Starting /dee2/code/volunteer_pipeline.sh SRR32233441
    current disk space = 825332268
    free memory = 199595096 
SRR32233441 SRAfilesize
a8adc9da088a040c2407f4dd0b5a0917  SRR32233441.sra
SRR32233441.sra file validated
SRR32233441 is paired end
SRR32233441 is conventional basespace
SRR32233441 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.278	37.0	37.0	37.0	37.0	37.0
2	36.352	37.0	37.0	37.0	37.0	37.0
3	36.5265	37.0	37.0	37.0	37.0	37.0
4	36.556	37.0	37.0	37.0	37.0	37.0
5	36.4795	37.0	37.0	37.0	37.0	37.0
6	36.4045	37.0	37.0	37.0	37.0	37.0
7	36.492	37.0	37.0	37.0	37.0	37.0
8	36.559	37.0	37.0	37.0	37.0	37.0
9	36.5295	37.0	37.0	37.0	37.0	37.0
10-14	36.53775	37.0	37.0	37.0	37.0	37.0
15-19	36.4755	37.0	37.0	37.0	37.0	37.0
20-24	36.5248	37.0	37.0	37.0	37.0	37.0
25-29	36.541	37.0	37.0	37.0	37.0	37.0
30-34	36.462599999999995	37.0	37.0	37.0	37.0	37.0
35-39	36.4394	37.0	37.0	37.0	37.0	37.0
40-44	36.3945	37.0	37.0	37.0	37.0	37.0
45-49	36.3305	37.0	37.0	37.0	37.0	37.0
50-54	36.2785	37.0	37.0	37.0	37.0	37.0
55-59	36.2906	37.0	37.0	37.0	37.0	37.0
60-64	36.213800000000006	37.0	37.0	37.0	37.0	37.0
65-69	36.1521	37.0	37.0	37.0	37.0	37.0
70-74	36.09770000000001	37.0	37.0	37.0	37.0	37.0
75-79	36.122550000000004	37.0	37.0	37.0	37.0	37.0
80-84	36.0826	37.0	37.0	37.0	37.0	37.0
85-89	36.0434	37.0	37.0	37.0	37.0	37.0
90-94	35.931999999999995	37.0	37.0	37.0	37.0	37.0
95-99	35.834500000000006	37.0	37.0	37.0	37.0	37.0
100-104	35.8529	37.0	37.0	37.0	37.0	37.0
105-109	35.8048	37.0	37.0	37.0	37.0	37.0
110-114	35.682399999999994	37.0	37.0	37.0	37.0	37.0
115-119	35.6149	37.0	37.0	37.0	37.0	37.0
120-124	35.5669	37.0	37.0	37.0	37.0	37.0
125-129	35.4337	37.0	37.0	37.0	34.6	37.0
130-134	35.28209999999999	37.0	37.0	37.0	29.8	37.0
135-139	35.249	37.0	37.0	37.0	27.4	37.0
140-144	35.1811	37.0	37.0	37.0	25.0	37.0
145-149	35.05030000000001	37.0	37.0	37.0	25.0	37.0
150	35.1215	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	3.0
27	4.0
28	10.0
29	14.0
30	34.0
31	40.0
32	79.0
33	132.0
34	195.0
35	505.0
36	2891.0
37	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.775	11.0	14.674999999999999	39.550000000000004
2	24.825	13.075000000000001	33.975	28.125
3	25.074999999999996	15.45	24.425	35.05
4	27.1	25.3	27.3	20.3
5	27.900000000000002	28.050000000000004	25.650000000000002	18.4
6	22.725	32.525	27.875	16.875
7	15.45	21.349999999999998	44.05	19.15
8	16.275000000000002	23.325000000000003	37.525	22.875
9	19.725	23.200000000000003	35.05	22.025
10-14	21.173762945914845	30.29469154950718	26.902486616300596	21.62905888827738
15-19	22.955000000000002	28.46	28.675	19.91
20-24	20.915	28.895	29.56	20.630000000000003
25-29	19.52	27.345000000000002	30.064999999999998	23.07
30-34	20.075000000000003	29.044999999999998	26.88	24.0
35-39	21.48	29.92	27.529999999999998	21.07
40-44	21.87	26.915	28.625	22.59
45-49	20.880000000000003	27.29	28.360000000000003	23.47
50-54	21.84	29.549999999999997	27.845	20.765
55-59	19.785	28.185	29.69	22.34
60-64	23.330000000000002	27.744999999999997	28.21	20.715
65-69	22.445	27.205000000000002	29.07	21.279999999999998
70-74	20.09	29.544999999999998	26.919999999999998	23.445
75-79	21.676922307269	28.635749662314275	27.945369953474408	21.741958076942318
80-84	21.565	27.279999999999998	28.970000000000002	22.185
85-89	21.115000000000002	27.785	28.595	22.505
90-94	22.235	28.494999999999997	28.57	20.7
95-99	20.455000000000002	27.625	30.65	21.27
100-104	20.23	27.765	28.810000000000002	23.195
105-109	19.36	26.22	31.595000000000002	22.825
110-114	21.78	27.505000000000003	27.905	22.81
115-119	20.785	28.355000000000004	28.965000000000003	21.895
120-124	22.745	25.7	29.42	22.134999999999998
125-129	21.55	27.66	30.915	19.875
130-134	21.39	28.57	28.615000000000002	21.425
135-139	21.085	27.925	28.53	22.46
140-144	20.615	27.245	27.605	24.535
145-149	22.17	29.49	26.575	21.765
150	20.3	30.85	25.95	22.900000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.5
20	0.5
21	1.0
22	2.0
23	4.0
24	5.0
25	4.0
26	17.0
27	43.0
28	86.5
29	116.0
30	102.5
31	61.5
32	37.0
33	50.5
34	74.0
35	69.0
36	86.0
37	145.5
38	145.0
39	159.0
40	213.5
41	193.5
42	177.0
43	233.0
44	201.5
45	110.0
46	148.0
47	213.5
48	222.5
49	160.5
50	106.5
51	120.0
52	89.0
53	60.0
54	75.0
55	56.0
56	29.0
57	32.0
58	30.5
59	44.0
60	48.0
61	26.0
62	7.5
63	3.0
64	4.5
65	4.0
66	6.5
67	12.5
68	17.0
69	19.5
70	13.0
71	33.5
72	37.0
73	13.5
74	12.5
75	7.5
76	4.0
77	3.5
78	1.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.065
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.055
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.26077428780132	16.175
2	21.694667640613584	14.85
3	9.569028487947406	9.825000000000001
4	6.281957633308985	8.6
5	3.287070854638422	5.625
6	3.214024835646457	6.6000000000000005
7	1.8261504747991233	4.375
8	0.9495982468955442	2.6
9	1.241782322863404	3.8249999999999997
>10	4.6749452154857565	27.525
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	46	1.15	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	45	1.125	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	42	1.05	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	38	0.95	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	30	0.75	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	29	0.7250000000000001	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	29	0.7250000000000001	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	28	0.7000000000000001	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	24	0.6	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	22	0.5499999999999999	No Hit
CTTTCACCTTTACATCCCCGAGGGGGCTCAGGGACTCGGCCCAGGGACAG	22	0.5499999999999999	No Hit
TGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTA	22	0.5499999999999999	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	21	0.525	No Hit
CTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTT	20	0.5	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	20	0.5	No Hit
CCCAGCCATAGCTGCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGA	20	0.5	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	20	0.5	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	19	0.475	No Hit
GTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAA	18	0.44999999999999996	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	18	0.44999999999999996	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	18	0.44999999999999996	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	17	0.42500000000000004	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	17	0.42500000000000004	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	16	0.4	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	16	0.4	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	16	0.4	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	16	0.4	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	16	0.4	No Hit
CCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCG	16	0.4	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	15	0.375	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	15	0.375	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	14	0.35000000000000003	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	14	0.35000000000000003	No Hit
TGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATAC	14	0.35000000000000003	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	13	0.325	No Hit
GGCAATATTAGTTTTCTACGGAAAATCAAAAAAGAACCTTGTTTTTTCTT	13	0.325	No Hit
TGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCAT	13	0.325	No Hit
AGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGT	13	0.325	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	13	0.325	No Hit
CAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCA	13	0.325	No Hit
GAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAG	13	0.325	No Hit
GTCCTTTTGCATCCTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGG	13	0.325	No Hit
CTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACA	12	0.3	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	12	0.3	No Hit
CTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATC	12	0.3	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	12	0.3	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	12	0.3	No Hit
CTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACT	12	0.3	No Hit
CCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATC	12	0.3	No Hit
CCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCT	12	0.3	No Hit
GGCTTTTTTATTATTTTATGTGTATTGAGTATCTTGCCGAAAATCCATGG	11	0.27499999999999997	No Hit
TGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCG	11	0.27499999999999997	No Hit
CTGAATTGTATAATACAAATATATGCATAGACACTTAAAAGGATCATATA	11	0.27499999999999997	No Hit
GCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTT	11	0.27499999999999997	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	11	0.27499999999999997	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	11	0.27499999999999997	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	11	0.27499999999999997	No Hit
AGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAAT	11	0.27499999999999997	No Hit
TGCACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCT	10	0.25	No Hit
ATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGT	10	0.25	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	10	0.25	No Hit
CTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATACT	10	0.25	No Hit
GTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCA	10	0.25	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	10	0.25	No Hit
TGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCC	9	0.22499999999999998	No Hit
TGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAA	9	0.22499999999999998	No Hit
CTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATT	9	0.22499999999999998	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	9	0.22499999999999998	No Hit
TGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAA	9	0.22499999999999998	No Hit
CTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCT	9	0.22499999999999998	No Hit
CTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCC	9	0.22499999999999998	No Hit
CATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCA	9	0.22499999999999998	No Hit
CTCGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAAT	9	0.22499999999999998	No Hit
CACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGA	9	0.22499999999999998	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	9	0.22499999999999998	No Hit
CGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	9	0.22499999999999998	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	9	0.22499999999999998	No Hit
CCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTA	9	0.22499999999999998	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	9	0.22499999999999998	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	9	0.22499999999999998	No Hit
CCTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACA	9	0.22499999999999998	No Hit
CACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAA	8	0.2	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	8	0.2	No Hit
GAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCA	8	0.2	No Hit
ACTGCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACA	8	0.2	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	8	0.2	No Hit
CTCCCTCCACCCGCTTCATGCGCTTCACCTGCTTGCGCCGCCGCGGCCGG	8	0.2	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	8	0.2	No Hit
ATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCT	8	0.2	No Hit
GTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAAT	8	0.2	No Hit
TGTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACAT	8	0.2	No Hit
CTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAG	8	0.2	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	8	0.2	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	8	0.2	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	7	0.17500000000000002	No Hit
CTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAG	7	0.17500000000000002	No Hit
CATATATTTCCCATGAGGGTAACATCATTTAGTCTGTATCATTGGTATGT	7	0.17500000000000002	No Hit
CTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCAT	7	0.17500000000000002	No Hit
GCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACC	7	0.17500000000000002	No Hit
CCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTTTTG	7	0.17500000000000002	No Hit
GTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGACA	7	0.17500000000000002	No Hit
AAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAAG	7	0.17500000000000002	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	7	0.17500000000000002	No Hit
ATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCTCCCT	7	0.17500000000000002	No Hit
GCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAA	7	0.17500000000000002	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	7	0.17500000000000002	No Hit
GTGGGATCAAGACTTTTGGAAGAAAAGACAAACAGGAAAGAGTTATATCT	7	0.17500000000000002	No Hit
CAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAA	7	0.17500000000000002	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	7	0.17500000000000002	No Hit
ATTTTTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAA	7	0.17500000000000002	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	7	0.17500000000000002	No Hit
CTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGTTTAA	7	0.17500000000000002	No Hit
TGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTT	7	0.17500000000000002	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	7	0.17500000000000002	No Hit
CCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTG	7	0.17500000000000002	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	7	0.17500000000000002	No Hit
CCGCTGTTTGTGTTTAGGGTTTCTTAGATGCATTTTCTTACCATTGTAAG	7	0.17500000000000002	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	7	0.17500000000000002	No Hit
CTAGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTAT	7	0.17500000000000002	No Hit
CTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCA	6	0.15	No Hit
AGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAA	6	0.15	No Hit
GCTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCTGCCTC	6	0.15	No Hit
CTTACATCTTACCTGAACACATCTTCACAGAACATACTAGTAATTTTCAA	6	0.15	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	6	0.15	No Hit
GCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATG	6	0.15	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	6	0.15	No Hit
CTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACAT	6	0.15	No Hit
TATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGGT	6	0.15	No Hit
CTCATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGC	6	0.15	No Hit
CTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGT	6	0.15	No Hit
GCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTGGCTGGATTTA	6	0.15	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	6	0.15	No Hit
GGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	6	0.15	No Hit
GAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGC	6	0.15	No Hit
CCTCTGCTAACCTTGTATGCACCGTCATACTGGGCAACTTCTTCCCCAAG	6	0.15	No Hit
TGCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCC	6	0.15	No Hit
GGCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACT	6	0.15	No Hit
GCCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGC	6	0.15	No Hit
GTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAA	6	0.15	No Hit
GTGCTTTTCCTCAAAGCTTTTTATTTTTTTCTGAAAAACATTAGCTTGAA	6	0.15	No Hit
GCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	6	0.15	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	6	0.15	No Hit
TGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGAC	6	0.15	No Hit
CTGCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTC	6	0.15	No Hit
GTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGG	6	0.15	No Hit
CTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGGCAATATTAG	6	0.15	No Hit
CATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTC	6	0.15	No Hit
CTCCCGAGTGCTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCT	6	0.15	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	6	0.15	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	6	0.15	No Hit
CTCTGCTAACCTTGTATGCACCGTCATACTGGGCAACTTCTTCCCCAAGC	6	0.15	No Hit
CCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGC	6	0.15	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	6	0.15	No Hit
TGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCA	6	0.15	No Hit
GGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAAT	6	0.15	No Hit
AATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGG	6	0.15	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	6	0.15	No Hit
ATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACT	6	0.15	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	6	0.15	No Hit
CCCTCAGGTCGGGTCGGCAACCGTCAAATGTCGGGGTAGTTGCAATAGTA	6	0.15	No Hit
CTCGGAATTCCAGGGGCTGACCACTTTTAAACCTGGGCAGTGCCCATACC	6	0.15	No Hit
CGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCT	6	0.15	No Hit
CATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCTGT	6	0.15	No Hit
CCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTG	5	0.125	No Hit
TGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	5	0.125	No Hit
AGCCCCTCAACTGTTCAAGTGGCAGACAAAATAAATTACCATAAATTATA	5	0.125	No Hit
ATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCCC	5	0.125	No Hit
GTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATA	5	0.125	No Hit
CGCAGATTTATTACCTCACATTCAATTCCCTCCTTAGACAATACAGCTGC	5	0.125	No Hit
CTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGT	5	0.125	No Hit
CAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACACCGC	5	0.125	No Hit
GCACCACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATA	5	0.125	No Hit
TCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTAC	5	0.125	No Hit
TGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACATTA	5	0.125	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	5	0.125	No Hit
CTTCTGGAGGGCTAGGTGTGGTGGCTCACACCTCTTTTTTTTTTAAAGAT	5	0.125	No Hit
CTACTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCAT	5	0.125	No Hit
CTACTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAAT	5	0.125	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	5	0.125	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	5	0.125	No Hit
CCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAG	5	0.125	No Hit
TAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTGC	5	0.125	No Hit
CACATCTTCACAGAACATACTAGTAATTTTCAAATGATGTAATAAAACTG	5	0.125	No Hit
GAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCAAAA	5	0.125	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	5	0.125	No Hit
CCCTGGCAGTCCCGATAGTGGGGGCCCATGTGCGGAGACATCAGCGGAGG	5	0.125	No Hit
TCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGG	5	0.125	No Hit
CATCTATGTGAATACAAACAGAAAAATACAGACACTGCATGGAGACTGAA	5	0.125	No Hit
GGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCTT	5	0.125	No Hit
GGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATA	5	0.125	No Hit
CACAGATTTATTACCAGGACTGGAGAGTCTATAACTCTACAGTCTAAAGT	5	0.125	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	5	0.125	No Hit
GGGAAATCCACTTTGTATTCACTGCCCACTTTATTGGAGAGGAATCTTTT	5	0.125	No Hit
TAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGGAG	5	0.125	No Hit
CTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCT	5	0.125	No Hit
CAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGAGC	5	0.125	No Hit
GGGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACT	5	0.125	No Hit
CGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGG	5	0.125	No Hit
GTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAAC	5	0.125	No Hit
GTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTA	5	0.125	No Hit
CTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGA	5	0.125	No Hit
GGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAACTAA	5	0.125	No Hit
GCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTG	5	0.125	No Hit
CATCTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCA	5	0.125	No Hit
CTCCCATATGATCTAGTTCCATGCACGTGCAAAGTCTGTGTTTTCAAATG	5	0.125	No Hit
GCTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCTGTATCTCAA	5	0.125	No Hit
CCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAA	5	0.125	No Hit
AAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATCAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACCT	10	0.006973645	144.0	3
TAAAAAG	10	0.006973645	144.0	9
>>END_MODULE
SRR32233441 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233441_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2295	37.0	37.0	37.0	37.0	37.0
2	36.413	37.0	37.0	37.0	37.0	37.0
3	36.263	37.0	37.0	37.0	37.0	37.0
4	36.254	37.0	37.0	37.0	37.0	37.0
5	36.2985	37.0	37.0	37.0	37.0	37.0
6	36.2755	37.0	37.0	37.0	37.0	37.0
7	36.241	37.0	37.0	37.0	37.0	37.0
8	36.356	37.0	37.0	37.0	37.0	37.0
9	36.322	37.0	37.0	37.0	37.0	37.0
10-14	36.3009	37.0	37.0	37.0	37.0	37.0
15-19	36.251599999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.3121	37.0	37.0	37.0	37.0	37.0
25-29	36.1883	37.0	37.0	37.0	37.0	37.0
30-34	36.118100000000005	37.0	37.0	37.0	37.0	37.0
35-39	36.0587	37.0	37.0	37.0	37.0	37.0
40-44	36.0518	37.0	37.0	37.0	37.0	37.0
45-49	35.925599999999996	37.0	37.0	37.0	37.0	37.0
50-54	35.9352	37.0	37.0	37.0	37.0	37.0
55-59	35.76989999999999	37.0	37.0	37.0	37.0	37.0
60-64	35.7468	37.0	37.0	37.0	37.0	37.0
65-69	35.6937	37.0	37.0	37.0	37.0	37.0
70-74	35.42959999999999	37.0	37.0	37.0	34.6	37.0
75-79	35.450900000000004	37.0	37.0	37.0	34.6	37.0
80-84	35.400800000000004	37.0	37.0	37.0	37.0	37.0
85-89	35.2186	37.0	37.0	37.0	27.4	37.0
90-94	35.213499999999996	37.0	37.0	37.0	29.8	37.0
95-99	35.0137	37.0	37.0	37.0	25.0	37.0
100-104	34.94070000000001	37.0	37.0	37.0	25.0	37.0
105-109	34.8011	37.0	37.0	37.0	25.0	37.0
110-114	34.6449	37.0	37.0	37.0	25.0	37.0
115-119	34.5926	37.0	37.0	37.0	25.0	37.0
120-124	34.4556	37.0	37.0	37.0	25.0	37.0
125-129	34.111000000000004	37.0	37.0	37.0	25.0	37.0
130-134	34.0539	37.0	37.0	37.0	25.0	37.0
135-139	33.846799999999995	37.0	37.0	37.0	25.0	37.0
140-144	33.571299999999994	37.0	37.0	37.0	25.0	37.0
145-149	33.432050000000004	37.0	37.0	37.0	25.0	37.0
150	33.519	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	2.0
23	2.0
24	5.0
25	8.0
26	15.0
27	14.0
28	33.0
29	38.0
30	49.0
31	100.0
32	149.0
33	191.0
34	375.0
35	1052.0
36	1935.0
37	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.624999999999996	20.5	15.525	32.35
2	23.825	31.324999999999996	30.975	13.875000000000002
3	23.0	29.099999999999998	24.2	23.7
4	25.35	34.975	20.849999999999998	18.825
5	23.95	34.75	22.175	19.125
6	20.599999999999998	36.6	25.275	17.525
7	21.0	17.150000000000002	38.275	23.575
8	21.425	21.2	30.55	26.825
9	23.0	23.825	27.275	25.900000000000002
10-14	25.124999999999996	27.505000000000003	23.835	23.535
15-19	23.815	26.26	26.919999999999998	23.005
20-24	23.385	28.605000000000004	26.3	21.709999999999997
25-29	24.104999999999997	27.575	26.045	22.275
30-34	23.195	26.784999999999997	26.810000000000002	23.21
35-39	24.5	25.935000000000002	27.675	21.89
40-44	23.78	27.92	26.215	22.085
45-49	22.67	27.775	25.900000000000002	23.655
50-54	23.07	28.645	26.3	21.985
55-59	22.31	28.325	26.939999999999998	22.425
60-64	24.055	26.945000000000004	26.314999999999998	22.685
65-69	23.974999999999998	27.18	25.919999999999998	22.925
70-74	22.465	27.33	27.1	23.105
75-79	22.89	27.055	28.065	21.990000000000002
80-84	23.244999999999997	27.26	27.339999999999996	22.155
85-89	23.599999999999998	27.650000000000002	26.995	21.755
90-94	21.73	29.025000000000002	26.695	22.55
95-99	23.244999999999997	28.71	28.345	19.7
100-104	22.99	29.195	26.529999999999998	21.285
105-109	23.555	28.33	25.374999999999996	22.74
110-114	22.795	28.64	26.91	21.654999999999998
115-119	22.91	27.405	27.155	22.53
120-124	22.31	28.065	27.07	22.555
125-129	22.6	28.384999999999998	27.26	21.755
130-134	23.485	27.315	26.77	22.43
135-139	22.86	28.044999999999998	27.76	21.335
140-144	22.31	29.904999999999998	26.46	21.325
145-149	22.526126306315316	28.921446072303613	27.02135106755338	21.531076553827692
150	23.5	29.675	27.250000000000004	19.575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	1.5
21	2.0
22	3.5
23	3.5
24	3.0
25	5.5
26	20.5
27	59.0
28	86.0
29	83.5
30	72.5
31	56.0
32	58.0
33	89.5
34	96.0
35	64.5
36	56.0
37	80.5
38	74.0
39	88.0
40	123.5
41	163.0
42	199.0
43	178.5
44	159.0
45	156.5
46	162.5
47	179.5
48	180.0
49	132.0
50	88.0
51	99.5
52	114.5
53	127.5
54	127.5
55	87.5
56	61.0
57	59.0
58	54.5
59	52.5
60	51.0
61	37.5
62	21.5
63	16.5
64	22.5
65	36.0
66	42.5
67	48.5
68	47.5
69	32.5
70	31.5
71	33.5
72	21.0
73	14.0
74	14.5
75	10.0
76	5.0
77	2.0
78	2.0
79	1.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.005
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.550000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.21208384710234	19.55
2	21.763255240443897	17.65
3	10.850801479654747	13.200000000000001
4	6.473489519112208	10.5
5	3.76078914919852	7.625
6	2.651048088779285	6.45
7	1.6029593094944512	4.55
8	1.1097410604192355	3.5999999999999996
9	0.8014796547472256	2.9250000000000003
>10	2.7743526510480887	13.950000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	21	0.525	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	20	0.5	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	18	0.44999999999999996	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	18	0.44999999999999996	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	17	0.42500000000000004	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	17	0.42500000000000004	No Hit
GTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTT	16	0.4	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	16	0.4	No Hit
GAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGC	14	0.35000000000000003	No Hit
CAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGT	14	0.35000000000000003	No Hit
AAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGC	14	0.35000000000000003	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	13	0.325	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	13	0.325	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	13	0.325	No Hit
CAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCA	13	0.325	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	13	0.325	No Hit
CTAAGTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATT	12	0.3	No Hit
GTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGT	12	0.3	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	12	0.3	No Hit
CTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAG	12	0.3	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	11	0.27499999999999997	No Hit
CTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCG	11	0.27499999999999997	No Hit
TATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCC	11	0.27499999999999997	No Hit
TGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTGC	11	0.27499999999999997	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	11	0.27499999999999997	No Hit
CGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGG	11	0.27499999999999997	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	11	0.27499999999999997	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	11	0.27499999999999997	No Hit
GTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCG	11	0.27499999999999997	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	11	0.27499999999999997	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	10	0.25	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	10	0.25	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	10	0.25	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	10	0.25	No Hit
CTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTATTACATCA	10	0.25	No Hit
TGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACAT	10	0.25	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	10	0.25	No Hit
ATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCC	10	0.25	No Hit
CGCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGA	10	0.25	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	10	0.25	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	10	0.25	No Hit
GCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCC	10	0.25	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	10	0.25	No Hit
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	10	0.25	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	10	0.25	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	9	0.22499999999999998	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	9	0.22499999999999998	No Hit
CAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACA	9	0.22499999999999998	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	9	0.22499999999999998	No Hit
GCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTTT	9	0.22499999999999998	No Hit
ATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAA	9	0.22499999999999998	No Hit
GTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGG	9	0.22499999999999998	No Hit
ATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTG	9	0.22499999999999998	No Hit
TAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGC	9	0.22499999999999998	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	9	0.22499999999999998	No Hit
AGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGG	9	0.22499999999999998	No Hit
GAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAA	9	0.22499999999999998	No Hit
AAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAA	9	0.22499999999999998	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	8	0.2	No Hit
AAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTCTAACAGGAT	8	0.2	No Hit
AAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAA	8	0.2	No Hit
GGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTC	8	0.2	No Hit
CAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGG	8	0.2	No Hit
TGCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGAT	8	0.2	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	8	0.2	No Hit
GTTTTGCAAATACATACCAATGATACAGACTAAATGATGTTACCCTCATG	8	0.2	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	8	0.2	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	8	0.2	No Hit
ATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACA	8	0.2	No Hit
CAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTG	8	0.2	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	8	0.2	No Hit
CTCCAGCGCGGATTAGGCGAAGGGTTAGGTCTACCAGGAGAGCAGCGGGA	8	0.2	No Hit
TTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTTCTGTT	8	0.2	No Hit
CTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAATGATA	8	0.2	No Hit
AGCAAAATAAGATAACAAACAGTTTTATTACATCATTTGAAAATTACTAG	8	0.2	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	8	0.2	No Hit
GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCA	7	0.17500000000000002	No Hit
CTTAATTGTAAAATTTTCATTTTTCTTACAATGGTAAGAAAATGCATCTA	7	0.17500000000000002	No Hit
TAAGAATCAGATGGTATTACATTATCCCTAATAATGGGGATAATGCTTTT	7	0.17500000000000002	No Hit
CGTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTC	7	0.17500000000000002	No Hit
CTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAAT	7	0.17500000000000002	No Hit
TAAGCTCCTACCTCCAATTGATTGACATCACTAAGAGGCCTTGAGTAGAA	7	0.17500000000000002	No Hit
GTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACT	7	0.17500000000000002	No Hit
CTTTTAAGTGTCTATGCATATATTTGTATTATACAATTCAGAATATACTA	7	0.17500000000000002	No Hit
CCCAGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGAT	7	0.17500000000000002	No Hit
GAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGACTG	7	0.17500000000000002	No Hit
CCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA	7	0.17500000000000002	No Hit
CTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATATTT	7	0.17500000000000002	No Hit
CAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATC	7	0.17500000000000002	No Hit
CGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGG	7	0.17500000000000002	No Hit
GGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCC	7	0.17500000000000002	No Hit
TGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTT	7	0.17500000000000002	No Hit
TGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCCG	7	0.17500000000000002	No Hit
GAAATATATGATCCTTTTAAGTGTCTATGCATATATTTGTATTATACAAT	7	0.17500000000000002	No Hit
AAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATA	7	0.17500000000000002	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	7	0.17500000000000002	No Hit
ATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAG	7	0.17500000000000002	No Hit
CACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTG	7	0.17500000000000002	No Hit
CCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGA	7	0.17500000000000002	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	7	0.17500000000000002	No Hit
GAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCA	7	0.17500000000000002	No Hit
CGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCA	7	0.17500000000000002	No Hit
TTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGAT	6	0.15	No Hit
GAAAATTAGGCCCTGAGATCTTTGAAAAGATGCCCATTACTAGATACAAA	6	0.15	No Hit
GCAAAATAAGATAACAAACAGTTTTATTACATCATTTGAAAATTACTAGT	6	0.15	No Hit
GGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAA	6	0.15	No Hit
CTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTT	6	0.15	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	6	0.15	No Hit
GGGGATAATGCTTTTGAGTGTCAAAAAGTAATACTGCATTCTTCAATTAA	6	0.15	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	6	0.15	No Hit
CTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGTCAATAG	6	0.15	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	6	0.15	No Hit
GTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAG	6	0.15	No Hit
GTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGA	6	0.15	No Hit
CTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCC	6	0.15	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	6	0.15	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	6	0.15	No Hit
GGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGA	6	0.15	No Hit
TAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGA	6	0.15	No Hit
GGCATCTCCAGGCTAGCTTCCGATCCCTGCCTCAGGGTCGGGGGAAGCGG	6	0.15	No Hit
CAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTG	6	0.15	No Hit
AAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAG	6	0.15	No Hit
CGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCG	6	0.15	No Hit
GTGCGGTTGGCCCCAACACTCCTCCCAAAGTATCTATCAAGAGAATGGTC	6	0.15	No Hit
TGACCATAGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACA	6	0.15	No Hit
TAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAA	6	0.15	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	6	0.15	No Hit
GTGAAGCAACTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAA	6	0.15	No Hit
AGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCC	6	0.15	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	6	0.15	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	6	0.15	No Hit
AAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATT	6	0.15	No Hit
TGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAAGCCC	6	0.15	No Hit
AGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTGAA	6	0.15	No Hit
CGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGC	6	0.15	No Hit
AGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATG	6	0.15	No Hit
ATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCAC	6	0.15	No Hit
AGAAAACTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAG	6	0.15	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	6	0.15	No Hit
AGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCA	6	0.15	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	6	0.15	No Hit
GCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGT	6	0.15	No Hit
CTAGATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTAT	6	0.15	No Hit
GTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATAT	6	0.15	No Hit
CAGGAAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAAT	6	0.15	No Hit
TACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACA	5	0.125	No Hit
TGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCC	5	0.125	No Hit
GTCTCCATGCAGTGTCTGTATTTTTCTGTTTGTATTCACATAGATGATAC	5	0.125	No Hit
GAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTC	5	0.125	No Hit
TGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGA	5	0.125	No Hit
AGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCT	5	0.125	No Hit
GGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGA	5	0.125	No Hit
CTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGG	5	0.125	No Hit
TGCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATC	5	0.125	No Hit
CTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTTG	5	0.125	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	5	0.125	No Hit
CGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCC	5	0.125	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	5	0.125	No Hit
CTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCT	5	0.125	No Hit
CGGGAAAGACGTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGG	5	0.125	No Hit
GTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTCAGC	5	0.125	No Hit
CCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGA	5	0.125	No Hit
CAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACA	5	0.125	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	5	0.125	No Hit
CGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTG	5	0.125	No Hit
TCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGC	5	0.125	No Hit
AAAAAGTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACCA	5	0.125	No Hit
AAAGCACTCTTGAGTCTCACTAATGAGAAGAAAATGCATGATAGACTTTA	5	0.125	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	5	0.125	No Hit
GTTCGTGAAGCTATTAATCAAGGTATGGATGAAGAACTAGAAAGAGATGA	5	0.125	No Hit
GATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGAC	5	0.125	No Hit
TACTATAATAGCTAAGAATCAGATGGTATTACATTATCCCTAATAATGGG	5	0.125	No Hit
GCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGA	5	0.125	No Hit
GAAAATTACTAGTATGTTCTGTGAAGATGTGTTCAGGTAAGATGTAAGTG	5	0.125	No Hit
TATAGTTTTACTCCACAATTGTTCAGTCTCCATGCAGTGTCTGTATTTTT	5	0.125	No Hit
TATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAA	5	0.125	No Hit
TTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTTC	5	0.125	No Hit
ATTCTTTGTCTCAAATTCAGCAAAATAAGATAACAAACAGTTTTATTACA	5	0.125	No Hit
AAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCT	5	0.125	No Hit
CTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCA	5	0.125	No Hit
CTCATGGGAAATATATGATCCTTTTAAGTGTCTATGCATATATTTGTATT	5	0.125	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	5	0.125	No Hit
TGGGTAGATCTCCAGCGCGGATTAGGCGAAGGGTTAGGTCTACCAGGAGA	5	0.125	No Hit
CTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCC	5	0.125	No Hit
CTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAA	5	0.125	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	5	0.125	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	5	0.125	No Hit
CAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAAG	5	0.125	No Hit
CTTTATACACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCA	5	0.125	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	5	0.125	No Hit
GGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAA	5	0.125	No Hit
CCTTGAAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCA	5	0.125	No Hit
TGCAGTGTCTGTATTTTTCTGTTTGTATTCACATAGATGATACTATAATA	5	0.125	No Hit
GTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCGCCC	5	0.125	No Hit
GTATAAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTG	5	0.125	No Hit
GAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACA	5	0.125	No Hit
TTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGT	5	0.125	No Hit
GCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAG	5	0.125	No Hit
TGGGAGACCAGGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAA	5	0.125	No Hit
ATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATGCCT	5	0.125	No Hit
GAGATGGGTCTTCCCTACCAGGGACACGACTGCGGAGTGAACCTCTCAGA	5	0.125	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	5	0.125	No Hit
CAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCA	5	0.125	No Hit
GTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACCAGGCTT	5	0.125	No Hit
AGCTTGTGAATGGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTAT	5	0.125	No Hit
CGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
Read 3460243 spots for SRR32233441.sra
Written 3460243 spots for SRR32233441.sra
2026-04-17T04:44:03 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
Read 3460236 spots for SRR32233441.sra
Written 3460236 spots for SRR32233441.sra
SRR ids: ['SRR32233441.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_rc5240ks
SRR32233441.sra spots: 27681895
blocks: [[1, 3460236], [3460237, 6920472], [6920473, 10380708], [10380709, 13840944], [13840945, 17301180], [17301181, 20761416], [20761417, 24221652], [24221653, 27681895]]
SRR32233441 file size 9331764
SRR32233441 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233441 SRR32233441_1.fastq SRR32233441_2.fastq
Input file:	SRR32233441_1.fastq
Paired file:	SRR32233441_2.fastq
trimmed:	SRR32233441-trimmed-pair1.fastq, SRR32233441-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:45:47 2026 >> started

Fri Apr 17 04:46:59 2026 >> done (72.068s)
27681895 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       5 ( 0.00%) empty read pairs filtered out after trimming by size control
27681890 (100.00%) read pairs available; of these:
  189616 ( 0.68%) trimmed read pairs available after processing
27492274 (99.32%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 58	       1	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       3	  0.00%
 67	       3	  0.00%
 68	       3	  0.00%
 69	       4	  0.00%
 70	       9	  0.00%
 71	       5	  0.00%
 72	       8	  0.00%
 73	       6	  0.00%
 74	       4	  0.00%
 75	       8	  0.00%
 76	       5	  0.00%
 77	      10	  0.00%
 78	       8	  0.00%
 79	      13	  0.00%
 80	      14	  0.00%
 81	      13	  0.00%
 82	      25	  0.00%
 83	      19	  0.00%
 84	      33	  0.00%
 85	      20	  0.00%
 86	      24	  0.00%
 87	      26	  0.00%
 88	      27	  0.00%
 89	      30	  0.00%
 90	      35	  0.00%
 91	      35	  0.00%
 92	      54	  0.00%
 93	      39	  0.00%
 94	      49	  0.00%
 95	      44	  0.00%
 96	      47	  0.00%
 97	      59	  0.00%
 98	      68	  0.00%
 99	      55	  0.00%
100	      72	  0.00%
101	      73	  0.00%
102	      99	  0.00%
103	      96	  0.00%
104	     104	  0.00%
105	     108	  0.00%
106	     109	  0.00%
107	     129	  0.00%
108	     137	  0.00%
109	     134	  0.00%
110	     150	  0.00%
111	     163	  0.00%
112	     164	  0.00%
113	     171	  0.00%
114	     184	  0.00%
115	     196	  0.00%
116	     184	  0.00%
117	     202	  0.00%
118	     223	  0.00%
119	     209	  0.00%
120	     216	  0.00%
121	     229	  0.00%
122	     267	  0.00%
123	     272	  0.00%
124	     301	  0.00%
125	     264	  0.00%
126	     315	  0.00%
127	     328	  0.00%
128	     326	  0.00%
129	     360	  0.00%
130	     405	  0.00%
131	     389	  0.00%
132	     411	  0.00%
133	     449	  0.00%
134	     446	  0.00%
135	     455	  0.00%
136	     528	  0.00%
137	     538	  0.00%
138	     509	  0.00%
139	     546	  0.00%
140	     592	  0.00%
141	   17822	  0.06%
142	   17893	  0.06%
143	   18343	  0.07%
144	   18540	  0.07%
145	   19485	  0.07%
146	   20139	  0.07%
147	   19995	  0.07%
148	   20104	  0.07%
149	   25436	  0.09%
150	27492274	 99.32%
27681890 reads passed initial QC


criterion=sequence-density
sequence-density=5.20
sequence-density-rank=1
fanout-score=2.81
fanout-score-rank=32
prefix-density=14.60
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=39
fanout-score=1138.37
fanout-score-rank=1
prefix-density=15.49
prefix-fanout=2.0
sequence=GTTGTTGATTTAGTCTATTATAT


criterion=sequence-density
sequence-density=14.29
sequence-density-rank=1
fanout-score=1.97
fanout-score-rank=44
prefix-density=14.23
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=46
fanout-score=307.65
fanout-score-rank=1
prefix-density=1.55
prefix-fanout=1.0
sequence=GTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGATTTTAAGGTCTCTAACTTTAACTTAAAAGGTTAACGCTCTTAGCTTCATAGTGAAATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT -y CTAATAATTGTTT -o SRR32233441 SRR32233441_1.fastq SRR32233441_2.fastq
Input file:	SRR32233441_1.fastq
Paired file:	SRR32233441_2.fastq
trimmed:	SRR32233441-trimmed-pair1.fastq, SRR32233441-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATA
-- paired 3' end adapter sequence (-y):	CTAATAATTGTTT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:49:09 2026 >> started

Fri Apr 17 04:49:48 2026 >> done (39.343s)
22648819 read pairs processed; of these:
     313 ( 0.00%) short read pairs filtered out after trimming by size control
    4461 ( 0.02%) empty read pairs filtered out after trimming by size control
22644045 (99.98%) read pairs available; of these:
      73 ( 0.00%) trimmed read pairs available after processing
22643972 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 66	       1	  0.00%
 67	       3	  0.00%
 68	       2	  0.00%
 69	       4	  0.00%
 70	       9	  0.00%
 71	       5	  0.00%
 72	       7	  0.00%
 73	       4	  0.00%
 74	       3	  0.00%
 75	       8	  0.00%
 76	       4	  0.00%
 77	       8	  0.00%
 78	       7	  0.00%
 79	       8	  0.00%
 80	      11	  0.00%
 81	      10	  0.00%
 82	      20	  0.00%
 83	      14	  0.00%
 84	      26	  0.00%
 85	      18	  0.00%
 86	      22	  0.00%
 87	      20	  0.00%
 88	      24	  0.00%
 89	      20	  0.00%
 90	      29	  0.00%
 91	      28	  0.00%
 92	      43	  0.00%
 93	      36	  0.00%
 94	      43	  0.00%
 95	      34	  0.00%
 96	      38	  0.00%
 97	      48	  0.00%
 98	      55	  0.00%
 99	      45	  0.00%
100	      63	  0.00%
101	      66	  0.00%
102	      77	  0.00%
103	      77	  0.00%
104	      82	  0.00%
105	      83	  0.00%
106	      90	  0.00%
107	     104	  0.00%
108	     114	  0.00%
109	     114	  0.00%
110	     118	  0.00%
111	     135	  0.00%
112	     139	  0.00%
113	     138	  0.00%
114	     148	  0.00%
115	     166	  0.00%
116	     156	  0.00%
117	     163	  0.00%
118	     186	  0.00%
119	     171	  0.00%
120	     173	  0.00%
121	     175	  0.00%
122	     215	  0.00%
123	     219	  0.00%
124	     248	  0.00%
125	     219	  0.00%
126	     261	  0.00%
127	     284	  0.00%
128	     256	  0.00%
129	     290	  0.00%
130	     345	  0.00%
131	     314	  0.00%
132	     342	  0.00%
133	     362	  0.00%
134	     360	  0.00%
135	     380	  0.00%
136	     429	  0.00%
137	     446	  0.00%
138	     419	  0.00%
139	     466	  0.00%
140	     484	  0.00%
141	   14256	  0.06%
142	   14624	  0.06%
143	   14929	  0.07%
144	   14918	  0.07%
145	   15877	  0.07%
146	   16526	  0.07%
147	   16310	  0.07%
148	   16372	  0.07%
149	   20710	  0.09%
150	22489789	 99.32%


criterion=sequence-density
sequence-density=1.19
sequence-density-rank=1
fanout-score=2.08
fanout-score-rank=41
prefix-density=1.17
prefix-fanout=2.1
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=40
fanout-score=54.24
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=8.9
sequence=GGCTGAGGAGGGCGCCAATTCCCCACCAGAGGCACCGGGGGCTCATTCTCTGCGCTGGAGAAGAGGCTGCGCAGCTTGGCCATCTGGTCTGAGCTCACAGTCATGGGCTCTTTGAGCAGCAGCCACACAATGCACTCCTCGCAGGGCGGCGTGGTGAAGGAGCCGTGATAGGTCCAATAGTCCCGGCAAGCAGGGAACAGGCATGATGGGTCAAAGTGTGTAAAAGGGGCCTCCTTGCCCTTCGTCTTAATTTTGTCCAGGGCATCAAGAAGAATCTGGAACTCGCCTTTCTCCCGTCCTATCTTCAGGAAAATGCCAACCACAGCGATGCCATCAGGCTGCTTCAGAGCCTCTCCAAAGGTGTTATACTTTGGATTCCAGTGAACCAGGTGAAGCTCAGCAGCGTATTTTACTCCGTCCACGGTGTGCTCAGAGCCGTGGTCATCAGAGGAGCCCCAGTGAA


criterion=sequence-density
sequence-density=2.07
sequence-density-rank=1
fanout-score=1.97
fanout-score-rank=45
prefix-density=2.07
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=37
fanout-score=55.06
fanout-score-rank=1
prefix-density=0.11
prefix-fanout=8.3
sequence=CAGAGCCAGGAAATTGCGTAAGACTTAAAACCTTGTTCCCAGAGGTTCAAATCCTCTCCCTAATAGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCTAACATTGTTGGTCCATACGGCATTTTACAACCATTTGCAGACGCCATAAAATTATTTATAAAAGAACCAATACGCCCTTTAACAACCTCTATATCCTTATTT
SRR32233441 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 04:52:48
                             Started mapping on |	Apr 17 04:52:48
                                    Finished on |	Apr 17 04:56:43
       Mapping speed, Million of reads per hour |	423.99

                          Number of input reads |	27677116
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	22432878
                        Uniquely mapped reads % |	81.05%
                          Average mapped length |	298.85
                       Number of splices: Total |	18573193
            Number of splices: Annotated (sjdb) |	18431762
                       Number of splices: GT/AG |	18399301
                       Number of splices: GC/AG |	141445
                       Number of splices: AT/AC |	14724
               Number of splices: Non-canonical |	17723
                      Mismatch rate per base, % |	0.25%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.63
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.32
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4959343
             % of reads mapped to multiple loci |	17.92%
        Number of reads mapped to too many loci |	14858
             % of reads mapped to too many loci |	0.05%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.95%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	284895	284895	284895
N_multimapping	4959343	4959343	4959343
N_noFeature	919026	20994399	1426508
N_ambiguous	1322406	97545	359580
UnstrandedReadsAssigned:20191446 PositiveStrandReadsAssigned:1340934 NegativeStrandReadsAssigned:20646790
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233441 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233441-trimmed-pair1.fastq
                             SRR32233441-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 27,677,116 reads, 24,732,792 reads pseudoaligned
[quant] estimated average fragment length: 280.08
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,111 rounds

 109283 SRR32233441.ke.tsv
  52636 SRR32233441.se.tsv
 161919 total
==> SRR32233441.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	14	0	0
ENSMUST00000196221.1	9	7	0	0
ENSMUST00000179664.1	11	9	0	0
ENSMUST00000178537.1	12	10	0	0
ENSMUST00000178862.1	14	12	0	0
ENSMUST00000179520.1	11	9	0	0
ENSMUST00000179883.1	16	14	0	0
ENSMUST00000195858.1	10	8	0	0
ENSMUST00000179932.1	12	10	0	0

==> SRR32233441.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233441 completed mapping pipeline successfully
