Starting /dee2/code/volunteer_pipeline.sh SRR25943846
    current disk space = 1265660412
    free memory = 195051728 
SRR25943846 SRAfilesize
7d2dbd4056792938773fcb9248fc004d  SRR25943846.sra
SRR25943846.sra file validated
SRR25943846 is single end
SRR25943846 is conventional basespace
SRR25943846 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343	32.0	32.0	32.0	32.0	32.0
2	31.4295	32.0	32.0	32.0	32.0	32.0
3	31.455	32.0	32.0	32.0	32.0	32.0
4	31.6175	32.0	32.0	32.0	32.0	32.0
5	31.492	32.0	32.0	32.0	32.0	32.0
6	35.14075	36.0	36.0	36.0	36.0	36.0
7	34.9985	36.0	36.0	36.0	36.0	36.0
8	35.00225	36.0	36.0	36.0	32.0	36.0
9	35.0965	36.0	36.0	36.0	36.0	36.0
10	34.99825	36.0	36.0	36.0	32.0	36.0
11	35.03625	36.0	36.0	36.0	36.0	36.0
12	35.13425	36.0	36.0	36.0	36.0	36.0
13	35.042	36.0	36.0	36.0	36.0	36.0
14	35.01675	36.0	36.0	36.0	36.0	36.0
15	34.851	36.0	36.0	36.0	32.0	36.0
16	34.99675	36.0	36.0	36.0	36.0	36.0
17	34.85275	36.0	36.0	36.0	32.0	36.0
18	34.8395	36.0	36.0	36.0	36.0	36.0
19	34.95375	36.0	36.0	36.0	36.0	36.0
20	34.931	36.0	36.0	36.0	36.0	36.0
21	35.00575	36.0	36.0	36.0	36.0	36.0
22	34.97825	36.0	36.0	36.0	36.0	36.0
23	35.16775	36.0	36.0	36.0	36.0	36.0
24	35.02875	36.0	36.0	36.0	36.0	36.0
25	35.1015	36.0	36.0	36.0	36.0	36.0
26	34.788	36.0	36.0	36.0	32.0	36.0
27	34.78475	36.0	36.0	36.0	32.0	36.0
28	34.727	36.0	36.0	36.0	32.0	36.0
29	34.97325	36.0	36.0	36.0	36.0	36.0
30	34.82125	36.0	36.0	36.0	32.0	36.0
31	34.8615	36.0	36.0	36.0	32.0	36.0
32	34.86825	36.0	36.0	36.0	32.0	36.0
33	34.86975	36.0	36.0	36.0	32.0	36.0
34	34.9375	36.0	36.0	36.0	36.0	36.0
35	34.94675	36.0	36.0	36.0	36.0	36.0
36	34.85275	36.0	36.0	36.0	36.0	36.0
37	34.884	36.0	36.0	36.0	32.0	36.0
38	34.7425	36.0	36.0	36.0	32.0	36.0
39	34.77	36.0	36.0	36.0	32.0	36.0
40	34.76975	36.0	36.0	36.0	32.0	36.0
41	34.82875	36.0	36.0	36.0	32.0	36.0
42	34.793	36.0	36.0	36.0	32.0	36.0
43	34.506	36.0	36.0	36.0	32.0	36.0
44	34.68225	36.0	36.0	36.0	32.0	36.0
45	34.6385	36.0	36.0	36.0	32.0	36.0
46	34.47675	36.0	36.0	36.0	32.0	36.0
47	34.4865	36.0	36.0	36.0	32.0	36.0
48	34.238	36.0	36.0	36.0	32.0	36.0
49	34.392	36.0	36.0	36.0	32.0	36.0
50	34.16125	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	4.0
25	7.0
26	13.0
27	26.0
28	46.0
29	59.0
30	105.0
31	125.0
32	190.0
33	294.0
34	613.0
35	2516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.925	14.325	22.55	38.2
2	25.674999999999997	14.099999999999998	37.824999999999996	22.400000000000002
3	44.7	19.2	15.225	20.875
4	39.800000000000004	18.6	14.249999999999998	27.35
5	17.0	33.85	23.7	25.45
6	41.44144144144144	19.044044044044046	20.445445445445447	19.06906906906907
7	44.35	14.124999999999998	25.4	16.125
8	17.1	17.375	16.900000000000002	48.625
9	17.95	21.8	36.875	23.375
10	23.95	12.725	24.4	38.925
11	24.925	43.275000000000006	13.975000000000001	17.825
12	41.75	15.825	20.075000000000003	22.35
13	45.475	19.975	14.6	19.950000000000003
14	16.925	17.075000000000003	24.075	41.925000000000004
15	18.099999999999998	26.85	18.475	36.575
16	14.249999999999998	41.15	24.2	20.4
17	17.5	18.8	37.025000000000006	26.674999999999997
18	19.15	12.75	48.8	19.3
19	21.425	21.2	24.224999999999998	33.15
20	24.025	13.3	35.05	27.625
21	13.350000000000001	17.275	46.050000000000004	23.325000000000003
22	13.4	18.6	35.4	32.6
23	28.050000000000004	24.9	21.2	25.85
24	20.375	12.55	35.325	31.75
25	0.75	2.85	82.92500000000001	13.475000000000001
26	11.700000000000001	18.9	19.85	49.55
27	26.8	17.375	33.7	22.125
28	14.95	17.05	27.400000000000002	40.6
29	41.699999999999996	22.975	10.725	24.6
30	34.425	17.974999999999998	21.224999999999998	26.375
31	41.05	22.625	12.1	24.224999999999998
32	20.575	18.525	22.400000000000002	38.5
33	27.025	18.825	16.475	37.675
34	22.35	18.175	40.075	19.400000000000002
35	32.574999999999996	20.75	25.85	20.825
36	43.1	10.375	23.849999999999998	22.675
37	23.974999999999998	39.875	21.0	15.15
38	43.525000000000006	8.774999999999999	17.05	30.65
39	40.150000000000006	21.8	14.475	23.575
40	19.825	21.825	19.400000000000002	38.95
41	15.6	36.125	15.925	32.35
42	45.15	15.125	19.225	20.5
43	22.95	20.674999999999997	17.9	38.475
44	34.65	13.325000000000001	23.925	28.1
45	10.15	39.875	17.349999999999998	32.625
46	15.85	21.325	42.125	20.7
47	25.324999999999996	12.35	14.2	48.125
48	15.425	18.65	42.85	23.075000000000003
49	37.824999999999996	16.725	22.75	22.7
50	14.549999999999999	18.85	29.525000000000002	37.075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	2.0
30	3.0
31	1.5
32	0.0
33	0.0
34	0.0
35	3.0
36	6.0
37	14.0
38	22.0
39	49.0
40	76.0
41	115.5
42	155.0
43	166.5
44	178.0
45	155.5
46	133.0
47	119.5
48	106.0
49	322.5
50	539.0
51	567.0
52	595.0
53	398.0
54	201.0
55	277.0
56	353.0
57	395.5
58	438.0
59	621.0
60	804.0
61	530.5
62	257.0
63	151.5
64	46.0
65	44.5
66	43.0
67	24.5
68	6.0
69	13.5
70	21.0
71	18.0
72	15.0
73	8.0
74	1.0
75	0.5
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.799999999999997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74305555555556	20.95
2	13.020833333333334	7.5
3	3.9930555555555554	3.45
4	1.9965277777777777	2.3
5	1.6493055555555556	2.375
6	1.128472222222222	1.95
7	0.78125	1.575
8	0.607638888888889	1.4000000000000001
9	0.4340277777777778	1.125
>10	2.7777777777777777	18.05
>50	0.3472222222222222	6.15
>100	0.4340277777777778	16.425
>500	0.08680555555555555	16.75
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	670	16.75	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	164	4.1000000000000005	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	146	3.65	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	118	2.9499999999999997	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	115	2.875	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	114	2.85	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	76	1.9	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	60	1.5	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	59	1.4749999999999999	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	51	1.275	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	47	1.175	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	42	1.05	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	41	1.0250000000000001	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	41	1.0250000000000001	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	37	0.9249999999999999	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	35	0.8750000000000001	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	33	0.8250000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	29	0.7250000000000001	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	28	0.7000000000000001	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	28	0.7000000000000001	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	25	0.625	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	25	0.625	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	23	0.575	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	22	0.5499999999999999	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	21	0.525	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	21	0.525	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	19	0.475	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	19	0.475	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	18	0.44999999999999996	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	17	0.42500000000000004	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	17	0.42500000000000004	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	16	0.4	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	15	0.375	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	13	0.325	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	13	0.325	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	12	0.3	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	12	0.3	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	12	0.3	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	11	0.27499999999999997	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	10	0.25	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	10	0.25	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	10	0.25	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	9	0.22499999999999998	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	9	0.22499999999999998	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	9	0.22499999999999998	No Hit
CACCCATGACCATGAGAAATCTGCTCAGCGAGGAGTAGAAGTAAAGGATG	9	0.22499999999999998	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	9	0.22499999999999998	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	8	0.2	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	8	0.2	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	8	0.2	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAAT	8	0.2	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	8	0.2	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	8	0.2	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	7	0.17500000000000002	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	7	0.17500000000000002	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	7	0.17500000000000002	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	7	0.17500000000000002	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	7	0.17500000000000002	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	7	0.17500000000000002	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	7	0.17500000000000002	No Hit
CTTGCGGATGCCATCTTCTTCCACTTCGTAGTAGTCAGGCCCACAGGCTC	6	0.15	No Hit
AGGCCAGTCGGAGTGCATGGACCTTTTGCATTTCCTCACCGGAGAAGCTA	6	0.15	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	6	0.15	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	6	0.15	No Hit
TACTTAGTGACAGCCTTGGTGCCCTCCGACACGGCGTGCTTGGCCAGCTC	6	0.15	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	6	0.15	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	6	0.15	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	6	0.15	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	6	0.15	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	6	0.15	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	6	0.15	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	6	0.15	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	6	0.15	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	5	0.125	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	5	0.125	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	5	0.125	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	5	0.125	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	5	0.125	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	5	0.125	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	5	0.125	No Hit
CCCGGTCCAGGATCTTGAAGCTCGTGTCCAGTTTCAGAGGCTGGCTACAG	5	0.125	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	5	0.125	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	5	0.125	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
CTGCCCGAGATGCTCATGTGCTCCTGCTCACTCAACATCTCTGGCCGTTC	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	5	0.125	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGC	5	0.125	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	5	0.125	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTGCCCACTGGCGATGCATGC	5	0.125	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGTCC	20	6.952051E-4	44.000004	44
ATCTGTC	20	6.952051E-4	44.000004	43
TGTTGAA	25	3.6958358E-5	44.0	6
GTTGAAA	25	3.6958358E-5	44.0	7
GCCTTAC	25	3.6958358E-5	44.0	32
CACACAG	25	3.6958358E-5	44.0	14
GAAACAC	25	3.6958358E-5	44.0	10
CCTGTTG	25	3.6958358E-5	44.0	4
TGGTAAT	25	3.6958358E-5	44.0	22
AAACACA	25	3.6958358E-5	44.0	11
GCTGGTA	25	3.6958358E-5	44.0	20
TACGGCC	25	3.6958358E-5	44.0	28
GAATGCC	25	3.6958358E-5	44.0	39
ACACAGC	25	3.6958358E-5	44.0	15
ACACACA	25	3.6958358E-5	44.0	13
AGCAGGT	25	3.6958358E-5	44.0	19
CTGGTAA	25	3.6958358E-5	44.0	21
GGCCTTA	25	3.6958358E-5	44.0	31
CCACCAA	25	3.6958358E-5	44.0	44
ATACGGC	25	3.6958358E-5	44.0	27
>>END_MODULE
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
Read 611063 spots for SRR25943846.sra
Written 611063 spots for SRR25943846.sra
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
Read 611058 spots for SRR25943846.sra
Written 611058 spots for SRR25943846.sra
SRR ids: ['SRR25943846.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9a3d98w5
SRR25943846.sra spots: 4888469
blocks: [[1, 611058], [611059, 1222116], [1222117, 1833174], [1833175, 2444232], [2444233, 3055290], [3055291, 3666348], [3666349, 4277406], [4277407, 4888469]]
SRR25943846 file size 844824
SRR25943846 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943846 SRR25943846_1.fastq
Input file:	SRR25943846_1.fastq
trimmed:	SRR25943846-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:36:04 2026 >> started

Tue May  5 05:36:05 2026 >> done (1.428s)
4888469 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
4888469 (100.00%) reads available; of these:
     97 ( 0.00%) trimmed reads available after processing
4888372 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      5	  0.00%
 44	      1	  0.00%
 45	      2	  0.00%
 46	     16	  0.00%
 47	     11	  0.00%
 48	     25	  0.00%
 49	     36	  0.00%
 50	4888372	100.00%
4888469 reads passed initial QC


criterion=sequence-density
sequence-density=18.61
sequence-density-rank=1
fanout-score=1.29
fanout-score-rank=8
prefix-density=0.04
prefix-fanout=1.3
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGT


criterion=fanout-score
sequence-density=0.64
sequence-density-rank=23
fanout-score=38.63
fanout-score-rank=1
prefix-density=24.48
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:36:13
                             Started mapping on |	May 05 05:36:13
                                    Finished on |	May 05 05:36:22
       Mapping speed, Million of reads per hour |	1955.39

                          Number of input reads |	4888469
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2996061
                        Uniquely mapped reads % |	61.29%
                          Average mapped length |	49.61
                       Number of splices: Total |	1692
            Number of splices: Annotated (sjdb) |	684
                       Number of splices: GT/AG |	1528
                       Number of splices: GC/AG |	37
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	124
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.07%
                        Deletion average length |	1.09
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	577112
             % of reads mapped to multiple loci |	11.81%
        Number of reads mapped to too many loci |	1145226
             % of reads mapped to too many loci |	23.43%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.48%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1315296	1315296	1315296
N_multimapping	577112	577112	577112
N_noFeature	3112	2887085	3145
N_ambiguous	131779	32	24001
UnstrandedReadsAssigned:2861170 PositiveStrandReadsAssigned:108944 NegativeStrandReadsAssigned:2968915
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943846 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943846-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,888,469 reads, 4,540,270 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,168 rounds

 109283 SRR25943846.ke.tsv
  52636 SRR25943846.se.tsv
 161919 total
==> SRR25943846.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943846.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943846 completed mapping pipeline successfully
