Starting /dee2/code/volunteer_pipeline.sh SRR25558758
    current disk space = 460508267520
    free memory = 366485128 
SRR25558758 SRAfilesize
ca63480938606dc788fca48d5f2638f0  SRR25558758.sra
SRR25558758.sra file validated
SRR25558758 is paired end
SRR25558758 is conventional basespace
SRR25558758 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558758_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.565	37.0	37.0	37.0	37.0	37.0
2	36.521	37.0	37.0	37.0	37.0	37.0
3	36.647	37.0	37.0	37.0	37.0	37.0
4	36.536	37.0	37.0	37.0	37.0	37.0
5	36.6455	37.0	37.0	37.0	37.0	37.0
6	36.5065	37.0	37.0	37.0	37.0	37.0
7	36.62	37.0	37.0	37.0	37.0	37.0
8	36.5965	37.0	37.0	37.0	37.0	37.0
9	36.6055	37.0	37.0	37.0	37.0	37.0
10-14	36.6102	37.0	37.0	37.0	37.0	37.0
15-19	36.5065	37.0	37.0	37.0	37.0	37.0
20-24	36.552800000000005	37.0	37.0	37.0	37.0	37.0
25-29	36.4958	37.0	37.0	37.0	37.0	37.0
30-34	36.49415	37.0	37.0	37.0	37.0	37.0
35-39	36.466899999999995	37.0	37.0	37.0	37.0	37.0
40-44	36.448699999999995	37.0	37.0	37.0	37.0	37.0
45-49	36.443	37.0	37.0	37.0	37.0	37.0
50-54	36.396	37.0	37.0	37.0	37.0	37.0
55-59	36.38295	37.0	37.0	37.0	37.0	37.0
60-64	36.386700000000005	37.0	37.0	37.0	37.0	37.0
65-69	36.3686	37.0	37.0	37.0	37.0	37.0
70-74	36.3358	37.0	37.0	37.0	37.0	37.0
75-79	36.3575	37.0	37.0	37.0	37.0	37.0
80-84	36.27755	37.0	37.0	37.0	37.0	37.0
85-89	36.2966	37.0	37.0	37.0	37.0	37.0
90-94	36.2666	37.0	37.0	37.0	37.0	37.0
95-99	36.261399999999995	37.0	37.0	37.0	37.0	37.0
100-104	36.2659	37.0	37.0	37.0	37.0	37.0
105-109	36.1457	37.0	37.0	37.0	37.0	37.0
110-114	36.1649	37.0	37.0	37.0	37.0	37.0
115-119	36.1114	37.0	37.0	37.0	37.0	37.0
120-124	36.073699999999995	37.0	37.0	37.0	37.0	37.0
125-129	36.0315	37.0	37.0	37.0	37.0	37.0
130-134	35.9736	37.0	37.0	37.0	37.0	37.0
135-139	35.93240000000001	37.0	37.0	37.0	37.0	37.0
140-144	35.901399999999995	37.0	37.0	37.0	37.0	37.0
145-149	35.88719999999999	37.0	37.0	37.0	37.0	37.0
150	35.803	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	2.0
24	0.0
25	1.0
26	3.0
27	4.0
28	8.0
29	14.0
30	30.0
31	30.0
32	38.0
33	89.0
34	99.0
35	316.0
36	2995.0
37	369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.8	15.775	16.625	36.8
2	22.670340681362724	25.275551102204407	34.81963927855711	17.234468937875754
3	26.200000000000003	27.125	24.675	22.0
4	28.000000000000004	33.575	19.925	18.5
5	23.200000000000003	35.775	21.875	19.15
6	17.8	38.35	23.625	20.225
7	19.2	16.0	41.875	22.925
8	19.125	22.075	32.4	26.400000000000002
9	21.725	23.65	28.65	25.974999999999998
10-14	22.74	28.815	24.740000000000002	23.705000000000002
15-19	22.88	25.650000000000002	27.71	23.76
20-24	21.325	27.400000000000002	28.27	23.005
25-29	23.1	27.205000000000002	25.295	24.4
30-34	22.096104805240262	27.281364068203413	27.226361318065905	23.396169808490423
35-39	23.555	26.44	26.400000000000002	23.605
40-44	23.605	26.055	26.705000000000002	23.635
45-49	22.67	27.075	25.795	24.46
50-54	22.759999999999998	27.915	26.834999999999997	22.49
55-59	21.69108455422771	26.996349817490874	27.826391319565978	23.486174308715434
60-64	24.104999999999997	27.255000000000003	26.685	21.955
65-69	22.18	27.529999999999998	27.825	22.465
70-74	22.275	27.255000000000003	27.46	23.01
75-79	23.119999999999997	27.245	27.38	22.255
80-84	22.78113905695285	27.33136656832842	28.521426071303562	21.36606830341517
85-89	23.435	27.58	27.18	21.805
90-94	23.799999999999997	28.275	25.635	22.29
95-99	22.775000000000002	28.34	27.38	21.505
100-104	21.205	28.685	27.445000000000004	22.665
105-109	22.705000000000002	26.99	27.61	22.695
110-114	22.175	27.034999999999997	27.58	23.21
115-119	22.06	26.33	29.34	22.27
120-124	22.35	25.895000000000003	29.509999999999998	22.245
125-129	23.200000000000003	27.74	28.28	20.78
130-134	21.465	27.505000000000003	28.970000000000002	22.06
135-139	23.369999999999997	25.71	28.349999999999998	22.57
140-144	22.74	27.834999999999997	26.935	22.49
145-149	22.009999999999998	28.65	26.095000000000002	23.244999999999997
150	22.775000000000002	30.5	26.25	20.474999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.5
18	1.5
19	2.0
20	1.0
21	0.5
22	1.0
23	1.0
24	2.5
25	6.0
26	8.5
27	9.0
28	20.0
29	27.0
30	30.5
31	27.0
32	13.0
33	10.5
34	17.5
35	21.0
36	34.0
37	68.0
38	92.0
39	148.0
40	212.0
41	242.0
42	248.0
43	237.5
44	233.5
45	207.0
46	249.0
47	276.5
48	262.0
49	213.5
50	136.0
51	117.0
52	118.5
53	134.5
54	117.5
55	78.5
56	56.5
57	39.5
58	20.5
59	15.5
60	14.0
61	11.0
62	8.0
63	11.5
64	23.0
65	34.0
66	31.5
67	26.0
68	19.5
69	8.0
70	12.0
71	18.0
72	10.5
73	5.5
74	2.5
75	0.5
76	2.0
77	1.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.2
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.005
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.005
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.005
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.32309442548351	23.875
2	20.477815699658702	18.0
3	9.783845278725826	12.9
4	4.778156996587031	8.4
5	3.35608646188851	7.375
6	2.04778156996587	5.4
7	1.4789533560864618	4.55
8	0.5688282138794084	2.0
9	0.9670079635949944	3.8249999999999997
>10	2.218430034129693	13.675
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	22	0.5499999999999999	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	22	0.5499999999999999	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	22	0.5499999999999999	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	21	0.525	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	21	0.525	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	20	0.5	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	19	0.475	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	18	0.44999999999999996	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	17	0.42500000000000004	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	17	0.42500000000000004	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	15	0.375	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	15	0.375	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	14	0.35000000000000003	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	14	0.35000000000000003	No Hit
CTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCA	14	0.35000000000000003	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	14	0.35000000000000003	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	13	0.325	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	13	0.325	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	13	0.325	No Hit
GCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAAT	12	0.3	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	12	0.3	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	12	0.3	No Hit
GTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGA	12	0.3	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	12	0.3	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	12	0.3	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	12	0.3	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	12	0.3	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	12	0.3	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	11	0.27499999999999997	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	11	0.27499999999999997	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	11	0.27499999999999997	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	11	0.27499999999999997	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	11	0.27499999999999997	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	10	0.25	No Hit
TGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCAG	10	0.25	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	10	0.25	No Hit
CTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCG	10	0.25	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	10	0.25	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	10	0.25	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	9	0.22499999999999998	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	9	0.22499999999999998	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	9	0.22499999999999998	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	9	0.22499999999999998	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	9	0.22499999999999998	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	9	0.22499999999999998	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	9	0.22499999999999998	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	9	0.22499999999999998	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCA	9	0.22499999999999998	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	9	0.22499999999999998	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	9	0.22499999999999998	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	9	0.22499999999999998	No Hit
GTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTG	9	0.22499999999999998	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	9	0.22499999999999998	No Hit
GAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAG	9	0.22499999999999998	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	9	0.22499999999999998	No Hit
CACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGA	9	0.22499999999999998	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	8	0.2	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	8	0.2	No Hit
CTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATA	8	0.2	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	8	0.2	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	8	0.2	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	8	0.2	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	8	0.2	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	8	0.2	No Hit
TTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCT	8	0.2	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	8	0.2	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	7	0.17500000000000002	No Hit
GCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATA	7	0.17500000000000002	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	7	0.17500000000000002	No Hit
TGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTA	7	0.17500000000000002	No Hit
CCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAG	7	0.17500000000000002	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	7	0.17500000000000002	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	7	0.17500000000000002	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	7	0.17500000000000002	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	7	0.17500000000000002	No Hit
GGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGG	7	0.17500000000000002	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	7	0.17500000000000002	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	7	0.17500000000000002	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	7	0.17500000000000002	No Hit
AACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCA	7	0.17500000000000002	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	7	0.17500000000000002	No Hit
TGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAAT	7	0.17500000000000002	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	7	0.17500000000000002	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	7	0.17500000000000002	No Hit
GCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCA	7	0.17500000000000002	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	7	0.17500000000000002	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	7	0.17500000000000002	No Hit
ATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATAC	7	0.17500000000000002	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	7	0.17500000000000002	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	7	0.17500000000000002	No Hit
TTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAATA	6	0.15	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	6	0.15	No Hit
CAGAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCT	6	0.15	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	6	0.15	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	6	0.15	No Hit
TGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACA	6	0.15	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	6	0.15	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	6	0.15	No Hit
GCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGC	6	0.15	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	6	0.15	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	6	0.15	No Hit
AAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAA	6	0.15	No Hit
GCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAG	6	0.15	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	6	0.15	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	6	0.15	No Hit
CAGTAACTAAAAACTGTGGAAGGTGAATCTGAAGGACTGCTTACTCAACA	6	0.15	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	6	0.15	No Hit
TTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGCA	6	0.15	No Hit
GGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCC	6	0.15	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	6	0.15	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	6	0.15	No Hit
CAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGG	6	0.15	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	6	0.15	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	6	0.15	No Hit
TGCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCC	6	0.15	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	6	0.15	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	6	0.15	No Hit
GTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGG	6	0.15	No Hit
CCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCT	6	0.15	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	6	0.15	No Hit
ATGCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGC	6	0.15	No Hit
CTTCCTGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATA	6	0.15	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	6	0.15	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	6	0.15	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	5	0.125	No Hit
GCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGGTCAGTCATGG	5	0.125	No Hit
GGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGG	5	0.125	No Hit
TTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCT	5	0.125	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	5	0.125	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	5	0.125	No Hit
CGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACTGGC	5	0.125	No Hit
CTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCAT	5	0.125	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	5	0.125	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	5	0.125	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	5	0.125	No Hit
CGGAAATATCTCGATTAGCACTGTTGATCGGCCCCTGTGAAAACGAAGCC	5	0.125	No Hit
GGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAG	5	0.125	No Hit
TGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGAT	5	0.125	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	5	0.125	No Hit
CTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCC	5	0.125	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	5	0.125	No Hit
TGTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACAT	5	0.125	No Hit
ATTATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGC	5	0.125	No Hit
GGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGA	5	0.125	No Hit
ATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTG	5	0.125	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	5	0.125	No Hit
ATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGA	5	0.125	No Hit
ATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGG	5	0.125	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	5	0.125	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	5	0.125	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	5	0.125	No Hit
TGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCG	5	0.125	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	5	0.125	No Hit
GTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGT	5	0.125	No Hit
GAGAAATGTCCAAATTCTGGAATCAGTTGTAACAATAAAACTCAATAGAA	5	0.125	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	5	0.125	No Hit
AAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGA	5	0.125	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	5	0.125	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	5	0.125	No Hit
TGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATAGCCAT	5	0.125	No Hit
GGATCATATATTTCCCATGAGGGTAACATCATTTAGTCTGTATCATTGGT	5	0.125	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	5	0.125	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	5	0.125	No Hit
ATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGG	5	0.125	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	5	0.125	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	5	0.125	No Hit
GTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCG	5	0.125	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	5	0.125	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	5	0.125	No Hit
GTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCG	5	0.125	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	5	0.125	No Hit
GAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATA	5	0.125	No Hit
CAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAAG	5	0.125	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	5	0.125	No Hit
TGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGA	5	0.125	No Hit
TGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCA	5	0.125	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	5	0.125	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	5	0.125	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	5	0.125	No Hit
CCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAG	5	0.125	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	5	0.125	No Hit
CTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTA	5	0.125	No Hit
GAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25558758 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25558758_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.475	37.0	37.0	37.0	37.0	37.0
2	36.42125	37.0	37.0	37.0	37.0	37.0
3	36.554	37.0	37.0	37.0	37.0	37.0
4	36.5015	37.0	37.0	37.0	37.0	37.0
5	36.415	37.0	37.0	37.0	37.0	37.0
6	36.4835	37.0	37.0	37.0	37.0	37.0
7	36.4785	37.0	37.0	37.0	37.0	37.0
8	36.463	37.0	37.0	37.0	37.0	37.0
9	36.501	37.0	37.0	37.0	37.0	37.0
10-14	36.4519	37.0	37.0	37.0	37.0	37.0
15-19	36.4547	37.0	37.0	37.0	37.0	37.0
20-24	36.4563	37.0	37.0	37.0	37.0	37.0
25-29	36.32615	37.0	37.0	37.0	37.0	37.0
30-34	36.3831	37.0	37.0	37.0	37.0	37.0
35-39	36.3436	37.0	37.0	37.0	37.0	37.0
40-44	36.3418	37.0	37.0	37.0	37.0	37.0
45-49	36.3037	37.0	37.0	37.0	37.0	37.0
50-54	36.3055	37.0	37.0	37.0	37.0	37.0
55-59	36.2024	37.0	37.0	37.0	37.0	37.0
60-64	36.2234	37.0	37.0	37.0	37.0	37.0
65-69	36.217200000000005	37.0	37.0	37.0	37.0	37.0
70-74	36.0871	37.0	37.0	37.0	37.0	37.0
75-79	35.99820000000001	37.0	37.0	37.0	37.0	37.0
80-84	36.01700000000001	37.0	37.0	37.0	37.0	37.0
85-89	35.9962	37.0	37.0	37.0	37.0	37.0
90-94	36.020500000000006	37.0	37.0	37.0	37.0	37.0
95-99	35.8754	37.0	37.0	37.0	37.0	37.0
100-104	35.83389999999999	37.0	37.0	37.0	37.0	37.0
105-109	35.8896	37.0	37.0	37.0	37.0	37.0
110-114	35.84409999999999	37.0	37.0	37.0	37.0	37.0
115-119	35.646300000000004	37.0	37.0	37.0	37.0	37.0
120-124	35.6548	37.0	37.0	37.0	37.0	37.0
125-129	35.5927	37.0	37.0	37.0	37.0	37.0
130-134	35.634	37.0	37.0	37.0	37.0	37.0
135-139	35.581	37.0	37.0	37.0	37.0	37.0
140-144	35.4392	37.0	37.0	37.0	34.6	37.0
145-149	35.27239999999999	37.0	37.0	37.0	27.4	37.0
150	35.138	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	0.0
23	0.0
24	3.0
25	3.0
26	5.0
27	6.0
28	7.0
29	19.0
30	25.0
31	32.0
32	61.0
33	97.0
34	163.0
35	585.0
36	2823.0
37	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.65	16.175	17.525	36.65
2	22.53063265816454	25.331332833208304	32.85821455363841	19.279819954988746
3	26.375	26.974999999999998	25.25	21.4
4	27.250000000000004	33.300000000000004	20.25	19.2
5	23.35	36.475	22.025	18.15
6	20.075000000000003	36.675000000000004	23.525	19.725
7	18.475	16.650000000000002	42.475	22.400000000000002
8	19.825	23.5	30.425	26.25
9	22.375	24.775	26.85	26.0
10-14	23.45	28.625	24.48	23.445
15-19	22.759999999999998	25.465	27.73	24.044999999999998
20-24	21.845	27.43	27.6	23.125
25-29	22.616130806540326	26.64133206660333	26.281314065703288	24.46122306115306
30-34	21.834999999999997	28.115000000000002	26.36	23.69
35-39	23.68	27.33	25.89	23.1
40-44	23.35	26.265	26.11	24.275
45-49	21.834999999999997	27.994999999999997	26.22	23.95
50-54	22.825	27.839999999999996	27.134999999999998	22.2
55-59	21.765	26.634999999999998	27.915	23.685000000000002
60-64	24.14	27.345000000000002	26.400000000000002	22.115000000000002
65-69	22.555	26.765	27.644999999999996	23.035
70-74	22.1	26.784999999999997	27.744999999999997	23.369999999999997
75-79	23.724999999999998	27.82	26.115	22.34
80-84	22.595000000000002	27.634999999999998	28.375	21.395
85-89	22.939999999999998	27.875	27.705000000000002	21.48
90-94	23.56	27.894999999999996	25.89	22.655
95-99	21.875	28.465	28.04	21.62
100-104	21.78	28.634999999999998	26.740000000000002	22.845
105-109	22.41	27.865000000000002	27.02	22.705000000000002
110-114	21.4	27.025	28.515	23.06
115-119	22.12	27.450000000000003	27.83	22.6
120-124	22.770000000000003	27.1	28.360000000000003	21.77
125-129	21.88	28.255000000000003	28.42	21.445
130-134	21.375	27.994999999999997	28.384999999999998	22.245
135-139	22.18	27.11	28.395	22.314999999999998
140-144	22.264999999999997	28.09	27.310000000000002	22.335
145-149	22.37	29.015	25.41	23.205000000000002
150	23.400000000000002	30.349999999999998	24.65	21.6
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.5
12	0.5
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.0
20	1.0
21	2.5
22	2.5
23	1.5
24	3.5
25	5.0
26	4.5
27	6.0
28	15.0
29	26.0
30	28.0
31	16.5
32	16.0
33	24.5
34	23.0
35	27.0
36	46.5
37	76.5
38	92.0
39	146.0
40	224.5
41	254.0
42	252.5
43	233.5
44	217.0
45	185.5
46	244.5
47	304.0
48	277.0
49	189.0
50	103.5
51	115.5
52	124.5
53	132.0
54	113.5
55	75.5
56	54.5
57	42.0
58	29.0
59	19.0
60	13.5
61	10.0
62	7.0
63	5.0
64	17.0
65	29.5
66	31.0
67	32.5
68	23.5
69	12.0
70	18.5
71	18.5
72	9.5
73	5.0
74	3.5
75	2.5
76	0.5
77	1.0
78	2.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.706533776301214	24.7
2	21.483942414174972	19.400000000000002
3	8.693244739756366	11.774999999999999
4	5.149501661129568	9.3
5	2.823920265780731	6.375
6	2.602436323366556	7.049999999999999
7	1.4396456256921373	4.55
8	0.9413067552602437	3.4000000000000004
9	0.4429678848283499	1.7999999999999998
>10	1.716500553709856	11.65
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	30	0.75	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	28	0.7000000000000001	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	26	0.65	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	21	0.525	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	21	0.525	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	18	0.44999999999999996	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	17	0.42500000000000004	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	17	0.42500000000000004	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	16	0.4	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	16	0.4	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	15	0.375	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	15	0.375	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	15	0.375	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	14	0.35000000000000003	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	14	0.35000000000000003	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	13	0.325	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	13	0.325	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	13	0.325	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	13	0.325	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	12	0.3	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	12	0.3	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	12	0.3	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	11	0.27499999999999997	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	11	0.27499999999999997	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	11	0.27499999999999997	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	11	0.27499999999999997	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	11	0.27499999999999997	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	10	0.25	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	10	0.25	No Hit
TGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATAT	10	0.25	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	10	0.25	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	9	0.22499999999999998	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	9	0.22499999999999998	No Hit
TGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAG	9	0.22499999999999998	No Hit
CTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTGG	9	0.22499999999999998	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	9	0.22499999999999998	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	9	0.22499999999999998	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	9	0.22499999999999998	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	9	0.22499999999999998	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	8	0.2	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	8	0.2	No Hit
CTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAA	8	0.2	No Hit
CAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCAT	8	0.2	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	8	0.2	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	8	0.2	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	8	0.2	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	8	0.2	No Hit
CGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTG	8	0.2	No Hit
GCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATG	8	0.2	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	8	0.2	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	8	0.2	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	8	0.2	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	8	0.2	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	8	0.2	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	8	0.2	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	8	0.2	No Hit
CAGAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCT	7	0.17500000000000002	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	7	0.17500000000000002	No Hit
TAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAG	7	0.17500000000000002	No Hit
CTTCGGGCTTCGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATAT	7	0.17500000000000002	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	7	0.17500000000000002	No Hit
GTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGA	7	0.17500000000000002	No Hit
CTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTT	7	0.17500000000000002	No Hit
CTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTG	7	0.17500000000000002	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	7	0.17500000000000002	No Hit
CCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA	7	0.17500000000000002	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	7	0.17500000000000002	No Hit
TGTTGATCGGCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTG	7	0.17500000000000002	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	7	0.17500000000000002	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	7	0.17500000000000002	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	7	0.17500000000000002	No Hit
GATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGG	7	0.17500000000000002	No Hit
CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGC	7	0.17500000000000002	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	7	0.17500000000000002	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	7	0.17500000000000002	No Hit
GCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGG	7	0.17500000000000002	No Hit
TGGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCG	7	0.17500000000000002	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	7	0.17500000000000002	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	7	0.17500000000000002	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	7	0.17500000000000002	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	7	0.17500000000000002	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	7	0.17500000000000002	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	6	0.15	No Hit
GCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAAT	6	0.15	No Hit
AAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAAGA	6	0.15	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	6	0.15	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	6	0.15	No Hit
AAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCAT	6	0.15	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	6	0.15	No Hit
TATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCC	6	0.15	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	6	0.15	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	6	0.15	No Hit
CTCTAATGTCCTCTTTTGAACTCTGTGCGTACATATGGTGCACATAAACA	6	0.15	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	6	0.15	No Hit
GCCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	6	0.15	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	6	0.15	No Hit
GCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTG	6	0.15	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	6	0.15	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	6	0.15	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	6	0.15	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	6	0.15	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	6	0.15	No Hit
ATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGA	6	0.15	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	6	0.15	No Hit
AGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGCC	6	0.15	No Hit
GCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAAC	6	0.15	No Hit
GGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATC	6	0.15	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	6	0.15	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	6	0.15	No Hit
CAACGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGACT	6	0.15	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	6	0.15	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	6	0.15	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	6	0.15	No Hit
GAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCT	6	0.15	No Hit
GTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAAAGACTA	6	0.15	No Hit
CAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTG	6	0.15	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	6	0.15	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	6	0.15	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	6	0.15	No Hit
CAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGG	6	0.15	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	6	0.15	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	6	0.15	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	6	0.15	No Hit
TGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCT	6	0.15	No Hit
GTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCA	6	0.15	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	6	0.15	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	6	0.15	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	6	0.15	No Hit
CCAGTTTTGAGTACCATTGTGTGGGACTGTAGTTCCAGTTTTGAGTACCA	6	0.15	No Hit
CGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGA	5	0.125	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	5	0.125	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	5	0.125	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	5	0.125	No Hit
TGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTC	5	0.125	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	5	0.125	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	5	0.125	No Hit
CGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAAT	5	0.125	No Hit
TGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACA	5	0.125	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	5	0.125	No Hit
AGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCC	5	0.125	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
GGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAG	5	0.125	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	5	0.125	No Hit
CTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCG	5	0.125	No Hit
GCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTG	5	0.125	No Hit
TATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTT	5	0.125	No Hit
CTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCC	5	0.125	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	5	0.125	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	5	0.125	No Hit
GGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAG	5	0.125	No Hit
AAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGA	5	0.125	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	5	0.125	No Hit
ATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTAGGG	5	0.125	No Hit
CGGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAG	5	0.125	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	5	0.125	No Hit
TGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCC	5	0.125	No Hit
CCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCC	5	0.125	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	5	0.125	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	5	0.125	No Hit
TCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTT	5	0.125	No Hit
GCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCG	5	0.125	No Hit
CACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATAA	5	0.125	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	5	0.125	No Hit
AGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAG	5	0.125	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	5	0.125	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	5	0.125	No Hit
TGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCC	5	0.125	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	5	0.125	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	5	0.125	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	5	0.125	No Hit
ATGTAGGTACTGGGAATCGAACTTGGATTCTCTGGAAGAGCAAGTGCTCT	5	0.125	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	5	0.125	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	5	0.125	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	5	0.125	No Hit
ATCATTTCCATTTTGCTTACTTAAGCCCGTTTAACTACAAAGTTCTTGGA	5	0.125	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	5	0.125	No Hit
AGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCAC	5	0.125	No Hit
TGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAAT	5	0.125	No Hit
CATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGAAT	15	1.1730364E-4	144.0	1
ATCCATG	15	1.1730364E-4	144.0	6
AGAATCC	15	1.1730364E-4	144.0	3
AAGAATC	15	1.1730364E-4	144.0	2
GAATCCA	15	1.1730364E-4	144.0	4
TCCATGT	15	1.1730364E-4	144.0	7
CATGTGC	15	1.1730364E-4	144.0	9
CCATGTG	20	3.687869E-4	108.0	8
AATCCAT	35	0.0034045284	61.714283	5
>>END_MODULE
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597103 spots for SRR25558758.sra
Written 2597103 spots for SRR25558758.sra
Read 2597112 spots for SRR25558758.sra
Written 2597112 spots for SRR25558758.sra
SRR ids: ['SRR25558758.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7dt_1wxa
SRR25558758.sra spots: 41553657
blocks: [[1, 2597103], [2597104, 5194206], [5194207, 7791309], [7791310, 10388412], [10388413, 12985515], [12985516, 15582618], [15582619, 18179721], [18179722, 20776824], [20776825, 23373927], [23373928, 25971030], [25971031, 28568133], [28568134, 31165236], [31165237, 33762339], [33762340, 36359442], [36359443, 38956545], [38956546, 41553657]]
SRR25558758 file size 1835894
SRR25558758 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25558758 SRR25558758_1.fastq SRR25558758_2.fastq
Input file:	SRR25558758_1.fastq
Paired file:	SRR25558758_2.fastq
trimmed:	SRR25558758-trimmed-pair1.fastq, SRR25558758-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 16:04:33 2026 >> started

Sun May 24 16:06:18 2026 >> done (105.419s)
41553657 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
      22 ( 0.00%) empty read pairs filtered out after trimming by size control
41553635 (100.00%) read pairs available; of these:
  328825 ( 0.79%) trimmed read pairs available after processing
41224810 (99.21%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 19	       1	  0.00%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       2	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       1	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       1	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	       1	  0.00%
 67	       5	  0.00%
 68	       1	  0.00%
 69	       2	  0.00%
 70	       1	  0.00%
 71	       4	  0.00%
 72	       5	  0.00%
 73	       2	  0.00%
 74	       3	  0.00%
 75	       4	  0.00%
 76	       4	  0.00%
 77	       2	  0.00%
 78	       1	  0.00%
 79	       8	  0.00%
 80	       6	  0.00%
 81	       6	  0.00%
 82	      13	  0.00%
 83	      11	  0.00%
 84	      13	  0.00%
 85	      10	  0.00%
 86	       4	  0.00%
 87	      15	  0.00%
 88	      17	  0.00%
 89	      13	  0.00%
 90	      19	  0.00%
 91	      17	  0.00%
 92	      11	  0.00%
 93	      22	  0.00%
 94	      17	  0.00%
 95	      21	  0.00%
 96	      33	  0.00%
 97	      30	  0.00%
 98	      34	  0.00%
 99	      34	  0.00%
100	      39	  0.00%
101	      43	  0.00%
102	      47	  0.00%
103	      43	  0.00%
104	      42	  0.00%
105	      51	  0.00%
106	      61	  0.00%
107	      69	  0.00%
108	      72	  0.00%
109	      78	  0.00%
110	      78	  0.00%
111	      87	  0.00%
112	      77	  0.00%
113	      97	  0.00%
114	      93	  0.00%
115	     129	  0.00%
116	     138	  0.00%
117	     125	  0.00%
118	     149	  0.00%
119	     135	  0.00%
120	     140	  0.00%
121	     166	  0.00%
122	     174	  0.00%
123	     187	  0.00%
124	     207	  0.00%
125	     208	  0.00%
126	     208	  0.00%
127	     247	  0.00%
128	     259	  0.00%
129	     281	  0.00%
130	     294	  0.00%
131	     275	  0.00%
132	     347	  0.00%
133	     370	  0.00%
134	     366	  0.00%
135	     407	  0.00%
136	     436	  0.00%
137	     494	  0.00%
138	     453	  0.00%
139	     487	  0.00%
140	     482	  0.00%
141	   29760	  0.07%
142	   30594	  0.07%
143	   32219	  0.08%
144	   33749	  0.08%
145	   35358	  0.09%
146	   37217	  0.09%
147	   38388	  0.09%
148	   40595	  0.10%
149	   42409	  0.10%
150	41224810	 99.21%
41553635 reads passed initial QC


criterion=sequence-density
sequence-density=2.18
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=37
prefix-density=2.20
prefix-fanout=1.9
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=43
fanout-score=173.96
fanout-score-rank=1
prefix-density=0.90
prefix-fanout=1.0
sequence=TGGTAAAAGCTAGCTTTTTAGAGGGAACAGCTTTTTAACAAGAAAACCCAAGCAACTGCTGCCATGGCGGGGAAGCCAGTCCTTCATTACTTTGATGGCAGGGGAAGAATGGAGCCTATCCGGTGGCTCTTGGCTGCAGCTGGTGTGGAGTTTGAAGAAAAATTTCTGAAAACTCGGGATGACCTGGCAAGGTTACGAAGTGATGGGAGTCTGATGTTCCAGCAAGTGCCCATGGTAGAGATCGACGGGATGAAACTGGTGCAGACCAAAGCCATTCTCAACTACATTGCCTCCAAATACAACCTCTATGGGAAGGACATGAAGGAGAGAGCCATCATTGACATGTACACAGAAGGAGTGGCGGATCTGGAGATAATGATTCTCTATTACCCCCACATGCCCCCTGAGGAGAAAGAGGCAAGCCTTGCCAAGATCAAGGAACAAACCAGGAACCGTTACTTCCCTGCCTTTGAAAAGGTGTTGAAGAGCCATGGACAAGATTATCTCGTTG


criterion=sequence-density
sequence-density=2.20
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=35
prefix-density=2.22
prefix-fanout=2.0
sequence=TTATTGGATCAACA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=31
fanout-score=75.57
fanout-score-rank=1
prefix-density=1.83
prefix-fanout=1.0
sequence=CCTTCCTTTAATCTTAAGTAAAAGAGACACAGGGATTCAAAAATAAAAATTTCTTTCCCCATTCCCAGGCCTGTACCCAGTGCCCTCCATACCACCCTTCCCCTCTCTAACAGAAGCAAGGGAGGTTCAGCTTAACAGCCGCTGGGGGGGGTCAGAGGGGGGCTTCTGAGCTCAGTGTTGGTCTCTTTCCAAATATAAATACATGTGTCAAAACTGGGAACTCCTCCACACCCGTCACCCTGACGCCCTCCATTTCTGCTGGTGTTCGGGATGGGGAAGCCAGGCACCGACTGGCTGGGGTTTACTGCACACTTTGGGCATGGGCCCCACCAGTCTCCTGCTGCTCGTTGTAGAAGAGATGGCACTCGGGGTCTCCCCGGATGGTGGGAGCTCCCTGGATGGGCTTCCCGGTATTGGGGTTCACACACCAGCACTCCCCGCGCTGTCCGTTCAGAGACATCTTGCACTGCTTAAGGTTGTACCGGCCATGCTTGTCACAGTTGGGGATGTG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x TTATTGGATCAACA -y TTATTGGATCAACA -o SRR25558758 SRR25558758_1.fastq SRR25558758_2.fastq
Input file:	SRR25558758_1.fastq
Paired file:	SRR25558758_2.fastq
trimmed:	SRR25558758-trimmed-pair1.fastq, SRR25558758-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TTATTGGATCAACA
-- paired 3' end adapter sequence (-y):	TTATTGGATCAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Sun May 24 16:10:46 2026 >> started

Sun May 24 16:11:07 2026 >> done (20.314s)
13851212 read pairs processed; of these:
      45 ( 0.00%) short read pairs filtered out after trimming by size control
     204 ( 0.00%) empty read pairs filtered out after trimming by size control
13850963 (100.00%) read pairs available; of these:
      32 ( 0.00%) trimmed read pairs available after processing
13850931 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 67	       3	  0.00%
 68	       0	  0.00%
 69	       1	  0.00%
 70	       0	  0.00%
 71	       2	  0.00%
 72	       2	  0.00%
 73	       1	  0.00%
 74	       2	  0.00%
 75	       3	  0.00%
 76	       0	  0.00%
 77	       1	  0.00%
 78	       0	  0.00%
 79	       5	  0.00%
 80	       3	  0.00%
 81	       2	  0.00%
 82	       4	  0.00%
 83	       4	  0.00%
 84	       5	  0.00%
 85	       2	  0.00%
 86	       2	  0.00%
 87	       4	  0.00%
 88	       3	  0.00%
 89	       2	  0.00%
 90	       6	  0.00%
 91	       5	  0.00%
 92	       4	  0.00%
 93	       7	  0.00%
 94	       5	  0.00%
 95	       2	  0.00%
 96	      11	  0.00%
 97	      12	  0.00%
 98	      11	  0.00%
 99	      13	  0.00%
100	      12	  0.00%
101	      17	  0.00%
102	      19	  0.00%
103	      12	  0.00%
104	      16	  0.00%
105	      18	  0.00%
106	      23	  0.00%
107	      16	  0.00%
108	      26	  0.00%
109	      30	  0.00%
110	      26	  0.00%
111	      36	  0.00%
112	      32	  0.00%
113	      33	  0.00%
114	      31	  0.00%
115	      42	  0.00%
116	      42	  0.00%
117	      42	  0.00%
118	      52	  0.00%
119	      48	  0.00%
120	      58	  0.00%
121	      71	  0.00%
122	      58	  0.00%
123	      69	  0.00%
124	      71	  0.00%
125	      84	  0.00%
126	      74	  0.00%
127	      82	  0.00%
128	      82	  0.00%
129	      90	  0.00%
130	      97	  0.00%
131	      94	  0.00%
132	     113	  0.00%
133	     126	  0.00%
134	     120	  0.00%
135	     144	  0.00%
136	     151	  0.00%
137	     175	  0.00%
138	     145	  0.00%
139	     160	  0.00%
140	     180	  0.00%
141	   10042	  0.07%
142	   10314	  0.07%
143	   10761	  0.08%
144	   11459	  0.08%
145	   12040	  0.09%
146	   12799	  0.09%
147	   12902	  0.09%
148	   13703	  0.10%
149	   14327	  0.10%
150	13739672	 99.20%


criterion=sequence-density
sequence-density=0.34
sequence-density-rank=1
fanout-score=3.54
fanout-score-rank=26
prefix-density=0.41
prefix-fanout=2.9
sequence=CGCCTGCAGGCG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=73.29
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=7.7
sequence=CTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGCTCTGTGGGCCGTGCTGTTGGTCACATTGCTGACAGGATGCCTAGCCGAGGGAGAGCCGGAGGTGACAGATCAGCTCGAGTGGCAAAGCAACCAACCCTGGGAGCAGGCCCTGAACCGCTTCTGGGATTACCTGCGCTGGGTGCAGACGCTGTCTGACCAGGTCCAGGAAGAGCTGCAGAGCTCCCAAGTCACACAAGAACTGACGGCACTGATGGAGGACACTATGACGGAAGTAAAGGCTTACAAAAAGGAGCTGGAGGAACAGCTGGGTCCAGTGGCGGAGGAGACACGGGCCAGGCTGGGCAAAGAGGTGCAGGCGGCACAGGCCCGACTCGGAGCCGACATGGAGGATCTACGCAACCGACTCGGGCAGTACCGCAACGAGGTGCACACCATGCTGGGCCAGAGCACAGAGGAGATACGGGCGCGGCTCTCCACACACCTGCG


criterion=sequence-density
sequence-density=0.34
sequence-density-rank=1
fanout-score=3.48
fanout-score-rank=29
prefix-density=0.41
prefix-fanout=2.9
sequence=CGCCTGCAGGCG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=58.20
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=7.1
sequence=CTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCACAATTGCGAAGATGAAGGCTCTGTGGGCCGTGCTGTTGGTCACATTGCTGACAGGATGCCTAGCCGAGGGAGAGCCGGAGGTGACAGATCAGCTCGAGTGGCAAAGCAACCAACCCTGGGAGCAGGCCCTGAACCGCTTCTGGGATTACCTGCGCTGGGTGCAGACGCTGTCTGACCAGGTCCAGGAAGAGCTGCAGAGCTCCCAAGTCACACAAGAACTGACGGCACTGATGGAGGACACTATGACGGAAGTAAAGGCTTACAAAAAGGAGCTGGAGGAACAGCTGGGTCCAGTGGCGGAGGAGACACGGGCCAGGCTGGGCAAAGAGGTGCAGGCGGCACAGGCCCGACTCGGAGCCGACATGGAGGATCTACGCAACCGACTCGGGCAGTACCGCAACGAGGTGCACACCATGCTGGGCCAGAGCACAGAGGAGATACGGGCGCGGCTCTCCACACACCTGCG
SRR25558758 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 24 16:16:31
                             Started mapping on |	May 24 16:16:32
                                    Finished on |	May 24 16:25:41
       Mapping speed, Million of reads per hour |	272.48

                          Number of input reads |	41553386
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	38341076
                        Uniquely mapped reads % |	92.27%
                          Average mapped length |	298.96
                       Number of splices: Total |	41390458
            Number of splices: Annotated (sjdb) |	41140734
                       Number of splices: GT/AG |	41080824
                       Number of splices: GC/AG |	262419
                       Number of splices: AT/AC |	18573
               Number of splices: Non-canonical |	28642
                      Mismatch rate per base, % |	0.14%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.63
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2593288
             % of reads mapped to multiple loci |	6.24%
        Number of reads mapped to too many loci |	34897
             % of reads mapped to too many loci |	0.08%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.36%
                     % of reads unmapped: other |	0.04%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	619022	619022	619022
N_multimapping	2593288	2593288	2593288
N_noFeature	2058803	19496537	19509896
N_ambiguous	3012683	864267	855322
UnstrandedReadsAssigned:33269590 PositiveStrandReadsAssigned:17980272 NegativeStrandReadsAssigned:17975858
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR25558758 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25558758-trimmed-pair1.fastq
                             SRR25558758-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 41,553,386 reads, 38,315,596 reads pseudoaligned
[quant] estimated average fragment length: 243.475
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,101 rounds

 109283 SRR25558758.ke.tsv
  52636 SRR25558758.se.tsv
 161919 total
==> SRR25558758.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8	0	0
ENSMUST00000196221.1	9	3.16667	0	0
ENSMUST00000179664.1	11	4.71429	0	0
ENSMUST00000178537.1	12	5.71429	0	0
ENSMUST00000178862.1	14	6	0	0
ENSMUST00000179520.1	11	4.71429	0	0
ENSMUST00000179883.1	16	8	0	0
ENSMUST00000195858.1	10	3.71429	0	0
ENSMUST00000179932.1	12	5.71429	0	0

==> SRR25558758.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25558758 completed mapping pipeline successfully
