Starting /dee2/code/volunteer_pipeline.sh SRR31990349
    current disk space = 34300988
    free memory = 202327100 
SRR31990349 SRAfilesize
8674df09944b12719f9feae9e5b3412f  SRR31990349.sra
SRR31990349.sra file validated
SRR31990349 is paired end
SRR31990349 is conventional basespace
SRR31990349 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.073	37.0	37.0	37.0	37.0	37.0
2	36.00875	37.0	37.0	37.0	37.0	37.0
3	36.122	37.0	37.0	37.0	37.0	37.0
4	36.2565	37.0	37.0	37.0	37.0	37.0
5	36.4325	37.0	37.0	37.0	37.0	37.0
6	36.3485	37.0	37.0	37.0	37.0	37.0
7	36.276	37.0	37.0	37.0	37.0	37.0
8	36.314	37.0	37.0	37.0	37.0	37.0
9	36.276	37.0	37.0	37.0	37.0	37.0
10-14	36.2163	37.0	37.0	37.0	37.0	37.0
15-19	36.19930000000001	37.0	37.0	37.0	37.0	37.0
20-24	36.110699999999994	37.0	37.0	37.0	37.0	37.0
25-29	36.0124	37.0	37.0	37.0	37.0	37.0
30-34	35.9619	37.0	37.0	37.0	37.0	37.0
35-39	36.0036	37.0	37.0	37.0	37.0	37.0
40-44	35.8374	37.0	37.0	37.0	37.0	37.0
45-49	35.8206	37.0	37.0	37.0	37.0	37.0
50-54	35.6864	37.0	37.0	37.0	37.0	37.0
55-59	35.64790000000001	37.0	37.0	37.0	37.0	37.0
60-64	35.6268	37.0	37.0	37.0	37.0	37.0
65-69	35.5333	37.0	37.0	37.0	37.0	37.0
70-74	35.483000000000004	37.0	37.0	37.0	37.0	37.0
75-79	35.5413	37.0	37.0	37.0	37.0	37.0
80-84	35.4462	37.0	37.0	37.0	37.0	37.0
85-89	35.3727	37.0	37.0	37.0	37.0	37.0
90-94	35.2695	37.0	37.0	37.0	34.6	37.0
95-99	35.15	37.0	37.0	37.0	29.8	37.0
100-104	35.0938	37.0	37.0	37.0	25.0	37.0
105-109	34.8552	37.0	37.0	37.0	25.0	37.0
110-114	34.7427	37.0	37.0	37.0	25.0	37.0
115-119	34.7065	37.0	37.0	37.0	25.0	37.0
120-124	34.4464	37.0	37.0	37.0	25.0	37.0
125-129	34.403200000000005	37.0	37.0	37.0	25.0	37.0
130-134	34.356500000000004	37.0	37.0	37.0	25.0	37.0
135-139	34.2187	37.0	37.0	37.0	25.0	37.0
140-144	34.1632	37.0	37.0	37.0	25.0	37.0
145-149	33.7944	37.0	37.0	37.0	25.0	37.0
150	34.2435	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	3.0
22	10.0
23	15.0
24	12.0
25	16.0
26	14.0
27	20.0
28	18.0
29	49.0
30	56.0
31	93.0
32	134.0
33	243.0
34	282.0
35	708.0
36	2258.0
37	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.525000000000006	11.825	5.5	25.15
2	17.66474567777499	20.395890754196945	37.584565271861685	24.354798296166376
3	18.099999999999998	29.65	24.8	27.450000000000003
4	7.9	10.025	46.425	35.65
5	9.025	40.225	39.525	11.225
6	32.175	43.775	16.2	7.85
7	29.099999999999998	29.849999999999998	23.25	17.8
8	26.125	33.35	21.925	18.6
9	26.125	12.55	13.325000000000001	48.0
10-14	21.745	23.645	33.06	21.55
15-19	22.325	29.255	25.96	22.46
20-24	23.78	25.919999999999998	26.465	23.835
25-29	22.134999999999998	25.83	27.58	24.455
30-34	23.43	26.924999999999997	27.04	22.605
35-39	23.02	26.865	27.125	22.99
40-44	23.16	27.755000000000003	25.96	23.125
45-49	23.145	25.929999999999996	26.8	24.125
50-54	23.200000000000003	26.040000000000003	27.544999999999998	23.215
55-59	22.96	27.055	27.200000000000003	22.785
60-64	23.915	25.88	27.134999999999998	23.07
65-69	22.34	27.05	26.884999999999998	23.724999999999998
70-74	22.93	26.355	27.139999999999997	23.575
75-79	23.195	27.584999999999997	26.009999999999998	23.21
80-84	22.965	27.0	25.509999999999998	24.525
85-89	23.405	26.755000000000003	25.605	24.235
90-94	23.385	26.72	27.045	22.85
95-99	22.61	27.41	26.865	23.115
100-104	23.49	26.029999999999998	27.215	23.265
105-109	23.095	26.055	26.515	24.335
110-114	23.365	26.105	25.655	24.875
115-119	23.72	25.085	27.765	23.43
120-124	23.919999999999998	26.924999999999997	25.405	23.75
125-129	24.345	25.905	26.43	23.32
130-134	24.775	24.47	26.295	24.46
135-139	24.98	25.25	25.89	23.880000000000003
140-144	24.545	24.515	26.815	24.125
145-149	24.005000000000003	24.27	26.884999999999998	24.84
150	25.900000000000002	25.2	24.6	24.3
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.5
11	0.5
12	0.5
13	1.0
14	0.5
15	0.0
16	1.5
17	1.5
18	1.0
19	2.0
20	3.0
21	2.0
22	2.5
23	7.0
24	7.0
25	10.5
26	29.5
27	46.0
28	44.0
29	33.0
30	31.5
31	36.0
32	44.0
33	61.5
34	88.5
35	96.5
36	95.5
37	93.5
38	101.0
39	112.0
40	140.0
41	175.5
42	131.5
43	86.0
44	104.5
45	113.0
46	113.5
47	120.0
48	107.0
49	126.5
50	136.0
51	126.0
52	165.5
53	199.0
54	179.5
55	162.0
56	153.5
57	126.0
58	115.0
59	121.5
60	96.0
61	64.0
62	42.5
63	27.5
64	30.5
65	28.0
66	22.0
67	13.0
68	5.0
69	3.0
70	3.0
71	2.0
72	1.0
73	1.0
74	1.0
75	1.5
76	1.0
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.22499999999999998
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15097690941386	59.925
2	7.957371225577265	11.200000000000001
3	2.3090586145648313	4.875
4	1.492007104795737	4.2
5	0.6749555950266429	2.375
6	0.5328596802841918	2.25
7	0.39076376554174064	1.925
8	0.24866785079928952	1.4000000000000001
9	0.21314387211367672	1.35
>10	1.0301953818827707	10.5
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	29	0.7250000000000001	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	24	0.6	No Hit
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	21	0.525	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	19	0.475	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	19	0.475	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	18	0.44999999999999996	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	18	0.44999999999999996	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	18	0.44999999999999996	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	17	0.42500000000000004	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	17	0.42500000000000004	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	16	0.4	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	16	0.4	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	13	0.325	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	13	0.325	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	12	0.3	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	12	0.3	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	11	0.27499999999999997	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	11	0.27499999999999997	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	11	0.27499999999999997	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	11	0.27499999999999997	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	11	0.27499999999999997	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	11	0.27499999999999997	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	11	0.27499999999999997	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	11	0.27499999999999997	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	10	0.25	No Hit
CCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGT	10	0.25	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	10	0.25	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	10	0.25	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	10	0.25	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	9	0.22499999999999998	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	9	0.22499999999999998	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	9	0.22499999999999998	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	9	0.22499999999999998	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	9	0.22499999999999998	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	9	0.22499999999999998	No Hit
GGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTG	8	0.2	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	8	0.2	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	8	0.2	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	8	0.2	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	8	0.2	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	8	0.2	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	8	0.2	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	7	0.17500000000000002	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	7	0.17500000000000002	No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC	7	0.17500000000000002	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	7	0.17500000000000002	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	7	0.17500000000000002	No Hit
CAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCT	7	0.17500000000000002	No Hit
GTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCT	7	0.17500000000000002	No Hit
CCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGG	7	0.17500000000000002	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	7	0.17500000000000002	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	7	0.17500000000000002	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	7	0.17500000000000002	No Hit
GTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGGTTACCTAAAT	6	0.15	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	6	0.15	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	6	0.15	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	6	0.15	No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA	6	0.15	No Hit
CATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAATGAGGAGAAAAGTAT	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	6	0.15	No Hit
ATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCC	6	0.15	No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA	6	0.15	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	6	0.15	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	6	0.15	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	6	0.15	No Hit
AGATTGAGCAGTAAAAAGAATTAGAACTCTAAACTTAAGCTAATAGAGTA	6	0.15	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	6	0.15	No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC	5	0.125	No Hit
GTTCTAAAAGGTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGG	5	0.125	No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG	5	0.125	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	5	0.125	No Hit
CTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGGAGAAAATCCT	5	0.125	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	5	0.125	No Hit
CTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCAT	5	0.125	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	5	0.125	No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG	5	0.125	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	5	0.125	No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT	5	0.125	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	5	0.125	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	5	0.125	No Hit
GTATTAGGGCTAAAATTAGGATAGATAAGATTAAGGCTAGGACACCTCCT	5	0.125	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	5	0.125	No Hit
CCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCC	5	0.125	No Hit
GCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGC	5	0.125	No Hit
GCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAG	5	0.125	No Hit
CCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGGTCCTCGTTGTCGTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.025	0.0
38-39	0.0	0.0	0.0	0.1125	0.0
40-41	0.0	0.0	0.0	0.1875	0.0
42-43	0.0	0.0	0.0	0.2875	0.0
44-45	0.0	0.0	0.0	0.3375	0.0
46-47	0.0	0.0	0.0	0.38749999999999996	0.0
48-49	0.0	0.0	0.0	0.5375	0.0
50-51	0.0	0.0	0.0	0.6625	0.0
52-53	0.0	0.0	0.0	0.75	0.0
54-55	0.0	0.0	0.0	0.8374999999999999	0.0
56-57	0.0	0.0	0.0	1.0875	0.0
58-59	0.0	0.0	0.0	1.2125	0.0
60-61	0.0	0.0	0.0	1.35	0.0
62-63	0.0	0.0	0.0	1.5375	0.0
64-65	0.0	0.0	0.0	1.75	0.0
66-67	0.0	0.0	0.0	1.9875	0.0
68-69	0.0	0.0	0.0	2.2	0.0
70-71	0.0	0.0	0.0	2.425	0.0
72-73	0.0	0.0	0.0	2.5375	0.0
74-75	0.0	0.0	0.0	2.7	0.0
76-77	0.0	0.0	0.0	2.875	0.0
78-79	0.0	0.0	0.0	3.1125	0.0
80-81	0.0	0.0	0.0	3.2125000000000004	0.0
82-83	0.0	0.0	0.0	3.6875	0.0
84-85	0.0	0.0	0.0	4.025	0.0
86-87	0.0	0.0	0.0	4.2375	0.0
88-89	0.0	0.0	0.0	4.55	0.0
90-91	0.0	0.0	0.0	4.862500000000001	0.0
92-93	0.0	0.0	0.0	5.0875	0.0
94-95	0.0	0.0	0.0	5.325	0.0
96-97	0.0	0.0	0.0	5.6875	0.0
98-99	0.0	0.0	0.0	6.0875	0.0
100-101	0.0	0.0	0.0	6.4375	0.0
102-103	0.0	0.0	0.0	6.6875	0.0
104-105	0.0	0.0	0.0	6.975	0.0
106-107	0.0	0.0	0.0	7.2875	0.0
108-109	0.0	0.0	0.0	7.9	0.0
110-111	0.0	0.0	0.0	8.337499999999999	0.0
112-113	0.0	0.0	0.0	8.5875	0.0
114-115	0.0	0.0	0.0	8.95	0.0
116-117	0.0	0.0	0.0	9.3375	0.0
118-119	0.0	0.0	0.0	9.625	0.0
120-121	0.0	0.0	0.0	10.0	0.0
122-123	0.0	0.0	0.0	10.45	0.0
124-125	0.0	0.0	0.0	11.025	0.0
126-127	0.0	0.0	0.0	11.350000000000001	0.0
128-129	0.0	0.0	0.0	11.75	0.0
130-131	0.0	0.0	0.0	12.2125	0.0
132-133	0.0	0.0	0.0	12.7625	0.0
134-135	0.0	0.0	0.0	13.375	0.0
136-137	0.0	0.0	0.0	13.925	0.0
138	0.0	0.0	0.0	14.35	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACT	10	0.006973645	144.0	4
ACTGATA	10	0.006973645	144.0	8
GCTAATT	10	0.006973645	144.0	2
CTAATTG	10	0.006973645	144.0	3
ATACGAT	10	0.006973645	144.0	1
TACGATA	10	0.006973645	144.0	2
TAATTGT	10	0.006973645	144.0	4
TACTGAT	10	0.006973645	144.0	7
GGCTAAT	10	0.006973645	144.0	1
ACGATAC	10	0.006973645	144.0	3
ATACTGA	10	0.006973645	144.0	6
CCTAGGC	10	0.006973645	144.0	3
GCACAGC	25	8.956223E-4	86.399994	1
TGAGCTC	25	8.956223E-4	86.399994	8
GGGGGGG	190	2.4304774E-5	9.094737	140-144
>>END_MODULE
SRR31990349 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990349_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.5245	37.0	37.0	37.0	37.0	37.0
2	36.2895	37.0	37.0	37.0	37.0	37.0
3	36.158	37.0	37.0	37.0	37.0	37.0
4	36.348	37.0	37.0	37.0	37.0	37.0
5	36.351	37.0	37.0	37.0	37.0	37.0
6	36.4005	37.0	37.0	37.0	37.0	37.0
7	36.2995	37.0	37.0	37.0	37.0	37.0
8	36.43	37.0	37.0	37.0	37.0	37.0
9	36.3665	37.0	37.0	37.0	37.0	37.0
10-14	36.27595	37.0	37.0	37.0	37.0	37.0
15-19	36.279199999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.2033	37.0	37.0	37.0	37.0	37.0
25-29	36.298199999999994	37.0	37.0	37.0	37.0	37.0
30-34	36.2479	37.0	37.0	37.0	37.0	37.0
35-39	36.19290000000001	37.0	37.0	37.0	37.0	37.0
40-44	36.1331	37.0	37.0	37.0	37.0	37.0
45-49	36.0194	37.0	37.0	37.0	37.0	37.0
50-54	36.0566	37.0	37.0	37.0	37.0	37.0
55-59	35.9093	37.0	37.0	37.0	37.0	37.0
60-64	35.97580000000001	37.0	37.0	37.0	37.0	37.0
65-69	35.8823	37.0	37.0	37.0	37.0	37.0
70-74	35.72130000000001	37.0	37.0	37.0	37.0	37.0
75-79	35.790499999999994	37.0	37.0	37.0	37.0	37.0
80-84	35.7699	37.0	37.0	37.0	37.0	37.0
85-89	35.60510000000001	37.0	37.0	37.0	37.0	37.0
90-94	35.65599999999999	37.0	37.0	37.0	37.0	37.0
95-99	35.5472	37.0	37.0	37.0	37.0	37.0
100-104	35.531099999999995	37.0	37.0	37.0	37.0	37.0
105-109	35.5536	37.0	37.0	37.0	37.0	37.0
110-114	35.4688	37.0	37.0	37.0	37.0	37.0
115-119	35.438900000000004	37.0	37.0	37.0	34.6	37.0
120-124	35.3019	37.0	37.0	37.0	34.6	37.0
125-129	35.2668	37.0	37.0	37.0	34.6	37.0
130-134	34.90480000000001	37.0	37.0	37.0	25.0	37.0
135-139	35.0604	37.0	37.0	37.0	25.0	37.0
140-144	34.99249999999999	37.0	37.0	37.0	27.4	37.0
145-149	34.7087	37.0	37.0	37.0	25.0	37.0
150	34.94	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	7.0
22	7.0
23	13.0
24	10.0
25	17.0
26	11.0
27	13.0
28	20.0
29	25.0
30	39.0
31	53.0
32	73.0
33	110.0
34	227.0
35	592.0
36	2562.0
37	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.3	11.025	5.45	25.224999999999998
2	19.275000000000002	20.775	36.075	23.875
3	20.349999999999998	28.9	24.4	26.35
4	7.449999999999999	11.525	43.275000000000006	37.75
5	9.85	41.375	37.475	11.3
6	31.474999999999998	43.8	15.875	8.85
7	28.425	30.75	22.375	18.45
8	27.224999999999998	35.199999999999996	19.75	17.825
9	27.200000000000003	11.975	13.750000000000002	47.075
10-14	21.801090054502726	23.771188559427973	32.40662033101655	22.021101055052753
15-19	22.575	29.145	25.564999999999998	22.715
20-24	24.05	26.125	26.11	23.715
25-29	23.02	25.41	26.85	24.72
30-34	23.64	27.07	26.35	22.939999999999998
35-39	23.13	27.015	26.735	23.119999999999997
40-44	22.785	28.125	25.735000000000003	23.355
45-49	24.065	26.095000000000002	26.165	23.674999999999997
50-54	23.225	26.87	26.555	23.35
55-59	23.32	26.195	27.185	23.3
60-64	22.994999999999997	26.805	26.325	23.875
65-69	23.355	26.619999999999997	27.04	22.985
70-74	22.935	26.665	26.515	23.885
75-79	23.41	27.775	25.130000000000003	23.685000000000002
80-84	23.895	26.779999999999998	25.5	23.825
85-89	23.74	26.075	25.490000000000002	24.695
90-94	23.544999999999998	26.834999999999997	26.924999999999997	22.695
95-99	23.345	26.905	26.745	23.005
100-104	23.474999999999998	25.814999999999998	27.169999999999998	23.54
105-109	22.32	25.679999999999996	27.125	24.875
110-114	23.39	27.08	25.355	24.175
115-119	23.145	26.13	27.52	23.205000000000002
120-124	22.855	27.134999999999998	26.445	23.565
125-129	23.73	26.939999999999998	26.88	22.45
130-134	24.2	25.05	26.779999999999998	23.97
135-139	24.325	25.869999999999997	26.729999999999997	23.075000000000003
140-144	24.515	25.66	27.250000000000004	22.575
145-149	23.995	25.455	26.815	23.735
150	25.674999999999997	26.900000000000002	25.374999999999996	22.05
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.5
4	0.5
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.5
11	0.5
12	1.5
13	2.0
14	0.5
15	1.0
16	3.0
17	2.5
18	1.5
19	2.0
20	1.5
21	1.0
22	2.0
23	8.5
24	11.0
25	9.5
26	22.5
27	33.0
28	41.5
29	36.0
30	23.5
31	29.0
32	39.5
33	64.5
34	84.0
35	94.0
36	108.5
37	97.0
38	90.5
39	106.0
40	123.0
41	144.5
42	125.5
43	99.0
44	109.5
45	112.0
46	119.0
47	114.0
48	105.0
49	135.0
50	139.5
51	123.5
52	141.5
53	178.5
54	188.0
55	179.5
56	164.0
57	141.0
58	136.0
59	129.0
60	104.0
61	70.5
62	51.0
63	45.0
64	34.5
65	24.0
66	15.0
67	8.0
68	4.0
69	3.0
70	3.5
71	1.5
72	0.0
73	0.5
74	1.0
75	1.0
76	0.5
77	0.0
78	0.0
79	1.0
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.005
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.05
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.37719915552428	59.95
2	8.409570724841661	11.95
3	2.7797325826882475	5.925
4	1.3019000703729768	3.6999999999999997
5	1.0555946516537649	3.75
6	0.3166783954961295	1.35
7	0.633356790992259	3.15
8	0.14074595355383532	0.8
9	0.28149190710767064	1.7999999999999998
>10	0.7037297677691766	7.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	30	0.75	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	26	0.65	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	22	0.5499999999999999	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	22	0.5499999999999999	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	16	0.4	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	16	0.4	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	15	0.375	No Hit
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	15	0.375	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	14	0.35000000000000003	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	14	0.35000000000000003	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	13	0.325	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	13	0.325	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	12	0.3	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	12	0.3	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	11	0.27499999999999997	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	11	0.27499999999999997	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	11	0.27499999999999997	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	11	0.27499999999999997	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	11	0.27499999999999997	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	10	0.25	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	9	0.22499999999999998	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	9	0.22499999999999998	No Hit
GTACATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	9	0.22499999999999998	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	9	0.22499999999999998	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	9	0.22499999999999998	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	9	0.22499999999999998	No Hit
ACCAGACTCTGATTCCATGAGGAGGGCCTCAAGGACTTGAAGAAATCCTG	9	0.22499999999999998	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	9	0.22499999999999998	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	8	0.2	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	8	0.2	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	8	0.2	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	8	0.2	No Hit
GTACATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	7	0.17500000000000002	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	7	0.17500000000000002	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	7	0.17500000000000002	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	7	0.17500000000000002	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	7	0.17500000000000002	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	7	0.17500000000000002	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	7	0.17500000000000002	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	7	0.17500000000000002	No Hit
GGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCATGCA	7	0.17500000000000002	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	7	0.17500000000000002	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	7	0.17500000000000002	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	7	0.17500000000000002	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	7	0.17500000000000002	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	7	0.17500000000000002	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	7	0.17500000000000002	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	7	0.17500000000000002	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	7	0.17500000000000002	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	7	0.17500000000000002	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	6	0.15	No Hit
GATCTTAAGAGATAACATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAA	6	0.15	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	6	0.15	No Hit
CCTTTAATCCTAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCG	6	0.15	No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA	6	0.15	No Hit
CATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAATGAGGAGAAAAGTAT	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT	6	0.15	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	6	0.15	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	5	0.125	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	5	0.125	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	5	0.125	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	5	0.125	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	5	0.125	No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG	5	0.125	No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT	5	0.125	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	5	0.125	No Hit
CCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAACTACATA	5	0.125	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	5	0.125	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	5	0.125	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	5	0.125	No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA	5	0.125	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	5	0.125	No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG	5	0.125	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	5	0.125	No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC	5	0.125	No Hit
GCCTAAGGCTCATAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTA	5	0.125	No Hit
GCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGT	5	0.125	No Hit
ACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACCTTCGTTT	5	0.125	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	5	0.125	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	5	0.125	No Hit
CCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCC	5	0.125	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	5	0.125	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	5	0.125	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	5	0.125	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	5	0.125	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	5	0.125	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	5	0.125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.025	0.0
38-39	0.0	0.0	0.0	0.1125	0.0
40-41	0.0	0.0	0.0	0.1875	0.0
42-43	0.0	0.0	0.0	0.2875	0.0
44-45	0.0	0.0	0.0	0.3375	0.0
46-47	0.0	0.0	0.0	0.38749999999999996	0.0
48-49	0.0	0.0	0.0	0.5375	0.0
50-51	0.0	0.0	0.0	0.6625	0.0
52-53	0.0	0.0	0.0	0.75	0.0
54-55	0.0	0.0	0.0	0.8374999999999999	0.0
56-57	0.0	0.0	0.0	1.0875	0.0
58-59	0.0	0.0	0.0	1.2125	0.0
60-61	0.0	0.0	0.0	1.35	0.0
62-63	0.0	0.0	0.0	1.5375	0.0
64-65	0.0	0.0	0.0	1.75	0.0
66-67	0.0	0.0	0.0	1.9875	0.0
68-69	0.0	0.0	0.0	2.2125	0.0
70-71	0.0	0.0	0.0	2.45	0.0
72-73	0.0	0.0	0.0	2.5625	0.0
74-75	0.0	0.0	0.0	2.7249999999999996	0.0
76-77	0.0	0.0	0.0	2.9	0.0
78-79	0.0	0.0	0.0	3.1375	0.0
80-81	0.0	0.0	0.0	3.2375	0.0
82-83	0.0	0.0	0.0	3.7125000000000004	0.0
84-85	0.0	0.0	0.0	4.050000000000001	0.0
86-87	0.0	0.0	0.0	4.2625	0.0
88-89	0.0	0.0	0.0	4.6	0.0
90-91	0.0	0.0	0.0	4.9375	0.0
92-93	0.0	0.0	0.0	5.1625	0.0
94-95	0.0	0.0	0.0	5.4	0.0
96-97	0.0	0.0	0.0	5.762499999999999	0.0
98-99	0.0	0.0	0.0	6.1625	0.0
100-101	0.0	0.0	0.0	6.512499999999999	0.0
102-103	0.0	0.0	0.0	6.7625	0.0
104-105	0.0	0.0	0.0	7.05	0.0
106-107	0.0	0.0	0.0	7.362500000000001	0.0
108-109	0.0	0.0	0.0	7.987500000000001	0.0
110-111	0.0	0.0	0.0	8.4375	0.0
112-113	0.0	0.0	0.0	8.75	0.0
114-115	0.0	0.0	0.0	9.1125	0.0
116-117	0.0	0.0	0.0	9.4875	0.0
118-119	0.0	0.0	0.0	9.775	0.0
120-121	0.0	0.0	0.0	10.175	0.0
122-123	0.0	0.0	0.0	10.649999999999999	0.0
124-125	0.0	0.0	0.0	11.2125	0.0
126-127	0.0	0.0	0.0	11.55	0.0
128-129	0.0	0.0	0.0	11.925	0.0
130-131	0.0	0.0	0.0	12.375	0.0
132-133	0.0	0.0	0.0	12.9875	0.0
134-135	0.0	0.0	0.0	13.587499999999999	0.0
136-137	0.0	0.0	0.0	14.15	0.0
138	0.0	0.0	0.0	14.6	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGTAC	10	0.006973645	144.0	3
CTGTACC	10	0.006973645	144.0	4
CTCTGTA	10	0.006973645	144.0	2
TTTTTTT	20	0.006139246	28.8	140-144
>>END_MODULE
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
Read 2274188 spots for SRR31990349.sra
Written 2274188 spots for SRR31990349.sra
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
Read 2274183 spots for SRR31990349.sra
Written 2274183 spots for SRR31990349.sra
SRR ids: ['SRR31990349.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_960xf90_
SRR31990349.sra spots: 18193469
blocks: [[1, 2274183], [2274184, 4548366], [4548367, 6822549], [6822550, 9096732], [9096733, 11370915], [11370916, 13645098], [13645099, 15919281], [15919282, 18193469]]
SRR31990349 file size 6677940
SRR31990349 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990349 SRR31990349_1.fastq SRR31990349_2.fastq
Input file:	SRR31990349_1.fastq
Paired file:	SRR31990349_2.fastq
trimmed:	SRR31990349-trimmed-pair1.fastq, SRR31990349-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:02:03 2026 >> started

Sat Apr 25 18:02:41 2026 >> done (37.667s)
18193469 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
18193469 (100.00%) read pairs available; of these:
    1341 ( 0.01%) trimmed read pairs available after processing
18192128 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
145	       3	  0.00%
146	       4	  0.00%
147	      29	  0.00%
148	      54	  0.00%
149	    1251	  0.01%
150	18192128	 99.99%
18193469 reads passed initial QC


criterion=sequence-density
sequence-density=9.69
sequence-density-rank=1
fanout-score=35.93
fanout-score-rank=2
prefix-density=10.65
prefix-fanout=32.7
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=24
fanout-score=94.73
fanout-score-rank=1
prefix-density=1.84
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=9.88
sequence-density-rank=1
fanout-score=35.56
fanout-score-rank=2
prefix-density=10.84
prefix-fanout=32.4
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=22
fanout-score=110.68
fanout-score-rank=1
prefix-density=1.80
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA -o SRR31990349 SRR31990349_1.fastq SRR31990349_2.fastq
Input file:	SRR31990349_1.fastq
Paired file:	SRR31990349_2.fastq
trimmed:	SRR31990349-trimmed-pair1.fastq, SRR31990349-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:03:37 2026 >> started

Sat Apr 25 18:04:04 2026 >> done (27.128s)
14554775 read pairs processed; of these:
      17 ( 0.00%) short read pairs filtered out after trimming by size control
       7 ( 0.00%) empty read pairs filtered out after trimming by size control
14554751 (100.00%) read pairs available; of these:
 2031624 (13.96%) trimmed read pairs available after processing
12523127 (86.04%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       2	  0.00%
 19	       7	  0.00%
 20	       4	  0.00%
 21	      16	  0.00%
 22	      18	  0.00%
 23	      29	  0.00%
 24	      30	  0.00%
 25	      40	  0.00%
 26	      47	  0.00%
 27	     101	  0.00%
 28	     230	  0.00%
 29	     349	  0.00%
 30	     459	  0.00%
 31	     720	  0.00%
 32	     701	  0.00%
 33	    1233	  0.01%
 34	     968	  0.01%
 35	    1418	  0.01%
 36	    2691	  0.02%
 37	    3338	  0.02%
 38	    4192	  0.03%
 39	    4050	  0.03%
 40	    4100	  0.03%
 41	    4267	  0.03%
 42	    4184	  0.03%
 43	    4422	  0.03%
 44	    6799	  0.05%
 45	    5389	  0.04%
 46	    6917	  0.05%
 47	    6402	  0.04%
 48	    7385	  0.05%
 49	    5948	  0.04%
 50	    6773	  0.05%
 51	    5959	  0.04%
 52	    6041	  0.04%
 53	    6000	  0.04%
 54	    6099	  0.04%
 55	    9057	  0.06%
 56	    8154	  0.06%
 57	    6843	  0.05%
 58	    6662	  0.05%
 59	    6458	  0.04%
 60	    7346	  0.05%
 61	    6920	  0.05%
 62	    7606	  0.05%
 63	    8167	  0.06%
 64	    7073	  0.05%
 65	    6803	  0.05%
 66	   10919	  0.08%
 67	    9340	  0.06%
 68	    7783	  0.05%
 69	   10855	  0.07%
 70	    9164	  0.06%
 71	   11061	  0.08%
 72	    9859	  0.07%
 73	    8985	  0.06%
 74	    9675	  0.07%
 75	   10715	  0.07%
 76	   10224	  0.07%
 77	    8923	  0.06%
 78	    9659	  0.07%
 79	    8959	  0.06%
 80	   12360	  0.08%
 81	   18123	  0.12%
 82	   14620	  0.10%
 83	    9336	  0.06%
 84	   10275	  0.07%
 85	   10902	  0.07%
 86	   12532	  0.09%
 87	   14147	  0.10%
 88	   12810	  0.09%
 89	   13806	  0.09%
 90	   13068	  0.09%
 91	   11895	  0.08%
 92	   12738	  0.09%
 93	   16062	  0.11%
 94	   14516	  0.10%
 95	   16782	  0.12%
 96	   17468	  0.12%
 97	   13151	  0.09%
 98	   18491	  0.13%
 99	   18147	  0.12%
100	   16400	  0.11%
101	   17487	  0.12%
102	   15528	  0.11%
103	   18599	  0.13%
104	   20172	  0.14%
105	   22635	  0.16%
106	   20067	  0.14%
107	   21530	  0.15%
108	   23984	  0.16%
109	   19873	  0.14%
110	   18167	  0.12%
111	   22031	  0.15%
112	   21640	  0.15%
113	   22458	  0.15%
114	   21332	  0.15%
115	   18727	  0.13%
116	   19751	  0.14%
117	   21017	  0.14%
118	   19095	  0.13%
119	   24245	  0.17%
120	   25372	  0.17%
121	   25142	  0.17%
122	   25560	  0.18%
123	   31819	  0.22%
124	   25112	  0.17%
125	   25951	  0.18%
126	   28826	  0.20%
127	   27064	  0.19%
128	   23444	  0.16%
129	   29724	  0.20%
130	   40705	  0.28%
131	   29603	  0.20%
132	   32666	  0.22%
133	   38958	  0.27%
134	   32171	  0.22%
135	   34961	  0.24%
136	   35660	  0.25%
137	   28880	  0.20%
138	   36568	  0.25%
139	   33053	  0.23%
140	   39792	  0.27%
141	   38162	  0.26%
142	   42016	  0.29%
143	   37904	  0.26%
144	   41676	  0.29%
145	   32429	  0.22%
146	   39460	  0.27%
147	   42230	  0.29%
148	   51074	  0.35%
149	   46210	  0.32%
150	12522054	 86.03%


criterion=sequence-density
sequence-density=1.55
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=30
prefix-density=1.58
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=92.92
fanout-score-rank=1
prefix-density=1.84
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.56
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=33
prefix-density=1.58
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=35
fanout-score=98.69
fanout-score-rank=1
prefix-density=1.79
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990349 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 18:05:27
                             Started mapping on |	Apr 25 18:05:28
                                    Finished on |	Apr 25 18:07:44
       Mapping speed, Million of reads per hour |	481.59

                          Number of input reads |	18193445
                      Average input read length |	279
                                    UNIQUE READS:
                   Uniquely mapped reads number |	12616277
                        Uniquely mapped reads % |	69.35%
                          Average mapped length |	284.29
                       Number of splices: Total |	10597343
            Number of splices: Annotated (sjdb) |	10517568
                       Number of splices: GT/AG |	10551140
                       Number of splices: GC/AG |	20899
                       Number of splices: AT/AC |	2765
               Number of splices: Non-canonical |	22539
                      Mismatch rate per base, % |	0.31%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.27
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2424028
             % of reads mapped to multiple loci |	13.32%
        Number of reads mapped to too many loci |	40735
             % of reads mapped to too many loci |	0.22%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	17.02%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3153140	3153140	3153140
N_multimapping	2424028	2424028	2424028
N_noFeature	1056698	6336422	6262571
N_ambiguous	1376809	159697	162840
UnstrandedReadsAssigned:10182770 PositiveStrandReadsAssigned:6120158 NegativeStrandReadsAssigned:6190866
Dataset is classified unstranded
MeadianReadLen=142 20thPercentileLength=142 echo kmer=137
SRR31990349 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990349-trimmed-pair1.fastq
                             SRR31990349-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 18,193,445 reads, 14,319,483 reads pseudoaligned
[quant] estimated average fragment length: 224.815
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,126 rounds

 109283 SRR31990349.ke.tsv
  52636 SRR31990349.se.tsv
 161919 total
==> SRR31990349.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	14.5	0	0
ENSMUST00000196221.1	9	7.5	0	0
ENSMUST00000179664.1	11	9.5	0	0
ENSMUST00000178537.1	12	10.5	0	0
ENSMUST00000178862.1	14	12.5	0	0
ENSMUST00000179520.1	11	9.5	0	0
ENSMUST00000179883.1	16	14.5	0	0
ENSMUST00000195858.1	10	8.5	0	0
ENSMUST00000179932.1	12	10.5	0	0

==> SRR31990349.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	3
ENSMUSG00000103377	1
ENSMUSG00000104017	2
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990349 completed mapping pipeline successfully
