Starting /dee2/code/volunteer_pipeline.sh SRR25943848
    current disk space = 1265791880
    free memory = 192789220 
SRR25943848 SRAfilesize
bf0dbc2e43c46386b2f84eeecfe3c5b4  SRR25943848.sra
SRR25943848.sra file validated
SRR25943848 is single end
SRR25943848 is conventional basespace
SRR25943848 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943848_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25	32.0	32.0	32.0	32.0	32.0
2	31.4315	32.0	32.0	32.0	32.0	32.0
3	31.49375	32.0	32.0	32.0	32.0	32.0
4	31.51075	32.0	32.0	32.0	32.0	32.0
5	31.5195	32.0	32.0	32.0	32.0	32.0
6	35.0265	36.0	36.0	36.0	36.0	36.0
7	34.9795	36.0	36.0	36.0	36.0	36.0
8	34.91075	36.0	36.0	36.0	32.0	36.0
9	34.9405	36.0	36.0	36.0	32.0	36.0
10	34.928	36.0	36.0	36.0	32.0	36.0
11	34.91275	36.0	36.0	36.0	32.0	36.0
12	35.07575	36.0	36.0	36.0	36.0	36.0
13	34.9885	36.0	36.0	36.0	36.0	36.0
14	34.94125	36.0	36.0	36.0	36.0	36.0
15	34.79875	36.0	36.0	36.0	32.0	36.0
16	34.904	36.0	36.0	36.0	32.0	36.0
17	34.85575	36.0	36.0	36.0	32.0	36.0
18	34.77725	36.0	36.0	36.0	32.0	36.0
19	34.96725	36.0	36.0	36.0	36.0	36.0
20	34.92425	36.0	36.0	36.0	36.0	36.0
21	34.967	36.0	36.0	36.0	36.0	36.0
22	34.918	36.0	36.0	36.0	32.0	36.0
23	34.97275	36.0	36.0	36.0	36.0	36.0
24	34.99525	36.0	36.0	36.0	36.0	36.0
25	35.09225	36.0	36.0	36.0	36.0	36.0
26	34.7845	36.0	36.0	36.0	32.0	36.0
27	34.807	36.0	36.0	36.0	32.0	36.0
28	34.69575	36.0	36.0	36.0	32.0	36.0
29	34.83325	36.0	36.0	36.0	32.0	36.0
30	34.784	36.0	36.0	36.0	32.0	36.0
31	34.819	36.0	36.0	36.0	32.0	36.0
32	34.70475	36.0	36.0	36.0	32.0	36.0
33	34.8245	36.0	36.0	36.0	32.0	36.0
34	34.77925	36.0	36.0	36.0	32.0	36.0
35	35.017	36.0	36.0	36.0	36.0	36.0
36	34.833	36.0	36.0	36.0	36.0	36.0
37	34.915	36.0	36.0	36.0	36.0	36.0
38	34.83725	36.0	36.0	36.0	32.0	36.0
39	34.84475	36.0	36.0	36.0	32.0	36.0
40	34.48975	36.0	36.0	36.0	32.0	36.0
41	34.581	36.0	36.0	36.0	32.0	36.0
42	34.7255	36.0	36.0	36.0	32.0	36.0
43	34.42975	36.0	36.0	36.0	32.0	36.0
44	34.444	36.0	36.0	36.0	32.0	36.0
45	34.525	36.0	36.0	36.0	32.0	36.0
46	34.50625	36.0	36.0	36.0	32.0	36.0
47	34.363	36.0	36.0	36.0	32.0	36.0
48	34.3485	36.0	36.0	36.0	32.0	36.0
49	34.46325	36.0	36.0	36.0	32.0	36.0
50	34.22525	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	3.0
25	5.0
26	19.0
27	25.0
28	50.0
29	71.0
30	96.0
31	156.0
32	191.0
33	312.0
34	608.0
35	2461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.349999999999998	17.549999999999997	23.474999999999998	30.625000000000004
2	27.750000000000004	18.05	32.175	22.025
3	42.55	23.125	16.775000000000002	17.549999999999997
4	38.45	20.125	17.05	24.375
5	15.575	31.424999999999997	27.075	25.924999999999997
6	36.343171585792895	20.460230115057527	23.23661830915458	19.959979989995
7	38.05	15.275	30.475	16.2
8	17.875	16.400000000000002	20.0	45.725
9	17.625	18.925	35.075	28.375
10	26.55	14.35	25.5	33.6
11	25.45	38.625	14.499999999999998	21.425
12	41.625	19.475	15.625	23.275000000000002
13	39.875	25.35	15.024999999999999	19.75
14	16.75	20.625	24.25	38.375
15	15.625	28.000000000000004	20.45	35.925000000000004
16	14.725	37.95	23.0	24.325
17	16.675	21.925	31.724999999999998	29.675
18	17.0	15.4	43.0	24.6
19	18.175	23.575	27.3	30.95
20	29.599999999999998	13.0	28.599999999999998	28.799999999999997
21	15.65	20.275000000000002	36.199999999999996	27.875
22	16.2	18.15	33.775	31.874999999999996
23	29.325000000000003	22.625	22.475	25.575
24	23.7	14.95	28.349999999999998	33.0
25	1.0999999999999999	3.675	85.075	10.15
26	11.924999999999999	21.65	17.325	49.1
27	27.725	21.9	28.675	21.7
28	16.275000000000002	21.725	26.0	36.0
29	40.875	23.025000000000002	11.924999999999999	24.175
30	32.225	16.85	21.25	29.675
31	38.175	23.35	12.275	26.200000000000003
32	24.2	19.225	20.974999999999998	35.6
33	28.975	20.549999999999997	13.900000000000002	36.575
34	23.425	18.525	36.025	22.025
35	31.0	20.3	28.225	20.474999999999998
36	40.775	10.925	27.175	21.125
37	23.674999999999997	38.175	21.65	16.5
38	38.1	11.175	16.900000000000002	33.825
39	37.55	24.625	15.8	22.025
40	21.125	24.5	19.3	35.075
41	17.175	34.599999999999994	16.950000000000003	31.275
42	38.95	16.2	24.0	20.849999999999998
43	27.250000000000004	21.975	17.825	32.95
44	31.825	14.149999999999999	23.549999999999997	30.475
45	12.2	34.075	14.299999999999999	39.425
46	15.975	26.450000000000003	38.975	18.6
47	26.224999999999998	14.6	14.299999999999999	44.875
48	19.004751187796952	21.030257564391096	35.95898974743686	24.006001500375092
49	36.575	20.075000000000003	21.925	21.425
50	15.725	22.1	26.625	35.55
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	3.0
30	6.0
31	3.0
32	0.0
33	0.5
34	1.0
35	4.0
36	7.0
37	8.5
38	10.0
39	35.0
40	60.0
41	109.0
42	158.0
43	191.5
44	225.0
45	204.5
46	184.0
47	169.5
48	155.0
49	394.0
50	633.0
51	565.5
52	498.0
53	375.5
54	253.0
55	329.5
56	406.0
57	355.5
58	305.0
59	481.0
60	657.0
61	454.5
62	252.0
63	163.5
64	75.0
65	69.5
66	64.0
67	41.5
68	19.0
69	17.5
70	16.0
71	15.5
72	15.0
73	8.0
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.025
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6829268292683	22.35
2	11.869918699186991	7.3
3	4.5528455284552845	4.2
4	2.682926829268293	3.3000000000000003
5	1.5447154471544715	2.375
6	0.8130081300813009	1.5
7	0.894308943089431	1.925
8	0.6504065040650406	1.6
9	0.7317073170731708	2.025
>10	2.8455284552845526	18.975
>50	0.40650406504065045	7.9
>100	0.24390243902439024	13.25
>500	0.08130081300813008	13.3
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	532	13.3	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	208	5.2	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	164	4.1000000000000005	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	158	3.95	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	82	2.0500000000000003	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	65	1.625	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	62	1.55	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	55	1.375	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	52	1.3	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	50	1.25	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	42	1.05	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	38	0.95	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	37	0.9249999999999999	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	35	0.8750000000000001	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	34	0.8500000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	32	0.8	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	27	0.675	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	26	0.65	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	25	0.625	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	24	0.6	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	23	0.575	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	23	0.575	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	22	0.5499999999999999	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	22	0.5499999999999999	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	21	0.525	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	21	0.525	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	19	0.475	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	19	0.475	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	19	0.475	No Hit
CACCCATGACCATGAGAAATCTGCTCAGCGAGGAGTAGAAGTAAAGGATG	18	0.44999999999999996	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	18	0.44999999999999996	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	17	0.42500000000000004	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	17	0.42500000000000004	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	16	0.4	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	15	0.375	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	14	0.35000000000000003	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	12	0.3	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	11	0.27499999999999997	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	11	0.27499999999999997	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	11	0.27499999999999997	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	10	0.25	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	10	0.25	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	10	0.25	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	10	0.25	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	9	0.22499999999999998	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
AAACGCCCACTTTGACATAGACTTTATAGACCATACCACCAATACCGAAA	9	0.22499999999999998	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	9	0.22499999999999998	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAAT	9	0.22499999999999998	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	9	0.22499999999999998	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	9	0.22499999999999998	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	9	0.22499999999999998	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	9	0.22499999999999998	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	8	0.2	No Hit
CATCAGAAGACTCGGACACCAGCTTCCCATCTCGGGTTTCAATCTTCTTC	8	0.2	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	8	0.2	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	8	0.2	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	8	0.2	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	8	0.2	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	8	0.2	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	8	0.2	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	7	0.17500000000000002	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	7	0.17500000000000002	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	7	0.17500000000000002	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	7	0.17500000000000002	No Hit
TCTTCCACACTGATCACAGAGACCTTGGAGGTCAGGTCCACGCCCAGGTC	7	0.17500000000000002	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	7	0.17500000000000002	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	7	0.17500000000000002	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	7	0.17500000000000002	No Hit
TTGGCACTGTCAAAGGTCATTTGGTACCCAGCAAGCCAGCCCTCGTAACC	7	0.17500000000000002	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	7	0.17500000000000002	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	7	0.17500000000000002	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	6	0.15	No Hit
TGGCACAGACACATGTCCCGATCCTGTCACAAGAGGTAGAATTCCAGCGT	6	0.15	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	6	0.15	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	6	0.15	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	6	0.15	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	6	0.15	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	6	0.15	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	6	0.15	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	6	0.15	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	6	0.15	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	5	0.125	No Hit
CTTGCGGATGCCATCTTCTTCCACTTCGTAGTAGTCAGGCCCACAGGCTC	5	0.125	No Hit
GAAACTCATATCCCAAGTACAGACTGATGTCTATCTTTCGAGGGACCCTT	5	0.125	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	5	0.125	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	5	0.125	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	5	0.125	No Hit
GAACGCCTTTACCAAACGGAGTGTTTCTTCCACACTGCGGCCCACCGGAA	5	0.125	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	5	0.125	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	5	0.125	No Hit
AGAGTTCTGGCAAACTCACAACGTTCATAGACCTTGGCCTGAGCAGTGAC	5	0.125	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	5	0.125	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	5	0.125	No Hit
GCAATCAGCAGAGTCAACACCAAGTCACCCAGAACAATCCCAGCCAGTAC	5	0.125	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	5	0.125	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	5	0.125	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	5	0.125	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	5	0.125	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	5	0.125	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGAA	20	6.952051E-4	44.000004	6
GTTGAAA	20	6.952051E-4	44.000004	7
GCCTTAC	20	6.952051E-4	44.000004	32
GAAACAC	20	6.952051E-4	44.000004	10
CCTGTTG	20	6.952051E-4	44.000004	4
TGGTAAT	20	6.952051E-4	44.000004	22
AAACACA	20	6.952051E-4	44.000004	11
TACGGCC	20	6.952051E-4	44.000004	28
GAATGCC	20	6.952051E-4	44.000004	39
GGCCTTA	20	6.952051E-4	44.000004	31
ATACGGC	20	6.952051E-4	44.000004	27
CGAATGC	20	6.952051E-4	44.000004	38
TGCCACC	20	6.952051E-4	44.000004	42
CTTACGA	20	6.952051E-4	44.000004	34
ATGCCAC	20	6.952051E-4	44.000004	41
TACGAAT	20	6.952051E-4	44.000004	36
GCCACCA	20	6.952051E-4	44.000004	43
CGGCCTT	20	6.952051E-4	44.000004	30
CCTTACG	20	6.952051E-4	44.000004	33
TAATACG	20	6.952051E-4	44.000004	25
>>END_MODULE
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
Read 552069 spots for SRR25943848.sra
Written 552069 spots for SRR25943848.sra
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
Read 552064 spots for SRR25943848.sra
Written 552064 spots for SRR25943848.sra
SRR ids: ['SRR25943848.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hfo088kp
SRR25943848.sra spots: 4416517
blocks: [[1, 552064], [552065, 1104128], [1104129, 1656192], [1656193, 2208256], [2208257, 2760320], [2760321, 3312384], [3312385, 3864448], [3864449, 4416517]]
SRR25943848 file size 763156
SRR25943848 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943848 SRR25943848_1.fastq
Input file:	SRR25943848_1.fastq
trimmed:	SRR25943848-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:36:56 2026 >> started

Tue May  5 05:36:58 2026 >> done (1.687s)
4416517 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
4416517 (100.00%) reads available; of these:
     68 ( 0.00%) trimmed reads available after processing
4416449 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      5	  0.00%
 44	      2	  0.00%
 45	      1	  0.00%
 46	     14	  0.00%
 47	      6	  0.00%
 48	     18	  0.00%
 49	     22	  0.00%
 50	4416449	100.00%
4416517 reads passed initial QC


criterion=sequence-density
sequence-density=14.46
sequence-density-rank=1
fanout-score=1.36
fanout-score-rank=8
prefix-density=0.04
prefix-fanout=1.4
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGT


criterion=fanout-score
sequence-density=0.71
sequence-density-rank=23
fanout-score=28.02
fanout-score-rank=1
prefix-density=19.57
prefix-fanout=1.0
sequence=CAGCGATCTGTAGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:37:05
                             Started mapping on |	May 05 05:37:05
                                    Finished on |	May 05 05:37:13
       Mapping speed, Million of reads per hour |	1987.43

                          Number of input reads |	4416517
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2827403
                        Uniquely mapped reads % |	64.02%
                          Average mapped length |	49.59
                       Number of splices: Total |	1882
            Number of splices: Annotated (sjdb) |	592
                       Number of splices: GT/AG |	1657
                       Number of splices: GC/AG |	29
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	196
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.10
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	546751
             % of reads mapped to multiple loci |	12.38%
        Number of reads mapped to too many loci |	891339
             % of reads mapped to too many loci |	20.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.42%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1042363	1042363	1042363
N_multimapping	546751	546751	546751
N_noFeature	8364	2721001	8378
N_ambiguous	129159	36	23893
UnstrandedReadsAssigned:2689880 PositiveStrandReadsAssigned:106366 NegativeStrandReadsAssigned:2795132
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943848 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943848-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,416,517 reads, 4,081,547 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,153 rounds

 109283 SRR25943848.ke.tsv
  52636 SRR25943848.se.tsv
 161919 total
==> SRR25943848.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943848.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943848 completed mapping pipeline successfully
