Starting /dee2/code/volunteer_pipeline.sh SRR25925850
    current disk space = 1201721380
    free memory = 198367968 
SRR25925850 SRAfilesize
7179b0e11ce36f73ca2052aa951c34a0  SRR25925850.sra
SRR25925850.sra file validated
SRR25925850 is paired end
SRR25925850 is conventional basespace
SRR25925850 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25925850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.761	37.0	37.0	37.0	37.0	37.0
2	35.9255	37.0	37.0	37.0	37.0	37.0
3	35.922	37.0	37.0	37.0	37.0	37.0
4	36.0815	37.0	37.0	37.0	37.0	37.0
5	36.105	37.0	37.0	37.0	37.0	37.0
6	35.8575	37.0	37.0	37.0	37.0	37.0
7	35.923	37.0	37.0	37.0	37.0	37.0
8	36.1475	37.0	37.0	37.0	37.0	37.0
9	36.08025	37.0	37.0	37.0	37.0	37.0
10-11	36.208375000000004	37.0	37.0	37.0	37.0	37.0
12-13	36.092749999999995	37.0	37.0	37.0	37.0	37.0
14-15	36.14275	37.0	37.0	37.0	37.0	37.0
16-17	36.024125	37.0	37.0	37.0	37.0	37.0
18-19	36.072625	37.0	37.0	37.0	37.0	37.0
20-21	36.12075	37.0	37.0	37.0	37.0	37.0
22-23	36.13225	37.0	37.0	37.0	37.0	37.0
24-25	36.04325	37.0	37.0	37.0	37.0	37.0
26-27	35.882875	37.0	37.0	37.0	37.0	37.0
28-29	35.9825	37.0	37.0	37.0	37.0	37.0
30-31	36.046	37.0	37.0	37.0	37.0	37.0
32-33	35.8865	37.0	37.0	37.0	37.0	37.0
34-35	35.98425	37.0	37.0	37.0	37.0	37.0
36-37	35.89425	37.0	37.0	37.0	37.0	37.0
38-39	35.912000000000006	37.0	37.0	37.0	37.0	37.0
40-41	35.745999999999995	37.0	37.0	37.0	37.0	37.0
42-43	35.8145	37.0	37.0	37.0	37.0	37.0
44-45	35.813500000000005	37.0	37.0	37.0	37.0	37.0
46-47	35.869749999999996	37.0	37.0	37.0	37.0	37.0
48-49	35.801500000000004	37.0	37.0	37.0	37.0	37.0
50-51	35.715999999999994	37.0	37.0	37.0	37.0	37.0
52-53	35.875	37.0	37.0	37.0	37.0	37.0
54-55	35.620999999999995	37.0	37.0	37.0	37.0	37.0
56-57	35.722750000000005	37.0	37.0	37.0	37.0	37.0
58-59	35.821625	37.0	37.0	37.0	37.0	37.0
60-61	35.8365	37.0	37.0	37.0	37.0	37.0
62-63	35.749375	37.0	37.0	37.0	37.0	37.0
64-65	35.803250000000006	37.0	37.0	37.0	37.0	37.0
66-67	35.8085	37.0	37.0	37.0	37.0	37.0
68-69	35.69175	37.0	37.0	37.0	37.0	37.0
70-71	35.65975	37.0	37.0	37.0	37.0	37.0
72-73	35.68775	37.0	37.0	37.0	37.0	37.0
74-75	35.522999999999996	37.0	37.0	37.0	37.0	37.0
76-77	35.686	37.0	37.0	37.0	37.0	37.0
78-79	35.704375	37.0	37.0	37.0	37.0	37.0
80-81	35.570625	37.0	37.0	37.0	37.0	37.0
82-83	35.61425	37.0	37.0	37.0	37.0	37.0
84-85	35.654624999999996	37.0	37.0	37.0	37.0	37.0
86-87	35.522999999999996	37.0	37.0	37.0	37.0	37.0
88-89	35.662000000000006	37.0	37.0	37.0	37.0	37.0
90-91	35.551	37.0	37.0	37.0	37.0	37.0
92-93	35.501000000000005	37.0	37.0	37.0	37.0	37.0
94-95	35.57325	37.0	37.0	37.0	37.0	37.0
96-97	35.4345	37.0	37.0	37.0	37.0	37.0
98-99	35.336875000000006	37.0	37.0	37.0	37.0	37.0
100-101	35.40775	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	2.0
25	6.0
26	7.0
27	18.0
28	28.0
29	42.0
30	62.0
31	67.0
32	110.0
33	119.0
34	201.0
35	484.0
36	2398.0
37	455.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.84542271135568	13.406703351675839	11.830915457728866	33.91695847923962
2	26.150000000000002	22.775000000000002	32.05	19.025
3	25.95	27.375	27.025	19.650000000000002
4	29.875	37.35	18.075	14.7
5	30.025000000000002	36.15	21.5	12.325
6	22.909364046069104	40.510766149223834	24.3114672008012	12.268402603905859
7	17.75	27.075	40.849999999999994	14.325
8	23.925	27.05	31.35	17.675
9	24.266733517172224	22.68739032338932	34.895963900727	18.149912258711456
10-11	24.765595699462434	38.44230528816102	21.840230028753595	14.951868983622955
12-13	21.885942971485743	29.5647823911956	30.75287643821911	17.79639819909955
14-15	21.95	32.1375	28.475	17.4375
16-17	21.819091705242087	32.265732515951456	27.64919304391342	18.265982734893033
18-19	22.081821593894656	30.851995496059054	26.54822970098836	20.517953209057925
20-21	21.4125	30.5375	28.075	19.975
22-23	21.880470117529384	34.7586896724181	25.481370342585645	17.879469867466867
24-25	23.75	32.300000000000004	24.625	19.325
26-27	22.494682847491553	32.24071062179407	26.873514325034403	18.39109220567997
28-29	23.293323330832706	29.91997999499875	25.968992248062015	20.817704426106527
30-31	22.1055263815954	29.9074768692173	27.969492373093274	20.017504376094024
32-33	22.518129532383096	30.00750187546887	25.881470367591895	21.59289822455614
34-35	21.175	30.525000000000002	28.012500000000003	20.2875
36-37	23.25	29.299999999999997	27.575	19.875
38-39	24.05	30.2875	26.5625	19.1
40-41	23.082706766917294	31.015037593984964	26.403508771929822	19.49874686716792
42-43	22.5875	30.6875	27.800000000000004	18.925
44-45	23.83037277958469	29.98498874155617	27.683262446835126	18.501376032024016
46-47	21.5	32.1625	25.162499999999998	21.175
48-49	22.55	29.425	27.0	21.025
50-51	21.591478696741856	30.413533834586463	27.681704260651628	20.31328320802005
52-53	19.537499999999998	32.6125	26.724999999999998	21.125
54-55	19.992469879518072	31.864959839357432	26.80722891566265	21.335341365461847
56-57	20.6875	31.374999999999996	26.137500000000003	21.8
58-59	21.298961331497935	31.83581529220373	27.706169440620698	19.159053935677637
60-61	21.462500000000002	32.625	25.724999999999998	20.1875
62-63	23.05093229883619	30.972343886872732	26.717557251908396	19.25916656238268
64-65	22.005501375343837	29.21980495123781	26.65666416604151	22.118029507376843
66-67	20.974999999999998	31.525	26.150000000000002	21.349999999999998
68-69	20.485242621310658	30.577788894447224	27.263631815907953	21.673336668334166
70-71	20.9875	28.962500000000002	27.6	22.45
72-73	19.75	31.2375	27.3	21.712500000000002
74-75	22.946920380570855	30.220330495743614	24.937406109163746	21.89534301452178
76-77	22.7625	31.724999999999998	25.2625	20.25
78-79	22.05679969973727	31.990491680220188	25.034405104466405	20.918303515576127
80-81	21.005880145126987	30.314024771675214	26.18541223570624	22.494682847491553
82-83	20.1125	31.7	26.5875	21.6
84-85	21.37585991244528	31.794871794871792	26.00375234521576	20.825515947467167
86-87	21.5625	31.0125	27.9375	19.4875
88-89	21.0125	30.3875	27.725	20.875
90-91	20.424999999999997	31.574999999999996	28.625	19.375
92-93	21.2625	31.0125	26.424999999999997	21.3
94-95	21.349999999999998	29.9625	27.6375	21.05
96-97	20.895895895895897	30.593093093093092	27.32732732732733	21.183683683683686
98-99	21.390106449592988	29.780839073262367	28.954289292423297	19.87476518472135
100-101	19.3125	32.0	25.5375	23.150000000000002
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.5
17	2.5
18	0.0
19	0.5
20	2.0
21	1.5
22	0.5
23	3.0
24	7.5
25	8.5
26	11.5
27	25.5
28	35.5
29	36.5
30	39.5
31	51.5
32	93.5
33	108.0
34	96.0
35	115.5
36	116.5
37	129.0
38	157.5
39	156.5
40	176.5
41	216.5
42	247.5
43	283.0
44	254.5
45	217.5
46	200.0
47	174.0
48	182.0
49	171.0
50	147.5
51	130.0
52	98.0
53	81.0
54	73.0
55	51.5
56	30.0
57	13.0
58	5.0
59	2.0
60	4.5
61	8.5
62	10.5
63	7.0
64	2.0
65	0.0
66	0.0
67	3.0
68	3.5
69	0.5
70	0.5
71	0.5
72	0.0
73	0.0
74	0.0
75	0.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.15
7	0.0
8	0.0
9	0.27499999999999997
10-11	0.0125
12-13	0.05
14-15	0.0
16-17	0.08750000000000001
18-19	0.08750000000000001
20-21	0.0
22-23	0.025
24-25	0.0
26-27	0.08750000000000001
28-29	0.025
30-31	0.025
32-33	0.025
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.25
42-43	0.0
44-45	0.075
46-47	0.0
48-49	0.0
50-51	0.25
52-53	0.0
54-55	0.4
56-57	0.0
58-59	0.11249999999999999
60-61	0.0
62-63	0.11249999999999999
64-65	0.025
66-67	0.0
68-69	0.05
70-71	0.0
72-73	0.0
74-75	0.15
76-77	0.0
78-79	0.08750000000000001
80-81	0.08750000000000001
82-83	0.0
84-85	0.0625
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.1
98-99	0.1875
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.55898566703418	22.475
2	21.389195148842337	19.400000000000002
3	12.954796030871002	17.625
4	7.442116868798236	13.5
5	3.8037486218302092	8.625
6	1.8743109151047408	5.1
7	0.6063947078280044	1.925
8	0.7166482910694598	2.6
9	0.4961411245865491	2.025
>10	1.1576626240352812	6.7250000000000005
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGGCATGGAGTTGTGACTGCTGAATGTTGAGGTCGGGAATGTGGGGGTT	19	0.475	No Hit
GGCCATTTGAGGTACATCTGAAAATATGAGTCAATTTTGCCGGGCAAGCA	18	0.44999999999999996	No Hit
TGTAGATGGTGTTTTAAAATGCTGAACAATCAAACCTTAATTTGTATATT	17	0.42500000000000004	No Hit
CGGGCAAGCAATTAACTGGTTAACTAGTCCTTGATTGCTTTTAAATTTCA	17	0.42500000000000004	No Hit
GTTAAAAAGAATTGCAAAAATGATGTGGGGGAGGGGCAAGAAGTTAGGGG	17	0.42500000000000004	No Hit
CCTGAATATGCTAATGCTTTGCATTAAAATCACATTTTTCTTATGTCAAA	16	0.4	No Hit
CATGGAAGAGCTAAATTCAACCTTATTCTTAAGCCCAGAGGACAAAAGCT	12	0.3	No Hit
GGCACAGTGATACAGAAAATCTAGATAAGATGAGGCTAGAAAGTAAACTT	12	0.3	No Hit
GTACAACTGTAAGCTAAACATGAGTGATCTTGCTCCTAGAGACTTAAATA	12	0.3	No Hit
CAAAGAGATACAGCATTCTGTATAGAGGTTCATGATAAAAACATAGATAC	12	0.3	No Hit
GGAATAACCAGAAAGCACGGAAATGATGGTATACCTCCAAGCCTTTTCTA	11	0.27499999999999997	No Hit
GCTACTTATAAATTTTATACCATTTCTTTGTGCATAAGCTTGAGCCATGT	11	0.27499999999999997	No Hit
CTGGGGACAGGAGGGAGAGATGGGCTAAGTCAGAGAGTGTTTGAGTCAAG	11	0.27499999999999997	No Hit
CAAGAAGTTAGGGGTCTGTCTGGAAGTGAGACTGTGAGTGGGTGACTGAG	11	0.27499999999999997	No Hit
GCAAGAGATAATCGAGCAGAAACTCTGAGGATAAAATAAGGAGAGATTCA	11	0.27499999999999997	No Hit
TGTGTGTGCCCATGTGCCAGAGGAGTTCAGAAAAGAGTGTGAGATCCCTT	11	0.27499999999999997	No Hit
CCTGACCATAAAAATCTTAAACAATATGGAAATCCTGAAAAAAAATAGCA	11	0.27499999999999997	No Hit
GTCCATTGCTAACTACATAGTCAGCCAGATTCTAATCACGTGGGGCTCCC	10	0.25	No Hit
CCAGATTCTAATCACGTGGGGCTCCCTCATCTGTCAGTGAGTTCTACTTG	10	0.25	No Hit
GTTCATTACAGTGTGTTCTCTGCTTACCTCTGCTTGGAAATTTCCCTAAT	10	0.25	No Hit
ATGTGGGGCACTGAGACAATGATCGTGAGCTCAATGGCAGCCTGGGCAAC	10	0.25	No Hit
CGGAAATGATGGTATACCTCCAAGCCTTTTCTATATTCTCTACAAAAATC	9	0.22499999999999998	No Hit
CAGATAGTCACACAGAGACACACAGAAAGACACAGACAGACACAGAGACA	9	0.22499999999999998	No Hit
GTGCTGTTAAAAAGAATTGCAAAAATGATGTGGGGGAGGGGCAAGAAGTT	9	0.22499999999999998	No Hit
TTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTTTCTGTC	9	0.22499999999999998	No Hit
CAGAACTGTAGAAAGGGAGCTGTGTCAAGGTGGGTCGTGAAGGTTGGAGA	9	0.22499999999999998	No Hit
GATGAGATGAGATGAGATGAGATGAGATGAGATGAGATGAGATGAGATGA	9	0.22499999999999998	No Hit
CTTGGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTTTCTGTCTGTGCCT	9	0.22499999999999998	No Hit
GTTTGAATGAATTCCCTCCCCTCTGTAGACTTGTTCCCACACCCCAGGCC	9	0.22499999999999998	No Hit
GCTGGAGTAAGTTTGCTGAAACAATGTAAAACATTTGTGTGAATTTGGAA	9	0.22499999999999998	No Hit
GGAGAGTACAACAGAGTACCAGGAGATGAGATGGTGAGATGAGATGAGAT	8	0.2	No Hit
AAAAAGAATTGCAAAAATGATGTGGGGGAGGGGCAAGAAGTTAGGGGTCT	8	0.2	No Hit
TGGGGGTACAACTGTAAGCTAAACATGAGTGATCTTGCTCCTAGAGACTT	8	0.2	No Hit
CCCCTGACCATAAAAATCTTAAACAATATGGAAATCCTGAAAAAAAATAG	8	0.2	No Hit
GACAGGAGGGAGAGATGGGCTAAGTCAGAGAGTGTTTGAGTCAAGACTTG	8	0.2	No Hit
CGGAGATCTTTCCTAACTGCAGGAGCAGATGTTGACACAGATCGTTATAA	8	0.2	No Hit
GCTGTTTGGATATTTGGATTTTGCATGGCATGACCCCTACTGTCTGAGCA	8	0.2	No Hit
CCTACTCATTAATATAACAGAAAACATGAAACAGTATTCATGTTGAAATT	8	0.2	No Hit
CTGGTTTTGTGTGTCAACTTGACACAGCTGAAGATATCACAGAGAAAGGA	8	0.2	No Hit
CTGGGGATGGGTAGTGTAGCAGGAGGCTCCCAAAAGCTCACCAGCCAACA	8	0.2	No Hit
CCAGTCCCCAGGGAAGGGCTCTTGCTGACATCACATACCTGAAGATTTCT	8	0.2	No Hit
GTCAAAATAGGTGCAGTAAGCTCTGGCCACCTGAAGGTGGTACAAAGAGA	8	0.2	No Hit
GGTGAATTACCCCAAAACAGTATTTAAATTTAGATTACAATAAAGAAGGG	8	0.2	No Hit
GTAATATGCATCCTCTAATCAAGCCTTCTCACCATGCTCAAATTGAAACT	7	0.17500000000000002	No Hit
GACTGAAAGGGGTAAAACCAATTAAAACTATGTGGTTCATATACACTGCT	7	0.17500000000000002	No Hit
GGGTGACTGAGTCAGCTGTATAAAAGCCAGATAGATAGATCTCAGAAAAG	7	0.17500000000000002	No Hit
GGCAGGAGGGTGAAGTGCCTGGTCACTTGCAACCACGGTCACGAAGCAGA	7	0.17500000000000002	No Hit
CGGACACACATACAAAGACAGACTCAGATAGTCACACAGAGACACACAGA	7	0.17500000000000002	No Hit
GGAGATGAGTAGGTCATAATAATAGAGCCTCAATTGATTAGAATAATGAC	7	0.17500000000000002	No Hit
CTCAGCCTCAGCCTTTCCAAGTTACATGTAGGTCAAAGTCACTTAAGAAG	7	0.17500000000000002	No Hit
GCAAGAAGTTAGGGGTCTGTCTGGAAGTGAGACTGTGAGTGGGTGACTGA	7	0.17500000000000002	No Hit
CAGGGTAGAAGTGAGTAGTAAGGAGTTGTGTTGTTTTCATCAGAGTTGTG	7	0.17500000000000002	No Hit
CCTGTTGACTTAATACCATTGCAATGACACTCAGGGTCACCTACATACAT	7	0.17500000000000002	No Hit
GAATTTTTAAACTACTAAGATAATACATCAGGTCATAGATATAATATTTT	7	0.17500000000000002	No Hit
GAGAAACAAACCTTTCAGCAGCACATGGCTCCTTCACCAGGAGGAGGGTG	6	0.15	No Hit
GTGTGTGCCCATGTGCCAGAGGAGTTCAGAAAAGAGTGTGAGATCCCTTG	6	0.15	No Hit
ATGAATAAACCCTTTCCTCCCCAACTTGCTTCTTGGTCATGATGTTTGTG	6	0.15	No Hit
AGACAAGTTGCCTTCCAAATGGTCACCTTTTCCTTCTGCAATAGCACAGC	6	0.15	No Hit
GTGTAATATGCATCCTCTAATCAAGCCTTCTCACCATGCTCAAATTGAAA	6	0.15	No Hit
CCTGGCATGGAGTTGTGACTGCTGAATGTTGAGGTCGGGAATGTGGGGGT	6	0.15	No Hit
TGAGGCAGTAGTAGAGGACAGGTCTCAGTCACAGCTGAGTTCAATCACAG	6	0.15	No Hit
TGCAATTCCAAGAGACAAAAAGCTAAGTCAGGAATCAGGAAGCCAGACTC	6	0.15	No Hit
GTATTACAAAAATTTCTTTTTATTACAGTATCTCTGGTAGTTACTTCTGT	6	0.15	No Hit
CTACCACAACAAACCAAACGCACTTCCTTGTCTTGAAAGGTGGACTGCTG	6	0.15	No Hit
GTTAGGAACAAGGAAGAGCCCTTCATGATGCAAACACCATTAAGAAGCCA	6	0.15	No Hit
GGCACATGTGTATGGTGTGGGCGTGGATTATCTTATATAGATAGCGAGGA	6	0.15	No Hit
GGCTTAGACGGGGTGCAATTCCAAGAGACAAAAAGCTAAGTCAGGAATCA	6	0.15	No Hit
TAAAGGAATGCACCATCATGCCTGGCCTTCTCTTCCGTAATCTTTACTCC	6	0.15	No Hit
GCAGGGAGAGCTAAGAGACAAAAGTGAAAGTTCCATTTTCATGCCAGGTG	6	0.15	No Hit
GAATAGTTTAACACTATTTCAGAAAGAGAGCAGTATCCTCCTTTGAGGCA	6	0.15	No Hit
GGAATCTGTCATGCAAGGGAAATGGAAGGAAGGTCACACAAGGATTACTG	6	0.15	No Hit
GGGGAACAGTGTATCTCACACTATAGCCCGGGCTGGCTTCAAACTCAGAC	6	0.15	No Hit
GGTGAATATAGATTGAAGACCTTATAAGTTGTTGCTTATTGGCTAGAGCA	6	0.15	No Hit
CAGAATTATTAAATTCCTTTTAAAGTTCAAAAACTAAGGGAGGTAAAGTA	6	0.15	No Hit
GGGAAATGAAATACTTAAGTTTTCTTTAAAATTATATTAGTATTAATTTA	6	0.15	No Hit
GTCACTTCTCAGGAGCAACCCATCTTGTTTTTAGAGGCAGTGTCTCTCCC	6	0.15	No Hit
GCTGGGGGTACAACTGTAAGCTAAACATGAGTGATCTTGCTCCTAGAGAC	6	0.15	No Hit
AAATTACTGATGTGAAAACGGATGTTTTACAGCAGGAAATCTCCGCCATT	6	0.15	No Hit
GTTCATATACACTGCTTTAACAGTGAGGAAGTACAAGCTACCAGGATTGC	6	0.15	No Hit
CCCCACTCTATAAGATTCTCCATACTTATCTTTCCCTCTAGACCATTCTT	6	0.15	No Hit
TAGGACAGGAAATTATGTAACTCCATTGAACTAGCCAAATTCAACTCTGA	6	0.15	No Hit
GGCAGGGGTCCGGAGGGGGATTACGAGCCCCTTCGCAATCAGTAATTAGC	6	0.15	No Hit
GGGAATCTGTCATGCAAGGGAAATGGAAGGAAGGTCACACAAGGATTACT	6	0.15	No Hit
CTCAGTTAAAATCTCTTCCATTGCTGACCTGAGCTGCCATCCTTTCCTTC	6	0.15	No Hit
GTGCATTTGATCACAGCTGGAAGGAAACACACATGCTCACTGGGACTCAA	6	0.15	No Hit
ATCAAGATGTGCTCCTTTAACAAATGCCCAGTCCTGACAACATGATAGTC	6	0.15	No Hit
CCACATTGTGTCTGTAGTTGAGGAGCAAAGATCAAGGAATGCATGTTTGT	6	0.15	No Hit
CACAGAAAGACACAGACAGACACAGAGACACACAGACAGATACACAGAGA	6	0.15	No Hit
GGGTTACATGGTTCAAGGCACTACTTATGCCTCAGATTAGAGTCTTCCTC	5	0.125	No Hit
CTTTGATGAGGACCTAGAAGGCAGAAGCTGAATGGAGCAGAGGGAAGATG	5	0.125	No Hit
ATTACAAAAATTTCTTTTTATTACAGTATCTCTGGTAGTTACTTCTGTTC	5	0.125	No Hit
GAATGAGCTGGCTGTAGGAGCCACTCAGCCTCAGCCTTTCCAAGTTACAT	5	0.125	No Hit
CCTAATGTGACCACAGCTTCTCAAGGATACCATCGTGGATCTTCTCCTTA	5	0.125	No Hit
TGGGAATTAAGAGGTTGGGAGGAAAACCTAATTATTTTTTACACATTAGA	5	0.125	No Hit
CCGGGCCTGTCTGTGGTTCACATGCATCCCCGAAATGCTTAGGAGTGACT	5	0.125	No Hit
AGACACACACACACACATAGACACACATAAAGACACATAGACACACAGAC	5	0.125	No Hit
GTAGGTAGGTTAGTATGATCTCATTTTTCAGTGGGAAGCTGATGTTCTGA	5	0.125	No Hit
GTCCAGAATTATTAAATTCCTTTTAAAGTTCAAAAACTAAGGGAGGTAAA	5	0.125	No Hit
CAGAGACACACAGACAGATACACAGAGACACACACAGATACACAGAAAGA	5	0.125	No Hit
GGAAGAAGGAAAGATAAGAAAAGCCAAATAATGTTTGTTTGGTTACTTGT	5	0.125	No Hit
GTACAGTACATATTGCTATTCTTCAGATAAATTAATGGTCTGAGAACTGC	5	0.125	No Hit
AAAAGAATTGCAAAAATGATGTGGGGGAGGGGCAAGAAGTTAGGGGTCTG	5	0.125	No Hit
GCATGGAGTTGTGACTGCTGAATGTTGAGGTCGGGAATGTGGGGGTTCAT	5	0.125	No Hit
GAATAAACAAGAAAAACCCATAGGGAAGCCAAGTAGAAAAAGAAATGGCC	5	0.125	No Hit
GCCTGATGTCACATCCTGAACCACGGCAAACATCTTGCATATCCCCCAAA	5	0.125	No Hit
CACGGACACACATACAAAGACAGACTCAGATAGTCACACAGAGACACACA	5	0.125	No Hit
CTTAGACCTGCCTTTGCAAACTGAGATTATTCATCGTAAGGAAATTACTG	5	0.125	No Hit
CCATGAGTGAGCCTCCCAGGACAGAGAAACGTTTCAAAGCAGGCCCTCAC	5	0.125	No Hit
CCTGGACTGAAAGGGGTAAAACCAATTAAAACTATGTGGTTCATATACAC	5	0.125	No Hit
CCTTAGGCAGCTCAGAAAGAAATAACCTATAAAACCAGACCAGCCTTCCT	5	0.125	No Hit
GAGAAAGCTAATAAACAAACAAACAAATGTGGAAGGCACCCAAAGACCTA	5	0.125	No Hit
TACAACTGTAAGCTAAACATGAGTGATCTTGCTCCTAGAGACTTAAATAG	5	0.125	No Hit
GTTTGTTTGGTTACTTGTTTGCAAATAATTGTTACTTTCCTTAGTCATAA	5	0.125	No Hit
CTCCTGTCCTGATTTCCTTCAGTGATGACCTATGATCTGGTAGTAGAAGT	5	0.125	No Hit
CATGGTTCAAGGCACTACTTATGCCTCAGATTAGAGTCTTCCTCTCTCAC	5	0.125	No Hit
GAGGCAAGAGGATCAGAAGTTCAAGGCCACCCTTAACTACATAGGGCTTT	5	0.125	No Hit
TGTGTGTGTGCCCATGTGCCAGAGGAGTTCAGAAAAGAGTGTGAGATCCC	5	0.125	No Hit
GTGCAATTCCAAGAGACAAAAAGCTAAGTCAGGAATCAGGAAGCCAGACT	5	0.125	No Hit
TGTGGTTACACTCACTTGTAAGGCCAGCTAAAGTGGGAGAATTGTGAGTT	5	0.125	No Hit
GCACAATCTTATAAAACACCAAGACAGCCTTGCCAGGTTACTTTCATGCT	5	0.125	No Hit
TTATTAAATTCCTTTTAAAGTTCAAAAACTAAGGGAGGTAAAGTAACAAA	5	0.125	No Hit
GGAAAGTGGAATCACAGGGGAAGGACAGGCGCAGACAAATTTTATCAAAG	5	0.125	No Hit
GTGAAATCTACAGTTTACTGGCTCTCCAGGAAACTAATTGAAGGTGCTGG	5	0.125	No Hit
GTAAATTACTGATGTGAAAACGGATGTTTTACAGCAGGAAATCTCCGCCA	5	0.125	No Hit
AGAGAAACAAACCTTTCAGCAGCACATGGCTCCTTCACCAGGAGGAGGGT	5	0.125	No Hit
TAAACATGAGTGATCTTGCTCCTAGAGACTTAAATAGACTGAAGGCAGGG	5	0.125	No Hit
GGATATTTGGATTTTGCATGGCATGACCCCTACTGTCTGAGCACCCCTAT	5	0.125	No Hit
CACACCACACACACACACACCACACACACACACCCACACACACACCCACA	5	0.125	No Hit
CTGCAGTCTGCCTGGAGTACACATAGGGCTTTGATGGAGCAGGACTGGAA	5	0.125	No Hit
CAAACCTTTCAGCAGCACATGGCTCCTTCACCAGGAGGAGGGTGGAGCTC	5	0.125	No Hit
GGACAGGAGGGAGAGATGGGCTAAGTCAGAGAGTGTTTGAGTCAAGACTT	5	0.125	No Hit
GTAGAAGTGAGTAGTAAGGAGTTGTGTTGTTTTCATCAGAGTTGTGCAGT	5	0.125	No Hit
GTAGAGGTCAGTTGCTAGCCCTGAACTAATTCAGACCTGTGAGGAAATGT	5	0.125	No Hit
GTGAAGTGAAGCTGCTATGACATCATGTTAGCAATGGCTGCCATGCCCCG	5	0.125	No Hit
TAAAAATCAAGATTAATGTTTTAATATTAAAAGCATTAAATTTTCTGAGG	5	0.125	No Hit
CCATGGATTATGGCCATTTATACCAGGCTGCCACATCCAAAGCAACAAGG	5	0.125	No Hit
GCACACATCTGCCAAAGGAATGAGCTGGCTGTAGGAGCCACTCAGCCTCA	5	0.125	No Hit
GACACTAAGTTAAGGACAACTCTGAGAACAAGCCAAAGATTATGGCCTAA	5	0.125	No Hit
GAGAGTGTTTGAGTCAAGACTTGAGCACCTGCAGTCTGCCTGGAGTACAC	5	0.125	No Hit
AAAATACTTGCCTCACCAAAGCAGCATAGAGGAGAGAAAGGTTTATATAG	5	0.125	No Hit
AAAAGCTTTGGTTCTGAAGATTAACCCATGTGGGTGAGCAGTAGACAGAG	5	0.125	No Hit
CTTAGACGGGGTGCAATTCCAAGAGACAAAAAGCTAAGTCAGGAATCAGG	5	0.125	No Hit
GTTGAGTTTGCAAGTTTACAGCAAGATAGAGACAGGCAGTTTTGTATGAC	5	0.125	No Hit
CGTGAACCATTAAGAGAAAGAAATAAACCATAGCTGGGGTGAAGAACACT	5	0.125	No Hit
GTTACACAGGAACATGTTTTAGAAATCAGGAGGACAGCGGTTCTTTACTA	5	0.125	No Hit
GACATGGTGACATAAGCTGAATAATCCCTTTCCTTTCCATGTTTCTTTGG	5	0.125	No Hit
GGAAGGAAGAAGGAAAGATAAGAAAAGCCAAATAATGTTTGTTTGGTTAC	5	0.125	No Hit
TGAGAGATTGTCATGCTCTTCTGATTCCAGAAGAATATCAGTATGGGTCA	5	0.125	No Hit
CCAGGAATGCATATGGCCTATGTGGTAGTGGGCCTGTGGCTCTAGCTCTC	5	0.125	No Hit
GCAGGATTCTAAAAGGATGGTGAAGACACATGGGTGGGTGAATATAGATT	5	0.125	No Hit
AAAATTTCTTTTTATTACAGTATCTCTGGTAGTTACTTCTGTTCCAAAAT	5	0.125	No Hit
GGAAATTATGTAACTCCATTGAACTAGCCAAATTCAACTCTGAATTCCAC	5	0.125	No Hit
CAGGAAGTGGTGGGGGGAGGGGAAGGAAGAAGGAAAGATAAGAAAAGCCA	5	0.125	No Hit
CACAAATGTCGCTTTCCTACTGAACCATGACCCATGATTACACCTAGAAA	5	0.125	No Hit
GAGGGGGATTACGAGCCCCTTCGCAATCAGTAATTAGCTATTGACAGGTC	5	0.125	No Hit
TCTGTTTTCAGGTGAAACTTGTTCTGACAGGACATTACCCAAGTGGGAAA	5	0.125	No Hit
ATGAACTTAAAGTAGAGGATGGGCAAGCTTCTTTGATCCTTCTCCTATGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0125	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.025	0.0	0.0	0.0	0.0
82-83	0.025	0.0	0.0	0.0	0.0
84-85	0.025	0.0	0.0	0.0	0.0
86-87	0.025	0.0	0.0	0.0	0.0
88-89	0.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR25925850 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25925850_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.7785	37.0	37.0	37.0	37.0	37.0
2	35.609	37.0	37.0	37.0	37.0	37.0
3	35.749	37.0	37.0	37.0	37.0	37.0
4	35.7555	37.0	37.0	37.0	37.0	37.0
5	35.9015	37.0	37.0	37.0	37.0	37.0
6	35.77525	37.0	37.0	37.0	37.0	37.0
7	35.93025	37.0	37.0	37.0	37.0	37.0
8	35.9585	37.0	37.0	37.0	37.0	37.0
9	35.933	37.0	37.0	37.0	37.0	37.0
10-11	36.019999999999996	37.0	37.0	37.0	37.0	37.0
12-13	36.03375	37.0	37.0	37.0	37.0	37.0
14-15	35.89575	37.0	37.0	37.0	37.0	37.0
16-17	36.002250000000004	37.0	37.0	37.0	37.0	37.0
18-19	35.897625	37.0	37.0	37.0	37.0	37.0
20-21	35.776624999999996	37.0	37.0	37.0	37.0	37.0
22-23	35.789625	37.0	37.0	37.0	37.0	37.0
24-25	35.80475	37.0	37.0	37.0	37.0	37.0
26-27	35.780125	37.0	37.0	37.0	37.0	37.0
28-29	35.52725	37.0	37.0	37.0	37.0	37.0
30-31	35.78275	37.0	37.0	37.0	37.0	37.0
32-33	35.687875000000005	37.0	37.0	37.0	37.0	37.0
34-35	35.6945	37.0	37.0	37.0	37.0	37.0
36-37	35.64425	37.0	37.0	37.0	37.0	37.0
38-39	35.705	37.0	37.0	37.0	37.0	37.0
40-41	35.681625	37.0	37.0	37.0	37.0	37.0
42-43	35.618	37.0	37.0	37.0	37.0	37.0
44-45	35.692875	37.0	37.0	37.0	37.0	37.0
46-47	35.62325	37.0	37.0	37.0	37.0	37.0
48-49	35.567375	37.0	37.0	37.0	37.0	37.0
50-51	35.532375	37.0	37.0	37.0	37.0	37.0
52-53	35.61525	37.0	37.0	37.0	37.0	37.0
54-55	35.551249999999996	37.0	37.0	37.0	37.0	37.0
56-57	35.47087500000001	37.0	37.0	37.0	37.0	37.0
58-59	35.481375	37.0	37.0	37.0	37.0	37.0
60-61	35.49325	37.0	37.0	37.0	37.0	37.0
62-63	35.59225	37.0	37.0	37.0	37.0	37.0
64-65	35.376999999999995	37.0	37.0	37.0	37.0	37.0
66-67	35.2975	37.0	37.0	37.0	31.0	37.0
68-69	35.34275	37.0	37.0	37.0	37.0	37.0
70-71	35.226124999999996	37.0	37.0	37.0	25.0	37.0
72-73	35.280875	37.0	37.0	37.0	37.0	37.0
74-75	35.440250000000006	37.0	37.0	37.0	37.0	37.0
76-77	35.349125	37.0	37.0	37.0	37.0	37.0
78-79	35.276875000000004	37.0	37.0	37.0	37.0	37.0
80-81	35.213	37.0	37.0	37.0	25.0	37.0
82-83	35.167625	37.0	37.0	37.0	31.0	37.0
84-85	35.22325	37.0	37.0	37.0	31.0	37.0
86-87	35.207125000000005	37.0	37.0	37.0	31.0	37.0
88-89	35.155375	37.0	37.0	37.0	25.0	37.0
90-91	34.733999999999995	37.0	37.0	37.0	25.0	37.0
92-93	35.15925	37.0	37.0	37.0	25.0	37.0
94-95	35.023250000000004	37.0	37.0	37.0	25.0	37.0
96-97	35.07575	37.0	37.0	37.0	25.0	37.0
98-99	34.870000000000005	37.0	37.0	37.0	25.0	37.0
100-101	34.79675	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	3.0
23	3.0
24	5.0
25	15.0
26	15.0
27	25.0
28	37.0
29	33.0
30	52.0
31	72.0
32	99.0
33	159.0
34	297.0
35	784.0
36	2154.0
37	245.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.675	18.625	16.825000000000003	34.875
2	17.8	26.474999999999998	41.625	14.099999999999998
3	19.975	24.099999999999998	37.325	18.6
4	24.099999999999998	33.550000000000004	23.775	18.575
5	24.975	34.675	22.575	17.775
6	16.9045830202855	37.215126471324815	27.097420485850236	18.782870022539445
7	18.67334167709637	20.32540675844806	40.175219023779725	20.826032540675847
8	19.475	22.95	34.325	23.25
9	20.825	22.225	31.3	25.650000000000002
10-11	21.95	33.3375	24.725	19.9875
12-13	21.748374187093546	25.700350175087543	29.927463731865934	22.623811905952977
14-15	21.04078058543908	27.34550913184889	31.07330497873405	20.540405303977984
16-17	20.4	27.9125	31.7875	19.900000000000002
18-19	21.965245655706962	28.22852856607076	28.678584823102888	21.12764095511939
20-21	20.483285338675348	28.92199824715162	28.98459997495931	21.610116439213723
22-23	20.905792568497436	30.701864131114725	28.162141874139873	20.23020142624797
24-25	19.6875	28.549999999999997	30.8	20.962500000000002
26-27	22.28199674715376	27.586638308519955	30.93957212560991	19.191792818716376
28-29	21.79357450037946	26.865671641791046	31.254743232987604	20.086010624841894
30-31	20.3625	28.599999999999998	30.3	20.7375
32-33	21.436616193217368	28.156676260793397	29.833562758102865	20.57314478788637
34-35	21.29282320580145	26.806701675418854	30.657664416104026	21.24281070267567
36-37	21.7	27.1	31.7625	19.4375
38-39	20.392598149537385	26.831707926981746	31.620405101275317	21.155288822205552
40-41	22.15134459036898	29.318323952470294	29.055659787367105	19.474671669793622
42-43	20.965724293219914	27.307980985739306	30.285213910432823	21.441080810607957
44-45	20.52532833020638	29.018136335209505	30.34396497811132	20.112570356472794
46-47	21.975	29.062500000000004	29.6625	19.3
48-49	21.395523321245467	25.959734900587723	31.5368263098662	21.10791546830061
50-51	22.439024390243905	26.55409631019387	30.456535334584117	20.55034396497811
52-53	22.05	28.037499999999998	30.4625	19.45
54-55	22.33909341347358	26.93463561232156	30.503380916604055	20.2228900576008
56-57	20.948567138030285	27.268176698786135	28.869978726066826	22.913277437116754
58-59	21.288305190744214	25.50343964978111	31.25703564727955	21.951219512195124
60-61	22.218054513628406	26.694173543385848	31.007751937984494	20.080020005001252
62-63	21.525	28.212500000000002	29.7125	20.549999999999997
64-65	21.05526381595399	27.60690172543136	30.357589397349336	20.980245061265315
66-67	21.125	28.0875	30.662499999999998	20.125
68-69	21.498249124562278	28.751875937968986	30.377688844422213	19.372186093046523
70-71	22.31952958838984	26.936069060427876	31.214812961341178	19.52958838984111
72-73	22.042808862185506	26.974590061334336	29.24020528226311	21.74239579421705
74-75	22.5125	28.175	29.1875	20.125
76-77	20.47303216118133	27.593542735577525	31.36028031535477	20.57314478788637
78-79	20.460518082843198	29.670879739707175	29.558253034663995	20.310349142785633
80-81	21.525	27.0	31.974999999999998	19.5
82-83	22.12079529823684	27.38526947605352	29.78616981368013	20.70776541202951
84-85	22.2125	27.5625	30.612499999999997	19.6125
86-87	22.163852407754845	26.691682301438398	31.6072545340838	19.537210756722953
88-89	20.515064383047882	26.703337917239654	29.94124265533192	22.840355044380548
90-91	20.08590197069227	28.271854471955532	29.62354724608388	22.018696311268318
92-93	21.73043260815204	27.694423605901473	30.08252063015754	20.492623155788948
94-95	22.037499999999998	26.825	30.825000000000003	20.3125
96-97	21.7875	27.425	28.175	22.6125
98-99	20.0	26.883604505632043	30.951188986232793	22.165206508135167
100-101	22.380595148787197	26.86921730432608	29.382345586396596	21.36784196049012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	2.0
19	2.5
20	5.0
21	4.5
22	3.0
23	4.5
24	7.0
25	17.5
26	36.0
27	43.5
28	34.0
29	29.0
30	40.5
31	60.0
32	70.5
33	74.5
34	95.5
35	112.5
36	117.5
37	136.0
38	156.0
39	185.0
40	218.5
41	236.5
42	244.0
43	252.5
44	242.5
45	240.0
46	239.5
47	187.5
48	139.5
49	130.0
50	129.0
51	109.5
52	83.0
53	67.0
54	52.0
55	47.0
56	37.0
57	19.0
58	11.5
59	6.0
60	5.0
61	7.0
62	10.0
63	12.5
64	9.0
65	2.0
66	0.5
67	1.5
68	1.5
69	5.5
70	7.0
71	1.5
72	0.0
73	0.0
74	0.0
75	0.5
76	1.5
77	2.5
78	1.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.17500000000000002
7	0.125
8	0.0
9	0.0
10-11	0.0
12-13	0.05
14-15	0.075
16-17	0.0
18-19	0.0125
20-21	0.1625
22-23	0.08750000000000001
24-25	0.0
26-27	0.08750000000000001
28-29	1.175
30-31	0.0
32-33	0.11249999999999999
34-35	0.025
36-37	0.0
38-39	0.025
40-41	0.0625
42-43	0.075
44-45	0.0625
46-47	0.0
48-49	0.0375
50-51	0.0625
52-53	0.0
54-55	0.17500000000000002
56-57	0.11249999999999999
58-59	0.0625
60-61	0.025
62-63	0.0
64-65	0.025
66-67	0.0
68-69	0.05
70-71	0.08750000000000001
72-73	0.13749999999999998
74-75	0.0
76-77	0.11249999999999999
78-79	0.11249999999999999
80-81	0.0
82-83	0.0375
84-85	0.0
86-87	0.0625
88-89	0.0125
90-91	1.05
92-93	0.025
94-95	0.0
96-97	0.0
98-99	0.125
100-101	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.474999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.30621785881253	29.575000000000003
2	22.393641888733054	23.95
3	11.781206171107995	18.9
4	5.189340813464235	11.1
5	3.272557269752221	8.75
6	1.262272089761571	4.05
7	0.2805049088359046	1.05
8	0.09350163627863488	0.4
9	0.23375409069658717	1.125
>10	0.18700327255726976	1.0999999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGAGTTCTAGGTGTCTTTGAAATATACTGTATGATGTGCTGGGAATCA	13	0.325	No Hit
AGTTCTGTGACTCCTGGTCACATTTAAGCCTGCTCTGGACAAGAGATTGC	11	0.27499999999999997	No Hit
CTTGGATGATGTGGGCCAGCCTCTGAGTCTTGGAGATTTAGAGTGATTGG	10	0.25	No Hit
CACCATCAAGGCAAAAGCTTCAGGAACAAAACTGATTTTAACTTACCACA	10	0.25	No Hit
CTGGAACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAAATCTGCCT	9	0.22499999999999998	No Hit
CGAGACAGGGTTTCTTTGTATAGCCCTGGCTGTCCTGGAACTCACTCTGT	9	0.22499999999999998	No Hit
GTCAACTTCTGGAATATTCTGACAAACTTTCTGACTCAAGTAGTAAGGCT	9	0.22499999999999998	No Hit
AGTTCATCCATTTTCCCTCCCCTACTTTAAATTAAAAGTAAATACTAAGA	9	0.22499999999999998	No Hit
CCTAGATCAAACTGAAGAATGTTAAATGGTACAGTAATTTCCTTACGATG	9	0.22499999999999998	No Hit
CTCTGTGACAACGCAGAAGCTAATGAGGAAGGAAGGCTAGAAGCCAGGCA	8	0.2	No Hit
CTTACACATACTGGAAAAATACTGGCTACAAATTCCCTGAACTTTAAACC	8	0.2	No Hit
GAAAGAGTTCTAGGTGTCTTTGAAATATACTGTATGATGTGCTGGGAATC	7	0.17500000000000002	No Hit
ATTGCAAAGAGCTGGATAGTTGAGTTCTTCCATTGCCTCCTCATCAATGG	7	0.17500000000000002	No Hit
GACATACCATAAATTGCTATTTGTTTATTATCTTTCTAAGTTCCTTGGAG	7	0.17500000000000002	No Hit
AAACATTCCTAGTGACAGGCTTGGTTTTGAAATGTATAAAAGATCAAAAT	7	0.17500000000000002	No Hit
GTCTTTGGAAACAAGTAGATTAAAAACAGAAGTCACTCCTAAGCATTTCG	7	0.17500000000000002	No Hit
GTGGGCTTAGTGAGTGCAAGGACCAAGGTTCTGTTCTCAGCCCTTTCCAA	7	0.17500000000000002	No Hit
ATTTAATGCTTTTAATATTAAAACATTAATCTTGATTTTTAAAAGATCTT	6	0.15	No Hit
GCAAAATACTGTAGAAATAACAACAGGGAAAGCTGTTCACATTACAGTCA	6	0.15	No Hit
GTTGTAACTAGAAGGCTTGCAAAGTTATTAAGAGTCACATGAGCATAATT	6	0.15	No Hit
CAGTAGTTCAAGGATAGCCTGGGCTATATGGGAAGATCCTGGCTCAAAAA	6	0.15	No Hit
CAAGAACAAAAGCAGATTTTTAAACTTGCATCTTAAACTTGTAACAAGGC	6	0.15	No Hit
CTCAGTTTGCAAAGGCAGGTCTAAGATGCCTATTTATATTAAAAGAGAAT	6	0.15	No Hit
CTCCGCCCCTTCCTTGAACACTATTAGTGTTTATTACCATCTGTGATCAT	6	0.15	No Hit
TTCTAGAACAGACACTAACAGCCTGGAATATTGTGAGTGGCTGAATAGTG	6	0.15	No Hit
ACAAGAGAGAGCTGAGAGTCACCCTTTTTATTCTCTTGTGAGTAAGCCAT	6	0.15	No Hit
CACCAAAGTGACCCTGTCCATTGTTAGTTATATTGACCTTATCTGTGTAT	6	0.15	No Hit
CTTTGACTAAATCTTAGACCAACACCATCACTGCCATATGTGTAAAGCAC	6	0.15	No Hit
CTGTGATCTAATTGACATACCATAAATTGCTATTTGTTTATTATCTTTCT	6	0.15	No Hit
GTCATTCAGGGAGCACCTAGTATCTATAGAATTTCTCATGAATGTCTCAC	6	0.15	No Hit
TCTCAGTTTCTGGAGTTCGCATACTTTCAAAGAATAGATGAGTTTTGGTT	6	0.15	No Hit
CTTCCTGCATCTATTTCAGAAAGAATGCCCCTAAACTTCAGAGCTGGGCT	6	0.15	No Hit
GAGAAATTGAAATACAAACTAACAGATAACAGTGCCCTCTATTATTTTCA	6	0.15	No Hit
TCTGATAACTCTAACTTATGCCAAGTTGACATAATGCTAGCCAGTGAAGG	6	0.15	No Hit
AATGCCTTACAGTTGGATCTCATGGAGGCATTTCCTCAACTGAAGCTCCT	6	0.15	No Hit
CTCTTTAGAGCCCATTGAGATCAGTTTTTACAGTGCTCTCAGCAAATAGA	6	0.15	No Hit
GTGGACTGTGGTTTGTAACTGATTTCAGGGAGTGGATGGAGTTTGTGGAA	6	0.15	No Hit
CTGGGTTTGGACAGCTTGTTAGTTAGAAAAACACTCATTTGGTGGTATGC	6	0.15	No Hit
GTGAGGAAAAGCAATCCTGGTAGCTTGTACTTCCTCACTGTTAAAGCAGT	6	0.15	No Hit
CTTGCTGCTTCTGGCTTACTGAGATTGGCAATATAGAGATGTATGTAGGT	6	0.15	No Hit
CAATTTTTAAAAAGTCACTACTATATAAAATAATGATATAAAATTAACCA	6	0.15	No Hit
GACTTTGTAGCCTTTGCAGTTCATTGGGTATTACAAGTAATGCAAGACAT	6	0.15	No Hit
TATAATGGCACTCATCTGTAATCGTAGTTCTGGTAAGGCAGAGACAGACA	6	0.15	No Hit
GTTCATGTGTCTTGTCTGGAGAGCTGCTTATAACGATCTGTGTCAACATC	6	0.15	No Hit
GTGTCTATGTGTCTTTATGTGTGTCTATGTGTGTGTGTGTGTCTATGTGT	5	0.125	No Hit
TACATAGTGAGCTTCAGGCTAGCCAAGGCTGCATAGTGAGACTGTGTCTT	5	0.125	No Hit
AGAACCAAGACTACCAGCCCAGAGATGGAGCCACCCACAAGGGGCCTTTC	5	0.125	No Hit
CTTGGTTGGCTCTTGGATGATGTGGGCCAGCCTCTGAGTCTTGGAGATTT	5	0.125	No Hit
CTTACTCTTTGCTGGAAAGTTTACTTTCTAGCCTCATCTTATCTAGATTT	5	0.125	No Hit
ATTTTCCCTTCCTACAGTAAGTGACAATGGACTCTTGGTAGAAATGGCGG	5	0.125	No Hit
AAACAAACAAACAAACAAACAAACAAACCAAGACAACTTTTTTCTTTTTG	5	0.125	No Hit
GTATGATTGTGTGCCTATGTGTATCTGTGTGTCTATGTGTGCCTATGTAT	5	0.125	No Hit
CCTGGCTTGCTCAGCCTGCTCTCTTATAGAACCAAGACTACCAGCCCAGA	5	0.125	No Hit
TGGAAGGTTGTGCATGCTCTGTGCCCACATAAGTCATTCAGGGAGCACCT	5	0.125	No Hit
GTTATTAAGAGTCACATGAGCATAATTTATTTATTAACTAGAAATATGCA	5	0.125	No Hit
CAGGCTCTGAGGTTAACTGAGAGACCCTGACTCAAAGGCTAAAGTGGACT	5	0.125	No Hit
TGGGAATGCAAAAACTTAAAGCAATCCAGGTTTTAGAGCAGCTGAAGGAC	5	0.125	No Hit
TGCGTGTCTGTGTATGTGTATGATTGTGTGCCTATGTGTATCTGTGTGTC	5	0.125	No Hit
GTGGCCTTCAGAAGAATTAAGAGACTTCCTTTAAGGGAAAAAAAAAAAGT	5	0.125	No Hit
CCTAAAGTATCAGAATTCACAACTTGAAGTTTTTCTTATAACTGGAGCTG	5	0.125	No Hit
CATCCATAAAATGAGGGTCCCCACCCATAAAAGGAGGGTCCCTACCCATA	5	0.125	No Hit
AATTCCCAACCTCCAAATTACCATTTTCTATTGTTTTTAAAATTACTGTC	5	0.125	No Hit
TATCTATAGAATTTCTCATGAATGTCTCACTTGGATCTTATTACCTGGGA	5	0.125	No Hit
TTTCTATTGTTTTTAAAATTACTGTCTGCGTTTAGGGTCTCGCTTTGTAG	5	0.125	No Hit
TTCCAGATGTGTTATTTTGCTTGCAGATACTTCACTTTCTGCCTGACACA	5	0.125	No Hit
CTTGAACTCACAATTCTCCCACTTTAGCTGGCCTTACAAGTGAGTGTAAC	5	0.125	No Hit
TATGGAGCCCAGACTGGCCTTGAACTCACAATTCTCCCACTTTAGCTGGC	5	0.125	No Hit
ATTTATTCTACAGTAGTTAACACTTCTATACAAAAGTGATGGGCTAGTGT	5	0.125	No Hit
GAATAAGCCTATCTCCATTCATAAAAGAAAGAATAATAAAAAGGCATTTA	5	0.125	No Hit
AAAATCGTCAACTTCTGGAATATTCTGACAAACTTTCTGACTCAAGTAGT	5	0.125	No Hit
CCAAGTCTATCGTGTCAGAGATCTAAAACCTCTCAACTTTAGCCTTACAC	5	0.125	No Hit
CTGATATCAGAGCAAAGTCCCCTTCTTGTACCCTGTCTGACAGGGAGAGT	5	0.125	No Hit
CTTTAGTAAACTAACAACAGTATCTGATCTATAGCCTGGGTGCTGCAATC	5	0.125	No Hit
GTGGATATTATTGGGTCTCTCTTCACCAAAAAATAATAAAGCTGATTTAT	5	0.125	No Hit
GGAAGGTTGTGCATGCTCTGTGCCCACATAAGTCATTCAGGGAGCACCTA	5	0.125	No Hit
AGGAAAGAGAGTTTCTTGACTTACCAATCCCACTATATCATGGATGCTGA	5	0.125	No Hit
GTTTGGGTGTGTGTATGTTCTGATCTCATCTGCTGTTACCTCTGGCTTAC	5	0.125	No Hit
AGTCATTCAGGGAGCACCTAGTATCTATAGAATTTCTCATGAATGTCTCA	5	0.125	No Hit
AGCAGAGACCCAAACCAAGCAATACAGACTCTAATTTTGGATGTTTTTCT	5	0.125	No Hit
CGTGGTTGCTAGGTCCTTCCTAGAGGAAGAAATGAAAGCGCAAAAGTGTA	5	0.125	No Hit
AGGAGTCTCTGTGACAACGCAGAAGCTAATGAGGAAGGAAGGCTAGAAGC	5	0.125	No Hit
TTCTAGAGCTCCAAAGGCGTGAGCTCCCAGCTGGCTTTGGCTTTCTTCCG	5	0.125	No Hit
GGAAAAGCAATCCTGGTAGCTTGTACTTCCTCACTGTTAAAGCAGTGTAT	5	0.125	No Hit
GTGAGCTCTGAATGCAACCATCCAACTGGTCTAAGTCAGCCTGTGAGGCC	5	0.125	No Hit
CACACACACATATTCTTGCTCTGATATGTTACATTATCCCCTCTCAGTCA	5	0.125	No Hit
TGTAATCCTGCTGCAGACATGATTAGATCTCCAGGCTCTTCAAACTTGGT	5	0.125	No Hit
TGTCCCTCAAGTGCATCTCTGGTGACCTAGTGACTTCCCACTCTGTCCTA	5	0.125	No Hit
GGTCACCTCTCCCTAGGGTATTGCCATAGGTCTAATTTGTCCCCTTGCTT	5	0.125	No Hit
CAAGGTTCTGTTCTCAGCCCTTTCCAAAAAAGCAGTTTCCAGATGTGTTA	5	0.125	No Hit
CTACATAGTGAGCTTCAGGCTAGCCAAGGCTGCATAGTGAGACTGTGTCT	5	0.125	No Hit
CTTCGCTGCCATTGCTGCAAACCTGCCTTCAGAGTTGTTCTGTTCTCCTG	5	0.125	No Hit
CTTTGGGTGAAGATTATAGCATCCATTATGCTCCACTGATTTTGAAGAAA	5	0.125	No Hit
TTCTGCTTCTGCCAAAAACAAGAAATCCGCAGACCCAGTGGATTAGACCT	5	0.125	No Hit
GAAAAACAATCCATTTAATCACACTTGTTTTTGTGGTGGCCTTCAGAAGA	5	0.125	No Hit
TTCTGTTCTCAGCCCTTTCCAAAAAAGCAGTTTCCAGATGTGTTATTTTG	5	0.125	No Hit
CCATCTGCCACCTGCTCACCAGGTCTGCTTTCTGAGATGCTTCCAGATGA	5	0.125	No Hit
TGTTGTGGTAGAGATATTCAGGTTGCTAGTTCTGGGTGTACAGTGGACAG	5	0.125	No Hit
ATTAAACCCATTGAACCCATTGGTTCATCAAGATAGCCTTAAATGAAGTC	5	0.125	No Hit
TGAGGCTCTATGGCAGTCCCGTTTGCTAGTAGGATTGCAAAGAGCTGGAT	5	0.125	No Hit
CCGTTTGCTAGTAGGATTGCAAAGAGCTGGATAGTTGAGTTCTTCCATTG	5	0.125	No Hit
GATCACCTCTTCATCTGCTGTGATAGATTCCAGCAACCTTGCTTCTCCCC	5	0.125	No Hit
CTCCACCACTGAGCTGAATTCCCAACCTCCAAATTACCATTTTCTATTGT	5	0.125	No Hit
CTTGGATCTTATTACCTGGGAAAAGACCCAGCCTCTTCTTCTGGGACTTT	5	0.125	No Hit
TTGGATTTGACAGAAAGCTTAATTCAATATTAGCATAGTAATTAAAAATA	5	0.125	No Hit
CCAAGGAATTCCAGTGATGCTTGTGGTTGAGTGGCCCTCTGAGTAGCCAA	5	0.125	No Hit
TATAAAGACATCACTTATATTGGACTGGGGTACCCTAATGACTCCATTTC	5	0.125	No Hit
CCGTAGTCAGTCTCAATTAAACCCATTGAACCCATTGGTTCATCAAGATA	5	0.125	No Hit
AGATCAAAATGAGCAGAGATGCCAAGTTGTGCTCAACATGTGGGTAGCTT	5	0.125	No Hit
CCAAGTTTTAAAAATTCATAGAAGCAAAGAACACCATCAAGGCAAAAGCT	5	0.125	No Hit
AGGGAATAGTACATGCTAGTGTTGACCTAAGAACTGGACACTTTCAGCTA	5	0.125	No Hit
GGTCGACTCAGGCAGGACCTGCTAGCCCGGCGCTCCCGCCCCTTGCTGCA	5	0.125	No Hit
AAGAAGAATAATGGAGTCAAAACCTGCAAGGTACTCAGCCAGTGTCTGGT	5	0.125	No Hit
TCTTAGCTCTCCCTGCTGATTTGGATTTGACAGAAAGCTTAATTCAATAT	5	0.125	No Hit
CCAACCTTCACCAATGATCCTGTAAGAGACCCTTCACTGGTGCTCCCGTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0125	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.025	0.0	0.0	0.0	0.0
82-83	0.025	0.0	0.0	0.0	0.0
84-85	0.025	0.0	0.0	0.0	0.0
86-87	0.025	0.0	0.0	0.0	0.0
88-89	0.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101056 spots for SRR25925850.sra
Written 9101056 spots for SRR25925850.sra
Read 9101063 spots for SRR25925850.sra
Written 9101063 spots for SRR25925850.sra
SRR ids: ['SRR25925850.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_e660rpsm
SRR25925850.sra spots: 72808455
blocks: [[1, 9101056], [9101057, 18202112], [18202113, 27303168], [27303169, 36404224], [36404225, 45505280], [45505281, 54606336], [54606337, 63707392], [63707393, 72808455]]
SRR25925850 file size 17611608
SRR25925850 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25925850 SRR25925850_1.fastq SRR25925850_2.fastq
Input file:	SRR25925850_1.fastq
Paired file:	SRR25925850_2.fastq
trimmed:	SRR25925850-trimmed-pair1.fastq, SRR25925850-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue May  5 18:21:59 2026 >> started

Tue May  5 18:23:16 2026 >> done (76.872s)
72808455 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
72808455 (100.00%) read pairs available; of these:
  249979 ( 0.34%) trimmed read pairs available after processing
72558476 (99.66%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       3	  0.00%
 66	      17	  0.00%
 67	     213	  0.00%
 68	    1449	  0.00%
 69	    1719	  0.00%
 70	    2101	  0.00%
 71	    2234	  0.00%
 72	    2392	  0.00%
 73	    2663	  0.00%
 74	    2761	  0.00%
 75	    2967	  0.00%
 76	    3259	  0.00%
 77	    3525	  0.00%
 78	    3774	  0.01%
 79	    4086	  0.01%
 80	    4408	  0.01%
 81	    4853	  0.01%
 82	    5206	  0.01%
 83	    5414	  0.01%
 84	    5949	  0.01%
 85	    6281	  0.01%
 86	    6772	  0.01%
 87	    7435	  0.01%
 88	    7765	  0.01%
 89	    8666	  0.01%
 90	    9023	  0.01%
 91	    9916	  0.01%
 92	   10746	  0.01%
 93	   11409	  0.02%
 94	   12009	  0.02%
 95	   12949	  0.02%
 96	   13586	  0.02%
 97	   14766	  0.02%
 98	   16059	  0.02%
 99	   21932	  0.03%
100	   21672	  0.03%
101	72558476	 99.66%
72808455 reads passed initial QC


criterion=sequence-density
sequence-density=0.27
sequence-density-rank=1
fanout-score=2.49
fanout-score-rank=33
prefix-density=0.30
prefix-fanout=2.3
sequence=AAAAGCAGGAGGAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=30
fanout-score=99.68
fanout-score-rank=1
prefix-density=0.18
prefix-fanout=10.4
sequence=TTTCTTTTTAATCACATAAAGGAATATATTTGTAATTCACAATGCGAGTTGGGTTCCTGAACACTTTAGGTATGAAAGATTGTTCATCAACTTGCTATCAAATTAAAATTTTCAAAACCAGTATGGCTTTGAAAGTTATTGTATAATAATGGGGGTGTGTGTTAATTTCTTAAATGTTGATTCAATACACATAAATGCAGGCATTTTCCAGAACAACAAGATAGGTAACAGCATTAAAGCATAATTCCTTACGAGTTAATGAGGAAAGGGGTAACAGGATTCAAGAGTCTTTTCCAGGCATTTTAAATTTTCTGTAAAACTCTT


criterion=sequence-density
sequence-density=0.26
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=38
prefix-density=0.26
prefix-fanout=2.0
sequence=GGCTCCGGCTCTGGCTC


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=25
fanout-score=97.15
fanout-score-rank=1
prefix-density=0.49
prefix-fanout=15.3
sequence=TTTTGTTTTGTTTT
SRR25925850 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 05 18:23:39
                             Started mapping on |	May 05 18:23:39
                                    Finished on |	May 05 18:27:38
       Mapping speed, Million of reads per hour |	1096.70

                          Number of input reads |	72808455
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	68412902
                        Uniquely mapped reads % |	93.96%
                          Average mapped length |	201.34
                       Number of splices: Total |	21300723
            Number of splices: Annotated (sjdb) |	21011342
                       Number of splices: GT/AG |	21069202
                       Number of splices: GC/AG |	164107
                       Number of splices: AT/AC |	23107
               Number of splices: Non-canonical |	44307
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.68
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4388897
             % of reads mapped to multiple loci |	6.03%
        Number of reads mapped to too many loci |	3867
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.00%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6656	6656	6656
N_multimapping	4388897	4388897	4388897
N_noFeature	28942667	66397101	29470989
N_ambiguous	2003351	61374	545846
UnstrandedReadsAssigned:37466884 PositiveStrandReadsAssigned:1954427 NegativeStrandReadsAssigned:38396067
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR25925850 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR25925850-trimmed-pair1.fastq
                             SRR25925850-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 72,808,455 reads, 38,856,231 reads pseudoaligned
[quant] estimated average fragment length: 246.793
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,306 rounds

 109283 SRR25925850.ke.tsv
  52636 SRR25925850.se.tsv
 161919 total
==> SRR25925850.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR25925850.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	9
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25925850 completed mapping pipeline successfully
