Starting /dee2/code/volunteer_pipeline.sh SRR25943843
    current disk space = 1265776200
    free memory = 192285192 
SRR25943843 SRAfilesize
02c8450f50c697d9d01684fb6973ecec  SRR25943843.sra
SRR25943843.sra file validated
SRR25943843 is single end
SRR25943843 is conventional basespace
SRR25943843 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39875	32.0	32.0	32.0	32.0	32.0
2	31.4475	32.0	32.0	32.0	32.0	32.0
3	31.40775	32.0	32.0	32.0	32.0	32.0
4	31.642	32.0	32.0	32.0	32.0	32.0
5	31.6265	32.0	32.0	32.0	32.0	32.0
6	35.13	36.0	36.0	36.0	36.0	36.0
7	35.18	36.0	36.0	36.0	36.0	36.0
8	34.959	36.0	36.0	36.0	32.0	36.0
9	34.95025	36.0	36.0	36.0	32.0	36.0
10	35.04075	36.0	36.0	36.0	32.0	36.0
11	34.86625	36.0	36.0	36.0	32.0	36.0
12	35.037	36.0	36.0	36.0	36.0	36.0
13	35.20075	36.0	36.0	36.0	36.0	36.0
14	34.947	36.0	36.0	36.0	36.0	36.0
15	35.0265	36.0	36.0	36.0	36.0	36.0
16	34.9765	36.0	36.0	36.0	32.0	36.0
17	34.9885	36.0	36.0	36.0	36.0	36.0
18	34.7985	36.0	36.0	36.0	32.0	36.0
19	35.072	36.0	36.0	36.0	36.0	36.0
20	34.9145	36.0	36.0	36.0	36.0	36.0
21	34.98775	36.0	36.0	36.0	36.0	36.0
22	34.9305	36.0	36.0	36.0	36.0	36.0
23	34.993	36.0	36.0	36.0	36.0	36.0
24	34.996	36.0	36.0	36.0	36.0	36.0
25	34.88425	36.0	36.0	36.0	36.0	36.0
26	34.88025	36.0	36.0	36.0	36.0	36.0
27	34.874	36.0	36.0	36.0	36.0	36.0
28	34.51775	36.0	36.0	36.0	32.0	36.0
29	34.96325	36.0	36.0	36.0	36.0	36.0
30	34.93625	36.0	36.0	36.0	32.0	36.0
31	35.01675	36.0	36.0	36.0	36.0	36.0
32	34.8435	36.0	36.0	36.0	32.0	36.0
33	34.978	36.0	36.0	36.0	32.0	36.0
34	35.00425	36.0	36.0	36.0	36.0	36.0
35	34.96975	36.0	36.0	36.0	36.0	36.0
36	34.89475	36.0	36.0	36.0	32.0	36.0
37	34.69	36.0	36.0	36.0	32.0	36.0
38	34.969	36.0	36.0	36.0	32.0	36.0
39	34.91075	36.0	36.0	36.0	32.0	36.0
40	34.55275	36.0	36.0	36.0	32.0	36.0
41	34.7895	36.0	36.0	36.0	32.0	36.0
42	34.7215	36.0	36.0	36.0	32.0	36.0
43	34.73775	36.0	36.0	36.0	32.0	36.0
44	34.54475	36.0	36.0	36.0	32.0	36.0
45	34.47175	36.0	36.0	36.0	32.0	36.0
46	34.5775	36.0	36.0	36.0	32.0	36.0
47	34.165	36.0	36.0	36.0	32.0	36.0
48	34.28375	36.0	36.0	36.0	32.0	36.0
49	34.49175	36.0	36.0	36.0	32.0	36.0
50	33.98125	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	2.0
25	8.0
26	13.0
27	27.0
28	52.0
29	54.0
30	93.0
31	157.0
32	186.0
33	300.0
34	580.0
35	2527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.424999999999997	10.475	30.825000000000003	38.275
2	15.6	14.299999999999999	36.475	33.625
3	45.125	13.975000000000001	11.450000000000001	29.45
4	22.675	11.125	13.575000000000001	52.625
5	28.025	18.35	20.849999999999998	32.775
6	37.275000000000006	18.4	15.85	28.475
7	38.125	10.75	20.875	30.25
8	14.674999999999999	27.725	13.025	44.574999999999996
9	31.275	30.25	19.275000000000002	19.2
10	16.875	10.375	38.175	34.575
11	31.474999999999998	41.949999999999996	11.0	15.575
12	25.95	13.625000000000002	46.2	14.224999999999998
13	43.6	16.075	10.875	29.45
14	25.974999999999998	13.8	38.65	21.575
15	28.575	40.125	13.8	17.5
16	10.299999999999999	39.45	34.849999999999994	15.4
17	28.525	12.975	35.65	22.85
18	30.475	13.700000000000001	42.0	13.825000000000001
19	32.05	31.45	21.95	14.549999999999999
20	21.55	10.125	31.974999999999998	36.35
21	9.9	11.600000000000001	60.9	17.599999999999998
22	15.625	11.05	54.65	18.675
23	37.35	10.299999999999999	16.025	36.325
24	15.775	15.0	33.650000000000006	35.575
25	1.6	2.8000000000000003	72.82499999999999	22.775000000000002
26	25.825	19.375	29.4	25.4
27	36.65	16.6	14.899999999999999	31.85
28	11.75	16.7	37.3	34.25
29	20.849999999999998	31.424999999999997	9.375	38.35
30	15.950000000000001	29.075	37.175000000000004	17.8
31	41.375	14.875	11.774999999999999	31.974999999999998
32	19.525000000000002	12.675	31.275	36.525
33	19.975	32.324999999999996	27.925	19.775000000000002
34	12.825000000000001	33.175	38.9	15.1
35	15.575	11.025	39.85	33.550000000000004
36	36.75	8.0	23.05	32.2
37	35.65	36.725	14.35	13.275
38	38.975	7.9750000000000005	29.299999999999997	23.75
39	36.7	18.5	13.450000000000001	31.35
40	14.025000000000002	17.65	31.324999999999996	37.0
41	13.65	33.525	15.950000000000001	36.875
42	34.125	11.875	20.875	33.125
43	16.2	15.299999999999999	31.35	37.15
44	17.625	11.725	37.075	33.575
45	8.75	34.425	29.675	27.150000000000002
46	13.425	19.0	38.175	29.4
47	36.1	8.799999999999999	13.450000000000001	41.65
48	11.555777888944473	16.18309154577289	55.25262631315658	17.008504252126063
49	20.849999999999998	14.149999999999999	31.874999999999996	33.125
50	9.9	18.224999999999998	12.85	59.025000000000006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	3.5
30	7.0
31	5.5
32	4.0
33	2.5
34	1.0
35	1.0
36	1.0
37	11.0
38	21.0
39	39.5
40	58.0
41	115.5
42	173.0
43	236.0
44	299.0
45	226.0
46	153.0
47	130.5
48	108.0
49	278.5
50	449.0
51	694.0
52	939.0
53	571.5
54	204.0
55	242.5
56	281.0
57	571.5
58	862.0
59	574.5
60	287.0
61	190.5
62	94.0
63	57.5
64	21.0
65	18.5
66	16.0
67	14.0
68	12.0
69	8.5
70	5.0
71	4.5
72	4.0
73	2.0
74	0.0
75	0.5
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.05
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96616541353383	19.675
2	11.74812030075188	6.25
3	4.6992481203007515	3.75
4	1.9736842105263157	2.1
5	1.0338345864661653	1.375
6	1.0338345864661653	1.6500000000000001
7	0.7518796992481203	1.4000000000000001
8	0.6578947368421052	1.4000000000000001
9	0.4699248120300752	1.125
>10	3.101503759398496	16.075
>50	0.09398496240601503	2.025
>100	0.28195488721804507	12.325
>500	0.18796992481203006	30.85
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	653	16.325	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	581	14.524999999999999	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	190	4.75	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	180	4.5	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	123	3.075	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	81	2.025	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	50	1.25	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	44	1.0999999999999999	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	31	0.775	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	31	0.775	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	30	0.75	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	27	0.675	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	27	0.675	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	24	0.6	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	23	0.575	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	22	0.5499999999999999	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	21	0.525	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	20	0.5	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	19	0.475	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	18	0.44999999999999996	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	18	0.44999999999999996	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	17	0.42500000000000004	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	16	0.4	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	15	0.375	No Hit
CTGACCAGGATGGAGGTGACATTGTAGCTATTGTTCTCTTGTAGCTCATA	15	0.375	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	15	0.375	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	15	0.375	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	14	0.35000000000000003	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	13	0.325	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	13	0.325	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCAT	13	0.325	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	13	0.325	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	13	0.325	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	12	0.3	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	12	0.3	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	12	0.3	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	10	0.25	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	10	0.25	No Hit
CCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTCC	10	0.25	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	9	0.22499999999999998	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	9	0.22499999999999998	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	9	0.22499999999999998	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	9	0.22499999999999998	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	9	0.22499999999999998	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	8	0.2	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	8	0.2	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	8	0.2	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	8	0.2	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	8	0.2	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCATCTC	8	0.2	No Hit
CTGGGTCGGATCTTGTGTTCATCCTGTTGCTCTCCCCTGGCGTCTGCACA	7	0.17500000000000002	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTCCAGCCAGCTTCCTTCC	7	0.17500000000000002	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCATGAC	7	0.17500000000000002	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	7	0.17500000000000002	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	7	0.17500000000000002	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	7	0.17500000000000002	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	7	0.17500000000000002	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	7	0.17500000000000002	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	6	0.15	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCATGGTGTCCTCATGTCCC	6	0.15	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	6	0.15	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCAGCTTCCTTCC	6	0.15	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	6	0.15	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	6	0.15	No Hit
ATTCAACAATGCCAGAGGAGGACTCCCAAGTGGTACATAGGTGTACTGGA	6	0.15	No Hit
AAGGTATGCCCGGCAGTATGGACATGTCCATTTATTATTGTTCTTTATAC	6	0.15	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	6	0.15	No Hit
GCTCTTACATCACTGCTCTGTGTCTTCTCCAATATCCAGTCCATGTACTC	6	0.15	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	6	0.15	No Hit
CTGGCTGTCCAATCCACCGACTATTAACACATGTCACAGTGTCTGAGCCA	5	0.125	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	5	0.125	No Hit
AGAAAGGTCAGAGAAGGGATGATATTCACTAGGTCAGCAGCAAATCCTTC	5	0.125	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	5	0.125	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	5	0.125	No Hit
GTACTTTGACAGCTTCCACCTCATTGGCTGTTGAGTCCATCTTAGCAATG	5	0.125	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	5	0.125	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	5	0.125	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	5	0.125	No Hit
CTTGGAGAGATTGAAATAGGTCTCTTTGATATCAAAGTCCTTGTGGATGA	5	0.125	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCCAG	45	2.728484E-10	44.000004	36
AGCCAGC	45	2.728484E-10	44.000004	37
TGGTACA	20	6.952051E-4	44.000004	7
TCCAGCC	45	2.728484E-10	44.000004	34
GCCAGCT	45	2.728484E-10	44.000004	38
GGTACAC	20	6.952051E-4	44.000004	8
TCCCCCG	45	2.728484E-10	44.000004	1
ATCCTAG	30	1.9515464E-6	44.0	6
AAGTATT	50	1.4551915E-11	44.0	15
TGTTGGC	70	0.0	44.0	14
GAGGTAT	30	1.9515464E-6	44.0	1
CAATGGT	70	0.0	44.0	32
ATCTTTC	50	1.4551915E-11	44.0	29
GTGTCCT	70	0.0	44.0	37
CAGCAAT	70	0.0	44.0	29
TCCTAGG	30	1.9515464E-6	44.0	7
GGACTTC	30	1.9515464E-6	44.0	37
TGTCTGT	70	0.0	44.0	10
CATCTTT	50	1.4551915E-11	44.0	28
GAAGTAT	50	1.4551915E-11	44.0	14
>>END_MODULE
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
Read 434968 spots for SRR25943843.sra
Written 434968 spots for SRR25943843.sra
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
Read 434964 spots for SRR25943843.sra
Written 434964 spots for SRR25943843.sra
SRR ids: ['SRR25943843.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9ku3vsy4
SRR25943843.sra spots: 3479716
blocks: [[1, 434964], [434965, 869928], [869929, 1304892], [1304893, 1739856], [1739857, 2174820], [2174821, 2609784], [2609785, 3044748], [3044749, 3479716]]
SRR25943843 file size 601052
SRR25943843 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943843 SRR25943843_1.fastq
Input file:	SRR25943843_1.fastq
trimmed:	SRR25943843-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:36:49 2026 >> started

Tue May  5 05:36:50 2026 >> done (0.974s)
3479716 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3479716 (100.00%) reads available; of these:
     76 ( 0.00%) trimmed reads available after processing
3479640 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      2	  0.00%
 44	      1	  0.00%
 45	      1	  0.00%
 46	      9	  0.00%
 47	      8	  0.00%
 48	     23	  0.00%
 49	     32	  0.00%
 50	3479640	100.00%
3479716 reads passed initial QC


criterion=sequence-density
sequence-density=18.67
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=19
prefix-density=0.00
prefix-fanout=1.0
sequence=CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCCCCTG


criterion=fanout-score
sequence-density=0.32
sequence-density-rank=37
fanout-score=19.26
fanout-score-rank=1
prefix-density=6.19
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:36:56
                             Started mapping on |	May 05 05:36:56
                                    Finished on |	May 05 05:37:01
       Mapping speed, Million of reads per hour |	2505.40

                          Number of input reads |	3479716
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2757337
                        Uniquely mapped reads % |	79.24%
                          Average mapped length |	49.60
                       Number of splices: Total |	1327
            Number of splices: Annotated (sjdb) |	529
                       Number of splices: GT/AG |	1173
                       Number of splices: GC/AG |	19
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	135
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.06%
                        Deletion average length |	1.08
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	240133
             % of reads mapped to multiple loci |	6.90%
        Number of reads mapped to too many loci |	407137
             % of reads mapped to too many loci |	11.70%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.16%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	482246	482246	482246
N_multimapping	240133	240133	240133
N_noFeature	5508	2728052	5520
N_ambiguous	43952	17	15089
UnstrandedReadsAssigned:2707877 PositiveStrandReadsAssigned:29268 NegativeStrandReadsAssigned:2736728
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943843 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943843-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,479,716 reads, 3,275,922 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,094 rounds

 109283 SRR25943843.ke.tsv
  52636 SRR25943843.se.tsv
 161919 total
==> SRR25943843.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943843.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943843 completed mapping pipeline successfully
