Starting /dee2/code/volunteer_pipeline.sh SRR25943839
    current disk space = 1263869836
    free memory = 194565008 
SRR25943839 SRAfilesize
377aaad84c595523d5f28c9548c4ac8d  SRR25943839.sra
SRR25943839.sra file validated
SRR25943839 is single end
SRR25943839 is conventional basespace
SRR25943839 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943839_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4595	32.0	32.0	32.0	32.0	32.0
2	31.54375	32.0	32.0	32.0	32.0	32.0
3	31.627	32.0	32.0	32.0	32.0	32.0
4	31.5955	32.0	32.0	32.0	32.0	32.0
5	31.58425	32.0	32.0	32.0	32.0	32.0
6	35.04375	36.0	36.0	36.0	36.0	36.0
7	35.16825	36.0	36.0	36.0	36.0	36.0
8	34.9255	36.0	36.0	36.0	32.0	36.0
9	34.893	36.0	36.0	36.0	32.0	36.0
10	34.96075	36.0	36.0	36.0	32.0	36.0
11	34.9185	36.0	36.0	36.0	32.0	36.0
12	34.951	36.0	36.0	36.0	36.0	36.0
13	35.1445	36.0	36.0	36.0	36.0	36.0
14	35.11225	36.0	36.0	36.0	36.0	36.0
15	35.086	36.0	36.0	36.0	36.0	36.0
16	34.93175	36.0	36.0	36.0	36.0	36.0
17	34.9925	36.0	36.0	36.0	36.0	36.0
18	34.868	36.0	36.0	36.0	36.0	36.0
19	35.0505	36.0	36.0	36.0	36.0	36.0
20	35.112	36.0	36.0	36.0	36.0	36.0
21	34.98425	36.0	36.0	36.0	36.0	36.0
22	34.86775	36.0	36.0	36.0	36.0	36.0
23	35.07525	36.0	36.0	36.0	36.0	36.0
24	34.9615	36.0	36.0	36.0	36.0	36.0
25	35.078	36.0	36.0	36.0	36.0	36.0
26	34.834	36.0	36.0	36.0	32.0	36.0
27	34.83425	36.0	36.0	36.0	32.0	36.0
28	34.69525	36.0	36.0	36.0	32.0	36.0
29	34.93475	36.0	36.0	36.0	36.0	36.0
30	34.79375	36.0	36.0	36.0	32.0	36.0
31	34.9195	36.0	36.0	36.0	36.0	36.0
32	34.8525	36.0	36.0	36.0	32.0	36.0
33	34.8425	36.0	36.0	36.0	32.0	36.0
34	34.77275	36.0	36.0	36.0	32.0	36.0
35	34.8235	36.0	36.0	36.0	32.0	36.0
36	34.89725	36.0	36.0	36.0	32.0	36.0
37	34.764	36.0	36.0	36.0	32.0	36.0
38	34.734	36.0	36.0	36.0	32.0	36.0
39	34.61175	36.0	36.0	36.0	32.0	36.0
40	34.27675	36.0	36.0	36.0	32.0	36.0
41	34.52125	36.0	36.0	36.0	32.0	36.0
42	34.7265	36.0	36.0	36.0	32.0	36.0
43	34.36625	36.0	36.0	36.0	32.0	36.0
44	34.556	36.0	36.0	36.0	32.0	36.0
45	34.231	36.0	36.0	36.0	32.0	36.0
46	34.27975	36.0	36.0	36.0	32.0	36.0
47	34.29675	36.0	36.0	36.0	32.0	36.0
48	34.17775	36.0	36.0	36.0	32.0	36.0
49	34.3885	36.0	36.0	36.0	32.0	36.0
50	33.9625	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.5014553729723978
11101	2	-0.16814850096584166
11101	3	-0.03668862956788743
11101	4	-0.17660289486914849
11101	5	0.154322457728032
11101	6	-0.002024291497974673
11101	7	0.07828583525177635
11101	8	0.047498081553811744
11101	9	0.027691778465772643
11101	10	-0.33019766611098333
11101	11	-0.035802175121062874
11101	12	-0.010028842846182329
11101	13	0.17447275806409124
11101	14	0.10020904448148826
11101	15	-0.27079198751025046
11101	16	-0.18155116297531038
11101	17	-0.10280225450503622
11101	18	-0.45668280807599615
11101	19	0.01717340107433074
11101	20	-0.1864729697547034
11101	21	0.2363393400545135
11101	22	0.009089465745809377
11101	23	-0.09643830541663334
11101	24	-0.16630943875525617
11101	25	0.026196713503210844
11101	26	0.04204704823899874
11101	27	0.015003572279113087
11101	28	0.22764679421026557
11101	29	-0.053266650789865366
11101	30	-0.2999391389484245
11101	31	-0.13504538117540932
11101	32	-0.11824243867587825
11101	33	-0.05363710936465793
11101	34	0.04711439231562764
11101	35	0.24886877828053855
11101	36	0.4602683178534548
11101	37	0.3188192955994822
11101	38	-0.05417956656346945
11101	39	-0.5742107909290581
11101	40	-0.008560239210396503
11101	41	-0.18619512582360898
11101	42	0.09061681352702777
11101	43	-0.20492974517742368
11101	44	-0.017662935619590314
11101	45	-0.10616284300494527
11101	46	-0.11562276732555432
11101	47	0.5824402635548083
11101	48	-0.25716440422322506
11101	49	0.3699955015744436
11101	50	0.16495991108994446
11102	1	-0.5014553729724014
11102	2	0.1681485009658381
11102	3	0.03668862956788743
11102	4	0.17660289486914849
11102	5	-0.15432245772802844
11102	6	0.002024291497974673
11102	7	-0.07828583525178345
11102	8	-0.04749808155380464
11102	9	-0.027691778465772643
11102	10	0.33019766611097623
11102	11	0.03580217512105577
11102	12	0.010028842846175223
11102	13	-0.17447275806409124
11102	14	-0.10020904448149537
11102	15	0.27079198751025757
11102	16	0.18155116297531038
11102	17	0.10280225450504332
11102	18	0.45668280807599615
11102	19	-0.01717340107433074
11102	20	0.1864729697547034
11102	21	-0.2363393400545064
11102	22	-0.009089465745816483
11102	23	0.09643830541663334
11102	24	0.16630943875525617
11102	25	-0.02619671350321795
11102	26	-0.04204704823899874
11102	27	-0.015003572279113087
11102	28	-0.22764679421025846
11102	29	0.05326665078987247
11102	30	0.2999391389484316
11102	31	0.13504538117541642
11102	32	0.11824243867587114
11102	33	0.05363710936466504
11102	34	-0.047114392315634746
11102	35	-0.24886877828054565
11102	36	-0.4602683178534619
11102	37	-0.3188192955994751
11102	38	0.05417956656346945
11102	39	0.5742107909290581
11102	40	0.008560239210389398
11102	41	0.18619512582360898
11102	42	-0.09061681352703488
11102	43	0.20492974517742368
11102	44	0.017662935619590314
11102	45	0.10616284300495238
11102	46	0.11562276732555432
11102	47	-0.5824402635548154
11102	48	0.25716440422322506
11102	49	-0.3699955015744507
11102	50	-0.16495991108993735
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	5.0
24	5.0
25	11.0
26	25.0
27	26.0
28	49.0
29	60.0
30	95.0
31	138.0
32	172.0
33	311.0
34	575.0
35	2528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.3	22.25	26.25	27.200000000000003
2	21.275	17.625	24.175	36.925000000000004
3	20.175	22.575	23.775	33.475
4	18.85	24.0	27.375	29.775000000000002
5	15.5	26.200000000000003	24.65	33.650000000000006
6	28.65	31.95	20.125	19.275000000000002
7	21.925	25.775	31.55	20.75
8	19.75	21.0	21.25	38.0
9	24.2	18.2	24.775	32.824999999999996
10	22.3	22.6	28.249999999999996	26.85
11	23.200000000000003	18.775	22.75	35.275
12	23.3	18.0	26.35	32.35
13	21.65	23.225	34.5	20.625
14	22.3	17.5	26.6	33.6
15	24.825	22.825	21.475	30.875000000000004
16	18.625	21.275	26.825	33.275
17	17.375	21.425	27.1	34.1
18	22.175	15.2	40.2	22.425
19	25.85	22.5	30.325000000000003	21.325
20	27.950000000000003	16.425	29.45	26.174999999999997
21	20.7	15.925	23.724999999999998	39.65
22	15.1	24.349999999999998	38.15	22.400000000000002
23	28.449999999999996	12.85	24.625	34.075
24	27.725	12.65	21.2	38.425
25	1.525	4.6	82.25	11.625
26	20.175	18.675	28.1	33.050000000000004
27	28.325	17.925	26.400000000000002	27.35
28	12.55	23.925	32.225	31.3
29	24.325	25.474999999999998	23.175	27.025
30	24.025	18.4	26.200000000000003	31.374999999999996
31	18.95	29.65	29.325000000000003	22.075
32	22.725	20.549999999999997	21.55	35.175
33	33.75	15.6	25.124999999999996	25.525
34	27.05	17.125	31.55	24.275
35	25.25	19.825	22.25	32.675
36	21.875	17.4	20.8	39.925
37	18.15	25.775	27.425	28.65
38	28.9	13.65	22.475	34.975
39	29.525000000000002	17.925	26.075	26.474999999999998
40	20.825	24.2	29.549999999999997	25.424999999999997
41	20.95	22.15	21.075	35.825
42	26.0	22.35	19.525000000000002	32.125
43	25.174999999999997	22.15	28.825	23.849999999999998
44	24.775	21.525	19.6	34.1
45	18.575	21.45	24.325	35.65
46	21.025	22.75	29.599999999999998	26.625
47	28.050000000000004	18.25	18.075	35.625
48	22.0	26.950000000000003	16.525000000000002	34.525
49	25.95	22.1	29.95	22.0
50	24.075	13.8	18.3	43.824999999999996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	1.5
32	3.0
33	3.0
34	3.0
35	5.5
36	8.0
37	22.0
38	36.0
39	38.5
40	41.0
41	104.0
42	167.0
43	136.0
44	105.0
45	166.5
46	228.0
47	247.0
48	266.0
49	437.5
50	609.0
51	736.5
52	864.0
53	619.5
54	375.0
55	412.5
56	450.0
57	409.5
58	369.0
59	276.0
60	183.0
61	139.5
62	96.0
63	81.5
64	67.0
65	54.5
66	42.0
67	36.5
68	31.0
69	27.5
70	24.0
71	23.0
72	22.0
73	15.0
74	8.0
75	5.0
76	2.0
77	1.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38437303087586	27.925
2	12.728418399495906	10.100000000000001
3	5.293005671077505	6.3
4	3.5916824196597354	5.7
5	1.7013232514177694	3.375
6	1.1342155009451798	2.7
7	1.0081915563957151	2.8000000000000003
8	0.3780718336483932	1.2
9	0.3780718336483932	1.35
>10	2.9615626969124134	21.5
>50	0.2520478890989288	6.825
>100	0.1890359168241966	10.225
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	175	4.375	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	130	3.25	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	104	2.6	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	80	2.0	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	72	1.7999999999999998	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	66	1.6500000000000001	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	55	1.375	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	45	1.125	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	43	1.075	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	42	1.05	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	40	1.0	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	38	0.95	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	35	0.8750000000000001	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	32	0.8	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	30	0.75	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	26	0.65	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	22	0.5499999999999999	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	22	0.5499999999999999	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	20	0.5	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	18	0.44999999999999996	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	18	0.44999999999999996	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	17	0.42500000000000004	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	17	0.42500000000000004	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	16	0.4	No Hit
TTCTTCTCAAGCAGCTCACGAATCTGCTCCTTTAGGACCTCCACCTCCTC	16	0.4	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	16	0.4	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	15	0.375	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	15	0.375	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	15	0.375	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	15	0.375	No Hit
AGGATCGAGACGGCTTGTGATTTCTCTCACGAGACAGAGACCTTTCTCTT	15	0.375	No Hit
ATTCCAATCCACTGGCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGGC	15	0.375	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	14	0.35000000000000003	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	14	0.35000000000000003	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	13	0.325	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	13	0.325	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	13	0.325	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	13	0.325	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	13	0.325	No Hit
TTCTGCCATCAGCTGTCGTTTCATCAAACTTCTCTCCCAGGTTACAAGAG	12	0.3	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	12	0.3	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	12	0.3	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	12	0.3	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	11	0.27499999999999997	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	11	0.27499999999999997	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	11	0.27499999999999997	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	11	0.27499999999999997	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	11	0.27499999999999997	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTAT	11	0.27499999999999997	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	10	0.25	No Hit
CTGCCCGAGATGCTCATGTGCTCCTGCTCACTCAACATCTCTGGCCGTTC	10	0.25	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	10	0.25	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	10	0.25	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	10	0.25	No Hit
CCTGCTGGTGGTCTGGTCCTGTTCCTGCCCACCTGTGGTCGTAGCATCAG	9	0.22499999999999998	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	9	0.22499999999999998	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	9	0.22499999999999998	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	9	0.22499999999999998	No Hit
CACCCATGACCATGAGAAATCTGCTCAGCGAGGAGTAGAAGTAAAGGATG	9	0.22499999999999998	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	9	0.22499999999999998	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	8	0.2	No Hit
CTCCTTCTTCGAGACTTTCACAATTTTTATGCTGCTGCCCGAAGTCTTCT	8	0.2	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	8	0.2	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	8	0.2	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTC	8	0.2	No Hit
TGCTTGACTAAGTAGGTCTTCTCGTCGTTGAACTGCTCGTCCTCGGTCCT	8	0.2	No Hit
CCTTCTTCCATGGCCTGACTCTCGTGTTGTTTGCCAGCGGCTCCTTCTAC	7	0.17500000000000002	No Hit
TAGGAGTGCATGTCTCCAAAGTCCTTCTCGAAGAAGACGTAGGCCTTGCT	7	0.17500000000000002	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	7	0.17500000000000002	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	7	0.17500000000000002	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	7	0.17500000000000002	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	7	0.17500000000000002	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	7	0.17500000000000002	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	7	0.17500000000000002	No Hit
TCTTCCTCCTTCTCATCTTCTTCGTCCTCATCTTCATCTCTGTCATCATC	7	0.17500000000000002	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	7	0.17500000000000002	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	7	0.17500000000000002	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	7	0.17500000000000002	No Hit
GGTCTCTAGACAGCACTCTCTTCCTCTTCATGGTCCTCTCTTCCTCCAGC	7	0.17500000000000002	No Hit
TACTGTCATGGAGACCAGGTTCTCCCCTCGAAGCAACACCAGACCAAGGA	7	0.17500000000000002	No Hit
GCCAACTGCAACGACTAGACAGATTTTCTACAATAAGCCTGTACTCTGTA	7	0.17500000000000002	No Hit
CTGTTTCCTGCAGTCCAGGCGAGATTGACAGCAGTCTCCAACTTCTTGTT	7	0.17500000000000002	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	6	0.15	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	6	0.15	No Hit
GTGAACGCAGTGAAGGCCTCCTCCTCGTGCTTGGGTACCAAACGCCAATC	6	0.15	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	6	0.15	No Hit
AATCATCTTCCTGTGACCATTTCCAATCCTCCAGAGGCAGTCTCCAGTCT	6	0.15	No Hit
CTATGCTTTAGTGGGATTTCCTGCAGGTTTGGGATCGTAACCGTACAAGA	6	0.15	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	6	0.15	No Hit
ATGTAGGAGAGGCTTGTGAACATGTAACTGTTAACAGCCAACACCACGGA	6	0.15	No Hit
CTCAAGAGGTATGTTCCTATGAGCTAGCCTGAGCTTCCTTGCAATGCGCT	6	0.15	No Hit
CCTGGCTCAGTGCAGTTCCGCAAATGTAAGTCCTCCTTGGTGAAGCACAG	6	0.15	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	6	0.15	No Hit
TGAAAGGTCTTTGTCGAACACCTCTCCTGAGCGCAGCGCCGAGACCAGGT	6	0.15	No Hit
GAACGCCTTTACCAAACGGAGTGTTTCTTCCACACTGCGGCCCACCGGAA	6	0.15	No Hit
TTGTCAGACGCCTTCCAATGTAGATCCCCAAGCCAATGGCCAGCAGATGA	6	0.15	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	6	0.15	No Hit
CCTGAGAGACAGAGCAGGCCTGTTCTTCATGTACTTGTTACCCAGCCATA	6	0.15	No Hit
GTCCACCAGAGCTTCCTTGACTGTCTCCTGTCCGCACAGCACCACAATTC	6	0.15	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	6	0.15	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	5	0.125	No Hit
GTCCATGGTGTACACTTTCACTCCCCGGAAGCTGAAGCCCGAACACCGAT	5	0.125	No Hit
GCAAGCCGCACAGCCTTCTTGAGTTTCTCATGCCGGAAGATTTCCCTGCT	5	0.125	No Hit
ATGTGCACCTCCCCGAGAAGCAGATTCTCCCAAAATCCTTGCTCACTCAG	5	0.125	No Hit
GGTCCGTCTTGAAGTCCTGCGCGATCTCGCGCACCAGGCGCTGGAACGGC	5	0.125	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	5	0.125	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
TTGGCGCGGCACGTGTAGCTCAGGTTCCTCCGCACGCTGCGCTTGAAGAA	5	0.125	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACT	5	0.125	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	5	0.125	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	5	0.125	No Hit
TGGAGATCTGCTGCTCTACCATGGTGATCTCACGGTCCACGCGGTCCATG	5	0.125	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	5	0.125	No Hit
CTGAGACATCCCAGGAGAAGACACTGCATTCGGAGTCCCTCCATCCTGCT	5	0.125	No Hit
CATGTGGCGAAGACCTCACAAGCTTTTCAACGTAAGGGACAGGAATCATC	5	0.125	No Hit
CCCATTGCTCATAGTAGTTTCTCAAACTTTCTTCTGTGGTTTCAAAGCTT	5	0.125	No Hit
CCCAAGATCAACATACAGGTGATTTTCTCCATGCTCTTACCAGGCTCTCA	5	0.125	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	5	0.125	No Hit
GCAATCAGCAGAGTCAACACCAAGTCACCCAGAACAATCCCAGCCAGTAC	5	0.125	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	5	0.125	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	5	0.125	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	5	0.125	No Hit
GGCACGCATGCTTGTCCCACAGAGAGTCATCTGATCCAGTCATCACGCAG	5	0.125	No Hit
CTCACCTGTGCTCAGATCCACACCTACGAGCTGGCCGGATTCCGAATGTT	5	0.125	No Hit
TCTCCATCGCCGTCTTTGTCAAACTCCTCTATCATGGCCCGGAGCTCCTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTCA	20	6.952051E-4	44.000004	23
CAAGATC	20	6.952051E-4	44.000004	8
TTCACAC	20	6.952051E-4	44.000004	26
GGCACTT	20	6.952051E-4	44.000004	19
TGGCACT	20	6.952051E-4	44.000004	18
CACACTG	20	6.952051E-4	44.000004	28
ATCTCCT	20	6.952051E-4	44.000004	12
ACAGACA	20	6.952051E-4	44.000004	3
CTCCCGC	20	6.952051E-4	44.000004	42
CTCCTGG	20	6.952051E-4	44.000004	14
CCTGTCT	20	6.952051E-4	44.000004	16
TTTTCAC	20	6.952051E-4	44.000004	24
ACACTGG	20	6.952051E-4	44.000004	29
TTTCACA	20	6.952051E-4	44.000004	25
TTATACA	20	6.952051E-4	44.000004	4
ACTTTTC	20	6.952051E-4	44.000004	22
CCCGCTC	20	6.952051E-4	44.000004	44
CTGGCCC	20	6.952051E-4	44.000004	32
CTGGCAC	20	6.952051E-4	44.000004	17
GGCCCTT	20	6.952051E-4	44.000004	34
>>END_MODULE
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
Read 265813 spots for SRR25943839.sra
Written 265813 spots for SRR25943839.sra
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
Read 265808 spots for SRR25943839.sra
Written 265808 spots for SRR25943839.sra
SRR ids: ['SRR25943839.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_g1jd_em8
SRR25943839.sra spots: 2126469
blocks: [[1, 265808], [265809, 531616], [531617, 797424], [797425, 1063232], [1063233, 1329040], [1329041, 1594848], [1594849, 1860656], [1860657, 2126469]]
SRR25943839 file size 366888
SRR25943839 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943839 SRR25943839_1.fastq
Input file:	SRR25943839_1.fastq
trimmed:	SRR25943839-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:42:30 2026 >> started

Tue May  5 05:42:31 2026 >> done (0.833s)
2126469 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2126469 (100.00%) reads available; of these:
     47 ( 0.00%) trimmed reads available after processing
2126422 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      3	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	      7	  0.00%
 47	      7	  0.00%
 48	     10	  0.00%
 49	     18	  0.00%
 50	2126422	100.00%
2126469 reads passed initial QC


criterion=sequence-density
sequence-density=4.67
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=23
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCACCCT


criterion=fanout-score
sequence-density=2.90
sequence-density-rank=5
fanout-score=21.23
fanout-score-rank=1
prefix-density=3.21
prefix-fanout=19.2
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTCGTA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:42:48
                             Started mapping on |	May 05 05:42:48
                                    Finished on |	May 05 05:42:52
       Mapping speed, Million of reads per hour |	1913.82

                          Number of input reads |	2126469
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1750605
                        Uniquely mapped reads % |	82.32%
                          Average mapped length |	29.61
                       Number of splices: Total |	2801
            Number of splices: Annotated (sjdb) |	766
                       Number of splices: GT/AG |	2711
                       Number of splices: GC/AG |	36
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	54
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.05%
                        Deletion average length |	1.01
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	165164
             % of reads mapped to multiple loci |	7.77%
        Number of reads mapped to too many loci |	42998
             % of reads mapped to too many loci |	2.02%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.64%
                     % of reads unmapped: other |	0.24%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	210700	210700	210700
N_multimapping	165164	165164	165164
N_noFeature	11201	1683667	12193
N_ambiguous	80427	73	15032
UnstrandedReadsAssigned:1658977 PositiveStrandReadsAssigned:66865 NegativeStrandReadsAssigned:1723380
Dataset is classified negative stranded
MeadianReadLen=30 20thPercentileLength=30 echo kmer=25
SRR25943839 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=25

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 25
[index] number of targets: 109,282
[index] number of k-mers: 94,700,028
[index] number of equivalence classes: 457,473
[quant] running in single-end mode
[quant] will process file 1: SRR25943839-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,126,469 reads, 1,771,272 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,130 rounds

 109283 SRR25943839.ke.tsv
  52636 SRR25943839.se.tsv
 161919 total
==> SRR25943839.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943839.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943839 completed mapping pipeline successfully
