Starting /dee2/code/volunteer_pipeline.sh SRR25943861
    current disk space = 489446520832
    free memory = 365338052 
SRR25943861 SRAfilesize
6c5bb98edbf74e172149e1ea2ebb71cd  SRR25943861.sra
SRR25943861.sra file validated
SRR25943861 is single end
SRR25943861 is conventional basespace
SRR25943861 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47425	32.0	32.0	32.0	32.0	32.0
2	31.4795	32.0	32.0	32.0	32.0	32.0
3	31.64625	32.0	32.0	32.0	32.0	32.0
4	31.65075	32.0	32.0	32.0	32.0	32.0
5	31.693	32.0	32.0	32.0	32.0	32.0
6	35.05925	36.0	36.0	36.0	36.0	36.0
7	35.21475	36.0	36.0	36.0	36.0	36.0
8	35.0245	36.0	36.0	36.0	36.0	36.0
9	35.0605	36.0	36.0	36.0	36.0	36.0
10	35.1335	36.0	36.0	36.0	36.0	36.0
11	35.17125	36.0	36.0	36.0	36.0	36.0
12	35.20075	36.0	36.0	36.0	36.0	36.0
13	35.21175	36.0	36.0	36.0	36.0	36.0
14	35.14625	36.0	36.0	36.0	36.0	36.0
15	35.0685	36.0	36.0	36.0	36.0	36.0
16	35.051	36.0	36.0	36.0	36.0	36.0
17	34.99775	36.0	36.0	36.0	36.0	36.0
18	35.0845	36.0	36.0	36.0	36.0	36.0
19	35.03575	36.0	36.0	36.0	36.0	36.0
20	35.095	36.0	36.0	36.0	36.0	36.0
21	35.0875	36.0	36.0	36.0	36.0	36.0
22	35.04925	36.0	36.0	36.0	36.0	36.0
23	35.14725	36.0	36.0	36.0	36.0	36.0
24	35.03125	36.0	36.0	36.0	36.0	36.0
25	35.2435	36.0	36.0	36.0	36.0	36.0
26	34.94875	36.0	36.0	36.0	36.0	36.0
27	34.886	36.0	36.0	36.0	36.0	36.0
28	34.83775	36.0	36.0	36.0	32.0	36.0
29	34.945	36.0	36.0	36.0	36.0	36.0
30	34.99675	36.0	36.0	36.0	36.0	36.0
31	35.07125	36.0	36.0	36.0	36.0	36.0
32	34.93075	36.0	36.0	36.0	32.0	36.0
33	34.86225	36.0	36.0	36.0	32.0	36.0
34	34.95875	36.0	36.0	36.0	36.0	36.0
35	35.06525	36.0	36.0	36.0	36.0	36.0
36	34.9925	36.0	36.0	36.0	36.0	36.0
37	34.865	36.0	36.0	36.0	32.0	36.0
38	34.935	36.0	36.0	36.0	36.0	36.0
39	35.00425	36.0	36.0	36.0	36.0	36.0
40	34.675	36.0	36.0	36.0	32.0	36.0
41	34.77975	36.0	36.0	36.0	32.0	36.0
42	34.874	36.0	36.0	36.0	36.0	36.0
43	34.58375	36.0	36.0	36.0	32.0	36.0
44	34.745	36.0	36.0	36.0	32.0	36.0
45	34.476	36.0	36.0	36.0	32.0	36.0
46	34.36475	36.0	36.0	36.0	32.0	36.0
47	34.42925	36.0	36.0	36.0	32.0	36.0
48	34.287	36.0	36.0	36.0	32.0	36.0
49	34.51975	36.0	36.0	36.0	32.0	36.0
50	34.16125	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0011140819964339244
11101	2	0.10550356506238856
11101	3	-0.01336898395721775
11101	4	-0.12310606060605878
11101	5	0.11296791443850296
11101	6	0.21512923351158975
11101	7	-0.008578431372548323
11101	8	0.028074866310163316
11101	9	-0.15095811051693886
11101	10	0.05759803921568363
11101	11	0.18248663101604023
11101	12	0.21691176470588402
11101	13	-0.018605169340460748
11101	14	0.4213458110516868
11101	15	0.04155525846702801
11101	16	-0.16187611408199842
11101	17	0.24654634581105483
11101	18	0.20042335115864773
11101	19	0.33422459893048284
11101	20	0.27161319073083234
11101	21	0.02350713012477712
11101	22	0.40118092691622564
11101	23	0.23573975044563156
11101	24	0.09246880570410099
11101	25	-0.11085115864528206
11101	26	0.072972370766486
11101	27	0.34625668449197633
11101	28	0.4386140819964339
11101	29	0.03386809269162683
11101	30	0.2667112299465231
11101	31	-0.08990641711229586
11101	32	0.2037655971479495
11101	33	-0.08556149732620355
11101	34	0.059380570409977906
11101	35	0.28799019607843235
11101	36	0.3069295900178304
11101	37	-0.08667557932263747
11101	38	0.1309046345811069
11101	39	-0.29979946524063905
11101	40	0.15118092691622564
11101	41	0.15407754010694674
11101	42	-0.022393048128343196
11101	43	0.07007575757575779
11101	44	-0.06651069518716923
11101	45	0.15875668449197633
11101	46	0.42903297682709507
11101	47	0.006127450980393689
11101	48	0.07976827094474004
11101	49	0.4891934046345767
11101	50	0.2156862745097996
11102	1	-0.0011140819964339244
11102	2	-0.10550356506238856
11102	3	0.013368983957221303
11102	4	0.12310606060606233
11102	5	-0.11296791443850296
11102	6	-0.21512923351158264
11102	7	0.008578431372555428
11102	8	-0.02807486631015621
11102	9	0.15095811051693175
11102	10	-0.05759803921568363
11102	11	-0.18248663101604023
11102	12	-0.21691176470588402
11102	13	0.018605169340467853
11102	14	-0.4213458110516939
11102	15	-0.041555258467020906
11102	16	0.16187611408199132
11102	17	-0.24654634581104773
11102	18	-0.20042335115864063
11102	19	-0.33422459893047574
11102	20	-0.27161319073083945
11102	21	-0.02350713012477712
11102	22	-0.40118092691621854
11102	23	-0.23573975044563156
11102	24	-0.09246880570410099
11102	25	0.11085115864527495
11102	26	-0.0729723707664931
11102	27	-0.34625668449198344
11102	28	-0.43861408199644103
11102	29	-0.03386809269161972
11102	30	-0.2667112299465231
11102	31	0.08990641711230296
11102	32	-0.2037655971479566
11102	33	0.08556149732620355
11102	34	-0.05938057040998501
11102	35	-0.28799019607843235
11102	36	-0.3069295900178233
11102	37	0.08667557932263747
11102	38	-0.1309046345810998
11102	39	0.29979946524063905
11102	40	-0.15118092691621854
11102	41	-0.15407754010695385
11102	42	0.022393048128343196
11102	43	-0.07007575757575779
11102	44	0.06651069518716213
11102	45	-0.15875668449198344
11102	46	-0.42903297682709507
11102	47	-0.006127450980386584
11102	48	-0.07976827094474004
11102	49	-0.48919340463458383
11102	50	-0.2156862745098067
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	4.0
25	9.0
26	9.0
27	21.0
28	33.0
29	61.0
30	100.0
31	116.0
32	173.0
33	274.0
34	614.0
35	2584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.525	19.575	22.975	28.925
2	24.65	17.8	29.099999999999998	28.449999999999996
3	31.075000000000003	22.325	22.400000000000002	24.2
4	30.55	21.2	24.975	23.275000000000002
5	14.674999999999999	29.099999999999998	27.800000000000004	28.425
6	33.19159579789895	21.76088044022011	23.761880940470235	21.285642821410704
7	33.15	17.1	31.2	18.55
8	22.1	18.15	20.075000000000003	39.675
9	22.0	18.45	30.125	29.425
10	26.25	17.7	24.224999999999998	31.825
11	25.6	29.45	18.575	26.375
12	35.25	17.724999999999998	19.950000000000003	27.075
13	34.699999999999996	26.650000000000002	19.2	19.45
14	20.525	20.825	23.375	35.275
15	17.299999999999997	26.75	22.0	33.95
16	18.35	33.800000000000004	25.174999999999997	22.675
17	19.875	19.425	30.925000000000004	29.775000000000002
18	20.65	13.025	42.975	23.35
19	21.6	23.075000000000003	27.1	28.225
20	28.175	14.075	28.549999999999997	29.2
21	18.425	21.275	31.5	28.799999999999997
22	18.675	21.099999999999998	31.874999999999996	28.349999999999998
23	27.975	18.25	25.3	28.475
24	27.700000000000003	12.425	26.325	33.550000000000004
25	1.55	5.75	81.69999999999999	11.0
26	14.374999999999998	19.475	20.549999999999997	45.6
27	26.325	20.65	26.275	26.75
28	14.499999999999998	20.875	30.675	33.95
29	38.05	20.5	16.85	24.6
30	31.324999999999996	17.75	22.05	28.875
31	30.875000000000004	25.1	15.975	28.050000000000004
32	21.775	19.175	22.5	36.55
33	31.374999999999996	21.5	16.150000000000002	30.975
34	24.5	17.8	36.375	21.325
35	28.975	21.3	23.599999999999998	26.125
36	33.074999999999996	14.000000000000002	24.325	28.599999999999998
37	21.075	31.2	25.775	21.95
38	31.674999999999997	12.425	22.125	33.775
39	33.4	22.5	18.224999999999998	25.874999999999996
40	20.925	26.1	21.775	31.2
41	20.200000000000003	27.825	20.225	31.75
42	33.4	20.5	20.375	25.724999999999998
43	23.825	23.325000000000003	23.65	29.2
44	29.2	15.975	22.975	31.85
45	13.625000000000002	29.75	19.975	36.65
46	18.475	24.175	34.475	22.875
47	26.275	16.775000000000002	18.875	38.074999999999996
48	22.15	23.1	29.25	25.5
49	32.0	17.1	28.575	22.325
50	16.475	16.6	25.25	41.675000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	1.0
25	0.5
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	1.0
32	1.0
33	1.5
34	2.0
35	2.5
36	3.0
37	14.5
38	26.0
39	38.5
40	51.0
41	112.0
42	173.0
43	158.5
44	144.0
45	173.5
46	203.0
47	203.5
48	204.0
49	415.0
50	626.0
51	615.0
52	604.0
53	434.0
54	264.0
55	376.5
56	489.0
57	428.0
58	367.0
59	431.5
60	496.0
61	330.5
62	165.0
63	118.5
64	72.0
65	66.5
66	61.0
67	37.5
68	14.0
69	14.5
70	15.0
71	16.5
72	18.0
73	9.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80811808118082	24.325
2	10.922509225092252	7.3999999999999995
3	5.0922509225092245	5.175
4	2.5830258302583027	3.5000000000000004
5	2.1402214022140225	3.6249999999999996
6	0.959409594095941	1.95
7	1.2546125461254614	2.9749999999999996
8	0.959409594095941	2.6
9	0.22140221402214022	0.675
>10	3.394833948339483	24.75
>50	0.5904059040590406	14.424999999999999
>100	0.07380073800738007	8.6
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	344	8.6	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	86	2.15	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	84	2.1	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	78	1.95	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	74	1.8499999999999999	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	70	1.7500000000000002	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	66	1.6500000000000001	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	64	1.6	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	55	1.375	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	47	1.175	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	42	1.05	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	41	1.0250000000000001	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	40	1.0	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	37	0.9249999999999999	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	37	0.9249999999999999	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	37	0.9249999999999999	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	36	0.8999999999999999	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	36	0.8999999999999999	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTC	35	0.8750000000000001	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	34	0.8500000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	32	0.8	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	27	0.675	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	26	0.65	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	23	0.575	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	22	0.5499999999999999	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	22	0.5499999999999999	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	21	0.525	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	21	0.525	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	20	0.5	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	18	0.44999999999999996	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	18	0.44999999999999996	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	17	0.42500000000000004	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	17	0.42500000000000004	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	16	0.4	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	16	0.4	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	16	0.4	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	16	0.4	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	15	0.375	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	15	0.375	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	14	0.35000000000000003	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	13	0.325	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	13	0.325	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	13	0.325	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	13	0.325	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	12	0.3	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATAT	12	0.3	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	12	0.3	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	12	0.3	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	12	0.3	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	11	0.27499999999999997	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	11	0.27499999999999997	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	11	0.27499999999999997	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	11	0.27499999999999997	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	10	0.25	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	10	0.25	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	9	0.22499999999999998	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	9	0.22499999999999998	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	9	0.22499999999999998	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	8	0.2	No Hit
GATATTTGTCCTCATGGAAGGACGTAGCCTATAAATGCTGTGGCTATGAC	8	0.2	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	8	0.2	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	8	0.2	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	8	0.2	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	8	0.2	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	8	0.2	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	8	0.2	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	8	0.2	No Hit
ATTTCGTCTTCTGAGATGGAGTCCTTGCGACTTGTGCTTCGAAGGCGAAC	8	0.2	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	8	0.2	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	8	0.2	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	8	0.2	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	7	0.17500000000000002	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	7	0.17500000000000002	No Hit
GTCCATGGTGTACACTTTCACTCCCCGGAAGCTGAAGCCCGAACACCGAT	7	0.17500000000000002	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	7	0.17500000000000002	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	7	0.17500000000000002	No Hit
TTGGCGCGGCACGTGTAGCTCAGGTTCCTCCGCACGCTGCGCTTGAAGAA	7	0.17500000000000002	No Hit
AGGATCGAGACGGCTTGTGATTTCTCTCACGAGACAGAGACCTTTCTCTT	7	0.17500000000000002	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	7	0.17500000000000002	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	7	0.17500000000000002	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	7	0.17500000000000002	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	7	0.17500000000000002	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	7	0.17500000000000002	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	7	0.17500000000000002	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	7	0.17500000000000002	No Hit
CTGTGTTCTCCCTACAACTGTGTGTGTGATCCTCCTCAATGCTTCCCTCT	7	0.17500000000000002	No Hit
CTTGCGGATGCCATCTTCTTCCACTTCGTAGTAGTCAGGCCCACAGGCTC	6	0.15	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	6	0.15	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
TGGAGGAGGAACAGTGGGATATGGCCCTGGAGATCGATATGGCATGTTAG	6	0.15	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	6	0.15	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	6	0.15	No Hit
GACACACTTGTACCTCCTGCTGAGACTCCAAATATAGTGACCCTGTCACG	6	0.15	No Hit
AGAAAGGTCAGAGAAGGGATGATATTCACTAGGTCAGCAGCAAATCCTTC	6	0.15	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	6	0.15	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
CAGGTATGTCCTCAGGTCCTGGTCTATATGCTCAAACACTAGGGTGACCT	6	0.15	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	6	0.15	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	6	0.15	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	5	0.125	No Hit
AGGCCAGTCGGAGTGCATGGACCTTTTGCATTTCCTCACCGGAGAAGCTA	5	0.125	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGT	5	0.125	No Hit
GCAGACGCAACTCTCGGTAGGTCCTTTTGGCGTGAATGATGGACTGAAAC	5	0.125	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	5	0.125	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	5	0.125	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	5	0.125	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	5	0.125	No Hit
AGCACAAAGTCCTCCCGGTACTCCTGGCGGATATCTTTGCGCAGTTTGGC	5	0.125	No Hit
ACAGAACCCACAAGCATTCTGTAGTCCTTGTGAGTAAGAGGAGACAGCGG	5	0.125	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	5	0.125	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	5	0.125	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTC	5	0.125	No Hit
TATGCACTCCATGACATGTATCTGTAGAGTGTCCATATCAGGAGCCTGAT	5	0.125	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	5	0.125	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	5	0.125	No Hit
GTGGAGACTCCTCACAATGCACCGCCTCGAATTGCTCTTGCCCTTCCTAC	5	0.125	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	5	0.125	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
ATGGCCATGATGGCATTGCGGATGTCCTCCTCATCAGCGTTGGAGCTTAC	5	0.125	No Hit
CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTCAGTACATATCTCGTA	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	5	0.125	No Hit
CCAGGTGAACGAGAGCCATGATGGTTGTGTAGCTATGGAACAGGTCCTCT	5	0.125	No Hit
CTCCTTCTTCGAGACTTTCACAATTTTTATGCTGCTGCCCGAAGTCTTCT	5	0.125	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	5	0.125	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	5	0.125	No Hit
CGTTGGTCACAAGGAGATTGCCTTTCTTGCTCACACTGCTGACGTACTCA	5	0.125	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCC	20	6.952051E-4	44.000004	29
GCCCACT	20	6.952051E-4	44.000004	33
GCGATGC	20	6.952051E-4	44.000004	41
GATGCAT	20	6.952051E-4	44.000004	43
GGCGATG	20	6.952051E-4	44.000004	40
GGTTTGC	20	6.952051E-4	44.000004	28
CCCACTG	20	6.952051E-4	44.000004	34
CATCAGG	20	6.952051E-4	44.000004	23
CTGGCGA	20	6.952051E-4	44.000004	38
TGCCCAC	20	6.952051E-4	44.000004	32
TTCTCCA	20	6.952051E-4	44.000004	18
CCATCAG	20	6.952051E-4	44.000004	22
TCCATCA	20	6.952051E-4	44.000004	21
AGGTTTG	20	6.952051E-4	44.000004	27
ATGCATG	20	6.952051E-4	44.000004	44
TCTCCAT	20	6.952051E-4	44.000004	19
TGGCGAT	20	6.952051E-4	44.000004	39
CGATGCA	20	6.952051E-4	44.000004	42
ACTGGCG	20	6.952051E-4	44.000004	37
GTAATAC	20	6.952051E-4	44.000004	24
>>END_MODULE
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143252 spots for SRR25943861.sra
Written 143252 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
Read 143242 spots for SRR25943861.sra
Written 143242 spots for SRR25943861.sra
SRR ids: ['SRR25943861.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_05uybsxa
SRR25943861.sra spots: 2291882
blocks: [[1, 143242], [143243, 286484], [286485, 429726], [429727, 572968], [572969, 716210], [716211, 859452], [859453, 1002694], [1002695, 1145936], [1145937, 1289178], [1289179, 1432420], [1432421, 1575662], [1575663, 1718904], [1718905, 1862146], [1862147, 2005388], [2005389, 2148630], [2148631, 2291882]]
SRR25943861 file size 1
SRR25943861 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25943861 SRR25943861_1.fastq
Input file:	SRR25943861_1.fastq
trimmed:	SRR25943861-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Tue May  5 08:29:50 2026 >> started

Tue May  5 08:29:51 2026 >> done (1.555s)
2291882 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2291882 (100.00%) reads available; of these:
     47 ( 0.00%) trimmed reads available after processing
2291835 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 46	     15	  0.00%
 47	      2	  0.00%
 48	      9	  0.00%
 49	     21	  0.00%
 50	2291835	100.00%
2291882 reads passed initial QC


criterion=sequence-density
sequence-density=9.19
sequence-density-rank=1
fanout-score=1.39
fanout-score-rank=8
prefix-density=0.07
prefix-fanout=1.4
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGT


criterion=fanout-score
sequence-density=0.47
sequence-density-rank=35
fanout-score=25.93
fanout-score-rank=1
prefix-density=12.09
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 08:30:10
                             Started mapping on |	May 05 08:30:11
                                    Finished on |	May 05 08:30:21
       Mapping speed, Million of reads per hour |	825.08

                          Number of input reads |	2291882
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1625031
                        Uniquely mapped reads % |	70.90%
                          Average mapped length |	49.59
                       Number of splices: Total |	1429
            Number of splices: Annotated (sjdb) |	838
                       Number of splices: GT/AG |	1312
                       Number of splices: GC/AG |	15
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	101
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.09%
                        Deletion average length |	1.14
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	257046
             % of reads mapped to multiple loci |	11.22%
        Number of reads mapped to too many loci |	268644
             % of reads mapped to too many loci |	11.72%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.16%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	409805	409805	409805
N_multimapping	257046	257046	257046
N_noFeature	6847	1565144	6875
N_ambiguous	74749	34	15585
UnstrandedReadsAssigned:1543435 PositiveStrandReadsAssigned:59853 NegativeStrandReadsAssigned:1602571
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943861 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943861-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,291,882 reads, 2,047,631 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,119 rounds

 109283 SRR25943861.ke.tsv
  52636 SRR25943861.se.tsv
 161919 total
==> SRR25943861.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943861.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943861 completed mapping pipeline successfully
