9	0.22499999999999998	No Hit
AGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAA	8	0.2	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	8	0.2	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	8	0.2	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	8	0.2	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	8	0.2	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	8	0.2	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	8	0.2	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	8	0.2	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	8	0.2	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	8	0.2	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	8	0.2	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	8	0.2	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	8	0.2	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	8	0.2	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	7	0.17500000000000002	No Hit
CGGAAATATCTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCC	7	0.17500000000000002	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	7	0.17500000000000002	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	7	0.17500000000000002	No Hit
CGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGA	7	0.17500000000000002	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	7	0.17500000000000002	No Hit
AAACATTTTAACACTGCACACATTAGAAGTTCTATGGTCAGTCATGGGAA	7	0.17500000000000002	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	7	0.17500000000000002	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	7	0.17500000000000002	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	7	0.17500000000000002	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	7	0.17500000000000002	No Hit
CACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATAA	7	0.17500000000000002	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	7	0.17500000000000002	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	7	0.17500000000000002	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	7	0.17500000000000002	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	7	0.17500000000000002	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	7	0.17500000000000002	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	7	0.17500000000000002	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	7	0.17500000000000002	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	7	0.17500000000000002	No Hit
CTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCGATCAA	7	0.17500000000000002	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	7	0.17500000000000002	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	7	0.17500000000000002	No Hit
GAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAG	7	0.17500000000000002	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	7	0.17500000000000002	No Hit
CTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTA	7	0.17500000000000002	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	6	0.15	No Hit
AAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAGGA	6	0.15	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	6	0.15	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	6	0.15	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	6	0.15	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	6	0.15	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	6	0.15	No Hit
GGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGA	6	0.15	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	6	0.15	No Hit
TCGGGCTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTT	6	0.15	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	6	0.15	No Hit
TTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTC	6	0.15	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	6	0.15	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	6	0.15	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	6	0.15	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	6	0.15	No Hit
GCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCC	6	0.15	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	6	0.15	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	6	0.15	No Hit
AATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGT	6	0.15	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	6	0.15	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	6	0.15	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	6	0.15	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	6	0.15	No Hit
CCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAA	6	0.15	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	6	0.15	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	6	0.15	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	6	0.15	No Hit
CGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAG	6	0.15	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	6	0.15	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	6	0.15	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	6	0.15	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	6	0.15	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	6	0.15	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	6	0.15	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	6	0.15	No Hit
CTCCTACCTCCAATTGATTGACATCACTAAGAGGCCTTGAGTAGAAGTTC	6	0.15	No Hit
CAGAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCT	5	0.125	No Hit
GTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAG	5	0.125	No Hit
AAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCAT	5	0.125	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	5	0.125	No Hit
CACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGAT	5	0.125	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	5	0.125	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	5	0.125	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	5	0.125	No Hit
TTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTC	5	0.125	No Hit
TGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTG	5	0.125	No Hit
CAGACATCTGAATTTCAGCCTTCCTATTTCCCCAAGAGGTCTTGGCGCCA	5	0.125	No Hit
GGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTC	5	0.125	No Hit
TTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGA	5	0.125	No Hit
GGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCG	5	0.125	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	5	0.125	No Hit
GGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAGGCCTCT	5	0.125	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	5	0.125	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	5	0.125	No Hit
GCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCC	5	0.125	No Hit
CACAGTAGTAGAGTATAAACCTCTTCCCATGACTGACCATAGAACTTCTA	5	0.125	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	5	0.125	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	5	0.125	No Hit
TGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTG	5	0.125	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	5	0.125	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	5	0.125	No Hit
ATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAA	5	0.125	No Hit
GTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCACTTACATCTTACC	5	0.125	No Hit
GCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCG	5	0.125	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	5	0.125	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	5	0.125	No Hit
ACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	5	0.125	No Hit
GCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCA	5	0.125	No Hit
TCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTAC	5	0.125	No Hit
GAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCA	5	0.125	No Hit
TGCCTTCATAGATTTTGAAGGTTACATTGGCTGGATTTATCAATGCTTTT	5	0.125	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	5	0.125	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	5	0.125	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	5	0.125	No Hit
ATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTC	5	0.125	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	5	0.125	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	5	0.125	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	5	0.125	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	5	0.125	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	5	0.125	No Hit
CGCGGTTATTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAG	5	0.125	No Hit
CCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGC	5	0.125	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	5	0.125	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	5	0.125	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	5	0.125	No Hit
TGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACG	5	0.125	No Hit
AACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAAAAC	5	0.125	No Hit
CAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCA	5	0.125	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	5	0.125	No Hit
GTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25558759 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558759_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3905	37.0	37.0	37.0	37.0	37.0
2	36.3725	37.0	37.0	37.0	37.0	37.0
3	36.455	37.0	37.0	37.0	37.0	37.0
4	36.358	37.0	37.0	37.0	37.0	37.0
5	36.3005	37.0	37.0	37.0	37.0	37.0
6	36.399	37.0	37.0	37.0	37.0	37.0
7	36.3665	37.0	37.0	37.0	37.0	37.0
8	36.3215	37.0	37.0	37.0	37.0	37.0
9	36.453	37.0	37.0	37.0	37.0	37.0
10-14	36.355000000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.393	37.0	37.0	37.0	37.0	37.0
20-24	36.29879999999999	37.0	37.0	37.0	37.0	37.0
25-29	36.249900000000004	37.0	37.0	37.0	37.0	37.0
30-34	36.2944	37.0	37.0	37.0	37.0	37.0
35-39	36.2243	37.0	37.0	37.0	37.0	37.0
40-44	36.28920000000001	37.0	37.0	37.0	37.0	37.0
45-49	36.1893	37.0	37.0	37.0	37.0	37.0
50-54	36.1367	37.0	37.0	37.0	37.0	37.0
55-59	36.1405	37.0	37.0	37.0	37.0	37.0
60-64	36.1323	37.0	37.0	37.0	37.0	37.0
65-69	36.119749999999996	37.0	37.0	37.0	37.0	37.0
70-74	36.0512	37.0	37.0	37.0	37.0	37.0
75-79	35.966899999999995	37.0	37.0	37.0	37.0	37.0
80-84	35.9816	37.0	37.0	37.0	37.0	37.0
85-89	35.9247	37.0	37.0	37.0	37.0	37.0
90-94	35.9119	37.0	37.0	37.0	37.0	37.0
95-99	35.7999	37.0	37.0	37.0	37.0	37.0
100-104	35.7572	37.0	37.0	37.0	37.0	37.0
105-109	35.811400000000006	37.0	37.0	37.0	37.0	37.0
110-114	35.751	37.0	37.0	37.0	37.0	37.0
115-119	35.64020000000001	37.0	37.0	37.0	37.0	37.0
120-124	35.5382	37.0	37.0	37.0	37.0	37.0
125-129	35.509699999999995	37.0	37.0	37.0	37.0	37.0
130-134	35.570299999999996	37.0	37.0	37.0	37.0	37.0
135-139	35.4791	37.0	37.0	37.0	37.0	37.0
140-144	35.4665	37.0	37.0	37.0	34.6	37.0
145-149	35.24810000000001	37.0	37.0	37.0	27.4	37.0
150	35.19	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	2.0
21	2.0
22	2.0
23	4.0
24	2.0
25	3.0
26	8.0
27	11.0
28	17.0
29	20.0
30	25.0
31	47.0
32	62.0
33	68.0
34	172.0
35	588.0
36	2795.0
37	170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.825000000000003	15.75	15.950000000000001	38.475
2	20.4	25.6	34.75	19.25
3	27.625	26.6	24.925	20.849999999999998
4	25.05	35.05	21.15	18.75
5	24.325	36.975	19.725	18.975
6	18.9	37.974999999999994	22.95	20.175
7	18.7	16.45	42.475	22.375
8	19.475	22.025	29.099999999999998	29.4
9	20.925	23.3	28.749999999999996	27.025
10-14	22.665	27.985	26.295	23.055
15-19	22.355	26.325	27.13	24.19
20-24	21.84	27.779999999999998	27.74	22.64
25-29	22.175	27.33	25.655	24.84
30-34	22.15	27.365000000000002	26.790000000000003	23.695
35-39	22.945	27.46	26.11	23.485
40-44	23.880000000000003	26.135	27.325	22.66
45-49	22.220000000000002	28.7	25.69	23.39
50-54	22.720000000000002	27.62	27.355	22.305
55-59	21.349999999999998	26.174999999999997	28.92	23.555
60-64	23.18	27.375	27.134999999999998	22.31
65-69	21.861093054652734	27.60638031901595	28.521426071303562	22.011100555027753
70-74	21.975	27.625	28.194999999999997	22.205
75-79	23.380000000000003	28.73	26.445	21.445
80-84	22.285	27.765	28.875	21.075
85-89	22.78	28.244999999999997	27.485	21.490000000000002
90-94	23.23	28.794999999999998	26.02	21.955
95-99	21.92	29.14	28.299999999999997	20.64
100-104	21.97	27.779999999999998	27.925	22.325
105-109	21.675	27.685	27.855	22.785
110-114	21.305	26.505000000000003	28.825	23.365
115-119	21.305	27.875	28.38	22.439999999999998
120-124	21.965	27.025	29.145	21.865000000000002
125-129	21.67	27.93	29.095	21.305
130-134	21.58	27.605	29.549999999999997	21.265
135-139	22.86	27.115000000000002	28.83	21.195
140-144	21.790000000000003	28.04	27.584999999999997	22.585
145-149	22.595000000000002	28.95	26.21	22.245
150	21.55	30.825000000000003	27.675	19.950000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	3.0
21	2.5
22	0.5
23	1.5
24	3.5
25	4.0
26	8.5
27	25.0
28	68.5
29	78.5
30	44.5
31	25.0
32	21.0
33	22.5
34	27.5
35	30.5
36	49.0
37	78.0
38	87.0
39	135.5
40	195.5
41	219.0
42	218.0
43	215.5
44	230.5
45	200.0
46	220.0
47	262.5
48	233.5
49	171.0
50	129.0
51	128.5
52	138.0
53	143.0
54	116.0
55	83.5
56	56.0
57	37.5
58	25.5
59	17.0
60	13.5
61	11.5
62	11.5
63	12.0
64	18.5
65	26.0
66	22.5
67	26.5
68	21.5
69	8.0
70	15.0
71	21.5
72	15.5
73	8.5
74	4.5
75	2.0
76	0.0
77	0.0
78	1.0
79	1.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.005
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.433001107419706	24.125
2	20.81949058693245	18.8
3	10.520487264673312	14.249999999999998
4	6.533776301218161	11.799999999999999
5	2.7131782945736433	6.125
6	1.716500553709856	4.65
7	1.1627906976744187	3.675
8	0.6090808416389812	2.1999999999999997
9	0.7198228128460686	2.9250000000000003
>10	1.716500553709856	10.174999999999999
>50	0.05537098560354374	1.275
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	51	1.275	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	26	0.65	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	24	0.6	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	24	0.6	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	23	0.575	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	17	0.42500000000000004	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	15	0.375	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	14	0.35000000000000003	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	14	0.35000000000000003	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	13	0.325	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	13	0.325	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	13	0.325	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	13	0.325	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	13	0.325	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	12	0.3	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	11	0.27499999999999997	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	11	0.27499999999999997	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	11	0.27499999999999997	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	10	0.25	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	10	0.25	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	10	0.25	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	10	0.25	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	10	0.25	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	10	0.25	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	10	0.25	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	10	0.25	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	10	0.25	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	10	0.25	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	10	0.25	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	10	0.25	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	10	0.25	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	10	0.25	No Hit
AAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAA	9	0.22499999999999998	No Hit
CTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTCGATTA	9	0.22499999999999998	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	9	0.22499999999999998	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	9	0.22499999999999998	No Hit
TTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAAT	9	0.22499999999999998	No Hit
GCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGC	9	0.22499999999999998	No Hit
CAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGC	9	0.22499999999999998	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	9	0.22499999999999998	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	9	0.22499999999999998	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	9	0.22499999999999998	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	9	0.22499999999999998	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	9	0.22499999999999998	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	9	0.22499999999999998	No Hit
TATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGC	8	0.2	No Hit
CGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGG	8	0.2	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	8	0.2	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	8	0.2	No Hit
GGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAG	8	0.2	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	8	0.2	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	8	0.2	No Hit
GCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCG	8	0.2	No Hit
GTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGG	8	0.2	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	8	0.2	No Hit
GGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAG	8	0.2	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	7	0.17500000000000002	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	7	0.17500000000000002	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	7	0.17500000000000002	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	7	0.17500000000000002	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	7	0.17500000000000002	No Hit
GTAGAGTATAAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTG	7	0.17500000000000002	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	7	0.17500000000000002	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	7	0.17500000000000002	No Hit
GAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAG	7	0.17500000000000002	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	7	0.17500000000000002	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	7	0.17500000000000002	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	7	0.17500000000000002	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	7	0.17500000000000002	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	7	0.17500000000000002	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	7	0.17500000000000002	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	7	0.17500000000000002	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	7	0.17500000000000002	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	7	0.17500000000000002	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	7	0.17500000000000002	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	7	0.17500000000000002	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	7	0.17500000000000002	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	6	0.15	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	6	0.15	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	6	0.15	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	6	0.15	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	6	0.15	No Hit
ATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTA	6	0.15	No Hit
GCCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	6	0.15	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	6	0.15	No Hit
CTTCGGGCTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATAT	6	0.15	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	6	0.15	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	6	0.15	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	6	0.15	No Hit
CTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAA	6	0.15	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	6	0.15	No Hit
GGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACA	6	0.15	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	6	0.15	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	6	0.15	No Hit
TGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATAGCCAT	6	0.15	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	6	0.15	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	6	0.15	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	6	0.15	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	6	0.15	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	6	0.15	No Hit
TTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCATA	6	0.15	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	6	0.15	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	6	0.15	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	6	0.15	No Hit
GTGTATTGAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCA	6	0.15	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	6	0.15	No Hit
CAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCA	6	0.15	No Hit
GTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGG	6	0.15	No Hit
CCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTATGCC	5	0.125	No Hit
CCTACCTCCAATTGATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCAC	5	0.125	No Hit
GCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGC	5	0.125	No Hit
TGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTC	5	0.125	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	5	0.125	No Hit
CTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGT	5	0.125	No Hit
TGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTC	5	0.125	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	5	0.125	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	5	0.125	No Hit
TCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCAC	5	0.125	No Hit
CGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGA	5	0.125	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	5	0.125	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	5	0.125	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	5	0.125	No Hit
TTTCACCTTTACATCCCCGAGGGGGCTCAGGGACTCGGCCCAGGGACAGG	5	0.125	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	5	0.125	No Hit
TGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCAT	5	0.125	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	5	0.125	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	5	0.125	No Hit
GCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGG	5	0.125	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	5	0.125	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	5	0.125	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	5	0.125	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	5	0.125	No Hit
AGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCAC	5	0.125	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	5	0.125	No Hit
TGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAAC	5	0.125	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	5	0.125	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	5	0.125	No Hit
CAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGG	5	0.125	No Hit
GCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCA	5	0.125	No Hit
GACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAG	5	0.125	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	5	0.125	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	5	0.125	No Hit
GTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAA	5	0.125	No Hit
TAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGC	5	0.125	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	5	0.125	No Hit
TCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTT	5	0.125	No Hit
CTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCGATCAA	5	0.125	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	5	0.125	No Hit
CGGGCTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTC	5	0.125	No Hit
CAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACT	5	0.125	No Hit
GAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAG	5	0.125	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	5	0.125	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	5	0.125	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	5	0.125	No Hit
CACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGA	5	0.125	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	5	0.125	No Hit
TGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	10	0.006973645	144.0	6
TGGTAAC	10	0.006973645	144.0	1
TAACAGA	10	0.006973645	144.0	4
AACAGAC	10	0.006973645	144.0	5
GTAACAG	10	0.006973645	144.0	3
>>END_MODULE
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929174 spots for SRR25558759.sra
Written 4929174 spots for SRR25558759.sra
Read 4929175 spots for SRR25558759.sra
Written 4929175 spots for SRR25558759.sra
SRR ids: ['SRR25558759.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_y20vnh9w
SRR25558759.sra spots: 39433393
blocks: [[1, 4929174], [4929175, 9858348], [9858349, 14787522], [14787523, 19716696], [19716697, 24645870], [24645871, 29575044], [29575045, 34504218], [34504219, 39433393]]
SRR25558759 file size 13302500
SRR25558759 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25558759 SRR25558759_1.fastq SRR25558759_2.fastq
Input file:	SRR25558759_1.fastq
Paired file:	SRR25558759_2.fastq
trimmed:	SRR25558759-trimmed-pair1.fastq, SRR25558759-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun May 24 15:29:01 2026 >> started

Sun May 24 15:30:11 2026 >> done (70.083s)
39433393 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
39433392 (100.00%) read pairs available; of these:
  401557 ( 1.02%) trimmed read pairs available after processing
39031835 (98.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 48	       1	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       1	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       1	  0.00%
 65	       2	  0.00%
 66	       1	  0.00%
 67	       2	  0.00%
 68	       5	  0.00%
 69	       4	  0.00%
 70	       4	  0.00%
 71	       6	  0.00%
 72	       5	  0.00%
 73	       8	  0.00%
 74	       5	  0.00%
 75	       6	  0.00%
 76	       9	  0.00%
 77	       4	  0.00%
 78	       6	  0.00%
 79	      12	  0.00%
 80	      18	  0.00%
 81	       8	  0.00%
 82	      11	  0.00%
 83	      11	  0.00%
 84	      16	  0.00%
 85	      13	  0.00%
 86	      15	  0.00%
 87	      19	  0.00%
 88	      26	  0.00%
 89	      17	  0.00%
 90	      24	  0.00%
 91	      36	  0.00%
 92	      31	  0.00%
 93	      33	  0.00%
 94	      40	  0.00%
 95	      53	  0.00%
 96	      63	  0.00%
 97	      62	  0.00%
 98	      64	  0.00%
 99	      67	  0.00%
100	      64	  0.00%
101	      78	  0.00%
102	      70	  0.00%
103	      99	  0.00%
104	     100	  0.00%
105	     136	  0.00%
106	     136	  0.00%
107	     120	  0.00%
108	     129	  0.00%
109	     128	  0.00%
110	     140	  0.00%
111	     141	  0.00%
112	     161	  0.00%
113	     171	  0.00%
114	     179	  0.00%
115	     177	  0.00%
116	     223	  0.00%
117	     204	  0.00%
118	     226	  0.00%
119	     249	  0.00%
120	     240	  0.00%
121	     275	  0.00%
122	     272	  0.00%
123	     307	  0.00%
124	     337	  0.00%
125	     345	  0.00%
126	     342	  0.00%
127	     367	  0.00%
128	     428	  0.00%
129	     411	  0.00%
130	     441	  0.00%
131	     459	  0.00%
132	     500	  0.00%
133	     495	  0.00%
134	     572	  0.00%
135	     545	  0.00%
136	     598	  0.00%
137	     632	  0.00%
138	     605	  0.00%
139	     677	  0.00%
140	     707	  0.00%
141	   36936	  0.09%
142	   38431	  0.10%
143	   39704	  0.10%
144	   41657	  0.11%
145	   43142	  0.11%
146	   44986	  0.11%
147	   45971	  0.12%
148	   47810	  0.12%
149	   49725	  0.13%
150	39031835	 98.98%
39433392 reads passed initial QC


criterion=sequence-density
sequence-density=1.71
sequence-density-rank=1
fanout-score=1.98
fanout-score-rank=37
prefix-density=1.69
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=121.44
fanout-score-rank=1
prefix-density=1.63
prefix-fanout=1.1
sequence=CCTTCCTTTAATCTTAAGTAAAAGAGACACAGGGATTCAAAAATAAAAATTTCTTTCCCCATTCCCAGGCCTGTACCCAGTGCCCTCCATACCACCCTTCCCCTCTCTAACAGAAGCAAGGGAGGTTCAGCTTAACAGCCGCTGGGGGGGGTCAGAGGGGGGCTTCTGAGCTCAGTGTTGGTCTCTTTCCAAATATAAATACATGTGTCAAAACTGGGAACTCCTCCACACCCGTCACCCTGACGCCCTCCATTTCTGCTGGTGTTCGGGATGGGGAAGCCAGGCACCGACTGGCTGGGGTTTACTGCACACTTTGGGCATGGGCCCCACCAGTCTCCTGCTGCTCGTTGTAGAAGAGATGGCACTCGGGGTCTCCCCGGATGGTGGGAGCTCCCTGGATGGGCTTCCCGGTATTGGGGTTCACACACCAGCACTCCCCGCGCTGTCCGTTCAGAGACATCTTGCACTGCTTAAGGTTGTACCGGCCATGCTTGTCACAGTTGGGGATGTG


criterion=sequence-density
sequence-density=1.96
sequence-density-rank=1
fanout-score=1.94
fanout-score-rank=38
prefix-density=1.97
prefix-fanout=1.9
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=31
fanout-score=68.35
fanout-score-rank=1
prefix-density=2.23
prefix-fanout=1.1
sequence=CATCATGATGCTTGTGTATTGTTATTTTTTTTTGGCAATGATTCTCACTTATATAGTCAACCTAGAAATAGCATTTTTTCTTCCCATGATTCACAAAGTGAGATTGGATTTCTTTGATAGATCCTTGCTTCAGGTTTTAGAAGATGTCAGCAGATGACAGAGGACACAGAAGTGGTTATTTTCTTGATATTCATGTTGGATTTTAATATAAGTCAAGTGAAATGTATAGGCTGATTCACCTTCTGCTCCAATTTGGAAACCCTGTAGAAGTTCCTGTTCCTTTTGTAGGATGGATTCTGGCAGTAGCCTGACTCTCACCTTGCATTCTGTATACCACACATATTACACATAGGGCCTCCATTCCTTTACATGCAACAT
SRR25558759 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 15:30:28
                             Started mapping on |	May 24 15:30:28
                                    Finished on |	May 24 15:35:25
       Mapping speed, Million of reads per hour |	477.98

                          Number of input reads |	39433392
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	36367261
                        Uniquely mapped reads % |	92.22%
                          Average mapped length |	298.86
                       Number of splices: Total |	37997925
            Number of splices: Annotated (sjdb) |	37778825
                       Number of splices: GT/AG |	37719361
                       Number of splices: GC/AG |	233502
                       Number of splices: AT/AC |	18198
               Number of splices: Non-canonical |	26864
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.61
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2422562
             % of reads mapped to multiple loci |	6.14%
        Number of reads mapped to too many loci |	42483
             % of reads mapped to too many loci |	0.11%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.42%
                     % of reads unmapped: other |	0.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	643569	643569	643569
N_multimapping	2422562	2422562	2422562
N_noFeature	1793161	18355556	18430684
N_ambiguous	2803667	766810	754590
UnstrandedReadsAssigned:31770433 PositiveStrandReadsAssigned:17244895 NegativeStrandReadsAssigned:17181987
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25558759 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558759-trimmed-pair1.fastq
                             SRR25558759-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 39,433,392 reads, 36,556,837 reads pseudoaligned
[quant] estimated average fragment length: 239.357
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,078 rounds

 109283 SRR25558759.ke.tsv
  52636 SRR25558759.se.tsv
 161919 total
==> SRR25558759.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9.33333	0	0
ENSMUST00000196221.1	9	9	0	0
ENSMUST00000179664.1	11	4.33333	0	0
ENSMUST00000178537.1	12	5.33333	0	0
ENSMUST00000178862.1	14	7.33333	0	0
ENSMUST00000179520.1	11	4.33333	0	0
ENSMUST00000179883.1	16	9.33333	0	0
ENSMUST00000195858.1	10	10	0	0
ENSMUST00000179932.1	12	5.33333	0	0

==> SRR25558759.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558759 completed mapping pipeline successfully
