Starting /dee2/code/volunteer_pipeline.sh SRR25943854
    current disk space = 489465125888
    free memory = 306978244 
SRR25943854 SRAfilesize
7702e2e92b76e689482436991a172252  SRR25943854.sra
SRR25943854.sra file validated
SRR25943854 is single end
SRR25943854 is conventional basespace
SRR25943854 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224	32.0	32.0	32.0	32.0	32.0
2	31.2485	32.0	32.0	32.0	32.0	32.0
3	31.53375	32.0	32.0	32.0	32.0	32.0
4	31.425	32.0	32.0	32.0	32.0	32.0
5	31.54275	32.0	32.0	32.0	32.0	32.0
6	35.033	36.0	36.0	36.0	36.0	36.0
7	34.9945	36.0	36.0	36.0	36.0	36.0
8	34.89925	36.0	36.0	36.0	32.0	36.0
9	34.907	36.0	36.0	36.0	32.0	36.0
10	34.92125	36.0	36.0	36.0	32.0	36.0
11	34.96325	36.0	36.0	36.0	36.0	36.0
12	35.00425	36.0	36.0	36.0	36.0	36.0
13	35.052	36.0	36.0	36.0	36.0	36.0
14	34.879	36.0	36.0	36.0	32.0	36.0
15	34.67225	36.0	36.0	36.0	32.0	36.0
16	34.91775	36.0	36.0	36.0	32.0	36.0
17	34.802	36.0	36.0	36.0	32.0	36.0
18	34.81675	36.0	36.0	36.0	32.0	36.0
19	34.99	36.0	36.0	36.0	36.0	36.0
20	34.822	36.0	36.0	36.0	32.0	36.0
21	35.05425	36.0	36.0	36.0	36.0	36.0
22	34.91025	36.0	36.0	36.0	36.0	36.0
23	34.92	36.0	36.0	36.0	36.0	36.0
24	34.87525	36.0	36.0	36.0	36.0	36.0
25	34.79125	36.0	36.0	36.0	36.0	36.0
26	34.647	36.0	36.0	36.0	32.0	36.0
27	34.871	36.0	36.0	36.0	32.0	36.0
28	34.675	36.0	36.0	36.0	32.0	36.0
29	34.77275	36.0	36.0	36.0	32.0	36.0
30	34.72775	36.0	36.0	36.0	32.0	36.0
31	34.76	36.0	36.0	36.0	32.0	36.0
32	34.68225	36.0	36.0	36.0	32.0	36.0
33	34.7665	36.0	36.0	36.0	32.0	36.0
34	34.75525	36.0	36.0	36.0	32.0	36.0
35	34.84725	36.0	36.0	36.0	32.0	36.0
36	34.85575	36.0	36.0	36.0	32.0	36.0
37	34.842	36.0	36.0	36.0	32.0	36.0
38	34.68175	36.0	36.0	36.0	32.0	36.0
39	34.78025	36.0	36.0	36.0	32.0	36.0
40	34.46	36.0	36.0	36.0	32.0	36.0
41	34.646	36.0	36.0	36.0	32.0	36.0
42	34.6275	36.0	36.0	36.0	32.0	36.0
43	34.36125	36.0	36.0	36.0	32.0	36.0
44	34.60575	36.0	36.0	36.0	32.0	36.0
45	34.49925	36.0	36.0	36.0	32.0	36.0
46	34.338	36.0	36.0	36.0	32.0	36.0
47	34.379	36.0	36.0	36.0	32.0	36.0
48	33.97875	36.0	36.0	36.0	32.0	36.0
49	34.3095	36.0	36.0	36.0	32.0	36.0
50	33.978	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	4.0
25	11.0
26	15.0
27	32.0
28	46.0
29	84.0
30	115.0
31	124.0
32	212.0
33	294.0
34	666.0
35	2395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.025	16.125	24.325	33.525
2	25.2	15.049999999999999	34.25	25.5
3	38.725	23.7	19.2	18.375
4	37.15	21.0	18.825	23.025000000000002
5	17.175	33.4	24.349999999999998	25.074999999999996
6	38.819409704852426	20.185092546273136	20.335167583791897	20.66033016508254
7	36.825	16.225	28.775000000000002	18.175
8	17.974999999999998	17.599999999999998	17.525	46.9
9	19.275000000000002	18.55	34.599999999999994	27.575
10	24.85	15.024999999999999	23.325000000000003	36.8
11	26.525	32.824999999999996	15.725	24.925
12	37.775	16.525000000000002	18.725	26.974999999999998
13	37.9	23.05	17.825	21.224999999999998
14	19.925	18.7	22.6	38.775
15	16.400000000000002	26.424999999999997	22.35	34.825
16	15.775	34.75	26.275	23.200000000000003
17	18.05	19.675	31.825	30.45
18	18.775	13.950000000000001	46.5	20.775
19	19.025	22.275	27.700000000000003	31.0
20	23.45	13.0	30.599999999999998	32.95
21	14.924999999999999	21.925	37.375	25.775
22	14.299999999999999	16.5	34.9	34.300000000000004
23	26.5	22.325	22.15	29.025000000000002
24	22.625	12.55	29.425	35.4
25	1.425	4.475	79.65	14.45
26	14.524999999999999	18.525	16.475	50.475
27	28.725	21.0	29.675	20.599999999999998
28	17.549999999999997	20.375	23.799999999999997	38.275
29	40.849999999999994	22.525000000000002	12.125	24.5
30	33.575	20.075000000000003	19.825	26.525
31	35.675000000000004	20.05	14.799999999999999	29.475
32	19.325	18.15	23.849999999999998	38.675
33	28.575	20.7	16.775000000000002	33.95
34	24.875	15.6	37.125	22.400000000000002
35	30.65	20.775	23.025000000000002	25.55
36	38.75	12.425	23.05	25.775
37	22.575	38.675	20.875	17.875
38	38.3	10.65	18.725	32.324999999999996
39	37.1	22.325	15.7	24.875
40	21.975	23.799999999999997	18.7	35.525
41	19.825	33.575	15.1	31.5
42	39.975	17.4	19.8	22.825
43	25.124999999999996	22.45	19.7	32.725
44	34.675	14.674999999999999	21.975	28.675
45	12.275	34.575	15.8	37.35
46	18.65	23.474999999999998	39.324999999999996	18.55
47	26.224999999999998	14.075	13.525	46.175
48	18.975	23.575	35.825	21.625
49	34.475	17.474999999999998	25.474999999999998	22.575
50	17.2	15.675	27.650000000000002	39.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	1.0
32	1.0
33	1.0
34	1.0
35	3.0
36	5.0
37	10.0
38	15.0
39	29.5
40	44.0
41	86.5
42	129.0
43	128.5
44	128.0
45	161.0
46	194.0
47	176.5
48	159.0
49	348.0
50	537.0
51	507.5
52	478.0
53	370.0
54	262.0
55	370.0
56	478.0
57	438.0
58	398.0
59	553.5
60	709.0
61	485.0
62	261.0
63	176.5
64	92.0
65	69.5
66	47.0
67	32.0
68	17.0
69	22.5
70	28.0
71	22.0
72	16.0
73	8.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65506329113924	23.275000000000002
2	10.680379746835444	6.75
3	4.90506329113924	4.65
4	2.7689873417721516	3.5000000000000004
5	1.1867088607594938	1.875
6	1.1075949367088607	2.1
7	1.0284810126582278	2.275
8	0.39556962025316456	1.0
9	0.5537974683544303	1.575
>10	3.0063291139240507	21.25
>50	0.5537974683544303	13.900000000000002
>100	0.07911392405063292	3.925
>500	0.07911392405063292	13.925
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	557	13.925	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	157	3.925	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	98	2.45	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	88	2.1999999999999997	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	86	2.15	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	78	1.95	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	76	1.9	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	67	1.675	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	63	1.575	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	40	1.0	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	38	0.95	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	36	0.8999999999999999	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	35	0.8750000000000001	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	32	0.8	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	31	0.775	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	31	0.775	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	31	0.775	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	30	0.75	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	30	0.75	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	28	0.7000000000000001	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	27	0.675	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	27	0.675	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	26	0.65	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	26	0.65	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	26	0.65	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	23	0.575	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	21	0.525	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	21	0.525	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	21	0.525	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	19	0.475	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACACCTGAGGCAT	19	0.475	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	19	0.475	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	19	0.475	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	17	0.42500000000000004	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	17	0.42500000000000004	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	16	0.4	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	15	0.375	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	15	0.375	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	15	0.375	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	14	0.35000000000000003	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	14	0.35000000000000003	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	14	0.35000000000000003	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	13	0.325	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	12	0.3	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	11	0.27499999999999997	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	11	0.27499999999999997	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	10	0.25	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	9	0.22499999999999998	No Hit
AGGCCAGTCGGAGTGCATGGACCTTTTGCATTTCCTCACCGGAGAAGCTA	9	0.22499999999999998	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	9	0.22499999999999998	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	9	0.22499999999999998	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	9	0.22499999999999998	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	9	0.22499999999999998	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	8	0.2	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	8	0.2	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	8	0.2	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	8	0.2	No Hit
GGTAGCAAAGAGAGTCAATAACCCTTTCAGGAACGAGACTATTTCATTTC	8	0.2	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	7	0.17500000000000002	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	7	0.17500000000000002	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	7	0.17500000000000002	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	7	0.17500000000000002	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACACCTGAGGCATCTC	7	0.17500000000000002	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	7	0.17500000000000002	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	7	0.17500000000000002	No Hit
TTGAAAGCAACCCAACAGGACCGGTCACACACTCTCCACCAAGTTTAAAA	7	0.17500000000000002	No Hit
AATCGGACCTCTGCCTCTTTGTCTTCGACGCCGTTGGCCACCATCTTGGC	7	0.17500000000000002	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	7	0.17500000000000002	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	7	0.17500000000000002	No Hit
CAGGTACAAGGCAAGAGGATTTGCCCCAGCAAGCCAACCATTCTCTGAAC	7	0.17500000000000002	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	6	0.15	No Hit
TGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTC	6	0.15	No Hit
CAGCTGCTGCCCGCCGACATGTCTCCTGCACGCCGCCCACGACCCTCCAG	6	0.15	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	6	0.15	No Hit
CATCAGAAGACTCGGACACCAGCTTCCCATCTCGGGTTTCAATCTTCTTC	6	0.15	No Hit
TTCTTCTCAAGCAGCTCACGAATCTGCTCCTTTAGGACCTCCACCTCCTC	6	0.15	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	6	0.15	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	6	0.15	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACACCTGAGGCATCTC	6	0.15	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	6	0.15	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	6	0.15	No Hit
CGGCGCCAATTCCTTTCACCGAGTTCGCGAGGCAAGACGCGGAGACTCGC	6	0.15	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	6	0.15	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	6	0.15	No Hit
GTCCATGGTGTACACTTTCACTCCCCGGAAGCTGAAGCCCGAACACCGAT	5	0.125	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	5	0.125	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	5	0.125	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	5	0.125	No Hit
TTATGAAAGGCAAGAAGTCACTTCCATACATGGAGAAGCCATCAGGGTGC	5	0.125	No Hit
TAGGAGTGCATGTCTCCAAAGTCCTTCTCGAAGAAGACGTAGGCCTTGCT	5	0.125	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	5	0.125	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	5	0.125	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	5	0.125	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	5	0.125	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	5	0.125	No Hit
CTTGGAGAGATTGAAATAGGTCTCTTTGATATCAAAGTCCTTGTGGATGA	5	0.125	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	5	0.125	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	5	0.125	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	20	6.952051E-4	44.000004	6
TCCTTTC	20	6.952051E-4	44.000004	38
GAGGTAT	20	6.952051E-4	44.000004	1
TGTTGAA	20	6.952051E-4	44.000004	6
GAGAAGT	20	6.952051E-4	44.000004	12
GTTGAAA	20	6.952051E-4	44.000004	7
CCGGAGA	20	6.952051E-4	44.000004	9
TGGCAAC	20	6.952051E-4	44.000004	25
GCCTTAC	20	6.952051E-4	44.000004	32
CGGAGAA	20	6.952051E-4	44.000004	10
ACATGTG	20	6.952051E-4	44.000004	16
CACACAG	20	6.952051E-4	44.000004	14
AGAAGTT	20	6.952051E-4	44.000004	13
GAAACAC	20	6.952051E-4	44.000004	10
CCTGTTG	20	6.952051E-4	44.000004	4
AACGAGG	20	6.952051E-4	44.000004	29
TCCTAGG	20	6.952051E-4	44.000004	7
GTACTTG	20	6.952051E-4	44.000004	2
TGGTAAT	20	6.952051E-4	44.000004	22
ACTTGCC	20	6.952051E-4	44.000004	4
>>END_MODULE
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210176 spots for SRR25943854.sra
Written 210176 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
Read 210173 spots for SRR25943854.sra
Written 210173 spots for SRR25943854.sra
SRR ids: ['SRR25943854.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_zp9nx9p1
SRR25943854.sra spots: 3362771
blocks: [[1, 210173], [210174, 420346], [420347, 630519], [630520, 840692], [840693, 1050865], [1050866, 1261038], [1261039, 1471211], [1471212, 1681384], [1681385, 1891557], [1891558, 2101730], [2101731, 2311903], [2311904, 2522076], [2522077, 2732249], [2732250, 2942422], [2942423, 3152595], [3152596, 3362771]]
SRR25943854 file size 137187
SRR25943854 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25943854 SRR25943854_1.fastq
Input file:	SRR25943854_1.fastq
trimmed:	SRR25943854-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Tue May  5 08:26:42 2026 >> started

Tue May  5 08:26:44 2026 >> done (1.707s)
3362771 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3362771 (100.00%) reads available; of these:
     67 ( 0.00%) trimmed reads available after processing
3362704 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      1	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	     14	  0.00%
 47	      7	  0.00%
 48	     13	  0.00%
 49	     31	  0.00%
 50	3362704	100.00%
3362771 reads passed initial QC


criterion=sequence-density
sequence-density=14.12
sequence-density-rank=1
fanout-score=1.52
fanout-score-rank=6
prefix-density=0.06
prefix-fanout=1.5
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGT


criterion=fanout-score
sequence-density=0.82
sequence-density-rank=22
fanout-score=24.24
fanout-score-rank=1
prefix-density=19.65
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 08:27:03
                             Started mapping on |	May 05 08:27:03
                                    Finished on |	May 05 08:27:15
       Mapping speed, Million of reads per hour |	1008.83

                          Number of input reads |	3362771
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2252520
                        Uniquely mapped reads % |	66.98%
                          Average mapped length |	49.61
                       Number of splices: Total |	1243
            Number of splices: Annotated (sjdb) |	598
                       Number of splices: GT/AG |	1123
                       Number of splices: GC/AG |	23
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	97
                      Mismatch rate per base, % |	0.27%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.13
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	367811
             % of reads mapped to multiple loci |	10.94%
        Number of reads mapped to too many loci |	579956
             % of reads mapped to too many loci |	17.25%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.83%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	742440	742440	742440
N_multimapping	367811	367811	367811
N_noFeature	11773	2187157	11805
N_ambiguous	82259	28	17951
UnstrandedReadsAssigned:2158488 PositiveStrandReadsAssigned:65335 NegativeStrandReadsAssigned:2222764
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943854 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943854-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,362,771 reads, 3,028,530 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,116 rounds

 109283 SRR25943854.ke.tsv
  52636 SRR25943854.se.tsv
 161919 total
==> SRR25943854.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943854.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943854 completed mapping pipeline successfully
