Starting /dee2/code/volunteer_pipeline.sh SRR25943852
    current disk space = 489485629440
    free memory = 278414316 
SRR25943852 SRAfilesize
f986c3474a9422b386875f97050b4cc3  SRR25943852.sra
SRR25943852.sra file validated
SRR25943852 is single end
SRR25943852 is conventional basespace
SRR25943852 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338	32.0	32.0	32.0	32.0	32.0
2	31.3125	32.0	32.0	32.0	32.0	32.0
3	31.43825	32.0	32.0	32.0	32.0	32.0
4	31.5525	32.0	32.0	32.0	32.0	32.0
5	31.54625	32.0	32.0	32.0	32.0	32.0
6	34.96875	36.0	36.0	36.0	36.0	36.0
7	34.9415	36.0	36.0	36.0	36.0	36.0
8	34.90425	36.0	36.0	36.0	32.0	36.0
9	34.95525	36.0	36.0	36.0	32.0	36.0
10	35.06625	36.0	36.0	36.0	32.0	36.0
11	34.89425	36.0	36.0	36.0	32.0	36.0
12	34.98	36.0	36.0	36.0	36.0	36.0
13	34.91225	36.0	36.0	36.0	36.0	36.0
14	34.9115	36.0	36.0	36.0	36.0	36.0
15	34.7295	36.0	36.0	36.0	32.0	36.0
16	35.0275	36.0	36.0	36.0	36.0	36.0
17	34.73275	36.0	36.0	36.0	32.0	36.0
18	34.8485	36.0	36.0	36.0	32.0	36.0
19	34.8595	36.0	36.0	36.0	32.0	36.0
20	34.77125	36.0	36.0	36.0	32.0	36.0
21	34.8755	36.0	36.0	36.0	32.0	36.0
22	34.977	36.0	36.0	36.0	36.0	36.0
23	35.0415	36.0	36.0	36.0	36.0	36.0
24	34.949	36.0	36.0	36.0	36.0	36.0
25	35.0065	36.0	36.0	36.0	36.0	36.0
26	34.707	36.0	36.0	36.0	32.0	36.0
27	34.8665	36.0	36.0	36.0	32.0	36.0
28	34.53725	36.0	36.0	36.0	32.0	36.0
29	34.90475	36.0	36.0	36.0	32.0	36.0
30	34.7305	36.0	36.0	36.0	32.0	36.0
31	34.675	36.0	36.0	36.0	32.0	36.0
32	34.652	36.0	36.0	36.0	32.0	36.0
33	34.57625	36.0	36.0	36.0	32.0	36.0
34	34.72175	36.0	36.0	36.0	32.0	36.0
35	34.82225	36.0	36.0	36.0	32.0	36.0
36	34.93675	36.0	36.0	36.0	32.0	36.0
37	34.846	36.0	36.0	36.0	36.0	36.0
38	34.6885	36.0	36.0	36.0	32.0	36.0
39	34.9045	36.0	36.0	36.0	32.0	36.0
40	34.56125	36.0	36.0	36.0	32.0	36.0
41	34.79975	36.0	36.0	36.0	32.0	36.0
42	34.7265	36.0	36.0	36.0	32.0	36.0
43	34.39225	36.0	36.0	36.0	32.0	36.0
44	34.52275	36.0	36.0	36.0	32.0	36.0
45	34.5575	36.0	36.0	36.0	32.0	36.0
46	34.3005	36.0	36.0	36.0	32.0	36.0
47	34.40625	36.0	36.0	36.0	32.0	36.0
48	34.29775	36.0	36.0	36.0	32.0	36.0
49	34.27	36.0	36.0	36.0	32.0	36.0
50	34.11325	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	5.0
24	6.0
25	11.0
26	13.0
27	23.0
28	49.0
29	58.0
30	129.0
31	157.0
32	189.0
33	294.0
34	591.0
35	2473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.174999999999997	15.7	22.3	35.825
2	25.974999999999998	14.374999999999998	38.05	21.6
3	42.6	21.375	20.05	15.975
4	42.175000000000004	21.349999999999998	16.5	19.975
5	16.475	36.5	23.5	23.525
6	43.41085271317829	18.27956989247312	19.654913728432106	18.65466366591648
7	41.375	16.0	27.85	14.774999999999999
8	17.275	16.150000000000002	17.224999999999998	49.35
9	16.475	17.0	40.325	26.200000000000003
10	24.7	13.475000000000001	21.8	40.025
11	24.2	39.625	15.125	21.05
12	43.775	16.0	14.274999999999999	25.95
13	42.760690172543136	22.43060765191298	15.603900975243812	19.204801200300075
14	18.175	17.5	20.05	44.275
15	14.549999999999999	24.725	22.625	38.1
16	15.45	38.074999999999996	24.0	22.475
17	17.825	19.3	34.675	28.199999999999996
18	18.075	11.75	50.875	19.3
19	18.2	20.150000000000002	25.5	36.15
20	22.075	13.3	33.525	31.1
21	12.875	19.975	42.1	25.05
22	12.65	17.1	32.4	37.85
23	25.424999999999997	26.75	20.65	27.175
24	20.549999999999997	13.725000000000001	33.0	32.725
25	1.25	4.35	82.025	12.375
26	12.1	18.2	14.575	55.125
27	26.025	20.724999999999998	34.775	18.475
28	15.7	20.275000000000002	22.650000000000002	41.375
29	45.475	21.3	11.425	21.8
30	36.225	18.45	18.5	26.825
31	38.175	18.5	13.15	30.175
32	18.375	19.85	21.3	40.475
33	25.6	20.075000000000003	16.85	37.475
34	24.349999999999998	13.875000000000002	37.925	23.849999999999998
35	36.6	22.325	20.075000000000003	21.0
36	42.475	9.925	25.275	22.325
37	22.475	40.275	23.275000000000002	13.975000000000001
38	42.6	11.175	14.7	31.525
39	40.625	21.375	14.825	23.175
40	23.325000000000003	21.9	16.75	38.025
41	18.0	36.6	14.774999999999999	30.625000000000004
42	42.41060265066267	16.954238559639908	20.005001250312578	20.630157539384847
43	23.849999999999998	22.0	18.325	35.825
44	38.550000000000004	13.525	19.950000000000003	27.975
45	11.602900725181295	38.45961490372593	13.953488372093023	35.98399599899975
46	17.2	21.475	42.975	18.35
47	23.525	14.45	13.125	48.9
48	17.63381690845423	24.012006003001503	39.44472236118059	18.90945472736368
49	39.20980245061265	14.87871967991998	22.605651412853213	23.305826456614152
50	15.925	16.900000000000002	31.525	35.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	0.5
24	1.0
25	0.5
26	0.0
27	0.5
28	1.0
29	1.5
30	2.0
31	1.5
32	1.0
33	1.5
34	2.0
35	1.5
36	1.0
37	5.0
38	9.0
39	30.5
40	52.0
41	109.0
42	166.0
43	148.5
44	131.0
45	147.0
46	163.0
47	144.0
48	125.0
49	351.5
50	578.0
51	521.5
52	465.0
53	333.0
54	201.0
55	312.0
56	423.0
57	370.0
58	317.0
59	590.5
60	864.0
61	582.5
62	301.0
63	191.0
64	81.0
65	68.0
66	55.0
67	33.5
68	12.0
69	17.5
70	23.0
71	22.0
72	21.0
73	12.0
74	3.0
75	2.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.025
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.025
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.025
43	0.0
44	0.0
45	0.025
46	0.0
47	0.0
48	0.05
49	0.025
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.549999999999997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64273204903678	21.025
2	11.29597197898424	6.45
3	4.816112084063047	4.125
4	2.626970227670753	3.0
5	1.3134851138353765	1.875
6	1.0507880910683012	1.7999999999999998
7	0.7880910683012259	1.575
8	0.43782837127845886	1.0
9	0.43782837127845886	1.125
>10	2.626970227670753	16.675
>50	0.6129597197898424	12.049999999999999
>100	0.2626970227670753	10.925
>500	0.08756567425569177	18.375
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	735	18.375	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	197	4.925	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	122	3.05	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	118	2.9499999999999997	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	99	2.475	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	80	2.0	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	79	1.975	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	66	1.6500000000000001	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	55	1.375	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	52	1.3	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	51	1.275	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	40	1.0	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	39	0.975	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	37	0.9249999999999999	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	36	0.8999999999999999	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	34	0.8500000000000001	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	33	0.8250000000000001	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	33	0.8250000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	33	0.8250000000000001	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	28	0.7000000000000001	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	26	0.65	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	25	0.625	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	23	0.575	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	23	0.575	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	21	0.525	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	20	0.5	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	20	0.5	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	17	0.42500000000000004	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	17	0.42500000000000004	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	16	0.4	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	16	0.4	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	16	0.4	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	14	0.35000000000000003	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	14	0.35000000000000003	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAAT	14	0.35000000000000003	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	14	0.35000000000000003	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	13	0.325	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	13	0.325	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	12	0.3	No Hit
CCCGGTCCAGGATCTTGAAGCTCGTGTCCAGTTTCAGAGGCTGGCTACAG	10	0.25	No Hit
CAGTCATACTCGTAGTCAAACACGTAGCCGCTGCGGTCAAAGAGGTCAGT	10	0.25	No Hit
TCAGCGTTCACTAGGCAGACCATCTGCAACACAATGGTGGCTCCCATACG	9	0.22499999999999998	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	9	0.22499999999999998	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	9	0.22499999999999998	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	9	0.22499999999999998	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	9	0.22499999999999998	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	8	0.2	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	8	0.2	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	8	0.2	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	8	0.2	No Hit
CGACTGGAGCTTCAGCACTTCTGGTCCACCAAACTCAAACACTCGAATAG	8	0.2	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	7	0.17500000000000002	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	7	0.17500000000000002	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	7	0.17500000000000002	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	7	0.17500000000000002	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	7	0.17500000000000002	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	7	0.17500000000000002	No Hit
CTTGGCAGGCAGCCTCAGATTTGCCAAGATATTATCCATTTCAGAATTCA	7	0.17500000000000002	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	6	0.15	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	6	0.15	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	6	0.15	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
AGTAGGTTTCCATCTTGGCAATCTTTTCATCGAAGTACTTCCTGCCATAG	6	0.15	No Hit
ATTAACCTCAAGAAGCGAGCGTCATAGTATAAAGAAGGCTTGACGACAAA	6	0.15	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	6	0.15	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATA	6	0.15	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	6	0.15	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	6	0.15	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	6	0.15	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	5	0.125	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	5	0.125	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	5	0.125	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAATCGG	5	0.125	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	5	0.125	No Hit
TGGAAGGACACTGGTCAGCAATCCTCAACACAGCACGCCACTTCCCAAAG	5	0.125	No Hit
AGCCCGTGCACTCGCGCAATACCATCACCAATGCTTAACACACGCCCAGT	5	0.125	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	5	0.125	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	5	0.125	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGC	5	0.125	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	5	0.125	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	5	0.125	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATGGA	20	6.952051E-4	44.000004	2
GATGGAT	20	6.952051E-4	44.000004	3
CATTGGG	20	6.952051E-4	44.000004	34
GGGCAGG	20	6.952051E-4	44.000004	38
TAAACAT	20	6.952051E-4	44.000004	27
AAACATC	20	6.952051E-4	44.000004	28
TTCGCAG	20	6.952051E-4	44.000004	10
CCTAAAC	20	6.952051E-4	44.000004	25
GCAGGCC	20	6.952051E-4	44.000004	13
GGATTTC	20	6.952051E-4	44.000004	6
ATTGGGC	20	6.952051E-4	44.000004	35
CATCATT	20	6.952051E-4	44.000004	31
ATGGATT	20	6.952051E-4	44.000004	4
ACATCAT	20	6.952051E-4	44.000004	30
GAGCACA	20	6.952051E-4	44.000004	44
TTCCCCT	20	6.952051E-4	44.000004	21
TTGGGCA	20	6.952051E-4	44.000004	36
CCATTCC	20	6.952051E-4	44.000004	18
CGCAGGC	20	6.952051E-4	44.000004	12
TGGATTT	20	6.952051E-4	44.000004	5
>>END_MODULE
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276990 spots for SRR25943852.sra
Written 276990 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
Read 276977 spots for SRR25943852.sra
Written 276977 spots for SRR25943852.sra
SRR ids: ['SRR25943852.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ol_c4p_u
SRR25943852.sra spots: 4431645
blocks: [[1, 276977], [276978, 553954], [553955, 830931], [830932, 1107908], [1107909, 1384885], [1384886, 1661862], [1661863, 1938839], [1938840, 2215816], [2215817, 2492793], [2492794, 2769770], [2769771, 3046747], [3046748, 3323724], [3323725, 3600701], [3600702, 3877678], [3877679, 4154655], [4154656, 4431645]]
SRR25943852 file size 109654
SRR25943852 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25943852 SRR25943852_1.fastq
Input file:	SRR25943852_1.fastq
trimmed:	SRR25943852-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Tue May  5 08:18:38 2026 >> started

Tue May  5 08:18:40 2026 >> done (2.529s)
4431645 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
4431645 (100.00%) reads available; of these:
     93 ( 0.00%) trimmed reads available after processing
4431552 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      2	  0.00%
 44	      2	  0.00%
 45	      0	  0.00%
 46	     19	  0.00%
 47	      8	  0.00%
 48	     22	  0.00%
 49	     39	  0.00%
 50	4431552	100.00%
4431645 reads passed initial QC


criterion=sequence-density
sequence-density=19.64
sequence-density-rank=1
fanout-score=1.73
fanout-score-rank=6
prefix-density=0.05
prefix-fanout=1.7
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGT


criterion=fanout-score
sequence-density=0.78
sequence-density-rank=20
fanout-score=34.15
fanout-score-rank=1
prefix-density=26.25
prefix-fanout=1.0
sequence=CAGCGATCTGTAGCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 08:19:54
                             Started mapping on |	May 05 08:19:54
                                    Finished on |	May 05 08:20:17
       Mapping speed, Million of reads per hour |	693.65

                          Number of input reads |	4431645
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2694522
                        Uniquely mapped reads % |	60.80%
                          Average mapped length |	49.63
                       Number of splices: Total |	1406
            Number of splices: Annotated (sjdb) |	666
                       Number of splices: GT/AG |	1253
                       Number of splices: GC/AG |	36
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	117
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.07%
                        Deletion average length |	1.11
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	511117
             % of reads mapped to multiple loci |	11.53%
        Number of reads mapped to too many loci |	1020445
             % of reads mapped to too many loci |	23.03%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.63%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1226006	1226006	1226006
N_multimapping	511117	511117	511117
N_noFeature	3537	2610687	3586
N_ambiguous	105063	23	22458
UnstrandedReadsAssigned:2585922 PositiveStrandReadsAssigned:83812 NegativeStrandReadsAssigned:2668478
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943852 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943852-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,431,645 reads, 4,039,609 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,158 rounds

 109283 SRR25943852.ke.tsv
  52636 SRR25943852.se.tsv
 161919 total
==> SRR25943852.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943852.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943852 completed mapping pipeline successfully
