Starting /dee2/code/volunteer_pipeline.sh SRR32233442
    current disk space = 837894872
    free memory = 195734740 
SRR32233442 SRAfilesize
b132967938f127a42050138375c4a6b3  SRR32233442.sra
SRR32233442.sra file validated
SRR32233442 is paired end
SRR32233442 is conventional basespace
SRR32233442 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.6165	37.0	37.0	37.0	37.0	37.0
2	36.5395	37.0	37.0	37.0	37.0	37.0
3	36.60225	37.0	37.0	37.0	37.0	37.0
4	36.679	37.0	37.0	37.0	37.0	37.0
5	36.6735	37.0	37.0	37.0	37.0	37.0
6	36.6925	37.0	37.0	37.0	37.0	37.0
7	36.5745	37.0	37.0	37.0	37.0	37.0
8	36.603	37.0	37.0	37.0	37.0	37.0
9	36.6515	37.0	37.0	37.0	37.0	37.0
10-14	36.63955	37.0	37.0	37.0	37.0	37.0
15-19	36.646800000000006	37.0	37.0	37.0	37.0	37.0
20-24	36.6225	37.0	37.0	37.0	37.0	37.0
25-29	36.645399999999995	37.0	37.0	37.0	37.0	37.0
30-34	36.577299999999994	37.0	37.0	37.0	37.0	37.0
35-39	36.563399999999994	37.0	37.0	37.0	37.0	37.0
40-44	36.538799999999995	37.0	37.0	37.0	37.0	37.0
45-49	36.53060000000001	37.0	37.0	37.0	37.0	37.0
50-54	36.4965	37.0	37.0	37.0	37.0	37.0
55-59	36.4694	37.0	37.0	37.0	37.0	37.0
60-64	36.4683	37.0	37.0	37.0	37.0	37.0
65-69	36.3779	37.0	37.0	37.0	37.0	37.0
70-74	36.3381	37.0	37.0	37.0	37.0	37.0
75-79	36.3417	37.0	37.0	37.0	37.0	37.0
80-84	36.244299999999996	37.0	37.0	37.0	37.0	37.0
85-89	36.250099999999996	37.0	37.0	37.0	37.0	37.0
90-94	36.1322	37.0	37.0	37.0	37.0	37.0
95-99	36.1566	37.0	37.0	37.0	37.0	37.0
100-104	36.101099999999995	37.0	37.0	37.0	37.0	37.0
105-109	35.9298	37.0	37.0	37.0	37.0	37.0
110-114	35.876	37.0	37.0	37.0	37.0	37.0
115-119	35.8753	37.0	37.0	37.0	37.0	37.0
120-124	35.762350000000005	37.0	37.0	37.0	37.0	37.0
125-129	35.672900000000006	37.0	37.0	37.0	37.0	37.0
130-134	35.455	37.0	37.0	37.0	34.6	37.0
135-139	35.4392	37.0	37.0	37.0	37.0	37.0
140-144	35.335300000000004	37.0	37.0	37.0	29.8	37.0
145-149	35.1211	37.0	37.0	37.0	25.0	37.0
150	34.966	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	1.0
27	4.0
28	12.0
29	11.0
30	22.0
31	28.0
32	44.0
33	65.0
34	117.0
35	450.0
36	3128.0
37	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.075	9.675	12.6	36.65
2	21.796796796796798	10.16016016016016	34.109109109109106	33.933933933933936
3	23.655913978494624	11.977994498624655	28.107026756689173	36.25906476619154
4	27.375	18.224999999999998	24.875	29.525000000000002
5	27.825	22.35	26.775	23.05
6	26.900000000000002	27.375	28.249999999999996	17.474999999999998
7	17.9	23.5	40.875	17.724999999999998
8	17.424999999999997	25.224999999999998	36.15	21.2
9	17.575	25.825	36.075	20.525
10-14	20.146007300365017	28.171408570428518	29.27646382319116	22.4061203060153
15-19	21.815	26.57	29.409999999999997	22.205
20-24	20.935000000000002	27.16	29.310000000000002	22.595000000000002
25-29	20.26	26.445	29.215000000000003	24.08
30-34	20.04	27.87	26.150000000000002	25.94
35-39	22.15	26.995	27.839999999999996	23.015
40-44	22.09	26.784999999999997	27.605	23.52
45-49	21.565	25.195	28.985	24.255
50-54	21.27	27.245	28.384999999999998	23.1
55-59	20.025000000000002	27.36	30.130000000000003	22.485
60-64	21.975	25.72	28.96	23.345
65-69	21.11	27.134999999999998	27.785	23.97
70-74	20.375	27.279999999999998	28.849999999999998	23.494999999999997
75-79	22.235	26.795	27.92	23.05
80-84	21.04	27.41	27.73	23.82
85-89	20.424999999999997	26.450000000000003	29.095	24.03
90-94	22.45	24.83	29.160000000000004	23.56
95-99	20.599999999999998	26.99	28.999999999999996	23.41
100-104	21.085	25.650000000000002	29.78	23.485
105-109	20.445	24.845	29.24	25.47
110-114	23.055	26.105	27.57	23.27
115-119	21.240000000000002	25.900000000000002	30.165	22.695
120-124	23.350837709427356	23.670917729432357	28.077019254813703	24.901225306326584
125-129	21.060000000000002	25.515	31.135	22.29
130-134	21.38	27.425	28.375	22.82
135-139	22.065	26.77	27.834999999999997	23.330000000000002
140-144	21.115000000000002	26.76	27.73	24.395
145-149	21.685	26.46	27.67	24.185000000000002
150	20.775	30.049999999999997	26.775	22.400000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5
22	1.5
23	0.5
24	0.5
25	0.5
26	3.5
27	9.5
28	16.5
29	23.5
30	30.5
31	34.0
32	28.5
33	34.0
34	52.5
35	62.5
36	79.5
37	88.0
38	117.0
39	200.5
40	262.5
41	265.0
42	227.0
43	228.0
44	228.0
45	164.5
46	150.0
47	196.5
48	218.5
49	184.0
50	148.0
51	144.0
52	129.0
53	132.0
54	112.0
55	58.5
56	55.0
57	47.0
58	25.5
59	26.5
60	31.0
61	25.5
62	12.0
63	5.5
64	6.0
65	5.5
66	4.5
67	7.0
68	18.5
69	21.0
70	12.5
71	13.5
72	10.5
73	6.0
74	9.5
75	7.5
76	6.5
77	6.5
78	3.0
79	1.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1
3	0.025
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.005
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.025
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.367663344407525	25.45
2	19.878183831672203	17.95
3	10.631229235880399	14.399999999999999
4	4.540420819490587	8.200000000000001
5	2.768549280177187	6.25
6	1.716500553709856	4.65
7	1.1074197120708749	3.5000000000000004
8	0.3875968992248062	1.4000000000000001
9	0.7751937984496124	3.15
>10	1.7718715393133997	13.575000000000001
>50	0.05537098560354374	1.4749999999999999
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	59	1.4749999999999999	No Hit
GCTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCTGGTCTTCATGG	39	0.975	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	39	0.975	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	28	0.7000000000000001	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	27	0.675	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	26	0.65	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	23	0.575	No Hit
CTTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGT	23	0.575	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	20	0.5	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	19	0.475	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	19	0.475	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	19	0.475	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	17	0.42500000000000004	No Hit
CTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAAC	17	0.42500000000000004	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	17	0.42500000000000004	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	16	0.4	No Hit
CTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAACTGTTT	16	0.4	No Hit
CCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATCAT	14	0.35000000000000003	No Hit
GTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCTA	13	0.325	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	12	0.3	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	12	0.3	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	12	0.3	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	11	0.27499999999999997	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	11	0.27499999999999997	No Hit
GCAGTATCTAATTTTATACAATTTAACAAGCAAACACTTTTATTCTCCCA	11	0.27499999999999997	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	11	0.27499999999999997	No Hit
CCCGGGGATAATGCCGGACATATTCTCCAACATCGTGAGCAGCAACAGCT	11	0.27499999999999997	No Hit
GCTTCCTCAATCTGAGATCCCAGTTCTTCTTCATCAGCTCCAATTGCAAC	10	0.25	No Hit
GCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGTG	10	0.25	No Hit
CTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAA	10	0.25	No Hit
GCTTCCTTTAGTGCAAGTTCAACCTCTAACTTACATCTCATGTCTGACAA	10	0.25	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	10	0.25	No Hit
CTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCACTGA	10	0.25	No Hit
CCATGGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTT	9	0.22499999999999998	No Hit
CCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACACT	9	0.22499999999999998	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	9	0.22499999999999998	No Hit
GCCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTC	9	0.22499999999999998	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	9	0.22499999999999998	No Hit
CCTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCACTG	9	0.22499999999999998	No Hit
GGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATG	9	0.22499999999999998	No Hit
CCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAGCA	9	0.22499999999999998	No Hit
CCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATCATT	9	0.22499999999999998	No Hit
GGCATTTAAGACAGACTGTCAGGTGAACAACAAAGACCAACCCATGAGAT	9	0.22499999999999998	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	9	0.22499999999999998	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	9	0.22499999999999998	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	9	0.22499999999999998	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	9	0.22499999999999998	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	8	0.2	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	8	0.2	No Hit
CCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAA	8	0.2	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	8	0.2	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	8	0.2	No Hit
CCCGGAGCATCAGCTGCAGACAGCCAGCAACACACTGCACATACTGAGAA	8	0.2	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	8	0.2	No Hit
GTACAGGTGCAGAGCGCTGGGGGGCGCCAGGATCCCCGGCATCGCAGGGC	7	0.17500000000000002	No Hit
CTCCAAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCAGA	7	0.17500000000000002	No Hit
GCCTTCCATCAGTTCTTTCCCCCAAGCAGCTAACTTGGCGATAATTCTTG	7	0.17500000000000002	No Hit
TTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTG	7	0.17500000000000002	No Hit
GTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACAT	7	0.17500000000000002	No Hit
GTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCC	7	0.17500000000000002	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	7	0.17500000000000002	No Hit
CTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCA	7	0.17500000000000002	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	7	0.17500000000000002	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	7	0.17500000000000002	No Hit
GTGGACTGTAGCAATTCTAGGGTCATGGGAATATTCTTAAGCTCTTTCAG	7	0.17500000000000002	No Hit
CCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTC	7	0.17500000000000002	No Hit
CAAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCAGATTT	7	0.17500000000000002	No Hit
CGCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTAC	7	0.17500000000000002	No Hit
AGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGT	7	0.17500000000000002	No Hit
CTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCT	7	0.17500000000000002	No Hit
TCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTT	7	0.17500000000000002	No Hit
GCGACGAAGGTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTG	7	0.17500000000000002	No Hit
GGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCGT	7	0.17500000000000002	No Hit
GTCCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAG	7	0.17500000000000002	No Hit
TCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCC	6	0.15	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	6	0.15	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	6	0.15	No Hit
CTCGGAACTTCTTTTCATTTCAAACCTTTGAATAAATTCACAATCTTCAG	6	0.15	No Hit
GTGGGCCACCACGCCCGGCAGACCAATCCTTTTAACATGGACATATTGTT	6	0.15	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	6	0.15	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	6	0.15	No Hit
CTCCCTCCACCCGCTTCATGCGCTTCACCTGCTTGCGCCGCCGCGGCCGG	6	0.15	No Hit
CGGGGATAATGCCGGACATATTCTCCAACATCGTGAGCAGCAACAGCTAA	6	0.15	No Hit
CTCCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACA	6	0.15	No Hit
CTGGAACTTGAAATCCTAAATGCTGGATCATAGCCTTAACTTACTGTGCA	6	0.15	No Hit
GGATCATCTGATACCTCCAGAAGTTTTGTCAAGATTTTCAAGAGTTCATA	6	0.15	No Hit
GGCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	6	0.15	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	6	0.15	No Hit
CTCTGACTGTAGTTGTTTACCAAGATATTCCCAGTTGTGCACCATGAGCT	6	0.15	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	6	0.15	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	6	0.15	No Hit
CCCAGTTGTGCACCATGAGCTTCTGCACGGCCAGAAGAGCATTGTAGCGA	6	0.15	No Hit
CTCCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATC	6	0.15	No Hit
GTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCT	6	0.15	No Hit
TTCAGTTTGAAGCATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCAA	6	0.15	No Hit
AGGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATC	6	0.15	No Hit
GCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCAT	6	0.15	No Hit
GTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTT	6	0.15	No Hit
TGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCG	6	0.15	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	6	0.15	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	6	0.15	No Hit
GTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAG	6	0.15	No Hit
GTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATT	6	0.15	No Hit
GCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTG	6	0.15	No Hit
GTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAG	6	0.15	No Hit
GGAGAGAAAATGCCGTGAGGTTGAATGAGAAGAACTATGAACTCTTGAAA	5	0.125	No Hit
GCACGAACTTCAGCAGCCTTGGCCGCAATGATGTTAGTTGGGACAGCAGC	5	0.125	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	5	0.125	No Hit
CTTTGCATTGAATCATAGCCAGAGCATACTCTTGGCGAGTTTCTCTTTCC	5	0.125	No Hit
CACAAGTGAACAAGTCAGTCTGGGTCCCACCAGTCTTGGCCATCTGATGC	5	0.125	No Hit
GCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTGGCTGGATTTA	5	0.125	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	5	0.125	No Hit
GGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGTC	5	0.125	No Hit
AGCAATTCCAGCAAAGCCCATCTCTGAGATGGGGGTGTCGATGATCCTCT	5	0.125	No Hit
GTCAGAACTATAATTTTTAGGTGTGCTAAATAAAAAGTGTGTGCCACTAT	5	0.125	No Hit
ATGAGATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCT	5	0.125	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	5	0.125	No Hit
TCCTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTAC	5	0.125	No Hit
CCTCAACTGTTCAAGTGGCAGACAAAATAAATTACCATAAATTATATGCC	5	0.125	No Hit
GTGAGCAGCAACAGCTAAGACTTGTGGATCATCTGATACCTCCAGAAGTT	5	0.125	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	5	0.125	No Hit
AGTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCT	5	0.125	No Hit
TCCGTGCGGTCCACCTCCCCTAGGAGCTCAGTGGGCTGGTTGGATTCCGT	5	0.125	No Hit
CTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACT	5	0.125	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	5	0.125	No Hit
CTCCTGAATTAAGTGTTCTTTGTCTTGCTCGTGGTTCTTGACCAGTCTCT	5	0.125	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	5	0.125	No Hit
CACTGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATAC	5	0.125	No Hit
GAGAAATAAAAGAAATGATCACTGTGGTTAGTCACAGATGCAAGAGATCC	5	0.125	No Hit
CTCCATCTGCTTCTACCCAGGCAAACCGGTACTCATTGACCCGGAGCATC	5	0.125	No Hit
ATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTCATT	5	0.125	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	5	0.125	No Hit
TCCTACTTCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCT	5	0.125	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
GCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTAT	5	0.125	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	5	0.125	No Hit
AGGGTCATGGGAATATTCTTAAGCTCTTTCAGCAAATCCAATGCGCCAGC	5	0.125	No Hit
CCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTC	5	0.125	No Hit
CTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTCCA	5	0.125	No Hit
CCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAG	5	0.125	No Hit
CCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTCA	5	0.125	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	5	0.125	No Hit
GTGCACCATGAGCTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCT	5	0.125	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	5	0.125	No Hit
CTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTC	5	0.125	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	5	0.125	No Hit
GTCTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCA	5	0.125	No Hit
CTCCCGAGTGCTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCT	5	0.125	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	5	0.125	No Hit
CCTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTT	5	0.125	No Hit
CCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGC	5	0.125	No Hit
CTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTT	5	0.125	No Hit
CTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATTT	5	0.125	No Hit
GCCGCAATGATGTTAGTTGGGACAGCAGCATCCACAGCACCTCGAATGTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCAGGA	10	0.0069863307	143.91249	1
>>END_MODULE
SRR32233442 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233442_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3865	37.0	37.0	37.0	37.0	37.0
2	36.572	37.0	37.0	37.0	37.0	37.0
3	36.61	37.0	37.0	37.0	37.0	37.0
4	36.564	37.0	37.0	37.0	37.0	37.0
5	36.583	37.0	37.0	37.0	37.0	37.0
6	36.5885	37.0	37.0	37.0	37.0	37.0
7	36.625	37.0	37.0	37.0	37.0	37.0
8	36.67	37.0	37.0	37.0	37.0	37.0
9	36.6125	37.0	37.0	37.0	37.0	37.0
10-14	36.599900000000005	37.0	37.0	37.0	37.0	37.0
15-19	36.6066	37.0	37.0	37.0	37.0	37.0
20-24	36.5911	37.0	37.0	37.0	37.0	37.0
25-29	36.5389	37.0	37.0	37.0	37.0	37.0
30-34	36.50169999999999	37.0	37.0	37.0	37.0	37.0
35-39	36.49400000000001	37.0	37.0	37.0	37.0	37.0
40-44	36.4365	37.0	37.0	37.0	37.0	37.0
45-49	36.373	37.0	37.0	37.0	37.0	37.0
50-54	36.3888	37.0	37.0	37.0	37.0	37.0
55-59	36.2655	37.0	37.0	37.0	37.0	37.0
60-64	36.2524	37.0	37.0	37.0	37.0	37.0
65-69	36.1829	37.0	37.0	37.0	37.0	37.0
70-74	36.1402	37.0	37.0	37.0	37.0	37.0
75-79	36.127199999999995	37.0	37.0	37.0	37.0	37.0
80-84	36.0022	37.0	37.0	37.0	37.0	37.0
85-89	35.9576	37.0	37.0	37.0	37.0	37.0
90-94	35.8384	37.0	37.0	37.0	37.0	37.0
95-99	35.6498	37.0	37.0	37.0	37.0	37.0
100-104	35.56609999999999	37.0	37.0	37.0	37.0	37.0
105-109	35.5976	37.0	37.0	37.0	37.0	37.0
110-114	35.45155	37.0	37.0	37.0	37.0	37.0
115-119	35.3042	37.0	37.0	37.0	29.8	37.0
120-124	35.1107	37.0	37.0	37.0	25.0	37.0
125-129	35.0009	37.0	37.0	37.0	25.0	37.0
130-134	34.7881	37.0	37.0	37.0	25.0	37.0
135-139	34.5596	37.0	37.0	37.0	25.0	37.0
140-144	34.4991	37.0	37.0	37.0	25.0	37.0
145-149	34.37520000000001	37.0	37.0	37.0	25.0	37.0
150	34.169	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	0.0
23	0.0
24	1.0
25	1.0
26	5.0
27	4.0
28	8.0
29	14.0
30	27.0
31	20.0
32	36.0
33	95.0
34	264.0
35	1051.0
36	2434.0
37	38.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.667333666833414	22.861430715357677	12.8064032016008	29.664832416208103
2	22.45	33.825	28.125	15.6
3	23.125	32.7	22.475	21.7
4	26.150000000000002	35.949999999999996	19.75	18.15
5	23.825	38.224999999999994	20.65	17.299999999999997
6	21.45	38.05	23.400000000000002	17.1
7	21.575	22.525000000000002	35.949999999999996	19.950000000000003
8	23.825	23.799999999999997	27.55	24.825
9	24.55	23.825	28.199999999999996	23.425
10-14	25.924999999999997	27.88	24.075	22.12
15-19	24.41	28.725	25.395	21.47
20-24	24.42	29.515	24.93	21.135
25-29	24.72	28.050000000000004	25.074999999999996	22.155
30-34	24.23	27.265	26.855	21.65
35-39	24.15	28.044999999999998	26.405	21.4
40-44	24.26	28.17	25.155	22.415
45-49	23.435	28.849999999999998	25.580000000000002	22.134999999999998
50-54	23.255	28.92	25.074999999999996	22.75
55-59	23.1	28.199999999999996	26.855	21.845
60-64	23.76	28.82	25.619999999999997	21.8
65-69	24.46	27.88	26.13	21.529999999999998
70-74	23.325000000000003	28.444999999999997	26.21	22.02
75-79	23.985	28.110000000000003	26.245	21.66
80-84	23.74	28.84	25.595000000000002	21.825
85-89	23.875	27.894999999999996	26.21	22.02
90-94	23.435	29.360000000000003	24.88	22.325
95-99	23.47	28.15	27.37	21.01
100-104	23.26	28.59	26.255	21.895
105-109	24.89	28.02	25.259999999999998	21.83
110-114	23.2161608080404	28.641432071603578	26.94634731736587	21.19605980299015
115-119	24.14	27.805000000000003	25.77	22.285
120-124	23.74	28.155	26.565	21.54
125-129	24.095	28.125	26.205000000000002	21.575
130-134	24.195	28.244999999999997	25.855	21.705
135-139	23.615	29.455	25.11	21.82
140-144	23.95	29.299999999999997	26.279999999999998	20.47
145-149	23.845	28.52	26.045	21.59
150	22.875	30.575000000000003	25.974999999999998	20.575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.0
21	0.0
22	0.0
23	0.0
24	2.0
25	5.0
26	7.5
27	14.0
28	16.5
29	18.5
30	18.5
31	18.5
32	28.5
33	43.0
34	52.0
35	61.0
36	92.0
37	101.0
38	148.5
39	223.5
40	283.5
41	298.0
42	238.0
43	203.5
44	192.0
45	154.0
46	137.0
47	160.0
48	178.0
49	153.0
50	105.0
51	90.0
52	116.0
53	132.0
54	99.0
55	64.5
56	43.5
57	46.0
58	44.5
59	29.5
60	26.5
61	18.0
62	13.0
63	15.0
64	9.0
65	17.0
66	27.5
67	31.5
68	29.0
69	19.5
70	30.0
71	44.5
72	40.0
73	23.0
74	12.5
75	8.0
76	4.0
77	4.0
78	4.0
79	2.0
80	0.5
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.005
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.55
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.5359477124183	30.275000000000002
2	23.622782446311856	25.3
3	10.084033613445378	16.2
4	4.295051353874883	9.2
5	2.0541549953314657	5.5
6	1.353874883286648	4.35
7	0.8870214752567693	3.325
8	0.4668534080298786	2.0
9	0.18674136321195145	0.8999999999999999
>10	0.5135387488328664	2.9499999999999997
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	12	0.3	No Hit
GAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAG	12	0.3	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	11	0.27499999999999997	No Hit
GAAATTTTGGAGAGAAAATGCCGTGAGGTTGAATGAGAAGAACTATGAAC	11	0.27499999999999997	No Hit
GTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGC	11	0.27499999999999997	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	11	0.27499999999999997	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	10	0.25	No Hit
CAGAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGGAACAATCTCTTC	10	0.25	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	10	0.25	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	10	0.25	No Hit
GATGATTTCTGCTGAAGATTGTGAATTTATTCAAAGGTTTGAAATGAAAA	10	0.25	No Hit
GTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCA	9	0.22499999999999998	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	9	0.22499999999999998	No Hit
TTGGAATGGAGCCCTGTACACAAGTCTGAGAAATTTTGGAGAGAAAATGC	9	0.22499999999999998	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	9	0.22499999999999998	No Hit
CTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACTCA	8	0.2	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	8	0.2	No Hit
GAAGAACTATGAACTCTTGAAAATCTTGACAAAACTTCTGGAGGTATCAG	8	0.2	No Hit
CAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTTGCAGCATTCCG	8	0.2	No Hit
CAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTA	8	0.2	No Hit
GAGAATTTGGAGCAGCAGAAGTATGATGATGAAGATATCAGTGAAGACAT	8	0.2	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	8	0.2	No Hit
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	8	0.2	No Hit
CAGACATCCTTCAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTT	8	0.2	No Hit
GTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTATG	8	0.2	No Hit
CGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGG	7	0.17500000000000002	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	7	0.17500000000000002	No Hit
GAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTC	7	0.17500000000000002	No Hit
GGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAA	7	0.17500000000000002	No Hit
GAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTT	7	0.17500000000000002	No Hit
GTAACAAGTGTGGTTTTCAACTTCAATACCAAATGATTTTTTCAATATGG	7	0.17500000000000002	No Hit
CAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGA	7	0.17500000000000002	No Hit
ATTCAATGCAAAGTACTGAAACAGTTGGAGAATTTGGAGCAGCAGAAGTA	7	0.17500000000000002	No Hit
TTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTG	7	0.17500000000000002	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	7	0.17500000000000002	No Hit
CATCCTTCAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTTGCAG	7	0.17500000000000002	No Hit
GATTTTTTCAATATGGCTCCTGGCATTCAGTCCTCAAATGTGTGAGCACC	7	0.17500000000000002	No Hit
GAAGATATCAGTGAAGACATCAAATTTCTTTTGGAAAAACTTGGTGAGAG	7	0.17500000000000002	No Hit
ATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAG	7	0.17500000000000002	No Hit
ACGGAATCCAACCAGCCCACTGAGCTCCTAGGGGAGGTGGACCGCACGGA	7	0.17500000000000002	No Hit
AGATGATCCACAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATG	7	0.17500000000000002	No Hit
GCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAG	7	0.17500000000000002	No Hit
GGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCA	7	0.17500000000000002	No Hit
AGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGG	7	0.17500000000000002	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	6	0.15	No Hit
ATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACT	6	0.15	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	6	0.15	No Hit
AGCTCATCCTTGCCTACTTAGTAGTTAGAAGGCAGCCTGGGCTGTTGTGC	6	0.15	No Hit
TGGCAGTCTTATCTTCAGGGGCAGATGATTTCTGCTGAAGATTGTGAATT	6	0.15	No Hit
TCAGAACTTAAATCTGGAAGATTGGAATGGAGCCCTGTACACAAGTCTGA	6	0.15	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	6	0.15	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	6	0.15	No Hit
GTTGAATCGCCAGGATCCCTTTACTGTTCATATGGCAGCAAGAATTATCG	6	0.15	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	6	0.15	No Hit
CATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA	6	0.15	No Hit
CGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACA	6	0.15	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	6	0.15	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	6	0.15	No Hit
ATTTGGAGCAGCAGAAGTATGATGATGAAGATATCAGTGAAGACATCAAA	6	0.15	No Hit
ATCTGATGACGCACATCTCCAAAGAACAGACAGTGCAGTACATACTAACC	6	0.15	No Hit
GAAAAGAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAG	6	0.15	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	6	0.15	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	6	0.15	No Hit
AAAGAAGTTCCGAGGACAAGCAAGAGATGCTTCAAACTGAAGGCAGCCAG	6	0.15	No Hit
ATGGAAAACAATATGTCCATGTTAAAAGGATTGGTCTGCCGGGCGTGGTG	6	0.15	No Hit
CTCAAATGTGTGAGCACCTTCGTCGCTATAATATCATTCCTGTTCTATCA	6	0.15	No Hit
GGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACAT	6	0.15	No Hit
GCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCC	6	0.15	No Hit
GTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTG	6	0.15	No Hit
TACTATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTTCGTGG	6	0.15	No Hit
GGAAGATTGGAATGGAGCCCTGTACACAAGTCTGAGAAATTTTGGAGAGA	6	0.15	No Hit
CTTAAATTACTATTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAAC	6	0.15	No Hit
GAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGGAACAATCTCTTCAA	6	0.15	No Hit
GGATGAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAG	5	0.125	No Hit
AGCAGATGGAGTGAACTGTATAATGGGCGTTTTGAGTAACAAGTGTGGTT	5	0.125	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	5	0.125	No Hit
AGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAGAAAGTT	5	0.125	No Hit
GAGAAAATGCCGTGAGGTTGAATGAGAAGAACTATGAACTCTTGAAAATC	5	0.125	No Hit
CTCGTAGTGCTGATGAACCAATGACAACATTTGTTGTATGTAATGAATGT	5	0.125	No Hit
CACCACACAGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTC	5	0.125	No Hit
GAAAAGAAGTTCCGAGGACAAGCAAGAGATGCTTCAAACTGAAGGCAGCC	5	0.125	No Hit
GTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAG	5	0.125	No Hit
CAATAACAGTAGTGATCTGGTTCTGTTCTTTCTTAAAATTAGACTGTAAA	5	0.125	No Hit
CAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGG	5	0.125	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	5	0.125	No Hit
ATTTATTCAAAGGTTTGAAATGAAAAGAAGTTCCGAGGACAAGCAAGAGA	5	0.125	No Hit
AGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACA	5	0.125	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	5	0.125	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	5	0.125	No Hit
CAACCAGCCCACTGAGCTCCTAGGGGAGGTGGACCGCACGGAATTCGAAC	5	0.125	No Hit
CAAAAAGTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACC	5	0.125	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	5	0.125	No Hit
GCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGT	5	0.125	No Hit
CCACACAGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCA	5	0.125	No Hit
CTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACC	5	0.125	No Hit
GAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAG	5	0.125	No Hit
GGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCT	5	0.125	No Hit
GGTGCATGTGGTTCATCACCAACTGCTTTCCACCAAGCTGCTCAATAACC	5	0.125	No Hit
GGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGA	5	0.125	No Hit
CAGAACTTAAATCTGGAAGATTGGAATGGAGCCCTGTACACAAGTCTGAG	5	0.125	No Hit
GGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCC	5	0.125	No Hit
GCTTCCTCTGAGTGAGGCTCGCAACGTCTCCCACGGAGTCGCCTTCGTTC	5	0.125	No Hit
GAGCTCAAAGAGATGAGGAAAAACCTGACCAAAGAAGCCATCAGGGAGCA	5	0.125	No Hit
CAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTG	5	0.125	No Hit
GTTCAGAACTTAAATCTGGAAGATTGGAATGGAGCCCTGTACACAAGTCT	5	0.125	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	5	0.125	No Hit
GCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAA	5	0.125	No Hit
CAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCA	5	0.125	No Hit
TTTCAATTGGATAAAAACTCAGCTGAGTTCACAGAAACTTCGTGGCAGCG	5	0.125	No Hit
AAGCAAGAGATGCTTCAAACTGAAGGCAGCCAGTGTGCTAAAACATTTAT	5	0.125	No Hit
CACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTA	5	0.125	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	5	0.125	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	5	0.125	No Hit
GAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGGCAACTCGTTT	5	0.125	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	5	0.125	No Hit
TATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAG	5	0.125	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
Read 1653173 spots for SRR32233442.sra
Written 1653173 spots for SRR32233442.sra
SRR ids: ['SRR32233442.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_6idsl3nm
SRR32233442.sra spots: 13225384
blocks: [[1, 1653173], [1653174, 3306346], [3306347, 4959519], [4959520, 6612692], [6612693, 8265865], [8265866, 9919038], [9919039, 11572211], [11572212, 13225384]]
SRR32233442 file size 4447036
SRR32233442 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233442 SRR32233442_1.fastq SRR32233442_2.fastq
Input file:	SRR32233442_1.fastq
Paired file:	SRR32233442_2.fastq
trimmed:	SRR32233442-trimmed-pair1.fastq, SRR32233442-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:58:05 2026 >> started

Fri Apr 17 04:58:24 2026 >> done (19.493s)
13225384 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
      25 ( 0.00%) empty read pairs filtered out after trimming by size control
13225359 (100.00%) read pairs available; of these:
  238180 ( 1.80%) trimmed read pairs available after processing
12987179 (98.20%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 30	       1	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       1	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       1	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       1	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	       0	  0.00%
 67	       2	  0.00%
 68	       0	  0.00%
 69	       2	  0.00%
 70	       2	  0.00%
 71	       4	  0.00%
 72	       1	  0.00%
 73	       2	  0.00%
 74	       6	  0.00%
 75	       2	  0.00%
 76	       5	  0.00%
 77	       9	  0.00%
 78	       6	  0.00%
 79	       8	  0.00%
 80	       4	  0.00%
 81	       4	  0.00%
 82	       9	  0.00%
 83	      15	  0.00%
 84	       9	  0.00%
 85	      16	  0.00%
 86	      16	  0.00%
 87	       7	  0.00%
 88	      16	  0.00%
 89	      18	  0.00%
 90	      19	  0.00%
 91	      15	  0.00%
 92	      31	  0.00%
 93	      29	  0.00%
 94	      29	  0.00%
 95	      36	  0.00%
 96	      30	  0.00%
 97	      40	  0.00%
 98	      62	  0.00%
 99	      42	  0.00%
100	      56	  0.00%
101	      52	  0.00%
102	      70	  0.00%
103	      70	  0.00%
104	      83	  0.00%
105	      89	  0.00%
106	      70	  0.00%
107	      67	  0.00%
108	      93	  0.00%
109	     108	  0.00%
110	      96	  0.00%
111	     111	  0.00%
112	     103	  0.00%
113	     118	  0.00%
114	     108	  0.00%
115	     130	  0.00%
116	     120	  0.00%
117	     155	  0.00%
118	     145	  0.00%
119	     180	  0.00%
120	     168	  0.00%
121	     201	  0.00%
122	     183	  0.00%
123	     235	  0.00%
124	     213	  0.00%
125	     200	  0.00%
126	     226	  0.00%
127	     266	  0.00%
128	     278	  0.00%
129	     274	  0.00%
130	     294	  0.00%
131	     317	  0.00%
132	     359	  0.00%
133	     361	  0.00%
134	     383	  0.00%
135	     426	  0.00%
136	     410	  0.00%
137	     407	  0.00%
138	     498	  0.00%
139	     513	  0.00%
140	     500	  0.00%
141	   22499	  0.17%
142	   23113	  0.17%
143	   23362	  0.18%
144	   24438	  0.18%
145	   25928	  0.20%
146	   26565	  0.20%
147	   26982	  0.20%
148	   27679	  0.21%
149	   28377	  0.21%
150	12987179	 98.20%
13225359 reads passed initial QC


criterion=sequence-density
sequence-density=4.15
sequence-density-rank=1
fanout-score=2.23
fanout-score-rank=35
prefix-density=4.51
prefix-fanout=2.0
sequence=TGTTGATCCAATAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=59.06
fanout-score-rank=1
prefix-density=0.30
prefix-fanout=1.1
sequence=TATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTATC


criterion=sequence-density
sequence-density=0.74
sequence-density-rank=1
fanout-score=3.62
fanout-score-rank=28
prefix-density=2.68
prefix-fanout=1.0
sequence=TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=53.01
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=3.4
sequence=CTTCCTGCTGCCGGGCCGGTTTGAGGCTACTCTGAAGCGACCCAGCGGTTCTGCCCGACGCGCCCGCTCGAGACACCATGGTGGCGTACTGGCGACAGGCTGGACTCAGCTACATCCGGTTTTCCCAGATCTGTGCAAAAGCAGTGAGGGATGCCCTGAAGACCGAGTTCAAAGCGAACGCTGAGAAGACTTCGGGCAGCAGCATAAAAATTGTGAAAGTCTCGAAGAAGGAGTAGCTGAATCTGAAGCCTGAAGTGCTGAGTCTTGAAGGTGAAGCATGTGGGCCCCTGTTCTGGCAGATGGAAATCAACCTCACCT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAA -y TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGA -o SRR32233442 SRR32233442_1.fastq SRR32233442_2.fastq
Input file:	SRR32233442_1.fastq
Paired file:	SRR32233442_2.fastq
trimmed:	SRR32233442-trimmed-pair1.fastq, SRR32233442-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAA
-- paired 3' end adapter sequence (-y):	TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:59:06 2026 >> started

Fri Apr 17 04:59:12 2026 >> done (5.994s)
4408453 read pairs processed; of these:
     39 ( 0.00%) short read pairs filtered out after trimming by size control
    121 ( 0.00%) empty read pairs filtered out after trimming by size control
4408293 (100.00%) read pairs available; of these:
      6 ( 0.00%) trimmed read pairs available after processing
4408287 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	      1	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      1	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      2	  0.00%
 68	      0	  0.00%
 69	      1	  0.00%
 70	      1	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      4	  0.00%
 75	      0	  0.00%
 76	      2	  0.00%
 77	      4	  0.00%
 78	      4	  0.00%
 79	      4	  0.00%
 80	      0	  0.00%
 81	      1	  0.00%
 82	      3	  0.00%
 83	      7	  0.00%
 84	      4	  0.00%
 85	      7	  0.00%
 86	      8	  0.00%
 87	      3	  0.00%
 88	      7	  0.00%
 89	      3	  0.00%
 90	      4	  0.00%
 91	      3	  0.00%
 92	      8	  0.00%
 93	     13	  0.00%
 94	      6	  0.00%
 95	     10	  0.00%
 96	      7	  0.00%
 97	     21	  0.00%
 98	     18	  0.00%
 99	     17	  0.00%
100	     25	  0.00%
101	     19	  0.00%
102	     22	  0.00%
103	     28	  0.00%
104	     16	  0.00%
105	     33	  0.00%
106	     31	  0.00%
107	     30	  0.00%
108	     31	  0.00%
109	     32	  0.00%
110	     33	  0.00%
111	     37	  0.00%
112	     25	  0.00%
113	     41	  0.00%
114	     25	  0.00%
115	     37	  0.00%
116	     39	  0.00%
117	     48	  0.00%
118	     48	  0.00%
119	     68	  0.00%
120	     59	  0.00%
121	     74	  0.00%
122	     60	  0.00%
123	     79	  0.00%
124	     71	  0.00%
125	     58	  0.00%
126	     69	  0.00%
127	     94	  0.00%
128	     96	  0.00%
129	     77	  0.00%
130	    107	  0.00%
131	     97	  0.00%
132	    105	  0.00%
133	    126	  0.00%
134	    125	  0.00%
135	    146	  0.00%
136	    120	  0.00%
137	    125	  0.00%
138	    168	  0.00%
139	    173	  0.00%
140	    157	  0.00%
141	   7610	  0.17%
142	   7816	  0.18%
143	   8096	  0.18%
144	   8260	  0.19%
145	   8481	  0.19%
146	   9015	  0.20%
147	   8816	  0.20%
148	   9133	  0.21%
149	   9499	  0.22%
150	4328539	 98.19%


criterion=sequence-density
sequence-density=1.35
sequence-density-rank=1
fanout-score=2.13
fanout-score-rank=38
prefix-density=1.37
prefix-fanout=2.1
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=27
fanout-score=70.77
fanout-score-rank=1
prefix-density=0.34
prefix-fanout=8.9
sequence=CTCCTTCTCCTAGCATCATCAATAGTAGAAGCAGGAGCAGGAACAGGATGAACAGTCTACCCACCTCTAGCCGGAAATCTAGCCCATGCAGGAGCATCAGTAGACCTAACAATTTTCTCCCTTCATTTAGCTGGAGTGTCATCTATTTTAGGTGCAATTAATTTTATTACCACTATTATCAACATGAAACCCCCAGCCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACAGCCGTACTGCTCCTATTATCACTACCAGTGCTAGCCGCAGGCATTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCCGCTGGAGGAGGGGACCCAATTCTCTACCAGCATCTGTTCTGATTCT


criterion=sequence-density
sequence-density=0.33
sequence-density-rank=1
fanout-score=3.34
fanout-score-rank=40
prefix-density=0.57
prefix-fanout=1.9
sequence=CATAACCCTAGTATTATTTTTCCCAGACATACTAGGAGACCCAGACAACTACATACCAGCTAATCCACTAAACACCCCACCCCATATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAATCCCCAATAAACTAGGAGGTGTCCTAGCCTTAATCTTATCTATCCTAATTTTAGCCCTAATACCTTTCCTTCATACCTCAAAGCAACGAAGCCTAATATTCCGCCCAATCACACAAATTTTGTACTGAATCCTAGTAGCCAACCTACTTATCTTAACCTGAATTGGGGGCCAACCAGTAGAACACCCATTTATTAT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=36.13
fanout-score-rank=1
prefix-density=0.56
prefix-fanout=1.1
sequence=AATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTAATCCTTATCATAATTGCTCTCCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTTCTGCCAATAGAACTTCCAATCCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATCAAACCGACCAGGGTTATTCTATGGCCAATGCTCTGAA
SRR32233442 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 05:01:18
                             Started mapping on |	Apr 17 05:01:18
                                    Finished on |	Apr 17 05:04:44
       Mapping speed, Million of reads per hour |	231.12

                          Number of input reads |	13225199
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11396330
                        Uniquely mapped reads % |	86.17%
                          Average mapped length |	299.07
                       Number of splices: Total |	11144828
            Number of splices: Annotated (sjdb) |	11062837
                       Number of splices: GT/AG |	11041996
                       Number of splices: GC/AG |	82699
                       Number of splices: AT/AC |	9250
               Number of splices: Non-canonical |	10883
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.74
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.30
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1729768
             % of reads mapped to multiple loci |	13.08%
        Number of reads mapped to too many loci |	13832
             % of reads mapped to too many loci |	0.10%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.62%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	99101	99101	99101
N_multimapping	1729768	1729768	1729768
N_noFeature	453017	10376245	1058994
N_ambiguous	595357	23279	178041
UnstrandedReadsAssigned:10347956 PositiveStrandReadsAssigned:996806 NegativeStrandReadsAssigned:10159295
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233442 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233442-trimmed-pair1.fastq
                             SRR32233442-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,225,199 reads, 11,697,794 reads pseudoaligned
[quant] estimated average fragment length: 230.367
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,123 rounds

 109283 SRR32233442.ke.tsv
  52636 SRR32233442.se.tsv
 161919 total
==> SRR32233442.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9.5	0	0
ENSMUST00000196221.1	9	5	0	0
ENSMUST00000179664.1	11	7	0	0
ENSMUST00000178537.1	12	8	0	0
ENSMUST00000178862.1	14	10	0	0
ENSMUST00000179520.1	11	7	0	0
ENSMUST00000179883.1	16	9.5	0	0
ENSMUST00000195858.1	10	6	0	0
ENSMUST00000179932.1	12	8	0	0

==> SRR32233442.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233442 completed mapping pipeline successfully
