Starting /dee2/code/volunteer_pipeline.sh SRR25656850
    current disk space = 1156639736
    free memory = 191428940 
SRR25656850 SRAfilesize
be51c8871f30b75b38fa62eefd2432ec  SRR25656850.sra
SRR25656850.sra file validated
SRR25656850 is paired end
SRR25656850 is conventional basespace
SRR25656850 read1 length is 51-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.7375	37.0	37.0	37.0	37.0	37.0
2	36.48	37.0	37.0	37.0	37.0	37.0
3	36.622	37.0	37.0	37.0	37.0	37.0
4	36.605	37.0	37.0	37.0	37.0	37.0
5	36.5895	37.0	37.0	37.0	37.0	37.0
6	36.6465	37.0	37.0	37.0	37.0	37.0
7	36.5035	37.0	37.0	37.0	37.0	37.0
8	36.59425	37.0	37.0	37.0	37.0	37.0
9	36.571	37.0	37.0	37.0	37.0	37.0
10-11	36.606	37.0	37.0	37.0	37.0	37.0
12-13	36.620000000000005	37.0	37.0	37.0	37.0	37.0
14-15	36.6065	37.0	37.0	37.0	37.0	37.0
16-17	36.61750000000001	37.0	37.0	37.0	37.0	37.0
18-19	36.571	37.0	37.0	37.0	37.0	37.0
20-21	36.60525	37.0	37.0	37.0	37.0	37.0
22-23	36.54125	37.0	37.0	37.0	37.0	37.0
24-25	36.637	37.0	37.0	37.0	37.0	37.0
26-27	36.519999999999996	37.0	37.0	37.0	37.0	37.0
28-29	36.570750000000004	37.0	37.0	37.0	37.0	37.0
30-31	36.52825	37.0	37.0	37.0	37.0	37.0
32-33	36.57175	37.0	37.0	37.0	37.0	37.0
34-35	36.478	37.0	37.0	37.0	37.0	37.0
36-37	36.419	37.0	37.0	37.0	37.0	37.0
38-39	36.510999999999996	37.0	37.0	37.0	37.0	37.0
40-41	36.425250000000005	37.0	37.0	37.0	37.0	37.0
42-43	36.42275	37.0	37.0	37.0	37.0	37.0
44-45	36.44975	37.0	37.0	37.0	37.0	37.0
46-47	36.50025	37.0	37.0	37.0	37.0	37.0
48-49	36.37675	37.0	37.0	37.0	37.0	37.0
50-51	36.46125	37.0	37.0	37.0	37.0	37.0
52-53	36.48805379183715	37.0	37.0	37.0	37.0	37.0
54-55	36.39469734867434	37.0	37.0	37.0	37.0	37.0
56-57	36.41695847923962	37.0	37.0	37.0	37.0	37.0
58-59	36.40520260130065	37.0	37.0	37.0	37.0	37.0
60-61	36.3951975987994	37.0	37.0	37.0	37.0	37.0
62-63	36.394790989088136	37.0	37.0	37.0	37.0	37.0
64-65	36.347764786062655	37.0	37.0	37.0	37.0	37.0
66-67	36.41276595744681	37.0	37.0	37.0	37.0	37.0
68-69	36.30194172467148	37.0	37.0	37.0	37.0	37.0
70-71	36.37642335535516	37.0	37.0	37.0	37.0	37.0
72-73	36.34912433647555	37.0	37.0	37.0	37.0	37.0
74-75	36.38606166959138	37.0	37.0	37.0	37.0	37.0
76-77	36.24865039059994	37.0	37.0	37.0	37.0	37.0
78-79	36.30605912125323	37.0	37.0	37.0	37.0	37.0
80-81	36.25060986711736	37.0	37.0	37.0	37.0	37.0
82-83	36.30259947060094	37.0	37.0	37.0	37.0	37.0
84-85	36.303917205004886	37.0	37.0	37.0	37.0	37.0
86-87	36.30499187883914	37.0	37.0	37.0	37.0	37.0
88-89	36.248273486383766	37.0	37.0	37.0	37.0	37.0
90-91	36.157588426201826	37.0	37.0	37.0	37.0	37.0
92-93	36.101612246327946	37.0	37.0	37.0	37.0	37.0
94-95	36.08923359532434	37.0	37.0	37.0	37.0	37.0
96-97	36.094490213049966	37.0	37.0	37.0	37.0	37.0
98-99	36.209126064646505	37.0	37.0	37.0	37.0	37.0
100-101	36.44212815280834	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	2.0
26	3.0
27	4.0
28	14.0
29	10.0
30	25.0
31	29.0
32	49.0
33	60.0
34	80.0
35	213.0
36	2380.0
37	1129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.425	11.5	11.225	36.85
2	22.461230615307652	21.735867933966986	28.68934467233617	27.113556778389196
3	21.025	29.349999999999998	18.65	30.975
4	25.974999999999998	37.574999999999996	19.8	16.650000000000002
5	28.525	33.975	24.4	13.100000000000001
6	27.250000000000004	35.775	20.775	16.2
7	15.325	25.525	40.725	18.425
8	17.72943235808952	27.656914228557138	33.23330832708177	21.380345086271568
9	16.575	21.425	34.699999999999996	27.3
10-11	20.5	36.5375	21.55	21.4125
12-13	20.1875	29.8375	29.2875	20.6875
14-15	21.275	24.4375	26.787499999999998	27.500000000000004
16-17	22.037499999999998	30.8125	23.674999999999997	23.474999999999998
18-19	22.0625	27.3875	23.150000000000002	27.400000000000002
20-21	18.775	28.3875	27.775	25.0625
22-23	23.2375	28.6375	26.1125	22.0125
24-25	24.4	26.1625	29.549999999999997	19.8875
26-27	22.225	27.987499999999997	24.75	25.0375
28-29	22.5625	25.9625	27.1375	24.337500000000002
30-31	20.275000000000002	27.700000000000003	26.387500000000003	25.637500000000003
32-33	18.7	33.650000000000006	22.6125	25.0375
34-35	22.225	34.2375	22.3625	21.175
36-37	23.0875	27.212500000000002	25.137500000000003	24.5625
38-39	22.175	25.837500000000002	26.8375	25.15
40-41	22.6	24.55	31.4625	21.3875
42-43	24.099999999999998	23.8375	31.025000000000002	21.0375
44-45	27.287499999999998	23.825	26.987499999999997	21.9
46-47	25.924999999999997	25.074999999999996	26.0375	22.9625
48-49	24.2625	29.675	22.925	23.1375
50-51	26.650000000000002	30.312499999999996	20.150000000000002	22.8875
52-53	21.395523321245467	26.50994122796049	26.297361510566464	25.797173940227587
54-55	24.19959979989995	23.724362181090545	29.052026013006504	23.024012006003
56-57	22.048524262131068	23.611805902951478	32.978989494747374	21.360680340170084
58-59	19.259629814907452	27.963981990995496	29.964982491245625	22.811405702851424
60-61	26.775887943971988	32.328664332166085	23.21160580290145	17.68384192096048
62-63	25.794345759319487	28.646484863647736	23.254941205904426	22.304228171128347
64-65	24.59016393442623	23.93943186084345	28.206732574145914	23.26367163058441
66-67	25.894868585732166	27.29662077596996	25.494367959949937	21.314142678347935
68-69	25.898559799624294	23.98246712586099	27.56418284283031	22.554790231684407
70-71	20.58123512463986	29.888513090316927	30.53989728172366	18.990354503319555
72-73	18.87218045112782	26.090225563909776	33.1328320802005	21.904761904761905
74-75	23.08849335673101	25.09400852343946	29.744296816244674	22.07320130358486
76-77	27.059561128526642	31.974921630094045	22.846394984326018	18.11912225705329
78-79	29.06422478675364	30.494229804315104	19.618665328650277	20.822880080280985
80-81	26.217980914113507	27.561526870919135	24.723756906077348	21.496735308890006
82-83	24.368797889712347	25.813340032659216	26.44140183394046	23.37646024368798
84-85	20.59600150886458	28.416949578775302	29.661762856783604	21.325286055576512
86-87	22.439208769056318	27.630086934610055	27.390701776489855	22.540002519843767
88-89	20.711851571374478	27.476965795784423	28.877950271361858	22.933232361479238
90-91	25.939992404101787	25.420939359412586	26.940119002405368	21.698949234080263
92-93	27.902550437761704	28.194391574673265	25.885039969547012	18.01801801801802
94-95	24.691358024691358	28.42051673666794	26.498663612065677	20.389461626575027
96-97	22.84911922389584	30.265509318355882	28.23589481746234	18.649476640285933
98-99	21.047708138447145	28.531337698783908	31.885607376720564	18.535346786048375
100-101	21.382636655948552	11.43265451947124	38.56734548052876	28.617363344051448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.5
19	2.0
20	2.0
21	1.5
22	1.0
23	1.0
24	1.5
25	3.0
26	3.5
27	3.5
28	4.0
29	9.0
30	12.5
31	9.5
32	14.5
33	17.0
34	23.0
35	25.5
36	33.0
37	60.0
38	83.5
39	127.5
40	157.0
41	182.5
42	164.5
43	176.5
44	297.5
45	312.0
46	386.0
47	408.5
48	324.5
49	260.0
50	152.5
51	130.0
52	121.5
53	104.5
54	72.0
55	56.5
56	79.5
57	58.5
58	28.0
59	31.0
60	33.0
61	18.5
62	5.5
63	4.0
64	2.0
65	0.5
66	0.0
67	1.5
68	1.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.025
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-51	1.0
52-53	1.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.0
64-65	1.0
66-67	2.0
68-69	1.0
70-71	1.0
72-73	2.0
74-75	1.0
76-77	1.0
78-79	4.0
80-81	2.0
82-83	3.0
84-85	6.0
86-87	8.0
88-89	12.0
90-91	8.0
92-93	12.0
94-95	12.0
96-97	43.0
98-99	566.0
100-101	3311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.20689655172414	21.6
2	15.249042145593869	9.950000000000001
3	5.440613026819923	5.325
4	1.7624521072796935	2.3
5	1.7624521072796935	2.875
6	1.7624521072796935	3.45
7	0.996168582375479	2.275
8	0.9195402298850575	2.4
9	0.7662835249042145	2.25
>10	4.444444444444445	27.200000000000003
>50	0.38314176245210724	8.1
>100	0.30651340996168586	12.275
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	146	3.65	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	119	2.9749999999999996	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	114	2.85	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	112	2.8000000000000003	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	83	2.075	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	69	1.725	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	65	1.625	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	55	1.375	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	52	1.3	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	47	1.175	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	47	1.175	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	44	1.0999999999999999	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	41	1.0250000000000001	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	40	1.0	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	39	0.975	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	38	0.95	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	31	0.775	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	29	0.7250000000000001	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	28	0.7000000000000001	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	28	0.7000000000000001	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	25	0.625	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	22	0.5499999999999999	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	21	0.525	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	20	0.5	No Hit
AGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTA	20	0.5	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	19	0.475	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	19	0.475	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	19	0.475	No Hit
GTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATA	18	0.44999999999999996	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	18	0.44999999999999996	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	17	0.42500000000000004	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	17	0.42500000000000004	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	16	0.4	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	16	0.4	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	16	0.4	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	15	0.375	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	15	0.375	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	14	0.35000000000000003	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCA	14	0.35000000000000003	No Hit
CTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGG	14	0.35000000000000003	No Hit
GTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGA	14	0.35000000000000003	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	13	0.325	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	13	0.325	No Hit
CTCCATTTGGCAGATGGTGAAACTGAGGCAGAGAATAAATTGCATGCCAA	13	0.325	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	13	0.325	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	13	0.325	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	13	0.325	No Hit
CCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAG	13	0.325	No Hit
CCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTA	13	0.325	No Hit
CGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTG	13	0.325	No Hit
AGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAA	12	0.3	No Hit
TTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGAC	12	0.3	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	12	0.3	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	12	0.3	No Hit
TTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATT	12	0.3	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	12	0.3	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	11	0.27499999999999997	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	11	0.27499999999999997	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	11	0.27499999999999997	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	11	0.27499999999999997	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	11	0.27499999999999997	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	11	0.27499999999999997	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	11	0.27499999999999997	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	11	0.27499999999999997	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	10	0.25	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	10	0.25	No Hit
GCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTA	10	0.25	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	9	0.22499999999999998	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	9	0.22499999999999998	No Hit
CTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCC	9	0.22499999999999998	No Hit
GGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGACCA	9	0.22499999999999998	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	9	0.22499999999999998	No Hit
GTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGG	9	0.22499999999999998	No Hit
CAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTT	9	0.22499999999999998	No Hit
CAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGA	9	0.22499999999999998	No Hit
TCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGC	9	0.22499999999999998	No Hit
GTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCA	9	0.22499999999999998	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	8	0.2	No Hit
GGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGA	8	0.2	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	8	0.2	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	8	0.2	No Hit
AGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTC	8	0.2	No Hit
TCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTT	8	0.2	No Hit
AGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTC	8	0.2	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	8	0.2	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	8	0.2	No Hit
AGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGC	8	0.2	No Hit
GTGGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGA	8	0.2	No Hit
CTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAA	8	0.2	No Hit
CATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTG	7	0.17500000000000002	No Hit
TGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAG	7	0.17500000000000002	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	7	0.17500000000000002	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	7	0.17500000000000002	No Hit
CCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGA	7	0.17500000000000002	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	7	0.17500000000000002	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	7	0.17500000000000002	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	7	0.17500000000000002	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	7	0.17500000000000002	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	7	0.17500000000000002	No Hit
TTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTC	7	0.17500000000000002	No Hit
CTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCG	7	0.17500000000000002	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	7	0.17500000000000002	No Hit
GCCAGATGCTGGTCAGATGTCTTTTCTCAATGGCTTTTTTATTATTTTAT	6	0.15	No Hit
CAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGC	6	0.15	No Hit
GGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	6	0.15	No Hit
ATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	6	0.15	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	6	0.15	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	6	0.15	No Hit
GTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGC	6	0.15	No Hit
TAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCT	6	0.15	No Hit
AGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTC	6	0.15	No Hit
TGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCA	6	0.15	No Hit
GTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTT	6	0.15	No Hit
TAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAA	6	0.15	No Hit
CTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCCAGAT	6	0.15	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	6	0.15	No Hit
ATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTGC	6	0.15	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATC	6	0.15	No Hit
CATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGAT	6	0.15	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	6	0.15	No Hit
GAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTT	6	0.15	No Hit
TTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCT	6	0.15	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	6	0.15	No Hit
TGAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCC	6	0.15	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	6	0.15	No Hit
AGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTC	5	0.125	No Hit
CATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCA	5	0.125	No Hit
GCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTAT	5	0.125	No Hit
TTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCA	5	0.125	No Hit
ACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCA	5	0.125	No Hit
GCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCT	5	0.125	No Hit
AAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGG	5	0.125	No Hit
GAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACT	5	0.125	No Hit
GGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCC	5	0.125	No Hit
GCAGTGAAGGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCA	5	0.125	No Hit
TCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGG	5	0.125	No Hit
CATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCA	5	0.125	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	5	0.125	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	5	0.125	No Hit
GTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGC	5	0.125	No Hit
GAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCA	5	0.125	No Hit
CTCCCTTCAGATGACTAATTTCCTCTCACTTCCCATGAAGAAGTCTTCCT	5	0.125	No Hit
CGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCA	5	0.125	No Hit
GCATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTT	5	0.125	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	5	0.125	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	5	0.125	No Hit
AATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCC	5	0.125	No Hit
AGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	20	0.0020447918	70.18125	6
TGGTAAC	20	0.0020447918	70.18125	1
TAACAGA	20	0.0020447918	70.18125	4
CAGACCC	20	0.0020447918	70.18125	7
AACAGAC	20	0.0020447918	70.18125	5
AGACCCA	20	0.0020447918	70.18125	8
GACCCAA	20	0.0020447918	70.18125	9
GTAACAG	20	0.0020447918	70.18125	3
GGTAACA	20	0.0020447918	70.18125	2
CACAAGA	70	1.8708524E-4	33.419643	1
ACAAGAA	75	2.8063531E-4	31.191666	2
CAAGAAT	85	5.847043E-4	27.522058	3
AGAATCC	85	5.847043E-4	27.522058	5
AAGAATC	85	5.847043E-4	27.522058	4
GAATCCA	90	8.169623E-4	25.993055	6
TCCATGT	90	8.169623E-4	25.993055	9
AATCCAT	100	0.0015114581	23.393751	7
ATCCATG	100	0.0015114581	23.393751	8
AGGTTTT	120	4.495513E-5	20.405764	94-95
CCATGTG	90	1.1799738E-4	18.195139	10-11
>>END_MODULE
SRR25656850 read2 length is 37-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656850_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	37-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3745	37.0	37.0	37.0	37.0	37.0
2	36.589	37.0	37.0	37.0	37.0	37.0
3	36.5145	37.0	37.0	37.0	37.0	37.0
4	36.577	37.0	37.0	37.0	37.0	37.0
5	36.4005	37.0	37.0	37.0	37.0	37.0
6	36.5445	37.0	37.0	37.0	37.0	37.0
7	36.562	37.0	37.0	37.0	37.0	37.0
8	36.6285	37.0	37.0	37.0	37.0	37.0
9	36.514	37.0	37.0	37.0	37.0	37.0
10-11	36.6395	37.0	37.0	37.0	37.0	37.0
12-13	36.62625	37.0	37.0	37.0	37.0	37.0
14-15	36.48675	37.0	37.0	37.0	37.0	37.0
16-17	36.56375	37.0	37.0	37.0	37.0	37.0
18-19	36.56825	37.0	37.0	37.0	37.0	37.0
20-21	36.570499999999996	37.0	37.0	37.0	37.0	37.0
22-23	36.5645	37.0	37.0	37.0	37.0	37.0
24-25	36.5565	37.0	37.0	37.0	37.0	37.0
26-27	36.524249999999995	37.0	37.0	37.0	37.0	37.0
28-29	36.49	37.0	37.0	37.0	37.0	37.0
30-31	36.38575	37.0	37.0	37.0	37.0	37.0
32-33	36.46025	37.0	37.0	37.0	37.0	37.0
34-35	36.429	37.0	37.0	37.0	37.0	37.0
36-37	36.447	37.0	37.0	37.0	37.0	37.0
38-39	36.406601650412604	37.0	37.0	37.0	37.0	37.0
40-41	36.402701350675336	37.0	37.0	37.0	37.0	37.0
42-43	36.36568284142071	37.0	37.0	37.0	37.0	37.0
44-45	36.41945972986493	37.0	37.0	37.0	37.0	37.0
46-47	36.38587113171297	37.0	37.0	37.0	37.0	37.0
48-49	36.288966725043785	37.0	37.0	37.0	37.0	37.0
50-51	36.29712637330851	37.0	37.0	37.0	37.0	37.0
52-53	36.344094094094096	37.0	37.0	37.0	37.0	37.0
54-55	36.34109109109109	37.0	37.0	37.0	37.0	37.0
56-57	36.322072072072075	37.0	37.0	37.0	37.0	37.0
58-59	36.31581581581581	37.0	37.0	37.0	37.0	37.0
60-61	36.32374019827086	37.0	37.0	37.0	37.0	37.0
62-63	36.38740933678365	37.0	37.0	37.0	37.0	37.0
64-65	36.356959117614565	37.0	37.0	37.0	37.0	37.0
66-67	36.320891783567134	37.0	37.0	37.0	37.0	37.0
68-69	36.253679208050215	37.0	37.0	37.0	37.0	37.0
70-71	36.309339795156724	37.0	37.0	37.0	37.0	37.0
72-73	36.25766539245018	37.0	37.0	37.0	37.0	37.0
74-75	36.24240903387704	37.0	37.0	37.0	37.0	37.0
76-77	36.341114457831324	37.0	37.0	37.0	37.0	37.0
78-79	36.242764377449525	37.0	37.0	37.0	37.0	37.0
80-81	36.19142026451089	37.0	37.0	37.0	37.0	37.0
82-83	36.219916241224055	37.0	37.0	37.0	37.0	37.0
84-85	36.291868183231294	37.0	37.0	37.0	37.0	37.0
86-87	36.201337467416096	37.0	37.0	37.0	37.0	37.0
88-89	36.136389009093335	37.0	37.0	37.0	37.0	37.0
90-91	36.23085924008074	37.0	37.0	37.0	37.0	37.0
92-93	36.259444324628774	37.0	37.0	37.0	37.0	37.0
94-95	36.200264630768416	37.0	37.0	37.0	37.0	37.0
96-97	36.046509991961244	37.0	37.0	37.0	37.0	37.0
98-99	36.160542795911276	37.0	37.0	37.0	37.0	37.0
100-101	36.344217501131055	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.0
24	1.0
25	0.0
26	5.0
27	6.0
28	9.0
29	17.0
30	14.0
31	24.0
32	41.0
33	68.0
34	91.0
35	289.0
36	2474.0
37	958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6	17.625	13.05	28.725
2	26.35	23.525	29.099999999999998	21.025
3	24.0	26.775	29.425	19.8
4	25.424999999999997	35.175	23.549999999999997	15.85
5	24.65	35.4	20.95	19.0
6	23.025000000000002	29.975	24.575	22.425
7	18.025	21.575	37.3	23.1
8	19.975	24.25	27.325	28.449999999999996
9	23.525	26.150000000000002	24.474999999999998	25.85
10-11	24.875	32.5375	20.7625	21.825
12-13	20.3125	27.6125	27.287499999999998	24.7875
14-15	21.05	27.437499999999996	27.950000000000003	23.5625
16-17	20.625	27.737499999999997	26.5	25.137500000000003
18-19	22.5	27.0	27.925	22.575
20-21	21.762500000000003	29.9875	27.150000000000002	21.099999999999998
22-23	25.25	25.587500000000002	24.4	24.762500000000003
24-25	20.2625	26.55	26.937499999999996	26.25
26-27	20.375	29.725	24.7375	25.162499999999998
28-29	21.8875	28.050000000000004	25.2625	24.8
30-31	20.4375	25.4875	28.0875	25.9875
32-33	22.400000000000002	26.450000000000003	27.1125	24.0375
34-35	26.224999999999998	25.025	25.137500000000003	23.6125
36-37	24.474999999999998	26.5	27.8375	21.1875
38-39	25.381345336334082	27.106776694173547	26.106526631657918	21.405351337834457
40-41	19.959979989995	26.625812906453227	29.33966983491746	24.074537268634316
42-43	23.71185592796398	25.987993996998497	25.350175087543768	24.949974987493746
44-45	24.899949974987493	25.83791895947974	26.513256628314156	22.748874437218607
46-47	22.86429018136335	28.2801751094434	25.666041275797376	23.18949343339587
48-49	21.916437327995997	25.106329747310486	29.00925694270703	23.967975981986488
50-51	18.778931565119482	26.41060928312273	26.810959589640937	27.999499562116853
52-53	21.80930930930931	28.628628628628626	26.476476476476474	23.085585585585587
54-55	20.12012012012012	28.265765765765767	28.428428428428425	23.185685685685687
56-57	20.75825825825826	28.84134134134134	26.714214214214216	23.686186186186188
58-59	20.683183183183182	26.45145145145145	31.106106106106107	21.75925925925926
60-61	22.32511575522463	27.831310224002003	24.35239644600175	25.49117757477162
62-63	19.764676430091377	27.813243209412942	28.576793090499436	23.845287269996245
64-65	23.187679979967445	26.86866157505947	26.630775009390263	23.31288343558282
66-67	23.38426853707415	27.880761523046093	23.86022044088176	24.874749498997996
68-69	21.922787666081724	27.751316119328152	24.32940586613186	25.996490348458263
70-71	25.11598746081505	25.56739811912226	25.655172413793103	23.661442006269592
72-73	24.97491219267436	27.508780732563977	23.733065730055195	23.783241344706475
74-75	18.92095357590966	28.318695106649937	28.72020075282309	24.040150564617317
76-77	22.552710843373493	26.581325301204817	26.029116465863456	24.836847389558233
78-79	21.49133818729601	26.537785588752193	25.633944263118252	26.336931960833542
80-81	22.998617569435716	28.968204097021488	27.673746386829208	20.359431946713585
82-83	23.349679366276877	26.732050798440838	24.95913491764114	24.95913491764114
84-85	18.904971680302076	27.85399622404028	28.470736312146006	24.770295783511642
86-87	23.07013118062563	30.663471241170537	24.318869828456105	21.94752774974773
88-89	21.673196006571466	32.048527739163404	25.33805130797422	20.940224946290915
90-91	20.99125364431487	27.49397895804284	25.630624920775762	25.884142476866522
92-93	23.21859519878064	24.18391972564461	26.53372285024768	26.063762225327068
94-95	23.75796178343949	28.43312101910828	26.47133757961783	21.337579617834397
96-97	23.02480184096139	28.816159549987212	25.926872922526208	22.232165686525185
98-99	23.815150321648943	25.46934488643823	26.716555074176185	23.998949717736643
100-101	18.861870989530562	11.685241472475514	34.160756501182036	35.29213103681189
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	1.0
23	1.0
24	3.0
25	4.5
26	4.5
27	5.5
28	7.5
29	9.5
30	12.0
31	13.0
32	19.0
33	30.5
34	29.0
35	20.0
36	29.0
37	94.5
38	129.5
39	105.0
40	122.0
41	128.5
42	128.5
43	141.5
44	144.0
45	162.0
46	292.0
47	439.5
48	417.5
49	290.5
50	178.5
51	187.5
52	214.0
53	229.5
54	170.5
55	62.0
56	49.0
57	36.0
58	16.5
59	14.5
60	21.5
61	20.0
62	10.5
63	5.5
64	7.5
65	5.5
66	1.0
67	1.0
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
36-37	1.0
38-39	1.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.0
48-49	0.0
50-51	1.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	1.0
62-63	1.0
64-65	2.0
66-67	2.0
68-69	2.0
70-71	1.0
72-73	2.0
74-75	1.0
76-77	0.0
78-79	4.0
80-81	3.0
82-83	3.0
84-85	6.0
86-87	9.0
88-89	12.0
90-91	8.0
92-93	11.0
94-95	12.0
96-97	59.0
98-99	367.0
100-101	3490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.433052792654934	20.4
2	15.455241009946441	10.100000000000001
3	5.738332058148432	5.625
4	3.2134659525631215	4.2
5	1.8362662586074983	3.0
6	1.6832440703902065	3.3000000000000003
7	1.6067329762815608	3.675
8	0.8416220351951033	2.1999999999999997
9	0.9946442234123948	2.9250000000000003
>10	5.891354246365723	37.9
>50	0.306044376434583	6.675000000000001
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	74	1.8499999999999999	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	73	1.825	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	64	1.6	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	56	1.4000000000000001	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	47	1.175	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	46	1.15	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	41	1.0250000000000001	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	40	1.0	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	39	0.975	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	37	0.9249999999999999	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	34	0.8500000000000001	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	33	0.8250000000000001	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	31	0.775	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	30	0.75	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	30	0.75	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	30	0.75	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	29	0.7250000000000001	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	28	0.7000000000000001	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	28	0.7000000000000001	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	25	0.625	No Hit
AGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTC	25	0.625	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	25	0.625	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	24	0.6	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	24	0.6	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	24	0.6	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	23	0.575	No Hit
GCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGG	22	0.5499999999999999	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	21	0.525	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	21	0.525	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	21	0.525	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	20	0.5	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	20	0.5	No Hit
CAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCT	20	0.5	No Hit
GGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	20	0.5	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	20	0.5	No Hit
CGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCT	19	0.475	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	19	0.475	No Hit
TGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATA	19	0.475	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	19	0.475	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	18	0.44999999999999996	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	18	0.44999999999999996	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	17	0.42500000000000004	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	17	0.42500000000000004	No Hit
ATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAA	17	0.42500000000000004	No Hit
ACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATG	17	0.42500000000000004	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	16	0.4	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	16	0.4	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	16	0.4	No Hit
GAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCA	16	0.4	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	16	0.4	No Hit
GCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTG	15	0.375	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	15	0.375	No Hit
TGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCT	15	0.375	No Hit
GGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATA	15	0.375	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	15	0.375	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	15	0.375	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	14	0.35000000000000003	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	14	0.35000000000000003	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	14	0.35000000000000003	No Hit
CGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACT	13	0.325	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	13	0.325	No Hit
TGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCT	13	0.325	No Hit
TGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCA	12	0.3	No Hit
AATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACC	12	0.3	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	12	0.3	No Hit
GAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTG	12	0.3	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	12	0.3	No Hit
CCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCT	12	0.3	No Hit
GCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCC	11	0.27499999999999997	No Hit
CTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	11	0.27499999999999997	No Hit
GCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTA	11	0.27499999999999997	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	11	0.27499999999999997	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	11	0.27499999999999997	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	10	0.25	No Hit
CTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCG	10	0.25	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	10	0.25	No Hit
CTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGG	10	0.25	No Hit
CTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCAC	10	0.25	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	10	0.25	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	10	0.25	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	10	0.25	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	9	0.22499999999999998	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	9	0.22499999999999998	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	9	0.22499999999999998	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	9	0.22499999999999998	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	9	0.22499999999999998	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	9	0.22499999999999998	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	9	0.22499999999999998	No Hit
TTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAA	9	0.22499999999999998	No Hit
GTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAA	9	0.22499999999999998	No Hit
GGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTG	9	0.22499999999999998	No Hit
GAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCT	9	0.22499999999999998	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	9	0.22499999999999998	No Hit
AGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATG	9	0.22499999999999998	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	8	0.2	No Hit
GATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCC	8	0.2	No Hit
ATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTT	8	0.2	No Hit
TCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATG	8	0.2	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	8	0.2	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	8	0.2	No Hit
ATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAG	8	0.2	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	8	0.2	No Hit
AAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCG	8	0.2	No Hit
GGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCG	8	0.2	No Hit
TATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGA	8	0.2	No Hit
AGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAA	7	0.17500000000000002	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	7	0.17500000000000002	No Hit
GGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTG	7	0.17500000000000002	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	7	0.17500000000000002	No Hit
TTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCT	7	0.17500000000000002	No Hit
CCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGA	7	0.17500000000000002	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	7	0.17500000000000002	No Hit
GCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCA	7	0.17500000000000002	No Hit
ACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACA	7	0.17500000000000002	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	7	0.17500000000000002	No Hit
AAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATT	7	0.17500000000000002	No Hit
ATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTG	7	0.17500000000000002	No Hit
ATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTC	7	0.17500000000000002	No Hit
AGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACA	7	0.17500000000000002	No Hit
GTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCG	7	0.17500000000000002	No Hit
GTCTAGGGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCGGCAAGA	7	0.17500000000000002	No Hit
AGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTT	7	0.17500000000000002	No Hit
CGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAG	7	0.17500000000000002	No Hit
GACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTAT	7	0.17500000000000002	No Hit
TCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGT	7	0.17500000000000002	No Hit
CCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAAC	7	0.17500000000000002	No Hit
GAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAG	6	0.15	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	6	0.15	No Hit
GTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCA	6	0.15	No Hit
GCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCA	6	0.15	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGACC	6	0.15	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	6	0.15	No Hit
AAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTG	6	0.15	No Hit
CTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCC	6	0.15	No Hit
AGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTT	6	0.15	No Hit
CCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTA	6	0.15	No Hit
GCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTT	6	0.15	No Hit
AGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTG	6	0.15	No Hit
GCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGG	6	0.15	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	6	0.15	No Hit
ACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCA	6	0.15	No Hit
AGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAAT	6	0.15	No Hit
CTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTC	6	0.15	No Hit
ATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAG	6	0.15	No Hit
CCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGG	6	0.15	No Hit
CGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCA	6	0.15	No Hit
CTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGT	6	0.15	No Hit
GGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATC	6	0.15	No Hit
TTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTT	5	0.125	No Hit
GATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATAT	5	0.125	No Hit
AGGACTTTAAGCAAGACTAGATTTGGGACTTTGGCATGCAATTTATTCTC	5	0.125	No Hit
CAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTAC	5	0.125	No Hit
CTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAG	5	0.125	No Hit
GGGAGAAACTGGCAAAGCAGGGCGAACTGCCCAGGGATGTGCGCAAGGCA	5	0.125	No Hit
TGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCA	5	0.125	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGCA	5	0.125	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGT	5	0.125	No Hit
CTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTAC	5	0.125	No Hit
GATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGA	5	0.125	No Hit
GTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAA	5	0.125	No Hit
AATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCG	5	0.125	No Hit
GAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAA	5	0.125	No Hit
TGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATG	5	0.125	No Hit
GGGGAGTGTCAGACGGGACCAGTACTCGTTTCACTTAGGGTGGAGGAGAG	5	0.125	No Hit
AGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTA	5	0.125	No Hit
GGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGT	5	0.125	No Hit
GGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCGGCAAGATACTCA	5	0.125	No Hit
CTCAGCACTCGCAATGCTTTGCTGCATGGTATGGGCACTGCCCAGGTTTA	5	0.125	No Hit
CTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTA	5	0.125	No Hit
TGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCA	5	0.125	No Hit
GCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAG	5	0.125	No Hit
CTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2026-05-21T15:53:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T15:54:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T15:55:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T15:55:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T15:55:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617776 spots for SRR25656850.sra
Written 23617776 spots for SRR25656850.sra
Read 23617777 spots for SRR25656850.sra
Written 23617777 spots for SRR25656850.sra
SRR ids: ['SRR25656850.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bjcl8nw1
SRR25656850.sra spots: 188942209
blocks: [[1, 23617776], [23617777, 47235552], [47235553, 70853328], [70853329, 94471104], [94471105, 118088880], [118088881, 141706656], [141706657, 165324432], [165324433, 188942209]]
SRR25656850 file size 45621396
SRR25656850 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25656850 SRR25656850_1.fastq SRR25656850_2.fastq
Input file:	SRR25656850_1.fastq
Paired file:	SRR25656850_2.fastq
trimmed:	SRR25656850-trimmed-pair1.fastq, SRR25656850-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 16:12:36 2026 >> started

Thu May 21 16:19:25 2026 >> done (409.073s)
188942209 read pairs processed; of these:
        2 ( 0.00%) short read pairs filtered out after trimming by size control
      400 ( 0.00%) empty read pairs filtered out after trimming by size control
188941807 (100.00%) read pairs available; of these:
    50066 ( 0.03%) trimmed read pairs available after processing
188891741 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	        1	  0.00%
 19	        4	  0.00%
 20	       93	  0.00%
 21	      132	  0.00%
 22	      116	  0.00%
 23	      158	  0.00%
 24	      171	  0.00%
 25	      260	  0.00%
 26	      247	  0.00%
 27	      330	  0.00%
 28	      380	  0.00%
 29	      407	  0.00%
 30	      508	  0.00%
 31	      572	  0.00%
 32	      593	  0.00%
 33	      735	  0.00%
 34	      739	  0.00%
 35	      816	  0.00%
 36	      924	  0.00%
 37	      902	  0.00%
 38	     1087	  0.00%
 39	     1208	  0.00%
 40	     1297	  0.00%
 41	     1549	  0.00%
 42	     1684	  0.00%
 43	     1691	  0.00%
 44	     1918	  0.00%
 45	     2083	  0.00%
 46	     2288	  0.00%
 47	     2711	  0.00%
 48	     3109	  0.00%
 49	     3708	  0.00%
 50	     4061	  0.00%
 51	     4304	  0.00%
 52	     4656	  0.00%
 53	     5127	  0.00%
 54	     5618	  0.00%
 55	     6156	  0.00%
 56	     6446	  0.00%
 57	     7086	  0.00%
 58	     8234	  0.00%
 59	    20964	  0.01%
 60	   135766	  0.07%
 61	   156234	  0.08%
 62	   128634	  0.07%
 63	   108227	  0.06%
 64	   122634	  0.06%
 65	    97363	  0.05%
 66	    70482	  0.04%
 67	    81309	  0.04%
 68	    58926	  0.03%
 69	    60847	  0.03%
 70	    67090	  0.04%
 71	    56583	  0.03%
 72	    56339	  0.03%
 73	    57730	  0.03%
 74	    60512	  0.03%
 75	    61754	  0.03%
 76	    64922	  0.03%
 77	    65889	  0.03%
 78	    67610	  0.04%
 79	    74602	  0.04%
 80	    84579	  0.04%
 81	    92377	  0.05%
 82	   102127	  0.05%
 83	   110580	  0.06%
 84	   117488	  0.06%
 85	   129576	  0.07%
 86	   146816	  0.08%
 87	   158887	  0.08%
 88	   163734	  0.09%
 89	   183941	  0.10%
 90	   213388	  0.11%
 91	   243119	  0.13%
 92	   266243	  0.14%
 93	   307821	  0.16%
 94	   348860	  0.18%
 95	   395394	  0.21%
 96	   496589	  0.26%
 97	   690954	  0.37%
 98	  2187339	  1.16%
 99	 15304099	  8.10%
100	 82249261	 43.53%
101	 83190109	 44.03%
188941807 reads passed initial QC


criterion=sequence-density
sequence-density=1.72
sequence-density-rank=1
fanout-score=2.33
fanout-score-rank=33
prefix-density=1.88
prefix-fanout=2.1
sequence=AAACAATTATTAG


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=18
fanout-score=100.58
fanout-score-rank=1
prefix-density=4.37
prefix-fanout=1.6
sequence=ATCAGTTTTAATTCCTAGGGAGG


criterion=sequence-density
sequence-density=2.26
sequence-density-rank=1
fanout-score=7.19
fanout-score-rank=6
prefix-density=4.59
prefix-fanout=3.5
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=8
fanout-score=29.76
fanout-score-rank=1
prefix-density=3.04
prefix-fanout=1.0
sequence=TATATTAATTTAATCTGAAGACG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AAACAATTATTAG -y AAGAAAAAGGTTCC -o SRR25656850 SRR25656850_1.fastq SRR25656850_2.fastq
Input file:	SRR25656850_1.fastq
Paired file:	SRR25656850_2.fastq
trimmed:	SRR25656850-trimmed-pair1.fastq, SRR25656850-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AAACAATTATTAG
-- paired 3' end adapter sequence (-y):	AAGAAAAAGGTTCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 16:43:08 2026 >> started

Thu May 21 16:46:02 2026 >> done (173.455s)
62980602 read pairs processed; of these:
      63 ( 0.00%) short read pairs filtered out after trimming by size control
      36 ( 0.00%) empty read pairs filtered out after trimming by size control
62980503 (100.00%) read pairs available; of these:
    3999 ( 0.01%) trimmed read pairs available after processing
62976504 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 19	       3	  0.00%
 20	      30	  0.00%
 21	      38	  0.00%
 22	      36	  0.00%
 23	      53	  0.00%
 24	      59	  0.00%
 25	      85	  0.00%
 26	      62	  0.00%
 27	     107	  0.00%
 28	     132	  0.00%
 29	     141	  0.00%
 30	     164	  0.00%
 31	     212	  0.00%
 32	     197	  0.00%
 33	     228	  0.00%
 34	     209	  0.00%
 35	     248	  0.00%
 36	     272	  0.00%
 37	     287	  0.00%
 38	     334	  0.00%
 39	     378	  0.00%
 40	     420	  0.00%
 41	     514	  0.00%
 42	     511	  0.00%
 43	     576	  0.00%
 44	     592	  0.00%
 45	     648	  0.00%
 46	     770	  0.00%
 47	     871	  0.00%
 48	    1043	  0.00%
 49	    1227	  0.00%
 50	    1376	  0.00%
 51	    1401	  0.00%
 52	    1510	  0.00%
 53	    1631	  0.00%
 54	    1770	  0.00%
 55	    2073	  0.00%
 56	    2164	  0.00%
 57	    2329	  0.00%
 58	    2661	  0.00%
 59	    7229	  0.01%
 60	   44748	  0.07%
 61	   51935	  0.08%
 62	   42741	  0.07%
 63	   36177	  0.06%
 64	   40792	  0.06%
 65	   32643	  0.05%
 66	   23626	  0.04%
 67	   27253	  0.04%
 68	   19729	  0.03%
 69	   20209	  0.03%
 70	   22048	  0.04%
 71	   18678	  0.03%
 72	   18762	  0.03%
 73	   19095	  0.03%
 74	   19998	  0.03%
 75	   20314	  0.03%
 76	   21603	  0.03%
 77	   21864	  0.03%
 78	   22601	  0.04%
 79	   24368	  0.04%
 80	   28391	  0.05%
 81	   30714	  0.05%
 82	   33738	  0.05%
 83	   36565	  0.06%
 84	   39422	  0.06%
 85	   43400	  0.07%
 86	   49565	  0.08%
 87	   53612	  0.09%
 88	   55730	  0.09%
 89	   60919	  0.10%
 90	   70791	  0.11%
 91	   80318	  0.13%
 92	   88148	  0.14%
 93	   99908	  0.16%
 94	  116542	  0.19%
 95	  133558	  0.21%
 96	  166403	  0.26%
 97	  232366	  0.37%
 98	  724461	  1.15%
 99	 5084588	  8.07%
100	27402231	 43.51%
101	27765358	 44.09%


criterion=sequence-density
sequence-density=1.58
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=33
prefix-density=1.66
prefix-fanout=1.9
sequence=GCTCCGGCGAGGGAGGCATCCGC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=31
fanout-score=37.30
fanout-score-rank=1
prefix-density=0.25
prefix-fanout=3.5
sequence=AGCACACAGCAGCGAGGCGCAACGTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGAAGCCGGCGAGAGAAGACCACGCCAACGGGGGTCGGGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGAGGACGAACCCACCGTGAACTCGGGCGGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGCGACTTGAACCCCGCGAGAGGGAGGAAC


criterion=sequence-density
sequence-density=1.29
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=33
prefix-density=1.32
prefix-fanout=2.0
sequence=GCGGATGCCTCCCTCGCCGGAGC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=64.55
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=7.7
sequence=TGTTCCAGAGACGGCCGCATCTTCTTGTGCAGTGCCAGCCTCGTCCCGTAGACAAAATGGTGAAGGTCGGTGTGAACGGATTTGGCCGTATTGGGCGCCTGGTCACCAGGGCTGCCATTTGCAGTGGCAAAGTGGAGATTGTTGCCATCAACGACCCCTTCATTGACCTCAACTACATGGTCTACATGTTCCAGTATGACTCCACTCACGGCAAATTCAACGGCACAGTCAAGGCCGAGAATGGGAAGCTTGTCATCAACGGGAAGCCCATCACCATCTTCCAGGAGCGAGACCCCACTAACATCAAATGGGGTGAGGC
SRR25656850 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 16:55:15
                             Started mapping on |	May 21 16:55:15
                                    Finished on |	May 21 17:14:43
       Mapping speed, Million of reads per hour |	582.35

                          Number of input reads |	188941708
                      Average input read length |	200
                                    UNIQUE READS:
                   Uniquely mapped reads number |	138378976
                        Uniquely mapped reads % |	73.24%
                          Average mapped length |	198.90
                       Number of splices: Total |	88970048
            Number of splices: Annotated (sjdb) |	88379144
                       Number of splices: GT/AG |	87872566
                       Number of splices: GC/AG |	881873
                       Number of splices: AT/AC |	82520
               Number of splices: Non-canonical |	133089
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.53
                        Insertion rate per base |	0.02%
                       Insertion average length |	1.73
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	29491634
             % of reads mapped to multiple loci |	15.61%
        Number of reads mapped to too many loci |	1163516
             % of reads mapped to too many loci |	0.62%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.43%
                     % of reads unmapped: other |	0.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	21911604	21911604	21911604
N_multimapping	29491634	29491634	29491634
N_noFeature	14441357	131467738	15406909
N_ambiguous	8455414	91688	2774146
UnstrandedReadsAssigned:115482205 PositiveStrandReadsAssigned:6819550 NegativeStrandReadsAssigned:120197921
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=100 echo kmer=95
SRR25656850 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25656850-trimmed-pair1.fastq
                             SRR25656850-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 188,941,708 reads, 112,701,752 reads pseudoaligned
[quant] estimated average fragment length: 198.357
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,356 rounds

 109283 SRR25656850.ke.tsv
  52636 SRR25656850.se.tsv
 161919 total
==> SRR25656850.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.5	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	2	0	0
ENSMUST00000178537.1	12	3	0	0
ENSMUST00000178862.1	14	5	0	0
ENSMUST00000179520.1	11	2	0	0
ENSMUST00000179883.1	16	4.5	0	0
ENSMUST00000195858.1	10	1	0	0
ENSMUST00000179932.1	12	3	0	0

==> SRR25656850.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	1
SRR25656850 completed mapping pipeline successfully
