Starting /dee2/code/volunteer_pipeline.sh SRR32233444
    current disk space = 830159412
    free memory = 191397648 
SRR32233444 SRAfilesize
6089216ab2f6089af2818f1f31f1efd1  SRR32233444.sra
SRR32233444.sra file validated
SRR32233444 is paired end
SRR32233444 is conventional basespace
SRR32233444 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.425	37.0	37.0	37.0	37.0	37.0
2	36.32225	37.0	37.0	37.0	37.0	37.0
3	36.559	37.0	37.0	37.0	37.0	37.0
4	36.527	37.0	37.0	37.0	37.0	37.0
5	36.4735	37.0	37.0	37.0	37.0	37.0
6	36.493	37.0	37.0	37.0	37.0	37.0
7	36.4035	37.0	37.0	37.0	37.0	37.0
8	36.473	37.0	37.0	37.0	37.0	37.0
9	36.5	37.0	37.0	37.0	37.0	37.0
10-14	36.53789999999999	37.0	37.0	37.0	37.0	37.0
15-19	36.4599	37.0	37.0	37.0	37.0	37.0
20-24	36.486000000000004	37.0	37.0	37.0	37.0	37.0
25-29	36.4766	37.0	37.0	37.0	37.0	37.0
30-34	36.4442	37.0	37.0	37.0	37.0	37.0
35-39	36.3683	37.0	37.0	37.0	37.0	37.0
40-44	36.3704	37.0	37.0	37.0	37.0	37.0
45-49	36.37065	37.0	37.0	37.0	37.0	37.0
50-54	36.30985	37.0	37.0	37.0	37.0	37.0
55-59	36.26389999999999	37.0	37.0	37.0	37.0	37.0
60-64	36.210300000000004	37.0	37.0	37.0	37.0	37.0
65-69	36.210899999999995	37.0	37.0	37.0	37.0	37.0
70-74	36.1244	37.0	37.0	37.0	37.0	37.0
75-79	36.1595	37.0	37.0	37.0	37.0	37.0
80-84	36.0837	37.0	37.0	37.0	37.0	37.0
85-89	36.1575	37.0	37.0	37.0	37.0	37.0
90-94	36.01865	37.0	37.0	37.0	37.0	37.0
95-99	36.0034	37.0	37.0	37.0	37.0	37.0
100-104	35.9697	37.0	37.0	37.0	37.0	37.0
105-109	35.827200000000005	37.0	37.0	37.0	37.0	37.0
110-114	35.7505	37.0	37.0	37.0	37.0	37.0
115-119	35.81529999999999	37.0	37.0	37.0	37.0	37.0
120-124	35.785399999999996	37.0	37.0	37.0	37.0	37.0
125-129	35.6846	37.0	37.0	37.0	37.0	37.0
130-134	35.56179999999999	37.0	37.0	37.0	37.0	37.0
135-139	35.5976	37.0	37.0	37.0	37.0	37.0
140-144	35.428999999999995	37.0	37.0	37.0	37.0	37.0
145-149	35.277499999999996	37.0	37.0	37.0	25.0	37.0
150	35.038	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	3.0
25	2.0
26	6.0
27	9.0
28	8.0
29	16.0
30	38.0
31	51.0
32	76.0
33	82.0
34	128.0
35	413.0
36	3014.0
37	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05	10.15	12.625	35.175
2	18.74843554443054	9.887359198998748	36.7459324155194	34.618272841051315
3	21.825	11.875	28.95	37.35
4	25.174999999999997	18.775	27.3	28.749999999999996
5	30.625000000000004	20.225	27.55	21.6
6	24.05	28.675	31.0	16.275000000000002
7	16.275000000000002	22.6	44.7	16.425
8	15.375	26.974999999999998	39.550000000000004	18.099999999999998
9	16.325	29.45	36.625	17.599999999999998
10-14	18.785	32.425	30.225	18.565
15-19	20.57	29.580000000000002	30.904999999999998	18.945
20-24	18.154999999999998	33.0	29.5	19.345000000000002
25-29	18.295	29.854999999999997	31.15	20.7
30-34	19.45	28.88	28.235	23.435
35-39	19.301930193019302	30.033003300330037	30.363036303630363	20.3020302030203
40-44	20.375	29.865000000000002	28.455000000000002	21.305
45-49	19.457918687803172	29.304395659348902	30.359553933089963	20.878131719757963
50-54	19.167875181277193	29.339400910136522	30.084512676901536	21.408211231684753
55-59	17.405	32.1	29.995	20.5
60-64	20.375	30.425	28.22	20.979999999999997
65-69	20.135	28.744999999999997	28.985	22.134999999999998
70-74	19.845	31.045	28.02	21.09
75-79	20.025000000000002	29.775000000000002	29.4	20.8
80-84	20.305	28.555000000000003	30.385	20.755000000000003
85-89	18.360000000000003	28.825	31.025000000000002	21.790000000000003
90-94	22.169410116575776	27.01255816280582	30.0595387001551	20.758493020463302
95-99	19.72	29.985	30.959999999999997	19.335
100-104	18.925	30.445	29.45	21.18
105-109	18.61	27.63	32.82	20.94
110-114	20.375	29.735	28.175	21.715
115-119	19.2	28.48	31.72	20.599999999999998
120-124	21.765	26.085	31.075000000000003	21.075
125-129	19.325	27.96	32.519999999999996	20.195
130-134	20.305	28.67	30.620000000000005	20.405
135-139	19.335	30.37	26.995	23.3
140-144	18.72	28.804999999999996	31.715	20.76
145-149	20.244999999999997	30.29	28.084999999999997	21.38
150	20.625	29.65	28.199999999999996	21.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.5
14	0.5
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	2.5
21	7.5
22	9.5
23	8.0
24	13.0
25	25.0
26	47.5
27	76.0
28	129.0
29	169.5
30	161.0
31	117.0
32	91.0
33	66.0
34	61.0
35	99.5
36	146.5
37	160.5
38	124.0
39	135.5
40	184.5
41	166.5
42	147.0
43	205.0
44	194.5
45	115.0
46	112.0
47	170.0
48	178.0
49	137.5
50	118.5
51	108.0
52	79.5
53	65.0
54	55.5
55	37.5
56	40.5
57	37.5
58	25.5
59	26.0
60	33.0
61	23.5
62	7.5
63	4.0
64	1.5
65	2.5
66	4.0
67	4.5
68	7.5
69	8.5
70	7.0
71	8.5
72	4.5
73	4.5
74	7.0
75	4.5
76	4.0
77	4.5
78	2.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.125
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.01
40-44	0.0
45-49	0.015
50-54	0.015
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.065
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.46576945582213	24.125
2	21.06495026331188	18.0
3	8.835576360444705	11.325000000000001
4	4.212990052662375	7.199999999999999
5	2.1064950263311877	4.5
6	1.4043300175541251	3.5999999999999996
7	1.0532475131655938	3.15
8	0.994733762434172	3.4000000000000004
9	0.9362200117027502	3.5999999999999996
>10	2.8086600351082502	18.45
>50	0.11702750146284377	2.65
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	54	1.35	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	52	1.3	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	39	0.975	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	39	0.975	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	28	0.7000000000000001	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	26	0.65	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	25	0.625	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	23	0.575	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	22	0.5499999999999999	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	19	0.475	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	19	0.475	No Hit
GCTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAA	18	0.44999999999999996	No Hit
GTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTA	18	0.44999999999999996	No Hit
GTTGGTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATT	17	0.42500000000000004	No Hit
GCCAGTCACAAGTATTTCTAAATATGCCAAGATATTACTTCCCCTTTAAG	16	0.4	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	16	0.4	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	16	0.4	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	16	0.4	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	16	0.4	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	15	0.375	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	14	0.35000000000000003	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	14	0.35000000000000003	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	14	0.35000000000000003	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	14	0.35000000000000003	No Hit
CTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAA	13	0.325	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	13	0.325	No Hit
ATCGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTA	13	0.325	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	12	0.3	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	12	0.3	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	12	0.3	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	12	0.3	No Hit
CCTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTT	12	0.3	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	12	0.3	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	12	0.3	No Hit
CCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGA	12	0.3	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	11	0.27499999999999997	No Hit
GTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACAT	11	0.27499999999999997	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	11	0.27499999999999997	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	11	0.27499999999999997	No Hit
TCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	11	0.27499999999999997	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	11	0.27499999999999997	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	11	0.27499999999999997	No Hit
CCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTT	11	0.27499999999999997	No Hit
GTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCA	11	0.27499999999999997	No Hit
GCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAAT	10	0.25	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	10	0.25	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	10	0.25	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	10	0.25	No Hit
GTTCTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTAT	10	0.25	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	10	0.25	No Hit
CTCGGAACTTCTTTTCATTTCAAACCTTTGAATAAATTCACAATCTTCAG	9	0.22499999999999998	No Hit
CTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAA	9	0.22499999999999998	No Hit
GCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAG	9	0.22499999999999998	No Hit
GTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAAT	9	0.22499999999999998	No Hit
GTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCT	9	0.22499999999999998	No Hit
CTTCAGTTTGAAGCATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCA	9	0.22499999999999998	No Hit
CTACTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCAT	9	0.22499999999999998	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	9	0.22499999999999998	No Hit
ACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	9	0.22499999999999998	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	9	0.22499999999999998	No Hit
CCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAG	9	0.22499999999999998	No Hit
ACCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATT	9	0.22499999999999998	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	9	0.22499999999999998	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	9	0.22499999999999998	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	9	0.22499999999999998	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	9	0.22499999999999998	No Hit
GATTGTTTGAAATTGTTTTAAATCCAGACATTTGGCTTCATTCTATCTAG	8	0.2	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	8	0.2	No Hit
GCCTTTTCCTTTTAACATGTTTAGGTACATACCAATGAGAAAAGCAATTT	8	0.2	No Hit
CCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGA	8	0.2	No Hit
GTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAGAGTCT	8	0.2	No Hit
GCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCAT	8	0.2	No Hit
CTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCG	8	0.2	No Hit
GTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAG	8	0.2	No Hit
GCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAA	8	0.2	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	8	0.2	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	8	0.2	No Hit
GGACTAAGGTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATT	8	0.2	No Hit
CTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTC	8	0.2	No Hit
GCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGT	8	0.2	No Hit
GTCAAATTCATAATTCTTAAATTTTTAAGCAATTTGTAAAAATGGTTTCC	8	0.2	No Hit
GACTATTTTAACCTTATTACTTTTAAACTAATTCTTTCAAACTGTACTTT	8	0.2	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	8	0.2	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	7	0.17500000000000002	No Hit
CACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAGGTTT	7	0.17500000000000002	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	7	0.17500000000000002	No Hit
CTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATGAA	7	0.17500000000000002	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	7	0.17500000000000002	No Hit
GGCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	7	0.17500000000000002	No Hit
CTGTATTCCTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCAGT	7	0.17500000000000002	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	7	0.17500000000000002	No Hit
GCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGG	7	0.17500000000000002	No Hit
ATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCC	7	0.17500000000000002	No Hit
GCTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCAC	7	0.17500000000000002	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	7	0.17500000000000002	No Hit
CTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAACC	7	0.17500000000000002	No Hit
CCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGAC	7	0.17500000000000002	No Hit
TGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCG	7	0.17500000000000002	No Hit
CTTCTCTTGCTTCAGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTC	7	0.17500000000000002	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	7	0.17500000000000002	No Hit
TCTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGA	7	0.17500000000000002	No Hit
AGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAA	6	0.15	No Hit
CTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGT	6	0.15	No Hit
CCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGG	6	0.15	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	6	0.15	No Hit
GTGGGCCACCACGCCCGGCAGACCAATCCTTTTAACATGGACATATTGTT	6	0.15	No Hit
CTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCT	6	0.15	No Hit
CCATGGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTT	6	0.15	No Hit
ATGAGATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCT	6	0.15	No Hit
CGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATT	6	0.15	No Hit
GTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGG	6	0.15	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	6	0.15	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	6	0.15	No Hit
CTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCTGTATCTCAAA	6	0.15	No Hit
CCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTC	6	0.15	No Hit
GGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTC	6	0.15	No Hit
GGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATG	6	0.15	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	6	0.15	No Hit
TTCAGTTTGAAGCATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCAA	6	0.15	No Hit
GGTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTC	6	0.15	No Hit
GCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTAT	6	0.15	No Hit
CGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAA	6	0.15	No Hit
CTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTC	6	0.15	No Hit
GGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCA	6	0.15	No Hit
CTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAGT	6	0.15	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	5	0.125	No Hit
TAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTTTT	5	0.125	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	5	0.125	No Hit
GCCTTCCATCAGTTCTTTCCCCCAAGCAGCTAACTTGGCGATAATTCTTG	5	0.125	No Hit
GCCTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAA	5	0.125	No Hit
GCCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATT	5	0.125	No Hit
GCCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTC	5	0.125	No Hit
TTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAG	5	0.125	No Hit
GGGATATATATATAATGTTCTAACCAGTCCTTATATTAAATAATTTTCAC	5	0.125	No Hit
CTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAAAAGA	5	0.125	No Hit
GGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAG	5	0.125	No Hit
ACCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACAC	5	0.125	No Hit
GCTCTTGCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGAT	5	0.125	No Hit
GTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGC	5	0.125	No Hit
GGTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGAT	5	0.125	No Hit
GGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTA	5	0.125	No Hit
GGCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTAC	5	0.125	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
GGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACA	5	0.125	No Hit
GAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATATTACCTAGGT	5	0.125	No Hit
CCTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAG	5	0.125	No Hit
GGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATT	5	0.125	No Hit
CTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATTTCT	5	0.125	No Hit
GAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACG	5	0.125	No Hit
TGCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATA	5	0.125	No Hit
TGGTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAAC	5	0.125	No Hit
CTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCT	5	0.125	No Hit
GGTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCA	5	0.125	No Hit
GTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATC	5	0.125	No Hit
CCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGC	5	0.125	No Hit
GTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATT	5	0.125	No Hit
GGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAA	5	0.125	No Hit
CCCCCAAGCAGCTAACTTGGCGATAATTCTTGCTGCCATATGAACAGTAA	5	0.125	No Hit
TACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTG	5	0.125	No Hit
CTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCAAC	10	0.006973645	144.0	5
TTCAACC	10	0.006973645	144.0	6
CAACCAG	10	0.006973645	144.0	8
CTCTTCA	10	0.006973645	144.0	3
TCAACCA	10	0.006973645	144.0	7
AACCAGC	10	0.006973645	144.0	9
>>END_MODULE
SRR32233444 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233444_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0435	37.0	37.0	37.0	37.0	37.0
2	36.11075	37.0	37.0	37.0	37.0	37.0
3	36.31	37.0	37.0	37.0	37.0	37.0
4	36.22	37.0	37.0	37.0	37.0	37.0
5	36.215	37.0	37.0	37.0	37.0	37.0
6	36.286	37.0	37.0	37.0	37.0	37.0
7	36.352	37.0	37.0	37.0	37.0	37.0
8	36.274	37.0	37.0	37.0	37.0	37.0
9	36.4085	37.0	37.0	37.0	37.0	37.0
10-14	36.2779	37.0	37.0	37.0	37.0	37.0
15-19	36.2461	37.0	37.0	37.0	37.0	37.0
20-24	36.2303	37.0	37.0	37.0	37.0	37.0
25-29	36.1667	37.0	37.0	37.0	37.0	37.0
30-34	36.1337	37.0	37.0	37.0	37.0	37.0
35-39	36.1283	37.0	37.0	37.0	37.0	37.0
40-44	36.1216	37.0	37.0	37.0	37.0	37.0
45-49	36.06869999999999	37.0	37.0	37.0	37.0	37.0
50-54	36.0167	37.0	37.0	37.0	37.0	37.0
55-59	35.9365	37.0	37.0	37.0	37.0	37.0
60-64	35.928900000000006	37.0	37.0	37.0	37.0	37.0
65-69	35.8806	37.0	37.0	37.0	37.0	37.0
70-74	35.835899999999995	37.0	37.0	37.0	37.0	37.0
75-79	35.821400000000004	37.0	37.0	37.0	37.0	37.0
80-84	35.7821	37.0	37.0	37.0	37.0	37.0
85-89	35.665	37.0	37.0	37.0	37.0	37.0
90-94	35.6408	37.0	37.0	37.0	37.0	37.0
95-99	35.5843	37.0	37.0	37.0	37.0	37.0
100-104	35.4568	37.0	37.0	37.0	34.6	37.0
105-109	35.4203	37.0	37.0	37.0	37.0	37.0
110-114	35.3014	37.0	37.0	37.0	29.8	37.0
115-119	35.2698	37.0	37.0	37.0	29.8	37.0
120-124	35.1057	37.0	37.0	37.0	27.4	37.0
125-129	34.9662	37.0	37.0	37.0	25.0	37.0
130-134	34.911500000000004	37.0	37.0	37.0	25.0	37.0
135-139	34.8633	37.0	37.0	37.0	25.0	37.0
140-144	34.6355	37.0	37.0	37.0	25.0	37.0
145-149	34.485	37.0	37.0	37.0	25.0	37.0
150	34.133	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	2.0
19	1.0
20	1.0
21	1.0
22	2.0
23	1.0
24	1.0
25	5.0
26	5.0
27	5.0
28	18.0
29	32.0
30	34.0
31	40.0
32	64.0
33	128.0
34	326.0
35	1018.0
36	2244.0
37	70.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.300000000000004	24.85	15.4	27.450000000000003
2	19.079769942485623	35.55888972243061	32.68317079269817	12.678169542385595
3	20.974999999999998	33.225	25.474999999999998	20.325
4	22.650000000000002	37.95	22.725	16.675
5	22.400000000000002	35.9	25.974999999999998	15.725
6	18.6	40.325	24.5	16.575
7	18.425	22.825	37.85	20.9
8	21.3	24.75	29.875	24.075
9	24.525	24.175	29.025000000000002	22.275
10-14	24.185000000000002	29.48	24.775	21.560000000000002
15-19	22.155	29.275000000000002	28.02	20.549999999999997
20-24	21.9	29.835	27.72	20.544999999999998
25-29	21.64	30.72	27.18	20.46
30-34	21.245	28.925	29.354999999999997	20.474999999999998
35-39	22.425	28.494999999999997	28.999999999999996	20.080000000000002
40-44	21.88	28.799999999999997	28.37	20.95
45-49	21.495	29.635	27.685	21.185000000000002
50-54	22.305	30.380000000000003	26.779999999999998	20.535
55-59	21.32	30.48	28.139999999999997	20.06
60-64	21.349999999999998	30.64	27.779999999999998	20.23
65-69	22.314999999999998	29.94	27.92	19.825
70-74	21.375	29.470000000000002	28.975	20.18
75-79	21.89	29.2	28.64	20.27
80-84	20.880000000000003	29.595	28.549999999999997	20.974999999999998
85-89	21.29	29.365000000000002	28.939999999999998	20.405
90-94	20.415	31.564999999999998	27.775	20.244999999999997
95-99	21.044999999999998	29.89	29.69	19.375
100-104	22.18	30.075000000000003	27.505000000000003	20.24
105-109	22.73	28.87	27.785	20.615
110-114	20.855	30.415	28.189999999999998	20.54
115-119	21.04	28.84	29.310000000000002	20.810000000000002
120-124	21.48	29.270000000000003	29.87	19.38
125-129	22.065	28.994999999999997	28.955	19.985
130-134	21.825	29.609999999999996	28.54	20.025000000000002
135-139	21.709999999999997	29.654999999999998	28.42	20.215
140-144	21.075	30.025000000000002	29.349999999999998	19.55
145-149	21.495	29.995	29.17	19.34
150	22.125	30.225	27.325	20.325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	3.0
20	4.5
21	4.5
22	17.0
23	29.0
24	25.5
25	26.0
26	38.0
27	71.0
28	93.5
29	92.5
30	87.5
31	90.0
32	119.0
33	120.5
34	107.5
35	113.5
36	127.5
37	127.0
38	114.5
39	149.5
40	172.5
41	194.0
42	198.0
43	174.0
44	166.0
45	143.5
46	146.0
47	158.5
48	153.5
49	101.0
50	66.0
51	73.5
52	73.0
53	78.0
54	67.0
55	41.5
56	33.0
57	33.0
58	25.5
59	31.5
60	37.0
61	21.5
62	8.0
63	9.0
64	16.5
65	23.5
66	21.0
67	30.5
68	34.0
69	19.5
70	23.0
71	29.5
72	18.5
73	5.0
74	1.0
75	2.5
76	3.0
77	2.5
78	1.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.909261871180064	31.324999999999996
2	21.156558533145276	22.5
3	9.167842031029618	14.625
4	4.513399153737659	9.6
5	2.4917724494593325	6.625
6	1.5044663845792194	4.8
7	0.7052186177715092	2.625
8	0.7522331922896097	3.2
9	0.23507287259050302	1.125
>10	0.5641748942172073	3.5749999999999997
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	15	0.375	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	14	0.35000000000000003	No Hit
GTTATAGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGT	13	0.325	No Hit
CAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATT	13	0.325	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	13	0.325	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	12	0.3	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	11	0.27499999999999997	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	11	0.27499999999999997	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	11	0.27499999999999997	No Hit
AAAAGAAGTTCCGAGGACAAGCAAGAGATGCTTCAAACTGAAGGCAGCCA	10	0.25	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	10	0.25	No Hit
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	10	0.25	No Hit
GAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGC	9	0.22499999999999998	No Hit
CAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTG	9	0.22499999999999998	No Hit
CAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGA	9	0.22499999999999998	No Hit
TTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAA	9	0.22499999999999998	No Hit
GGCCCTTCTATTGGGAGACCAGGCTTTTTTTCCCTTCTATGTAATTTTTC	9	0.22499999999999998	No Hit
ATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACT	8	0.2	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	8	0.2	No Hit
AAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTG	8	0.2	No Hit
CAAATGTAGTGTGTATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTG	8	0.2	No Hit
CGCCAAGTTAGCTGCTTGGGGGAAAGAACTGATGGAAGGCAGTGACTTAA	8	0.2	No Hit
TTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATATTTTAT	8	0.2	No Hit
AAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATA	8	0.2	No Hit
TGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCC	8	0.2	No Hit
TATAGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAA	8	0.2	No Hit
GGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACA	8	0.2	No Hit
CAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAG	8	0.2	No Hit
GTTCAAGATGAGCAATCAGACTTATGTTTTATTTTTATCTGGGTAACATA	8	0.2	No Hit
GAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCC	8	0.2	No Hit
GAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCC	8	0.2	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	8	0.2	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	8	0.2	No Hit
AAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATC	7	0.17500000000000002	No Hit
AAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAA	7	0.17500000000000002	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	7	0.17500000000000002	No Hit
GGACATTCGAGGTGCTGTGGATGCTGCTGTCCCAACTAACATCATTGCGG	7	0.17500000000000002	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	7	0.17500000000000002	No Hit
CCCGAGGCTATCCAGGTCTGTGGGAAACATTCAAAGTCATAAAGTTTAGA	7	0.17500000000000002	No Hit
GGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGGGCATG	7	0.17500000000000002	No Hit
GTTTAGATGACCAAGATGGACATTCGAGGTGCTGTGGATGCTGCTGTCCC	7	0.17500000000000002	No Hit
GAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATT	7	0.17500000000000002	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	7	0.17500000000000002	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	7	0.17500000000000002	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	7	0.17500000000000002	No Hit
AACTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACT	7	0.17500000000000002	No Hit
AGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCA	7	0.17500000000000002	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	7	0.17500000000000002	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	6	0.15	No Hit
TGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCC	6	0.15	No Hit
CACAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAA	6	0.15	No Hit
ACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAAA	6	0.15	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	6	0.15	No Hit
GATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACA	6	0.15	No Hit
ATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCT	6	0.15	No Hit
GAAACATTCAAAGTCATAAAGTTTAGATGACCAAGATGGACATTCGAGGT	6	0.15	No Hit
CTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACA	6	0.15	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	6	0.15	No Hit
CTGAGTGAGGCTCGCAACGTCTCCCACGGAGTCGCCTTCGTTCTGCTCTG	6	0.15	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	6	0.15	No Hit
CTAATGTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCC	6	0.15	No Hit
AATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAA	6	0.15	No Hit
CAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCA	6	0.15	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	6	0.15	No Hit
CATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCG	6	0.15	No Hit
TTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTTCAGGTAAGATGTAA	6	0.15	No Hit
GAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTG	6	0.15	No Hit
ATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCA	6	0.15	No Hit
CTCGTTTCCTTGCAAGTGAAATTTGTAAACAACATACATCTCATGGGTTG	6	0.15	No Hit
GTTAGACTATAGTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTG	6	0.15	No Hit
CAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGT	6	0.15	No Hit
AACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAA	6	0.15	No Hit
AAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCT	6	0.15	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	6	0.15	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	6	0.15	No Hit
TTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCA	6	0.15	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	6	0.15	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	6	0.15	No Hit
GCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAG	6	0.15	No Hit
GAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGGAACAATCTCTTCAA	6	0.15	No Hit
ATAAATTGCTTTTCTCATTGGTATGTACCTAAACATGTTAAAAGGAAAAG	5	0.125	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	5	0.125	No Hit
GAGAAACCCTGTCTCGGAAACAAACAAACAAACAAACCGAAACACAGACA	5	0.125	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	5	0.125	No Hit
CTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGT	5	0.125	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	5	0.125	No Hit
GGACTTATTAAATCGGCCATTCGTGATAATAACCCAGTGGTAATGCTAGA	5	0.125	No Hit
CAGTGACTTAAATTACTATTTCAATTGGATAAAAACTCAGCTGAGTTCAC	5	0.125	No Hit
GTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATTACTTAAGTTT	5	0.125	No Hit
ATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTAT	5	0.125	No Hit
GGATTATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACT	5	0.125	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	5	0.125	No Hit
TTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATG	5	0.125	No Hit
CAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATA	5	0.125	No Hit
TAGAGTTATAGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTT	5	0.125	No Hit
TCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATG	5	0.125	No Hit
CTATAATAGCTAAGAATCAGATGGTATTACATTATCCCTAATAATGGGGA	5	0.125	No Hit
ATCTGATGACGCACATCTCCAAAGAACAGACAGTGCAGTACATACTAACC	5	0.125	No Hit
ATCAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGT	5	0.125	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	5	0.125	No Hit
CACAGAGAAACCCTGTCTCGGAAACAAACAAACAAACAAACCGAAACACA	5	0.125	No Hit
GAAAGAACTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAA	5	0.125	No Hit
TGAATGTGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAA	5	0.125	No Hit
GTTGAATATGTTGCAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTT	5	0.125	No Hit
TAAACATGTTAAAAGGAAAAGGCATAATATAAATTTTTAGAGTTACCAAA	5	0.125	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	5	0.125	No Hit
CTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAA	5	0.125	No Hit
CTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAG	5	0.125	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	5	0.125	No Hit
TAAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATAT	5	0.125	No Hit
TGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAGACATAGC	5	0.125	No Hit
GATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGAC	5	0.125	No Hit
CAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATA	5	0.125	No Hit
GTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCCAGGGCTACACA	5	0.125	No Hit
CAAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAAT	5	0.125	No Hit
GTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATA	5	0.125	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	5	0.125	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	5	0.125	No Hit
CAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAA	5	0.125	No Hit
CTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCC	5	0.125	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	5	0.125	No Hit
GGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCA	5	0.125	No Hit
CTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCAGTCCT	5	0.125	No Hit
GTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGACTGACC	5	0.125	No Hit
ATTATTTTGAAATTTTCATCTTAAGTAAAGGAGCTTTTGATAATCAGTCT	5	0.125	No Hit
GAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGA	5	0.125	No Hit
GCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCC	5	0.125	No Hit
CTTCAGCCTGTGCCCATAGTATTCAGGGGGCCCAATGGCGCCTCAGCAGG	5	0.125	No Hit
GTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGCTG	5	0.125	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	5	0.125	No Hit
ATCAAAGTTGTGCACACAATTTGAACTAGCATATTACTTTACTCTTAATT	5	0.125	No Hit
AGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGG	5	0.125	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGACAT	20	0.006139246	28.8	80-84
>>END_MODULE
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
Read 1731035 spots for SRR32233444.sra
Written 1731035 spots for SRR32233444.sra
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
Read 1731031 spots for SRR32233444.sra
Written 1731031 spots for SRR32233444.sra
SRR ids: ['SRR32233444.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gj7mb10b
SRR32233444.sra spots: 13848252
blocks: [[1, 1731031], [1731032, 3462062], [3462063, 5193093], [5193094, 6924124], [6924125, 8655155], [8655156, 10386186], [10386187, 12117217], [12117218, 13848252]]
SRR32233444 file size 4657500
SRR32233444 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233444 SRR32233444_1.fastq SRR32233444_2.fastq
Input file:	SRR32233444_1.fastq
Paired file:	SRR32233444_2.fastq
trimmed:	SRR32233444-trimmed-pair1.fastq, SRR32233444-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:20:58 2026 >> started

Fri Apr 17 04:21:17 2026 >> done (19.545s)
13848252 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       8 ( 0.00%) empty read pairs filtered out after trimming by size control
13848244 (100.00%) read pairs available; of these:
  165840 ( 1.20%) trimmed read pairs available after processing
13682404 (98.80%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 62	       1	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       1	  0.00%
 66	       2	  0.00%
 67	       0	  0.00%
 68	       1	  0.00%
 69	       3	  0.00%
 70	       2	  0.00%
 71	       4	  0.00%
 72	       4	  0.00%
 73	       7	  0.00%
 74	       5	  0.00%
 75	       4	  0.00%
 76	      11	  0.00%
 77	       9	  0.00%
 78	       7	  0.00%
 79	      14	  0.00%
 80	      14	  0.00%
 81	       9	  0.00%
 82	       7	  0.00%
 83	      11	  0.00%
 84	      12	  0.00%
 85	      18	  0.00%
 86	      33	  0.00%
 87	      29	  0.00%
 88	      29	  0.00%
 89	      25	  0.00%
 90	      33	  0.00%
 91	      42	  0.00%
 92	      43	  0.00%
 93	      37	  0.00%
 94	      46	  0.00%
 95	      48	  0.00%
 96	      56	  0.00%
 97	      66	  0.00%
 98	      72	  0.00%
 99	      74	  0.00%
100	      61	  0.00%
101	      75	  0.00%
102	      85	  0.00%
103	      81	  0.00%
104	     102	  0.00%
105	      88	  0.00%
106	      90	  0.00%
107	     112	  0.00%
108	     117	  0.00%
109	     111	  0.00%
110	     135	  0.00%
111	     134	  0.00%
112	     156	  0.00%
113	     138	  0.00%
114	     139	  0.00%
115	     157	  0.00%
116	     192	  0.00%
117	     191	  0.00%
118	     187	  0.00%
119	     196	  0.00%
120	     190	  0.00%
121	     194	  0.00%
122	     210	  0.00%
123	     247	  0.00%
124	     208	  0.00%
125	     239	  0.00%
126	     262	  0.00%
127	     271	  0.00%
128	     242	  0.00%
129	     301	  0.00%
130	     298	  0.00%
131	     303	  0.00%
132	     363	  0.00%
133	     396	  0.00%
134	     395	  0.00%
135	     344	  0.00%
136	     380	  0.00%
137	     451	  0.00%
138	     437	  0.00%
139	     445	  0.00%
140	     469	  0.00%
141	   15625	  0.11%
142	   15827	  0.11%
143	   16449	  0.12%
144	   16787	  0.12%
145	   17388	  0.13%
146	   17977	  0.13%
147	   18100	  0.13%
148	   18590	  0.13%
149	   19126	  0.14%
150	13682404	 98.80%
13848244 reads passed initial QC


criterion=sequence-density
sequence-density=2.62
sequence-density-rank=1
fanout-score=2.83
fanout-score-rank=35
prefix-density=7.42
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=22
fanout-score=58.26
fanout-score-rank=1
prefix-density=2.73
prefix-fanout=1.4
sequence=ATAGGTCTTCACACGAATCCTAGAGTTGAACCGCAGAGACAGCAGGTTGTAGACAATCTCAAAACGGTTCTGCCGAGTTGGG


criterion=sequence-density
sequence-density=7.25
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=37
prefix-density=7.20
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=29
fanout-score=125.51
fanout-score-rank=1
prefix-density=3.16
prefix-fanout=1.0
sequence=CTCCAATTTCAAAAGGACCATGCTTCGTATACATCCCTTCAGTACAATGGACCAGAAGAGAACATCTAGACAGTAGACTATAATAGCTTTTCTGAGGCTAGGCTGTCCTGGGGGCTGGTGTTAAGGGTATCTCAAAGGGTGGGTTGTTGCGACAAGGCCCAGACCCTAAGATGATAACTTTTTCCCACAGGTTCGAACCTCAGATATGGGTGGTTATGCCACATGTCATGACTTCACTGAAG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT -y CTAATAATTGTTT -o SRR32233444 SRR32233444_1.fastq SRR32233444_2.fastq
Input file:	SRR32233444_1.fastq
Paired file:	SRR32233444_2.fastq
trimmed:	SRR32233444-trimmed-pair1.fastq, SRR32233444-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATA
-- paired 3' end adapter sequence (-y):	CTAATAATTGTTT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:22:41 2026 >> started

Fri Apr 17 04:22:58 2026 >> done (16.650s)
9232163 read pairs processed; of these:
    323 ( 0.00%) short read pairs filtered out after trimming by size control
   3461 ( 0.04%) empty read pairs filtered out after trimming by size control
9228379 (99.96%) read pairs available; of these:
     27 ( 0.00%) trimmed read pairs available after processing
9228352 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 54	      1	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      1	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      1	  0.00%
 66	      2	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      1	  0.00%
 70	      1	  0.00%
 71	      4	  0.00%
 72	      2	  0.00%
 73	      2	  0.00%
 74	      2	  0.00%
 75	      2	  0.00%
 76	     10	  0.00%
 77	      6	  0.00%
 78	      4	  0.00%
 79	     12	  0.00%
 80	      7	  0.00%
 81	      6	  0.00%
 82	      5	  0.00%
 83	      5	  0.00%
 84	     11	  0.00%
 85	     15	  0.00%
 86	     22	  0.00%
 87	     20	  0.00%
 88	     17	  0.00%
 89	     15	  0.00%
 90	     20	  0.00%
 91	     29	  0.00%
 92	     28	  0.00%
 93	     26	  0.00%
 94	     34	  0.00%
 95	     25	  0.00%
 96	     39	  0.00%
 97	     41	  0.00%
 98	     51	  0.00%
 99	     53	  0.00%
100	     46	  0.00%
101	     46	  0.00%
102	     54	  0.00%
103	     56	  0.00%
104	     58	  0.00%
105	     60	  0.00%
106	     70	  0.00%
107	     77	  0.00%
108	     86	  0.00%
109	     74	  0.00%
110	     90	  0.00%
111	     84	  0.00%
112	    107	  0.00%
113	     87	  0.00%
114	     92	  0.00%
115	    108	  0.00%
116	    116	  0.00%
117	    117	  0.00%
118	    123	  0.00%
119	    131	  0.00%
120	    135	  0.00%
121	    130	  0.00%
122	    142	  0.00%
123	    169	  0.00%
124	    137	  0.00%
125	    156	  0.00%
126	    185	  0.00%
127	    178	  0.00%
128	    158	  0.00%
129	    207	  0.00%
130	    195	  0.00%
131	    214	  0.00%
132	    239	  0.00%
133	    265	  0.00%
134	    249	  0.00%
135	    217	  0.00%
136	    274	  0.00%
137	    301	  0.00%
138	    303	  0.00%
139	    312	  0.00%
140	    315	  0.00%
141	  10485	  0.11%
142	  10698	  0.12%
143	  10950	  0.12%
144	  11299	  0.12%
145	  11526	  0.12%
146	  11771	  0.13%
147	  11948	  0.13%
148	  12364	  0.13%
149	  12758	  0.14%
150	9117897	 98.80%


criterion=sequence-density
sequence-density=1.14
sequence-density-rank=1
fanout-score=2.17
fanout-score-rank=35
prefix-density=1.17
prefix-fanout=2.1
sequence=GATAATAAATGGTAAGATGAAGTGGAAAGCGAAGAATCGGGTCAAGGTGGCTTTGTCTACTGAGAAGCCCCCTCAAATTCATTCGACTAGGGTTGTTCCAATATATGGGATGGCTGATAGGAGGTTTGTAATAACTGTGGCACCTCAGAATGATATTTGTCCTCATGGAAGGACGTAGCCTATAAATGCTGTGGCTATGACTGCGAACAGTAGAAGTACTCCAATGTTTCAGGTTTCTATAAATGTATATGATCCATAATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGGCTCCGTTTGCGTGTATATATCGGATTAGTCACCCGTAATTTACGTCTCGACAAATGTGTGTTACTGATGAAAAGGCTGTTATTGTATCTGATGTGTAGTGTATGGCTAAGAAAAGACCTGTAATGATTTGGACTATTAGGCAGACTCCTAGAAGGGACCCAAAGTTTCATCATGATGAAATGTTGGATGGGGCAGGTAGGTCA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=32
fanout-score=26.72
fanout-score-rank=1
prefix-density=0.21
prefix-fanout=3.6
sequence=GTATTTTGTTGTTTACAAGGGCCCCCTTCATGAGGTCATATGTTACCAGCTCTGTACAATTGATGATGACATTTCTCATTAGATTAGGGGTCGTCCCTTTCCAAAGTGTTGACAAGCTTTCTGTGGTGGCTATAACTCTGTAAGCATTGTAGGTCCCCGTGTAGCGGGGTTTGATCCCATGCAGATGGCTCTGGGCTTGCATTCTGACCTTCACGACCTCTGTAGGCTGCCCAATGAACACTGCCACACCTCCAGTCATTAAGCCGGCTGAGATCTTGTTTCCGAGAGAGGCAGGTGTTTCTCTCCCTGAAGAGAAGTACTCTTGGACTGAGTCGTAGAGGCCAATCCTGAGTGAGGCAAAGCTGATTTGCCTCTGAATGCCCGCAGGCA


criterion=sequence-density
sequence-density=2.11
sequence-density-rank=1
fanout-score=1.97
fanout-score-rank=40
prefix-density=2.13
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=22.56
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=2.7
sequence=CATCAACTCAATCTCACTTCTATGAAATAAAACTCCAGCAATACTAACTATAATCTCACTGATATTACTATCCCTAGGAGGCCTTCCACCACTAACAGGATTCTTACCAAAATGAATTATCATCACAGAACTTATAAAAAACAACTGTCTAATTATAGCAACACTCATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGCCTAATTTATTCCACTTCACTAACAAT
SRR32233444 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 04:24:02
                             Started mapping on |	Apr 17 04:24:03
                                    Finished on |	Apr 17 04:26:05
       Mapping speed, Million of reads per hour |	408.53

                          Number of input reads |	13844460
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11245510
                        Uniquely mapped reads % |	81.23%
                          Average mapped length |	298.89
                       Number of splices: Total |	9588014
            Number of splices: Annotated (sjdb) |	9519184
                       Number of splices: GT/AG |	9497525
                       Number of splices: GC/AG |	73310
                       Number of splices: AT/AC |	7961
               Number of splices: Non-canonical |	9218
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.70
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.36
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2412237
             % of reads mapped to multiple loci |	17.42%
        Number of reads mapped to too many loci |	10640
             % of reads mapped to too many loci |	0.08%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.25%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	186713	186713	186713
N_multimapping	2412237	2412237	2412237
N_noFeature	413225	10473503	762876
N_ambiguous	593600	38306	161907
UnstrandedReadsAssigned:10238685 PositiveStrandReadsAssigned:733701 NegativeStrandReadsAssigned:10320727
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233444 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233444-trimmed-pair1.fastq
                             SRR32233444-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,844,460 reads, 12,304,699 reads pseudoaligned
[quant] estimated average fragment length: 252.116
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,111 rounds

 109283 SRR32233444.ke.tsv
  52636 SRR32233444.se.tsv
 161919 total
==> SRR32233444.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	5.16667	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	2	0	0
ENSMUST00000178537.1	12	2.33333	0	0
ENSMUST00000178862.1	14	3.16667	0	0
ENSMUST00000179520.1	11	2	0	0
ENSMUST00000179883.1	16	5.16667	0	0
ENSMUST00000195858.1	10	1	0	0
ENSMUST00000179932.1	12	2.33333	0	0

==> SRR32233444.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233444 completed mapping pipeline successfully
