Starting /dee2/code/volunteer_pipeline.sh SRR25558761
    current disk space = 1153902348
    free memory = 187217152 
SRR25558761 SRAfilesize
b93e08f9c1eec6692b7289daabf4dcbb  SRR25558761.sra
SRR25558761.sra file validated
SRR25558761 is paired end
SRR25558761 is conventional basespace
SRR25558761 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558761_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.461	37.0	37.0	37.0	37.0	37.0
2	36.33875	37.0	37.0	37.0	37.0	37.0
3	36.4805	37.0	37.0	37.0	37.0	37.0
4	36.3645	37.0	37.0	37.0	37.0	37.0
5	36.4935	37.0	37.0	37.0	37.0	37.0
6	36.422	37.0	37.0	37.0	37.0	37.0
7	36.3325	37.0	37.0	37.0	37.0	37.0
8	36.4415	37.0	37.0	37.0	37.0	37.0
9	36.495	37.0	37.0	37.0	37.0	37.0
10-14	36.478699999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.469899999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.4502	37.0	37.0	37.0	37.0	37.0
25-29	36.39200000000001	37.0	37.0	37.0	37.0	37.0
30-34	36.3779	37.0	37.0	37.0	37.0	37.0
35-39	36.393	37.0	37.0	37.0	37.0	37.0
40-44	36.28589999999999	37.0	37.0	37.0	37.0	37.0
45-49	36.3296	37.0	37.0	37.0	37.0	37.0
50-54	36.2112	37.0	37.0	37.0	37.0	37.0
55-59	36.2512	37.0	37.0	37.0	37.0	37.0
60-64	36.1976	37.0	37.0	37.0	37.0	37.0
65-69	36.2186	37.0	37.0	37.0	37.0	37.0
70-74	36.2112	37.0	37.0	37.0	37.0	37.0
75-79	36.246900000000004	37.0	37.0	37.0	37.0	37.0
80-84	36.196999999999996	37.0	37.0	37.0	37.0	37.0
85-89	36.167	37.0	37.0	37.0	37.0	37.0
90-94	36.1416	37.0	37.0	37.0	37.0	37.0
95-99	36.1397	37.0	37.0	37.0	37.0	37.0
100-104	36.137	37.0	37.0	37.0	37.0	37.0
105-109	36.0432	37.0	37.0	37.0	37.0	37.0
110-114	36.0972	37.0	37.0	37.0	37.0	37.0
115-119	36.0545	37.0	37.0	37.0	37.0	37.0
120-124	36.0146	37.0	37.0	37.0	37.0	37.0
125-129	35.928999999999995	37.0	37.0	37.0	37.0	37.0
130-134	35.9257	37.0	37.0	37.0	37.0	37.0
135-139	36.0169	37.0	37.0	37.0	37.0	37.0
140-144	35.989999999999995	37.0	37.0	37.0	37.0	37.0
145-149	35.9326	37.0	37.0	37.0	37.0	37.0
150	35.8135	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	5.0
27	6.0
28	16.0
29	22.0
30	35.0
31	54.0
32	62.0
33	69.0
34	126.0
35	272.0
36	3023.0
37	307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.8	15.225	17.125	36.85
2	22.890057600801402	23.31580265464563	33.48359629351365	20.31054345103932
3	25.974999999999998	25.7	25.25	23.075000000000003
4	26.174999999999997	34.125	21.375	18.325
5	24.925	37.275000000000006	19.825	17.974999999999998
6	19.1	37.4	23.474999999999998	20.025000000000002
7	19.05	16.525000000000002	42.925000000000004	21.5
8	20.0	21.25	31.574999999999996	27.175
9	21.6	24.0	27.950000000000003	26.450000000000003
10-14	23.18	28.825	24.94	23.055
15-19	22.650000000000002	26.240000000000002	27.060000000000002	24.05
20-24	22.035	27.744999999999997	27.705000000000002	22.515
25-29	22.95	26.314999999999998	26.185000000000002	24.55
30-34	22.675	27.345000000000002	26.455000000000002	23.525
35-39	23.74	26.229999999999997	27.200000000000003	22.830000000000002
40-44	23.35	26.240000000000002	26.369999999999997	24.04
45-49	23.285	28.12	25.455	23.14
50-54	22.525000000000002	28.345	27.279999999999998	21.85
55-59	22.29	26.505000000000003	28.625	22.58
60-64	23.905	27.529999999999998	26.405	22.16
65-69	22.455	26.235000000000003	27.85	23.46
70-74	22.215	27.075	27.800000000000004	22.91
75-79	22.2	27.084999999999997	27.400000000000002	23.315
80-84	22.025	27.845	28.884999999999998	21.245
85-89	23.080000000000002	27.87	27.57	21.48
90-94	23.645	28.660000000000004	25.174999999999997	22.52
95-99	21.584999999999997	29.935000000000002	27.565	20.915
100-104	21.15	29.175	26.584999999999997	23.09
105-109	22.895	27.015	27.485	22.605
110-114	22.24	28.32	26.995	22.445
115-119	21.355	27.04	28.43	23.175
120-124	23.555	26.985	27.595	21.865000000000002
125-129	22.53	27.450000000000003	27.425	22.595000000000002
130-134	22.015	27.295	28.499999999999996	22.189999999999998
135-139	23.015	26.035000000000004	29.43	21.52
140-144	22.11	27.82	27.389999999999997	22.68
145-149	22.89	28.035	26.6	22.475
150	20.474999999999998	29.5	29.025000000000002	21.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	1.0
20	1.0
21	1.0
22	0.5
23	0.5
24	1.0
25	1.0
26	2.5
27	16.5
28	45.0
29	56.0
30	42.0
31	26.0
32	20.5
33	16.5
34	16.5
35	26.5
36	44.0
37	62.5
38	78.0
39	158.5
40	211.0
41	216.0
42	234.0
43	232.0
44	228.0
45	199.5
46	224.5
47	275.0
48	277.5
49	212.0
50	130.0
51	117.5
52	134.5
53	140.0
54	103.0
55	68.5
56	66.5
57	42.0
58	13.0
59	8.0
60	12.0
61	12.0
62	5.0
63	6.0
64	13.5
65	24.0
66	26.0
67	36.5
68	34.5
69	13.5
70	14.0
71	16.0
72	15.5
73	14.5
74	4.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	1.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.52459016393443	16.975
2	21.57377049180328	16.45
3	12.983606557377051	14.85
4	6.885245901639345	10.5
5	4.590163934426229	8.75
6	2.098360655737705	4.8
7	2.360655737704918	6.3
8	0.9180327868852459	2.8000000000000003
9	1.180327868852459	4.05
>10	2.8852459016393444	14.524999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	37	0.9249999999999999	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	22	0.5499999999999999	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	19	0.475	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	19	0.475	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	18	0.44999999999999996	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	18	0.44999999999999996	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	15	0.375	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	15	0.375	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	15	0.375	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	15	0.375	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	14	0.35000000000000003	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	14	0.35000000000000003	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	14	0.35000000000000003	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	13	0.325	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	13	0.325	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	13	0.325	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	13	0.325	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	13	0.325	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	13	0.325	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	13	0.325	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	13	0.325	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	12	0.3	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	11	0.27499999999999997	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	11	0.27499999999999997	No Hit
TATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAG	11	0.27499999999999997	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	11	0.27499999999999997	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	11	0.27499999999999997	No Hit
CAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCAT	11	0.27499999999999997	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	11	0.27499999999999997	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	11	0.27499999999999997	No Hit
TGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAAT	11	0.27499999999999997	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	11	0.27499999999999997	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	10	0.25	No Hit
TGCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCC	10	0.25	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	10	0.25	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	10	0.25	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	10	0.25	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	10	0.25	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	10	0.25	No Hit
ATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCT	10	0.25	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	10	0.25	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	10	0.25	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	10	0.25	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	10	0.25	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	9	0.22499999999999998	No Hit
CAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGC	9	0.22499999999999998	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	9	0.22499999999999998	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	9	0.22499999999999998	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	9	0.22499999999999998	No Hit
GCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCG	9	0.22499999999999998	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	9	0.22499999999999998	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	9	0.22499999999999998	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	9	0.22499999999999998	No Hit
TGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCG	9	0.22499999999999998	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	9	0.22499999999999998	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	9	0.22499999999999998	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	9	0.22499999999999998	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	9	0.22499999999999998	No Hit
CGCCCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGA	9	0.22499999999999998	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	9	0.22499999999999998	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	9	0.22499999999999998	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	9	0.22499999999999998	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	8	0.2	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	8	0.2	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	8	0.2	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	8	0.2	No Hit
CACCTACCTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAG	8	0.2	No Hit
TTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGC	8	0.2	No Hit
TCCTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTAC	8	0.2	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	8	0.2	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	8	0.2	No Hit
GGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGA	8	0.2	No Hit
CTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAAAACTGTTTGT	8	0.2	No Hit
AACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATAC	8	0.2	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	8	0.2	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	8	0.2	No Hit
GCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGT	7	0.17500000000000002	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	7	0.17500000000000002	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	7	0.17500000000000002	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	7	0.17500000000000002	No Hit
CTGAAATGAAGGAATCTCAATTAAGAAAATGCCTCTGTACAATCCAGCTG	7	0.17500000000000002	No Hit
ATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTG	7	0.17500000000000002	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	7	0.17500000000000002	No Hit
GAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCA	7	0.17500000000000002	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	7	0.17500000000000002	No Hit
CTGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATAGCCA	7	0.17500000000000002	No Hit
CGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCAT	7	0.17500000000000002	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	7	0.17500000000000002	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	7	0.17500000000000002	No Hit
GTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGA	7	0.17500000000000002	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	7	0.17500000000000002	No Hit
AAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATT	7	0.17500000000000002	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	7	0.17500000000000002	No Hit
ATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGC	7	0.17500000000000002	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	7	0.17500000000000002	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	7	0.17500000000000002	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	7	0.17500000000000002	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	7	0.17500000000000002	No Hit
GCCATATTCATATTTAATGTGACTGGCATCACAGGGGCATGTGGACAGAT	7	0.17500000000000002	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	7	0.17500000000000002	No Hit
CTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTT	7	0.17500000000000002	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	7	0.17500000000000002	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	7	0.17500000000000002	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	7	0.17500000000000002	No Hit
GCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGT	7	0.17500000000000002	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	7	0.17500000000000002	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	7	0.17500000000000002	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	7	0.17500000000000002	No Hit
GCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGG	7	0.17500000000000002	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	7	0.17500000000000002	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	6	0.15	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	6	0.15	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	6	0.15	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	6	0.15	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	6	0.15	No Hit
CTATAGTCTAACTCACAGAACTAAGAAGTCACTTACATCTTACCTGAACA	6	0.15	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	6	0.15	No Hit
TTTGACGGTTGCCGACCCGACCTGAGGGCCAGAAGCAGTGTTACACACTT	6	0.15	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	6	0.15	No Hit
GGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGACCA	6	0.15	No Hit
TGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAAT	6	0.15	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	6	0.15	No Hit
CAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGAC	6	0.15	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	6	0.15	No Hit
CACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGAT	6	0.15	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	6	0.15	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	6	0.15	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	6	0.15	No Hit
CTTTTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAA	6	0.15	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	6	0.15	No Hit
CTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGACACTTAAA	6	0.15	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	6	0.15	No Hit
GGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGC	6	0.15	No Hit
CTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTGG	6	0.15	No Hit
TGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCT	6	0.15	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	6	0.15	No Hit
ACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCACTCAGTTG	6	0.15	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	6	0.15	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	6	0.15	No Hit
AAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGAT	6	0.15	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	6	0.15	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	6	0.15	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	5	0.125	No Hit
GCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATG	5	0.125	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	5	0.125	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	5	0.125	No Hit
CAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTA	5	0.125	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	5	0.125	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	5	0.125	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	5	0.125	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	5	0.125	No Hit
TGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTC	5	0.125	No Hit
CCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGG	5	0.125	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	5	0.125	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	5	0.125	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	5	0.125	No Hit
CCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTT	5	0.125	No Hit
TTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAA	5	0.125	No Hit
GTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAAT	5	0.125	No Hit
CCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTC	5	0.125	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	5	0.125	No Hit
TTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCT	5	0.125	No Hit
CAAGGCTTTTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCT	5	0.125	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	5	0.125	No Hit
GAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTC	5	0.125	No Hit
CCATAGTTACATTACAGGATGTGTGCACACCGAAAGCTGAGGCCACATTC	5	0.125	No Hit
GGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGG	5	0.125	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	5	0.125	No Hit
CATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA	5	0.125	No Hit
AACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAG	5	0.125	No Hit
CTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCC	5	0.125	No Hit
AAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGG	5	0.125	No Hit
CTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATAAGCTCC	5	0.125	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	5	0.125	No Hit
AGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACA	5	0.125	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	5	0.125	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	5	0.125	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	5	0.125	No Hit
TGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGAT	5	0.125	No Hit
CAGAATTGTAAAACTGATGTGAAATGATTAATTGCTTCGGTAAAGTTCCT	5	0.125	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	5	0.125	No Hit
CGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGG	5	0.125	No Hit
TAGACACTTAAAAGGATCATATATTTCCCATGAGGGTAACATCATTTAGT	5	0.125	No Hit
CTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTA	5	0.125	No Hit
GTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCT	5	0.125	No Hit
CGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCC	5	0.125	No Hit
CCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCT	5	0.125	No Hit
TGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCG	5	0.125	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	5	0.125	No Hit
CATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGC	5	0.125	No Hit
TTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATT	5	0.125	No Hit
CTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCA	5	0.125	No Hit
TTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCATA	5	0.125	No Hit
GGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGC	5	0.125	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	5	0.125	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	5	0.125	No Hit
GGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCT	5	0.125	No Hit
TGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCA	5	0.125	No Hit
GCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGA	5	0.125	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	5	0.125	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	5	0.125	No Hit
CCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAG	5	0.125	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	5	0.125	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	5	0.125	No Hit
CAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGA	5	0.125	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	5	0.125	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	5	0.125	No Hit
GTGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACA	5	0.125	No Hit
CTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTT	5	0.125	No Hit
GAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTA	5	0.125	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	5	0.125	No Hit
CAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	10	0.006973645	144.0	6
TGGTAAC	10	0.006973645	144.0	1
TAACAGA	10	0.006973645	144.0	4
CAGACCC	10	0.006973645	144.0	7
AACAGAC	10	0.006973645	144.0	5
AGACCCA	10	0.006973645	144.0	8
GTAACAG	10	0.006973645	144.0	3
>>END_MODULE
SRR25558761 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558761_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3355	37.0	37.0	37.0	37.0	37.0
2	36.32	37.0	37.0	37.0	37.0	37.0
3	36.3765	37.0	37.0	37.0	37.0	37.0
4	36.3155	37.0	37.0	37.0	37.0	37.0
5	36.256	37.0	37.0	37.0	37.0	37.0
6	36.315	37.0	37.0	37.0	37.0	37.0
7	36.354	37.0	37.0	37.0	37.0	37.0
8	36.3035	37.0	37.0	37.0	37.0	37.0
9	36.312	37.0	37.0	37.0	37.0	37.0
10-14	36.351	37.0	37.0	37.0	37.0	37.0
15-19	36.3752	37.0	37.0	37.0	37.0	37.0
20-24	36.2594	37.0	37.0	37.0	37.0	37.0
25-29	36.2263	37.0	37.0	37.0	37.0	37.0
30-34	36.297399999999996	37.0	37.0	37.0	37.0	37.0
35-39	36.1985	37.0	37.0	37.0	37.0	37.0
40-44	36.2013	37.0	37.0	37.0	37.0	37.0
45-49	36.1503	37.0	37.0	37.0	37.0	37.0
50-54	36.121500000000005	37.0	37.0	37.0	37.0	37.0
55-59	36.15560000000001	37.0	37.0	37.0	37.0	37.0
60-64	36.11579999999999	37.0	37.0	37.0	37.0	37.0
65-69	36.0797	37.0	37.0	37.0	37.0	37.0
70-74	36.0202	37.0	37.0	37.0	37.0	37.0
75-79	35.9477	37.0	37.0	37.0	37.0	37.0
80-84	36.033300000000004	37.0	37.0	37.0	37.0	37.0
85-89	35.9637	37.0	37.0	37.0	37.0	37.0
90-94	35.9113	37.0	37.0	37.0	37.0	37.0
95-99	35.7822	37.0	37.0	37.0	37.0	37.0
100-104	35.752300000000005	37.0	37.0	37.0	37.0	37.0
105-109	35.7611	37.0	37.0	37.0	37.0	37.0
110-114	35.7447	37.0	37.0	37.0	37.0	37.0
115-119	35.643299999999996	37.0	37.0	37.0	37.0	37.0
120-124	35.564	37.0	37.0	37.0	37.0	37.0
125-129	35.496	37.0	37.0	37.0	37.0	37.0
130-134	35.497899999999994	37.0	37.0	37.0	37.0	37.0
135-139	35.35809999999999	37.0	37.0	37.0	34.6	37.0
140-144	35.517900000000004	37.0	37.0	37.0	37.0	37.0
145-149	35.2564	37.0	37.0	37.0	29.8	37.0
150	35.1065	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	0.0
23	0.0
24	3.0
25	5.0
26	5.0
27	10.0
28	21.0
29	34.0
30	36.0
31	43.0
32	67.0
33	92.0
34	198.0
35	552.0
36	2761.0
37	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.05	15.024999999999999	19.375	34.55
2	24.425	23.400000000000002	31.225	20.95
3	25.525	27.474999999999998	25.324999999999996	21.675
4	26.424999999999997	31.5	20.825	21.25
5	24.925	37.8	19.400000000000002	17.875
6	18.224999999999998	38.175	23.400000000000002	20.200000000000003
7	18.525	17.65	41.425	22.400000000000002
8	20.125	23.125	28.999999999999996	27.750000000000004
9	21.975	23.65	27.05	27.325
10-14	23.419999999999998	27.495000000000005	25.655	23.43
15-19	22.869999999999997	25.380000000000003	27.805000000000003	23.945
20-24	22.28	27.51	27.115000000000002	23.095
25-29	22.2	26.229999999999997	26.205000000000002	25.365
30-34	22.33	27.12	26.400000000000002	24.15
35-39	23.86	26.685	26.545	22.91
40-44	23.200000000000003	25.97	26.875	23.955000000000002
45-49	23.32	28.125	25.590000000000003	22.965
50-54	22.915	27.62	27.065	22.400000000000002
55-59	22.605	26.35	27.915	23.13
60-64	22.78	27.169999999999998	26.490000000000002	23.56
65-69	22.075	26.765	27.900000000000002	23.26
70-74	21.525	27.265	28.52	22.689999999999998
75-79	22.605	26.99	26.85	23.555
80-84	22.205	27.22	28.46	22.115000000000002
85-89	22.650000000000002	29.145	26.935	21.27
90-94	23.755000000000003	28.77	25.215	22.259999999999998
95-99	21.915000000000003	29.75	27.685	20.65
100-104	21.735	29.099999999999998	26.584999999999997	22.58
105-109	22.975	27.175	27.060000000000002	22.79
110-114	21.795	27.944999999999997	27.815	22.445
115-119	22.3	25.865	29.225	22.61
120-124	22.67	26.77	28.01	22.55
125-129	22.25	27.495000000000005	28.52	21.735
130-134	22.0	27.405	28.115000000000002	22.48
135-139	23.075000000000003	26.295	29.099999999999998	21.529999999999998
140-144	21.925	27.68	26.875	23.52
145-149	22.595000000000002	28.715000000000003	25.955000000000002	22.735
150	21.05	29.75	28.449999999999996	20.75
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	0.5
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	2.0
21	0.0
22	0.5
23	1.0
24	4.0
25	4.0
26	5.5
27	15.0
28	46.5
29	48.5
30	23.5
31	23.0
32	22.5
33	19.0
34	21.0
35	19.5
36	32.0
37	56.5
38	86.0
39	150.5
40	199.0
41	227.0
42	215.0
43	202.5
44	231.5
45	214.0
46	247.5
47	292.0
48	270.5
49	213.5
50	140.5
51	126.0
52	131.5
53	139.5
54	116.0
55	63.5
56	52.5
57	40.5
58	21.0
59	14.5
60	9.5
61	7.5
62	10.5
63	15.5
64	22.5
65	33.0
66	28.5
67	33.5
68	26.5
69	9.5
70	15.0
71	16.0
72	12.5
73	11.0
74	4.5
75	0.5
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.13418530351437	18.05
2	19.936102236421725	15.6
3	12.20447284345048	14.325
4	7.731629392971246	12.1
5	5.23961661341853	10.25
6	2.939297124600639	6.9
7	1.7252396166134185	4.725
8	0.8945686900958467	2.8000000000000003
9	0.5111821086261981	1.7999999999999998
>10	2.68370607028754	13.450000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	34	0.8500000000000001	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	21	0.525	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	18	0.44999999999999996	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	17	0.42500000000000004	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	16	0.4	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	16	0.4	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	15	0.375	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	15	0.375	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	14	0.35000000000000003	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	14	0.35000000000000003	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	13	0.325	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	13	0.325	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	13	0.325	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	13	0.325	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	13	0.325	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	13	0.325	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	12	0.3	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	12	0.3	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	12	0.3	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	12	0.3	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	12	0.3	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	12	0.3	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	12	0.3	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	12	0.3	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	11	0.27499999999999997	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	11	0.27499999999999997	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	11	0.27499999999999997	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	11	0.27499999999999997	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	10	0.25	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	10	0.25	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	10	0.25	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	10	0.25	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	10	0.25	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	10	0.25	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	10	0.25	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	10	0.25	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	10	0.25	No Hit
GTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGG	10	0.25	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	10	0.25	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	10	0.25	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	10	0.25	No Hit
GCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGT	10	0.25	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	9	0.22499999999999998	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	9	0.22499999999999998	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	9	0.22499999999999998	No Hit
GTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCA	9	0.22499999999999998	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	9	0.22499999999999998	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	9	0.22499999999999998	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	9	0.22499999999999998	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	9	0.22499999999999998	No Hit
CTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAAT	8	0.2	No Hit
CGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACT	8	0.2	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	8	0.2	No Hit
GCACTGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCA	8	0.2	No Hit
ATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTT	8	0.2	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	8	0.2	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	8	0.2	No Hit
GTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGG	8	0.2	No Hit
CTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGA	8	0.2	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	8	0.2	No Hit
AGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAAT	8	0.2	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	8	0.2	No Hit
GCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGT	8	0.2	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	8	0.2	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	7	0.17500000000000002	No Hit
GAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATT	7	0.17500000000000002	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	7	0.17500000000000002	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	7	0.17500000000000002	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	7	0.17500000000000002	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	7	0.17500000000000002	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	7	0.17500000000000002	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	7	0.17500000000000002	No Hit
GGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCG	7	0.17500000000000002	No Hit
TAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAA	7	0.17500000000000002	No Hit
CCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCT	7	0.17500000000000002	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	7	0.17500000000000002	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	7	0.17500000000000002	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	7	0.17500000000000002	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	7	0.17500000000000002	No Hit
GCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCG	7	0.17500000000000002	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	7	0.17500000000000002	No Hit
GCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTT	7	0.17500000000000002	No Hit
TATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGC	7	0.17500000000000002	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	7	0.17500000000000002	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	7	0.17500000000000002	No Hit
AAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGC	7	0.17500000000000002	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	7	0.17500000000000002	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	7	0.17500000000000002	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	7	0.17500000000000002	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	6	0.15	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	6	0.15	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	6	0.15	No Hit
AATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATT	6	0.15	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	6	0.15	No Hit
GCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATA	6	0.15	No Hit
AGACACTTAAAAGGATCATATATTTCCCATGAGGGTAACATCATTTAGTC	6	0.15	No Hit
TTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGC	6	0.15	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	6	0.15	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	6	0.15	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	6	0.15	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	6	0.15	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	6	0.15	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	6	0.15	No Hit
TGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCAT	6	0.15	No Hit
GCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCA	6	0.15	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	6	0.15	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	6	0.15	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	6	0.15	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	6	0.15	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	6	0.15	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	6	0.15	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	6	0.15	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	6	0.15	No Hit
TTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCA	6	0.15	No Hit
GAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTA	6	0.15	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	6	0.15	No Hit
TGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAA	6	0.15	No Hit
CTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAA	6	0.15	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	6	0.15	No Hit
AAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATT	6	0.15	No Hit
CCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGA	6	0.15	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	6	0.15	No Hit
CTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGC	6	0.15	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	6	0.15	No Hit
TGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATC	6	0.15	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	6	0.15	No Hit
TGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCG	6	0.15	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	6	0.15	No Hit
TGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTT	6	0.15	No Hit
TTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATT	6	0.15	No Hit
TGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACT	6	0.15	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	6	0.15	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	6	0.15	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	6	0.15	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	6	0.15	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	5	0.125	No Hit
GCGCCTTCCATGGAATTCACGTGGTCCACTCTAGCCCTTGTGCCAGTCAC	5	0.125	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	5	0.125	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	5	0.125	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	5	0.125	No Hit
CTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGT	5	0.125	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	5	0.125	No Hit
TGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTA	5	0.125	No Hit
TTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAAT	5	0.125	No Hit
TCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTC	5	0.125	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	5	0.125	No Hit
CACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCA	5	0.125	No Hit
CAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGG	5	0.125	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
TTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATT	5	0.125	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	5	0.125	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	5	0.125	No Hit
CGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGG	5	0.125	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	5	0.125	No Hit
GGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGC	5	0.125	No Hit
CCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGAT	5	0.125	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	5	0.125	No Hit
CATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGC	5	0.125	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	5	0.125	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	5	0.125	No Hit
CTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGT	5	0.125	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	5	0.125	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	5	0.125	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	5	0.125	No Hit
TTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAATGT	5	0.125	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	5	0.125	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	5	0.125	No Hit
TATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTT	5	0.125	No Hit
CGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCA	5	0.125	No Hit
TTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAGCAGAA	5	0.125	No Hit
GGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATC	5	0.125	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	5	0.125	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	5	0.125	No Hit
GGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCT	5	0.125	No Hit
GGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCA	5	0.125	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	5	0.125	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	5	0.125	No Hit
GAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGC	5	0.125	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	5	0.125	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	5	0.125	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	5	0.125	No Hit
AATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGT	5	0.125	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	5	0.125	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	5	0.125	No Hit
CAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATC	5	0.125	No Hit
ATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAA	5	0.125	No Hit
CCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAA	5	0.125	No Hit
CAAGGCTTTTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCT	5	0.125	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	5	0.125	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	5	0.125	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	5	0.125	No Hit
CTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCC	5	0.125	No Hit
CCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAA	5	0.125	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	5	0.125	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	5	0.125	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	5	0.125	No Hit
GGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATACTGT	5	0.125	No Hit
AATTGATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCAC	5	0.125	No Hit
TGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCG	5	0.125	No Hit
AGAAATTAAAACAGTGACTCTACTCACTGCTTCCAGAGACACCTACCTGC	5	0.125	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	5	0.125	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	5	0.125	No Hit
GCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGG	5	0.125	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	5	0.125	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	5	0.125	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	5	0.125	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	5	0.125	No Hit
TTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAA	5	0.125	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	5	0.125	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	5	0.125	No Hit
TTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCT	5	0.125	No Hit
GGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAG	5	0.125	No Hit
CTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAA	5	0.125	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	5	0.125	No Hit
TGGACAGTGATTGTGGGGAGCAAGTCCCTCAAGGCATTTAAAACAAAAAT	5	0.125	No Hit
CCTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
Read 5719562 spots for SRR25558761.sra
Written 5719562 spots for SRR25558761.sra
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
Read 5719556 spots for SRR25558761.sra
Written 5719556 spots for SRR25558761.sra
SRR ids: ['SRR25558761.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qcr9mgur
SRR25558761.sra spots: 45756454
blocks: [[1, 5719556], [5719557, 11439112], [11439113, 17158668], [17158669, 22878224], [22878225, 28597780], [28597781, 34317336], [34317337, 40036892], [40036893, 45756454]]
SRR25558761 file size 15438992
SRR25558761 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25558761 SRR25558761_1.fastq SRR25558761_2.fastq
Input file:	SRR25558761_1.fastq
Paired file:	SRR25558761_2.fastq
trimmed:	SRR25558761-trimmed-pair1.fastq, SRR25558761-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun May 24 15:29:48 2026 >> started

Sun May 24 15:31:10 2026 >> done (82.193s)
45756454 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
      37 ( 0.00%) empty read pairs filtered out after trimming by size control
45756417 (100.00%) read pairs available; of these:
  343028 ( 0.75%) trimmed read pairs available after processing
45413389 (99.25%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 22	       1	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       1	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       1	  0.00%
 55	       0	  0.00%
 56	       1	  0.00%
 57	       0	  0.00%
 58	       1	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       1	  0.00%
 63	       1	  0.00%
 64	       3	  0.00%
 65	       3	  0.00%
 66	       1	  0.00%
 67	       0	  0.00%
 68	       8	  0.00%
 69	       3	  0.00%
 70	       2	  0.00%
 71	       4	  0.00%
 72	       4	  0.00%
 73	       6	  0.00%
 74	       4	  0.00%
 75	       9	  0.00%
 76	       4	  0.00%
 77	       8	  0.00%
 78	      10	  0.00%
 79	       7	  0.00%
 80	      10	  0.00%
 81	       9	  0.00%
 82	      11	  0.00%
 83	       8	  0.00%
 84	       9	  0.00%
 85	      10	  0.00%
 86	       9	  0.00%
 87	       9	  0.00%
 88	       8	  0.00%
 89	      11	  0.00%
 90	      14	  0.00%
 91	      17	  0.00%
 92	      11	  0.00%
 93	      17	  0.00%
 94	      25	  0.00%
 95	      22	  0.00%
 96	      21	  0.00%
 97	      21	  0.00%
 98	      30	  0.00%
 99	      29	  0.00%
100	      32	  0.00%
101	      41	  0.00%
102	      49	  0.00%
103	      44	  0.00%
104	      51	  0.00%
105	      59	  0.00%
106	      51	  0.00%
107	      53	  0.00%
108	      64	  0.00%
109	      72	  0.00%
110	      74	  0.00%
111	      67	  0.00%
112	      93	  0.00%
113	      83	  0.00%
114	     120	  0.00%
115	     115	  0.00%
116	     132	  0.00%
117	     126	  0.00%
118	     120	  0.00%
119	     133	  0.00%
120	     173	  0.00%
121	     174	  0.00%
122	     202	  0.00%
123	     182	  0.00%
124	     213	  0.00%
125	     241	  0.00%
126	     256	  0.00%
127	     247	  0.00%
128	     281	  0.00%
129	     307	  0.00%
130	     309	  0.00%
131	     337	  0.00%
132	     356	  0.00%
133	     373	  0.00%
134	     399	  0.00%
135	     457	  0.00%
136	     464	  0.00%
137	     472	  0.00%
138	     530	  0.00%
139	     585	  0.00%
140	     583	  0.00%
141	   30482	  0.07%
142	   31697	  0.07%
143	   32528	  0.07%
144	   34813	  0.08%
145	   36536	  0.08%
146	   38796	  0.08%
147	   40783	  0.09%
148	   43278	  0.09%
149	   45011	  0.10%
150	45413389	 99.25%
45756417 reads passed initial QC


criterion=sequence-density
sequence-density=1.32
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=36
prefix-density=1.33
prefix-fanout=2.0
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=73.23
fanout-score-rank=1
prefix-density=0.23
prefix-fanout=2.2
sequence=TTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTA


criterion=sequence-density
sequence-density=1.35
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=39
prefix-density=1.35
prefix-fanout=2.0
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=31
fanout-score=75.90
fanout-score-rank=1
prefix-density=1.73
prefix-fanout=1.2
sequence=CATCATGATGCTTGTGTATTGTTATTTTTTTTTGGCAATGATTCTCACTTATATAGTCAACCTAGAAATAGCATTTTTTCTTCCCATGATTCACAAAGTGAGATTGGATTTCTTTGATAGATCCTTGCTTCAGGTTTTAGAAGATGTCAGCAGATGACAGAGGACACAGAAGTGGTTATTTTCTTGATATTCATGTTGGATTTTAATATAAGTCAAGTGAAATGTATAGGCTGATTCACCTTCTGCTCCAATTTGGAAACCCTGTAGAAGTTCCTGTTCCTTTTGTAGGATGGATTCTGGCAGTAGCCTGACTCTCACCTTGCATTCTGTATACCACACATATTACACATAGGGCCTCCATTCCTTTACATGCAACAT
SRR25558761 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 15:31:29
                             Started mapping on |	May 24 15:31:29
                                    Finished on |	May 24 15:38:48
       Mapping speed, Million of reads per hour |	375.22

                          Number of input reads |	45756417
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	40343604
                        Uniquely mapped reads % |	88.17%
                          Average mapped length |	298.82
                       Number of splices: Total |	46140126
            Number of splices: Annotated (sjdb) |	45862555
                       Number of splices: GT/AG |	45812083
                       Number of splices: GC/AG |	276235
                       Number of splices: AT/AC |	19420
               Number of splices: Non-canonical |	32388
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.69
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.31
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2410271
             % of reads mapped to multiple loci |	5.27%
        Number of reads mapped to too many loci |	37794
             % of reads mapped to too many loci |	0.08%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.37%
                     % of reads unmapped: other |	5.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3002542	3002542	3002542
N_multimapping	2410271	2410271	2410271
N_noFeature	1366160	20050414	20126550
N_ambiguous	2931519	760108	752897
UnstrandedReadsAssigned:36045925 PositiveStrandReadsAssigned:19533082 NegativeStrandReadsAssigned:19464157
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25558761 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558761-trimmed-pair1.fastq
                             SRR25558761-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 45,756,417 reads, 43,285,721 reads pseudoaligned
[quant] estimated average fragment length: 234.44
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,134 rounds

 109283 SRR25558761.ke.tsv
  52636 SRR25558761.se.tsv
 161919 total
==> SRR25558761.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	16	0	0
ENSMUST00000196221.1	9	9	0	0
ENSMUST00000179664.1	11	11	0	0
ENSMUST00000178537.1	12	12	0	0
ENSMUST00000178862.1	14	14	0	0
ENSMUST00000179520.1	11	11	0	0
ENSMUST00000179883.1	16	16	0	0
ENSMUST00000195858.1	10	10	0	0
ENSMUST00000179932.1	12	12	0	0

==> SRR25558761.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558761 completed mapping pipeline successfully
