Starting /dee2/code/volunteer_pipeline.sh SRR25943867
    current disk space = 1259954904
    free memory = 193692056 
SRR25943867 SRAfilesize
e484432f99f83b505ca16a3f8af68e11  SRR25943867.sra
SRR25943867.sra file validated
SRR25943867 is single end
SRR25943867 is conventional basespace
SRR25943867 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44625	32.0	32.0	32.0	32.0	32.0
2	31.449	32.0	32.0	32.0	32.0	32.0
3	31.59	32.0	32.0	32.0	32.0	32.0
4	31.691	32.0	32.0	32.0	32.0	32.0
5	31.65725	32.0	32.0	32.0	32.0	32.0
6	35.12725	36.0	36.0	36.0	36.0	36.0
7	35.198	36.0	36.0	36.0	36.0	36.0
8	35.06925	36.0	36.0	36.0	36.0	36.0
9	35.15975	36.0	36.0	36.0	36.0	36.0
10	35.12675	36.0	36.0	36.0	36.0	36.0
11	35.11675	36.0	36.0	36.0	36.0	36.0
12	35.20775	36.0	36.0	36.0	36.0	36.0
13	35.25875	36.0	36.0	36.0	36.0	36.0
14	35.18875	36.0	36.0	36.0	36.0	36.0
15	35.21275	36.0	36.0	36.0	36.0	36.0
16	34.93975	36.0	36.0	36.0	32.0	36.0
17	35.1115	36.0	36.0	36.0	36.0	36.0
18	35.06625	36.0	36.0	36.0	36.0	36.0
19	35.26925	36.0	36.0	36.0	36.0	36.0
20	35.06675	36.0	36.0	36.0	36.0	36.0
21	35.13075	36.0	36.0	36.0	36.0	36.0
22	35.17125	36.0	36.0	36.0	36.0	36.0
23	35.09875	36.0	36.0	36.0	36.0	36.0
24	34.9795	36.0	36.0	36.0	36.0	36.0
25	34.884	36.0	36.0	36.0	36.0	36.0
26	35.0885	36.0	36.0	36.0	36.0	36.0
27	35.0425	36.0	36.0	36.0	36.0	36.0
28	34.78075	36.0	36.0	36.0	32.0	36.0
29	35.14775	36.0	36.0	36.0	36.0	36.0
30	35.0495	36.0	36.0	36.0	36.0	36.0
31	35.18275	36.0	36.0	36.0	36.0	36.0
32	35.02	36.0	36.0	36.0	36.0	36.0
33	35.20025	36.0	36.0	36.0	36.0	36.0
34	35.12725	36.0	36.0	36.0	36.0	36.0
35	35.0945	36.0	36.0	36.0	36.0	36.0
36	35.01025	36.0	36.0	36.0	36.0	36.0
37	34.981	36.0	36.0	36.0	36.0	36.0
38	35.0685	36.0	36.0	36.0	36.0	36.0
39	34.9655	36.0	36.0	36.0	36.0	36.0
40	34.84075	36.0	36.0	36.0	36.0	36.0
41	34.965	36.0	36.0	36.0	36.0	36.0
42	34.85275	36.0	36.0	36.0	32.0	36.0
43	34.7675	36.0	36.0	36.0	32.0	36.0
44	34.8375	36.0	36.0	36.0	36.0	36.0
45	34.8095	36.0	36.0	36.0	36.0	36.0
46	34.733	36.0	36.0	36.0	32.0	36.0
47	34.49775	36.0	36.0	36.0	32.0	36.0
48	34.59625	36.0	36.0	36.0	32.0	36.0
49	34.726	36.0	36.0	36.0	32.0	36.0
50	34.296	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	10.0
27	30.0
28	27.0
29	63.0
30	72.0
31	125.0
32	164.0
33	263.0
34	565.0
35	2678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.125	12.8	29.45	35.625
2	19.6	14.674999999999999	35.8	29.925
3	43.75	15.2	13.725000000000001	27.325
4	21.099999999999998	15.675	17.349999999999998	45.875
5	25.424999999999997	20.825	22.35	31.4
6	35.317658829414704	21.085542771385693	17.858929464732366	25.737868934467233
7	34.375	12.9	24.474999999999998	28.249999999999996
8	18.3	24.65	15.049999999999999	42.0
9	28.199999999999996	27.025	21.275	23.5
10	17.875	13.4	38.1	30.625000000000004
11	30.55	37.05	13.350000000000001	19.05
12	28.799999999999997	15.174999999999999	38.9	17.125
13	38.07855891918939	21.065799349512133	13.284963722792096	27.570678008506377
14	22.35	16.650000000000002	35.3	25.7
15	26.75	36.725	16.150000000000002	20.375
16	12.225	35.3	34.050000000000004	18.425
17	25.924999999999997	15.275	34.425	24.375
18	30.349999999999998	14.75	38.0	16.900000000000002
19	29.225	30.225	23.200000000000003	17.349999999999998
20	23.875	13.15	28.050000000000004	34.925
21	12.325	14.025000000000002	51.87500000000001	21.775
22	16.075	13.175	48.9	21.85
23	34.475	11.85	18.375	35.3
24	20.974999999999998	13.750000000000002	30.225	35.05
25	1.325	2.5749999999999997	77.55	18.55
26	23.9	20.925	27.075	28.1
27	35.225	19.025	16.125	29.625
28	12.45	18.175	37.724999999999994	31.65
29	21.2	29.549999999999997	12.25	37.0
30	18.224999999999998	29.425	31.775	20.575
31	39.65	17.5	14.575	28.275
32	22.0	15.275	27.075	35.65
33	23.65	29.45	24.275	22.625
34	15.174999999999999	30.099999999999998	38.5	16.225
35	18.425	12.425	39.775	29.375
36	36.0	9.85	24.75	29.4
37	32.550000000000004	32.45	19.575	15.425
38	35.099999999999994	9.25	29.2	26.450000000000003
39	36.95	19.725	15.2	28.125
40	16.0	20.275000000000002	30.5	33.225
41	15.950000000000001	29.65	16.675	37.724999999999994
42	31.33133133133133	15.315315315315313	22.722722722722725	30.630630630630627
43	19.175	17.825	29.5	33.5
44	21.05	13.0	31.7	34.25
45	10.685685685685687	34.68468468468468	23.773773773773772	30.855855855855857
46	14.549999999999999	21.725	37.175000000000004	26.55
47	35.449999999999996	10.299999999999999	14.374999999999998	39.875
48	13.488488488488489	19.51951951951952	46.77177177177177	20.22022022022022
49	23.34250688016012	16.38729046785089	29.472104078058543	30.79809857393045
50	10.424999999999999	20.025000000000002	15.25	54.300000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	6.5
30	13.0
31	7.5
32	2.0
33	1.5
34	1.0
35	4.5
36	8.0
37	11.5
38	15.0
39	34.5
40	54.0
41	125.0
42	196.0
43	238.0
44	280.0
45	232.0
46	184.0
47	166.5
48	149.0
49	342.0
50	535.0
51	690.0
52	845.0
53	528.5
54	212.0
55	252.0
56	292.0
57	531.5
58	771.0
59	527.0
60	283.0
61	184.0
62	85.0
63	61.5
64	38.0
65	26.5
66	15.0
67	12.5
68	10.0
69	8.0
70	6.0
71	6.0
72	6.0
73	3.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.075
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.1
43	0.0
44	0.0
45	0.1
46	0.0
47	0.0
48	0.1
49	0.075
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.900000000000002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1326164874552	20.125
2	12.365591397849462	6.9
3	4.480286738351254	3.75
4	2.6881720430107525	3.0
5	1.7921146953405016	2.5
6	0.8064516129032258	1.35
7	0.2688172043010753	0.525
8	0.6272401433691757	1.4000000000000001
9	0.4480286738351254	1.125
>10	3.673835125448029	20.1
>50	0.2688172043010753	4.2250000000000005
>100	0.35842293906810035	21.7
>500	0.08960573476702509	13.3
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	532	13.3	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	362	9.049999999999999	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	180	4.5	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	179	4.475	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	147	3.675	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	63	1.575	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	55	1.375	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	51	1.275	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	45	1.125	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	42	1.05	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	38	0.95	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	36	0.8999999999999999	No Hit
CTGACCAGGATGGAGGTGACATTGTAGCTATTGTTCTCTTGTAGCTCATA	35	0.8750000000000001	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	27	0.675	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	27	0.675	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	25	0.625	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	23	0.575	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	23	0.575	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	22	0.5499999999999999	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	22	0.5499999999999999	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	22	0.5499999999999999	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	21	0.525	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	21	0.525	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	20	0.5	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	20	0.5	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	19	0.475	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	19	0.475	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	19	0.475	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	18	0.44999999999999996	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	17	0.42500000000000004	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	17	0.42500000000000004	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	15	0.375	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	15	0.375	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	15	0.375	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	15	0.375	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	15	0.375	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	14	0.35000000000000003	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	14	0.35000000000000003	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	13	0.325	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	12	0.3	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	12	0.3	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	12	0.3	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	12	0.3	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	11	0.27499999999999997	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	11	0.27499999999999997	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	10	0.25	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	10	0.25	No Hit
TCGAAGACCTGGACTGTGGAATCTTTGTCAAATGAAACAGTGTTGATCAC	10	0.25	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	10	0.25	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	9	0.22499999999999998	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	9	0.22499999999999998	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	9	0.22499999999999998	No Hit
ATTCAACAATGCCAGAGGAGGACTCCCAAGTGGTACATAGGTGTACTGGA	9	0.22499999999999998	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	9	0.22499999999999998	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	8	0.2	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	8	0.2	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	8	0.2	No Hit
ACCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	8	0.2	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	8	0.2	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	8	0.2	No Hit
TGTGTCCTCTCTGAGCTTCATGTTTTCAGCAAAGACATCCGGTGCCACAC	8	0.2	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	7	0.17500000000000002	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	7	0.17500000000000002	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	7	0.17500000000000002	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	6	0.15	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	6	0.15	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	6	0.15	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	6	0.15	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCATCTC	6	0.15	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATTTTCCAGCCAGCTTCCTTCC	6	0.15	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	6	0.15	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	5	0.125	No Hit
ACATGGCTTGCATAGTACTCGTAGTTCCTCAGTGCAGCCAGGACACCGGC	5	0.125	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	5	0.125	No Hit
TAAGTACGGTCTTCATAGTGAACCTCCTTGAACAGAAGTTGAAGAGGTCT	5	0.125	No Hit
CCTTAAAGGTGTTCAGTTTGTGGTTAGCAATGCGCCCAGCCACCACAATC	5	0.125	No Hit
ACTTCTGGCACTAGGACCATAATGTCATGTCCTCGTTCACTGAGATGTTC	5	0.125	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	5	0.125	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	5	0.125	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCAGCCAGCTTCCTTCC	5	0.125	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	5	0.125	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	5	0.125	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	5	0.125	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	5	0.125	No Hit
TCACTTCGCACTTCTCAAACAATGTAAAATTGGGCTCGAACCCGCCACCA	5	0.125	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	5	0.125	No Hit
GTACTTTGACAGCTTCCACCTCATTGGCTGTTGAGTCCATCTTAGCAATG	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	5	0.125	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	5	0.125	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTC	35	1.02281774E-7	44.0	21
ATCCTAG	30	1.9515464E-6	44.0	6
AAGTATT	35	1.02281774E-7	44.0	15
GAGGTAT	30	1.9515464E-6	44.0	1
ATCTTTC	30	1.9515464E-6	44.0	29
ACATGTG	30	1.9515464E-6	44.0	16
TCCTAGG	30	1.9515464E-6	44.0	7
GGACTTC	30	1.9515464E-6	44.0	37
TTTCCAG	30	1.9515464E-6	44.0	32
TGTCTCC	35	1.02281774E-7	44.0	22
CATCTTT	30	1.9515464E-6	44.0	28
GAAGTAT	35	1.02281774E-7	44.0	14
CTCCATC	35	1.02281774E-7	44.0	25
GTATTTG	35	1.02281774E-7	44.0	17
GCGGCCA	50	1.4551915E-11	44.0	3
ATTGGAC	30	1.9515464E-6	44.0	34
CTAGGTA	30	1.9515464E-6	44.0	9
AGTATTT	35	1.02281774E-7	44.0	16
GAGCATG	35	1.02281774E-7	44.0	7
ATGTGGA	30	1.9515464E-6	44.0	18
>>END_MODULE
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
Read 522935 spots for SRR25943867.sra
Written 522935 spots for SRR25943867.sra
SRR ids: ['SRR25943867.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_wsqw48mi
SRR25943867.sra spots: 4183480
blocks: [[1, 522935], [522936, 1045870], [1045871, 1568805], [1568806, 2091740], [2091741, 2614675], [2614676, 3137610], [3137611, 3660545], [3660546, 4183480]]
SRR25943867 file size 722836
SRR25943867 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943867 SRR25943867_1.fastq
Input file:	SRR25943867_1.fastq
trimmed:	SRR25943867-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:35:31 2026 >> started

Tue May  5 05:35:33 2026 >> done (1.211s)
4183480 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
4183480 (100.00%) reads available; of these:
     96 ( 0.00%) trimmed reads available after processing
4183384 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      5	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      2	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	     15	  0.00%
 47	     10	  0.00%
 48	     17	  0.00%
 49	     47	  0.00%
 50	4183384	100.00%
4183480 reads passed initial QC


criterion=sequence-density
sequence-density=13.41
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCCCCTG


criterion=fanout-score
sequence-density=0.29
sequence-density-rank=45
fanout-score=20.88
fanout-score-rank=1
prefix-density=5.93
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:35:39
                             Started mapping on |	May 05 05:35:39
                                    Finished on |	May 05 05:35:47
       Mapping speed, Million of reads per hour |	1882.57

                          Number of input reads |	4183480
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3306485
                        Uniquely mapped reads % |	79.04%
                          Average mapped length |	49.57
                       Number of splices: Total |	1860
            Number of splices: Annotated (sjdb) |	774
                       Number of splices: GT/AG |	1649
                       Number of splices: GC/AG |	34
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	177
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.07%
                        Deletion average length |	1.08
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	325821
             % of reads mapped to multiple loci |	7.79%
        Number of reads mapped to too many loci |	456741
             % of reads mapped to too many loci |	10.92%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.26%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	551174	551174	551174
N_multimapping	325821	325821	325821
N_noFeature	4247	3264197	4256
N_ambiguous	63375	58	21681
UnstrandedReadsAssigned:3238863 PositiveStrandReadsAssigned:42230 NegativeStrandReadsAssigned:3280548
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943867 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943867-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,183,480 reads, 3,932,705 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,120 rounds

 109283 SRR25943867.ke.tsv
  52636 SRR25943867.se.tsv
 161919 total
==> SRR25943867.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943867.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943867 completed mapping pipeline successfully
