Starting /dee2/code/volunteer_pipeline.sh SRR25943868
    current disk space = 1259961472
    free memory = 192737032 
SRR25943868 SRAfilesize
d697af0e610db3ee0154c6e35cc2f0d3  SRR25943868.sra
SRR25943868.sra file validated
SRR25943868 is single end
SRR25943868 is conventional basespace
SRR25943868 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.402	32.0	32.0	32.0	32.0	32.0
2	31.42125	32.0	32.0	32.0	32.0	32.0
3	31.558	32.0	32.0	32.0	32.0	32.0
4	31.6515	32.0	32.0	32.0	32.0	32.0
5	31.6225	32.0	32.0	32.0	32.0	32.0
6	35.21525	36.0	36.0	36.0	36.0	36.0
7	35.2725	36.0	36.0	36.0	36.0	36.0
8	34.9555	36.0	36.0	36.0	36.0	36.0
9	35.0305	36.0	36.0	36.0	36.0	36.0
10	35.10675	36.0	36.0	36.0	36.0	36.0
11	34.96625	36.0	36.0	36.0	36.0	36.0
12	35.208	36.0	36.0	36.0	36.0	36.0
13	35.0515	36.0	36.0	36.0	36.0	36.0
14	35.19075	36.0	36.0	36.0	36.0	36.0
15	34.9495	36.0	36.0	36.0	36.0	36.0
16	35.0655	36.0	36.0	36.0	36.0	36.0
17	34.9555	36.0	36.0	36.0	36.0	36.0
18	35.01125	36.0	36.0	36.0	36.0	36.0
19	35.08825	36.0	36.0	36.0	36.0	36.0
20	34.97475	36.0	36.0	36.0	36.0	36.0
21	35.1705	36.0	36.0	36.0	36.0	36.0
22	35.132	36.0	36.0	36.0	36.0	36.0
23	35.06625	36.0	36.0	36.0	36.0	36.0
24	35.10475	36.0	36.0	36.0	36.0	36.0
25	34.62225	36.0	36.0	36.0	32.0	36.0
26	34.91725	36.0	36.0	36.0	36.0	36.0
27	35.06175	36.0	36.0	36.0	36.0	36.0
28	34.733	36.0	36.0	36.0	32.0	36.0
29	35.044	36.0	36.0	36.0	36.0	36.0
30	34.90775	36.0	36.0	36.0	32.0	36.0
31	35.03225	36.0	36.0	36.0	36.0	36.0
32	34.901	36.0	36.0	36.0	32.0	36.0
33	34.87025	36.0	36.0	36.0	32.0	36.0
34	35.05125	36.0	36.0	36.0	36.0	36.0
35	35.08875	36.0	36.0	36.0	36.0	36.0
36	35.07875	36.0	36.0	36.0	36.0	36.0
37	34.97975	36.0	36.0	36.0	36.0	36.0
38	34.979	36.0	36.0	36.0	36.0	36.0
39	34.933	36.0	36.0	36.0	32.0	36.0
40	34.62775	36.0	36.0	36.0	32.0	36.0
41	34.88675	36.0	36.0	36.0	32.0	36.0
42	34.90425	36.0	36.0	36.0	32.0	36.0
43	34.65325	36.0	36.0	36.0	32.0	36.0
44	34.715	36.0	36.0	36.0	32.0	36.0
45	34.63925	36.0	36.0	36.0	32.0	36.0
46	34.59	36.0	36.0	36.0	32.0	36.0
47	34.676	36.0	36.0	36.0	32.0	36.0
48	34.45725	36.0	36.0	36.0	32.0	36.0
49	34.6905	36.0	36.0	36.0	32.0	36.0
50	34.40025	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	2.0
25	9.0
26	16.0
27	21.0
28	23.0
29	60.0
30	100.0
31	120.0
32	173.0
33	293.0
34	553.0
35	2628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4	14.549999999999999	25.3	27.750000000000004
2	29.975	19.525000000000002	26.674999999999997	23.825
3	39.775	20.674999999999997	20.424999999999997	19.125
4	32.65	21.9	22.15	23.3
5	14.825	26.325	32.625	26.224999999999998
6	32.208052013003254	24.756189047261813	26.25656414103526	16.779194798699677
7	35.699999999999996	16.225	33.550000000000004	14.524999999999999
8	24.15	15.8	19.975	40.075
9	20.825	17.2	33.425	28.549999999999997
10	25.6	14.374999999999998	30.25	29.775000000000002
11	22.275	40.25	14.45	23.025000000000002
12	41.775	18.925	18.0	21.3
13	38.65966491622906	30.48262065516379	15.57889472368092	15.27881970492623
14	18.425	18.125	28.525	34.925
15	13.675	35.675000000000004	19.775000000000002	30.875000000000004
16	13.725000000000001	36.55	25.775	23.95
17	18.45	24.375	25.025	32.15
18	17.95	15.7	42.525	23.825
19	20.375	24.65	30.4	24.575
20	32.6	15.725	24.675	27.0
21	14.725	17.825	39.900000000000006	27.55
22	20.45	18.375	32.175	28.999999999999996
23	35.125	17.45	23.0	24.425
24	28.575	19.375	25.2	26.85
25	0.95	4.65	86.0	8.4
26	11.75	27.675	19.075	41.5
27	27.55	26.724999999999998	23.275000000000002	22.45
28	13.850000000000001	24.025	32.925	29.2
29	34.25	24.3	12.925	28.525
30	26.674999999999997	16.875	29.299999999999997	27.150000000000002
31	37.2	20.7	15.45	26.650000000000002
32	29.525000000000002	19.725	19.025	31.724999999999998
33	29.275000000000002	22.1	17.575	31.05
34	22.05	20.474999999999998	32.975	24.5
35	27.025	18.775	30.425	23.775
36	31.825	10.8	34.375	23.0
37	23.474999999999998	34.699999999999996	24.725	17.1
38	34.0	11.799999999999999	19.400000000000002	34.8
39	32.9	26.85	17.775	22.475
40	20.775	26.924999999999997	19.025	33.275
41	18.325	29.425	22.900000000000002	29.349999999999998
42	33.158289572393095	16.52913228307077	28.657164291072768	21.655413853463365
43	26.625	21.7	20.75	30.925000000000004
44	27.125	15.55	26.875	30.45
45	11.927981995498875	28.657164291072768	19.30482620655164	40.11002750687672
46	18.425	30.825000000000003	32.0	18.75
47	27.725	15.024999999999999	15.275	41.975
48	18.154538634658664	23.705926481620406	32.45811452863216	25.681420355088775
49	34.08352088022005	22.73068267066767	21.930482620655166	21.255313828457115
50	14.075	25.5	22.475	37.95
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	7.0
30	14.0
31	7.5
32	1.0
33	1.0
34	1.0
35	5.0
36	9.0
37	15.5
38	22.0
39	53.0
40	84.0
41	176.0
42	268.0
43	300.0
44	332.0
45	294.0
46	256.0
47	211.5
48	167.0
49	439.0
50	711.0
51	601.0
52	491.0
53	354.5
54	218.0
55	339.0
56	460.0
57	353.5
58	247.0
59	353.5
60	460.0
61	306.5
62	153.0
63	103.5
64	54.0
65	39.5
66	25.0
67	20.0
68	15.0
69	11.5
70	8.0
71	5.5
72	3.0
73	1.5
74	0.0
75	0.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.025
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.025
43	0.0
44	0.0
45	0.025
46	0.0
47	0.0
48	0.025
49	0.025
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.575000000000003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9213863060017	20.974999999999998
2	12.679628064243447	7.5
3	4.142011834319527	3.675
4	2.4513947590870666	2.9000000000000004
5	2.197802197802198	3.25
6	1.183431952662722	2.1
7	1.183431952662722	2.45
8	0.760777683854607	1.7999999999999998
9	0.760777683854607	2.025
>10	2.789518174133559	16.875
>50	0.422654268808115	7.9750000000000005
>100	0.5071851225697379	28.475
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	364	9.1	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	231	5.775	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	222	5.55	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	110	2.75	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	109	2.725	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	103	2.5749999999999997	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	88	2.1999999999999997	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	66	1.6500000000000001	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	57	1.425	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	54	1.35	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	54	1.35	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	40	1.0	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	39	0.975	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	38	0.95	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	35	0.8750000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	32	0.8	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	31	0.775	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	30	0.75	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	30	0.75	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	29	0.7250000000000001	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	29	0.7250000000000001	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	27	0.675	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	23	0.575	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	20	0.5	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	20	0.5	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	19	0.475	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	18	0.44999999999999996	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	16	0.4	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	15	0.375	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	15	0.375	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	15	0.375	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	14	0.35000000000000003	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	14	0.35000000000000003	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	13	0.325	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	13	0.325	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	12	0.3	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	12	0.3	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	12	0.3	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	12	0.3	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	11	0.27499999999999997	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	11	0.27499999999999997	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	10	0.25	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	10	0.25	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	10	0.25	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	9	0.22499999999999998	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	9	0.22499999999999998	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	9	0.22499999999999998	No Hit
TCACTTCGCACTTCTCAAACAATGTAAAATTGGGCTCGAACCCGCCACCA	9	0.22499999999999998	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	9	0.22499999999999998	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	9	0.22499999999999998	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	9	0.22499999999999998	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	9	0.22499999999999998	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	8	0.2	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	8	0.2	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	8	0.2	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	8	0.2	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	8	0.2	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	8	0.2	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	8	0.2	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	8	0.2	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	8	0.2	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	7	0.17500000000000002	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	7	0.17500000000000002	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	7	0.17500000000000002	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	7	0.17500000000000002	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	7	0.17500000000000002	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	7	0.17500000000000002	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	7	0.17500000000000002	No Hit
GGAGAGCATATCTCAGTGTTGTGCTCATTGACTCTGTCCCACCAATAAAA	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	7	0.17500000000000002	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	7	0.17500000000000002	No Hit
TGATGTGCTTCTGGTATTGAGTGGTCTTGTAGTCACAGTTGGGATACTTG	7	0.17500000000000002	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	7	0.17500000000000002	No Hit
CTCCTTCTTCGAGACTTTCACAATTTTTATGCTGCTGCCCGAAGTCTTCT	7	0.17500000000000002	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	6	0.15	No Hit
TGTAGATGCGGAAGATGTCATCTTTCACATAGGACATAGCCATTGTCAGC	6	0.15	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	6	0.15	No Hit
TTCTTAGTGGGCAGACCCAGCTGGCCTTCAGACTGCTTGCTGTTCCTGGC	6	0.15	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	6	0.15	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	6	0.15	No Hit
AGCTCGTCCTCTGTGGTCTTCTGGTAGACTGGGTTGTCAAAGTTTATGCT	6	0.15	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	6	0.15	No Hit
TCTTTACCACTGATCCACAATCAACCCGTTTTCTACCAACAGTATAGGAG	6	0.15	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	6	0.15	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	6	0.15	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	6	0.15	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	6	0.15	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	6	0.15	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	5	0.125	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	5	0.125	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	5	0.125	No Hit
TAAGTACGGTCTTCATAGTGAACCTCCTTGAACAGAAGTTGAAGAGGTCT	5	0.125	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	5	0.125	No Hit
ACTTCTGGCACTAGGACCATAATGTCATGTCCTCGTTCACTGAGATGTTC	5	0.125	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTTCAGTGAC	5	0.125	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	5	0.125	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATGGACTTCTCAGTGAC	5	0.125	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	5	0.125	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	5	0.125	No Hit
CTATGCTTTAGTGGGATTTCCTGCAGGTTTGGGATCGTAACCGTACAAGA	5	0.125	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	5	0.125	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	5	0.125	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	5	0.125	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	5	0.125	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	5	0.125	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCATCTC	5	0.125	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	5	0.125	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	5	0.125	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	5	0.125	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	5	0.125	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	40	5.315087E-9	44.000004	6
GAGGTAT	40	5.315087E-9	44.000004	1
GGAGAAG	20	6.952051E-4	44.000004	11
GAGAAGT	20	6.952051E-4	44.000004	12
CCGGAGA	20	6.952051E-4	44.000004	9
TGGCAAC	20	6.952051E-4	44.000004	25
CGGAGAA	20	6.952051E-4	44.000004	10
ACATGTG	40	5.315087E-9	44.000004	16
CAGCAGG	20	6.952051E-4	44.000004	18
CAGCAGC	20	6.952051E-4	44.000004	15
AGAAGTT	20	6.952051E-4	44.000004	13
AGGACTT	20	6.952051E-4	44.000004	1
ACTTGGT	20	6.952051E-4	44.000004	4
AACGAGG	20	6.952051E-4	44.000004	29
TCCTAGG	40	5.315087E-9	44.000004	7
GTACTTG	20	6.952051E-4	44.000004	2
GGACTTG	20	6.952051E-4	44.000004	2
ACTTGCC	20	6.952051E-4	44.000004	4
GGACTTC	40	5.315087E-9	44.000004	37
CCAGCAA	20	6.952051E-4	44.000004	26
>>END_MODULE
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520476 spots for SRR25943868.sra
Written 520476 spots for SRR25943868.sra
Read 520477 spots for SRR25943868.sra
Written 520477 spots for SRR25943868.sra
SRR ids: ['SRR25943868.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tkapfiuw
SRR25943868.sra spots: 4163809
blocks: [[1, 520476], [520477, 1040952], [1040953, 1561428], [1561429, 2081904], [2081905, 2602380], [2602381, 3122856], [3122857, 3643332], [3643333, 4163809]]
SRR25943868 file size 719432
SRR25943868 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943868 SRR25943868_1.fastq
Input file:	SRR25943868_1.fastq
trimmed:	SRR25943868-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:35:58 2026 >> started

Tue May  5 05:35:59 2026 >> done (1.209s)
4163809 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
4163809 (100.00%) reads available; of these:
     86 ( 0.00%) trimmed reads available after processing
4163723 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      2	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      2	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	     11	  0.00%
 47	      5	  0.00%
 48	     29	  0.00%
 49	     37	  0.00%
 50	4163723	100.00%
4163809 reads passed initial QC


criterion=sequence-density
sequence-density=9.53
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=10
prefix-density=0.04
prefix-fanout=1.0
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTG


criterion=fanout-score
sequence-density=0.38
sequence-density-rank=40
fanout-score=32.75
fanout-score-rank=1
prefix-density=12.28
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:36:05
                             Started mapping on |	May 05 05:36:05
                                    Finished on |	May 05 05:36:13
       Mapping speed, Million of reads per hour |	1873.71

                          Number of input reads |	4163809
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2832894
                        Uniquely mapped reads % |	68.04%
                          Average mapped length |	49.57
                       Number of splices: Total |	1892
            Number of splices: Annotated (sjdb) |	786
                       Number of splices: GT/AG |	1745
                       Number of splices: GC/AG |	31
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	116
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.07%
                        Deletion average length |	1.07
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	511682
             % of reads mapped to multiple loci |	12.29%
        Number of reads mapped to too many loci |	725860
             % of reads mapped to too many loci |	17.43%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.24%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	819233	819233	819233
N_multimapping	511682	511682	511682
N_noFeature	9865	2762823	9877
N_ambiguous	95800	48	26363
UnstrandedReadsAssigned:2727229 PositiveStrandReadsAssigned:70023 NegativeStrandReadsAssigned:2796654
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943868 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943868-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,163,809 reads, 3,910,110 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,195 rounds

 109283 SRR25943868.ke.tsv
  52636 SRR25943868.se.tsv
 161919 total
==> SRR25943868.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943868.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943868 completed mapping pipeline successfully
