Starting /dee2/code/volunteer_pipeline.sh SRR25943858
    current disk space = 489233332224
    free memory = 390321600 
SRR25943858 SRAfilesize
8e8a21e6bb4ba9d72bd1814d3caf3f17  SRR25943858.sra
SRR25943858.sra file validated
SRR25943858 is single end
SRR25943858 is conventional basespace
SRR25943858 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943858_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3925	32.0	32.0	32.0	32.0	32.0
2	31.52375	32.0	32.0	32.0	32.0	32.0
3	31.56575	32.0	32.0	32.0	32.0	32.0
4	31.62025	32.0	32.0	32.0	32.0	32.0
5	31.6415	32.0	32.0	32.0	32.0	32.0
6	35.0385	36.0	36.0	36.0	36.0	36.0
7	35.21025	36.0	36.0	36.0	36.0	36.0
8	34.9065	36.0	36.0	36.0	32.0	36.0
9	35.00475	36.0	36.0	36.0	32.0	36.0
10	34.9915	36.0	36.0	36.0	32.0	36.0
11	35.06225	36.0	36.0	36.0	36.0	36.0
12	35.12375	36.0	36.0	36.0	36.0	36.0
13	35.1135	36.0	36.0	36.0	36.0	36.0
14	35.16525	36.0	36.0	36.0	36.0	36.0
15	35.0045	36.0	36.0	36.0	36.0	36.0
16	35.07175	36.0	36.0	36.0	36.0	36.0
17	35.0225	36.0	36.0	36.0	36.0	36.0
18	34.93525	36.0	36.0	36.0	32.0	36.0
19	35.08625	36.0	36.0	36.0	36.0	36.0
20	35.05825	36.0	36.0	36.0	36.0	36.0
21	34.99525	36.0	36.0	36.0	36.0	36.0
22	34.9755	36.0	36.0	36.0	36.0	36.0
23	35.05975	36.0	36.0	36.0	36.0	36.0
24	35.05225	36.0	36.0	36.0	36.0	36.0
25	35.185	36.0	36.0	36.0	36.0	36.0
26	34.8805	36.0	36.0	36.0	36.0	36.0
27	34.8795	36.0	36.0	36.0	36.0	36.0
28	34.78	36.0	36.0	36.0	32.0	36.0
29	34.9235	36.0	36.0	36.0	36.0	36.0
30	34.89775	36.0	36.0	36.0	36.0	36.0
31	35.001	36.0	36.0	36.0	36.0	36.0
32	35.04775	36.0	36.0	36.0	36.0	36.0
33	34.8775	36.0	36.0	36.0	32.0	36.0
34	34.94725	36.0	36.0	36.0	32.0	36.0
35	34.935	36.0	36.0	36.0	36.0	36.0
36	34.9915	36.0	36.0	36.0	36.0	36.0
37	34.8475	36.0	36.0	36.0	32.0	36.0
38	34.7545	36.0	36.0	36.0	32.0	36.0
39	34.9225	36.0	36.0	36.0	32.0	36.0
40	34.519	36.0	36.0	36.0	32.0	36.0
41	34.791	36.0	36.0	36.0	32.0	36.0
42	34.76775	36.0	36.0	36.0	32.0	36.0
43	34.59525	36.0	36.0	36.0	32.0	36.0
44	34.6665	36.0	36.0	36.0	32.0	36.0
45	34.41975	36.0	36.0	36.0	32.0	36.0
46	34.2705	36.0	36.0	36.0	32.0	36.0
47	34.1	36.0	36.0	36.0	32.0	36.0
48	34.0585	36.0	36.0	36.0	32.0	36.0
49	34.26025	36.0	36.0	36.0	32.0	36.0
50	33.917	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	-0.136360202462793
11101	2	-0.0789579713429518
11101	3	0.37820754954546487
11101	4	0.02482939235979842
11101	5	0.11848102540857752
11101	6	-0.0034876986225498285
11101	7	0.16954999874090504
11101	8	0.06922515172118437
11101	9	0.2823399058195406
11101	10	0.11640351539875837
11101	11	0.39049633602780176
11101	12	-0.11218554053033358
11101	13	0.2602805268061772
11101	14	-0.221273702500568
11101	15	0.39286343834201887
11101	16	-0.21242225076175458
11101	17	0.07573468308529385
11101	18	0.011256326962296725
11101	19	-0.04185238347056952
11101	20	0.042683387474504286
11101	21	0.055866132809555324
11101	22	0.04220493062375397
11101	23	0.2848329178313307
11101	24	-0.35118732844804157
11101	25	-0.11577396691093611
11101	26	-0.012691697514540579
11101	27	-0.051245246908912634
11101	28	-0.04975951247764954
11101	29	0.1820654226788534
11101	30	0.34746040140011303
11101	31	-0.02884591171211781
11101	32	0.13196595401777955
11101	33	0.20080078567651327
11101	34	0.10847120445216518
11101	35	0.057540731787156574
11101	36	-0.2771650172496294
11101	37	0.2706806678250402
11101	38	0.210873561481705
11101	39	0.3212460023671042
11101	40	-0.05347384855581083
11101	41	0.04006446576515543
11101	42	-0.18861272695222908
11101	43	0.3674422704036644
11101	44	-0.24867165269068892
11101	45	0.025786306061299058
11101	46	0.45975926065825945
11101	47	0.31241973256779687
11101	48	0.3002064919040066
11101	49	0.26942157084938856
11101	50	0.10898743421218882
11102	1	0.136360202462793
11102	2	0.0789579713429518
11102	3	-0.37820754954546487
11102	4	-0.02482939235980197
11102	5	-0.11848102540857397
11102	6	0.003487698622542723
11102	7	-0.16954999874090504
11102	8	-0.06922515172118437
11102	9	-0.2823399058195477
11102	10	-0.11640351539875837
11102	11	-0.39049633602780176
11102	12	0.11218554053032648
11102	13	-0.2602805268061701
11102	14	0.221273702500568
11102	15	-0.39286343834201887
11102	16	0.21242225076175458
11102	17	-0.07573468308528675
11102	18	-0.011256326962303831
11102	19	0.041852383470576626
11102	20	-0.04268338747449718
11102	21	-0.05586613280954822
11102	22	-0.04220493062376107
11102	23	-0.2848329178313307
11102	24	0.35118732844803446
11102	25	0.115773966910929
11102	26	0.012691697514547684
11102	27	0.05124524690891974
11102	28	0.04975951247765664
11102	29	-0.1820654226788605
11102	30	-0.34746040140011303
11102	31	0.028845911712124916
11102	32	-0.13196595401777955
11102	33	-0.20080078567651327
11102	34	-0.10847120445216518
11102	35	-0.05754073178716368
11102	36	0.2771650172496294
11102	37	-0.2706806678250331
11102	38	-0.210873561481705
11102	39	-0.3212460023671042
11102	40	0.05347384855581794
11102	41	-0.04006446576515543
11102	42	0.18861272695222908
11102	43	-0.3674422704036644
11102	44	0.24867165269068892
11102	45	-0.025786306061291953
11102	46	-0.45975926065825234
11102	47	-0.312419732567804
11102	48	-0.3002064919040066
11102	49	-0.26942157084938145
11102	50	-0.10898743421218171
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	3.0
25	7.0
26	13.0
27	25.0
28	45.0
29	73.0
30	95.0
31	118.0
32	174.0
33	301.0
34	622.0
35	2521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.525	19.55	23.9	29.025000000000002
2	22.475	17.724999999999998	28.175	31.624999999999996
3	27.025	24.775	23.974999999999998	24.224999999999998
4	26.150000000000002	20.0	25.825	28.025
5	16.075	28.15	26.224999999999998	29.549999999999997
6	28.08904452226113	24.96248124062031	24.637318659329665	22.311155577788895
7	29.925	18.675	29.049999999999997	22.35
8	22.7	19.575	21.55	36.175000000000004
9	23.95	18.375	27.250000000000004	30.425
10	27.375	18.775	25.05	28.799999999999997
11	26.200000000000003	25.6	19.75	28.449999999999996
12	31.474999999999998	18.725	22.125	27.675
13	31.4	27.025	20.8	20.775
14	21.925	20.5	26.825	30.75
15	19.375	29.099999999999998	23.275000000000002	28.249999999999996
16	20.549999999999997	26.1	27.85	25.5
17	20.575	19.1	26.8	33.525
18	23.5	16.2	37.35	22.95
19	25.1	21.15	30.425	23.325000000000003
20	26.375	15.425	24.75	33.45
21	21.275	23.775	25.7	29.25
22	22.05	18.25	31.324999999999996	28.375
23	26.85	15.8	25.074999999999996	32.275
24	26.174999999999997	13.55	24.9	35.375
25	3.0	7.7	76.5	12.8
26	19.2	18.375	21.125	41.3
27	27.575	24.925	22.6	24.9
28	18.25	21.625	31.175000000000004	28.95
29	34.699999999999996	22.425	17.325	25.55
30	29.049999999999997	20.225	23.425	27.3
31	26.924999999999997	24.2	18.35	30.525000000000002
32	23.825	19.400000000000002	22.175	34.599999999999994
33	31.25	23.275000000000002	17.549999999999997	27.925
34	26.650000000000002	18.8	32.175	22.375
35	27.425	19.25	24.8	28.525
36	28.1	14.975	23.9	33.025
37	22.0	27.625	26.25	24.125
38	31.225	11.575000000000001	23.549999999999997	33.650000000000006
39	28.849999999999998	20.7	21.475	28.975
40	22.325	23.974999999999998	25.95	27.750000000000004
41	23.674999999999997	23.849999999999998	20.1	32.375
42	27.925	22.125	25.1	24.85
43	25.724999999999998	23.674999999999997	27.275	23.325000000000003
44	27.474999999999998	18.8	24.125	29.599999999999998
45	15.525	25.424999999999997	21.224999999999998	37.824999999999996
46	21.775	24.45	31.7	22.075
47	27.025	14.674999999999999	21.975	36.325
48	22.6	23.7	24.7	28.999999999999996
49	25.7	18.775	30.85	24.675
50	18.05	16.8	22.675	42.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	2.0
29	2.5
30	3.0
31	2.0
32	1.0
33	1.0
34	1.0
35	3.0
36	5.0
37	15.0
38	25.0
39	30.0
40	35.0
41	73.0
42	111.0
43	116.0
44	121.0
45	196.5
46	272.0
47	282.5
48	293.0
49	454.0
50	615.0
51	588.0
52	561.0
53	433.0
54	305.0
55	439.0
56	573.0
57	472.0
58	371.0
59	346.0
60	321.0
61	247.5
62	174.0
63	139.5
64	105.0
65	71.0
66	37.0
67	30.5
68	24.0
69	22.0
70	20.0
71	19.5
72	19.0
73	10.5
74	2.0
75	1.0
76	0.0
77	2.0
78	4.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.05
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03278688524591	28.225
2	10.295081967213115	7.85
3	4.4590163934426235	5.1
4	2.819672131147541	4.3
5	1.4426229508196722	2.75
6	1.3114754098360655	3.0
7	0.9836065573770493	2.625
8	0.5245901639344263	1.6
9	0.39344262295081966	1.35
>10	3.2131147540983602	26.150000000000002
>50	0.45901639344262296	12.65
>100	0.06557377049180328	4.3999999999999995
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	176	4.3999999999999995	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	92	2.3	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	83	2.075	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	75	1.875	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	72	1.7999999999999998	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	71	1.775	No Hit
CTTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	58	1.4500000000000002	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	55	1.375	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	50	1.25	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	44	1.0999999999999999	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	43	1.075	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	43	1.075	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	43	1.075	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	42	1.05	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	39	0.975	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	34	0.8500000000000001	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	32	0.8	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	32	0.8	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCAT	27	0.675	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	24	0.6	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	24	0.6	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	24	0.6	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	24	0.6	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	24	0.6	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	23	0.575	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	22	0.5499999999999999	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	21	0.525	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	20	0.5	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	20	0.5	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	19	0.475	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	19	0.475	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	18	0.44999999999999996	No Hit
ACTCAAATGACTATGACCCTGGTCTTGAAGGCCATAGGCAATATCTTCCC	18	0.44999999999999996	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	17	0.42500000000000004	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	16	0.4	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	16	0.4	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	16	0.4	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	16	0.4	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	16	0.4	No Hit
TGGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	15	0.375	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	14	0.35000000000000003	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	14	0.35000000000000003	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	14	0.35000000000000003	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	14	0.35000000000000003	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	14	0.35000000000000003	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	13	0.325	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	13	0.325	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	12	0.3	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	12	0.3	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	12	0.3	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	11	0.27499999999999997	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	11	0.27499999999999997	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	11	0.27499999999999997	No Hit
GTACCGCTCCGCCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTT	10	0.25	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	10	0.25	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	10	0.25	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	10	0.25	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
GAGCCACCTTATCTCCGCGACGGATCTGGTACTCTTGTCCGCTGGCCATC	9	0.22499999999999998	No Hit
CTGCCATGCTCGGACTTAGCCCACTCCACGGCAGTGACCACGGCACCAGA	9	0.22499999999999998	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	9	0.22499999999999998	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	9	0.22499999999999998	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	9	0.22499999999999998	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	8	0.2	No Hit
GACATAGGTGCTTCCTCCACCAGTTCCTCCATCCTCAGCTAGAAAGTCCG	8	0.2	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	8	0.2	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	8	0.2	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	8	0.2	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
TCTCTGGTCCAGCTGTGGTGGAGCTGGTGAAGGTGAGCAAGCAGAGCGCC	8	0.2	No Hit
CTGCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTC	8	0.2	No Hit
CTTGCGGATGCCATCTTCTTCCACTTCGTAGTAGTCAGGCCCACAGGCTC	7	0.17500000000000002	No Hit
CCTGCTGGTGGTCTGGTCCTGTTCCTGCCCACCTGTGGTCGTAGCATCAG	7	0.17500000000000002	No Hit
ATTCCAATCCACTGGCCTGTCTGCTGGAGGTATCGATGTCCTCAAACAGG	7	0.17500000000000002	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	7	0.17500000000000002	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	7	0.17500000000000002	No Hit
CACTGGTTTGCCTGATTAAGCTGTTCACACCCAGAACGGTAGCAGCTGCA	7	0.17500000000000002	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	7	0.17500000000000002	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	7	0.17500000000000002	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	7	0.17500000000000002	No Hit
CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTA	7	0.17500000000000002	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	7	0.17500000000000002	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	7	0.17500000000000002	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	7	0.17500000000000002	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	7	0.17500000000000002	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	7	0.17500000000000002	No Hit
CCTGTGTCCTGAGTGCTGGAGGCTTCCTTAGTGAGGCCAAAGTCACTGAC	6	0.15	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	6	0.15	No Hit
CCTGTAGCCATCAACACAGTGGACTGAGCTGTGCTGAGTTTCTCTGCAGG	6	0.15	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	6	0.15	No Hit
TAGTAAGTGTTCCGGCGACCGGAGTCAACCTCCCAGGCTGCTCTCTGGTA	6	0.15	No Hit
ACACCGCTTTCATCGCCATGCTTCCCCGGGAGACGCGCGGCGGCCTCCTC	6	0.15	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	6	0.15	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	6	0.15	No Hit
TCCCCACCAAGGCCCAGGTTCTGCAAGTGGGTTTCCACCGCTGCTTGGGT	6	0.15	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	6	0.15	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	6	0.15	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
AACCCGTCTCTACAGGCCACGCTGTTCGCTGTGACGGCGAAGTAGATTGT	6	0.15	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	6	0.15	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	6	0.15	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	6	0.15	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	6	0.15	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	6	0.15	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	6	0.15	No Hit
CAGGTACAAGGCAAGAGGATTTGCCCCAGCAAGCCAACCATTCTCTGAAC	6	0.15	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	5	0.125	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	5	0.125	No Hit
TCAGCGTTCACTAGGCAGACCATCTGCAACACAATGGTGGCTCCCATACG	5	0.125	No Hit
AATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATT	5	0.125	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	5	0.125	No Hit
TCACTCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATC	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
TCATGCAAGAACTCACACTGGTCCCCTTTCTTGCACAGTCCTCTCAGCCA	5	0.125	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	5	0.125	No Hit
GAACGCCTTTACCAAACGGAGTGTTTCTTCCACACTGCGGCCCACCGGAA	5	0.125	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	5	0.125	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	5	0.125	No Hit
AGAGTTCTGGCAAACTCACAACGTTCATAGACCTTGGCCTGAGCAGTGAC	5	0.125	No Hit
TCATACAGAGCCTCATTGTCAATGGAATAGGTTTCATCTGTGTTCTCTAC	5	0.125	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	5	0.125	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	5	0.125	No Hit
AATCGGACCTCTGCCTCTTTGTCTTCGACGCCGTTGGCCACCATCTTGGC	5	0.125	No Hit
AGAGGCAGCACTTGCTGGAGAAGGTCAGGCGTCCTTGCGTCAGACTGGCT	5	0.125	No Hit
CCAGTACCAGCTACTCCGCTCCTCCGAGTCGAAGTTGCCCGCCATGGCCG	5	0.125	No Hit
CTGTTTCCTGCAGTCCAGGCGAGATTGACAGCAGTCTCCAACTTCTTGTT	5	0.125	No Hit
ATAGACATCACCCTGGTAGGTCGCTGGTGTGCCCTCCAGTGTGGTCCGTA	5	0.125	No Hit
TCTGACACTTTCACTCCCAACGGCTTCACAGCCTCATCGGAGCGCAGGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCATT	20	6.952051E-4	44.000004	19
TGAGAAT	20	6.952051E-4	44.000004	9
AGCCCAT	20	6.952051E-4	44.000004	20
CTACAAA	20	6.952051E-4	44.000004	35
GAGAATC	20	6.952051E-4	44.000004	10
AAACTTC	20	6.952051E-4	44.000004	39
GAGGCAG	20	6.952051E-4	44.000004	36
TTCATTC	20	6.952051E-4	44.000004	20
ACAAACT	20	6.952051E-4	44.000004	37
TCCTTCT	20	6.952051E-4	44.000004	30
CATGAGA	20	6.952051E-4	44.000004	7
GTGTCCA	20	6.952051E-4	44.000004	7
GCCCATA	20	6.952051E-4	44.000004	21
CAGTTCT	20	6.952051E-4	44.000004	12
CTCCTTC	20	6.952051E-4	44.000004	29
CCAGTTC	20	6.952051E-4	44.000004	11
GCGATCT	20	6.952051E-4	44.000004	42
TGTCCAG	20	6.952051E-4	44.000004	8
AATCAGG	20	6.952051E-4	44.000004	13
TCAGGCC	20	6.952051E-4	44.000004	9
>>END_MODULE
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112519 spots for SRR25943858.sra
Written 112519 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
Read 112516 spots for SRR25943858.sra
Written 112516 spots for SRR25943858.sra
SRR ids: ['SRR25943858.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_snz072v8
SRR25943858.sra spots: 1800259
blocks: [[1, 112516], [112517, 225032], [225033, 337548], [337549, 450064], [450065, 562580], [562581, 675096], [675097, 787612], [787613, 900128], [900129, 1012644], [1012645, 1125160], [1125161, 1237676], [1237677, 1350192], [1350193, 1462708], [1462709, 1575224], [1575225, 1687740], [1687741, 1800259]]
SRR25943858 file size 1
SRR25943858 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25943858 SRR25943858_1.fastq
Input file:	SRR25943858_1.fastq
trimmed:	SRR25943858-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Tue May  5 09:51:22 2026 >> started

Tue May  5 09:51:23 2026 >> done (1.073s)
1800259 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
1800259 (100.00%) reads available; of these:
     42 ( 0.00%) trimmed reads available after processing
1800217 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      4	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      9	  0.00%
 47	      4	  0.00%
 48	      9	  0.00%
 49	     15	  0.00%
 50	1800217	100.00%
1800259 reads passed initial QC


criterion=sequence-density
sequence-density=6.59
sequence-density-rank=1
fanout-score=12.56
fanout-score-rank=2
prefix-density=6.75
prefix-fanout=12.3
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC


criterion=fanout-score
sequence-density=0.51
sequence-density-rank=31
fanout-score=14.19
fanout-score-rank=1
prefix-density=7.15
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 16 -x CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC -o SRR25943858 -
Input file:	STDIN
trimmed:	SRR25943858-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACTGCTTATTCATCTCGTATGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	16
Tue May  5 09:51:30 2026 >> started

Tue May  5 09:51:32 2026 >> done (1.602s)
1285899 reads processed; of these:
  55661 ( 4.33%) short reads filtered out after trimming by size control
   3925 ( 0.31%) empty reads filtered out after trimming by size control
1226313 (95.37%) reads available; of these:
  72787 ( 5.94%) trimmed reads available after processing
1153526 (94.06%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    223	  0.02%
 19	    285	  0.02%
 20	    368	  0.03%
 21	    292	  0.02%
 22	    374	  0.03%
 23	    665	  0.05%
 24	   1042	  0.08%
 25	   2260	  0.18%
 26	   1777	  0.14%
 27	   1801	  0.15%
 28	   1871	  0.15%
 29	   1822	  0.15%
 30	   1762	  0.14%
 31	   2379	  0.19%
 32	   1820	  0.15%
 33	   2133	  0.17%
 34	   1729	  0.14%
 35	   1677	  0.14%
 36	   1435	  0.12%
 37	   1506	  0.12%
 38	   2009	  0.16%
 39	   2683	  0.22%
 40	   1555	  0.13%
 41	   1551	  0.13%
 42	   2147	  0.18%
 43	   1809	  0.15%
 44	   2112	  0.17%
 45	   2588	  0.21%
 46	  11261	  0.92%
 47	  17862	  1.46%
 48	      5	  0.00%
 49	      9	  0.00%
 50	1153501	 94.06%


criterion=sequence-density
sequence-density=5.28
sequence-density-rank=1
fanout-score=1.21
fanout-score-rank=8
prefix-density=0.08
prefix-fanout=1.2
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCT


criterion=fanout-score
sequence-density=0.52
sequence-density-rank=31
fanout-score=14.19
fanout-score-rank=1
prefix-density=7.39
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
                                 Started job on |	May 05 09:51:49
                             Started mapping on |	May 05 09:51:50
                                    Finished on |	May 05 09:51:59
       Mapping speed, Million of reads per hour |	696.27

                          Number of input reads |	1740673
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1344256
                        Uniquely mapped reads % |	77.23%
                          Average mapped length |	49.11
                       Number of splices: Total |	1752
            Number of splices: Annotated (sjdb) |	954
                       Number of splices: GT/AG |	1354
                       Number of splices: GC/AG |	78
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	320
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.11%
                        Deletion average length |	1.29
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	176836
             % of reads mapped to multiple loci |	10.16%
        Number of reads mapped to too many loci |	123450
             % of reads mapped to too many loci |	7.09%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.51%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	219581	219581	219581
N_multimapping	176836	176836	176836
N_noFeature	7949	1307283	8100
N_ambiguous	48748	21	12497
UnstrandedReadsAssigned:1287559 PositiveStrandReadsAssigned:36952 NegativeStrandReadsAssigned:1323659
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943858 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943858-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,740,673 reads, 1,526,786 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,113 rounds

 109283 SRR25943858.ke.tsv
  52636 SRR25943858.se.tsv
 161919 total
==> SRR25943858.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943858.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943858 completed mapping pipeline successfully
