Starting /dee2/code/volunteer_pipeline.sh SRR25943844
    current disk space = 1265712568
    free memory = 194810872 
SRR25943844 SRAfilesize
e448004d50525dcdf0a3da63cc4901ce  SRR25943844.sra
SRR25943844.sra file validated
SRR25943844 is single end
SRR25943844 is conventional basespace
SRR25943844 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943844_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.327	32.0	32.0	32.0	32.0	32.0
2	31.489	32.0	32.0	32.0	32.0	32.0
3	31.58425	32.0	32.0	32.0	32.0	32.0
4	31.74125	32.0	32.0	32.0	32.0	32.0
5	31.663	32.0	32.0	32.0	32.0	32.0
6	35.148	36.0	36.0	36.0	36.0	36.0
7	35.12575	36.0	36.0	36.0	36.0	36.0
8	35.08075	36.0	36.0	36.0	36.0	36.0
9	35.048	36.0	36.0	36.0	32.0	36.0
10	35.01925	36.0	36.0	36.0	32.0	36.0
11	35.00175	36.0	36.0	36.0	32.0	36.0
12	35.139	36.0	36.0	36.0	36.0	36.0
13	35.09825	36.0	36.0	36.0	36.0	36.0
14	35.16375	36.0	36.0	36.0	36.0	36.0
15	35.07025	36.0	36.0	36.0	36.0	36.0
16	35.04025	36.0	36.0	36.0	36.0	36.0
17	34.993	36.0	36.0	36.0	36.0	36.0
18	35.15125	36.0	36.0	36.0	36.0	36.0
19	35.107	36.0	36.0	36.0	36.0	36.0
20	35.04325	36.0	36.0	36.0	36.0	36.0
21	35.05425	36.0	36.0	36.0	36.0	36.0
22	35.05325	36.0	36.0	36.0	36.0	36.0
23	35.1455	36.0	36.0	36.0	36.0	36.0
24	35.085	36.0	36.0	36.0	36.0	36.0
25	34.65475	36.0	36.0	36.0	32.0	36.0
26	34.95075	36.0	36.0	36.0	32.0	36.0
27	34.9245	36.0	36.0	36.0	36.0	36.0
28	34.6005	36.0	36.0	36.0	32.0	36.0
29	35.0205	36.0	36.0	36.0	36.0	36.0
30	35.00225	36.0	36.0	36.0	36.0	36.0
31	34.994	36.0	36.0	36.0	36.0	36.0
32	34.9525	36.0	36.0	36.0	32.0	36.0
33	34.85075	36.0	36.0	36.0	32.0	36.0
34	34.91075	36.0	36.0	36.0	32.0	36.0
35	34.97125	36.0	36.0	36.0	36.0	36.0
36	34.97725	36.0	36.0	36.0	36.0	36.0
37	34.89125	36.0	36.0	36.0	32.0	36.0
38	35.02275	36.0	36.0	36.0	32.0	36.0
39	35.002	36.0	36.0	36.0	32.0	36.0
40	34.68	36.0	36.0	36.0	32.0	36.0
41	34.9085	36.0	36.0	36.0	32.0	36.0
42	34.79775	36.0	36.0	36.0	36.0	36.0
43	34.6825	36.0	36.0	36.0	32.0	36.0
44	34.6735	36.0	36.0	36.0	32.0	36.0
45	34.766	36.0	36.0	36.0	32.0	36.0
46	34.668	36.0	36.0	36.0	32.0	36.0
47	34.70875	36.0	36.0	36.0	32.0	36.0
48	34.5645	36.0	36.0	36.0	32.0	36.0
49	34.617	36.0	36.0	36.0	32.0	36.0
50	34.32975	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	2.0
25	5.0
26	11.0
27	14.0
28	46.0
29	62.0
30	72.0
31	136.0
32	188.0
33	277.0
34	592.0
35	2594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.95	14.025000000000002	25.775	26.25
2	26.275	22.2	25.4	26.125
3	38.85	19.950000000000003	18.175	23.025000000000002
4	28.050000000000004	20.175	22.875	28.9
5	16.175	22.85	34.075	26.900000000000002
6	28.182045511377847	27.93198299574894	25.656414103525883	18.229557389347338
7	33.324999999999996	15.975	31.6	19.1
8	23.849999999999998	18.45	20.025000000000002	37.675
9	21.099999999999998	21.349999999999998	28.249999999999996	29.299999999999997
10	25.2	14.774999999999999	34.625	25.4
11	21.175	41.425	14.224999999999998	23.175
12	37.974999999999994	19.7	21.6	20.724999999999998
13	36.725	29.4	16.525000000000002	17.349999999999998
14	17.575	20.075000000000003	31.35	31.0
15	15.8	39.625	18.099999999999998	26.474999999999998
16	13.350000000000001	35.075	26.775	24.8
17	18.7	25.6	23.974999999999998	31.724999999999998
18	20.9	18.825	37.2	23.075000000000003
19	22.900000000000002	26.35	30.5	20.25
20	33.725	17.849999999999998	23.0	25.424999999999997
21	15.0	16.625	41.0	27.375
22	23.1	17.724999999999998	33.25	25.924999999999997
23	34.599999999999994	13.425	24.325	27.650000000000002
24	25.15	21.75	25.575	27.525
25	1.25	4.8	83.15	10.8
26	13.725000000000001	30.3	23.825	32.15
27	28.9	28.525	17.4	25.174999999999997
28	14.299999999999999	23.925	37.4	24.375
29	27.224999999999998	24.625	14.075	34.075
30	20.75	18.25	35.9	25.1
31	36.25	21.95	16.150000000000002	25.650000000000002
32	31.35	19.475	20.3	28.875
33	28.9	24.474999999999998	19.35	27.275
34	20.775	25.2	30.95	23.075000000000003
35	22.45	17.45	34.5	25.6
36	26.950000000000003	11.95	37.925	23.175
37	25.474999999999998	31.474999999999998	24.925	18.125
38	33.0	11.0	20.9	35.099999999999994
39	31.25	26.325	17.95	24.474999999999998
40	20.225	29.625	19.625	30.525000000000002
41	19.2	26.325	25.624999999999996	28.849999999999998
42	27.3	17.7	30.925000000000004	24.075
43	26.575	20.05	22.125	31.25
44	23.925	15.65	30.425	30.0
45	13.55	25.75	21.55	39.15
46	20.200000000000003	29.525000000000002	29.5	20.775
47	31.7	13.425	16.575	38.3
48	16.775000000000002	22.075	33.175	27.975
49	28.65	26.875	24.325	20.150000000000002
50	14.325	25.650000000000002	19.475	40.550000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	15.0
30	30.0
31	16.5
32	3.0
33	2.5
34	2.0
35	4.5
36	7.0
37	19.0
38	31.0
39	74.5
40	118.0
41	216.5
42	315.0
43	356.0
44	397.0
45	348.0
46	299.0
47	230.0
48	161.0
49	437.0
50	713.0
51	623.0
52	533.0
53	363.5
54	194.0
55	298.5
56	403.0
57	351.5
58	300.0
59	302.5
60	305.0
61	202.5
62	100.0
63	75.5
64	51.0
65	33.0
66	15.0
67	11.5
68	8.0
69	9.0
70	10.0
71	7.0
72	4.0
73	2.0
74	0.0
75	0.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17398508699254	21.175
2	13.173156586578294	7.95
3	4.722452361226181	4.275
4	2.071251035625518	2.5
5	2.071251035625518	3.125
6	1.4084507042253522	2.55
7	0.579950289975145	1.225
8	0.4142502071251036	1.0
9	0.579950289975145	1.575
>10	3.8939519469759736	21.8
>50	0.579950289975145	12.525
>100	0.33140016570008285	20.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	272	6.800000000000001	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	250	6.25	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	188	4.7	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	102	2.55	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	86	2.15	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	82	2.0500000000000003	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	81	2.025	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	76	1.9	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	62	1.55	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	59	1.4749999999999999	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	55	1.375	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	47	1.175	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	44	1.0999999999999999	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	32	0.8	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	31	0.775	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	31	0.775	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	29	0.7250000000000001	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	29	0.7250000000000001	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	29	0.7250000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	29	0.7250000000000001	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	25	0.625	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	24	0.6	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	24	0.6	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	23	0.575	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	22	0.5499999999999999	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	21	0.525	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	20	0.5	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	20	0.5	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	19	0.475	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	19	0.475	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	17	0.42500000000000004	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	17	0.42500000000000004	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	16	0.4	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	16	0.4	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	16	0.4	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	16	0.4	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	16	0.4	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	14	0.35000000000000003	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	14	0.35000000000000003	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	13	0.325	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	13	0.325	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	13	0.325	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	13	0.325	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	13	0.325	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	11	0.27499999999999997	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	11	0.27499999999999997	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	11	0.27499999999999997	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	11	0.27499999999999997	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	11	0.27499999999999997	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	11	0.27499999999999997	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	11	0.27499999999999997	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	10	0.25	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	10	0.25	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	10	0.25	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	10	0.25	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	10	0.25	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	10	0.25	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	10	0.25	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	9	0.22499999999999998	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	9	0.22499999999999998	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	9	0.22499999999999998	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	9	0.22499999999999998	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	9	0.22499999999999998	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	9	0.22499999999999998	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	9	0.22499999999999998	No Hit
TGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTC	8	0.2	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	8	0.2	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	8	0.2	No Hit
CAGGTACAAGGCAAGAGGATTTGCCCCAGCAAGCCAACCATTCTCTGAAC	8	0.2	No Hit
GCCATGAGGTCCACCACCCTGTTGCTGTAGCCGTATTCATTGTCATACCA	8	0.2	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	7	0.17500000000000002	No Hit
AGCACTTTACAATGAGGGCTACAATGAATGTCACAAAAGCAAGCAGGAAT	7	0.17500000000000002	No Hit
AGCTCGTCCTCTGTGGTCTTCTGGTAGACTGGGTTGTCAAAGTTTATGCT	7	0.17500000000000002	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	7	0.17500000000000002	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	7	0.17500000000000002	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	7	0.17500000000000002	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	6	0.15	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	6	0.15	No Hit
TTTCTTGATCACGGTCTTGTATGCTTTCTCGAAGTCCTTGGCCAGGACAA	6	0.15	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	6	0.15	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	6	0.15	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	6	0.15	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	6	0.15	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	6	0.15	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	6	0.15	No Hit
GTGGCTCATTCCAGAAGCTGAACTCCAGAAGCAACTGGTTTCTCCTGGAC	6	0.15	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	6	0.15	No Hit
GAGACAGGATGCTTTCCTAAGGTTTTACAAAAGTCCACCAGAGATTCAAA	6	0.15	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	6	0.15	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	6	0.15	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	6	0.15	No Hit
GCTCTTACATCACTGCTCTGTGTCTTCTCCAATATCCAGTCCATGTACTC	6	0.15	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	6	0.15	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	5	0.125	No Hit
GCGCAGGCGGGACACATCTTTACACTGCTTGCTGCTCTTGGGATTGAAGT	5	0.125	No Hit
TGGTCAGCCTCCTGGATGGTCCTGTCCGCAATACTGCCCAGCTGCTCATC	5	0.125	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	5	0.125	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	5	0.125	No Hit
CCTTAAAGGTGTTCAGTTTGTGGTTAGCAATGCGCCCAGCCACCACAATC	5	0.125	No Hit
GGAACAGCGTCATTAGCACCTTGCCTACAGCCACCTCCAAGGCCACCTGC	5	0.125	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	5	0.125	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATGGACTTCTCAGTGAC	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCAT	5	0.125	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	5	0.125	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	5	0.125	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	5	0.125	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	5	0.125	No Hit
CACCCAGCCCGTTACTTCATGCTTTTCTGGACCACCCCGGGTCTGAATGG	5	0.125	No Hit
GTGTGATGCCTGCAATTTGATGGGTAGAGCAGCCAATGAAGAAGAGCGGC	5	0.125	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	5	0.125	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	5	0.125	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	5	0.125	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	5	0.125	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	5	0.125	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	5	0.125	No Hit
CTTGGAGAGATTGAAATAGGTCTCTTTGATATCAAAGTCCTTGTGGATGA	5	0.125	No Hit
CCCAAGTAGGTGATGCCAGAATCCTCGTAGAAGCTAGCACTGGTGTTGAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	45	2.728484E-10	44.000004	6
TGTTGGC	20	6.952051E-4	44.000004	14
GAGGTAT	45	2.728484E-10	44.000004	1
GTGTCCT	20	6.952051E-4	44.000004	37
ACATGTG	45	2.728484E-10	44.000004	16
TCCTAGG	45	2.728484E-10	44.000004	7
GGACTTC	40	5.315087E-9	44.000004	37
TGTCTGT	20	6.952051E-4	44.000004	10
GCGGCCA	20	6.952051E-4	44.000004	3
CCATGTC	20	6.952051E-4	44.000004	7
ATTGGAC	45	2.728484E-10	44.000004	34
GTCTGTT	20	6.952051E-4	44.000004	11
GTATCCT	45	2.728484E-10	44.000004	4
CTAGGTA	45	2.728484E-10	44.000004	9
ATGTGGA	45	2.728484E-10	44.000004	18
GGATAGA	40	5.315087E-9	44.000004	22
TCTGTTG	20	6.952051E-4	44.000004	12
TTCCTGG	20	6.952051E-4	44.000004	21
TCAGTGA	45	2.728484E-10	44.000004	44
CTGGTCA	20	6.952051E-4	44.000004	24
>>END_MODULE
Read 454281 spots for SRR25943844.sra
Written 454281 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
Read 454275 spots for SRR25943844.sra
Written 454275 spots for SRR25943844.sra
SRR ids: ['SRR25943844.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ahre0okc
SRR25943844.sra spots: 3634206
blocks: [[1, 454275], [454276, 908550], [908551, 1362825], [1362826, 1817100], [1817101, 2271375], [2271376, 2725650], [2725651, 3179925], [3179926, 3634206]]
SRR25943844 file size 627788
SRR25943844 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943844 SRR25943844_1.fastq
Input file:	SRR25943844_1.fastq
trimmed:	SRR25943844-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:37:12 2026 >> started

Tue May  5 05:37:13 2026 >> done (1.102s)
3634206 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3634206 (100.00%) reads available; of these:
     87 ( 0.00%) trimmed reads available after processing
3634119 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      2	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      4	  0.00%
 44	      1	  0.00%
 45	      1	  0.00%
 46	     17	  0.00%
 47	     11	  0.00%
 48	     20	  0.00%
 49	     31	  0.00%
 50	3634119	100.00%
3634206 reads passed initial QC


criterion=sequence-density
sequence-density=8.63
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=22
prefix-density=0.00
prefix-fanout=1.0
sequence=GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGACCT


criterion=fanout-score
sequence-density=0.37
sequence-density-rank=43
fanout-score=20.42
fanout-score-rank=1
prefix-density=7.61
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:37:19
                             Started mapping on |	May 05 05:37:20
                                    Finished on |	May 05 05:37:25
       Mapping speed, Million of reads per hour |	2616.63

                          Number of input reads |	3634206
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2572892
                        Uniquely mapped reads % |	70.80%
                          Average mapped length |	49.56
                       Number of splices: Total |	1445
            Number of splices: Annotated (sjdb) |	694
                       Number of splices: GT/AG |	1269
                       Number of splices: GC/AG |	28
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	147
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.07
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	388741
             % of reads mapped to multiple loci |	10.70%
        Number of reads mapped to too many loci |	606679
             % of reads mapped to too many loci |	16.69%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.81%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	672573	672573	672573
N_multimapping	388741	388741	388741
N_noFeature	10872	2523812	10877
N_ambiguous	71929	42	23418
UnstrandedReadsAssigned:2490091 PositiveStrandReadsAssigned:49038 NegativeStrandReadsAssigned:2538597
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943844 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943844-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,634,206 reads, 3,426,383 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,136 rounds

 109283 SRR25943844.ke.tsv
  52636 SRR25943844.se.tsv
 161919 total
==> SRR25943844.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943844.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943844 completed mapping pipeline successfully
