Starting /dee2/code/volunteer_pipeline.sh SRR25943827
    current disk space = 1266362332
    free memory = 193872216 
SRR25943827 SRAfilesize
b967d906e6fe186a7c771f8600ba4f34  SRR25943827.sra
SRR25943827.sra file validated
SRR25943827 is single end
SRR25943827 is conventional basespace
SRR25943827 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39525	32.0	32.0	32.0	32.0	32.0
2	31.59525	32.0	32.0	32.0	32.0	32.0
3	31.52825	32.0	32.0	32.0	32.0	32.0
4	31.604	32.0	32.0	32.0	32.0	32.0
5	31.635	32.0	32.0	32.0	32.0	32.0
6	34.99325	36.0	36.0	36.0	36.0	36.0
7	35.143	36.0	36.0	36.0	36.0	36.0
8	34.939	36.0	36.0	36.0	32.0	36.0
9	35.01925	36.0	36.0	36.0	32.0	36.0
10	34.97675	36.0	36.0	36.0	32.0	36.0
11	35.06725	36.0	36.0	36.0	36.0	36.0
12	34.997	36.0	36.0	36.0	36.0	36.0
13	35.0775	36.0	36.0	36.0	36.0	36.0
14	35.056	36.0	36.0	36.0	36.0	36.0
15	35.08	36.0	36.0	36.0	36.0	36.0
16	34.9255	36.0	36.0	36.0	36.0	36.0
17	35.03975	36.0	36.0	36.0	36.0	36.0
18	35.003	36.0	36.0	36.0	36.0	36.0
19	35.0125	36.0	36.0	36.0	36.0	36.0
20	35.11725	36.0	36.0	36.0	36.0	36.0
21	35.0995	36.0	36.0	36.0	36.0	36.0
22	35.139	36.0	36.0	36.0	36.0	36.0
23	35.105	36.0	36.0	36.0	36.0	36.0
24	35.068	36.0	36.0	36.0	36.0	36.0
25	35.224	36.0	36.0	36.0	36.0	36.0
26	34.79675	36.0	36.0	36.0	32.0	36.0
27	34.93925	36.0	36.0	36.0	36.0	36.0
28	34.737	36.0	36.0	36.0	32.0	36.0
29	34.96375	36.0	36.0	36.0	36.0	36.0
30	34.7225	36.0	36.0	36.0	32.0	36.0
31	34.99625	36.0	36.0	36.0	36.0	36.0
32	34.82825	36.0	36.0	36.0	32.0	36.0
33	34.897	36.0	36.0	36.0	32.0	36.0
34	34.8845	36.0	36.0	36.0	32.0	36.0
35	34.8575	36.0	36.0	36.0	32.0	36.0
36	34.873	36.0	36.0	36.0	32.0	36.0
37	34.82575	36.0	36.0	36.0	32.0	36.0
38	34.70475	36.0	36.0	36.0	32.0	36.0
39	34.7885	36.0	36.0	36.0	32.0	36.0
40	34.31475	36.0	36.0	36.0	32.0	36.0
41	34.652	36.0	36.0	36.0	32.0	36.0
42	34.7055	36.0	36.0	36.0	32.0	36.0
43	34.62525	36.0	36.0	36.0	32.0	36.0
44	34.5895	36.0	36.0	36.0	32.0	36.0
45	34.4175	36.0	36.0	36.0	32.0	36.0
46	34.32625	36.0	36.0	36.0	32.0	36.0
47	34.3925	36.0	36.0	36.0	32.0	36.0
48	34.24225	36.0	36.0	36.0	32.0	36.0
49	34.44575	36.0	36.0	36.0	32.0	36.0
50	34.16175	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	-0.059586681974739264
11101	2	0.17347876004592422
11101	3	0.09334098737083707
11101	4	-0.12583237657864288
11101	5	-0.15671641791044877
11101	6	0.28220436280138017
11101	7	0.16727898966705368
11101	8	-0.33811710677382223
11101	9	0.21228473019517935
11101	10	-0.15809414466130534
11101	11	0.300803673938006
11101	12	-0.14684270952927392
11101	13	-0.08484500574052589
11101	14	-0.14270952927669356
11101	15	0.02273249138920619
11101	16	-0.4398392652123988
11101	17	0.08025258323765883
11101	18	-0.19402985074626855
11101	19	-0.34385763490240606
11101	20	-0.18266360505166546
11101	21	-0.35338691159586944
11101	22	-0.10688863375430913
11101	23	0.30734787600459157
11101	24	-0.06417910447760988
11101	25	0.025373134328361857
11101	26	0.0251435132032114
11101	27	0.06693455797933012
11101	28	-0.29070034443168424
11101	29	0.11756601607348216
11101	30	-0.17072330654420398
11101	31	0.28025258323765456
11101	32	-0.2115958668197493
11101	33	-0.0035591274397219763
11101	34	-0.1276693455797897
11101	35	-0.25017221584385396
11101	36	-0.12215843857634212
11101	37	-0.12893226176808525
11101	38	-0.45729047072330786
11101	39	-0.31825487944890796
11101	40	0.025717566016076887
11101	41	0.020780711825487685
11101	42	-0.4649827784156102
11101	43	-0.325028702640644
11101	44	-0.25706084959816167
11101	45	-0.37256027554534654
11101	46	0.03168771526980407
11101	47	-0.1444316877152758
11101	48	-0.009988518943742974
11101	49	-0.40757749712973634
11101	50	-0.49127439724454547
11102	1	0.05958668197474282
11102	2	-0.17347876004592422
11102	3	-0.09334098737084062
11102	4	0.12583237657864643
11102	5	0.15671641791044522
11102	6	-0.28220436280138017
11102	7	-0.16727898966704657
11102	8	0.33811710677382933
11102	9	-0.21228473019517935
11102	10	0.15809414466131244
11102	11	-0.3008036739379989
11102	12	0.14684270952928102
11102	13	0.084845005740533
11102	14	0.14270952927669356
11102	15	-0.02273249138920619
11102	16	0.4398392652123988
11102	17	-0.08025258323765883
11102	18	0.19402985074626855
11102	19	0.34385763490241317
11102	20	0.18266360505166546
11102	21	0.35338691159586233
11102	22	0.10688863375430202
11102	23	-0.30734787600459157
11102	24	0.06417910447761699
11102	25	-0.02537313432835475
11102	26	-0.0251435132032114
11102	27	-0.06693455797933723
11102	28	0.29070034443169135
11102	29	-0.11756601607348216
11102	30	0.17072330654420398
11102	31	-0.28025258323766167
11102	32	0.21159586681974218
11102	33	0.0035591274397290817
11102	34	0.1276693455797968
11102	35	0.25017221584386107
11102	36	0.12215843857634923
11102	37	0.12893226176807815
11102	38	0.45729047072330786
11102	39	0.31825487944890796
11102	40	-0.02571756601606978
11102	41	-0.020780711825487685
11102	42	0.4649827784156173
11102	43	0.325028702640644
11102	44	0.25706084959816167
11102	45	0.37256027554535365
11102	46	-0.03168771526980407
11102	47	0.1444316877152687
11102	48	0.009988518943742974
11102	49	0.40757749712973634
11102	50	0.49127439724454547
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	2.0
25	7.0
26	14.0
27	27.0
28	40.0
29	62.0
30	94.0
31	137.0
32	197.0
33	291.0
34	637.0
35	2490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.500000000000004	20.375	25.924999999999997	26.200000000000003
2	22.0	18.3	21.55	38.15
3	20.525	24.25	23.0	32.225
4	20.150000000000002	24.625	28.4	26.825
5	17.325	25.95	23.825	32.9
6	28.86107634543179	30.062578222778473	21.451814768460576	19.62453066332916
7	21.224999999999998	24.375	32.675	21.725
8	21.875	20.4	20.45	37.275000000000006
9	25.224999999999998	16.55	24.775	33.45
10	18.975	21.95	31.65	27.425
11	25.45	15.7	22.0	36.85
12	25.124999999999996	19.225	26.325	29.325000000000003
13	24.15603900975244	24.18104526131533	31.332833208302073	20.330082520630157
14	22.2	17.325	27.725	32.75
15	20.424999999999997	25.7	22.6	31.275
16	15.15	23.724999999999998	29.425	31.7
17	21.625	19.725	26.200000000000003	32.45
18	23.275000000000002	15.9	39.2	21.625
19	25.124999999999996	22.425	30.475	21.975
20	26.700000000000003	16.85	27.3	29.15
21	20.65	18.05	23.325000000000003	37.974999999999994
22	15.950000000000001	23.575	35.175	25.3
23	28.225	13.0	26.450000000000003	32.324999999999996
24	30.675	12.275	20.599999999999998	36.449999999999996
25	1.975	3.2750000000000004	79.3	15.45
26	19.875	19.375	26.0	34.75
27	30.5	18.4	21.9	29.2
28	13.750000000000002	24.975	28.925	32.35
29	21.6	28.925	22.125	27.35
30	20.575	20.424999999999997	27.700000000000003	31.3
31	19.775000000000002	27.6	27.375	25.25
32	22.6	18.7	21.425	37.275000000000006
33	32.574999999999996	18.675	23.1	25.650000000000002
34	28.925	15.25	34.075	21.75
35	24.95	18.175	22.675	34.2
36	21.525	15.575	22.175	40.725
37	19.15	27.025	25.724999999999998	28.1
38	27.675	14.099999999999998	23.025000000000002	35.199999999999996
39	29.525000000000002	18.675	23.25	28.549999999999997
40	21.325	23.974999999999998	30.075000000000003	24.625
41	20.45	24.775	20.849999999999998	33.925
42	27.56378189094547	21.860930465232617	18.934467233616807	31.6408204102051
43	26.950000000000003	21.725	29.95	21.375
44	26.125	19.675	20.8	33.4
45	18.98449224612306	18.859429714857427	23.88694347173587	38.26913456728364
46	22.3	21.05	28.775000000000002	27.875
47	26.875	17.375	18.875	36.875
48	22.441831373530146	27.295471603702776	17.838378784088064	32.424318238679014
49	24.706176544136035	23.705926481620406	29.68242060515129	21.905476369092273
50	21.575	14.149999999999999	15.8	48.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	1.0
34	2.0
35	2.5
36	3.0
37	11.5
38	20.0
39	33.0
40	46.0
41	116.0
42	186.0
43	143.5
44	101.0
45	161.0
46	221.0
47	234.0
48	247.0
49	437.0
50	627.0
51	675.0
52	723.0
53	557.5
54	392.0
55	444.0
56	496.0
57	452.0
58	408.0
59	280.0
60	152.0
61	120.5
62	89.0
63	89.5
64	90.0
65	86.5
66	83.0
67	65.5
68	48.0
69	40.5
70	33.0
71	31.0
72	29.0
73	15.5
74	2.0
75	1.0
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.125
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.025
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.05
43	0.0
44	0.0
45	0.05
46	0.0
47	0.0
48	0.075
49	0.025
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03225806451613	28.299999999999997
2	9.483870967741934	7.35
3	4.645161290322581	5.4
4	3.225806451612903	5.0
5	2.258064516129032	4.375
6	1.2258064516129032	2.85
7	0.8387096774193548	2.275
8	0.8387096774193548	2.6
9	0.4516129032258065	1.575
>10	3.5483870967741935	22.425
>50	0.3225806451612903	9.9
>100	0.12903225806451613	7.95
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	208	5.2	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	110	2.75	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	90	2.25	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	88	2.1999999999999997	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	85	2.125	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	77	1.925	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	56	1.4000000000000001	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	32	0.8	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	30	0.75	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	29	0.7250000000000001	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	28	0.7000000000000001	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	27	0.675	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	26	0.65	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	26	0.65	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	24	0.6	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	23	0.575	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	23	0.575	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	21	0.525	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	21	0.525	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	20	0.5	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	19	0.475	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	18	0.44999999999999996	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	17	0.42500000000000004	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	17	0.42500000000000004	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	17	0.42500000000000004	No Hit
GTCCACCAGAGCTTCCTTGACTGTCTCCTGTCCGCACAGCACCACAATTC	17	0.42500000000000004	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	16	0.4	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	16	0.4	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	16	0.4	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	16	0.4	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	15	0.375	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	15	0.375	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	15	0.375	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	15	0.375	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	15	0.375	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	15	0.375	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	15	0.375	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	14	0.35000000000000003	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	14	0.35000000000000003	No Hit
CCAGCTGCCCTTGAGTGATGTACTTTTTCCACCAGAGGTAGGGACGCATG	14	0.35000000000000003	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	14	0.35000000000000003	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	13	0.325	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	13	0.325	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	13	0.325	No Hit
TTCTGCCATCAGCTGTCGTTTCATCAAACTTCTCTCCCAGGTTACAAGAG	12	0.3	No Hit
AGAAAGGTCAGAGAAGGGATGATATTCACTAGGTCAGCAGCAAATCCTTC	12	0.3	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	12	0.3	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	12	0.3	No Hit
TGGTCAGCTCGACTGAAAGCCGCTTCTTGTAATCCTGTGGCTTGTCCTCA	12	0.3	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	12	0.3	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	12	0.3	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	11	0.27499999999999997	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	11	0.27499999999999997	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	11	0.27499999999999997	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	11	0.27499999999999997	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	10	0.25	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	10	0.25	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	10	0.25	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	10	0.25	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	10	0.25	No Hit
AGGATCGAGACGGCTTGTGATTTCTCTCACGAGACAGAGACCTTTCTCTT	10	0.25	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTAT	10	0.25	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	9	0.22499999999999998	No Hit
CATCAGAAGACTCGGACACCAGCTTCCCATCTCGGGTTTCAATCTTCTTC	9	0.22499999999999998	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	9	0.22499999999999998	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	9	0.22499999999999998	No Hit
CCCAAGATCAACATACAGGTGATTTTCTCCATGCTCTTACCAGGCTCTCA	9	0.22499999999999998	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	9	0.22499999999999998	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	9	0.22499999999999998	No Hit
TACTTAGTGACAGCCTTGGTGCCCTCCGACACGGCGTGCTTGGCCAGCTC	8	0.2	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	8	0.2	No Hit
GAAACCTCTCGGACAGCAATCAGCTCCTTTCCATTGTCTACACGTGTGAA	8	0.2	No Hit
GCTTGGTGCAGGCTGACCATGCGGTCGATCAGAGCCCGCCGTGTGGTCTC	8	0.2	No Hit
CTCCTTCTTCGAGACTTTCACAATTTTTATGCTGCTGCCCGAAGTCTTCT	8	0.2	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	8	0.2	No Hit
AGATATGAATGCAGCTCACTCTCCTGGTGGATGCGCCGTTCCTCGATACT	8	0.2	No Hit
TGTGTCCTCTCTGAGCTTCATGTTTTCAGCAAAGACATCCGGTGCCACAC	8	0.2	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	8	0.2	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	8	0.2	No Hit
GAACGCCTTTACCAAACGGAGTGTTTCTTCCACACTGCGGCCCACCGGAA	8	0.2	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	8	0.2	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTC	8	0.2	No Hit
TCATGCTTGATGCCCAGAGACTCCCCCTTGTCTGTGCTCAGCGTGCCTGC	7	0.17500000000000002	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	7	0.17500000000000002	No Hit
GGTCCGTCTTGAAGTCCTGCGCGATCTCGCGCACCAGGCGCTGGAACGGC	7	0.17500000000000002	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	7	0.17500000000000002	No Hit
TGCTTCTGCAGCTCCTCCTTCTGGCACACGCGCTTCACGCGGCAGCTGGC	7	0.17500000000000002	No Hit
CTGCCCGAGATGCTCATGTGCTCCTGCTCACTCAACATCTCTGGCCGTTC	7	0.17500000000000002	No Hit
GAATCTTCTCCTGGGAGCAGCTCCTCCCAGAGAGTCTCTCCGGATGCTCC	7	0.17500000000000002	No Hit
GTGGCTGTGCACTCATAGATGGCTTCGTCTCGCTGCACTCGTAATGGCTG	7	0.17500000000000002	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	7	0.17500000000000002	No Hit
ACAGACCCACAAGGTAGGCCTCGCTCGCCTCCTGCAGAGCCATGACAGCC	7	0.17500000000000002	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	7	0.17500000000000002	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	7	0.17500000000000002	No Hit
GGCACGCATGCTTGTCCCACAGAGAGTCATCTGATCCAGTCATCACGCAG	7	0.17500000000000002	No Hit
GACCATTTCTCCTCGCTGAGCTGGCAGCACGTGGAGGCAATGCTCACCAT	6	0.15	No Hit
CTGACCAGGATGGAGGTGACATTGTAGCTATTGTTCTCTTGTAGCTCATA	6	0.15	No Hit
TTATGAAAGGCAAGAAGTCACTTCCATACATGGAGAAGCCATCAGGGTGC	6	0.15	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	6	0.15	No Hit
TCCAGCCCTCCGTCACTCCCATCTTTCTTCTTCCCATCTCCATCCACCTC	6	0.15	No Hit
GATACACTTTCCCTCATAGGCTAATCCAATGGATCTGCCCAGCAAGCAGG	6	0.15	No Hit
ATTCCAATCCACTGGCCTGTCTGCTTGGCAAACTGGGCAGCCTGGACCTG	6	0.15	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	6	0.15	No Hit
CCTGGCTCAGTGCAGTTCCGCAAATGTAAGTCCTCCTTGGTGAAGCACAG	6	0.15	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	6	0.15	No Hit
ACCTTGCTGCTGCTCCTGCTGCTCTAACTGCAGTGACTGCTGCTGCTTCT	6	0.15	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	6	0.15	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	6	0.15	No Hit
GAGCCTGATCGGAAGTCCATCGTCTCTTCTTCCTGCCTCTTGAGTCCATC	6	0.15	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	6	0.15	No Hit
GTGGAGACTCCTCACAATGCACCGCCTCGAATTGCTCTTGCCCTTCCTAC	6	0.15	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	6	0.15	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	6	0.15	No Hit
ATACTGGCACCGCGGTTGGCAACACCGGCAGAAAAGTCGTTGATGTTGGA	6	0.15	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	5	0.125	No Hit
TTCTACACTGCCTACTGCTTACATTTTCCAAACCCATCAGCATCACTCCT	5	0.125	No Hit
GCAGACGCAACTCTCGGTAGGTCCTTTTGGCGTGAATGATGGACTGAAAC	5	0.125	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	5	0.125	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	5	0.125	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	5	0.125	No Hit
GGCAGTAAAGCAGTCGCTTGGGCGTCCCATGGCGTCTGAAGAAGAAGACA	5	0.125	No Hit
CCTCTGTACAACAGCTCCTCCACCTTGAATCCCAGGTCCAGGGTTTCCAG	5	0.125	No Hit
ACTAGAAGAACCCGTACAAGCATATTTTCCAAGGTGTTCCGGTCCAGGGA	5	0.125	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	5	0.125	No Hit
GACAGATCCGAAGCACCTCTTCTTTTGTAGAAGTTGATAGATGGTTCATT	5	0.125	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	5	0.125	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	5	0.125	No Hit
ATGGCCATGATGGCATTGCGGATGTCCTCCTCATCAGCGTTGGAGCTTAC	5	0.125	No Hit
CGGCGCCAATTCCTTTCACCGAGTTCGCGAGGCAAGACGCGGAGACTCGC	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	5	0.125	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	5	0.125	No Hit
CGCTTCTCCGTGGCCTTACAACACTGCTCAATGCAGCCGGCCATGCCTTT	5	0.125	No Hit
TGGAGATCTGCTGCTCTACCATGGTGATCTCACGGTCCACGCGGTCCATG	5	0.125	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	5	0.125	No Hit
ACATGGCTTGCATAGTACTCGTAGTTCCTCAGTGCAGCCAGGACACCGGC	5	0.125	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	5	0.125	No Hit
TAACACCAACCACAGCAGGAGCAGCTTGAAGAGAGTCACTCGCATGTCTT	5	0.125	No Hit
CTGAGACATCCCAGGAGAAGACACTGCATTCGGAGTCCCTCCATCCTGCT	5	0.125	No Hit
AGATAATCGTAGTCCTGGTCCTGATCCGACTCAGAGGAGTTCAGTGAGCT	5	0.125	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	5	0.125	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	5	0.125	No Hit
AGTAGGTTTCCATCTTGGCAATCTTTTCATCGAAGTACTTCCTGCCATAG	5	0.125	No Hit
GTTGCTAAGCTTCCAACCAAACTCTCCTGCTCCTCAATTTCCTGGTATTC	5	0.125	No Hit
CCTGAGAGACAGAGCAGGCCTGTTCTTCATGTACTTGTTACCCAGCCATA	5	0.125	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
GGCCCTCCTTCGCGGATTGTTCTCTATGCGCTCTACCTTCTTCTCCCACG	5	0.125	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCT	25	3.6958358E-5	44.0	6
GAGGCAC	25	3.6958358E-5	44.0	44
TGGCATG	25	3.6958358E-5	44.0	16
CAAGAGG	25	3.6958358E-5	44.0	41
TCCTTCC	25	3.6958358E-5	44.0	34
GGCATGT	25	3.6958358E-5	44.0	17
CACATGG	25	3.6958358E-5	44.0	12
ACATGGC	25	3.6958358E-5	44.0	13
TTTCTTC	25	3.6958358E-5	44.0	18
TTCGTCC	25	3.6958358E-5	44.0	30
AATTCGT	25	3.6958358E-5	44.0	28
TTTCCAT	25	3.6958358E-5	44.0	12
GTGTAAT	25	3.6958358E-5	44.0	22
TCTGGAA	25	3.6958358E-5	44.0	23
CTCTTTC	25	3.6958358E-5	44.0	9
AGGGCTG	25	3.6958358E-5	44.0	41
AGAGGCA	25	3.6958358E-5	44.0	43
AAGAGGC	25	3.6958358E-5	44.0	42
ATGGCAT	25	3.6958358E-5	44.0	15
CATTTCT	25	3.6958358E-5	44.0	16
>>END_MODULE
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
Read 355852 spots for SRR25943827.sra
Written 355852 spots for SRR25943827.sra
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
Read 355849 spots for SRR25943827.sra
Written 355849 spots for SRR25943827.sra
SRR ids: ['SRR25943827.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bdr2z1cp
SRR25943827.sra spots: 2846795
blocks: [[1, 355849], [355850, 711698], [711699, 1067547], [1067548, 1423396], [1423397, 1779245], [1779246, 2135094], [2135095, 2490943], [2490944, 2846795]]
SRR25943827 file size 491532
SRR25943827 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943827 SRR25943827_1.fastq
Input file:	SRR25943827_1.fastq
trimmed:	SRR25943827-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:40:38 2026 >> started

Tue May  5 05:40:39 2026 >> done (0.770s)
2846795 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2846795 (100.00%) reads available; of these:
     63 ( 0.00%) trimmed reads available after processing
2846732 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	      1	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      2	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	     14	  0.00%
 47	      6	  0.00%
 48	     12	  0.00%
 49	     28	  0.00%
 50	2846732	100.00%
2846795 reads passed initial QC


criterion=sequence-density
sequence-density=5.95
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=21
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCACCCT


criterion=fanout-score
sequence-density=2.71
sequence-density-rank=4
fanout-score=17.42
fanout-score-rank=1
prefix-density=2.92
prefix-fanout=16.2
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTCGTA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:40:45
                             Started mapping on |	May 05 05:40:45
                                    Finished on |	May 05 05:40:49
       Mapping speed, Million of reads per hour |	2562.12

                          Number of input reads |	2846795
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2473769
                        Uniquely mapped reads % |	86.90%
                          Average mapped length |	49.62
                       Number of splices: Total |	1964
            Number of splices: Annotated (sjdb) |	1255
                       Number of splices: GT/AG |	1794
                       Number of splices: GC/AG |	38
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	132
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.09%
                        Deletion average length |	1.13
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	222424
             % of reads mapped to multiple loci |	7.81%
        Number of reads mapped to too many loci |	18645
             % of reads mapped to too many loci |	0.65%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.63%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	150602	150602	150602
N_multimapping	222424	222424	222424
N_noFeature	1453	2406397	1488
N_ambiguous	85097	45	18502
UnstrandedReadsAssigned:2387219 PositiveStrandReadsAssigned:67327 NegativeStrandReadsAssigned:2453779
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943827 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943827-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,846,795 reads, 2,554,549 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,151 rounds

 109283 SRR25943827.ke.tsv
  52636 SRR25943827.se.tsv
 161919 total
==> SRR25943827.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943827.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943827 completed mapping pipeline successfully
