Starting /dee2/code/volunteer_pipeline.sh SRR31990342
    current disk space = 93131268
    free memory = 200979000 
SRR31990342 SRAfilesize
15d2182c314ad03cf468987f98c66c90  SRR31990342.sra
SRR31990342.sra file validated
SRR31990342 is paired end
SRR31990342 is conventional basespace
SRR31990342 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.21275	37.0	37.0	37.0	37.0	37.0
2	36.2765	37.0	37.0	37.0	37.0	37.0
3	36.2405	37.0	37.0	37.0	37.0	37.0
4	36.3155	37.0	37.0	37.0	37.0	37.0
5	36.5455	37.0	37.0	37.0	37.0	37.0
6	36.4485	37.0	37.0	37.0	37.0	37.0
7	36.4325	37.0	37.0	37.0	37.0	37.0
8	36.4565	37.0	37.0	37.0	37.0	37.0
9	36.4625	37.0	37.0	37.0	37.0	37.0
10-14	36.403600000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.4367	37.0	37.0	37.0	37.0	37.0
20-24	36.3577	37.0	37.0	37.0	37.0	37.0
25-29	36.35745	37.0	37.0	37.0	37.0	37.0
30-34	36.25320000000001	37.0	37.0	37.0	37.0	37.0
35-39	36.211	37.0	37.0	37.0	37.0	37.0
40-44	36.1337	37.0	37.0	37.0	37.0	37.0
45-49	35.9985	37.0	37.0	37.0	37.0	37.0
50-54	35.9626	37.0	37.0	37.0	37.0	37.0
55-59	35.9748	37.0	37.0	37.0	37.0	37.0
60-64	35.953199999999995	37.0	37.0	37.0	37.0	37.0
65-69	35.9051	37.0	37.0	37.0	37.0	37.0
70-74	35.8949	37.0	37.0	37.0	37.0	37.0
75-79	35.8669	37.0	37.0	37.0	37.0	37.0
80-84	35.888200000000005	37.0	37.0	37.0	37.0	37.0
85-89	35.71419999999999	37.0	37.0	37.0	37.0	37.0
90-94	35.5567	37.0	37.0	37.0	37.0	37.0
95-99	35.5005	37.0	37.0	37.0	37.0	37.0
100-104	35.3636	37.0	37.0	37.0	37.0	37.0
105-109	35.1237	37.0	37.0	37.0	29.8	37.0
110-114	35.2264	37.0	37.0	37.0	34.6	37.0
115-119	35.1011	37.0	37.0	37.0	32.2	37.0
120-124	34.938300000000005	37.0	37.0	37.0	25.0	37.0
125-129	34.7736	37.0	37.0	37.0	25.0	37.0
130-134	34.828599999999994	37.0	37.0	37.0	25.0	37.0
135-139	34.7909	37.0	37.0	37.0	25.0	37.0
140-144	34.6515	37.0	37.0	37.0	25.0	37.0
145-149	34.67	37.0	37.0	37.0	25.0	37.0
150	34.579	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	4.0
22	9.0
23	6.0
24	3.0
25	8.0
26	16.0
27	14.0
28	22.0
29	37.0
30	54.0
31	75.0
32	147.0
33	186.0
34	180.0
35	435.0
36	2621.0
37	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	60.120090067550656	10.007505629221916	5.1538653990492875	24.718538904178132
2	21.160580290145074	19.534767383691847	36.743371685842924	22.56128064032016
3	21.4	27.150000000000002	24.6	26.85
4	8.25	11.450000000000001	44.775	35.525
5	11.1	39.300000000000004	37.574999999999996	12.025
6	33.025	42.75	15.75	8.475000000000001
7	28.775000000000002	29.925	21.875	19.425
8	25.924999999999997	34.575	19.675	19.825
9	26.35	11.0	12.6	50.05
10-14	22.74	22.675	31.615	22.97
15-19	23.665	28.439999999999998	24.834999999999997	23.06
20-24	25.09	25.580000000000002	24.75	24.58
25-29	23.54853227984198	25.33880082012302	25.80387058058709	25.308796319447918
30-34	24.86	25.83	25.224999999999998	24.085
35-39	24.46	26.505000000000003	25.61	23.425
40-44	23.805	26.365	25.16	24.67
45-49	25.025	24.975	25.395	24.605
50-54	22.78	25.505	26.655	25.06
55-59	24.395	25.569999999999997	25.569999999999997	24.465
60-64	23.945	25.759999999999998	26.405	23.89
65-69	24.68	25.605	25.395	24.32
70-74	24.11	25.4	25.94	24.55
75-79	24.69	26.3	24.815	24.195
80-84	24.740000000000002	25.974999999999998	24.240000000000002	25.045
85-89	24.01	24.905	24.46	26.625
90-94	24.29	25.845000000000002	25.665	24.2
95-99	24.759999999999998	25.345000000000002	25.650000000000002	24.245
100-104	25.3	24.81	25.795	24.095
105-109	23.61	24.845	25.009999999999998	26.534999999999997
110-114	25.224999999999998	25.09	24.19	25.495
115-119	24.85	24.23	25.979999999999997	24.94
120-124	25.130000000000003	25.515	24.975	24.38
125-129	26.095000000000002	24.52	25.624999999999996	23.76
130-134	25.345000000000002	24.0	25.355	25.3
135-139	26.02	24.14	25.16	24.68
140-144	27.095000000000002	23.625	24.86	24.42
145-149	26.889999999999997	24.08	23.52	25.509999999999998
150	27.675	26.224999999999998	22.45	23.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	1.5
18	0.5
19	0.5
20	1.0
21	1.0
22	2.5
23	6.5
24	5.5
25	3.5
26	10.5
27	19.0
28	23.0
29	25.0
30	20.5
31	13.5
32	20.0
33	44.5
34	62.5
35	63.5
36	73.0
37	77.0
38	80.5
39	87.5
40	97.0
41	112.5
42	100.5
43	94.0
44	111.5
45	107.0
46	111.5
47	111.5
48	112.0
49	163.0
50	155.5
51	129.0
52	193.0
53	240.0
54	228.0
55	209.5
56	211.5
57	176.5
58	132.0
59	141.5
60	129.5
61	80.0
62	46.0
63	37.0
64	39.0
65	36.0
66	20.0
67	11.5
68	7.0
69	3.5
70	2.0
71	1.5
72	1.0
73	1.0
74	0.5
75	0.0
76	0.0
77	0.0
78	1.0
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.015
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.824999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1226008617313	50.5
2	11.711711711711711	14.95
3	3.3294163728946335	6.375
4	1.410105757931845	3.5999999999999996
5	0.9792401096748923	3.125
6	1.1750881316098707	4.5
7	0.5483744614179397	2.45
8	0.27418723070896983	1.4000000000000001
9	0.23501762632197415	1.35
>10	1.2142577359968665	11.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	35	0.8750000000000001	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	33	0.8250000000000001	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	24	0.6	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	22	0.5499999999999999	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	19	0.475	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	18	0.44999999999999996	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	17	0.42500000000000004	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	17	0.42500000000000004	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	17	0.42500000000000004	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	17	0.42500000000000004	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	16	0.4	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	16	0.4	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	15	0.375	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	14	0.35000000000000003	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	14	0.35000000000000003	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	13	0.325	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	12	0.3	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	12	0.3	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	12	0.3	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	12	0.3	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	12	0.3	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	11	0.27499999999999997	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	11	0.27499999999999997	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	11	0.27499999999999997	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	10	0.25	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	10	0.25	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	10	0.25	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	10	0.25	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	10	0.25	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	10	0.25	No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT	10	0.25	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	9	0.22499999999999998	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	9	0.22499999999999998	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	9	0.22499999999999998	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	9	0.22499999999999998	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	9	0.22499999999999998	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	9	0.22499999999999998	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	8	0.2	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	8	0.2	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	8	0.2	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	8	0.2	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	8	0.2	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	8	0.2	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	8	0.2	No Hit
GGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTCTCTTGTGGGAGG	7	0.17500000000000002	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	7	0.17500000000000002	No Hit
GAGAGACACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAG	7	0.17500000000000002	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	7	0.17500000000000002	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	7	0.17500000000000002	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	7	0.17500000000000002	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	7	0.17500000000000002	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	7	0.17500000000000002	No Hit
GTTGAAAGGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGA	7	0.17500000000000002	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	7	0.17500000000000002	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	7	0.17500000000000002	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	7	0.17500000000000002	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	7	0.17500000000000002	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	7	0.17500000000000002	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	6	0.15	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	6	0.15	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	6	0.15	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	6	0.15	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	6	0.15	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	6	0.15	No Hit
GTGTTGTACTTCTTTGTAATGCTTGCGATGGCCTCGTTCTCCTCAGGATT	6	0.15	No Hit
ATCTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGC	6	0.15	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	6	0.15	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	6	0.15	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	6	0.15	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	6	0.15	No Hit
GAGCAGAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTC	6	0.15	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	6	0.15	No Hit
CCTAAATAGAATCTAAGTGGCATTTAAAACAGTAAAGTTGTAGAGAATAG	6	0.15	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	6	0.15	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	6	0.15	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	6	0.15	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	6	0.15	No Hit
GTACATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	6	0.15	No Hit
CCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGG	6	0.15	No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA	6	0.15	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	6	0.15	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	6	0.15	No Hit
GAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTC	6	0.15	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	6	0.15	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	6	0.15	No Hit
GGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATT	6	0.15	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	5	0.125	No Hit
GAATAGTACAGTGCCCGAGAAGAAAAACTTGACGAGTCCCGTTGGGGTCC	5	0.125	No Hit
GAATAATGTCGTCCAAAGGTACAGGTAATTTGTCAACAACAGGGAGACGG	5	0.125	No Hit
CTCTACAACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACC	5	0.125	No Hit
ACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGAAA	5	0.125	No Hit
GTTTGATCCTGTTTCGTGGAGGAAGAGGAGGTGAACGATTGCTAGGGCCG	5	0.125	No Hit
GCTTTGTGCAGTCCCAGCACCCAGTTAGAACAGCCTCTGTGGTGGGCCGG	5	0.125	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	5	0.125	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	5	0.125	No Hit
GGGTTGACTCCTTCTGGATGTTGTAATCAGAGAGGGTGCGGCCATCTTCC	5	0.125	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	5	0.125	No Hit
GCCTTAGGCTTTATTTTCTTATTTACAGTTGGTGGTCTAACCGGAATTGT	5	0.125	No Hit
ACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACCTTCGTTT	5	0.125	No Hit
GCCCAAAAGAGAGTCCAGGAACGCAACAAGCCTGCCTACGAGATCAACAG	5	0.125	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	5	0.125	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	5	0.125	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	5	0.125	No Hit
GCTCAGCTTCTTCGGACATCTGCCCAGGATTTCTTCAAGTCCTTGAGGCC	5	0.125	No Hit
GGACAGAAGTTTGTGCCGGAAGCCCTGGGATCCCTGGCACTCCCGGAAAC	5	0.125	No Hit
CTATAAGCCAGCTCCAGGAACCTACTGCTACATCATGAAGATGGCTCCAG	5	0.125	No Hit
GGGTAAAACCCAGGAGGCCATGGAAGCTGCCTTGGCCATGGAGAAGAACC	5	0.125	No Hit
GGGTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTT	5	0.125	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	5	0.125	No Hit
CCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCTGCTTGTC	5	0.125	No Hit
TTTCTACACATTTCTCTTTGCCCCGACCCCTGGGCTCTCCTGGGTACCAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.05	0.0
30-31	0.0	0.0	0.0	0.05	0.0
32-33	0.0	0.0	0.0	0.05	0.0
34-35	0.0	0.0	0.0	0.075	0.0
36-37	0.0	0.0	0.0	0.125	0.0
38-39	0.0	0.0	0.0	0.25	0.0
40-41	0.0	0.0	0.0	0.425	0.0
42-43	0.0	0.0	0.0	0.5875	0.0
44-45	0.0	0.0	0.0	0.7875	0.0
46-47	0.0	0.0	0.0	0.9874999999999999	0.0
48-49	0.0	0.0	0.0	1.1	0.0
50-51	0.0	0.0	0.0	1.225	0.0
52-53	0.0	0.0	0.0	1.4625	0.0
54-55	0.0	0.0	0.0	1.6125	0.0
56-57	0.0	0.0	0.0	1.775	0.0
58-59	0.0	0.0	0.0	1.8875	0.0
60-61	0.0	0.0	0.0	2.0375	0.0
62-63	0.0	0.0	0.0	2.2625	0.0
64-65	0.0	0.0	0.0	2.4125	0.0
66-67	0.0	0.0	0.0	2.6375	0.0
68-69	0.0	0.0	0.0	2.875	0.0
70-71	0.0	0.0	0.0	3.0999999999999996	0.0
72-73	0.0	0.0	0.0	3.45	0.0
74-75	0.0	0.0	0.0	3.65	0.0
76-77	0.0	0.0	0.0	3.8375	0.0
78-79	0.0	0.0	0.0	3.95	0.0
80-81	0.0	0.0	0.0	4.137499999999999	0.0
82-83	0.0	0.0	0.0	4.449999999999999	0.0
84-85	0.0	0.0	0.0	4.725	0.0
86-87	0.0	0.0	0.0	5.0375	0.0
88-89	0.0	0.0	0.0	5.2125	0.0
90-91	0.0	0.0	0.0	5.4375	0.0
92-93	0.0	0.0	0.0	5.65	0.0
94-95	0.0	0.0	0.0	5.9625	0.0
96-97	0.0	0.0	0.0	6.2875	0.0
98-99	0.0	0.0	0.0	6.475	0.0
100-101	0.0	0.0	0.0	6.8125	0.0
102-103	0.0	0.0	0.0	7.025	0.0
104-105	0.0	0.0	0.0	7.2625	0.0
106-107	0.0	0.0	0.0	7.6	0.0
108-109	0.0	0.0	0.0	7.95	0.0
110-111	0.0	0.0	0.0	8.2	0.0
112-113	0.0	0.0	0.0	8.7	0.0
114-115	0.0	0.0	0.0	9.275	0.0
116-117	0.0	0.0	0.0	9.8125	0.0
118-119	0.0	0.0	0.0	10.275	0.0
120-121	0.0	0.0	0.0	10.775	0.0
122-123	0.0	0.0	0.0	11.25	0.0
124-125	0.0	0.0	0.0	11.837499999999999	0.0
126-127	0.0	0.0	0.0	12.350000000000001	0.0
128-129	0.0	0.0	0.0	12.8125	0.0
130-131	0.0	0.0	0.0	13.35	0.0
132-133	0.0	0.0	0.0	13.9125	0.0
134-135	0.0	0.0	0.0	14.45	0.0
136-137	0.0	0.0	0.0	15.2625	0.0
138	0.0	0.0	0.0	15.625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	10	0.006716492	145.79747	1
TATTAGG	10	0.006716492	145.79747	2
ATTAGGG	10	0.0069772652	143.975	3
AGGGCTA	10	0.0069772652	143.975	6
ATTATCC	10	0.0069772652	143.975	3
GCTAAAA	10	0.0069772652	143.975	9
TTAGGGC	10	0.0069772652	143.975	4
GGCTAAA	10	0.0069772652	143.975	8
TAGGGCT	10	0.0069772652	143.975	5
>>END_MODULE
SRR31990342 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990342_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.331	37.0	37.0	37.0	25.0	37.0
2	35.917	37.0	37.0	37.0	37.0	37.0
3	35.8845	37.0	37.0	37.0	37.0	37.0
4	35.924	37.0	37.0	37.0	37.0	37.0
5	36.102	37.0	37.0	37.0	37.0	37.0
6	36.05	37.0	37.0	37.0	37.0	37.0
7	35.9895	37.0	37.0	37.0	37.0	37.0
8	36.0885	37.0	37.0	37.0	37.0	37.0
9	36.06	37.0	37.0	37.0	37.0	37.0
10-14	36.1065	37.0	37.0	37.0	37.0	37.0
15-19	36.0598	37.0	37.0	37.0	37.0	37.0
20-24	35.9836	37.0	37.0	37.0	37.0	37.0
25-29	36.031000000000006	37.0	37.0	37.0	37.0	37.0
30-34	35.9969	37.0	37.0	37.0	37.0	37.0
35-39	35.9865	37.0	37.0	37.0	37.0	37.0
40-44	35.8563	37.0	37.0	37.0	37.0	37.0
45-49	35.7952	37.0	37.0	37.0	37.0	37.0
50-54	35.8067	37.0	37.0	37.0	37.0	37.0
55-59	35.6519	37.0	37.0	37.0	37.0	37.0
60-64	35.7433	37.0	37.0	37.0	37.0	37.0
65-69	35.6619	37.0	37.0	37.0	37.0	37.0
70-74	35.5978	37.0	37.0	37.0	37.0	37.0
75-79	35.557	37.0	37.0	37.0	37.0	37.0
80-84	35.5434	37.0	37.0	37.0	37.0	37.0
85-89	35.301100000000005	37.0	37.0	37.0	32.2	37.0
90-94	35.4595	37.0	37.0	37.0	37.0	37.0
95-99	35.42100000000001	37.0	37.0	37.0	37.0	37.0
100-104	35.2253	37.0	37.0	37.0	29.8	37.0
105-109	35.2816	37.0	37.0	37.0	34.6	37.0
110-114	35.2205	37.0	37.0	37.0	29.8	37.0
115-119	35.0438	37.0	37.0	37.0	27.4	37.0
120-124	34.78189999999999	37.0	37.0	37.0	25.0	37.0
125-129	34.7672	37.0	37.0	37.0	25.0	37.0
130-134	34.7741	37.0	37.0	37.0	25.0	37.0
135-139	34.6716	37.0	37.0	37.0	25.0	37.0
140-144	34.500800000000005	37.0	37.0	37.0	25.0	37.0
145-149	34.4199	37.0	37.0	37.0	25.0	37.0
150	34.257	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	4.0
21	7.0
22	8.0
23	8.0
24	12.0
25	11.0
26	10.0
27	26.0
28	27.0
29	44.0
30	41.0
31	64.0
32	100.0
33	173.0
34	290.0
35	800.0
36	2228.0
37	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	59.099999999999994	10.674999999999999	5.25	24.975
2	19.7	19.35	36.375	24.575
3	19.575	29.025000000000002	25.224999999999998	26.174999999999997
4	8.525	11.225	42.75	37.5
5	10.9	40.725	36.65	11.725
6	33.775	41.699999999999996	16.5	8.025
7	28.1	30.4	21.525	19.975
8	27.525	33.025	20.125	19.325
9	27.400000000000002	11.55	13.325000000000001	47.725
10-14	23.1	22.395	31.900000000000002	22.605
15-19	24.41	27.994999999999997	24.25	23.345
20-24	25.255	24.94	25.324999999999996	24.48
25-29	23.29	25.169999999999998	26.16	25.380000000000003
30-34	24.26	26.72	25.124999999999996	23.895
35-39	23.96	26.1	25.855	24.085
40-44	24.654999999999998	26.51	24.855	23.98
45-49	24.41	25.240000000000002	25.290000000000003	25.06
50-54	24.11	24.705	26.090000000000003	25.095
55-59	23.94	25.215	25.69	25.155
60-64	24.310000000000002	25.240000000000002	25.5	24.95
65-69	24.945	25.169999999999998	25.264999999999997	24.62
70-74	23.544999999999998	25.2	25.905	25.35
75-79	24.795	26.415	24.435000000000002	24.355
80-84	25.4	25.86	24.39	24.349999999999998
85-89	24.205	25.069999999999997	24.740000000000002	25.985000000000003
90-94	24.935	25.285000000000004	25.72	24.060000000000002
95-99	24.73	25.56	25.85	23.86
100-104	24.79	24.465	25.900000000000002	24.845
105-109	24.03	24.915000000000003	25.755	25.3
110-114	25.25	24.865000000000002	24.735	25.15
115-119	24.43	24.595	26.534999999999997	24.44
120-124	24.68	25.590000000000003	25.290000000000003	24.44
125-129	25.705	24.38	25.82	24.095
130-134	25.540000000000003	23.93	25.729999999999997	24.8
135-139	25.53	24.535	25.36	24.575
140-144	25.619999999999997	24.735	25.72	23.925
145-149	25.8	24.41	25.230000000000004	24.560000000000002
150	27.175	26.25	23.125	23.45
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.5
12	0.5
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.5
20	1.0
21	0.5
22	0.5
23	4.5
24	6.0
25	4.5
26	15.0
27	24.0
28	23.0
29	23.5
30	22.0
31	18.0
32	23.5
33	45.5
34	61.5
35	62.0
36	69.5
37	72.0
38	79.0
39	82.0
40	91.0
41	114.5
42	104.0
43	86.0
44	88.5
45	96.5
46	106.0
47	112.5
48	109.0
49	152.5
50	171.5
51	151.5
52	195.5
53	238.0
54	242.5
55	224.0
56	207.0
57	162.0
58	121.5
59	138.5
60	126.5
61	93.0
62	60.5
63	38.5
64	37.0
65	28.5
66	17.0
67	13.5
68	10.5
69	5.5
70	3.5
71	1.5
72	2.5
73	2.0
74	0.5
75	0.5
76	1.0
77	0.5
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15557246101179	52.025
2	11.905667554203118	15.65
3	3.6135412704450367	7.124999999999999
4	1.5595283377710156	4.1000000000000005
5	0.798782807151008	2.625
6	0.7607455306200076	3.0
7	0.5325218714340053	2.45
8	0.4564473183720046	2.4
9	0.34233548877900344	2.025
>10	0.8748573602130088	8.6
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	28	0.7000000000000001	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	24	0.6	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	23	0.575	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	20	0.5	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	19	0.475	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	18	0.44999999999999996	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	17	0.42500000000000004	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	17	0.42500000000000004	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	15	0.375	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	15	0.375	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	14	0.35000000000000003	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	13	0.325	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	13	0.325	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	13	0.325	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	12	0.3	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	11	0.27499999999999997	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	11	0.27499999999999997	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	11	0.27499999999999997	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	10	0.25	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	10	0.25	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	10	0.25	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	10	0.25	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	10	0.25	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	9	0.22499999999999998	No Hit
GTACATGGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCT	9	0.22499999999999998	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	9	0.22499999999999998	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	9	0.22499999999999998	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	9	0.22499999999999998	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	9	0.22499999999999998	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	9	0.22499999999999998	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	9	0.22499999999999998	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	9	0.22499999999999998	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	8	0.2	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	8	0.2	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	8	0.2	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	8	0.2	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	8	0.2	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	8	0.2	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	8	0.2	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	8	0.2	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	8	0.2	No Hit
GGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCCAATGCTGCAG	8	0.2	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	8	0.2	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	8	0.2	No Hit
CTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTGTCCATCTGC	7	0.17500000000000002	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	7	0.17500000000000002	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	7	0.17500000000000002	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	7	0.17500000000000002	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	7	0.17500000000000002	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	7	0.17500000000000002	No Hit
CCATTAGAGAGATGTGTACCAGAGCCGGTGGCCACATTGCTGCCCCGAGG	7	0.17500000000000002	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	7	0.17500000000000002	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	7	0.17500000000000002	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	7	0.17500000000000002	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	7	0.17500000000000002	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	7	0.17500000000000002	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	7	0.17500000000000002	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	7	0.17500000000000002	No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG	6	0.15	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	6	0.15	No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT	6	0.15	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	6	0.15	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	6	0.15	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	6	0.15	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	6	0.15	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	6	0.15	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	6	0.15	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	6	0.15	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	6	0.15	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	6	0.15	No Hit
ATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCC	6	0.15	No Hit
ACCAGACTCTGATTCCATGAGGAGGGCCTCAAGGACTTGAAGAAATCCTG	6	0.15	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	6	0.15	No Hit
GTACATGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACAT	6	0.15	No Hit
CCCCTGACCAGCAGAGGCTGATCTTTGCCGGCAAGCAGCTGGAAGATGGC	6	0.15	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	6	0.15	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	6	0.15	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	6	0.15	No Hit
GTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGGTTACCTAAAT	5	0.125	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	5	0.125	No Hit
GGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTA	5	0.125	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	5	0.125	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	5	0.125	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	5	0.125	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	5	0.125	No Hit
ACCCTGCACCTGGTCCTTCGCCTGAGAGGTGGCATGCAGATCTTCGTGAA	5	0.125	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	5	0.125	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	5	0.125	No Hit
GTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATT	5	0.125	No Hit
ACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACCG	5	0.125	No Hit
GTCCATCTGCTGCAGCTCAGCTTCTTCGGACATCTGCCCAGGATTTCTTC	5	0.125	No Hit
GGTTAATAGTGTAATTGAATTATAGTGAAATCATATTACTAGACCTGATG	5	0.125	No Hit
GTGCAGGGTTGACTCCTTCTGGATGTTGTAATCAGAGAGGGTGCGGCCAT	5	0.125	No Hit
GTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTAGCGAAAGCCTCA	5	0.125	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	5	0.125	No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA	5	0.125	No Hit
GGGCAGCTCTAAGAAAGACAGGTCACGGGTTCCCTGATAAGTTTCTGAGT	5	0.125	No Hit
CTCCACACCCACCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTT	5	0.125	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.05	0.0
30-31	0.0	0.0	0.0	0.05	0.0
32-33	0.0	0.0	0.0	0.05	0.0
34-35	0.0	0.0	0.0	0.075	0.0
36-37	0.0	0.0	0.0	0.125	0.0
38-39	0.0	0.0	0.0	0.25	0.0
40-41	0.0	0.0	0.0	0.425	0.0
42-43	0.0	0.0	0.0	0.5875	0.0
44-45	0.0	0.0	0.0	0.7875	0.0
46-47	0.0	0.0	0.0	1.0125	0.0
48-49	0.0	0.0	0.0	1.125	0.0
50-51	0.0	0.0	0.0	1.25	0.0
52-53	0.0	0.0	0.0	1.4874999999999998	0.0
54-55	0.0	0.0	0.0	1.6375	0.0
56-57	0.0	0.0	0.0	1.7999999999999998	0.0
58-59	0.0	0.0	0.0	1.9625	0.0
60-61	0.0	0.0	0.0	2.1125	0.0
62-63	0.0	0.0	0.0	2.3375	0.0
64-65	0.0	0.0	0.0	2.4875	0.0
66-67	0.0	0.0	0.0	2.725	0.0
68-69	0.0	0.0	0.0	2.975	0.0
70-71	0.0	0.0	0.0	3.2	0.0
72-73	0.0	0.0	0.0	3.55	0.0
74-75	0.0	0.0	0.0	3.7625	0.0
76-77	0.0	0.0	0.0	3.9625000000000004	0.0
78-79	0.0	0.0	0.0	4.075	0.0
80-81	0.0	0.0	0.0	4.2875	0.0
82-83	0.0	0.0	0.0	4.5875	0.0
84-85	0.0	0.0	0.0	4.85	0.0
86-87	0.0	0.0	0.0	5.15	0.0
88-89	0.0	0.0	0.0	5.3125	0.0
90-91	0.0	0.0	0.0	5.5375	0.0
92-93	0.0	0.0	0.0	5.775	0.0
94-95	0.0	0.0	0.0	6.0625	0.0
96-97	0.0	0.0	0.0	6.3875	0.0
98-99	0.0	0.0	0.0	6.574999999999999	0.0
100-101	0.0	0.0	0.0	6.9125	0.0
102-103	0.0	0.0	0.0	7.1875	0.0
104-105	0.0	0.0	0.0	7.4375	0.0
106-107	0.0	0.0	0.0	7.775	0.0
108-109	0.0	0.0	0.0	8.125	0.0
110-111	0.0	0.0	0.0	8.375	0.0
112-113	0.0	0.0	0.0	8.875	0.0
114-115	0.0	0.0	0.0	9.45	0.0
116-117	0.0	0.0	0.0	10.0125	0.0
118-119	0.0	0.0	0.0	10.45	0.0
120-121	0.0	0.0	0.0	10.925	0.0
122-123	0.0	0.0	0.0	11.4	0.0
124-125	0.0	0.0	0.0	11.9875	0.0
126-127	0.0	0.0	0.0	12.5	0.0
128-129	0.0	0.0	0.0	12.9625	0.0
130-131	0.0	0.0	0.0	13.5625	0.0
132-133	0.0	0.0	0.0	14.1375	0.0
134-135	0.0	0.0	0.0	14.6875	0.0
136-137	0.0	0.0	0.0	15.5375	0.0
138	0.0	0.0	0.0	15.95	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTTGT	10	0.006973645	144.0	9
ATGGGGA	10	0.006973645	144.0	5
CTTTATT	15	1.1730364E-4	144.0	2
TAAACAG	10	0.006973645	144.0	5
TTATCCT	10	0.006973645	144.0	5
CCAGAGA	10	0.006973645	144.0	4
TGTTTAT	10	0.006973645	144.0	2
TCCAGAG	10	0.006973645	144.0	3
GAGCATC	10	0.006973645	144.0	9
GTTTATC	10	0.006973645	144.0	3
CAGAGAG	10	0.006973645	144.0	5
TATCCTG	10	0.006973645	144.0	6
AGAGCAT	10	0.006973645	144.0	8
AGAGAGC	10	0.006973645	144.0	6
ATGTTTA	10	0.006973645	144.0	1
CATGGGG	15	1.1730364E-4	144.0	4
TCCTGGT	10	0.006973645	144.0	8
TTTATCC	10	0.006973645	144.0	4
GCTTTAT	10	0.006973645	144.0	1
ACATGGG	25	8.956223E-4	86.399994	3
>>END_MODULE
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
Read 2413530 spots for SRR31990342.sra
Written 2413530 spots for SRR31990342.sra
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
Read 2413529 spots for SRR31990342.sra
Written 2413529 spots for SRR31990342.sra
SRR ids: ['SRR31990342.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5c6yljh_
SRR31990342.sra spots: 19308233
blocks: [[1, 2413529], [2413530, 4827058], [4827059, 7240587], [7240588, 9654116], [9654117, 12067645], [12067646, 14481174], [14481175, 16894703], [16894704, 19308233]]
SRR31990342 file size 7068908
SRR31990342 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990342 SRR31990342_1.fastq SRR31990342_2.fastq
Input file:	SRR31990342_1.fastq
Paired file:	SRR31990342_2.fastq
trimmed:	SRR31990342-trimmed-pair1.fastq, SRR31990342-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:45:40 2026 >> started

Sat Apr 25 18:46:04 2026 >> done (23.572s)
19308233 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
19308233 (100.00%) read pairs available; of these:
    1216 ( 0.01%) trimmed read pairs available after processing
19307017 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
138	       5	  0.00%
139	       0	  0.00%
140	       0	  0.00%
141	       0	  0.00%
142	       0	  0.00%
143	       0	  0.00%
144	       0	  0.00%
145	       3	  0.00%
146	       3	  0.00%
147	      42	  0.00%
148	      28	  0.00%
149	    1135	  0.01%
150	19307017	 99.99%
19308233 reads passed initial QC


criterion=sequence-density
sequence-density=14.61
sequence-density-rank=1
fanout-score=34.42
fanout-score-rank=2
prefix-density=15.64
prefix-fanout=32.2
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=15
fanout-score=319.11
fanout-score-rank=1
prefix-density=3.02
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=14.76
sequence-density-rank=1
fanout-score=34.17
fanout-score-rank=2
prefix-density=15.80
prefix-fanout=31.9
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=14
fanout-score=244.99
fanout-score-rank=1
prefix-density=2.99
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA -o SRR31990342 SRR31990342_1.fastq SRR31990342_2.fastq
Input file:	SRR31990342_1.fastq
Paired file:	SRR31990342_2.fastq
trimmed:	SRR31990342-trimmed-pair1.fastq, SRR31990342-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 18:47:20 2026 >> started

Sat Apr 25 18:48:03 2026 >> done (43.680s)
16733802 read pairs processed; of these:
      53 ( 0.00%) short read pairs filtered out after trimming by size control
       6 ( 0.00%) empty read pairs filtered out after trimming by size control
16733743 (100.00%) read pairs available; of these:
 3397022 (20.30%) trimmed read pairs available after processing
13336721 (79.70%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       6	  0.00%
 19	      12	  0.00%
 20	      14	  0.00%
 21	      23	  0.00%
 22	      31	  0.00%
 23	      49	  0.00%
 24	      32	  0.00%
 25	     115	  0.00%
 26	      79	  0.00%
 27	     187	  0.00%
 28	     510	  0.00%
 29	     573	  0.00%
 30	     834	  0.00%
 31	    1503	  0.01%
 32	    1408	  0.01%
 33	    2381	  0.01%
 34	    2004	  0.01%
 35	    3074	  0.02%
 36	    5688	  0.03%
 37	    7849	  0.05%
 38	    9647	  0.06%
 39	    9356	  0.06%
 40	   11060	  0.07%
 41	   10169	  0.06%
 42	   10260	  0.06%
 43	   10246	  0.06%
 44	   14564	  0.09%
 45	   12788	  0.08%
 46	   17416	  0.10%
 47	   15451	  0.09%
 48	   18248	  0.11%
 49	   14141	  0.08%
 50	   15731	  0.09%
 51	   13490	  0.08%
 52	   13372	  0.08%
 53	   14090	  0.08%
 54	   13947	  0.08%
 55	   22872	  0.14%
 56	   16623	  0.10%
 57	   15385	  0.09%
 58	   14353	  0.09%
 59	   13692	  0.08%
 60	   15930	  0.10%
 61	   15191	  0.09%
 62	   18355	  0.11%
 63	   19374	  0.12%
 64	   15277	  0.09%
 65	   14251	  0.09%
 66	   21412	  0.13%
 67	   19365	  0.12%
 68	   17450	  0.10%
 69	   24687	  0.15%
 70	   19607	  0.12%
 71	   22372	  0.13%
 72	   20142	  0.12%
 73	   19067	  0.11%
 74	   19946	  0.12%
 75	   21778	  0.13%
 76	   19919	  0.12%
 77	   16935	  0.10%
 78	   18346	  0.11%
 79	   17182	  0.10%
 80	   24567	  0.15%
 81	   34630	  0.21%
 82	   28122	  0.17%
 83	   17928	  0.11%
 84	   18865	  0.11%
 85	   21451	  0.13%
 86	   24010	  0.14%
 87	   27706	  0.17%
 88	   23987	  0.14%
 89	   27863	  0.17%
 90	   24623	  0.15%
 91	   22562	  0.13%
 92	   24356	  0.15%
 93	   28559	  0.17%
 94	   25747	  0.15%
 95	   32252	  0.19%
 96	   29250	  0.17%
 97	   23651	  0.14%
 98	   33756	  0.20%
 99	   32987	  0.20%
100	   29730	  0.18%
101	   29396	  0.18%
102	   27020	  0.16%
103	   33679	  0.20%
104	   34164	  0.20%
105	   38236	  0.23%
106	   34925	  0.21%
107	   36197	  0.22%
108	   38002	  0.23%
109	   35032	  0.21%
110	   32527	  0.19%
111	   35894	  0.21%
112	   35736	  0.21%
113	   36278	  0.22%
114	   36073	  0.22%
115	   30325	  0.18%
116	   30343	  0.18%
117	   32778	  0.20%
118	   29440	  0.18%
119	   39031	  0.23%
120	   38229	  0.23%
121	   41342	  0.25%
122	   40510	  0.24%
123	   44962	  0.27%
124	   39568	  0.24%
125	   41402	  0.25%
126	   43615	  0.26%
127	   43070	  0.26%
128	   34879	  0.21%
129	   45266	  0.27%
130	   53203	  0.32%
131	   45982	  0.27%
132	   47030	  0.28%
133	   54197	  0.32%
134	   45523	  0.27%
135	   58522	  0.35%
136	   51836	  0.31%
137	   41831	  0.25%
138	   48683	  0.29%
139	   50516	  0.30%
140	   58380	  0.35%
141	   54371	  0.32%
142	   55924	  0.33%
143	   52457	  0.31%
144	   57118	  0.34%
145	   45825	  0.27%
146	   56489	  0.34%
147	   60389	  0.36%
148	   66725	  0.40%
149	   68694	  0.41%
150	13335668	 79.69%


criterion=sequence-density
sequence-density=2.13
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=29
prefix-density=2.17
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=312.02
fanout-score-rank=1
prefix-density=2.99
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=2.16
sequence-density-rank=1
fanout-score=2.02
fanout-score-rank=27
prefix-density=2.18
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=30
fanout-score=265.03
fanout-score-rank=1
prefix-density=2.93
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990342 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 18:49:40
                             Started mapping on |	Apr 25 18:49:40
                                    Finished on |	Apr 25 18:52:06
       Mapping speed, Million of reads per hour |	476.09

                          Number of input reads |	19308174
                      Average input read length |	277
                                    UNIQUE READS:
                   Uniquely mapped reads number |	14252796
                        Uniquely mapped reads % |	73.82%
                          Average mapped length |	282.55
                       Number of splices: Total |	14132731
            Number of splices: Annotated (sjdb) |	14051534
                       Number of splices: GT/AG |	14073367
                       Number of splices: GC/AG |	22053
                       Number of splices: AT/AC |	3404
               Number of splices: Non-canonical |	33907
                      Mismatch rate per base, % |	0.26%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.46
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1728971
             % of reads mapped to multiple loci |	8.95%
        Number of reads mapped to too many loci |	38541
             % of reads mapped to too many loci |	0.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.93%
                     % of reads unmapped: other |	0.10%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3326407	3326407	3326407
N_multimapping	1728971	1728971	1728971
N_noFeature	1227031	7370320	7247729
N_ambiguous	1216541	187444	190832
UnstrandedReadsAssigned:11809224 PositiveStrandReadsAssigned:6695032 NegativeStrandReadsAssigned:6814235
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=149 echo kmer=145
SRR31990342 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990342-trimmed-pair1.fastq
                             SRR31990342-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 19,308,174 reads, 16,105,802 reads pseudoaligned
[quant] estimated average fragment length: 210.343
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,032 rounds

 109283 SRR31990342.ke.tsv
  52636 SRR31990342.se.tsv
 161919 total
==> SRR31990342.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR31990342.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	3
ENSMUSG00000103025	4
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990342 completed mapping pipeline successfully
