Starting /dee2/code/volunteer_pipeline.sh SRR25943821
    current disk space = 1264085104
    free memory = 195155752 
SRR25943821 SRAfilesize
133386518e7b2df3a95f65a2f059e064  SRR25943821.sra
SRR25943821.sra file validated
SRR25943821 is single end
SRR25943821 is conventional basespace
SRR25943821 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.379	32.0	32.0	32.0	32.0	32.0
2	31.553	32.0	32.0	32.0	32.0	32.0
3	31.58375	32.0	32.0	32.0	32.0	32.0
4	31.65275	32.0	32.0	32.0	32.0	32.0
5	31.61975	32.0	32.0	32.0	32.0	32.0
6	35.233	36.0	36.0	36.0	36.0	36.0
7	35.2105	36.0	36.0	36.0	36.0	36.0
8	35.04175	36.0	36.0	36.0	36.0	36.0
9	35.1775	36.0	36.0	36.0	36.0	36.0
10	35.092	36.0	36.0	36.0	36.0	36.0
11	35.272	36.0	36.0	36.0	36.0	36.0
12	35.2025	36.0	36.0	36.0	36.0	36.0
13	35.24275	36.0	36.0	36.0	36.0	36.0
14	35.17575	36.0	36.0	36.0	36.0	36.0
15	35.132	36.0	36.0	36.0	36.0	36.0
16	35.104	36.0	36.0	36.0	36.0	36.0
17	35.1015	36.0	36.0	36.0	36.0	36.0
18	35.11675	36.0	36.0	36.0	36.0	36.0
19	35.18325	36.0	36.0	36.0	36.0	36.0
20	35.11125	36.0	36.0	36.0	36.0	36.0
21	35.10525	36.0	36.0	36.0	36.0	36.0
22	35.29025	36.0	36.0	36.0	36.0	36.0
23	35.274	36.0	36.0	36.0	36.0	36.0
24	35.146	36.0	36.0	36.0	36.0	36.0
25	35.28175	36.0	36.0	36.0	36.0	36.0
26	34.93925	36.0	36.0	36.0	36.0	36.0
27	35.22725	36.0	36.0	36.0	36.0	36.0
28	34.90025	36.0	36.0	36.0	32.0	36.0
29	35.18725	36.0	36.0	36.0	36.0	36.0
30	34.98625	36.0	36.0	36.0	36.0	36.0
31	35.18875	36.0	36.0	36.0	36.0	36.0
32	34.961	36.0	36.0	36.0	36.0	36.0
33	34.952	36.0	36.0	36.0	32.0	36.0
34	35.06275	36.0	36.0	36.0	36.0	36.0
35	35.18875	36.0	36.0	36.0	36.0	36.0
36	35.0535	36.0	36.0	36.0	36.0	36.0
37	34.98725	36.0	36.0	36.0	36.0	36.0
38	34.9945	36.0	36.0	36.0	36.0	36.0
39	35.086	36.0	36.0	36.0	36.0	36.0
40	34.759	36.0	36.0	36.0	32.0	36.0
41	34.925	36.0	36.0	36.0	36.0	36.0
42	34.86575	36.0	36.0	36.0	32.0	36.0
43	34.7975	36.0	36.0	36.0	32.0	36.0
44	34.83075	36.0	36.0	36.0	36.0	36.0
45	34.763	36.0	36.0	36.0	32.0	36.0
46	34.67775	36.0	36.0	36.0	32.0	36.0
47	34.78925	36.0	36.0	36.0	36.0	36.0
48	34.417	36.0	36.0	36.0	32.0	36.0
49	34.803	36.0	36.0	36.0	32.0	36.0
50	34.31475	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	3.0
25	5.0
26	10.0
27	23.0
28	26.0
29	47.0
30	81.0
31	102.0
32	146.0
33	285.0
34	640.0
35	2631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.75	18.3	31.924999999999997	23.025000000000002
2	27.375	20.8	24.8	27.025
3	33.324999999999996	22.625	24.25	19.8
4	24.224999999999998	20.5	24.349999999999998	30.925000000000004
5	12.25	23.925	30.625000000000004	33.2
6	28.75718929732433	22.405601400350086	29.457364341085274	19.379844961240313
7	33.050000000000004	17.599999999999998	29.125	20.225
8	20.599999999999998	21.075	27.025	31.3
9	23.150000000000002	17.05	30.25	29.549999999999997
10	28.225	17.299999999999997	27.250000000000004	27.224999999999998
11	25.2	31.8	16.05	26.950000000000003
12	32.1	24.525	20.25	23.125
13	29.257314328582147	33.9584896224056	18.904726181545385	17.879469867466867
14	20.875	21.05	23.625	34.449999999999996
15	15.65	34.825	22.2	27.325
16	16.375	26.150000000000002	25.174999999999997	32.300000000000004
17	21.8	28.599999999999998	19.85	29.75
18	21.025	16.025	35.125	27.825
19	22.325	30.425	26.85	20.4
20	34.425	14.774999999999999	19.225	31.574999999999996
21	16.8	18.625	28.625	35.949999999999996
22	18.925	19.375	35.675000000000004	26.025
23	33.900000000000006	14.399999999999999	21.525	30.175
24	31.025000000000002	15.5	20.849999999999998	32.625
25	1.275	6.05	80.9	11.774999999999999
26	17.125	28.425	18.325	36.125
27	26.424999999999997	29.849999999999998	18.625	25.1
28	14.549999999999999	23.275000000000002	34.025	28.15
29	28.075	29.849999999999998	14.149999999999999	27.925
30	22.15	21.375	25.25	31.225
31	31.624999999999996	23.625	16.125	28.625
32	29.575000000000003	20.325	19.525000000000002	30.575000000000003
33	28.875	20.849999999999998	19.625	30.65
34	20.275000000000002	19.125	32.65	27.950000000000003
35	23.9	18.625	31.825	25.650000000000002
36	27.500000000000004	11.825	35.475	25.2
37	20.200000000000003	35.675000000000004	23.825	20.3
38	29.675	12.375	21.65	36.3
39	33.275	23.3	20.9	22.525000000000002
40	19.1	33.074999999999996	22.55	25.275
41	19.8	31.374999999999996	19.400000000000002	29.425
42	24.33108277069267	19.079769942485623	30.807701925481368	25.78144536134033
43	31.974999999999998	23.05	23.425	21.55
44	25.074999999999996	17.175	22.475	35.275
45	14.053513378344586	24.781195298824706	19.929982495623904	41.2353088272068
46	18.625	32.025	29.875	19.475
47	25.224999999999998	16.150000000000002	16.975	41.65
48	22.05551387846962	28.307076769192296	20.78019504876219	28.857214303575894
49	25.256314078519633	25.98149537384346	28.032008002000502	20.730182545636406
50	16.5	24.9	19.275000000000002	39.324999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	4.0
30	8.0
31	4.5
32	1.0
33	0.5
34	0.0
35	6.0
36	12.0
37	22.0
38	32.0
39	48.5
40	65.0
41	165.5
42	266.0
43	238.0
44	210.0
45	236.0
46	262.0
47	219.5
48	177.0
49	524.0
50	871.0
51	720.5
52	570.0
53	425.0
54	280.0
55	387.0
56	494.0
57	379.5
58	265.0
59	258.0
60	251.0
61	174.5
62	98.0
63	86.0
64	74.0
65	49.0
66	24.0
67	18.5
68	13.0
69	14.0
70	15.0
71	13.5
72	12.0
73	6.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.025
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.025
43	0.0
44	0.0
45	0.025
46	0.0
47	0.0
48	0.025
49	0.025
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06870799103808	24.125
2	11.053024645257654	7.3999999999999995
3	5.37714712471994	5.4
4	2.7632561613144135	3.6999999999999997
5	1.8670649738610903	3.125
6	1.1202389843166543	2.25
7	0.597460791635549	1.4000000000000001
8	0.37341299477221807	1.0
9	0.4480955937266617	1.35
>10	3.51008215085885	21.925
>50	0.5227781926811054	11.1
>100	0.2987303958177745	17.224999999999998
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	333	8.325000000000001	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	126	3.15	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	123	3.075	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	107	2.675	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	75	1.875	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	73	1.825	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	69	1.725	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	63	1.575	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	60	1.5	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	52	1.3	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	52	1.3	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	48	1.2	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	45	1.125	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	44	1.0999999999999999	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	43	1.075	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	37	0.9249999999999999	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	34	0.8500000000000001	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	33	0.8250000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	32	0.8	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	31	0.775	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	27	0.675	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	26	0.65	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	21	0.525	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	21	0.525	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	19	0.475	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	19	0.475	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	18	0.44999999999999996	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	17	0.42500000000000004	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	15	0.375	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	15	0.375	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	15	0.375	No Hit
GATATTTGTCCTCATGGAAGGACGTAGCCTATAAATGCTGTGGCTATGAC	14	0.35000000000000003	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	14	0.35000000000000003	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	14	0.35000000000000003	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	14	0.35000000000000003	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	13	0.325	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	13	0.325	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	13	0.325	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	13	0.325	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	12	0.3	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	12	0.3	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	12	0.3	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	12	0.3	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	12	0.3	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	12	0.3	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	12	0.3	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	11	0.27499999999999997	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	11	0.27499999999999997	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	11	0.27499999999999997	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	11	0.27499999999999997	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	11	0.27499999999999997	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	10	0.25	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	10	0.25	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	10	0.25	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	10	0.25	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	10	0.25	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	10	0.25	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	10	0.25	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	9	0.22499999999999998	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	9	0.22499999999999998	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	9	0.22499999999999998	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	9	0.22499999999999998	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	9	0.22499999999999998	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	9	0.22499999999999998	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	8	0.2	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	8	0.2	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	8	0.2	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	8	0.2	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	8	0.2	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	7	0.17500000000000002	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	7	0.17500000000000002	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	7	0.17500000000000002	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	7	0.17500000000000002	No Hit
CACCCATGACCATGAGAAATCTGCTCAGCGAGGAGTAGAAGTAAAGGATG	7	0.17500000000000002	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	7	0.17500000000000002	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	7	0.17500000000000002	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	7	0.17500000000000002	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	6	0.15	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	6	0.15	No Hit
TGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAG	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	6	0.15	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	6	0.15	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	6	0.15	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	6	0.15	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	6	0.15	No Hit
CCAGCTGCCCTTGAGTGATGTACTTTTTCCACCAGAGGTAGGGACGCATG	6	0.15	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	6	0.15	No Hit
CTCCTTCTTCGAGACTTTCACAATTTTTATGCTGCTGCCCGAAGTCTTCT	6	0.15	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	6	0.15	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	6	0.15	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	6	0.15	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	5	0.125	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	5	0.125	No Hit
TAAGTACGGTCTTCATAGTGAACCTCCTTGAACAGAAGTTGAAGAGGTCT	5	0.125	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	5	0.125	No Hit
TTCTGCCATCAGCTGTCGTTTCATCAAACTTCTCTCCCAGGTTACAAGAG	5	0.125	No Hit
TAAGATCTCCGTCGTAGATTCCTTTGCTGTAGATGTATAAGAGGAGAGGC	5	0.125	No Hit
TCTTCCACACTGATCACAGAGACCTTGGAGGTCAGGTCCACGCCCAGGTC	5	0.125	No Hit
AAACGCCCACTTTGACATAGACTTTATAGACCATACCACCAATACCGAAA	5	0.125	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	5	0.125	No Hit
GACAGATCCGAAGCACCTCTTCTTTTGTAGAAGTTGATAGATGGTTCATT	5	0.125	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	5	0.125	No Hit
TCACTTCGCACTTCTCAAACAATGTAAAATTGGGCTCGAACCCGCCACCA	5	0.125	No Hit
AGAGCTTTGTGATTCCCATGGACTTAAGGACCTCCACCAGGTTGTAATTC	5	0.125	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	5	0.125	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	5	0.125	No Hit
GTACTTTGACAGCTTCCACCTCATTGGCTGTTGAGTCCATCTTAGCAATG	5	0.125	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	5	0.125	No Hit
ATTAACAAGCAATCAACCCTCAGCTCAAGATGATAACAATCAAGGTCACA	5	0.125	No Hit
CCTGGCTCAGTGCAGTTCCGCAAATGTAAGTCCTCCTTGGTGAAGCACAG	5	0.125	No Hit
ACTGGTGAACTCGTAGGCAGGCACTTCCAGGTTGAGAGGTGCAGGGCCCT	5	0.125	No Hit
GGACAATCCTCCTCAGCAGCCTGCTGCTTACAGCAGATGGCGTCAACACA	5	0.125	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	5	0.125	No Hit
CAGGTACAAGGCAAGAGGATTTGCCCCAGCAAGCCAACCATTCTCTGAAC	5	0.125	No Hit
TCTCCATCGCCGTCTTTGTCAAACTCCTCTATCATGGCCCGGAGCTCCTC	5	0.125	No Hit
GTCAACCTCATGTGGCAGGTTCCCAATGAAGAGCTGGTGACTGTCAGGGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGAA	65	0.0	44.000004	6
GTTGAAA	65	0.0	44.000004	7
GTTTGCC	20	6.952051E-4	44.000004	29
GCCTTAC	65	0.0	44.000004	32
ATCTTCT	20	6.952051E-4	44.000004	15
CACACAG	65	0.0	44.000004	14
GAAACAC	65	0.0	44.000004	10
CCTGTTG	65	0.0	44.000004	4
GCGATGC	20	6.952051E-4	44.000004	41
TGGTAAT	65	0.0	44.000004	22
AAACACA	65	0.0	44.000004	11
CCTGTAT	20	6.952051E-4	44.000004	10
GCTGGTA	65	0.0	44.000004	20
TACGGCC	65	0.0	44.000004	28
GTATCTT	20	6.952051E-4	44.000004	13
GATGCAT	20	6.952051E-4	44.000004	43
GGCGATG	20	6.952051E-4	44.000004	40
GGTTTGC	20	6.952051E-4	44.000004	28
GAATGCC	65	0.0	44.000004	39
ACACAGC	65	0.0	44.000004	15
>>END_MODULE
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410912 spots for SRR25943821.sra
Written 410912 spots for SRR25943821.sra
Read 410919 spots for SRR25943821.sra
Written 410919 spots for SRR25943821.sra
SRR ids: ['SRR25943821.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_60i7r62s
SRR25943821.sra spots: 3287303
blocks: [[1, 410912], [410913, 821824], [821825, 1232736], [1232737, 1643648], [1643649, 2054560], [2054561, 2465472], [2465473, 2876384], [2876385, 3287303]]
SRR25943821 file size 567760
SRR25943821 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943821 SRR25943821_1.fastq
Input file:	SRR25943821_1.fastq
trimmed:	SRR25943821-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:44:41 2026 >> started

Tue May  5 05:44:42 2026 >> done (0.865s)
3287303 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3287303 (100.00%) reads available; of these:
     66 ( 0.00%) trimmed reads available after processing
3287237 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      2	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      2	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	      9	  0.00%
 47	     10	  0.00%
 48	     11	  0.00%
 49	     31	  0.00%
 50	3287237	100.00%
3287303 reads passed initial QC


criterion=sequence-density
sequence-density=8.48
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAACCT


criterion=fanout-score
sequence-density=1.93
sequence-density-rank=9
fanout-score=13.55
fanout-score-rank=1
prefix-density=2.02
prefix-fanout=13.0
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTCGTAT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:44:48
                             Started mapping on |	May 05 05:44:48
                                    Finished on |	May 05 05:44:53
       Mapping speed, Million of reads per hour |	2366.86

                          Number of input reads |	3287303
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2598031
                        Uniquely mapped reads % |	79.03%
                          Average mapped length |	49.54
                       Number of splices: Total |	1850
            Number of splices: Annotated (sjdb) |	695
                       Number of splices: GT/AG |	1713
                       Number of splices: GC/AG |	25
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	112
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.11
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	337446
             % of reads mapped to multiple loci |	10.27%
        Number of reads mapped to too many loci |	243325
             % of reads mapped to too many loci |	7.40%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.30%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	351826	351826	351826
N_multimapping	337446	337446	337446
N_noFeature	21897	2549944	21919
N_ambiguous	70255	34	23025
UnstrandedReadsAssigned:2505879 PositiveStrandReadsAssigned:48053 NegativeStrandReadsAssigned:2553087
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943821 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943821-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,287,303 reads, 3,018,710 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,168 rounds

 109283 SRR25943821.ke.tsv
  52636 SRR25943821.se.tsv
 161919 total
==> SRR25943821.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943821.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943821 completed mapping pipeline successfully
