Starting /dee2/code/volunteer_pipeline.sh SRR32233446
    current disk space = 825958112
    free memory = 199297708 
SRR32233446 SRAfilesize
d94799afccc56951e52cc691ad6903d7  SRR32233446.sra
SRR32233446.sra file validated
SRR32233446 is paired end
SRR32233446 is conventional basespace
SRR32233446 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.47025	37.0	37.0	37.0	37.0	37.0
2	36.2955	37.0	37.0	37.0	37.0	37.0
3	36.5525	37.0	37.0	37.0	37.0	37.0
4	36.4185	37.0	37.0	37.0	37.0	37.0
5	36.51	37.0	37.0	37.0	37.0	37.0
6	36.4625	37.0	37.0	37.0	37.0	37.0
7	36.4065	37.0	37.0	37.0	37.0	37.0
8	36.494	37.0	37.0	37.0	37.0	37.0
9	36.545	37.0	37.0	37.0	37.0	37.0
10-14	36.5169	37.0	37.0	37.0	37.0	37.0
15-19	36.473949999999995	37.0	37.0	37.0	37.0	37.0
20-24	36.5033	37.0	37.0	37.0	37.0	37.0
25-29	36.4564	37.0	37.0	37.0	37.0	37.0
30-34	36.406000000000006	37.0	37.0	37.0	37.0	37.0
35-39	36.420399999999994	37.0	37.0	37.0	37.0	37.0
40-44	36.3454	37.0	37.0	37.0	37.0	37.0
45-49	36.26195	37.0	37.0	37.0	37.0	37.0
50-54	36.26825	37.0	37.0	37.0	37.0	37.0
55-59	36.2136	37.0	37.0	37.0	37.0	37.0
60-64	36.22115000000001	37.0	37.0	37.0	37.0	37.0
65-69	36.190099999999994	37.0	37.0	37.0	37.0	37.0
70-74	36.16025	37.0	37.0	37.0	37.0	37.0
75-79	36.09779999999999	37.0	37.0	37.0	37.0	37.0
80-84	36.0825	37.0	37.0	37.0	37.0	37.0
85-89	36.13735	37.0	37.0	37.0	37.0	37.0
90-94	35.93390000000001	37.0	37.0	37.0	37.0	37.0
95-99	35.9387	37.0	37.0	37.0	37.0	37.0
100-104	35.8925	37.0	37.0	37.0	37.0	37.0
105-109	35.841300000000004	37.0	37.0	37.0	37.0	37.0
110-114	35.779399999999995	37.0	37.0	37.0	37.0	37.0
115-119	35.8502	37.0	37.0	37.0	37.0	37.0
120-124	35.6533	37.0	37.0	37.0	37.0	37.0
125-129	35.6262	37.0	37.0	37.0	37.0	37.0
130-134	35.483399999999996	37.0	37.0	37.0	37.0	37.0
135-139	35.52570000000001	37.0	37.0	37.0	37.0	37.0
140-144	35.3912	37.0	37.0	37.0	32.2	37.0
145-149	35.0536	37.0	37.0	37.0	25.0	37.0
150	35.195	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	6.0
26	2.0
27	14.0
28	18.0
29	24.0
30	41.0
31	51.0
32	49.0
33	109.0
34	142.0
35	386.0
36	2988.0
37	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.26056514128532	9.877469367341837	13.753438359589898	34.10852713178294
2	19.7	9.925	38.975	31.4
3	23.325000000000003	10.9	31.025000000000002	34.75
4	26.8	16.375	28.199999999999996	28.625
5	28.875	20.175	27.650000000000002	23.3
6	24.3	28.599999999999998	30.75	16.35
7	15.875	23.325000000000003	44.175	16.625
8	16.125	27.425	38.6	17.849999999999998
9	17.150000000000002	29.799999999999997	34.575	18.475
10-14	19.455	31.35	30.085	19.11
15-19	21.236061803090152	28.666433321666084	30.861543077153858	19.235961798089903
20-24	19.345000000000002	32.195	29.255	19.205
25-29	18.87	30.035	29.375	21.72
30-34	19.42	28.749999999999996	27.74	24.09
35-39	20.047004700470048	29.192919291929194	29.74797479747975	21.012101210121013
40-44	19.869999999999997	31.209999999999997	27.560000000000002	21.36
45-49	19.46597329866493	28.07140357017851	31.096554827741386	21.36606830341517
50-54	19.50792618892834	29.38440766114917	29.429414412161826	21.678251737760664
55-59	17.72177217721772	31.293129312931296	30.34803480348035	20.637063706370636
60-64	20.41102055102755	29.17145857292865	28.626431321566077	21.791089554477725
65-69	20.665	28.025	28.945	22.365
70-74	20.15100755037752	30.281514075703786	28.401420071003553	21.166058302915143
75-79	19.955000000000002	29.825000000000003	28.845	21.375
80-84	19.634999999999998	28.660000000000004	30.53	21.175
85-89	18.475923796189807	28.671433571678584	30.676533826691333	22.176108805440272
90-94	22.082666132906326	28.012409927942354	29.258406725380304	20.646517213771016
95-99	19.115	30.4	31.135	19.35
100-104	19.855	29.57	29.659999999999997	20.915
105-109	17.96	26.884999999999998	33.46	21.695
110-114	21.005	29.12	27.744999999999997	22.13
115-119	18.92	28.04	32.39	20.65
120-124	21.09	25.75	31.495	21.665
125-129	19.61	28.310000000000002	32.405	19.675
130-134	19.945	28.055000000000003	31.545	20.455000000000002
135-139	19.189999999999998	29.445	28.64	22.725
140-144	18.55	29.080000000000002	31.775	20.595
145-149	19.96	28.660000000000004	28.93	22.45
150	21.2	29.775000000000002	27.375	21.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.5
20	1.5
21	3.0
22	4.0
23	7.0
24	11.5
25	21.5
26	47.5
27	75.0
28	117.5
29	141.0
30	123.0
31	102.0
32	75.5
33	54.5
34	78.5
35	95.0
36	120.5
37	138.5
38	124.5
39	163.5
40	193.0
41	173.0
42	160.5
43	225.5
44	227.0
45	137.0
46	139.5
47	180.0
48	205.5
49	169.0
50	100.5
51	92.5
52	77.0
53	59.5
54	50.5
55	38.0
56	51.0
57	46.5
58	20.0
59	13.5
60	18.0
61	13.5
62	4.5
63	8.0
64	8.0
65	3.0
66	3.0
67	9.0
68	15.5
69	10.0
70	5.5
71	9.0
72	8.5
73	4.5
74	2.0
75	3.5
76	5.0
77	2.0
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.005
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.01
40-44	0.0
45-49	0.005
50-54	0.015
55-59	0.01
60-64	0.005
65-69	0.0
70-74	0.005
75-79	0.0
80-84	0.0
85-89	0.005
90-94	0.08
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.45664739884393	24.85
2	18.728323699421964	16.2
3	9.132947976878611	11.85
4	5.144508670520231	8.9
5	2.832369942196532	6.125
6	1.7919075144508672	4.65
7	0.9248554913294799	2.8000000000000003
8	0.8670520231213872	3.0
9	0.5780346820809248	2.25
>10	2.485549132947977	17.775
>50	0.05780346820809249	1.6
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	64	1.6	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	38	0.95	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	33	0.8250000000000001	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	33	0.8250000000000001	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	33	0.8250000000000001	No Hit
GCTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAA	29	0.7250000000000001	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	26	0.65	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	25	0.625	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	25	0.625	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	25	0.625	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	24	0.6	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	20	0.5	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	19	0.475	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	17	0.42500000000000004	No Hit
GTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTA	17	0.42500000000000004	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	16	0.4	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	16	0.4	No Hit
GTTGGTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATT	15	0.375	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	15	0.375	No Hit
GTTCTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTAT	15	0.375	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	14	0.35000000000000003	No Hit
CTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAAC	14	0.35000000000000003	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	14	0.35000000000000003	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	13	0.325	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	13	0.325	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	12	0.3	No Hit
TCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	12	0.3	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	12	0.3	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	11	0.27499999999999997	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	11	0.27499999999999997	No Hit
CGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATT	11	0.27499999999999997	No Hit
GGTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTT	11	0.27499999999999997	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	11	0.27499999999999997	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	11	0.27499999999999997	No Hit
CACCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACA	10	0.25	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	10	0.25	No Hit
CCTGAACACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATA	10	0.25	No Hit
CCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATC	10	0.25	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	10	0.25	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	10	0.25	No Hit
GTTGGACTAAGGTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTG	10	0.25	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	10	0.25	No Hit
GCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGT	10	0.25	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	10	0.25	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	9	0.22499999999999998	No Hit
CTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAA	9	0.22499999999999998	No Hit
ATGAGATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCT	9	0.22499999999999998	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	9	0.22499999999999998	No Hit
CTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATGAA	9	0.22499999999999998	No Hit
GGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTC	9	0.22499999999999998	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	9	0.22499999999999998	No Hit
GTCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGG	9	0.22499999999999998	No Hit
GGTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGAT	9	0.22499999999999998	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	9	0.22499999999999998	No Hit
GCCTTCCATCAGTTCTTTCCCCCAAGCAGCTAACTTGGCGATAATTCTTG	8	0.2	No Hit
GGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTAAAA	8	0.2	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	8	0.2	No Hit
GCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAAT	8	0.2	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	8	0.2	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	8	0.2	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	8	0.2	No Hit
CTCTTTGACAGATTCTTGAAGGATGTCTGATAGAACAGGAATGATATTAT	8	0.2	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	8	0.2	No Hit
GAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACG	8	0.2	No Hit
GTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATT	8	0.2	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	8	0.2	No Hit
GGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAA	8	0.2	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	8	0.2	No Hit
CCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACT	8	0.2	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	7	0.17500000000000002	No Hit
GTCTGATAGAACAGGAATGATATTATAGCGACGAAGGTGCTCACACATTT	7	0.17500000000000002	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	7	0.17500000000000002	No Hit
CTTGCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTT	7	0.17500000000000002	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	7	0.17500000000000002	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	7	0.17500000000000002	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	7	0.17500000000000002	No Hit
CCTCCCTCCACCCGCTTCATGCGCTTCACCTGCTTGCGCCGCCGCGGCCG	7	0.17500000000000002	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	7	0.17500000000000002	No Hit
GTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCACTTACATCTTACC	7	0.17500000000000002	No Hit
CCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGAC	7	0.17500000000000002	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	7	0.17500000000000002	No Hit
AGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGT	7	0.17500000000000002	No Hit
CATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTC	7	0.17500000000000002	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	7	0.17500000000000002	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	7	0.17500000000000002	No Hit
TCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTT	6	0.15	No Hit
GTGGGCCACCACGCCCGGCAGACCAATCCTTTTAACATGGACATATTGTT	6	0.15	No Hit
ATCCAATTGAAATAGTAATTTAAGTCACTGCCTTCCATCAGTTCTTTCCC	6	0.15	No Hit
CTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCT	6	0.15	No Hit
GTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCC	6	0.15	No Hit
CCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACACT	6	0.15	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	6	0.15	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	6	0.15	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	6	0.15	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	6	0.15	No Hit
ATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGG	6	0.15	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	6	0.15	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	6	0.15	No Hit
GTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCTA	6	0.15	No Hit
GACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCT	6	0.15	No Hit
GTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCT	6	0.15	No Hit
ACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	6	0.15	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	6	0.15	No Hit
CTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTCCA	6	0.15	No Hit
CCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAG	6	0.15	No Hit
CGCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTAC	6	0.15	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	6	0.15	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	6	0.15	No Hit
CTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCT	6	0.15	No Hit
GTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATC	6	0.15	No Hit
CTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTC	6	0.15	No Hit
GGGATCCTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCT	6	0.15	No Hit
GCTCCAAATTCTCCAACTGTTTCAGTACTTTGCATTGAATCATAGCCAGA	6	0.15	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	6	0.15	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	6	0.15	No Hit
CCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGA	6	0.15	No Hit
GATTGTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAG	5	0.125	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	5	0.125	No Hit
TATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAG	5	0.125	No Hit
ATTGGAATACACACTACATTTGGTAACTCTAAAAATTTATATTATGCCTT	5	0.125	No Hit
GTCTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACA	5	0.125	No Hit
GCCTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAA	5	0.125	No Hit
ACTGCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACA	5	0.125	No Hit
CTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAA	5	0.125	No Hit
GTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCA	5	0.125	No Hit
GTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAGGACA	5	0.125	No Hit
ATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCTGTATA	5	0.125	No Hit
GGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTG	5	0.125	No Hit
TGGTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTT	5	0.125	No Hit
ATTGAAGTTGAAAACCACACTTGTTACTCAAAACGCCCATTATACAGTTC	5	0.125	No Hit
CTGAAGATAAGACTGCCAGTTCACTTTGTTGGCACGAACTTCAGCAGCCT	5	0.125	No Hit
GCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTG	5	0.125	No Hit
GTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCTAACCT	5	0.125	No Hit
GTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGC	5	0.125	No Hit
GTTGGGGTGGTCCTGCATATGCTGCACGCGCAGCCGCTCGGCCTCTTCCA	5	0.125	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	5	0.125	No Hit
AAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATTTCTAAAT	5	0.125	No Hit
GCTTTTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGA	5	0.125	No Hit
CTCGTGTAGCCCCTCAACTGTTCAAGTGGCAGACAAAATAAATTACCATA	5	0.125	No Hit
CCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTCC	5	0.125	No Hit
CTCCCTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCAT	5	0.125	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	5	0.125	No Hit
GCTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCAC	5	0.125	No Hit
CCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCC	5	0.125	No Hit
CCTCTGCTAACCTTGTATGCACCGTCATACTGGGCAACTTCTTCCCCAAG	5	0.125	No Hit
CCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTAT	5	0.125	No Hit
GTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACTGG	5	0.125	No Hit
GTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTT	5	0.125	No Hit
GCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATT	5	0.125	No Hit
GTCTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTTGATGA	5	0.125	No Hit
ACCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATT	5	0.125	No Hit
TGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCG	5	0.125	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	5	0.125	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	5	0.125	No Hit
GGCCTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAA	5	0.125	No Hit
GGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATT	5	0.125	No Hit
TCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCT	5	0.125	No Hit
GTTTGTGTTTAGGGTTTCTTAGATGCATTTTCTTACCATTGTAAGAAAAA	5	0.125	No Hit
GGTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCA	5	0.125	No Hit
CCCCTCCAAAAGGCATCTTCCCCTCACAGCCTCCACGCCAAACAAGGAGC	5	0.125	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	5	0.125	No Hit
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	5	0.125	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	5	0.125	No Hit
GTTTGTTATCTTATTTTGCTGAATTTGAGACAAAGAATCTAGTTGCTTCA	5	0.125	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32233446 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233446_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0415	37.0	37.0	37.0	37.0	37.0
2	36.3195	37.0	37.0	37.0	37.0	37.0
3	36.355	37.0	37.0	37.0	37.0	37.0
4	36.2935	37.0	37.0	37.0	37.0	37.0
5	36.2635	37.0	37.0	37.0	37.0	37.0
6	36.2625	37.0	37.0	37.0	37.0	37.0
7	36.255	37.0	37.0	37.0	37.0	37.0
8	36.365	37.0	37.0	37.0	37.0	37.0
9	36.3445	37.0	37.0	37.0	37.0	37.0
10-14	36.3504	37.0	37.0	37.0	37.0	37.0
15-19	36.3042	37.0	37.0	37.0	37.0	37.0
20-24	36.25619999999999	37.0	37.0	37.0	37.0	37.0
25-29	36.1877	37.0	37.0	37.0	37.0	37.0
30-34	36.1988	37.0	37.0	37.0	37.0	37.0
35-39	36.2085	37.0	37.0	37.0	37.0	37.0
40-44	36.1747	37.0	37.0	37.0	37.0	37.0
45-49	36.086400000000005	37.0	37.0	37.0	37.0	37.0
50-54	36.0601	37.0	37.0	37.0	37.0	37.0
55-59	35.98910000000001	37.0	37.0	37.0	37.0	37.0
60-64	35.976099999999995	37.0	37.0	37.0	37.0	37.0
65-69	35.9782	37.0	37.0	37.0	37.0	37.0
70-74	35.90839999999999	37.0	37.0	37.0	37.0	37.0
75-79	35.8343	37.0	37.0	37.0	37.0	37.0
80-84	35.7102	37.0	37.0	37.0	37.0	37.0
85-89	35.636199999999995	37.0	37.0	37.0	37.0	37.0
90-94	35.653499999999994	37.0	37.0	37.0	37.0	37.0
95-99	35.6214	37.0	37.0	37.0	37.0	37.0
100-104	35.562400000000004	37.0	37.0	37.0	37.0	37.0
105-109	35.482299999999995	37.0	37.0	37.0	37.0	37.0
110-114	35.3986	37.0	37.0	37.0	32.2	37.0
115-119	35.3153	37.0	37.0	37.0	32.2	37.0
120-124	35.2114	37.0	37.0	37.0	25.0	37.0
125-129	34.959900000000005	37.0	37.0	37.0	25.0	37.0
130-134	34.8711	37.0	37.0	37.0	25.0	37.0
135-139	34.8445	37.0	37.0	37.0	25.0	37.0
140-144	34.6028	37.0	37.0	37.0	25.0	37.0
145-149	34.5834	37.0	37.0	37.0	25.0	37.0
150	34.322	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.0
24	3.0
25	3.0
26	2.0
27	12.0
28	15.0
29	29.0
30	45.0
31	38.0
32	65.0
33	128.0
34	305.0
35	961.0
36	2324.0
37	66.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.1	23.575	14.174999999999999	29.15
2	19.8	35.5	31.05	13.65
3	20.95	32.6	27.500000000000004	18.95
4	22.375	35.699999999999996	23.75	18.175
5	22.775000000000002	36.075	25.25	15.9
6	20.200000000000003	40.300000000000004	22.875	16.625
7	21.55	20.75	37.85	19.85
8	20.974999999999998	24.275	30.725	24.025
9	24.474999999999998	24.75	28.65	22.125
10-14	24.075	28.185	25.435000000000002	22.305
15-19	23.3	29.14	26.91	20.65
20-24	22.625	30.19	27.13	20.055
25-29	22.67	29.425	26.99	20.915
30-34	22.7	27.275	29.404999999999998	20.62
35-39	22.735	28.95	28.189999999999998	20.125
40-44	21.94	29.970000000000002	28.185	19.905
45-49	23.02	29.970000000000002	26.314999999999998	20.695
50-54	20.495	30.509999999999998	27.74	21.255
55-59	20.405	30.645	28.33	20.62
60-64	21.41	29.830000000000002	28.410000000000004	20.349999999999998
65-69	21.62	30.45	28.02	19.91
70-74	22.025	29.145	28.87	19.96
75-79	21.740000000000002	28.99	29.07	20.200000000000003
80-84	21.990000000000002	29.875	27.589999999999996	20.544999999999998
85-89	22.355	29.715000000000003	28.15	19.78
90-94	21.95	30.635	27.694999999999997	19.72
95-99	21.27	29.475	30.154999999999998	19.1
100-104	22.095000000000002	29.555	28.189999999999998	20.16
105-109	23.005	29.080000000000002	27.805000000000003	20.11
110-114	21.09	30.245	28.28	20.385
115-119	21.41	29.5	28.49	20.599999999999998
120-124	21.37	29.685	29.020000000000003	19.925
125-129	21.475	30.03	28.645	19.85
130-134	21.75	28.875	28.17	21.205
135-139	20.745	31.535000000000004	27.689999999999998	20.03
140-144	21.765	30.654999999999998	28.335	19.245
145-149	21.22	30.06	29.12	19.6
150	21.6	30.975	29.075	18.35
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	4.0
20	5.0
21	4.5
22	13.5
23	24.0
24	30.0
25	30.0
26	36.5
27	65.0
28	84.5
29	76.5
30	72.0
31	77.5
32	88.0
33	114.5
34	111.5
35	96.5
36	109.0
37	111.0
38	129.5
39	176.0
40	201.5
41	219.0
42	222.5
43	200.5
44	175.5
45	143.5
46	139.5
47	142.0
48	142.5
49	101.0
50	60.0
51	79.5
52	78.5
53	70.0
54	64.0
55	50.0
56	35.5
57	29.0
58	30.5
59	39.5
60	37.5
61	19.0
62	10.5
63	14.0
64	21.0
65	23.0
66	20.5
67	22.5
68	23.0
69	18.0
70	24.5
71	30.5
72	21.0
73	9.0
74	5.5
75	7.0
76	4.5
77	2.0
78	1.0
79	0.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.275000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.81922525107605	30.225
2	20.803443328550934	21.75
3	9.612625538020087	15.075
4	5.308464849354376	11.1
5	2.8694404591104736	7.5
6	1.4347202295552368	4.5
7	0.8130081300813009	2.9749999999999996
8	0.38259206121472983	1.6
9	0.19129603060736491	0.8999999999999999
>10	0.7651841224294597	4.375
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	14	0.35000000000000003	No Hit
GTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGC	12	0.3	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	12	0.3	No Hit
GGACCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAAC	11	0.27499999999999997	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	11	0.27499999999999997	No Hit
GTTTTATTTTTATCTGGGTAACATATTATTTTGAAATTTTCATCTTAAGT	11	0.27499999999999997	No Hit
CTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACTCA	11	0.27499999999999997	No Hit
CCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAAT	11	0.27499999999999997	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	11	0.27499999999999997	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	11	0.27499999999999997	No Hit
ATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACT	10	0.25	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	10	0.25	No Hit
GAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCAA	10	0.25	No Hit
GATAAATTGCTTTTCTCATTGGTATGTACCTAAACATGTTAAAAGGAAAA	10	0.25	No Hit
CAGAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGGAACAATCTCTTC	10	0.25	No Hit
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	10	0.25	No Hit
GTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCT	9	0.22499999999999998	No Hit
AACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAA	9	0.22499999999999998	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	9	0.22499999999999998	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	9	0.22499999999999998	No Hit
GTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGA	8	0.2	No Hit
TGAATGTGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAA	8	0.2	No Hit
CAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCA	8	0.2	No Hit
CATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCG	8	0.2	No Hit
GTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATA	8	0.2	No Hit
CCTAAACATGTTAAAAGGAAAAGGCATAATATAAATTTTTAGAGTTACCA	8	0.2	No Hit
GTTCAAGATGAGCAATCAGACTTATGTTTTATTTTTATCTGGGTAACATA	8	0.2	No Hit
GCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAG	8	0.2	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	7	0.17500000000000002	No Hit
AAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATC	7	0.17500000000000002	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	7	0.17500000000000002	No Hit
AAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTC	7	0.17500000000000002	No Hit
TTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATATTTTAT	7	0.17500000000000002	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	7	0.17500000000000002	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	7	0.17500000000000002	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	7	0.17500000000000002	No Hit
GTTATAGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGT	7	0.17500000000000002	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	7	0.17500000000000002	No Hit
CAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAG	7	0.17500000000000002	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	7	0.17500000000000002	No Hit
CAGACTTATGTTTTATTTTTATCTGGGTAACATATTATTTTGAAATTTTC	7	0.17500000000000002	No Hit
CAAATATTAAAATAATAATTTGATTTTAAATCATCAGCATGTTCAAAATA	7	0.17500000000000002	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	7	0.17500000000000002	No Hit
AGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCA	7	0.17500000000000002	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	7	0.17500000000000002	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	6	0.15	No Hit
AGCAGATGGAGTGAACTGTATAATGGGCGTTTTGAGTAACAAGTGTGGTT	6	0.15	No Hit
CGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGAT	6	0.15	No Hit
ATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGC	6	0.15	No Hit
CCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCTGGTAA	6	0.15	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	6	0.15	No Hit
GTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACACAGGTGC	6	0.15	No Hit
CAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAACAAACAAAC	6	0.15	No Hit
GTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATTACTTAAGTTT	6	0.15	No Hit
ATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCT	6	0.15	No Hit
TCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATG	6	0.15	No Hit
ACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCC	6	0.15	No Hit
GGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCAGCTTCTCT	6	0.15	No Hit
ATTAAATCGGCCATTCGTGATAATAACCCAGTGGTAATGCTAGAAAATGA	6	0.15	No Hit
CAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGA	6	0.15	No Hit
CTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAGCAGAAA	6	0.15	No Hit
GAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAG	6	0.15	No Hit
AATTTTTAGAGTTACCAAATGTAGTGTGTATTCCAATAGTATGTGGCCAG	6	0.15	No Hit
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	6	0.15	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	6	0.15	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	6	0.15	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	6	0.15	No Hit
GTTGTAATGCATATAGTAAAGGCCACTTTTATAAATTAAGTGCTTCTGCC	6	0.15	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	6	0.15	No Hit
ATTATTTTGAAATTTTCATCTTAAGTAAAGGAGCTTTTGATAATCAGTCT	6	0.15	No Hit
ATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAG	6	0.15	No Hit
GAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGGCAACTCGTTT	6	0.15	No Hit
GTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCGCA	6	0.15	No Hit
GTGAAGATGTGTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTT	6	0.15	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	6	0.15	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	5	0.125	No Hit
CTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCGCG	5	0.125	No Hit
AAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGT	5	0.125	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	5	0.125	No Hit
CACAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAA	5	0.125	No Hit
CTCGTAGTGCTGATGAACCAATGACAACATTTGTTGTATGTAATGAATGT	5	0.125	No Hit
TAAGAATCAGATGGTATTACATTATCCCTAATAATGGGGATAATGCTTTT	5	0.125	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	5	0.125	No Hit
AGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAAT	5	0.125	No Hit
GTTGAATCGCCAGGATCCCTTTACTGTTCATATGGCAGCAAGAATTATCG	5	0.125	No Hit
AGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCT	5	0.125	No Hit
GTTTACATTTTCTGTACATGTTATGCTTAAAACCAAATACTGTTCAAGAT	5	0.125	No Hit
AAAAACTCAGCTGAGTTCACAGAAACTTCGTGGCAGCGGTGTTGCTGTTG	5	0.125	No Hit
TGCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATC	5	0.125	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	5	0.125	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	5	0.125	No Hit
CGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGG	5	0.125	No Hit
GAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAA	5	0.125	No Hit
TTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACT	5	0.125	No Hit
ATCTGATGACGCACATCTCCAAAGAACAGACAGTGCAGTACATACTAACC	5	0.125	No Hit
GCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAG	5	0.125	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	5	0.125	No Hit
GCTGAAGTTCGTGCCAACAAAGTGAACTGGCAGTCTTATCTTCAGGGGCA	5	0.125	No Hit
CACAGAGAAACCCTGTCTCGGAAACAAACAAACAAACAAACCGAAACACA	5	0.125	No Hit
AAAGTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACCAGG	5	0.125	No Hit
CAATGACAACATTTGTTGTATGTAATGAATGTGGAAATCGGTGGAAGTTC	5	0.125	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	5	0.125	No Hit
GAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAA	5	0.125	No Hit
GGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATC	5	0.125	No Hit
AAAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTT	5	0.125	No Hit
CACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTC	5	0.125	No Hit
CAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATT	5	0.125	No Hit
CGGAAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAA	5	0.125	No Hit
CACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAA	5	0.125	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	5	0.125	No Hit
CAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATC	5	0.125	No Hit
TTGTTGTTCACCTGACAGTCTGTCTTAAATGCCTTCTGTGGTCTCAGATC	5	0.125	No Hit
CAGGAACAATCTCTTCAAGTGATAGTTCTCAGTATGTGCAGTGTGTTGCT	5	0.125	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	5	0.125	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	5	0.125	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	5	0.125	No Hit
AATCAGTCTCCTGTACAAATATTAAAATAATAATTTGATTTTAAATCATC	5	0.125	No Hit
CTCGTTTCCTTGCAAGTGAAATTTGTAAACAACATACATCTCATGGGTTG	5	0.125	No Hit
AAACCGTTGCACAAGCTTTGCACTCTTCTATGCTCTGCTTTGTAGACCGT	5	0.125	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	5	0.125	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	5	0.125	No Hit
CGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCCAG	5	0.125	No Hit
GAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAA	5	0.125	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	5	0.125	No Hit
GTATTTTTCTGTTTGTATTCACATAGATGATACTATAATAGCTAAGAATC	5	0.125	No Hit
TCTGAGTGAGGCTCGCAACGTCTCCCACGGAGTCGCCTTCGTTCTGCTCT	5	0.125	No Hit
GCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCC	5	0.125	No Hit
GGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAAT	5	0.125	No Hit
CCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGA	5	0.125	No Hit
GTGAACTGTATAATGGGCGTTTTGAGTAACAAGTGTGGTTTTCAACTTCA	5	0.125	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	5	0.125	No Hit
GAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCC	5	0.125	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	5	0.125	No Hit
AATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAAC	5	0.125	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2026-04-17T04:31:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:31:54 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:31:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:31:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:31:56 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:31:58 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:05 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:11 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
2026-04-17T04:32:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:14 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:15 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:17 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
2026-04-17T04:32:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:32:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1828298 spots for SRR32233446.sra
Written 1828298 spots for SRR32233446.sra
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
Read 1828294 spots for SRR32233446.sra
Written 1828294 spots for SRR32233446.sra
SRR ids: ['SRR32233446.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_nt3cnm_s
SRR32233446.sra spots: 14626356
blocks: [[1, 1828294], [1828295, 3656588], [3656589, 5484882], [5484883, 7313176], [7313177, 9141470], [9141471, 10969764], [10969765, 12798058], [12798059, 14626356]]
SRR32233446 file size 4920412
SRR32233446 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233446 SRR32233446_1.fastq SRR32233446_2.fastq
Input file:	SRR32233446_1.fastq
Paired file:	SRR32233446_2.fastq
trimmed:	SRR32233446-trimmed-pair1.fastq, SRR32233446-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:33:03 2026 >> started

Fri Apr 17 04:33:18 2026 >> done (15.518s)
14626356 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       7 ( 0.00%) empty read pairs filtered out after trimming by size control
14626349 (100.00%) read pairs available; of these:
  160142 ( 1.09%) trimmed read pairs available after processing
14466207 (98.91%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 57	       1	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       1	  0.00%
 67	       0	  0.00%
 68	       2	  0.00%
 69	       4	  0.00%
 70	       3	  0.00%
 71	       8	  0.00%
 72	       4	  0.00%
 73	       6	  0.00%
 74	       4	  0.00%
 75	       4	  0.00%
 76	      11	  0.00%
 77	       7	  0.00%
 78	       6	  0.00%
 79	       7	  0.00%
 80	      11	  0.00%
 81	      16	  0.00%
 82	      14	  0.00%
 83	      12	  0.00%
 84	      15	  0.00%
 85	      15	  0.00%
 86	      16	  0.00%
 87	      23	  0.00%
 88	      24	  0.00%
 89	      34	  0.00%
 90	      26	  0.00%
 91	      37	  0.00%
 92	      49	  0.00%
 93	      50	  0.00%
 94	      38	  0.00%
 95	      44	  0.00%
 96	      53	  0.00%
 97	      40	  0.00%
 98	      53	  0.00%
 99	      68	  0.00%
100	      76	  0.00%
101	      74	  0.00%
102	      75	  0.00%
103	      91	  0.00%
104	      85	  0.00%
105	      96	  0.00%
106	     101	  0.00%
107	     108	  0.00%
108	      90	  0.00%
109	     124	  0.00%
110	     146	  0.00%
111	     132	  0.00%
112	     144	  0.00%
113	     149	  0.00%
114	     139	  0.00%
115	     138	  0.00%
116	     148	  0.00%
117	     183	  0.00%
118	     191	  0.00%
119	     170	  0.00%
120	     179	  0.00%
121	     192	  0.00%
122	     189	  0.00%
123	     215	  0.00%
124	     231	  0.00%
125	     248	  0.00%
126	     233	  0.00%
127	     256	  0.00%
128	     271	  0.00%
129	     255	  0.00%
130	     301	  0.00%
131	     307	  0.00%
132	     333	  0.00%
133	     338	  0.00%
134	     332	  0.00%
135	     358	  0.00%
136	     371	  0.00%
137	     394	  0.00%
138	     408	  0.00%
139	     391	  0.00%
140	     442	  0.00%
141	   15123	  0.10%
142	   15391	  0.11%
143	   15801	  0.11%
144	   15881	  0.11%
145	   16608	  0.11%
146	   17127	  0.12%
147	   18094	  0.12%
148	   18049	  0.12%
149	   18658	  0.13%
150	14466207	 98.91%
14626349 reads passed initial QC


criterion=sequence-density
sequence-density=2.63
sequence-density-rank=1
fanout-score=2.64
fanout-score-rank=33
prefix-density=6.94
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=51.82
fanout-score-rank=1
prefix-density=0.51
prefix-fanout=1.3
sequence=TTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTA


criterion=sequence-density
sequence-density=2.85
sequence-density-rank=1
fanout-score=5.01
fanout-score-rank=24
prefix-density=7.43
prefix-fanout=1.9
sequence=AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=67.77
fanout-score-rank=1
prefix-density=0.32
prefix-fanout=1.1
sequence=TAAAAACTTCAGAGCAGACCTCATCAGTATAATATAATTAGGAGGCCAGTTTTTCCTGGCAAGCGTAAAAGCGAAAGAATTATGGACTAAAACATAGCCCTGTGCTGTATCACGGCCACAGTATATTGTAAACTCTGTCTAATCATGGATTGTGTCACTGTCTCTGTTGAGTGAGGTGATCGTGGGAGTGGCAAGCGTGTGTTGCGACTTGAGCCCGGTTGTGCTGCACACACAGATGAAGCCGTCCTCTGCACACTTCCTTTATCATGTGTTTTCACCGTGCTGCACAC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT -y AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA -o SRR32233446 SRR32233446_1.fastq SRR32233446_2.fastq
Input file:	SRR32233446_1.fastq
Paired file:	SRR32233446_2.fastq
trimmed:	SRR32233446-trimmed-pair1.fastq, SRR32233446-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATA
-- paired 3' end adapter sequence (-y):	AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:35:25 2026 >> started

Fri Apr 17 04:35:41 2026 >> done (16.002s)
4875450 read pairs processed; of these:
     34 ( 0.00%) short read pairs filtered out after trimming by size control
   1115 ( 0.02%) empty read pairs filtered out after trimming by size control
4874301 (99.98%) read pairs available; of these:
      4 ( 0.00%) trimmed read pairs available after processing
4874297 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 68	      2	  0.00%
 69	      2	  0.00%
 70	      1	  0.00%
 71	      2	  0.00%
 72	      1	  0.00%
 73	      1	  0.00%
 74	      3	  0.00%
 75	      1	  0.00%
 76	      3	  0.00%
 77	      2	  0.00%
 78	      0	  0.00%
 79	      3	  0.00%
 80	      5	  0.00%
 81	      7	  0.00%
 82	      7	  0.00%
 83	      6	  0.00%
 84	      4	  0.00%
 85	      6	  0.00%
 86	      8	  0.00%
 87	      6	  0.00%
 88	      8	  0.00%
 89	     15	  0.00%
 90	     10	  0.00%
 91	     14	  0.00%
 92	     17	  0.00%
 93	     19	  0.00%
 94	     10	  0.00%
 95	     14	  0.00%
 96	     18	  0.00%
 97	     12	  0.00%
 98	     12	  0.00%
 99	     24	  0.00%
100	     28	  0.00%
101	     18	  0.00%
102	     29	  0.00%
103	     29	  0.00%
104	     23	  0.00%
105	     33	  0.00%
106	     42	  0.00%
107	     40	  0.00%
108	     37	  0.00%
109	     39	  0.00%
110	     40	  0.00%
111	     28	  0.00%
112	     49	  0.00%
113	     45	  0.00%
114	     50	  0.00%
115	     47	  0.00%
116	     48	  0.00%
117	     77	  0.00%
118	     66	  0.00%
119	     64	  0.00%
120	     55	  0.00%
121	     56	  0.00%
122	     59	  0.00%
123	     77	  0.00%
124	     80	  0.00%
125	     94	  0.00%
126	     78	  0.00%
127	     99	  0.00%
128	     98	  0.00%
129	     72	  0.00%
130	    106	  0.00%
131	     97	  0.00%
132	    117	  0.00%
133	    137	  0.00%
134	     96	  0.00%
135	    114	  0.00%
136	    122	  0.00%
137	    145	  0.00%
138	    140	  0.00%
139	    137	  0.00%
140	    152	  0.00%
141	   5050	  0.10%
142	   4997	  0.10%
143	   5165	  0.11%
144	   5206	  0.11%
145	   5708	  0.12%
146	   5577	  0.11%
147	   5891	  0.12%
148	   5909	  0.12%
149	   6285	  0.13%
150	4821307	 98.91%


criterion=sequence-density
sequence-density=1.16
sequence-density-rank=1
fanout-score=2.08
fanout-score-rank=39
prefix-density=1.17
prefix-fanout=2.1
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=33
fanout-score=41.50
fanout-score-rank=1
prefix-density=1.59
prefix-fanout=1.1
sequence=GGGCAGCCATGCAGTCGATGAAGAACGTGGTGAAGTTGATGTGCCAGCGGTACTCACTGGCAGAGTAGTCGAAGGGGAAGGTGTGGTGGTAGTTGTGGAAGCCCTCGCCCACGGCACCCAGGGAAACCAGGATATTCTCCCGGGATTGAATGTTCTTGTCGTAGGGGCGATATCCATAGAGATGCGCGGCACTGTTCACCAGCCAGGTGGCGTTGAGCACCAGAGTGTATCGCAAGAAGGTGCTAACGAACAGGCTGTTTACAAAAGTCTCGCCCCAGCAGTACCAGGGCACCAGCGTGGGCAGGATGAAGCACATCAGCAGGAGGCCGGGCTTGTAGTACCTCCTCTGGAACATCACCAGCTTCTCGGCTTTCAGGTCAGACATGTCCAGTTTTCCGCCCTTCTCTTTGACAGCCGGGTGTTTGCGCACAAGCAGCCAACCCACGTGAGAGAAGAAGAAGCCACGGCGGGAATTGTGAGGGTCGGCGTGTGTTTCTGAGAACTTGTGGTG


criterion=sequence-density
sequence-density=1.32
sequence-density-rank=1
fanout-score=2.27
fanout-score-rank=39
prefix-density=1.41
prefix-fanout=2.1
sequence=CCTAATCATATT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=36.57
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=3.7
sequence=CAGAGCCAGGAAATTGCGTAAGACTTAAAACCTTGTTCCCAGAGGTTCAAATCCTCTCCCTAATAGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCTAACATTGTTGGTCCATACGGCATTTTACAACCATTTGCAGACGCCATAAAATTATTTATAAAAGAACCAATACGCCCTTTAACAACCTCTATATCCTTATTT
SRR32233446 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 04:36:57
                             Started mapping on |	Apr 17 04:36:57
                                    Finished on |	Apr 17 04:39:23
       Mapping speed, Million of reads per hour |	360.62

                          Number of input reads |	14625200
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	12001233
                        Uniquely mapped reads % |	82.06%
                          Average mapped length |	298.89
                       Number of splices: Total |	10246165
            Number of splices: Annotated (sjdb) |	10172215
                       Number of splices: GT/AG |	10151426
                       Number of splices: GC/AG |	76517
                       Number of splices: AT/AC |	8349
               Number of splices: Non-canonical |	9873
                      Mismatch rate per base, % |	0.20%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.72
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.38
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2466082
             % of reads mapped to multiple loci |	16.86%
        Number of reads mapped to too many loci |	12524
             % of reads mapped to too many loci |	0.09%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.97%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	157885	157885	157885
N_multimapping	2466082	2466082	2466082
N_noFeature	412871	11077519	892724
N_ambiguous	626874	46540	167159
UnstrandedReadsAssigned:10961488 PositiveStrandReadsAssigned:877174 NegativeStrandReadsAssigned:10941350
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233446 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233446-trimmed-pair1.fastq
                             SRR32233446-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 14,625,200 reads, 12,968,492 reads pseudoaligned
[quant] estimated average fragment length: 251.536
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,285 rounds

 109283 SRR32233446.ke.tsv
  52636 SRR32233446.se.tsv
 161919 total
==> SRR32233446.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7	0	0
ENSMUST00000196221.1	9	2	0	0
ENSMUST00000179664.1	11	4	0	0
ENSMUST00000178537.1	12	3	0	0
ENSMUST00000178862.1	14	5	0	0
ENSMUST00000179520.1	11	4	0	0
ENSMUST00000179883.1	16	7	0	0
ENSMUST00000195858.1	10	3	0	0
ENSMUST00000179932.1	12	3	0	0

==> SRR32233446.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233446 completed mapping pipeline successfully
