Starting /dee2/code/volunteer_pipeline.sh SRR25656852
    current disk space = 464528768000
    free memory = 395442088 
SRR25656852 SRAfilesize
c2e808a1262eccc12e4057cbc46a55ec  SRR25656852.sra
SRR25656852.sra file validated
SRR25656852 is paired end
SRR25656852 is conventional basespace
SRR25656852 read1 length is 39-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6655	37.0	37.0	37.0	37.0	37.0
2	36.44875	37.0	37.0	37.0	37.0	37.0
3	36.6025	37.0	37.0	37.0	37.0	37.0
4	36.601	37.0	37.0	37.0	37.0	37.0
5	36.6995	37.0	37.0	37.0	37.0	37.0
6	36.637	37.0	37.0	37.0	37.0	37.0
7	36.486	37.0	37.0	37.0	37.0	37.0
8	36.53325	37.0	37.0	37.0	37.0	37.0
9	36.5825	37.0	37.0	37.0	37.0	37.0
10-11	36.604	37.0	37.0	37.0	37.0	37.0
12-13	36.621750000000006	37.0	37.0	37.0	37.0	37.0
14-15	36.5795	37.0	37.0	37.0	37.0	37.0
16-17	36.502250000000004	37.0	37.0	37.0	37.0	37.0
18-19	36.586	37.0	37.0	37.0	37.0	37.0
20-21	36.598	37.0	37.0	37.0	37.0	37.0
22-23	36.594750000000005	37.0	37.0	37.0	37.0	37.0
24-25	36.54125	37.0	37.0	37.0	37.0	37.0
26-27	36.52725	37.0	37.0	37.0	37.0	37.0
28-29	36.5085	37.0	37.0	37.0	37.0	37.0
30-31	36.504999999999995	37.0	37.0	37.0	37.0	37.0
32-33	36.4975	37.0	37.0	37.0	37.0	37.0
34-35	36.512249999999995	37.0	37.0	37.0	37.0	37.0
36-37	36.442	37.0	37.0	37.0	37.0	37.0
38-39	36.5	37.0	37.0	37.0	37.0	37.0
40-41	36.521630407601904	37.0	37.0	37.0	37.0	37.0
42-43	36.38734683670918	37.0	37.0	37.0	37.0	37.0
44-45	36.485371342835705	37.0	37.0	37.0	37.0	37.0
46-47	36.456864216054015	37.0	37.0	37.0	37.0	37.0
48-49	36.45511377844461	37.0	37.0	37.0	37.0	37.0
50-51	36.509127281820454	37.0	37.0	37.0	37.0	37.0
52-53	36.436359089772445	37.0	37.0	37.0	37.0	37.0
54-55	36.448112028007	37.0	37.0	37.0	37.0	37.0
56-57	36.42210552638159	37.0	37.0	37.0	37.0	37.0
58-59	36.352588147036755	37.0	37.0	37.0	37.0	37.0
60-61	36.418854713678414	37.0	37.0	37.0	37.0	37.0
62-63	36.30207551887972	37.0	37.0	37.0	37.0	37.0
64-65	36.27063531765883	37.0	37.0	37.0	37.0	37.0
66-67	36.37310392749539	37.0	37.0	37.0	37.0	37.0
68-69	36.30739220581603	37.0	37.0	37.0	37.0	37.0
70-71	36.32841051314143	37.0	37.0	37.0	37.0	37.0
72-73	36.32823127457924	37.0	37.0	37.0	37.0	37.0
74-75	36.3140350877193	37.0	37.0	37.0	37.0	37.0
76-77	36.26156573434086	37.0	37.0	37.0	37.0	37.0
78-79	36.34371665960876	37.0	37.0	37.0	37.0	37.0
80-81	36.32769199336701	37.0	37.0	37.0	37.0	37.0
82-83	36.25224033729606	37.0	37.0	37.0	37.0	37.0
84-85	36.313178346993325	37.0	37.0	37.0	37.0	37.0
86-87	36.31399646689815	37.0	37.0	37.0	37.0	37.0
88-89	36.319345413931806	37.0	37.0	37.0	37.0	37.0
90-91	36.240373594265265	37.0	37.0	37.0	37.0	37.0
92-93	36.27141556387986	37.0	37.0	37.0	37.0	37.0
94-95	36.23488745982833	37.0	37.0	37.0	37.0	37.0
96-97	36.3350791097382	37.0	37.0	37.0	37.0	37.0
98-99	36.32941919805934	37.0	37.0	37.0	37.0	37.0
100-101	36.47478935004559	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	1.0
25	1.0
26	4.0
27	6.0
28	8.0
29	24.0
30	19.0
31	32.0
32	41.0
33	56.0
34	90.0
35	199.0
36	2299.0
37	1219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.275	8.774999999999999	12.7	45.25
2	20.851063829787233	15.919899874843555	33.942428035043804	29.28660826032541
3	21.85	19.7	23.875	34.575
4	24.275	31.4	22.55	21.775
5	27.625	30.375000000000004	23.65	18.35
6	23.9	34.225	21.025	20.849999999999998
7	13.950000000000001	24.025	42.65	19.375
8	17.57939484871218	26.281570392598148	30.03250812703176	26.106526631657918
9	17.150000000000002	22.875	33.475	26.5
10-11	19.7125	34.362500000000004	23.45	22.475
12-13	20.925	25.374999999999996	28.15	25.55
14-15	22.287499999999998	24.0	25.837500000000002	27.875
16-17	22.8625	25.275	27.287499999999998	24.575
18-19	22.0625	22.375	27.1375	28.425
20-21	17.75	24.462500000000002	29.9375	27.85
22-23	21.6	25.4625	28.449999999999996	24.4875
24-25	22.775000000000002	24.9375	30.5125	21.775
26-27	22.4875	25.35	25.637500000000003	26.525
28-29	20.95	23.25	27.962500000000002	27.8375
30-31	20.75	24.712500000000002	27.537499999999998	27.0
32-33	21.125	28.537499999999998	26.85	23.4875
34-35	22.0125	28.125	27.400000000000002	22.4625
36-37	24.099999999999998	26.8125	26.5	22.5875
38-39	23.875	22.8875	29.062500000000004	24.175
40-41	22.780695173793447	20.967741935483872	29.819954988747188	26.431607901975497
42-43	22.73068267066767	20.967741935483872	31.682920730182545	24.618654663665918
44-45	24.193548387096776	22.355588897224308	29.34483620905226	24.10602650662666
46-47	24.706176544136035	21.680420105026258	30.02000500125031	23.593398349587396
48-49	25.1937984496124	25.03125781445361	25.568892223055762	24.20605151287822
50-51	22.193048262065513	26.71917979494874	24.568642160540136	26.51912978244561
52-53	20.16754188547137	25.64391097774444	26.84421105276319	27.344336084021002
54-55	24.01850462615654	23.218304576144035	29.019754938734682	23.74343585896474
56-57	22.20555138784696	21.10527631907977	33.633408352088026	23.055763940985248
58-59	21.50537634408602	22.9057264316079	29.457364341085274	26.131532883220803
60-61	23.668417104276067	24.306076519129782	28.069517379344838	23.95598899724931
62-63	25.431357839459867	24.93123280820205	25.468867216804203	24.168542135533883
64-65	22.373686843421712	21.435717858929465	31.59079539769885	24.599799899949975
66-67	23.352095059412132	25.203252032520325	26.516572858036273	24.92808005003127
68-69	22.920055048167146	23.620668084574003	27.524083573126486	25.935193294132365
70-71	21.25156445556946	28.335419274092615	27.30913642052566	23.103879849812266
72-73	21.561012277624656	25.206715108995237	29.879729391130045	23.352543222250063
74-75	23.734335839598998	23.24561403508772	28.383458646616543	24.636591478696744
76-77	25.629778167690187	27.158791828549944	25.29138989848352	21.92004010527635
78-79	26.58291457286432	25.23869346733668	26.46984924623116	21.708542713567837
80-81	26.428391643594264	25.081802164611126	26.151522778756608	22.338283413038006
82-83	23.47157443590067	24.719526030505484	29.018025967477623	22.790873566116225
84-85	20.477815699658702	27.43016053596258	32.15775502464922	19.93426873972949
86-87	22.08977935582044	24.397666751204667	28.73446614253107	24.778087750443824
88-89	22.506361323155215	23.52417302798982	30.0381679389313	23.931297709923662
90-91	27.509578544061302	22.03065134099617	28.02043422733078	22.43933588761175
92-93	27.7315229921865	23.030613551940565	26.13039579864224	23.10746765723069
94-95	25.260316236020053	21.763722843553158	29.476796503406604	23.499164417020182
96-97	24.864306022227968	26.092013440165417	27.397260273972602	21.646420263634013
98-99	23.734683004794885	25.772509323388388	28.343100692594565	22.149706979222163
100-101	22.942770064341346	10.802573653911276	33.880799187267186	32.37385709448019
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.0
20	1.0
21	1.0
22	0.5
23	0.0
24	2.5
25	2.5
26	0.0
27	0.5
28	0.5
29	0.0
30	0.5
31	1.5
32	4.0
33	6.0
34	6.5
35	9.5
36	14.0
37	27.5
38	53.0
39	106.5
40	131.5
41	126.5
42	118.5
43	184.0
44	317.0
45	281.0
46	312.5
47	362.0
48	312.5
49	236.0
50	161.5
51	205.5
52	214.0
53	165.0
54	94.5
55	66.0
56	129.5
57	116.5
58	60.5
59	52.5
60	48.0
61	41.5
62	14.0
63	5.0
64	3.0
65	4.5
66	3.0
67	1.5
68	3.0
69	5.0
70	3.0
71	0.5
72	0.5
73	0.5
74	0.0
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.025
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
38-39	1.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.0
64-65	0.0
66-67	1.0
68-69	2.0
70-71	3.0
72-73	2.0
74-75	0.0
76-77	8.0
78-79	7.0
80-81	8.0
82-83	5.0
84-85	17.0
86-87	10.0
88-89	17.0
90-91	12.0
92-93	13.0
94-95	15.0
96-97	43.0
98-99	430.0
100-101	3405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.55
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.46481178396072	17.25
2	14.566284779050736	8.9
3	6.219312602291326	5.7
4	5.564648117839607	6.800000000000001
5	2.7004909983633385	4.125
6	2.2913256955810146	4.2
7	1.6366612111292964	3.5000000000000004
8	1.3911620294599019	3.4000000000000004
9	1.5548281505728314	4.275
>10	7.446808510638298	38.675
>50	0.16366612111292964	3.175
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	69	1.725	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	58	1.4500000000000002	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	49	1.225	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	49	1.225	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	43	1.075	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	39	0.975	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	37	0.9249999999999999	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	35	0.8750000000000001	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	33	0.8250000000000001	No Hit
GTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTC	30	0.75	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	28	0.7000000000000001	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	27	0.675	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	26	0.65	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	25	0.625	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	25	0.625	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	25	0.625	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	23	0.575	No Hit
CAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATGT	23	0.575	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	23	0.575	No Hit
CTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCG	22	0.5499999999999999	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	21	0.525	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	21	0.525	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	21	0.525	No Hit
CCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTT	21	0.525	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	20	0.5	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	19	0.475	No Hit
CTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTGGGCTT	19	0.475	No Hit
CCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCAC	19	0.475	No Hit
CTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCAT	18	0.44999999999999996	No Hit
GTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGG	18	0.44999999999999996	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	18	0.44999999999999996	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	18	0.44999999999999996	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	18	0.44999999999999996	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	17	0.42500000000000004	No Hit
GCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGAT	17	0.42500000000000004	No Hit
GCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACA	16	0.4	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	16	0.4	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	16	0.4	No Hit
CTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTC	16	0.4	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	16	0.4	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	15	0.375	No Hit
CTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAA	15	0.375	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	15	0.375	No Hit
TCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCAC	15	0.375	No Hit
GAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTA	14	0.35000000000000003	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	14	0.35000000000000003	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	14	0.35000000000000003	No Hit
GCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTC	13	0.325	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	13	0.325	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	13	0.325	No Hit
CTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTA	13	0.325	No Hit
CTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTC	13	0.325	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	13	0.325	No Hit
CAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTG	13	0.325	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	13	0.325	No Hit
GTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGC	13	0.325	No Hit
CCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTC	13	0.325	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	13	0.325	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	12	0.3	No Hit
CCAGAGGGTTGTCTGGGTTCCAGAGGTCATAGAAGGGTCGCTCAATGATG	12	0.3	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	12	0.3	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	12	0.3	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	12	0.3	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	12	0.3	No Hit
CCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTA	12	0.3	No Hit
CTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGAT	12	0.3	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	12	0.3	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	11	0.27499999999999997	No Hit
TGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTT	11	0.27499999999999997	No Hit
CCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAA	11	0.27499999999999997	No Hit
CCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCC	11	0.27499999999999997	No Hit
CTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGACTG	11	0.27499999999999997	No Hit
CAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCC	11	0.27499999999999997	No Hit
GGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCT	11	0.27499999999999997	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	10	0.25	No Hit
AGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAA	10	0.25	No Hit
GCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACAC	10	0.25	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	10	0.25	No Hit
CTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTC	10	0.25	No Hit
GGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATG	10	0.25	No Hit
ATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAA	10	0.25	No Hit
CCTACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAG	10	0.25	No Hit
TTCGTATGTAAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGG	10	0.25	No Hit
CCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGA	10	0.25	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	10	0.25	No Hit
TCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGG	10	0.25	No Hit
TGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCA	10	0.25	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	10	0.25	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	10	0.25	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	10	0.25	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	10	0.25	No Hit
ATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTT	10	0.25	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	10	0.25	No Hit
GCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGT	9	0.22499999999999998	No Hit
CCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGG	9	0.22499999999999998	No Hit
ATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATC	9	0.22499999999999998	No Hit
TTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCT	9	0.22499999999999998	No Hit
ATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	9	0.22499999999999998	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	9	0.22499999999999998	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	9	0.22499999999999998	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	9	0.22499999999999998	No Hit
CAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGC	9	0.22499999999999998	No Hit
GGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGT	9	0.22499999999999998	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	9	0.22499999999999998	No Hit
AAAATGGAGTCTGCCCTCCCTCAAAGCCCAGCCTTGGCCGGGTTCCTCTC	9	0.22499999999999998	No Hit
GTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGC	9	0.22499999999999998	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	9	0.22499999999999998	No Hit
CGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATT	9	0.22499999999999998	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	9	0.22499999999999998	No Hit
TTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCT	9	0.22499999999999998	No Hit
TGAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCC	9	0.22499999999999998	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	9	0.22499999999999998	No Hit
GTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAA	8	0.2	No Hit
CCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGT	8	0.2	No Hit
GGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTG	8	0.2	No Hit
GGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGT	8	0.2	No Hit
CTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTC	8	0.2	No Hit
TTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCT	8	0.2	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	8	0.2	No Hit
CTCAAAGCCCAGCCTTGGCCGGGTTCCTCTCTGCCTTTCTCCTTTATGGC	8	0.2	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	8	0.2	No Hit
ATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCT	8	0.2	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	8	0.2	No Hit
CCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCT	8	0.2	No Hit
CTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCAT	8	0.2	No Hit
CTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGA	8	0.2	No Hit
TTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACC	8	0.2	No Hit
GCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAA	8	0.2	No Hit
CAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGG	8	0.2	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	7	0.17500000000000002	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	7	0.17500000000000002	No Hit
CTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGGAA	7	0.17500000000000002	No Hit
CGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACT	7	0.17500000000000002	No Hit
GCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTC	7	0.17500000000000002	No Hit
AGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCC	7	0.17500000000000002	No Hit
CATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATG	7	0.17500000000000002	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	7	0.17500000000000002	No Hit
AAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGG	7	0.17500000000000002	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	7	0.17500000000000002	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	7	0.17500000000000002	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	7	0.17500000000000002	No Hit
GGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATACTGT	7	0.17500000000000002	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	7	0.17500000000000002	No Hit
GCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGG	7	0.17500000000000002	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	7	0.17500000000000002	No Hit
GAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAAC	7	0.17500000000000002	No Hit
GCCACACTTCCTACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTTC	7	0.17500000000000002	No Hit
CTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATA	7	0.17500000000000002	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	7	0.17500000000000002	No Hit
GTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTG	6	0.15	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	6	0.15	No Hit
CTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACA	6	0.15	No Hit
CCTAAATCAATGAGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTG	6	0.15	No Hit
TGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATG	6	0.15	No Hit
GTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAG	6	0.15	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	6	0.15	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	6	0.15	No Hit
GGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGAT	6	0.15	No Hit
ACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	6	0.15	No Hit
GGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCG	6	0.15	No Hit
GTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTT	6	0.15	No Hit
GCCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	6	0.15	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	6	0.15	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	6	0.15	No Hit
AGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTC	6	0.15	No Hit
TGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTG	6	0.15	No Hit
GCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCAC	6	0.15	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	6	0.15	No Hit
GGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCC	6	0.15	No Hit
ATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCT	6	0.15	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	6	0.15	No Hit
CTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATG	6	0.15	No Hit
TGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTAT	6	0.15	No Hit
TGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAA	6	0.15	No Hit
CTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCT	6	0.15	No Hit
TAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTG	6	0.15	No Hit
AGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAAT	6	0.15	No Hit
TGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCC	5	0.125	No Hit
TTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTT	5	0.125	No Hit
CTCGACCTAAATCAATGAGATACTGTAGTCTCCTGAGACTCAAAGGCTGA	5	0.125	No Hit
CTAAATCAATGAGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTGG	5	0.125	No Hit
GGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCA	5	0.125	No Hit
GTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCA	5	0.125	No Hit
TCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGA	5	0.125	No Hit
TCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTA	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
GGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTG	5	0.125	No Hit
GGCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAG	5	0.125	No Hit
ATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGG	5	0.125	No Hit
CTTCCTACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGT	5	0.125	No Hit
CACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGAT	5	0.125	No Hit
TGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCGATC	5	0.125	No Hit
GCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCT	5	0.125	No Hit
CCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGA	5	0.125	No Hit
CTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCG	5	0.125	No Hit
CACACTTCCTACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTTCTG	5	0.125	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	5	0.125	No Hit
TACCTCTTCTCCGATCTCTTGGACGTCTTTCCCGTTTTCTGGAGTTAGGA	5	0.125	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	5	0.125	No Hit
TTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATT	5	0.125	No Hit
GCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGT	5	0.125	No Hit
GGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATT	5	0.125	No Hit
CACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACC	5	0.125	No Hit
GTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGC	5	0.125	No Hit
GCCGGGTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTA	5	0.125	No Hit
CTTTTCTCAATGGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCG	5	0.125	No Hit
CGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTG	5	0.125	No Hit
CCCGTTTTCTGGAGTTAGGACTGGGCTTCAGGTTGGCCAGGCTCACTCTC	5	0.125	No Hit
CTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTG	5	0.125	No Hit
TGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCC	30	0.0051502087	55.404415	94-95
>>END_MODULE
SRR25656852 read2 length is 39-101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25656852_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.23	37.0	37.0	37.0	37.0	37.0
2	36.392	37.0	37.0	37.0	37.0	37.0
3	36.338	37.0	37.0	37.0	37.0	37.0
4	36.5115	37.0	37.0	37.0	37.0	37.0
5	36.482	37.0	37.0	37.0	37.0	37.0
6	36.438	37.0	37.0	37.0	37.0	37.0
7	36.4035	37.0	37.0	37.0	37.0	37.0
8	36.5495	37.0	37.0	37.0	37.0	37.0
9	36.428	37.0	37.0	37.0	37.0	37.0
10-11	36.45625	37.0	37.0	37.0	37.0	37.0
12-13	36.487750000000005	37.0	37.0	37.0	37.0	37.0
14-15	36.41975	37.0	37.0	37.0	37.0	37.0
16-17	36.469750000000005	37.0	37.0	37.0	37.0	37.0
18-19	36.396249999999995	37.0	37.0	37.0	37.0	37.0
20-21	36.445	37.0	37.0	37.0	37.0	37.0
22-23	36.37925	37.0	37.0	37.0	37.0	37.0
24-25	36.39975	37.0	37.0	37.0	37.0	37.0
26-27	36.3695	37.0	37.0	37.0	37.0	37.0
28-29	36.38175	37.0	37.0	37.0	37.0	37.0
30-31	36.3955	37.0	37.0	37.0	37.0	37.0
32-33	36.29425	37.0	37.0	37.0	37.0	37.0
34-35	36.2755	37.0	37.0	37.0	37.0	37.0
36-37	36.28775	37.0	37.0	37.0	37.0	37.0
38-39	36.305499999999995	37.0	37.0	37.0	37.0	37.0
40-41	36.272818204551136	37.0	37.0	37.0	37.0	37.0
42-43	36.09852463115779	37.0	37.0	37.0	37.0	37.0
44-45	36.18529632408102	37.0	37.0	37.0	37.0	37.0
46-47	36.204801200300075	37.0	37.0	37.0	37.0	37.0
48-49	36.18279569892473	37.0	37.0	37.0	37.0	37.0
50-51	36.18367091772943	37.0	37.0	37.0	37.0	37.0
52-53	36.10252563140786	37.0	37.0	37.0	37.0	37.0
54-55	36.22005501375344	37.0	37.0	37.0	37.0	37.0
56-57	36.16304076019004	37.0	37.0	37.0	37.0	37.0
58-59	36.074768692173045	37.0	37.0	37.0	37.0	37.0
60-61	36.13953488372093	37.0	37.0	37.0	37.0	37.0
62-63	36.13153288322081	37.0	37.0	37.0	37.0	37.0
64-65	36.099299649824914	37.0	37.0	37.0	37.0	37.0
66-67	36.107940885629205	37.0	37.0	37.0	37.0	37.0
68-69	36.07342612815467	37.0	37.0	37.0	37.0	37.0
70-71	36.12991239048811	37.0	37.0	37.0	37.0	37.0
72-73	36.03343865129407	37.0	37.0	37.0	37.0	37.0
74-75	35.900526183913804	37.0	37.0	37.0	37.0	37.0
76-77	36.02118224652084	37.0	37.0	37.0	37.0	37.0
78-79	35.9969744497805	37.0	37.0	37.0	37.0	37.0
80-81	36.00729610564041	37.0	37.0	37.0	37.0	37.0
82-83	35.95098949824852	37.0	37.0	37.0	37.0	37.0
84-85	36.071253531849926	37.0	37.0	37.0	37.0	37.0
86-87	35.90431467836711	37.0	37.0	37.0	37.0	37.0
88-89	35.858276747884645	37.0	37.0	37.0	37.0	37.0
90-91	35.8904180730584	37.0	37.0	37.0	37.0	37.0
92-93	35.88794251145906	37.0	37.0	37.0	37.0	37.0
94-95	35.864216602358084	37.0	37.0	37.0	37.0	37.0
96-97	35.824989667998345	37.0	37.0	37.0	37.0	37.0
98-99	35.89390416175105	37.0	37.0	37.0	37.0	37.0
100-101	36.07964709708896	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	3.0
25	1.0
26	4.0
27	13.0
28	13.0
29	22.0
30	23.0
31	43.0
32	51.0
33	72.0
34	152.0
35	384.0
36	2489.0
37	730.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.375	14.249999999999998	13.5	33.875
2	26.825	21.2	26.674999999999997	25.3
3	25.825	25.55	26.85	21.775
4	30.45	30.225	21.075	18.25
5	31.275	34.725	15.925	18.075
6	24.625	33.425	19.675	22.275
7	22.875	20.724999999999998	32.75	23.65
8	24.5	21.349999999999998	24.85	29.299999999999997
9	28.249999999999996	23.0	24.05	24.7
10-11	28.1875	30.562499999999996	17.9	23.35
12-13	25.224999999999998	22.8625	24.8	27.1125
14-15	26.1625	25.137500000000003	24.675	24.025
16-17	27.0875	26.650000000000002	21.8125	24.45
18-19	23.9375	26.3125	24.887500000000003	24.8625
20-21	26.825	27.2625	23.1375	22.775000000000002
22-23	26.75	26.85	21.95	24.45
24-25	23.5	28.4125	24.15	23.9375
26-27	25.15	28.975	21.4125	24.462500000000002
28-29	24.975	30.012499999999996	19.9125	25.1
30-31	23.6125	24.65	28.000000000000004	23.7375
32-33	25.912499999999998	26.5625	23.8625	23.6625
34-35	28.3625	27.55	21.475	22.6125
36-37	24.375	29.575000000000003	26.450000000000003	19.6
38-39	26.375	25.0625	24.962500000000002	23.599999999999998
40-41	23.068267066766694	25.218804701175294	26.70667666916729	25.006251562890725
42-43	26.244061015253813	27.394348587146787	21.817954488622153	24.543635908977244
44-45	27.406851712928233	26.11902975743936	24.01850462615654	22.455613903475868
46-47	24.056014003500874	29.982495623905976	22.405601400350086	23.55588897224306
48-49	23.74343585896474	26.16904226056514	25.79394848712178	24.293573393348336
50-51	21.945729648618233	27.32274602976116	25.89721145429536	24.83431286732525
52-53	24.081020255063766	29.644911227806954	24.643660915228807	21.630407601900476
54-55	25.218804701175294	30.107526881720432	24.381095273818453	20.29257314328582
56-57	23.593398349587396	29.21980495123781	24.63115778944736	22.55563890972743
58-59	24.33108277069267	27.74443610902726	26.669167291822955	21.255313828457115
60-61	26.144036009002253	27.86946736684171	22.218054513628406	23.768442110527634
62-63	22.030507626906726	28.532133033258315	25.218804701175294	24.218554638659665
64-65	24.92496248124062	26.25062531265633	23.62431215607804	25.200100050025014
66-67	26.01626016260163	27.604752970606626	22.088805503439648	24.290181363352094
68-69	22.557237582885026	28.90028775178281	24.10859502064306	24.433879644689103
70-71	24.705882352941178	31.33917396745932	21.239048811013767	22.715894868585732
72-73	25.203557559814605	26.40611299010397	23.31203808092196	25.078291369159462
74-75	21.172638436482085	30.944625407166125	25.294412427962914	22.588323728388875
76-77	24.37037965167272	29.219396065655936	23.593534644781357	22.81668963788999
78-79	25.119316754584275	27.405174579251444	22.8460185882944	24.62949007786988
80-81	22.20125786163522	31.157232704402517	23.28301886792453	23.358490566037734
82-83	24.354290034017893	28.96560413254378	23.182562681113772	23.497543152324557
84-85	21.89513581806696	28.46493998736576	26.00126342387871	23.638660770688567
86-87	24.98415515274433	28.82494612751933	24.439092407149197	21.751806312587146
88-89	22.79318239633681	33.99898244721445	21.16509793945561	22.04273721699313
90-91	22.637895812053117	28.115423901940755	26.200204290091932	23.046475995914196
92-93	22.66047950502707	26.14075792730085	24.529517916988915	26.669244650683165
94-95	25.541293919356928	29.34007519771814	23.713211461169454	21.40541942175548
96-97	24.2566510172144	31.872717788210746	23.095983307250915	20.774647887323944
98-99	25.343388095879344	29.342849447885804	22.04416913546997	23.26959332076488
100-101	23.39703518485444	12.270048222896946	31.005536702982678	33.32737988926594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.0
20	0.5
21	0.5
22	0.0
23	2.0
24	2.0
25	0.0
26	0.0
27	0.0
28	0.5
29	4.0
30	5.5
31	4.5
32	5.5
33	4.0
34	3.5
35	7.0
36	11.0
37	51.0
38	77.5
39	118.0
40	180.5
41	194.0
42	161.0
43	161.0
44	217.5
45	221.5
46	248.0
47	313.0
48	305.5
49	209.0
50	120.5
51	114.0
52	148.5
53	178.0
54	146.0
55	89.5
56	79.5
57	80.0
58	63.0
59	47.5
60	45.0
61	41.0
62	29.0
63	15.0
64	9.0
65	12.5
66	31.5
67	105.0
68	93.5
69	19.0
70	22.0
71	18.5
72	4.0
73	1.0
74	2.0
75	1.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.012503125781445362
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
38-39	1.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.0
64-65	0.0
66-67	1.0
68-69	2.0
70-71	3.0
72-73	1.0
74-75	0.0
76-77	8.0
78-79	6.0
80-81	8.0
82-83	5.0
84-85	17.0
86-87	11.0
88-89	17.0
90-91	35.0
92-93	22.0
94-95	19.0
96-97	76.0
98-99	456.0
100-101	3311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.32591958939264	15.0
2	13.259195893926432	7.75
3	8.554319931565441	7.5
4	5.474764756201882	6.4
5	5.389221556886228	7.875
6	3.165098374679213	5.55
7	2.309666381522669	4.725
8	1.7108639863130881	4.0
9	1.4542343883661248	3.8249999999999997
>10	7.18562874251497	34.625
>50	0.1710863986313088	2.75
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	57	1.425	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	53	1.325	No Hit
CGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGA	34	0.8500000000000001	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	31	0.775	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	31	0.775	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	31	0.775	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	30	0.75	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	28	0.7000000000000001	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	26	0.65	No Hit
GGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTT	26	0.65	No Hit
GGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCT	26	0.65	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	26	0.65	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	24	0.6	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	24	0.6	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	24	0.6	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	24	0.6	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	23	0.575	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	23	0.575	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	22	0.5499999999999999	No Hit
GTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCT	21	0.525	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	20	0.5	No Hit
GCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAG	20	0.5	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	20	0.5	No Hit
GCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAAT	20	0.5	No Hit
CCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATC	19	0.475	No Hit
GAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATTACAGCTCCT	19	0.475	No Hit
CTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGAT	19	0.475	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	18	0.44999999999999996	No Hit
CCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGC	18	0.44999999999999996	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	18	0.44999999999999996	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	18	0.44999999999999996	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	17	0.42500000000000004	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	17	0.42500000000000004	No Hit
GGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGC	16	0.4	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	16	0.4	No Hit
TTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAA	16	0.4	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	16	0.4	No Hit
TGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGC	15	0.375	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	15	0.375	No Hit
CATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA	15	0.375	No Hit
AGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTC	15	0.375	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	15	0.375	No Hit
GCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGT	15	0.375	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	15	0.375	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	14	0.35000000000000003	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	14	0.35000000000000003	No Hit
GTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGG	14	0.35000000000000003	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	14	0.35000000000000003	No Hit
CCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACG	14	0.35000000000000003	No Hit
CAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGA	14	0.35000000000000003	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	14	0.35000000000000003	No Hit
GCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCAT	14	0.35000000000000003	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	14	0.35000000000000003	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	13	0.325	No Hit
GCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCC	13	0.325	No Hit
CCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAG	13	0.325	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	13	0.325	No Hit
CACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGA	13	0.325	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	12	0.3	No Hit
TGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAG	12	0.3	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	12	0.3	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	12	0.3	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	12	0.3	No Hit
CTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCA	12	0.3	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	12	0.3	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	12	0.3	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	12	0.3	No Hit
GGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATC	12	0.3	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	12	0.3	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	11	0.27499999999999997	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	11	0.27499999999999997	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	11	0.27499999999999997	No Hit
GCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCC	11	0.27499999999999997	No Hit
CTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAA	11	0.27499999999999997	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	11	0.27499999999999997	No Hit
GAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACT	10	0.25	No Hit
CCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCC	10	0.25	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	10	0.25	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	10	0.25	No Hit
GTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGG	10	0.25	No Hit
CCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGA	10	0.25	No Hit
GTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAA	10	0.25	No Hit
GTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAG	10	0.25	No Hit
TGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTC	10	0.25	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	10	0.25	No Hit
TGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGA	10	0.25	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	9	0.22499999999999998	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	9	0.22499999999999998	No Hit
TGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTC	9	0.22499999999999998	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	9	0.22499999999999998	No Hit
GGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGG	9	0.22499999999999998	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	9	0.22499999999999998	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	9	0.22499999999999998	No Hit
GGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATG	9	0.22499999999999998	No Hit
TATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAG	9	0.22499999999999998	No Hit
ATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCA	9	0.22499999999999998	No Hit
TTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAA	9	0.22499999999999998	No Hit
GTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCG	9	0.22499999999999998	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	9	0.22499999999999998	No Hit
TGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAG	9	0.22499999999999998	No Hit
GTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGT	9	0.22499999999999998	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	9	0.22499999999999998	No Hit
GTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGG	9	0.22499999999999998	No Hit
CGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCT	8	0.2	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	8	0.2	No Hit
CTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCA	8	0.2	No Hit
TTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAA	8	0.2	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	8	0.2	No Hit
GTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGA	8	0.2	No Hit
GCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCG	8	0.2	No Hit
TGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCT	8	0.2	No Hit
CAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACA	8	0.2	No Hit
GGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGAT	8	0.2	No Hit
CGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGG	8	0.2	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	8	0.2	No Hit
GGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACAT	8	0.2	No Hit
CGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGG	8	0.2	No Hit
TGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTAT	8	0.2	No Hit
TGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCT	8	0.2	No Hit
GAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAG	8	0.2	No Hit
CAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGA	8	0.2	No Hit
TAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAA	8	0.2	No Hit
CCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAG	8	0.2	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	7	0.17500000000000002	No Hit
TATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAAT	7	0.17500000000000002	No Hit
GATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTC	7	0.17500000000000002	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	7	0.17500000000000002	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	7	0.17500000000000002	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	7	0.17500000000000002	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	7	0.17500000000000002	No Hit
GTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACT	7	0.17500000000000002	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	7	0.17500000000000002	No Hit
GCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGC	7	0.17500000000000002	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	7	0.17500000000000002	No Hit
CACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGAC	7	0.17500000000000002	No Hit
CCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGC	7	0.17500000000000002	No Hit
CATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTG	7	0.17500000000000002	No Hit
TGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATG	7	0.17500000000000002	No Hit
GGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGA	7	0.17500000000000002	No Hit
CCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCT	7	0.17500000000000002	No Hit
AGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTT	7	0.17500000000000002	No Hit
CATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGT	7	0.17500000000000002	No Hit
GGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAAT	7	0.17500000000000002	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	7	0.17500000000000002	No Hit
ATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAG	7	0.17500000000000002	No Hit
CGTGAGGCGGCCACAACGCCTCCACATCAAGCCTTCCCAGGTGCCTGCAG	7	0.17500000000000002	No Hit
CCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGC	7	0.17500000000000002	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	7	0.17500000000000002	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	7	0.17500000000000002	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	7	0.17500000000000002	No Hit
CTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGA	6	0.15	No Hit
GAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAG	6	0.15	No Hit
TCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGG	6	0.15	No Hit
TAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGC	6	0.15	No Hit
GCTATGGCTGGGTTGCGGCCCATCTGTGAATTTATGACCTTCAATTTCTC	6	0.15	No Hit
CCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGA	6	0.15	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	6	0.15	No Hit
CTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGT	6	0.15	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAAACCGGGGCTACCTGGCTGACC	6	0.15	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	6	0.15	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	6	0.15	No Hit
CCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAA	6	0.15	No Hit
GGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAA	6	0.15	No Hit
GGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCT	6	0.15	No Hit
GGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTC	6	0.15	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	6	0.15	No Hit
CGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGT	6	0.15	No Hit
ATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATG	6	0.15	No Hit
ATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGA	6	0.15	No Hit
GGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCA	6	0.15	No Hit
GTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCA	6	0.15	No Hit
TGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCC	6	0.15	No Hit
TTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGA	6	0.15	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	6	0.15	No Hit
GTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGC	6	0.15	No Hit
ATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCT	6	0.15	No Hit
ACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCAT	6	0.15	No Hit
ACGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGT	6	0.15	No Hit
GTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTG	6	0.15	No Hit
GCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAG	6	0.15	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	6	0.15	No Hit
CTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCA	6	0.15	No Hit
GAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCA	6	0.15	No Hit
GCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAAC	6	0.15	No Hit
GGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCA	6	0.15	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	6	0.15	No Hit
GAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGA	6	0.15	No Hit
GAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAC	5	0.125	No Hit
GCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGT	5	0.125	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	5	0.125	No Hit
GTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCA	5	0.125	No Hit
GCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTT	5	0.125	No Hit
GGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCC	5	0.125	No Hit
CCCAGGGATGTGCGCAAGGCACAGGCCCGACTCCTTAGCCCTCCCACACC	5	0.125	No Hit
TGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAAT	5	0.125	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAAACCGGGGCTAC	5	0.125	No Hit
CCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAA	5	0.125	No Hit
CCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGA	5	0.125	No Hit
TCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGA	5	0.125	No Hit
TGCTCCTGGCTGGGTAGTGAATATGGCAGATAAGAAAATTCTAAAACCTA	5	0.125	No Hit
GGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCC	5	0.125	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	5	0.125	No Hit
CAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGT	5	0.125	No Hit
CCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGAC	5	0.125	No Hit
AAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTC	5	0.125	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	5	0.125	No Hit
TGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCA	5	0.125	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	5	0.125	No Hit
CTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCC	5	0.125	No Hit
CTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTAC	5	0.125	No Hit
CAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCT	5	0.125	No Hit
GGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAG	5	0.125	No Hit
AATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCG	5	0.125	No Hit
CCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTC	5	0.125	No Hit
CTCAGGCGAAAGGAGGAGTTATGGGAGAAACTGGCAAAGCAGGGCGAACT	5	0.125	No Hit
GTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGC	5	0.125	No Hit
GGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGA	5	0.125	No Hit
TAGAAAATGAACTGATGTATGGAGTTGCATTTGAACTTCCTGCAGAAGCT	5	0.125	No Hit
AAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCG	5	0.125	No Hit
GCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAA	5	0.125	No Hit
GTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGG	5	0.125	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	5	0.125	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	5	0.125	No Hit
GGCCCGACTCCTTAGCCCTCCCACACCAAAGGCCAAACCTGGGCCCCAGG	5	0.125	No Hit
GCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGC	5	0.125	No Hit
CCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGA	5	0.125	No Hit
ATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACC	5	0.125	No Hit
CTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCAC	5	0.125	No Hit
GGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAA	5	0.125	No Hit
GAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGC	5	0.125	No Hit
ACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCT	5	0.125	No Hit
CAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACA	5	0.125	No Hit
GCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGA	5	0.125	No Hit
TTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTA	5	0.125	No Hit
AGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGG	5	0.125	No Hit
AGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTAC	5	0.125	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	5	0.125	No Hit
CTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGT	5	0.125	No Hit
CACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTT	5	0.125	No Hit
CCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAG	5	0.125	No Hit
GGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACA	5	0.125	No Hit
CAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGG	5	0.125	No Hit
CAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGA	5	0.125	No Hit
CGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCT	5	0.125	No Hit
GCTCAGGCGAAAGGAGGAGTTATGGGAGAAACTGGCAAAGCAGGGCGAAC	5	0.125	No Hit
CTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTA	5	0.125	No Hit
GCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATATGGCAGATA	5	0.125	No Hit
GGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGG	5	0.125	No Hit
ACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATG	5	0.125	No Hit
TTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647111 spots for SRR25656852.sra
Written 7647111 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
Read 7647097 spots for SRR25656852.sra
Written 7647097 spots for SRR25656852.sra
SRR ids: ['SRR25656852.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__4a7jf0x
SRR25656852.sra spots: 122353566
blocks: [[1, 7647097], [7647098, 15294194], [15294195, 22941291], [22941292, 30588388], [30588389, 38235485], [38235486, 45882582], [45882583, 53529679], [53529680, 61176776], [61176777, 68823873], [68823874, 76470970], [76470971, 84118067], [84118068, 91765164], [91765165, 99412261], [99412262, 107059358], [107059359, 114706455], [114706456, 122353566]]
SRR25656852 file size 4393642
SRR25656852 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25656852 SRR25656852_1.fastq SRR25656852_2.fastq
Input file:	SRR25656852_1.fastq
Paired file:	SRR25656852_2.fastq
trimmed:	SRR25656852-trimmed-pair1.fastq, SRR25656852-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Thu May 21 16:55:10 2026 >> started

Thu May 21 17:00:22 2026 >> done (312.827s)
122353566 read pairs processed; of these:
        1 ( 0.00%) short read pairs filtered out after trimming by size control
      279 ( 0.00%) empty read pairs filtered out after trimming by size control
122353286 (100.00%) read pairs available; of these:
    37452 ( 0.03%) trimmed read pairs available after processing
122315834 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	        1	  0.00%
 19	        0	  0.00%
 20	       60	  0.00%
 21	       64	  0.00%
 22	       73	  0.00%
 23	       68	  0.00%
 24	       93	  0.00%
 25	      131	  0.00%
 26	      194	  0.00%
 27	      233	  0.00%
 28	      299	  0.00%
 29	      337	  0.00%
 30	      456	  0.00%
 31	      537	  0.00%
 32	      613	  0.00%
 33	      701	  0.00%
 34	      758	  0.00%
 35	      839	  0.00%
 36	      952	  0.00%
 37	     1096	  0.00%
 38	     1231	  0.00%
 39	     1432	  0.00%
 40	     1666	  0.00%
 41	     1999	  0.00%
 42	     2324	  0.00%
 43	     2333	  0.00%
 44	     2504	  0.00%
 45	     2666	  0.00%
 46	     3054	  0.00%
 47	     3764	  0.00%
 48	     4276	  0.00%
 49	     5364	  0.00%
 50	     5154	  0.00%
 51	     5593	  0.00%
 52	     6379	  0.01%
 53	     7022	  0.01%
 54	     7392	  0.01%
 55	     8055	  0.01%
 56	     8559	  0.01%
 57	     9304	  0.01%
 58	    11178	  0.01%
 59	    14353	  0.01%
 60	    39673	  0.03%
 61	    50074	  0.04%
 62	    41551	  0.03%
 63	    42711	  0.03%
 64	    36705	  0.03%
 65	    38972	  0.03%
 66	    37096	  0.03%
 67	    40190	  0.03%
 68	    36087	  0.03%
 69	    39065	  0.03%
 70	    46788	  0.04%
 71	    46697	  0.04%
 72	    46296	  0.04%
 73	    49800	  0.04%
 74	    52388	  0.04%
 75	    56920	  0.05%
 76	    63461	  0.05%
 77	    65890	  0.05%
 78	    73064	  0.06%
 79	    80371	  0.07%
 80	    89065	  0.07%
 81	   101211	  0.08%
 82	   109112	  0.09%
 83	   117621	  0.10%
 84	   126537	  0.10%
 85	   145090	  0.12%
 86	   157397	  0.13%
 87	   168458	  0.14%
 88	   167952	  0.14%
 89	   182542	  0.15%
 90	   211678	  0.17%
 91	   238138	  0.19%
 92	   245409	  0.20%
 93	   276609	  0.23%
 94	   323037	  0.26%
 95	   349792	  0.29%
 96	   400877	  0.33%
 97	   507049	  0.41%
 98	  1378305	  1.13%
 99	  9107633	  7.44%
100	 51136900	 41.79%
101	 55705968	 45.53%
122353286 reads passed initial QC


criterion=sequence-density
sequence-density=4.28
sequence-density-rank=1
fanout-score=3.34
fanout-score-rank=27
prefix-density=14.25
prefix-fanout=1.0
sequence=ATATTGTCCTCAGATAGAGGGCGTATCAGATA


criterion=fanout-score
sequence-density=0.44
sequence-density-rank=17
fanout-score=32.03
fanout-score-rank=1
prefix-density=1.64
prefix-fanout=8.5
sequence=TTCACCCTTTGG


criterion=sequence-density
sequence-density=3.68
sequence-density-rank=1
fanout-score=3.73
fanout-score-rank=25
prefix-density=13.73
prefix-fanout=1.0
sequence=TATCTGATACGCCCTCTATCTGAGGACAATAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=27.66
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=3.9
sequence=AGGCCAGAGCAGAGGGAAGCGAGCGGCTGAGTTTTATGACGGGCCCGGTGCTGAAGGGCAGGGACAACTGATGGTGCTACTCTGAGCTGCTTCTTCCTCTCTCCTCTTTTGCACAAAGAGTCTCATGTCTGATATTTAGACATGATGAGCTTTGTGCAAAGTGGAACCTGGTTTCTTCTCACCCTTCTTCATCCCACTCTTATTTTGGCACAGCAGTCCAACGTAGATGAATTGGGATGCAGCCACCTTGGTCAGTCCTATGAGTCTAGAGATGTCTGGAAGCCAGAACCATGTCAAATATGTGTCTGTGACTCAGGATCTGTCCTTTGCGATGACATAATCTGTGATGAGGAGCCACTAGACTGCCCCAACCCAGAGATCCCATTTGGAGAATGTTGTGCAATATGCCCACAGCCTTCTACACCTGCTCCTGTGCTTCCTGATGGCCATGGACCTCAAGGCCCCAAGGGAGATCCGGGTCCTCCTGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x ATATTGTCCTCAGATAGAGGGCGTATCAGATA -y TATCTGATACGCCCTCTATCTGAGGACAATAT -o SRR25656852 SRR25656852_1.fastq SRR25656852_2.fastq
Input file:	SRR25656852_1.fastq
Paired file:	SRR25656852_2.fastq
trimmed:	SRR25656852-trimmed-pair1.fastq, SRR25656852-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	ATATTGTCCTCAGATAGAGGGCGTATCAGATA
-- paired 3' end adapter sequence (-y):	TATCTGATACGCCCTCTATCTGAGGACAATAT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	16
Thu May 21 17:19:02 2026 >> started

Thu May 21 17:21:39 2026 >> done (157.207s)
73411972 read pairs processed; of these:
       7 ( 0.00%) short read pairs filtered out after trimming by size control
     384 ( 0.00%) empty read pairs filtered out after trimming by size control
73411581 (100.00%) read pairs available; of these:
    6288 ( 0.01%) trimmed read pairs available after processing
73405293 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       1	  0.00%
 19	       0	  0.00%
 20	      43	  0.00%
 21	      47	  0.00%
 22	      49	  0.00%
 23	      43	  0.00%
 24	      61	  0.00%
 25	      80	  0.00%
 26	     120	  0.00%
 27	     135	  0.00%
 28	     177	  0.00%
 29	     198	  0.00%
 30	     290	  0.00%
 31	     323	  0.00%
 32	     363	  0.00%
 33	     435	  0.00%
 34	     467	  0.00%
 35	     511	  0.00%
 36	     583	  0.00%
 37	     662	  0.00%
 38	     712	  0.00%
 39	     871	  0.00%
 40	     995	  0.00%
 41	    1151	  0.00%
 42	    1427	  0.00%
 43	    1390	  0.00%
 44	    1461	  0.00%
 45	    1595	  0.00%
 46	    1802	  0.00%
 47	    2290	  0.00%
 48	    2566	  0.00%
 49	    3073	  0.00%
 50	    3030	  0.00%
 51	    3284	  0.00%
 52	    3692	  0.01%
 53	    4141	  0.01%
 54	    4325	  0.01%
 55	    4772	  0.01%
 56	    5079	  0.01%
 57	    5541	  0.01%
 58	    6554	  0.01%
 59	    8434	  0.01%
 60	   23959	  0.03%
 61	   30198	  0.04%
 62	   25191	  0.03%
 63	   25803	  0.04%
 64	   22149	  0.03%
 65	   23462	  0.03%
 66	   21836	  0.03%
 67	   23799	  0.03%
 68	   21444	  0.03%
 69	   23374	  0.03%
 70	   28413	  0.04%
 71	   28351	  0.04%
 72	   28340	  0.04%
 73	   30086	  0.04%
 74	   31722	  0.04%
 75	   34431	  0.05%
 76	   38501	  0.05%
 77	   39786	  0.05%
 78	   44659	  0.06%
 79	   48766	  0.07%
 80	   54540	  0.07%
 81	   62208	  0.08%
 82	   66584	  0.09%
 83	   71403	  0.10%
 84	   75329	  0.10%
 85	   87466	  0.12%
 86	   95302	  0.13%
 87	  101833	  0.14%
 88	  100769	  0.14%
 89	  109496	  0.15%
 90	  126423	  0.17%
 91	  142971	  0.19%
 92	  147397	  0.20%
 93	  168450	  0.23%
 94	  193407	  0.26%
 95	  213037	  0.29%
 96	  240071	  0.33%
 97	  303335	  0.41%
 98	  826689	  1.13%
 99	 5474309	  7.46%
100	30643947	 41.74%
101	33439572	 45.55%


criterion=sequence-density
sequence-density=1.53
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=35
prefix-density=1.60
prefix-fanout=2.0
sequence=GCTCCGGCGAGGGAGGCATCCGC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=50.15
fanout-score-rank=1
prefix-density=0.31
prefix-fanout=3.8
sequence=AGCACACAGCAGCGAGGCGCAACGTCGGGGAACCGGGAAGCCCCCTGACCCTCCGGGCGAAGCCAGGAGTCCGCGAAGCCGGCGAGAGAAGACCACGCCAACGGGGGTCGGGCAGGAGAGACCGGACAGCGGCCAGCCCCCGGCGAGAAGCGGAGGCGGAGGACGAACCCACCGTGAACTCGGGCGGCGCCATTGCGCGAGAGATGGAAGGACGGAGGAGGGGAGGTCGACGAGCGACTTGAACCCCGCGAGAGGGAGGAACG


criterion=sequence-density
sequence-density=1.14
sequence-density-rank=1
fanout-score=2.08
fanout-score-rank=31
prefix-density=1.16
prefix-fanout=2.0
sequence=GCGGATGCCTCCCTCGCCGGAGC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=28
fanout-score=111.36
fanout-score-rank=1
prefix-density=0.26
prefix-fanout=7.7
sequence=AAGAAAAAGCGAAGGAATTTCGCAGAGTTGAAGGTGAAGCGCCTGAGGAAGAAGTTTGCCCTGAAGACACTTCGAAAGGCAAGGAGGAAGCTCATCAGCGGGGCCACGTGCTGAGTGCGTGTCACTCTTCGGCCCCTGGGAAGGTCTGAGACTTGGGCCTCCCGCGGCCCTAACCGGGCTCTCCCCGAGCGGGGAGGTGAGTTCCCAGAGAGCAGGGCTCTACGCTTGGGCAGACTGGGCAGGAGAAGCCCCGAGTCGGGGCGGATGCCTC
SRR25656852 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 21 17:30:21
                             Started mapping on |	May 21 17:30:21
                                    Finished on |	May 21 17:45:41
       Mapping speed, Million of reads per hour |	478.77

                          Number of input reads |	122352895
                      Average input read length |	199
                                    UNIQUE READS:
                   Uniquely mapped reads number |	83189133
                        Uniquely mapped reads % |	67.99%
                          Average mapped length |	198.55
                       Number of splices: Total |	55688549
            Number of splices: Annotated (sjdb) |	55311461
                       Number of splices: GT/AG |	54836768
                       Number of splices: GC/AG |	693137
                       Number of splices: AT/AC |	42344
               Number of splices: Non-canonical |	116300
                      Mismatch rate per base, % |	0.34%
                         Deletion rate per base |	0.04%
                        Deletion average length |	4.67
                        Insertion rate per base |	0.04%
                       Insertion average length |	2.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	26503755
             % of reads mapped to multiple loci |	21.66%
        Number of reads mapped to too many loci |	1070384
             % of reads mapped to too many loci |	0.87%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.42%
                     % of reads unmapped: other |	0.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	12836701	12836701	12836701
N_multimapping	26503755	26503755	26503755
N_noFeature	11062299	74552427	14080169
N_ambiguous	7590357	328862	2110833
UnstrandedReadsAssigned:64536477 PositiveStrandReadsAssigned:8307844 NegativeStrandReadsAssigned:66998131
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=100 echo kmer=95
SRR25656852 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25656852-trimmed-pair1.fastq
                             SRR25656852-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 122,352,895 reads, 60,894,573 reads pseudoaligned
[quant] estimated average fragment length: 183.3
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,141 rounds

 109283 SRR25656852.ke.tsv
  52636 SRR25656852.se.tsv
 161919 total
==> SRR25656852.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	3.66667	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	1	0	0
ENSMUST00000178862.1	14	2.5	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	3.66667	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	1	0	0

==> SRR25656852.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25656852 completed mapping pipeline successfully
