Starting /dee2/code/volunteer_pipeline.sh SRR25656851
    current disk space = 1105348576
    free memory = 193798240 
SRR25656851 SRAfilesize
0585ecc7f35db5def3ceecf0f200b3a5  SRR25656851.sra
SRR25656851.sra file validated
SRR25656851 is paired end
SRR25656851 is conventional basespace
SRR25656851 read1 length is 35-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.582	37.0	37.0	37.0	37.0	37.0
2	36.4835	37.0	37.0	37.0	37.0	37.0
3	36.608	37.0	37.0	37.0	37.0	37.0
4	36.60625	37.0	37.0	37.0	37.0	37.0
5	36.6315	37.0	37.0	37.0	37.0	37.0
6	36.6865	37.0	37.0	37.0	37.0	37.0
7	36.5035	37.0	37.0	37.0	37.0	37.0
8	36.617	37.0	37.0	37.0	37.0	37.0
9	36.593	37.0	37.0	37.0	37.0	37.0
10-11	36.613749999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.595749999999995	37.0	37.0	37.0	37.0	37.0
14-15	36.60525	37.0	37.0	37.0	37.0	37.0
16-17	36.5925	37.0	37.0	37.0	37.0	37.0
18-19	36.5835	37.0	37.0	37.0	37.0	37.0
20-21	36.5945	37.0	37.0	37.0	37.0	37.0
22-23	36.621750000000006	37.0	37.0	37.0	37.0	37.0
24-25	36.52875	37.0	37.0	37.0	37.0	37.0
26-27	36.52475	37.0	37.0	37.0	37.0	37.0
28-29	36.56875	37.0	37.0	37.0	37.0	37.0
30-31	36.549	37.0	37.0	37.0	37.0	37.0
32-33	36.50675	37.0	37.0	37.0	37.0	37.0
34-35	36.5825	37.0	37.0	37.0	37.0	37.0
36-37	36.54663665916479	37.0	37.0	37.0	37.0	37.0
38-39	36.498374593648414	37.0	37.0	37.0	37.0	37.0
40-41	36.477869467366844	37.0	37.0	37.0	37.0	37.0
42-43	36.47311827956989	37.0	37.0	37.0	37.0	37.0
44-45	36.44036009002251	37.0	37.0	37.0	37.0	37.0
46-47	36.396849212303074	37.0	37.0	37.0	37.0	37.0
48-49	36.413206603301646	37.0	37.0	37.0	37.0	37.0
50-51	36.380190095047524	37.0	37.0	37.0	37.0	37.0
52-53	36.374937468734366	37.0	37.0	37.0	37.0	37.0
54-55	36.437968984492244	37.0	37.0	37.0	37.0	37.0
56-57	36.40095047523762	37.0	37.0	37.0	37.0	37.0
58-59	36.44297148574287	37.0	37.0	37.0	37.0	37.0
60-61	36.445963321051494	37.0	37.0	37.0	37.0	37.0
62-63	36.38733099649474	37.0	37.0	37.0	37.0	37.0
64-65	36.393340010015024	37.0	37.0	37.0	37.0	37.0
66-67	36.429644466700054	37.0	37.0	37.0	37.0	37.0
68-69	36.27395965822902	37.0	37.0	37.0	37.0	37.0
70-71	36.30834795688142	37.0	37.0	37.0	37.0	37.0
72-73	36.26472800200551	37.0	37.0	37.0	37.0	37.0
74-75	36.367960366673614	37.0	37.0	37.0	37.0	37.0
76-77	36.27065026362038	37.0	37.0	37.0	37.0	37.0
78-79	36.233851578333656	37.0	37.0	37.0	37.0	37.0
80-81	36.210963037465426	37.0	37.0	37.0	37.0	37.0
82-83	36.23949699708062	37.0	37.0	37.0	37.0	37.0
84-85	36.297288822300104	37.0	37.0	37.0	37.0	37.0
86-87	36.275951995090374	37.0	37.0	37.0	37.0	37.0
88-89	36.29339007814397	37.0	37.0	37.0	37.0	37.0
90-91	36.21992485863377	37.0	37.0	37.0	37.0	37.0
92-93	36.203704718594565	37.0	37.0	37.0	37.0	37.0
94-95	36.115850412458514	37.0	37.0	37.0	37.0	37.0
96-97	36.10081712233085	37.0	37.0	37.0	37.0	37.0
98-99	36.27557299045131	37.0	37.0	37.0	37.0	37.0
100-101	36.39303696477144	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	1.0
26	4.0
27	5.0
28	10.0
29	13.0
30	21.0
31	30.0
32	39.0
33	53.0
34	98.0
35	217.0
36	2446.0
37	1061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.9	10.85	11.975	39.275
2	23.736868434217108	19.384692346173086	30.340170085042523	26.538269134567283
3	22.725	26.025	21.425	29.825000000000003
4	27.206801700425103	35.13378344586147	21.05526381595399	16.60415103775944
5	30.625000000000004	31.35	23.825	14.2
6	24.625	36.825	20.775	17.775
7	15.950000000000001	26.724999999999998	40.550000000000004	16.775000000000002
8	17.56756756756757	27.902902902902905	31.631631631631627	22.8978978978979
9	18.8	24.55	34.449999999999996	22.2
10-11	21.2375	37.525	22.237499999999997	19.0
12-13	21.6625	28.012500000000003	29.4375	20.8875
14-15	22.4875	25.4875	25.7375	26.2875
16-17	22.75	29.2375	24.224999999999998	23.7875
18-19	22.55	26.8125	25.374999999999996	25.2625
20-21	20.474999999999998	27.725	28.499999999999996	23.3
22-23	22.25	28.050000000000004	28.075	21.625
24-25	23.0375	26.237500000000004	31.1875	19.537499999999998
26-27	22.8875	27.487499999999997	25.775	23.849999999999998
28-29	23.5625	26.325	26.087500000000002	24.025
30-31	22.575	27.4125	26.8	23.2125
32-33	20.0375	31.175000000000004	25.25	23.5375
34-35	24.2375	30.837500000000002	24.65	20.275000000000002
36-37	23.543385846461614	27.369342335583895	25.468867216804203	23.618404601150285
38-39	23.793448362090523	24.48112028007002	28.782195548887223	22.943235808952238
40-41	21.517879469867466	23.43085771442861	31.957989497374346	23.093273318329583
42-43	25.79394848712178	21.94298574643661	30.882720680170046	21.380345086271568
44-45	25.35633908477119	22.943235808952238	28.232058014503625	23.468367091772944
46-47	25.906476619154787	25.6064016004001	25.28132033008252	23.20580145036259
48-49	25.57528764382191	29.864932466233117	21.64832416208104	22.911455727863935
50-51	24.61230615307654	30.6528264132066	19.78489244622311	24.949974987493746
52-53	20.810405202601302	27.851425712856425	27.226113056528263	24.112056028014006
54-55	23.949474737368686	25.0	29.214607303651825	21.83591795897949
56-57	23.88694347173587	24.249624812406203	31.21560780390195	20.647823911955978
58-59	21.46073036518259	26.93846923461731	28.68934467233617	22.911455727863935
60-61	25.68818818818819	29.116616616616614	25.93843843843844	19.256756756756758
62-63	25.237856785177765	30.15773660490736	23.322483725588384	21.28192288432649
64-65	24.837255883825737	24.499248873309966	29.644466700050074	21.019028542814223
66-67	25.701051577366048	28.680520781171758	24.72458688032048	20.893840761141714
68-69	24.26406112990104	26.493799323562573	26.568959037955658	22.673180508580735
70-71	21.38380546502883	30.007520681875157	28.666332414138886	19.942341438957133
72-73	21.3712709952369	26.873903233893202	31.02281273502131	20.732013035848585
74-75	22.042403713461297	26.282775059591017	27.963869025216407	23.710952201731274
76-77	27.454180266131058	29.814210394175245	23.022847100175746	19.708762239517952
78-79	27.957799547852296	29.716151720673196	22.218035669429792	20.108013062044712
80-81	25.660045260246417	27.33215991953734	26.15036459642947	20.857430223786775
82-83	24.0	25.660377358490567	28.842767295597483	21.49685534591195
84-85	19.243856332703213	28.27977315689981	32.96786389413989	19.508506616257087
86-87	20.41640378548896	27.305993690851736	30.031545741324923	22.246056782334385
88-89	20.468354430379748	26.101265822784807	29.759493670886076	23.67088607594937
90-91	26.34989200863931	25.155634608054882	27.899885656206326	20.59458772709948
92-93	28.276389597144313	27.12901580826109	24.910759816420196	19.6838347781744
94-95	25.61771860197158	26.872359493022664	27.20522340289336	20.304698502112405
96-97	22.756492671637954	30.23913602468501	28.156338390331705	18.84803291334533
98-99	22.173097534833868	27.773311897106108	31.095927116827436	18.957663451232584
100-101	21.78680568038828	13.266223260830486	35.969800467373716	28.977170591407514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	1.0
14	1.0
15	0.0
16	1.5
17	1.5
18	0.0
19	1.0
20	1.0
21	0.5
22	0.5
23	0.5
24	1.5
25	1.5
26	3.0
27	4.5
28	6.5
29	10.5
30	8.5
31	8.0
32	11.0
33	16.0
34	27.5
35	32.5
36	35.5
37	51.0
38	82.0
39	140.5
40	152.5
41	168.0
42	148.0
43	187.0
44	362.0
45	337.5
46	377.5
47	395.5
48	282.5
49	223.0
50	145.0
51	143.5
52	137.5
53	131.5
54	99.5
55	67.5
56	82.0
57	54.0
58	16.5
59	14.5
60	21.0
61	15.5
62	5.5
63	3.0
64	0.5
65	0.0
66	0.0
67	0.0
68	0.5
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05
3	0.0
4	0.025
5	0.0
6	0.0
7	0.0
8	0.1
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	1.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.0
60-61	3.0
62-63	0.0
64-65	0.0
66-67	2.0
68-69	3.0
70-71	0.0
72-73	2.0
74-75	4.0
76-77	1.0
78-79	5.0
80-81	1.0
82-83	8.0
84-85	2.0
86-87	12.0
88-89	14.0
90-91	13.0
92-93	20.0
94-95	13.0
96-97	46.0
98-99	579.0
100-101	3269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.150000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.90922401171303	21.825
2	14.494875549048317	9.9
3	6.222547584187408	6.375
4	3.1478770131771596	4.3
5	1.6837481698389458	2.875
6	2.049780380673499	4.2
7	1.0248901903367496	2.45
8	1.2445095168374818	3.4000000000000004
9	0.8784773060029283	2.7
>10	4.978038067349927	32.275
>50	0.29282576866764276	6.825
>100	0.07320644216691069	2.875
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	115	2.875	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	76	1.9	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	73	1.825	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	71	1.775	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	53	1.325	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	49	1.225	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	47	1.175	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	44	1.0999999999999999	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	43	1.075	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	42	1.05	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	42	1.05	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	37	0.9249999999999999	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	37	0.9249999999999999	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	33	0.8250000000000001	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	28	0.7000000000000001	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	28	0.7000000000000001	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	26	0.65	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	26	0.65	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	24	0.6	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	23	0.575	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	22	0.5499999999999999	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	21	0.525	No Hit
CCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGA	20	0.5	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	20	0.5	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	20	0.5	No Hit
CTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCG	20	0.5	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	19	0.475	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	19	0.475	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	18	0.44999999999999996	No Hit
CCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGT	17	0.42500000000000004	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	17	0.42500000000000004	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	17	0.42500000000000004	No Hit
CCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTA	17	0.42500000000000004	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	17	0.42500000000000004	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	17	0.42500000000000004	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	16	0.4	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	16	0.4	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	16	0.4	No Hit
GTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGA	16	0.4	No Hit
GCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACA	15	0.375	No Hit
GTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAA	15	0.375	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	15	0.375	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	15	0.375	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	15	0.375	No Hit
GTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGA	14	0.35000000000000003	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	14	0.35000000000000003	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	14	0.35000000000000003	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	13	0.325	No Hit
CTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCC	13	0.325	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATC	13	0.325	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	13	0.325	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	12	0.3	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	12	0.3	No Hit
CCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGG	12	0.3	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	12	0.3	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	12	0.3	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGC	12	0.3	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	12	0.3	No Hit
TTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTC	12	0.3	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	12	0.3	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	12	0.3	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	11	0.27499999999999997	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	11	0.27499999999999997	No Hit
CACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACC	11	0.27499999999999997	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	11	0.27499999999999997	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	11	0.27499999999999997	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	11	0.27499999999999997	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	11	0.27499999999999997	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	11	0.27499999999999997	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	10	0.25	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	10	0.25	No Hit
CATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGAT	10	0.25	No Hit
TGAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCC	10	0.25	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	9	0.22499999999999998	No Hit
GGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	9	0.22499999999999998	No Hit
GTTTGTTTGAACTTTATTTTTATTTTGTTTTTTAGTCTATGTGTATGTGT	9	0.22499999999999998	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	9	0.22499999999999998	No Hit
GTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGG	9	0.22499999999999998	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	9	0.22499999999999998	No Hit
AGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTC	9	0.22499999999999998	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	9	0.22499999999999998	No Hit
GGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATT	9	0.22499999999999998	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	9	0.22499999999999998	No Hit
GGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAAT	9	0.22499999999999998	No Hit
CGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTG	9	0.22499999999999998	No Hit
GTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTG	8	0.2	No Hit
AGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAA	8	0.2	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	8	0.2	No Hit
AGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACT	8	0.2	No Hit
ATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	8	0.2	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	8	0.2	No Hit
GGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCG	8	0.2	No Hit
GTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTT	8	0.2	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	8	0.2	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	8	0.2	No Hit
CTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGG	8	0.2	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	8	0.2	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	8	0.2	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	8	0.2	No Hit
GTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCA	8	0.2	No Hit
CGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTT	8	0.2	No Hit
GGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAG	8	0.2	No Hit
GCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCCCTAGACTTCT	7	0.17500000000000002	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	7	0.17500000000000002	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	7	0.17500000000000002	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	7	0.17500000000000002	No Hit
GGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGT	7	0.17500000000000002	No Hit
GTCAGATGTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGT	7	0.17500000000000002	No Hit
TGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCT	7	0.17500000000000002	No Hit
TCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGC	7	0.17500000000000002	No Hit
GGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCC	7	0.17500000000000002	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	7	0.17500000000000002	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	7	0.17500000000000002	No Hit
CCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCAC	7	0.17500000000000002	No Hit
GGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAG	7	0.17500000000000002	No Hit
TGAGCAGTGAAGGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAAT	7	0.17500000000000002	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	6	0.15	No Hit
TTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGT	6	0.15	No Hit
GTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATA	6	0.15	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	6	0.15	No Hit
GTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAG	6	0.15	No Hit
AGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGTC	6	0.15	No Hit
AGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAA	6	0.15	No Hit
GGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGACCA	6	0.15	No Hit
GCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTAT	6	0.15	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	6	0.15	No Hit
TTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGAC	6	0.15	No Hit
GCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCT	6	0.15	No Hit
ATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGG	6	0.15	No Hit
CTCCATTTGGCAGATGGTGAAACTGAGGCAGAGAATAAATTGCATGCCAA	6	0.15	No Hit
TCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTT	6	0.15	No Hit
GTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGT	6	0.15	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	6	0.15	No Hit
TCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTG	6	0.15	No Hit
GGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCAT	6	0.15	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	6	0.15	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	6	0.15	No Hit
GTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGC	6	0.15	No Hit
AGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTA	6	0.15	No Hit
GCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTA	6	0.15	No Hit
ATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAA	6	0.15	No Hit
GCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCA	6	0.15	No Hit
GTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTC	6	0.15	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	6	0.15	No Hit
ACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCG	5	0.125	No Hit
GGGATGTGGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACAC	5	0.125	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	5	0.125	No Hit
GGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCA	5	0.125	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	5	0.125	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	5	0.125	No Hit
CTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCCCTAGACT	5	0.125	No Hit
GTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGC	5	0.125	No Hit
AGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTC	5	0.125	No Hit
AAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGG	5	0.125	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	5	0.125	No Hit
CTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGC	5	0.125	No Hit
AGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAA	5	0.125	No Hit
TTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAA	5	0.125	No Hit
ATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTGC	5	0.125	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	5	0.125	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	5	0.125	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	5	0.125	No Hit
GTGGGACTGTAGTTCCAGTTTTGAGTACCATCATGTGGGACTGTAGTTCC	5	0.125	No Hit
TTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCT	5	0.125	No Hit
GAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTA	5	0.125	No Hit
GCCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGT	5	0.125	No Hit
CTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACCC	50	0.0016038261	23.51875	10-11
TACCCAC	50	0.0016038261	23.51875	12-13
CCCACAA	55	0.0027920085	21.38068	14-15
CACAAGA	60	0.0046243775	19.598959	16-17
>>END_MODULE
SRR25656851 read2 length is 35-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656851_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	35-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.376	37.0	37.0	37.0	37.0	37.0
2	36.246	37.0	37.0	37.0	37.0	37.0
3	36.3155	37.0	37.0	37.0	37.0	37.0
4	36.4225	37.0	37.0	37.0	37.0	37.0
5	36.29	37.0	37.0	37.0	37.0	37.0
6	36.205	37.0	37.0	37.0	37.0	37.0
7	36.224	37.0	37.0	37.0	37.0	37.0
8	36.4455	37.0	37.0	37.0	37.0	37.0
9	36.3635	37.0	37.0	37.0	37.0	37.0
10-11	36.381	37.0	37.0	37.0	37.0	37.0
12-13	36.3485	37.0	37.0	37.0	37.0	37.0
14-15	36.1935	37.0	37.0	37.0	37.0	37.0
16-17	36.3435	37.0	37.0	37.0	37.0	37.0
18-19	36.28125	37.0	37.0	37.0	37.0	37.0
20-21	36.337	37.0	37.0	37.0	37.0	37.0
22-23	36.34375	37.0	37.0	37.0	37.0	37.0
24-25	36.252250000000004	37.0	37.0	37.0	37.0	37.0
26-27	36.171499999999995	37.0	37.0	37.0	37.0	37.0
28-29	36.19	37.0	37.0	37.0	37.0	37.0
30-31	36.08775	37.0	37.0	37.0	37.0	37.0
32-33	36.1065	37.0	37.0	37.0	37.0	37.0
34-35	36.14725	37.0	37.0	37.0	37.0	37.0
36-37	36.07565398102902	37.0	37.0	37.0	37.0	37.0
38-39	36.07228614307154	37.0	37.0	37.0	37.0	37.0
40-41	36.11380690345173	37.0	37.0	37.0	37.0	37.0
42-43	36.02601300650325	37.0	37.0	37.0	37.0	37.0
44-45	35.951225612806404	37.0	37.0	37.0	37.0	37.0
46-47	35.95897948974488	37.0	37.0	37.0	37.0	37.0
48-49	36.01401050788091	37.0	37.0	37.0	37.0	37.0
50-51	35.979354354354356	37.0	37.0	37.0	37.0	37.0
52-53	36.01851851851852	37.0	37.0	37.0	37.0	37.0
54-55	35.940190190190194	37.0	37.0	37.0	37.0	37.0
56-57	35.9532032032032	37.0	37.0	37.0	37.0	37.0
58-59	35.87112112112112	37.0	37.0	37.0	37.0	37.0
60-61	35.94127848939431	37.0	37.0	37.0	37.0	37.0
62-63	35.8970182911551	37.0	37.0	37.0	37.0	37.0
64-65	35.9676772738662	37.0	37.0	37.0	37.0	37.0
66-67	35.84615384615385	37.0	37.0	37.0	37.0	37.0
68-69	35.84947050504175	37.0	37.0	37.0	37.0	37.0
70-71	35.85151743165287	37.0	37.0	37.0	37.0	37.0
72-73	35.79408076247805	37.0	37.0	37.0	37.0	37.0
74-75	35.90798492841375	37.0	37.0	37.0	37.0	37.0
76-77	35.67605188716756	37.0	37.0	37.0	37.0	37.0
78-79	35.74734643779516	37.0	37.0	37.0	37.0	37.0
80-81	35.71841972823351	37.0	37.0	37.0	37.0	37.0
82-83	35.810684789161385	37.0	37.0	37.0	37.0	37.0
84-85	35.74106415494813	37.0	37.0	37.0	37.0	37.0
86-87	35.55164571084174	37.0	37.0	37.0	37.0	37.0
88-89	35.5571128696275	37.0	37.0	37.0	37.0	37.0
90-91	35.615673370038124	37.0	37.0	37.0	37.0	37.0
92-93	35.59134733250279	37.0	37.0	37.0	37.0	37.0
94-95	35.49475641261228	37.0	37.0	37.0	37.0	37.0
96-97	35.392832922960324	37.0	37.0	37.0	37.0	37.0
98-99	35.524261366723785	37.0	37.0	37.0	37.0	37.0
100-101	35.87737007424329	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	1.0
25	3.0
26	4.0
27	17.0
28	20.0
29	32.0
30	35.0
31	38.0
32	62.0
33	114.0
34	206.0
35	540.0
36	2394.0
37	531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.8	18.575	16.575	27.05
2	27.224999999999998	24.25	27.3	21.224999999999998
3	21.775	29.925	28.15	20.150000000000002
4	25.974999999999998	33.625	23.575	16.825000000000003
5	25.85	36.725	20.3	17.125
6	21.95	33.2	26.275	18.575
7	19.875	21.95	38.525	19.650000000000002
8	21.45	24.6	26.474999999999998	27.474999999999998
9	23.075000000000003	24.375	27.650000000000002	24.9
10-11	26.137500000000003	32.4375	20.837500000000002	20.5875
12-13	21.3	26.8625	27.437499999999996	24.4
14-15	20.5375	26.375	30.4375	22.650000000000002
16-17	21.175	27.3625	27.462500000000002	24.0
18-19	22.225	27.4125	27.287499999999998	23.075000000000003
20-21	23.1375	29.6875	27.025	20.150000000000002
22-23	24.337500000000002	28.1875	23.599999999999998	23.875
24-25	20.0375	28.787499999999998	27.9125	23.2625
26-27	22.425	30.587500000000002	25.4625	21.525
28-29	23.175	30.825000000000003	23.599999999999998	22.400000000000002
30-31	22.287499999999998	25.7875	30.062499999999996	21.8625
32-33	23.2625	26.325	27.525	22.8875
34-35	25.7	26.8	25.1	22.400000000000002
36-37	25.034387895460796	28.185569588595722	28.223083656371138	18.55695885957234
38-39	23.899449724862432	28.039019509754876	27.213606803401703	20.847923961980992
40-41	21.473236618309155	26.575787893946973	28.70185092546273	23.24912456228114
42-43	23.024012006003	27.238619309654826	25.950475237618807	23.78689344672336
44-45	24.574787393696848	28.714357178589296	25.42521260630315	21.285642821410704
46-47	23.24912456228114	29.364682341170585	25.900450225112557	21.48574287143572
48-49	21.80385288966725	26.80760570427821	30.010007505629222	21.37853390042532
50-51	20.302916510201527	28.939792214294656	25.835523845287273	24.921767430216548
52-53	22.52252252252252	31.343843843843842	25.663163163163162	20.47047047047047
54-55	20.195195195195197	27.489989989989986	29.517017017017018	22.7977977977978
56-57	20.683183183183182	28.953953953953953	27.05205205205205	23.31081081081081
58-59	22.8978978978979	27.402402402402405	28.666166166166168	21.033533533533532
60-61	21.960686114936774	28.18329785902091	25.516464254413425	24.339551771628898
62-63	20.12027060886996	27.123527937860185	29.892257579554	22.86394387371586
64-65	24.354798296166376	24.71811576046104	28.238536707592083	22.688549235780506
66-67	22.776246554748184	27.023302430468554	26.43447757454272	23.765973440240543
68-69	21.155533274846473	27.472114300037596	25.930567740318335	25.441784684797593
70-71	22.297466766992727	28.103837471783294	25.746175068974164	23.85252069224981
72-73	24.02157551430005	26.655795283492225	26.60561966884094	22.717009533366785
74-75	18.72724990586168	29.860675285552908	28.78122254298983	22.630852265595582
76-77	22.66046979022736	27.471423188041705	27.00665745509358	22.861449566637358
78-79	21.782302664655607	27.023629964806435	27.036199095022624	24.157868275515334
80-81	22.21942627075994	30.359838953195773	26.421741318570707	20.998993457473578
82-83	22.860020140986908	27.177744209466265	25.88116817724068	24.08106747230614
84-85	19.76289569933157	29.73893303064699	27.74624795056123	22.75192331946021
86-87	22.734158040898762	29.664226205503663	27.61928805857107	19.98232769502651
88-89	22.33476829577108	31.780197518359078	24.664472018232463	21.22056216763738
90-91	20.282119710255433	28.78383530308807	27.792603888677085	23.141441097979413
92-93	23.413204180474125	24.968136630130005	28.15447361712975	23.46418557226612
94-95	22.933196826209368	29.012029690299464	26.196570258510366	21.858203224980805
96-97	22.72084351292272	29.72868715442973	25.794007972225796	21.756461360421756
98-99	22.21783741120758	26.716653512233624	27.584846093133386	23.480662983425415
100-101	19.51880718400542	13.385293120975941	33.463232802439855	33.63266689257879
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	0.5
20	2.0
21	2.5
22	2.0
23	1.5
24	2.5
25	4.0
26	4.5
27	5.0
28	6.5
29	8.0
30	10.5
31	10.0
32	10.5
33	16.5
34	17.5
35	22.0
36	35.0
37	84.5
38	125.0
39	169.5
40	194.5
41	205.0
42	221.0
43	228.5
44	258.0
45	244.0
46	293.0
47	365.0
48	321.0
49	230.0
50	160.0
51	152.5
52	179.5
53	175.5
54	108.0
55	35.0
56	26.0
57	22.5
58	7.5
59	10.5
60	15.0
61	11.0
62	9.0
63	6.0
64	1.0
65	1.0
66	1.5
67	0.5
68	0.0
69	0.0
70	0.0
71	0.5
72	0.5
73	0.0
74	0.0
75	0.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.03753753753753754
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.025081514923501375
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
34-35	1.0
36-37	1.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.0
48-49	1.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	1.0
60-61	4.0
62-63	0.0
64-65	0.0
66-67	1.0
68-69	3.0
70-71	0.0
72-73	2.0
74-75	4.0
76-77	2.0
78-79	5.0
80-81	1.0
82-83	8.0
84-85	1.0
86-87	11.0
88-89	14.0
90-91	12.0
92-93	18.0
94-95	14.0
96-97	60.0
98-99	333.0
100-101	3502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.35643564356435	22.75
2	12.376237623762377	8.75
3	5.445544554455446	5.775
4	3.818953323903819	5.4
5	2.6166902404526167	4.625
6	1.9094766619519095	4.05
7	1.5558698727015559	3.85
8	1.1315417256011315	3.2
9	0.9193776520509194	2.9250000000000003
>10	5.728429985855729	35.85
>50	0.14144271570014144	2.825
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	62	1.55	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	51	1.275	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	46	1.15	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	37	0.9249999999999999	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	33	0.8250000000000001	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	32	0.8	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	32	0.8	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	32	0.8	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	31	0.775	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	30	0.75	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	29	0.7250000000000001	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	28	0.7000000000000001	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	27	0.675	No Hit
GGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCT	27	0.675	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	26	0.65	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	25	0.625	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	24	0.6	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	23	0.575	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	23	0.575	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	23	0.575	No Hit
AGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTC	23	0.575	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	23	0.575	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	22	0.5499999999999999	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	22	0.5499999999999999	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	21	0.525	No Hit
GGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTT	21	0.525	No Hit
GTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAA	21	0.525	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	21	0.525	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	20	0.5	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	20	0.5	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	19	0.475	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	19	0.475	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	19	0.475	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	17	0.42500000000000004	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	17	0.42500000000000004	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	17	0.42500000000000004	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	16	0.4	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	16	0.4	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	15	0.375	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	15	0.375	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	15	0.375	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	15	0.375	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	15	0.375	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	15	0.375	No Hit
GGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	15	0.375	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	15	0.375	No Hit
GAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGC	15	0.375	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	14	0.35000000000000003	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	14	0.35000000000000003	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	14	0.35000000000000003	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	14	0.35000000000000003	No Hit
CGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCT	13	0.325	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	13	0.325	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	13	0.325	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	13	0.325	No Hit
GGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCA	13	0.325	No Hit
GGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGA	13	0.325	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	13	0.325	No Hit
GAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGG	13	0.325	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	13	0.325	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	13	0.325	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	12	0.3	No Hit
TATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAA	12	0.3	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	12	0.3	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	11	0.27499999999999997	No Hit
GGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTG	11	0.27499999999999997	No Hit
TGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATA	11	0.27499999999999997	No Hit
GCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTA	11	0.27499999999999997	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	11	0.27499999999999997	No Hit
TATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAAT	10	0.25	No Hit
CAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTAC	10	0.25	No Hit
TGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCA	10	0.25	No Hit
GTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCA	10	0.25	No Hit
TGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAG	10	0.25	No Hit
CTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	10	0.25	No Hit
ATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAG	10	0.25	No Hit
CAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCT	10	0.25	No Hit
GCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTT	10	0.25	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	10	0.25	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	10	0.25	No Hit
CCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGAT	10	0.25	No Hit
CGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTGAGAAAAG	10	0.25	No Hit
TGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCT	10	0.25	No Hit
GATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATT	9	0.22499999999999998	No Hit
TGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAA	9	0.22499999999999998	No Hit
GCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTG	9	0.22499999999999998	No Hit
GAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAG	9	0.22499999999999998	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	9	0.22499999999999998	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	9	0.22499999999999998	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	9	0.22499999999999998	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGT	9	0.22499999999999998	No Hit
TGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTC	9	0.22499999999999998	No Hit
TACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGC	9	0.22499999999999998	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	9	0.22499999999999998	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	9	0.22499999999999998	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGCACT	9	0.22499999999999998	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	8	0.2	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	8	0.2	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGACC	8	0.2	No Hit
GCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCA	8	0.2	No Hit
CCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGAC	8	0.2	No Hit
TATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGC	8	0.2	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	8	0.2	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	8	0.2	No Hit
TGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATG	8	0.2	No Hit
CTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACT	8	0.2	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	8	0.2	No Hit
TAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTC	8	0.2	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	8	0.2	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	8	0.2	No Hit
AATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAA	8	0.2	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	8	0.2	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	7	0.17500000000000002	No Hit
CGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACT	7	0.17500000000000002	No Hit
TTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGG	7	0.17500000000000002	No Hit
TCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATG	7	0.17500000000000002	No Hit
TTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAA	7	0.17500000000000002	No Hit
TGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCT	7	0.17500000000000002	No Hit
TCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGA	7	0.17500000000000002	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	7	0.17500000000000002	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	7	0.17500000000000002	No Hit
ATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTG	7	0.17500000000000002	No Hit
CTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTAC	7	0.17500000000000002	No Hit
CAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCC	7	0.17500000000000002	No Hit
ATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTC	7	0.17500000000000002	No Hit
GATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGA	7	0.17500000000000002	No Hit
CATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTG	7	0.17500000000000002	No Hit
TTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAA	7	0.17500000000000002	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	7	0.17500000000000002	No Hit
GTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCA	7	0.17500000000000002	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	7	0.17500000000000002	No Hit
AATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCC	7	0.17500000000000002	No Hit
TAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAA	7	0.17500000000000002	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	7	0.17500000000000002	No Hit
GCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTG	6	0.15	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	6	0.15	No Hit
GCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGG	6	0.15	No Hit
CTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCC	6	0.15	No Hit
CTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGT	6	0.15	No Hit
CCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGA	6	0.15	No Hit
AATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACC	6	0.15	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	6	0.15	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	6	0.15	No Hit
AATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCG	6	0.15	No Hit
GAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGC	6	0.15	No Hit
ATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAG	6	0.15	No Hit
ACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCA	6	0.15	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	6	0.15	No Hit
CAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACA	6	0.15	No Hit
CTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGC	6	0.15	No Hit
TGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAG	6	0.15	No Hit
CAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGA	6	0.15	No Hit
ATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGA	6	0.15	No Hit
CCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGG	6	0.15	No Hit
ATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAA	6	0.15	No Hit
GAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCT	6	0.15	No Hit
GCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATA	6	0.15	No Hit
ATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTT	6	0.15	No Hit
ATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTA	6	0.15	No Hit
ACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGC	6	0.15	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	6	0.15	No Hit
GCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATA	5	0.125	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	5	0.125	No Hit
CTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATC	5	0.125	No Hit
GTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCA	5	0.125	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	5	0.125	No Hit
TTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAA	5	0.125	No Hit
TGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAAT	5	0.125	No Hit
CAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAA	5	0.125	No Hit
TGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTA	5	0.125	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	5	0.125	No Hit
GTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCT	5	0.125	No Hit
CCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTA	5	0.125	No Hit
AGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCT	5	0.125	No Hit
ATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGC	5	0.125	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	5	0.125	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	5	0.125	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	5	0.125	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	5	0.125	No Hit
AAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCG	5	0.125	No Hit
CTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGG	5	0.125	No Hit
GCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGC	5	0.125	No Hit
AAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAA	5	0.125	No Hit
AACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTT	5	0.125	No Hit
TGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAG	5	0.125	No Hit
GTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAA	5	0.125	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	5	0.125	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	5	0.125	No Hit
CCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCC	5	0.125	No Hit
AGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGT	5	0.125	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	5	0.125	No Hit
AGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATG	5	0.125	No Hit
GGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGC	5	0.125	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	5	0.125	No Hit
CCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCT	5	0.125	No Hit
GTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATAT	5	0.125	No Hit
GTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGG	5	0.125	No Hit
GCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2026-05-21T14:08:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T14:08:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T14:08:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T14:09:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-05-21T14:10:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
Read 16660009 spots for SRR25656851.sra
Written 16660009 spots for SRR25656851.sra
SRR ids: ['SRR25656851.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_kiihgygd
SRR25656851.sra spots: 133280072
blocks: [[1, 16660009], [16660010, 33320018], [33320019, 49980027], [49980028, 66640036], [66640037, 83300045], [83300046, 99960054], [99960055, 116620063], [116620064, 133280072]]
SRR25656851 file size 32073312
SRR25656851 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25656851 SRR25656851_1.fastq SRR25656851_2.fastq
Input file:	SRR25656851_1.fastq
Paired file:	SRR25656851_2.fastq
trimmed:	SRR25656851-trimmed-pair1.fastq, SRR25656851-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 14:24:23 2026 >> started

Thu May 21 14:27:53 2026 >> done (210.117s)
133280072 read pairs processed; of these:
        2 ( 0.00%) short read pairs filtered out after trimming by size control
      268 ( 0.00%) empty read pairs filtered out after trimming by size control
133279802 (100.00%) read pairs available; of these:
    56706 ( 0.04%) trimmed read pairs available after processing
133223096 (99.96%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	        1	  0.00%
 19	        0	  0.00%
 20	       42	  0.00%
 21	       77	  0.00%
 22	       79	  0.00%
 23	      118	  0.00%
 24	      143	  0.00%
 25	      156	  0.00%
 26	      184	  0.00%
 27	      259	  0.00%
 28	      304	  0.00%
 29	      349	  0.00%
 30	      411	  0.00%
 31	      452	  0.00%
 32	      523	  0.00%
 33	      552	  0.00%
 34	      626	  0.00%
 35	      678	  0.00%
 36	      808	  0.00%
 37	      793	  0.00%
 38	      981	  0.00%
 39	     1108	  0.00%
 40	     1368	  0.00%
 41	     1566	  0.00%
 42	     1697	  0.00%
 43	     1790	  0.00%
 44	     1832	  0.00%
 45	     1986	  0.00%
 46	     2430	  0.00%
 47	     2929	  0.00%
 48	     3431	  0.00%
 49	     4219	  0.00%
 50	     4453	  0.00%
 51	     4828	  0.00%
 52	     5403	  0.00%
 53	     6040	  0.00%
 54	     6335	  0.00%
 55	     7019	  0.01%
 56	     7518	  0.01%
 57	     8214	  0.01%
 58	     9257	  0.01%
 59	    17442	  0.01%
 60	    85375	  0.06%
 61	   103534	  0.08%
 62	    89989	  0.07%
 63	    75393	  0.06%
 64	    83211	  0.06%
 65	    66100	  0.05%
 66	    53644	  0.04%
 67	    62928	  0.05%
 68	    50981	  0.04%
 69	    50636	  0.04%
 70	    55830	  0.04%
 71	    51702	  0.04%
 72	    53875	  0.04%
 73	    55508	  0.04%
 74	    59274	  0.04%
 75	    61940	  0.05%
 76	    65196	  0.05%
 77	    67725	  0.05%
 78	    72067	  0.05%
 79	    79257	  0.06%
 80	    88514	  0.07%
 81	    96776	  0.07%
 82	   107447	  0.08%
 83	   117072	  0.09%
 84	   123919	  0.09%
 85	   137786	  0.10%
 86	   153022	  0.11%
 87	   166259	  0.12%
 88	   170533	  0.13%
 89	   189042	  0.14%
 90	   214282	  0.16%
 91	   247750	  0.19%
 92	   270866	  0.20%
 93	   304395	  0.23%
 94	   343194	  0.26%
 95	   375218	  0.28%
 96	   460075	  0.35%
 97	   591987	  0.44%
 98	  1614309	  1.21%
 99	 10556160	  7.92%
100	 56307738	 42.25%
101	 59190892	 44.41%
133279802 reads passed initial QC


criterion=sequence-density
sequence-density=1.46
sequence-density-rank=1
fanout-score=6.78
fanout-score-rank=6
prefix-density=2.57
prefix-fanout=3.8
sequence=ACCAGCTGGGCCAG


criterion=fanout-score
sequence-density=1.23
sequence-density-rank=10
fanout-score=32.84
fanout-score-rank=1
prefix-density=4.71
prefix-fanout=8.6
sequence=TTCACCCTTTGG


criterion=sequence-density
sequence-density=1.84
sequence-density-rank=1
fanout-score=3.00
fanout-score-rank=16
prefix-density=1.67
prefix-fanout=3.0
sequence=GGTGAACCAGGTG


criterion=fanout-score
sequence-density=1.24
sequence-density-rank=12
fanout-score=10.25
fanout-score-rank=1
prefix-density=3.74
prefix-fanout=3.4
sequence=CCAGGACCTCCTGG
SRR25656851 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 14:28:12
                             Started mapping on |	May 21 14:28:12
                                    Finished on |	May 21 14:39:23
       Mapping speed, Million of reads per hour |	715.06

                          Number of input reads |	133279802
                      Average input read length |	199
                                    UNIQUE READS:
                   Uniquely mapped reads number |	95234238
                        Uniquely mapped reads % |	71.45%
                          Average mapped length |	198.63
                       Number of splices: Total |	59568804
            Number of splices: Annotated (sjdb) |	59164897
                       Number of splices: GT/AG |	58825490
                       Number of splices: GC/AG |	592037
                       Number of splices: AT/AC |	54790
               Number of splices: Non-canonical |	96487
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.48
                        Insertion rate per base |	0.03%
                       Insertion average length |	1.64
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	22542680
             % of reads mapped to multiple loci |	16.91%
        Number of reads mapped to too many loci |	988081
             % of reads mapped to too many loci |	0.74%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	10.78%
                     % of reads unmapped: other |	0.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	16006441	16006441	16006441
N_multimapping	22542680	22542680	22542680
N_noFeature	10621059	90309720	11276777
N_ambiguous	6060495	62814	1942180
UnstrandedReadsAssigned:78552684 PositiveStrandReadsAssigned:4861704 NegativeStrandReadsAssigned:82015281
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=100 echo kmer=95
SRR25656851 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25656851-trimmed-pair1.fastq
                             SRR25656851-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 133,279,802 reads, 74,868,408 reads pseudoaligned
[quant] estimated average fragment length: 190.781
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,397 rounds

 109283 SRR25656851.ke.tsv
  52636 SRR25656851.se.tsv
 161919 total
==> SRR25656851.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	2	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	1	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	2	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25656851.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25656851 completed mapping pipeline successfully
