Starting /dee2/code/volunteer_pipeline.sh SRR25943810
    current disk space = 1262900252
    free memory = 193573296 
SRR25943810 SRAfilesize
904498f0cd68bb6378005781472fba23  SRR25943810.sra
SRR25943810.sra file validated
SRR25943810 is single end
SRR25943810 is conventional basespace
SRR25943810 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3625	32.0	32.0	32.0	32.0	32.0
2	31.43525	32.0	32.0	32.0	32.0	32.0
3	31.6015	32.0	32.0	32.0	32.0	32.0
4	31.6945	32.0	32.0	32.0	32.0	32.0
5	31.578	32.0	32.0	32.0	32.0	32.0
6	35.16075	36.0	36.0	36.0	36.0	36.0
7	35.16625	36.0	36.0	36.0	36.0	36.0
8	34.9835	36.0	36.0	36.0	36.0	36.0
9	35.08725	36.0	36.0	36.0	36.0	36.0
10	35.07175	36.0	36.0	36.0	36.0	36.0
11	35.0355	36.0	36.0	36.0	36.0	36.0
12	35.2175	36.0	36.0	36.0	36.0	36.0
13	35.25725	36.0	36.0	36.0	36.0	36.0
14	35.059	36.0	36.0	36.0	36.0	36.0
15	35.13625	36.0	36.0	36.0	36.0	36.0
16	35.04	36.0	36.0	36.0	36.0	36.0
17	34.92875	36.0	36.0	36.0	36.0	36.0
18	35.0135	36.0	36.0	36.0	36.0	36.0
19	35.07825	36.0	36.0	36.0	36.0	36.0
20	35.1225	36.0	36.0	36.0	36.0	36.0
21	35.04675	36.0	36.0	36.0	36.0	36.0
22	35.042	36.0	36.0	36.0	36.0	36.0
23	35.16525	36.0	36.0	36.0	36.0	36.0
24	35.00325	36.0	36.0	36.0	36.0	36.0
25	35.2505	36.0	36.0	36.0	36.0	36.0
26	34.89	36.0	36.0	36.0	32.0	36.0
27	34.983	36.0	36.0	36.0	36.0	36.0
28	34.8025	36.0	36.0	36.0	32.0	36.0
29	35.06475	36.0	36.0	36.0	36.0	36.0
30	34.8625	36.0	36.0	36.0	32.0	36.0
31	35.1595	36.0	36.0	36.0	36.0	36.0
32	34.9475	36.0	36.0	36.0	32.0	36.0
33	34.79325	36.0	36.0	36.0	32.0	36.0
34	34.7855	36.0	36.0	36.0	32.0	36.0
35	34.975	36.0	36.0	36.0	36.0	36.0
36	35.035	36.0	36.0	36.0	36.0	36.0
37	34.817	36.0	36.0	36.0	32.0	36.0
38	34.835	36.0	36.0	36.0	32.0	36.0
39	34.90425	36.0	36.0	36.0	36.0	36.0
40	34.63975	36.0	36.0	36.0	32.0	36.0
41	34.67	36.0	36.0	36.0	32.0	36.0
42	34.82125	36.0	36.0	36.0	32.0	36.0
43	34.81825	36.0	36.0	36.0	32.0	36.0
44	34.75175	36.0	36.0	36.0	32.0	36.0
45	34.612	36.0	36.0	36.0	32.0	36.0
46	34.47525	36.0	36.0	36.0	32.0	36.0
47	34.529	36.0	36.0	36.0	32.0	36.0
48	34.39375	36.0	36.0	36.0	32.0	36.0
49	34.59075	36.0	36.0	36.0	32.0	36.0
50	34.0845	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	-0.19735836918499317
11101	2	-0.34055727554179427
11101	3	-0.006171846729124297
11101	4	-0.07408226448474409
11101	5	-0.08491817779743371
11101	6	-0.21476820393228735
11101	7	-0.1835873105222987
11101	8	-0.0769772023641977
11101	9	-0.19000040207470903
11101	10	0.25594065377347164
11101	11	-0.5447107072494077
11101	12	-0.27544127698926246
11101	13	-0.12476378111053066
11101	14	-0.24882393148646997
11101	15	-0.08528004503236275
11101	16	-0.0459370351011259
11101	17	-0.4348035865063693
11101	18	0.04050902657714062
11101	19	-0.29315266776567483
11101	20	-0.30047042740541485
11101	21	-0.23006714647581106
11101	22	0.12482409231635216
11101	23	-0.15315025531743487
11101	24	0.31396003377427206
11101	25	-0.2678018575851411
11101	26	-0.19367938562984932
11101	27	-0.1855172691086011
11101	28	-0.06308552128985667
11101	29	-0.1527079731414105
11101	30	-0.1503960435848981
11101	31	-0.09267821961320521
11101	32	0.07382091592618423
11101	33	0.38741908246552725
11101	34	-0.09541232761046814
11101	35	-0.19098548510313407
11101	36	-0.348357524828117
11101	37	-0.3286558642595807
11101	38	0.216436813960037
11101	39	-0.4642153512122533
11101	40	-0.33020385187568024
11101	41	-0.2679827912026056
11101	42	-0.5589843592939587
11101	43	-0.05920550038197092
11101	44	-0.05025933818503603
11101	45	0.027361183707931502
11101	46	-0.20248482167986737
11101	47	-0.22098025813195932
11101	48	0.04668087330625781
11101	49	-0.3293795987294459
11101	50	-0.24399903502070686
11102	1	0.19735836918499672
11102	2	0.3405572755417978
11102	3	0.006171846729120745
11102	4	0.07408226448474053
11102	5	0.08491817779743727
11102	6	0.21476820393229445
11102	7	0.1835873105222916
11102	8	0.0769772023641977
11102	9	0.19000040207470192
11102	10	-0.25594065377347164
11102	11	0.5447107072494077
11102	12	0.27544127698926957
11102	13	0.12476378111053066
11102	14	0.24882393148646997
11102	15	0.08528004503236986
11102	16	0.045937035101118795
11102	17	0.4348035865063764
11102	18	-0.04050902657713351
11102	19	0.29315266776566773
11102	20	0.30047042740540775
11102	21	0.23006714647581816
11102	22	-0.12482409231635216
11102	23	0.15315025531744197
11102	24	-0.31396003377427917
11102	25	0.2678018575851411
11102	26	0.19367938562984932
11102	27	0.1855172691086011
11102	28	0.06308552128985667
11102	29	0.1527079731414105
11102	30	0.1503960435848981
11102	31	0.09267821961320521
11102	32	-0.07382091592617712
11102	33	-0.38741908246552015
11102	34	0.09541232761046814
11102	35	0.19098548510312696
11102	36	0.3483575248281099
11102	37	0.3286558642595807
11102	38	-0.2164368139600299
11102	39	0.4642153512122533
11102	40	0.33020385187568024
11102	41	0.2679827912026056
11102	42	0.5589843592939516
11102	43	0.05920550038197092
11102	44	0.05025933818503603
11102	45	-0.027361183707938608
11102	46	0.20248482167986737
11102	47	0.22098025813195932
11102	48	-0.046680873306264914
11102	49	0.3293795987294459
11102	50	0.24399903502070686
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	4.0
25	9.0
26	10.0
27	20.0
28	41.0
29	60.0
30	89.0
31	115.0
32	182.0
33	274.0
34	592.0
35	2601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.125	19.975	23.400000000000002	26.5
2	24.075	22.7	23.150000000000002	30.075000000000003
3	27.35	22.825	27.675	22.15
4	24.675	19.900000000000002	28.225	27.200000000000003
5	17.8	23.425	30.349999999999998	28.425
6	30.89817363022267	25.268951713785338	23.092319239429575	20.740555416562422
7	30.599999999999998	18.35	30.9	20.150000000000002
8	24.95	17.825	20.825	36.4
9	25.15	17.849999999999998	25.775	31.225
10	22.75	15.950000000000001	30.575000000000003	30.725
11	23.400000000000002	24.75	19.25	32.6
12	33.0	15.950000000000001	19.85	31.2
13	31.05	29.625	19.975	19.35
14	25.275	20.375	24.775	29.575000000000003
15	18.475	29.625	22.15	29.75
16	17.575	28.95	30.049999999999997	23.425
17	19.8	18.475	25.650000000000002	36.075
18	22.875	17.849999999999998	38.375	20.9
19	18.85	25.6	31.324999999999996	24.224999999999998
20	25.474999999999998	15.1	24.075	35.35
21	19.775000000000002	19.8	32.9	27.525
22	20.1	18.3	31.2	30.4
23	29.225	14.575	26.3	29.9
24	30.75	20.424999999999997	21.6	27.224999999999998
25	1.825	4.6	82.675	10.9
26	16.400000000000002	27.1	19.975	36.525
27	28.549999999999997	24.85	19.675	26.924999999999997
28	16.825000000000003	26.025	26.875	30.275000000000002
29	30.55	22.175	16.575	30.7
30	25.424999999999997	22.15	28.449999999999996	23.974999999999998
31	29.7	20.95	18.575	30.775000000000002
32	26.775	20.3	21.175	31.75
33	28.575	25.2	20.3	25.924999999999997
34	21.825	19.45	35.199999999999996	23.525
35	24.474999999999998	19.275000000000002	24.8	31.45
36	24.4	12.7	32.45	30.45
37	21.45	31.3	27.05	20.200000000000003
38	28.000000000000004	14.000000000000002	23.375	34.625
39	26.974999999999998	25.025	23.075000000000003	24.925
40	20.325	28.875	21.8	28.999999999999996
41	24.275	24.0	23.3	28.425
42	24.525	20.7	25.674999999999997	29.099999999999998
43	20.599999999999998	23.575	30.0	25.825
44	22.975	18.65	27.0	31.374999999999996
45	14.924999999999999	24.8	19.6	40.675
46	20.0	30.2	31.25	18.55
47	28.875	15.1	18.2	37.824999999999996
48	22.525000000000002	31.15	24.775	21.55
49	27.200000000000003	17.9	34.0	20.9
50	16.75	16.85	21.725	44.675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	3.0
30	6.0
31	3.0
32	0.0
33	0.5
34	1.0
35	4.0
36	7.0
37	16.5
38	26.0
39	37.0
40	48.0
41	188.0
42	328.0
43	289.0
44	250.0
45	298.5
46	347.0
47	264.0
48	181.0
49	361.0
50	541.0
51	531.5
52	522.0
53	397.5
54	273.0
55	448.5
56	624.0
57	450.5
58	277.0
59	295.0
60	313.0
61	204.0
62	95.0
63	82.0
64	69.0
65	54.0
66	39.0
67	30.5
68	22.0
69	20.5
70	19.0
71	14.5
72	10.0
73	5.0
74	0.0
75	0.0
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0394265232975	24.775
2	12.473118279569892	8.7
3	4.659498207885305	4.875
4	2.7240143369175627	3.8
5	1.6487455197132617	2.875
6	1.6487455197132617	3.45
7	0.9318996415770611	2.275
8	0.7168458781362007	2.0
9	0.14336917562724014	0.44999999999999996
>10	3.4408602150537635	22.95
>50	0.21505376344086022	5.325
>100	0.35842293906810035	18.525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	193	4.825	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	161	4.025	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	144	3.5999999999999996	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	137	3.4250000000000003	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	106	2.65	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	74	1.8499999999999999	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	70	1.7500000000000002	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	69	1.725	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	47	1.175	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	44	1.0999999999999999	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	42	1.05	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	40	1.0	No Hit
AGCTGAGCTCTCGACTCACAGATATTACTGGTGGATAGAGCCTCAGGGCC	31	0.775	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	31	0.775	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	30	0.75	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	30	0.75	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	28	0.7000000000000001	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	28	0.7000000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	25	0.625	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	25	0.625	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	22	0.5499999999999999	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	22	0.5499999999999999	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	21	0.525	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	21	0.525	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	20	0.5	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	20	0.5	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	20	0.5	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	18	0.44999999999999996	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTC	18	0.44999999999999996	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	17	0.42500000000000004	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	16	0.4	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	16	0.4	No Hit
AGAGGCAGCACTTGCTGGAGAAGGTCAGGCGTCCTTGCGTCAGACTGGCT	16	0.4	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	15	0.375	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	14	0.35000000000000003	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	14	0.35000000000000003	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	13	0.325	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	13	0.325	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	13	0.325	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTAT	13	0.325	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	12	0.3	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	12	0.3	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	12	0.3	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	11	0.27499999999999997	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	11	0.27499999999999997	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	11	0.27499999999999997	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	11	0.27499999999999997	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	11	0.27499999999999997	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	11	0.27499999999999997	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	11	0.27499999999999997	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	11	0.27499999999999997	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	11	0.27499999999999997	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	10	0.25	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	10	0.25	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	10	0.25	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	10	0.25	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	9	0.22499999999999998	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	9	0.22499999999999998	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	8	0.2	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	8	0.2	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	8	0.2	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	8	0.2	No Hit
TGGAGAAACTCGTGTGAGGATTAATCAGGTAGAAAGAGACTATGCAGGGT	8	0.2	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	8	0.2	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	8	0.2	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	8	0.2	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	8	0.2	No Hit
CTGTGTTCTCCCTACAACTGTGTGTGTGATCCTCCTCAATGCTTCCCTCT	8	0.2	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	7	0.17500000000000002	No Hit
TTCTACACTGCCTACTGCTTACATTTTCCAAACCCATCAGCATCACTCCT	7	0.17500000000000002	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	7	0.17500000000000002	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	7	0.17500000000000002	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	7	0.17500000000000002	No Hit
AGCTCGTCCTCTGTGGTCTTCTGGTAGACTGGGTTGTCAAAGTTTATGCT	7	0.17500000000000002	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	7	0.17500000000000002	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	7	0.17500000000000002	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	7	0.17500000000000002	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	7	0.17500000000000002	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	7	0.17500000000000002	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	7	0.17500000000000002	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	6	0.15	No Hit
ATTGAGTGTGTTCCAGATGTAGTCTCGTAACTTCTCATCTGACACGTCTT	6	0.15	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	6	0.15	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	6	0.15	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	6	0.15	No Hit
AAGATTGTCCCGGAGTAGCGTTACTTCAATGTGACCCTTAGCAAAGACGC	6	0.15	No Hit
AGGTGGGTCCCACCGATTTGCCAGCGCTCTGGGCGCTCACCCCAGTGAAT	6	0.15	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	6	0.15	No Hit
CTAGCACATTCTCTGATGGCACCTTCACTCCATCAAAGTACACCTCTGAC	6	0.15	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	6	0.15	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	6	0.15	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	6	0.15	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	6	0.15	No Hit
TCAGGAGGAACTTGGTCTCTGTGGTCACATTGTGCATGACCTGCCAGTGC	6	0.15	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
AGCCCTTCGGTGTCCATGGCCAAGTACTGGCCGGTCTCCGTACCCTTTAT	6	0.15	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	6	0.15	No Hit
TTTGGAGCCGAGACTGTTGTTCCATAATGTGGTTGTTCAGAATCAGCGCC	6	0.15	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	6	0.15	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	6	0.15	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	6	0.15	No Hit
AGGCCAGTCGGAGTGCATGGACCTTTTGCATTTCCTCACCGGAGAAGCTA	5	0.125	No Hit
AAACTCTGGTCACAAACTGACTGCTTCAGGCCTTTGGTCATCAGAATGTA	5	0.125	No Hit
TGGTCAGCCTCCTGGATGGTCCTGTCCGCAATACTGCCCAGCTGCTCATC	5	0.125	No Hit
AGGACTATGGCCTTGAGGCAGCTATACTCGGCGGAGTCGACGTGCAGTGC	5	0.125	No Hit
GTCCATGGTGTACACTTTCACTCCCCGGAAGCTGAAGCCCGAACACCGAT	5	0.125	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	5	0.125	No Hit
CCTGGTCCTTCAGCCATCGTCTCCTTAGCTCCCGCAGCTGCTGCAGCCGC	5	0.125	No Hit
GCTCCATTAATCCAAGAGGCATAATCACTGCATAGGAAAGTGGCCAGATT	5	0.125	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	5	0.125	No Hit
GTGGCTCATTCCAGAAGCTGAACTCCAGAAGCAACTGGTTTCTCCTGGAC	5	0.125	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	5	0.125	No Hit
TGGAAGGACACTGGTCAGCAATCCTCAACACAGCACGCCACTTCCCAAAG	5	0.125	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	5	0.125	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	5	0.125	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	5	0.125	No Hit
CTCAAGAGGTATGTTCCTATGAGCTAGCCTGAGCTTCCTTGCAATGCGCT	5	0.125	No Hit
CCAGCTGCCCTTGAGTGATGTACTTTTTCCACCAGAGGTAGGGACGCATG	5	0.125	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	5	0.125	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	5	0.125	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	5	0.125	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	5	0.125	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	5	0.125	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCC	20	6.952051E-4	44.000004	29
ATCTTCT	20	6.952051E-4	44.000004	15
GCCCACT	20	6.952051E-4	44.000004	33
ACAGAGG	20	6.952051E-4	44.000004	3
GCGATGC	20	6.952051E-4	44.000004	41
TTTCTGG	20	6.952051E-4	44.000004	9
GTATCTT	20	6.952051E-4	44.000004	13
GATGCAT	20	6.952051E-4	44.000004	43
GGCGATG	20	6.952051E-4	44.000004	40
GGTTTGC	20	6.952051E-4	44.000004	28
CCCACTG	20	6.952051E-4	44.000004	34
CATCAGG	20	6.952051E-4	44.000004	23
CTGGCGA	20	6.952051E-4	44.000004	38
TGCCCAC	20	6.952051E-4	44.000004	32
TATCTTC	20	6.952051E-4	44.000004	14
CCATCAG	20	6.952051E-4	44.000004	22
CCACAGA	20	6.952051E-4	44.000004	1
CCTTTCT	20	6.952051E-4	44.000004	7
TCCATCA	20	6.952051E-4	44.000004	21
ATGCATG	20	6.952051E-4	44.000004	44
>>END_MODULE
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339022 spots for SRR25943810.sra
Written 339022 spots for SRR25943810.sra
Read 339026 spots for SRR25943810.sra
Written 339026 spots for SRR25943810.sra
SRR ids: ['SRR25943810.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_58yvan_t
SRR25943810.sra spots: 2712180
blocks: [[1, 339022], [339023, 678044], [678045, 1017066], [1017067, 1356088], [1356089, 1695110], [1695111, 2034132], [2034133, 2373154], [2373155, 2712180]]
SRR25943810 file size 468240
SRR25943810 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943810 SRR25943810_1.fastq
Input file:	SRR25943810_1.fastq
trimmed:	SRR25943810-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:47:04 2026 >> started

Tue May  5 05:47:05 2026 >> done (0.871s)
2712180 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2712180 (100.00%) reads available; of these:
     65 ( 0.00%) trimmed reads available after processing
2712115 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      3	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	     13	  0.00%
 47	      5	  0.00%
 48	     21	  0.00%
 49	     21	  0.00%
 50	2712115	100.00%
2712180 reads passed initial QC


criterion=sequence-density
sequence-density=5.79
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=18
prefix-density=0.00
prefix-fanout=1.0
sequence=GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATCCCTGT


criterion=fanout-score
sequence-density=3.40
sequence-density-rank=4
fanout-score=14.73
fanout-score-rank=1
prefix-density=3.50
prefix-fanout=14.3
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGTTTATACTATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:47:11
                             Started mapping on |	May 05 05:47:11
                                    Finished on |	May 05 05:47:16
       Mapping speed, Million of reads per hour |	1952.77

                          Number of input reads |	2712180
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2092468
                        Uniquely mapped reads % |	77.15%
                          Average mapped length |	49.57
                       Number of splices: Total |	1224
            Number of splices: Annotated (sjdb) |	740
                       Number of splices: GT/AG |	1124
                       Number of splices: GC/AG |	4
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	94
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.17
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	229198
             % of reads mapped to multiple loci |	8.45%
        Number of reads mapped to too many loci |	245415
             % of reads mapped to too many loci |	9.05%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.35%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	390514	390514	390514
N_multimapping	229198	229198	229198
N_noFeature	4008	2040851	4035
N_ambiguous	71682	16	20596
UnstrandedReadsAssigned:2016778 PositiveStrandReadsAssigned:51601 NegativeStrandReadsAssigned:2067837
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943810 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943810-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,712,180 reads, 2,440,527 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,141 rounds

 109283 SRR25943810.ke.tsv
  52636 SRR25943810.se.tsv
 161919 total
==> SRR25943810.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943810.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943810 completed mapping pipeline successfully
