Starting /dee2/code/volunteer_pipeline.sh SRR31990345
    current disk space = 63231056
    free memory = 192349372 
SRR31990345 SRAfilesize
e918bf9301de69bd828f7e97ec6f4256  SRR31990345.sra
SRR31990345.sra file validated
SRR31990345 is paired end
SRR31990345 is conventional basespace
SRR31990345 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.998	37.0	37.0	37.0	37.0	37.0
2	36.1815	37.0	37.0	37.0	37.0	37.0
3	36.319	37.0	37.0	37.0	37.0	37.0
4	36.3285	37.0	37.0	37.0	37.0	37.0
5	36.329	37.0	37.0	37.0	37.0	37.0
6	36.415	37.0	37.0	37.0	37.0	37.0
7	36.36	37.0	37.0	37.0	37.0	37.0
8	36.322	37.0	37.0	37.0	37.0	37.0
9	36.254	37.0	37.0	37.0	37.0	37.0
10-14	36.30159999999999	37.0	37.0	37.0	37.0	37.0
15-19	36.2971	37.0	37.0	37.0	37.0	37.0
20-24	36.282500000000006	37.0	37.0	37.0	37.0	37.0
25-29	36.216499999999996	37.0	37.0	37.0	37.0	37.0
30-34	36.22525	37.0	37.0	37.0	37.0	37.0
35-39	36.0644	37.0	37.0	37.0	37.0	37.0
40-44	35.946	37.0	37.0	37.0	37.0	37.0
45-49	35.833800000000004	37.0	37.0	37.0	37.0	37.0
50-54	35.7299	37.0	37.0	37.0	37.0	37.0
55-59	35.7599	37.0	37.0	37.0	37.0	37.0
60-64	35.7742	37.0	37.0	37.0	37.0	37.0
65-69	35.7321	37.0	37.0	37.0	37.0	37.0
70-74	35.715700000000005	37.0	37.0	37.0	37.0	37.0
75-79	35.682500000000005	37.0	37.0	37.0	37.0	37.0
80-84	35.633900000000004	37.0	37.0	37.0	37.0	37.0
85-89	35.4936	37.0	37.0	37.0	37.0	37.0
90-94	35.423700000000004	37.0	37.0	37.0	37.0	37.0
95-99	35.451499999999996	37.0	37.0	37.0	37.0	37.0
100-104	35.3236	37.0	37.0	37.0	37.0	37.0
105-109	35.169	37.0	37.0	37.0	29.8	37.0
110-114	35.1755	37.0	37.0	37.0	29.8	37.0
115-119	35.1408	37.0	37.0	37.0	29.8	37.0
120-124	35.05	37.0	37.0	37.0	25.0	37.0
125-129	34.825900000000004	37.0	37.0	37.0	25.0	37.0
130-134	34.7954	37.0	37.0	37.0	25.0	37.0
135-139	34.72580000000001	37.0	37.0	37.0	25.0	37.0
140-144	34.4988	37.0	37.0	37.0	25.0	37.0
145-149	34.463800000000006	37.0	37.0	37.0	25.0	37.0
150	34.4215	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	6.0
22	12.0
23	16.0
24	12.0
25	11.0
26	13.0
27	16.0
28	29.0
29	37.0
30	44.0
31	86.0
32	118.0
33	180.0
34	209.0
35	494.0
36	2531.0
37	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	60.47094188376754	10.095190380761524	4.909819639278557	24.524048096192384
2	19.8	19.35	37.0	23.849999999999998
3	18.5	29.525000000000002	24.474999999999998	27.500000000000004
4	8.575000000000001	11.35	40.825	39.25
5	9.700000000000001	42.075	36.85	11.375
6	35.425000000000004	39.975	16.55	8.05
7	28.675	31.55	21.7	18.075
8	29.099999999999998	34.275	18.35	18.275
9	27.775	11.425	13.125	47.675
10-14	22.93	22.845	31.35	22.875
15-19	23.94	28.625	24.57	22.865
20-24	24.91	25.53	25.53	24.03
25-29	23.022302230223023	25.317531753175317	26.112611261126112	25.54755475547555
30-34	23.84119205960298	26.3863193159658	25.661283064153206	24.111205560278016
35-39	23.425	26.365	26.625	23.585
40-44	24.605	27.169999999999998	24.465	23.76
45-49	24.490000000000002	25.305	25.585	24.62
50-54	23.51	26.0	26.064999999999998	24.425
55-59	24.279999999999998	25.929999999999996	25.595000000000002	24.195
60-64	24.25	26.025	25.4	24.325
65-69	24.39	25.72	25.955000000000002	23.935000000000002
70-74	23.745	25.564999999999998	26.735	23.955000000000002
75-79	24.45	26.490000000000002	24.529999999999998	24.529999999999998
80-84	24.335	26.215	24.73	24.72
85-89	23.845	25.655	25.41	25.09
90-94	24.065	26.795	25.650000000000002	23.49
95-99	24.035	26.32	25.929999999999996	23.715
100-104	24.65	25.314999999999998	26.055	23.98
105-109	23.215	25.305	26.450000000000003	25.03
110-114	24.36	25.91	24.455	25.275
115-119	24.060000000000002	24.834999999999997	26.619999999999997	24.485
120-124	23.74	25.825	26.009999999999998	24.425
125-129	24.775	24.785	26.27	24.169999999999998
130-134	24.23	24.22	26.724999999999998	24.825
135-139	24.875	25.71	24.995	24.42
140-144	24.555	24.8	26.395000000000003	24.25
145-149	24.235	24.32	26.27	25.174999999999997
150	25.474999999999998	26.625	25.05	22.85
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	1.5
10	1.5
11	1.5
12	2.0
13	2.5
14	2.0
15	0.5
16	1.0
17	1.0
18	1.0
19	2.5
20	2.5
21	1.5
22	3.0
23	7.0
24	12.0
25	15.0
26	20.5
27	22.5
28	29.5
29	27.5
30	21.0
31	20.5
32	28.5
33	57.0
34	68.5
35	64.5
36	79.0
37	80.0
38	80.0
39	87.0
40	89.0
41	115.0
42	107.0
43	75.5
44	93.5
45	105.0
46	102.0
47	115.0
48	112.0
49	143.0
50	143.0
51	132.5
52	183.0
53	216.5
54	232.5
55	217.5
56	185.0
57	152.0
58	149.5
59	157.5
60	128.0
61	91.5
62	56.0
63	38.0
64	34.0
65	30.5
66	22.5
67	11.5
68	5.5
69	2.5
70	1.0
71	0.0
72	1.5
73	2.5
74	1.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	1.5
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.01
30-34	0.005
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75728155339806	50.7
2	12.116504854368932	15.6
3	3.5339805825242716	6.825
4	1.5922330097087378	4.1000000000000005
5	1.0485436893203883	3.375
6	0.6601941747572815	2.55
7	0.34951456310679613	1.575
8	0.31067961165048547	1.6
9	0.3883495145631068	2.25
>10	1.2427184466019419	11.425
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	29	0.7250000000000001	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	24	0.6	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	23	0.575	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	21	0.525	No Hit
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	21	0.525	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	19	0.475	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	16	0.4	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	16	0.4	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	16	0.4	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	16	0.4	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	15	0.375	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	15	0.375	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	15	0.375	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	13	0.325	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	13	0.325	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	13	0.325	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	13	0.325	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	12	0.3	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	12	0.3	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	11	0.27499999999999997	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	11	0.27499999999999997	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	11	0.27499999999999997	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	11	0.27499999999999997	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	11	0.27499999999999997	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	10	0.25	No Hit
CCCATGAGCAGAGCCCCTACAATCACCACGACAACGAGGACCACCACCAC	10	0.25	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	10	0.25	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	10	0.25	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	10	0.25	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	10	0.25	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	10	0.25	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	10	0.25	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	9	0.22499999999999998	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	9	0.22499999999999998	No Hit
GTACATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	9	0.22499999999999998	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	9	0.22499999999999998	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	9	0.22499999999999998	No Hit
GCTTAGAGGTGGGTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTA	9	0.22499999999999998	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	9	0.22499999999999998	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	9	0.22499999999999998	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	9	0.22499999999999998	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	9	0.22499999999999998	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	8	0.2	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	8	0.2	No Hit
CTACAAGGCCGACCTTCAAACTAGAACCTTTTAGAACTTCACAAAACCTC	8	0.2	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	8	0.2	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	8	0.2	No Hit
GTACATGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACAT	8	0.2	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	8	0.2	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	8	0.2	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	7	0.17500000000000002	No Hit
GTGTTGTACTTCTTTGTAATGCTTGCGATGGCCTCGTTCTCCTCAGGATT	7	0.17500000000000002	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	7	0.17500000000000002	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	7	0.17500000000000002	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	7	0.17500000000000002	No Hit
CACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGAT	7	0.17500000000000002	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	7	0.17500000000000002	No Hit
ACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACCTTCGTTT	7	0.17500000000000002	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	7	0.17500000000000002	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	6	0.15	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	6	0.15	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	6	0.15	No Hit
GTACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	6	0.15	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	6	0.15	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	6	0.15	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	6	0.15	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	6	0.15	No Hit
GGTCTTTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGATA	6	0.15	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	6	0.15	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	6	0.15	No Hit
GTATTAGGGCTAAAATTAGGATAGATAAGATTAAGGCTAGGACACCTCCT	6	0.15	No Hit
GGTCCTGATGGAGAGTCCACCGGATTACTCGGCAGGTCCCAGGAGCCAGT	6	0.15	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	6	0.15	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	6	0.15	No Hit
GGCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTC	6	0.15	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	6	0.15	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	5	0.125	No Hit
GACCATCTCAGTATGTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCC	5	0.125	No Hit
TTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGATATAGTA	5	0.125	No Hit
GACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCTGCTTGTCTGACAAAG	5	0.125	No Hit
GTACATGGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCT	5	0.125	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	5	0.125	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	5	0.125	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	5	0.125	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	5	0.125	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	5	0.125	No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA	5	0.125	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	5	0.125	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	5	0.125	No Hit
GCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGC	5	0.125	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	5	0.125	No Hit
CCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGCTGAAGAGACTGGTGG	5	0.125	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	5	0.125	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	5	0.125	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	5	0.125	No Hit
CCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGT	5	0.125	No Hit
GTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCCCTACAATCACCACG	5	0.125	No Hit
GGATCCCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACC	5	0.125	No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA	5	0.125	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	5	0.125	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	5	0.125	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	5	0.125	No Hit
GGTCAGCACGGTGCTCACAGAGGCAAGGAATTTATCCAGAGAGGCATGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0125	0.0
34-35	0.0	0.0	0.0	0.025	0.0
36-37	0.0	0.0	0.0	0.025	0.0
38-39	0.0	0.0	0.0	0.0625	0.0
40-41	0.0	0.0	0.0	0.1	0.0
42-43	0.0	0.0	0.0	0.15	0.0
44-45	0.0	0.0	0.0	0.25	0.0
46-47	0.0	0.0	0.0	0.275	0.0
48-49	0.0	0.0	0.0	0.2875	0.0
50-51	0.0	0.0	0.0	0.42500000000000004	0.0
52-53	0.0	0.0	0.0	0.575	0.0
54-55	0.0	0.0	0.0	0.6375	0.0
56-57	0.0	0.0	0.0	0.8	0.0
58-59	0.0	0.0	0.0	0.9375	0.0
60-61	0.0	0.0	0.0	0.9875	0.0
62-63	0.0	0.0	0.0	1.0499999999999998	0.0
64-65	0.0	0.0	0.0	1.075	0.0
66-67	0.0	0.0	0.0	1.1625	0.0
68-69	0.0	0.0	0.0	1.225	0.0
70-71	0.0	0.0	0.0	1.375	0.0
72-73	0.0	0.0	0.0	1.5125	0.0
74-75	0.0	0.0	0.0	1.65	0.0
76-77	0.0	0.0	0.0	1.8624999999999998	0.0
78-79	0.0	0.0	0.0	1.975	0.0
80-81	0.0	0.0	0.0	2.0875000000000004	0.0
82-83	0.0	0.0	0.0	2.275	0.0
84-85	0.0	0.0	0.0	2.4125	0.0
86-87	0.0	0.0	0.0	2.7125	0.0
88-89	0.0	0.0	0.0	2.925	0.0
90-91	0.0	0.0	0.0	3.15	0.0
92-93	0.0	0.0	0.0	3.2874999999999996	0.0
94-95	0.0	0.0	0.0	3.325	0.0
96-97	0.0	0.0	0.0	3.575	0.0
98-99	0.0	0.0	0.0	3.9	0.0
100-101	0.0	0.0	0.0	4.075	0.0
102-103	0.0	0.0	0.0	4.3625	0.0
104-105	0.0	0.0	0.0	4.5875	0.0
106-107	0.0	0.0	0.0	4.975	0.0
108-109	0.0	0.0	0.0	5.3	0.0
110-111	0.0	0.0	0.0	5.5875	0.0
112-113	0.0	0.0	0.0	5.9125	0.0
114-115	0.0	0.0	0.0	6.3375	0.0
116-117	0.0	0.0	0.0	6.449999999999999	0.0
118-119	0.0	0.0	0.0	6.55	0.0
120-121	0.0	0.0	0.0	6.775	0.0
122-123	0.0	0.0	0.0	7.0125	0.0
124-125	0.0	0.0	0.0	7.3125	0.0
126-127	0.0	0.0	0.0	7.625	0.0
128-129	0.0	0.0	0.0	7.925	0.0
130-131	0.0	0.0	0.0	8.3125	0.0
132-133	0.0	0.0	0.0	8.7875	0.0
134-135	0.0	0.0	0.0	9.05	0.0
136-137	0.0	0.0	0.0	9.375	0.0
138	0.0	0.0	0.0	9.7	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCCCA	10	0.006973645	144.0	9
ACTGTAT	10	0.006973645	144.0	9
TATACAG	10	0.006973645	144.0	5
GTACTGT	10	0.006973645	144.0	7
TACTGTA	10	0.006973645	144.0	8
GTCCAAA	10	0.006973645	144.0	1
GGGGGGG	185	1.5685127E-6	10.118919	140-144
>>END_MODULE
SRR31990345 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990345_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.4935	37.0	37.0	37.0	25.0	37.0
2	35.8705	37.0	37.0	37.0	37.0	37.0
3	35.9675	37.0	37.0	37.0	37.0	37.0
4	35.9255	37.0	37.0	37.0	37.0	37.0
5	35.995	37.0	37.0	37.0	37.0	37.0
6	35.979	37.0	37.0	37.0	37.0	37.0
7	36.0555	37.0	37.0	37.0	37.0	37.0
8	36.0535	37.0	37.0	37.0	37.0	37.0
9	36.0095	37.0	37.0	37.0	37.0	37.0
10-14	36.014799999999994	37.0	37.0	37.0	37.0	37.0
15-19	36.00339999999999	37.0	37.0	37.0	37.0	37.0
20-24	35.9859	37.0	37.0	37.0	37.0	37.0
25-29	36.0486	37.0	37.0	37.0	37.0	37.0
30-34	35.8949	37.0	37.0	37.0	37.0	37.0
35-39	35.7923	37.0	37.0	37.0	37.0	37.0
40-44	35.718999999999994	37.0	37.0	37.0	37.0	37.0
45-49	35.5178	37.0	37.0	37.0	37.0	37.0
50-54	35.5488	37.0	37.0	37.0	37.0	37.0
55-59	35.4034	37.0	37.0	37.0	37.0	37.0
60-64	35.5163	37.0	37.0	37.0	37.0	37.0
65-69	35.348	37.0	37.0	37.0	37.0	37.0
70-74	35.4011	37.0	37.0	37.0	37.0	37.0
75-79	35.3345	37.0	37.0	37.0	37.0	37.0
80-84	35.299899999999994	37.0	37.0	37.0	37.0	37.0
85-89	35.131800000000005	37.0	37.0	37.0	29.8	37.0
90-94	35.1212	37.0	37.0	37.0	27.4	37.0
95-99	35.124	37.0	37.0	37.0	29.8	37.0
100-104	34.95270000000001	37.0	37.0	37.0	25.0	37.0
105-109	34.9828	37.0	37.0	37.0	25.0	37.0
110-114	34.8521	37.0	37.0	37.0	25.0	37.0
115-119	34.8943	37.0	37.0	37.0	25.0	37.0
120-124	34.7312	37.0	37.0	37.0	25.0	37.0
125-129	34.6158	37.0	37.0	37.0	25.0	37.0
130-134	34.552	37.0	37.0	37.0	25.0	37.0
135-139	34.541399999999996	37.0	37.0	37.0	25.0	37.0
140-144	34.3809	37.0	37.0	37.0	25.0	37.0
145-149	34.3959	37.0	37.0	37.0	25.0	37.0
150	34.2905	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	5.0
20	4.0
21	14.0
22	19.0
23	22.0
24	22.0
25	17.0
26	17.0
27	24.0
28	27.0
29	45.0
30	48.0
31	71.0
32	85.0
33	137.0
34	295.0
35	788.0
36	2183.0
37	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	59.925	10.375	5.55	24.15
2	19.225	18.825	38.25	23.7
3	18.875	29.775000000000002	24.125	27.224999999999998
4	8.075000000000001	11.200000000000001	42.15	38.574999999999996
5	10.125	41.475	37.55	10.85
6	33.900000000000006	41.3	16.775000000000002	8.025
7	29.599999999999998	30.7	21.675	18.025
8	28.449999999999996	32.824999999999996	20.175	18.55
9	28.749999999999996	10.75	13.750000000000002	46.75
10-14	22.16	23.46	32.275	22.105
15-19	23.095	28.27	25.169999999999998	23.465
20-24	24.23	25.56	26.165	24.044999999999998
25-29	23.02	24.990000000000002	26.605	25.385
30-34	24.14	26.82	25.655	23.385
35-39	24.065	26.015	26.76	23.16
40-44	23.925	26.540000000000003	26.08	23.455000000000002
45-49	24.815	25.155	26.384999999999998	23.645
50-54	23.44	25.480000000000004	26.490000000000002	24.59
55-59	23.830000000000002	25.55	26.674999999999997	23.945
60-64	24.185000000000002	26.33	25.825	23.66
65-69	24.3	26.055	25.619999999999997	24.025
70-74	24.34	25.055	26.625	23.98
75-79	24.224999999999998	26.179999999999996	25.34	24.255
80-84	24.58	26.619999999999997	24.57	24.23
85-89	24.455	24.94	25.255	25.35
90-94	23.835	25.97	25.724999999999998	24.47
95-99	24.085	25.855	25.924999999999997	24.135
100-104	24.32	24.83	27.16	23.69
105-109	22.91	26.21	25.369999999999997	25.509999999999998
110-114	24.495	25.775	24.62	25.11
115-119	23.82	25.019999999999996	26.779999999999998	24.38
120-124	23.64	25.935000000000002	25.91	24.515
125-129	24.224999999999998	25.21	26.979999999999997	23.585
130-134	24.84	24.295	25.995	24.87
135-139	24.345	25.074999999999996	25.624999999999996	24.955
140-144	23.46	25.525	27.015	24.0
145-149	23.935000000000002	25.305	26.229999999999997	24.529999999999998
150	24.349999999999998	26.3	26.25	23.1
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	1.0
13	1.5
14	0.5
15	1.0
16	1.5
17	1.0
18	1.5
19	1.0
20	1.5
21	5.5
22	6.5
23	8.0
24	8.0
25	9.0
26	25.5
27	39.5
28	42.0
29	40.5
30	31.5
31	20.0
32	26.0
33	48.5
34	58.0
35	61.0
36	70.0
37	76.0
38	85.5
39	88.5
40	97.0
41	117.5
42	116.5
43	95.5
44	98.0
45	98.5
46	91.0
47	87.5
48	100.0
49	146.5
50	145.5
51	146.5
52	199.5
53	235.0
54	234.5
55	185.5
56	160.0
57	160.5
58	148.5
59	147.0
60	121.0
61	88.5
62	59.0
63	39.0
64	40.5
65	37.5
66	18.5
67	6.0
68	5.5
69	2.5
70	2.0
71	3.5
72	2.0
73	0.5
74	0.5
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70465732677016	52.625
2	11.624384702764104	15.35
3	3.0670200681560016	6.075
4	2.1204089360090874	5.6000000000000005
5	1.0602044680045437	3.5000000000000004
6	0.37864445285876563	1.5
7	0.34078000757288907	1.575
8	0.15145778114350625	0.8
9	0.34078000757288907	2.025
>10	1.21166224914805	10.95
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	30	0.75	No Hit
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	29	0.7250000000000001	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	21	0.525	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	20	0.5	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	19	0.475	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	16	0.4	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	15	0.375	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	15	0.375	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	14	0.35000000000000003	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	14	0.35000000000000003	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	14	0.35000000000000003	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	14	0.35000000000000003	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	13	0.325	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	12	0.3	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	12	0.3	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	12	0.3	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	12	0.3	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	12	0.3	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	11	0.27499999999999997	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	11	0.27499999999999997	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	11	0.27499999999999997	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	11	0.27499999999999997	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	10	0.25	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	10	0.25	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	10	0.25	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	10	0.25	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	10	0.25	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	10	0.25	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	10	0.25	No Hit
CCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGGTCCTCGTTGTCGTGG	10	0.25	No Hit
GTACATGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACAT	10	0.25	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	10	0.25	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	9	0.22499999999999998	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	9	0.22499999999999998	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	9	0.22499999999999998	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	9	0.22499999999999998	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	9	0.22499999999999998	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	9	0.22499999999999998	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	9	0.22499999999999998	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	9	0.22499999999999998	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	9	0.22499999999999998	No Hit
CCCATGAGCAGAGCCCCTACAATCACCACGACAACGAGGACCACCACCAC	8	0.2	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	8	0.2	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	8	0.2	No Hit
GTCTCAGAGTTAGTGTGAATGAAGGAACTTAAGAAAGATCAGGACAGGCA	8	0.2	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	7	0.17500000000000002	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	7	0.17500000000000002	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	7	0.17500000000000002	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	7	0.17500000000000002	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	7	0.17500000000000002	No Hit
CTCTGATTCCATGAGGAGGGCCTCAAGGACTTGAAGAAATCCTGGGCAGA	7	0.17500000000000002	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	7	0.17500000000000002	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	7	0.17500000000000002	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	7	0.17500000000000002	No Hit
GGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGTAATTACGGCTCCA	6	0.15	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	6	0.15	No Hit
GTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAGGAAAGCCAGG	6	0.15	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	6	0.15	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	6	0.15	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	6	0.15	No Hit
GCTTAGAGGTGGGTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTA	6	0.15	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	6	0.15	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	6	0.15	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	6	0.15	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC	5	0.125	No Hit
GTACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5	0.125	No Hit
CTACAAACACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGC	5	0.125	No Hit
GGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAA	5	0.125	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	5	0.125	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	5	0.125	No Hit
GACCATCTCAGTATGTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCC	5	0.125	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	5	0.125	No Hit
CTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTACTTAGTCTTA	5	0.125	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	5	0.125	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	5	0.125	No Hit
GGGTTGACTCCTTCTGGATGTTGTAATCAGAGAGGGTGCGGCCATCTTCC	5	0.125	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	5	0.125	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	5	0.125	No Hit
CAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCT	5	0.125	No Hit
GTAGGGGCTCTGCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGAT	5	0.125	No Hit
GAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCCGCCTTTACAAG	5	0.125	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	5	0.125	No Hit
CGCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGG	5	0.125	No Hit
GGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAG	5	0.125	No Hit
GGGTAAAACCCAGGAGGCCATGGAAGCTGCCTTGGCCATGGAGAAGAACC	5	0.125	No Hit
ATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCC	5	0.125	No Hit
GGATTACTCGGCAGGTCCCAGGAGCCAGTTCCGCATCCCCTGCTGTCCCG	5	0.125	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	5	0.125	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	5	0.125	No Hit
GGCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTC	5	0.125	No Hit
GTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGA	5	0.125	No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0125	0.0
34-35	0.0	0.0	0.0	0.025	0.0
36-37	0.0	0.0	0.0	0.025	0.0
38-39	0.0	0.0	0.0	0.0625	0.0
40-41	0.0	0.0	0.0	0.1	0.0
42-43	0.0	0.0	0.0	0.15	0.0
44-45	0.0	0.0	0.0	0.25	0.0
46-47	0.0	0.0	0.0	0.275	0.0
48-49	0.0	0.0	0.0	0.2875	0.0
50-51	0.0	0.0	0.0	0.42500000000000004	0.0
52-53	0.0	0.0	0.0	0.575	0.0
54-55	0.0	0.0	0.0	0.6375	0.0
56-57	0.0	0.0	0.0	0.8	0.0
58-59	0.0	0.0	0.0	0.9375	0.0
60-61	0.0	0.0	0.0	0.9875	0.0
62-63	0.0	0.0	0.0	1.0499999999999998	0.0
64-65	0.0	0.0	0.0	1.075	0.0
66-67	0.0	0.0	0.0	1.1625	0.0
68-69	0.0	0.0	0.0	1.225	0.0
70-71	0.0	0.0	0.0	1.375	0.0
72-73	0.0	0.0	0.0	1.5125	0.0
74-75	0.0	0.0	0.0	1.65	0.0
76-77	0.0	0.0	0.0	1.8624999999999998	0.0
78-79	0.0	0.0	0.0	1.9874999999999998	0.0
80-81	0.0	0.0	0.0	2.1	0.0
82-83	0.0	0.0	0.0	2.275	0.0
84-85	0.0	0.0	0.0	2.4125	0.0
86-87	0.0	0.0	0.0	2.7125	0.0
88-89	0.0	0.0	0.0	2.925	0.0
90-91	0.0	0.0	0.0	3.1375	0.0
92-93	0.0	0.0	0.0	3.2625	0.0
94-95	0.0	0.0	0.0	3.325	0.0
96-97	0.0	0.0	0.0	3.575	0.0
98-99	0.0	0.0	0.0	3.9	0.0
100-101	0.0	0.0	0.0	4.075	0.0
102-103	0.0	0.0	0.0	4.4	0.0
104-105	0.0	0.0	0.0	4.675	0.0
106-107	0.0	0.0	0.0	5.074999999999999	0.0
108-109	0.0	0.0	0.0	5.4	0.0
110-111	0.0	0.0	0.0	5.6625	0.0
112-113	0.0	0.0	0.0	5.9875	0.0
114-115	0.0	0.0	0.0	6.4125	0.0
116-117	0.0	0.0	0.0	6.550000000000001	0.0
118-119	0.0	0.0	0.0	6.65	0.0
120-121	0.0	0.0	0.0	6.8625	0.0
122-123	0.0	0.0	0.0	7.0875	0.0
124-125	0.0	0.0	0.0	7.3375	0.0
126-127	0.0	0.0	0.0	7.65	0.0
128-129	0.0	0.0	0.0	7.95	0.0
130-131	0.0	0.0	0.0	8.3375	0.0
132-133	0.0	0.0	0.0	8.825	0.0
134-135	0.0	0.0	0.0	9.0625	0.0
136-137	0.0	0.0	0.0	9.4	0.0
138	0.0	0.0	0.0	9.725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	15	1.1730364E-4	144.0	3
GGCGTGC	15	1.1730364E-4	144.0	8
AGGCGTG	15	1.1730364E-4	144.0	7
GCGTGCG	15	1.1730364E-4	144.0	9
ATTAAAG	15	1.1730364E-4	144.0	2
AAAGGCG	15	1.1730364E-4	144.0	5
AAGGCGT	15	1.1730364E-4	144.0	6
TAAAGGC	15	1.1730364E-4	144.0	4
GATTAAA	20	3.687869E-4	108.0	1
TTAAAGG	20	3.687869E-4	108.0	3
TACATGG	25	8.956223E-4	86.399994	2
GTACATG	40	0.005777437	54.0	1
AATCTTT	20	0.006139246	28.8	135-139
GAAGGAT	20	0.006139246	28.8	90-94
TTAAAAC	30	0.0015031899	23.999998	140-144
GGGGGGG	75	1.7189996E-7	19.199999	140-144
>>END_MODULE
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410946 spots for SRR31990345.sra
Written 3410946 spots for SRR31990345.sra
Read 3410951 spots for SRR31990345.sra
Written 3410951 spots for SRR31990345.sra
SRR ids: ['SRR31990345.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hsyge011
SRR31990345.sra spots: 27287573
blocks: [[1, 3410946], [3410947, 6821892], [6821893, 10232838], [10232839, 13643784], [13643785, 17054730], [17054731, 20465676], [20465677, 23876622], [23876623, 27287573]]
SRR31990345 file size 9994668
SRR31990345 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990345 SRR31990345_1.fastq SRR31990345_2.fastq
Input file:	SRR31990345_1.fastq
Paired file:	SRR31990345_2.fastq
trimmed:	SRR31990345-trimmed-pair1.fastq, SRR31990345-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:53:56 2026 >> started

Sat Apr 25 18:54:43 2026 >> done (47.487s)
27287573 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
27287573 (100.00%) read pairs available; of these:
    1629 ( 0.01%) trimmed read pairs available after processing
27285944 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
106	       2	  0.00%
107	       0	  0.00%
108	       0	  0.00%
109	       0	  0.00%
110	       0	  0.00%
111	       0	  0.00%
112	       0	  0.00%
113	       0	  0.00%
114	       0	  0.00%
115	       0	  0.00%
116	       0	  0.00%
117	       0	  0.00%
118	       0	  0.00%
119	       0	  0.00%
120	       0	  0.00%
121	       0	  0.00%
122	       0	  0.00%
123	       0	  0.00%
124	       0	  0.00%
125	       0	  0.00%
126	       0	  0.00%
127	       0	  0.00%
128	       0	  0.00%
129	       0	  0.00%
130	       3	  0.00%
131	       0	  0.00%
132	       0	  0.00%
133	       0	  0.00%
134	       0	  0.00%
135	       0	  0.00%
136	       0	  0.00%
137	       0	  0.00%
138	       0	  0.00%
139	       0	  0.00%
140	       0	  0.00%
141	       0	  0.00%
142	       0	  0.00%
143	       0	  0.00%
144	       1	  0.00%
145	       3	  0.00%
146	       4	  0.00%
147	      54	  0.00%
148	      50	  0.00%
149	    1512	  0.01%
150	27285944	 99.99%
27287573 reads passed initial QC


criterion=sequence-density
sequence-density=9.43
sequence-density-rank=1
fanout-score=35.50
fanout-score-rank=2
prefix-density=10.24
prefix-fanout=32.7
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=24
fanout-score=219.51
fanout-score-rank=1
prefix-density=2.24
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=9.55
sequence-density-rank=1
fanout-score=35.07
fanout-score-rank=2
prefix-density=10.37
prefix-fanout=32.3
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=23
fanout-score=247.04
fanout-score-rank=1
prefix-density=2.25
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA -o SRR31990345 SRR31990345_1.fastq SRR31990345_2.fastq
Input file:	SRR31990345_1.fastq
Paired file:	SRR31990345_2.fastq
trimmed:	SRR31990345-trimmed-pair1.fastq, SRR31990345-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:56:41 2026 >> started

Sat Apr 25 18:57:33 2026 >> done (51.195s)
21830059 read pairs processed; of these:
      83 ( 0.00%) short read pairs filtered out after trimming by size control
      22 ( 0.00%) empty read pairs filtered out after trimming by size control
21829954 (100.00%) read pairs available; of these:
 2833945 (12.98%) trimmed read pairs available after processing
18996009 (87.02%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      16	  0.00%
 19	      26	  0.00%
 20	      16	  0.00%
 21	      43	  0.00%
 22	      29	  0.00%
 23	      75	  0.00%
 24	      54	  0.00%
 25	     163	  0.00%
 26	     142	  0.00%
 27	     238	  0.00%
 28	     430	  0.00%
 29	     574	  0.00%
 30	     806	  0.00%
 31	    1369	  0.01%
 32	    1361	  0.01%
 33	    2290	  0.01%
 34	    1778	  0.01%
 35	    2639	  0.01%
 36	    4528	  0.02%
 37	    5785	  0.03%
 38	    6941	  0.03%
 39	    6538	  0.03%
 40	    7342	  0.03%
 41	    6817	  0.03%
 42	    7458	  0.03%
 43	    7340	  0.03%
 44	   10984	  0.05%
 45	    8697	  0.04%
 46	   11728	  0.05%
 47	   10637	  0.05%
 48	   12844	  0.06%
 49	   10174	  0.05%
 50	   10533	  0.05%
 51	    9925	  0.05%
 52	    9228	  0.04%
 53	   10361	  0.05%
 54	   10386	  0.05%
 55	   15537	  0.07%
 56	   12908	  0.06%
 57	   10783	  0.05%
 58	   10130	  0.05%
 59	    9857	  0.05%
 60	   11554	  0.05%
 61	   11026	  0.05%
 62	   13038	  0.06%
 63	   13504	  0.06%
 64	   10445	  0.05%
 65	   10583	  0.05%
 66	   16925	  0.08%
 67	   15230	  0.07%
 68	   12945	  0.06%
 69	   17236	  0.08%
 70	   14308	  0.07%
 71	   17133	  0.08%
 72	   14904	  0.07%
 73	   14201	  0.07%
 74	   14754	  0.07%
 75	   16176	  0.07%
 76	   15533	  0.07%
 77	   13339	  0.06%
 78	   14939	  0.07%
 79	   14055	  0.06%
 80	   19206	  0.09%
 81	   27446	  0.13%
 82	   23235	  0.11%
 83	   13725	  0.06%
 84	   15068	  0.07%
 85	   16650	  0.08%
 86	   18475	  0.08%
 87	   22235	  0.10%
 88	   19006	  0.09%
 89	   21921	  0.10%
 90	   19713	  0.09%
 91	   17529	  0.08%
 92	   19117	  0.09%
 93	   22780	  0.10%
 94	   20411	  0.09%
 95	   25136	  0.12%
 96	   22439	  0.10%
 97	   19544	  0.09%
 98	   26039	  0.12%
 99	   27452	  0.13%
100	   23710	  0.11%
101	   23485	  0.11%
102	   22988	  0.11%
103	   26310	  0.12%
104	   27909	  0.13%
105	   31580	  0.14%
106	   30374	  0.14%
107	   33448	  0.15%
108	   30303	  0.14%
109	   27848	  0.13%
110	   26308	  0.12%
111	   29637	  0.14%
112	   30630	  0.14%
113	   30391	  0.14%
114	   30003	  0.14%
115	   25741	  0.12%
116	   25476	  0.12%
117	   28155	  0.13%
118	   26630	  0.12%
119	   34047	  0.16%
120	   33324	  0.15%
121	   35393	  0.16%
122	   35151	  0.16%
123	   37345	  0.17%
124	   34779	  0.16%
125	   35624	  0.16%
126	   37919	  0.17%
127	   36481	  0.17%
128	   31067	  0.14%
129	   39288	  0.18%
130	   44660	  0.20%
131	   41446	  0.19%
132	   44299	  0.20%
133	   46571	  0.21%
134	   41974	  0.19%
135	   51082	  0.23%
136	   46385	  0.21%
137	   38851	  0.18%
138	   43057	  0.20%
139	   45104	  0.21%
140	   54722	  0.25%
141	   49517	  0.23%
142	   55764	  0.26%
143	   46857	  0.21%
144	   53173	  0.24%
145	   42562	  0.19%
146	   51508	  0.24%
147	   57119	  0.26%
148	   58991	  0.27%
149	   61790	  0.28%
150	18994713	 87.01%


criterion=sequence-density
sequence-density=1.59
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=29
prefix-density=1.63
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=216.48
fanout-score-rank=1
prefix-density=2.26
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.60
sequence-density-rank=1
fanout-score=2.02
fanout-score-rank=27
prefix-density=1.61
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=29
fanout-score=186.06
fanout-score-rank=1
prefix-density=2.24
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990345 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 18:59:51
                             Started mapping on |	Apr 25 18:59:51
                                    Finished on |	Apr 25 19:03:15
       Mapping speed, Million of reads per hour |	481.54

                          Number of input reads |	27287468
                      Average input read length |	275
                                    UNIQUE READS:
                   Uniquely mapped reads number |	20045177
                        Uniquely mapped reads % |	73.46%
                          Average mapped length |	280.75
                       Number of splices: Total |	17932247
            Number of splices: Annotated (sjdb) |	17821900
                       Number of splices: GT/AG |	17849187
                       Number of splices: GC/AG |	26984
                       Number of splices: AT/AC |	4259
               Number of splices: Non-canonical |	51817
                      Mismatch rate per base, % |	0.27%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.30
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2688327
             % of reads mapped to multiple loci |	9.85%
        Number of reads mapped to too many loci |	60769
             % of reads mapped to too many loci |	0.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.37%
                     % of reads unmapped: other |	0.10%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4553964	4553964	4553964
N_multimapping	2688327	2688327	2688327
N_noFeature	1776245	10136720	10106999
N_ambiguous	2059724	255604	260793
UnstrandedReadsAssigned:16209208 PositiveStrandReadsAssigned:9652853 NegativeStrandReadsAssigned:9677385
Dataset is classified unstranded
MeadianReadLen=142 20thPercentileLength=142 echo kmer=137
SRR31990345 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990345-trimmed-pair1.fastq
                             SRR31990345-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 27,287,468 reads, 21,452,641 reads pseudoaligned
[quant] estimated average fragment length: 230.935
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,633 rounds

 109283 SRR31990345.ke.tsv
  52636 SRR31990345.se.tsv
 161919 total
==> SRR31990345.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	10.3333	0	0
ENSMUST00000196221.1	9	3.33333	0	0
ENSMUST00000179664.1	11	5.33333	0	0
ENSMUST00000178537.1	12	6.33333	0	0
ENSMUST00000178862.1	14	8.33333	0	0
ENSMUST00000179520.1	11	5.33333	0	0
ENSMUST00000179883.1	16	10.3333	0	0
ENSMUST00000195858.1	10	4.33333	0	0
ENSMUST00000179932.1	12	6.33333	0	0

==> SRR31990345.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	5
ENSMUSG00000104017	0
ENSMUSG00000103025	1
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990345 completed mapping pipeline successfully
