Starting /dee2/code/volunteer_pipeline.sh SRR31990351
    current disk space = 36247672
    free memory = 194931084 
SRR31990351 SRAfilesize
3ad46ca887d70125f053fd23f5ffd7e6  SRR31990351.sra
SRR31990351.sra file validated
SRR31990351 is paired end
SRR31990351 is conventional basespace
SRR31990351 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990351_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1175	37.0	37.0	37.0	37.0	37.0
2	35.7415	37.0	37.0	37.0	37.0	37.0
3	36.249	37.0	37.0	37.0	37.0	37.0
4	36.1805	37.0	37.0	37.0	37.0	37.0
5	36.4335	37.0	37.0	37.0	37.0	37.0
6	36.163	37.0	37.0	37.0	37.0	37.0
7	36.2345	37.0	37.0	37.0	37.0	37.0
8	36.4225	37.0	37.0	37.0	37.0	37.0
9	36.3515	37.0	37.0	37.0	37.0	37.0
10-14	36.2964	37.0	37.0	37.0	37.0	37.0
15-19	36.19539999999999	37.0	37.0	37.0	37.0	37.0
20-24	36.2325	37.0	37.0	37.0	37.0	37.0
25-29	35.9936	37.0	37.0	37.0	37.0	37.0
30-34	36.0343	37.0	37.0	37.0	37.0	37.0
35-39	36.0675	37.0	37.0	37.0	37.0	37.0
40-44	35.9106	37.0	37.0	37.0	37.0	37.0
45-49	35.857600000000005	37.0	37.0	37.0	37.0	37.0
50-54	35.8382	37.0	37.0	37.0	37.0	37.0
55-59	35.8015	37.0	37.0	37.0	37.0	37.0
60-64	35.778099999999995	37.0	37.0	37.0	37.0	37.0
65-69	35.657300000000006	37.0	37.0	37.0	37.0	37.0
70-74	35.5981	37.0	37.0	37.0	34.6	37.0
75-79	35.600300000000004	37.0	37.0	37.0	37.0	37.0
80-84	35.6087	37.0	37.0	37.0	37.0	37.0
85-89	35.55400000000001	37.0	37.0	37.0	37.0	37.0
90-94	35.4553	37.0	37.0	37.0	37.0	37.0
95-99	35.38459999999999	37.0	37.0	37.0	37.0	37.0
100-104	35.2824	37.0	37.0	37.0	34.6	37.0
105-109	35.0867	37.0	37.0	37.0	32.2	37.0
110-114	34.9336	37.0	37.0	37.0	25.0	37.0
115-119	34.9214	37.0	37.0	37.0	27.4	37.0
120-124	34.759499999999996	37.0	37.0	37.0	25.0	37.0
125-129	34.7492	37.0	37.0	37.0	25.0	37.0
130-134	34.6036	37.0	37.0	37.0	25.0	37.0
135-139	34.555099999999996	37.0	37.0	37.0	25.0	37.0
140-144	34.4488	37.0	37.0	37.0	25.0	37.0
145-149	33.884499999999996	37.0	37.0	37.0	22.2	37.0
150	34.174	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	5.0
22	6.0
23	9.0
24	13.0
25	13.0
26	14.0
27	28.0
28	29.0
29	33.0
30	53.0
31	73.0
32	143.0
33	176.0
34	245.0
35	624.0
36	2435.0
37	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	59.52499999999999	9.725	5.925	24.825
2	20.917849898580123	18.940162271805274	35.218052738336716	24.92393509127789
3	19.225	28.299999999999997	23.599999999999998	28.875
4	7.625	10.424999999999999	42.075	39.875
5	10.475	40.925	37.225	11.375
6	33.900000000000006	41.949999999999996	15.4	8.75
7	28.599999999999998	31.225	21.575	18.6
8	28.225	33.375	19.650000000000002	18.75
9	27.35	11.774999999999999	12.049999999999999	48.825
10-14	22.84	22.54	31.755	22.865
15-19	23.64	28.875	24.535	22.95
20-24	25.295	25.3	24.94	24.465
25-29	23.025000000000002	25.669999999999998	25.89	25.415
30-34	24.610000000000003	26.484999999999996	25.46	23.445
35-39	23.515	26.85	25.44	24.195
40-44	23.785	27.334999999999997	24.735	24.145
45-49	24.515	25.11	25.88	24.495
50-54	23.415	25.28	27.060000000000002	24.245
55-59	23.905	26.26	25.695	24.14
60-64	23.855	26.450000000000003	25.455	24.240000000000002
65-69	24.6	25.759999999999998	25.55	24.09
70-74	23.91	24.79	26.66	24.64
75-79	24.884999999999998	26.86	24.884999999999998	23.369999999999997
80-84	24.43	26.284999999999997	24.575	24.709999999999997
85-89	25.15	25.119999999999997	24.75	24.98
90-94	24.34	25.755	25.83	24.075
95-99	25.069999999999997	25.31	26.105	23.515
100-104	25.064999999999998	25.14	25.869999999999997	23.925
105-109	23.655	24.6	25.985000000000003	25.759999999999998
110-114	25.255	24.884999999999998	24.490000000000002	25.369999999999997
115-119	24.805	24.54	26.200000000000003	24.455
120-124	24.965	25.264999999999997	25.314999999999998	24.455
125-129	25.965	24.98	25.705	23.35
130-134	26.064999999999998	24.19	25.245	24.5
135-139	26.090000000000003	24.455	24.94	24.515
140-144	25.635	24.34	25.855	24.169999999999998
145-149	26.215	23.919999999999998	25.36	24.505
150	26.55	26.875	24.125	22.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	2.0
15	2.5
16	2.0
17	2.0
18	0.5
19	0.5
20	1.0
21	0.5
22	1.5
23	4.5
24	7.0
25	9.0
26	18.0
27	27.5
28	29.0
29	24.0
30	21.0
31	19.0
32	25.5
33	47.0
34	65.5
35	66.5
36	67.5
37	78.0
38	82.5
39	87.5
40	114.0
41	114.5
42	95.5
43	95.5
44	100.5
45	100.0
46	106.5
47	123.5
48	122.5
49	152.5
50	146.0
51	129.5
52	185.0
53	210.5
54	212.0
55	216.0
56	191.0
57	160.0
58	161.0
59	156.0
60	115.5
61	80.0
62	57.0
63	39.5
64	38.5
65	36.0
66	19.5
67	10.0
68	7.5
69	4.5
70	2.0
71	1.0
72	1.5
73	1.5
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.4000000000000001
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.35657446002273	53.675
2	10.003789314134142	13.200000000000001
3	3.031451307313376	6.0
4	1.6672982190223569	4.3999999999999995
5	1.0610079575596816	3.5000000000000004
6	0.5305039787798408	2.1
7	0.7957559681697612	3.675
8	0.34103827207275483	1.7999999999999998
9	0.2652519893899204	1.575
>10	0.9473285335354301	10.075000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	41	1.0250000000000001	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	34	0.8500000000000001	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	26	0.65	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	25	0.625	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	20	0.5	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	19	0.475	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	18	0.44999999999999996	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	16	0.4	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	16	0.4	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	15	0.375	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	15	0.375	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	14	0.35000000000000003	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	13	0.325	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	12	0.3	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	12	0.3	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	12	0.3	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	11	0.27499999999999997	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	11	0.27499999999999997	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	11	0.27499999999999997	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	11	0.27499999999999997	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	11	0.27499999999999997	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	10	0.25	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	10	0.25	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	10	0.25	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	10	0.25	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	9	0.22499999999999998	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	9	0.22499999999999998	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	9	0.22499999999999998	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	9	0.22499999999999998	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	9	0.22499999999999998	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	9	0.22499999999999998	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	9	0.22499999999999998	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	8	0.2	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	8	0.2	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	8	0.2	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	8	0.2	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	8	0.2	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	8	0.2	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	8	0.2	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	8	0.2	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	8	0.2	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	7	0.17500000000000002	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	7	0.17500000000000002	No Hit
TTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGATATAGTA	7	0.17500000000000002	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	7	0.17500000000000002	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	7	0.17500000000000002	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	7	0.17500000000000002	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	7	0.17500000000000002	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	7	0.17500000000000002	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	7	0.17500000000000002	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	7	0.17500000000000002	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	7	0.17500000000000002	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	7	0.17500000000000002	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	7	0.17500000000000002	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	7	0.17500000000000002	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	7	0.17500000000000002	No Hit
GCTTAGAGGTGGGTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTA	7	0.17500000000000002	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	7	0.17500000000000002	No Hit
GCCTAACACTTCCATCTTCCCTGTTTCCAACTCATGCCTTCAAACGTACA	7	0.17500000000000002	No Hit
GTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCCCTACAATCACCACG	7	0.17500000000000002	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	7	0.17500000000000002	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	7	0.17500000000000002	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	6	0.15	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	6	0.15	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	6	0.15	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	6	0.15	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	6	0.15	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	6	0.15	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	6	0.15	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	6	0.15	No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT	6	0.15	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	6	0.15	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	6	0.15	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	6	0.15	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	6	0.15	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	6	0.15	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	5	0.125	No Hit
CTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTGTCCATCTGC	5	0.125	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	5	0.125	No Hit
CTACAAACACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGC	5	0.125	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	5	0.125	No Hit
GGACAGGAAAGACCCTCCGCAAAGGGTCTTTGTCAGACAAGCAGGAAGCT	5	0.125	No Hit
ACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCCTC	5	0.125	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	5	0.125	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	5	0.125	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	5	0.125	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	5	0.125	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	5	0.125	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	5	0.125	No Hit
GGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAG	5	0.125	No Hit
GTACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	5	0.125	No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT	5	0.125	No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA	5	0.125	No Hit
GGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGCAGTGACAA	5	0.125	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	5	0.125	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	5	0.125	No Hit
CCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGGTCCTCGTTGTCGTGG	5	0.125	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	5	0.125	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	5	0.125	No Hit
GGACATTACAACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTC	5	0.125	No Hit
GTACATGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACAT	5	0.125	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	5	0.125	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	5	0.125	No Hit
CTCCACACCCACCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.025	0.0
38-39	0.0	0.0	0.0	0.05	0.0
40-41	0.0	0.0	0.0	0.0875	0.0
42-43	0.0	0.0	0.0	0.225	0.0
44-45	0.0	0.0	0.0	0.2625	0.0
46-47	0.0	0.0	0.0	0.4	0.0
48-49	0.0	0.0	0.0	0.5	0.0
50-51	0.0	0.0	0.0	0.5625	0.0
52-53	0.0	0.0	0.0	0.6625	0.0
54-55	0.0	0.0	0.0	0.7375	0.0
56-57	0.0	0.0	0.0	0.925	0.0
58-59	0.0	0.0	0.0	1.0375	0.0
60-61	0.0	0.0	0.0	1.1749999999999998	0.0
62-63	0.0	0.0	0.0	1.25	0.0
64-65	0.0	0.0	0.0	1.3625	0.0
66-67	0.0	0.0	0.0	1.525	0.0
68-69	0.0	0.0	0.0	1.6625	0.0
70-71	0.0	0.0	0.0	1.8125	0.0
72-73	0.0	0.0	0.0	1.9125	0.0
74-75	0.0	0.0	0.0	2.0125	0.0
76-77	0.0	0.0	0.0	2.25	0.0
78-79	0.0	0.0	0.0	2.3375	0.0
80-81	0.0	0.0	0.0	2.5374999999999996	0.0
82-83	0.0	0.0	0.0	2.8	0.0
84-85	0.0	0.0	0.0	2.925	0.0
86-87	0.0	0.0	0.0	3.0875	0.0
88-89	0.0	0.0	0.0	3.2625	0.0
90-91	0.0	0.0	0.0	3.4875	0.0
92-93	0.0	0.0	0.0	3.7249999999999996	0.0
94-95	0.0	0.0	0.0	3.95	0.0
96-97	0.0	0.0	0.0	4.1625	0.0
98-99	0.0	0.0	0.0	4.375	0.0
100-101	0.0	0.0	0.0	4.637499999999999	0.0
102-103	0.0	0.0	0.0	4.987500000000001	0.0
104-105	0.0	0.0	0.0	5.2125	0.0
106-107	0.0	0.0	0.0	5.7125	0.0
108-109	0.0	0.0	0.0	6.012499999999999	0.0
110-111	0.0	0.0	0.0	6.1875	0.0
112-113	0.0	0.0	0.0	6.550000000000001	0.0
114-115	0.0	0.0	0.0	7.074999999999999	0.0
116-117	0.0	0.0	0.0	7.45	0.0
118-119	0.0	0.0	0.0	7.8125	0.0
120-121	0.0	0.0	0.0	8.1375	0.0
122-123	0.0	0.0	0.0	8.5375	0.0
124-125	0.0	0.0	0.0	8.975000000000001	0.0
126-127	0.0	0.0	0.0	9.3125	0.0
128-129	0.0	0.0	0.0	9.7125	0.0
130-131	0.0	0.0	0.0	10.3	0.0
132-133	0.0	0.0	0.0	10.8125	0.0
134-135	0.0	0.0	0.0	11.337499999999999	0.0
136-137	0.0	0.0	0.0	11.9	0.0
138	0.0	0.0	0.0	12.15	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTGGA	10	0.006716492	145.79747	1
CTCTTTC	10	0.0069772652	143.975	8
>>END_MODULE
SRR31990351 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990351_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.692	37.0	37.0	37.0	37.0	37.0
2	36.1905	37.0	37.0	37.0	37.0	37.0
3	36.168	37.0	37.0	37.0	37.0	37.0
4	36.033	37.0	37.0	37.0	37.0	37.0
5	36.2955	37.0	37.0	37.0	37.0	37.0
6	36.2415	37.0	37.0	37.0	37.0	37.0
7	36.1825	37.0	37.0	37.0	37.0	37.0
8	36.2335	37.0	37.0	37.0	37.0	37.0
9	36.2325	37.0	37.0	37.0	37.0	37.0
10-14	36.1732	37.0	37.0	37.0	37.0	37.0
15-19	36.1463	37.0	37.0	37.0	37.0	37.0
20-24	36.1091	37.0	37.0	37.0	37.0	37.0
25-29	36.1815	37.0	37.0	37.0	37.0	37.0
30-34	36.0559	37.0	37.0	37.0	37.0	37.0
35-39	36.0664	37.0	37.0	37.0	37.0	37.0
40-44	35.8841	37.0	37.0	37.0	37.0	37.0
45-49	35.8875	37.0	37.0	37.0	37.0	37.0
50-54	35.8318	37.0	37.0	37.0	37.0	37.0
55-59	35.7751	37.0	37.0	37.0	37.0	37.0
60-64	35.80749999999999	37.0	37.0	37.0	37.0	37.0
65-69	35.739900000000006	37.0	37.0	37.0	37.0	37.0
70-74	35.556	37.0	37.0	37.0	37.0	37.0
75-79	35.675	37.0	37.0	37.0	37.0	37.0
80-84	35.5303	37.0	37.0	37.0	34.6	37.0
85-89	35.4912	37.0	37.0	37.0	34.6	37.0
90-94	35.3504	37.0	37.0	37.0	34.6	37.0
95-99	35.3521	37.0	37.0	37.0	32.2	37.0
100-104	35.337399999999995	37.0	37.0	37.0	32.2	37.0
105-109	35.3253	37.0	37.0	37.0	32.2	37.0
110-114	35.2084	37.0	37.0	37.0	29.8	37.0
115-119	35.2344	37.0	37.0	37.0	34.6	37.0
120-124	35.1743	37.0	37.0	37.0	27.4	37.0
125-129	35.2074	37.0	37.0	37.0	27.4	37.0
130-134	34.8303	37.0	37.0	37.0	25.0	37.0
135-139	34.983399999999996	37.0	37.0	37.0	25.0	37.0
140-144	34.935199999999995	37.0	37.0	37.0	25.0	37.0
145-149	34.737700000000004	37.0	37.0	37.0	25.0	37.0
150	34.8235	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	7.0
22	8.0
23	11.0
24	13.0
25	19.0
26	10.0
27	15.0
28	16.0
29	37.0
30	43.0
31	51.0
32	59.0
33	116.0
34	248.0
35	822.0
36	2410.0
37	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.35	10.625	5.949999999999999	25.074999999999996
2	20.45	20.575	35.475	23.5
3	19.6	28.475	24.525	27.400000000000002
4	8.35	11.1	41.9	38.65
5	10.7	39.825	37.625	11.85
6	32.65	42.8	15.925	8.625
7	29.2	30.15	22.325	18.325
8	28.65	32.775	19.15	19.425
9	25.900000000000002	11.125	13.125	49.85
10-14	23.18	23.13	31.330000000000002	22.36
15-19	23.195	28.439999999999998	24.995	23.369999999999997
20-24	24.709999999999997	25.355	25.2	24.735
25-29	23.135	25.014999999999997	26.179999999999996	25.669999999999998
30-34	24.765	26.1	25.145	23.990000000000002
35-39	23.395	26.355	25.88	24.37
40-44	24.525	26.77	24.535	24.169999999999998
45-49	23.93	24.884999999999998	25.94	25.245
50-54	24.11	25.155	26.284999999999997	24.45
55-59	24.255	25.525	25.81	24.41
60-64	24.005000000000003	25.569999999999997	25.885	24.54
65-69	24.92	25.074999999999996	25.525	24.48
70-74	23.3	25.685000000000002	26.005	25.009999999999998
75-79	24.57	26.529999999999998	24.834999999999997	24.065
80-84	23.935000000000002	25.180000000000003	25.240000000000002	25.645
85-89	23.990000000000002	24.525	25.535000000000004	25.95
90-94	24.865000000000002	25.39	25.75	23.995
95-99	24.44	25.655	26.365	23.54
100-104	24.805	25.14	26.06	23.995
105-109	23.01	24.595	26.515	25.88
110-114	24.654999999999998	25.27	25.009999999999998	25.064999999999998
115-119	24.154999999999998	24.495	26.995	24.355
120-124	23.94	25.465	26.055	24.54
125-129	24.18	25.185000000000002	26.505000000000003	24.13
130-134	24.905	24.22	26.26	24.615000000000002
135-139	24.83	24.7	26.1	24.37
140-144	24.709999999999997	23.82	26.340000000000003	25.130000000000003
145-149	24.195	23.9	26.295	25.61
150	26.224999999999998	24.725	25.25	23.799999999999997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.0
11	1.0
12	1.0
13	1.0
14	1.0
15	0.0
16	0.0
17	0.0
18	0.5
19	1.5
20	2.5
21	3.0
22	2.0
23	3.5
24	5.0
25	4.5
26	17.0
27	29.0
28	28.5
29	27.0
30	24.0
31	20.5
32	24.0
33	47.5
34	65.5
35	65.0
36	71.5
37	81.0
38	88.5
39	82.5
40	98.0
41	120.5
42	99.5
43	86.0
44	92.0
45	91.0
46	99.0
47	107.5
48	117.0
49	162.0
50	151.5
51	129.0
52	181.5
53	214.5
54	214.0
55	202.0
56	194.5
57	185.0
58	153.5
59	147.0
60	134.0
61	85.5
62	54.0
63	48.0
64	43.5
65	33.0
66	22.0
67	10.0
68	4.0
69	5.0
70	4.5
71	0.5
72	1.0
73	2.0
74	2.5
75	2.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.77499999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18586088939567	53.400000000000006
2	10.376282782212087	13.65
3	2.66058532877233	5.25
4	1.444317749904979	3.8
5	1.1402508551881414	3.75
6	0.7221588749524895	2.85
7	0.7221588749524895	3.325
8	0.3040668947168377	1.6
9	0.41809198023565186	2.475
>10	1.0262257696693273	9.9
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	39	0.975	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	26	0.65	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	20	0.5	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	18	0.44999999999999996	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	17	0.42500000000000004	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	17	0.42500000000000004	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	17	0.42500000000000004	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	17	0.42500000000000004	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	15	0.375	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	14	0.35000000000000003	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	14	0.35000000000000003	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	14	0.35000000000000003	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	14	0.35000000000000003	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	13	0.325	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	13	0.325	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	12	0.3	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	12	0.3	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	11	0.27499999999999997	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	11	0.27499999999999997	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	11	0.27499999999999997	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	11	0.27499999999999997	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	10	0.25	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	10	0.25	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	10	0.25	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	10	0.25	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	10	0.25	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	10	0.25	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	9	0.22499999999999998	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	9	0.22499999999999998	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	9	0.22499999999999998	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	9	0.22499999999999998	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	9	0.22499999999999998	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	9	0.22499999999999998	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	9	0.22499999999999998	No Hit
GTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCCCTACAATCACCACG	9	0.22499999999999998	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	9	0.22499999999999998	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	9	0.22499999999999998	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	9	0.22499999999999998	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	8	0.2	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	8	0.2	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	8	0.2	No Hit
GCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGC	8	0.2	No Hit
GCCTAACACTTCCATCTTCCCTGTTTCCAACTCATGCCTTCAAACGTACA	8	0.2	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	8	0.2	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	8	0.2	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	8	0.2	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC	7	0.17500000000000002	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	7	0.17500000000000002	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	7	0.17500000000000002	No Hit
GACCATCTCAGTATGTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCC	7	0.17500000000000002	No Hit
GTACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	7	0.17500000000000002	No Hit
GAGAGACACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAG	7	0.17500000000000002	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	7	0.17500000000000002	No Hit
GTCCCAGTATATTCGGAACTGCGGAGTCTGACCGCGGTGTGCTTCTACTG	7	0.17500000000000002	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	7	0.17500000000000002	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	7	0.17500000000000002	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	7	0.17500000000000002	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	7	0.17500000000000002	No Hit
GCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGT	7	0.17500000000000002	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	7	0.17500000000000002	No Hit
GGGTCTTTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGAT	7	0.17500000000000002	No Hit
GGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAG	7	0.17500000000000002	No Hit
GTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAGCATCCACA	7	0.17500000000000002	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	7	0.17500000000000002	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	7	0.17500000000000002	No Hit
GTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGGTTACCTAAAT	6	0.15	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	6	0.15	No Hit
GTACATGGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCT	6	0.15	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	6	0.15	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	6	0.15	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	6	0.15	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	6	0.15	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	6	0.15	No Hit
CTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGC	6	0.15	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	6	0.15	No Hit
GTACATGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCTC	6	0.15	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	6	0.15	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	6	0.15	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	6	0.15	No Hit
GGATTACTCGGCAGGTCCCAGGAGCCAGTTCCGCATCCCCTGCTGTCCCG	6	0.15	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	6	0.15	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	6	0.15	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	6	0.15	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	6	0.15	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	5	0.125	No Hit
CTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTGTCCATCTGC	5	0.125	No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG	5	0.125	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	5	0.125	No Hit
GGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACATGAGTAG	5	0.125	No Hit
CTCTACAACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACC	5	0.125	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	5	0.125	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	5	0.125	No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG	5	0.125	No Hit
GTATTTAGCTGACTTGCAACCCTACACGGAGGTAATATTAAATGATCTCC	5	0.125	No Hit
CCCATGAGCAGAGCCCCTACAATCACCACGACAACGAGGACCACCACCAC	5	0.125	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	5	0.125	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	5	0.125	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	5	0.125	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	5	0.125	No Hit
GGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATG	5	0.125	No Hit
GAGTCAACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGCATGCAGATCTT	5	0.125	No Hit
GCTTAGAGGTGGGTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTA	5	0.125	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	5	0.125	No Hit
GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG	5	0.125	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	5	0.125	No Hit
CGCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGG	5	0.125	No Hit
GGGTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTT	5	0.125	No Hit
GACACATTACAACATCACCCCACATCTACAGACACCAAAGCCTCCAACCT	5	0.125	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	5	0.125	No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT	5	0.125	No Hit
CCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGG	5	0.125	No Hit
AGATTGAGCAGTAAAAAGAATTAGAACTCTAAACTTAAGCTAATAGAGTA	5	0.125	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	5	0.125	No Hit
GGCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.025	0.0
38-39	0.0	0.0	0.0	0.0625	0.0
40-41	0.0	0.0	0.0	0.1125	0.0
42-43	0.0	0.0	0.0	0.25	0.0
44-45	0.0	0.0	0.0	0.2875	0.0
46-47	0.0	0.0	0.0	0.42500000000000004	0.0
48-49	0.0	0.0	0.0	0.525	0.0
50-51	0.0	0.0	0.0	0.5874999999999999	0.0
52-53	0.0	0.0	0.0	0.6875	0.0
54-55	0.0	0.0	0.0	0.7625	0.0
56-57	0.0	0.0	0.0	0.9624999999999999	0.0
58-59	0.0	0.0	0.0	1.0875	0.0
60-61	0.0	0.0	0.0	1.225	0.0
62-63	0.0	0.0	0.0	1.2999999999999998	0.0
64-65	0.0	0.0	0.0	1.4125	0.0
66-67	0.0	0.0	0.0	1.6	0.0
68-69	0.0	0.0	0.0	1.7374999999999998	0.0
70-71	0.0	0.0	0.0	1.8875	0.0
72-73	0.0	0.0	0.0	2.0	0.0
74-75	0.0	0.0	0.0	2.1125	0.0
76-77	0.0	0.0	0.0	2.325	0.0
78-79	0.0	0.0	0.0	2.4125	0.0
80-81	0.0	0.0	0.0	2.6375	0.0
82-83	0.0	0.0	0.0	2.9000000000000004	0.0
84-85	0.0	0.0	0.0	3.0375	0.0
86-87	0.0	0.0	0.0	3.2125	0.0
88-89	0.0	0.0	0.0	3.3625	0.0
90-91	0.0	0.0	0.0	3.5875000000000004	0.0
92-93	0.0	0.0	0.0	3.825	0.0
94-95	0.0	0.0	0.0	4.075	0.0
96-97	0.0	0.0	0.0	4.2875	0.0
98-99	0.0	0.0	0.0	4.5	0.0
100-101	0.0	0.0	0.0	4.762499999999999	0.0
102-103	0.0	0.0	0.0	5.112500000000001	0.0
104-105	0.0	0.0	0.0	5.3375	0.0
106-107	0.0	0.0	0.0	5.8375	0.0
108-109	0.0	0.0	0.0	6.137499999999999	0.0
110-111	0.0	0.0	0.0	6.3	0.0
112-113	0.0	0.0	0.0	6.6875	0.0
114-115	0.0	0.0	0.0	7.2125	0.0
116-117	0.0	0.0	0.0	7.6	0.0
118-119	0.0	0.0	0.0	7.9625	0.0
120-121	0.0	0.0	0.0	8.2875	0.0
122-123	0.0	0.0	0.0	8.6875	0.0
124-125	0.0	0.0	0.0	9.149999999999999	0.0
126-127	0.0	0.0	0.0	9.4875	0.0
128-129	0.0	0.0	0.0	9.875	0.0
130-131	0.0	0.0	0.0	10.45	0.0
132-133	0.0	0.0	0.0	10.975	0.0
134-135	0.0	0.0	0.0	11.4875	0.0
136-137	0.0	0.0	0.0	12.037500000000001	0.0
138	0.0	0.0	0.0	12.275	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGGA	10	0.006973645	144.0	5
TACATGG	15	1.1730364E-4	144.0	2
TGGGGAG	10	0.006973645	144.0	6
CATGGGG	10	0.006973645	144.0	4
GGGGAGG	10	0.006973645	144.0	7
ACATGGG	25	8.956223E-4	86.399994	3
GTACATG	25	8.956223E-4	86.399994	1
GGGGGGG	30	0.0015031899	23.999998	140-144
>>END_MODULE
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
Read 3224069 spots for SRR31990351.sra
Written 3224069 spots for SRR31990351.sra
SRR ids: ['SRR31990351.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_4n8una5s
SRR31990351.sra spots: 25792552
blocks: [[1, 3224069], [3224070, 6448138], [6448139, 9672207], [9672208, 12896276], [12896277, 16120345], [16120346, 19344414], [19344415, 22568483], [22568484, 25792552]]
SRR31990351 file size 9471640
SRR31990351 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990351 SRR31990351_1.fastq SRR31990351_2.fastq
Input file:	SRR31990351_1.fastq
Paired file:	SRR31990351_2.fastq
trimmed:	SRR31990351-trimmed-pair1.fastq, SRR31990351-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:19:07 2026 >> started

Sat Apr 25 17:19:45 2026 >> done (37.316s)
25792552 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
25792552 (100.00%) read pairs available; of these:
    2027 ( 0.01%) trimmed read pairs available after processing
25790525 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
142	       2	  0.00%
143	       3	  0.00%
144	       0	  0.00%
145	       6	  0.00%
146	       3	  0.00%
147	      56	  0.00%
148	     101	  0.00%
149	    1856	  0.01%
150	25790525	 99.99%
25792552 reads passed initial QC


criterion=sequence-density
sequence-density=11.19
sequence-density-rank=1
fanout-score=36.64
fanout-score-rank=2
prefix-density=12.22
prefix-fanout=33.5
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=23
fanout-score=244.75
fanout-score-rank=1
prefix-density=2.93
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=11.25
sequence-density-rank=1
fanout-score=35.92
fanout-score-rank=2
prefix-density=12.29
prefix-fanout=32.9
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=22
fanout-score=279.80
fanout-score-rank=1
prefix-density=2.89
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG -y CTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA -o SRR31990351 SRR31990351_1.fastq SRR31990351_2.fastq
Input file:	SRR31990351_1.fastq
Paired file:	SRR31990351_2.fastq
trimmed:	SRR31990351-trimmed-pair1.fastq, SRR31990351-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:21:32 2026 >> started

Sat Apr 25 17:22:13 2026 >> done (41.752s)
21493793 read pairs processed; of these:
      14 ( 0.00%) short read pairs filtered out after trimming by size control
       5 ( 0.00%) empty read pairs filtered out after trimming by size control
21493774 (100.00%) read pairs available; of these:
 3374420 (15.70%) trimmed read pairs available after processing
18119354 (84.30%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       4	  0.00%
 19	       6	  0.00%
 20	       1	  0.00%
 21	      14	  0.00%
 22	      15	  0.00%
 23	      30	  0.00%
 24	      22	  0.00%
 25	      50	  0.00%
 26	      43	  0.00%
 27	     114	  0.00%
 28	     324	  0.00%
 29	     431	  0.00%
 30	     482	  0.00%
 31	     882	  0.00%
 32	     851	  0.00%
 33	    1350	  0.01%
 34	    1221	  0.01%
 35	    1811	  0.01%
 36	    3196	  0.01%
 37	    4438	  0.02%
 38	    5511	  0.03%
 39	    5399	  0.03%
 40	    6255	  0.03%
 41	    5906	  0.03%
 42	    6398	  0.03%
 43	    6282	  0.03%
 44	    9375	  0.04%
 45	    7781	  0.04%
 46	   10295	  0.05%
 47	    9508	  0.04%
 48	   11170	  0.05%
 49	    9431	  0.04%
 50	    9809	  0.05%
 51	    8861	  0.04%
 52	    8651	  0.04%
 53	    9356	  0.04%
 54	    9119	  0.04%
 55	   14763	  0.07%
 56	   12007	  0.06%
 57	   10786	  0.05%
 58	   10058	  0.05%
 59	    9335	  0.04%
 60	   11309	  0.05%
 61	   10489	  0.05%
 62	   12409	  0.06%
 63	   13865	  0.06%
 64	   10844	  0.05%
 65	   10591	  0.05%
 66	   15831	  0.07%
 67	   14440	  0.07%
 68	   12898	  0.06%
 69	   18428	  0.09%
 70	   14889	  0.07%
 71	   17743	  0.08%
 72	   15351	  0.07%
 73	   15041	  0.07%
 74	   15697	  0.07%
 75	   17200	  0.08%
 76	   16543	  0.08%
 77	   13838	  0.06%
 78	   14445	  0.07%
 79	   14784	  0.07%
 80	   21237	  0.10%
 81	   30279	  0.14%
 82	   25606	  0.12%
 83	   14872	  0.07%
 84	   16298	  0.08%
 85	   17757	  0.08%
 86	   20642	  0.10%
 87	   23992	  0.11%
 88	   20920	  0.10%
 89	   24183	  0.11%
 90	   21651	  0.10%
 91	   19845	  0.09%
 92	   21356	  0.10%
 93	   25949	  0.12%
 94	   23709	  0.11%
 95	   29728	  0.14%
 96	   26475	  0.12%
 97	   22786	  0.11%
 98	   31656	  0.15%
 99	   32039	  0.15%
100	   27931	  0.13%
101	   28537	  0.13%
102	   26423	  0.12%
103	   32094	  0.15%
104	   33678	  0.16%
105	   37877	  0.18%
106	   35571	  0.17%
107	   37493	  0.17%
108	   37620	  0.18%
109	   34294	  0.16%
110	   32093	  0.15%
111	   36454	  0.17%
112	   38415	  0.18%
113	   37701	  0.18%
114	   38071	  0.18%
115	   32091	  0.15%
116	   31678	  0.15%
117	   34554	  0.16%
118	   32537	  0.15%
119	   42828	  0.20%
120	   40867	  0.19%
121	   43969	  0.20%
122	   44838	  0.21%
123	   48435	  0.23%
124	   45035	  0.21%
125	   46755	  0.22%
126	   48195	  0.22%
127	   48082	  0.22%
128	   39081	  0.18%
129	   53565	  0.25%
130	   58907	  0.27%
131	   51581	  0.24%
132	   55820	  0.26%
133	   60485	  0.28%
134	   53458	  0.25%
135	   64734	  0.30%
136	   60119	  0.28%
137	   48259	  0.22%
138	   56324	  0.26%
139	   59659	  0.28%
140	   69343	  0.32%
141	   65040	  0.30%
142	   71804	  0.33%
143	   61952	  0.29%
144	   69270	  0.32%
145	   54883	  0.26%
146	   65490	  0.30%
147	   71815	  0.33%
148	   77035	  0.36%
149	   84414	  0.39%
150	18117664	 84.29%


criterion=sequence-density
sequence-density=1.77
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=29
prefix-density=1.80
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=30
fanout-score=238.39
fanout-score-rank=1
prefix-density=2.93
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.77
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=27
prefix-density=1.78
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=30
fanout-score=248.95
fanout-score-rank=1
prefix-density=2.90
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990351 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 17:23:47
                             Started mapping on |	Apr 25 17:23:47
                                    Finished on |	Apr 25 17:26:41
       Mapping speed, Million of reads per hour |	533.64

                          Number of input reads |	25792533
                      Average input read length |	286
                                    UNIQUE READS:
                   Uniquely mapped reads number |	19773344
                        Uniquely mapped reads % |	76.66%
                          Average mapped length |	288.22
                       Number of splices: Total |	19054031
            Number of splices: Annotated (sjdb) |	18945090
                       Number of splices: GT/AG |	18975639
                       Number of splices: GC/AG |	29053
                       Number of splices: AT/AC |	4307
               Number of splices: Non-canonical |	45032
                      Mismatch rate per base, % |	0.31%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.56
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.41
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2488632
             % of reads mapped to multiple loci |	9.65%
        Number of reads mapped to too many loci |	43729
             % of reads mapped to too many loci |	0.17%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.42%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3530557	3530557	3530557
N_multimapping	2488632	2488632	2488632
N_noFeature	1879666	10145667	10084390
N_ambiguous	1878934	247251	246271
UnstrandedReadsAssigned:16014744 PositiveStrandReadsAssigned:9380426 NegativeStrandReadsAssigned:9442683
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR31990351 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990351-trimmed-pair1.fastq
                             SRR31990351-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 25,792,533 reads, 20,698,306 reads pseudoaligned
[quant] estimated average fragment length: 227.134
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,112 rounds

 109283 SRR31990351.ke.tsv
  52636 SRR31990351.se.tsv
 161919 total
==> SRR31990351.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	11.5	0	0
ENSMUST00000196221.1	9	4.5	0	0
ENSMUST00000179664.1	11	6.5	0	0
ENSMUST00000178537.1	12	7.5	0	0
ENSMUST00000178862.1	14	9.5	0	0
ENSMUST00000179520.1	11	6.5	0	0
ENSMUST00000179883.1	16	11.5	0	0
ENSMUST00000195858.1	10	5.5	0	0
ENSMUST00000179932.1	12	7.5	0	0

==> SRR31990351.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	1
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990351 completed mapping pipeline successfully
