Starting /dee2/code/volunteer_pipeline.sh SRR25943811
    current disk space = 1264130196
    free memory = 193075312 
SRR25943811 SRAfilesize
8b4bfd8e1b00d979c25728955254e752  SRR25943811.sra
SRR25943811.sra file validated
SRR25943811 is single end
SRR25943811 is conventional basespace
SRR25943811 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943811_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51525	32.0	32.0	32.0	32.0	32.0
2	31.54175	32.0	32.0	32.0	32.0	32.0
3	31.576	32.0	32.0	32.0	32.0	32.0
4	31.70275	32.0	32.0	32.0	32.0	32.0
5	31.6195	32.0	32.0	32.0	32.0	32.0
6	35.2395	36.0	36.0	36.0	36.0	36.0
7	35.185	36.0	36.0	36.0	36.0	36.0
8	34.93225	36.0	36.0	36.0	32.0	36.0
9	34.951	36.0	36.0	36.0	36.0	36.0
10	35.0705	36.0	36.0	36.0	32.0	36.0
11	35.0745	36.0	36.0	36.0	36.0	36.0
12	35.12175	36.0	36.0	36.0	36.0	36.0
13	35.154	36.0	36.0	36.0	36.0	36.0
14	35.04525	36.0	36.0	36.0	36.0	36.0
15	35.094	36.0	36.0	36.0	36.0	36.0
16	35.00825	36.0	36.0	36.0	36.0	36.0
17	34.9315	36.0	36.0	36.0	32.0	36.0
18	34.90625	36.0	36.0	36.0	32.0	36.0
19	35.099	36.0	36.0	36.0	36.0	36.0
20	35.11975	36.0	36.0	36.0	36.0	36.0
21	34.94175	36.0	36.0	36.0	36.0	36.0
22	35.0275	36.0	36.0	36.0	36.0	36.0
23	35.12625	36.0	36.0	36.0	36.0	36.0
24	35.02975	36.0	36.0	36.0	36.0	36.0
25	35.19225	36.0	36.0	36.0	36.0	36.0
26	34.7775	36.0	36.0	36.0	32.0	36.0
27	34.84525	36.0	36.0	36.0	32.0	36.0
28	34.76025	36.0	36.0	36.0	32.0	36.0
29	34.991	36.0	36.0	36.0	36.0	36.0
30	34.78375	36.0	36.0	36.0	32.0	36.0
31	35.01125	36.0	36.0	36.0	36.0	36.0
32	34.78575	36.0	36.0	36.0	32.0	36.0
33	34.661	36.0	36.0	36.0	32.0	36.0
34	34.9455	36.0	36.0	36.0	32.0	36.0
35	34.90325	36.0	36.0	36.0	36.0	36.0
36	35.02625	36.0	36.0	36.0	36.0	36.0
37	34.74075	36.0	36.0	36.0	32.0	36.0
38	34.89675	36.0	36.0	36.0	32.0	36.0
39	34.904	36.0	36.0	36.0	32.0	36.0
40	34.55475	36.0	36.0	36.0	32.0	36.0
41	34.577	36.0	36.0	36.0	32.0	36.0
42	34.85925	36.0	36.0	36.0	32.0	36.0
43	34.5485	36.0	36.0	36.0	32.0	36.0
44	34.72275	36.0	36.0	36.0	32.0	36.0
45	34.48525	36.0	36.0	36.0	32.0	36.0
46	34.40075	36.0	36.0	36.0	32.0	36.0
47	34.5255	36.0	36.0	36.0	32.0	36.0
48	34.30875	36.0	36.0	36.0	32.0	36.0
49	34.5945	36.0	36.0	36.0	32.0	36.0
50	34.09175	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0714833563429984
11101	2	0.20190980211688725
11101	3	-0.04149409418622341
11101	4	0.10001533977603927
11101	5	-0.1719205399601158
11101	6	-0.08881730326736914
11101	7	-0.09529835864396574
11101	8	-0.34050467863168876
11101	9	-0.0597867771130538
11101	10	-0.367080840619721
11101	11	-0.08739837398373851
11101	12	-0.26637521092191463
11101	13	-0.05476300046019844
11101	14	0.19707777266452098
11101	15	-0.179321981899065
11101	16	0.09940174873447205
11101	17	-0.18936953520478994
11101	18	-0.22085442552538836
11101	19	-0.2548703788924698
11101	20	-0.18235158766682247
11101	21	8.436876821633632E-4
11101	22	-0.3485197116122123
11101	23	-0.14534437797208
11101	24	-0.37287160607455405
11101	25	0.07336247890781067
11101	26	-0.4055069795980941
11101	27	-0.1561205706396649
11101	28	-0.5355115815309119
11101	29	-0.16912103083294738
11101	30	0.2822518791225619
11101	31	0.1431201104463824
11101	32	-0.04410185611290274
11101	33	-0.10872066267832281
11101	34	0.005330572173647852
11101	35	-0.11635220125786105
11101	36	0.15769289768369532
11101	37	-0.13525847522625867
11101	38	-0.32965178708390397
11101	39	-0.3800813008130106
11101	40	-0.03378585672648882
11101	41	-0.47534131001687285
11101	42	-0.22169811320754462
11101	43	0.3722580150329833
11101	44	-0.1686224881116729
11101	45	-0.10277649946310419
11101	46	0.0319834330418729
11101	47	0.048243595643505444
11101	48	-0.2924911796287759
11101	49	-0.17015646571559984
11101	50	-0.269213069489183
11102	1	-0.0714833563429984
11102	2	-0.2019098021168908
11102	3	0.04149409418622696
11102	4	-0.10001533977603927
11102	5	0.17192053996011936
11102	6	0.08881730326737625
11102	7	0.09529835864395864
11102	8	0.34050467863169587
11102	9	0.0597867771130538
11102	10	0.3670808406197281
11102	11	0.08739837398374561
11102	12	0.26637521092192173
11102	13	0.054763000460191336
11102	14	-0.19707777266452098
11102	15	0.179321981899065
11102	16	-0.09940174873446495
11102	17	0.18936953520478284
11102	18	0.22085442552538126
11102	19	0.2548703788924698
11102	20	0.18235158766682247
11102	21	-8.436876821562578E-4
11102	22	0.3485197116122052
11102	23	0.1453443779720871
11102	24	0.37287160607454695
11102	25	-0.07336247890780356
11102	26	0.4055069795981012
11102	27	0.156120570639672
11102	28	0.5355115815309119
11102	29	0.16912103083294738
11102	30	-0.282251879122569
11102	31	-0.1431201104463895
11102	32	0.04410185611289563
11102	33	0.10872066267832992
11102	34	-0.005330572173647852
11102	35	0.11635220125786105
11102	36	-0.15769289768369532
11102	37	0.13525847522625867
11102	38	0.3296517870839111
11102	39	0.3800813008130106
11102	40	0.03378585672649592
11102	41	0.47534131001687285
11102	42	0.22169811320755173
11102	43	-0.3722580150329762
11102	44	0.1686224881116729
11102	45	0.10277649946311129
11102	46	-0.031983433041880005
11102	47	-0.048243595643505444
11102	48	0.2924911796287759
11102	49	0.17015646571559984
11102	50	0.269213069489183
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	1.0
24	3.0
25	7.0
26	8.0
27	26.0
28	49.0
29	66.0
30	93.0
31	117.0
32	161.0
33	318.0
34	612.0
35	2538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.45	19.55	23.599999999999998	25.4
2	24.725	23.375	20.825	31.075000000000003
3	23.125	23.875	28.7	24.3
4	22.375	21.475	31.424999999999997	24.725
5	18.05	21.6	29.775000000000002	30.575000000000003
6	29.02176632474356	27.145359019264447	23.567675756817614	20.26519889917438
7	29.25	18.375	31.974999999999998	20.4
8	26.55	17.95	21.375	34.125
9	26.3	18.7	23.325000000000003	31.674999999999997
10	23.075000000000003	17.1	30.3	29.525000000000002
11	23.150000000000002	21.4	20.974999999999998	34.475
12	30.049999999999997	17.575	21.525	30.85
13	26.724999999999998	31.874999999999996	21.175	20.225
14	27.325	22.15	22.425	28.1
15	18.85	29.45	23.325000000000003	28.375
16	18.6	25.874999999999996	32.175	23.35
17	22.275	17.2	23.7	36.825
18	23.849999999999998	17.9	36.525	21.725
19	20.1	29.15	29.225	21.525
20	25.85	15.975	21.675	36.5
21	21.099999999999998	21.775	29.45	27.675
22	19.45	18.2	32.75	29.599999999999998
23	28.249999999999996	12.625	26.900000000000002	32.225
24	31.924999999999997	18.525	19.400000000000002	30.15
25	1.5	5.525	81.75	11.225
26	18.65	26.400000000000002	19.525000000000002	35.425000000000004
27	28.175	26.900000000000002	16.275000000000002	28.65
28	18.3	25.25	28.65	27.800000000000004
29	28.375	23.575	18.25	29.799999999999997
30	24.474999999999998	22.925	28.15	24.45
31	26.5	21.75	18.95	32.800000000000004
32	26.200000000000003	20.025000000000002	22.475	31.3
33	29.275000000000002	25.2	21.7	23.825
34	24.3	19.0	32.75	23.95
35	23.375	18.675	24.85	33.1
36	21.875	14.2	31.225	32.7
37	20.474999999999998	30.049999999999997	28.075	21.4
38	25.1	15.225	25.25	34.425
39	25.0	26.8	23.075000000000003	25.124999999999996
40	21.075	27.750000000000004	24.275	26.900000000000002
41	23.225	23.175	24.075	29.525000000000002
42	22.075	22.875	24.925	30.125
43	21.8	24.45	31.474999999999998	22.275
44	22.35	18.425	25.624999999999996	33.6
45	16.075	22.900000000000002	20.9	40.125
46	20.9	30.675	27.975	20.45
47	28.999999999999996	16.45	17.925	36.625
48	23.7	32.225	21.925	22.15
49	25.174999999999997	18.4	34.55	21.875
50	18.25	15.525	19.325	46.9
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.5
32	1.0
33	1.0
34	1.0
35	4.0
36	7.0
37	18.0
38	29.0
39	41.0
40	53.0
41	195.5
42	338.0
43	275.5
44	213.0
45	280.5
46	348.0
47	268.0
48	188.0
49	366.5
50	545.0
51	549.0
52	553.0
53	430.0
54	307.0
55	474.0
56	641.0
57	475.5
58	310.0
59	261.5
60	213.0
61	149.5
62	86.0
63	83.5
64	81.0
65	61.0
66	41.0
67	30.0
68	19.0
69	17.0
70	15.0
71	12.0
72	9.0
73	5.0
74	1.0
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.4
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.66042780748663	27.175
2	11.497326203208557	8.6
3	4.3449197860962565	4.875
4	2.406417112299465	3.5999999999999996
5	2.339572192513369	4.375
6	0.9358288770053476	2.1
7	1.0026737967914439	2.625
8	0.6016042780748663	1.7999999999999998
9	0.2005347593582888	0.675
>10	3.4759358288770055	24.5
>50	0.4010695187165776	10.65
>100	0.1336898395721925	9.025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	201	5.025	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	160	4.0	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	94	2.35	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	85	2.125	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	69	1.725	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	68	1.7000000000000002	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	59	1.4749999999999999	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	51	1.275	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	43	1.075	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	40	1.0	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	40	1.0	No Hit
AGCTGAGCTCTCGACTCACAGATATTACTGGTGGATAGAGCCTCAGGGCC	38	0.95	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	32	0.8	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	29	0.7250000000000001	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	29	0.7250000000000001	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	28	0.7000000000000001	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	26	0.65	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	25	0.625	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	23	0.575	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	22	0.5499999999999999	No Hit
AGAGGCAGCACTTGCTGGAGAAGGTCAGGCGTCCTTGCGTCAGACTGGCT	22	0.5499999999999999	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	22	0.5499999999999999	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	21	0.525	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	21	0.525	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	21	0.525	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	20	0.5	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	20	0.5	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	20	0.5	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	19	0.475	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	18	0.44999999999999996	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	17	0.42500000000000004	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	17	0.42500000000000004	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	17	0.42500000000000004	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTAT	17	0.42500000000000004	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	16	0.4	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	16	0.4	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	16	0.4	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	16	0.4	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	15	0.375	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	15	0.375	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	15	0.375	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	15	0.375	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	14	0.35000000000000003	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	13	0.325	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	13	0.325	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	13	0.325	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	13	0.325	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	12	0.3	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	12	0.3	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	12	0.3	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	12	0.3	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	12	0.3	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	11	0.27499999999999997	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	11	0.27499999999999997	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	11	0.27499999999999997	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	10	0.25	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	10	0.25	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	10	0.25	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	10	0.25	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	10	0.25	No Hit
ACTTCTGGCACTAGGACCATAATGTCATGTCCTCGTTCACTGAGATGTTC	9	0.22499999999999998	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	9	0.22499999999999998	No Hit
TGGAGAAACTCGTGTGAGGATTAATCAGGTAGAAAGAGACTATGCAGGGT	9	0.22499999999999998	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	8	0.2	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	8	0.2	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	8	0.2	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	8	0.2	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	8	0.2	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	8	0.2	No Hit
TCTTTACCACTGATCCACAATCAACCCGTTTTCTACCAACAGTATAGGAG	8	0.2	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	8	0.2	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	8	0.2	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	7	0.17500000000000002	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	7	0.17500000000000002	No Hit
TTCTACACTGCCTACTGCTTACATTTTCCAAACCCATCAGCATCACTCCT	7	0.17500000000000002	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	7	0.17500000000000002	No Hit
CTAGCACATTCTCTGATGGCACCTTCACTCCATCAAAGTACACCTCTGAC	7	0.17500000000000002	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	7	0.17500000000000002	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	7	0.17500000000000002	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	7	0.17500000000000002	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	7	0.17500000000000002	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	7	0.17500000000000002	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	7	0.17500000000000002	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	7	0.17500000000000002	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	7	0.17500000000000002	No Hit
CCCAAGTAGGTGATGCCAGAATCCTCGTAGAAGCTAGCACTGGTGTTGAC	7	0.17500000000000002	No Hit
AAACAGGCATTGGTGATCTCTGCCACTGACAGCTGCTCATGGTAGGCCTT	6	0.15	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	6	0.15	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	6	0.15	No Hit
AGGTGGGTCCCACCGATTTGCCAGCGCTCTGGGCGCTCACCCCAGTGAAT	6	0.15	No Hit
CTCTCCGCACCTTCTGCCAGCTTGTTCACCATACTAGCATTTCCAGAAGC	6	0.15	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	6	0.15	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	6	0.15	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	6	0.15	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	6	0.15	No Hit
CCAGCTGCCCTTGAGTGATGTACTTTTTCCACCAGAGGTAGGGACGCATG	6	0.15	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	6	0.15	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	6	0.15	No Hit
GCCATGAGGTCCACCACCCTGTTGCTGTAGCCGTATTCATTGTCATACCA	6	0.15	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	5	0.125	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	5	0.125	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	5	0.125	No Hit
TTGGCAGGCTTGGTTTCCTCGATCTCAGAAGACAGCCATCGGTAGGTACG	5	0.125	No Hit
GCAGACGCAACTCTCGGTAGGTCCTTTTGGCGTGAATGATGGACTGAAAC	5	0.125	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	5	0.125	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	5	0.125	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	5	0.125	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	5	0.125	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	5	0.125	No Hit
AGCACTTTACAATGAGGGCTACAATGAATGTCACAAAAGCAAGCAGGAAT	5	0.125	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
GTGGCTCATTCCAGAAGCTGAACTCCAGAAGCAACTGGTTTCTCCTGGAC	5	0.125	No Hit
TGGAAGGACACTGGTCAGCAATCCTCAACACAGCACGCCACTTCCCAAAG	5	0.125	No Hit
GGCAAATACACAGAGGAAGCCCTCCCCTGTGCGCATGTACTGGTCCCGCA	5	0.125	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	5	0.125	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	5	0.125	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
CTCTCAGGATCCAGGCTTCCTTTCCCCACTTTCCTCATGCATGTCACTAC	5	0.125	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	5	0.125	No Hit
CCTGAGAGACAGAGCAGGCCTGTTCTTCATGTACTTGTTACCCAGCCATA	5	0.125	No Hit
GAACGCCGCAGGTGAGAGGCCATTTCATAACATTTCTTGTTGATCAAACC	5	0.125	No Hit
CGCCTTGCAACGCGAGTCTGTGTTTTTGCAGGAACATTTACACGTCTGCG	5	0.125	No Hit
GTACTTTGACAGCTTCCACCTCATTGGCTGTTGAGTCCATCTTAGCAATG	5	0.125	No Hit
CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTCGTA	5	0.125	No Hit
AACAGCTCCTTGAGAACACGGTCCTGACTCTGCACGGTGTGCACGATGAT	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTA	5	0.125	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	5	0.125	No Hit
GCTCTTACATCACTGCTCTGTGTCTTCTCCAATATCCAGTCCATGTACTC	5	0.125	No Hit
CTCACCTGTGCTCAGATCCACACCTACGAGCTGGCCGGATTCCGAATGTT	5	0.125	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	5	0.125	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTGCCCACTGGCGATGCATGC	5	0.125	No Hit
CTGTGTTCTCCCTACAACTGTGTGTGTGATCCTCCTCAATGCTTCCCTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTCCA	20	6.952051E-4	44.000004	40
CAGTTCT	20	6.952051E-4	44.000004	12
CCAGTTC	20	6.952051E-4	44.000004	11
TTTCCAC	20	6.952051E-4	44.000004	41
GTTCTAC	20	6.952051E-4	44.000004	14
ACTCCTT	20	6.952051E-4	44.000004	28
ACACTCC	20	6.952051E-4	44.000004	26
TCTACTC	20	6.952051E-4	44.000004	16
TCCAGTT	20	6.952051E-4	44.000004	10
TACACTC	20	6.952051E-4	44.000004	25
CACTCCT	20	6.952051E-4	44.000004	27
GTCCAGT	20	6.952051E-4	44.000004	9
GATTTGG	20	6.952051E-4	44.000004	1
ATTTGGT	20	6.952051E-4	44.000004	2
TTCTACT	20	6.952051E-4	44.000004	15
TTTGGTG	20	6.952051E-4	44.000004	3
TTGGTGT	20	6.952051E-4	44.000004	4
TGGTGTC	20	6.952051E-4	44.000004	5
AGTTCTA	20	6.952051E-4	44.000004	13
CTACTCT	20	6.952051E-4	44.000004	17
>>END_MODULE
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
Read 329611 spots for SRR25943811.sra
Written 329611 spots for SRR25943811.sra
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
Read 329605 spots for SRR25943811.sra
Written 329605 spots for SRR25943811.sra
SRR ids: ['SRR25943811.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__jg2w9s7
SRR25943811.sra spots: 2636846
blocks: [[1, 329605], [329606, 659210], [659211, 988815], [988816, 1318420], [1318421, 1648025], [1648026, 1977630], [1977631, 2307235], [2307236, 2636846]]
SRR25943811 file size 455204
SRR25943811 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943811 SRR25943811_1.fastq
Input file:	SRR25943811_1.fastq
trimmed:	SRR25943811-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:43:49 2026 >> started

Tue May  5 05:43:50 2026 >> done (0.661s)
2636846 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2636846 (100.00%) reads available; of these:
     54 ( 0.00%) trimmed reads available after processing
2636792 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      1	  0.00%
 44	      3	  0.00%
 45	      0	  0.00%
 46	     14	  0.00%
 47	      6	  0.00%
 48	     13	  0.00%
 49	     17	  0.00%
 50	2636792	100.00%
2636846 reads passed initial QC


criterion=sequence-density
sequence-density=5.76
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATCCCTGTG


criterion=fanout-score
sequence-density=3.55
sequence-density-rank=3
fanout-score=15.86
fanout-score-rank=1
prefix-density=3.74
prefix-fanout=15.0
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:43:56
                             Started mapping on |	May 05 05:43:56
                                    Finished on |	May 05 05:44:01
       Mapping speed, Million of reads per hour |	1898.53

                          Number of input reads |	2636846
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2144817
                        Uniquely mapped reads % |	81.34%
                          Average mapped length |	49.55
                       Number of splices: Total |	1412
            Number of splices: Annotated (sjdb) |	716
                       Number of splices: GT/AG |	1244
                       Number of splices: GC/AG |	30
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	137
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.09%
                        Deletion average length |	1.18
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	220226
             % of reads mapped to multiple loci |	8.35%
        Number of reads mapped to too many loci |	122198
             % of reads mapped to too many loci |	4.63%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.67%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	271803	271803	271803
N_multimapping	220226	220226	220226
N_noFeature	1753	2095738	1782
N_ambiguous	70061	15	21488
UnstrandedReadsAssigned:2073003 PositiveStrandReadsAssigned:49064 NegativeStrandReadsAssigned:2121547
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943811 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943811-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,636,846 reads, 2,355,505 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,154 rounds

 109283 SRR25943811.ke.tsv
  52636 SRR25943811.se.tsv
 161919 total
==> SRR25943811.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943811.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943811 completed mapping pipeline successfully
