Starting /dee2/code/volunteer_pipeline.sh SRR25943851
    current disk space = 489110460416
    free memory = 392901424 
SRR25943851 SRAfilesize
e90af113fbc16218c0235f221b745479  SRR25943851.sra
SRR25943851.sra file validated
SRR25943851 is single end
SRR25943851 is conventional basespace
SRR25943851 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38325	32.0	32.0	32.0	32.0	32.0
2	31.51925	32.0	32.0	32.0	32.0	32.0
3	31.592	32.0	32.0	32.0	32.0	32.0
4	31.597	32.0	32.0	32.0	32.0	32.0
5	31.60125	32.0	32.0	32.0	32.0	32.0
6	35.04525	36.0	36.0	36.0	36.0	36.0
7	34.99575	36.0	36.0	36.0	36.0	36.0
8	34.93825	36.0	36.0	36.0	32.0	36.0
9	35.017	36.0	36.0	36.0	32.0	36.0
10	35.043	36.0	36.0	36.0	36.0	36.0
11	35.043	36.0	36.0	36.0	36.0	36.0
12	35.012	36.0	36.0	36.0	36.0	36.0
13	35.07925	36.0	36.0	36.0	36.0	36.0
14	35.12375	36.0	36.0	36.0	36.0	36.0
15	34.99775	36.0	36.0	36.0	36.0	36.0
16	34.9175	36.0	36.0	36.0	36.0	36.0
17	34.938	36.0	36.0	36.0	32.0	36.0
18	34.89425	36.0	36.0	36.0	32.0	36.0
19	35.016	36.0	36.0	36.0	36.0	36.0
20	34.98675	36.0	36.0	36.0	36.0	36.0
21	34.89425	36.0	36.0	36.0	36.0	36.0
22	34.989	36.0	36.0	36.0	36.0	36.0
23	35.031	36.0	36.0	36.0	36.0	36.0
24	34.94825	36.0	36.0	36.0	36.0	36.0
25	35.0725	36.0	36.0	36.0	36.0	36.0
26	34.813	36.0	36.0	36.0	32.0	36.0
27	34.8825	36.0	36.0	36.0	32.0	36.0
28	34.7775	36.0	36.0	36.0	32.0	36.0
29	34.8645	36.0	36.0	36.0	32.0	36.0
30	34.808	36.0	36.0	36.0	32.0	36.0
31	34.92175	36.0	36.0	36.0	36.0	36.0
32	34.803	36.0	36.0	36.0	32.0	36.0
33	34.675	36.0	36.0	36.0	32.0	36.0
34	34.73475	36.0	36.0	36.0	32.0	36.0
35	34.96125	36.0	36.0	36.0	36.0	36.0
36	34.8845	36.0	36.0	36.0	32.0	36.0
37	34.69425	36.0	36.0	36.0	32.0	36.0
38	34.75825	36.0	36.0	36.0	32.0	36.0
39	34.81175	36.0	36.0	36.0	32.0	36.0
40	34.37425	36.0	36.0	36.0	32.0	36.0
41	34.657	36.0	36.0	36.0	32.0	36.0
42	34.63125	36.0	36.0	36.0	32.0	36.0
43	34.539	36.0	36.0	36.0	32.0	36.0
44	34.618	36.0	36.0	36.0	32.0	36.0
45	34.46	36.0	36.0	36.0	32.0	36.0
46	34.411	36.0	36.0	36.0	32.0	36.0
47	34.46425	36.0	36.0	36.0	32.0	36.0
48	34.342	36.0	36.0	36.0	32.0	36.0
49	34.43525	36.0	36.0	36.0	32.0	36.0
50	34.208	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	7.0
25	3.0
26	18.0
27	20.0
28	43.0
29	66.0
30	103.0
31	139.0
32	192.0
33	281.0
34	674.0
35	2453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.625	20.974999999999998	28.175	28.225
2	22.775000000000002	18.625	24.775	33.825
3	26.5	27.224999999999998	21.3	24.975
4	22.75	23.799999999999997	27.625	25.825
5	17.150000000000002	26.5	26.325	30.025000000000002
6	31.256256256256254	26.101101101101097	22.22222222222222	20.42042042042042
7	25.1	23.575	30.975	20.349999999999998
8	26.3	21.475	18.4	33.825
9	26.8	19.275000000000002	22.225	31.7
10	21.25	20.45	27.575	30.725
11	28.725	22.225	18.9	30.15
12	28.499999999999996	20.724999999999998	24.099999999999998	26.674999999999997
13	26.63165791447862	28.182045511377847	23.23080770192548	21.955488872218055
14	21.0	21.349999999999998	25.224999999999998	32.425
15	20.3	28.000000000000004	21.65	30.049999999999997
16	19.2	25.275	29.925	25.6
17	21.95	19.275000000000002	27.0	31.775
18	23.95	16.45	36.225	23.375
19	24.65	22.2	29.15	24.0
20	28.775000000000002	17.675	23.575	29.975
21	21.425	21.125	27.275	30.175
22	18.025	21.525	32.1	28.349999999999998
23	27.775	14.299999999999999	25.275	32.65
24	28.349999999999998	14.825	20.925	35.9
25	1.075	4.3999999999999995	81.825	12.7
26	17.2	20.65	22.625	39.525
27	28.15	25.924999999999997	18.825	27.1
28	16.425	20.5	32.2	30.875000000000004
29	30.0	24.275	19.5	26.224999999999998
30	26.174999999999997	20.599999999999998	24.4	28.825
31	23.599999999999998	26.200000000000003	23.400000000000002	26.8
32	22.475	22.5	23.45	31.574999999999996
33	31.724999999999998	21.075	19.275000000000002	27.925
34	23.225	20.225	33.275	23.275000000000002
35	24.224999999999998	19.45	25.1	31.225
36	27.800000000000004	17.5	22.775000000000002	31.924999999999997
37	21.825	26.75	25.424999999999997	26.0
38	27.725	12.325	24.8	35.15
39	29.025000000000002	21.7	22.400000000000002	26.875
40	23.325000000000003	23.974999999999998	26.650000000000002	26.05
41	25.025	21.775	19.075	34.125
42	26.594946209657245	23.34250688016012	21.065799349512133	28.996747560670507
43	27.250000000000004	25.7	24.05	23.0
44	26.375	18.625	21.85	33.15
45	19.5847923961981	25.962981490745374	22.061030515257627	32.3911955977989
46	23.7	23.95	28.449999999999996	23.9
47	25.424999999999997	20.3	17.175	37.1
48	23.592694520890667	27.87090317738304	21.591193395046286	26.945208906680012
49	25.70642660665166	20.155038759689923	30.582645661415352	23.55588897224306
50	21.9	16.775000000000002	18.8	42.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.0
31	1.0
32	1.0
33	0.5
34	0.0
35	2.0
36	4.0
37	20.5
38	37.0
39	46.5
40	56.0
41	115.5
42	175.0
43	164.0
44	153.0
45	210.0
46	267.0
47	238.0
48	209.0
49	405.0
50	601.0
51	613.0
52	625.0
53	480.5
54	336.0
55	458.5
56	581.0
57	511.0
58	441.0
59	333.0
60	225.0
61	161.0
62	97.0
63	82.5
64	68.0
65	59.0
66	50.0
67	42.5
68	35.0
69	24.5
70	14.0
71	18.5
72	23.0
73	12.0
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.1
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.025
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.075
43	0.0
44	0.0
45	0.05
46	0.0
47	0.0
48	0.075
49	0.025
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.13167013167013	25.3
2	10.464310464310463	7.55
3	4.92030492030492	5.325
4	3.6729036729036726	5.3
5	2.148302148302148	3.875
6	1.8711018711018712	4.05
7	0.8316008316008316	2.1
8	0.8316008316008316	2.4
9	0.5544005544005544	1.7999999999999998
>10	4.088704088704088	30.075000000000003
>50	0.4158004158004158	9.325
>100	0.0693000693000693	2.9000000000000004
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	116	2.9000000000000004	No Hit
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	99	2.475	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	63	1.575	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	54	1.35	No Hit
TTGTCTAGGCGGCCCTGTTGCTGGTACAGGAAGTAAGCAGTGGTGGCCTG	53	1.325	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	53	1.325	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	51	1.275	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	49	1.225	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	46	1.15	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	45	1.125	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	43	1.075	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	39	0.975	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	38	0.95	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	37	0.9249999999999999	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	37	0.9249999999999999	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	37	0.9249999999999999	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	35	0.8750000000000001	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	33	0.8250000000000001	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	33	0.8250000000000001	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	27	0.675	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	25	0.625	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	24	0.6	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	24	0.6	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	24	0.6	No Hit
TCATCCTGAGACACGGTGATGGTTTTCTTGTCACCCTTGTCATCCAGGAC	24	0.6	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	24	0.6	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	24	0.6	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	23	0.575	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	22	0.5499999999999999	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	22	0.5499999999999999	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	19	0.475	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	19	0.475	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	19	0.475	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	18	0.44999999999999996	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	18	0.44999999999999996	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	18	0.44999999999999996	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	17	0.42500000000000004	No Hit
CTGACCAGGATGGAGGTGACATTGTAGCTATTGTTCTCTTGTAGCTCATA	16	0.4	No Hit
TACTCATCCTTCAGTGCTTCTTTGTCCAGGCAAAATGTGGCAAGGCCCCT	16	0.4	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	15	0.375	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	14	0.35000000000000003	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	14	0.35000000000000003	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	13	0.325	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	13	0.325	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	13	0.325	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	12	0.3	No Hit
TTGAGGTGGTCAGCATCCCTGCACCAAACAGGTCACGCACCACCATGAGC	12	0.3	No Hit
TGCAGCTTTGTCTCCACGCAGCTCTTCTCTCTCAGCCTGTCAGCCAGCAG	12	0.3	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	12	0.3	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	11	0.27499999999999997	No Hit
CACTGGTTTGCCTGATTAAGCTGTTCACACCCAGAACGGTAGCAGCTGCA	11	0.27499999999999997	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	11	0.27499999999999997	No Hit
AGAGTTCTGGCAAACTCACAACGTTCATAGACCTTGGCCTGAGCAGTGAC	11	0.27499999999999997	No Hit
GTTGCTAAGCTTCCAACCAAACTCTCCTGCTCCTCAATTTCCTGGTATTC	11	0.27499999999999997	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	11	0.27499999999999997	No Hit
TCAGTGGTACCTTTCTCCTCCAGGTAGCGCTGCACTCGCTCAATGATGGC	11	0.27499999999999997	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	11	0.27499999999999997	No Hit
TCCGAGAAGAGAGTAGCAGGTCAATAGGATATCCATGCTTGCCTACACGG	10	0.25	No Hit
CAAACCTCATGCCAATGCAGTTCCTGGGTCCAATTCCAAAGGGCATATAT	10	0.25	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	10	0.25	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	10	0.25	No Hit
CCAGGATCTTTCCTGCCTTTCTCCTCTCCCTCCGTGTCTGTCTCGCTCGC	10	0.25	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	10	0.25	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	10	0.25	No Hit
TGATGATGACACTAACAATGGTGATAACAACCATCAGGATGCTGAGGACC	10	0.25	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	10	0.25	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	9	0.22499999999999998	No Hit
ACCTTGCCCTTGTACCAAATGCTTTCATTTCCCTCTGGATCCACATCGTC	9	0.22499999999999998	No Hit
CCTCTGAGGTTGATAACAAAGACTTTCTTTTCTGTACTGCCCCACCCTGA	9	0.22499999999999998	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	9	0.22499999999999998	No Hit
TGATTTCAAGCTTCCCTATGGCCCTCATTCTCACTGGCCCGTCATCGATA	9	0.22499999999999998	No Hit
TGCTCAGAGTCTGTCCCGGACAGCTTCTTGTCTTTGCCTTTGCTTCCCGT	9	0.22499999999999998	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	9	0.22499999999999998	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	9	0.22499999999999998	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	8	0.2	No Hit
CCTTAAAGGTGTTCAGTTTGTGGTTAGCAATGCGCCCAGCCACCACAATC	8	0.2	No Hit
CTGTACATGTCAAACTTGACGTTGTAGCCGGCTGCAAACTCCTTGATTTC	8	0.2	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	8	0.2	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	8	0.2	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	8	0.2	No Hit
CCCTCATGGTTCACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	8	0.2	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	8	0.2	No Hit
GTCCACCAGAGCTTCCTTGACTGCCTCCTGTCCGCACAGCACCACAATTC	8	0.2	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	8	0.2	No Hit
TTTAAAGCCTCTTCTAAGAGTCGCCCATCATACTGAGGGTCCCGTGACCT	8	0.2	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	8	0.2	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	7	0.17500000000000002	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	7	0.17500000000000002	No Hit
TACTTAGTGACAGCCTTGGTGCCCTCCGACACGGCGTGCTTGGCCAGCTC	7	0.17500000000000002	No Hit
TCTTCATCCTTCTCTTCAGCCAGCAGGTTGGCAGGCTCCATCACCTCTCC	7	0.17500000000000002	No Hit
GGCTCCAGCTCTGCATCCTGCAACTCTTTGGTGCTCAGAGCAGATGGCTC	7	0.17500000000000002	No Hit
GCGCTTCTCGAACGGATGCTCCTCTTTGTACACGAACTTCATTCTCCCGA	7	0.17500000000000002	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	7	0.17500000000000002	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	7	0.17500000000000002	No Hit
GCAATCAGCAGAGTCAACACCAAGTCACCCAGAACAATCCCAGCCAGTAC	7	0.17500000000000002	No Hit
GCTTCGAACCAGGCACCAGGCATATTCAAATTGCGTGCTCTTGGACACAG	7	0.17500000000000002	No Hit
GCTCTTACATCACTGCTCTGTGTCTTCTCCAATATCCAGTCCATGTACTC	7	0.17500000000000002	No Hit
CCCAAGTAGGTGATGCCAGAATCCTCGTAGAAGCTAGCACTGGTGTTGAC	7	0.17500000000000002	No Hit
AAGCCACCACAGCTCCAAGGATGATCACAGCTCCAAGGACAACCAGAACA	6	0.15	No Hit
TCATGCTTGATGCCCAGAGACTCCCCCTTGTCTGTGCTCAGCGTGCCTGC	6	0.15	No Hit
TCTGTTCCTCAGGCACCTGGACTTTCCTTGCCTTCTTCATGATTGGTTGA	6	0.15	No Hit
TGTAGATGCGGAAGATGTCATCTTTCACATAGGACATAGCCATTGTCAGC	6	0.15	No Hit
ACAACAGCCTTGATCACTTTCTCCTTCAGAGCATCCCTCATCTCATCAAG	6	0.15	No Hit
TGCACTTCTTGAAGAACTCATCTGTTTCCTTGTCTACCACCAGCAGCTTG	6	0.15	No Hit
TGATCTTGCTCTTGCTGCGTTCGATGGTCACGACTCCTCCGCCGAGGTTG	6	0.15	No Hit
TCAGACAGCTTATAGATGCTCTGCCCCTCCTCGCCCACCAGGCGCATGAT	6	0.15	No Hit
TCACAGTGGTACACCTTTGCCCAGCTGTTCCCACGGCGGCAAACAGAACT	6	0.15	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	6	0.15	No Hit
GTCGCCCCGGGAGGTGACGCCGAATCGTCCTCGGTCGCGCGTGGGTAGCT	6	0.15	No Hit
CCAGTCGCATGTTCTCAGCATGGATCATGTCATTGGCAGTCTTCTTGGAC	6	0.15	No Hit
GGATCAGCAATGAACCATCCAAACTGCTCTATGAGAACTGTGGACACAAT	6	0.15	No Hit
AGATTGTCTTGAAGGCATGGATGATCCCTGAATAGGTGTGCTCTCCTTTC	6	0.15	No Hit
TCACTTCGCACTTCTCAAACAATGTAAAATTGGGCTCGAACCCGCCACCA	6	0.15	No Hit
CTCAAGAGGTATGTTCCTATGAGCTAGCCTGAGCTTCCTTGCAATGCGCT	6	0.15	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	6	0.15	No Hit
GGTAGGTTACCCTGTTGACATCAGCCCCACATTTCAACAAGAGCGAAACC	6	0.15	No Hit
AGCATTTCTCGGCTCTCCCTGTGGTCGTTACAGAACATGGCTCTGAAGTA	6	0.15	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTGCTGAGCATCCCATCC	6	0.15	No Hit
AGCAAACAACCCAATCTTGATGACTTGCCACAAAAGTCCCAGGACCAGGT	6	0.15	No Hit
TCAATCAATGTCCATCTCAGGAAGCTTCTTGTCGCCATCAGAAGGCACAG	6	0.15	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	6	0.15	No Hit
GTAGCCCATTGTAGAGTTGTCTCGCAGTGCCTGGGCCTTCATGATCCGTT	6	0.15	No Hit
TTCCACACCATGTTCAGCAGTTCATTGGAATTCACATTGTGCACCACAGC	6	0.15	No Hit
CTTGGAGAGATTGAAATAGGTCTCTTTGATATCAAAGTCCTTGTGGATGA	6	0.15	No Hit
TGGCAGAAGACTTTAACGACGTTCTTAGAAGACTTATACGCCAGGTATTG	6	0.15	No Hit
GTGGAGTGAGACATGGACACACCCTGGTTCAGGAGCTGCTGAAACTCTGA	5	0.125	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	5	0.125	No Hit
GTCCATGGTGTACACTTTCACTCCCCGGAAGCTGAAGCCCGAACACCGAT	5	0.125	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	5	0.125	No Hit
CCTGGTCCTTCAGCCATCGTCTCCTTAGCTCCCGCAGCTGCTGCAGCCGC	5	0.125	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	5	0.125	No Hit
GATCCAGGTCTGAATGTCTATTGGTTCAGCTTTGGGATAAGCTATGGTTG	5	0.125	No Hit
CCAGGCACACAAACAACAGCATCCCCAGCCTTCCTTTGTTGAACTGCTTC	5	0.125	No Hit
GGAACAGCGTCATTAGCACCTTGCCTACAGCCACCTCCAAGGCCACCTGC	5	0.125	No Hit
TTCTCGTTTTCCAGGTACGTATCCTTCTTGAGCTTGCCAGCCACCAGCCT	5	0.125	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	5	0.125	No Hit
CATCAGAAGACTCGGACACCAGCTTCCCATCTCGGGTTTCAATCTTCTTC	5	0.125	No Hit
TTCTTCTCAAGCAGCTCACGAATCTGCTCCTTTAGGACCTCCACCTCCTC	5	0.125	No Hit
GACATGAGCACGCTGTAGAAGAGCTTCTCATTTCTGTCTTGCAGGTCCAT	5	0.125	No Hit
TCCAAGCGCTGAAGTTCATAGGAGTATGCATTGTTTATACTCCGGAGGTC	5	0.125	No Hit
TCATAGTGGCACCGTCCTTGATCCCCTCCTTGCACAGCCCGAAGTCCGTT	5	0.125	No Hit
GACCATGACACCCTGGCCCAAGTTTTCCTTGTAGAAGCGCTGGGAGGAGA	5	0.125	No Hit
TACTGTCATGGAGACCAGGTTCTCCCCTCGAAGCAACACCAGACCAAGGA	5	0.125	No Hit
AACAGCTCCTTGAGAACACGGTCCTGACTCTGCACGGTGTGCACGATGAT	5	0.125	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	5	0.125	No Hit
CCTATGGGCTGCCTGTGCTGGGACTCCAATCTGAAGGTATCCTTCCTTCT	5	0.125	No Hit
ATTAACAAGCAATCAACCCTCAGCTCAAGATGATAACAATCAAGGTCACA	5	0.125	No Hit
CCTGGCTCAGTGCAGTTCCGCAAATGTAAGTCCTCCTTGGTGAAGCACAG	5	0.125	No Hit
AGGCTGTCCAGCAGCTTGCGGATCTTATGGTTGGTGTTCTCAGCCACCCA	5	0.125	No Hit
GAGACGCAGTTGCACTTCAGCACCTGACAGCCAGCATCTTGAATAGCCTT	5	0.125	No Hit
CGACTGGAGCTTCAGCACTTCTGGTCCACCAAACTCAAACACTCGAATAG	5	0.125	No Hit
GATCCGAAGGCTCCATGTTCCAGATGTCCTTGGCATACTCCTTAATTGTT	5	0.125	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTGCCCACTGGCGATGCATGC	5	0.125	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGTTGCTGAGCATCCCATCC	5	0.125	No Hit
TGAGAAGCCCTTAGCTCCTGGATCTTCTGGGACAGAGTCTTGGTCCAGAA	5	0.125	No Hit
CGCTTCTCCGTGGCCTTACAACACTGCTCAATGCAGCCGGCCATGCCTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275792 spots for SRR25943851.sra
Written 275792 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
Read 275784 spots for SRR25943851.sra
Written 275784 spots for SRR25943851.sra
SRR ids: ['SRR25943851.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_lya9g0c4
SRR25943851.sra spots: 4412552
blocks: [[1, 275784], [275785, 551568], [551569, 827352], [827353, 1103136], [1103137, 1378920], [1378921, 1654704], [1654705, 1930488], [1930489, 2206272], [2206273, 2482056], [2482057, 2757840], [2757841, 3033624], [3033625, 3309408], [3309409, 3585192], [3585193, 3860976], [3860977, 4136760], [4136761, 4412552]]
SRR25943851 file size 1
SRR25943851 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 16 -o SRR25943851 SRR25943851_1.fastq
Input file:	SRR25943851_1.fastq
trimmed:	SRR25943851-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	16
Wed May  6 05:42:37 2026 >> started

Wed May  6 05:42:41 2026 >> done (3.451s)
4412552 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
4412552 (100.00%) reads available; of these:
     84 ( 0.00%) trimmed reads available after processing
4412468 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      2	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      3	  0.00%
 44	      2	  0.00%
 45	      1	  0.00%
 46	     11	  0.00%
 47	     14	  0.00%
 48	     14	  0.00%
 49	     37	  0.00%
 50	4412468	100.00%
4412552 reads passed initial QC


criterion=sequence-density
sequence-density=3.65
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=20
prefix-density=0.00
prefix-fanout=1.0
sequence=CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATGCCT


criterion=fanout-score
sequence-density=1.18
sequence-density-rank=12
fanout-score=11.38
fanout-score-rank=1
prefix-density=1.27
prefix-fanout=10.6
sequence=CCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAATCGGG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 06 05:42:59
                             Started mapping on |	May 06 05:42:59
                                    Finished on |	May 06 05:43:12
       Mapping speed, Million of reads per hour |	1221.94

                          Number of input reads |	4412552
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3648524
                        Uniquely mapped reads % |	82.69%
                          Average mapped length |	49.55
                       Number of splices: Total |	2669
            Number of splices: Annotated (sjdb) |	1115
                       Number of splices: GT/AG |	2429
                       Number of splices: GC/AG |	70
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	169
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.08
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	488741
             % of reads mapped to multiple loci |	11.08%
        Number of reads mapped to too many loci |	168804
             % of reads mapped to too many loci |	3.83%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.41%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	275287	275287	275287
N_multimapping	488741	488741	488741
N_noFeature	1668	3531270	1682
N_ambiguous	151842	65	35964
UnstrandedReadsAssigned:3495014 PositiveStrandReadsAssigned:117189 NegativeStrandReadsAssigned:3610878
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943851 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943851-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,412,552 reads, 4,090,302 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,170 rounds

 109283 SRR25943851.ke.tsv
  52636 SRR25943851.se.tsv
 161919 total
==> SRR25943851.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943851.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943851 completed mapping pipeline successfully
