Starting /dee2/code/volunteer_pipeline.sh SRR31990347
    current disk space = 45461032
    free memory = 202243124 
SRR31990347 SRAfilesize
2461db9ff8c5f9968f8a4db7a5ebe7e9  SRR31990347.sra
SRR31990347.sra file validated
SRR31990347 is paired end
SRR31990347 is conventional basespace
SRR31990347 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.048	37.0	37.0	37.0	37.0	37.0
2	36.1165	37.0	37.0	37.0	37.0	37.0
3	36.22	37.0	37.0	37.0	37.0	37.0
4	36.236	37.0	37.0	37.0	37.0	37.0
5	36.35	37.0	37.0	37.0	37.0	37.0
6	36.4265	37.0	37.0	37.0	37.0	37.0
7	36.34	37.0	37.0	37.0	37.0	37.0
8	36.2645	37.0	37.0	37.0	37.0	37.0
9	36.3515	37.0	37.0	37.0	37.0	37.0
10-14	36.2581	37.0	37.0	37.0	37.0	37.0
15-19	36.3104	37.0	37.0	37.0	37.0	37.0
20-24	36.2021	37.0	37.0	37.0	37.0	37.0
25-29	36.1074	37.0	37.0	37.0	37.0	37.0
30-34	36.068799999999996	37.0	37.0	37.0	37.0	37.0
35-39	36.033899999999996	37.0	37.0	37.0	37.0	37.0
40-44	35.953	37.0	37.0	37.0	37.0	37.0
45-49	35.7981	37.0	37.0	37.0	37.0	37.0
50-54	35.76649999999999	37.0	37.0	37.0	37.0	37.0
55-59	35.765	37.0	37.0	37.0	37.0	37.0
60-64	35.7618	37.0	37.0	37.0	37.0	37.0
65-69	35.7244	37.0	37.0	37.0	37.0	37.0
70-74	35.6632	37.0	37.0	37.0	37.0	37.0
75-79	35.6121	37.0	37.0	37.0	37.0	37.0
80-84	35.6418	37.0	37.0	37.0	37.0	37.0
85-89	35.4989	37.0	37.0	37.0	37.0	37.0
90-94	35.346700000000006	37.0	37.0	37.0	37.0	37.0
95-99	35.318799999999996	37.0	37.0	37.0	37.0	37.0
100-104	35.1854	37.0	37.0	37.0	34.6	37.0
105-109	34.95	37.0	37.0	37.0	25.0	37.0
110-114	34.9521	37.0	37.0	37.0	25.0	37.0
115-119	34.823499999999996	37.0	37.0	37.0	25.0	37.0
120-124	34.7495	37.0	37.0	37.0	25.0	37.0
125-129	34.4187	37.0	37.0	37.0	25.0	37.0
130-134	34.4543	37.0	37.0	37.0	25.0	37.0
135-139	34.2871	37.0	37.0	37.0	25.0	37.0
140-144	34.14	37.0	37.0	37.0	25.0	37.0
145-149	33.9858	37.0	37.0	37.0	25.0	37.0
150	34.184	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	7.0
22	11.0
23	5.0
24	7.0
25	10.0
26	16.0
27	32.0
28	26.0
29	44.0
30	62.0
31	96.0
32	132.0
33	200.0
34	261.0
35	568.0
36	2403.0
37	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	59.23423423423423	10.71071071071071	4.404404404404405	25.650650650650654
2	19.675	18.525	37.25	24.55
3	18.625	28.95	24.425	28.000000000000004
4	8.25	9.049999999999999	43.6	39.1
5	10.7	41.199999999999996	36.425000000000004	11.675
6	32.15	42.925000000000004	15.4	9.525
7	27.200000000000003	31.225	23.0	18.575
8	25.974999999999998	34.575	18.9	20.549999999999997
9	25.174999999999997	11.1	14.025000000000002	49.7
10-14	21.9	22.575	32.33	23.195
15-19	23.315	28.89	24.759999999999998	23.035
20-24	24.51	25.88	24.845	24.765
25-29	22.96	24.945	26.83	25.264999999999997
30-34	23.119999999999997	26.575	25.915	24.39
35-39	23.375	26.040000000000003	26.69	23.895
40-44	23.369999999999997	26.924999999999997	25.759999999999998	23.945
45-49	24.435000000000002	24.33	26.14	25.095
50-54	24.13	25.055	26.77	24.044999999999998
55-59	23.93	25.83	26.13	24.11
60-64	23.974999999999998	25.735000000000003	26.534999999999997	23.755000000000003
65-69	23.580000000000002	26.0	26.32	24.099999999999998
70-74	22.91	24.725	26.810000000000002	25.555
75-79	24.33	26.215	25.215	24.240000000000002
80-84	24.03	26.040000000000003	25.124999999999996	24.805
85-89	23.330000000000002	25.14	25.055	26.474999999999998
90-94	23.515	26.265	26.3	23.919999999999998
95-99	23.849999999999998	25.69	26.58	23.880000000000003
100-104	24.36	25.615	25.564999999999998	24.46
105-109	23.155	25.21	26.16	25.474999999999998
110-114	23.965	25.919999999999998	25.374999999999996	24.740000000000002
115-119	23.095	24.905	26.97	25.03
120-124	23.21	25.89	26.185000000000002	24.715
125-129	24.415	24.535	26.279999999999998	24.77
130-134	25.474999999999998	23.244999999999997	25.665	25.615
135-139	24.38	25.230000000000004	25.540000000000003	24.85
140-144	25.319999999999997	24.58	26.035000000000004	24.065
145-149	25.445	24.015	24.815	25.724999999999998
150	25.825	25.474999999999998	25.025	23.674999999999997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	0.0
17	1.0
18	1.5
19	1.0
20	0.5
21	2.5
22	5.0
23	5.0
24	4.0
25	5.0
26	20.5
27	28.5
28	34.0
29	34.0
30	26.0
31	28.5
32	31.5
33	49.0
34	61.0
35	60.0
36	71.0
37	76.0
38	86.0
39	97.0
40	107.5
41	117.0
42	96.0
43	79.5
44	104.0
45	106.5
46	109.0
47	131.0
48	120.0
49	145.5
50	156.0
51	138.5
52	188.0
53	232.0
54	225.0
55	209.0
56	203.0
57	167.0
58	129.5
59	126.5
60	114.0
61	88.5
62	52.5
63	29.5
64	23.5
65	23.0
66	17.0
67	7.0
68	5.5
69	6.0
70	3.0
71	0.5
72	1.0
73	1.0
74	0.5
75	1.5
76	1.5
77	0.5
78	0.0
79	1.0
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.275000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21770051363097	50.125
2	11.339391544843934	14.35
3	3.7139470564994075	7.049999999999999
4	1.4223627024891348	3.5999999999999996
5	1.3038324772817067	4.125
6	0.5926511260371394	2.25
7	0.39510075069142636	1.7500000000000002
8	0.2765705254839984	1.4000000000000001
9	0.316080600553141	1.7999999999999998
>10	1.4223627024891348	13.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	37	0.9249999999999999	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	25	0.625	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	25	0.625	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	23	0.575	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	22	0.5499999999999999	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	21	0.525	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	20	0.5	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	18	0.44999999999999996	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	18	0.44999999999999996	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	17	0.42500000000000004	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	17	0.42500000000000004	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	16	0.4	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	16	0.4	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	16	0.4	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	14	0.35000000000000003	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	13	0.325	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	13	0.325	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	13	0.325	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	13	0.325	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	12	0.3	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	12	0.3	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	12	0.3	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	12	0.3	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	12	0.3	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	11	0.27499999999999997	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	11	0.27499999999999997	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	11	0.27499999999999997	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	11	0.27499999999999997	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	11	0.27499999999999997	No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG	10	0.25	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	10	0.25	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	10	0.25	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	10	0.25	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	10	0.25	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	10	0.25	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	10	0.25	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	9	0.22499999999999998	No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT	9	0.22499999999999998	No Hit
CAGCTGAAGAGACTGGTGGATACCCTCCCACAAGAGACCAGGATAAACAT	9	0.22499999999999998	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	9	0.22499999999999998	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	9	0.22499999999999998	No Hit
CTATAAGCCAGCTCCAGGAACCTACTGCTACATCATGAAGATGGCTCCAG	9	0.22499999999999998	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	9	0.22499999999999998	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	9	0.22499999999999998	No Hit
GCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTCA	8	0.2	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	8	0.2	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	8	0.2	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	8	0.2	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	8	0.2	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	8	0.2	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	8	0.2	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC	7	0.17500000000000002	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	7	0.17500000000000002	No Hit
GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT	7	0.17500000000000002	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	7	0.17500000000000002	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	7	0.17500000000000002	No Hit
GACCAGGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTC	7	0.17500000000000002	No Hit
GGGTCTTTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGAT	7	0.17500000000000002	No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT	7	0.17500000000000002	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	7	0.17500000000000002	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	7	0.17500000000000002	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	6	0.15	No Hit
CTCTACAACTTTACTGTTTTAAATGCCACTTAGATTCTATTTAGGTAACC	6	0.15	No Hit
GAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGA	6	0.15	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	6	0.15	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	6	0.15	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	6	0.15	No Hit
ACTCAGACCTGGTCAGCCTTGCCAAAGAGTGTCCCGGAATAGAGCCCGTG	6	0.15	No Hit
GGTCTGAGCCAGGGTTGAAAGGCTTCAGGGATGCCACATAACCAGACTCT	6	0.15	No Hit
GCTTAGAGGTGGGTGTGGAGGGCTTGGCCTGGAAGTTCTGGAGTTTTCTA	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
CCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGCATCCCTGA	6	0.15	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	6	0.15	No Hit
GCCTAACACTTCCATCTTCCCTGTTTCCAACTCATGCCTTCAAACGTACA	6	0.15	No Hit
GTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCCCTACAATCACCACG	6	0.15	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	6	0.15	No Hit
GTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGGTTACCTAAAT	5	0.125	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	5	0.125	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	5	0.125	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	5	0.125	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	5	0.125	No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC	5	0.125	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	5	0.125	No Hit
GTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCTAGCCCC	5	0.125	No Hit
GACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCTGCTTGTCTGACAAAG	5	0.125	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	5	0.125	No Hit
AGATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCT	5	0.125	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	5	0.125	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	5	0.125	No Hit
CTACAAGGCCGACCTTCAAACTAGAACCTTTTAGAACTTCACAAAACCTC	5	0.125	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	5	0.125	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	5	0.125	No Hit
CTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGC	5	0.125	No Hit
CTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTG	5	0.125	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	5	0.125	No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	5	0.125	No Hit
CCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTGTCCATC	5	0.125	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	5	0.125	No Hit
GCAGGAGACTCAGCCCGAGGAAATCGCAGATAAGTTTTTAATTAAAAAGA	5	0.125	No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG	5	0.125	No Hit
CATTACAACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTAC	5	0.125	No Hit
GTTCTAATTCTTTTTACTGCTCAATCTTTTTAATTAAAAACTTATCTGCG	5	0.125	No Hit
ATCCTGGGCAGATGTCCGAAGAAGCTGAGCTGCAGCAGATGGACAGCATG	5	0.125	No Hit
CCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGG	5	0.125	No Hit
GGATCCCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACC	5	0.125	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	5	0.125	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	5	0.125	No Hit
GGCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTC	5	0.125	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.05	0.0
28-29	0.0	0.0	0.0	0.05	0.0
30-31	0.0	0.0	0.0	0.1	0.0
32-33	0.0	0.0	0.0	0.125	0.0
34-35	0.0	0.0	0.0	0.15	0.0
36-37	0.0	0.0	0.0	0.16249999999999998	0.0
38-39	0.0	0.0	0.0	0.225	0.0
40-41	0.0	0.0	0.0	0.2625	0.0
42-43	0.0	0.0	0.0	0.375	0.0
44-45	0.0	0.0	0.0	0.45	0.0
46-47	0.0	0.0	0.0	0.6	0.0
48-49	0.0	0.0	0.0	0.7375	0.0
50-51	0.0	0.0	0.0	0.9375	0.0
52-53	0.0	0.0	0.0	1.075	0.0
54-55	0.0	0.0	0.0	1.1	0.0
56-57	0.0	0.0	0.0	1.25	0.0
58-59	0.0	0.0	0.0	1.475	0.0
60-61	0.0	0.0	0.0	1.6625	0.0
62-63	0.0	0.0	0.0	1.825	0.0
64-65	0.0	0.0	0.0	2.05	0.0
66-67	0.0	0.0	0.0	2.1875	0.0
68-69	0.0	0.0	0.0	2.4875	0.0
70-71	0.0	0.0	0.0	2.7625	0.0
72-73	0.0	0.0	0.0	3.025	0.0
74-75	0.0	0.0	0.0	3.1875	0.0
76-77	0.0	0.0	0.0	3.4000000000000004	0.0
78-79	0.0	0.0	0.0	3.625	0.0
80-81	0.0	0.0	0.0	3.8875	0.0
82-83	0.0	0.0	0.0	4.3625	0.0
84-85	0.0	0.0	0.0	4.625	0.0
86-87	0.0	0.0	0.0	4.9	0.0
88-89	0.0	0.0	0.0	5.1	0.0
90-91	0.0	0.0	0.0	5.3125	0.0
92-93	0.0	0.0	0.0	5.5375	0.0
94-95	0.0	0.0	0.0	5.9125	0.0
96-97	0.0	0.0	0.0	6.2875	0.0
98-99	0.0	0.0	0.0	6.75	0.0
100-101	0.0	0.0	0.0	7.15	0.0
102-103	0.0	0.0	0.0	7.5875	0.0
104-105	0.0	0.0	0.0	7.9375	0.0
106-107	0.0	0.0	0.0	8.225000000000001	0.0
108-109	0.0	0.0	0.0	8.6625	0.0
110-111	0.0	0.0	0.0	9.2375	0.0
112-113	0.0	0.0	0.0	9.9	0.0
114-115	0.0	0.0	0.0	10.462499999999999	0.0
116-117	0.0	0.0	0.0	10.825	0.0
118-119	0.0	0.0	0.0	11.1875	0.0
120-121	0.0	0.0	0.0	11.725	0.0
122-123	0.0	0.0	0.0	12.225000000000001	0.0
124-125	0.0	0.0	0.0	12.725000000000001	0.0
126-127	0.0	0.0	0.0	13.412500000000001	0.0
128-129	0.0	0.0	0.0	14.125	0.0
130-131	0.0	0.0	0.0	14.8125	0.0
132-133	0.0	0.0	0.0	15.675	0.0
134-135	0.0	0.0	0.0	16.3	0.0
136-137	0.0	0.0	0.0	17.0375	0.0
138	0.0	0.0	0.0	17.5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGACC	10	0.006973645	144.0	8
CAAGAGT	10	0.006973645	144.0	4
TATAGGA	10	0.006973645	144.0	4
GGGGGGG	295	8.751926E-6	7.322034	140-144
>>END_MODULE
SRR31990347 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990347_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.8745	37.0	37.0	37.0	25.0	37.0
2	36.1415	37.0	37.0	37.0	37.0	37.0
3	36.3395	37.0	37.0	37.0	37.0	37.0
4	36.1655	37.0	37.0	37.0	37.0	37.0
5	36.2025	37.0	37.0	37.0	37.0	37.0
6	36.29	37.0	37.0	37.0	37.0	37.0
7	36.248	37.0	37.0	37.0	37.0	37.0
8	36.3775	37.0	37.0	37.0	37.0	37.0
9	36.313	37.0	37.0	37.0	37.0	37.0
10-14	36.312200000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.296	37.0	37.0	37.0	37.0	37.0
20-24	36.2424	37.0	37.0	37.0	37.0	37.0
25-29	36.2749	37.0	37.0	37.0	37.0	37.0
30-34	36.2153	37.0	37.0	37.0	37.0	37.0
35-39	36.1921	37.0	37.0	37.0	37.0	37.0
40-44	36.143899999999995	37.0	37.0	37.0	37.0	37.0
45-49	35.961200000000005	37.0	37.0	37.0	37.0	37.0
50-54	36.056200000000004	37.0	37.0	37.0	37.0	37.0
55-59	35.9283	37.0	37.0	37.0	37.0	37.0
60-64	35.910199999999996	37.0	37.0	37.0	37.0	37.0
65-69	35.829600000000006	37.0	37.0	37.0	37.0	37.0
70-74	35.8639	37.0	37.0	37.0	37.0	37.0
75-79	35.77810000000001	37.0	37.0	37.0	37.0	37.0
80-84	35.807100000000005	37.0	37.0	37.0	37.0	37.0
85-89	35.666	37.0	37.0	37.0	37.0	37.0
90-94	35.6993	37.0	37.0	37.0	37.0	37.0
95-99	35.5974	37.0	37.0	37.0	37.0	37.0
100-104	35.4742	37.0	37.0	37.0	37.0	37.0
105-109	35.505399999999995	37.0	37.0	37.0	37.0	37.0
110-114	35.4292	37.0	37.0	37.0	37.0	37.0
115-119	35.3437	37.0	37.0	37.0	34.6	37.0
120-124	35.1737	37.0	37.0	37.0	29.8	37.0
125-129	35.0877	37.0	37.0	37.0	27.4	37.0
130-134	35.0087	37.0	37.0	37.0	25.0	37.0
135-139	34.923700000000004	37.0	37.0	37.0	25.0	37.0
140-144	34.736000000000004	37.0	37.0	37.0	25.0	37.0
145-149	34.7079	37.0	37.0	37.0	25.0	37.0
150	34.6345	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	0.0
19	0.0
20	1.0
21	9.0
22	14.0
23	6.0
24	9.0
25	4.0
26	11.0
27	15.0
28	24.0
29	27.0
30	45.0
31	44.0
32	75.0
33	126.0
34	230.0
35	673.0
36	2468.0
37	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	60.224999999999994	11.075	4.55	24.15
2	20.8	18.775	37.625	22.8
3	20.7	27.950000000000003	25.1	26.25
4	8.924999999999999	10.025	44.4	36.65
5	10.174999999999999	40.525	37.75	11.55
6	31.974999999999998	43.95	15.75	8.325000000000001
7	27.775	30.675	22.425	19.125
8	27.075	34.725	19.900000000000002	18.3
9	27.125	11.175	13.3	48.4
10-14	22.91	22.205	32.135000000000005	22.75
15-19	23.21	28.845	25.25	22.695
20-24	25.330000000000002	25.345000000000002	25.21	24.115000000000002
25-29	22.775000000000002	25.374999999999996	26.240000000000002	25.61
30-34	24.295	26.305	25.395	24.005000000000003
35-39	23.41	26.834999999999997	25.985000000000003	23.77
40-44	24.32	27.794999999999998	24.365000000000002	23.52
45-49	24.29	24.82	25.88	25.009999999999998
50-54	23.98	25.679999999999996	27.025	23.315
55-59	24.005000000000003	25.95	25.580000000000002	24.465
60-64	23.96	26.455000000000002	25.779999999999998	23.805
65-69	24.46	25.64	25.869999999999997	24.03
70-74	23.885	25.195	26.68	24.240000000000002
75-79	24.95	25.785000000000004	25.71	23.555
80-84	24.349999999999998	26.724999999999998	24.345	24.58
85-89	23.794999999999998	25.205	25.679999999999996	25.319999999999997
90-94	24.8	25.44	26.195	23.565
95-99	23.455000000000002	25.825	27.04	23.68
100-104	24.54	25.145	26.21	24.104999999999997
105-109	22.665	25.480000000000004	26.38	25.474999999999998
110-114	24.33	25.314999999999998	25.415	24.94
115-119	23.674999999999997	24.740000000000002	26.955000000000002	24.63
120-124	24.095	25.424999999999997	26.235000000000003	24.245
125-129	25.39	25.105	25.8	23.705000000000002
130-134	24.485	23.669999999999998	26.375	25.47
135-139	24.48	24.59	26.075	24.855
140-144	24.25	24.25	27.35	24.15
145-149	24.055	23.72	26.85	25.374999999999996
150	26.3	25.5	25.2	23.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	1.0
13	1.0
14	1.0
15	3.0
16	2.0
17	0.5
18	0.5
19	0.0
20	1.0
21	2.0
22	3.0
23	4.5
24	5.5
25	7.0
26	14.5
27	29.0
28	30.0
29	24.5
30	26.0
31	27.5
32	32.5
33	47.0
34	59.5
35	61.0
36	69.5
37	75.0
38	88.0
39	91.0
40	105.0
41	125.0
42	105.0
43	90.0
44	102.5
45	109.0
46	113.0
47	114.5
48	111.0
49	153.5
50	159.5
51	141.0
52	203.0
53	240.5
54	228.0
55	210.0
56	191.5
57	155.0
58	129.5
59	140.5
60	119.5
61	83.5
62	49.5
63	28.0
64	26.5
65	22.5
66	13.0
67	7.0
68	2.5
69	2.0
70	4.0
71	2.0
72	1.0
73	1.0
74	0.0
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18660287081339	49.65
2	11.562998405103668	14.499999999999998
3	3.269537480063796	6.15
4	1.5948963317384368	4.0
5	0.9569377990430622	3.0
6	0.6379585326953748	2.4
7	0.5980861244019139	2.625
8	0.3987240829346092	2.0
9	0.27910685805422647	1.575
>10	1.5151515151515151	14.099999999999998
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	41	1.0250000000000001	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	25	0.625	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	21	0.525	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	21	0.525	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	20	0.5	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	18	0.44999999999999996	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	18	0.44999999999999996	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	17	0.42500000000000004	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	17	0.42500000000000004	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	17	0.42500000000000004	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	17	0.42500000000000004	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	15	0.375	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	15	0.375	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	15	0.375	No Hit
CATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCAC	14	0.35000000000000003	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	14	0.35000000000000003	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	14	0.35000000000000003	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	14	0.35000000000000003	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	14	0.35000000000000003	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	14	0.35000000000000003	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	13	0.325	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	13	0.325	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	12	0.3	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	12	0.3	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	12	0.3	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	12	0.3	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	12	0.3	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	11	0.27499999999999997	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	11	0.27499999999999997	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	11	0.27499999999999997	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	11	0.27499999999999997	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	11	0.27499999999999997	No Hit
GAGGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGG	11	0.27499999999999997	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	11	0.27499999999999997	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	10	0.25	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	10	0.25	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	10	0.25	No Hit
GAATTAGAACTCTAAACTTAAGCTAATAGAGTAGCTTATCGAAATATTAC	10	0.25	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	9	0.22499999999999998	No Hit
GAATCAGAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTC	9	0.22499999999999998	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	9	0.22499999999999998	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	9	0.22499999999999998	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	9	0.22499999999999998	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	9	0.22499999999999998	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	9	0.22499999999999998	No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC	8	0.2	No Hit
GTACATGGGGACATTACAACATCACCCCACATCTACAGACACCAAAGCCT	8	0.2	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	8	0.2	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	8	0.2	No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT	8	0.2	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	8	0.2	No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG	8	0.2	No Hit
GAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCG	8	0.2	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	8	0.2	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	8	0.2	No Hit
GGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAA	7	0.17500000000000002	No Hit
GGGCAGTGACAAGGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCT	7	0.17500000000000002	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	7	0.17500000000000002	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	7	0.17500000000000002	No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	7	0.17500000000000002	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	7	0.17500000000000002	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	7	0.17500000000000002	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	7	0.17500000000000002	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	7	0.17500000000000002	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	7	0.17500000000000002	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	7	0.17500000000000002	No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT	7	0.17500000000000002	No Hit
ACCAGACTCTGATTCCATGAGGAGGGCCTCAAGGACTTGAAGAAATCCTG	7	0.17500000000000002	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	7	0.17500000000000002	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	7	0.17500000000000002	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC	6	0.15	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	6	0.15	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	6	0.15	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	6	0.15	No Hit
GACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCTGCTTGTCTGACAAAG	6	0.15	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	6	0.15	No Hit
GGTGAACACAGTCGCTGGAGAGAAACTCCCCGAATGTGAGGCAGTGTGTG	6	0.15	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
GAGCAGCTTCCTGCTTGTCTGACAAAGACCCTTTGCGGAGGGTCTTTCCT	6	0.15	No Hit
GTTCTAATTCTTTTTACTGCTCAATCTTTTTAATTAAAAACTTATCTGCG	6	0.15	No Hit
GATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTCTCTTGTGGGAGGG	6	0.15	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	6	0.15	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	6	0.15	No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	6	0.15	No Hit
GAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTC	6	0.15	No Hit
GACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATT	5	0.125	No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT	5	0.125	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	5	0.125	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	5	0.125	No Hit
GGATTTTCTCCGTGAGCTTCATGATGTTTATCCTGGTCTCTTGTGGGAGG	5	0.125	No Hit
CCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGC	5	0.125	No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT	5	0.125	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	5	0.125	No Hit
GATCAGTACCCAACACCTGCACCCTAGTCATGTGTAGCCCAACAGAGAAT	5	0.125	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	5	0.125	No Hit
GCTTTGTGCAGTCCCAGCACCCAGTTAGAACAGCCTCTGTGGTGGGCCGG	5	0.125	No Hit
AGATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCT	5	0.125	No Hit
CTATTAGCTTAAGTTTAGAGTTCTAATTCTTTTTACTGCTCAATCTTTTT	5	0.125	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	5	0.125	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	5	0.125	No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT	5	0.125	No Hit
GGATAAACATCATGAAGCTCACGGAGAAAATCCTAACAAGTCCTCTGTGT	5	0.125	No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	5	0.125	No Hit
GGACAGAAGTTTGTGCCGGAAGCCCTGGGATCCCTGGCACTCCCGGAAAC	5	0.125	No Hit
GTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAGCATCCACA	5	0.125	No Hit
GGATTACTCGGCAGGTCCCAGGAGCCAGTTCCGCATCCCCTGCTGTCCCG	5	0.125	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	5	0.125	No Hit
CCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGG	5	0.125	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.025	0.0
28-29	0.0	0.0	0.0	0.025	0.0
30-31	0.0	0.0	0.0	0.075	0.0
32-33	0.0	0.0	0.0	0.1	0.0
34-35	0.0	0.0	0.0	0.125	0.0
36-37	0.0	0.0	0.0	0.1375	0.0
38-39	0.0	0.0	0.0	0.2	0.0
40-41	0.0	0.0	0.0	0.2375	0.0
42-43	0.0	0.0	0.0	0.35	0.0
44-45	0.0	0.0	0.0	0.425	0.0
46-47	0.0	0.0	0.0	0.575	0.0
48-49	0.0	0.0	0.0	0.7125	0.0
50-51	0.0	0.0	0.0	0.9125	0.0
52-53	0.0	0.0	0.0	1.05	0.0
54-55	0.0	0.0	0.0	1.1	0.0
56-57	0.0	0.0	0.0	1.25	0.0
58-59	0.0	0.0	0.0	1.475	0.0
60-61	0.0	0.0	0.0	1.6625	0.0
62-63	0.0	0.0	0.0	1.825	0.0
64-65	0.0	0.0	0.0	2.05	0.0
66-67	0.0	0.0	0.0	2.1875	0.0
68-69	0.0	0.0	0.0	2.4875	0.0
70-71	0.0	0.0	0.0	2.7625	0.0
72-73	0.0	0.0	0.0	3.0374999999999996	0.0
74-75	0.0	0.0	0.0	3.2125000000000004	0.0
76-77	0.0	0.0	0.0	3.425	0.0
78-79	0.0	0.0	0.0	3.675	0.0
80-81	0.0	0.0	0.0	3.9375	0.0
82-83	0.0	0.0	0.0	4.4375	0.0
84-85	0.0	0.0	0.0	4.699999999999999	0.0
86-87	0.0	0.0	0.0	4.975	0.0
88-89	0.0	0.0	0.0	5.175	0.0
90-91	0.0	0.0	0.0	5.387499999999999	0.0
92-93	0.0	0.0	0.0	5.6125	0.0
94-95	0.0	0.0	0.0	5.9875	0.0
96-97	0.0	0.0	0.0	6.4375	0.0
98-99	0.0	0.0	0.0	6.925000000000001	0.0
100-101	0.0	0.0	0.0	7.375	0.0
102-103	0.0	0.0	0.0	7.8375	0.0
104-105	0.0	0.0	0.0	8.1875	0.0
106-107	0.0	0.0	0.0	8.475000000000001	0.0
108-109	0.0	0.0	0.0	8.9375	0.0
110-111	0.0	0.0	0.0	9.5	0.0
112-113	0.0	0.0	0.0	10.162500000000001	0.0
114-115	0.0	0.0	0.0	10.712499999999999	0.0
116-117	0.0	0.0	0.0	11.075	0.0
118-119	0.0	0.0	0.0	11.4125	0.0
120-121	0.0	0.0	0.0	11.95	0.0
122-123	0.0	0.0	0.0	12.45	0.0
124-125	0.0	0.0	0.0	12.925	0.0
126-127	0.0	0.0	0.0	13.6	0.0
128-129	0.0	0.0	0.0	14.3	0.0
130-131	0.0	0.0	0.0	14.9875	0.0
132-133	0.0	0.0	0.0	15.875	0.0
134-135	0.0	0.0	0.0	16.5375	0.0
136-137	0.0	0.0	0.0	17.362499999999997	0.0
138	0.0	0.0	0.0	17.85	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTCA	10	0.006973645	144.0	1
ATGGTCC	10	0.006973645	144.0	4
GATGGTC	10	0.006973645	144.0	3
TTTTTTT	350	9.562162E-5	6.1714287	20-24
>>END_MODULE
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
Read 2785497 spots for SRR31990347.sra
Written 2785497 spots for SRR31990347.sra
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
Read 2785493 spots for SRR31990347.sra
Written 2785493 spots for SRR31990347.sra
SRR ids: ['SRR31990347.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_u5lvo9yn
SRR31990347.sra spots: 22283948
blocks: [[1, 2785493], [2785494, 5570986], [5570987, 8356479], [8356480, 11141972], [11141973, 13927465], [13927466, 16712958], [16712959, 19498451], [19498452, 22283948]]
SRR31990347 file size 8159976
SRR31990347 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990347 SRR31990347_1.fastq SRR31990347_2.fastq
Input file:	SRR31990347_1.fastq
Paired file:	SRR31990347_2.fastq
trimmed:	SRR31990347-trimmed-pair1.fastq, SRR31990347-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:54:20 2026 >> started

Sat Apr 25 17:54:51 2026 >> done (31.423s)
22283948 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
22283948 (100.00%) read pairs available; of these:
    4372 ( 0.02%) trimmed read pairs available after processing
22279576 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       2	  0.00%
 19	       0	  0.00%
 20	       0	  0.00%
 21	       2	  0.00%
 22	       2	  0.00%
 23	       3	  0.00%
 24	       5	  0.00%
 25	       9	  0.00%
 26	     713	  0.00%
 27	       9	  0.00%
 28	       6	  0.00%
 29	       3	  0.00%
 30	       2	  0.00%
 31	       2	  0.00%
 32	       1	  0.00%
 33	       4	  0.00%
 34	       2	  0.00%
 35	       2	  0.00%
 36	       2	  0.00%
 37	       2	  0.00%
 38	       3	  0.00%
 39	       3	  0.00%
 40	       4	  0.00%
 41	       0	  0.00%
 42	       4	  0.00%
 43	       3	  0.00%
 44	       4	  0.00%
 45	       6	  0.00%
 46	       4	  0.00%
 47	       5	  0.00%
 48	       1	  0.00%
 49	       0	  0.00%
 50	       1	  0.00%
 51	       1	  0.00%
 52	       7	  0.00%
 53	       2	  0.00%
 54	       1	  0.00%
 55	       2	  0.00%
 56	       2	  0.00%
 57	       4	  0.00%
 58	       3	  0.00%
 59	       1	  0.00%
 60	       6	  0.00%
 61	       3	  0.00%
 62	       2	  0.00%
 63	       3	  0.00%
 64	       2	  0.00%
 65	       3	  0.00%
 66	       2	  0.00%
 67	       1	  0.00%
 68	      14	  0.00%
 69	      99	  0.00%
 70	    1479	  0.01%
 71	      23	  0.00%
 72	       5	  0.00%
 73	       1	  0.00%
 74	       2	  0.00%
 75	       1	  0.00%
 76	       1	  0.00%
 77	       5	  0.00%
 78	       2	  0.00%
 79	       3	  0.00%
 80	       2	  0.00%
 81	       2	  0.00%
 82	       1	  0.00%
 83	       2	  0.00%
 84	       3	  0.00%
 85	       3	  0.00%
 86	       1	  0.00%
 87	       0	  0.00%
 88	       3	  0.00%
 89	       3	  0.00%
 90	       4	  0.00%
 91	       0	  0.00%
 92	       3	  0.00%
 93	       2	  0.00%
 94	       4	  0.00%
 95	       4	  0.00%
 96	       4	  0.00%
 97	       2	  0.00%
 98	       4	  0.00%
 99	       2	  0.00%
100	       1	  0.00%
101	       1	  0.00%
102	       3	  0.00%
103	       1	  0.00%
104	       5	  0.00%
105	       4	  0.00%
106	       2	  0.00%
107	       3	  0.00%
108	       1	  0.00%
109	       3	  0.00%
110	       5	  0.00%
111	       4	  0.00%
112	       2	  0.00%
113	       4	  0.00%
114	       5	  0.00%
115	       1	  0.00%
116	       5	  0.00%
117	       0	  0.00%
118	       5	  0.00%
119	       1	  0.00%
120	       3	  0.00%
121	       6	  0.00%
122	       1	  0.00%
123	       3	  0.00%
124	       2	  0.00%
125	       9	  0.00%
126	       4	  0.00%
127	       3	  0.00%
128	       2	  0.00%
129	       6	  0.00%
130	       3	  0.00%
131	       3	  0.00%
132	       2	  0.00%
133	       5	  0.00%
134	       3	  0.00%
135	       2	  0.00%
136	       6	  0.00%
137	       9	  0.00%
138	       2	  0.00%
139	       6	  0.00%
140	       5	  0.00%
141	       1	  0.00%
142	       2	  0.00%
143	       5	  0.00%
144	       3	  0.00%
145	       3	  0.00%
146	       5	  0.00%
147	      44	  0.00%
148	      55	  0.00%
149	    1575	  0.01%
150	22279576	 99.98%
22283948 reads passed initial QC


criterion=sequence-density
sequence-density=16.99
sequence-density-rank=1
fanout-score=34.53
fanout-score-rank=2
prefix-density=18.20
prefix-fanout=32.2
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=14
fanout-score=274.19
fanout-score-rank=1
prefix-density=3.32
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=17.21
sequence-density-rank=1
fanout-score=34.15
fanout-score-rank=2
prefix-density=18.44
prefix-fanout=31.9
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=14
fanout-score=368.24
fanout-score-rank=1
prefix-density=3.31
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAA -y CTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA -o SRR31990347 SRR31990347_1.fastq SRR31990347_2.fastq
Input file:	SRR31990347_1.fastq
Paired file:	SRR31990347_2.fastq
trimmed:	SRR31990347-trimmed-pair1.fastq, SRR31990347-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:55:48 2026 >> started

Sat Apr 25 17:56:27 2026 >> done (38.759s)
19807954 read pairs processed; of these:
      68 ( 0.00%) short read pairs filtered out after trimming by size control
       9 ( 0.00%) empty read pairs filtered out after trimming by size control
19807877 (100.00%) read pairs available; of these:
 4509643 (22.77%) trimmed read pairs available after processing
15298234 (77.23%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      10	  0.00%
 19	      22	  0.00%
 20	       8	  0.00%
 21	      40	  0.00%
 22	      20	  0.00%
 23	      54	  0.00%
 24	      43	  0.00%
 25	     111	  0.00%
 26	     726	  0.00%
 27	     299	  0.00%
 28	     671	  0.00%
 29	     880	  0.00%
 30	    1148	  0.01%
 31	    1979	  0.01%
 32	    1810	  0.01%
 33	    2991	  0.02%
 34	    2572	  0.01%
 35	    3975	  0.02%
 36	    6972	  0.04%
 37	    9015	  0.05%
 38	   11232	  0.06%
 39	   10745	  0.05%
 40	   12859	  0.06%
 41	   11310	  0.06%
 42	   11853	  0.06%
 43	   11477	  0.06%
 44	   17366	  0.09%
 45	   14517	  0.07%
 46	   19648	  0.10%
 47	   17406	  0.09%
 48	   20877	  0.11%
 49	   16658	  0.08%
 50	   18139	  0.09%
 51	   15137	  0.08%
 52	   15678	  0.08%
 53	   16431	  0.08%
 54	   16226	  0.08%
 55	   27250	  0.14%
 56	   19705	  0.10%
 57	   18428	  0.09%
 58	   17713	  0.09%
 59	   16574	  0.08%
 60	   19237	  0.10%
 61	   17996	  0.09%
 62	   21271	  0.11%
 63	   24297	  0.12%
 64	   18242	  0.09%
 65	   17414	  0.09%
 66	   25804	  0.13%
 67	   24458	  0.12%
 68	   21194	  0.11%
 69	   30916	  0.16%
 70	   25160	  0.13%
 71	   27753	  0.14%
 72	   24694	  0.12%
 73	   23409	  0.12%
 74	   24954	  0.13%
 75	   27790	  0.14%
 76	   24860	  0.13%
 77	   20999	  0.11%
 78	   22326	  0.11%
 79	   21317	  0.11%
 80	   31277	  0.16%
 81	   45774	  0.23%
 82	   37209	  0.19%
 83	   22410	  0.11%
 84	   23767	  0.12%
 85	   27199	  0.14%
 86	   30969	  0.16%
 87	   34657	  0.17%
 88	   31228	  0.16%
 89	   36801	  0.19%
 90	   31883	  0.16%
 91	   29091	  0.15%
 92	   31250	  0.16%
 93	   37438	  0.19%
 94	   33618	  0.17%
 95	   41988	  0.21%
 96	   37758	  0.19%
 97	   31058	  0.16%
 98	   44510	  0.22%
 99	   44010	  0.22%
100	   38661	  0.20%
101	   39446	  0.20%
102	   36617	  0.18%
103	   44683	  0.23%
104	   46020	  0.23%
105	   51406	  0.26%
106	   46478	  0.23%
107	   48859	  0.25%
108	   49999	  0.25%
109	   48496	  0.24%
110	   43645	  0.22%
111	   48863	  0.25%
112	   48233	  0.24%
113	   49524	  0.25%
114	   49825	  0.25%
115	   40846	  0.21%
116	   41502	  0.21%
117	   44260	  0.22%
118	   41042	  0.21%
119	   53606	  0.27%
120	   52246	  0.26%
121	   56016	  0.28%
122	   56280	  0.28%
123	   61438	  0.31%
124	   53923	  0.27%
125	   57491	  0.29%
126	   60009	  0.30%
127	   59901	  0.30%
128	   49011	  0.25%
129	   62380	  0.31%
130	   74278	  0.37%
131	   62886	  0.32%
132	   66094	  0.33%
133	   75350	  0.38%
134	   64075	  0.32%
135	   84852	  0.43%
136	   71297	  0.36%
137	   57380	  0.29%
138	   67488	  0.34%
139	   70348	  0.36%
140	   81677	  0.41%
141	   75416	  0.38%
142	   81845	  0.41%
143	   71982	  0.36%
144	   78395	  0.40%
145	   61917	  0.31%
146	   77670	  0.39%
147	   84393	  0.43%
148	   90795	  0.46%
149	   96140	  0.49%
150	15294332	 77.21%


criterion=sequence-density
sequence-density=2.00
sequence-density-rank=1
fanout-score=2.05
fanout-score-rank=30
prefix-density=2.05
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=287.07
fanout-score-rank=1
prefix-density=3.32
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=2.05
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=32
prefix-density=2.06
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=372.58
fanout-score-rank=1
prefix-density=3.29
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990347 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 17:57:46
                             Started mapping on |	Apr 25 17:57:46
                                    Finished on |	Apr 25 18:00:06
       Mapping speed, Million of reads per hour |	573.01

                          Number of input reads |	22283871
                      Average input read length |	275
                                    UNIQUE READS:
                   Uniquely mapped reads number |	16006875
                        Uniquely mapped reads % |	71.83%
                          Average mapped length |	281.14
                       Number of splices: Total |	15150653
            Number of splices: Annotated (sjdb) |	15053685
                       Number of splices: GT/AG |	15080033
                       Number of splices: GC/AG |	23489
                       Number of splices: AT/AC |	3619
               Number of splices: Non-canonical |	43512
                      Mismatch rate per base, % |	0.28%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.46
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1886566
             % of reads mapped to multiple loci |	8.47%
        Number of reads mapped to too many loci |	44040
             % of reads mapped to too many loci |	0.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	19.35%
                     % of reads unmapped: other |	0.15%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4390430	4390430	4390430
N_multimapping	1886566	1886566	1886566
N_noFeature	1585550	8316734	8286231
N_ambiguous	1368806	204215	203956
UnstrandedReadsAssigned:13052519 PositiveStrandReadsAssigned:7485926 NegativeStrandReadsAssigned:7516688
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=143 echo kmer=139
SRR31990347 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990347-trimmed-pair1.fastq
                             SRR31990347-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,283,871 reads, 17,985,326 reads pseudoaligned
[quant] estimated average fragment length: 201.284
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,124 rounds

 109283 SRR31990347.ke.tsv
  52636 SRR31990347.se.tsv
 161919 total
==> SRR31990347.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	15.3333	0	0
ENSMUST00000196221.1	9	8.33333	0	0
ENSMUST00000179664.1	11	10.3333	0	0
ENSMUST00000178537.1	12	11.3333	0	0
ENSMUST00000178862.1	14	13.3333	0	0
ENSMUST00000179520.1	11	10.3333	0	0
ENSMUST00000179883.1	16	15.3333	0	0
ENSMUST00000195858.1	10	9.33333	0	0
ENSMUST00000179932.1	12	11.3333	0	0

==> SRR31990347.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	1
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	1
ENSMUSG00000103147	0
SRR31990347 completed mapping pipeline successfully
