Starting /dee2/code/volunteer_pipeline.sh SRR25943836
    current disk space = 1266460644
    free memory = 192098980 
SRR25943836 SRAfilesize
6dcafcb0e01e1ba3080109c6e9891d2a  SRR25943836.sra
SRR25943836.sra file validated
SRR25943836 is single end
SRR25943836 is conventional basespace
SRR25943836 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943836_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42	32.0	32.0	32.0	32.0	32.0
2	31.58125	32.0	32.0	32.0	32.0	32.0
3	31.56175	32.0	32.0	32.0	32.0	32.0
4	31.6225	32.0	32.0	32.0	32.0	32.0
5	31.64	32.0	32.0	32.0	32.0	32.0
6	35.01025	36.0	36.0	36.0	36.0	36.0
7	35.051	36.0	36.0	36.0	36.0	36.0
8	34.95675	36.0	36.0	36.0	36.0	36.0
9	35.06275	36.0	36.0	36.0	36.0	36.0
10	35.046	36.0	36.0	36.0	36.0	36.0
11	35.0275	36.0	36.0	36.0	36.0	36.0
12	35.21325	36.0	36.0	36.0	36.0	36.0
13	35.1895	36.0	36.0	36.0	36.0	36.0
14	35.07175	36.0	36.0	36.0	36.0	36.0
15	35.006	36.0	36.0	36.0	36.0	36.0
16	34.907	36.0	36.0	36.0	36.0	36.0
17	35.01	36.0	36.0	36.0	36.0	36.0
18	34.95025	36.0	36.0	36.0	36.0	36.0
19	35.05625	36.0	36.0	36.0	36.0	36.0
20	35.08	36.0	36.0	36.0	36.0	36.0
21	35.013	36.0	36.0	36.0	36.0	36.0
22	35.02225	36.0	36.0	36.0	36.0	36.0
23	35.00675	36.0	36.0	36.0	36.0	36.0
24	34.97525	36.0	36.0	36.0	36.0	36.0
25	35.1575	36.0	36.0	36.0	36.0	36.0
26	34.9085	36.0	36.0	36.0	32.0	36.0
27	34.9565	36.0	36.0	36.0	36.0	36.0
28	34.847	36.0	36.0	36.0	32.0	36.0
29	35.03175	36.0	36.0	36.0	36.0	36.0
30	34.9025	36.0	36.0	36.0	32.0	36.0
31	34.94325	36.0	36.0	36.0	36.0	36.0
32	34.83125	36.0	36.0	36.0	32.0	36.0
33	34.8855	36.0	36.0	36.0	32.0	36.0
34	34.90825	36.0	36.0	36.0	32.0	36.0
35	34.92075	36.0	36.0	36.0	32.0	36.0
36	34.8735	36.0	36.0	36.0	32.0	36.0
37	34.85	36.0	36.0	36.0	32.0	36.0
38	34.7395	36.0	36.0	36.0	32.0	36.0
39	34.87375	36.0	36.0	36.0	32.0	36.0
40	34.58525	36.0	36.0	36.0	32.0	36.0
41	34.6675	36.0	36.0	36.0	32.0	36.0
42	34.7365	36.0	36.0	36.0	32.0	36.0
43	34.4865	36.0	36.0	36.0	32.0	36.0
44	34.62675	36.0	36.0	36.0	32.0	36.0
45	34.43125	36.0	36.0	36.0	32.0	36.0
46	34.22725	36.0	36.0	36.0	32.0	36.0
47	34.153	36.0	36.0	36.0	32.0	36.0
48	34.15975	36.0	36.0	36.0	32.0	36.0
49	34.279	36.0	36.0	36.0	32.0	36.0
50	33.75775	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.06392045454545325
11101	2	0.03064123376623229
11101	3	-0.06919642857142705
11101	4	-0.12317370129870042
11101	5	-0.12276785714285765
11101	6	-0.1769480519480524
11101	7	-0.11221590909090651
11101	8	-0.18709415584415723
11101	9	0.15523538961038952
11101	10	-0.008319805194801688
11101	11	-0.060064935064936265
11101	12	0.41883116883116855
11101	13	0.13798701298701133
11101	14	0.16761363636364024
11101	15	-0.33847402597402976
11101	16	-0.144480519480517
11101	17	0.22199675324675638
11101	18	0.010957792207790362
11101	19	-0.3382711038961048
11101	20	0.08887987012986542
11101	21	-0.15706168831169265
11101	22	0.07995129870129603
11101	23	0.004464285714284699
11101	24	-0.2889610389610411
11101	25	-0.25527597402597024
11101	26	0.11870941558441217
11101	27	-0.6006493506493484
11101	28	-0.11038961038961048
11101	29	-0.029220779220779036
11101	30	-0.1428571428571459
11101	31	0.11891233766233711
11101	32	-0.12621753246753542
11101	33	-0.0714285714285765
11101	34	0.03875811688312325
11101	35	-0.1568587662337677
11101	36	0.038149350649348435
11101	37	0.08076298701298867
11101	38	0.2098214285714235
11101	39	-0.2025162337662323
11101	40	0.1623376623376629
11101	41	0.07244318181818699
11101	42	-0.05235389610389518
11101	43	-0.2526379870129887
11101	44	0.1339285714285765
11101	45	0.0050730519480524094
11101	46	0.2838879870129887
11101	47	-0.07609577922077904
11101	48	-0.02069805194805241
11101	49	0.3634334415584419
11101	50	-0.01684253246753542
11102	1	-0.0639204545454568
11102	2	-0.030641233766235842
11102	3	0.0691964285714306
11102	4	0.12317370129870397
11102	5	0.12276785714285765
11102	6	0.1769480519480524
11102	7	0.11221590909091361
11102	8	0.18709415584415012
11102	9	-0.15523538961038952
11102	10	0.008319805194808794
11102	11	0.060064935064936265
11102	12	-0.41883116883116855
11102	13	-0.13798701298701133
11102	14	-0.16761363636363313
11102	15	0.33847402597402265
11102	16	0.144480519480517
11102	17	-0.22199675324675638
11102	18	-0.010957792207790362
11102	19	0.3382711038960977
11102	20	-0.08887987012987253
11102	21	0.15706168831168554
11102	22	-0.07995129870130313
11102	23	-0.004464285714284699
11102	24	0.2889610389610411
11102	25	0.25527597402597735
11102	26	-0.11870941558441928
11102	27	0.6006493506493555
11102	28	0.11038961038961048
11102	29	0.029220779220779036
11102	30	0.1428571428571388
11102	31	-0.11891233766233711
11102	32	0.12621753246752832
11102	33	0.0714285714285694
11102	34	-0.038758116883116145
11102	35	0.1568587662337677
11102	36	-0.03814935064935554
11102	37	-0.08076298701298867
11102	38	-0.2098214285714306
11102	39	0.2025162337662323
11102	40	-0.1623376623376629
11102	41	-0.07244318181817988
11102	42	0.05235389610390229
11102	43	0.2526379870129887
11102	44	-0.1339285714285694
11102	45	-0.0050730519480524094
11102	46	-0.2838879870129887
11102	47	0.07609577922077904
11102	48	0.02069805194805241
11102	49	-0.3634334415584419
11102	50	0.016842532467528315
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	5.0
25	4.0
26	14.0
27	24.0
28	37.0
29	66.0
30	103.0
31	144.0
32	196.0
33	271.0
34	616.0
35	2518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.849999999999998	23.549999999999997	24.825	27.775
2	22.35	17.150000000000002	30.175	30.325000000000003
3	29.15	23.549999999999997	21.825	25.474999999999998
4	27.625	21.325	24.25	26.8
5	17.224999999999998	28.825	22.625	31.324999999999996
6	32.98324581145287	22.55563890972743	22.455613903475868	22.005501375343837
7	28.825	22.25	28.249999999999996	20.674999999999997
8	21.2	17.974999999999998	21.099999999999998	39.725
9	25.025	18.05	25.874999999999996	31.05
10	24.6	19.225	23.974999999999998	32.2
11	28.125	20.875	20.05	30.95
12	30.825000000000003	18.625	20.175	30.375000000000004
13	28.349999999999998	23.599999999999998	24.65	23.400000000000002
14	22.775000000000002	17.375	24.45	35.4
15	21.0	23.375	21.8	33.825
16	19.75	26.450000000000003	27.200000000000003	26.6
17	18.85	20.05	29.075	32.025
18	21.875	16.875	36.85	24.4
19	23.599999999999998	20.150000000000002	28.95	27.3
20	25.924999999999997	13.175	26.900000000000002	34.0
21	20.325	20.599999999999998	26.424999999999997	32.65
22	17.125	22.35	33.15	27.375
23	28.725	16.625	21.925	32.725
24	25.924999999999997	12.675	24.25	37.15
25	3.025	6.575	74.75	15.65
26	18.025	17.775	20.3	43.9
27	26.55	19.7	25.775	27.975
28	16.650000000000002	21.725	27.474999999999998	34.150000000000006
29	34.975	21.725	17.150000000000002	26.150000000000002
30	30.85	18.925	20.75	29.475
31	26.974999999999998	25.95	20.5	26.575
32	20.05	21.075	22.400000000000002	36.475
33	30.125	20.200000000000003	18.275	31.4
34	27.625	17.125	33.275	21.975
35	27.925	20.875	21.224999999999998	29.975
36	29.549999999999997	16.025	19.675	34.75
37	21.675	27.700000000000003	26.35	24.275
38	31.624999999999996	12.5	21.525	34.35
39	29.65	19.625	20.375	30.349999999999998
40	21.3	21.275	27.325	30.099999999999998
41	21.975	23.75	20.150000000000002	34.125
42	31.525	21.85	19.325	27.3
43	24.8	23.225	25.3	26.674999999999997
44	27.35	16.475	23.45	32.725
45	18.825	26.75	18.6	35.825
46	19.425	22.775000000000002	33.225	24.575
47	23.9	18.975	19.7	37.425000000000004
48	23.599999999999998	23.549999999999997	23.825	29.025000000000002
49	28.175	17.05	31.225	23.549999999999997
50	20.075000000000003	14.299999999999999	23.9	41.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	1.5
32	3.0
33	3.0
34	3.0
35	4.0
36	5.0
37	12.5
38	20.0
39	23.5
40	27.0
41	74.0
42	121.0
43	94.5
44	68.0
45	120.5
46	173.0
47	218.0
48	263.0
49	392.0
50	521.0
51	539.0
52	557.0
53	442.0
54	327.0
55	401.5
56	476.0
57	471.5
58	467.0
59	448.0
60	429.0
61	312.5
62	196.0
63	158.0
64	120.0
65	101.0
66	82.0
67	65.5
68	49.0
69	42.0
70	35.0
71	42.5
72	50.0
73	27.0
74	4.0
75	3.5
76	3.0
77	1.5
78	0.0
79	0.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09450830140486	28.225
2	11.047254150702427	8.649999999999999
3	4.980842911877394	5.8500000000000005
4	3.1928480204342273	5.0
5	1.40485312899106	2.75
6	1.40485312899106	3.3000000000000003
7	0.5747126436781609	1.575
8	0.7662835249042145	2.4
9	0.70242656449553	2.475
>10	3.5759897828863343	29.425
>50	0.19157088122605362	4.725
>100	0.06385696040868455	5.625
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	225	5.625	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	67	1.675	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	64	1.6	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	58	1.4500000000000002	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	50	1.25	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	49	1.225	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	48	1.2	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	46	1.15	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	45	1.125	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	45	1.125	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	42	1.05	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	41	1.0250000000000001	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	37	0.9249999999999999	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	34	0.8500000000000001	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	34	0.8500000000000001	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	32	0.8	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	31	0.775	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	27	0.675	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	26	0.65	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCAT	26	0.65	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	23	0.575	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	23	0.575	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	22	0.5499999999999999	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	21	0.525	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	19	0.475	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	19	0.475	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	18	0.44999999999999996	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	17	0.42500000000000004	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	17	0.42500000000000004	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	16	0.4	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	15	0.375	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	15	0.375	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	15	0.375	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	15	0.375	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	14	0.35000000000000003	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	14	0.35000000000000003	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	14	0.35000000000000003	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	14	0.35000000000000003	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	14	0.35000000000000003	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	13	0.325	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	13	0.325	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	13	0.325	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	13	0.325	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	12	0.3	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	12	0.3	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	12	0.3	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	12	0.3	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	12	0.3	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	11	0.27499999999999997	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	11	0.27499999999999997	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	11	0.27499999999999997	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	11	0.27499999999999997	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	11	0.27499999999999997	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	11	0.27499999999999997	No Hit
CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTA	11	0.27499999999999997	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	10	0.25	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	10	0.25	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	10	0.25	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	10	0.25	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	10	0.25	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	9	0.22499999999999998	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTT	9	0.22499999999999998	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	9	0.22499999999999998	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	9	0.22499999999999998	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	9	0.22499999999999998	No Hit
CGGCGCCAATTCCTTTCACCGAGTTCGCGAGGCAAGACGCGGAGACTCGC	9	0.22499999999999998	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	9	0.22499999999999998	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	9	0.22499999999999998	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	9	0.22499999999999998	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	9	0.22499999999999998	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	9	0.22499999999999998	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	8	0.2	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	8	0.2	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	8	0.2	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	8	0.2	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	8	0.2	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	8	0.2	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	8	0.2	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	8	0.2	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	8	0.2	No Hit
GTTGCTAAGCTTCCAACCAAACTCTCCTGCTCCTCAATTTCCTGGTATTC	8	0.2	No Hit
AGGGCATCTGCACGGTCATCCAGCTCCGACAACTTCTGGTCCCGCTCCAG	8	0.2	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	8	0.2	No Hit
TCCAGCCCTCCGTCACTCCCATCTTTCTTCTTCCCATCTCCATCCACCTC	7	0.17500000000000002	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	7	0.17500000000000002	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	7	0.17500000000000002	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	7	0.17500000000000002	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	7	0.17500000000000002	No Hit
GCAATCAGCAGAGTCAACACCAAGTCACCCAGAACAATCCCAGCCAGTAC	7	0.17500000000000002	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	7	0.17500000000000002	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	7	0.17500000000000002	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
TACTTAGTGACAGCCTTGGTGCCCTCCGACACGGCGTGCTTGGCCAGCTC	6	0.15	No Hit
CATCAGAAGACTCGGACACCAGCTTCCCATCTCGGGTTTCAATCTTCTTC	6	0.15	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	6	0.15	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	6	0.15	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	6	0.15	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	6	0.15	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	6	0.15	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	6	0.15	No Hit
ATGGCCATGATGGCATTGCGGATGTCCTCCTCATCAGCGTTGGAGCTTAC	6	0.15	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	6	0.15	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	6	0.15	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCT	6	0.15	No Hit
GTCAACCTCATGTGGCAGGTTCCCAATGAAGAGCTGGTGACTGTCAGGGT	6	0.15	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	6	0.15	No Hit
CACTGGTTTGCCTGATTAAGCTGTTCACACCCAGAACGGTAGCAGCTGCA	6	0.15	No Hit
TTGAAAGCAACCCAACAGGACCGGTCACACACTCTCCACCAAGTTTAAAA	6	0.15	No Hit
TGATTTCAAGCTTCCCTATGGCCCTCATTCTCACTGGCCCGTCATCGATA	6	0.15	No Hit
ATTCCAATCCACTGGCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGGC	6	0.15	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	6	0.15	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	6	0.15	No Hit
TGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTC	5	0.125	No Hit
TTCTACACTGCCTACTGCTTACATTTTCCAAACCCATCAGCATCACTCCT	5	0.125	No Hit
TAAGTACGGTCTTCATAGTGAACCTCCTTGAACAGAAGTTGAAGAGGTCT	5	0.125	No Hit
GGAACAGCGTCATTAGCACCTTGCCTACAGCCACCTCCAAGGCCACCTGC	5	0.125	No Hit
CCCGGTCCAGGATCTTGAAGCTCGTGTCCAGTTTCAGAGGCTGGCTACAG	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
GAAACCTCTCGGACAGCAATCAGCTCCTTTCCATTGTCTACACGTGTGAA	5	0.125	No Hit
CTTTAGTCCAGGCCACTTCAGTCATCAGCAAGCGGAGGTAGTACATGGCA	5	0.125	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	5	0.125	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	5	0.125	No Hit
AACAGCTCCTTGAGAACACGGTCCTGACTCTGCACGGTGTGCACGATGAT	5	0.125	No Hit
ACCTTGTAGGCCTCTGACTGCTGGTATGCCCACAGCTCCTTCAAGTACTG	5	0.125	No Hit
AGCAAACAACCCAATCTTGATGACTTGCCACAAAAGTCCCAGGACCAGGT	5	0.125	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	5	0.125	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	5	0.125	No Hit
TGAAAGGTCTTTGTCGAACACCTCTCCTGAGCGCAGCGCCGAGACCAGGT	5	0.125	No Hit
GAGCTAGCACTTCTCCCAGCTCCGATCGGTTTGTCTCCTGCGGCGCAGAC	5	0.125	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	5	0.125	No Hit
GAGCCTGATCGGAAGTCCATCGTCTCTTCTTCCTGCCTCTTGAGTCCATC	5	0.125	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	5	0.125	No Hit
TTGGCACTGTCAAAGGTCATTTGGTACCCAGCAAGCCAGCCCTCGTAACC	5	0.125	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGCGG	20	6.952051E-4	44.000004	1
CCAATCC	20	6.952051E-4	44.000004	4
CCGTGCA	20	6.952051E-4	44.000004	8
GTGCAGA	20	6.952051E-4	44.000004	10
GGCCGTG	20	6.952051E-4	44.000004	6
GCTGCTC	20	6.952051E-4	44.000004	35
AGCGGCC	20	6.952051E-4	44.000004	3
CGTGCAG	20	6.952051E-4	44.000004	9
CGGCCGT	20	6.952051E-4	44.000004	5
CTGCTGG	20	6.952051E-4	44.000004	21
GCCGTGC	20	6.952051E-4	44.000004	7
CAGCGGC	20	6.952051E-4	44.000004	2
ATTCCAA	20	6.952051E-4	44.000004	1
CTTCATT	30	1.9515464E-6	44.0	19
TTCATTC	30	1.9515464E-6	44.0	20
ATCCACT	25	3.6958358E-5	44.0	7
TGTCTGC	25	3.6958358E-5	44.0	18
CCTGTCT	25	3.6958358E-5	44.0	16
TCAGGCC	30	1.9515464E-6	44.0	9
CATTCTT	30	1.9515464E-6	44.0	15
>>END_MODULE
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230052 spots for SRR25943836.sra
Written 230052 spots for SRR25943836.sra
Read 230057 spots for SRR25943836.sra
Written 230057 spots for SRR25943836.sra
SRR ids: ['SRR25943836.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_dwjedoap
SRR25943836.sra spots: 1840421
blocks: [[1, 230052], [230053, 460104], [460105, 690156], [690157, 920208], [920209, 1150260], [1150261, 1380312], [1380313, 1610364], [1610365, 1840421]]
SRR25943836 file size 317388
SRR25943836 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943836 SRR25943836_1.fastq
Input file:	SRR25943836_1.fastq
trimmed:	SRR25943836-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:39:14 2026 >> started

Tue May  5 05:39:14 2026 >> done (0.556s)
1840421 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
1840421 (100.00%) reads available; of these:
     31 ( 0.00%) trimmed reads available after processing
1840390 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      1	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	      3	  0.00%
 47	      2	  0.00%
 48	     10	  0.00%
 49	     14	  0.00%
 50	1840390	100.00%
1840421 reads passed initial QC


criterion=sequence-density
sequence-density=6.21
sequence-density-rank=1
fanout-score=13.79
fanout-score-rank=1
prefix-density=6.38
prefix-fanout=13.4
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC


criterion=fanout-score
sequence-density=6.21
sequence-density-rank=1
fanout-score=13.79
fanout-score-rank=1
prefix-density=6.38
prefix-fanout=13.4
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC -o SRR25943836 -
Input file:	STDIN
trimmed:	SRR25943836-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Tue May  5 05:39:17 2026 >> started

Tue May  5 05:39:17 2026 >> done (0.529s)
1314587 reads processed; of these:
  51735 ( 3.94%) short reads filtered out after trimming by size control
   3805 ( 0.29%) empty reads filtered out after trimming by size control
1259047 (95.78%) reads available; of these:
  86028 ( 6.83%) trimmed reads available after processing
1173019 (93.17%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    243	  0.02%
 19	    261	  0.02%
 20	    318	  0.03%
 21	    410	  0.03%
 22	    414	  0.03%
 23	    793	  0.06%
 24	   1411	  0.11%
 25	   2404	  0.19%
 26	   1474	  0.12%
 27	   1996	  0.16%
 28	   1933	  0.15%
 29	   2065	  0.16%
 30	   1908	  0.15%
 31	   2554	  0.20%
 32	   1786	  0.14%
 33	   1551	  0.12%
 34	   1814	  0.14%
 35	   1615	  0.13%
 36	   1532	  0.12%
 37	   1533	  0.12%
 38	   2121	  0.17%
 39	   2388	  0.19%
 40	   1564	  0.12%
 41	   1471	  0.12%
 42	   2183	  0.17%
 43	   1755	  0.14%
 44	   2067	  0.16%
 45	   2980	  0.24%
 46	  20478	  1.63%
 47	  21012	  1.67%
 48	      7	  0.00%
 49	     10	  0.00%
 50	1172996	 93.17%


criterion=sequence-density
sequence-density=6.32
sequence-density-rank=1
fanout-score=1.52
fanout-score-rank=8
prefix-density=0.08
prefix-fanout=1.5
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCT


criterion=fanout-score
sequence-density=1.84
sequence-density-rank=3
fanout-score=14.00
fanout-score-rank=1
prefix-density=1.95
prefix-fanout=13.2
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
                                 Started job on |	May 05 05:39:23
                             Started mapping on |	May 05 05:39:23
                                    Finished on |	May 05 05:39:26
       Mapping speed, Million of reads per hour |	2141.86

                          Number of input reads |	1784881
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1400335
                        Uniquely mapped reads % |	78.46%
                          Average mapped length |	49.15
                       Number of splices: Total |	1927
            Number of splices: Annotated (sjdb) |	1142
                       Number of splices: GT/AG |	1556
                       Number of splices: GC/AG |	91
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	279
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.10%
                        Deletion average length |	1.31
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	162885
             % of reads mapped to multiple loci |	9.13%
        Number of reads mapped to too many loci |	126299
             % of reads mapped to too many loci |	7.08%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.33%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	221661	221661	221661
N_multimapping	162885	162885	162885
N_noFeature	2418	1351281	2555
N_ambiguous	60671	36	12702
UnstrandedReadsAssigned:1337246 PositiveStrandReadsAssigned:49018 NegativeStrandReadsAssigned:1385078
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943836 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943836-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,784,881 reads, 1,556,916 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,050 rounds

 109283 SRR25943836.ke.tsv
  52636 SRR25943836.se.tsv
 161919 total
==> SRR25943836.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943836.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943836 completed mapping pipeline successfully
