Starting /dee2/code/volunteer_pipeline.sh SRR25656853
    current disk space = 1065080608
    free memory = 192473972 
SRR25656853 SRAfilesize
7d6602a64e4b02ee001d6a69f4e0f59f  SRR25656853.sra
SRR25656853.sra file validated
SRR25656853 is paired end
SRR25656853 is conventional basespace
SRR25656853 read1 length is 45-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	45-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6285	37.0	37.0	37.0	37.0	37.0
2	36.459	37.0	37.0	37.0	37.0	37.0
3	36.59	37.0	37.0	37.0	37.0	37.0
4	36.5225	37.0	37.0	37.0	37.0	37.0
5	36.606	37.0	37.0	37.0	37.0	37.0
6	36.6235	37.0	37.0	37.0	37.0	37.0
7	36.4805	37.0	37.0	37.0	37.0	37.0
8	36.543	37.0	37.0	37.0	37.0	37.0
9	36.582	37.0	37.0	37.0	37.0	37.0
10-11	36.56425	37.0	37.0	37.0	37.0	37.0
12-13	36.522000000000006	37.0	37.0	37.0	37.0	37.0
14-15	36.570750000000004	37.0	37.0	37.0	37.0	37.0
16-17	36.509	37.0	37.0	37.0	37.0	37.0
18-19	36.57325	37.0	37.0	37.0	37.0	37.0
20-21	36.553250000000006	37.0	37.0	37.0	37.0	37.0
22-23	36.52175	37.0	37.0	37.0	37.0	37.0
24-25	36.55	37.0	37.0	37.0	37.0	37.0
26-27	36.51775	37.0	37.0	37.0	37.0	37.0
28-29	36.53075	37.0	37.0	37.0	37.0	37.0
30-31	36.5075	37.0	37.0	37.0	37.0	37.0
32-33	36.501000000000005	37.0	37.0	37.0	37.0	37.0
34-35	36.45	37.0	37.0	37.0	37.0	37.0
36-37	36.48825	37.0	37.0	37.0	37.0	37.0
38-39	36.455749999999995	37.0	37.0	37.0	37.0	37.0
40-41	36.416	37.0	37.0	37.0	37.0	37.0
42-43	36.47775	37.0	37.0	37.0	37.0	37.0
44-45	36.44225	37.0	37.0	37.0	37.0	37.0
46-47	36.37126315095532	37.0	37.0	37.0	37.0	37.0
48-49	36.39294647323662	37.0	37.0	37.0	37.0	37.0
50-51	36.383191595797896	37.0	37.0	37.0	37.0	37.0
52-53	36.42296148074037	37.0	37.0	37.0	37.0	37.0
54-55	36.418209104552275	37.0	37.0	37.0	37.0	37.0
56-57	36.343171585792895	37.0	37.0	37.0	37.0	37.0
58-59	36.39739159814166	37.0	37.0	37.0	37.0	37.0
60-61	36.44873254507168	37.0	37.0	37.0	37.0	37.0
62-63	36.382883003447446	37.0	37.0	37.0	37.0	37.0
64-65	36.29504232537089	37.0	37.0	37.0	37.0	37.0
66-67	36.31652871833458	37.0	37.0	37.0	37.0	37.0
68-69	36.21399951347526	37.0	37.0	37.0	37.0	37.0
70-71	36.269897062515696	37.0	37.0	37.0	37.0	37.0
72-73	36.274546365219706	37.0	37.0	37.0	37.0	37.0
74-75	36.32351633223003	37.0	37.0	37.0	37.0	37.0
76-77	36.32241523774982	37.0	37.0	37.0	37.0	37.0
78-79	36.27523789261916	37.0	37.0	37.0	37.0	37.0
80-81	36.29002695176726	37.0	37.0	37.0	37.0	37.0
82-83	36.34596897183332	37.0	37.0	37.0	37.0	37.0
84-85	36.28395451934608	37.0	37.0	37.0	37.0	37.0
86-87	36.35944209589949	37.0	37.0	37.0	37.0	37.0
88-89	36.33147052046429	37.0	37.0	37.0	37.0	37.0
90-91	36.281130392361284	37.0	37.0	37.0	37.0	37.0
92-93	36.191843588501726	37.0	37.0	37.0	37.0	37.0
94-95	36.28492524115621	37.0	37.0	37.0	37.0	37.0
96-97	36.216580054532734	37.0	37.0	37.0	37.0	37.0
98-99	36.268268212816395	37.0	37.0	37.0	37.0	37.0
100-101	36.46092951183768	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	2.0
27	6.0
28	15.0
29	19.0
30	20.0
31	33.0
32	38.0
33	68.0
34	95.0
35	210.0
36	2341.0
37	1152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.925	8.975	11.425	41.675000000000004
2	21.9	17.75	32.85	27.500000000000004
3	23.175	23.65	22.400000000000002	30.775000000000002
4	24.95	32.675	23.45	18.925
5	27.55	33.1	22.85	16.5
6	23.625	36.6	20.150000000000002	19.625
7	15.25	25.775	41.15	17.825
8	17.733866933466732	28.16408204102051	30.365182591295646	23.736868434217108
9	15.75	24.375	34.275	25.6
10-11	19.4625	36.987500000000004	23.375	20.175
12-13	22.237499999999997	27.200000000000003	28.712500000000002	21.85
14-15	22.9375	23.625	27.425	26.0125
16-17	23.2375	26.950000000000003	28.237499999999997	21.575
18-19	21.637500000000003	25.174999999999997	26.974999999999998	26.2125
20-21	19.075	24.474999999999998	29.675	26.775
22-23	20.9875	26.6625	28.225	24.125
24-25	21.587500000000002	25.9875	30.975	21.45
26-27	22.1375	25.6	26.2875	25.974999999999998
28-29	22.0875	23.7	27.737499999999997	26.474999999999998
30-31	19.875	26.637499999999996	27.487499999999997	26.0
32-33	20.0625	30.55	26.450000000000003	22.9375
34-35	22.725	29.062500000000004	27.650000000000002	20.5625
36-37	23.2625	27.712500000000002	26.025	23.0
38-39	24.4	23.3125	28.1	24.1875
40-41	22.912499999999998	23.1375	28.625	25.324999999999996
42-43	23.6375	22.45	30.875000000000004	23.0375
44-45	24.5375	24.337500000000002	28.5625	22.5625
46-47	25.271976991371766	22.52094535450794	29.27347755408278	22.933600100037513
48-49	25.662831415707853	28.176588294147077	23.374187093546773	22.786393196598297
50-51	22.211105552776388	28.901950975487743	23.59929964982491	25.287643821910955
52-53	19.797398699349674	27.03851925962982	25.987993996998497	27.176088044022013
54-55	23.036518259129565	24.912456228114056	28.189094547273637	23.861930965482742
56-57	22.848924462231114	22.67383691845923	32.51625812906453	21.96098049024512
58-59	19.194194194194196	25.287787787787785	30.342842842842842	25.175175175175173
60-61	23.631809643080775	26.649968691296184	26.88791484032561	22.830306825297434
62-63	24.501817270334627	26.469482391277104	25.479383381376113	23.549316957012156
64-65	22.896551724137932	22.13166144200627	32.36363636363636	22.608150470219435
66-67	23.76473539001756	26.51116127414096	25.746175068974164	23.97792826686732
68-69	23.31911690918214	25.589563472152534	26.593075765178124	24.498243853487207
70-71	20.938990710519708	29.876977152899826	27.46673361787597	21.717298518704496
72-73	21.736399045106168	26.196758386732	30.405829878125388	21.661012690036436
74-75	23.91742195367573	24.83635448136959	26.435045317220546	24.81117824773414
76-77	25.916593171223383	27.592289278064758	24.87085800680358	21.620259543908276
78-79	27.232593340060546	26.109989909182644	25.529767911200807	21.127648839556006
80-81	26.00783520788576	25.502337924933656	26.664981675723492	21.824845191457097
82-83	23.335864338142244	24.879777271576817	28.499114148316885	23.285244241964058
84-85	20.335110434120335	29.195227215029195	30.76923076923077	19.7004315816197
86-87	21.219450101832994	26.285641547861506	28.30957230142566	24.185336048879837
88-89	22.38710502750416	24.24203658692593	29.205577587309712	24.165280798260202
90-91	26.628967998971852	24.379899755815448	28.376815319367694	20.614316925845007
92-93	27.53417590920815	24.47768893474336	26.38638122259479	21.6017539334537
94-95	24.44300518134715	22.642487046632123	29.054404145077722	23.860103626943005
96-97	23.6903831118061	26.76570237164451	27.000260620276258	22.54365389627313
98-99	22.605053371166058	25.90190514795298	30.185110120254024	21.30793136062694
100-101	20.711805555555557	11.197916666666668	33.85416666666667	34.236111111111114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	1.0
14	0.5
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	1.0
25	1.5
26	0.5
27	0.0
28	1.5
29	2.0
30	1.0
31	1.5
32	5.5
33	8.0
34	6.0
35	8.5
36	21.5
37	29.5
38	51.5
39	118.5
40	157.0
41	165.0
42	148.5
43	228.0
44	367.0
45	322.0
46	361.0
47	399.0
48	320.5
49	235.5
50	178.0
51	198.5
52	156.0
53	104.0
54	62.5
55	57.0
56	94.0
57	66.0
58	25.5
59	34.0
60	47.0
61	29.5
62	4.0
63	2.5
64	2.0
65	1.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.05
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
44-45	1.0
46-47	1.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.0
58-59	3.0
60-61	4.0
62-63	2.0
64-65	1.0
66-67	0.0
68-69	4.0
70-71	2.0
72-73	7.0
74-75	5.0
76-77	2.0
78-79	9.0
80-81	5.0
82-83	9.0
84-85	13.0
86-87	19.0
88-89	15.0
90-91	17.0
92-93	15.0
94-95	22.0
96-97	40.0
98-99	471.0
100-101	3332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.749999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.69565217391305	16.875
2	12.434782608695652	7.1499999999999995
3	6.260869565217392	5.4
4	4.0	4.6
5	3.4782608695652173	5.0
6	2.6956521739130435	4.65
7	1.4782608695652173	2.9749999999999996
8	1.0434782608695654	2.4
9	1.4782608695652173	3.8249999999999997
>10	8.17391304347826	42.15
>50	0.26086956521739135	4.9750000000000005
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	84	2.1	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	60	1.5	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	55	1.375	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	48	1.2	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	47	1.175	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	43	1.075	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	41	1.0250000000000001	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	40	1.0	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	40	1.0	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	35	0.8750000000000001	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	33	0.8250000000000001	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	33	0.8250000000000001	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	31	0.775	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	31	0.775	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	30	0.75	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	27	0.675	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	26	0.65	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	25	0.625	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	25	0.625	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	24	0.6	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	22	0.5499999999999999	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	22	0.5499999999999999	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	22	0.5499999999999999	No Hit
CTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTC	21	0.525	No Hit
GCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACA	20	0.5	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	20	0.5	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	20	0.5	No Hit
GCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGG	20	0.5	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	20	0.5	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	20	0.5	No Hit
GTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAA	19	0.475	No Hit
CCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGT	19	0.475	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	19	0.475	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	19	0.475	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	19	0.475	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	19	0.475	No Hit
GCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAA	19	0.475	No Hit
GCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGAT	18	0.44999999999999996	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	18	0.44999999999999996	No Hit
GTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGG	17	0.42500000000000004	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	17	0.42500000000000004	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	16	0.4	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	16	0.4	No Hit
CTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTC	16	0.4	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	16	0.4	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	16	0.4	No Hit
GTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCA	16	0.4	No Hit
TGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAAT	16	0.4	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	16	0.4	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	15	0.375	No Hit
CCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTC	15	0.375	No Hit
GGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGAT	14	0.35000000000000003	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	14	0.35000000000000003	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	14	0.35000000000000003	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGC	14	0.35000000000000003	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	14	0.35000000000000003	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	14	0.35000000000000003	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	13	0.325	No Hit
TGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTT	13	0.325	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	13	0.325	No Hit
GAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTA	13	0.325	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	13	0.325	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	13	0.325	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	13	0.325	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	13	0.325	No Hit
CCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCAC	13	0.325	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	13	0.325	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	12	0.3	No Hit
GCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTC	12	0.3	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	12	0.3	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	12	0.3	No Hit
TCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCAC	12	0.3	No Hit
GTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGC	12	0.3	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	12	0.3	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	11	0.27499999999999997	No Hit
CACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGC	11	0.27499999999999997	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	11	0.27499999999999997	No Hit
GTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGG	11	0.27499999999999997	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	11	0.27499999999999997	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	11	0.27499999999999997	No Hit
AGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTC	11	0.27499999999999997	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	11	0.27499999999999997	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	11	0.27499999999999997	No Hit
CCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTA	11	0.27499999999999997	No Hit
GCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCA	11	0.27499999999999997	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	10	0.25	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	10	0.25	No Hit
GTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGA	10	0.25	No Hit
ATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	10	0.25	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	10	0.25	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	10	0.25	No Hit
TTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCT	10	0.25	No Hit
GCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCA	10	0.25	No Hit
GGGATTCGTATGTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGG	10	0.25	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	10	0.25	No Hit
CAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCC	10	0.25	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	10	0.25	No Hit
TTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCT	9	0.22499999999999998	No Hit
GGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATG	9	0.22499999999999998	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	9	0.22499999999999998	No Hit
CCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCC	9	0.22499999999999998	No Hit
AGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCC	9	0.22499999999999998	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	9	0.22499999999999998	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	9	0.22499999999999998	No Hit
CCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGA	9	0.22499999999999998	No Hit
CAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTT	9	0.22499999999999998	No Hit
GGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATT	9	0.22499999999999998	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	9	0.22499999999999998	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	9	0.22499999999999998	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	9	0.22499999999999998	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	9	0.22499999999999998	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	9	0.22499999999999998	No Hit
GCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTA	9	0.22499999999999998	No Hit
CTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCG	9	0.22499999999999998	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	8	0.2	No Hit
GCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGT	8	0.2	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	8	0.2	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	8	0.2	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	8	0.2	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	8	0.2	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	8	0.2	No Hit
ATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGG	8	0.2	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	8	0.2	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	8	0.2	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	8	0.2	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	8	0.2	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	7	0.17500000000000002	No Hit
CTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACA	7	0.17500000000000002	No Hit
CCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGG	7	0.17500000000000002	No Hit
AGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAA	7	0.17500000000000002	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	7	0.17500000000000002	No Hit
TTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGAC	7	0.17500000000000002	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	7	0.17500000000000002	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	7	0.17500000000000002	No Hit
ATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTGC	7	0.17500000000000002	No Hit
TTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTC	7	0.17500000000000002	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	7	0.17500000000000002	No Hit
CCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTT	7	0.17500000000000002	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	7	0.17500000000000002	No Hit
TTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTC	7	0.17500000000000002	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	7	0.17500000000000002	No Hit
GTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGA	7	0.17500000000000002	No Hit
AGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACT	7	0.17500000000000002	No Hit
ACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCG	6	0.15	No Hit
CCTAAATCAATGAGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTG	6	0.15	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	6	0.15	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	6	0.15	No Hit
CTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTC	6	0.15	No Hit
CCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTA	6	0.15	No Hit
CCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAA	6	0.15	No Hit
GCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCA	6	0.15	No Hit
GGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	6	0.15	No Hit
GTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTT	6	0.15	No Hit
GTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTT	6	0.15	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	6	0.15	No Hit
AGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTC	6	0.15	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	6	0.15	No Hit
TCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTT	6	0.15	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	6	0.15	No Hit
AGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGT	6	0.15	No Hit
CTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGG	6	0.15	No Hit
TAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATACT	6	0.15	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATC	6	0.15	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	6	0.15	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	6	0.15	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	6	0.15	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	6	0.15	No Hit
GCATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTT	6	0.15	No Hit
TTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAAT	6	0.15	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	6	0.15	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	6	0.15	No Hit
GGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAG	6	0.15	No Hit
GCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGC	6	0.15	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	6	0.15	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	5	0.125	No Hit
TTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGT	5	0.125	No Hit
GCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCCCTAGACTTCT	5	0.125	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	5	0.125	No Hit
GGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCA	5	0.125	No Hit
TGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAAT	5	0.125	No Hit
CGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAAT	5	0.125	No Hit
TGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTG	5	0.125	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCA	5	0.125	No Hit
CCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAG	5	0.125	No Hit
CATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATG	5	0.125	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	5	0.125	No Hit
GGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCG	5	0.125	No Hit
GTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTT	5	0.125	No Hit
TAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAA	5	0.125	No Hit
GGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTG	5	0.125	No Hit
GGCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAG	5	0.125	No Hit
CGTATGTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGT	5	0.125	No Hit
AGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGA	5	0.125	No Hit
CACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGAT	5	0.125	No Hit
TTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAA	5	0.125	No Hit
GCCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	5	0.125	No Hit
GTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGT	5	0.125	No Hit
TCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGG	5	0.125	No Hit
CAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGA	5	0.125	No Hit
ACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCT	5	0.125	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	5	0.125	No Hit
TCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCC	5	0.125	No Hit
AGCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTC	5	0.125	No Hit
GGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCC	5	0.125	No Hit
TGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTAT	5	0.125	No Hit
CGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCA	5	0.125	No Hit
CTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATA	5	0.125	No Hit
CGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATAGCT	5	0.125	No Hit
AGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTA	5	0.125	No Hit
AGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGCC	5	0.125	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	5	0.125	No Hit
AGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAAT	5	0.125	No Hit
GCCAGCGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAA	5	0.125	No Hit
CTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACCC	30	0.0042115883	30.87917	10-11
TACCCAC	30	0.0042115883	30.87917	12-13
CCCACAA	35	0.0089460155	26.467857	14-15
>>END_MODULE
SRR25656853 read2 length is 45-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656853_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	45-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1985	37.0	37.0	37.0	37.0	37.0
2	36.3395	37.0	37.0	37.0	37.0	37.0
3	36.278	37.0	37.0	37.0	37.0	37.0
4	36.3435	37.0	37.0	37.0	37.0	37.0
5	36.3545	37.0	37.0	37.0	37.0	37.0
6	36.2985	37.0	37.0	37.0	37.0	37.0
7	36.3985	37.0	37.0	37.0	37.0	37.0
8	36.3435	37.0	37.0	37.0	37.0	37.0
9	36.3315	37.0	37.0	37.0	37.0	37.0
10-11	36.29275	37.0	37.0	37.0	37.0	37.0
12-13	36.330749999999995	37.0	37.0	37.0	37.0	37.0
14-15	36.29425	37.0	37.0	37.0	37.0	37.0
16-17	36.412499999999994	37.0	37.0	37.0	37.0	37.0
18-19	36.35	37.0	37.0	37.0	37.0	37.0
20-21	36.377250000000004	37.0	37.0	37.0	37.0	37.0
22-23	36.29675	37.0	37.0	37.0	37.0	37.0
24-25	36.2645	37.0	37.0	37.0	37.0	37.0
26-27	36.26875	37.0	37.0	37.0	37.0	37.0
28-29	36.18000000000001	37.0	37.0	37.0	37.0	37.0
30-31	36.186	37.0	37.0	37.0	37.0	37.0
32-33	36.163	37.0	37.0	37.0	37.0	37.0
34-35	36.135000000000005	37.0	37.0	37.0	37.0	37.0
36-37	36.15225	37.0	37.0	37.0	37.0	37.0
38-39	36.1355	37.0	37.0	37.0	37.0	37.0
40-41	36.1605	37.0	37.0	37.0	37.0	37.0
42-43	36.0145	37.0	37.0	37.0	37.0	37.0
44-45	36.06825	37.0	37.0	37.0	37.0	37.0
46-47	36.019629844929966	37.0	37.0	37.0	37.0	37.0
48-49	36.10405202601301	37.0	37.0	37.0	37.0	37.0
50-51	36.01450725362682	37.0	37.0	37.0	37.0	37.0
52-53	36.024012006003005	37.0	37.0	37.0	37.0	37.0
54-55	35.950975487743875	37.0	37.0	37.0	37.0	37.0
56-57	35.95447723861931	37.0	37.0	37.0	37.0	37.0
58-59	36.116339200631344	37.0	37.0	37.0	37.0	37.0
60-61	36.05771085349673	37.0	37.0	37.0	37.0	37.0
62-63	35.9261892516307	37.0	37.0	37.0	37.0	37.0
64-65	35.90133800750459	37.0	37.0	37.0	37.0	37.0
66-67	35.9383149448345	37.0	37.0	37.0	37.0	37.0
68-69	35.959096682923835	37.0	37.0	37.0	37.0	37.0
70-71	36.013303212851405	37.0	37.0	37.0	37.0	37.0
72-73	35.92816051629317	37.0	37.0	37.0	37.0	37.0
74-75	35.874705939564876	37.0	37.0	37.0	37.0	37.0
76-77	35.92154676822581	37.0	37.0	37.0	37.0	37.0
78-79	35.93416769517127	37.0	37.0	37.0	37.0	37.0
80-81	35.910960821252715	37.0	37.0	37.0	37.0	37.0
82-83	35.87831869606216	37.0	37.0	37.0	37.0	37.0
84-85	35.81419776050298	37.0	37.0	37.0	37.0	37.0
86-87	35.88880232935969	37.0	37.0	37.0	37.0	37.0
88-89	35.74068562928278	37.0	37.0	37.0	37.0	37.0
90-91	35.8625860999899	37.0	37.0	37.0	37.0	37.0
92-93	35.85013358951296	37.0	37.0	37.0	37.0	37.0
94-95	35.75333317238017	37.0	37.0	37.0	37.0	37.0
96-97	35.75459238620067	37.0	37.0	37.0	37.0	37.0
98-99	35.88697230977638	37.0	37.0	37.0	37.0	37.0
100-101	36.021098664821636	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.0
24	2.0
25	9.0
26	2.0
27	10.0
28	15.0
29	23.0
30	32.0
31	48.0
32	57.0
33	91.0
34	147.0
35	475.0
36	2536.0
37	551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.925000000000004	17.45	11.5	29.125
2	26.625	23.275000000000002	27.650000000000002	22.45
3	24.099999999999998	27.425	26.950000000000003	21.525
4	28.975	32.300000000000004	21.525	17.2
5	28.225	37.85	16.125	17.8
6	23.775	34.075	22.15	20.0
7	22.35	22.225	33.2	22.225
8	24.575	24.0	24.275	27.150000000000002
9	24.675	26.174999999999997	24.099999999999998	25.05
10-11	26.7125	32.85	18.987499999999997	21.45
12-13	22.45	26.6	26.474999999999998	24.474999999999998
14-15	23.1625	28.175	26.8375	21.825
16-17	25.124999999999996	29.212500000000002	22.425	23.2375
18-19	22.3375	29.099999999999998	25.4625	23.1
20-21	23.724999999999998	29.775000000000002	24.3875	22.112499999999997
22-23	24.85	29.9625	22.15	23.0375
24-25	22.3375	28.575	25.25	23.8375
26-27	24.125	30.4875	22.625	22.7625
28-29	23.775	30.887500000000003	22.6375	22.7
30-31	22.925	27.975	27.1125	21.987499999999997
32-33	24.0375	29.5875	24.1375	22.237499999999997
34-35	27.750000000000004	27.450000000000003	24.1375	20.6625
36-37	24.6125	30.349999999999998	26.487500000000004	18.55
38-39	26.1125	27.175	26.3625	20.349999999999998
40-41	22.3625	28.275	26.937499999999996	22.425
42-43	25.775	31.112499999999997	21.75	21.3625
44-45	26.687499999999996	28.3375	23.8375	21.1375
46-47	24.559209703638864	30.198824559209704	23.708890834062775	21.533074903088657
48-49	25.087543771885944	28.101550775387697	25.175087543771884	21.635817908954476
50-51	21.053289967475607	28.884163122341754	26.344758568926697	23.71778834125594
52-53	23.28664332166083	30.25262631315658	26.663331665832917	19.797398699349674
54-55	24.187093546773387	29.052026013006504	25.46273136568284	21.298149074537267
56-57	20.74787393696848	29.102051025512754	28.08904452226113	22.061030515257627
58-59	23.455091318488865	29.00925694270703	25.519139354515886	22.016512384288216
60-61	26.33028671591336	28.22085889570552	22.398898209590584	23.049956178790534
62-63	21.764189951133943	29.106628242074926	26.462849267009148	22.666332539781983
64-65	25.072082236429736	26.501190923906233	24.921649743011155	23.505077096652876
66-67	24.874623871614844	27.657973921765294	23.495486459378135	23.971915747241727
68-69	22.987208427389014	28.404815650865313	26.285427639829447	22.322548281916227
70-71	24.33483935742972	28.589357429718877	24.008534136546185	23.067269076305223
72-73	24.962311557788944	26.218592964824122	25.464824120603012	23.354271356783922
74-75	20.78510317060896	31.039255158530448	26.585304479114242	21.59033719174635
76-77	23.83830751794484	27.23838307517945	25.462788061956932	23.460521344918774
78-79	23.197175995965708	27.710539586485122	25.542107917297024	23.550176500252142
80-81	25.097890615131995	30.26398888467854	23.822154856637614	20.815965643551852
82-83	24.158866683531496	26.865671641791046	25.183405008854038	23.792056665823424
84-85	20.748730964467004	30.431472081218274	28.08375634517766	20.736040609137056
86-87	25.413591244591498	29.61313311275134	24.879104097734793	20.094171544922375
88-89	22.304642537408874	32.58728737690242	22.266274459649573	22.841795626039136
90-91	23.307208017473982	28.60079660799178	25.337273544905564	22.75472182962868
92-93	23.807067320092855	27.58576218725819	24.542171782305907	24.06499871034305
94-95	23.24226336915706	29.76822478311537	24.990288747895896	21.99922309983167
96-97	23.860380307371713	30.945558739255013	24.355300859598856	20.838760093774418
98-99	26.11592271818787	26.19586942038641	24.30379746835443	23.384410393071285
100-101	20.3886925795053	11.819787985865725	35.14134275618375	32.650176678445234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.5
18	1.0
19	1.0
20	0.5
21	0.0
22	0.5
23	1.0
24	1.0
25	0.5
26	0.5
27	1.0
28	1.5
29	2.0
30	2.5
31	3.5
32	4.0
33	9.0
34	13.0
35	11.5
36	17.5
37	69.0
38	102.5
39	111.5
40	175.0
41	223.0
42	191.0
43	181.5
44	228.5
45	246.5
46	309.5
47	359.0
48	321.5
49	226.0
50	126.0
51	146.5
52	199.0
53	200.5
54	157.0
55	91.0
56	62.0
57	54.5
58	53.5
59	38.5
60	30.5
61	29.0
62	16.5
63	8.5
64	3.0
65	3.5
66	1.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.02501250625312656
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.025119316754584273
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
44-45	1.0
46-47	1.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	3.0
60-61	4.0
62-63	2.0
64-65	1.0
66-67	0.0
68-69	4.0
70-71	2.0
72-73	6.0
74-75	5.0
76-77	2.0
78-79	9.0
80-81	5.0
82-83	10.0
84-85	13.0
86-87	19.0
88-89	15.0
90-91	18.0
92-93	15.0
94-95	19.0
96-97	45.0
98-99	448.0
100-101	3353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.375000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.41563786008231	16.225
2	14.5679012345679	8.85
3	7.572016460905349	6.9
4	4.938271604938271	6.0
5	3.539094650205761	5.375
6	2.7160493827160495	4.95
7	1.9753086419753085	4.2
8	1.5637860082304527	3.8
9	1.316872427983539	3.5999999999999996
>10	8.31275720164609	38.75
>50	0.0823045267489712	1.35
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	54	1.35	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	41	1.0250000000000001	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	37	0.9249999999999999	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	37	0.9249999999999999	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	36	0.8999999999999999	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	33	0.8250000000000001	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	33	0.8250000000000001	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	32	0.8	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	28	0.7000000000000001	No Hit
GGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCT	23	0.575	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	22	0.5499999999999999	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	22	0.5499999999999999	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	22	0.5499999999999999	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	21	0.525	No Hit
GGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTT	21	0.525	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	21	0.525	No Hit
GGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	21	0.525	No Hit
GCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCAT	21	0.525	No Hit
GGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGC	20	0.5	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	20	0.5	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	19	0.475	No Hit
GTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGG	18	0.44999999999999996	No Hit
GGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGG	18	0.44999999999999996	No Hit
CATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA	18	0.44999999999999996	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	18	0.44999999999999996	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	18	0.44999999999999996	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	18	0.44999999999999996	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	18	0.44999999999999996	No Hit
AGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTC	17	0.42500000000000004	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	17	0.42500000000000004	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	17	0.42500000000000004	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	16	0.4	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	16	0.4	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	16	0.4	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	16	0.4	No Hit
GAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACT	15	0.375	No Hit
GTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGA	15	0.375	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	15	0.375	No Hit
CCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATC	15	0.375	No Hit
GCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGT	15	0.375	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	14	0.35000000000000003	No Hit
GGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACA	14	0.35000000000000003	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	13	0.325	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	13	0.325	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	13	0.325	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	13	0.325	No Hit
GTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACT	13	0.325	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	13	0.325	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	13	0.325	No Hit
GGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACAT	13	0.325	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	13	0.325	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	13	0.325	No Hit
GGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGG	13	0.325	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	13	0.325	No Hit
CTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGA	12	0.3	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	12	0.3	No Hit
CCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGAC	12	0.3	No Hit
GTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGG	12	0.3	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	12	0.3	No Hit
GGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGA	12	0.3	No Hit
GTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCA	12	0.3	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	12	0.3	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	12	0.3	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	12	0.3	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	12	0.3	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	12	0.3	No Hit
GGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATC	12	0.3	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	12	0.3	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	11	0.27499999999999997	No Hit
GCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGG	11	0.27499999999999997	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	11	0.27499999999999997	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	11	0.27499999999999997	No Hit
GCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAA	11	0.27499999999999997	No Hit
GTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGG	11	0.27499999999999997	No Hit
GACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAA	11	0.27499999999999997	No Hit
GCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTG	11	0.27499999999999997	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	11	0.27499999999999997	No Hit
CCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAG	11	0.27499999999999997	No Hit
GGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCA	11	0.27499999999999997	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	11	0.27499999999999997	No Hit
CGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCT	10	0.25	No Hit
GCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTG	10	0.25	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	10	0.25	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	10	0.25	No Hit
TAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGC	10	0.25	No Hit
TATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAA	10	0.25	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	10	0.25	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	10	0.25	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	10	0.25	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	10	0.25	No Hit
CCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTA	10	0.25	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	10	0.25	No Hit
ATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGA	10	0.25	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	10	0.25	No Hit
GGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGA	10	0.25	No Hit
CTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATA	10	0.25	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	10	0.25	No Hit
GTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTG	10	0.25	No Hit
GCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAG	10	0.25	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	10	0.25	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	10	0.25	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	10	0.25	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	9	0.22499999999999998	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	9	0.22499999999999998	No Hit
CAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTA	9	0.22499999999999998	No Hit
GCCCGACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGA	9	0.22499999999999998	No Hit
GTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCA	9	0.22499999999999998	No Hit
AGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCC	9	0.22499999999999998	No Hit
ATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAG	9	0.22499999999999998	No Hit
GCCCAGGGATGTGCGCAAGGCACAGGCCCGACTCCTTAGCCCTCCCACAC	9	0.22499999999999998	No Hit
GTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAA	9	0.22499999999999998	No Hit
CCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAA	9	0.22499999999999998	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	9	0.22499999999999998	No Hit
GTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGT	9	0.22499999999999998	No Hit
CCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAG	9	0.22499999999999998	No Hit
CCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAG	9	0.22499999999999998	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	9	0.22499999999999998	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	9	0.22499999999999998	No Hit
AAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAA	8	0.2	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	8	0.2	No Hit
TGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCT	8	0.2	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	8	0.2	No Hit
CTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	8	0.2	No Hit
GAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCA	8	0.2	No Hit
GGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATG	8	0.2	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	8	0.2	No Hit
CCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGAT	8	0.2	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	8	0.2	No Hit
CGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGG	8	0.2	No Hit
CCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTC	8	0.2	No Hit
TGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATG	8	0.2	No Hit
CTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGT	8	0.2	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	8	0.2	No Hit
GCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAAT	8	0.2	No Hit
GAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAG	8	0.2	No Hit
GAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCT	8	0.2	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	8	0.2	No Hit
TGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAG	7	0.17500000000000002	No Hit
TATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAAT	7	0.17500000000000002	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	7	0.17500000000000002	No Hit
CCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGA	7	0.17500000000000002	No Hit
TTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAA	7	0.17500000000000002	No Hit
TCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGA	7	0.17500000000000002	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	7	0.17500000000000002	No Hit
CAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACA	7	0.17500000000000002	No Hit
GCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGC	7	0.17500000000000002	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	7	0.17500000000000002	No Hit
GCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTA	7	0.17500000000000002	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGT	7	0.17500000000000002	No Hit
GAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAA	7	0.17500000000000002	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	7	0.17500000000000002	No Hit
CGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAG	7	0.17500000000000002	No Hit
GGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCT	7	0.17500000000000002	No Hit
AGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGG	7	0.17500000000000002	No Hit
CAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAA	7	0.17500000000000002	No Hit
GCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAG	7	0.17500000000000002	No Hit
GCTCAGGCGAAAGGAGGAGTTATGGGAGAAACTGGCAAAGCAGGGCGAAC	7	0.17500000000000002	No Hit
GGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGC	7	0.17500000000000002	No Hit
GCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCC	7	0.17500000000000002	No Hit
ACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATG	7	0.17500000000000002	No Hit
GTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGG	7	0.17500000000000002	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	6	0.15	No Hit
TCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATG	6	0.15	No Hit
AAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAA	6	0.15	No Hit
GGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCA	6	0.15	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	6	0.15	No Hit
GGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCG	6	0.15	No Hit
CAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTG	6	0.15	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	6	0.15	No Hit
TGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTA	6	0.15	No Hit
GTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTC	6	0.15	No Hit
AACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGA	6	0.15	No Hit
AGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGA	6	0.15	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	6	0.15	No Hit
GGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGA	6	0.15	No Hit
AAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTT	6	0.15	No Hit
GCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGG	6	0.15	No Hit
CATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTG	6	0.15	No Hit
ATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCA	6	0.15	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	6	0.15	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	6	0.15	No Hit
GGCCCGACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGG	6	0.15	No Hit
ACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCAT	6	0.15	No Hit
ACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGT	6	0.15	No Hit
GAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGC	6	0.15	No Hit
CAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACA	6	0.15	No Hit
ATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAG	6	0.15	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	6	0.15	No Hit
AGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTAC	6	0.15	No Hit
TGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCT	6	0.15	No Hit
CACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTT	6	0.15	No Hit
CAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGG	6	0.15	No Hit
ATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAA	6	0.15	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	6	0.15	No Hit
AGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTT	5	0.125	No Hit
CAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTAC	5	0.125	No Hit
GAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAG	5	0.125	No Hit
GCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATA	5	0.125	No Hit
CTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGT	5	0.125	No Hit
TCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTC	5	0.125	No Hit
ATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCG	5	0.125	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	5	0.125	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	5	0.125	No Hit
CTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCC	5	0.125	No Hit
AGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTT	5	0.125	No Hit
TGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATA	5	0.125	No Hit
GTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGA	5	0.125	No Hit
GCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTT	5	0.125	No Hit
TACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAG	5	0.125	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	5	0.125	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	5	0.125	No Hit
TTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCC	5	0.125	No Hit
TGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAG	5	0.125	No Hit
AGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACA	5	0.125	No Hit
ATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTAT	5	0.125	No Hit
TTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAA	5	0.125	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	5	0.125	No Hit
TTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGA	5	0.125	No Hit
GTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAG	5	0.125	No Hit
ATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACC	5	0.125	No Hit
GGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCA	5	0.125	No Hit
GTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGC	5	0.125	No Hit
GACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAA	5	0.125	No Hit
GAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTT	5	0.125	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	5	0.125	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	5	0.125	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	5	0.125	No Hit
CCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGA	5	0.125	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	5	0.125	No Hit
AGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACC	5	0.125	No Hit
CCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAG	5	0.125	No Hit
CGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGG	5	0.125	No Hit
GCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATA	5	0.125	No Hit
CTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGG	5	0.125	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	5	0.125	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	5	0.125	No Hit
AGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2026-05-21T16:54:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:54:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T16:55:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
Read 18077376 spots for SRR25656853.sra
Written 18077376 spots for SRR25656853.sra
Read 18077374 spots for SRR25656853.sra
Written 18077374 spots for SRR25656853.sra
SRR ids: ['SRR25656853.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_0jed_tk7
SRR25656853.sra spots: 144618994
blocks: [[1, 18077374], [18077375, 36154748], [36154749, 54232122], [54232123, 72309496], [72309497, 90386870], [90386871, 108464244], [108464245, 126541618], [126541619, 144618994]]
SRR25656853 file size 34769128
SRR25656853 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25656853 SRR25656853_1.fastq SRR25656853_2.fastq
Input file:	SRR25656853_1.fastq
Paired file:	SRR25656853_2.fastq
trimmed:	SRR25656853-trimmed-pair1.fastq, SRR25656853-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:04:54 2026 >> started

Thu May 21 17:08:38 2026 >> done (224.033s)
144618994 read pairs processed; of these:
        1 ( 0.00%) short read pairs filtered out after trimming by size control
      628 ( 0.00%) empty read pairs filtered out after trimming by size control
144618365 (100.00%) read pairs available; of these:
    47226 ( 0.03%) trimmed read pairs available after processing
144571139 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	        1	  0.00%
 19	        0	  0.00%
 20	       61	  0.00%
 21	       74	  0.00%
 22	       93	  0.00%
 23	      101	  0.00%
 24	      129	  0.00%
 25	      165	  0.00%
 26	      250	  0.00%
 27	      310	  0.00%
 28	      409	  0.00%
 29	      499	  0.00%
 30	      610	  0.00%
 31	      764	  0.00%
 32	      931	  0.00%
 33	     1004	  0.00%
 34	     1073	  0.00%
 35	     1133	  0.00%
 36	     1365	  0.00%
 37	     1587	  0.00%
 38	     1900	  0.00%
 39	     2146	  0.00%
 40	     2527	  0.00%
 41	     3127	  0.00%
 42	     3566	  0.00%
 43	     3660	  0.00%
 44	     3856	  0.00%
 45	     4329	  0.00%
 46	     4564	  0.00%
 47	     5874	  0.00%
 48	     6701	  0.00%
 49	     8223	  0.01%
 50	     8340	  0.01%
 51	     8880	  0.01%
 52	    10446	  0.01%
 53	    11680	  0.01%
 54	    12522	  0.01%
 55	    13122	  0.01%
 56	    14187	  0.01%
 57	    15125	  0.01%
 58	    17742	  0.01%
 59	    28009	  0.02%
 60	   121487	  0.08%
 61	   164867	  0.11%
 62	   122407	  0.08%
 63	   122392	  0.08%
 64	    93748	  0.06%
 65	    99792	  0.07%
 66	    88822	  0.06%
 67	   105033	  0.07%
 68	    77180	  0.05%
 69	    83039	  0.06%
 70	   102353	  0.07%
 71	    92965	  0.06%
 72	    74049	  0.05%
 73	    74319	  0.05%
 74	    77348	  0.05%
 75	    85022	  0.06%
 76	    93188	  0.06%
 77	    97900	  0.07%
 78	   104573	  0.07%
 79	   117928	  0.08%
 80	   129386	  0.09%
 81	   148082	  0.10%
 82	   162303	  0.11%
 83	   171066	  0.12%
 84	   184170	  0.13%
 85	   206484	  0.14%
 86	   224625	  0.16%
 87	   241581	  0.17%
 88	   236850	  0.16%
 89	   261760	  0.18%
 90	   302394	  0.21%
 91	   331023	  0.23%
 92	   343831	  0.24%
 93	   389894	  0.27%
 94	   455792	  0.32%
 95	   490542	  0.34%
 96	   566852	  0.39%
 97	   720625	  0.50%
 98	  1801689	  1.25%
 99	 10996620	  7.60%
100	 59832387	 41.37%
101	 64220912	 44.41%
144618365 reads passed initial QC


criterion=sequence-density
sequence-density=1.29
sequence-density-rank=1
fanout-score=3.63
fanout-score-rank=28
prefix-density=4.62
prefix-fanout=1.0
sequence=CCAAAAATCCACTTAATATATTG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=102.96
fanout-score-rank=1
prefix-density=0.28
prefix-fanout=1.9
sequence=ACCATTTCACAGCAAAATGAAGAGCCAAGGGTTATCTTTCAAGCTTTTATTTAAGTGCACTGACTTAAGAATGATTTAAATCTTGTTAAAAGCAGCCACATCCATGGACTGTACGTAGTCCTCAAAAGCAGTAATTTGCTCTTCCAGCATATCCGTTCCAACCTTATCATCTTCAACTACACACTGTATTTGAAGCTTTTTAATTCCATATCCCACTGGAACCAATTTAGAGGAGCCCCACACCAGGCCATCTGCTTGAATGCTTCGGACACACTCCTCTAGTTTTGTCATGTCCGTCTCATCATCCCAAGGCTTCACGTCTAGTAGGATGGAAGACTTCGCAACAACTGCAGGCTTTTTAGCTTTCTTGGACTCATACTGTGCAAGGCGTTCTTCTCGTAGCTTCTTTGCTTCCTCACTTTCCTCCTCATCATCAGATCCAAAGAGATCAATGTCGTCATCATCCTTAGCATCTGCAGCTCCACTTCCTGTGGTGTCTTCTACACTGCTA


criterion=sequence-density
sequence-density=2.72
sequence-density-rank=1
fanout-score=6.92
fanout-score-rank=9
prefix-density=5.43
prefix-fanout=3.5
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=45
fanout-score=27.49
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=1.2
sequence=ACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGTCTAGTAATATGATTTCACTATAATTCAATTACACTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGCCACCACACTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCGTACCAACACATGATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CCAAAAATCCACTTAATATATTG -y AAGAAAAAGGTTCC -o SRR25656853 SRR25656853_1.fastq SRR25656853_2.fastq
Input file:	SRR25656853_1.fastq
Paired file:	SRR25656853_2.fastq
trimmed:	SRR25656853-trimmed-pair1.fastq, SRR25656853-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CCAAAAATCCACTTAATATATTG
-- paired 3' end adapter sequence (-y):	AAGAAAAAGGTTCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:24:54 2026 >> started

Thu May 21 17:28:42 2026 >> done (227.628s)
48206122 read pairs processed; of these:
     116 ( 0.00%) short read pairs filtered out after trimming by size control
     449 ( 0.00%) empty read pairs filtered out after trimming by size control
48205557 (100.00%) read pairs available; of these:
   24803 ( 0.05%) trimmed read pairs available after processing
48180754 (99.95%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       1	  0.00%
 19	       0	  0.00%
 20	      23	  0.00%
 21	      19	  0.00%
 22	      32	  0.00%
 23	      36	  0.00%
 24	      37	  0.00%
 25	      62	  0.00%
 26	      73	  0.00%
 27	     106	  0.00%
 28	     166	  0.00%
 29	     159	  0.00%
 30	     221	  0.00%
 31	     255	  0.00%
 32	     308	  0.00%
 33	     335	  0.00%
 34	     364	  0.00%
 35	     408	  0.00%
 36	     471	  0.00%
 37	     525	  0.00%
 38	     663	  0.00%
 39	     704	  0.00%
 40	     839	  0.00%
 41	    1066	  0.00%
 42	    1193	  0.00%
 43	    1208	  0.00%
 44	    1347	  0.00%
 45	    1448	  0.00%
 46	    1567	  0.00%
 47	    1949	  0.00%
 48	    2334	  0.00%
 49	    2746	  0.01%
 50	    2855	  0.01%
 51	    2929	  0.01%
 52	    3564	  0.01%
 53	    3889	  0.01%
 54	    4322	  0.01%
 55	    4335	  0.01%
 56	    4824	  0.01%
 57	    5054	  0.01%
 58	    5976	  0.01%
 59	    9907	  0.02%
 60	   40944	  0.08%
 61	   55017	  0.11%
 62	   41091	  0.09%
 63	   40699	  0.08%
 64	   31534	  0.07%
 65	   34295	  0.07%
 66	   29823	  0.06%
 67	   35239	  0.07%
 68	   25782	  0.05%
 69	   27747	  0.06%
 70	   33804	  0.07%
 71	   30542	  0.06%
 72	   23753	  0.05%
 73	   25014	  0.05%
 74	   26275	  0.05%
 75	   29287	  0.06%
 76	   32126	  0.07%
 77	   33279	  0.07%
 78	   35767	  0.07%
 79	   40303	  0.08%
 80	   43993	  0.09%
 81	   49183	  0.10%
 82	   54033	  0.11%
 83	   57852	  0.12%
 84	   61795	  0.13%
 85	   69666	  0.14%
 86	   73384	  0.15%
 87	   80255	  0.17%
 88	   78540	  0.16%
 89	   88834	  0.18%
 90	  101557	  0.21%
 91	  111993	  0.23%
 92	  114678	  0.24%
 93	  130270	  0.27%
 94	  152196	  0.32%
 95	  162834	  0.34%
 96	  185189	  0.38%
 97	  242793	  0.50%
 98	  609212	  1.26%
 99	 3667007	  7.61%
100	19872223	 41.22%
101	21457429	 44.51%


criterion=sequence-density
sequence-density=1.44
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=31
prefix-density=1.51
prefix-fanout=1.9
sequence=GCTCCGGCGAGGGAGGCATCCGC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=73.82
fanout-score-rank=1
prefix-density=0.72
prefix-fanout=1.0
sequence=CCCGACCCCGAGAGACGCGCACCCGCGGCCGCTCCCGTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTAT


criterion=sequence-density
sequence-density=1.14
sequence-density-rank=1
fanout-score=2.07
fanout-score-rank=27
prefix-density=1.15
prefix-fanout=2.0
sequence=GCGGATGCCTCCCTCGCCGGAGC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=36.98
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=5.3
sequence=TGTTCCAGAGACGGCCGCATCTTCTTGTGCAGTGCCAGCCTCGTCCCGTAGACAAAATGGTGAAGGTCGGTGTGAACGGATTTGGCCGTATTGGGCGCCTGGTCACCAGGGCTGCCATTTGCAGTGGCAAAGTGGAGATTGTTGCCATCAACGACCCCTTCATTGACCTCAACTACATGGTCTACATGTTCCAGTATGACTCCACTCACGGCAAATTCAACGGCACAGTCAAGGCCGAGAATGGGAAGCTTGTCATCAACGGGAAGCCCATCACCATCTTCCAGGAGCGAGACCCCACTAACATCAAATGGGGTGAGGC
SRR25656853 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 17:37:23
                             Started mapping on |	May 21 17:37:23
                                    Finished on |	May 21 17:51:51
       Mapping speed, Million of reads per hour |	599.80

                          Number of input reads |	144617800
                      Average input read length |	199
                                    UNIQUE READS:
                   Uniquely mapped reads number |	96019690
                        Uniquely mapped reads % |	66.40%
                          Average mapped length |	197.91
                       Number of splices: Total |	63242064
            Number of splices: Annotated (sjdb) |	62812195
                       Number of splices: GT/AG |	62269373
                       Number of splices: GC/AG |	792764
                       Number of splices: AT/AC |	48273
               Number of splices: Non-canonical |	131654
                      Mismatch rate per base, % |	0.36%
                         Deletion rate per base |	0.05%
                        Deletion average length |	4.66
                        Insertion rate per base |	0.04%
                       Insertion average length |	2.22
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	29954698
             % of reads mapped to multiple loci |	20.71%
        Number of reads mapped to too many loci |	1345625
             % of reads mapped to too many loci |	0.93%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	11.91%
                     % of reads unmapped: other |	0.06%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	19369450	19369450	19369450
N_multimapping	29954698	29954698	29954698
N_noFeature	13072191	86072544	16724744
N_ambiguous	8573902	409873	2425252
UnstrandedReadsAssigned:74373597 PositiveStrandReadsAssigned:9537273 NegativeStrandReadsAssigned:76869694
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=100 echo kmer=95
SRR25656853 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25656853-trimmed-pair1.fastq
                             SRR25656853-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 144,617,800 reads, 71,531,028 reads pseudoaligned
[quant] estimated average fragment length: 179.059
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,286 rounds

 109283 SRR25656853.ke.tsv
  52636 SRR25656853.se.tsv
 161919 total
==> SRR25656853.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	2.66667	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	1.5	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	2.66667	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25656853.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25656853 completed mapping pipeline successfully
