Starting /dee2/code/volunteer_pipeline.sh SRR25943805
    current disk space = 1263818420
    free memory = 195067172 
SRR25943805 SRAfilesize
e863e0f409b5da72656fdf402f4719b5  SRR25943805.sra
SRR25943805.sra file validated
SRR25943805 is single end
SRR25943805 is conventional basespace
SRR25943805 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.393	32.0	32.0	32.0	32.0	32.0
2	31.4985	32.0	32.0	32.0	32.0	32.0
3	31.667	32.0	32.0	32.0	32.0	32.0
4	31.69275	32.0	32.0	32.0	32.0	32.0
5	31.64475	32.0	32.0	32.0	32.0	32.0
6	35.198	36.0	36.0	36.0	36.0	36.0
7	35.135	36.0	36.0	36.0	36.0	36.0
8	34.953	36.0	36.0	36.0	32.0	36.0
9	34.92825	36.0	36.0	36.0	32.0	36.0
10	35.0485	36.0	36.0	36.0	32.0	36.0
11	35.1535	36.0	36.0	36.0	36.0	36.0
12	35.19275	36.0	36.0	36.0	36.0	36.0
13	35.16325	36.0	36.0	36.0	36.0	36.0
14	35.149	36.0	36.0	36.0	36.0	36.0
15	35.01375	36.0	36.0	36.0	36.0	36.0
16	34.9815	36.0	36.0	36.0	36.0	36.0
17	34.93375	36.0	36.0	36.0	36.0	36.0
18	34.98975	36.0	36.0	36.0	36.0	36.0
19	35.092	36.0	36.0	36.0	36.0	36.0
20	35.115	36.0	36.0	36.0	36.0	36.0
21	35.169	36.0	36.0	36.0	36.0	36.0
22	35.0005	36.0	36.0	36.0	36.0	36.0
23	35.021	36.0	36.0	36.0	36.0	36.0
24	35.0745	36.0	36.0	36.0	36.0	36.0
25	35.16225	36.0	36.0	36.0	36.0	36.0
26	34.76575	36.0	36.0	36.0	32.0	36.0
27	35.0195	36.0	36.0	36.0	36.0	36.0
28	34.808	36.0	36.0	36.0	32.0	36.0
29	35.03025	36.0	36.0	36.0	36.0	36.0
30	34.9305	36.0	36.0	36.0	36.0	36.0
31	35.0705	36.0	36.0	36.0	36.0	36.0
32	34.944	36.0	36.0	36.0	32.0	36.0
33	34.9115	36.0	36.0	36.0	32.0	36.0
34	34.81375	36.0	36.0	36.0	32.0	36.0
35	34.89425	36.0	36.0	36.0	36.0	36.0
36	34.86575	36.0	36.0	36.0	32.0	36.0
37	34.9775	36.0	36.0	36.0	32.0	36.0
38	34.8085	36.0	36.0	36.0	32.0	36.0
39	34.9705	36.0	36.0	36.0	32.0	36.0
40	34.5735	36.0	36.0	36.0	32.0	36.0
41	34.88025	36.0	36.0	36.0	32.0	36.0
42	34.747	36.0	36.0	36.0	32.0	36.0
43	34.68875	36.0	36.0	36.0	32.0	36.0
44	34.775	36.0	36.0	36.0	32.0	36.0
45	34.498	36.0	36.0	36.0	32.0	36.0
46	34.48375	36.0	36.0	36.0	32.0	36.0
47	34.37175	36.0	36.0	36.0	32.0	36.0
48	34.1235	36.0	36.0	36.0	32.0	36.0
49	34.43325	36.0	36.0	36.0	32.0	36.0
50	34.07475	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	-0.0011929022317218596
11101	2	0.067548088871213
11101	3	-0.08059545703066462
11101	4	-0.12170087976539534
11101	5	-0.010040260450320915
11101	6	-0.4221134251205356
11101	7	0.07490431930016683
11101	8	0.11720264426661231
11101	9	0.7157661911625866
11101	10	0.2772006560962268
11101	11	0.3107510313633881
11101	12	0.45449575028579403
11101	13	-0.021347979521841864
11101	14	0.2184999254436093
11101	15	0.043342114419210986
11101	16	0.028505392912173022
11101	17	-0.05981907649486118
11101	18	-0.3730304687111712
11101	19	-0.25461007008300385
11101	20	-0.4001192902231665
11101	21	0.14908792683532823
11101	22	0.24675679705750753
11101	23	0.08857299070530189
11101	24	0.053109001441427495
11101	25	0.19749987573935357
11101	26	0.4947810527362222
11101	27	-0.04615040508971191
11101	28	-0.2351508524280561
11101	29	-0.06603210895173817
11101	30	-0.06536110144639196
11101	31	-0.4937869675431159
11101	32	0.6141458322978295
11101	33	-0.006014215418261415
11101	34	-0.13686067896018983
11101	35	0.27593319747502676
11101	36	-0.376410358367707
11101	37	-0.20013420150106498
11101	38	-0.376087280679954
11101	39	-0.0940404592673616
11101	40	-0.34631442914657384
11101	41	0.058203688056067904
11101	42	-0.5220935434166734
11101	43	0.09314578259357376
11101	44	-0.258959192802827
11101	45	0.06886525175207936
11101	46	0.5411551269943828
11101	47	0.15281574630945727
11101	48	0.019757443212881753
11101	49	-0.057284159252454
11101	50	0.7494408270788782
11102	1	0.001192902231718307
11102	2	-0.06754808887121655
11102	3	0.08059545703066817
11102	4	0.12170087976539534
11102	5	0.010040260450320915
11102	6	0.4221134251205285
11102	7	-0.07490431930016683
11102	8	-0.11720264426661231
11102	9	-0.7157661911625794
11102	10	-0.2772006560962268
11102	11	-0.3107510313633881
11102	12	-0.45449575028580114
11102	13	0.02134797952184897
11102	14	-0.2184999254436093
11102	15	-0.04334211441920388
11102	16	-0.028505392912173022
11102	17	0.05981907649485407
11102	18	0.3730304687111641
11102	19	0.25461007008300385
11102	20	0.4001192902231736
11102	21	-0.14908792683532823
11102	22	-0.24675679705750753
11102	23	-0.08857299070530189
11102	24	-0.05310900144142039
11102	25	-0.19749987573934646
11102	26	-0.4947810527362151
11102	27	0.046150405089719015
11102	28	0.2351508524280561
11102	29	0.06603210895173817
11102	30	0.06536110144639906
11102	31	0.493786967543123
11102	32	-0.6141458322978295
11102	33	0.006014215418261415
11102	34	0.13686067896018983
11102	35	-0.27593319747501965
11102	36	0.3764103583677141
11102	37	0.20013420150107208
11102	38	0.376087280679954
11102	39	0.09404045926735449
11102	40	0.34631442914658095
11102	41	-0.0582036880560608
11102	42	0.5220935434166734
11102	43	-0.09314578259356665
11102	44	0.25895919280281987
11102	45	-0.06886525175207225
11102	46	-0.5411551269943828
11102	47	-0.15281574630945727
11102	48	-0.019757443212881753
11102	49	0.0572841592524469
11102	50	-0.7494408270788853
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	3.0
26	13.0
27	23.0
28	49.0
29	52.0
30	83.0
31	130.0
32	188.0
33	310.0
34	592.0
35	2555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.3	21.875	24.2	27.625
2	23.400000000000002	18.075	26.35	32.175
3	26.575	24.474999999999998	23.1	25.85
4	24.125	23.25	27.150000000000002	25.474999999999998
5	17.175	27.175	25.224999999999998	30.425
6	30.055055055055057	24.7997997997998	22.52252252252252	22.62262262262262
7	27.650000000000002	20.0	30.775000000000002	21.575
8	23.075000000000003	19.625	19.05	38.25
9	24.825	16.875	26.575	31.724999999999998
10	25.624999999999996	20.45	25.974999999999998	27.950000000000003
11	25.6	21.075	21.075	32.25
12	29.125	16.775000000000002	22.875	31.225
13	27.725	25.900000000000002	24.224999999999998	22.15
14	21.224999999999998	19.0	26.200000000000003	33.575
15	19.925	25.900000000000002	20.7	33.475
16	20.825	25.0	28.975	25.2
17	19.175	20.150000000000002	29.975	30.7
18	23.474999999999998	15.275	37.6	23.65
19	24.0	21.675	28.775000000000002	25.55
20	27.175	14.85	25.6	32.375
21	20.075000000000003	21.425	27.175	31.324999999999996
22	18.35	20.925	33.050000000000004	27.675
23	28.375	13.825000000000001	24.375	33.425
24	27.750000000000004	12.675	22.6	36.975
25	2.4250000000000003	6.175	76.075	15.325
26	17.599999999999998	18.475	22.6	41.325
27	26.375	21.725	25.074999999999996	26.825
28	15.75	21.725	30.625000000000004	31.900000000000002
29	31.075000000000003	20.825	20.925	27.175
30	29.049999999999997	20.025000000000002	23.05	27.875
31	24.725	25.424999999999997	22.900000000000002	26.950000000000003
32	21.95	20.225	22.8	35.025
33	31.75	21.675	19.650000000000002	26.924999999999997
34	24.825	17.549999999999997	33.6	24.025
35	23.925	22.275	23.05	30.75
36	26.974999999999998	16.275000000000002	21.425	35.325
37	20.025000000000002	28.225	26.450000000000003	25.3
38	27.675	13.750000000000002	24.85	33.725
39	31.0	21.825	19.950000000000003	27.224999999999998
40	23.35	25.525	24.675	26.450000000000003
41	22.125	23.525	20.5	33.85
42	29.849999999999998	23.5	18.825	27.825
43	24.6	23.05	24.675	27.675
44	25.525	19.8	22.35	32.324999999999996
45	18.2	26.8	21.2	33.800000000000004
46	22.95	22.975	27.6	26.474999999999998
47	25.275	19.725	18.475	36.525
48	22.83070767691923	25.431357839459867	23.1807951987997	28.557139284821204
49	26.224999999999998	17.849999999999998	31.624999999999996	24.3
50	18.675	15.225	20.925	45.175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	2.5
32	4.0
33	2.5
34	1.0
35	5.0
36	9.0
37	16.5
38	24.0
39	36.5
40	49.0
41	106.5
42	164.0
43	119.5
44	75.0
45	146.0
46	217.0
47	245.0
48	273.0
49	414.0
50	555.0
51	581.0
52	607.0
53	475.0
54	343.0
55	446.5
56	550.0
57	497.5
58	445.0
59	364.0
60	283.0
61	207.0
62	131.0
63	107.0
64	83.0
65	76.5
66	70.0
67	58.0
68	46.0
69	42.0
70	38.0
71	33.0
72	28.0
73	14.5
74	1.0
75	1.5
76	2.0
77	1.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.025
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21839841164793	26.525
2	11.118464592984779	8.4
3	6.353408338848444	7.199999999999999
4	3.6399735274652545	5.5
5	1.6545334215751157	3.125
6	1.0589013898080741	2.4
7	0.6618133686300464	1.7500000000000002
8	0.7941760423560555	2.4
9	0.6618133686300464	2.25
>10	3.507610853739246	29.925
>50	0.26472534745201853	6.950000000000001
>100	0.06618133686300463	3.5749999999999997
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	143	3.5749999999999997	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	82	2.0500000000000003	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	70	1.7500000000000002	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	66	1.6500000000000001	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	60	1.5	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	49	1.225	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTC	44	1.0999999999999999	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	40	1.0	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	40	1.0	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	38	0.95	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	36	0.8999999999999999	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	35	0.8750000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	35	0.8750000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	35	0.8750000000000001	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	34	0.8500000000000001	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	32	0.8	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	31	0.775	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	29	0.7250000000000001	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	28	0.7000000000000001	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	28	0.7000000000000001	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATAT	27	0.675	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	27	0.675	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	27	0.675	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	27	0.675	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	26	0.65	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	26	0.65	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	26	0.65	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	26	0.65	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	24	0.6	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	24	0.6	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	23	0.575	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	21	0.525	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	20	0.5	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	19	0.475	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	18	0.44999999999999996	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	18	0.44999999999999996	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	18	0.44999999999999996	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	18	0.44999999999999996	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	17	0.42500000000000004	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	16	0.4	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	15	0.375	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	15	0.375	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	14	0.35000000000000003	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	14	0.35000000000000003	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	13	0.325	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	13	0.325	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	12	0.3	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	12	0.3	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	12	0.3	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	12	0.3	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTC	11	0.27499999999999997	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	11	0.27499999999999997	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	11	0.27499999999999997	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	10	0.25	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	10	0.25	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	10	0.25	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	10	0.25	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	10	0.25	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	9	0.22499999999999998	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	9	0.22499999999999998	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	9	0.22499999999999998	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	9	0.22499999999999998	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	9	0.22499999999999998	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	9	0.22499999999999998	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	9	0.22499999999999998	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	9	0.22499999999999998	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	9	0.22499999999999998	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	9	0.22499999999999998	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	8	0.2	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	8	0.2	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	8	0.2	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	8	0.2	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	8	0.2	No Hit
TGGAAGGACACTGGTCAGCAATCCTCAACACAGCACGCCACTTCCCAAAG	8	0.2	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGAC	8	0.2	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	8	0.2	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	8	0.2	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	8	0.2	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	8	0.2	No Hit
CTGTGTTCTCCCTACAACTGTGTGTGTGATCCTCCTCAATGCTTCCCTCT	8	0.2	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	7	0.17500000000000002	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	7	0.17500000000000002	No Hit
CACTGGCACTGAGCCAAGGCAGCTTTGATGGCGGTCACCAGTTTCCGGAA	7	0.17500000000000002	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	7	0.17500000000000002	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	7	0.17500000000000002	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	7	0.17500000000000002	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	7	0.17500000000000002	No Hit
ACAATGGCGATCTGCCGGTGCACATCAGCTTGAGAGAAGTCGGCACGGCC	7	0.17500000000000002	No Hit
CCTGTGTCCTGAGTGCTGGAGGCTTCCTTAGTGAGGCCAAAGTCACTGAC	6	0.15	No Hit
TCAAGGTGTAGAGTGAAGTCCTCCTTAGCTGTCCGGATATTCAAGGATGC	6	0.15	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	6	0.15	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTC	6	0.15	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	6	0.15	No Hit
CATGTGGCGAAGACCTCACAAGCTTTTCAACGTAAGGGACAGGAATCATC	6	0.15	No Hit
CACTGGTTTGCCTGATTAAGCTGTTCACACCCAGAACGGTAGCAGCTGCA	6	0.15	No Hit
AGGTGAAGTAGTAGAGGCCAGGCACCTTGCAGGTGAACTTGCCGTTGCGT	6	0.15	No Hit
GAGCCTGATCGGAAGTCCATCGTCTCTTCTTCCTGCCTCTTGAGTCCATC	6	0.15	No Hit
TGCACTTCCACAGCCTTGCCACTGTCACTTTCATCACTGTCATGGCAAGC	6	0.15	No Hit
TTGAAAGCAACCCAACAGGACCGGTCACACACTCTCCACCAAGTTTAAAA	6	0.15	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	6	0.15	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	6	0.15	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	6	0.15	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	6	0.15	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	5	0.125	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGT	5	0.125	No Hit
GCAGACGCAACTCTCGGTAGGTCCTTTTGGCGTGAATGATGGACTGAAAC	5	0.125	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	5	0.125	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	5	0.125	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	5	0.125	No Hit
CCTTAAAGGTGTTCAGTTTGTGGTTAGCAATGCGCCCAGCCACCACAATC	5	0.125	No Hit
GATCCAGGTCTGAATGTCTATTGGTTCAGCTTTGGGATAAGCTATGGTTG	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	5	0.125	No Hit
TCATGCAAGAACTCACACTGGTCCCCTTTCTTGCACAGTCCTCTCAGCCA	5	0.125	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	5	0.125	No Hit
TCAACCTCTAGCCGCTGATTGCGCTGGTCCAGAGAGCCACGAAGGACATC	5	0.125	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	5	0.125	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	5	0.125	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	5	0.125	No Hit
TGTCTGGCTTTCAGGCTCAGGCACCTTCCAGTCCATCTTTCGGCCCTTCT	5	0.125	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	5	0.125	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	5	0.125	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	5	0.125	No Hit
CGGCGCCAATTCCTTTCACCGAGTTCGCGAGGCAAGACGCGGAGACTCGC	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	5	0.125	No Hit
CTGTTTCCTGCAGTCCAGGCGAGATTGACAGCAGTCTCCAACTTCTTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCC	20	6.952051E-4	44.000004	29
AGCTTGC	20	6.952051E-4	44.000004	31
ATCTTCT	20	6.952051E-4	44.000004	15
ACAGAGG	20	6.952051E-4	44.000004	3
CCTGTAT	20	6.952051E-4	44.000004	10
GTATCTT	20	6.952051E-4	44.000004	13
GGTTTGC	20	6.952051E-4	44.000004	28
CATCAGG	20	6.952051E-4	44.000004	23
CAGAGGC	20	6.952051E-4	44.000004	4
ATCAGGT	20	6.952051E-4	44.000004	24
GGCCTGT	20	6.952051E-4	44.000004	8
TTCTCCA	20	6.952051E-4	44.000004	18
AGGCCTG	20	6.952051E-4	44.000004	7
CCATCAG	20	6.952051E-4	44.000004	22
TCCATCA	20	6.952051E-4	44.000004	21
AGGTTTG	20	6.952051E-4	44.000004	27
CAGGTTT	20	6.952051E-4	44.000004	26
GCCTGTA	20	6.952051E-4	44.000004	9
CTGTATC	20	6.952051E-4	44.000004	11
CACAGAG	20	6.952051E-4	44.000004	2
>>END_MODULE
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349493 spots for SRR25943805.sra
Written 349493 spots for SRR25943805.sra
Read 349499 spots for SRR25943805.sra
Written 349499 spots for SRR25943805.sra
SRR ids: ['SRR25943805.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x1vdsrmt
SRR25943805.sra spots: 2795950
blocks: [[1, 349493], [349494, 698986], [698987, 1048479], [1048480, 1397972], [1397973, 1747465], [1747466, 2096958], [2096959, 2446451], [2446452, 2795950]]
SRR25943805 file size 482736
SRR25943805 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943805 SRR25943805_1.fastq
Input file:	SRR25943805_1.fastq
trimmed:	SRR25943805-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:45:55 2026 >> started

Tue May  5 05:45:55 2026 >> done (0.855s)
2795950 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2795950 (100.00%) reads available; of these:
     65 ( 0.00%) trimmed reads available after processing
2795885 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      1	  0.00%
 44	      2	  0.00%
 45	      0	  0.00%
 46	     12	  0.00%
 47	      9	  0.00%
 48	      8	  0.00%
 49	     33	  0.00%
 50	2795885	100.00%
2795950 reads passed initial QC


criterion=sequence-density
sequence-density=5.07
sequence-density-rank=1
fanout-score=10.92
fanout-score-rank=1
prefix-density=5.21
prefix-fanout=10.6
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTCGTATGC


criterion=fanout-score
sequence-density=5.07
sequence-density-rank=1
fanout-score=10.92
fanout-score-rank=1
prefix-density=5.21
prefix-fanout=10.6
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTCGTATGC -o SRR25943805 -
Input file:	STDIN
trimmed:	SRR25943805-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTCGTATGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Tue May  5 05:45:59 2026 >> started

Tue May  5 05:46:00 2026 >> done (1.065s)
1863967 reads processed; of these:
  64962 ( 3.49%) short reads filtered out after trimming by size control
   3755 ( 0.20%) empty reads filtered out after trimming by size control
1795250 (96.31%) reads available; of these:
 102269 ( 5.70%) trimmed reads available after processing
1692981 (94.30%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    257	  0.01%
 19	    300	  0.02%
 20	    394	  0.02%
 21	    381	  0.02%
 22	    595	  0.03%
 23	    791	  0.04%
 24	   1317	  0.07%
 25	   2133	  0.12%
 26	   1772	  0.10%
 27	   1888	  0.11%
 28	   1919	  0.11%
 29	   1745	  0.10%
 30	   1884	  0.10%
 31	   2850	  0.16%
 32	   1763	  0.10%
 33	   1796	  0.10%
 34	   1694	  0.09%
 35	   1677	  0.09%
 36	   1582	  0.09%
 37	   1516	  0.08%
 38	   1995	  0.11%
 39	   2067	  0.12%
 40	   1492	  0.08%
 41	   1623	  0.09%
 42	   2161	  0.12%
 43	   1818	  0.10%
 44	   2194	  0.12%
 45	   3478	  0.19%
 46	  29684	  1.65%
 47	  27518	  1.53%
 48	      7	  0.00%
 49	     22	  0.00%
 50	1692937	 94.30%


criterion=sequence-density
sequence-density=4.10
sequence-density-rank=1
fanout-score=1.49
fanout-score-rank=9
prefix-density=0.09
prefix-fanout=1.5
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC


criterion=fanout-score
sequence-density=0.52
sequence-density-rank=35
fanout-score=5.86
fanout-score-rank=1
prefix-density=0.56
prefix-fanout=5.5
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTCGTATGC
                                 Started job on |	May 05 05:46:06
                             Started mapping on |	May 05 05:46:06
                                    Finished on |	May 05 05:46:11
       Mapping speed, Million of reads per hour |	1963.61

                          Number of input reads |	2727233
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2220586
                        Uniquely mapped reads % |	81.42%
                          Average mapped length |	49.28
                       Number of splices: Total |	2772
            Number of splices: Annotated (sjdb) |	1754
                       Number of splices: GT/AG |	2330
                       Number of splices: GC/AG |	109
                       Number of splices: AT/AC |	4
               Number of splices: Non-canonical |	329
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.10%
                        Deletion average length |	1.22
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	251405
             % of reads mapped to multiple loci |	9.22%
        Number of reads mapped to too many loci |	133064
             % of reads mapped to too many loci |	4.88%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.47%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	255242	255242	255242
N_multimapping	251405	251405	251405
N_noFeature	2058	2149262	2235
N_ambiguous	91562	18	21580
UnstrandedReadsAssigned:2126966 PositiveStrandReadsAssigned:71306 NegativeStrandReadsAssigned:2196771
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943805 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943805-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,727,233 reads, 2,424,122 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,119 rounds

 109283 SRR25943805.ke.tsv
  52636 SRR25943805.se.tsv
 161919 total
==> SRR25943805.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943805.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943805 completed mapping pipeline successfully
