Starting /dee2/code/volunteer_pipeline.sh SRR31990353
    current disk space = 36784440
    free memory = 198790504 
SRR31990353 SRAfilesize
0a59d0067c1c1ac79f784481113f9808  SRR31990353.sra
SRR31990353.sra file validated
SRR31990353 is paired end
SRR31990353 is conventional basespace
SRR31990353 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990353_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.968	37.0	37.0	37.0	37.0	37.0
2	36.05175	37.0	37.0	37.0	37.0	37.0
3	36.17	37.0	37.0	37.0	37.0	37.0
4	36.14	37.0	37.0	37.0	37.0	37.0
5	36.448	37.0	37.0	37.0	37.0	37.0
6	36.3465	37.0	37.0	37.0	37.0	37.0
7	36.317	37.0	37.0	37.0	37.0	37.0
8	36.2635	37.0	37.0	37.0	37.0	37.0
9	36.314	37.0	37.0	37.0	37.0	37.0
10-14	36.2441	37.0	37.0	37.0	37.0	37.0
15-19	36.23800000000001	37.0	37.0	37.0	37.0	37.0
20-24	36.142700000000005	37.0	37.0	37.0	37.0	37.0
25-29	36.0292	37.0	37.0	37.0	37.0	37.0
30-34	36.0821	37.0	37.0	37.0	37.0	37.0
35-39	36.0599	37.0	37.0	37.0	37.0	37.0
40-44	35.8731	37.0	37.0	37.0	37.0	37.0
45-49	35.8611	37.0	37.0	37.0	37.0	37.0
50-54	35.826600000000006	37.0	37.0	37.0	37.0	37.0
55-59	35.756600000000006	37.0	37.0	37.0	37.0	37.0
60-64	35.804700000000004	37.0	37.0	37.0	37.0	37.0
65-69	35.601699999999994	37.0	37.0	37.0	37.0	37.0
70-74	35.626000000000005	37.0	37.0	37.0	37.0	37.0
75-79	35.5586	37.0	37.0	37.0	37.0	37.0
80-84	35.5336	37.0	37.0	37.0	37.0	37.0
85-89	35.3859	37.0	37.0	37.0	37.0	37.0
90-94	35.3328	37.0	37.0	37.0	34.6	37.0
95-99	35.1288	37.0	37.0	37.0	27.4	37.0
100-104	34.9731	37.0	37.0	37.0	25.0	37.0
105-109	34.6787	37.0	37.0	37.0	25.0	37.0
110-114	34.6145	37.0	37.0	37.0	25.0	37.0
115-119	34.4423	37.0	37.0	37.0	25.0	37.0
120-124	34.2782	37.0	37.0	37.0	25.0	37.0
125-129	34.168	37.0	37.0	37.0	25.0	37.0
130-134	33.9893	37.0	37.0	37.0	25.0	37.0
135-139	33.8899	37.0	37.0	37.0	25.0	37.0
140-144	33.701800000000006	37.0	37.0	37.0	25.0	37.0
145-149	33.412	37.0	37.0	37.0	19.4	37.0
150	33.417	37.0	37.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	10.0
23	14.0
24	11.0
25	9.0
26	15.0
27	26.0
28	35.0
29	49.0
30	75.0
31	113.0
32	225.0
33	203.0
34	262.0
35	539.0
36	2309.0
37	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.9	11.175	5.475	25.45
2	18.26609872212478	18.992733650714104	38.43648208469055	24.30468554247056
3	20.549999999999997	29.025000000000002	24.275	26.150000000000002
4	7.75	10.7	44.15	37.4
5	10.274999999999999	39.45	39.2	11.075
6	33.1	41.875	16.125	8.9
7	27.900000000000002	31.075000000000003	23.05	17.974999999999998
8	25.974999999999998	34.35	20.325	19.35
9	26.424999999999997	10.65	12.675	50.24999999999999
10-14	22.765	22.42	32.84	21.975
15-19	23.915	28.249999999999996	25.39	22.445
20-24	24.245	25.174999999999997	26.115	24.465
25-29	23.09	25.014999999999997	27.189999999999998	24.705
30-34	24.404999999999998	26.790000000000003	26.314999999999998	22.49
35-39	23.775	26.605	26.515	23.105
40-44	24.035	27.279999999999998	25.545	23.14
45-49	24.75	25.415	26.35	23.485
50-54	23.405	25.935000000000002	26.845000000000002	23.815
55-59	23.82	26.205000000000002	26.295	23.68
60-64	23.56	25.77	26.83	23.84
65-69	23.635	25.405	26.36	24.6
70-74	23.47	24.895	27.534999999999997	24.099999999999998
75-79	24.375	26.41	25.919999999999998	23.294999999999998
80-84	24.32	26.26	24.895	24.525
85-89	23.485	26.38	25.185000000000002	24.95
90-94	23.96	25.81	26.375	23.855
95-99	24.195	25.615	26.235000000000003	23.955000000000002
100-104	24.68	25.695	25.790000000000003	23.835
105-109	23.98	25.465	26.015	24.54
110-114	24.94	25.83	24.87	24.36
115-119	24.91	25.15	26.224999999999998	23.715
120-124	25.55	25.545	25.115	23.79
125-129	27.22	24.525	25.06	23.195
130-134	28.015	24.02	24.154999999999998	23.810000000000002
135-139	27.79	24.235	24.965	23.01
140-144	28.349999999999998	23.9	24.72	23.03
145-149	28.110000000000003	23.79	24.759999999999998	23.34
150	28.275	25.724999999999998	23.075000000000003	22.925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	1.5
11	2.0
12	1.5
13	0.0
14	3.0
15	3.0
16	0.5
17	0.5
18	1.0
19	1.5
20	0.5
21	0.0
22	1.0
23	3.5
24	4.5
25	7.5
26	24.0
27	37.5
28	37.5
29	33.0
30	27.5
31	28.5
32	32.0
33	38.5
34	53.5
35	71.0
36	95.5
37	92.5
38	93.5
39	104.5
40	108.5
41	123.0
42	104.5
43	94.0
44	114.5
45	110.5
46	104.5
47	105.5
48	110.5
49	144.5
50	140.0
51	131.0
52	172.0
53	217.5
54	227.0
55	206.0
56	185.0
57	152.5
58	134.5
59	145.0
60	112.0
61	66.5
62	48.0
63	31.0
64	30.5
65	26.5
66	18.0
67	10.0
68	4.5
69	5.5
70	4.0
71	2.0
72	1.0
73	2.0
74	2.5
75	1.0
76	0.0
77	0.0
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.22499999999999998
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.57657657657658	58.675
2	7.675675675675675	10.65
3	2.990990990990991	6.225
4	1.1531531531531531	3.2
5	0.9729729729729729	3.375
6	0.43243243243243246	1.7999999999999998
7	0.5405405405405406	2.625
8	0.3963963963963964	2.1999999999999997
9	0.36036036036036034	2.25
>10	0.9009009009009009	9.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	25	0.625	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	23	0.575	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	22	0.5499999999999999	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	21	0.525	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	21	0.525	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	20	0.5	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	16	0.4	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	16	0.4	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	16	0.4	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	14	0.35000000000000003	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	14	0.35000000000000003	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	13	0.325	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	12	0.3	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	12	0.3	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	12	0.3	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	12	0.3	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	11	0.27499999999999997	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	10	0.25	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	10	0.25	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	10	0.25	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	10	0.25	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	10	0.25	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	10	0.25	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	10	0.25	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	10	0.25	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	9	0.22499999999999998	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	9	0.22499999999999998	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	9	0.22499999999999998	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	9	0.22499999999999998	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	9	0.22499999999999998	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	9	0.22499999999999998	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	9	0.22499999999999998	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	9	0.22499999999999998	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	9	0.22499999999999998	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	9	0.22499999999999998	No Hit
AGATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCT	8	0.2	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	8	0.2	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	8	0.2	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	8	0.2	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	8	0.2	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	8	0.2	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	8	0.2	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	8	0.2	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	8	0.2	No Hit
AGATTGAGCAGTAAAAAGAATTAGAACTCTAAACTTAAGCTAATAGAGTA	8	0.2	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	8	0.2	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	7	0.17500000000000002	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	7	0.17500000000000002	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	7	0.17500000000000002	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	7	0.17500000000000002	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	7	0.17500000000000002	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	7	0.17500000000000002	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	7	0.17500000000000002	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	7	0.17500000000000002	No Hit
GGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATT	7	0.17500000000000002	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	7	0.17500000000000002	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	7	0.17500000000000002	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	7	0.17500000000000002	No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA	7	0.17500000000000002	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	7	0.17500000000000002	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	7	0.17500000000000002	No Hit
GGGTTGAAAGGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAG	6	0.15	No Hit
TTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGATATAGTA	6	0.15	No Hit
CTCCTAAACTGCTCTGGTCAGCCTCCATTATACAGTACAAATACTTTTCT	6	0.15	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	6	0.15	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	6	0.15	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	6	0.15	No Hit
GGGTAAAACCCAGGAGGCCATGGAAGCTGCCTTGGCCATGGAGAAGAACC	6	0.15	No Hit
GGGCAAAACAGAGACTTCCTCCTCACAAGCTTCAGCTGATGCTGTGTTGT	6	0.15	No Hit
GTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAGCATCCACA	6	0.15	No Hit
GCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGC	6	0.15	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	6	0.15	No Hit
GTACAACACCTATCCCTACTTAGGTGTGATTGAAGGCCAGACTCCTGGAG	6	0.15	No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC	5	0.125	No Hit
GTTCTAAAAGGTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGG	5	0.125	No Hit
GACCATCTCAGTATGTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCC	5	0.125	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	5	0.125	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	5	0.125	No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG	5	0.125	No Hit
GATCTTAAGAGATAACATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAA	5	0.125	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	5	0.125	No Hit
GAGAGACACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAG	5	0.125	No Hit
ACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGAAA	5	0.125	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	5	0.125	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	5	0.125	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	5	0.125	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	5	0.125	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	5	0.125	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	5	0.125	No Hit
GCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGT	5	0.125	No Hit
GAGCCAGGGTTGAAAGGCTTCAGGGATGCCACATAACCAGACTCTGATTC	5	0.125	No Hit
CCTCCAGGGTGATGGTCTTGCCGGTCAGGGTCTTCACGAAGATCTGCATG	5	0.125	No Hit
GGGTCTTTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGAT	5	0.125	No Hit
CAATTACACTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATAT	5	0.125	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	5	0.125	No Hit
ATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCC	5	0.125	No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT	5	0.125	No Hit
GCCTAACACTTCCATCTTCCCTGTTTCCAACTCATGCCTTCAAACGTACA	5	0.125	No Hit
GTACATGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACAT	5	0.125	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.025	0.0
36-37	0.0	0.0	0.0	0.075	0.0
38-39	0.0	0.0	0.0	0.1125	0.0
40-41	0.0	0.0	0.0	0.25	0.0
42-43	0.0	0.0	0.0	0.375	0.0
44-45	0.0	0.0	0.0	0.5125	0.0
46-47	0.0	0.0	0.0	0.7375	0.0
48-49	0.0	0.0	0.0	1.0	0.0
50-51	0.0	0.0	0.0	1.3125	0.0
52-53	0.0	0.0	0.0	1.475	0.0
54-55	0.0	0.0	0.0	1.6625	0.0
56-57	0.0	0.0	0.0	1.925	0.0
58-59	0.0	0.0	0.0	2.0999999999999996	0.0
60-61	0.0	0.0	0.0	2.3	0.0
62-63	0.0	0.0	0.0	2.45	0.0
64-65	0.0	0.0	0.0	2.7125	0.0
66-67	0.0	0.0	0.0	2.9375	0.0
68-69	0.0	0.0	0.0	3.2750000000000004	0.0
70-71	0.0	0.0	0.0	3.6125	0.0
72-73	0.0	0.0	0.0	3.825	0.0
74-75	0.0	0.0	0.0	4.25	0.0
76-77	0.0	0.0	0.0	4.675000000000001	0.0
78-79	0.0	0.0	0.0	4.9125	0.0
80-81	0.0	0.0	0.0	5.525	0.0
82-83	0.0	0.0	0.0	6.137499999999999	0.0
84-85	0.0	0.0	0.0	6.4	0.0
86-87	0.0	0.0	0.0	6.675000000000001	0.0
88-89	0.0	0.0	0.0	7.0375	0.0
90-91	0.0	0.0	0.0	7.4	0.0
92-93	0.0	0.0	0.0	7.75	0.0
94-95	0.0	0.0	0.0	8.1375	0.0
96-97	0.0	0.0	0.0	8.6	0.0
98-99	0.0	0.0	0.0	9.075	0.0
100-101	0.0	0.0	0.0	9.55	0.0
102-103	0.0	0.0	0.0	10.225000000000001	0.0
104-105	0.0	0.0	0.0	10.912500000000001	0.0
106-107	0.0	0.0	0.0	11.5	0.0
108-109	0.0	0.0	0.0	12.0625	0.0
110-111	0.0	0.0	0.0	12.5875	0.0
112-113	0.0	0.0	0.0	13.175	0.0
114-115	0.0	0.0	0.0	14.0125	0.0
116-117	0.0	0.0	0.0	14.662500000000001	0.0
118-119	0.0	0.0	0.0	15.2125	0.0
120-121	0.0	0.0	0.0	15.7	0.0
122-123	0.0	0.0	0.0	16.15	0.0
124-125	0.0	0.0	0.0	16.6625	0.0
126-127	0.0	0.0	0.0	17.25	0.0
128-129	0.0	0.0	0.0	18.1625	0.0
130-131	0.0	0.0	0.0	18.862499999999997	0.0
132-133	0.0	0.0	0.0	19.700000000000003	0.0
134-135	0.0	0.0	0.0	20.4	0.0
136-137	0.0	0.0	0.0	21.35	0.0
138	0.0	0.0	0.0	21.675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	10	0.006973645	144.0	5
CTATACT	10	0.006973645	144.0	4
ACATGGG	20	1.9452218E-6	144.0	3
GTACATG	20	1.9452218E-6	144.0	1
TACATGG	20	1.9452218E-6	144.0	2
CATGGGG	10	0.006973645	144.0	4
CATGGGA	10	0.006973645	144.0	4
GCTTTAT	10	0.006973645	144.0	1
GGGGGGG	730	0.0	7.2986307	140-144
>>END_MODULE
SRR31990353 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990353_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.0815	37.0	37.0	37.0	25.0	37.0
2	36.054	37.0	37.0	37.0	37.0	37.0
3	36.2145	37.0	37.0	37.0	37.0	37.0
4	36.0625	37.0	37.0	37.0	37.0	37.0
5	36.3265	37.0	37.0	37.0	37.0	37.0
6	36.4025	37.0	37.0	37.0	37.0	37.0
7	36.3075	37.0	37.0	37.0	37.0	37.0
8	36.3265	37.0	37.0	37.0	37.0	37.0
9	36.263	37.0	37.0	37.0	37.0	37.0
10-14	36.2625	37.0	37.0	37.0	37.0	37.0
15-19	36.314499999999995	37.0	37.0	37.0	37.0	37.0
20-24	36.2902	37.0	37.0	37.0	37.0	37.0
25-29	36.253400000000006	37.0	37.0	37.0	37.0	37.0
30-34	36.26610000000001	37.0	37.0	37.0	37.0	37.0
35-39	36.2167	37.0	37.0	37.0	37.0	37.0
40-44	36.1444	37.0	37.0	37.0	37.0	37.0
45-49	36.063900000000004	37.0	37.0	37.0	37.0	37.0
50-54	36.023399999999995	37.0	37.0	37.0	37.0	37.0
55-59	35.962300000000006	37.0	37.0	37.0	37.0	37.0
60-64	35.991	37.0	37.0	37.0	37.0	37.0
65-69	36.0195	37.0	37.0	37.0	37.0	37.0
70-74	35.8793	37.0	37.0	37.0	37.0	37.0
75-79	35.8879	37.0	37.0	37.0	37.0	37.0
80-84	35.9177	37.0	37.0	37.0	37.0	37.0
85-89	35.691199999999995	37.0	37.0	37.0	37.0	37.0
90-94	35.745099999999994	37.0	37.0	37.0	37.0	37.0
95-99	35.68	37.0	37.0	37.0	37.0	37.0
100-104	35.582	37.0	37.0	37.0	37.0	37.0
105-109	35.653600000000004	37.0	37.0	37.0	37.0	37.0
110-114	35.40570000000001	37.0	37.0	37.0	37.0	37.0
115-119	35.4833	37.0	37.0	37.0	37.0	37.0
120-124	35.2447	37.0	37.0	37.0	32.2	37.0
125-129	35.165800000000004	37.0	37.0	37.0	32.2	37.0
130-134	34.913	37.0	37.0	37.0	25.0	37.0
135-139	35.038199999999996	37.0	37.0	37.0	25.0	37.0
140-144	34.8386	37.0	37.0	37.0	25.0	37.0
145-149	34.6759	37.0	37.0	37.0	25.0	37.0
150	34.6195	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	2.0
20	0.0
21	6.0
22	10.0
23	12.0
24	14.0
25	10.0
26	5.0
27	16.0
28	26.0
29	36.0
30	39.0
31	60.0
32	78.0
33	128.0
34	229.0
35	467.0
36	2521.0
37	340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.975	10.825	4.9	25.3
2	20.525	20.3	35.525	23.65
3	21.375	26.724999999999998	25.874999999999996	26.025
4	7.6499999999999995	10.95	42.475	38.925
5	10.625	40.8	37.824999999999996	10.75
6	33.425	42.9	15.475	8.200000000000001
7	27.800000000000004	31.825	21.7	18.675
8	26.375	34.9	19.3	19.425
9	27.500000000000004	10.674999999999999	12.3	49.525000000000006
10-14	21.95	23.505000000000003	31.540000000000003	23.005
15-19	23.275000000000002	28.294999999999998	25.05	23.380000000000003
20-24	24.86	25.245	25.8	24.095
25-29	23.36	25.535000000000004	26.38	24.725
30-34	24.09	26.8	25.195	23.915
35-39	23.5	26.56	25.765	24.175
40-44	24.165	26.745	25.064999999999998	24.025
45-49	23.630000000000003	25.540000000000003	25.6	25.230000000000004
50-54	22.975	25.895000000000003	26.590000000000003	24.54
55-59	23.07	26.07	26.015	24.845
60-64	23.65	26.185000000000002	25.919999999999998	24.245
65-69	23.18	25.995	26.525	24.3
70-74	23.095	25.495	26.669999999999998	24.740000000000002
75-79	23.66	26.945000000000004	25.06	24.335
80-84	23.95	25.895000000000003	25.865	24.29
85-89	23.96	25.835	25.0	25.205
90-94	23.9	26.345000000000002	26.724999999999998	23.03
95-99	23.724999999999998	26.31	26.26	23.705000000000002
100-104	24.025	25.729999999999997	26.424999999999997	23.82
105-109	23.035	25.814999999999998	25.8	25.35
110-114	23.494999999999997	26.700000000000003	25.264999999999997	24.54
115-119	23.169999999999998	25.97	26.83	24.03
120-124	23.425	26.685	26.43	23.46
125-129	24.3	25.61	26.575	23.515
130-134	25.28	24.86	26.06	23.799999999999997
135-139	25.025	25.83	26.16	22.985
140-144	25.3	25.685000000000002	25.805	23.21
145-149	24.875	25.840000000000003	25.900000000000002	23.385
150	26.275	27.1	25.525	21.099999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	0.5
12	0.5
13	0.5
14	0.5
15	0.5
16	0.5
17	1.0
18	0.5
19	0.5
20	2.0
21	3.0
22	2.5
23	3.0
24	7.0
25	9.0
26	22.5
27	40.5
28	36.5
29	26.0
30	23.5
31	29.0
32	37.0
33	51.5
34	60.0
35	54.5
36	75.5
37	80.0
38	88.0
39	100.0
40	99.5
41	111.0
42	111.5
43	106.0
44	111.5
45	115.0
46	114.5
47	118.5
48	113.0
49	136.5
50	139.5
51	145.5
52	187.5
53	219.0
54	212.5
55	194.0
56	179.0
57	141.0
58	140.0
59	155.0
60	121.5
61	72.5
62	51.5
63	44.0
64	31.5
65	24.0
66	19.0
67	10.5
68	3.5
69	1.5
70	1.5
71	0.5
72	1.5
73	2.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.17266187050359	58.5
2	7.949640287769784	11.05
3	3.0575539568345325	6.375
4	1.4388489208633095	4.0
5	0.935251798561151	3.25
6	0.5755395683453237	2.4
7	0.4676258992805755	2.275
8	0.2517985611510791	1.4000000000000001
9	0.1798561151079137	1.125
>10	0.9712230215827338	9.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	27	0.675	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	26	0.65	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	25	0.625	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	24	0.6	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	18	0.44999999999999996	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	17	0.42500000000000004	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	15	0.375	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	14	0.35000000000000003	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	14	0.35000000000000003	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	14	0.35000000000000003	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	13	0.325	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	13	0.325	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	13	0.325	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	13	0.325	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	12	0.3	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	12	0.3	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	11	0.27499999999999997	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	11	0.27499999999999997	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	11	0.27499999999999997	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	11	0.27499999999999997	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	11	0.27499999999999997	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	10	0.25	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	10	0.25	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	10	0.25	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	10	0.25	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	10	0.25	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	10	0.25	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	9	0.22499999999999998	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	9	0.22499999999999998	No Hit
ACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACCTTCGTTT	9	0.22499999999999998	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	9	0.22499999999999998	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	9	0.22499999999999998	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	8	0.2	No Hit
GTACATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	8	0.2	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	8	0.2	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	8	0.2	No Hit
GCATTGGCCAGAGCATCGGCGACCTTCTTGCCGTGACCCTTGACCTGGGC	8	0.2	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	8	0.2	No Hit
GTACAACACCTATCCCTACTTAGGTGTGATTGAAGGCCAGACTCCTGGAG	8	0.2	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	7	0.17500000000000002	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	7	0.17500000000000002	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	7	0.17500000000000002	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	7	0.17500000000000002	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	7	0.17500000000000002	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	7	0.17500000000000002	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	7	0.17500000000000002	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	7	0.17500000000000002	No Hit
GGGCAAAACAGAGACTTCCTCCTCACAAGCTTCAGCTGATGCTGTGTTGT	7	0.17500000000000002	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	7	0.17500000000000002	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	7	0.17500000000000002	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	7	0.17500000000000002	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	7	0.17500000000000002	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	6	0.15	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	6	0.15	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	6	0.15	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	6	0.15	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	6	0.15	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	6	0.15	No Hit
CTACAAGGCCGACCTTCAAACTAGAACCTTTTAGAACTTCACAAAACCTC	6	0.15	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	6	0.15	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	6	0.15	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	6	0.15	No Hit
GGGTCTTTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGAT	6	0.15	No Hit
CTATAAGCCAGCTCCAGGAACCTACTGCTACATCATGAAGATGGCTCCAG	6	0.15	No Hit
GGGTAAAACCCAGGAGGCCATGGAAGCTGCCTTGGCCATGGAGAAGAACC	6	0.15	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	6	0.15	No Hit
ATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCACCACCA	6	0.15	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	6	0.15	No Hit
GTCCACCGGATTACTCGGCAGGTCCCAGGAGCCAGTTCCGCATCCCCTGC	5	0.125	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	5	0.125	No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC	5	0.125	No Hit
GATCTTAAGAGATAACATGAAGGCTTATTTAAACAGTTTGAAAAAGGAAA	5	0.125	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	5	0.125	No Hit
GGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTG	5	0.125	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	5	0.125	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	5	0.125	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	5	0.125	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	5	0.125	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	5	0.125	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	5	0.125	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	5	0.125	No Hit
GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT	5	0.125	No Hit
CCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGCATCCCTGA	5	0.125	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	5	0.125	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	5	0.125	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	5	0.125	No Hit
GCCTAACACTTCCATCTTCCCTGTTTCCAACTCATGCCTTCAAACGTACA	5	0.125	No Hit
GTAATATTTCGATAAGCTACTCTATTAGCTTAAGTTTAGAGTTCTAATTC	5	0.125	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	5	0.125	No Hit
GTACAGAGGCCTCCTAGTCGTGCTTGAGAGTGAGGCGCTCAAAGAGATAC	5	0.125	No Hit
GGGCAGCTCTAAGAAAGACAGGTCACGGGTTCCCTGATAAGTTTCTGAGT	5	0.125	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	5	0.125	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	5	0.125	No Hit
CTCCACACCCACCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.025	0.0
36-37	0.0	0.0	0.0	0.075	0.0
38-39	0.0	0.0	0.0	0.1125	0.0
40-41	0.0	0.0	0.0	0.25	0.0
42-43	0.0	0.0	0.0	0.375	0.0
44-45	0.0	0.0	0.0	0.5375	0.0
46-47	0.0	0.0	0.0	0.7625	0.0
48-49	0.0	0.0	0.0	1.025	0.0
50-51	0.0	0.0	0.0	1.3375	0.0
52-53	0.0	0.0	0.0	1.5	0.0
54-55	0.0	0.0	0.0	1.6875	0.0
56-57	0.0	0.0	0.0	1.9375	0.0
58-59	0.0	0.0	0.0	2.0999999999999996	0.0
60-61	0.0	0.0	0.0	2.3	0.0
62-63	0.0	0.0	0.0	2.45	0.0
64-65	0.0	0.0	0.0	2.7125	0.0
66-67	0.0	0.0	0.0	2.925	0.0
68-69	0.0	0.0	0.0	3.25	0.0
70-71	0.0	0.0	0.0	3.5625	0.0
72-73	0.0	0.0	0.0	3.7750000000000004	0.0
74-75	0.0	0.0	0.0	4.2	0.0
76-77	0.0	0.0	0.0	4.625	0.0
78-79	0.0	0.0	0.0	4.8625	0.0
80-81	0.0	0.0	0.0	5.475	0.0
82-83	0.0	0.0	0.0	6.0875	0.0
84-85	0.0	0.0	0.0	6.35	0.0
86-87	0.0	0.0	0.0	6.625	0.0
88-89	0.0	0.0	0.0	7.012499999999999	0.0
90-91	0.0	0.0	0.0	7.4	0.0
92-93	0.0	0.0	0.0	7.75	0.0
94-95	0.0	0.0	0.0	8.1375	0.0
96-97	0.0	0.0	0.0	8.587499999999999	0.0
98-99	0.0	0.0	0.0	9.05	0.0
100-101	0.0	0.0	0.0	9.5125	0.0
102-103	0.0	0.0	0.0	10.175	0.0
104-105	0.0	0.0	0.0	10.875	0.0
106-107	0.0	0.0	0.0	11.475	0.0
108-109	0.0	0.0	0.0	12.037500000000001	0.0
110-111	0.0	0.0	0.0	12.5625	0.0
112-113	0.0	0.0	0.0	13.149999999999999	0.0
114-115	0.0	0.0	0.0	13.9875	0.0
116-117	0.0	0.0	0.0	14.65	0.0
118-119	0.0	0.0	0.0	15.2	0.0
120-121	0.0	0.0	0.0	15.7	0.0
122-123	0.0	0.0	0.0	16.225	0.0
124-125	0.0	0.0	0.0	16.737499999999997	0.0
126-127	0.0	0.0	0.0	17.325000000000003	0.0
128-129	0.0	0.0	0.0	18.2625	0.0
130-131	0.0	0.0	0.0	18.9625	0.0
132-133	0.0	0.0	0.0	19.8375	0.0
134-135	0.0	0.0	0.0	20.6375	0.0
136-137	0.0	0.0	0.0	21.575	0.0
138	0.0	0.0	0.0	21.9	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACA	10	0.006973645	144.0	4
CAGCACA	10	0.006973645	144.0	9
ACATGGG	10	0.006973645	144.0	3
TAATGGA	10	0.006973645	144.0	4
TACATGG	10	0.006973645	144.0	2
ATGGAGG	10	0.006973645	144.0	6
TAGAGCA	10	0.006973645	144.0	4
GCGTAGA	10	0.006973645	144.0	1
GTACAAA	20	3.687869E-4	108.0	1
GGGGGGG	375	1.0713848E-9	8.064	140-144
>>END_MODULE
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
Read 2425217 spots for SRR31990353.sra
Written 2425217 spots for SRR31990353.sra
SRR ids: ['SRR31990353.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_2s1ozgb8
SRR31990353.sra spots: 19401736
blocks: [[1, 2425217], [2425218, 4850434], [4850435, 7275651], [7275652, 9700868], [9700869, 12126085], [12126086, 14551302], [14551303, 16976519], [16976520, 19401736]]
SRR31990353 file size 7122132
SRR31990353 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990353 SRR31990353_1.fastq SRR31990353_2.fastq
Input file:	SRR31990353_1.fastq
Paired file:	SRR31990353_2.fastq
trimmed:	SRR31990353-trimmed-pair1.fastq, SRR31990353-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:08:56 2026 >> started

Sat Apr 25 18:09:30 2026 >> done (33.884s)
19401736 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
19401736 (100.00%) read pairs available; of these:
    1437 ( 0.01%) trimmed read pairs available after processing
19400299 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
143	       1	  0.00%
144	       0	  0.00%
145	       5	  0.00%
146	       3	  0.00%
147	      37	  0.00%
148	      63	  0.00%
149	    1328	  0.01%
150	19400299	 99.99%
19401736 reads passed initial QC


criterion=sequence-density
sequence-density=12.70
sequence-density-rank=1
fanout-score=35.07
fanout-score-rank=1
prefix-density=13.75
prefix-fanout=32.4
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG


criterion=fanout-score
sequence-density=12.70
sequence-density-rank=1
fanout-score=35.07
fanout-score-rank=1
prefix-density=13.75
prefix-fanout=32.4
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG


criterion=sequence-density
sequence-density=12.82
sequence-density-rank=1
fanout-score=34.84
fanout-score-rank=1
prefix-density=13.88
prefix-fanout=32.2
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=12.82
sequence-density-rank=1
fanout-score=34.84
fanout-score-rank=1
prefix-density=13.88
prefix-fanout=32.2
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG -y CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA -o SRR31990353 SRR31990353_1.fastq SRR31990353_2.fastq
Input file:	SRR31990353_1.fastq
Paired file:	SRR31990353_2.fastq
trimmed:	SRR31990353-trimmed-pair1.fastq, SRR31990353-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGG
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:10:28 2026 >> started

Sat Apr 25 18:11:05 2026 >> done (36.784s)
16416854 read pairs processed; of these:
      13 ( 0.00%) short read pairs filtered out after trimming by size control
       3 ( 0.00%) empty read pairs filtered out after trimming by size control
16416838 (100.00%) read pairs available; of these:
 2903861 (17.69%) trimmed read pairs available after processing
13512977 (82.31%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       3	  0.00%
 19	       9	  0.00%
 20	       4	  0.00%
 21	      13	  0.00%
 22	      13	  0.00%
 23	      14	  0.00%
 24	      15	  0.00%
 25	      35	  0.00%
 26	      42	  0.00%
 27	     112	  0.00%
 28	     372	  0.00%
 29	     487	  0.00%
 30	     678	  0.00%
 31	    1083	  0.01%
 32	    1020	  0.01%
 33	    1706	  0.01%
 34	    1489	  0.01%
 35	    2241	  0.01%
 36	    4101	  0.02%
 37	    5114	  0.03%
 38	    5937	  0.04%
 39	    5695	  0.03%
 40	    6796	  0.04%
 41	    6190	  0.04%
 42	    6354	  0.04%
 43	    6434	  0.04%
 44	    9320	  0.06%
 45	    8115	  0.05%
 46	   10488	  0.06%
 47	    9503	  0.06%
 48	   11314	  0.07%
 49	    8987	  0.05%
 50	    9672	  0.06%
 51	    9025	  0.05%
 52	    8420	  0.05%
 53	    9264	  0.06%
 54	    8972	  0.05%
 55	   15100	  0.09%
 56	   11376	  0.07%
 57	   10290	  0.06%
 58	    9424	  0.06%
 59	    9170	  0.06%
 60	   11034	  0.07%
 61	   10548	  0.06%
 62	   12569	  0.08%
 63	   12690	  0.08%
 64	   10845	  0.07%
 65	    9985	  0.06%
 66	   15105	  0.09%
 67	   14460	  0.09%
 68	   12586	  0.08%
 69	   17630	  0.11%
 70	   14170	  0.09%
 71	   16235	  0.10%
 72	   14585	  0.09%
 73	   13250	  0.08%
 74	   14604	  0.09%
 75	   15750	  0.10%
 76	   15210	  0.09%
 77	   13108	  0.08%
 78	   13786	  0.08%
 79	   13754	  0.08%
 80	   19181	  0.12%
 81	   26393	  0.16%
 82	   23493	  0.14%
 83	   13684	  0.08%
 84	   15198	  0.09%
 85	   16467	  0.10%
 86	   18380	  0.11%
 87	   22167	  0.14%
 88	   19096	  0.12%
 89	   22100	  0.13%
 90	   19694	  0.12%
 91	   18231	  0.11%
 92	   19783	  0.12%
 93	   23372	  0.14%
 94	   21000	  0.13%
 95	   24824	  0.15%
 96	   22966	  0.14%
 97	   20275	  0.12%
 98	   27987	  0.17%
 99	   28371	  0.17%
100	   24364	  0.15%
101	   25486	  0.16%
102	   24245	  0.15%
103	   27623	  0.17%
104	   29338	  0.18%
105	   34580	  0.21%
106	   31641	  0.19%
107	   33460	  0.20%
108	   31682	  0.19%
109	   29090	  0.18%
110	   28606	  0.17%
111	   31934	  0.19%
112	   32346	  0.20%
113	   32599	  0.20%
114	   31444	  0.19%
115	   27107	  0.17%
116	   26884	  0.16%
117	   29725	  0.18%
118	   28140	  0.17%
119	   35739	  0.22%
120	   35907	  0.22%
121	   38002	  0.23%
122	   37306	  0.23%
123	   39236	  0.24%
124	   37588	  0.23%
125	   36725	  0.22%
126	   40390	  0.25%
127	   40010	  0.24%
128	   32760	  0.20%
129	   44750	  0.27%
130	   47605	  0.29%
131	   43816	  0.27%
132	   46290	  0.28%
133	   48643	  0.30%
134	   43926	  0.27%
135	   52925	  0.32%
136	   48638	  0.30%
137	   40129	  0.24%
138	   46076	  0.28%
139	   46429	  0.28%
140	   57430	  0.35%
141	   52039	  0.32%
142	   55406	  0.34%
143	   50016	  0.30%
144	   54848	  0.33%
145	   45172	  0.28%
146	   54264	  0.33%
147	   58241	  0.35%
148	   62595	  0.38%
149	   62930	  0.38%
150	13511750	 82.30%


criterion=sequence-density
sequence-density=1.19
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=27
prefix-density=1.21
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=279.19
fanout-score-rank=1
prefix-density=2.02
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.19
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=28
prefix-density=1.20
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=255.82
fanout-score-rank=1
prefix-density=1.95
prefix-fanout=1.1
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990353 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 18:12:17
                             Started mapping on |	Apr 25 18:12:17
                                    Finished on |	Apr 25 18:14:32
       Mapping speed, Million of reads per hour |	517.38

                          Number of input reads |	19401720
                      Average input read length |	280
                                    UNIQUE READS:
                   Uniquely mapped reads number |	14462349
                        Uniquely mapped reads % |	74.54%
                          Average mapped length |	283.21
                       Number of splices: Total |	14031633
            Number of splices: Annotated (sjdb) |	13935773
                       Number of splices: GT/AG |	13967597
                       Number of splices: GC/AG |	24396
                       Number of splices: AT/AC |	3857
               Number of splices: Non-canonical |	35783
                      Mismatch rate per base, % |	0.30%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.33
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1883152
             % of reads mapped to multiple loci |	9.71%
        Number of reads mapped to too many loci |	36959
             % of reads mapped to too many loci |	0.19%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	15.49%
                     % of reads unmapped: other |	0.08%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3056219	3056219	3056219
N_multimapping	1883152	1883152	1883152
N_noFeature	1208498	7371730	7295956
N_ambiguous	1453474	231678	244065
UnstrandedReadsAssigned:11800377 PositiveStrandReadsAssigned:6858941 NegativeStrandReadsAssigned:6922328
Dataset is classified unstranded
MeadianReadLen=142 20thPercentileLength=142 echo kmer=137
SRR31990353 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990353-trimmed-pair1.fastq
                             SRR31990353-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 19,401,720 reads, 15,912,913 reads pseudoaligned
[quant] estimated average fragment length: 218.807
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,047 rounds

 109283 SRR31990353.ke.tsv
  52636 SRR31990353.se.tsv
 161919 total
==> SRR31990353.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	2	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	4	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR31990353.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990353 completed mapping pipeline successfully
