Starting /dee2/code/volunteer_pipeline.sh SRR31990354
    current disk space = 33518052
    free memory = 191636080 
SRR31990354 SRAfilesize
7856d94b8bef3f7b3c2a8805a6ca4459  SRR31990354.sra
SRR31990354.sra file validated
SRR31990354 is paired end
SRR31990354 is conventional basespace
SRR31990354 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9345	37.0	37.0	37.0	25.0	37.0
2	30.96275	37.0	25.0	37.0	11.0	37.0
3	32.73	37.0	37.0	37.0	25.0	37.0
4	33.001	37.0	37.0	37.0	25.0	37.0
5	34.1565	37.0	37.0	37.0	25.0	37.0
6	34.38	37.0	37.0	37.0	25.0	37.0
7	34.467	37.0	37.0	37.0	25.0	37.0
8	34.8975	37.0	37.0	37.0	25.0	37.0
9	34.964	37.0	37.0	37.0	25.0	37.0
10-14	34.868199999999995	37.0	37.0	37.0	25.0	37.0
15-19	34.879	37.0	37.0	37.0	25.0	37.0
20-24	34.9412	37.0	37.0	37.0	25.0	37.0
25-29	34.3994	37.0	37.0	37.0	25.0	37.0
30-34	34.750800000000005	37.0	37.0	37.0	25.0	37.0
35-39	34.9255	37.0	37.0	37.0	25.0	37.0
40-44	34.59060000000001	37.0	37.0	37.0	25.0	37.0
45-49	34.614799999999995	37.0	37.0	37.0	25.0	37.0
50-54	34.254599999999996	37.0	37.0	37.0	25.0	37.0
55-59	34.3346	37.0	37.0	37.0	25.0	37.0
60-64	34.3565	37.0	37.0	37.0	25.0	37.0
65-69	34.1585	37.0	37.0	37.0	25.0	37.0
70-74	34.0139	37.0	37.0	37.0	22.2	37.0
75-79	34.1753	37.0	37.0	37.0	25.0	37.0
80-84	33.9919	37.0	37.0	37.0	25.0	37.0
85-89	34.067099999999996	37.0	37.0	37.0	25.0	37.0
90-94	33.89149999999999	37.0	37.0	37.0	25.0	37.0
95-99	33.6945	37.0	37.0	37.0	25.0	37.0
100-104	33.720299999999995	37.0	37.0	37.0	25.0	37.0
105-109	33.437	37.0	37.0	37.0	22.2	37.0
110-114	33.2889	37.0	37.0	37.0	22.2	37.0
115-119	33.2459	37.0	37.0	37.0	22.2	37.0
120-124	32.958600000000004	37.0	34.6	37.0	19.4	37.0
125-129	33.03009999999999	37.0	37.0	37.0	16.6	37.0
130-134	32.9932	37.0	37.0	37.0	16.6	37.0
135-139	32.8716	37.0	37.0	37.0	11.0	37.0
140-144	32.5968	37.0	34.6	37.0	11.0	37.0
145-149	31.891400000000004	37.0	29.8	37.0	11.0	37.0
150	32.2795	37.0	37.0	37.0	11.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	2.0
21	9.0
22	10.0
23	11.0
24	9.0
25	16.0
26	17.0
27	43.0
28	50.0
29	86.0
30	139.0
31	184.0
32	324.0
33	617.0
34	1050.0
35	1128.0
36	300.0
37	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.35	11.225	4.75	25.674999999999997
2	20.55485498108449	19.697351828499368	34.60277427490542	25.145018915510718
3	21.099999999999998	27.450000000000003	23.724999999999998	27.725
4	8.4	10.525	43.7	37.375
5	11.15	39.15	37.574999999999996	12.125
6	34.225	41.475	15.525	8.774999999999999
7	27.675	30.95	21.3	20.075000000000003
8	26.825	33.625	19.325	20.225
9	27.3	11.325000000000001	12.425	48.949999999999996
10-14	23.52	23.169999999999998	30.7	22.61
15-19	23.49	28.79	24.21	23.51
20-24	25.27	25.374999999999996	24.465	24.89
25-29	22.98	25.174999999999997	25.405	26.44
30-34	24.154999999999998	26.66	24.990000000000002	24.195
35-39	23.955000000000002	26.174999999999997	25.53	24.34
40-44	24.395	27.02	24.365000000000002	24.22
45-49	24.595	24.55	25.46	25.395
50-54	23.445	24.89	26.36	25.305
55-59	24.085	25.195	26.029999999999998	24.69
60-64	24.27	26.450000000000003	24.89	24.39
65-69	24.585	25.074999999999996	25.480000000000004	24.86
70-74	23.400000000000002	24.805	26.705000000000002	25.09
75-79	25.135	25.679999999999996	24.975	24.21
80-84	25.014999999999997	25.55	24.485	24.95
85-89	24.759999999999998	24.26	24.68	26.3
90-94	24.615000000000002	25.290000000000003	26.05	24.044999999999998
95-99	24.185000000000002	25.635	25.705	24.474999999999998
100-104	25.205	24.525	25.895000000000003	24.375
105-109	24.63	24.884999999999998	24.915000000000003	25.569999999999997
110-114	25.669999999999998	24.965	24.02	25.345000000000002
115-119	25.230000000000004	24.015	26.075	24.68
120-124	25.825	25.5	24.525	24.15
125-129	25.905	24.895	25.21	23.990000000000002
130-134	26.215	24.185000000000002	24.65	24.95
135-139	27.215	23.995	24.485	24.305
140-144	26.375	24.18	25.41	24.035
145-149	26.61	24.03	24.185000000000002	25.174999999999997
150	25.674999999999997	27.3	23.674999999999997	23.35
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	1.0
20	1.0
21	1.0
22	2.0
23	4.0
24	7.0
25	8.5
26	11.0
27	18.0
28	25.0
29	23.0
30	21.5
31	26.5
32	34.0
33	43.0
34	49.5
35	61.0
36	70.0
37	71.5
38	77.0
39	82.5
40	106.5
41	123.0
42	104.0
43	89.5
44	95.5
45	87.0
46	80.0
47	101.5
48	114.5
49	132.5
50	142.0
51	143.0
52	181.5
53	236.5
54	247.5
55	210.5
56	197.0
57	173.0
58	156.5
59	161.0
60	142.5
61	109.5
62	69.5
63	41.5
64	32.5
65	32.5
66	19.5
67	8.5
68	4.5
69	4.5
70	3.0
71	0.5
72	0.5
73	1.5
74	2.0
75	1.5
76	0.5
77	0.5
78	0.5
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.8750000000000001
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.65621697780908	59.375
2	9.686509334272632	13.750000000000002
3	2.3599859105318775	5.025
4	1.5146178231771752	4.3
5	0.7749207467418106	2.75
6	0.42268404367735113	1.7999999999999998
7	0.10567101091933778	0.525
8	0.28178936245156744	1.6
9	0.3522367030644593	2.25
>10	0.8453680873547023	8.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	33	0.8250000000000001	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	25	0.625	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	23	0.575	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	22	0.5499999999999999	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	21	0.525	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	19	0.475	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	17	0.42500000000000004	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	14	0.35000000000000003	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	12	0.3	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	12	0.3	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	11	0.27499999999999997	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	11	0.27499999999999997	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	11	0.27499999999999997	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	11	0.27499999999999997	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	11	0.27499999999999997	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	11	0.27499999999999997	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	11	0.27499999999999997	No Hit
GGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGC	10	0.25	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	10	0.25	No Hit
GTGTTGTACTTCTTTGTAATGCTTGCGATGGCCTCGTTCTCCTCAGGATT	10	0.25	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	10	0.25	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	10	0.25	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	10	0.25	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	10	0.25	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	9	0.22499999999999998	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	9	0.22499999999999998	No Hit
CTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGC	9	0.22499999999999998	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	9	0.22499999999999998	No Hit
CCCATGAGCAGAGCCCCTACAATCACCACGACAACGAGGACCACCACCAC	9	0.22499999999999998	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	9	0.22499999999999998	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	9	0.22499999999999998	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	9	0.22499999999999998	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	9	0.22499999999999998	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	9	0.22499999999999998	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	8	0.2	No Hit
GAGAGAAACCTTACAAAATGGACATGAGTAGCAAAGAGGTCCTGATGGAG	8	0.2	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	8	0.2	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	8	0.2	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	8	0.2	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	8	0.2	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	8	0.2	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	8	0.2	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	7	0.17500000000000002	No Hit
GCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGGCC	7	0.17500000000000002	No Hit
GTCCATCTGCTGCAGCTCAGCTTCTTCGGACATCTGCCCAGGATTTCTTC	7	0.17500000000000002	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	6	0.15	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	6	0.15	No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG	6	0.15	No Hit
GTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAGGAAAGCCAGG	6	0.15	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	6	0.15	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	6	0.15	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	6	0.15	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	6	0.15	No Hit
GGATTACTCGGCAGGTCCCAGGAGCCAGTTCCGCATCCCCTGCTGTCCCG	6	0.15	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	6	0.15	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	6	0.15	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	6	0.15	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC	5	0.125	No Hit
CCTTAACACTGTGCACTACGACTCGGACCAGTTCAAGACGCCTCAGGAGT	5	0.125	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	5	0.125	No Hit
GAGAGACACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAG	5	0.125	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	5	0.125	No Hit
GAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCAT	5	0.125	No Hit
AGATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCT	5	0.125	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	5	0.125	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	5	0.125	No Hit
GCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGAAATCGCAGAT	5	0.125	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	5	0.125	No Hit
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGT	5	0.125	No Hit
CTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTG	5	0.125	No Hit
GTTTTACCCCATTCATCTTGAGATGGCTTCTGCACATCCTGGAAGAGTGC	5	0.125	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	5	0.125	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	5	0.125	No Hit
CCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGCATCCCTGA	5	0.125	No Hit
CCCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAG	5	0.125	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	5	0.125	No Hit
GGGCAAAACAGAGACTTCCTCCTCACAAGCTTCAGCTGATGCTGTGTTGT	5	0.125	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	5	0.125	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0125	0.0
40-41	0.0	0.0	0.0	0.125	0.0
42-43	0.0	0.0	0.0	0.15	0.0
44-45	0.0	0.0	0.0	0.15	0.0
46-47	0.0	0.0	0.0	0.2	0.0
48-49	0.0	0.0	0.0	0.2625	0.0
50-51	0.0	0.0	0.0	0.325	0.0
52-53	0.0	0.0	0.0	0.425	0.0
54-55	0.0	0.0	0.0	0.5625	0.0
56-57	0.0	0.0	0.0	0.6875	0.0
58-59	0.0	0.0	0.0	0.775	0.0
60-61	0.0	0.0	0.0	0.825	0.0
62-63	0.0	0.0	0.0	0.9375	0.0
64-65	0.0	0.0	0.0	0.9875	0.0
66-67	0.0	0.0	0.0	1.0875	0.0
68-69	0.0	0.0	0.0	1.2375	0.0
70-71	0.0	0.0	0.0	1.3125	0.0
72-73	0.0	0.0	0.0	1.35	0.0
74-75	0.0	0.0	0.0	1.525	0.0
76-77	0.0	0.0	0.0	1.7	0.0
78-79	0.0	0.0	0.0	1.775	0.0
80-81	0.0	0.0	0.0	1.9125	0.0
82-83	0.0	0.0	0.0	2.075	0.0
84-85	0.0	0.0	0.0	2.1624999999999996	0.0
86-87	0.0	0.0	0.0	2.25	0.0
88-89	0.0	0.0	0.0	2.4625	0.0
90-91	0.0	0.0	0.0	2.7	0.0
92-93	0.0	0.0	0.0	2.9375	0.0
94-95	0.0	0.0	0.0	3.125	0.0
96-97	0.0	0.0	0.0	3.275	0.0
98-99	0.0	0.0	0.0	3.4625000000000004	0.0
100-101	0.0	0.0	0.0	3.8125	0.0
102-103	0.0	0.0	0.0	3.95	0.0
104-105	0.0	0.0	0.0	4.075	0.0
106-107	0.0	0.0	0.0	4.275	0.0
108-109	0.0	0.0	0.0	4.625	0.0
110-111	0.0	0.0	0.0	4.8125	0.0
112-113	0.0	0.0	0.0	5.1	0.0
114-115	0.0	0.0	0.0	5.324999999999999	0.0
116-117	0.0	0.0	0.0	5.375	0.0
118-119	0.0	0.0	0.0	5.675	0.0
120-121	0.0	0.0	0.0	6.0375	0.0
122-123	0.0	0.0	0.0	6.3875	0.0
124-125	0.0	0.0	0.0	6.775	0.0
126-127	0.0	0.0	0.0	7.0875	0.0
128-129	0.0	0.0	0.0	7.324999999999999	0.0
130-131	0.0	0.0	0.0	7.6625	0.0
132-133	0.0	0.0	0.0	8.1	0.0
134-135	0.0	0.0	0.0	8.55	0.0
136-137	0.0	0.0	0.0	8.8875	0.0
138	0.0	0.0	0.0	9.15	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTAT	10	0.006973645	144.0	9
CCCAGGT	10	0.006973645	144.0	7
GTACTGT	10	0.006973645	144.0	7
GTATTTG	10	0.006973645	144.0	1
TACTGTA	10	0.006973645	144.0	8
TATTTGT	15	1.1730364E-4	144.0	2
TGTAGCC	10	0.006973645	144.0	2
ATTTGTA	10	0.006973645	144.0	3
CAGGTCA	10	0.006973645	144.0	9
GCTCTGC	10	0.006973645	144.0	1
TTTGTAC	10	0.006973645	144.0	4
GGGGGGG	90	3.466812E-4	12.8	140-144
>>END_MODULE
SRR31990354 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR31990354_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.889	37.0	37.0	37.0	37.0	37.0
2	36.5015	37.0	37.0	37.0	37.0	37.0
3	36.297	37.0	37.0	37.0	37.0	37.0
4	36.567	37.0	37.0	37.0	37.0	37.0
5	36.402	37.0	37.0	37.0	37.0	37.0
6	36.5415	37.0	37.0	37.0	37.0	37.0
7	36.597	37.0	37.0	37.0	37.0	37.0
8	36.558	37.0	37.0	37.0	37.0	37.0
9	36.553	37.0	37.0	37.0	37.0	37.0
10-14	36.480599999999995	37.0	37.0	37.0	37.0	37.0
15-19	36.4142	37.0	37.0	37.0	37.0	37.0
20-24	36.3532	37.0	37.0	37.0	37.0	37.0
25-29	36.4055	37.0	37.0	37.0	37.0	37.0
30-34	36.3937	37.0	37.0	37.0	37.0	37.0
35-39	36.388000000000005	37.0	37.0	37.0	37.0	37.0
40-44	36.2208	37.0	37.0	37.0	37.0	37.0
45-49	36.1971	37.0	37.0	37.0	37.0	37.0
50-54	36.118399999999994	37.0	37.0	37.0	37.0	37.0
55-59	36.0532	37.0	37.0	37.0	37.0	37.0
60-64	36.09310000000001	37.0	37.0	37.0	37.0	37.0
65-69	36.0638	37.0	37.0	37.0	37.0	37.0
70-74	35.8333	37.0	37.0	37.0	37.0	37.0
75-79	35.9624	37.0	37.0	37.0	37.0	37.0
80-84	35.855399999999996	37.0	37.0	37.0	37.0	37.0
85-89	35.7425	37.0	37.0	37.0	37.0	37.0
90-94	35.665800000000004	37.0	37.0	37.0	37.0	37.0
95-99	35.6513	37.0	37.0	37.0	37.0	37.0
100-104	35.62949999999999	37.0	37.0	37.0	37.0	37.0
105-109	35.7509	37.0	37.0	37.0	37.0	37.0
110-114	35.5295	37.0	37.0	37.0	37.0	37.0
115-119	35.50690000000001	37.0	37.0	37.0	34.6	37.0
120-124	35.472300000000004	37.0	37.0	37.0	37.0	37.0
125-129	35.4193	37.0	37.0	37.0	34.6	37.0
130-134	35.1732	37.0	37.0	37.0	32.2	37.0
135-139	35.35850000000001	37.0	37.0	37.0	34.6	37.0
140-144	35.2459	37.0	37.0	37.0	29.8	37.0
145-149	35.054899999999996	37.0	37.0	37.0	27.4	37.0
150	35.0365	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	2.0
20	2.0
21	6.0
22	9.0
23	6.0
24	10.0
25	10.0
26	6.0
27	3.0
28	10.0
29	10.0
30	14.0
31	22.0
32	47.0
33	61.0
34	229.0
35	787.0
36	2640.0
37	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	60.5	9.975000000000001	4.425	25.1
2	20.8	18.425	37.275000000000006	23.5
3	20.525	27.900000000000002	24.9	26.674999999999997
4	7.6	11.275	43.65	37.475
5	10.325	40.8	36.75	12.125
6	34.300000000000004	42.5	15.174999999999999	8.025
7	26.6	31.525	22.275	19.6
8	28.249999999999996	31.65	19.0	21.099999999999998
9	27.3	11.025	13.525	48.15
10-14	23.625	22.585	31.455	22.335
15-19	23.87	27.785	24.93	23.415
20-24	25.105	24.72	25.745	24.43
25-29	23.435	25.305	25.564999999999998	25.695
30-34	24.59	26.1	25.135	24.175
35-39	23.955000000000002	25.86	26.245	23.94
40-44	24.37	26.945000000000004	24.895	23.79
45-49	24.09	24.93	25.635	25.345000000000002
50-54	23.919999999999998	25.91	25.885	24.285
55-59	24.2	25.05	25.735000000000003	25.014999999999997
60-64	24.86	25.480000000000004	25.650000000000002	24.01
65-69	24.46	25.44	25.724999999999998	24.375
70-74	24.240000000000002	24.959999999999997	26.290000000000003	24.51
75-79	25.185000000000002	25.64	25.185000000000002	23.990000000000002
80-84	25.585	24.87	24.515	25.03
85-89	24.455	24.21	25.224999999999998	26.11
90-94	24.54	25.345000000000002	26.465	23.65
95-99	23.724999999999998	25.1	26.61	24.565
100-104	24.884999999999998	24.625	25.97	24.52
105-109	24.25	24.88	25.195	25.674999999999997
110-114	25.264999999999997	24.685000000000002	25.374999999999996	24.675
115-119	25.05	24.07	26.650000000000002	24.23
120-124	24.09	26.11	25.595000000000002	24.205
125-129	25.235000000000003	25.115	26.009999999999998	23.64
130-134	25.330000000000002	24.04	25.85	24.779999999999998
135-139	24.705	24.765	25.365	25.165
140-144	24.65	24.36	25.935000000000002	25.055
145-149	25.255	24.395	25.235000000000003	25.115
150	25.874999999999996	26.025	24.725	23.375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.5
15	0.5
16	0.0
17	0.0
18	1.5
19	3.0
20	3.0
21	2.0
22	1.0
23	2.0
24	3.0
25	4.0
26	12.5
27	23.0
28	26.5
29	25.0
30	24.5
31	20.5
32	19.5
33	42.0
34	53.0
35	62.0
36	78.5
37	74.5
38	79.0
39	90.0
40	107.5
41	123.5
42	102.0
43	82.5
44	97.5
45	99.5
46	102.5
47	107.5
48	102.5
49	135.5
50	147.5
51	141.5
52	193.5
53	222.5
54	234.0
55	225.0
56	193.5
57	167.5
58	153.0
59	164.0
60	142.0
61	99.0
62	59.5
63	44.0
64	36.5
65	24.5
66	15.5
67	8.0
68	6.0
69	3.5
70	1.5
71	1.0
72	1.5
73	1.0
74	0.0
75	0.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.92289810151104	50.925
2	12.320805889190236	15.9
3	3.2932971716388995	6.375
4	1.6272762495156916	4.2
5	0.8911274699728787	2.875
6	0.65865943432778	2.55
7	0.5424254165052305	2.45
8	0.38744672607516467	2.0
9	0.1162340178225494	0.675
>10	1.239829523440527	12.049999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCTTTAGCAGTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAA	43	1.075	No Hit
CCATTATACAGTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTT	26	0.65	No Hit
GCTTTATTCTTTTGTGATAGGATCCCCCTGGCCCGTAGGAGAGACACCTT	23	0.575	No Hit
GCCTAGCCCCGAGTGAGCGAGCAGACACCATCGCTACCTTTTCCATCGGC	20	0.5	No Hit
CAATCACTGTGGTCATGCTGTCCATCTGCTGCAGCTCAGCTTCTTCGGAC	20	0.5	No Hit
GTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTT	18	0.44999999999999996	No Hit
GCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCCGAGGA	18	0.44999999999999996	No Hit
CAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCGGACTCGGAA	18	0.44999999999999996	No Hit
CTGCTGTCCCGTGCACCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGG	16	0.4	No Hit
GTATCATGCCCTTCCTCCTGGCCCAGCTTAGAGGTGGGTGTGGAGGGCTT	16	0.4	No Hit
ATATAGTAGAGTGGTAGCTCTCCACACAGGGTGCTCACAGCAAGGCCTAG	15	0.375	No Hit
GTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGGCCAAGCCCTCC	15	0.375	No Hit
GTCAAAAACATACTGAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAA	15	0.375	No Hit
GATTAAAGGCGTGCGCCACCATCGCCCGGCTAGCCTAACACTTCCATCTT	15	0.375	No Hit
GCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTCC	14	0.35000000000000003	No Hit
TGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTTCATGATGTT	14	0.35000000000000003	No Hit
GCTTATAGGAGACCAGTTGATCTTGGGAAACAGCAGTCGTGTGAGCTGCA	13	0.325	No Hit
GGGCTAGGCGTTTCTGAGTTTCCGGTGCTCCGATGCTCATCTCAAGGACC	13	0.325	No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA	13	0.325	No Hit
CCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCT	12	0.3	No Hit
CAGTAAAGTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGAT	12	0.3	No Hit
GACAAAGACCCTTTGCGGAGGGTCTTTCCTGTCCCGCTGCGGTTTCTACC	11	0.27499999999999997	No Hit
GTAGAATATCTCTGAAATCCAGTGAGCTTCAGAATCTTAAATCTTGCTTA	11	0.27499999999999997	No Hit
GCCTTCATGTTATCTCTTAAGATCTTCTTAGATTATTAAGACTAAGTAAT	11	0.27499999999999997	No Hit
GTACATGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGCC	10	0.25	No Hit
GCCTTGCTGTGAGCACCCTGTGTGGAGAGCTACCACTCTACTATATCTAG	10	0.25	No Hit
GAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAACTTCCAGG	10	0.25	No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT	10	0.25	No Hit
CCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAGATCGCC	10	0.25	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	10	0.25	No Hit
GGCATGATACTGGTTCCGAGTCCGATTCTTCCGGGAGAGACCTGGCTTTC	10	0.25	No Hit
GTATCCACCAGTCTCTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCC	10	0.25	No Hit
CCTCTAAGCTGGGCCAGGAGGAAGGGCATGATACTGGTTCCGAGTCCGAT	9	0.22499999999999998	No Hit
GCTCATGGGCCTCCACATGAGTCAAAAACATACTGAGATGGTCCTTGAGA	9	0.22499999999999998	No Hit
GGCCAATGCTGCAGGCCACCTCGATGACCTGCCCGGTGCCCTGTCTGCTC	9	0.22499999999999998	No Hit
CCTCAAGACTAGGGATGCTCTCTGGAGCCATCTTCATGATGTAGCAGTAG	8	0.2	No Hit
GAGCAGCGTAGAGCAGCACAGCTGAGCTCGTGAGGCAGGAGACTCAGCCC	8	0.2	No Hit
GGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAGGAGGGCCTC	8	0.2	No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC	8	0.2	No Hit
GTACAAATACTTTTCTCCTCATTTCCTTTTTCAAACTGTTTAAATAAGCC	8	0.2	No Hit
GTCCAAGACTTGACAGAGGCTCCAGGGCGGTTACAAAGCTGCCTAGTGGC	8	0.2	No Hit
GGGTTAAACAGACTGACACATGAGCTGAGAATTTATTTTTTAAGCAAGAT	8	0.2	No Hit
GCTCCAGAGAGCATCCCTAGTCTTGAGGCTTTCGCTAGAAAACTCCAGAA	8	0.2	No Hit
CCTCAAACGCCTTCTCATCGTGGTTGTGGTGGTGGTCCTCGTTGTCGTGG	8	0.2	No Hit
GTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCG	8	0.2	No Hit
CCTGAAGCCTTTCAACCCTGGCTCAGACCTGCAAAATGCGGGCACCCAGC	7	0.17500000000000002	No Hit
GTATTTGTACTGTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTA	7	0.17500000000000002	No Hit
CTCTGGAGCCATCTTCATGATGTAGCAGTAGGTTCCTGGAGCTGGCTTAT	7	0.17500000000000002	No Hit
GGCTTATAGGCCGTCAGGAGCCGCTGGTAGTCATACACAACGATGCCAGT	7	0.17500000000000002	No Hit
CTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCTGGTTATGTGGC	7	0.17500000000000002	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	7	0.17500000000000002	No Hit
GTTTTACCCCATTCATCTTGAGATGGCTTCTGCACATCCTGGAAGAGTGC	7	0.17500000000000002	No Hit
GGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAG	7	0.17500000000000002	No Hit
GCCCAGGATTTCTTCAAGTCCTTGAGGCCCTCCTCATGGAATCAGAGTCT	7	0.17500000000000002	No Hit
CTACTATATCTAGCATCCACAGGGTCGGTAGAAACCGCAGCGGGACAGGA	7	0.17500000000000002	No Hit
GAGATGGTCCTTGAGATGAGCATCGGAGCACCGGAAACTCAGAAACGCCT	7	0.17500000000000002	No Hit
CTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAG	7	0.17500000000000002	No Hit
GGGTGCTCACAGCAAGGCCTAGGAAAGCCAGGTCTCTCCCGGAAGAATCG	7	0.17500000000000002	No Hit
CTTCAGCTGGGTGCCCGCATTTTGCAGGTCTGAGCCAGGGTTGAAAGGCT	7	0.17500000000000002	No Hit
GTTCTAGTTTGAAGGTCGGCCTTGTAGATTAAAACGAAGGTTACCTAAAT	6	0.15	No Hit
TGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTGG	6	0.15	No Hit
GTCCACCGGATTACTCGGCAGGTCCCAGGAGCCAGTTCCGCATCCCCTGC	6	0.15	No Hit
CCCCTGGCCCGTAGGAGAGACACCTTTCCTTTACAGACTTCCACCGGTTT	6	0.15	No Hit
GGTCAGGGGGGATGCCCTCTTTATCCTGGATCTTGGCCTTCACGTTCTCG	6	0.15	No Hit
GGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCA	6	0.15	No Hit
GAGTCTGGTTATGTGGCATCCCTGAAGCCTTTCAACCCTGGCTCAGACCT	6	0.15	No Hit
GTTCTGGAGTTTTCTAGCGAAAGCCTCAAGACTAGGGATGCTCTCTGGAG	6	0.15	No Hit
GATGTGTACCAGAGCCGGTGGCCACATTGCTGCCCCGAGGAATCCTGAGG	6	0.15	No Hit
GTCTTTCCTGTCCCGCTGCGGTTTCTACCGACCCTGTGGATGCTAGATAT	6	0.15	No Hit
AGCCTAACCTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGG	6	0.15	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	6	0.15	No Hit
CACCTTTCCTTTACAGACTTCCACCGGTTTCTGGGCAGGAGCAGCTTCCT	6	0.15	No Hit
GGACAGAAGTTTGTGCCGGAAGCCCTGGGATCCCTGGCACTCCCGGAAAC	6	0.15	No Hit
GGACAGGCACAGCTCACTAGTCGCTCCTGAATGCCTCGTGACACTGGGAA	6	0.15	No Hit
GTTGTAGAGAATAGTTTGAAAATGAGGTGTAGTTTTAAAAGATTGAGAAA	6	0.15	No Hit
GTGCAGAGTAATGCCATCACTGCCCACTGGGAATGCAGACCGAATAATTA	6	0.15	No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT	5	0.125	No Hit
GGGTTGAAAGGCTTCAGGGATGCCACATAACCAGACTCTGATTCCATGAG	5	0.125	No Hit
GGTGGATACCCTCCCACAAGAGACCAGGATAAACATCATGAAGCTCACGG	5	0.125	No Hit
CTTAAGATCTTCTTAGATTATTAAGACTAAGTAATATTTCGATAAGCTAC	5	0.125	No Hit
GGACAGCAGGGGATGCGGAACTGGCTCCTGGGACCTGCCGAGTAATCCGG	5	0.125	No Hit
GTACATGGGGACACATTACAACATCACCCCACATCTACAGACACCAAAGC	5	0.125	No Hit
GCTTTGTGCAGTCCCAGCACCCAGTTAGAACAGCCTCTGTGGTGGGCCGG	5	0.125	No Hit
CACCAAAGCCTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTG	5	0.125	No Hit
GGAAGGAGGGGTTGGTGTGGGGAAGCCGAGGAGCCGAGGAGACACAGGGC	5	0.125	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	5	0.125	No Hit
CTCCAGAACTTCCAGGCCAAGCCCTCCACACCCACCTCTAAGCTGGGCCA	5	0.125	No Hit
GTACATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	5	0.125	No Hit
CTCCAACCTCTACCATGAAGATCGCCATCACAATCACTGTGGTCATGCTG	5	0.125	No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT	5	0.125	No Hit
ACATCACCCCACATCTACAGACACCAAAGCCTCCAACCTCTACCATGAAG	5	0.125	No Hit
GTACATGGGGGAGGAGAGGAGAGAGAGAGAGAAACCTTACAAAATGGACA	5	0.125	No Hit
ATGTTTATCCTGGTCTCTTGTGGGAGGGTATCCACCAGTCTCTTCAGCTG	5	0.125	No Hit
GGACACATTACAACATCACCCCACATCTACAGACACCAAAGCCTCCAACC	5	0.125	No Hit
CTTAAATCTTGCTTACACAGAGGACTTGTTAGGATTTTCTCCGTGAGCTT	5	0.125	No Hit
TCTTAATACTGTTCTTTCATTACTTTCAACCCGCAGAAGGAAGGAGTGGG	5	0.125	No Hit
GTTTTTGACTCATGTGGAGGCCCATGAGCAGAGCCCCTACAATCACCACG	5	0.125	No Hit
AGATTGAGCAGTAAAAAGAATTAGAACTCTAAACTTAAGCTAATAGAGTA	5	0.125	No Hit
GCCTTGTAGATTAAAACGAAGGTTACCTAAATAGAATCTAAGTGGCATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0125	0.0
40-41	0.0	0.0	0.0	0.125	0.0
42-43	0.0	0.0	0.0	0.15	0.0
44-45	0.0	0.0	0.0	0.15	0.0
46-47	0.0	0.0	0.0	0.2125	0.0
48-49	0.0	0.0	0.0	0.2875	0.0
50-51	0.0	0.0	0.0	0.35	0.0
52-53	0.0	0.0	0.0	0.425	0.0
54-55	0.0	0.0	0.0	0.575	0.0
56-57	0.0	0.0	0.0	0.7375	0.0
58-59	0.0	0.0	0.0	0.85	0.0
60-61	0.0	0.0	0.0	0.9	0.0
62-63	0.0	0.0	0.0	1.0125	0.0
64-65	0.0	0.0	0.0	1.0750000000000002	0.0
66-67	0.0	0.0	0.0	1.1875	0.0
68-69	0.0	0.0	0.0	1.3375	0.0
70-71	0.0	0.0	0.0	1.4375	0.0
72-73	0.0	0.0	0.0	1.5	0.0
74-75	0.0	0.0	0.0	1.7	0.0
76-77	0.0	0.0	0.0	1.9375	0.0
78-79	0.0	0.0	0.0	2.0374999999999996	0.0
80-81	0.0	0.0	0.0	2.2125	0.0
82-83	0.0	0.0	0.0	2.3875	0.0
84-85	0.0	0.0	0.0	2.4875	0.0
86-87	0.0	0.0	0.0	2.575	0.0
88-89	0.0	0.0	0.0	2.8	0.0
90-91	0.0	0.0	0.0	3.175	0.0
92-93	0.0	0.0	0.0	3.4625	0.0
94-95	0.0	0.0	0.0	3.7125	0.0
96-97	0.0	0.0	0.0	3.925	0.0
98-99	0.0	0.0	0.0	4.15	0.0
100-101	0.0	0.0	0.0	4.512499999999999	0.0
102-103	0.0	0.0	0.0	4.7125	0.0
104-105	0.0	0.0	0.0	4.9	0.0
106-107	0.0	0.0	0.0	5.2	0.0
108-109	0.0	0.0	0.0	5.625	0.0
110-111	0.0	0.0	0.0	5.8375	0.0
112-113	0.0	0.0	0.0	6.1875	0.0
114-115	0.0	0.0	0.0	6.449999999999999	0.0
116-117	0.0	0.0	0.0	6.512499999999999	0.0
118-119	0.0	0.0	0.0	6.875	0.0
120-121	0.0	0.0	0.0	7.25	0.0
122-123	0.0	0.0	0.0	7.675000000000001	0.0
124-125	0.0	0.0	0.0	8.1	0.0
126-127	0.0	0.0	0.0	8.425	0.0
128-129	0.0	0.0	0.0	8.7125	0.0
130-131	0.0	0.0	0.0	9.1375	0.0
132-133	0.0	0.0	0.0	9.7625	0.0
134-135	0.0	0.0	0.0	10.4375	0.0
136-137	0.0	0.0	0.0	10.8875	0.0
138	0.0	0.0	0.0	11.175	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGAGT	10	0.006973645	144.0	4
CATGGGG	15	1.1730364E-4	144.0	4
TACATGG	20	3.687869E-4	108.0	2
ACATGGG	25	8.956223E-4	86.399994	3
GTACATG	35	0.0034045284	61.714283	1
GGGGGGG	20	0.006139246	28.8	140-144
AAAAAAA	40	0.007966741	18.0	140-144
>>END_MODULE
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
Read 2640980 spots for SRR31990354.sra
Written 2640980 spots for SRR31990354.sra
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
Read 2640975 spots for SRR31990354.sra
Written 2640975 spots for SRR31990354.sra
SRR ids: ['SRR31990354.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hezxyins
SRR31990354.sra spots: 21127805
blocks: [[1, 2640975], [2640976, 5281950], [5281951, 7922925], [7922926, 10563900], [10563901, 13204875], [13204876, 15845850], [15845851, 18486825], [18486826, 21127805]]
SRR31990354 file size 7756644
SRR31990354 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR31990354 SRR31990354_1.fastq SRR31990354_2.fastq
Input file:	SRR31990354_1.fastq
Paired file:	SRR31990354_2.fastq
trimmed:	SRR31990354-trimmed-pair1.fastq, SRR31990354-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:32:12 2026 >> started

Sat Apr 25 17:32:41 2026 >> done (29.122s)
21127805 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
21127805 (100.00%) read pairs available; of these:
    1506 ( 0.01%) trimmed read pairs available after processing
21126299 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
141	       1	  0.00%
142	       0	  0.00%
143	       5	  0.00%
144	       3	  0.00%
145	       6	  0.00%
146	       6	  0.00%
147	      20	  0.00%
148	      75	  0.00%
149	    1390	  0.01%
150	21126299	 99.99%
21127805 reads passed initial QC


criterion=sequence-density
sequence-density=8.40
sequence-density-rank=1
fanout-score=37.66
fanout-score-rank=2
prefix-density=9.45
prefix-fanout=33.5
sequence=CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=24
fanout-score=191.26
fanout-score-rank=1
prefix-density=2.58
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=10.06
sequence-density-rank=1
fanout-score=36.33
fanout-score-rank=2
prefix-density=10.98
prefix-fanout=33.3
sequence=CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=20
fanout-score=375.04
fanout-score-rank=1
prefix-density=2.65
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC -y CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA -o SRR31990354 SRR31990354_1.fastq SRR31990354_2.fastq
Input file:	SRR31990354_1.fastq
Paired file:	SRR31990354_2.fastq
trimmed:	SRR31990354-trimmed-pair1.fastq, SRR31990354-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATC
-- paired 3' end adapter sequence (-y):	CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sat Apr 25 17:34:10 2026 >> started

Sat Apr 25 17:34:44 2026 >> done (33.983s)
16902244 read pairs processed; of these:
       5 ( 0.00%) short read pairs filtered out after trimming by size control
       7 ( 0.00%) empty read pairs filtered out after trimming by size control
16902232 (100.00%) read pairs available; of these:
 2274980 (13.46%) trimmed read pairs available after processing
14627252 (86.54%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       2	  0.00%
 19	       5	  0.00%
 20	       1	  0.00%
 21	       9	  0.00%
 22	       6	  0.00%
 23	      20	  0.00%
 24	      10	  0.00%
 25	      40	  0.00%
 26	      31	  0.00%
 27	     118	  0.00%
 28	     301	  0.00%
 29	     405	  0.00%
 30	     539	  0.00%
 31	     833	  0.00%
 32	     766	  0.00%
 33	    1287	  0.01%
 34	    1101	  0.01%
 35	    1401	  0.01%
 36	    2420	  0.01%
 37	    3393	  0.02%
 38	    4181	  0.02%
 39	    3883	  0.02%
 40	    4696	  0.03%
 41	    4149	  0.02%
 42	    4521	  0.03%
 43	    4528	  0.03%
 44	    6587	  0.04%
 45	    5433	  0.03%
 46	    7485	  0.04%
 47	    6653	  0.04%
 48	    7938	  0.05%
 49	    6464	  0.04%
 50	    6911	  0.04%
 51	    6147	  0.04%
 52	    6144	  0.04%
 53	    6675	  0.04%
 54	    6582	  0.04%
 55	   10529	  0.06%
 56	    8174	  0.05%
 57	    7294	  0.04%
 58	    6670	  0.04%
 59	    6461	  0.04%
 60	    7839	  0.05%
 61	    7304	  0.04%
 62	    9612	  0.06%
 63	    9463	  0.06%
 64	    7570	  0.04%
 65	    7404	  0.04%
 66	   10868	  0.06%
 67	    9816	  0.06%
 68	    9177	  0.05%
 69	   12647	  0.07%
 70	   10012	  0.06%
 71	   11778	  0.07%
 72	   10643	  0.06%
 73	    9886	  0.06%
 74	   10373	  0.06%
 75	   11715	  0.07%
 76	   11298	  0.07%
 77	    9544	  0.06%
 78	    9802	  0.06%
 79	    9666	  0.06%
 80	   14214	  0.08%
 81	   19598	  0.12%
 82	   16070	  0.10%
 83	    9785	  0.06%
 84	   11140	  0.07%
 85	   12273	  0.07%
 86	   13749	  0.08%
 87	   16465	  0.10%
 88	   14601	  0.09%
 89	   16200	  0.10%
 90	   15212	  0.09%
 91	   13426	  0.08%
 92	   14316	  0.08%
 93	   17629	  0.10%
 94	   16058	  0.10%
 95	   19594	  0.12%
 96	   17917	  0.11%
 97	   15315	  0.09%
 98	   21382	  0.13%
 99	   21911	  0.13%
100	   19332	  0.11%
101	   19292	  0.11%
102	   18223	  0.11%
103	   21108	  0.12%
104	   22080	  0.13%
105	   25446	  0.15%
106	   24294	  0.14%
107	   24925	  0.15%
108	   24752	  0.15%
109	   22778	  0.13%
110	   22808	  0.13%
111	   24978	  0.15%
112	   25127	  0.15%
113	   25131	  0.15%
114	   24938	  0.15%
115	   21105	  0.12%
116	   20794	  0.12%
117	   23520	  0.14%
118	   21470	  0.13%
119	   28088	  0.17%
120	   25878	  0.15%
121	   29537	  0.17%
122	   29022	  0.17%
123	   31721	  0.19%
124	   30229	  0.18%
125	   30313	  0.18%
126	   32463	  0.19%
127	   33368	  0.20%
128	   25963	  0.15%
129	   35070	  0.21%
130	   39767	  0.24%
131	   35879	  0.21%
132	   37766	  0.22%
133	   39318	  0.23%
134	   35001	  0.21%
135	   43384	  0.26%
136	   40420	  0.24%
137	   32824	  0.19%
138	   38061	  0.23%
139	   39098	  0.23%
140	   47753	  0.28%
141	   43666	  0.26%
142	   45832	  0.27%
143	   42093	  0.25%
144	   46675	  0.28%
145	   36143	  0.21%
146	   45712	  0.27%
147	   50419	  0.30%
148	   52327	  0.31%
149	   56221	  0.33%
150	14626055	 86.53%


criterion=sequence-density
sequence-density=1.23
sequence-density-rank=1
fanout-score=2.14
fanout-score-rank=28
prefix-density=1.29
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=32
fanout-score=185.86
fanout-score-rank=1
prefix-density=2.58
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG


criterion=sequence-density
sequence-density=1.36
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=29
prefix-density=1.38
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=424.02
fanout-score-rank=1
prefix-density=2.65
prefix-fanout=1.2
sequence=GGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTCACCAAAATCACTAACAACCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGACGAACATGAACCCTAATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGG
SRR31990354 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 25 17:36:27
                             Started mapping on |	Apr 25 17:36:27
                                    Finished on |	Apr 25 17:39:02
       Mapping speed, Million of reads per hour |	490.71

                          Number of input reads |	21127793
                      Average input read length |	283
                                    UNIQUE READS:
                   Uniquely mapped reads number |	16639670
                        Uniquely mapped reads % |	78.76%
                          Average mapped length |	285.51
                       Number of splices: Total |	16975822
            Number of splices: Annotated (sjdb) |	16886160
                       Number of splices: GT/AG |	16915008
                       Number of splices: GC/AG |	26227
                       Number of splices: AT/AC |	4198
               Number of splices: Non-canonical |	30389
                      Mismatch rate per base, % |	0.51%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.25
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1807933
             % of reads mapped to multiple loci |	8.56%
        Number of reads mapped to too many loci |	41080
             % of reads mapped to too many loci |	0.19%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.41%
                     % of reads unmapped: other |	0.08%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2680190	2680190	2680190
N_multimapping	1807933	1807933	1807933
N_noFeature	1384879	8355078	8503632
N_ambiguous	1516341	187605	186802
UnstrandedReadsAssigned:13738450 PositiveStrandReadsAssigned:8096987 NegativeStrandReadsAssigned:7949236
Dataset is classified unstranded
MeadianReadLen=142 20thPercentileLength=142 echo kmer=137
SRR31990354 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR31990354-trimmed-pair1.fastq
                             SRR31990354-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 21,127,793 reads, 17,401,813 reads pseudoaligned
[quant] estimated average fragment length: 232.034
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,127 rounds

 109283 SRR31990354.ke.tsv
  52636 SRR31990354.se.tsv
 161919 total
==> SRR31990354.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	16	0	0
ENSMUST00000196221.1	9	9	0	0
ENSMUST00000179664.1	11	11	0	0
ENSMUST00000178537.1	12	12	0	0
ENSMUST00000178862.1	14	14	0	0
ENSMUST00000179520.1	11	11	0	0
ENSMUST00000179883.1	16	16	0	0
ENSMUST00000195858.1	10	10	0	0
ENSMUST00000179932.1	12	12	0	0

==> SRR31990354.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	4
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	1
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR31990354 completed mapping pipeline successfully
