Starting /dee2/code/volunteer_pipeline.sh SRR31990344
    current disk space = 61643384
    free memory = 194663236 
SRR31990344 SRAfilesize
a539d4a3ea054ad27bda05cd3a8dc9cb  SRR31990344.sra
SRR31990344.sra file validated
SRR31990344 is paired end
SRR31990344 is conventional basespace
SRR31990344 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03125	37.0	37.0	37.0	37.0	37.0
2	36.13875	37.0	37.0	37.0	37.0	37.0
3	36.1125	37.0	37.0	37.0	37.0	37.0
4	36.296	37.0	37.0	37.0	37.0	37.0
5	36.3395	37.0	37.0	37.0	37.0	37.0
6	36.437	37.0	37.0	37.0	37.0	37.0
7	36.3545	37.0	37.0	37.0	37.0	37.0
8	36.2465	37.0	37.0	37.0	37.0	37.0
9	36.302	37.0	37.0	37.0	37.0	37.0
10-14	36.3267	37.0	37.0	37.0	37.0	37.0
15-19	36.276599999999995	37.0	37.0	37.0	37.0	37.0
20-24	36.2236	37.0	37.0	37.0	37.0	37.0
25-29	36.186699999999995	37.0	37.0	37.0	37.0	37.0
30-34	36.18115	37.0	37.0	37.0	37.0	37.0
35-39	36.1038	37.0	37.0	37.0	37.0	37.0
40-44	35.9957	37.0	37.0	37.0	37.0	37.0
45-49	35.8472	37.0	37.0	37.0	37.0	37.0
50-54	35.8124	37.0	37.0	37.0	37.0	37.0
55-59	35.819599999999994	37.0	37.0	37.0	37.0	37.0
60-64	35.83989999999999	37.0	37.0	37.0	37.0	37.0
65-69	35.8106	37.0	37.0	37.0	37.0	37.0
70-74	35.717	37.0	37.0	37.0	37.0	37.0
75-79	35.7352	37.0	37.0	37.0	37.0	37.0
80-84	35.6894	37.0	37.0	37.0	37.0	37.0
85-89	35.6001	37.0	37.0	37.0	37.0	37.0
90-94	35.5544	37.0	37.0	37.0	37.0	37.0
95-99	35.501099999999994	37.0	37.0	37.0	37.0	37.0
100-104	35.3816	37.0	37.0	37.0	37.0	37.0
105-109	35.2044	37.0	37.0	37.0	34.6	37.0
110-114	35.2274	37.0	37.0	37.0	32.2	37.0
115-119	35.153	37.0	37.0	37.0	32.2	37.0
120-124	35.07	37.0	37.0	37.0	27.4	37.0
125-129	34.80980000000001	37.0	37.0	37.0	25.0	37.0
130-134	34.8806	37.0	37.0	37.0	25.0	37.0
135-139	34.7857	37.0	37.0	37.0	25.0	37.0
140-144	34.596199999999996	37.0	37.0	37.0	25.0	37.0
145-149	34.7022	37.0	37.0	37.0	25.0	37.0
150	34.6935	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	9.0
23	11.0
24	11.0
25	10.0
26	12.0
27	24.0
28	24.0
29	38.0
30	56.0
31	74.0
32	103.0
33	184.0
34	223.0
35	464.0
36	2595.0
37	159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	61.752190237797244	10.187734668335418	4.655819774718398	23.404255319148938
2	19.564673505128845	19.314485864398296	38.779084313234925	22.34175631723793
3	20.45	29.299999999999997	23.95	26.3
4	7.9	9.55	43.85	38.7
5	9.875	42.025	36.449999999999996	11.65
6	33.75	40.9	16.225	9.125
7	29.349999999999998	31.2	21.375	18.075
8	27.875	33.550000000000004	18.675	19.900000000000002
9	27.700000000000003	11.0	13.850000000000001	47.449999999999996
10-14	23.445	22.869999999999997	30.880000000000003	22.805
15-19	23.79	28.325	24.73	23.155
20-24	25.3	25.4	25.195	24.104999999999997
25-29	22.807280728072808	24.70747074707471	26.112611261126112	26.37263726372637
30-34	23.176158807940396	26.73133656682834	25.416270813540677	24.676233811690583
35-39	23.72	26.36	25.924999999999997	23.995
40-44	24.45	27.08	24.79	23.68
45-49	24.27	25.31	25.985000000000003	24.435000000000002
50-54	24.37	25.205	26.06	24.365000000000002
55-59	24.245	24.895	26.405	24.455
60-64	23.94	25.745	26.064999999999998	24.25
65-69	24.93	25.21	25.845000000000002	24.015
70-74	23.27	24.834999999999997	27.27	24.625
75-79	23.825	26.71	24.795	24.67
80-84	24.675	25.27	25.264999999999997	24.79
85-89	24.169999999999998	24.9	25.21	25.72
90-94	24.67	25.31	26.415	23.605
95-99	24.474999999999998	25.545	26.415	23.565
100-104	24.11	25.290000000000003	26.75	23.849999999999998
105-109	22.74	25.295	25.885	26.08
110-114	24.19	25.905	24.6	25.305
115-119	23.89	24.695	26.779999999999998	24.635
120-124	23.86	25.759999999999998	25.5	24.88
125-129	25.395	24.68	26.314999999999998	23.61
130-134	25.095	23.669999999999998	26.36	24.875
135-139	24.785	24.47	25.790000000000003	24.955
140-144	25.45	24.169999999999998	25.814999999999998	24.565
145-149	25.61	24.445	25.074999999999996	24.87
150	24.425	27.775	25.224999999999998	22.575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	0.5
11	1.5
12	2.0
13	0.5
14	0.5
15	1.5
16	1.0
17	0.5
18	1.0
19	1.5
20	2.5
21	3.0
22	2.5
23	6.5
24	9.0
25	9.5
26	20.0
27	25.5
28	28.5
29	27.0
30	17.5
31	20.0
32	26.0
33	34.5
34	47.0
35	61.5
36	78.5
37	78.0
38	82.5
39	96.0
40	106.0
41	130.0
42	119.5
43	82.5
44	80.0
45	78.5
46	92.5
47	107.5
48	116.0
49	175.5
50	172.5
51	145.5
52	195.0
53	218.5
54	211.0
55	204.0
56	189.0
57	156.0
58	144.0
59	153.5
60	126.0
61	91.5
62	64.0
63	39.0
64	29.5
65	29.0
66	24.0
67	16.0
68	8.5
69	3.0
70	1.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.5
76	0.5
77	0.0
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125
2	0.075
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.01
30-34	0.005
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.449999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4869976359338	49.8
2	12.293144208037825	15.6
3	3.6249014972419227	6.9
4	1.5760441292356184	4.0
5	1.1820330969267139	3.75
6	0.5910165484633569	2.25
7	0.4728132387706856	2.1
8	0.1970055161544523	1.0
9	0.2364066193853428	1.35
>10	1.3396375098502757	13.25
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	36	0.8999999999999999	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	25	0.625	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	24	0.6	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	24	0.6	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	22	0.5499999999999999	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	20	0.5	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	20	0.5	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	19	0.475	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	19	0.475	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	18	0.44999999999999996	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	17	0.42500000000000004	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	17	0.42500000000000004	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	16	0.4	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	16	0.4	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	15	0.375	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	15	0.375	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	14	0.35000000000000003	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	14	0.35000000000000003	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	14	0.35000000000000003	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	13	0.325	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	13	0.325	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	12	0.3	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	12	0.3	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	12	0.3	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	11	0.27499999999999997	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	11	0.27499999999999997	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	11	0.27499999999999997	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	10	0.25	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	10	0.25	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	10	0.25	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	10	0.25	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	10	0.25	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	10	0.25	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	10	0.25	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	9	0.22499999999999998	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	9	0.22499999999999998	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	9	0.22499999999999998	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	9	0.22499999999999998	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	9	0.22499999999999998	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	9	0.22499999999999998	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	8	0.2	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	8	0.2	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	8	0.2	No Hit
GTCTCAGAGTTAGTGTGAATGAAGGAACTTAAGAAAGATCAGGACAGGCA	8	0.2	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	8	0.2	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	7	0.17500000000000002	No Hit
GTACATGGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCT	7	0.17500000000000002	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	7	0.17500000000000002	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	7	0.17500000000000002	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	7	0.17500000000000002	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	7	0.17500000000000002	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	7	0.17500000000000002	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	7	0.17500000000000002	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	7	0.17500000000000002	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	7	0.17500000000000002	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	7	0.17500000000000002	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	7	0.17500000000000002	No Hit
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT	6	0.15	No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG	6	0.15	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	6	0.15	No Hit
CTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTG	6	0.15	No Hit
GCCGAGGAGACACAGGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTG	6	0.15	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	6	0.15	No Hit
GTGTAAATCTTCGCTCTGTGTTTCAGGTATCCCAGATGGTAGCCAGGGAC	6	0.15	No Hit
GGACACATTACAACATCACCCCACATCTACAGACACCAAAGCCTCCAACC	6	0.15	No Hit
GTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAGCATCCACA	6	0.15	No Hit
CATTACAACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTAC	6	0.15	No Hit
CCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGGTCCTCGTTGTCGTGG	6	0.15	No Hit
GGACAGGCACAGCTCACTAGTCGCTCCTGAATGCCTCGTGACACTGGGAA	6	0.15	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	6	0.15	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	6	0.15	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	6	0.15	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	5	0.125	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	5	0.125	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	5	0.125	No Hit
GAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTACAC	5	0.125	No Hit
GCACAAAGCCCTGCAGCTCACACGACTGCTGTTTCCCAAGATCAACTGGT	5	0.125	No Hit
GATTTACACTGAAGGACCTGTCAAAGGCTTCCTTGGTAACTTCCAGGAAG	5	0.125	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	5	0.125	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	5	0.125	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	5	0.125	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	5	0.125	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	5	0.125	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	5	0.125	No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA	5	0.125	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	5	0.125	No Hit
CTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGC	5	0.125	No Hit
GCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGC	5	0.125	No Hit
GTACATGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCTC	5	0.125	No Hit
GCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGT	5	0.125	No Hit
GCTTAGAGGTGGGTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTA	5	0.125	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	5	0.125	No Hit
GGCTTGGCCTGGAAGTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGG	5	0.125	No Hit
GTCTAAATCAGAGTGGGAATGGAGGGTAAACAAGGGGAGAAAGAGATGTA	5	0.125	No Hit
GACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCT	5	0.125	No Hit
GCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAACCAGTAT	5	0.125	No Hit
GCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAG	5	0.125	No Hit
GGATTGGACGGGACACTGTGAAGGCCCTGCACGCCTCAGGAGCCAAAGTG	5	0.125	No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA	5	0.125	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	5	0.125	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	5	0.125	No Hit
CTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACATTTGTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.05	0.0
40-41	0.0	0.0	0.0	0.1	0.0
42-43	0.0	0.0	0.0	0.2125	0.0
44-45	0.0	0.0	0.0	0.275	0.0
46-47	0.0	0.0	0.0	0.325	0.0
48-49	0.0	0.0	0.0	0.4625	0.0
50-51	0.0	0.0	0.0	0.55	0.0
52-53	0.0	0.0	0.0	0.6	0.0
54-55	0.0	0.0	0.0	0.7625	0.0
56-57	0.0	0.0	0.0	0.8999999999999999	0.0
58-59	0.0	0.0	0.0	1.025	0.0
60-61	0.0	0.0	0.0	1.0875	0.0
62-63	0.0	0.0	0.0	1.125	0.0
64-65	0.0	0.0	0.0	1.275	0.0
66-67	0.0	0.0	0.0	1.4	0.0
68-69	0.0	0.0	0.0	1.55	0.0
70-71	0.0	0.0	0.0	1.7625000000000002	0.0
72-73	0.0	0.0	0.0	1.9	0.0
74-75	0.0	0.0	0.0	2.0875	0.0
76-77	0.0	0.0	0.0	2.2	0.0
78-79	0.0	0.0	0.0	2.275	0.0
80-81	0.0	0.0	0.0	2.3625	0.0
82-83	0.0	0.0	0.0	2.6375	0.0
84-85	0.0	0.0	0.0	2.75	0.0
86-87	0.0	0.0	0.0	2.9625	0.0
88-89	0.0	0.0	0.0	3.075	0.0
90-91	0.0	0.0	0.0	3.35	0.0
92-93	0.0	0.0	0.0	3.5625	0.0
94-95	0.0	0.0	0.0	3.8499999999999996	0.0
96-97	0.0	0.0	0.0	4.025	0.0
98-99	0.0	0.0	0.0	4.1875	0.0
100-101	0.0	0.0	0.0	4.325	0.0
102-103	0.0	0.0	0.0	4.525	0.0
104-105	0.0	0.0	0.0	4.775	0.0
106-107	0.0	0.0	0.0	5.1125	0.0
108-109	0.0	0.0	0.0	5.3	0.0
110-111	0.0	0.0	0.0	5.5	0.0
112-113	0.0	0.0	0.0	5.7	0.0
114-115	0.0	0.0	0.0	5.9125	0.0
116-117	0.0	0.0	0.0	6.0	0.0
118-119	0.0	0.0	0.0	6.199999999999999	0.0
120-121	0.0	0.0	0.0	6.4875	0.0
122-123	0.0	0.0	0.0	6.65	0.0
124-125	0.0	0.0	0.0	6.875	0.0
126-127	0.0	0.0	0.0	7.074999999999999	0.0
128-129	0.0	0.0	0.0	7.45	0.0
130-131	0.0	0.0	0.0	7.775	0.0
132-133	0.0	0.0	0.0	8.0125	0.0
134-135	0.0	0.0	0.0	8.2375	0.0
136-137	0.0	0.0	0.0	8.524999999999999	0.0
138	0.0	0.0	0.0	8.75	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	15	1.1730364E-4	144.0	5
ACATGGG	20	1.9452218E-6	144.0	3
GTACATG	20	1.9452218E-6	144.0	1
TACATGG	20	1.9452218E-6	144.0	2
CATGGGA	20	1.9452218E-6	144.0	4
TGGGACA	20	3.687869E-4	108.0	6
GGGGGGG	65	1.8189894E-12	28.8	140-144
>>END_MODULE
SRR31990344 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990344_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.352	37.0	37.0	37.0	25.0	37.0
2	35.8925	37.0	37.0	37.0	37.0	37.0
3	35.8825	37.0	37.0	37.0	37.0	37.0
4	35.9225	37.0	37.0	37.0	37.0	37.0
5	36.083	37.0	37.0	37.0	37.0	37.0
6	36.2855	37.0	37.0	37.0	37.0	37.0
7	36.1235	37.0	37.0	37.0	37.0	37.0
8	36.122	37.0	37.0	37.0	37.0	37.0
9	36.184	37.0	37.0	37.0	37.0	37.0
10-14	36.152300000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.153200000000005	37.0	37.0	37.0	37.0	37.0
20-24	36.100899999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.06270000000001	37.0	37.0	37.0	37.0	37.0
30-34	36.053799999999995	37.0	37.0	37.0	37.0	37.0
35-39	36.0395	37.0	37.0	37.0	37.0	37.0
40-44	35.9536	37.0	37.0	37.0	37.0	37.0
45-49	35.8737	37.0	37.0	37.0	37.0	37.0
50-54	35.8797	37.0	37.0	37.0	37.0	37.0
55-59	35.6791	37.0	37.0	37.0	37.0	37.0
60-64	35.7676	37.0	37.0	37.0	37.0	37.0
65-69	35.6605	37.0	37.0	37.0	37.0	37.0
70-74	35.650600000000004	37.0	37.0	37.0	37.0	37.0
75-79	35.5619	37.0	37.0	37.0	37.0	37.0
80-84	35.5032	37.0	37.0	37.0	37.0	37.0
85-89	35.4136	37.0	37.0	37.0	37.0	37.0
90-94	35.4446	37.0	37.0	37.0	37.0	37.0
95-99	35.4881	37.0	37.0	37.0	37.0	37.0
100-104	35.2215	37.0	37.0	37.0	32.2	37.0
105-109	35.3184	37.0	37.0	37.0	34.6	37.0
110-114	35.1685	37.0	37.0	37.0	27.4	37.0
115-119	35.188599999999994	37.0	37.0	37.0	29.8	37.0
120-124	34.9616	37.0	37.0	37.0	25.0	37.0
125-129	34.8219	37.0	37.0	37.0	25.0	37.0
130-134	34.8706	37.0	37.0	37.0	25.0	37.0
135-139	34.782700000000006	37.0	37.0	37.0	25.0	37.0
140-144	34.597500000000004	37.0	37.0	37.0	25.0	37.0
145-149	34.553000000000004	37.0	37.0	37.0	25.0	37.0
150	34.4035	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	7.0
22	16.0
23	4.0
24	8.0
25	12.0
26	11.0
27	25.0
28	21.0
29	42.0
30	48.0
31	61.0
32	76.0
33	142.0
34	304.0
35	738.0
36	2310.0
37	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	59.550000000000004	10.475	5.675	24.3
2	18.925	19.675	38.5	22.900000000000002
3	20.0	29.349999999999998	23.674999999999997	26.974999999999998
4	7.875	9.425	43.675000000000004	39.025
5	10.65	41.55	36.6	11.200000000000001
6	31.674999999999997	42.199999999999996	17.150000000000002	8.975
7	27.450000000000003	31.35	22.475	18.725
8	27.875	31.5	21.425	19.2
9	27.675	11.4	12.275	48.65
10-14	22.875	22.785	31.619999999999997	22.720000000000002
15-19	23.0	28.655	24.925	23.419999999999998
20-24	24.815	25.105	25.64	24.44
25-29	23.330000000000002	25.105	25.869999999999997	25.695
30-34	24.745	26.365	24.65	24.240000000000002
35-39	23.835	26.41	25.825	23.93
40-44	24.310000000000002	26.534999999999997	24.925	24.23
45-49	23.544999999999998	25.455	25.755	25.245
50-54	23.625	25.2	26.515	24.66
55-59	23.794999999999998	25.019999999999996	26.44	24.745
60-64	24.745	25.14	25.424999999999997	24.69
65-69	24.57	25.330000000000002	25.540000000000003	24.560000000000002
70-74	23.985	24.635	26.334999999999997	25.045
75-79	24.64	26.695	24.345	24.32
80-84	25.25	25.72	24.42	24.610000000000003
85-89	24.759999999999998	24.465	25.025	25.75
90-94	25.080000000000002	25.180000000000003	26.085	23.655
95-99	24.985	25.174999999999997	26.76	23.080000000000002
100-104	24.385	25.05	26.505000000000003	24.060000000000002
105-109	23.51	25.069999999999997	26.009999999999998	25.41
110-114	24.77	24.83	25.095	25.305
115-119	24.05	24.529999999999998	27.065	24.355
120-124	24.7	25.61	25.555	24.135
125-129	25.555	24.94	26.14	23.365
130-134	25.14	24.375	26.005	24.48
135-139	24.81	24.795	25.91	24.485
140-144	25.275	24.955	26.490000000000002	23.28
145-149	24.575	24.93	25.745	24.75
150	25.374999999999996	27.55	24.6	22.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	1.0
9	1.0
10	0.5
11	0.5
12	0.0
13	0.5
14	0.5
15	0.0
16	1.0
17	1.0
18	0.0
19	1.5
20	1.5
21	1.0
22	2.0
23	3.5
24	6.0
25	7.0
26	14.0
27	22.5
28	31.5
29	25.0
30	21.5
31	25.0
32	23.0
33	36.5
34	55.5
35	61.5
36	66.0
37	73.5
38	90.0
39	98.0
40	106.0
41	115.5
42	99.0
43	81.5
44	87.0
45	84.5
46	102.0
47	121.0
48	111.0
49	159.5
50	162.5
51	141.5
52	198.0
53	229.0
54	231.5
55	223.0
56	194.5
57	163.0
58	147.5
59	150.5
60	129.5
61	89.5
62	56.5
63	36.5
64	29.0
65	28.5
66	20.5
67	11.0
68	6.0
69	3.0
70	1.5
71	1.0
72	1.0
73	0.5
74	0.0
75	1.0
76	1.5
77	0.5
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.14999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25409197194077	50.2
2	12.548713951675762	16.1
3	3.4294621979735	6.6000000000000005
4	1.9485580670303975	5.0
5	0.9742790335151987	3.125
6	0.779423226812159	3.0
7	0.3117692907248636	1.4000000000000001
8	0.35074045206547155	1.7999999999999998
9	0.23382696804364772	1.35
>10	1.1691348402182384	11.425
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	30	0.75	No Hit
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	30	0.75	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	29	0.7250000000000001	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	25	0.625	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	21	0.525	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	20	0.5	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	19	0.475	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	18	0.44999999999999996	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	15	0.375	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	15	0.375	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	14	0.35000000000000003	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	14	0.35000000000000003	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	14	0.35000000000000003	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	14	0.35000000000000003	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	14	0.35000000000000003	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	13	0.325	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	13	0.325	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	13	0.325	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	12	0.3	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	12	0.3	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	11	0.27499999999999997	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	11	0.27499999999999997	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	10	0.25	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	10	0.25	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	10	0.25	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	10	0.25	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	10	0.25	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	10	0.25	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	10	0.25	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	10	0.25	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	9	0.22499999999999998	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	9	0.22499999999999998	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	9	0.22499999999999998	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	9	0.22499999999999998	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	9	0.22499999999999998	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	9	0.22499999999999998	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	8	0.2	No Hit
CTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTG	8	0.2	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	8	0.2	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	8	0.2	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	8	0.2	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	8	0.2	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	8	0.2	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	8	0.2	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	8	0.2	No Hit
CTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTGTCCATCTGC	7	0.17500000000000002	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	7	0.17500000000000002	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	7	0.17500000000000002	No Hit
GTACATGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCTC	7	0.17500000000000002	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	7	0.17500000000000002	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	7	0.17500000000000002	No Hit
GTACATGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACAT	7	0.17500000000000002	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	7	0.17500000000000002	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	6	0.15	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	6	0.15	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	6	0.15	No Hit
GAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTACAC	6	0.15	No Hit
GTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCTAGCCCC	6	0.15	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	6	0.15	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	6	0.15	No Hit
GTGTTTGAACGGAACAGACCCGGGGATTCCCACTGTACTCGCTTCCAGCC	6	0.15	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	6	0.15	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	6	0.15	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	6	0.15	No Hit
ACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACCTTCGTTT	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	6	0.15	No Hit
GAGTTAGTGTGAATGAAGGAACTTAAGAAAGATCAGGACAGGCACAGCTC	6	0.15	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	6	0.15	No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC	6	0.15	No Hit
CCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGG	6	0.15	No Hit
GGATTGGACGGGACACTGTGAAGGCCCTGCACGCCTCAGGAGCCAAAGTG	6	0.15	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	6	0.15	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC	5	0.125	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	5	0.125	No Hit
TTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGATATAGTA	5	0.125	No Hit
GGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTCTCTTGTGGGAGG	5	0.125	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	5	0.125	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	5	0.125	No Hit
GATTTACACTGAAGGACCTGTCAAAGGCTTCCTTGGTAACTTCCAGGAAG	5	0.125	No Hit
ACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCCTC	5	0.125	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	5	0.125	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	5	0.125	No Hit
GTACATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	5	0.125	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	5	0.125	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	5	0.125	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	5	0.125	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	5	0.125	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	5	0.125	No Hit
CATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAATGAGGAGAAAAGTAT	5	0.125	No Hit
CGTCTATGGTCTGTGAAGCTGCTGTACCTGAGGTGGGGGGTTCCATTCTA	5	0.125	No Hit
GATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTCTCTTGTGGGAGGG	5	0.125	No Hit
ATATTATTCTTACAGCCCCTATATTCATAGCACTTATACTAAATAACTCT	5	0.125	No Hit
CTGTAGGAGTAGATATGAGAGAGGTCACATTCCCTCGATTTCCCCTCTAA	5	0.125	No Hit
GAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTC	5	0.125	No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT	5	0.125	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	5	0.125	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.05	0.0
40-41	0.0	0.0	0.0	0.1	0.0
42-43	0.0	0.0	0.0	0.2125	0.0
44-45	0.0	0.0	0.0	0.275	0.0
46-47	0.0	0.0	0.0	0.325	0.0
48-49	0.0	0.0	0.0	0.4625	0.0
50-51	0.0	0.0	0.0	0.55	0.0
52-53	0.0	0.0	0.0	0.6125	0.0
54-55	0.0	0.0	0.0	0.7875	0.0
56-57	0.0	0.0	0.0	0.925	0.0
58-59	0.0	0.0	0.0	1.0499999999999998	0.0
60-61	0.0	0.0	0.0	1.1125	0.0
62-63	0.0	0.0	0.0	1.15	0.0
64-65	0.0	0.0	0.0	1.3	0.0
66-67	0.0	0.0	0.0	1.425	0.0
68-69	0.0	0.0	0.0	1.5875	0.0
70-71	0.0	0.0	0.0	1.8125	0.0
72-73	0.0	0.0	0.0	1.9500000000000002	0.0
74-75	0.0	0.0	0.0	2.1375	0.0
76-77	0.0	0.0	0.0	2.25	0.0
78-79	0.0	0.0	0.0	2.325	0.0
80-81	0.0	0.0	0.0	2.4375	0.0
82-83	0.0	0.0	0.0	2.7125	0.0
84-85	0.0	0.0	0.0	2.825	0.0
86-87	0.0	0.0	0.0	3.0375	0.0
88-89	0.0	0.0	0.0	3.15	0.0
90-91	0.0	0.0	0.0	3.425	0.0
92-93	0.0	0.0	0.0	3.6375	0.0
94-95	0.0	0.0	0.0	3.925	0.0
96-97	0.0	0.0	0.0	4.1	0.0
98-99	0.0	0.0	0.0	4.2875	0.0
100-101	0.0	0.0	0.0	4.425	0.0
102-103	0.0	0.0	0.0	4.625	0.0
104-105	0.0	0.0	0.0	4.875	0.0
106-107	0.0	0.0	0.0	5.2125	0.0
108-109	0.0	0.0	0.0	5.4	0.0
110-111	0.0	0.0	0.0	5.612500000000001	0.0
112-113	0.0	0.0	0.0	5.85	0.0
114-115	0.0	0.0	0.0	6.0375	0.0
116-117	0.0	0.0	0.0	6.125	0.0
118-119	0.0	0.0	0.0	6.300000000000001	0.0
120-121	0.0	0.0	0.0	6.5875	0.0
122-123	0.0	0.0	0.0	6.775	0.0
124-125	0.0	0.0	0.0	7.050000000000001	0.0
126-127	0.0	0.0	0.0	7.25	0.0
128-129	0.0	0.0	0.0	7.6375	0.0
130-131	0.0	0.0	0.0	7.975	0.0
132-133	0.0	0.0	0.0	8.2375	0.0
134-135	0.0	0.0	0.0	8.4875	0.0
136-137	0.0	0.0	0.0	8.8	0.0
138	0.0	0.0	0.0	9.05	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATGGG	10	0.006973645	144.0	3
GTACATG	10	0.006973645	144.0	1
TACATGG	10	0.006973645	144.0	2
ACCCTGC	10	0.006973645	144.0	1
CCCTGCA	10	0.006973645	144.0	2
ATAGATG	10	0.006973645	144.0	3
GTGAACC	10	0.006973645	144.0	4
TGAACCG	10	0.006973645	144.0	5
>>END_MODULE
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
Read 2540404 spots for SRR31990344.sra
Written 2540404 spots for SRR31990344.sra
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
Read 2540402 spots for SRR31990344.sra
Written 2540402 spots for SRR31990344.sra
SRR ids: ['SRR31990344.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8lttgnnh
SRR31990344.sra spots: 20323218
blocks: [[1, 2540402], [2540403, 5080804], [5080805, 7621206], [7621207, 10161608], [10161609, 12702010], [12702011, 15242412], [15242413, 17782814], [17782815, 20323218]]
SRR31990344 file size 7441052
SRR31990344 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990344 SRR31990344_1.fastq SRR31990344_2.fastq
Input file:	SRR31990344_1.fastq
Paired file:	SRR31990344_2.fastq
trimmed:	SRR31990344-trimmed-pair1.fastq, SRR31990344-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:17:40 2026 >> started

Sat Apr 25 17:18:43 2026 >> done (63.120s)
20323218 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
20323218 (100.00%) read pairs available; of these:
    1066 ( 0.01%) trimmed read pairs available after processing
20322152 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
142	       1	  0.00%
143	       1	  0.00%
144	       1	  0.00%
145	       1	  0.00%
146	       2	  0.00%
147	      28	  0.00%
148	      28	  0.00%
149	    1004	  0.00%
150	20322152	 99.99%
20323218 reads passed initial QC


criterion=sequence-density
sequence-density=8.94
sequence-density-rank=1
fanout-score=34.50
fanout-score-rank=2
prefix-density=9.66
prefix-fanout=32.0
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=24
fanout-score=323.10
fanout-score-rank=1
prefix-density=2.79
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=9.11
sequence-density-rank=1
fanout-score=34.11
fanout-score-rank=2
prefix-density=9.81
prefix-fanout=31.7
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=25
fanout-score=308.47
fanout-score-rank=1
prefix-density=2.80
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA -o SRR31990344 SRR31990344_1.fastq SRR31990344_2.fastq
Input file:	SRR31990344_1.fastq
Paired file:	SRR31990344_2.fastq
trimmed:	SRR31990344-trimmed-pair1.fastq, SRR31990344-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:20:34 2026 >> started

Sat Apr 25 17:21:10 2026 >> done (35.679s)
16258575 read pairs processed; of these:
      65 ( 0.00%) short read pairs filtered out after trimming by size control
      21 ( 0.00%) empty read pairs filtered out after trimming by size control
16258489 (100.00%) read pairs available; of these:
 2037718 (12.53%) trimmed read pairs available after processing
14220771 (87.47%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      10	  0.00%
 19	      17	  0.00%
 20	      13	  0.00%
 21	      29	  0.00%
 22	      34	  0.00%
 23	      69	  0.00%
 24	      45	  0.00%
 25	     159	  0.00%
 26	     127	  0.00%
 27	     173	  0.00%
 28	     396	  0.00%
 29	     532	  0.00%
 30	     712	  0.00%
 31	    1342	  0.01%
 32	    1293	  0.01%
 33	    2143	  0.01%
 34	    1725	  0.01%
 35	    2607	  0.02%
 36	    4366	  0.03%
 37	    5432	  0.03%
 38	    6746	  0.04%
 39	    6279	  0.04%
 40	    7302	  0.04%
 41	    6460	  0.04%
 42	    6880	  0.04%
 43	    6701	  0.04%
 44	   10639	  0.07%
 45	    7966	  0.05%
 46	   10936	  0.07%
 47	    9625	  0.06%
 48	   11816	  0.07%
 49	    9554	  0.06%
 50	    9824	  0.06%
 51	    8359	  0.05%
 52	    8364	  0.05%
 53	    9255	  0.06%
 54	    8802	  0.05%
 55	   14491	  0.09%
 56	   11421	  0.07%
 57	   10036	  0.06%
 58	    9330	  0.06%
 59	    8500	  0.05%
 60	   10306	  0.06%
 61	    9396	  0.06%
 62	   11099	  0.07%
 63	   12136	  0.07%
 64	    9182	  0.06%
 65	    9093	  0.06%
 66	   14109	  0.09%
 67	   13022	  0.08%
 68	   10460	  0.06%
 69	   14892	  0.09%
 70	   12267	  0.08%
 71	   14463	  0.09%
 72	   12182	  0.07%
 73	   11453	  0.07%
 74	   11965	  0.07%
 75	   13584	  0.08%
 76	   12507	  0.08%
 77	   10530	  0.06%
 78	   11829	  0.07%
 79	   10509	  0.06%
 80	   15358	  0.09%
 81	   23318	  0.14%
 82	   19103	  0.12%
 83	   10661	  0.07%
 84	   11194	  0.07%
 85	   12322	  0.08%
 86	   14761	  0.09%
 87	   16386	  0.10%
 88	   15046	  0.09%
 89	   16724	  0.10%
 90	   15180	  0.09%
 91	   13084	  0.08%
 92	   14041	  0.09%
 93	   17527	  0.11%
 94	   14797	  0.09%
 95	   18990	  0.12%
 96	   16722	  0.10%
 97	   14030	  0.09%
 98	   20515	  0.13%
 99	   19470	  0.12%
100	   17350	  0.11%
101	   17110	  0.11%
102	   15695	  0.10%
103	   19097	  0.12%
104	   20891	  0.13%
105	   22821	  0.14%
106	   20239	  0.12%
107	   23541	  0.14%
108	   21758	  0.13%
109	   20560	  0.13%
110	   18288	  0.11%
111	   20712	  0.13%
112	   20603	  0.13%
113	   20674	  0.13%
114	   20736	  0.13%
115	   17907	  0.11%
116	   17527	  0.11%
117	   18730	  0.12%
118	   17999	  0.11%
119	   23848	  0.15%
120	   22228	  0.14%
121	   23513	  0.14%
122	   23695	  0.15%
123	   25612	  0.16%
124	   22486	  0.14%
125	   23796	  0.15%
126	   25649	  0.16%
127	   24556	  0.15%
128	   20208	  0.12%
129	   26995	  0.17%
130	   30888	  0.19%
131	   25816	  0.16%
132	   27627	  0.17%
133	   31113	  0.19%
134	   26409	  0.16%
135	   35177	  0.22%
136	   29753	  0.18%
137	   24386	  0.15%
138	   27808	  0.17%
139	   29824	  0.18%
140	   34994	  0.22%
141	   32212	  0.20%
142	   35316	  0.22%
143	   30909	  0.19%
144	   32864	  0.20%
145	   25933	  0.16%
146	   31973	  0.20%
147	   36357	  0.22%
148	   38543	  0.24%
149	   41096	  0.25%
150	14219944	 87.46%


criterion=sequence-density
sequence-density=1.95
sequence-density-rank=1
fanout-score=2.04
fanout-score-rank=32
prefix-density=1.99
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=326.92
fanout-score-rank=1
prefix-density=2.80
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.96
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=31
prefix-density=1.98
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=282.58
fanout-score-rank=1
prefix-density=2.80
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990344 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 17:22:50
                             Started mapping on |	Apr 25 17:22:50
                                    Finished on |	Apr 25 17:25:06
       Mapping speed, Million of reads per hour |	537.97

                          Number of input reads |	20323132
                      Average input read length |	283
                                    UNIQUE READS:
                   Uniquely mapped reads number |	16021635
                        Uniquely mapped reads % |	78.83%
                          Average mapped length |	285.88
                       Number of splices: Total |	15296614
            Number of splices: Annotated (sjdb) |	15205495
                       Number of splices: GT/AG |	15233956
                       Number of splices: GC/AG |	23886
                       Number of splices: AT/AC |	3229
               Number of splices: Non-canonical |	35543
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.51
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.34
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1758824
             % of reads mapped to multiple loci |	8.65%
        Number of reads mapped to too many loci |	46705
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.19%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2542673	2542673	2542673
N_multimapping	1758824	1758824	1758824
N_noFeature	1393379	8091572	7998622
N_ambiguous	1662613	183267	186543
UnstrandedReadsAssigned:12965643 PositiveStrandReadsAssigned:7746796 NegativeStrandReadsAssigned:7836470
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR31990344 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990344-trimmed-pair1.fastq
                             SRR31990344-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 20,323,132 reads, 16,299,186 reads pseudoaligned
[quant] estimated average fragment length: 248.23
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,101 rounds

 109283 SRR31990344.ke.tsv
  52636 SRR31990344.se.tsv
 161919 total
==> SRR31990344.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR31990344.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	4
ENSMUSG00000104017	5
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990344 completed mapping pipeline successfully
