Starting /dee2/code/volunteer_pipeline.sh SRR25943835
    current disk space = 1264019436
    free memory = 196377656 
SRR25943835 SRAfilesize
4c24a9f5b4ef3689a4e1f3d2e6807df8  SRR25943835.sra
SRR25943835.sra file validated
SRR25943835 is single end
SRR25943835 is conventional basespace
SRR25943835 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3895	32.0	32.0	32.0	32.0	32.0
2	31.552	32.0	32.0	32.0	32.0	32.0
3	31.56725	32.0	32.0	32.0	32.0	32.0
4	31.57575	32.0	32.0	32.0	32.0	32.0
5	31.708	32.0	32.0	32.0	32.0	32.0
6	34.9725	36.0	36.0	36.0	36.0	36.0
7	35.199	36.0	36.0	36.0	36.0	36.0
8	34.84525	36.0	36.0	36.0	32.0	36.0
9	34.89775	36.0	36.0	36.0	32.0	36.0
10	34.98825	36.0	36.0	36.0	32.0	36.0
11	35.01525	36.0	36.0	36.0	36.0	36.0
12	35.04175	36.0	36.0	36.0	36.0	36.0
13	35.084	36.0	36.0	36.0	36.0	36.0
14	35.13125	36.0	36.0	36.0	36.0	36.0
15	35.03175	36.0	36.0	36.0	36.0	36.0
16	34.924	36.0	36.0	36.0	32.0	36.0
17	35.02225	36.0	36.0	36.0	36.0	36.0
18	34.971	36.0	36.0	36.0	36.0	36.0
19	35.071	36.0	36.0	36.0	36.0	36.0
20	35.10675	36.0	36.0	36.0	36.0	36.0
21	35.096	36.0	36.0	36.0	36.0	36.0
22	34.901	36.0	36.0	36.0	36.0	36.0
23	35.01875	36.0	36.0	36.0	36.0	36.0
24	34.93125	36.0	36.0	36.0	32.0	36.0
25	35.129	36.0	36.0	36.0	36.0	36.0
26	34.83575	36.0	36.0	36.0	32.0	36.0
27	34.94025	36.0	36.0	36.0	36.0	36.0
28	34.83275	36.0	36.0	36.0	32.0	36.0
29	34.83825	36.0	36.0	36.0	32.0	36.0
30	34.79775	36.0	36.0	36.0	32.0	36.0
31	35.0735	36.0	36.0	36.0	36.0	36.0
32	34.92675	36.0	36.0	36.0	32.0	36.0
33	34.742	36.0	36.0	36.0	32.0	36.0
34	34.87475	36.0	36.0	36.0	32.0	36.0
35	34.8995	36.0	36.0	36.0	32.0	36.0
36	34.912	36.0	36.0	36.0	32.0	36.0
37	34.76625	36.0	36.0	36.0	32.0	36.0
38	34.66	36.0	36.0	36.0	32.0	36.0
39	34.728	36.0	36.0	36.0	32.0	36.0
40	34.526	36.0	36.0	36.0	32.0	36.0
41	34.666	36.0	36.0	36.0	32.0	36.0
42	34.6535	36.0	36.0	36.0	32.0	36.0
43	34.199	36.0	36.0	36.0	32.0	36.0
44	34.44575	36.0	36.0	36.0	32.0	36.0
45	34.3005	36.0	36.0	36.0	32.0	36.0
46	34.09175	36.0	36.0	36.0	32.0	36.0
47	33.9335	36.0	36.0	36.0	32.0	36.0
48	33.9085	36.0	36.0	36.0	32.0	36.0
49	34.116	36.0	36.0	36.0	32.0	36.0
50	33.5845	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
11101	1	-0.3670033670033668
11101	2	1.0572390572390553
11101	3	-0.21212121212121104
11101	4	-0.30976430976431146
11101	5	0.7230639730639723
11101	6	-0.07744107744107254
11101	7	1.0580808080808097
11101	8	-0.21380471380471278
11101	9	-0.07575757575757791
11101	10	1.481481481481481
11101	11	2.874579124579121
11101	12	-0.22390572390572316
11101	13	-0.4208754208754186
11101	14	-0.22727272727272663
11101	15	-0.3484848484848513
11101	16	1.1843434343434325
11101	17	1.8451178451178478
11101	18	-0.32996632996633224
11101	19	1.3905723905723946
11101	20	-0.12457912457912812
11101	21	-0.5892255892255918
11101	22	1.41245791245791
11101	23	-0.15824915824916275
11101	24	-0.41245791245790997
11101	25	-0.057239057239058866
11101	26	-0.5033670033670035
11101	27	0.05218855218855367
11101	28	-0.5168350168350173
11101	29	-0.8164983164983184
11101	30	1.8720538720538755
11101	31	-0.4579124579124567
11101	32	-0.4023569023568996
11101	33	1.993265993265993
11101	34	-0.04040404040404155
11101	35	0.33417508417508657
11101	36	0.10437710437710734
11101	37	1.4292929292929273
11101	38	-0.39562289562289266
11101	39	1.6902356902356885
11101	40	0.021885521885515402
11101	41	1.2592592592592595
11101	42	-0.5757575757575779
11101	43	-0.10101010101010388
11101	44	-0.2104377104377093
11101	45	-0.39393939393939803
11101	46	2.7297979797979792
11101	47	3.1599326599326574
11101	48	1.8585858585858546
11101	49	0.0269360269360277
11101	50	-0.5892255892255918
11102	1	-0.05387205387205185
11102	2	1.602693602693602
11102	3	-0.14141414141414188
11102	4	-0.03703703703703809
11102	5	0.9402356902356885
11102	6	0.11952861952862293
11102	7	1.037878787878789
11102	8	-0.7138047138047128
11102	9	-0.6010101010101039
11102	10	1.562289562289564
11102	11	2.632154882154879
11102	12	0.20033670033669893
11102	13	-0.40067340067339785
11102	14	-0.343434343434339
11102	15	-0.22727272727273373
11102	16	0.6338383838383805
11102	17	1.26936026936027
11102	18	-0.3602693602693634
11102	19	1.8299663299663322
11102	20	0.12794612794612448
11102	21	-0.07407407407407618
11102	22	1.4730639730639723
11102	23	-0.3804713804713842
11102	24	-0.34680134680134955
11102	25	-0.5673400673400693
11102	26	-0.28114478114478203
11102	27	0.05218855218855367
11102	28	-0.031986531986532896
11102	29	-0.20538720538720412
11102	30	1.094276094276097
11102	31	-0.0033670033670034627
11102	32	-0.34680134680134955
11102	33	0.8417508417508373
11102	34	0.1616161616161591
11102	35	0.35437710437710734
11102	36	0.0589225589225606
11102	37	1.5303030303030312
11102	38	-0.41077441077440824
11102	39	1.149831649831647
11102	40	-0.16498316498316967
11102	41	1.6026936026935985
11102	42	-0.14646464646465063
11102	43	-0.13636363636364024
11102	44	-0.7104377104377093
11102	45	-0.02525252525252597
11102	46	2.361111111111107
11102	47	2.968013468013467
11102	48	1.419191919191917
11102	49	-1.23063973063973
11102	50	-0.9276094276094256
11103	1	0.4208754208754222
11103	2	-2.659932659932661
11103	3	0.3535353535353529
11103	4	0.346801346801346
11103	5	-1.6632996632996644
11103	6	-0.04208754208753618
11103	7	-2.0959595959595916
11103	8	0.9276094276094256
11103	9	0.6767676767676747
11103	10	-3.043771043771045
11103	11	-5.506734006734007
11103	12	0.02356902356902424
11103	13	0.8215488215488236
11103	14	0.5707070707070727
11103	15	0.5757575757575708
11103	16	-1.8181818181818201
11103	17	-3.1144781144781106
11103	18	0.6902356902356885
11103	19	-3.2205387205387197
11103	20	-0.0033670033670034627
11103	21	0.6632996632996608
11103	22	-2.8855218855218894
11103	23	0.5387205387205327
11103	24	0.7592592592592595
11103	25	0.624579124579121
11103	26	0.7845117845117855
11103	27	-0.10437710437710024
11103	28	0.5488215488215502
11103	29	1.0218855218855225
11103	30	-2.9663299663299654
11103	31	0.4612794612794602
11103	32	0.7491582491582491
11103	33	-2.8350168350168374
11103	34	-0.12121212121212466
11103	35	-0.6885521885521868
11103	36	-0.16329966329966084
11103	37	-2.9595959595959584
11103	38	0.806397306397308
11103	39	-2.8400673400673426
11103	40	0.14309764309764006
11103	41	-2.861952861952865
11103	42	0.7222222222222214
11103	43	0.23737373737373701
11103	44	0.9208754208754186
11103	45	0.4191919191919169
11103	46	-5.0909090909090935
11103	47	-6.127946127946132
11103	48	-3.2777777777777786
11103	49	1.2037037037037024
11103	50	1.5168350168350173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	4.0
25	7.0
26	13.0
27	16.0
28	56.0
29	67.0
30	105.0
31	129.0
32	216.0
33	340.0
34	626.0
35	2420.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.849999999999998	25.55	24.125	26.474999999999998
2	19.6	20.225	26.950000000000003	33.225
3	21.675	25.0	21.8	31.525
4	19.75	22.95	25.775	31.525
5	16.125	28.749999999999996	24.025	31.1
6	26.64498373780335	26.745058794095574	23.817863397548162	22.792094070552913
7	24.075	19.175	29.825000000000003	26.924999999999997
8	19.175	21.3	24.9	34.625
9	25.1	17.150000000000002	22.425	35.325
10	25.874999999999996	20.7	23.150000000000002	30.275000000000002
11	28.449999999999996	14.899999999999999	23.375	33.275
12	28.4	17.575	24.3	29.725
13	28.475	21.95	26.0	23.575
14	22.6	20.974999999999998	27.35	29.075
15	25.124999999999996	21.875	20.175	32.824999999999996
16	24.6	19.725	25.7	29.975
17	19.425	20.025000000000002	27.875	32.675
18	28.249999999999996	18.224999999999998	30.075000000000003	23.45
19	26.674999999999997	16.375	28.975	27.975
20	24.75	17.150000000000002	26.625	31.474999999999998
21	27.075	19.475	19.725	33.725
22	17.825	20.75	36.725	24.7
23	30.025000000000002	12.4	21.525	36.05
24	23.25	12.075	22.35	42.325
25	3.925	7.425	71.475	17.175
26	20.575	15.45	25.25	38.725
27	27.35	19.75	26.974999999999998	25.924999999999997
28	17.549999999999997	19.175	29.099999999999998	34.175
29	30.45	21.275	20.45	27.825
30	25.324999999999996	20.95	22.6	31.125000000000004
31	23.35	26.474999999999998	25.3	24.875
32	24.825	22.425	21.2	31.55
33	32.300000000000004	19.675	18.525	29.5
34	28.225	20.599999999999998	28.599999999999998	22.575
35	27.575	17.05	25.525	29.849999999999998
36	24.55	15.925	20.8	38.725
37	19.675	20.724999999999998	28.875	30.725
38	30.25	13.600000000000001	23.7	32.45
39	27.800000000000004	15.174999999999999	23.875	33.15
40	21.349999999999998	22.7	31.724999999999998	24.224999999999998
41	24.3	20.65	18.65	36.4
42	24.61230615307654	23.386693346673336	18.659329664832416	33.34167083541771
43	25.624999999999996	27.35	26.1	20.925
44	24.4	20.424999999999997	23.200000000000003	31.974999999999998
45	18.104526131532882	22.05551387846962	22.80570142535634	37.034258564641156
46	21.95	23.175	31.025000000000002	23.849999999999998
47	24.5	18.125	26.3	31.075000000000003
48	23.686843421710854	23.686843421710854	19.759879939969984	32.86643321660831
49	21.099999999999998	19.55	35.949999999999996	23.400000000000002
50	21.8	12.675	20.974999999999998	44.55
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	1.0
32	2.0
33	1.5
34	1.0
35	2.0
36	3.0
37	6.5
38	10.0
39	19.5
40	29.0
41	54.5
42	80.0
43	73.5
44	67.0
45	130.5
46	194.0
47	248.5
48	303.0
49	408.5
50	514.0
51	562.5
52	611.0
53	487.0
54	363.0
55	459.5
56	556.0
57	582.0
58	608.0
59	404.0
60	200.0
61	171.5
62	143.0
63	117.0
64	91.0
65	91.0
66	91.0
67	62.5
68	34.0
69	37.0
70	40.0
71	46.5
72	53.0
73	28.5
74	4.0
75	2.0
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.05
43	0.0
44	0.0
45	0.025
46	0.0
47	0.0
48	0.05
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.150000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.71980074719801	30.0
2	9.028642590286426	7.249999999999999
3	4.35865504358655	5.25
4	2.86425902864259	4.6
5	1.7434620174346203	3.5000000000000004
6	1.432129514321295	3.45
7	1.1207970112079702	3.15
8	0.9962640099626401	3.2
9	0.24906600249066002	0.8999999999999999
>10	3.1755915317559156	28.7
>50	0.24906600249066002	6.75
>100	0.062266500622665005	3.25
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	130	3.25	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	79	1.975	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	71	1.775	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	64	1.6	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	56	1.4000000000000001	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	48	1.2	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	47	1.175	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	42	1.05	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	42	1.05	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	42	1.05	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	40	1.0	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	40	1.0	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCAT	40	1.0	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	40	1.0	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	36	0.8999999999999999	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	34	0.8500000000000001	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	31	0.775	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	30	0.75	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	29	0.7250000000000001	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	28	0.7000000000000001	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	27	0.675	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	27	0.675	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	25	0.625	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	24	0.6	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	23	0.575	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	23	0.575	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	21	0.525	No Hit
CCTTCTTCCATGGCCTGACTCTCGTGTTGTTTGCCAGCGGCTCCTTCTAC	20	0.5	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	19	0.475	No Hit
GTCCACCAGAGCTTCCTTGACTGTCTCCTGTCCGCACAGCACCACAATTC	19	0.475	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	19	0.475	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	18	0.44999999999999996	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	18	0.44999999999999996	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	17	0.42500000000000004	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	17	0.42500000000000004	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	16	0.4	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTT	15	0.375	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	15	0.375	No Hit
GCAGGGTGACCAGCTTTCCTTCCTTGATGGCAGCATGGAAGCTGTGGGCG	15	0.375	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	14	0.35000000000000003	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	14	0.35000000000000003	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	14	0.35000000000000003	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	14	0.35000000000000003	No Hit
AATCATCTTCCTGTGACCATTTCCAATCCTCCAGAGGCAGTCTCCAGTCT	13	0.325	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	13	0.325	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	12	0.3	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	12	0.3	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	11	0.27499999999999997	No Hit
TCTCTGGTCCAGCTGTGGTGGAGCTGGTGAAGGTGAGCAAGCAGAGCGCC	11	0.27499999999999997	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	11	0.27499999999999997	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	11	0.27499999999999997	No Hit
AGGATCGAGACGGCTTGTGATTTCTCTCACGAGACAGAGACCTTTCTCTT	11	0.27499999999999997	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	10	0.25	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	10	0.25	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	10	0.25	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	10	0.25	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	9	0.22499999999999998	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	9	0.22499999999999998	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	9	0.22499999999999998	No Hit
TCAGGAGGAACTTGGTCTCTGTGGTCACATTGTGCATGACCTGCCAGTGC	8	0.2	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	8	0.2	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	8	0.2	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	8	0.2	No Hit
CTGCCCGAGATGCTCATGTGCTCCTGCTCACTCAACATCTCTGGCCGTTC	8	0.2	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	8	0.2	No Hit
GAATCTTCTCCTGGGAGCAGCTCCTCCCAGAGAGTCTCTCCGGATGCTCC	8	0.2	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCT	8	0.2	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	8	0.2	No Hit
CTGGTTCCGCAGGAACTTGTCCTCTAGCACCTGCAGGCACCCATCCAGTT	8	0.2	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	8	0.2	No Hit
TTGTCAGACGCCTTCCAATGTAGATCCCCAAGCCAATGGCCAGCAGATGA	8	0.2	No Hit
GTTGCTAAGCTTCCAACCAAACTCTCCTGCTCCTCAATTTCCTGGTATTC	8	0.2	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	8	0.2	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	7	0.17500000000000002	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	7	0.17500000000000002	No Hit
ACACCGCTTTCATCGCCATGCTTCCCCGGGAGACGCGCGGCGGCCTCCTC	7	0.17500000000000002	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	7	0.17500000000000002	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	7	0.17500000000000002	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	7	0.17500000000000002	No Hit
TAGTAATATCCTGAGTCCTCCACCTTGGCAGGTACAAACCAAAGATGTTC	7	0.17500000000000002	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	7	0.17500000000000002	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	7	0.17500000000000002	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	7	0.17500000000000002	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	7	0.17500000000000002	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	7	0.17500000000000002	No Hit
TTCTTCTCAAGCAGCTCACGAATCTGCTCCTTTAGGACCTCCACCTCCTC	7	0.17500000000000002	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	7	0.17500000000000002	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	7	0.17500000000000002	No Hit
ATTCCAATCCACTGGCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGGC	7	0.17500000000000002	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	7	0.17500000000000002	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	7	0.17500000000000002	No Hit
TACTTAGTGACAGCCTTGGTGCCCTCCGACACGGCGTGCTTGGCCAGCTC	6	0.15	No Hit
TTCTTAGTGGGCAGACCCAGCTGGCCTTCAGACTGCTTGCTGTTCCTGGC	6	0.15	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	6	0.15	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	6	0.15	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	6	0.15	No Hit
AACAGCTCCTTGAGAACACGGTCCTGACTCTGCACGGTGTGCACGATGAT	6	0.15	No Hit
CCAGTACCAGCTACTCCGCTCCTCCGAGTCGAAGTTGCCCGCCATGGCCG	6	0.15	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	6	0.15	No Hit
GTCAACCTCATGTGGCAGGTTCCCAATGAAGAGCTGGTGACTGTCAGGGT	6	0.15	No Hit
TATGTCCACCAGATCGCTGTGGCCCAGGACATCCACAATACCCTCTGTAA	6	0.15	No Hit
CAGGTTGAGGGCAACTAGTTTTTCTCCGTGACTGTTGGGTAGTGGATCAT	6	0.15	No Hit
TCCTTAGCCTCCAGGGTCTGAGCAGGGGATCGGATCCCAGACCGGTCCAA	6	0.15	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	6	0.15	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	6	0.15	No Hit
TTGGAGAAGACCTGCTCCAGCGCCTGCTCGTTGGTGTCGAAGCTGAGTCC	6	0.15	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	6	0.15	No Hit
TGTCTGGCTTTCAGGCTCAGGCACCTTCCAGTCCATCTTTCGGCCCTTCT	6	0.15	No Hit
CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTA	6	0.15	No Hit
TCTTGACGGTGTCCGGATTCGATCCAGAGAAGCCGAACTCAGAAAGCGTT	6	0.15	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	6	0.15	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	6	0.15	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	6	0.15	No Hit
ATACTGGCACCGCGGTTGGCAACACCGGCAGAAAAGTCGTTGATGTTGGA	6	0.15	No Hit
GTCACATCCAGTGCCTCCGGTGAGCCAGGCTGCTGGACAGACGTGGTCGG	5	0.125	No Hit
GACCATTTCTCCTCGCTGAGCTGGCAGCACGTGGAGGCAATGCTCACCAT	5	0.125	No Hit
AGCTCTTGGTGATGATGGCCCGGCCCCCAAGGTGGCGAGGCTCCAGTGCT	5	0.125	No Hit
CCTGCTGGTGGTCTGGTCCTGTTCCTGCCCACCTGTGGTCGTAGCATCAG	5	0.125	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	5	0.125	No Hit
GAGAGCAAGACTTCTGAACTTTACTGACGACACGCGTCTTCATAGGCTTC	5	0.125	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	5	0.125	No Hit
TTGTCCACGTAGCGGCGGTATCGTTCCTCCATGGCCCGCAGGTCTGCATC	5	0.125	No Hit
AGCACCTTCTTCACATTCACCACGCCGTAGAGTGCTGCCGTCACGTGCTG	5	0.125	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	5	0.125	No Hit
TTGTCTGACGAGTCAGGCATTTGGTCCACCAAATGCCGGTCCTCAGAGTT	5	0.125	No Hit
CCACTGCCCACGATGCCCACGCAGACTCCACAGAACAGGTTGGACAGACC	5	0.125	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	5	0.125	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
CCCTCCCAGTGTCAAACCGAGGTTTGGTCTTCACAGCAGTGACAGTGGTG	5	0.125	No Hit
CGGCGCCAATTCCTTTCACCGAGTTCGCGAGGCAAGACGCGGAGACTCGC	5	0.125	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	5	0.125	No Hit
GGACAATCCTCCTCAGCAGCCTGCTGCTTACAGCAGATGGCGTCAACACA	5	0.125	No Hit
CAGCTGCTGCCCGCCGACATGTCTCCTGCACGCCGCCCACGACCCTCCAG	5	0.125	No Hit
ACTCTCGGATCCCAAGATGTCCGCCCGCCCCCAGCTCGCCTGTCTGCTGC	5	0.125	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	5	0.125	No Hit
TCACTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATC	5	0.125	No Hit
GAACGCCTTTACCAAACGGAGTGTTTCTTCCACACTGCGGCCCACCGGAA	5	0.125	No Hit
AGCTCGTCCTCTGTGGTCTTCTGGTAGACTGGGTTGTCAAAGTTTATGCT	5	0.125	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	5	0.125	No Hit
AGCACATGGCCCTGACCAGTACACTTTGCACTGGCACAGCCTGATGGCAT	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
ATCCTGCCCACACCTAGGTTGCTCTCCACAATGCTGACAGAATGCCTCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
Read 213223 spots for SRR25943835.sra
Written 213223 spots for SRR25943835.sra
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
Read 213221 spots for SRR25943835.sra
Written 213221 spots for SRR25943835.sra
SRR ids: ['SRR25943835.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_rg_r7_43
SRR25943835.sra spots: 1705770
blocks: [[1, 213221], [213222, 426442], [426443, 639663], [639664, 852884], [852885, 1066105], [1066106, 1279326], [1279327, 1492547], [1492548, 1705770]]
SRR25943835 file size 294088
SRR25943835 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943835 SRR25943835_1.fastq
Input file:	SRR25943835_1.fastq
trimmed:	SRR25943835-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:41:46 2026 >> started

Tue May  5 05:41:47 2026 >> done (0.779s)
1705770 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
1705770 (100.00%) reads available; of these:
     34 ( 0.00%) trimmed reads available after processing
1705736 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      9	  0.00%
 47	      4	  0.00%
 48	      5	  0.00%
 49	     15	  0.00%
 50	1705736	100.00%
1705770 reads passed initial QC


criterion=sequence-density
sequence-density=8.62
sequence-density-rank=1
fanout-score=14.64
fanout-score-rank=1
prefix-density=8.97
prefix-fanout=14.1
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC


criterion=fanout-score
sequence-density=8.62
sequence-density-rank=1
fanout-score=14.64
fanout-score-rank=1
prefix-density=8.97
prefix-fanout=14.1
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC -o SRR25943835 -
Input file:	STDIN
trimmed:	SRR25943835-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Tue May  5 05:41:50 2026 >> started

Tue May  5 05:41:50 2026 >> done (0.537s)
1326710 reads processed; of these:
  71260 ( 5.37%) short reads filtered out after trimming by size control
   4223 ( 0.32%) empty reads filtered out after trimming by size control
1251227 (94.31%) reads available; of these:
 103939 ( 8.31%) trimmed reads available after processing
1147288 (91.69%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    327	  0.03%
 19	    469	  0.04%
 20	    704	  0.06%
 21	    521	  0.04%
 22	    756	  0.06%
 23	   1077	  0.09%
 24	   1917	  0.15%
 25	   3076	  0.25%
 26	   2325	  0.19%
 27	   3122	  0.25%
 28	   2737	  0.22%
 29	   2761	  0.22%
 30	   2704	  0.22%
 31	   3930	  0.31%
 32	   2833	  0.23%
 33	   2909	  0.23%
 34	   2738	  0.22%
 35	   2484	  0.20%
 36	   2334	  0.19%
 37	   2376	  0.19%
 38	   3045	  0.24%
 39	   3657	  0.29%
 40	   2396	  0.19%
 41	   2344	  0.19%
 42	   3280	  0.26%
 43	   2410	  0.19%
 44	   3470	  0.28%
 45	   4097	  0.33%
 46	  17547	  1.40%
 47	  19604	  1.57%
 48	      4	  0.00%
 49	     10	  0.00%
 50	1147263	 91.69%


criterion=sequence-density
sequence-density=3.86
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTTCC


criterion=fanout-score
sequence-density=2.01
sequence-density-rank=3
fanout-score=14.84
fanout-score-rank=1
prefix-density=2.16
prefix-fanout=13.8
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
                                 Started job on |	May 05 05:41:56
                             Started mapping on |	May 05 05:41:56
                                    Finished on |	May 05 05:41:59
       Mapping speed, Million of reads per hour |	1956.34

                          Number of input reads |	1630287
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1405534
                        Uniquely mapped reads % |	86.21%
                          Average mapped length |	48.91
                       Number of splices: Total |	2502
            Number of splices: Annotated (sjdb) |	1491
                       Number of splices: GT/AG |	1986
                       Number of splices: GC/AG |	128
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	388
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.12%
                        Deletion average length |	1.41
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	112469
             % of reads mapped to multiple loci |	6.90%
        Number of reads mapped to too many loci |	3031
             % of reads mapped to too many loci |	0.19%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.69%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	112284	112284	112284
N_multimapping	112469	112469	112469
N_noFeature	2043	1320006	2268
N_ambiguous	97366	42	12826
UnstrandedReadsAssigned:1306125 PositiveStrandReadsAssigned:85486 NegativeStrandReadsAssigned:1390440
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943835 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943835-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,630,287 reads, 1,378,912 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,121 rounds

 109283 SRR25943835.ke.tsv
  52636 SRR25943835.se.tsv
 161919 total
==> SRR25943835.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943835.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943835 completed mapping pipeline successfully
