Starting /dee2/code/volunteer_pipeline.sh SRR25943808
    current disk space = 1263926340
    free memory = 194152136 
SRR25943808 SRAfilesize
3176e097e52bd356f12814f613e2e0a6  SRR25943808.sra
SRR25943808.sra file validated
SRR25943808 is single end
SRR25943808 is conventional basespace
SRR25943808 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39575	32.0	32.0	32.0	32.0	32.0
2	31.567	32.0	32.0	32.0	32.0	32.0
3	31.63275	32.0	32.0	32.0	32.0	32.0
4	31.681	32.0	32.0	32.0	32.0	32.0
5	31.66875	32.0	32.0	32.0	32.0	32.0
6	35.19575	36.0	36.0	36.0	36.0	36.0
7	35.227	36.0	36.0	36.0	36.0	36.0
8	35.019	36.0	36.0	36.0	32.0	36.0
9	35.087	36.0	36.0	36.0	36.0	36.0
10	35.156	36.0	36.0	36.0	36.0	36.0
11	35.0915	36.0	36.0	36.0	36.0	36.0
12	35.25925	36.0	36.0	36.0	36.0	36.0
13	35.24225	36.0	36.0	36.0	36.0	36.0
14	35.08875	36.0	36.0	36.0	36.0	36.0
15	35.19475	36.0	36.0	36.0	36.0	36.0
16	35.04925	36.0	36.0	36.0	36.0	36.0
17	35.042	36.0	36.0	36.0	36.0	36.0
18	35.08775	36.0	36.0	36.0	36.0	36.0
19	35.11775	36.0	36.0	36.0	36.0	36.0
20	35.04475	36.0	36.0	36.0	36.0	36.0
21	35.12925	36.0	36.0	36.0	36.0	36.0
22	35.01275	36.0	36.0	36.0	36.0	36.0
23	35.21125	36.0	36.0	36.0	36.0	36.0
24	35.069	36.0	36.0	36.0	36.0	36.0
25	35.0725	36.0	36.0	36.0	36.0	36.0
26	34.95	36.0	36.0	36.0	36.0	36.0
27	35.03875	36.0	36.0	36.0	36.0	36.0
28	34.86875	36.0	36.0	36.0	32.0	36.0
29	35.0035	36.0	36.0	36.0	36.0	36.0
30	34.922	36.0	36.0	36.0	36.0	36.0
31	35.0125	36.0	36.0	36.0	36.0	36.0
32	34.982	36.0	36.0	36.0	32.0	36.0
33	34.944	36.0	36.0	36.0	32.0	36.0
34	34.92	36.0	36.0	36.0	32.0	36.0
35	34.99775	36.0	36.0	36.0	36.0	36.0
36	34.9985	36.0	36.0	36.0	36.0	36.0
37	34.8465	36.0	36.0	36.0	32.0	36.0
38	34.9765	36.0	36.0	36.0	32.0	36.0
39	35.021	36.0	36.0	36.0	36.0	36.0
40	34.80625	36.0	36.0	36.0	32.0	36.0
41	34.699	36.0	36.0	36.0	32.0	36.0
42	34.9275	36.0	36.0	36.0	36.0	36.0
43	34.68375	36.0	36.0	36.0	32.0	36.0
44	34.844	36.0	36.0	36.0	32.0	36.0
45	34.5	36.0	36.0	36.0	32.0	36.0
46	34.474	36.0	36.0	36.0	32.0	36.0
47	34.39725	36.0	36.0	36.0	32.0	36.0
48	34.2765	36.0	36.0	36.0	32.0	36.0
49	34.521	36.0	36.0	36.0	32.0	36.0
50	34.0695	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.1666240409207127
11101	2	-0.08810741687979373
11101	3	-0.19194373401534648
11101	4	0.1335038363171357
11101	5	-0.004475703324807512
11101	6	0.2258312020460309
11101	7	0.3461636828644501
11101	8	-0.02340153452685456
11101	9	0.1606138107416868
11101	10	0.24641943734015825
11101	11	-0.04156010230178708
11101	12	-0.024040920716117853
11101	13	-0.001918158567775663
11101	14	0.026342710997447227
11101	15	0.23657289002557746
11101	16	0.11317135549872148
11101	17	-0.04769820971866778
11101	18	0.07122762148337358
11101	19	0.03823529411764781
11101	20	-0.05920716112532176
11101	21	0.21675191815857175
11101	22	0.27685421994884507
11101	23	0.1268542199488536
11101	24	0.022634271099740033
11101	25	0.1251918158567733
11101	26	0.08350383631713498
11101	27	0.10063938618926471
11101	28	0.3588235294117652
11101	29	0.1890025575447538
11101	30	0.2407928388746754
11101	31	0.058312020460355996
11101	32	0.2710997442455252
11101	33	0.25664961636828565
11101	34	0.17544757033248004
11101	35	0.19897698209718584
11101	36	-0.051534526854226215
11101	37	-0.005115089514063698
11101	38	-0.08043478260869108
11101	39	-0.19194373401534648
11101	40	0.020460358056261896
11101	41	-0.025703324808183936
11101	42	0.27877237851662784
11101	43	0.007928388746805126
11101	44	-0.07327365728900048
11101	45	-0.024040920716110747
11101	46	-0.077749360613808
11101	47	0.3107416879795437
11101	48	0.40255754475703753
11101	49	-0.21265984654731795
11101	50	0.5181585677749396
11102	1	-0.16662404092071625
11102	2	0.08810741687979728
11102	3	0.19194373401534648
11102	4	-0.13350383631713214
11102	5	0.004475703324807512
11102	6	-0.22583120204603802
11102	7	-0.3461636828644501
11102	8	0.02340153452685456
11102	9	-0.1606138107416868
11102	10	-0.24641943734015115
11102	11	0.04156010230179419
11102	12	0.024040920716110747
11102	13	0.0019181585677685575
11102	14	-0.026342710997440122
11102	15	-0.23657289002557036
11102	16	-0.11317135549871438
11102	17	0.04769820971867489
11102	18	-0.07122762148338069
11102	19	-0.03823529411764781
11102	20	0.05920716112532176
11102	21	-0.21675191815856465
11102	22	-0.2768542199488522
11102	23	-0.1268542199488465
11102	24	-0.02263427109974714
11102	25	-0.1251918158567804
11102	26	-0.08350383631713498
11102	27	-0.1006393861892576
11102	28	-0.3588235294117652
11102	29	-0.18900255754476092
11102	30	-0.2407928388746825
11102	31	-0.0583120204603631
11102	32	-0.2710997442455181
11102	33	-0.25664961636828565
11102	34	-0.17544757033248004
11102	35	-0.19897698209718584
11102	36	0.05153452685421911
11102	37	0.005115089514070803
11102	38	0.08043478260869819
11102	39	0.19194373401533937
11102	40	-0.020460358056269
11102	41	0.025703324808183936
11102	42	-0.27877237851662073
11102	43	-0.007928388746805126
11102	44	0.07327365728900759
11102	45	0.024040920716110747
11102	46	0.077749360613808
11102	47	-0.3107416879795366
11102	48	-0.4025575447570304
11102	49	0.21265984654731085
11102	50	-0.5181585677749325
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	6.0
26	18.0
27	14.0
28	39.0
29	58.0
30	83.0
31	102.0
32	186.0
33	287.0
34	631.0
35	2574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.0	20.325	24.15	25.525
2	24.8	21.65	22.175	31.374999999999996
3	26.150000000000002	22.900000000000002	28.275	22.675
4	23.0	20.375	29.5	27.125
5	17.224999999999998	23.025000000000002	29.2	30.55
6	31.23280820205051	24.90622655663916	23.1807951987997	20.68017004251063
7	29.975	18.525	31.5	20.0
8	23.375	18.3	22.775000000000002	35.55
9	27.6	17.1	25.124999999999996	30.175
10	24.15	17.2	30.4	28.249999999999996
11	23.0	22.475	22.1	32.425
12	31.125000000000004	17.9	20.75	30.225
13	30.099999999999998	29.625	19.825	20.45
14	23.575	21.425	26.075	28.925
15	19.625	29.7	22.325	28.349999999999998
16	18.725	27.575	29.9	23.799999999999997
17	19.175	18.025	24.625	38.175
18	22.275	18.3	37.724999999999994	21.7
19	20.525	26.174999999999997	29.675	23.625
20	27.0	14.549999999999999	22.7	35.75
21	20.75	21.55	29.95	27.750000000000004
22	21.45	18.35	31.05	29.15
23	27.250000000000004	14.549999999999999	26.5	31.7
24	29.599999999999998	20.05	20.575	29.775000000000002
25	2.325	5.8500000000000005	81.15	10.674999999999999
26	16.45	25.674999999999997	20.65	37.225
27	27.35	24.625	19.325	28.7
28	16.85	27.400000000000002	26.650000000000002	29.099999999999998
29	30.775000000000002	21.65	17.95	29.625
30	26.625	20.525	28.849999999999998	24.0
31	27.825	23.400000000000002	19.3	29.475
32	25.8	17.8	21.975	34.425
33	29.125	24.925	21.075	24.875
34	25.224999999999998	18.65	32.675	23.45
35	22.725	19.275000000000002	24.825	33.175
36	25.0	13.350000000000001	31.4	30.25
37	21.8	32.025	25.074999999999996	21.099999999999998
38	26.0	15.55	24.075	34.375
39	26.674999999999997	25.775	23.400000000000002	24.15
40	20.175	29.225	24.45	26.150000000000002
41	22.575	25.6	22.6	29.225
42	23.5	20.525	25.4	30.575000000000003
43	21.275	23.724999999999998	30.9	24.099999999999998
44	21.8	18.15	26.400000000000002	33.650000000000006
45	15.425	23.75	21.525	39.300000000000004
46	19.425	29.9	30.349999999999998	20.325
47	28.075	16.725	18.85	36.35
48	23.0	30.675	22.625	23.7
49	25.75	18.9	34.625	20.724999999999998
50	17.575	15.875	19.35	47.199999999999996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	2.0
30	4.0
31	4.0
32	4.0
33	2.5
34	1.0
35	3.5
36	6.0
37	15.5
38	25.0
39	36.0
40	47.0
41	184.5
42	322.0
43	270.5
44	219.0
45	281.0
46	343.0
47	268.0
48	193.0
49	389.0
50	585.0
51	564.0
52	543.0
53	416.5
54	290.0
55	451.5
56	613.0
57	456.5
58	300.0
59	268.5
60	237.0
61	156.5
62	76.0
63	79.5
64	83.0
65	71.0
66	59.0
67	40.0
68	21.0
69	17.5
70	14.0
71	14.0
72	14.0
73	7.5
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.85
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73361227336123	26.075
2	11.01813110181311	7.9
3	5.230125523012552	5.625
4	2.0223152022315203	2.9000000000000004
5	1.3249651324965133	2.375
6	1.3249651324965133	2.85
7	0.9762900976290098	2.45
8	0.8368200836820083	2.4
9	0.4881450488145049	1.575
>10	3.486750348675035	24.9
>50	0.20920502092050208	5.050000000000001
>100	0.3486750348675035	15.9
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	199	4.9750000000000005	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	120	3.0	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	108	2.7	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	108	2.7	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	101	2.5250000000000004	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	76	1.9	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	66	1.6500000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	60	1.5	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	47	1.175	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	44	1.0999999999999999	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	38	0.95	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	36	0.8999999999999999	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	34	0.8500000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	32	0.8	No Hit
AGCTGAGCTCTCGACTCACAGATATTACTGGTGGATAGAGCCTCAGGGCC	31	0.775	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	31	0.775	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	28	0.7000000000000001	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	27	0.675	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	27	0.675	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	26	0.65	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	25	0.625	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	24	0.6	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	24	0.6	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	24	0.6	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	23	0.575	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	22	0.5499999999999999	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTAT	22	0.5499999999999999	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	20	0.5	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	19	0.475	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	19	0.475	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	18	0.44999999999999996	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	17	0.42500000000000004	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	16	0.4	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	16	0.4	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	16	0.4	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	16	0.4	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	15	0.375	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	15	0.375	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	14	0.35000000000000003	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	14	0.35000000000000003	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	14	0.35000000000000003	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	14	0.35000000000000003	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	14	0.35000000000000003	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	13	0.325	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	13	0.325	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	13	0.325	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	13	0.325	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	13	0.325	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	12	0.3	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	12	0.3	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	12	0.3	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	12	0.3	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	11	0.27499999999999997	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	10	0.25	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	10	0.25	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	10	0.25	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	10	0.25	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	10	0.25	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	9	0.22499999999999998	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	9	0.22499999999999998	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	9	0.22499999999999998	No Hit
TGGAGAAACTCGTGTGAGGATTAATCAGGTAGAAAGAGACTATGCAGGGT	9	0.22499999999999998	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	9	0.22499999999999998	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTA	9	0.22499999999999998	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	9	0.22499999999999998	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	8	0.2	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	8	0.2	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	8	0.2	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	8	0.2	No Hit
TCTTCCACACTGATCACAGAGACCTTGGAGGTCAGGTCCACGCCCAGGTC	8	0.2	No Hit
TGGAAGGACACTGGTCAGCAATCCTCAACACAGCACGCCACTTCCCAAAG	8	0.2	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	8	0.2	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	8	0.2	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	8	0.2	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	8	0.2	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	8	0.2	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	8	0.2	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	7	0.17500000000000002	No Hit
AGCTCTTGGTGATGATGGCCCGGCCCCCAAGGTGGCGAGGCTCCAGTGCT	7	0.17500000000000002	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	7	0.17500000000000002	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	7	0.17500000000000002	No Hit
CTAGCACATTCTCTGATGGCACCTTCACTCCATCAAAGTACACCTCTGAC	7	0.17500000000000002	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	7	0.17500000000000002	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	7	0.17500000000000002	No Hit
CCTGAGAGACAGAGCAGGCCTGTTCTTCATGTACTTGTTACCCAGCCATA	7	0.17500000000000002	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	7	0.17500000000000002	No Hit
CCAGCTGCCCTTGAGTGATGTACTTTTTCCACCAGAGGTAGGGACGCATG	7	0.17500000000000002	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	7	0.17500000000000002	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	7	0.17500000000000002	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	7	0.17500000000000002	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	7	0.17500000000000002	No Hit
CTTGCGGATGCCATCTTCTTCCACTTCGTAGTAGTCAGGCCCACAGGCTC	6	0.15	No Hit
AGGCCAGTCGGAGTGCATGGACCTTTTGCATTTCCTCACCGGAGAAGCTA	6	0.15	No Hit
TGGTCAGCCTCCTGGATGGTCCTGTCCGCAATACTGCCCAGCTGCTCATC	6	0.15	No Hit
ATTGAGTGTGTTCCAGATGTAGTCTCGTAACTTCTCATCTGACACGTCTT	6	0.15	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	6	0.15	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	6	0.15	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	6	0.15	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	6	0.15	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	6	0.15	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	6	0.15	No Hit
TTCTGGATGTCAGCCTCTCGCTGCTTGATCAGGTTGTCTGCGGTGGCCTT	6	0.15	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	6	0.15	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	6	0.15	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	6	0.15	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	6	0.15	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	6	0.15	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
ACTGGTGAACTCGTAGGCAGGCACTTCCAGGTTGAGAGGTGCAGGGCCCT	6	0.15	No Hit
AAACAGGCATTGGTGATCTCTGCCACTGACAGCTGCTCATGGTAGGCCTT	5	0.125	No Hit
GTCTCCTCACTGAATTTGTAGCCCTCCAGCAGCAATACGAGGATGTACAG	5	0.125	No Hit
CCTGGTCCTTCAGCCATCGTCTCCTTAGCTCCCGCAGCTGCTGCAGCCGC	5	0.125	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	5	0.125	No Hit
AGGCATGCTTGACAATTCGCACAGTCATGAGCTTCTTGCCGTTGTTACGA	5	0.125	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	5	0.125	No Hit
GTGAACGCAGTGAAGGCCTCCTCCTCGTGCTTGGGTACCAAACGCCAATC	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	5	0.125	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTT	5	0.125	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	5	0.125	No Hit
ATGGCCATGATGGCATTGCGGATGTCCTCCTCATCAGCGTTGGAGCTTAC	5	0.125	No Hit
GTCAATCCCTTCCCTCTTATAGCCTTCGAGAATCTCCTCACTGTCCCAGG	5	0.125	No Hit
GCTCTTACATCACTGCTCTGTGTCTTCTCCAATATCCAGTCCATGTACTC	5	0.125	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	5	0.125	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGC	5	0.125	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	5	0.125	No Hit
GCCATGAGGTCCACCACCCTGTTGCTGTAGCCGTATTCATTGTCATACCA	5	0.125	No Hit
CTGTGTTCTCCCTACAACTGTGTGTGTGATCCTCCTCAATGCTTCCCTCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTATC	20	6.952051E-4	44.000004	42
ACTATCT	20	6.952051E-4	44.000004	43
CTATCTC	20	6.952051E-4	44.000004	44
CTACAAA	30	1.9515464E-6	44.0	35
GAGAATA	25	3.6958358E-5	44.0	29
AAACTTC	30	1.9515464E-6	44.0	39
ACAAACT	30	1.9515464E-6	44.0	37
TCCCATA	25	3.6958358E-5	44.0	20
GTGTCCA	35	1.02281774E-7	44.0	7
CAGTTCT	35	1.02281774E-7	44.0	12
CTCCTTT	25	3.6958358E-5	44.0	5
CCAGTTC	35	1.02281774E-7	44.0	11
ATAAGAG	25	3.6958358E-5	44.0	35
TTTCTGG	25	3.6958358E-5	44.0	9
TGTCCAG	35	1.02281774E-7	44.0	8
TCTGGCT	25	3.6958358E-5	44.0	11
GTTCTAC	35	1.02281774E-7	44.0	14
TATAAGA	25	3.6958358E-5	44.0	34
TGATCCC	25	3.6958358E-5	44.0	17
AAGAGAC	25	3.6958358E-5	44.0	37
>>END_MODULE
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238684 spots for SRR25943808.sra
Written 238684 spots for SRR25943808.sra
Read 238685 spots for SRR25943808.sra
Written 238685 spots for SRR25943808.sra
SRR ids: ['SRR25943808.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_s9ehxjry
SRR25943808.sra spots: 1909473
blocks: [[1, 238684], [238685, 477368], [477369, 716052], [716053, 954736], [954737, 1193420], [1193421, 1432104], [1432105, 1670788], [1670789, 1909473]]
SRR25943808 file size 329336
SRR25943808 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943808 SRR25943808_1.fastq
Input file:	SRR25943808_1.fastq
trimmed:	SRR25943808-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:46:45 2026 >> started

Tue May  5 05:46:45 2026 >> done (0.603s)
1909473 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
1909473 (100.00%) reads available; of these:
     43 ( 0.00%) trimmed reads available after processing
1909430 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 43	      2	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      4	  0.00%
 47	      6	  0.00%
 48	     13	  0.00%
 49	     18	  0.00%
 50	1909430	100.00%
1909473 reads passed initial QC


criterion=sequence-density
sequence-density=5.75
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=17
prefix-density=0.00
prefix-fanout=1.0
sequence=GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATCCCTG


criterion=fanout-score
sequence-density=5.38
sequence-density-rank=2
fanout-score=12.79
fanout-score-rank=1
prefix-density=5.51
prefix-fanout=12.5
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:46:52
                             Started mapping on |	May 05 05:46:52
                                    Finished on |	May 05 05:46:55
       Mapping speed, Million of reads per hour |	2291.37

                          Number of input reads |	1909473
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1452519
                        Uniquely mapped reads % |	76.07%
                          Average mapped length |	49.56
                       Number of splices: Total |	1077
            Number of splices: Annotated (sjdb) |	499
                       Number of splices: GT/AG |	934
                       Number of splices: GC/AG |	12
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	131
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.09%
                        Deletion average length |	1.22
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	171468
             % of reads mapped to multiple loci |	8.98%
        Number of reads mapped to too many loci |	124325
             % of reads mapped to too many loci |	6.51%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.44%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	285486	285486	285486
N_multimapping	171468	171468	171468
N_noFeature	998	1418317	1024
N_ambiguous	47002	4	13135
UnstrandedReadsAssigned:1404519 PositiveStrandReadsAssigned:34198 NegativeStrandReadsAssigned:1438360
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943808 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943808-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,909,473 reads, 1,661,206 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,079 rounds

 109283 SRR25943808.ke.tsv
  52636 SRR25943808.se.tsv
 161919 total
==> SRR25943808.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943808.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943808 completed mapping pipeline successfully
