Starting /dee2/code/volunteer_pipeline.sh SRR32233443
    current disk space = 819495996
    free memory = 198663788 
SRR32233443 SRAfilesize
17a991185140820efd4f88fbe69b1bae  SRR32233443.sra
SRR32233443.sra file validated
SRR32233443 is paired end
SRR32233443 is conventional basespace
SRR32233443 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4935	37.0	37.0	37.0	37.0	37.0
2	36.429	37.0	37.0	37.0	37.0	37.0
3	36.422	37.0	37.0	37.0	37.0	37.0
4	36.537	37.0	37.0	37.0	37.0	37.0
5	36.5725	37.0	37.0	37.0	37.0	37.0
6	36.48	37.0	37.0	37.0	37.0	37.0
7	36.509	37.0	37.0	37.0	37.0	37.0
8	36.617	37.0	37.0	37.0	37.0	37.0
9	36.4965	37.0	37.0	37.0	37.0	37.0
10-14	36.516799999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.483700000000006	37.0	37.0	37.0	37.0	37.0
20-24	36.4607	37.0	37.0	37.0	37.0	37.0
25-29	36.4508	37.0	37.0	37.0	37.0	37.0
30-34	36.4126	37.0	37.0	37.0	37.0	37.0
35-39	36.3742	37.0	37.0	37.0	37.0	37.0
40-44	36.365500000000004	37.0	37.0	37.0	37.0	37.0
45-49	36.3532	37.0	37.0	37.0	37.0	37.0
50-54	36.2611	37.0	37.0	37.0	37.0	37.0
55-59	36.264300000000006	37.0	37.0	37.0	37.0	37.0
60-64	36.251099999999994	37.0	37.0	37.0	37.0	37.0
65-69	36.188	37.0	37.0	37.0	37.0	37.0
70-74	36.149300000000004	37.0	37.0	37.0	37.0	37.0
75-79	36.1497	37.0	37.0	37.0	37.0	37.0
80-84	36.1056	37.0	37.0	37.0	37.0	37.0
85-89	36.0887	37.0	37.0	37.0	37.0	37.0
90-94	36.047900000000006	37.0	37.0	37.0	37.0	37.0
95-99	35.9589	37.0	37.0	37.0	37.0	37.0
100-104	35.9944	37.0	37.0	37.0	37.0	37.0
105-109	35.8969	37.0	37.0	37.0	37.0	37.0
110-114	35.755900000000004	37.0	37.0	37.0	37.0	37.0
115-119	35.7864	37.0	37.0	37.0	37.0	37.0
120-124	35.59589999999999	37.0	37.0	37.0	37.0	37.0
125-129	35.568599999999996	37.0	37.0	37.0	37.0	37.0
130-134	35.4718	37.0	37.0	37.0	37.0	37.0
135-139	35.3957	37.0	37.0	37.0	37.0	37.0
140-144	35.19915	37.0	37.0	37.0	27.4	37.0
145-149	35.0243	37.0	37.0	37.0	25.0	37.0
150	35.0015	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	0.0
22	0.0
23	1.0
24	2.0
25	3.0
26	4.0
27	9.0
28	14.0
29	14.0
30	37.0
31	42.0
32	59.0
33	100.0
34	140.0
35	459.0
36	2972.0
37	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.375000000000004	11.200000000000001	13.850000000000001	44.574999999999996
2	18.8344172086043	14.682341170585293	37.41870935467734	29.064532266133064
3	20.075000000000003	17.125	28.15	34.65
4	21.25	25.874999999999996	26.125	26.75
5	26.25	28.249999999999996	24.349999999999998	21.15
6	20.4	32.75	29.65	17.2
7	14.825	22.400000000000002	44.525	18.25
8	13.525	26.075	37.574999999999996	22.825
9	15.45	27.650000000000002	34.949999999999996	21.95
10-14	18.47	31.900000000000002	28.32	21.310000000000002
15-19	20.23	30.42	28.915000000000003	20.435
20-24	18.875	31.730000000000004	29.315	20.080000000000002
25-29	19.25	29.270000000000003	29.599999999999998	21.88
30-34	19.29	30.464999999999996	27.85	22.395
35-39	19.49	28.93	30.3	21.279999999999998
40-44	18.94	29.349999999999998	29.175	22.535
45-49	19.919999999999998	28.785	29.509999999999998	21.785
50-54	19.665	30.354999999999997	29.49	20.49
55-59	18.755	31.25	28.52	21.475
60-64	20.935000000000002	29.549999999999997	29.080000000000002	20.435
65-69	19.585	28.67	29.18	22.564999999999998
70-74	19.55	28.804999999999996	29.13	22.515
75-79	19.98	29.69	29.885	20.445
80-84	18.87	29.759999999999998	29.525000000000002	21.845
85-89	19.105	29.294999999999998	30.464999999999996	21.135
90-94	21.3	28.87	29.615000000000002	20.215
95-99	19.1	29.675	29.720000000000002	21.505
100-104	18.925	28.76	31.06	21.255
105-109	18.975	27.775	30.755	22.495
110-114	20.305	29.43	29.38	20.885
115-119	18.955	28.405	31.2	21.44
120-124	20.16	27.85	31.39	20.599999999999998
125-129	18.6	29.145	32.06	20.195
130-134	20.785	28.935	29.595	20.685000000000002
135-139	18.81	28.835	29.365000000000002	22.99
140-144	18.64593229661483	29.53147657382869	30.441522076103805	21.381069053452674
145-149	20.41	29.73	28.415000000000003	21.445
150	20.200000000000003	30.7	27.200000000000003	21.9
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	0.0
18	0.0
19	1.0
20	1.5
21	1.5
22	4.0
23	10.5
24	19.0
25	28.0
26	54.5
27	104.0
28	137.5
29	132.0
30	136.0
31	108.0
32	61.5
33	63.0
34	95.5
35	99.0
36	115.0
37	147.0
38	147.5
39	168.0
40	188.0
41	194.0
42	163.5
43	167.0
44	160.0
45	108.0
46	132.0
47	163.0
48	151.5
49	120.5
50	101.0
51	122.5
52	101.5
53	79.5
54	70.5
55	48.5
56	48.0
57	34.5
58	22.0
59	24.0
60	25.5
61	16.0
62	4.5
63	4.0
64	5.5
65	5.0
66	5.0
67	7.5
68	14.5
69	11.5
70	3.0
71	9.0
72	14.5
73	9.5
74	8.0
75	7.0
76	4.0
77	5.5
78	4.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.005
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.646408839779	24.275
2	23.370165745856355	21.15
3	9.613259668508286	13.05
4	4.198895027624309	7.6
5	3.314917127071823	7.5
6	1.6022099447513811	4.35
7	1.1049723756906076	3.5000000000000004
8	0.6077348066298343	2.1999999999999997
9	0.44198895027624313	1.7999999999999998
>10	2.0994475138121547	14.575
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	28	0.7000000000000001	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	24	0.6	No Hit
CTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAACC	22	0.5499999999999999	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	22	0.5499999999999999	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	21	0.525	No Hit
GCTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAA	20	0.5	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	20	0.5	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	20	0.5	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	20	0.5	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	19	0.475	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	19	0.475	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	18	0.44999999999999996	No Hit
GCTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCTGGTCTTCATGG	17	0.42500000000000004	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	17	0.42500000000000004	No Hit
CCCCCAAGCAGCTAACTTGGCGATAATTCTTGCTGCCATATGAACAGTAA	17	0.42500000000000004	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	16	0.4	No Hit
CCACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATG	16	0.4	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	15	0.375	No Hit
CGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAA	15	0.375	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	14	0.35000000000000003	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	14	0.35000000000000003	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	14	0.35000000000000003	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	13	0.325	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	13	0.325	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	13	0.325	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	12	0.3	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	11	0.27499999999999997	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	11	0.27499999999999997	No Hit
AGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGT	11	0.27499999999999997	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	11	0.27499999999999997	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	10	0.25	No Hit
CTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAA	10	0.25	No Hit
CTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAA	10	0.25	No Hit
CAGAAGTTTTGTCAAGATTTTCAAGAGTTCATAGTTCTTCTCATTCAACC	10	0.25	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	10	0.25	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	10	0.25	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	10	0.25	No Hit
GTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTA	10	0.25	No Hit
CTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATGAA	9	0.22499999999999998	No Hit
CTCCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACA	9	0.22499999999999998	No Hit
CTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAAC	9	0.22499999999999998	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	9	0.22499999999999998	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	9	0.22499999999999998	No Hit
CTTCCATCAGTTCTTTCCCCCAAGCAGCTAACTTGGCGATAATTCTTGCT	9	0.22499999999999998	No Hit
CTCCATTCCAATCTTCCAGATTTAAGTTCTGAACTGTATTCATCAAATGA	9	0.22499999999999998	No Hit
CCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGA	9	0.22499999999999998	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	8	0.2	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	8	0.2	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	8	0.2	No Hit
CGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATT	8	0.2	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	8	0.2	No Hit
TCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	8	0.2	No Hit
CAAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCAGATTT	8	0.2	No Hit
CCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTCA	8	0.2	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	8	0.2	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	8	0.2	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	8	0.2	No Hit
GGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTAAAA	7	0.17500000000000002	No Hit
GCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGTG	7	0.17500000000000002	No Hit
TAAAGACTGAGGTTCTCACTCCACTGGTCCATGGGAAGTGCAGAAAGGCT	7	0.17500000000000002	No Hit
CGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTG	7	0.17500000000000002	No Hit
CTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCTGTATCTCAAA	7	0.17500000000000002	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	7	0.17500000000000002	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	7	0.17500000000000002	No Hit
TTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAAATA	7	0.17500000000000002	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	7	0.17500000000000002	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	7	0.17500000000000002	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	7	0.17500000000000002	No Hit
GCTCCATTCCAATCTTCCAGATTTAAGTTCTGAACTGTATTCATCAAATG	7	0.17500000000000002	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	7	0.17500000000000002	No Hit
CAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATCATTA	7	0.17500000000000002	No Hit
GTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTA	7	0.17500000000000002	No Hit
AGCACTACGAGTTTGCACCTGTGTATAAGTGCAGTTCTTCTTTTTACATT	7	0.17500000000000002	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	7	0.17500000000000002	No Hit
GTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCA	7	0.17500000000000002	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	7	0.17500000000000002	No Hit
GACTATTTTAACCTTATTACTTTTAAACTAATTCTTTCAAACTGTACTTT	7	0.17500000000000002	No Hit
CCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATCT	6	0.15	No Hit
CTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAAAT	6	0.15	No Hit
AGCTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAA	6	0.15	No Hit
GTTAGTATGTACTGCACTGTCTGTTCTTTGGAGATGTGCGTCATCAGATT	6	0.15	No Hit
CACATTTAAAAAGTAAAACTTAATTGTGAAATATGTTTTGTAAAACTATT	6	0.15	No Hit
CCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACACT	6	0.15	No Hit
GCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAG	6	0.15	No Hit
GCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAAT	6	0.15	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	6	0.15	No Hit
TGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATG	6	0.15	No Hit
CTTTTCCTTTTAACATGTTTAGGTACATACCAATGAGAAAAGCAATTTAT	6	0.15	No Hit
CCTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCACTG	6	0.15	No Hit
CTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGTTCTTCTTC	6	0.15	No Hit
GTAATTTTCAAATGATGTAATAAAACTGTTTGTTATCTTATTTTGCTGAA	6	0.15	No Hit
CAGTAATTAAGAGTAAAGTAATATGCTAGTTCAAATTGTGTGCACAACTT	6	0.15	No Hit
GCAGTATCTAATTTTATACAATTTAACAAGCAAACACTTTTATTCTCCCA	6	0.15	No Hit
GTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAAA	6	0.15	No Hit
GAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAA	6	0.15	No Hit
TGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCG	6	0.15	No Hit
GCTTGACATCATCATGTAAGAAAAATTTCAGCACAAAAGATTCAGTCTAA	6	0.15	No Hit
ATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCCCATT	6	0.15	No Hit
CTCAAACTCAGAAATCCGCCTGCCTCTGCCTCCCGAGTTCTGGGATTAAA	6	0.15	No Hit
TCTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGA	6	0.15	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	6	0.15	No Hit
AAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCAGATTTA	6	0.15	No Hit
AAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATG	6	0.15	No Hit
CAAGTTTTTCCAAAAGAAATTTGATGTCTTCACTGATATCTTCATCATCA	6	0.15	No Hit
CAAGATATTCCCAGTTGTGCACCATGAGCTTCTGCACGGCCAGAAGAGCA	6	0.15	No Hit
CTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATTT	6	0.15	No Hit
GATTGTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAG	5	0.125	No Hit
CCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCC	5	0.125	No Hit
AGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAA	5	0.125	No Hit
CTATTTTAACCTTATTACTTTTAAACTAATTCTTTCAAACTGTACTTTAT	5	0.125	No Hit
CTCCAGAAGTTTTGTCAAGATTTTCAAGAGTTCATAGTTCTTCTCATTCA	5	0.125	No Hit
CTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCTGTATAGC	5	0.125	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	5	0.125	No Hit
GTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACAT	5	0.125	No Hit
TCCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAAT	5	0.125	No Hit
CCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAG	5	0.125	No Hit
CCATGGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTT	5	0.125	No Hit
CCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAG	5	0.125	No Hit
CTCCCTCCACCCGCTTCATGCGCTTCACCTGCTTGCGCCGCCGCGGCCGG	5	0.125	No Hit
GGCGGCTCTACGGACACTGCATAGTCCGAGACTGGAGCGTAAGTGTAGGT	5	0.125	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	5	0.125	No Hit
GCCCAGTTGTGCACCATGAGCTTCTGCACGGCCAGAAGAGCATTGTAGCG	5	0.125	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	5	0.125	No Hit
CTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATA	5	0.125	No Hit
GTTCGTCTTTGGCCCACACCATAAAGGCGTTCATCGGCCGCCGGATGCGA	5	0.125	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	5	0.125	No Hit
GTGAACAACAAAGACCAACCCATGAGATGTATGTTGTTTACAAATTTCAC	5	0.125	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	5	0.125	No Hit
CTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCTG	5	0.125	No Hit
GTAACTCTAAAAATTTATATTATGCCTTTTCCTTTTAACATGTTTAGGTA	5	0.125	No Hit
CTCCCGAGTTCTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAA	5	0.125	No Hit
GACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCT	5	0.125	No Hit
CTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAAATA	5	0.125	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	5	0.125	No Hit
ATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTCATTGAC	5	0.125	No Hit
GTACATACCAATGAGAAAAGCAATTTATCTTTATGCATTAACAGATCAAC	5	0.125	No Hit
GCTCCCTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCA	5	0.125	No Hit
ATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTT	5	0.125	No Hit
GAAAAGATGAAACATATGTATCTCGAGCATTTGTCTCATCCTTTCTGCTG	5	0.125	No Hit
AGAAGTTTTGTCAAGATTTTCAAGAGTTCATAGTTCTTCTCATTCAACCT	5	0.125	No Hit
GTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACTGG	5	0.125	No Hit
GGCAATATTAGTTTTCTACGGAAAATCAAAAAAGAACCTTGTTTTTTCTT	5	0.125	No Hit
CACGCATGACAAAATTTCTCTAGATCAATACACTTTCAAGAAATGGAATA	5	0.125	No Hit
CCCGAGTTCTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATC	5	0.125	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	5	0.125	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	5	0.125	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	5	0.125	No Hit
CCCAGCCATAGCTGCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGA	5	0.125	No Hit
CACCAATCAGGAGCTATAGAAGCCGCTCCACCACCTAACCTGGGCCAGTC	5	0.125	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	5	0.125	No Hit
CTCGCCTTTCACCTTTACATCCCCGAGGGGGCTCAGGGACTCGGCCCAGG	5	0.125	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	5	0.125	No Hit
CTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTC	5	0.125	No Hit
GTGGGATCAAGACTTTTGGAAGAAAAGACAAACAGGAAAGAGTTATATCT	5	0.125	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	5	0.125	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	5	0.125	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	5	0.125	No Hit
GTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGT	5	0.125	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	5	0.125	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	5	0.125	No Hit
CTCAGGTCGGGTCGGCAACCGTCAAATGTCGGGGTAGTTGCAATAGTAGA	5	0.125	No Hit
GCACTGTCTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTA	5	0.125	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	5	0.125	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	5	0.125	No Hit
GTCCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAG	5	0.125	No Hit
GTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAAGT	10	0.006973645	144.0	7
GTTCAAG	10	0.006973645	144.0	6
>>END_MODULE
SRR32233443 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233443_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.99525	37.0	37.0	37.0	37.0	37.0
2	36.1435	37.0	37.0	37.0	37.0	37.0
3	36.116	37.0	37.0	37.0	37.0	37.0
4	36.114	37.0	37.0	37.0	37.0	37.0
5	35.9975	37.0	37.0	37.0	37.0	37.0
6	36.0745	37.0	37.0	37.0	37.0	37.0
7	36.1175	37.0	37.0	37.0	37.0	37.0
8	36.1245	37.0	37.0	37.0	37.0	37.0
9	36.036	37.0	37.0	37.0	37.0	37.0
10-14	36.1612	37.0	37.0	37.0	37.0	37.0
15-19	36.1149	37.0	37.0	37.0	37.0	37.0
20-24	36.115899999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.077000000000005	37.0	37.0	37.0	37.0	37.0
30-34	35.9174	37.0	37.0	37.0	37.0	37.0
35-39	35.966699999999996	37.0	37.0	37.0	37.0	37.0
40-44	35.863200000000006	37.0	37.0	37.0	37.0	37.0
45-49	35.8639	37.0	37.0	37.0	37.0	37.0
50-54	35.74765	37.0	37.0	37.0	37.0	37.0
55-59	35.69789999999999	37.0	37.0	37.0	37.0	37.0
60-64	35.60975	37.0	37.0	37.0	37.0	37.0
65-69	35.6922	37.0	37.0	37.0	37.0	37.0
70-74	35.518899999999995	37.0	37.0	37.0	37.0	37.0
75-79	35.399699999999996	37.0	37.0	37.0	37.0	37.0
80-84	35.3009	37.0	37.0	37.0	29.8	37.0
85-89	35.2743	37.0	37.0	37.0	27.4	37.0
90-94	35.1898	37.0	37.0	37.0	27.4	37.0
95-99	35.015699999999995	37.0	37.0	37.0	25.0	37.0
100-104	34.977	37.0	37.0	37.0	25.0	37.0
105-109	34.9777	37.0	37.0	37.0	25.0	37.0
110-114	34.6812	37.0	37.0	37.0	25.0	37.0
115-119	34.6216	37.0	37.0	37.0	25.0	37.0
120-124	34.401300000000006	37.0	37.0	37.0	25.0	37.0
125-129	34.218399999999995	37.0	37.0	37.0	25.0	37.0
130-134	34.030899999999995	37.0	37.0	37.0	25.0	37.0
135-139	33.98440000000001	37.0	37.0	37.0	25.0	37.0
140-144	33.65410000000001	37.0	37.0	37.0	25.0	37.0
145-149	33.7603	37.0	37.0	37.0	25.0	37.0
150	33.399	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	2.0
24	14.0
25	8.0
26	5.0
27	13.0
28	14.0
29	32.0
30	42.0
31	69.0
32	121.0
33	237.0
34	480.0
35	1371.0
36	1566.0
37	22.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.40710177544386	22.80570142535634	18.37959489872468	30.407601900475118
2	21.85	35.925000000000004	31.85	10.375
3	21.349999999999998	33.575	23.200000000000003	21.875
4	21.9	38.725	21.475	17.9
5	23.075000000000003	38.675	21.9	16.35
6	18.85	40.75	24.0	16.400000000000002
7	19.625	19.975	38.7	21.7
8	20.05	24.474999999999998	31.25	24.224999999999998
9	23.925	23.25	30.099999999999998	22.725
10-14	23.89	29.354999999999997	25.705	21.05
15-19	22.16	29.445	27.52	20.875
20-24	22.025	30.455	27.1	20.419999999999998
25-29	23.115	30.064999999999998	26.805	20.015
30-34	22.32	29.825000000000003	27.839999999999996	20.015
35-39	22.127212721272127	29.88798879887989	28.0978097809781	19.886988698869885
40-44	22.495	29.775000000000002	27.18	20.549999999999997
45-49	22.175	30.055	27.01	20.76
50-54	21.906095304765238	30.606530326516324	27.51137556877844	19.975998799939997
55-59	21.435000000000002	28.98	28.439999999999998	21.145
60-64	21.801090054502726	30.64653232661633	27.621381069053452	19.930996549827494
65-69	22.35	28.985	28.175	20.49
70-74	21.43	30.275000000000002	28.799999999999997	19.495
75-79	22.75	29.21	27.73	20.31
80-84	22.770000000000003	30.59	27.105	19.535
85-89	22.355	30.214999999999996	27.715	19.715
90-94	21.55	31.135	27.505000000000003	19.81
95-99	21.465	29.68	29.065	19.79
100-104	22.1	29.615000000000002	27.96	20.325
105-109	22.595000000000002	30.34	26.915	20.150000000000002
110-114	21.33	29.92	28.904999999999998	19.845
115-119	21.98	29.785	28.625	19.61
120-124	21.015	30.014999999999997	29.599999999999998	19.37
125-129	21.485000000000003	29.21	29.404999999999998	19.900000000000002
130-134	22.045	30.130000000000003	28.595	19.23
135-139	21.535	30.395	28.43	19.64
140-144	22.245	30.2	28.305000000000003	19.25
145-149	21.34	30.14	28.98	19.54
150	20.925	30.7	29.625	18.75
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.0
14	0.0
15	0.0
16	1.5
17	1.5
18	0.0
19	1.5
20	2.0
21	3.5
22	10.0
23	26.0
24	45.0
25	49.5
26	46.5
27	69.0
28	81.5
29	81.0
30	75.0
31	69.0
32	99.0
33	112.5
34	103.5
35	95.0
36	105.0
37	121.0
38	149.0
39	188.0
40	208.0
41	216.5
42	203.0
43	177.5
44	146.5
45	127.0
46	127.0
47	133.5
48	125.0
49	93.5
50	83.0
51	87.5
52	90.0
53	78.0
54	57.5
55	42.0
56	35.5
57	37.5
58	31.5
59	28.0
60	26.5
61	20.5
62	13.5
63	13.5
64	14.0
65	20.0
66	25.0
67	24.5
68	24.0
69	20.0
70	20.5
71	34.0
72	36.0
73	19.5
74	11.0
75	6.5
76	1.5
77	2.0
78	2.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.01
40-44	0.0
45-49	0.0
50-54	0.005
55-59	0.0
60-64	0.005
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.00000000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.48214285714286	32.75
2	22.5	25.2
3	9.955357142857142	16.725
4	4.642857142857143	10.4
5	2.5892857142857144	7.249999999999999
6	0.8035714285714285	2.7
7	0.26785714285714285	1.05
8	0.3125	1.4000000000000001
9	0.13392857142857142	0.675
>10	0.3125	1.8499999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	13	0.325	No Hit
CAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATT	11	0.27499999999999997	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	10	0.25	No Hit
CGGAAAGAGAAACTCGCCAAGAGTATGCTCTGGCTATGATTCAATGCAAA	10	0.25	No Hit
AAAAATTTTGTTGATCTGTTAATGCATAAAGATAAATTGCTTTTCTCATT	10	0.25	No Hit
CAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAG	10	0.25	No Hit
CAGACATCCTTCAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTT	10	0.25	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	9	0.22499999999999998	No Hit
GGACAAGCAAGAGATGCTTCAAACTGAAGGCAGCCAGTGTGCTAAAACAT	9	0.22499999999999998	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	9	0.22499999999999998	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	8	0.2	No Hit
GCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAAT	8	0.2	No Hit
TGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCTA	8	0.2	No Hit
AAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCT	8	0.2	No Hit
CAGAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGGAACAATCTCTTC	8	0.2	No Hit
GTGGTTTTCAACTTCAATACCAAATGATTTTTTCAATATGGCTCCTGGCA	8	0.2	No Hit
CACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTA	8	0.2	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	7	0.17500000000000002	No Hit
GAAACATTCAAAGTCATAAAGTTTAGATGACCAAGATGGACATTCGAGGT	7	0.17500000000000002	No Hit
AAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGT	7	0.17500000000000002	No Hit
ATTTTCTGTACATGTTATGCTTAAAACCAAATACTGTTCAAGATGAGCAA	7	0.17500000000000002	No Hit
GAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCA	7	0.17500000000000002	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	7	0.17500000000000002	No Hit
AAAGGATTGGTCTGCCGGGCGTGGTGGCCCACACCTTTAATCCCAGAACT	6	0.15	No Hit
CGCCAAGTTAGCTGCTTGGGGGAAAGAACTGATGGAAGGCAGTGACTTAA	6	0.15	No Hit
GGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTT	6	0.15	No Hit
TGTACACAAGTCTGAGAAATTTTGGAGAGAAAATGCCGTGAGGTTGAATG	6	0.15	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	6	0.15	No Hit
GAAGAACTATGAACTCTTGAAAATCTTGACAAAACTTCTGGAGGTATCAG	6	0.15	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	6	0.15	No Hit
CATTCTTGCAGCATTCCGGAATTTCTTAGAAAAATCAACGGAAAGAGAAA	6	0.15	No Hit
GTAAAAACCGTTGCACAAGCTTTGCACTCTTCTATGCTCTGCTTTGTAGA	6	0.15	No Hit
TTCAATACCAAATGATTTTTTCAATATGGCTCCTGGCATTCAGTCCTCAA	6	0.15	No Hit
CTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCA	6	0.15	No Hit
AAACATGTTAAAAGGAAAAGGCATAATATAAATTTTTAGAGTTACCAAAT	6	0.15	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	6	0.15	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	6	0.15	No Hit
GTGGATGCTGCTGTCCCAACTAACATCATTGCGGCCAAGGCTGCTGAAGT	6	0.15	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	6	0.15	No Hit
GATTTTTTCAATATGGCTCCTGGCATTCAGTCCTCAAATGTGTGAGCACC	6	0.15	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	6	0.15	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	5	0.125	No Hit
AAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATAT	5	0.125	No Hit
AGCAGATGGAGTGAACTGTATAATGGGCGTTTTGAGTAACAAGTGTGGTT	5	0.125	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	5	0.125	No Hit
AAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATC	5	0.125	No Hit
AAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAA	5	0.125	No Hit
AAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAAGA	5	0.125	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	5	0.125	No Hit
GTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGC	5	0.125	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	5	0.125	No Hit
GCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAAGAGG	5	0.125	No Hit
CTCGTAGTGCTGATGAACCAATGACAACATTTGTTGTATGTAATGAATGT	5	0.125	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	5	0.125	No Hit
TTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCA	5	0.125	No Hit
GTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTT	5	0.125	No Hit
CTGGACCATTAAGAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCA	5	0.125	No Hit
TGATACTATAATAGCTAAGAATCAGATGGTATTACATTATCCCTAATAAT	5	0.125	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	5	0.125	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	5	0.125	No Hit
ATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAA	5	0.125	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	5	0.125	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	5	0.125	No Hit
CGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCC	5	0.125	No Hit
GAAATTTTGGAGAGAAAATGCCGTGAGGTTGAATGAGAAGAACTATGAAC	5	0.125	No Hit
GAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAA	5	0.125	No Hit
CACAAACAGCGGGGTGTTCAAATGTGATATAATTGTTCTGAGAAAAATTC	5	0.125	No Hit
GGCAACTCGTTTCCTTGCAAGTGAAATTTGTAAACAACATACATCTCATG	5	0.125	No Hit
CACAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATGTCCGGCAT	5	0.125	No Hit
GTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGT	5	0.125	No Hit
CACAGAGAAACCCTGTCTCGGAAACAAACAAACAAACAAACCGAAACACA	5	0.125	No Hit
CTCGGAAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCC	5	0.125	No Hit
AAGACATCAAATTTCTTTTGGAAAAACTTGGTGAGAGTGTCCAGGACCTT	5	0.125	No Hit
AGCCCGGATGCGGGATACGCCAGTGACGACCAGAGCCAGCCCCGGAGCGC	5	0.125	No Hit
TTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTC	5	0.125	No Hit
CGAGGTGGTTCGCATTGCCAAGAAGATGGACAAAATGGTGCAGAAAAAGA	5	0.125	No Hit
TGGACCATTAAGAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAG	5	0.125	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	5	0.125	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	5	0.125	No Hit
ATCCTTCAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTTGCAGC	5	0.125	No Hit
GTAAGGCTGGCTCAGGTTAGGTGGTGGAGCGGCTTCTATAGCTCCTGATG	5	0.125	No Hit
CAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCC	5	0.125	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	5	0.125	No Hit
CTCAAATGTGTGAGCACCTTCGTCGCTATAATATCATTCCTGTTCTATCA	5	0.125	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	5	0.125	No Hit
GCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAAT	5	0.125	No Hit
TTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAACT	5	0.125	No Hit
TGAATGAGAAGAACTATGAACTCTTGAAAATCTTGACAAAACTTCTGGAG	5	0.125	No Hit
ATTTGATTTTAAATCATCAGCATGTTCAAAATACTGGTGAAAATTATTTA	5	0.125	No Hit
CAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCAAC	5	0.125	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	5	0.125	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	5	0.125	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	5	0.125	No Hit
CATTCGAGGTGCTGTGGATGCTGCTGTCCCAACTAACATCATTGCGGCCA	5	0.125	No Hit
GAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAG	5	0.125	No Hit
GTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAATAAAT	5	0.125	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	5	0.125	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	5	0.125	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCAAT	10	0.0069863307	143.91249	6
CAATTGG	10	0.0069863307	143.91249	9
GCAATTG	10	0.0069863307	143.91249	8
>>END_MODULE
2026-04-17T04:12:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:49 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:57 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:12:59 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
2026-04-17T04:13:04 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:07 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:09 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:10 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
2026-04-17T04:13:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
2026-04-17T04:13:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
2026-04-17T04:13:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:13:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
2026-04-17T04:13:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
Read 1730622 spots for SRR32233443.sra
Written 1730622 spots for SRR32233443.sra
SRR ids: ['SRR32233443.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_jtu0oyau
SRR32233443.sra spots: 13844976
blocks: [[1, 1730622], [1730623, 3461244], [3461245, 5191866], [5191867, 6922488], [6922489, 8653110], [8653111, 10383732], [10383733, 12114354], [12114355, 13844976]]
SRR32233443 file size 4656392
SRR32233443 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233443 SRR32233443_1.fastq SRR32233443_2.fastq
Input file:	SRR32233443_1.fastq
Paired file:	SRR32233443_2.fastq
trimmed:	SRR32233443-trimmed-pair1.fastq, SRR32233443-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:13:44 2026 >> started

Fri Apr 17 04:14:15 2026 >> done (31.176s)
13844976 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       3 ( 0.00%) empty read pairs filtered out after trimming by size control
13844973 (100.00%) read pairs available; of these:
  108125 ( 0.78%) trimmed read pairs available after processing
13736848 (99.22%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 23	       1	  0.00%
 24	       1	  0.00%
 25	       1	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       1	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       1	  0.00%
 40	       0	  0.00%
 41	       1	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       1	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       1	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       1	  0.00%
 69	       6	  0.00%
 70	       3	  0.00%
 71	       5	  0.00%
 72	       5	  0.00%
 73	       5	  0.00%
 74	       2	  0.00%
 75	       6	  0.00%
 76	      10	  0.00%
 77	      19	  0.00%
 78	       6	  0.00%
 79	      11	  0.00%
 80	       8	  0.00%
 81	      15	  0.00%
 82	      15	  0.00%
 83	      14	  0.00%
 84	      14	  0.00%
 85	      16	  0.00%
 86	      32	  0.00%
 87	      19	  0.00%
 88	      17	  0.00%
 89	      25	  0.00%
 90	      31	  0.00%
 91	      25	  0.00%
 92	      30	  0.00%
 93	      29	  0.00%
 94	      44	  0.00%
 95	      42	  0.00%
 96	      39	  0.00%
 97	      47	  0.00%
 98	      45	  0.00%
 99	      66	  0.00%
100	      56	  0.00%
101	      68	  0.00%
102	      60	  0.00%
103	      56	  0.00%
104	      73	  0.00%
105	      68	  0.00%
106	      93	  0.00%
107	      65	  0.00%
108	      89	  0.00%
109	      80	  0.00%
110	      94	  0.00%
111	      75	  0.00%
112	      91	  0.00%
113	     120	  0.00%
114	     105	  0.00%
115	      98	  0.00%
116	     107	  0.00%
117	     121	  0.00%
118	     134	  0.00%
119	     116	  0.00%
120	     132	  0.00%
121	     157	  0.00%
122	     143	  0.00%
123	     175	  0.00%
124	     158	  0.00%
125	     174	  0.00%
126	     169	  0.00%
127	     172	  0.00%
128	     201	  0.00%
129	     210	  0.00%
130	     205	  0.00%
131	     258	  0.00%
132	     217	  0.00%
133	     268	  0.00%
134	     221	  0.00%
135	     238	  0.00%
136	     277	  0.00%
137	     293	  0.00%
138	     290	  0.00%
139	     321	  0.00%
140	     341	  0.00%
141	   10161	  0.07%
142	   10395	  0.08%
143	   10503	  0.08%
144	   11132	  0.08%
145	   11268	  0.08%
146	   11535	  0.08%
147	   11761	  0.08%
148	   12041	  0.09%
149	   12279	  0.09%
150	13736848	 99.22%
13844973 reads passed initial QC


criterion=sequence-density
sequence-density=2.52
sequence-density-rank=1
fanout-score=2.81
fanout-score-rank=35
prefix-density=7.07
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=12
fanout-score=67.02
fanout-score-rank=1
prefix-density=7.16
prefix-fanout=1.0
sequence=GTTTTGAAGCACAGGCCTCTCAA


criterion=sequence-density
sequence-density=6.56
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=33
prefix-density=6.53
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.09
sequence-density-rank=14
fanout-score=40.40
fanout-score-rank=1
prefix-density=3.75
prefix-fanout=1.0
sequence=TGGATCAACAACTCTCCTAGGCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT -y CTAATAATTGTTT -o SRR32233443 SRR32233443_1.fastq SRR32233443_2.fastq
Input file:	SRR32233443_1.fastq
Paired file:	SRR32233443_2.fastq
trimmed:	SRR32233443-trimmed-pair1.fastq, SRR32233443-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATA
-- paired 3' end adapter sequence (-y):	CTAATAATTGTTT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:15:34 2026 >> started

Fri Apr 17 04:15:48 2026 >> done (13.646s)
8306984 read pairs processed; of these:
    174 ( 0.00%) short read pairs filtered out after trimming by size control
   1762 ( 0.02%) empty read pairs filtered out after trimming by size control
8305048 (99.98%) read pairs available; of these:
     11 ( 0.00%) trimmed read pairs available after processing
8305037 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 23	      1	  0.00%
 24	      1	  0.00%
 25	      0	  0.00%
 26	      0	  0.00%
 27	      0	  0.00%
 28	      0	  0.00%
 29	      0	  0.00%
 30	      1	  0.00%
 31	      0	  0.00%
 32	      0	  0.00%
 33	      0	  0.00%
 34	      0	  0.00%
 35	      0	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      1	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      1	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      5	  0.00%
 70	      3	  0.00%
 71	      3	  0.00%
 72	      5	  0.00%
 73	      2	  0.00%
 74	      2	  0.00%
 75	      4	  0.00%
 76	      5	  0.00%
 77	     13	  0.00%
 78	      3	  0.00%
 79	      5	  0.00%
 80	      7	  0.00%
 81	      5	  0.00%
 82	     10	  0.00%
 83	     10	  0.00%
 84	      3	  0.00%
 85	      6	  0.00%
 86	     19	  0.00%
 87	      8	  0.00%
 88	      8	  0.00%
 89	     17	  0.00%
 90	     15	  0.00%
 91	     12	  0.00%
 92	     22	  0.00%
 93	     19	  0.00%
 94	     21	  0.00%
 95	     27	  0.00%
 96	     23	  0.00%
 97	     32	  0.00%
 98	     28	  0.00%
 99	     38	  0.00%
100	     30	  0.00%
101	     36	  0.00%
102	     40	  0.00%
103	     35	  0.00%
104	     48	  0.00%
105	     40	  0.00%
106	     59	  0.00%
107	     35	  0.00%
108	     53	  0.00%
109	     43	  0.00%
110	     61	  0.00%
111	     49	  0.00%
112	     59	  0.00%
113	     71	  0.00%
114	     60	  0.00%
115	     62	  0.00%
116	     73	  0.00%
117	     62	  0.00%
118	     78	  0.00%
119	     66	  0.00%
120	     84	  0.00%
121	     86	  0.00%
122	     90	  0.00%
123	    114	  0.00%
124	     85	  0.00%
125	    108	  0.00%
126	    105	  0.00%
127	     91	  0.00%
128	    124	  0.00%
129	    124	  0.00%
130	    112	  0.00%
131	    145	  0.00%
132	    144	  0.00%
133	    165	  0.00%
134	    141	  0.00%
135	    146	  0.00%
136	    159	  0.00%
137	    172	  0.00%
138	    171	  0.00%
139	    193	  0.00%
140	    211	  0.00%
141	   5969	  0.07%
142	   6288	  0.08%
143	   6302	  0.08%
144	   6748	  0.08%
145	   6741	  0.08%
146	   6900	  0.08%
147	   7080	  0.09%
148	   7125	  0.09%
149	   7255	  0.09%
150	8240425	 99.22%


criterion=sequence-density
sequence-density=1.21
sequence-density-rank=1
fanout-score=2.09
fanout-score-rank=38
prefix-density=1.18
prefix-fanout=2.1
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=52.95
fanout-score-rank=1
prefix-density=0.10
prefix-fanout=5.3
sequence=ATCCTCATCAATAAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGGAATGTTGATCCAA


criterion=sequence-density
sequence-density=1.88
sequence-density-rank=1
fanout-score=1.99
fanout-score-rank=39
prefix-density=1.88
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=64.98
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=6.1
sequence=GAGCCAGGAAATTGCGTAAGACTTAAAACCTTGTTCCCAGAGGTTCAAATCCTCTCCCTAATAGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCTAACATTGTTGGTCCATACGGCATTTTACAACCATTTGCAGACGCCATAAAATTATTTATAAAAGAACCAATACGCCCTTTAACAACCTCTATATCCTTATTT
SRR32233443 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 04:17:17
                             Started mapping on |	Apr 17 04:17:17
                                    Finished on |	Apr 17 04:19:55
       Mapping speed, Million of reads per hour |	315.41

                          Number of input reads |	13843037
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11150612
                        Uniquely mapped reads % |	80.55%
                          Average mapped length |	298.87
                       Number of splices: Total |	9029789
            Number of splices: Annotated (sjdb) |	8962271
                       Number of splices: GT/AG |	8945920
                       Number of splices: GC/AG |	68127
                       Number of splices: AT/AC |	7115
               Number of splices: Non-canonical |	8627
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.67
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.34
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2545408
             % of reads mapped to multiple loci |	18.39%
        Number of reads mapped to too many loci |	7632
             % of reads mapped to too many loci |	0.06%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.99%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	147017	147017	147017
N_multimapping	2545408	2545408	2545408
N_noFeature	477934	10447426	748270
N_ambiguous	623338	48997	173935
UnstrandedReadsAssigned:10049340 PositiveStrandReadsAssigned:654189 NegativeStrandReadsAssigned:10228407
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233443 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233443-trimmed-pair1.fastq
                             SRR32233443-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,843,037 reads, 12,327,787 reads pseudoaligned
[quant] estimated average fragment length: 272.468
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,003 rounds

 109283 SRR32233443.ke.tsv
  52636 SRR32233443.se.tsv
 161919 total
==> SRR32233443.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	6.25	0	0
ENSMUST00000196221.1	9	8	0	0
ENSMUST00000179664.1	11	5.5	0	0
ENSMUST00000178537.1	12	6.5	0	0
ENSMUST00000178862.1	14	8.5	0	0
ENSMUST00000179520.1	11	5.5	0	0
ENSMUST00000179883.1	16	6.25	0	0
ENSMUST00000195858.1	10	9	0	0
ENSMUST00000179932.1	12	6.5	0	0

==> SRR32233443.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233443 completed mapping pipeline successfully
