Starting /dee2/code/volunteer_pipeline.sh SRR25943845
    current disk space = 1266014484
    free memory = 194378404 
SRR25943845 SRAfilesize
10f7da891d3cf6b5a96fb399256f36b6  SRR25943845.sra
SRR25943845.sra file validated
SRR25943845 is single end
SRR25943845 is conventional basespace
SRR25943845 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR25943845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36	32.0	32.0	32.0	32.0	32.0
2	31.46075	32.0	32.0	32.0	32.0	32.0
3	31.501	32.0	32.0	32.0	32.0	32.0
4	31.54975	32.0	32.0	32.0	32.0	32.0
5	31.5545	32.0	32.0	32.0	32.0	32.0
6	35.09	36.0	36.0	36.0	36.0	36.0
7	35.14625	36.0	36.0	36.0	36.0	36.0
8	35.03325	36.0	36.0	36.0	32.0	36.0
9	35.0615	36.0	36.0	36.0	36.0	36.0
10	35.08325	36.0	36.0	36.0	36.0	36.0
11	34.87375	36.0	36.0	36.0	32.0	36.0
12	35.10425	36.0	36.0	36.0	36.0	36.0
13	35.052	36.0	36.0	36.0	36.0	36.0
14	34.983	36.0	36.0	36.0	36.0	36.0
15	34.87025	36.0	36.0	36.0	36.0	36.0
16	34.985	36.0	36.0	36.0	36.0	36.0
17	34.933	36.0	36.0	36.0	32.0	36.0
18	34.79275	36.0	36.0	36.0	32.0	36.0
19	34.9195	36.0	36.0	36.0	36.0	36.0
20	34.98125	36.0	36.0	36.0	36.0	36.0
21	35.00925	36.0	36.0	36.0	36.0	36.0
22	34.9465	36.0	36.0	36.0	36.0	36.0
23	35.0325	36.0	36.0	36.0	36.0	36.0
24	35.03425	36.0	36.0	36.0	36.0	36.0
25	35.09375	36.0	36.0	36.0	36.0	36.0
26	34.92025	36.0	36.0	36.0	36.0	36.0
27	34.9825	36.0	36.0	36.0	36.0	36.0
28	34.8775	36.0	36.0	36.0	32.0	36.0
29	35.054	36.0	36.0	36.0	36.0	36.0
30	34.8565	36.0	36.0	36.0	36.0	36.0
31	35.078	36.0	36.0	36.0	36.0	36.0
32	34.9155	36.0	36.0	36.0	32.0	36.0
33	34.92275	36.0	36.0	36.0	36.0	36.0
34	34.944	36.0	36.0	36.0	32.0	36.0
35	34.9655	36.0	36.0	36.0	36.0	36.0
36	34.90125	36.0	36.0	36.0	36.0	36.0
37	34.86725	36.0	36.0	36.0	32.0	36.0
38	34.888	36.0	36.0	36.0	32.0	36.0
39	34.90275	36.0	36.0	36.0	36.0	36.0
40	34.612	36.0	36.0	36.0	32.0	36.0
41	34.74125	36.0	36.0	36.0	32.0	36.0
42	34.79225	36.0	36.0	36.0	32.0	36.0
43	34.64	36.0	36.0	36.0	32.0	36.0
44	34.67425	36.0	36.0	36.0	32.0	36.0
45	34.51	36.0	36.0	36.0	32.0	36.0
46	34.40225	36.0	36.0	36.0	32.0	36.0
47	34.5315	36.0	36.0	36.0	32.0	36.0
48	34.31275	36.0	36.0	36.0	32.0	36.0
49	34.563	36.0	36.0	36.0	32.0	36.0
50	34.2215	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
11101	1	0.0
11101	2	0.0
11101	3	0.0
11101	4	0.0
11101	5	0.0
11101	6	0.0
11101	7	0.0
11101	8	0.0
11101	9	0.0
11101	10	0.0
11101	11	0.0
11101	12	0.0
11101	13	0.0
11101	14	0.0
11101	15	0.0
11101	16	0.0
11101	17	0.0
11101	18	0.0
11101	19	0.0
11101	20	0.0
11101	21	0.0
11101	22	0.0
11101	23	0.0
11101	24	0.0
11101	25	0.0
11101	26	0.0
11101	27	0.0
11101	28	0.0
11101	29	0.0
11101	30	0.0
11101	31	0.0
11101	32	0.0
11101	33	0.0
11101	34	0.0
11101	35	0.0
11101	36	0.0
11101	37	0.0
11101	38	0.0
11101	39	0.0
11101	40	0.0
11101	41	0.0
11101	42	0.0
11101	43	0.0
11101	44	0.0
11101	45	0.0
11101	46	0.0
11101	47	0.0
11101	48	0.0
11101	49	0.0
11101	50	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	3.0
25	3.0
26	9.0
27	30.0
28	44.0
29	66.0
30	96.0
31	138.0
32	172.0
33	286.0
34	605.0
35	2546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.35	16.925	27.875	29.849999999999998
2	27.425	19.05	28.199999999999996	25.324999999999996
3	40.675	20.45	18.3	20.575
4	31.874999999999996	19.05	19.375	29.7
5	16.425	28.525	25.575	29.475
6	34.15061295971979	21.56617463097323	25.444083062296723	18.839129347010257
7	35.575	16.325	30.175	17.925
8	21.349999999999998	17.45	21.775	39.425
9	20.375	19.8	32.824999999999996	27.0
10	26.85	16.45	27.1	29.599999999999998
11	26.35	36.975	16.075	20.599999999999998
12	37.15	21.9	18.975	21.975
13	35.199999999999996	28.425	18.025	18.35
14	17.0	18.7	25.35	38.95
15	17.825	33.175	20.8	28.199999999999996
16	15.024999999999999	33.324999999999996	23.45	28.199999999999996
17	19.45	22.95	28.425	29.175
18	20.95	16.575	36.9	25.575
19	20.724999999999998	29.425	25.174999999999997	24.675
20	33.550000000000004	14.249999999999998	24.05	28.15
21	16.775000000000002	18.05	34.525	30.65
22	17.45	19.425	37.325	25.8
23	33.95	16.650000000000002	19.900000000000002	29.5
24	27.650000000000002	15.075	25.724999999999998	31.55
25	0.975	3.5000000000000004	85.275	10.25
26	13.675	25.3	19.775000000000002	41.25
27	28.749999999999996	25.25	23.849999999999998	22.15
28	14.2	20.974999999999998	33.324999999999996	31.5
29	33.375	26.625	12.675	27.325
30	25.974999999999998	19.475	22.8	31.75
31	37.1	25.6	13.5	23.799999999999997
32	27.1	20.849999999999998	19.7	32.35
33	27.975	21.7	16.8	33.525
34	22.175	20.375	34.225	23.225
35	25.924999999999997	18.425	33.5	22.15
36	35.275	10.975	30.599999999999998	23.150000000000002
37	23.799999999999997	36.1	21.2	18.9
38	33.375	10.95	18.775	36.9
39	35.8	23.400000000000002	17.95	22.85
40	18.6	28.575	23.45	29.375
41	18.925	32.35	18.325	30.4
42	33.324999999999996	15.950000000000001	26.875	23.849999999999998
43	28.825	21.2	21.349999999999998	28.625
44	27.425	15.25	22.3	35.025
45	12.225	29.549999999999997	18.3	39.925
46	17.125	28.475	35.075	19.325
47	26.55	15.2	15.275	42.975
48	19.6	22.525000000000002	31.75	26.125
49	31.874999999999996	22.525000000000002	23.75	21.85
50	15.75	24.05	23.0	37.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	2.5
30	5.0
31	2.5
32	0.0
33	0.0
34	0.0
35	4.5
36	9.0
37	20.5
38	32.0
39	49.0
40	66.0
41	112.0
42	158.0
43	198.5
44	239.0
45	230.5
46	222.0
47	191.0
48	160.0
49	455.5
50	751.0
51	677.5
52	604.0
53	429.5
54	255.0
55	306.5
56	358.0
57	372.0
58	386.0
59	415.5
60	445.0
61	297.0
62	149.0
63	108.5
64	68.0
65	58.0
66	48.0
67	32.5
68	17.0
69	12.5
70	8.0
71	11.0
72	14.0
73	9.5
74	5.0
75	3.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.075
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12599681020734	22.925
2	10.685805422647528	6.7
3	5.103668261562999	4.8
4	2.3923444976076556	3.0
5	1.3556618819776716	2.125
6	1.4354066985645932	2.7
7	0.9569377990430622	2.1
8	0.3189792663476874	0.8
9	0.4784688995215311	1.35
>10	3.349282296650718	22.975
>50	0.5582137161084529	11.55
>100	0.23923444976076555	18.975
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGT	318	7.95	No Hit
TGGCCTGTTGAAACACACAGCTGGTAATACGGCCTTACGAATGCCACCAA	289	7.225	No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA	152	3.8	No Hit
CTGCGGCCATGTCTGTTGGCTTCCTGGTCAGCAATGGTGTCCTCATGTCC	80	2.0	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCC	78	1.95	No Hit
AGGACTTGGTGGATCAGCAGCAGGTCCAGCAAGTTGTAATCGGCAAAGGA	75	1.875	No Hit
CCACAGAGGCCTGTATCTTCTCCATCAGGTTTGCCCACTGGCGATGCATG	72	1.7999999999999998	No Hit
GATTTGGTGTCCAGTTCTACTCTGTACACTCCTTCTACAAACTTCTCATC	53	1.325	No Hit
GGTACTTGCCGGAGAAGTTCATGGTGGCAACGAGGCTTCCTTTCCACAGC	53	1.325	No Hit
CCACAGACAAGATCTCCTGGCACTTTTCACACTGGCCCTTCATCTTCAGG	51	1.275	No Hit
TTGATGGATTTCGCAGGCCATTCCCCTAAACATCATTGGGCAGGAGCACA	45	1.125	No Hit
CTACTTCTCAGAGGTCGGGCAGCCCAGCCAATACTGAGCAATGGAGCGTG	44	1.0999999999999999	No Hit
AACAAGTTCCGCAGGGTGCTGAGCTCTCGCGACAGCTGCTCCACCTTCTT	40	1.0	No Hit
TCCCCCGAGCATGGAAGTATTTGTCTCCATCTTTCCAGCCAGCTTCCTTC	40	1.0	No Hit
TCTGTGAGACAGGTGGAGAGCAGATCACAGACCTTGGCATGGGAGTCTGT	40	1.0	No Hit
GTATTTCATGAGAATCAGGAGCCCATATCTCAGAGTTGTGCTGGTGGTCT	39	0.975	No Hit
ATCCACCAGCCGGATCCATCCTGTTCCGCACAGTTGCCTTCAAACTTATC	34	0.8500000000000001	No Hit
TAGGAGTGCATGTCTCCAAAGTCCTTCTCGAAGAAGACGTAGGCCTTGCT	33	0.8250000000000001	No Hit
CCTCTAGCAGCTCCCGCTCAGTCTTATTGGAGGTCCCGTAGGTGAAGATG	31	0.775	No Hit
GCCTTATACACCTCAATTAGCAGGTCCTTCACGTACTGGATCTCCCGCTC	31	0.775	No Hit
ACAGCGGCCGTGCAGATCCCGTTCTCTCGGACAGCGCCTTCCTCCCAAGC	29	0.7250000000000001	No Hit
TCTTTCCTCCGGTTCCAGCGCTCCTTAAGCGCATATTTCAGCATCTTGTC	26	0.65	No Hit
TCATTGTAGAGTGAAAGTGGAGACTCCCGACCGAGGATGTACACTTGGCC	25	0.625	No Hit
GCTGAAGCTGAAGGTTCTGTAGTTTCATGGCCAGTCCCATGTTACCAGCA	24	0.6	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTAG	22	0.5499999999999999	No Hit
TCCACATCCTCTTTCCATTTCTTCTGGAATTCGTCCAGGTAGGGCTGCAC	22	0.5499999999999999	No Hit
CTGTTGTTCAGATACTCGACGCGGTTGCACACCTTGTTCTCAATCACAGG	22	0.5499999999999999	No Hit
TTCTTCTGAATGAAAAGCTGAGCGTCCTTCAGGTGGCCGGCAATGTTCTC	22	0.5499999999999999	No Hit
TCAGCACACCGGCACATTTCACTGTGGCACAGCTTGCTGAGCATCCCATC	21	0.525	No Hit
AATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGG	21	0.525	No Hit
TGTAGATCTGAGGCTCAGGCTTGATCATCCCAACCTGACAGGACTCTATC	20	0.5	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCAC	19	0.475	No Hit
CGTGCACAGCATTTGCTTGTTTCCTTGCGACTTCAGCCACTGCATTCCCA	18	0.44999999999999996	No Hit
GTGAACGCCACTTTGTCCACACCCTCATGGGATGCGATGGCAGCCCCGGC	18	0.44999999999999996	No Hit
CCCTCATGGTTCACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	17	0.42500000000000004	No Hit
TCCCCTCCTTTCTGGCTGATCCCATAGAGAGAATATAAGAGACACAGGTT	17	0.42500000000000004	No Hit
GGCTCCTCTGTCACATTCAGCCGCTCGTCGGAGTACAGGATGCTAGCTGA	16	0.4	No Hit
CCCAAGTCGTGGATGCAGAGTTATTCATACCCAGCACTCCCCAGTCACAT	16	0.4	No Hit
TCCTCCTGGTACTCAGGAACAATCTGGTCTGAAGTTCCTATTTCTGAGTC	16	0.4	No Hit
GGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCAC	15	0.375	No Hit
TTCCACACATGTGCAAGCTGCTCCTTCACAGACTTTCTGAAGCCTGGTGT	14	0.35000000000000003	No Hit
GGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCG	13	0.325	No Hit
AGCAGAGACTTGGCACGAATGTTGTAGTTGAGGCACGCCATGGGACAGCC	13	0.325	No Hit
TCAGTTACAGCAGCACTGGTGTCATCCACAGTAGGATCATCCTCATCAAT	12	0.3	No Hit
GATGAGACTCTGGACAGGCAGCAGTTGCTCAGTGGTCACGACTTTCTGAG	11	0.27499999999999997	No Hit
GATATTTGTCCTCATGGAAGGACGTAGCCTATAAATGCTGTGGCTATGAC	11	0.27499999999999997	No Hit
TCATCATCATGTCCATCATGAGCCTCAACAGCTGCAGTTCCAAGGACCAG	11	0.27499999999999997	No Hit
AGAAATCATGCGCACGATGGCTCTTTTCTGTGGGTCCTGAGGCAGGAGCC	11	0.27499999999999997	No Hit
CGGATGAGCACGGCGATTGGTACGTTGTGCACCGTGGCGATGCAGCCCGA	10	0.25	No Hit
ATGAACTGCCCTTCAGTCGCCTCGTCCGTGATGCCTAGGAAGGCAATGCC	10	0.25	No Hit
ATGCACCAGCCACCTTACAGCCCATGATCACTGCCAGTCCAACTCCTCCC	10	0.25	No Hit
ATCTGTCCTTGAGCCTTCTCGGCTTTTACCCAGGTCCTCTTCCACTGTGC	10	0.25	No Hit
CGTCTCCACGTTGCGTTGTTTGGCTTTATCTCGGCTCTTGCGCACCGCGA	9	0.22499999999999998	No Hit
GGCAACACCTTCAAGCTCTGGATGTTCTTGAGGTGAATCCCAGCCATGGT	9	0.22499999999999998	No Hit
GCGTATCCTGTGGTCGTAGCATCAGCTAGTAGGACAGACGATAGCGGAAT	9	0.22499999999999998	No Hit
CCCTGATGGTACACATGGCATGTGTAATACTGCTCCTTCCCAAGAGGCAC	9	0.22499999999999998	No Hit
GCGCGTGTCGTTCTGTGAGAACATCTTGTTTCGGTTGGCGACCAGCCAGC	9	0.22499999999999998	No Hit
CACAAAGTCCCACATGTTCACATCTCCGATGTCTCCCACCAAAGACTGCT	9	0.22499999999999998	No Hit
AGTGAGAATCCACAGAAGCGCCAATCACTTGGCAGTTGAGTTTCTTAAAT	8	0.2	No Hit
AACAGAAGTTCTTCCTTGAGTGCCTCGATTTCTGTCTCCAGCTGCAGCCT	8	0.2	No Hit
ATGACGTCAGCAAAGCGTTGCACTTCGTGGATCACTGCATCTGTGTAAGG	8	0.2	No Hit
GATGCTTCAGTTTGGCAGCCTTCATAGTGTAGTAAGTGTACATGACAGAA	8	0.2	No Hit
AGGCTTGACGATGGTGGTGATGGCTCCTGGCAGGAGCTTGTAGGATTCAG	7	0.17500000000000002	No Hit
GAGGTATCCTAGGTAACATGTGGATATACTGGTATTGGACTTCTCAGTGA	7	0.17500000000000002	No Hit
GTGCCAGAAGAGGTGACTTTCTCCTTTCCAATCAGGAGCTCTTTATCTCC	7	0.17500000000000002	No Hit
ATGGTGACGGCTCCGCAAGCGCGGCCGCTCCAGGTCGCCGAGACGAACTG	7	0.17500000000000002	No Hit
CACCCATGACCATGAGAAATCTGCTCAGCGAGGAGTAGAAGTAAAGGATG	7	0.17500000000000002	No Hit
TCAGCCAGGCCGTTCCAATGCTGCTGGTGTTGGAGGCAGCGGAGCAGCAC	7	0.17500000000000002	No Hit
AAACAGGCCAGCGTAGTGTTCCAGTCTCCGTTGCCAGTGGCTGCAGCCTT	7	0.17500000000000002	No Hit
GTGTGCAGCAAGGTCACTAGAATCCTGCAGCCAGATGCTCCCAGAGGATG	7	0.17500000000000002	No Hit
ACATGACGGCGATGATATCTGAGTTCTGGATAGTAGACAAGCGATGAGCA	7	0.17500000000000002	No Hit
CTCCTTCTGCTCATCACACAGGTATTTCTGCAGCAGTCTTGCTCGCACTT	7	0.17500000000000002	No Hit
ACCTTGACTGTGACTTTAGGTCCCTTCTTCTTATCGTTGGCCACGGAGGG	7	0.17500000000000002	No Hit
CCACCCGTTCCCCCAAGAGCAGGTGGTGGGCAGGTCCCTTGACCTCTGCC	7	0.17500000000000002	No Hit
CTATGTCATAGACACGGCGACTTGTTCGATGAAGCTGGCCAGGTTTATAT	6	0.15	No Hit
AGGAGTGCTGAGAGCTTCTCTGCATATTCATGCATCGGAGAGTGGAAGAA	6	0.15	No Hit
CCTGGACCCTGGTAGACTGCCTTCTCCAAGCCGTCCACCACTTTCATGTA	6	0.15	No Hit
AACTGAAAATCTTCTTTGCTGACTCAACACATTTTGCGTCATCAAAAGGC	6	0.15	No Hit
TGTAGATGCGGAAGATGTCATCTTTCACATAGGACATAGCCATTGTCAGC	6	0.15	No Hit
GCATCATACATCTCCTTCAGGATGTGTGGGATCTTGGAGATCAGCTGAGC	6	0.15	No Hit
AGTGCTATGGCTGGTCAGCCGAAGCCCCTCTCCTGTCCTCAGGATCCTCC	6	0.15	No Hit
TGTCACAGTATACTCTGTACGGCTTGATGGAGGTGTCAGGCTGGATGAGA	6	0.15	No Hit
GTGCTTGATCTCACAATCTTCTGCCAGCTCTGTGTTACTTTCCTTGGAGC	6	0.15	No Hit
ATGTAGGAGAGGCTTGTGAACATGTAACTGTTAACAGCCAACACCACGGA	6	0.15	No Hit
GAGTTGTCTAGCTCTTCTGTCAGGTTGTCCAACATTGGAATTTCCAGAAA	6	0.15	No Hit
GACTGCAGTGGCCACCACACAGACTAGTAGGAATCCAATCACATCCAGAG	6	0.15	No Hit
GCGAGTCCTTGTAGCCTACGATGGCCGCGTCCTGACAGGTCCCGTCCGCC	6	0.15	No Hit
AAACGAGCCACCGCCATCCCATTCTTGAAATGTTTCTCCATCCGGACCTG	6	0.15	No Hit
GGCAGCACAGACACTGTTGCTACGTAGATAAATCCGCCTGCAGTGAATGG	6	0.15	No Hit
CCCTGATGGTACACATGGCATGTGTAATTCTGCTCCTTCCCAAGAGGCAC	6	0.15	No Hit
AAGATGTGTTCCTCAGTGTTGCACTTGTATGAGTTTCCCACAGTGGCCTG	6	0.15	No Hit
TCATAGTGCACCACACACTTGTCCTGGTCGGCCACAGGCAGCATGACATA	6	0.15	No Hit
GACACAACATCTCTTGATGCCAGGTCCTTGGCAACAGGTGCGTATCTCTC	5	0.125	No Hit
AGTTATGGCTGGAAGGCCAGTGCTTCATCTCAGGGTGTCGAACAAACAGC	5	0.125	No Hit
ATGAGCCATAAGAAAGTTATCATAGTCTGTCTTAGGTATAGTAAATGTAT	5	0.125	No Hit
TGTGCCATCATTGAAGAGCACGGCTACACGGCGGCTGGACAGCTGATAGC	5	0.125	No Hit
CACAAAGTACCCAGCATGAGCCCTAGGAGAAAGAGGAAGACATCAAGCAT	5	0.125	No Hit
CATCAGAAGACTCGGACACCAGCTTCCCATCTCGGGTTTCAATCTTCTTC	5	0.125	No Hit
CTTTAGTCCAGGCCACTTCAGTCATCAGCAAGCGGAGGTAGTACATGGCA	5	0.125	No Hit
AAACGCCCACTTTGACATAGACTTTATAGACCATACCACCAATACCGAAA	5	0.125	No Hit
CAGGAGTAGGAGAGCATATCTCAGTGTTGTGCTTGTGGTCTCTGTCCCTG	5	0.125	No Hit
GTTGCTAAGCTTCCAACCAAACTCTCCTGCTCCTCAATTTCCTGGTATTC	5	0.125	No Hit
TCTCGAACCACATCTCCGCACCGTTACTCGCACGCCAACCCGTCACCGGC	5	0.125	No Hit
CCTGAAGGCCTCTAGGCCCTTGTATAAGTTGTTCTGTTTGTCACTGCTGG	5	0.125	No Hit
GCTGAGGCTGAATGCATGTCCTGGTTTTCAAAAGGTGGCAGTGGCTCTCT	5	0.125	No Hit
TGGTTAATCTGCTAGTATCACCCATTTGCACTGTCTTGAGGTTACAGCAT	5	0.125	No Hit
GACTCAATAGCTGGAGTTGGTTGGTCCTCAGGGTTAGAATCCCAGAAGCC	5	0.125	No Hit
AAGGAGGCCCGATTCTCTTCTACCCAAGACTTCACTCTCACACGGCGTTC	5	0.125	No Hit
ATCCTTGGATCCCTGGAGCGTCGCCCTCGCTCAGCCTCCCGCGCCCACCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGACA	20	6.952051E-4	44.000004	5
CAGCCAA	20	6.952051E-4	44.000004	25
AGCCCAG	20	6.952051E-4	44.000004	21
GAGGTCG	20	6.952051E-4	44.000004	11
GGAGAGC	20	6.952051E-4	44.000004	15
GAGCGTG	20	6.952051E-4	44.000004	44
TGTTGAA	45	2.728484E-10	44.000004	6
ATGGGAG	20	6.952051E-4	44.000004	39
CAATGGA	20	6.952051E-4	44.000004	39
GTTGAAA	45	2.728484E-10	44.000004	7
CCAATAC	20	6.952051E-4	44.000004	28
GGCATGG	20	6.952051E-4	44.000004	36
GCCTTAC	45	2.728484E-10	44.000004	32
CTGAGCA	20	6.952051E-4	44.000004	34
GGGCAGC	20	6.952051E-4	44.000004	17
GCAAGTT	20	6.952051E-4	44.000004	29
GCCCAGC	20	6.952051E-4	44.000004	22
CACACAG	45	2.728484E-10	44.000004	14
CAGCAGG	20	6.952051E-4	44.000004	18
CAGCAAG	20	6.952051E-4	44.000004	27
>>END_MODULE
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
Read 475169 spots for SRR25943845.sra
Written 475169 spots for SRR25943845.sra
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
Read 475162 spots for SRR25943845.sra
Written 475162 spots for SRR25943845.sra
SRR ids: ['SRR25943845.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_06ce5ch1
SRR25943845.sra spots: 3801303
blocks: [[1, 475162], [475163, 950324], [950325, 1425486], [1425487, 1900648], [1900649, 2375810], [2375811, 2850972], [2850973, 3326134], [3326135, 3801303]]
SRR25943845 file size 656700
SRR25943845 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR25943845 SRR25943845_1.fastq
Input file:	SRR25943845_1.fastq
trimmed:	SRR25943845-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue May  5 05:37:36 2026 >> started

Tue May  5 05:37:38 2026 >> done (1.234s)
3801303 reads processed; of these:
      0 ( 0.00%) short reads filtered out after trimming by size control
      0 ( 0.00%) empty reads filtered out after trimming by size control
3801303 (100.00%) reads available; of these:
     94 ( 0.00%) trimmed reads available after processing
3801209 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 38	      1	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      4	  0.00%
 44	      1	  0.00%
 45	      1	  0.00%
 46	     10	  0.00%
 47	      9	  0.00%
 48	     30	  0.00%
 49	     38	  0.00%
 50	3801209	100.00%
3801303 reads passed initial QC


criterion=sequence-density
sequence-density=9.42
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=16
prefix-density=0.05
prefix-fanout=1.0
sequence=CTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATCTGTCCTG


criterion=fanout-score
sequence-density=0.43
sequence-density-rank=38
fanout-score=29.57
fanout-score-rank=1
prefix-density=12.56
prefix-fanout=1.0
sequence=CAGCGATCTGTAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	May 05 05:37:44
                             Started mapping on |	May 05 05:37:44
                                    Finished on |	May 05 05:37:51
       Mapping speed, Million of reads per hour |	1954.96

                          Number of input reads |	3801303
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2723999
                        Uniquely mapped reads % |	71.66%
                          Average mapped length |	49.56
                       Number of splices: Total |	1920
            Number of splices: Annotated (sjdb) |	564
                       Number of splices: GT/AG |	1660
                       Number of splices: GC/AG |	14
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	246
                      Mismatch rate per base, % |	0.22%
                         Deletion rate per base |	0.08%
                        Deletion average length |	1.07
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	422745
             % of reads mapped to multiple loci |	11.12%
        Number of reads mapped to too many loci |	556680
             % of reads mapped to too many loci |	14.64%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.57%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	654559	654559	654559
N_multimapping	422745	422745	422745
N_noFeature	15676	2641094	15691
N_ambiguous	103985	15	21884
UnstrandedReadsAssigned:2604338 PositiveStrandReadsAssigned:82890 NegativeStrandReadsAssigned:2686424
Dataset is classified negative stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR25943845 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR25943845-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,801,303 reads, 3,543,789 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,143 rounds

 109283 SRR25943845.ke.tsv
  52636 SRR25943845.se.tsv
 161919 total
==> SRR25943845.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR25943845.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR25943845 completed mapping pipeline successfully
