Starting /dee2/code/volunteer_pipeline.sh SRR25943825
    current disk space = 1264037772
    free memory = 193877700 
SRR25943825 SRAfilesize
1028ee1ae3df131d282a3a0b6e0bdae2  SRR25943825.sra
SRR25943825.sra file validated
SRR25943825 is single end
SRR25943825 is conventional basespace
SRR25943825 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341	32.0	32.0	32.0	32.0	32.0
2	31.552	32.0	32.0	32.0	32.0	32.0
3	31.57075	32.0	32.0	32.0	32.0	32.0
4	31.579	32.0	32.0	32.0	32.0	32.0
5	31.65925	32.0	32.0	32.0	32.0	32.0
6	35.18125	36.0	36.0	36.0	36.0	36.0
7	35.09775	36.0	36.0	36.0	36.0	36.0
8	35.0335	36.0	36.0	36.0	32.0	36.0
9	35.05075	36.0	36.0	36.0	36.0	36.0
10	35.01	36.0	36.0	36.0	32.0	36.0
11	35.097	36.0	36.0	36.0	36.0	36.0
12	35.18275	36.0	36.0	36.0	36.0	36.0
13	35.17925	36.0	36.0	36.0	36.0	36.0
14	35.07775	36.0	36.0	36.0	36.0	36.0
15	35.12775	36.0	36.0	36.0	36.0	36.0
16	35.091	36.0	36.0	36.0	36.0	36.0
17	35.064	36.0	36.0	36.0	36.0	36.0
18	35.042	36.0	36.0	36.0	36.0	36.0
19	35.10725	36.0	36.0	36.0	36.0	36.0
20	35.04375	36.0	36.0	36.0	36.0	36.0
21	35.01225	36.0	36.0	36.0	36.0	36.0
22	35.18875	36.0	36.0	36.0	36.0	36.0
23	35.15625	36.0	36.0	36.0	36.0	36.0
24	35.05425	36.0	36.0	36.0	36.0	36.0
25	35.14825	36.0	36.0	36.0	36.0	36.0
26	34.8425	36.0	36.0	36.0	32.0	36.0
27	34.96375	36.0	36.0	36.0	36.0	36.0
28	34.81325	36.0	36.0	36.0	32.0	36.0
29	35.0265	36.0	36.0	36.0	36.0	36.0
30	34.93625	36.0	36.0	36.0	36.0	36.0
31	34.993	36.0	36.0	36.0	36.0	36.0
32	34.85325	36.0	36.0	36.0	32.0	36.0
33	34.9495	36.0	36.0	36.0	36.0	36.0
34	34.873	36.0	36.0	36.0	32.0	36.0
35	35.08875	36.0	36.0	36.0	36.0	36.0
36	34.90775	36.0	36.0	36.0	36.0	36.0
37	34.82	36.0	36.0	36.0	32.0	36.0
38	34.899	36.0	36.0	36.0	32.0	36.0
39	34.977	36.0	36.0	36.0	36.0	36.0
40	34.67425	36.0	36.0	36.0	32.0	36.0
41	34.726	36.0	36.0	36.0	32.0	36.0
42	34.8585	36.0	36.0	36.0	32.0	36.0
43	34.7335	36.0	36.0	36.0	32.0	36.0
44	34.65675	36.0	36.0	36.0	32.0	36.0
45	34.7115	36.0	36.0	36.0	32.0	36.0
46	34.56425	36.0	36.0	36.0	32.0	36.0
47	34.694	36.0	36.0	36.0	32.0	36.0
48	34.45325	36.0	36.0	36.0	32.0	36.0
49	34.575	36.0	36.0	36.0	32.0	36.0
50	34.18375	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	1.0
24	0.0
25	11.0
26	13.0
27	28.0
28	42.0
29	48.0
30	89.0
31	116.0
32	187.0
33	268.0
34	573.0
35	2623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.725	19.5	30.025000000000002	21.75
2	31.275	22.025	22.375	24.325
3	36.675000000000004	20.200000000000003	24.099999999999998	19.025
4	22.05	23.35	24.925	29.675
5	12.625	21.175	30.875000000000004	35.325
6	29.739869934967484	22.161080540270135	29.864932466233117	18.234117058529264
7	33.050000000000004	17.724999999999998	31.7	17.525
8	23.175	20.4	26.525	29.9
9	26.25	15.825	29.799999999999997	28.125
10	27.450000000000003	18.125	28.65	25.775
11	25.575	31.8	15.675	26.950000000000003
12	31.125000000000004	25.124999999999996	19.85	23.9
13	30.08252063015754	36.18404601150287	16.27906976744186	17.454363590897724
14	21.25	19.975	25.874999999999996	32.9
15	14.424999999999999	37.375	21.025	27.175
16	16.075	27.925	26.525	29.475
17	24.025	27.175	19.25	29.549999999999997
18	19.525000000000002	16.075	34.525	29.875
19	20.75	33.275	27.325	18.65
20	37.574999999999996	13.725000000000001	17.724999999999998	30.975
21	15.325	19.0	29.575000000000003	36.1
22	20.349999999999998	19.725	35.35	24.575
23	36.225	13.225000000000001	22.675	27.875
24	35.975	16.875	17.875	29.275000000000002
25	1.15	5.075	82.92500000000001	10.85
26	15.775	31.05	16.925	36.25
27	25.7	30.075000000000003	16.225	28.000000000000004
28	14.649999999999999	25.374999999999996	33.324999999999996	26.650000000000002
29	28.4	29.275000000000002	14.274999999999999	28.050000000000004
30	20.65	21.175	26.450000000000003	31.724999999999998
31	31.15	24.075	15.675	29.099999999999998
32	31.025000000000002	20.724999999999998	19.85	28.4
33	29.975	19.75	21.0	29.275000000000002
34	18.925	19.2	31.924999999999997	29.95
35	20.65	18.875	34.75	25.724999999999998
36	27.6	11.85	36.0	24.55
37	20.25	35.4	25.0	19.35
38	26.0	13.525	21.675	38.800000000000004
39	32.45	26.200000000000003	19.35	22.0
40	19.55	32.7	23.175	24.575
41	19.1	29.925	21.0	29.975
42	24.90622655663916	19.179794948737182	30.807701925481368	25.10627656914228
43	32.550000000000004	21.9	24.55	21.0
44	22.875	16.775000000000002	23.75	36.6
45	14.17854463615904	25.18129532383096	18.854713678419603	41.785446361590395
46	19.775000000000002	34.050000000000004	27.250000000000004	18.925
47	24.7	18.875	15.1	41.325
48	23.1807951987997	27.731932983245812	20.505126281570394	28.582145536384097
49	26.506626656664167	26.106526631657918	26.131532883220803	21.255313828457115
50	16.275000000000002	28.125	16.075	39.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	1.0
30	2.0
31	1.0
32	0.0
33	0.5
34	1.0
35	4.0
36	7.0
37	17.5
38	28.0
39	40.5
40	53.0
41	174.5
42	296.0
43	271.0
44	246.0
45	240.5
46	235.0
47	217.0
48	199.0
49	529.5
50	860.0
51	743.0
52	626.0
53	443.0
54	260.0
55	384.5
56	509.0
57	385.0
58	261.0
59	231.0
60	201.0
61	151.0
62	101.0
63	82.0
64	63.0
65	41.0
66	19.0
67	15.0
68	11.0
69	11.0
70	11.0
71	10.0
72	9.0
73	4.5
74	0.0
75	0.5
76	1.0
77	1.0
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.025
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.025
43	0.0
44	0.0
45	0.025
46	0.0
47	0.0
48	0.025
49	0.025
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.324999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84841453982985	23.225
2	9.976798143851507	6.45
3	6.0324825986078885	5.8500000000000005
4	3.7122969837587005	4.8
5	1.6241299303944314	2.625
6	0.8507347254447023	1.6500000000000001
7	0.6187161639597835	1.4000000000000001
8	0.6187161639597835	1.6
9	0.6187161639597835	1.7999999999999998
>10	3.402938901778809	21.675
>50	0.30935808197989173	7.925
>100	0.3866976024748647	21.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	349	8.725	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	147	3.675	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	126	3.15	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	117	2.9250000000000003	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	101	2.5250000000000004	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	98	2.45	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	89	2.225	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	65	1.625	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	65	1.625	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	45	1.125	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	44	1.0999999999999999	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	42	1.05	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	38	0.95	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	33	0.8250000000000001	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	30	0.75	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	30	0.75	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	28	0.7000000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	28	0.7000000000000001	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	26	0.65	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	25	0.625	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	24	0.6	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	23	0.575	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	23	0.575	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	22	0.5499999999999999	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	20	0.5	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	18	0.44999999999999996	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	17	0.42500000000000004	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	17	0.42500000000000004	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	16	0.4	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	16	0.4	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	16	0.4	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	16	0.4	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	16	0.4	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	16	0.4	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	15	0.375	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	14	0.35000000000000003	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	14	0.35000000000000003	No Hit
GATATTTGTCCTCATGGAAGGACGTAGCCTATAAATGCTGTGGCTATGAC	14	0.35000000000000003	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	14	0.35000000000000003	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	14	0.35000000000000003	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	13	0.325	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	13	0.325	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	13	0.325	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	13	0.325	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	12	0.3	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	12	0.3	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	12	0.3	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	12	0.3	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	11	0.27499999999999997	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	11	0.27499999999999997	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	11	0.27499999999999997	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	10	0.25	No Hit
AAGCTCTGTCTTCCCATCTGTGGTTACCACCTGGATTAGGCTTCCTGAGT	10	0.25	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	9	0.22499999999999998	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	9	0.22499999999999998	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	9	0.22499999999999998	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	9	0.22499999999999998	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	9	0.22499999999999998	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	9	0.22499999999999998	No Hit
TCTTTACCACTGATCCACAATCAACCCGTTTTCTACCAACAGTATAGGAG	9	0.22499999999999998	No Hit
GCTCTTACATCACTGCTCTGTGTCTTCTCCAATATCCAGTCCATGTACTC	9	0.22499999999999998	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	8	0.2	No Hit
TTCTTCTCAAGCAGCTCACGAATCTGCTCCTTTAGGACCTCCACCTCCTC	8	0.2	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	8	0.2	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	8	0.2	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTC	8	0.2	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	8	0.2	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	8	0.2	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	7	0.17500000000000002	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTT	7	0.17500000000000002	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	7	0.17500000000000002	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	7	0.17500000000000002	No Hit
CTCCACACAGGTGTCCCTGGCTGATGTGCAGAGTCACCTGCCATGGCCAC	7	0.17500000000000002	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	7	0.17500000000000002	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	7	0.17500000000000002	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	7	0.17500000000000002	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	6	0.15	No Hit
GACACACTTGTACCTCCTGCTGAGACTCCAAATATAGTGACCCTGTCACG	6	0.15	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	6	0.15	No Hit
GGAGGAAGCCCAAGCTGCTGTTGTCAATGTTTGTGACCCAGTTACTAGAA	6	0.15	No Hit
GTGGAGACTCCTCACAATGCACCGCCTCGAATTGCTCTTGCCCTTCCTAC	6	0.15	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	6	0.15	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	6	0.15	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	6	0.15	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	6	0.15	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	6	0.15	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	6	0.15	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	5	0.125	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTACGAATGCCACCAAC	5	0.125	No Hit
GCAGACGCAACTCTCGGTAGGTCCTTTTGGCGTGAATGATGGACTGAAAC	5	0.125	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	5	0.125	No Hit
CACAGACAGTCACAATGCACAGGATGACCAGCACCAGCATCATGAAGCCA	5	0.125	No Hit
CTTTGCTCTATCATCCTAGGTGACTGCTATGCTCAACATTGAGCCTTCTT	5	0.125	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	5	0.125	No Hit
TGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAG	5	0.125	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	5	0.125	No Hit
AATGGTCGAGTTGTCCACTGGCTGTAATGTCAGCTTCTGGTCATAGATGT	5	0.125	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTCTACAAACTTCTCATCC	5	0.125	No Hit
ATGACGAGGACAGAGGAGTGCTCCTTCACAAACTGGTCAAAGTCTTCATC	5	0.125	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	5	0.125	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	5	0.125	No Hit
GACTTCGCTGAACTCGCTGTACTTTTCAAAGCTCCGTTGTCTGGATCTCA	5	0.125	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	5	0.125	No Hit
CACTGGCACTGAGCCAAGGCAGCTTTGATGGCGGTCACCAGTTTCCGGAA	5	0.125	No Hit
AGAGGCAGCACTTGCTGGAGAAGGTCAGGCGTCCTTGCGTCAGACTGGCT	5	0.125	No Hit
AGCTTCAATGGCAGTATATGTGGCTCCTGGAGGAACTTGGTAGCCCTCTG	5	0.125	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	5	0.125	No Hit
TGGTCAGCTCGACTGAAAGCCGCTTCTTGTAATCCTGTGGCTTGTCCTCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGAA	40	5.315087E-9	44.000004	6
TCCCATG	20	6.952051E-4	44.000004	35
GTTGAAA	40	5.315087E-9	44.000004	7
CAGTCCC	20	6.952051E-4	44.000004	32
GCCTTAC	40	5.315087E-9	44.000004	32
CACACAG	40	5.315087E-9	44.000004	14
GAAACAC	40	5.315087E-9	44.000004	10
TTACCAG	20	6.952051E-4	44.000004	42
TGGTAAT	40	5.315087E-9	44.000004	22
CCAGTCC	20	6.952051E-4	44.000004	31
AAACACA	40	5.315087E-9	44.000004	11
GTTCTGT	20	6.952051E-4	44.000004	14
GCTGGTA	40	5.315087E-9	44.000004	20
TACGGCC	40	5.315087E-9	44.000004	28
TTCTGTA	20	6.952051E-4	44.000004	15
CCATGTT	20	6.952051E-4	44.000004	37
ATGGCCA	20	6.952051E-4	44.000004	27
GGTTCTG	20	6.952051E-4	44.000004	13
CCCATGT	20	6.952051E-4	44.000004	36
GAATGCC	40	5.315087E-9	44.000004	39
>>END_MODULE
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
Read 440949 spots for SRR25943825.sra
Written 440949 spots for SRR25943825.sra
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
Read 440947 spots for SRR25943825.sra
Written 440947 spots for SRR25943825.sra
SRR ids: ['SRR25943825.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_3ob3i61j
SRR25943825.sra spots: 3527578
blocks: [[1, 440947], [440948, 881894], [881895, 1322841], [1322842, 1763788], [1763789, 2204735], [2204736, 2645682], [2645683, 3086629], [3086630, 3527578]]
SRR25943825 file size 609336
SRR25943825 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943825 SRR25943825_1.fastq
Input file:	SRR25943825_1.fastq
trimmed:	SRR25943825-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:43:29 2026 >> started

Tue May  5 05:43:30 2026 >> done (1.013s)
3527578 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3527578 (100.00%) reads available; of these:
     74 ( 0.00%) trimmed reads available after processing
3527504 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      3	  0.00%
 44	      1	  0.00%
 45	      0	  0.00%
 46	     15	  0.00%
 47	      8	  0.00%
 48	     24	  0.00%
 49	     22	  0.00%
 50	3527504	100.00%
3527578 reads passed initial QC


criterion=sequence-density
sequence-density=9.60
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=24
prefix-density=0.00
prefix-fanout=1.0
sequence=TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAACCT


criterion=fanout-score
sequence-density=1.18
sequence-density-rank=12
fanout-score=15.10
fanout-score-rank=1
prefix-density=1.24
prefix-fanout=14.4
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGACTTCATTATCTCGTA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    1 (100.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:43:35
                             Started mapping on |	May 05 05:43:35
                                    Finished on |	May 05 05:43:40
       Mapping speed, Million of reads per hour |	2539.86

                          Number of input reads |	3527578
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2735702
                        Uniquely mapped reads % |	77.55%
                          Average mapped length |	49.52
                       Number of splices: Total |	2312
            Number of splices: Annotated (sjdb) |	755
                       Number of splices: GT/AG |	2118
                       Number of splices: GC/AG |	30
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	164
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.09
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	424110
             % of reads mapped to multiple loci |	12.02%
        Number of reads mapped to too many loci |	279970
             % of reads mapped to too many loci |	7.94%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.49%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	367766	367766	367766
N_multimapping	424110	424110	424110
N_noFeature	5259	2687364	5270
N_ambiguous	73438	38	25805
UnstrandedReadsAssigned:2657005 PositiveStrandReadsAssigned:48300 NegativeStrandReadsAssigned:2704627
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943825 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943825-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,527,578 reads, 3,288,757 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,160 rounds

 109283 SRR25943825.ke.tsv
  52636 SRR25943825.se.tsv
 161919 total
==> SRR25943825.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943825.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943825 completed mapping pipeline successfully
