Starting /dee2/code/volunteer_pipeline.sh SRR25943828
    current disk space = 1266351132
    free memory = 194658444 
SRR25943828 SRAfilesize
9c02d16cd21e1d205664c67bb6eb6e94  SRR25943828.sra
SRR25943828.sra file validated
SRR25943828 is single end
SRR25943828 is conventional basespace
SRR25943828 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943828_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.336	32.0	32.0	32.0	32.0	32.0
2	31.496	32.0	32.0	32.0	32.0	32.0
3	31.53	32.0	32.0	32.0	32.0	32.0
4	31.7185	32.0	32.0	32.0	32.0	32.0
5	31.6255	32.0	32.0	32.0	32.0	32.0
6	35.06375	36.0	36.0	36.0	36.0	36.0
7	35.106	36.0	36.0	36.0	36.0	36.0
8	35.107	36.0	36.0	36.0	36.0	36.0
9	35.129	36.0	36.0	36.0	36.0	36.0
10	34.986	36.0	36.0	36.0	32.0	36.0
11	35.10875	36.0	36.0	36.0	36.0	36.0
12	35.146	36.0	36.0	36.0	36.0	36.0
13	35.08	36.0	36.0	36.0	36.0	36.0
14	35.0985	36.0	36.0	36.0	36.0	36.0
15	34.906	36.0	36.0	36.0	36.0	36.0
16	34.9655	36.0	36.0	36.0	36.0	36.0
17	35.077	36.0	36.0	36.0	36.0	36.0
18	34.9815	36.0	36.0	36.0	36.0	36.0
19	35.0875	36.0	36.0	36.0	36.0	36.0
20	35.039	36.0	36.0	36.0	36.0	36.0
21	35.01275	36.0	36.0	36.0	36.0	36.0
22	35.10675	36.0	36.0	36.0	36.0	36.0
23	35.0065	36.0	36.0	36.0	36.0	36.0
24	35.05075	36.0	36.0	36.0	36.0	36.0
25	35.23	36.0	36.0	36.0	36.0	36.0
26	34.78625	36.0	36.0	36.0	32.0	36.0
27	34.95325	36.0	36.0	36.0	36.0	36.0
28	34.797	36.0	36.0	36.0	32.0	36.0
29	34.9135	36.0	36.0	36.0	36.0	36.0
30	34.85675	36.0	36.0	36.0	32.0	36.0
31	34.89825	36.0	36.0	36.0	32.0	36.0
32	34.8905	36.0	36.0	36.0	32.0	36.0
33	34.796	36.0	36.0	36.0	32.0	36.0
34	34.82125	36.0	36.0	36.0	32.0	36.0
35	34.9305	36.0	36.0	36.0	32.0	36.0
36	35.05925	36.0	36.0	36.0	36.0	36.0
37	34.83425	36.0	36.0	36.0	32.0	36.0
38	34.92725	36.0	36.0	36.0	32.0	36.0
39	35.02925	36.0	36.0	36.0	36.0	36.0
40	34.564	36.0	36.0	36.0	32.0	36.0
41	34.77475	36.0	36.0	36.0	32.0	36.0
42	34.747	36.0	36.0	36.0	32.0	36.0
43	34.6785	36.0	36.0	36.0	32.0	36.0
44	34.678	36.0	36.0	36.0	32.0	36.0
45	34.56475	36.0	36.0	36.0	32.0	36.0
46	34.4315	36.0	36.0	36.0	32.0	36.0
47	34.5325	36.0	36.0	36.0	32.0	36.0
48	34.308	36.0	36.0	36.0	32.0	36.0
49	34.51975	36.0	36.0	36.0	32.0	36.0
50	34.16175	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	-0.32112676056338074
11101	2	0.1760563380281681
11101	3	-0.018309859154928887
11101	4	-0.09154929577464799
11101	5	0.15884194053208134
11101	6	0.1143974960876335
11101	7	-0.21236306729264243
11101	8	0.046948356807504865
11101	9	0.03348982785602317
11101	10	-0.24976525821595885
11101	11	-0.050547730829421766
11101	12	0.25242566510172537
11101	13	0.0186228482003159
11101	14	-0.4730829420970295
11101	15	-0.25899843505477094
11101	16	0.3042253521126739
11101	17	-0.31658841940532056
11101	18	-0.23302034428795082
11101	19	-0.09702660406885855
11101	20	-0.1330203442879494
11101	21	-0.49295774647887214
11101	22	-0.29843505477308696
11101	23	-0.06776212832550499
11101	24	-0.5076682316118948
11101	25	-0.19076682316119076
11101	26	-0.026447574334895307
11101	27	-0.12644757433490383
11101	28	0.17934272300469445
11101	29	0.049452269170579655
11101	30	-0.23067292644757487
11101	31	-0.4161189358372468
11101	32	-0.48372456964006005
11101	33	-0.16384976525821315
11101	34	-0.1195618153364606
11101	35	-0.19061032863849903
11101	36	0.019248826291075716
11101	37	-0.5153364632237825
11101	38	-0.4471048513302023
11101	39	0.6948356807511686
11101	40	-0.20829420970265744
11101	41	0.0017214397496090328
11101	42	-0.4181533646322393
11101	43	0.16447574334898007
11101	44	0.4363067292644729
11101	45	-0.015336463223789565
11101	46	-0.1195618153364606
11101	47	0.5378716744913916
11101	48	-0.3503912363067272
11101	49	-0.22018779342722894
11101	50	-0.2892018779342749
11102	1	0.32112676056338074
11102	2	-0.17605633802817167
11102	3	0.01830985915493244
11102	4	0.09154929577464799
11102	5	-0.15884194053208134
11102	6	-0.1143974960876406
11102	7	0.21236306729264953
11102	8	-0.04694835680751197
11102	9	-0.03348982785602317
11102	10	0.24976525821596596
11102	11	0.050547730829421766
11102	12	-0.25242566510171827
11102	13	-0.0186228482003159
11102	14	0.4730829420970224
11102	15	0.25899843505477804
11102	16	-0.3042253521126739
11102	17	0.31658841940532056
11102	18	0.23302034428795082
11102	19	0.09702660406885855
11102	20	0.1330203442879494
11102	21	0.49295774647887214
11102	22	0.29843505477307986
11102	23	0.0677621283255121
11102	24	0.5076682316118948
11102	25	0.19076682316119076
11102	26	0.026447574334895307
11102	27	0.12644757433489673
11102	28	-0.17934272300469445
11102	29	-0.04945226917057255
11102	30	0.23067292644757487
11102	31	0.4161189358372468
11102	32	0.48372456964006005
11102	33	0.16384976525821315
11102	34	0.1195618153364677
11102	35	0.19061032863849903
11102	36	-0.01924882629108282
11102	37	0.5153364632237896
11102	38	0.4471048513302023
11102	39	-0.6948356807511757
11102	40	0.20829420970266455
11102	41	-0.0017214397496090328
11102	42	0.4181533646322322
11102	43	-0.16447574334898718
11102	44	-0.4363067292644729
11102	45	0.015336463223789565
11102	46	0.1195618153364677
11102	47	-0.5378716744913916
11102	48	0.3503912363067272
11102	49	0.22018779342722894
11102	50	0.2892018779342749
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	2.0
25	4.0
26	18.0
27	22.0
28	36.0
29	68.0
30	93.0
31	119.0
32	190.0
33	292.0
34	614.0
35	2541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.375	20.7	23.724999999999998	28.199999999999996
2	23.425	19.7	26.35	30.525000000000002
3	26.724999999999998	24.625	25.3	23.35
4	27.975	20.599999999999998	26.125	25.3
5	16.55	27.85	26.450000000000003	29.15
6	31.681681681681685	23.123123123123122	21.646646646646648	23.54854854854855
7	30.475	18.85	29.575000000000003	21.099999999999998
8	21.475	18.099999999999998	20.825	39.6
9	23.175	18.7	28.725	29.4
10	26.150000000000002	18.425	24.3	31.125000000000004
11	26.275	25.424999999999997	19.125	29.175
12	32.074999999999996	18.25	21.875	27.800000000000004
13	32.074999999999996	26.450000000000003	20.200000000000003	21.275
14	23.724999999999998	19.25	24.325	32.7
15	17.2	26.3	23.3	33.2
16	18.099999999999998	28.299999999999997	29.275000000000002	24.325
17	19.175	20.775	26.5	33.550000000000004
18	23.025000000000002	13.55	40.975	22.45
19	23.875	22.725	27.950000000000003	25.45
20	27.0	14.149999999999999	24.75	34.1
21	18.925	22.125	28.15	30.8
22	19.475	19.5	29.975	31.05
23	26.025	17.375	25.0	31.6
24	24.15	15.5	24.6	35.75
25	1.925	6.9750000000000005	77.375	13.725000000000001
26	17.2	19.900000000000002	20.474999999999998	42.425000000000004
27	27.3	24.3	22.7	25.7
28	18.0	20.599999999999998	28.95	32.45
29	34.949999999999996	20.45	16.425	28.175
30	29.975	19.05	24.575	26.400000000000002
31	27.1	20.9	19.75	32.25
32	19.7	18.625	25.0	36.675000000000004
33	30.5	21.725	20.175	27.6
34	25.6	17.724999999999998	32.475	24.2
35	27.325	18.975	23.45	30.25
36	30.325000000000003	12.875	24.75	32.05
37	21.7	31.3	24.05	22.95
38	29.375	11.85	24.075	34.699999999999996
39	31.574999999999996	20.150000000000002	20.200000000000003	28.075
40	21.425	27.025	23.75	27.800000000000004
41	24.65	25.025	18.55	31.775
42	29.45	19.025	21.3	30.225
43	24.625	22.675	26.05	26.650000000000002
44	27.224999999999998	19.025	22.225	31.525
45	16.325	25.324999999999996	21.95	36.4
46	21.075	23.025000000000002	33.25	22.650000000000002
47	26.35	17.825	17.424999999999997	38.4
48	23.575	26.450000000000003	26.275	23.7
49	28.575	17.175	30.2	24.05
50	19.575	15.049999999999999	22.3	43.075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	1.0
30	2.0
31	1.0
32	0.0
33	1.5
34	3.0
35	2.0
36	1.0
37	7.0
38	13.0
39	26.0
40	39.0
41	124.0
42	209.0
43	158.0
44	107.0
45	158.0
46	209.0
47	214.0
48	219.0
49	401.0
50	583.0
51	543.5
52	504.0
53	398.5
54	293.0
55	431.0
56	569.0
57	488.5
58	408.0
59	410.0
60	412.0
61	290.5
62	169.0
63	137.5
64	106.0
65	79.5
66	53.0
67	38.0
68	23.0
69	27.5
70	32.0
71	36.0
72	40.0
73	21.5
74	3.0
75	2.5
76	2.0
77	1.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.925000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73903966597078	26.85
2	8.768267223382047	6.3
3	4.940848990953375	5.325
4	2.8531663187195546	4.1000000000000005
5	1.6701461377870561	3.0
6	1.1134307585247043	2.4
7	0.9046624913013221	2.275
8	0.3479471120389701	1.0
9	0.41753653444676403	1.35
>10	3.8274182324286707	30.9
>50	0.20876826722338201	5.225
>100	0.20876826722338201	11.275
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	231	5.775	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	115	2.875	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	105	2.625	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	95	2.375	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	61	1.525	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	53	1.325	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	50	1.25	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	49	1.225	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	46	1.15	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	45	1.125	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	44	1.0999999999999999	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	43	1.075	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	43	1.075	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	37	0.9249999999999999	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	36	0.8999999999999999	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	34	0.8500000000000001	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	34	0.8500000000000001	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	33	0.8250000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	31	0.775	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	31	0.775	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	30	0.75	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	30	0.75	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	27	0.675	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	26	0.65	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	25	0.625	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	25	0.625	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	24	0.6	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	24	0.6	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTC	24	0.6	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	23	0.575	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	20	0.5	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	20	0.5	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	19	0.475	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	18	0.44999999999999996	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	18	0.44999999999999996	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	17	0.42500000000000004	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	17	0.42500000000000004	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	15	0.375	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	15	0.375	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	14	0.35000000000000003	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	14	0.35000000000000003	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	14	0.35000000000000003	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	14	0.35000000000000003	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTAT	14	0.35000000000000003	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	13	0.325	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	13	0.325	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	12	0.3	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	12	0.3	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	12	0.3	No Hit
AGAGGCAGCACTTGCTGGAGAAGGTCAGGCGTCCTTGCGTCAGACTGGCT	12	0.3	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	12	0.3	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	12	0.3	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	12	0.3	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	11	0.27499999999999997	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	11	0.27499999999999997	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	11	0.27499999999999997	No Hit
TGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTC	10	0.25	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	10	0.25	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	10	0.25	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	10	0.25	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	10	0.25	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	9	0.22499999999999998	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	9	0.22499999999999998	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	9	0.22499999999999998	No Hit
TTCTTCTCAAGCAGCTCACGAATCTGCTCCTTTAGGACCTCCACCTCCTC	9	0.22499999999999998	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	9	0.22499999999999998	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	9	0.22499999999999998	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	8	0.2	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	8	0.2	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	8	0.2	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	8	0.2	No Hit
CCTGGCTCAGTGCAGTTCCGCAAATGTAAGTCCTCCTTGGTGAAGCACAG	8	0.2	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	7	0.17500000000000002	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	7	0.17500000000000002	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	7	0.17500000000000002	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	7	0.17500000000000002	No Hit
TCTTCCACACTGATCACAGAGACCTTGGAGGTCAGGTCCACGCCCAGGTC	7	0.17500000000000002	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	7	0.17500000000000002	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	7	0.17500000000000002	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
TCTTTACCACTGATCCACAATCAACCCGTTTTCTACCAACAGTATAGGAG	7	0.17500000000000002	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	7	0.17500000000000002	No Hit
CACTGGCACTGAGCCAAGGCAGCTTTGATGGCGGTCACCAGTTTCCGGAA	7	0.17500000000000002	No Hit
CTCCTTCTTCGAGACTTTCACAATTTTTATGCTGCTGCCCGAAGTCTTCT	7	0.17500000000000002	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	7	0.17500000000000002	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	6	0.15	No Hit
ATGGTTCCAGAAGCCAAGAGGTCCCCAGGTCTCAGGTTGCATCCATTAAC	6	0.15	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	6	0.15	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	6	0.15	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	6	0.15	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	6	0.15	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	6	0.15	No Hit
AACTCAGTAAACTCCTTGCCACACTTCTCATTGATGGAGACCCGGATCTC	6	0.15	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	6	0.15	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	6	0.15	No Hit
AGATATGAATGCAGCTCACTCTCCTGGTGGATGCGCCGTTCCTCGATACT	6	0.15	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	6	0.15	No Hit
CAGTTTCTCAAACATCTCTTTGGCTGCCTGGATATTCTGTGTGAGGTAAT	5	0.125	No Hit
CAGCTGCTGCCCGCCGACATGTCTCCTGCACGCCGCCCACGACCCTCCAG	5	0.125	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	5	0.125	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	5	0.125	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	5	0.125	No Hit
AGGTGGGTCCCACCGATTTGCCAGCGCTCTGGGCGCTCACCCCAGTGAAT	5	0.125	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	5	0.125	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	5	0.125	No Hit
ATTCTCCCAGAAGACCTTGAGCAGTTCCTCAAAGCTGATGTGCTCTGGCC	5	0.125	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	5	0.125	No Hit
GACTTCGCTGAACTCGCTGTACTTTTCAAAGCTCCGTTGTCTGGATCTCA	5	0.125	No Hit
GTGGAGACTCCTCACAATGCACCGCCTCGAATTGCTCTTGCCCTTCCTAC	5	0.125	No Hit
CCTGAGAGACAGAGCAGGCCTGTTCTTCATGTACTTGTTACCCAGCCATA	5	0.125	No Hit
GAACGCCGCAGGTGAGAGGCCATTTCATAACATTTCTTGTTGATCAAACC	5	0.125	No Hit
CGCCTTGCAACGCGAGTCTGTGTTTTTGCAGGAACATTTACACGTCTGCG	5	0.125	No Hit
CTGCCCGAGATGCTCATGTGCTCCTGCTCACTCAACATCTCTGGCCGTTC	5	0.125	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	5	0.125	No Hit
AACAGCTCCTTGAGAACACGGTCCTGACTCTGCACGGTGTGCACGATGAT	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	5	0.125	No Hit
CCAGTACCAGCTACTCCGCTCCTCCGAGTCGAAGTTGCCCGCCATGGCCG	5	0.125	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	5	0.125	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	5	0.125	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTGCCCACTGGCGATGCATGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375197 spots for SRR25943828.sra
Written 375197 spots for SRR25943828.sra
Read 375204 spots for SRR25943828.sra
Written 375204 spots for SRR25943828.sra
SRR ids: ['SRR25943828.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bd9e5oot
SRR25943828.sra spots: 3001583
blocks: [[1, 375197], [375198, 750394], [750395, 1125591], [1125592, 1500788], [1500789, 1875985], [1875986, 2251182], [2251183, 2626379], [2626380, 3001583]]
SRR25943828 file size 518320
SRR25943828 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943828 SRR25943828_1.fastq
Input file:	SRR25943828_1.fastq
trimmed:	SRR25943828-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:40:58 2026 >> started

Tue May  5 05:40:59 2026 >> done (0.988s)
3001583 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3001583 (100.00%) reads available; of these:
     64 ( 0.00%) trimmed reads available after processing
3001519 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      2	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	     10	  0.00%
 47	      4	  0.00%
 48	     17	  0.00%
 49	     30	  0.00%
 50	3001519	100.00%
3001583 reads passed initial QC


criterion=sequence-density
sequence-density=6.39
sequence-density-rank=1
fanout-score=1.43
fanout-score-rank=8
prefix-density=0.09
prefix-fanout=1.4
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGT


criterion=fanout-score
sequence-density=0.50
sequence-density-rank=38
fanout-score=18.02
fanout-score-rank=1
prefix-density=8.95
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:41:05
                             Started mapping on |	May 05 05:41:05
                                    Finished on |	May 05 05:41:10
       Mapping speed, Million of reads per hour |	2161.14

                          Number of input reads |	3001583
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2260749
                        Uniquely mapped reads % |	75.32%
                          Average mapped length |	49.61
                       Number of splices: Total |	2017
            Number of splices: Annotated (sjdb) |	1352
                       Number of splices: GT/AG |	1874
                       Number of splices: GC/AG |	18
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	125
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.09%
                        Deletion average length |	1.19
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	313819
             % of reads mapped to multiple loci |	10.46%
        Number of reads mapped to too many loci |	234811
             % of reads mapped to too many loci |	7.82%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.40%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	427015	427015	427015
N_multimapping	313819	313819	313819
N_noFeature	5533	2196277	5563
N_ambiguous	82327	16	19283
UnstrandedReadsAssigned:2172889 PositiveStrandReadsAssigned:64456 NegativeStrandReadsAssigned:2235903
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943828 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943828-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,001,583 reads, 2,653,986 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,150 rounds

 109283 SRR25943828.ke.tsv
  52636 SRR25943828.se.tsv
 161919 total
==> SRR25943828.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943828.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943828 completed mapping pipeline successfully
