Starting /dee2/code/volunteer_pipeline.sh SRR25656854
    current disk space = 1046122256
    free memory = 192588492 
SRR25656854 SRAfilesize
105bd91b560fce771d2aa20e83204b02  SRR25656854.sra
SRR25656854.sra file validated
SRR25656854 is paired end
SRR25656854 is conventional basespace
SRR25656854 read1 length is 56-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	56-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.611	37.0	37.0	37.0	37.0	37.0
2	36.52875	37.0	37.0	37.0	37.0	37.0
3	36.603	37.0	37.0	37.0	37.0	37.0
4	36.623	37.0	37.0	37.0	37.0	37.0
5	36.5635	37.0	37.0	37.0	37.0	37.0
6	36.648	37.0	37.0	37.0	37.0	37.0
7	36.478	37.0	37.0	37.0	37.0	37.0
8	36.56425	37.0	37.0	37.0	37.0	37.0
9	36.564	37.0	37.0	37.0	37.0	37.0
10-11	36.60925	37.0	37.0	37.0	37.0	37.0
12-13	36.57875	37.0	37.0	37.0	37.0	37.0
14-15	36.56375	37.0	37.0	37.0	37.0	37.0
16-17	36.5385	37.0	37.0	37.0	37.0	37.0
18-19	36.538	37.0	37.0	37.0	37.0	37.0
20-21	36.5565	37.0	37.0	37.0	37.0	37.0
22-23	36.56525	37.0	37.0	37.0	37.0	37.0
24-25	36.558	37.0	37.0	37.0	37.0	37.0
26-27	36.519999999999996	37.0	37.0	37.0	37.0	37.0
28-29	36.49275	37.0	37.0	37.0	37.0	37.0
30-31	36.561499999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.5175	37.0	37.0	37.0	37.0	37.0
34-35	36.52225	37.0	37.0	37.0	37.0	37.0
36-37	36.517250000000004	37.0	37.0	37.0	37.0	37.0
38-39	36.51175	37.0	37.0	37.0	37.0	37.0
40-41	36.509249999999994	37.0	37.0	37.0	37.0	37.0
42-43	36.42525	37.0	37.0	37.0	37.0	37.0
44-45	36.4685	37.0	37.0	37.0	37.0	37.0
46-47	36.463750000000005	37.0	37.0	37.0	37.0	37.0
48-49	36.44375	37.0	37.0	37.0	37.0	37.0
50-51	36.42375	37.0	37.0	37.0	37.0	37.0
52-53	36.407	37.0	37.0	37.0	37.0	37.0
54-55	36.4045	37.0	37.0	37.0	37.0	37.0
56-57	36.4529279819955	37.0	37.0	37.0	37.0	37.0
58-59	36.39772108401131	37.0	37.0	37.0	37.0	37.0
60-61	36.4520890668001	37.0	37.0	37.0	37.0	37.0
62-63	36.3953953953954	37.0	37.0	37.0	37.0	37.0
64-65	36.335753630445666	37.0	37.0	37.0	37.0	37.0
66-67	36.38417230152767	37.0	37.0	37.0	37.0	37.0
68-69	36.3251503006012	37.0	37.0	37.0	37.0	37.0
70-71	36.34719327760644	37.0	37.0	37.0	37.0	37.0
72-73	36.35323420726186	37.0	37.0	37.0	37.0	37.0
74-75	36.32881496782024	37.0	37.0	37.0	37.0	37.0
76-77	36.33669915827862	37.0	37.0	37.0	37.0	37.0
78-79	36.337106918238995	37.0	37.0	37.0	37.0	37.0
80-81	36.32591573473995	37.0	37.0	37.0	37.0	37.0
82-83	36.306995603705246	37.0	37.0	37.0	37.0	37.0
84-85	36.32524601530182	37.0	37.0	37.0	37.0	37.0
86-87	36.386635140069636	37.0	37.0	37.0	37.0	37.0
88-89	36.363949008914474	37.0	37.0	37.0	37.0	37.0
90-91	36.25283151692946	37.0	37.0	37.0	37.0	37.0
92-93	36.15927390856821	37.0	37.0	37.0	37.0	37.0
94-95	36.120714197138014	37.0	37.0	37.0	37.0	37.0
96-97	36.153064727416165	37.0	37.0	37.0	37.0	37.0
98-99	36.21798165433507	37.0	37.0	37.0	37.0	37.0
100-101	36.4987942725854	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	2.0
26	3.0
27	8.0
28	11.0
29	13.0
30	28.0
31	33.0
32	41.0
33	58.0
34	74.0
35	210.0
36	2307.0
37	1211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.575	9.65	13.025	43.75
2	21.215911933950462	22.71703777833375	29.622216662496875	26.444833625218916
3	23.65	27.3	18.75	30.3
4	26.85	37.95	18.8	16.400000000000002
5	31.674999999999997	33.625	21.875	12.825000000000001
6	26.05	37.65	19.875	16.425
7	16.400000000000002	27.175	39.975	16.45
8	18.16362271703778	27.62071553665249	33.52514385789342	20.690517888416313
9	16.875	22.125	36.95	24.05
10-11	20.4625	36.7	22.7375	20.1
12-13	21.15	29.862499999999997	27.55	21.4375
14-15	23.6375	25.2625	24.6875	26.4125
16-17	23.849999999999998	28.625	23.0	24.525
18-19	22.625	27.525	23.549999999999997	26.3
20-21	19.537499999999998	29.099999999999998	26.5625	24.8
22-23	22.4625	28.050000000000004	26.5625	22.925
24-25	24.4375	25.3125	30.7375	19.5125
26-27	24.087500000000002	25.4875	24.9375	25.4875
28-29	24.349999999999998	25.75	25.45	24.45
30-31	22.25	26.9125	26.387500000000003	24.45
32-33	19.3375	32.65	22.787499999999998	25.224999999999998
34-35	23.25	32.337500000000006	22.662499999999998	21.75
36-37	24.2375	26.7625	25.324999999999996	23.674999999999997
38-39	22.95	23.5125	28.7	24.837500000000002
40-41	21.25	23.4375	31.45	23.8625
42-43	25.0625	22.325	31.412499999999998	21.2
44-45	25.5625	23.7125	28.375	22.35
46-47	28.212500000000002	24.3875	25.637500000000003	21.762500000000003
48-49	25.6	29.4	21.8	23.200000000000003
50-51	25.6125	31.2875	19.825	23.275000000000002
52-53	22.7	26.787499999999998	25.8625	24.65
54-55	25.074999999999996	23.275000000000002	29.725	21.925
56-57	24.49056132016502	23.39042380297537	32.36654581822728	19.75246905863233
58-59	19.92246123061531	24.68734367183592	31.015507753876935	24.374687343671837
60-61	28.809106830122595	30.88566424818614	21.766324743557668	18.5389041781336
62-63	26.564064064064063	30.205205205205203	21.996996996996998	21.233733733733732
64-65	25.951427140711065	23.034551827741613	28.179769654481724	22.8342513770656
66-67	26.93463561232156	27.435512146255945	24.768344603055347	20.861507638367144
68-69	25.56362725450902	25.025050100200403	27.029058116232463	22.382264529058116
70-71	22.252850519984964	30.44731236687132	27.364991855657184	19.93484525748653
72-73	20.26332288401254	24.815047021943574	32.840125391849526	22.08150470219436
74-75	23.447497177267596	24.313135114791116	29.10550746455903	23.13386024338226
76-77	29.447236180904525	29.635678391959797	21.331658291457288	19.585427135678394
78-79	28.67924528301887	31.886792452830186	20.11320754716981	19.32075471698113
80-81	27.052896725440807	27.92191435768262	24.105793450881613	20.91939546599496
82-83	24.65615141955836	25.993690851735018	28.403785488958988	20.946372239747635
84-85	20.212362533181647	27.49336367083807	30.704082922512953	21.590190873467325
86-87	21.526369168357	27.066430020283978	28.65111561866126	22.75608519269777
88-89	20.124666073018698	27.579188398422595	28.43149726497901	23.864648263579696
90-91	27.35391681551416	24.1260525644297	28.489410563919364	20.03062005613677
92-93	30.405837920880806	27.35885289975675	23.44130072974011	18.794008449622325
94-95	27.869906157603808	26.23730556626816	27.265715387581952	18.62707288854609
96-97	24.39339184305627	28.510583376355186	28.471863706763035	18.624161073825505
98-99	21.658549783549784	27.421536796536795	32.95454545454545	17.965367965367964
100-101	22.154919236417033	11.508810572687226	36.39867841409692	29.93759177679883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.0
21	0.0
22	0.0
23	0.0
24	1.0
25	1.0
26	0.0
27	1.0
28	1.5
29	2.0
30	3.5
31	3.5
32	5.0
33	9.0
34	15.0
35	17.5
36	21.0
37	43.5
38	66.5
39	117.0
40	155.0
41	159.0
42	147.5
43	194.0
44	347.0
45	329.0
46	364.0
47	405.0
48	312.0
49	244.5
50	166.5
51	168.0
52	148.0
53	133.0
54	92.0
55	66.5
56	102.5
57	65.0
58	25.0
59	25.0
60	32.5
61	25.5
62	5.0
63	2.0
64	1.5
65	1.0
66	0.5
67	0.5
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.075
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.075
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
56	1.0
57	0.0
58	2.0
59	0.0
60	0.0
61	1.0
62	0.0
63	2.0
64	0.0
65	1.0
66	0.0
67	1.0
68	0.0
69	1.0
70	1.0
71	2.0
72	1.0
73	1.0
74	1.0
75	3.0
76	4.0
77	3.0
78	0.0
79	3.0
80	4.0
81	4.0
82	3.0
83	4.0
84	3.0
85	6.0
86	8.0
87	6.0
88	7.0
89	5.0
90	6.0
91	5.0
92	11.0
93	8.0
94	5.0
95	9.0
96	8.0
97	40.0
98	268.0
99	332.0
100	1012.0
101	2218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.199999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.815602836879435	17.150000000000002
2	16.22340425531915	9.15
3	6.294326241134751	5.325
4	2.8368794326241136	3.2
5	2.304964539007092	3.25
6	1.3297872340425532	2.25
7	1.0638297872340425	2.1
8	1.152482269503546	2.6
9	0.7092198581560284	1.7999999999999998
>10	6.560283687943262	35.699999999999996
>50	0.5319148936170213	10.525
>100	0.1773049645390071	6.950000000000001
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	142	3.55	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	136	3.4000000000000004	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	92	2.3	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	85	2.125	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	67	1.675	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	60	1.5	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	59	1.4749999999999999	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	58	1.4500000000000002	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	50	1.25	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	49	1.225	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	47	1.175	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	41	1.0250000000000001	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	40	1.0	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	38	0.95	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	38	0.95	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	36	0.8999999999999999	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	36	0.8999999999999999	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	34	0.8500000000000001	No Hit
CTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGG	33	0.8250000000000001	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	29	0.7250000000000001	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	28	0.7000000000000001	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	27	0.675	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	26	0.65	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	26	0.65	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	26	0.65	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	26	0.65	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	23	0.575	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	22	0.5499999999999999	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	22	0.5499999999999999	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	21	0.525	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	21	0.525	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	21	0.525	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	20	0.5	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	20	0.5	No Hit
CCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTA	20	0.5	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	19	0.475	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	18	0.44999999999999996	No Hit
GTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTC	18	0.44999999999999996	No Hit
TCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGG	17	0.42500000000000004	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	17	0.42500000000000004	No Hit
TTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCA	16	0.4	No Hit
GGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGACCA	16	0.4	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	16	0.4	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	15	0.375	No Hit
TTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTC	15	0.375	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	15	0.375	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	15	0.375	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	14	0.35000000000000003	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	14	0.35000000000000003	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	14	0.35000000000000003	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	13	0.325	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	13	0.325	No Hit
AACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTG	13	0.325	No Hit
TAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAA	13	0.325	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	13	0.325	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	13	0.325	No Hit
CTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCG	13	0.325	No Hit
GTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGA	13	0.325	No Hit
CTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGCC	12	0.3	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	12	0.3	No Hit
CCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGT	12	0.3	No Hit
AGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCA	12	0.3	No Hit
TCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTT	12	0.3	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	12	0.3	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	12	0.3	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	12	0.3	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	12	0.3	No Hit
GGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGAGA	11	0.27499999999999997	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	11	0.27499999999999997	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	11	0.27499999999999997	No Hit
ATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATC	11	0.27499999999999997	No Hit
AGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTC	11	0.27499999999999997	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	11	0.27499999999999997	No Hit
AGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTA	11	0.27499999999999997	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	10	0.25	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	10	0.25	No Hit
AGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAA	10	0.25	No Hit
GGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	10	0.25	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	10	0.25	No Hit
TCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAGGC	10	0.25	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	10	0.25	No Hit
CTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAA	10	0.25	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	9	0.22499999999999998	No Hit
TGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCG	9	0.22499999999999998	No Hit
GTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGG	9	0.22499999999999998	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	9	0.22499999999999998	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	9	0.22499999999999998	No Hit
CCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAG	9	0.22499999999999998	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	9	0.22499999999999998	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	9	0.22499999999999998	No Hit
CAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCT	8	0.2	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	8	0.2	No Hit
TTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGAC	8	0.2	No Hit
CTCCATTTGGCAGATGGTGAAACTGAGGCAGAGAATAAATTGCATGCCAA	8	0.2	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	8	0.2	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	8	0.2	No Hit
CATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGAT	8	0.2	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	8	0.2	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	8	0.2	No Hit
GTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGC	8	0.2	No Hit
CTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATAT	8	0.2	No Hit
GTGGGGGCAGATGCAGGCACACATGGAGGCCAGATGCAGGCACACATGGA	8	0.2	No Hit
TAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCA	8	0.2	No Hit
GTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAG	7	0.17500000000000002	No Hit
GTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAG	7	0.17500000000000002	No Hit
AGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAA	7	0.17500000000000002	No Hit
ATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAA	7	0.17500000000000002	No Hit
TGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCA	7	0.17500000000000002	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	7	0.17500000000000002	No Hit
GATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCAT	7	0.17500000000000002	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	7	0.17500000000000002	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	7	0.17500000000000002	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	7	0.17500000000000002	No Hit
GACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGG	7	0.17500000000000002	No Hit
CGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTG	7	0.17500000000000002	No Hit
ATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	6	0.15	No Hit
GGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTA	6	0.15	No Hit
CAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTT	6	0.15	No Hit
AGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGT	6	0.15	No Hit
TCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTG	6	0.15	No Hit
TGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCT	6	0.15	No Hit
AAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAG	6	0.15	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	6	0.15	No Hit
GCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCAC	6	0.15	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	6	0.15	No Hit
GAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTT	6	0.15	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	6	0.15	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	6	0.15	No Hit
TTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCT	6	0.15	No Hit
TGAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCC	6	0.15	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	5	0.125	No Hit
CAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGC	5	0.125	No Hit
GCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCCCTAGACTTCT	5	0.125	No Hit
GACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAG	5	0.125	No Hit
CTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCCCTAGACT	5	0.125	No Hit
CGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAAT	5	0.125	No Hit
GCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTAT	5	0.125	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	5	0.125	No Hit
GTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGC	5	0.125	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	5	0.125	No Hit
CAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGT	5	0.125	No Hit
GCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGAGCATTCT	5	0.125	No Hit
GTCAGATGTCTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGT	5	0.125	No Hit
TGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAG	5	0.125	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	5	0.125	No Hit
CAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGAC	5	0.125	No Hit
GGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATA	5	0.125	No Hit
GTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGT	5	0.125	No Hit
GAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCA	5	0.125	No Hit
ATTACCACTGGGTTATTATCACGAATGGCCGATTTAATAAGTCCTTTTGC	5	0.125	No Hit
CCAGCGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAAT	5	0.125	No Hit
CTCAGGAGGCTTGTAGAAAAGCTCAGCTTGACACGGTCCAGTTCCACCTT	5	0.125	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	5	0.125	No Hit
GTGATCTTCAAAGGTTGGGTTGTAGGAGGCTCCTGCAGGAATCACCTCCA	5	0.125	No Hit
CCTCTTACTTTAGAAATGGGATGTGGGGGCAGATGCAGGCACACATGGAG	5	0.125	No Hit
GGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGACC	25	0.004838246	56.475	6
TGGTAAC	25	0.004838246	56.475	1
TAACAGA	25	0.004838246	56.475	4
CAGACCC	25	0.004838246	56.475	7
AACAGAC	25	0.004838246	56.475	5
AGACCCA	25	0.004838246	56.475	8
GACCCAA	25	0.004838246	56.475	9
GTAACAG	25	0.004838246	56.475	3
GGTAACA	25	0.004838246	56.475	2
ACCCAAA	25	0.0015925721	37.649998	10-11
CACAAGA	80	3.960148E-4	29.414062	1
ACAAGAA	80	3.960148E-4	29.414062	2
CAAGAAT	95	0.001082805	24.769735	3
AGAATCC	95	0.001082805	24.769735	5
AAGAATC	95	0.001082805	24.769735	4
GAATCCA	95	0.001082805	24.769735	6
TCCATGT	95	0.001082805	24.769735	9
AATCCAT	100	0.0014606793	23.53125	7
ATCCATG	100	0.0014606793	23.53125	8
AGGTTTT	90	1.9969637E-4	22.612614	94-95
>>END_MODULE
SRR25656854 read2 length is 44-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656854_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	44-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3625	37.0	37.0	37.0	37.0	37.0
2	36.44	37.0	37.0	37.0	37.0	37.0
3	36.4655	37.0	37.0	37.0	37.0	37.0
4	36.558	37.0	37.0	37.0	37.0	37.0
5	36.459	37.0	37.0	37.0	37.0	37.0
6	36.446	37.0	37.0	37.0	37.0	37.0
7	36.4885	37.0	37.0	37.0	37.0	37.0
8	36.539	37.0	37.0	37.0	37.0	37.0
9	36.4775	37.0	37.0	37.0	37.0	37.0
10-11	36.496750000000006	37.0	37.0	37.0	37.0	37.0
12-13	36.5055	37.0	37.0	37.0	37.0	37.0
14-15	36.39275	37.0	37.0	37.0	37.0	37.0
16-17	36.468999999999994	37.0	37.0	37.0	37.0	37.0
18-19	36.41075	37.0	37.0	37.0	37.0	37.0
20-21	36.4185	37.0	37.0	37.0	37.0	37.0
22-23	36.4525	37.0	37.0	37.0	37.0	37.0
24-25	36.39375	37.0	37.0	37.0	37.0	37.0
26-27	36.312250000000006	37.0	37.0	37.0	37.0	37.0
28-29	36.39425	37.0	37.0	37.0	37.0	37.0
30-31	36.393	37.0	37.0	37.0	37.0	37.0
32-33	36.427499999999995	37.0	37.0	37.0	37.0	37.0
34-35	36.356750000000005	37.0	37.0	37.0	37.0	37.0
36-37	36.4215	37.0	37.0	37.0	37.0	37.0
38-39	36.25725	37.0	37.0	37.0	37.0	37.0
40-41	36.2635	37.0	37.0	37.0	37.0	37.0
42-43	36.1365	37.0	37.0	37.0	37.0	37.0
44-45	36.25640953988497	37.0	37.0	37.0	37.0	37.0
46-47	36.25406351587897	37.0	37.0	37.0	37.0	37.0
48-49	36.26231557889472	37.0	37.0	37.0	37.0	37.0
50-51	36.22855713928482	37.0	37.0	37.0	37.0	37.0
52-53	36.19729932483121	37.0	37.0	37.0	37.0	37.0
54-55	36.14253563390848	37.0	37.0	37.0	37.0	37.0
56-57	36.16969268580277	37.0	37.0	37.0	37.0	37.0
58-59	36.05029479204067	37.0	37.0	37.0	37.0	37.0
60-61	36.25775775775776	37.0	37.0	37.0	37.0	37.0
62-63	36.21146146146146	37.0	37.0	37.0	37.0	37.0
64-65	36.07611417125689	37.0	37.0	37.0	37.0	37.0
66-67	36.20310543451039	37.0	37.0	37.0	37.0	37.0
68-69	36.14704408817635	37.0	37.0	37.0	37.0	37.0
70-71	36.16452186592766	37.0	37.0	37.0	37.0	37.0
72-73	36.17215639267942	37.0	37.0	37.0	37.0	37.0
74-75	35.973027239000245	37.0	37.0	37.0	37.0	37.0
76-77	36.176333975489	37.0	37.0	37.0	37.0	37.0
78-79	36.13811320754717	37.0	37.0	37.0	37.0	37.0
80-81	36.126765889451974	37.0	37.0	37.0	37.0	37.0
82-83	36.01400664552036	37.0	37.0	37.0	37.0	37.0
84-85	35.96884437119731	37.0	37.0	37.0	37.0	37.0
86-87	36.00513274612916	37.0	37.0	37.0	37.0	37.0
88-89	35.92910776649998	37.0	37.0	37.0	37.0	37.0
90-91	36.06403753831662	37.0	37.0	37.0	37.0	37.0
92-93	35.95580002191994	37.0	37.0	37.0	37.0	37.0
94-95	36.045442291448744	37.0	37.0	37.0	37.0	37.0
96-97	36.014039420690295	37.0	37.0	37.0	37.0	37.0
98-99	36.02534243121504	37.0	37.0	37.0	37.0	37.0
100-101	36.23853424645253	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	1.0
25	3.0
26	4.0
27	12.0
28	15.0
29	15.0
30	23.0
31	34.0
32	44.0
33	75.0
34	133.0
35	362.0
36	2506.0
37	771.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.6	17.125	18.2	33.074999999999996
2	23.65	23.599999999999998	31.125000000000004	21.625
3	21.975	28.1	28.975	20.95
4	25.224999999999998	34.875	23.025000000000002	16.875
5	25.4	35.85	21.2	17.549999999999997
6	21.125	33.324999999999996	25.775	19.775000000000002
7	17.575	21.525	39.300000000000004	21.6
8	19.725	22.8	31.05	26.424999999999997
9	23.05	24.95	27.85	24.15
10-11	23.7	33.7125	20.7375	21.85
12-13	19.8625	28.1375	27.1125	24.887500000000003
14-15	20.7875	26.575	30.662499999999998	21.975
16-17	20.474999999999998	27.775	25.5375	26.2125
18-19	21.925	25.55	27.875	24.65
20-21	21.2	30.6875	27.762500000000003	20.349999999999998
22-23	23.6875	26.875	25.137500000000003	24.3
24-25	17.8875	27.1375	29.349999999999998	25.624999999999996
26-27	21.087500000000002	30.612499999999997	25.35	22.95
28-29	20.8125	30.8	24.0125	24.375
30-31	21.125	24.525	29.675	24.675
32-33	23.0375	25.6	27.8875	23.474999999999998
34-35	26.0375	26.525	22.900000000000002	24.5375
36-37	25.374999999999996	28.15	27.3	19.175
38-39	25.387500000000003	25.8125	26.35	22.45
40-41	18.925	26.4625	29.525000000000002	25.087500000000002
42-43	22.275	26.575	25.137500000000003	26.0125
44-45	24.40305038129766	27.340917614701837	25.978247280910118	22.277784723090384
46-47	22.030507626906726	29.144786196549138	26.18154538634659	22.643160790197552
48-49	22.31807951987997	25.55638909727432	29.144786196549138	22.980745186296573
50-51	19.829957489372344	28.707176794198553	25.968992248062015	25.49387346836709
52-53	21.85546386596649	31.070267566891722	24.656164041010253	22.418104526131533
54-55	19.217304326081518	29.144786196549138	29.2448112028007	22.393098274568644
56-57	20.095035638364386	27.860447667875455	26.7725397023884	25.271976991371766
58-59	21.153365023767826	25.969477107830873	29.522141606204656	23.35501626219665
60-61	22.02202202202202	26.576576576576578	25.763263263263266	25.63813813813814
62-63	20.72072072072072	25.763263263263266	30.16766766766767	23.34834834834835
64-65	22.69654481722584	24.87481221832749	28.217325988983475	24.211316975463195
66-67	23.553719008264462	26.70924117205109	23.779113448534936	25.957926371149508
68-69	19.626753507014026	29.483967935871743	25.325651302605213	25.56362725450902
70-71	22.12755293822829	27.415110888359855	26.099486279914796	24.357849893497054
72-73	24.83381412266399	27.078891257995735	24.26940925623981	23.817885363100462
74-75	19.884581608330194	28.666415757119555	28.064232844059717	23.384769790490527
76-77	23.09045226130653	26.494974874371856	26.19346733668342	24.22110552763819
78-79	23.937106918238992	25.031446540880502	26.465408805031448	24.566037735849054
80-81	22.51889168765743	28.551637279596974	26.624685138539046	22.30478589420655
82-83	24.7192429022082	26.397476340694006	24.315457413249213	24.56782334384858
84-85	20.05056890012642	27.9519595448799	27.63590391908976	24.36156763590392
86-87	21.81080395637839	28.937357342125285	25.767182348465635	23.484656353030687
88-89	21.554905204224458	30.550960682020612	26.975442168214787	20.918691945540143
90-91	21.094946401225116	25.58703420112302	26.965288412455337	26.352730985196526
92-93	22.697579095683363	25.054438324580502	27.270398360445753	24.97758421929038
94-95	22.403809033586413	29.326984944022648	24.835928451936688	23.43327757045425
96-97	20.956690368455074	29.993535875888817	26.645119586296058	22.404654169360054
98-99	23.32272606735614	26.425351365685497	25.643065499867408	24.60885706709096
100-101	19.86209216279852	12.243525058863101	33.181971072990244	34.71241170534813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	1.0
25	2.0
26	1.0
27	1.0
28	1.0
29	1.5
30	3.5
31	8.0
32	10.5
33	12.0
34	12.0
35	13.0
36	28.5
37	103.5
38	146.5
39	149.0
40	181.5
41	196.5
42	200.0
43	205.5
44	222.5
45	215.0
46	260.5
47	364.0
48	374.0
49	244.0
50	129.0
51	155.0
52	190.0
53	203.0
54	154.0
55	71.0
56	49.0
57	32.0
58	20.0
59	15.0
60	14.0
61	12.5
62	9.0
63	5.5
64	3.5
65	2.5
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.5
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.025078369905956112
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
44-45	1.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	1.0
58-59	2.0
60-61	0.0
62-63	2.0
64-65	1.0
66-67	1.0
68-69	1.0
70-71	3.0
72-73	2.0
74-75	4.0
76-77	7.0
78-79	3.0
80-81	8.0
82-83	7.0
84-85	10.0
86-87	14.0
88-89	12.0
90-91	11.0
92-93	22.0
94-95	15.0
96-97	62.0
98-99	338.0
100-101	3473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.025000000000002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.9483204134367	17.4
2	13.350559862187769	7.75
3	5.943152454780361	5.175
4	3.6175710594315245	4.2
5	2.4117140396210166	3.5000000000000004
6	1.636520241171404	2.85
7	1.8087855297157622	3.675
8	1.03359173126615	2.4
9	1.119724375538329	2.9250000000000003
>10	8.957795004306632	46.650000000000006
>50	0.17226528854435832	3.4750000000000005
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	70	1.7500000000000002	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	69	1.725	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	49	1.225	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	47	1.175	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	37	0.9249999999999999	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	37	0.9249999999999999	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	34	0.8500000000000001	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	33	0.8250000000000001	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	32	0.8	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	31	0.775	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	31	0.775	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	31	0.775	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	29	0.7250000000000001	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	29	0.7250000000000001	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	29	0.7250000000000001	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	28	0.7000000000000001	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	28	0.7000000000000001	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	28	0.7000000000000001	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	27	0.675	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	26	0.65	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	26	0.65	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	25	0.625	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	25	0.625	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	25	0.625	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	24	0.6	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	23	0.575	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	23	0.575	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	23	0.575	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	22	0.5499999999999999	No Hit
TTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAA	22	0.5499999999999999	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	21	0.525	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	21	0.525	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	20	0.5	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGT	20	0.5	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	20	0.5	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	20	0.5	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	19	0.475	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	19	0.475	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	19	0.475	No Hit
GCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGG	18	0.44999999999999996	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	18	0.44999999999999996	No Hit
GGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	18	0.44999999999999996	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	18	0.44999999999999996	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	18	0.44999999999999996	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	18	0.44999999999999996	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	18	0.44999999999999996	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	17	0.42500000000000004	No Hit
GCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTA	17	0.42500000000000004	No Hit
TTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCT	16	0.4	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	15	0.375	No Hit
AGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTC	15	0.375	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	15	0.375	No Hit
ATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGA	15	0.375	No Hit
TTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAA	14	0.35000000000000003	No Hit
CTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTC	14	0.35000000000000003	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	14	0.35000000000000003	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	14	0.35000000000000003	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	14	0.35000000000000003	No Hit
TGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTC	14	0.35000000000000003	No Hit
ATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAA	14	0.35000000000000003	No Hit
GGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGT	14	0.35000000000000003	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	14	0.35000000000000003	No Hit
GTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCA	13	0.325	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	13	0.325	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	13	0.325	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	13	0.325	No Hit
TGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAG	13	0.325	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	13	0.325	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	13	0.325	No Hit
GATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATT	12	0.3	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	12	0.3	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	12	0.3	No Hit
CAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCT	12	0.3	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	12	0.3	No Hit
CGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTC	12	0.3	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	12	0.3	No Hit
TACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGC	12	0.3	No Hit
CTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGC	12	0.3	No Hit
TATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGA	12	0.3	No Hit
TAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAA	12	0.3	No Hit
CCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCT	12	0.3	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	12	0.3	No Hit
TATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAA	11	0.27499999999999997	No Hit
CCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGA	11	0.27499999999999997	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	11	0.27499999999999997	No Hit
CTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGAT	11	0.27499999999999997	No Hit
AAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGAC	11	0.27499999999999997	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	11	0.27499999999999997	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	11	0.27499999999999997	No Hit
GAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAA	11	0.27499999999999997	No Hit
GAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTG	11	0.27499999999999997	No Hit
CGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCC	11	0.27499999999999997	No Hit
GGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCT	11	0.27499999999999997	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	11	0.27499999999999997	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	11	0.27499999999999997	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	11	0.27499999999999997	No Hit
GCTATGGCTGGGTTGCGGCCCATCTGTGAATTTATGACCTTCAATTTCTC	10	0.25	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	10	0.25	No Hit
TGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCT	10	0.25	No Hit
TTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAAT	10	0.25	No Hit
CTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTAC	10	0.25	No Hit
GCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGG	10	0.25	No Hit
TTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAA	10	0.25	No Hit
CAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACA	10	0.25	No Hit
CTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACT	10	0.25	No Hit
ACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATG	10	0.25	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	9	0.22499999999999998	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	9	0.22499999999999998	No Hit
TGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATA	9	0.22499999999999998	No Hit
AGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCT	9	0.22499999999999998	No Hit
AGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTGGA	9	0.22499999999999998	No Hit
AGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTT	9	0.22499999999999998	No Hit
TCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTT	9	0.22499999999999998	No Hit
CAAGAAGTCTAGGGCACTTGATGCACTCTTCTGACTTCCATGGATTTTCG	9	0.22499999999999998	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	9	0.22499999999999998	No Hit
GAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGC	9	0.22499999999999998	No Hit
TAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTC	9	0.22499999999999998	No Hit
GCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTG	9	0.22499999999999998	No Hit
CTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTA	9	0.22499999999999998	No Hit
CGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCT	8	0.2	No Hit
GCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTG	8	0.2	No Hit
GCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTG	8	0.2	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	8	0.2	No Hit
TGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATG	8	0.2	No Hit
TCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTAC	8	0.2	No Hit
CTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCC	8	0.2	No Hit
CCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGA	8	0.2	No Hit
AATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCC	8	0.2	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	8	0.2	No Hit
TGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCT	8	0.2	No Hit
TTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCT	8	0.2	No Hit
GCAGCTATGGCTGGGTTGCGGCCCATCTGTGAATTTATGACCTTCAATTT	7	0.17500000000000002	No Hit
TGAGAATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCA	7	0.17500000000000002	No Hit
TGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGG	7	0.17500000000000002	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	7	0.17500000000000002	No Hit
CTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGAT	7	0.17500000000000002	No Hit
AATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACC	7	0.17500000000000002	No Hit
CTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAA	7	0.17500000000000002	No Hit
AATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCG	7	0.17500000000000002	No Hit
GGATTTTCGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTG	7	0.17500000000000002	No Hit
TGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATG	7	0.17500000000000002	No Hit
AAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCG	7	0.17500000000000002	No Hit
ACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCA	7	0.17500000000000002	No Hit
GCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGC	7	0.17500000000000002	No Hit
CTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCAC	7	0.17500000000000002	No Hit
GTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAA	7	0.17500000000000002	No Hit
GTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTG	7	0.17500000000000002	No Hit
ATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGA	7	0.17500000000000002	No Hit
CTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTTGTGAG	7	0.17500000000000002	No Hit
AATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAA	7	0.17500000000000002	No Hit
CTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAA	7	0.17500000000000002	No Hit
CTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGG	7	0.17500000000000002	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	6	0.15	No Hit
TCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATG	6	0.15	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	6	0.15	No Hit
CCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTC	6	0.15	No Hit
CTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCC	6	0.15	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	6	0.15	No Hit
ATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAG	6	0.15	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGCACTC	6	0.15	No Hit
CAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTG	6	0.15	No Hit
TGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTT	6	0.15	No Hit
CTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTC	6	0.15	No Hit
CGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTGAGAAAAG	6	0.15	No Hit
ATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAG	6	0.15	No Hit
CCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGG	6	0.15	No Hit
GCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAACCGG	6	0.15	No Hit
TCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGAACTG	6	0.15	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	6	0.15	No Hit
TGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACG	6	0.15	No Hit
GAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCT	6	0.15	No Hit
AGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAA	5	0.125	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	5	0.125	No Hit
CGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACT	5	0.125	No Hit
AAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAA	5	0.125	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGA	5	0.125	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	5	0.125	No Hit
CAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCC	5	0.125	No Hit
AAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCTGA	5	0.125	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGGGCACTTGATGCA	5	0.125	No Hit
AAATAATAAAAAAGCCATTGAGAAAAGACATCTGACCAGCATCTGGCCTC	5	0.125	No Hit
ATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTC	5	0.125	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	5	0.125	No Hit
TGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAA	5	0.125	No Hit
CATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCA	5	0.125	No Hit
AGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTG	5	0.125	No Hit
TCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTG	5	0.125	No Hit
CTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGG	5	0.125	No Hit
TCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAA	5	0.125	No Hit
CTGGAACTTGTTCTGTAGACCAGGCTGGCTTTGAACTCACACAGTTCCGC	5	0.125	No Hit
TTTTCGGCAAGATACTCAATACACATAAAATAATAAAAAAGCCATTGAGA	5	0.125	No Hit
TTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTA	5	0.125	No Hit
CCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCC	5	0.125	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	5	0.125	No Hit
GGCGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGC	5	0.125	No Hit
AGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATG	5	0.125	No Hit
ATCTTCTCAAGTATTACAGCTCCTGAACTGCCTTCTACTAAATCAGACTT	5	0.125	No Hit
CCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAAC	5	0.125	No Hit
GTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2026-05-21T16:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
Read 17858514 spots for SRR25656854.sra
Written 17858514 spots for SRR25656854.sra
SRR ids: ['SRR25656854.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_v17sl5hc
SRR25656854.sra spots: 142868112
blocks: [[1, 17858514], [17858515, 35717028], [35717029, 53575542], [53575543, 71434056], [71434057, 89292570], [89292571, 107151084], [107151085, 125009598], [125009599, 142868112]]
SRR25656854 file size 34386712
SRR25656854 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25656854 SRR25656854_1.fastq SRR25656854_2.fastq
Input file:	SRR25656854_1.fastq
Paired file:	SRR25656854_2.fastq
trimmed:	SRR25656854-trimmed-pair1.fastq, SRR25656854-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 21 16:42:43 2026 >> started

Thu May 21 16:49:35 2026 >> done (411.849s)
142868112 read pairs processed; of these:
        0 ( 0.00%) short read pairs filtered out after trimming by size control
      279 ( 0.00%) empty read pairs filtered out after trimming by size control
142867833 (100.00%) read pairs available; of these:
    42202 ( 0.03%) trimmed read pairs available after processing
142825631 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 20	       44	  0.00%
 21	       49	  0.00%
 22	       41	  0.00%
 23	       69	  0.00%
 24	       58	  0.00%
 25	       81	  0.00%
 26	      109	  0.00%
 27	      163	  0.00%
 28	      150	  0.00%
 29	      208	  0.00%
 30	      252	  0.00%
 31	      326	  0.00%
 32	      373	  0.00%
 33	      508	  0.00%
 34	      498	  0.00%
 35	      564	  0.00%
 36	      687	  0.00%
 37	      706	  0.00%
 38	      856	  0.00%
 39	     1077	  0.00%
 40	     1251	  0.00%
 41	     1581	  0.00%
 42	     1670	  0.00%
 43	     1749	  0.00%
 44	     1827	  0.00%
 45	     2199	  0.00%
 46	     2383	  0.00%
 47	     2994	  0.00%
 48	     3384	  0.00%
 49	     4340	  0.00%
 50	     4476	  0.00%
 51	     4909	  0.00%
 52	     5451	  0.00%
 53	     6064	  0.00%
 54	     6342	  0.00%
 55	     6810	  0.00%
 56	     7548	  0.01%
 57	     8343	  0.01%
 58	     9801	  0.01%
 59	    13590	  0.01%
 60	    41180	  0.03%
 61	    53317	  0.04%
 62	    44394	  0.03%
 63	    44727	  0.03%
 64	    36347	  0.03%
 65	    38387	  0.03%
 66	    37436	  0.03%
 67	    42822	  0.03%
 68	    37699	  0.03%
 69	    41808	  0.03%
 70	    50013	  0.04%
 71	    49504	  0.03%
 72	    47224	  0.03%
 73	    50435	  0.04%
 74	    54796	  0.04%
 75	    60014	  0.04%
 76	    66316	  0.05%
 77	    71830	  0.05%
 78	    77118	  0.05%
 79	    84963	  0.06%
 80	    95293	  0.07%
 81	   105180	  0.07%
 82	   116200	  0.08%
 83	   126208	  0.09%
 84	   135072	  0.09%
 85	   153286	  0.11%
 86	   167345	  0.12%
 87	   181665	  0.13%
 88	   189774	  0.13%
 89	   204243	  0.14%
 90	   229479	  0.16%
 91	   258602	  0.18%
 92	   274006	  0.19%
 93	   304797	  0.21%
 94	   343442	  0.24%
 95	   373051	  0.26%
 96	   431716	  0.30%
 97	   556722	  0.39%
 98	  1757260	  1.23%
 99	 12112291	  8.48%
100	 62891199	 44.02%
101	 60727141	 42.51%
142867833 reads passed initial QC


criterion=sequence-density
sequence-density=1.00
sequence-density-rank=1
fanout-score=2.99
fanout-score-rank=23
prefix-density=0.99
prefix-fanout=3.0
sequence=ACCTGGTTCACC


criterion=fanout-score
sequence-density=0.65
sequence-density-rank=15
fanout-score=31.75
fanout-score-rank=1
prefix-density=2.43
prefix-fanout=8.5
sequence=TTCACCCTTTGG


criterion=sequence-density
sequence-density=2.27
sequence-density-rank=1
fanout-score=7.31
fanout-score-rank=7
prefix-density=4.69
prefix-fanout=3.5
sequence=AAGAAAAAGGTTCC


criterion=fanout-score
sequence-density=0.63
sequence-density-rank=18
fanout-score=17.23
fanout-score-rank=1
prefix-density=1.86
prefix-fanout=5.8
sequence=CCAGGTCCTGCAGG
SRR25656854 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 16:49:55
                             Started mapping on |	May 21 16:49:56
                                    Finished on |	May 21 16:57:49
       Mapping speed, Million of reads per hour |	1087.37

                          Number of input reads |	142867833
                      Average input read length |	199
                                    UNIQUE READS:
                   Uniquely mapped reads number |	117637068
                        Uniquely mapped reads % |	82.34%
                          Average mapped length |	199.43
                       Number of splices: Total |	89430187
            Number of splices: Annotated (sjdb) |	88933108
                       Number of splices: GT/AG |	88336677
                       Number of splices: GC/AG |	909731
                       Number of splices: AT/AC |	87846
               Number of splices: Non-canonical |	95933
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.01%
                        Deletion average length |	2.92
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.64
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	17819302
             % of reads mapped to multiple loci |	12.47%
        Number of reads mapped to too many loci |	789127
             % of reads mapped to too many loci |	0.55%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.56%
                     % of reads unmapped: other |	0.07%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	7631480	7631480	7631480
N_multimapping	17819302	17819302	17819302
N_noFeature	7665417	111884056	8414558
N_ambiguous	7150667	77020	2287196
UnstrandedReadsAssigned:102820984 PositiveStrandReadsAssigned:5675992 NegativeStrandReadsAssigned:106935314
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=100 echo kmer=95
SRR25656854 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25656854-trimmed-pair1.fastq
                             SRR25656854-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 142,867,833 reads, 108,655,136 reads pseudoaligned
[quant] estimated average fragment length: 192.822
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,284 rounds

 109283 SRR25656854.ke.tsv
  52636 SRR25656854.se.tsv
 161919 total
==> SRR25656854.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25656854.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	5
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25656854 completed mapping pipeline successfully
