Starting /dee2/code/volunteer_pipeline.sh SRR25943834
    current disk space = 1266439456
    free memory = 194678524 
SRR25943834 SRAfilesize
fd39f2407d42da188933fa857671aaba  SRR25943834.sra
SRR25943834.sra file validated
SRR25943834 is single end
SRR25943834 is conventional basespace
SRR25943834 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943834_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4955	32.0	32.0	32.0	32.0	32.0
2	31.57575	32.0	32.0	32.0	32.0	32.0
3	31.521	32.0	32.0	32.0	32.0	32.0
4	31.69375	32.0	32.0	32.0	32.0	32.0
5	31.65175	32.0	32.0	32.0	32.0	32.0
6	35.094	36.0	36.0	36.0	36.0	36.0
7	35.094	36.0	36.0	36.0	36.0	36.0
8	34.994	36.0	36.0	36.0	32.0	36.0
9	34.95975	36.0	36.0	36.0	32.0	36.0
10	35.01775	36.0	36.0	36.0	36.0	36.0
11	35.082	36.0	36.0	36.0	36.0	36.0
12	35.1175	36.0	36.0	36.0	36.0	36.0
13	35.16075	36.0	36.0	36.0	36.0	36.0
14	35.01475	36.0	36.0	36.0	36.0	36.0
15	35.02875	36.0	36.0	36.0	36.0	36.0
16	34.82375	36.0	36.0	36.0	32.0	36.0
17	35.003	36.0	36.0	36.0	36.0	36.0
18	34.89775	36.0	36.0	36.0	36.0	36.0
19	35.02975	36.0	36.0	36.0	36.0	36.0
20	34.98875	36.0	36.0	36.0	36.0	36.0
21	35.06875	36.0	36.0	36.0	36.0	36.0
22	34.99175	36.0	36.0	36.0	36.0	36.0
23	34.98575	36.0	36.0	36.0	36.0	36.0
24	34.94725	36.0	36.0	36.0	36.0	36.0
25	35.19725	36.0	36.0	36.0	36.0	36.0
26	34.79675	36.0	36.0	36.0	32.0	36.0
27	34.75875	36.0	36.0	36.0	32.0	36.0
28	34.7455	36.0	36.0	36.0	32.0	36.0
29	34.92625	36.0	36.0	36.0	36.0	36.0
30	34.87825	36.0	36.0	36.0	36.0	36.0
31	35.04925	36.0	36.0	36.0	36.0	36.0
32	34.767	36.0	36.0	36.0	32.0	36.0
33	34.85675	36.0	36.0	36.0	32.0	36.0
34	34.82925	36.0	36.0	36.0	32.0	36.0
35	34.99875	36.0	36.0	36.0	36.0	36.0
36	34.892	36.0	36.0	36.0	32.0	36.0
37	34.71725	36.0	36.0	36.0	32.0	36.0
38	34.765	36.0	36.0	36.0	32.0	36.0
39	34.806	36.0	36.0	36.0	32.0	36.0
40	34.4775	36.0	36.0	36.0	32.0	36.0
41	34.68575	36.0	36.0	36.0	32.0	36.0
42	34.748	36.0	36.0	36.0	32.0	36.0
43	34.41225	36.0	36.0	36.0	32.0	36.0
44	34.64825	36.0	36.0	36.0	32.0	36.0
45	34.5335	36.0	36.0	36.0	32.0	36.0
46	34.24275	36.0	36.0	36.0	32.0	36.0
47	34.16	36.0	36.0	36.0	32.0	36.0
48	34.15975	36.0	36.0	36.0	32.0	36.0
49	34.21675	36.0	36.0	36.0	32.0	36.0
50	33.9565	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	-0.055765199161424306
11101	2	0.08965758211041219
11101	3	0.15471698113207566
11101	4	-0.12389937106918225
11101	5	-0.07875611460517362
11101	6	0.131306778476592
11101	7	-0.062124388539480435
11101	8	-0.034241788958766506
11101	9	0.3635220125786134
11101	10	-0.15883997204752376
11101	11	0.32851153039832326
11101	12	0.07931516422082296
11101	13	0.13508036338225082
11101	14	0.11201956673654934
11101	15	0.435359888190078
11101	16	0.6844164919636668
11101	17	-0.11313766596785513
11101	18	0.08106219426974093
11101	19	0.6082459818308834
11101	20	0.2621243885394833
11101	21	0.07064989517819953
11101	22	-0.009783368273936333
11101	23	0.10083857442347721
11101	24	0.322571628232005
11101	25	0.31698113207547607
11101	26	-0.10034940600978359
11101	27	0.047798742138368766
11101	28	-0.18238993710691886
11101	29	0.509084556254372
11101	30	0.17973445143255873
11101	31	0.40475192173305885
11101	32	0.5742138364779876
11101	33	0.11264849755416151
11101	34	-0.17784765897973642
11101	35	0.16079664570230534
11101	36	0.3429769392033535
11101	37	0.059049615653385956
11101	38	-0.2663871418588357
11101	39	0.30964360587002204
11101	40	-0.19559748427673185
11101	41	0.429769392033549
11101	42	0.24220824598182844
11101	43	0.12271139063592074
11101	44	0.4533892382948963
11101	45	-0.046121593291402974
11101	46	0.2401118099231283
11101	47	-0.03480083857442651
11101	48	0.5774283717680007
11101	49	0.3432564640111764
11101	50	0.6265548567435388
11102	1	0.055765199161424306
11102	2	-0.08965758211041219
11102	3	-0.15471698113207566
11102	4	0.12389937106918225
11102	5	0.07875611460517007
11102	6	-0.1313067784765849
11102	7	0.06212438853948754
11102	8	0.03424178895877361
11102	9	-0.3635220125786205
11102	10	0.15883997204751665
11102	11	-0.32851153039832326
11102	12	-0.07931516422082296
11102	13	-0.13508036338225082
11102	14	-0.11201956673654223
11102	15	-0.4353598881900709
11102	16	-0.6844164919636597
11102	17	0.11313766596785513
11102	18	-0.08106219426974093
11102	19	-0.6082459818308905
11102	20	-0.2621243885394833
11102	21	-0.07064989517819953
11102	22	0.009783368273936333
11102	23	-0.10083857442348432
11102	24	-0.322571628232005
11102	25	-0.31698113207546896
11102	26	0.10034940600978359
11102	27	-0.04779874213836166
11102	28	0.18238993710691886
11102	29	-0.5090845562543649
11102	30	-0.17973445143256583
11102	31	-0.40475192173305174
11102	32	-0.5742138364779876
11102	33	-0.1126484975541544
11102	34	0.17784765897973642
11102	35	-0.16079664570230534
11102	36	-0.3429769392033535
11102	37	-0.05904961565339306
11102	38	0.2663871418588428
11102	39	-0.30964360587001494
11102	40	0.19559748427673185
11102	41	-0.4297693920335419
11102	42	-0.24220824598182844
11102	43	-0.12271139063591363
11102	44	-0.4533892382948963
11102	45	0.04612159329141008
11102	46	-0.2401118099231283
11102	47	0.0348008385744194
11102	48	-0.5774283717679936
11102	49	-0.3432564640111835
11102	50	-0.6265548567435388
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	1.0
25	7.0
26	10.0
27	33.0
28	52.0
29	78.0
30	99.0
31	117.0
32	187.0
33	293.0
34	604.0
35	2516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.5	23.125	23.625	26.75
2	20.5	19.025	26.950000000000003	33.525
3	23.325000000000003	25.924999999999997	24.3	26.450000000000003
4	22.85	22.175	26.950000000000003	28.025
5	15.6	28.549999999999997	26.75	29.099999999999998
6	29.939969984992498	24.73736868434217	23.261630815407706	22.061030515257627
7	26.174999999999997	19.575	28.725	25.525
8	22.075	20.45	23.125	34.35
9	25.224999999999998	17.849999999999998	24.349999999999998	32.574999999999996
10	26.174999999999997	20.150000000000002	22.325	31.35
11	27.125	18.75	20.200000000000003	33.925
12	28.549999999999997	18.475	23.7	29.275000000000002
13	28.175	24.6	24.725	22.5
14	22.925	20.375	26.35	30.349999999999998
15	21.275	23.75	23.625	31.35
16	20.875	22.900000000000002	29.375	26.85
17	18.575	20.724999999999998	26.575	34.125
18	26.6	16.400000000000002	34.9	22.1
19	24.4	20.549999999999997	29.825000000000003	25.224999999999998
20	24.3	15.575	25.45	34.675
21	23.3	20.45	23.925	32.324999999999996
22	18.625	21.075	31.6	28.7
23	26.200000000000003	14.575	22.375	36.85
24	25.95	12.75	23.325000000000003	37.974999999999994
25	2.35	8.025	74.15	15.475
26	18.25	19.625	22.425	39.7
27	26.450000000000003	24.05	23.849999999999998	25.650000000000002
28	17.549999999999997	20.025000000000002	30.599999999999998	31.825
29	31.8	21.825	17.2	29.175
30	26.825	20.05	24.05	29.075
31	23.025000000000002	25.2	22.75	29.025000000000002
32	22.75	20.7	23.549999999999997	33.0
33	32.4	19.900000000000002	19.45	28.249999999999996
34	25.674999999999997	19.950000000000003	31.55	22.825
35	25.724999999999998	19.55	23.7	31.025000000000002
36	24.75	13.900000000000002	24.2	37.15
37	19.775000000000002	25.525	27.925	26.775
38	30.599999999999998	11.35	24.05	34.0
39	27.35	18.075	24.2	30.375000000000004
40	23.375	25.025	26.650000000000002	24.95
41	24.2	21.775	20.1	33.925
42	25.275	23.575	21.475	29.675
43	26.35	25.374999999999996	25.474999999999998	22.8
44	26.474999999999998	19.225	23.549999999999997	30.75
45	18.35	22.275	23.200000000000003	36.175000000000004
46	22.825	22.075	31.674999999999997	23.425
47	27.250000000000004	17.025000000000002	22.15	33.575
48	23.825	25.324999999999996	22.075	28.775000000000002
49	22.625	16.975	34.300000000000004	26.1
50	19.900000000000002	13.975000000000001	21.0	45.125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	0.5
28	0.0
29	0.0
30	0.0
31	0.5
32	1.0
33	0.5
34	0.0
35	1.5
36	3.0
37	15.5
38	28.0
39	29.0
40	30.0
41	80.0
42	130.0
43	103.5
44	77.0
45	166.0
46	255.0
47	277.5
48	300.0
49	436.0
50	572.0
51	556.0
52	540.0
53	433.5
54	327.0
55	453.5
56	580.0
57	526.5
58	473.0
59	366.0
60	259.0
61	208.0
62	157.0
63	132.0
64	107.0
65	80.5
66	54.0
67	39.5
68	25.0
69	30.0
70	35.0
71	37.0
72	39.0
73	21.0
74	3.0
75	1.5
76	0.0
77	2.0
78	4.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69093231162196	28.849999999999998
2	10.472541507024266	8.200000000000001
3	4.21455938697318	4.95
4	2.937420178799489	4.6
5	1.6602809706257982	3.25
6	1.0217113665389528	2.4
7	1.0855683269476373	2.9749999999999996
8	0.70242656449553	2.1999999999999997
9	0.5108556832694764	1.7999999999999998
>10	3.1289910600255424	24.925
>50	0.5747126436781609	15.85
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	97	2.4250000000000003	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	81	2.025	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	80	2.0	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	70	1.7500000000000002	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	69	1.725	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	69	1.725	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	56	1.4000000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	56	1.4000000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	56	1.4000000000000001	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	42	1.05	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	40	1.0	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	38	0.95	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	38	0.95	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	37	0.9249999999999999	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	37	0.9249999999999999	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	36	0.8999999999999999	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	33	0.8250000000000001	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	29	0.7250000000000001	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	28	0.7000000000000001	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	27	0.675	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	26	0.65	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	26	0.65	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	25	0.625	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	25	0.625	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	24	0.6	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	23	0.575	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCAT	23	0.575	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	22	0.5499999999999999	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	21	0.525	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	20	0.5	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	20	0.5	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	20	0.5	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	19	0.475	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	19	0.475	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	16	0.4	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	16	0.4	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	15	0.375	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	15	0.375	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	14	0.35000000000000003	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	14	0.35000000000000003	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	14	0.35000000000000003	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	13	0.325	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	13	0.325	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	13	0.325	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	13	0.325	No Hit
GCAGGGTGACCAGCTTTCCTTCCTTGATGGCAGCATGGAAGCTGTGGGCG	13	0.325	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	12	0.3	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	12	0.3	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	12	0.3	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	12	0.3	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	11	0.27499999999999997	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	11	0.27499999999999997	No Hit
AGCACCTTCTTCACATTCACCACGCCGTAGAGTGCTGCCGTCACGTGCTG	10	0.25	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	10	0.25	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	10	0.25	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	10	0.25	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	10	0.25	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	10	0.25	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	9	0.22499999999999998	No Hit
CGGCGCCAATTCCTTTCACCGAGTTCGCGAGGCAAGACGCGGAGACTCGC	9	0.22499999999999998	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	9	0.22499999999999998	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	9	0.22499999999999998	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	9	0.22499999999999998	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	9	0.22499999999999998	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	9	0.22499999999999998	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	9	0.22499999999999998	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	8	0.2	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTT	8	0.2	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	8	0.2	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	8	0.2	No Hit
TTTGGAGCCGAGACTGTTGTTCCATAATGTGGTTGTTCAGAATCAGCGCC	8	0.2	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	8	0.2	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	8	0.2	No Hit
TCCTCCAAGGCCAGCTCCTTCAGCTCCCTCTGCAGCTGCTCACTGTCATC	8	0.2	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	8	0.2	No Hit
GCACGATGTTCGCACTCCTGTTCGTCCCAAGGCAGCCAGCTCATCAGTCA	8	0.2	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	7	0.17500000000000002	No Hit
ACACCGCTTTCATCGCCATGCTTCCCCGGGAGACGCGCGGCGGCCTCCTC	7	0.17500000000000002	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	7	0.17500000000000002	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	7	0.17500000000000002	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
TTGGCGCGGCACGTGTAGCTCAGGTTCCTCCGCACGCTGCGCTTGAAGAA	7	0.17500000000000002	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	7	0.17500000000000002	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	7	0.17500000000000002	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	7	0.17500000000000002	No Hit
CAACATTCTGTCCCAGCGACCACGCCAGCCCAGCTTCTTTATCTTTTCCA	7	0.17500000000000002	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	7	0.17500000000000002	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	7	0.17500000000000002	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
GTGGAGACTCCTCACAATGCACCGCCTCGAATTGCTCTTGCCCTTCCTAC	7	0.17500000000000002	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	7	0.17500000000000002	No Hit
ATAGACATCACCCTGGTAGGTCGCTGGTGTGCCCTCCAGTGTGGTCCGTA	7	0.17500000000000002	No Hit
GCAGACGCAACTCTCGGTAGGTCCTTTTGGCGTGAATGATGGACTGAAAC	6	0.15	No Hit
AGGTCAGCACCTGCTGGATCCAGTTGATCACACGTGTGACACGGGTGTAG	6	0.15	No Hit
AATCATCTTCCTGTGACCATTTCCAATCCTCCAGAGGCAGTCTCCAGTCT	6	0.15	No Hit
ATGGCCATGATGGCATTGCGGATGTCCTCCTCATCAGCGTTGGAGCTTAC	6	0.15	No Hit
TGGTGCATACGCTCCTGTGCGATGTCCCGCGGCAATCGGTCCATGTGATC	6	0.15	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	6	0.15	No Hit
CTGGACTCGGAGTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCT	6	0.15	No Hit
CCTGGCTCAGTGCAGTTCCGCAAATGTAAGTCCTCCTTGGTGAAGCACAG	6	0.15	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	6	0.15	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	6	0.15	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	6	0.15	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	6	0.15	No Hit
CAGCTGCTGCCCGCCGACATGTCTCCTGCACGCCGCCCACGACCCTCCAG	6	0.15	No Hit
TGGAAGGACACTGGTCAGCAATCCTCAACACAGCACGCCACTTCCCAAAG	6	0.15	No Hit
CAGGTAGAAGTGGCGCTGAGTTTCCCCGAGATCTCCTCCCGCTGTTCCTC	6	0.15	No Hit
GGTAGGTTACCCTGTTGACATCAGCCCCACATTTCAACAAGAGCGAAACC	6	0.15	No Hit
TCAAGGTGTAGAGTGAAGTCCTCCTTAGCTGTCCGGATATTCAAGGATGC	5	0.125	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	5	0.125	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	5	0.125	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	5	0.125	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	5	0.125	No Hit
CTTTAGTCCAGGCCACTTCAGTCATCAGCAAGCGGAGGTAGTACATGGCA	5	0.125	No Hit
CGATGACCTTGGTTTGCTCGAACACGACCTTCTCGGTGATGCCGGCTTCC	5	0.125	No Hit
CTCAAGAGGTATGTTCCTATGAGCTAGCCTGAGCTTCCTTGCAATGCGCT	5	0.125	No Hit
CTGCCCGAGATGCTCATGTGCTCCTGCTCACTCAACATCTCTGGCCGTTC	5	0.125	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	5	0.125	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	5	0.125	No Hit
GCATGGCTCCATTCTTCACTCCAGTACTGTCTGCCATCAGTGCCGAGACC	5	0.125	No Hit
GTCAACCTCATGTGGCAGGTTCCCAATGAAGAGCTGGTGACTGTCAGGGT	5	0.125	No Hit
CTGGGTCGGATCTTGTGTTCATCCTGTTGCTCTCCCCTGGCGTCTGCACA	5	0.125	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	5	0.125	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	5	0.125	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	5	0.125	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	5	0.125	No Hit
CACTGGTTTGCCTGATTAAGCTGTTCACACCCAGAACGGTAGCAGCTGCA	5	0.125	No Hit
GGGACAGCAAAGCTCTATATCTCTTCCATTCCTCATCCTTAGATATTGAG	5	0.125	No Hit
GTGGCTCATTCCAGAAGCTGAACTCCAGAAGCAACTGGTTTCTCCTGGAC	5	0.125	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	5	0.125	No Hit
ATTTCGTCTTCTGAGATGGAGTCCTTGCGACTTGTGCTTCGAAGGCGAAC	5	0.125	No Hit
TACTGTCATGGAGACCAGGTTCTCCCCTCGAAGCAACACCAGACCAAGGA	5	0.125	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	5	0.125	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
Read 274591 spots for SRR25943834.sra
Written 274591 spots for SRR25943834.sra
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
Read 274588 spots for SRR25943834.sra
Written 274588 spots for SRR25943834.sra
SRR ids: ['SRR25943834.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_r7vfwxh1
SRR25943834.sra spots: 2196707
blocks: [[1, 274588], [274589, 549176], [549177, 823764], [823765, 1098352], [1098353, 1372940], [1372941, 1647528], [1647529, 1922116], [1922117, 2196707]]
SRR25943834 file size 379040
SRR25943834 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943834 SRR25943834_1.fastq
Input file:	SRR25943834_1.fastq
trimmed:	SRR25943834-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:38:50 2026 >> started

Tue May  5 05:38:51 2026 >> done (0.659s)
2196707 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
2196707 (100.00%) reads available; of these:
     34 ( 0.00%) trimmed reads available after processing
2196673 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 44	      1	  0.00%
 45	      0	  0.00%
 46	      6	  0.00%
 47	      2	  0.00%
 48	      7	  0.00%
 49	     18	  0.00%
 50	2196673	100.00%
2196707 reads passed initial QC


criterion=sequence-density
sequence-density=5.84
sequence-density-rank=1
fanout-score=11.98
fanout-score-rank=1
prefix-density=5.98
prefix-fanout=11.7
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC


criterion=fanout-score
sequence-density=5.84
sequence-density-rank=1
fanout-score=11.98
fanout-score-rank=1
prefix-density=5.98
prefix-fanout=11.7
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC -o SRR25943834 -
Input file:	STDIN
trimmed:	SRR25943834-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Tue May  5 05:38:54 2026 >> started

Tue May  5 05:38:55 2026 >> done (0.607s)
1464471 reads processed; of these:
  56403 ( 3.85%) short reads filtered out after trimming by size control
   3767 ( 0.26%) empty reads filtered out after trimming by size control
1404301 (95.89%) reads available; of these:
  79072 ( 5.63%) trimmed reads available after processing
1325229 (94.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    230	  0.02%
 19	    304	  0.02%
 20	    357	  0.03%
 21	    339	  0.02%
 22	    512	  0.04%
 23	    612	  0.04%
 24	   1357	  0.10%
 25	   2135	  0.15%
 26	   1730	  0.12%
 27	   1812	  0.13%
 28	   1822	  0.13%
 29	   1753	  0.12%
 30	   1816	  0.13%
 31	   2484	  0.18%
 32	   1760	  0.13%
 33	   1675	  0.12%
 34	   1768	  0.13%
 35	   1616	  0.12%
 36	   1762	  0.13%
 37	   1500	  0.11%
 38	   1855	  0.13%
 39	   2329	  0.17%
 40	   1489	  0.11%
 41	   1479	  0.11%
 42	   2255	  0.16%
 43	   1631	  0.12%
 44	   2042	  0.15%
 45	   2860	  0.20%
 46	  16821	  1.20%
 47	  18970	  1.35%
 48	      4	  0.00%
 49	      8	  0.00%
 50	1325214	 94.37%


criterion=sequence-density
sequence-density=3.09
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=18
prefix-density=0.00
prefix-fanout=1.0
sequence=CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATGCC


criterion=fanout-score
sequence-density=1.76
sequence-density-rank=6
fanout-score=12.07
fanout-score-rank=1
prefix-density=1.86
prefix-fanout=11.4
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
                                 Started job on |	May 05 05:39:00
                             Started mapping on |	May 05 05:39:00
                                    Finished on |	May 05 05:39:05
       Mapping speed, Million of reads per hour |	1538.31

                          Number of input reads |	2136537
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1815877
                        Uniquely mapped reads % |	84.99%
                          Average mapped length |	49.26
                       Number of splices: Total |	2343
            Number of splices: Annotated (sjdb) |	1540
                       Number of splices: GT/AG |	1953
                       Number of splices: GC/AG |	70
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	320
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.10%
                        Deletion average length |	1.25
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.06
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	163123
             % of reads mapped to multiple loci |	7.63%
        Number of reads mapped to too many loci |	52585
             % of reads mapped to too many loci |	2.46%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.90%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	157537	157537	157537
N_multimapping	163123	163123	163123
N_noFeature	2242	1733605	2376
N_ambiguous	95334	30	14081
UnstrandedReadsAssigned:1718301 PositiveStrandReadsAssigned:82242 NegativeStrandReadsAssigned:1799420
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943834 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943834-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,136,537 reads, 1,881,960 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,094 rounds

 109283 SRR25943834.ke.tsv
  52636 SRR25943834.se.tsv
 161919 total
==> SRR25943834.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943834.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943834 completed mapping pipeline successfully
