Starting /dee2/code/volunteer_pipeline.sh SRR25656855
    current disk space = 1093498460
    free memory = 188891080 
SRR25656855 SRAfilesize
596a333ea65986f044d98fc9a52bcf30  SRR25656855.sra
SRR25656855.sra file validated
SRR25656855 is paired end
SRR25656855 is conventional basespace
SRR25656855 read1 length is 33-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	33-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6485	37.0	37.0	37.0	37.0	37.0
2	36.46625	37.0	37.0	37.0	37.0	37.0
3	36.6305	37.0	37.0	37.0	37.0	37.0
4	36.523	37.0	37.0	37.0	37.0	37.0
5	36.5645	37.0	37.0	37.0	37.0	37.0
6	36.5885	37.0	37.0	37.0	37.0	37.0
7	36.446	37.0	37.0	37.0	37.0	37.0
8	36.554	37.0	37.0	37.0	37.0	37.0
9	36.531	37.0	37.0	37.0	37.0	37.0
10-11	36.592749999999995	37.0	37.0	37.0	37.0	37.0
12-13	36.638	37.0	37.0	37.0	37.0	37.0
14-15	36.59425	37.0	37.0	37.0	37.0	37.0
16-17	36.5145	37.0	37.0	37.0	37.0	37.0
18-19	36.51275	37.0	37.0	37.0	37.0	37.0
20-21	36.58525	37.0	37.0	37.0	37.0	37.0
22-23	36.550250000000005	37.0	37.0	37.0	37.0	37.0
24-25	36.6275	37.0	37.0	37.0	37.0	37.0
26-27	36.559	37.0	37.0	37.0	37.0	37.0
28-29	36.543	37.0	37.0	37.0	37.0	37.0
30-31	36.564499999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.5115	37.0	37.0	37.0	37.0	37.0
34-35	36.52326163081541	37.0	37.0	37.0	37.0	37.0
36-37	36.472486243121566	37.0	37.0	37.0	37.0	37.0
38-39	36.51175587793897	37.0	37.0	37.0	37.0	37.0
40-41	36.516758379189596	37.0	37.0	37.0	37.0	37.0
42-43	36.42271135567783	37.0	37.0	37.0	37.0	37.0
44-45	36.456228114057026	37.0	37.0	37.0	37.0	37.0
46-47	36.46423211605803	37.0	37.0	37.0	37.0	37.0
48-49	36.50825412706353	37.0	37.0	37.0	37.0	37.0
50-51	36.39494747373686	37.0	37.0	37.0	37.0	37.0
52-53	36.45672836418209	37.0	37.0	37.0	37.0	37.0
54-55	36.493496748374184	37.0	37.0	37.0	37.0	37.0
56-57	36.48249124562281	37.0	37.0	37.0	37.0	37.0
58-59	36.449086815111336	37.0	37.0	37.0	37.0	37.0
60-61	36.503753753753756	37.0	37.0	37.0	37.0	37.0
62-63	36.35702197817342	37.0	37.0	37.0	37.0	37.0
64-65	36.3408425529408	37.0	37.0	37.0	37.0	37.0
66-67	36.41898322063611	37.0	37.0	37.0	37.0	37.0
68-69	36.35229066949326	37.0	37.0	37.0	37.0	37.0
70-71	36.378100726634926	37.0	37.0	37.0	37.0	37.0
72-73	36.36515388503551	37.0	37.0	37.0	37.0	37.0
74-75	36.325727181544636	37.0	37.0	37.0	37.0	37.0
76-77	36.32973921765296	37.0	37.0	37.0	37.0	37.0
78-79	36.383369111806346	37.0	37.0	37.0	37.0	37.0
80-81	36.27015608459593	37.0	37.0	37.0	37.0	37.0
82-83	36.342476094614995	37.0	37.0	37.0	37.0	37.0
84-85	36.39041204242342	37.0	37.0	37.0	37.0	37.0
86-87	36.39008972910071	37.0	37.0	37.0	37.0	37.0
88-89	36.34838197136398	37.0	37.0	37.0	37.0	37.0
90-91	36.35631122686483	37.0	37.0	37.0	37.0	37.0
92-93	36.233010888157196	37.0	37.0	37.0	37.0	37.0
94-95	36.22665461627666	37.0	37.0	37.0	37.0	37.0
96-97	36.16061720847476	37.0	37.0	37.0	37.0	37.0
98-99	36.24470466222429	37.0	37.0	37.0	37.0	37.0
100-101	36.507829781198396	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	2.0
27	4.0
28	14.0
29	19.0
30	19.0
31	29.0
32	48.0
33	65.0
34	78.0
35	193.0
36	2286.0
37	1242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.85	11.0	11.15	41.0
2	22.116587440580435	20.715536652489366	30.998248686514884	26.169627220415308
3	22.575	27.425	19.975	30.025000000000002
4	28.199999999999996	37.7	18.175	15.925
5	29.75	33.425	22.525000000000002	14.299999999999999
6	24.75	36.7	21.45	17.1
7	15.875	26.075	40.75	17.299999999999997
8	16.85	27.075	33.875	22.2
9	17.125	21.325	35.0	26.55
10-11	19.6375	36.2375	23.45	20.674999999999997
12-13	21.337500000000002	29.912499999999998	27.925	20.825
14-15	22.5625	23.724999999999998	26.25	27.462500000000002
16-17	22.6375	29.475	24.15	23.7375
18-19	22.1875	26.674999999999997	23.400000000000002	27.737499999999997
20-21	18.4125	29.049999999999997	26.700000000000003	25.837500000000002
22-23	22.95	27.762500000000003	26.5	22.787499999999998
24-25	24.212500000000002	26.437500000000004	29.525000000000002	19.825
26-27	23.625	26.5125	24.4375	25.424999999999997
28-29	22.95	26.2875	26.637499999999996	24.125
30-31	21.8875	28.249999999999996	25.4	24.462500000000002
32-33	18.4375	32.4625	22.237499999999997	26.8625
34-35	23.649324662331164	33.40420210105052	21.885942971485743	21.060530265132567
36-37	24.84992496248124	26.750875437718857	24.049524762381193	24.349674837418707
38-39	23.224112056028016	25.250125062531264	26.675837918959477	24.84992496248124
40-41	22.011005502751377	24.349674837418707	32.14107053526764	21.498249124562278
42-43	24.212106053026513	22.07353676838419	32.06603301650826	21.64832416208104
44-45	27.00100050025013	23.524262131065534	26.500750375187593	22.973986993496748
46-47	26.88844422211106	24.54977488744372	26.263131565782892	22.298649324662332
48-49	24.862431215607803	29.989994997498748	21.335667833916958	23.81190595297649
50-51	26.88844422211106	30.327663831915956	19.409704852426213	23.374187093546773
52-53	21.98599299649825	27.00100050025013	25.41270635317659	25.60030015007504
54-55	24.049524762381193	24.087043521760883	29.57728864432216	22.28614307153577
56-57	22.798899449724864	23.574287143571787	32.96648324162081	20.66033016508254
58-59	20.37778333750313	26.044533400050035	29.071803852889666	24.505879409557167
60-61	28.253253253253252	31.66916916916917	21.496496496496498	18.58108108108108
62-63	26.52984607683644	29.846076836440993	21.649355524965586	21.974721561756976
64-65	25.935661534610087	22.91901364376017	27.863312054074353	23.282012767555386
66-67	28.224392687202602	27.1099423991986	23.754069621838216	20.91159529176058
68-69	26.612398246712587	23.98246712586099	26.950532247964937	22.45460237946149
70-71	21.67376597344024	29.879729391130045	28.564269606614882	19.882235028814836
72-73	20.701754385964914	25.225563909774433	32.418546365914786	21.654135338345863
74-75	23.05667001003009	25.20060180541625	28.986960882647946	22.755767301905717
76-77	28.5481444332999	31.256268806419257	21.489468405215646	18.706118355065197
78-79	29.56314335927693	31.20763243786091	19.89706251569169	19.332161687170473
80-81	27.33098768534808	28.0849459663232	23.737119879366674	20.846946468962052
82-83	25.163563160543532	24.93709109209864	27.47861097131354	22.420734776044288
84-85	20.491803278688526	28.78940731399748	29.02900378310214	21.689785624211854
86-87	21.757797701730016	27.288799090794292	28.475817653744162	22.477585553731533
88-89	20.54239006463059	26.13103535673552	29.628690913699153	23.697883664934736
90-91	27.77636594663278	24.104193138500634	27.369758576874204	20.749682337992375
92-93	29.137469741368328	27.5321697031469	25.085998216333287	18.244362339151486
94-95	26.426195958045533	26.7587618316705	27.78204144282425	19.03300076745971
96-97	23.31579622738355	28.127807006287693	29.911459001668163	18.644937764660593
98-99	21.624173748819643	27.249426682854445	31.849453662484823	19.27694590584109
100-101	21.279734464318643	13.018624377650747	36.474276230868526	29.227364927162085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	0.0
24	0.0
25	0.5
26	0.5
27	3.0
28	3.5
29	0.5
30	2.5
31	4.5
32	5.0
33	11.5
34	17.5
35	18.5
36	26.0
37	45.5
38	67.0
39	108.5
40	149.0
41	183.0
42	160.0
43	178.0
44	318.5
45	306.0
46	366.0
47	410.0
48	327.5
49	264.0
50	169.5
51	155.5
52	133.5
53	126.0
54	91.0
55	67.0
56	108.5
57	76.0
58	24.0
59	31.5
60	32.5
61	15.5
62	4.0
63	2.0
64	2.0
65	1.5
66	1.5
67	2.0
68	1.5
69	1.0
70	1.0
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.075
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
32-33	2.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.0
58-59	1.0
60-61	0.0
62-63	1.0
64-65	2.0
66-67	0.0
68-69	2.0
70-71	0.0
72-73	3.0
74-75	0.0
76-77	4.0
78-79	4.0
80-81	6.0
82-83	7.0
84-85	5.0
86-87	15.0
88-89	9.0
90-91	10.0
92-93	18.0
94-95	8.0
96-97	50.0
98-99	645.0
100-101	3207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.299999999999997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.512367491166074	17.125
2	15.724381625441698	8.9
3	6.890459363957597	5.8500000000000005
4	3.6219081272084805	4.1000000000000005
5	1.9434628975265018	2.75
6	1.2367491166077738	2.1
7	0.441696113074205	0.8750000000000001
8	1.1484098939929328	2.6
9	1.2367491166077738	3.15
>10	6.5371024734982335	33.975
>50	0.35335689045936397	6.25
>100	0.35335689045936397	12.325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	131	3.2750000000000004	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	125	3.125	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	124	3.1	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	113	2.825	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	72	1.7999999999999998	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	69	1.725	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	58	1.4500000000000002	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	51	1.275	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	49	1.225	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	48	1.2	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	45	1.125	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	44	1.0999999999999999	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	43	1.075	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	41	1.0250000000000001	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	40	1.0	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	36	0.8999999999999999	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	36	0.8999999999999999	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	29	0.7250000000000001	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	27	0.675	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	25	0.625	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	24	0.6	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	24	0.6	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	23	0.575	No Hit
CTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGG	23	0.575	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	22	0.5499999999999999	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	22	0.5499999999999999	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	22	0.5499999999999999	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	22	0.5499999999999999	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	21	0.525	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	19	0.475	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	18	0.44999999999999996	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	18	0.44999999999999996	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	18	0.44999999999999996	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	17	0.42500000000000004	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	17	0.42500000000000004	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	17	0.42500000000000004	No Hit
TTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTC	17	0.42500000000000004	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	17	0.42500000000000004	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	16	0.4	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	16	0.4	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	16	0.4	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	16	0.4	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	15	0.375	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	14	0.35000000000000003	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	14	0.35000000000000003	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	14	0.35000000000000003	No Hit
CTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCC	13	0.325	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	13	0.325	No Hit
GGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	13	0.325	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	13	0.325	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	13	0.325	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	13	0.325	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	13	0.325	No Hit
CCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTA	13	0.325	No Hit
AGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTA	13	0.325	No Hit
CTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCG	13	0.325	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	13	0.325	No Hit
GTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATA	12	0.3	No Hit
GCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGC	12	0.3	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	12	0.3	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	12	0.3	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	11	0.27499999999999997	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	11	0.27499999999999997	No Hit
AGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAA	11	0.27499999999999997	No Hit
GGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGACCA	11	0.27499999999999997	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	11	0.27499999999999997	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	11	0.27499999999999997	No Hit
AGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTC	11	0.27499999999999997	No Hit
GTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGG	11	0.27499999999999997	No Hit
CTCCATTTGGCAGATGGTGAAACTGAGGCAGAGAATAAATTGCATGCCAA	11	0.27499999999999997	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	11	0.27499999999999997	No Hit
GTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGC	11	0.27499999999999997	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	11	0.27499999999999997	No Hit
TTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCT	11	0.27499999999999997	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	10	0.25	No Hit
TCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGG	10	0.25	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATC	10	0.25	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	10	0.25	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	10	0.25	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	10	0.25	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	10	0.25	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	10	0.25	No Hit
AGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTC	9	0.22499999999999998	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	9	0.22499999999999998	No Hit
ATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	9	0.22499999999999998	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	9	0.22499999999999998	No Hit
CAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTT	9	0.22499999999999998	No Hit
AGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGT	9	0.22499999999999998	No Hit
AGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTC	9	0.22499999999999998	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	9	0.22499999999999998	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	9	0.22499999999999998	No Hit
GCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTA	9	0.22499999999999998	No Hit
CGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTG	9	0.22499999999999998	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	9	0.22499999999999998	No Hit
TAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCA	9	0.22499999999999998	No Hit
GTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGA	9	0.22499999999999998	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCA	8	0.2	No Hit
TAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAA	8	0.2	No Hit
CCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGA	8	0.2	No Hit
TGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCT	8	0.2	No Hit
CTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGG	8	0.2	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	8	0.2	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	8	0.2	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	8	0.2	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	8	0.2	No Hit
GCATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTT	8	0.2	No Hit
GTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCA	8	0.2	No Hit
CCTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAG	8	0.2	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	8	0.2	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	7	0.17500000000000002	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	7	0.17500000000000002	No Hit
CTGAAATGAAGGAATCTCAATTAAGAAAATGCCTCTGTACAATCCAGCTG	7	0.17500000000000002	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	7	0.17500000000000002	No Hit
ATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAA	7	0.17500000000000002	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	6	0.15	No Hit
TGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTT	6	0.15	No Hit
CCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAA	6	0.15	No Hit
GGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGAC	6	0.15	No Hit
GTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGT	6	0.15	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	6	0.15	No Hit
ATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTGC	6	0.15	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	6	0.15	No Hit
CACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCC	6	0.15	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	6	0.15	No Hit
TGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAA	6	0.15	No Hit
CCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAG	6	0.15	No Hit
GACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGG	6	0.15	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	6	0.15	No Hit
GGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGA	5	0.125	No Hit
TGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAG	5	0.125	No Hit
TCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTC	5	0.125	No Hit
AGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAA	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
CATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTG	5	0.125	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	5	0.125	No Hit
CTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCG	5	0.125	No Hit
TTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGAC	5	0.125	No Hit
GTCAGATGTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGT	5	0.125	No Hit
TGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAG	5	0.125	No Hit
AGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTC	5	0.125	No Hit
CACACATGGAGGCCAGATGCAGGCACACATGGAGACCAGATGCAGGCACA	5	0.125	No Hit
TCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGC	5	0.125	No Hit
GGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCC	5	0.125	No Hit
TGGTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTC	5	0.125	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	5	0.125	No Hit
GGCCGATTTAATAAGTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCA	5	0.125	No Hit
ATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCA	5	0.125	No Hit
GTGGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGA	5	0.125	No Hit
AATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCC	5	0.125	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAGG	65	2.6172542E-5	30.493927	94-95
ATATCAG	65	2.6172542E-5	30.493927	94-95
CAAGAAT	95	2.879625E-5	29.73158	1
CCATGTG	95	2.879625E-5	29.73158	8
AAGAATC	95	2.879625E-5	29.73158	2
TCCATGT	95	2.879625E-5	29.73158	7
AGAATCC	100	4.0887375E-5	28.244999	3
CATGTGC	100	4.0887375E-5	28.244999	9
AATCCAT	105	5.704698E-5	26.9	5
ATCCATG	105	5.704698E-5	26.9	6
GAATCCA	105	5.704698E-5	26.9	4
TATTCAC	110	7.833842E-5	25.677273	5
CACTACC	110	7.833842E-5	25.677273	9
TCACTAC	110	7.833842E-5	25.677273	8
ATTCACT	110	7.833842E-5	25.677273	6
TTCACTA	115	1.0603021E-4	24.56087	7
AATATCA	65	1.3355915E-4	24.141026	92-93
CCATATT	105	0.0019380521	22.416666	2
TAATATC	70	2.2083474E-4	22.416666	92-93
GCCATAT	105	0.0019380521	22.416666	1
>>END_MODULE
SRR25656855 read2 length is 33-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656855_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	33-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2095	37.0	37.0	37.0	37.0	37.0
2	36.322	37.0	37.0	37.0	37.0	37.0
3	36.4415	37.0	37.0	37.0	37.0	37.0
4	36.4715	37.0	37.0	37.0	37.0	37.0
5	36.448	37.0	37.0	37.0	37.0	37.0
6	36.403	37.0	37.0	37.0	37.0	37.0
7	36.3865	37.0	37.0	37.0	37.0	37.0
8	36.466	37.0	37.0	37.0	37.0	37.0
9	36.4445	37.0	37.0	37.0	37.0	37.0
10-11	36.408249999999995	37.0	37.0	37.0	37.0	37.0
12-13	36.44925	37.0	37.0	37.0	37.0	37.0
14-15	36.328	37.0	37.0	37.0	37.0	37.0
16-17	36.3435	37.0	37.0	37.0	37.0	37.0
18-19	36.3925	37.0	37.0	37.0	37.0	37.0
20-21	36.451499999999996	37.0	37.0	37.0	37.0	37.0
22-23	36.411	37.0	37.0	37.0	37.0	37.0
24-25	36.4005	37.0	37.0	37.0	37.0	37.0
26-27	36.40275	37.0	37.0	37.0	37.0	37.0
28-29	36.323	37.0	37.0	37.0	37.0	37.0
30-31	36.311499999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.33125	37.0	37.0	37.0	37.0	37.0
34-35	36.21160580290145	37.0	37.0	37.0	37.0	37.0
36-37	36.24187093546773	37.0	37.0	37.0	37.0	37.0
38-39	36.24587293646823	37.0	37.0	37.0	37.0	37.0
40-41	36.322328727536146	37.0	37.0	37.0	37.0	37.0
42-43	36.04166262835265	37.0	37.0	37.0	37.0	37.0
44-45	36.27352352352352	37.0	37.0	37.0	37.0	37.0
46-47	36.13013013013013	37.0	37.0	37.0	37.0	37.0
48-49	36.18268268268268	37.0	37.0	37.0	37.0	37.0
50-51	36.18068068068068	37.0	37.0	37.0	37.0	37.0
52-53	36.0985985985986	37.0	37.0	37.0	37.0	37.0
54-55	36.17942942942943	37.0	37.0	37.0	37.0	37.0
56-57	36.120870870870874	37.0	37.0	37.0	37.0	37.0
58-59	36.14443053817271	37.0	37.0	37.0	37.0	37.0
60-61	36.16024036054081	37.0	37.0	37.0	37.0	37.0
62-63	36.06548095371206	37.0	37.0	37.0	37.0	37.0
64-65	36.08353201519493	37.0	37.0	37.0	37.0	37.0
66-67	36.101728890002505	37.0	37.0	37.0	37.0	37.0
68-69	36.04045524736421	37.0	37.0	37.0	37.0	37.0
70-71	36.14189019804462	37.0	37.0	37.0	37.0	37.0
72-73	36.01815071079278	37.0	37.0	37.0	37.0	37.0
74-75	35.99372804816859	37.0	37.0	37.0	37.0	37.0
76-77	36.12744606121425	37.0	37.0	37.0	37.0	37.0
78-79	35.961544033578235	37.0	37.0	37.0	37.0	37.0
80-81	36.0146044694512	37.0	37.0	37.0	37.0	37.0
82-83	36.11153071500503	37.0	37.0	37.0	37.0	37.0
84-85	35.983067633257804	37.0	37.0	37.0	37.0	37.0
86-87	35.97889865788096	37.0	37.0	37.0	37.0	37.0
88-89	35.91161177829373	37.0	37.0	37.0	37.0	37.0
90-91	36.05973035354674	37.0	37.0	37.0	37.0	37.0
92-93	36.09648910954829	37.0	37.0	37.0	37.0	37.0
94-95	36.10926881510795	37.0	37.0	37.0	37.0	37.0
96-97	36.02661923294063	37.0	37.0	37.0	37.0	37.0
98-99	36.01453036187939	37.0	37.0	37.0	37.0	37.0
100-101	36.24817669532753	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	3.0
25	4.0
26	9.0
27	8.0
28	16.0
29	15.0
30	29.0
31	37.0
32	47.0
33	70.0
34	124.0
35	381.0
36	2562.0
37	694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.325	17.724999999999998	14.85	31.1
2	24.4	23.65	28.7	23.25
3	20.75	28.549999999999997	28.050000000000004	22.650000000000002
4	22.900000000000002	36.6	22.825	17.675
5	25.374999999999996	35.85	20.25	18.525
6	22.475	32.074999999999996	24.95	20.5
7	18.099999999999998	22.1	37.4	22.400000000000002
8	20.375	21.875	29.45	28.299999999999997
9	22.6	25.75	25.724999999999998	25.924999999999997
10-11	22.8625	31.5125	21.9375	23.6875
12-13	20.175	26.775	27.737499999999997	25.3125
14-15	21.725	25.95	29.762499999999996	22.5625
16-17	20.5375	25.7125	27.2625	26.487500000000004
18-19	22.2625	26.450000000000003	27.6125	23.674999999999997
20-21	21.224999999999998	30.825000000000003	26.987499999999997	20.962500000000002
22-23	24.05	25.674999999999997	25.1875	25.087500000000002
24-25	19.575	26.8375	27.0125	26.575
26-27	20.8875	29.862499999999997	23.75	25.5
28-29	22.5625	27.35	24.1125	25.974999999999998
30-31	20.7125	24.087500000000002	29.225	25.974999999999998
32-33	21.85	25.9875	27.1625	25.0
34-35	25.76288144072036	25.30015007503752	24.61230615307654	24.324662331165584
36-37	24.92496248124062	28.27663831915958	27.063531765882942	19.734867433716857
38-39	24.437218609304654	26.32566283141571	26.475737868934466	22.761380690345174
40-41	18.273921200750472	27.954971857410882	27.879924953095685	25.89118198874296
42-43	23.54560240210184	27.298886525709996	23.72075566120355	25.43475541098461
44-45	23.54854854854855	27.314814814814813	25.850850850850847	23.285785785785787
46-47	22.54754754754755	27.13963963963964	26.163663663663662	24.14914914914915
48-49	22.42242242242242	23.84884884884885	29.52952952952953	24.1991991991992
50-51	18.615423134702052	27.25338007010516	26.4521782674011	27.679018527791687
52-53	21.846846846846844	29.89239239239239	26.076076076076077	22.184684684684687
54-55	19.694694694694697	27.52752752752753	28.27827827827828	24.4994994994995
56-57	20.12012012012012	28.678678678678676	25.663163163163162	25.538038038038035
58-59	21.73967459324155	25.844806007509387	30.175219023779725	22.24030037546934
60-61	22.8843264897346	27.341011517275916	24.036054081121684	25.7386079118678
62-63	19.782145987229246	26.893702266182544	28.721672718167024	24.602479028421183
64-65	23.807138384470882	25.835942391984972	26.762680025046965	23.59423919849718
66-67	23.415184164369833	26.998246053620644	23.352543222250063	26.23402655975946
68-69	20.674100989850896	27.12692645031951	24.934218769577747	27.26475379025185
70-71	23.163700175482578	25.582852845324645	26.49786914013537	24.75557783905741
72-73	24.557882854634393	26.11313181989214	24.47008654207952	24.858898783393954
74-75	17.837431008529855	27.87255393878575	29.691419969894632	24.598595082789764
76-77	23.99648770697441	25.1254390366282	26.116407425990968	24.761665830406425
78-79	20.96206983170058	24.830444611906557	27.216779703592064	26.990705852800804
80-81	22.743273824490824	29.356298717626352	26.175509177772188	21.724918280110636
82-83	23.85448136958711	26.120342396777442	25.08811681772407	24.93705941591138
84-85	19.934393136512742	27.84506686853394	27.857683573050718	24.3628564219026
86-87	23.46178142766898	29.39987365761213	25.2179406190777	21.920404295641188
88-89	22.612555485098287	31.426759670259983	23.62714013950539	22.333544705136337
90-91	20.879959308240082	26.004577822990843	25.940996948118006	27.17446592065107
92-93	22.810149177610608	24.760933316333038	26.2654596455438	26.16345786051256
94-95	22.510877911441003	28.20578448937804	25.287944714614795	23.995392884566165
96-97	23.057161207450225	29.903660886319845	25.279383429672446	21.75979447655748
98-99	24.86842105263158	25.32894736842105	26.0	23.80263157894737
100-101	19.926075268817204	11.35752688172043	32.39247311827957	36.32392473118279
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	1.5
29	2.0
30	7.0
31	10.5
32	6.5
33	10.0
34	14.0
35	18.5
36	21.5
37	72.0
38	105.0
39	91.0
40	130.5
41	148.5
42	151.5
43	188.5
44	207.5
45	193.0
46	293.0
47	445.0
48	398.5
49	255.0
50	184.5
51	196.5
52	218.5
53	239.5
54	179.0
55	66.5
56	41.5
57	33.0
58	15.0
59	16.0
60	22.0
61	16.0
62	9.0
63	6.5
64	6.0
65	5.0
66	1.5
67	1.5
68	1.5
69	1.0
70	1.0
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.050050050050050046
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.037612838515546636
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
32-33	2.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.0
42-43	1.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.0
58-59	1.0
60-61	0.0
62-63	1.0
64-65	2.0
66-67	0.0
68-69	2.0
70-71	0.0
72-73	3.0
74-75	0.0
76-77	4.0
78-79	4.0
80-81	6.0
82-83	7.0
84-85	5.0
86-87	16.0
88-89	9.0
90-91	10.0
92-93	17.0
94-95	10.0
96-97	57.0
98-99	347.0
100-101	3494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.599999999999998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.2195945945946	17.825
2	14.442567567567568	8.55
3	5.489864864864865	4.875
4	3.6317567567567566	4.3
5	2.5337837837837838	3.75
6	2.2804054054054053	4.05
7	0.9290540540540541	1.925
8	1.9425675675675675	4.6
9	1.097972972972973	2.9250000000000003
>10	7.094594594594595	40.875
>50	0.33783783783783783	6.325
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	71	1.775	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	70	1.7500000000000002	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	61	1.525	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	51	1.275	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	50	1.25	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	49	1.225	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	48	1.2	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	43	1.075	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	42	1.05	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	41	1.0250000000000001	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	39	0.975	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	39	0.975	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	39	0.975	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	37	0.9249999999999999	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	36	0.8999999999999999	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	35	0.8750000000000001	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	33	0.8250000000000001	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	33	0.8250000000000001	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	31	0.775	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	28	0.7000000000000001	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	27	0.675	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	26	0.65	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	25	0.625	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	25	0.625	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	22	0.5499999999999999	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	21	0.525	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	21	0.525	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	21	0.525	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	20	0.5	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	20	0.5	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	20	0.5	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	20	0.5	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	19	0.475	No Hit
GCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGG	19	0.475	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	19	0.475	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	19	0.475	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	18	0.44999999999999996	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	18	0.44999999999999996	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	17	0.42500000000000004	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	17	0.42500000000000004	No Hit
GCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCA	17	0.42500000000000004	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	17	0.42500000000000004	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	17	0.42500000000000004	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	17	0.42500000000000004	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	16	0.4	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	16	0.4	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	15	0.375	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	15	0.375	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	15	0.375	No Hit
AGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTC	15	0.375	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	14	0.35000000000000003	No Hit
TGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCT	14	0.35000000000000003	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	14	0.35000000000000003	No Hit
GGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	14	0.35000000000000003	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	14	0.35000000000000003	No Hit
GCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTG	13	0.325	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	13	0.325	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	13	0.325	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	13	0.325	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	12	0.3	No Hit
TGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCA	12	0.3	No Hit
TGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATA	12	0.3	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	12	0.3	No Hit
CTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAA	12	0.3	No Hit
TAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTC	12	0.3	No Hit
TGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCT	12	0.3	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	12	0.3	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	11	0.27499999999999997	No Hit
GCCCGACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGGA	11	0.27499999999999997	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	11	0.27499999999999997	No Hit
TTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAA	11	0.27499999999999997	No Hit
ATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAG	11	0.27499999999999997	No Hit
CAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCT	11	0.27499999999999997	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	11	0.27499999999999997	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	11	0.27499999999999997	No Hit
GGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTG	11	0.27499999999999997	No Hit
ACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATG	11	0.27499999999999997	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	10	0.25	No Hit
CCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAA	10	0.25	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	10	0.25	No Hit
GCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTA	10	0.25	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	10	0.25	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGT	10	0.25	No Hit
GGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATA	10	0.25	No Hit
GCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGG	10	0.25	No Hit
AGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTT	10	0.25	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	10	0.25	No Hit
CCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCT	10	0.25	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	9	0.22499999999999998	No Hit
CCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTA	9	0.22499999999999998	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGCA	9	0.22499999999999998	No Hit
AGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTG	9	0.22499999999999998	No Hit
CTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGG	9	0.22499999999999998	No Hit
GAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTG	9	0.22499999999999998	No Hit
TGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTC	9	0.22499999999999998	No Hit
TTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAA	9	0.22499999999999998	No Hit
CGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCA	9	0.22499999999999998	No Hit
GCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTG	9	0.22499999999999998	No Hit
ATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGA	9	0.22499999999999998	No Hit
GCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAG	9	0.22499999999999998	No Hit
CCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAAC	9	0.22499999999999998	No Hit
CGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCT	8	0.2	No Hit
CGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACT	8	0.2	No Hit
GTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCA	8	0.2	No Hit
CTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCC	8	0.2	No Hit
AATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACC	8	0.2	No Hit
AGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCT	8	0.2	No Hit
ATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTG	8	0.2	No Hit
CTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCG	8	0.2	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	8	0.2	No Hit
GGATTTTCGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTG	8	0.2	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	8	0.2	No Hit
TGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATG	8	0.2	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	8	0.2	No Hit
CCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGA	8	0.2	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	8	0.2	No Hit
CGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCC	8	0.2	No Hit
GGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCT	8	0.2	No Hit
ATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGA	8	0.2	No Hit
CTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTA	8	0.2	No Hit
CTTCTGACTTCCATGGATTTTCGGCAAGATACTCAATACACATAAAATAA	8	0.2	No Hit
AGAAAATGAACTGATGTATGGAGTTGCATTTGAACTTCCTGCAGAAGCTC	8	0.2	No Hit
TTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAAT	8	0.2	No Hit
AGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCC	8	0.2	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	7	0.17500000000000002	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	7	0.17500000000000002	No Hit
CAAGAAGTCTAGGGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCG	7	0.17500000000000002	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	7	0.17500000000000002	No Hit
GTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAA	7	0.17500000000000002	No Hit
TACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCC	7	0.17500000000000002	No Hit
TATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGA	7	0.17500000000000002	No Hit
GGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATC	7	0.17500000000000002	No Hit
ATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTT	7	0.17500000000000002	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	7	0.17500000000000002	No Hit
GCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	7	0.17500000000000002	No Hit
AAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAA	6	0.15	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	6	0.15	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGA	6	0.15	No Hit
GAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAG	6	0.15	No Hit
TCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATG	6	0.15	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	6	0.15	No Hit
TTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCT	6	0.15	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	6	0.15	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	6	0.15	No Hit
CTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	6	0.15	No Hit
GACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGG	6	0.15	No Hit
AACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGA	6	0.15	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	6	0.15	No Hit
CCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGA	6	0.15	No Hit
GAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAA	6	0.15	No Hit
AGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTGGA	6	0.15	No Hit
AAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCG	6	0.15	No Hit
ACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCA	6	0.15	No Hit
CTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCAC	6	0.15	No Hit
GGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGT	6	0.15	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	6	0.15	No Hit
ATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAG	6	0.15	No Hit
ATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAA	6	0.15	No Hit
TCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGT	6	0.15	No Hit
TGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCA	6	0.15	No Hit
GCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATA	6	0.15	No Hit
TTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGAT	6	0.15	No Hit
GAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATAT	5	0.125	No Hit
GCAGCTATGGCTGGGTTGCGGCCCATCTGTGAATTTATGACCTTCAATTT	5	0.125	No Hit
CAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTAC	5	0.125	No Hit
AATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAA	5	0.125	No Hit
TTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGG	5	0.125	No Hit
AAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAA	5	0.125	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	5	0.125	No Hit
GCTATGGCTGGGTTGCGGCCCATCTGTGAATTTATGACCTTCAATTTCTC	5	0.125	No Hit
AGCAAAAGCTGCACGAGCGACTGAGCCTTTGAGGGTGTGTGCTTGAAAAT	5	0.125	No Hit
ACTCAATACACATAAAATAATAAAAAAGCCATTGAGAAAAGACATCTGAC	5	0.125	No Hit
AAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTGG	5	0.125	No Hit
GAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCA	5	0.125	No Hit
AAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGA	5	0.125	No Hit
ATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTC	5	0.125	No Hit
GATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGA	5	0.125	No Hit
ATACAAGGTTAGCAGAGGCCTGTGGAAGAAATACGGTGACAAGAGGATCA	5	0.125	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	5	0.125	No Hit
TATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCT	5	0.125	No Hit
TCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAA	5	0.125	No Hit
AAAAAAGCCATTGAGAAAAGACATCTGACCAGCATCTGGCCTCCATGTGT	5	0.125	No Hit
CTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGC	5	0.125	No Hit
CTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGT	5	0.125	No Hit
ATTTTCGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTGAG	5	0.125	No Hit
CTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTG	5	0.125	No Hit
TTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTC	5	0.125	No Hit
CTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAG	5	0.125	No Hit
GAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCT	5	0.125	No Hit
TACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTC	5	0.125	No Hit
AGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATG	5	0.125	No Hit
ATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
Read 19792655 spots for SRR25656855.sra
Written 19792655 spots for SRR25656855.sra
SRR ids: ['SRR25656855.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9kgofn90
SRR25656855.sra spots: 158341240
blocks: [[1, 19792655], [19792656, 39585310], [39585311, 59377965], [59377966, 79170620], [79170621, 98963275], [98963276, 118755930], [118755931, 138548585], [138548586, 158341240]]
SRR25656855 file size 38162008
SRR25656855 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25656855 SRR25656855_1.fastq SRR25656855_2.fastq
Input file:	SRR25656855_1.fastq
Paired file:	SRR25656855_2.fastq
trimmed:	SRR25656855-trimmed-pair1.fastq, SRR25656855-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 17:39:13 2026 >> started

Thu May 21 17:46:54 2026 >> done (461.001s)
158341240 read pairs processed; of these:
        0 ( 0.00%) short read pairs filtered out after trimming by size control
      413 ( 0.00%) empty read pairs filtered out after trimming by size control
158340827 (100.00%) read pairs available; of these:
    38840 ( 0.02%) trimmed read pairs available after processing
158301987 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	       44	  0.00%
 21	       50	  0.00%
 22	       70	  0.00%
 23	       55	  0.00%
 24	       61	  0.00%
 25	      108	  0.00%
 26	      124	  0.00%
 27	      139	  0.00%
 28	      183	  0.00%
 29	      240	  0.00%
 30	      263	  0.00%
 31	      333	  0.00%
 32	      372	  0.00%
 33	      439	  0.00%
 34	      527	  0.00%
 35	      512	  0.00%
 36	      586	  0.00%
 37	      772	  0.00%
 38	      897	  0.00%
 39	     1029	  0.00%
 40	     1212	  0.00%
 41	     1539	  0.00%
 42	     1626	  0.00%
 43	     1676	  0.00%
 44	     1809	  0.00%
 45	     1943	  0.00%
 46	     2272	  0.00%
 47	     2838	  0.00%
 48	     3182	  0.00%
 49	     3998	  0.00%
 50	     4251	  0.00%
 51	     4600	  0.00%
 52	     5128	  0.00%
 53	     5585	  0.00%
 54	     6014	  0.00%
 55	     6421	  0.00%
 56	     6794	  0.00%
 57	     7674	  0.00%
 58	     8964	  0.01%
 59	    14366	  0.01%
 60	    57373	  0.04%
 61	    75061	  0.05%
 62	    59017	  0.04%
 63	    58229	  0.04%
 64	    47171	  0.03%
 65	    49475	  0.03%
 66	    44913	  0.03%
 67	    52824	  0.03%
 68	    42640	  0.03%
 69	    47030	  0.03%
 70	    58081	  0.04%
 71	    54212	  0.03%
 72	    46618	  0.03%
 73	    48465	  0.03%
 74	    50729	  0.03%
 75	    55098	  0.03%
 76	    60787	  0.04%
 77	    64974	  0.04%
 78	    68698	  0.04%
 79	    76472	  0.05%
 80	    86447	  0.05%
 81	    95208	  0.06%
 82	   106970	  0.07%
 83	   115716	  0.07%
 84	   122693	  0.08%
 85	   137202	  0.09%
 86	   151879	  0.10%
 87	   162151	  0.10%
 88	   169606	  0.11%
 89	   185470	  0.12%
 90	   208209	  0.13%
 91	   232681	  0.15%
 92	   251934	  0.16%
 93	   282390	  0.18%
 94	   320837	  0.20%
 95	   355570	  0.22%
 96	   423194	  0.27%
 97	   566837	  0.36%
 98	  1967833	  1.24%
 99	 13831914	  8.74%
100	 70771109	 44.70%
101	 66578414	 42.05%
158340827 reads passed initial QC


criterion=sequence-density
sequence-density=0.90
sequence-density-rank=1
fanout-score=4.89
fanout-score-rank=16
prefix-density=1.22
prefix-fanout=3.6
sequence=CCAGGAGAACCA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=42.41
fanout-score-rank=1
prefix-density=0.44
prefix-fanout=1.1
sequence=TTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTA


criterion=sequence-density
sequence-density=2.46
sequence-density-rank=1
fanout-score=7.44
fanout-score-rank=8
prefix-density=5.08
prefix-fanout=3.6
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=0.62
sequence-density-rank=20
fanout-score=17.54
fanout-score-rank=1
prefix-density=1.85
prefix-fanout=5.9
sequence=CCAGGTCCTGCAGG
SRR25656855 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 17:47:13
                             Started mapping on |	May 21 17:47:13
                                    Finished on |	May 21 17:56:53
       Mapping speed, Million of reads per hour |	982.81

                          Number of input reads |	158340827
                      Average input read length |	200
                                    UNIQUE READS:
                   Uniquely mapped reads number |	131753392
                        Uniquely mapped reads % |	83.21%
                          Average mapped length |	199.55
                       Number of splices: Total |	100874581
            Number of splices: Annotated (sjdb) |	100316296
                       Number of splices: GT/AG |	99666593
                       Number of splices: GC/AG |	1009749
                       Number of splices: AT/AC |	102766
               Number of splices: Non-canonical |	95473
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.58
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.59
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	17853702
             % of reads mapped to multiple loci |	11.28%
        Number of reads mapped to too many loci |	849900
             % of reads mapped to too many loci |	0.54%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.91%
                     % of reads unmapped: other |	0.07%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9109533	9109533	9109533
N_multimapping	17853702	17853702	17853702
N_noFeature	8094022	125843239	8627217
N_ambiguous	7756857	65070	2520377
UnstrandedReadsAssigned:115902513 PositiveStrandReadsAssigned:5845083 NegativeStrandReadsAssigned:120605798
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=100 echo kmer=95
SRR25656855 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25656855-trimmed-pair1.fastq
                             SRR25656855-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 158,340,827 reads, 124,002,939 reads pseudoaligned
[quant] estimated average fragment length: 195.003
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,324 rounds

 109283 SRR25656855.ke.tsv
  52636 SRR25656855.se.tsv
 161919 total
==> SRR25656855.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	10	0	0
ENSMUST00000196221.1	9	3	0	0
ENSMUST00000179664.1	11	5	0	0
ENSMUST00000178537.1	12	6	0	0
ENSMUST00000178862.1	14	8	0	0
ENSMUST00000179520.1	11	5	0	0
ENSMUST00000179883.1	16	10	0	0
ENSMUST00000195858.1	10	4	0	0
ENSMUST00000179932.1	12	6	0	0

==> SRR25656855.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	6
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25656855 completed mapping pipeline successfully
