Starting /dee2/code/volunteer_pipeline.sh SRR31990348
    current disk space = 42277840
    free memory = 197885956 
SRR31990348 SRAfilesize
2d02a9e0008a601f6ecd937f0594577e  SRR31990348.sra
SRR31990348.sra file validated
SRR31990348 is paired end
SRR31990348 is conventional basespace
SRR31990348 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990348_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.4065	37.0	37.0	37.0	25.0	37.0
2	32.3515	37.0	25.0	37.0	11.0	37.0
3	33.8985	37.0	37.0	37.0	25.0	37.0
4	34.081	37.0	37.0	37.0	25.0	37.0
5	34.851	37.0	37.0	37.0	25.0	37.0
6	34.7745	37.0	37.0	37.0	25.0	37.0
7	34.9255	37.0	37.0	37.0	25.0	37.0
8	35.371	37.0	37.0	37.0	25.0	37.0
9	35.5905	37.0	37.0	37.0	37.0	37.0
10-14	35.25749999999999	37.0	37.0	37.0	27.4	37.0
15-19	35.276399999999995	37.0	37.0	37.0	32.2	37.0
20-24	35.341499999999996	37.0	37.0	37.0	32.2	37.0
25-29	34.8531	37.0	37.0	37.0	27.4	37.0
30-34	35.038	37.0	37.0	37.0	25.0	37.0
35-39	35.266999999999996	37.0	37.0	37.0	25.0	37.0
40-44	34.9214	37.0	37.0	37.0	25.0	37.0
45-49	34.9577	37.0	37.0	37.0	25.0	37.0
50-54	34.7462	37.0	37.0	37.0	25.0	37.0
55-59	34.816199999999995	37.0	37.0	37.0	25.0	37.0
60-64	34.7817	37.0	37.0	37.0	25.0	37.0
65-69	34.503	37.0	37.0	37.0	25.0	37.0
70-74	34.3695	37.0	37.0	37.0	25.0	37.0
75-79	34.5334	37.0	37.0	37.0	25.0	37.0
80-84	34.344699999999996	37.0	37.0	37.0	25.0	37.0
85-89	34.3087	37.0	37.0	37.0	25.0	37.0
90-94	34.2378	37.0	37.0	37.0	25.0	37.0
95-99	34.134	37.0	37.0	37.0	25.0	37.0
100-104	33.9744	37.0	37.0	37.0	25.0	37.0
105-109	33.75580000000001	37.0	37.0	37.0	22.2	37.0
110-114	33.644	37.0	37.0	37.0	25.0	37.0
115-119	33.6132	37.0	37.0	37.0	22.2	37.0
120-124	33.3352	37.0	37.0	37.0	22.2	37.0
125-129	33.345299999999995	37.0	37.0	37.0	22.2	37.0
130-134	33.2295	37.0	37.0	37.0	19.4	37.0
135-139	32.9872	37.0	37.0	37.0	13.8	37.0
140-144	32.8378	37.0	37.0	37.0	13.8	37.0
145-149	32.174	37.0	32.2	37.0	11.0	37.0
150	32.5675	37.0	37.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	6.0
22	10.0
23	10.0
24	10.0
25	10.0
26	12.0
27	23.0
28	52.0
29	61.0
30	91.0
31	188.0
32	263.0
33	495.0
34	915.0
35	1339.0
36	512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.95	11.450000000000001	5.075	24.525
2	20.869125821121777	19.580596260737746	36.558868115209705	22.991409802930775
3	19.575	29.675	23.775	26.974999999999998
4	8.425	10.25	42.65	38.675
5	9.875	41.3	38.3	10.525
6	32.625	42.8	15.8	8.774999999999999
7	28.050000000000004	31.424999999999997	21.775	18.75
8	27.975	34.849999999999994	19.775000000000002	17.4
9	27.450000000000003	11.075	14.025000000000002	47.449999999999996
10-14	22.785	23.465	31.535000000000004	22.215
15-19	23.200000000000003	29.585	24.635	22.58
20-24	24.765	25.569999999999997	25.435000000000002	24.23
25-29	23.18	25.835	26.0	24.985
30-34	24.72	26.795	25.46	23.025000000000002
35-39	23.724999999999998	26.72	26.055	23.5
40-44	24.285	27.58	24.72	23.415
45-49	24.79	25.490000000000002	26.0	23.72
50-54	24.5	25.255	26.91	23.335
55-59	24.515	25.645	26.005	23.835
60-64	23.86	26.505000000000003	25.765	23.87
65-69	24.69	25.650000000000002	25.805	23.855
70-74	23.380000000000003	25.629999999999995	26.840000000000003	24.15
75-79	23.735	26.619999999999997	25.779999999999998	23.865
80-84	24.05	25.755	25.715	24.48
85-89	24.245	24.740000000000002	25.535000000000004	25.480000000000004
90-94	24.01	25.86	26.3	23.830000000000002
95-99	23.735	26.279999999999998	25.874999999999996	24.11
100-104	23.89	25.36	26.584999999999997	24.165
105-109	23.14	25.455	25.97	25.435000000000002
110-114	24.64	25.44	24.425	25.495
115-119	23.995	24.915000000000003	26.674999999999997	24.415
120-124	24.060000000000002	25.759999999999998	25.655	24.525
125-129	25.074999999999996	25.19	26.240000000000002	23.494999999999997
130-134	25.215	24.355	25.480000000000004	24.95
135-139	25.25	24.36	25.650000000000002	24.740000000000002
140-144	25.64	23.735	26.145000000000003	24.48
145-149	25.115	24.355	25.650000000000002	24.88
150	24.85	26.075	24.25	24.825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	0.0
17	0.5
18	0.5
19	1.0
20	1.0
21	0.5
22	1.5
23	5.0
24	7.0
25	6.5
26	17.5
27	30.5
28	31.5
29	25.5
30	23.0
31	21.0
32	26.0
33	42.0
34	60.5
35	79.5
36	88.5
37	87.0
38	95.0
39	107.0
40	128.5
41	145.0
42	127.0
43	104.5
44	113.5
45	120.0
46	113.0
47	110.0
48	103.0
49	124.0
50	135.0
51	131.0
52	170.0
53	198.5
54	195.5
55	187.0
56	175.5
57	155.5
58	128.5
59	130.5
60	112.5
61	74.5
62	57.0
63	54.5
64	50.5
65	37.5
66	22.5
67	8.0
68	8.0
69	7.5
70	3.5
71	2.0
72	2.0
73	1.5
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.10000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.71806167400881	55.65
2	10.022026431718063	13.65
3	3.1938325991189425	6.525
4	1.57856093979442	4.3
5	0.9177679882525698	3.125
6	0.6975036710719531	2.85
7	0.47723935389133626	2.275
8	0.3303964757709251	1.7999999999999998
9	0.18355359765051393	1.125
>10	0.881057268722467	8.7
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	29	0.7250000000000001	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	27	0.675	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	20	0.5	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	18	0.44999999999999996	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	17	0.42500000000000004	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	17	0.42500000000000004	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	17	0.42500000000000004	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	15	0.375	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	14	0.35000000000000003	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	14	0.35000000000000003	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	14	0.35000000000000003	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	13	0.325	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	13	0.325	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	12	0.3	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	12	0.3	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	12	0.3	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	11	0.27499999999999997	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	11	0.27499999999999997	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	11	0.27499999999999997	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	11	0.27499999999999997	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	10	0.25	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	10	0.25	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	10	0.25	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	10	0.25	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	9	0.22499999999999998	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	9	0.22499999999999998	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	9	0.22499999999999998	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	9	0.22499999999999998	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	9	0.22499999999999998	No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC	8	0.2	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	8	0.2	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	8	0.2	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	8	0.2	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	8	0.2	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	8	0.2	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	8	0.2	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	8	0.2	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	8	0.2	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	7	0.17500000000000002	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	7	0.17500000000000002	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	7	0.17500000000000002	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	7	0.17500000000000002	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	7	0.17500000000000002	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	7	0.17500000000000002	No Hit
GCCTAAGGCTCATAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTA	7	0.17500000000000002	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	7	0.17500000000000002	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	7	0.17500000000000002	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	7	0.17500000000000002	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	7	0.17500000000000002	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	7	0.17500000000000002	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	7	0.17500000000000002	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	6	0.15	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	6	0.15	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	6	0.15	No Hit
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT	6	0.15	No Hit
GTACATGGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCT	6	0.15	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	6	0.15	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	6	0.15	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	6	0.15	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	6	0.15	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	6	0.15	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	6	0.15	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	6	0.15	No Hit
CCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCC	6	0.15	No Hit
ATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCC	6	0.15	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	6	0.15	No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA	6	0.15	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	6	0.15	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	6	0.15	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	6	0.15	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	5	0.125	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	5	0.125	No Hit
CTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCAT	5	0.125	No Hit
GATTTACACTGAAGGACCTGTCAAAGGCTTCCTTGGTAACTTCCAGGAAG	5	0.125	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	5	0.125	No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA	5	0.125	No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG	5	0.125	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	5	0.125	No Hit
CTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTG	5	0.125	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	5	0.125	No Hit
GTACATGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCTC	5	0.125	No Hit
GCCGAGGAGACACAGGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTG	5	0.125	No Hit
TTTCTAGACTCCTTAAACTCTGGGACATTCAGTTTACAAATGATAGAATG	5	0.125	No Hit
GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT	5	0.125	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	5	0.125	No Hit
CTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGACATCTGC	5	0.125	No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT	5	0.125	No Hit
GTTCTAATTCTTTTTACTGCTCAATCTTTTTAATTAAAAACTTATCTGCG	5	0.125	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	5	0.125	No Hit
CTCTGATTCCATGAGGAGGGCCTCAAGGACTTGAAGAAATCCTGGGCAGA	5	0.125	No Hit
GGATTGGACGGGACACTGTGAAGGCCCTGCACGCCTCAGGAGCCAAAGTG	5	0.125	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	5	0.125	No Hit
GAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTC	5	0.125	No Hit
GGCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTC	5	0.125	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.037500000000000006	0.0
38-39	0.0	0.0	0.0	0.0625	0.0
40-41	0.0	0.0	0.0	0.21250000000000002	0.0
42-43	0.0	0.0	0.0	0.225	0.0
44-45	0.0	0.0	0.0	0.3375	0.0
46-47	0.0	0.0	0.0	0.4	0.0
48-49	0.0	0.0	0.0	0.475	0.0
50-51	0.0	0.0	0.0	0.525	0.0
52-53	0.0	0.0	0.0	0.575	0.0
54-55	0.0	0.0	0.0	0.675	0.0
56-57	0.0	0.0	0.0	0.725	0.0
58-59	0.0	0.0	0.0	0.7875	0.0
60-61	0.0	0.0	0.0	1.0125	0.0
62-63	0.0	0.0	0.0	1.1124999999999998	0.0
64-65	0.0	0.0	0.0	1.1875	0.0
66-67	0.0	0.0	0.0	1.4125	0.0
68-69	0.0	0.0	0.0	1.525	0.0
70-71	0.0	0.0	0.0	1.65	0.0
72-73	0.0	0.0	0.0	1.75	0.0
74-75	0.0	0.0	0.0	1.8125	0.0
76-77	0.0	0.0	0.0	2.0125	0.0
78-79	0.0	0.0	0.0	2.1375	0.0
80-81	0.0	0.0	0.0	2.3	0.0
82-83	0.0	0.0	0.0	2.7	0.0
84-85	0.0	0.0	0.0	2.7875	0.0
86-87	0.0	0.0	0.0	2.9875	0.0
88-89	0.0	0.0	0.0	3.325	0.0
90-91	0.0	0.0	0.0	3.55	0.0
92-93	0.0	0.0	0.0	3.725	0.0
94-95	0.0	0.0	0.0	3.95	0.0
96-97	0.0	0.0	0.0	4.25	0.0
98-99	0.0	0.0	0.0	4.425000000000001	0.0
100-101	0.0	0.0	0.0	4.675	0.0
102-103	0.0	0.0	0.0	4.975	0.0
104-105	0.0	0.0	0.0	5.1	0.0
106-107	0.0	0.0	0.0	5.324999999999999	0.0
108-109	0.0	0.0	0.0	5.6	0.0
110-111	0.0	0.0	0.0	6.025	0.0
112-113	0.0	0.0	0.0	6.3875	0.0
114-115	0.0	0.0	0.0	6.75	0.0
116-117	0.0	0.0	0.0	6.975	0.0
118-119	0.0	0.0	0.0	7.35	0.0
120-121	0.0	0.0	0.0	7.775	0.0
122-123	0.0	0.0	0.0	8.2625	0.0
124-125	0.0	0.0	0.0	8.9	0.0
126-127	0.0	0.0	0.0	9.25	0.0
128-129	0.0	0.0	0.0	9.787500000000001	0.0
130-131	0.0	0.0	0.0	10.175	0.0
132-133	0.0	0.0	0.0	10.6875	0.0
134-135	0.0	0.0	0.0	11.462499999999999	0.0
136-137	0.0	0.0	0.0	12.175	0.0
138	0.0	0.0	0.0	12.6	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCTT	10	0.006973645	144.0	6
GAAACCT	10	0.006973645	144.0	5
AACCTTA	10	0.006973645	144.0	7
ACCTTAC	10	0.006973645	144.0	8
CCTTACA	10	0.006973645	144.0	9
AATACTC	10	0.006973645	144.0	6
ATACTCA	10	0.006973645	144.0	7
TTTTTTT	220	0.009731082	6.5454545	20-24
>>END_MODULE
SRR31990348 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990348_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.098	37.0	37.0	37.0	37.0	37.0
2	36.588	37.0	37.0	37.0	37.0	37.0
3	36.351	37.0	37.0	37.0	37.0	37.0
4	36.532	37.0	37.0	37.0	37.0	37.0
5	36.546	37.0	37.0	37.0	37.0	37.0
6	36.54	37.0	37.0	37.0	37.0	37.0
7	36.578	37.0	37.0	37.0	37.0	37.0
8	36.697	37.0	37.0	37.0	37.0	37.0
9	36.6845	37.0	37.0	37.0	37.0	37.0
10-14	36.5719	37.0	37.0	37.0	37.0	37.0
15-19	36.4876	37.0	37.0	37.0	37.0	37.0
20-24	36.4564	37.0	37.0	37.0	37.0	37.0
25-29	36.4979	37.0	37.0	37.0	37.0	37.0
30-34	36.4097	37.0	37.0	37.0	37.0	37.0
35-39	36.45890000000001	37.0	37.0	37.0	37.0	37.0
40-44	36.2911	37.0	37.0	37.0	37.0	37.0
45-49	36.261199999999995	37.0	37.0	37.0	37.0	37.0
50-54	36.2384	37.0	37.0	37.0	37.0	37.0
55-59	36.194	37.0	37.0	37.0	37.0	37.0
60-64	36.305099999999996	37.0	37.0	37.0	37.0	37.0
65-69	36.2153	37.0	37.0	37.0	37.0	37.0
70-74	36.0546	37.0	37.0	37.0	37.0	37.0
75-79	36.125699999999995	37.0	37.0	37.0	37.0	37.0
80-84	35.9691	37.0	37.0	37.0	37.0	37.0
85-89	35.986700000000006	37.0	37.0	37.0	37.0	37.0
90-94	35.9062	37.0	37.0	37.0	37.0	37.0
95-99	35.8565	37.0	37.0	37.0	37.0	37.0
100-104	35.8129	37.0	37.0	37.0	37.0	37.0
105-109	35.8676	37.0	37.0	37.0	37.0	37.0
110-114	35.7113	37.0	37.0	37.0	37.0	37.0
115-119	35.7526	37.0	37.0	37.0	37.0	37.0
120-124	35.599399999999996	37.0	37.0	37.0	37.0	37.0
125-129	35.694599999999994	37.0	37.0	37.0	37.0	37.0
130-134	35.3193	37.0	37.0	37.0	32.2	37.0
135-139	35.5433	37.0	37.0	37.0	34.6	37.0
140-144	35.4755	37.0	37.0	37.0	37.0	37.0
145-149	35.281	37.0	37.0	37.0	32.2	37.0
150	35.212	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	5.0
22	12.0
23	8.0
24	9.0
25	6.0
26	5.0
27	3.0
28	5.0
29	5.0
30	13.0
31	16.0
32	26.0
33	58.0
34	170.0
35	676.0
36	2836.0
37	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	59.575	9.85	5.5	25.074999999999996
2	20.825	19.825	37.475	21.875
3	19.2	28.65	25.525	26.625
4	8.1	10.975	42.5	38.425
5	9.475	39.6	38.725	12.2
6	32.675	42.725	15.85	8.75
7	27.575	30.5	23.150000000000002	18.775
8	27.950000000000003	33.324999999999996	19.6	19.125
9	26.05	11.65	13.700000000000001	48.6
10-14	22.585	22.939999999999998	32.1	22.375
15-19	23.06	28.9	24.915000000000003	23.125
20-24	24.965	25.6	25.245	24.19
25-29	22.48	25.895000000000003	26.75	24.875
30-34	23.919999999999998	26.575	25.924999999999997	23.580000000000002
35-39	23.64	26.465	26.265	23.630000000000003
40-44	24.240000000000002	27.325	25.215	23.22
45-49	24.525	26.284999999999997	25.64	23.549999999999997
50-54	23.57	25.535000000000004	26.950000000000003	23.945
55-59	23.16	25.96	26.6	24.279999999999998
60-64	24.275	26.815	25.490000000000002	23.419999999999998
65-69	24.08	26.02	25.735000000000003	24.165
70-74	24.375	25.115	26.11	24.4
75-79	23.655	26.905	25.39	24.05
80-84	24.52	26.05	25.335	24.095
85-89	24.075	24.775	25.745	25.405
90-94	23.91	25.564999999999998	26.775	23.75
95-99	23.405	25.72	27.084999999999997	23.79
100-104	24.535	24.985	26.415	24.065
105-109	23.380000000000003	25.365	26.015	25.240000000000002
110-114	24.535	25.72	25.180000000000003	24.565
115-119	23.62	24.675	27.534999999999997	24.169999999999998
120-124	23.75	26.040000000000003	25.775	24.435000000000002
125-129	24.315	24.975	26.97	23.74
130-134	23.955000000000002	24.51	27.05	24.485
135-139	23.830000000000002	25.014999999999997	26.334999999999997	24.82
140-144	24.495	24.69	26.900000000000002	23.915
145-149	24.77	24.03	26.3	24.9
150	24.575	27.0	25.424999999999997	23.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.5
8	1.0
9	0.5
10	0.5
11	1.0
12	1.0
13	1.0
14	0.5
15	0.5
16	0.5
17	1.0
18	1.5
19	1.0
20	1.5
21	1.5
22	1.5
23	2.5
24	3.0
25	5.5
26	16.0
27	26.5
28	30.0
29	25.0
30	19.5
31	22.5
32	33.0
33	55.0
34	72.5
35	76.0
36	95.5
37	96.5
38	90.0
39	95.5
40	122.0
41	147.0
42	115.5
43	92.0
44	113.5
45	119.0
46	120.0
47	115.0
48	97.5
49	128.5
50	136.0
51	131.0
52	176.5
53	217.0
54	211.0
55	181.5
56	157.5
57	144.5
58	133.0
59	131.0
60	115.5
61	79.0
62	55.0
63	45.5
64	38.5
65	39.0
66	29.5
67	11.0
68	6.0
69	2.5
70	1.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.5
77	0.5
78	0.5
79	1.0
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.64999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04098994586234	51.1
2	11.48491879350348	14.85
3	3.7509667440061873	7.2749999999999995
4	1.740139211136891	4.5
5	1.160092807424594	3.75
6	0.7733952049497294	3.0
7	0.19334880123743234	0.8750000000000001
8	0.42536736272235115	2.1999999999999997
9	0.34802784222737815	2.025
>10	1.082753286929621	10.424999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	41	1.0250000000000001	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	24	0.6	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	21	0.525	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	20	0.5	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	19	0.475	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	17	0.42500000000000004	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	17	0.42500000000000004	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	16	0.4	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	15	0.375	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	14	0.35000000000000003	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	14	0.35000000000000003	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	13	0.325	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	13	0.325	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	13	0.325	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	13	0.325	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	13	0.325	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	12	0.3	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	12	0.3	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	12	0.3	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	12	0.3	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	11	0.27499999999999997	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	11	0.27499999999999997	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	11	0.27499999999999997	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	11	0.27499999999999997	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	11	0.27499999999999997	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	11	0.27499999999999997	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	10	0.25	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	10	0.25	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	9	0.22499999999999998	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	9	0.22499999999999998	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	9	0.22499999999999998	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	9	0.22499999999999998	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	9	0.22499999999999998	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	9	0.22499999999999998	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	9	0.22499999999999998	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	9	0.22499999999999998	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	9	0.22499999999999998	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	8	0.2	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	8	0.2	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	8	0.2	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	8	0.2	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	8	0.2	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	8	0.2	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	8	0.2	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	8	0.2	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	8	0.2	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	8	0.2	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	8	0.2	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	7	0.17500000000000002	No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC	7	0.17500000000000002	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	7	0.17500000000000002	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	7	0.17500000000000002	No Hit
GGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCA	7	0.17500000000000002	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	6	0.15	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	6	0.15	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	6	0.15	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	6	0.15	No Hit
GATTTACACTGAAGGACCTGTCAAAGGCTTCCTTGGTAACTTCCAGGAAG	6	0.15	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	6	0.15	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	6	0.15	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	6	0.15	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	6	0.15	No Hit
CCTTTAATCCTAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCG	6	0.15	No Hit
ATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTC	6	0.15	No Hit
GTCCATCTGCTGCAGCTCAGCTTCTTCGGACATCTGCCCAGGATTTCTTC	6	0.15	No Hit
GTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGACATCTGCCC	6	0.15	No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT	6	0.15	No Hit
GTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCCCTACAATCACCACG	6	0.15	No Hit
GATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTCTCTTGTGGGAGGG	6	0.15	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	6	0.15	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	6	0.15	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	6	0.15	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	6	0.15	No Hit
CATCTGCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAG	5	0.125	No Hit
CTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTGTCCATCTGC	5	0.125	No Hit
CTACAAACACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGC	5	0.125	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	5	0.125	No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG	5	0.125	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	5	0.125	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	5	0.125	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	5	0.125	No Hit
GCTTTGTGCAGTCCCAGCACCCAGTTAGAACAGCCTCTGTGGTGGGCCGG	5	0.125	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	5	0.125	No Hit
GAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCAT	5	0.125	No Hit
GTTATATACTTTAATATTATGCTTTGAGAATTAAGGCCATAGTGCACACA	5	0.125	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	5	0.125	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	5	0.125	No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC	5	0.125	No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA	5	0.125	No Hit
GTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAG	5	0.125	No Hit
CTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGC	5	0.125	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	5	0.125	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	5	0.125	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	5	0.125	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	5	0.125	No Hit
GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT	5	0.125	No Hit
GTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAGCATCCACA	5	0.125	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	5	0.125	No Hit
CCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGG	5	0.125	No Hit
GAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAACGGT	5	0.125	No Hit
GGATCCCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACC	5	0.125	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	5	0.125	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.037500000000000006	0.0
38-39	0.0	0.0	0.0	0.0625	0.0
40-41	0.0	0.0	0.0	0.21250000000000002	0.0
42-43	0.0	0.0	0.0	0.225	0.0
44-45	0.0	0.0	0.0	0.36250000000000004	0.0
46-47	0.0	0.0	0.0	0.42500000000000004	0.0
48-49	0.0	0.0	0.0	0.5	0.0
50-51	0.0	0.0	0.0	0.55	0.0
52-53	0.0	0.0	0.0	0.6	0.0
54-55	0.0	0.0	0.0	0.7	0.0
56-57	0.0	0.0	0.0	0.75	0.0
58-59	0.0	0.0	0.0	0.825	0.0
60-61	0.0	0.0	0.0	1.0625	0.0
62-63	0.0	0.0	0.0	1.1625	0.0
64-65	0.0	0.0	0.0	1.2374999999999998	0.0
66-67	0.0	0.0	0.0	1.4625	0.0
68-69	0.0	0.0	0.0	1.6124999999999998	0.0
70-71	0.0	0.0	0.0	1.75	0.0
72-73	0.0	0.0	0.0	1.85	0.0
74-75	0.0	0.0	0.0	1.9375	0.0
76-77	0.0	0.0	0.0	2.1375	0.0
78-79	0.0	0.0	0.0	2.2875	0.0
80-81	0.0	0.0	0.0	2.45	0.0
82-83	0.0	0.0	0.0	2.85	0.0
84-85	0.0	0.0	0.0	2.9375	0.0
86-87	0.0	0.0	0.0	3.1375	0.0
88-89	0.0	0.0	0.0	3.5	0.0
90-91	0.0	0.0	0.0	3.7625	0.0
92-93	0.0	0.0	0.0	3.975	0.0
94-95	0.0	0.0	0.0	4.225	0.0
96-97	0.0	0.0	0.0	4.6125	0.0
98-99	0.0	0.0	0.0	4.825	0.0
100-101	0.0	0.0	0.0	5.1	0.0
102-103	0.0	0.0	0.0	5.4	0.0
104-105	0.0	0.0	0.0	5.5625	0.0
106-107	0.0	0.0	0.0	5.875	0.0
108-109	0.0	0.0	0.0	6.199999999999999	0.0
110-111	0.0	0.0	0.0	6.6625	0.0
112-113	0.0	0.0	0.0	7.0625	0.0
114-115	0.0	0.0	0.0	7.55	0.0
116-117	0.0	0.0	0.0	7.8	0.0
118-119	0.0	0.0	0.0	8.2125	0.0
120-121	0.0	0.0	0.0	8.6875	0.0
122-123	0.0	0.0	0.0	9.2625	0.0
124-125	0.0	0.0	0.0	9.95	0.0
126-127	0.0	0.0	0.0	10.4	0.0
128-129	0.0	0.0	0.0	11.0375	0.0
130-131	0.0	0.0	0.0	11.55	0.0
132-133	0.0	0.0	0.0	12.1375	0.0
134-135	0.0	0.0	0.0	12.962499999999999	0.0
136-137	0.0	0.0	0.0	13.725	0.0
138	0.0	0.0	0.0	14.225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	10	0.006973645	144.0	3
ACACCTT	10	0.006973645	144.0	6
TACATGG	10	0.006973645	144.0	2
CATGGGG	10	0.006973645	144.0	4
GGGGGGG	180	0.001368918	8.0	140-144
>>END_MODULE
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790445 spots for SRR31990348.sra
Written 2790445 spots for SRR31990348.sra
Read 2790451 spots for SRR31990348.sra
Written 2790451 spots for SRR31990348.sra
SRR ids: ['SRR31990348.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_tnvwv_fg
SRR31990348.sra spots: 22323566
blocks: [[1, 2790445], [2790446, 5580890], [5580891, 8371335], [8371336, 11161780], [11161781, 13952225], [13952226, 16742670], [16742671, 19533115], [19533116, 22323566]]
SRR31990348 file size 8196276
SRR31990348 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990348 SRR31990348_1.fastq SRR31990348_2.fastq
Input file:	SRR31990348_1.fastq
Paired file:	SRR31990348_2.fastq
trimmed:	SRR31990348-trimmed-pair1.fastq, SRR31990348-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:41:41 2026 >> started

Sat Apr 25 17:42:16 2026 >> done (35.146s)
22323566 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
22323566 (100.00%) read pairs available; of these:
    1741 ( 0.01%) trimmed read pairs available after processing
22321825 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
127	       2	  0.00%
128	       0	  0.00%
129	       0	  0.00%
130	       0	  0.00%
131	       0	  0.00%
132	       0	  0.00%
133	       0	  0.00%
134	       0	  0.00%
135	       0	  0.00%
136	       0	  0.00%
137	       0	  0.00%
138	       0	  0.00%
139	       0	  0.00%
140	       0	  0.00%
141	       0	  0.00%
142	       0	  0.00%
143	       0	  0.00%
144	       1	  0.00%
145	       3	  0.00%
146	       2	  0.00%
147	      19	  0.00%
148	     114	  0.00%
149	    1600	  0.01%
150	22321825	 99.99%
22323566 reads passed initial QC


criterion=sequence-density
sequence-density=12.20
sequence-density-rank=1
fanout-score=36.28
fanout-score-rank=2
prefix-density=13.41
prefix-fanout=33.0
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=21
fanout-score=124.87
fanout-score-rank=1
prefix-density=2.87
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=13.38
sequence-density-rank=1
fanout-score=35.22
fanout-score-rank=2
prefix-density=14.54
prefix-fanout=32.4
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=19
fanout-score=225.61
fanout-score-rank=1
prefix-density=2.94
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA -o SRR31990348 SRR31990348_1.fastq SRR31990348_2.fastq
Input file:	SRR31990348_1.fastq
Paired file:	SRR31990348_2.fastq
trimmed:	SRR31990348-trimmed-pair1.fastq, SRR31990348-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:44:15 2026 >> started

Sat Apr 25 17:45:02 2026 >> done (47.004s)
19134485 read pairs processed; of these:
      13 ( 0.00%) short read pairs filtered out after trimming by size control
      10 ( 0.00%) empty read pairs filtered out after trimming by size control
19134462 (100.00%) read pairs available; of these:
 3466466 (18.12%) trimmed read pairs available after processing
15667996 (81.88%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       2	  0.00%
 19	       6	  0.00%
 20	       5	  0.00%
 21	      20	  0.00%
 22	      12	  0.00%
 23	      22	  0.00%
 24	      19	  0.00%
 25	      47	  0.00%
 26	      46	  0.00%
 27	     170	  0.00%
 28	     419	  0.00%
 29	     549	  0.00%
 30	     733	  0.00%
 31	    1259	  0.01%
 32	    1210	  0.01%
 33	    1926	  0.01%
 34	    1504	  0.01%
 35	    2358	  0.01%
 36	    4181	  0.02%
 37	    5824	  0.03%
 38	    6775	  0.04%
 39	    6707	  0.04%
 40	    7446	  0.04%
 41	    6858	  0.04%
 42	    7065	  0.04%
 43	    7303	  0.04%
 44	   10757	  0.06%
 45	    9237	  0.05%
 46	   11393	  0.06%
 47	   10569	  0.06%
 48	   12184	  0.06%
 49	   10103	  0.05%
 50	   11444	  0.06%
 51	   10232	  0.05%
 52	   10172	  0.05%
 53	   10133	  0.05%
 54	   10418	  0.05%
 55	   16349	  0.09%
 56	   12942	  0.07%
 57	   12236	  0.06%
 58	   11415	  0.06%
 59	   10683	  0.06%
 60	   12311	  0.06%
 61	   11186	  0.06%
 62	   13108	  0.07%
 63	   15750	  0.08%
 64	   11805	  0.06%
 65	   12011	  0.06%
 66	   17036	  0.09%
 67	   15395	  0.08%
 68	   13579	  0.07%
 69	   19329	  0.10%
 70	   16371	  0.09%
 71	   18557	  0.10%
 72	   16514	  0.09%
 73	   15986	  0.08%
 74	   16615	  0.09%
 75	   19311	  0.10%
 76	   17586	  0.09%
 77	   14360	  0.08%
 78	   15841	  0.08%
 79	   15106	  0.08%
 80	   22052	  0.12%
 81	   30524	  0.16%
 82	   25094	  0.13%
 83	   16239	  0.08%
 84	   16945	  0.09%
 85	   19337	  0.10%
 86	   22481	  0.12%
 87	   24021	  0.13%
 88	   22798	  0.12%
 89	   24695	  0.13%
 90	   22416	  0.12%
 91	   20522	  0.11%
 92	   22535	  0.12%
 93	   28691	  0.15%
 94	   24931	  0.13%
 95	   31367	  0.16%
 96	   29518	  0.15%
 97	   23471	  0.12%
 98	   33215	  0.17%
 99	   30593	  0.16%
100	   28310	  0.15%
101	   30579	  0.16%
102	   26792	  0.14%
103	   33791	  0.18%
104	   34321	  0.18%
105	   37641	  0.20%
106	   34878	  0.18%
107	   35315	  0.18%
108	   40657	  0.21%
109	   37697	  0.20%
110	   32861	  0.17%
111	   37032	  0.19%
112	   37933	  0.20%
113	   37200	  0.19%
114	   38608	  0.20%
115	   33199	  0.17%
116	   34718	  0.18%
117	   36510	  0.19%
118	   32974	  0.17%
119	   41746	  0.22%
120	   42264	  0.22%
121	   44081	  0.23%
122	   44526	  0.23%
123	   52639	  0.28%
124	   44171	  0.23%
125	   46641	  0.24%
126	   49920	  0.26%
127	   48287	  0.25%
128	   40581	  0.21%
129	   55169	  0.29%
130	   64210	  0.34%
131	   49461	  0.26%
132	   53235	  0.28%
133	   65222	  0.34%
134	   54738	  0.29%
135	   64760	  0.34%
136	   59738	  0.31%
137	   47016	  0.25%
138	   60036	  0.31%
139	   58652	  0.31%
140	   66256	  0.35%
141	   63892	  0.33%
142	   66794	  0.35%
143	   63959	  0.33%
144	   67338	  0.35%
145	   52525	  0.27%
146	   61937	  0.32%
147	   67680	  0.35%
148	   84591	  0.44%
149	   82946	  0.43%
150	15666500	 81.88%


criterion=sequence-density
sequence-density=1.37
sequence-density-rank=1
fanout-score=2.17
fanout-score-rank=28
prefix-density=1.44
prefix-fanout=2.1
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=32
fanout-score=120.37
fanout-score-rank=1
prefix-density=2.86
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.51
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=31
prefix-density=1.52
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=234.69
fanout-score-rank=1
prefix-density=2.94
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990348 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 17:46:35
                             Started mapping on |	Apr 25 17:46:35
                                    Finished on |	Apr 25 17:49:51
       Mapping speed, Million of reads per hour |	410.02

                          Number of input reads |	22323543
                      Average input read length |	284
                                    UNIQUE READS:
                   Uniquely mapped reads number |	16164921
                        Uniquely mapped reads % |	72.41%
                          Average mapped length |	287.13
                       Number of splices: Total |	15511466
            Number of splices: Annotated (sjdb) |	15429736
                       Number of splices: GT/AG |	15454767
                       Number of splices: GC/AG |	25096
                       Number of splices: AT/AC |	3783
               Number of splices: Non-canonical |	27820
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.55
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.27
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2818556
             % of reads mapped to multiple loci |	12.63%
        Number of reads mapped to too many loci |	33429
             % of reads mapped to too many loci |	0.15%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	14.74%
                     % of reads unmapped: other |	0.07%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3340066	3340066	3340066
N_multimapping	2818556	2818556	2818556
N_noFeature	1345832	8131698	8215617
N_ambiguous	1617838	246340	240731
UnstrandedReadsAssigned:13201251 PositiveStrandReadsAssigned:7786883 NegativeStrandReadsAssigned:7708573
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR31990348 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990348-trimmed-pair1.fastq
                             SRR31990348-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,323,543 reads, 18,124,275 reads pseudoaligned
[quant] estimated average fragment length: 219.228
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 993 rounds

 109283 SRR31990348.ke.tsv
  52636 SRR31990348.se.tsv
 161919 total
==> SRR31990348.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	2	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	2	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR31990348.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	1
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990348 completed mapping pipeline successfully
