Starting /dee2/code/volunteer_pipeline.sh SRR25943841
    current disk space = 1265506696
    free memory = 193099868 
SRR25943841 SRAfilesize
ae1ec776cd1e4c0ab9c546cad10e9ac1  SRR25943841.sra
SRR25943841.sra file validated
SRR25943841 is single end
SRR25943841 is conventional basespace
SRR25943841 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41	32.0	32.0	32.0	32.0	32.0
2	31.50475	32.0	32.0	32.0	32.0	32.0
3	31.50325	32.0	32.0	32.0	32.0	32.0
4	31.58075	32.0	32.0	32.0	32.0	32.0
5	31.58	32.0	32.0	32.0	32.0	32.0
6	35.142	36.0	36.0	36.0	36.0	36.0
7	35.231	36.0	36.0	36.0	36.0	36.0
8	35.07875	36.0	36.0	36.0	36.0	36.0
9	35.0675	36.0	36.0	36.0	36.0	36.0
10	35.095	36.0	36.0	36.0	36.0	36.0
11	34.9555	36.0	36.0	36.0	32.0	36.0
12	35.123	36.0	36.0	36.0	36.0	36.0
13	35.013	36.0	36.0	36.0	36.0	36.0
14	35.04775	36.0	36.0	36.0	36.0	36.0
15	34.941	36.0	36.0	36.0	36.0	36.0
16	35.0355	36.0	36.0	36.0	36.0	36.0
17	35.0	36.0	36.0	36.0	36.0	36.0
18	35.0225	36.0	36.0	36.0	36.0	36.0
19	35.0315	36.0	36.0	36.0	36.0	36.0
20	34.87575	36.0	36.0	36.0	36.0	36.0
21	35.05975	36.0	36.0	36.0	36.0	36.0
22	35.05575	36.0	36.0	36.0	36.0	36.0
23	35.20725	36.0	36.0	36.0	36.0	36.0
24	35.126	36.0	36.0	36.0	36.0	36.0
25	34.85325	36.0	36.0	36.0	36.0	36.0
26	35.01625	36.0	36.0	36.0	36.0	36.0
27	34.8245	36.0	36.0	36.0	32.0	36.0
28	34.6905	36.0	36.0	36.0	32.0	36.0
29	35.007	36.0	36.0	36.0	36.0	36.0
30	34.8985	36.0	36.0	36.0	32.0	36.0
31	34.817	36.0	36.0	36.0	36.0	36.0
32	34.81025	36.0	36.0	36.0	32.0	36.0
33	34.89225	36.0	36.0	36.0	32.0	36.0
34	34.86325	36.0	36.0	36.0	32.0	36.0
35	34.9375	36.0	36.0	36.0	36.0	36.0
36	34.96825	36.0	36.0	36.0	36.0	36.0
37	34.818	36.0	36.0	36.0	32.0	36.0
38	34.78425	36.0	36.0	36.0	32.0	36.0
39	34.98775	36.0	36.0	36.0	36.0	36.0
40	34.5565	36.0	36.0	36.0	32.0	36.0
41	34.86725	36.0	36.0	36.0	36.0	36.0
42	34.92125	36.0	36.0	36.0	32.0	36.0
43	34.59275	36.0	36.0	36.0	32.0	36.0
44	34.76825	36.0	36.0	36.0	32.0	36.0
45	34.7065	36.0	36.0	36.0	32.0	36.0
46	34.65875	36.0	36.0	36.0	32.0	36.0
47	34.6825	36.0	36.0	36.0	32.0	36.0
48	34.3565	36.0	36.0	36.0	32.0	36.0
49	34.55725	36.0	36.0	36.0	32.0	36.0
50	34.22275	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	0.0
24	8.0
25	8.0
26	14.0
27	18.0
28	47.0
29	62.0
30	90.0
31	110.0
32	189.0
33	273.0
34	597.0
35	2582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.125	14.174999999999999	17.9	32.800000000000004
2	22.95	23.75	32.225	21.075
3	45.975	19.650000000000002	15.55	18.825
4	43.85	16.775000000000002	17.8	21.575
5	15.8	30.599999999999998	33.275	20.325
6	33.425	28.275	19.55	18.75
7	34.625	13.55	34.875	16.950000000000003
8	18.275	15.45	15.174999999999999	51.1
9	17.849999999999998	19.1	32.25	30.8
10	22.0	13.025	33.550000000000004	31.424999999999997
11	20.45	44.574999999999996	13.525	21.45
12	48.4	13.8	16.7	21.099999999999998
13	48.449999999999996	18.425	15.25	17.875
14	12.075	19.125	33.550000000000004	35.25
15	13.0	32.574999999999996	19.125	35.3
16	12.375	44.275	23.45	19.900000000000002
17	13.900000000000002	19.2	30.15	36.75
18	17.5	23.175	41.349999999999994	17.974999999999998
19	17.875	18.65	34.675	28.799999999999997
20	31.5	13.475000000000001	29.349999999999998	25.674999999999997
21	13.700000000000001	16.375	46.75	23.175
22	23.75	15.975	28.749999999999996	31.525
23	32.7	22.8	21.725	22.775000000000002
24	18.55	22.900000000000002	31.900000000000002	26.650000000000002
25	1.5	4.2	85.15	9.15
26	11.3	25.674999999999997	18.7	44.324999999999996
27	31.374999999999996	19.375	27.675	21.575
28	15.85	27.500000000000004	25.4	31.25
29	37.25	17.349999999999998	11.275	34.125
30	29.125	14.499999999999998	32.95	23.425
31	42.625	20.3	12.925	24.15
32	27.55	17.299999999999997	19.3	35.85
33	27.800000000000004	25.275	14.424999999999999	32.5
34	20.549999999999997	24.85	36.55	18.05
35	28.175	19.35	31.05	21.425
36	35.725	11.65	33.575	19.05
37	30.075000000000003	32.45	21.2	16.275000000000002
38	43.7	10.625	15.125	30.55
39	32.7	30.825000000000003	16.25	20.225
40	19.650000000000002	21.125	17.825	41.4
41	16.875	29.825000000000003	25.900000000000002	27.400000000000002
42	36.725	15.049999999999999	28.65	19.575
43	20.05	21.65	16.775000000000002	41.525
44	32.125	14.174999999999999	31.574999999999996	22.125
45	10.95	32.550000000000004	16.6	39.900000000000006
46	15.375	28.525	37.45	18.65
47	33.800000000000004	12.375	17.224999999999998	36.6
48	15.975	18.375	43.6	22.05
49	39.725	20.0	21.075	19.2
50	13.675	25.074999999999996	26.650000000000002	34.599999999999994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	0.0
29	9.5
30	19.0
31	9.5
32	0.0
33	0.5
34	1.0
35	5.0
36	9.0
37	17.0
38	25.0
39	55.0
40	85.0
41	154.5
42	224.0
43	366.5
44	509.0
45	358.5
46	208.0
47	175.0
48	142.0
49	323.0
50	504.0
51	490.5
52	477.0
53	332.0
54	187.0
55	267.5
56	348.0
57	308.5
58	269.0
59	466.0
60	663.0
61	435.0
62	207.0
63	132.5
64	58.0
65	43.0
66	28.0
67	23.5
68	19.0
69	12.0
70	5.0
71	7.5
72	10.0
73	6.0
74	2.0
75	1.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.974999999999998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41673182173572	23.474999999999998
2	11.415168100078187	7.3
3	5.08209538702111	4.875
4	2.18921032056294	2.8000000000000003
5	1.2509773260359656	2.0
6	1.09460516028147	2.1
7	1.1727912431587177	2.625
8	0.46911649726348714	1.2
9	0.23455824863174357	0.675
>10	3.2838154808444098	22.75
>50	0.07818608287724785	1.7999999999999998
>100	0.23455824863174357	15.325
>500	0.07818608287724785	13.075000000000001
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	523	13.075000000000001	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	404	10.100000000000001	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	107	2.675	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	102	2.55	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	72	1.7999999999999998	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	47	1.175	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	46	1.15	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	44	1.0999999999999999	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	43	1.075	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	42	1.05	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	41	1.0250000000000001	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	35	0.8750000000000001	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	34	0.8500000000000001	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	30	0.75	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	29	0.7250000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	28	0.7000000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	26	0.65	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	24	0.6	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	24	0.6	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	23	0.575	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	20	0.5	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	20	0.5	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	19	0.475	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	19	0.475	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	18	0.44999999999999996	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	18	0.44999999999999996	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	17	0.42500000000000004	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	17	0.42500000000000004	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	17	0.42500000000000004	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	16	0.4	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	15	0.375	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	15	0.375	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCAT	15	0.375	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	15	0.375	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	14	0.35000000000000003	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	14	0.35000000000000003	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCATCTC	13	0.325	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	13	0.325	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	12	0.3	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	12	0.3	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	12	0.3	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	11	0.27499999999999997	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	11	0.27499999999999997	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	11	0.27499999999999997	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	10	0.25	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	10	0.25	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	10	0.25	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGC	9	0.22499999999999998	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	9	0.22499999999999998	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	8	0.2	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	8	0.2	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	8	0.2	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	8	0.2	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	8	0.2	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	8	0.2	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	7	0.17500000000000002	No Hit
CTTGCGGATGCCATCTTCTTCCACTTCGTAGTAGTCAGGCCCACAGGCTC	7	0.17500000000000002	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	7	0.17500000000000002	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	7	0.17500000000000002	No Hit
AGCACAAAGTCCTCCCGGTACTCCTGGCGGATATCTTTGCGCAGTTTGGC	7	0.17500000000000002	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
TGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAG	7	0.17500000000000002	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCATGAC	7	0.17500000000000002	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	7	0.17500000000000002	No Hit
TAGTAATATCCTGAGTCCTCCACCTTGGCAGGTACAAACCAAAGATGTTC	7	0.17500000000000002	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	7	0.17500000000000002	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	7	0.17500000000000002	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	7	0.17500000000000002	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	7	0.17500000000000002	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAGGTAGCATCTC	7	0.17500000000000002	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	6	0.15	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	6	0.15	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGATTGGACTTCTCAGTGAC	6	0.15	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTCTCAGTGAC	6	0.15	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	6	0.15	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	6	0.15	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	6	0.15	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	6	0.15	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	6	0.15	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTAAG	6	0.15	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	5	0.125	No Hit
CCTGGTCCTTCAGCCATCGTCTCCTTAGCTCCCGCAGCTGCTGCAGCCGC	5	0.125	No Hit
CTAGCACATTCTCTGATGGCACCTTCACTCCATCAAAGTACACCTCTGAC	5	0.125	No Hit
CTCTCCGCACCTTCTGCCAGCTTGTTCACCATACTAGCATTTCCAGAAGC	5	0.125	No Hit
AATCATCTTCCTGTGACCATTTCCAATCCTCCAGAGGCAGTCTCCAGTCT	5	0.125	No Hit
GTGGCTCATTCCAGAAGCTGAACTCCAGAAGCAACTGGTTTCTCCTGGAC	5	0.125	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	5	0.125	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	5	0.125	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	5	0.125	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	5	0.125	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	5	0.125	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	5	0.125	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	5	0.125	No Hit
CTCTTCCAGCAGCACAGCACTTGGTAACGTATCACTGCCAGCTCTTCCGG	5	0.125	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	5	0.125	No Hit
GCCATGAGGTCCACCACCCTGTTGCTGTAGCCGTATTCATTGTCATACCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATAGA	45	2.728484E-10	44.000004	22
CTGGAAT	45	2.728484E-10	44.000004	29
ATAGACT	40	5.315087E-9	44.000004	24
GACTGGA	40	5.315087E-9	44.000004	27
AGACTGG	40	5.315087E-9	44.000004	26
ACTGGAA	40	5.315087E-9	44.000004	28
TAGACTG	40	5.315087E-9	44.000004	25
GATAGAC	40	5.315087E-9	44.000004	23
ATCCTAG	55	0.0	44.0	6
GAGGTAT	50	1.4551915E-11	44.0	1
TCCTAGG	55	0.0	44.0	7
GTATCCT	55	0.0	44.0	4
CTAGGTA	55	0.0	44.0	9
ATGTGGA	55	0.0	44.0	18
TATCCTA	55	0.0	44.0	5
TAACATG	55	0.0	44.0	14
TGTGGAT	55	0.0	44.0	19
AGGTATC	55	0.0	44.0	2
AGGTAAC	55	0.0	44.0	11
GTGGATA	55	0.0	44.0	20
>>END_MODULE
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433093 spots for SRR25943841.sra
Written 433093 spots for SRR25943841.sra
Read 433097 spots for SRR25943841.sra
Written 433097 spots for SRR25943841.sra
SRR ids: ['SRR25943841.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7y7zf5kw
SRR25943841.sra spots: 3464748
blocks: [[1, 433093], [433094, 866186], [866187, 1299279], [1299280, 1732372], [1732373, 2165465], [2165466, 2598558], [2598559, 3031651], [3031652, 3464748]]
SRR25943841 file size 598468
SRR25943841 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943841 SRR25943841_1.fastq
Input file:	SRR25943841_1.fastq
trimmed:	SRR25943841-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:36:24 2026 >> started

Tue May  5 05:36:25 2026 >> done (0.926s)
3464748 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3464748 (100.00%) reads available; of these:
     84 ( 0.00%) trimmed reads available after processing
3464664 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      3	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	     13	  0.00%
 47	      8	  0.00%
 48	     28	  0.00%
 49	     31	  0.00%
 50	3464664	100.00%
3464748 reads passed initial QC


criterion=sequence-density
sequence-density=13.89
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=15
prefix-density=0.04
prefix-fanout=1.0
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTGTG


criterion=fanout-score
sequence-density=1.00
sequence-density-rank=12
fanout-score=18.81
fanout-score-rank=1
prefix-density=18.63
prefix-fanout=1.0
sequence=CAGCGATCTGTAGCCTGTGG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:36:31
                             Started mapping on |	May 05 05:36:31
                                    Finished on |	May 05 05:36:38
       Mapping speed, Million of reads per hour |	1781.87

                          Number of input reads |	3464748
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1931966
                        Uniquely mapped reads % |	55.76%
                          Average mapped length |	49.59
                       Number of splices: Total |	1612
            Number of splices: Annotated (sjdb) |	922
                       Number of splices: GT/AG |	1475
                       Number of splices: GC/AG |	28
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	109
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.14
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	391122
             % of reads mapped to multiple loci |	11.29%
        Number of reads mapped to too many loci |	975909
             % of reads mapped to too many loci |	28.17%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.78%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1141660	1141660	1141660
N_multimapping	391122	391122	391122
N_noFeature	15359	1882009	15387
N_ambiguous	67777	26	18498
UnstrandedReadsAssigned:1848830 PositiveStrandReadsAssigned:49931 NegativeStrandReadsAssigned:1898081
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943841 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943841-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,464,748 reads, 3,165,911 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,112 rounds

 109283 SRR25943841.ke.tsv
  52636 SRR25943841.se.tsv
 161919 total
==> SRR25943841.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943841.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943841 completed mapping pipeline successfully
