Starting /dee2/code/volunteer_pipeline.sh SRR25943850
    current disk space = 1266260620
    free memory = 195635652 
SRR25943850 SRAfilesize
f637822711624f5b40c9b6032f8dbc05  SRR25943850.sra
SRR25943850.sra file validated
SRR25943850 is single end
SRR25943850 is conventional basespace
SRR25943850 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943850_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362	32.0	32.0	32.0	32.0	32.0
2	31.3755	32.0	32.0	32.0	32.0	32.0
3	31.5095	32.0	32.0	32.0	32.0	32.0
4	31.506	32.0	32.0	32.0	32.0	32.0
5	31.59825	32.0	32.0	32.0	32.0	32.0
6	35.068	36.0	36.0	36.0	36.0	36.0
7	35.06275	36.0	36.0	36.0	36.0	36.0
8	34.8825	36.0	36.0	36.0	32.0	36.0
9	34.90375	36.0	36.0	36.0	32.0	36.0
10	34.91075	36.0	36.0	36.0	32.0	36.0
11	34.9005	36.0	36.0	36.0	36.0	36.0
12	35.052	36.0	36.0	36.0	36.0	36.0
13	35.03075	36.0	36.0	36.0	36.0	36.0
14	34.91175	36.0	36.0	36.0	32.0	36.0
15	34.86575	36.0	36.0	36.0	32.0	36.0
16	34.972	36.0	36.0	36.0	36.0	36.0
17	34.952	36.0	36.0	36.0	32.0	36.0
18	34.88375	36.0	36.0	36.0	36.0	36.0
19	34.97925	36.0	36.0	36.0	36.0	36.0
20	34.944	36.0	36.0	36.0	36.0	36.0
21	34.965	36.0	36.0	36.0	36.0	36.0
22	34.888	36.0	36.0	36.0	32.0	36.0
23	35.03875	36.0	36.0	36.0	36.0	36.0
24	34.75975	36.0	36.0	36.0	32.0	36.0
25	35.00075	36.0	36.0	36.0	36.0	36.0
26	34.77475	36.0	36.0	36.0	32.0	36.0
27	34.83125	36.0	36.0	36.0	32.0	36.0
28	34.6135	36.0	36.0	36.0	32.0	36.0
29	34.77225	36.0	36.0	36.0	32.0	36.0
30	34.86175	36.0	36.0	36.0	32.0	36.0
31	35.019	36.0	36.0	36.0	36.0	36.0
32	34.71925	36.0	36.0	36.0	32.0	36.0
33	34.69925	36.0	36.0	36.0	32.0	36.0
34	34.70475	36.0	36.0	36.0	32.0	36.0
35	34.905	36.0	36.0	36.0	36.0	36.0
36	34.83525	36.0	36.0	36.0	32.0	36.0
37	34.8165	36.0	36.0	36.0	32.0	36.0
38	34.79225	36.0	36.0	36.0	32.0	36.0
39	34.978	36.0	36.0	36.0	36.0	36.0
40	34.46525	36.0	36.0	36.0	32.0	36.0
41	34.73975	36.0	36.0	36.0	32.0	36.0
42	34.69	36.0	36.0	36.0	32.0	36.0
43	34.4985	36.0	36.0	36.0	32.0	36.0
44	34.57	36.0	36.0	36.0	32.0	36.0
45	34.51575	36.0	36.0	36.0	32.0	36.0
46	34.285	36.0	36.0	36.0	32.0	36.0
47	34.578	36.0	36.0	36.0	32.0	36.0
48	34.25	36.0	36.0	36.0	32.0	36.0
49	34.6055	36.0	36.0	36.0	32.0	36.0
50	34.08775	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	1.0
25	10.0
26	11.0
27	40.0
28	45.0
29	73.0
30	103.0
31	139.0
32	184.0
33	291.0
34	631.0
35	2471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.050000000000004	18.075	24.775	29.099999999999998
2	26.900000000000002	17.474999999999998	28.349999999999998	27.275
3	35.225	23.175	21.5	20.1
4	30.475	22.425	21.975	25.124999999999996
5	16.150000000000002	27.750000000000004	28.000000000000004	28.1
6	31.698774080560423	25.769326995246434	24.86865148861646	17.663247435576682
7	31.974999999999998	16.650000000000002	31.825	19.55
8	23.724999999999998	19.425	18.35	38.5
9	20.549999999999997	18.525	30.525000000000002	30.4
10	25.825	15.25	28.025	30.9
11	26.35	33.324999999999996	15.75	24.575
12	37.824999999999996	19.7	20.3	22.175
13	35.8	28.375	17.150000000000002	18.675
14	19.5	20.549999999999997	25.825	34.125
15	17.075000000000003	32.425	20.549999999999997	29.95
16	15.425	31.75	28.025	24.8
17	22.5	21.025	26.224999999999998	30.25
18	21.224999999999998	14.774999999999999	41.15	22.85
19	22.400000000000002	24.05	30.175	23.375
20	29.95	14.899999999999999	25.825	29.325000000000003
21	17.224999999999998	20.1	34.4	28.275
22	19.55	17.825	33.800000000000004	28.825
23	30.525000000000002	16.625	23.1	29.75
24	27.474999999999998	16.45	25.5	30.575000000000003
25	1.075	4.075	83.975	10.875
26	15.275	23.674999999999997	19.475	41.575
27	29.2	25.974999999999998	21.9	22.925
28	15.950000000000001	22.45	30.9	30.7
29	34.050000000000004	24.575	15.125	26.25
30	27.150000000000002	21.0	24.2	27.650000000000002
31	32.375	23.150000000000002	17.075000000000003	27.400000000000002
32	26.8	19.55	20.9	32.75
33	30.425	22.8	15.950000000000001	30.825000000000003
34	22.675	18.45	35.05	23.825
35	26.474999999999998	17.175	30.45	25.900000000000002
36	31.825	10.6	29.9	27.675
37	22.45	31.574999999999996	24.85	21.125
38	32.6	12.225	20.95	34.225
39	32.525	25.1	18.25	24.125
40	21.375	26.5	21.0	31.125000000000004
41	21.224999999999998	28.799999999999997	20.025000000000002	29.95
42	32.125	17.95	27.05	22.875
43	27.325	22.45	21.85	28.375
44	28.375	17.925	23.599999999999998	30.099999999999998
45	13.65	29.599999999999998	19.2	37.55
46	20.25	27.85	32.35	19.55
47	28.249999999999996	15.8	15.125	40.825
48	21.860930465232617	25.312656328164078	29.689844922461226	23.13656828414207
49	32.675	18.75	25.775	22.8
50	16.525000000000002	22.05	22.225	39.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	2.0
30	4.0
31	3.0
32	2.0
33	1.5
34	1.0
35	4.5
36	8.0
37	19.0
38	30.0
39	37.5
40	45.0
41	106.5
42	168.0
43	204.0
44	240.0
45	246.5
46	253.0
47	215.0
48	177.0
49	422.5
50	668.0
51	611.5
52	555.0
53	417.5
54	280.0
55	416.5
56	553.0
57	444.0
58	335.0
59	370.5
60	406.0
61	280.5
62	155.0
63	107.0
64	59.0
65	48.0
66	37.0
67	23.5
68	10.0
69	10.5
70	11.0
71	6.5
72	2.0
73	1.5
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.05
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17073170731707	23.25
2	10.54287962234461	6.7
3	5.035405192761605	4.8
4	2.1243115656963023	2.7
5	2.2029897718332023	3.5000000000000004
6	1.1801730920535012	2.25
7	0.6294256490952006	1.4000000000000001
8	0.3147128245476003	0.8
9	0.3147128245476003	0.8999999999999999
>10	3.6978756884343036	25.124999999999996
>50	0.3933910306845004	8.05
>100	0.3933910306845004	20.525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	295	7.375	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	162	4.05	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	148	3.6999999999999997	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	109	2.725	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	107	2.675	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	74	1.8499999999999999	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	69	1.725	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	64	1.6	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	59	1.4749999999999999	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	56	1.4000000000000001	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	48	1.2	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	41	1.0250000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	39	0.975	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	35	0.8750000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	33	0.8250000000000001	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	33	0.8250000000000001	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	32	0.8	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	32	0.8	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	30	0.75	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	30	0.75	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	28	0.7000000000000001	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	28	0.7000000000000001	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	27	0.675	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	26	0.65	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	26	0.65	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	25	0.625	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	25	0.625	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	25	0.625	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	23	0.575	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	21	0.525	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	21	0.525	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	20	0.5	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	20	0.5	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	20	0.5	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	20	0.5	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	19	0.475	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	19	0.475	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	18	0.44999999999999996	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	17	0.42500000000000004	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	16	0.4	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	16	0.4	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	15	0.375	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	14	0.35000000000000003	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	14	0.35000000000000003	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	14	0.35000000000000003	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	14	0.35000000000000003	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	13	0.325	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAAT	13	0.325	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	13	0.325	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	11	0.27499999999999997	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	11	0.27499999999999997	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	10	0.25	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	10	0.25	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	10	0.25	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	10	0.25	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	10	0.25	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	10	0.25	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	9	0.22499999999999998	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	9	0.22499999999999998	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	9	0.22499999999999998	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	9	0.22499999999999998	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	8	0.2	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	8	0.2	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	8	0.2	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	8	0.2	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	7	0.17500000000000002	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	7	0.17500000000000002	No Hit
GTCCATGGTGTACACTTTCACTCCCCGGAAGCTGAAGCCCGAACACCGAT	7	0.17500000000000002	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	7	0.17500000000000002	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	7	0.17500000000000002	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	7	0.17500000000000002	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	7	0.17500000000000002	No Hit
AGGCCAGTCGGAGTGCATGGACCTTTTGCATTTCCTCACCGGAGAAGCTA	6	0.15	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
TCAGCGTTCACTAGGCAGACCATCTGCAACACAATGGTGGCTCCCATACG	6	0.15	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	6	0.15	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	6	0.15	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	6	0.15	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	6	0.15	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	6	0.15	No Hit
AAACGCCCACTTTGACATAGACTTTATAGACCATACCACCAATACCGAAA	6	0.15	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	6	0.15	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	6	0.15	No Hit
TCTCTGGTCCAGCTGTGGTGGAGCTGGTGAAGGTGAGCAAGCAGAGCGCC	6	0.15	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	6	0.15	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	6	0.15	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	6	0.15	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	5	0.125	No Hit
TCTGTTCCTCAGGCACCTGGACTTTCCTTGCCTTCTTCATGATTGGTTGA	5	0.125	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	5	0.125	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	5	0.125	No Hit
TGGTCAGCCTCCTGGATGGTCCTGTCCGCAATACTGCCCAGCTGCTCATC	5	0.125	No Hit
TTGGCAGGCTTGGTTTCCTCGATCTCAGAAGACAGCCATCGGTAGGTACG	5	0.125	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	5	0.125	No Hit
TTATGAAAGGCAAGAAGTCACTTCCATACATGGAGAAGCCATCAGGGTGC	5	0.125	No Hit
TCCATTGGTGGTGAAGATGGCAGTTCCATTTCCATATGGGTTGTCATTTA	5	0.125	No Hit
CTGGCTGTCCAATCCACCGACTATTAACACATGTCACAGTGTCTGAGCCA	5	0.125	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	5	0.125	No Hit
GAACGCCTTTACCAAACGGAGTGTTTCTTCCACACTGCGGCCCACCGGAA	5	0.125	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	5	0.125	No Hit
AGCTCGTCCTCTGTGGTCTTCTGGTAGACTGGGTTGTCAAAGTTTATGCT	5	0.125	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	5	0.125	No Hit
CTCCACACAGGTGTCCCTGGCTGATGTGCAGAGTCACCTGCCATGGCCAC	5	0.125	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	5	0.125	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	5	0.125	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	5	0.125	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	5	0.125	No Hit
CCAGCTGCCCTTGAGTGATGTACTTTTTCCACCAGAGGTAGGGACGCATG	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	5	0.125	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	5	0.125	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	5	0.125	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	5	0.125	No Hit
CAGGTACAAGGCAAGAGGATTTGCCCCAGCAAGCCAACCATTCTCTGAAC	5	0.125	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTGCCCACTGGCGATGCATGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGTCC	20	6.952051E-4	44.000004	44
GTAATAC	20	6.952051E-4	44.000004	24
ATCTGTC	25	0.002084305	35.2	43
GGAGGCT	40	5.87456E-4	27.500002	3
CGGGCCT	45	0.0011696975	24.444447	28
GGCCTGG	45	0.0011696975	24.444447	30
GCCTGGA	45	0.0011696975	24.444447	31
TCGGGCC	45	0.0011696975	24.444447	27
CTTCATT	50	0.0021617375	22.0	19
TTCATTC	50	0.0021617375	22.0	20
GCGATCT	50	0.0021617375	22.0	42
CATTCTT	50	0.0021617375	22.0	15
GCAGCGA	50	0.0021617375	22.0	39
TTCTCGG	50	0.0021617375	22.0	24
TTCTTCA	50	0.0021617375	22.0	17
GATCTGT	50	0.0021617375	22.0	44
CCATTCT	50	0.0021617375	22.0	14
GGCCATT	50	0.0021617375	22.0	12
AGGCCAT	50	0.0021617375	22.0	11
AGGCTCA	50	0.0021617375	22.0	5
>>END_MODULE
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480641 spots for SRR25943850.sra
Written 480641 spots for SRR25943850.sra
Read 480648 spots for SRR25943850.sra
Written 480648 spots for SRR25943850.sra
SRR ids: ['SRR25943850.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tsui_g2t
SRR25943850.sra spots: 3845135
blocks: [[1, 480641], [480642, 961282], [961283, 1441923], [1441924, 1922564], [1922565, 2403205], [2403206, 2883846], [2883847, 3364487], [3364488, 3845135]]
SRR25943850 file size 664288
SRR25943850 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943850 SRR25943850_1.fastq
Input file:	SRR25943850_1.fastq
trimmed:	SRR25943850-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:38:01 2026 >> started

Tue May  5 05:38:02 2026 >> done (1.170s)
3845135 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3845135 (100.00%) reads available; of these:
     78 ( 0.00%) trimmed reads available after processing
3845057 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      2	  0.00%
 46	     14	  0.00%
 47	      4	  0.00%
 48	     23	  0.00%
 49	     34	  0.00%
 50	3845057	100.00%
3845135 reads passed initial QC


criterion=sequence-density
sequence-density=8.03
sequence-density-rank=1
fanout-score=1.28
fanout-score-rank=7
prefix-density=0.06
prefix-fanout=1.3
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTG


criterion=fanout-score
sequence-density=0.56
sequence-density-rank=31
fanout-score=20.15
fanout-score-rank=1
prefix-density=11.21
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:38:08
                             Started mapping on |	May 05 05:38:08
                                    Finished on |	May 05 05:38:15
       Mapping speed, Million of reads per hour |	1977.50

                          Number of input reads |	3845135
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2805940
                        Uniquely mapped reads % |	72.97%
                          Average mapped length |	49.55
                       Number of splices: Total |	1838
            Number of splices: Annotated (sjdb) |	700
                       Number of splices: GT/AG |	1655
                       Number of splices: GC/AG |	38
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	145
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.08
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	438618
             % of reads mapped to multiple loci |	11.41%
        Number of reads mapped to too many loci |	489190
             % of reads mapped to too many loci |	12.72%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.89%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	600577	600577	600577
N_multimapping	438618	438618	438618
N_noFeature	3632	2722057	3642
N_ambiguous	106173	31	23262
UnstrandedReadsAssigned:2696135 PositiveStrandReadsAssigned:83852 NegativeStrandReadsAssigned:2779036
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943850 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943850-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,845,135 reads, 3,580,419 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,148 rounds

 109283 SRR25943850.ke.tsv
  52636 SRR25943850.se.tsv
 161919 total
==> SRR25943850.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943850.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943850 completed mapping pipeline successfully
