Starting /dee2/code/volunteer_pipeline.sh SRR32233445
    current disk space = 785902180
    free memory = 196777620 
SRR32233445 SRAfilesize
49fd9ea105dc321af5a91f1f3ace30cf  SRR32233445.sra
SRR32233445.sra file validated
SRR32233445 is paired end
SRR32233445 is conventional basespace
SRR32233445 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.42875	37.0	37.0	37.0	37.0	37.0
2	36.226	37.0	37.0	37.0	37.0	37.0
3	36.486	37.0	37.0	37.0	37.0	37.0
4	36.436	37.0	37.0	37.0	37.0	37.0
5	36.4365	37.0	37.0	37.0	37.0	37.0
6	36.5155	37.0	37.0	37.0	37.0	37.0
7	36.4205	37.0	37.0	37.0	37.0	37.0
8	36.496	37.0	37.0	37.0	37.0	37.0
9	36.484	37.0	37.0	37.0	37.0	37.0
10-14	36.482600000000005	37.0	37.0	37.0	37.0	37.0
15-19	36.4453	37.0	37.0	37.0	37.0	37.0
20-24	36.442400000000006	37.0	37.0	37.0	37.0	37.0
25-29	36.3943	37.0	37.0	37.0	37.0	37.0
30-34	36.3027	37.0	37.0	37.0	37.0	37.0
35-39	36.29985	37.0	37.0	37.0	37.0	37.0
40-44	36.296800000000005	37.0	37.0	37.0	37.0	37.0
45-49	36.24335000000001	37.0	37.0	37.0	37.0	37.0
50-54	36.2038	37.0	37.0	37.0	37.0	37.0
55-59	36.1946	37.0	37.0	37.0	37.0	37.0
60-64	36.13925	37.0	37.0	37.0	37.0	37.0
65-69	36.1687	37.0	37.0	37.0	37.0	37.0
70-74	36.0783	37.0	37.0	37.0	37.0	37.0
75-79	36.007	37.0	37.0	37.0	37.0	37.0
80-84	36.0862	37.0	37.0	37.0	37.0	37.0
85-89	36.0723	37.0	37.0	37.0	37.0	37.0
90-94	35.962900000000005	37.0	37.0	37.0	37.0	37.0
95-99	35.8945	37.0	37.0	37.0	37.0	37.0
100-104	35.8729	37.0	37.0	37.0	37.0	37.0
105-109	35.826100000000004	37.0	37.0	37.0	37.0	37.0
110-114	35.79	37.0	37.0	37.0	37.0	37.0
115-119	35.761700000000005	37.0	37.0	37.0	37.0	37.0
120-124	35.7033	37.0	37.0	37.0	37.0	37.0
125-129	35.6295	37.0	37.0	37.0	37.0	37.0
130-134	35.459300000000006	37.0	37.0	37.0	37.0	37.0
135-139	35.51520000000001	37.0	37.0	37.0	37.0	37.0
140-144	35.369299999999996	37.0	37.0	37.0	37.0	37.0
145-149	35.1235	37.0	37.0	37.0	27.4	37.0
150	35.1695	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	1.0
25	4.0
26	4.0
27	11.0
28	18.0
29	23.0
30	31.0
31	60.0
32	80.0
33	100.0
34	145.0
35	432.0
36	2925.0
37	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78519629907477	8.852213053263316	13.80345086271568	36.55913978494624
2	20.599999999999998	7.5	39.725	32.175
3	23.825	11.0	29.275000000000002	35.9
4	26.174999999999997	16.425	29.849999999999998	27.55
5	29.25	19.075	28.075	23.599999999999998
6	23.425	26.450000000000003	31.724999999999998	18.4
7	15.6	24.95	42.625	16.825000000000003
8	15.925	26.224999999999998	38.85	19.0
9	16.325	30.275000000000002	36.0	17.4
10-14	18.725	31.34	30.605	19.33
15-19	20.29	29.25	30.23	20.23
20-24	18.69	32.75	29.45	19.11
25-29	19.235	30.72	28.895	21.15
30-34	19.555	29.42	27.01	24.015
35-39	18.960948047402372	30.08650432521626	29.956497824891244	20.996049802490123
40-44	19.564999999999998	30.415	28.125	21.895
45-49	19.36596829841492	29.111455572778638	29.67648382419121	21.84609230461523
50-54	19.866986698669866	29.732973297329735	29.027902790279025	21.372137213721373
55-59	18.305	32.205	28.73	20.76
60-64	20.426021301065052	30.101505075253765	28.496424821241064	20.976048802440122
65-69	19.525000000000002	28.694999999999997	29.299999999999997	22.48
70-74	19.53	30.014999999999997	28.444999999999997	22.009999999999998
75-79	19.55	30.42	29.275000000000002	20.755000000000003
80-84	19.625	28.999999999999996	30.735	20.64
85-89	18.7	28.915000000000003	30.44	21.945
90-94	21.959391878375676	29.005801160232046	29.23084616923385	19.80396079215843
95-99	18.38	29.79	31.005	20.825
100-104	18.785	30.330000000000002	29.665000000000003	21.22
105-109	18.51685168516852	28.577857785778576	32.04320432043205	20.862086208620862
110-114	20.82	28.98	28.610000000000003	21.59
115-119	18.84	28.79	32.005	20.365
120-124	21.76217621762176	26.52265226522652	30.368036803680372	21.34713471347135
125-129	19.445	29.310000000000002	31.695	19.55
130-134	19.865	29.104999999999997	30.705	20.325
135-139	19.564999999999998	30.09	27.88	22.465
140-144	18.11	29.020000000000003	31.830000000000002	21.04
145-149	19.7	29.794999999999998	28.03	22.475
150	20.3	30.85	27.375	21.475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	3.0
22	7.0
23	8.0
24	10.0
25	20.0
26	46.5
27	91.5
28	131.5
29	140.0
30	135.5
31	100.0
32	71.0
33	69.0
34	84.0
35	94.5
36	117.0
37	139.0
38	111.5
39	139.5
40	202.5
41	204.5
42	182.0
43	234.0
44	234.0
45	138.5
46	104.0
47	128.0
48	162.5
49	151.5
50	110.0
51	102.5
52	91.5
53	70.5
54	55.0
55	35.5
56	46.0
57	37.5
58	15.0
59	26.5
60	35.5
61	23.5
62	6.5
63	5.0
64	3.0
65	0.5
66	4.0
67	8.0
68	8.5
69	6.0
70	7.5
71	12.0
72	7.5
73	2.5
74	2.5
75	4.0
76	4.0
77	1.5
78	2.5
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.005
40-44	0.0
45-49	0.005
50-54	0.01
55-59	0.0
60-64	0.005
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.02
95-99	0.0
100-104	0.0
105-109	0.01
110-114	0.0
115-119	0.0
120-124	0.01
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.37078651685393	25.974999999999998
2	19.60674157303371	17.45
3	8.426966292134832	11.25
4	4.775280898876404	8.5
5	2.696629213483146	6.0
6	0.9550561797752809	2.55
7	1.1235955056179776	3.5000000000000004
8	0.6741573033707865	2.4
9	0.6741573033707865	2.7
>10	2.640449438202247	18.25
>50	0.05617977528089888	1.425
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	57	1.425	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	32	0.8	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	29	0.7250000000000001	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	29	0.7250000000000001	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	29	0.7250000000000001	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	28	0.7000000000000001	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	27	0.675	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	26	0.65	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	23	0.575	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	22	0.5499999999999999	No Hit
GCTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAA	20	0.5	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	19	0.475	No Hit
GGCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	17	0.42500000000000004	No Hit
TCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	17	0.42500000000000004	No Hit
CTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCT	17	0.42500000000000004	No Hit
CTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATGAA	15	0.375	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	15	0.375	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	15	0.375	No Hit
CCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGA	15	0.375	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	14	0.35000000000000003	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	14	0.35000000000000003	No Hit
CTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAAC	14	0.35000000000000003	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	14	0.35000000000000003	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	14	0.35000000000000003	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	13	0.325	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	13	0.325	No Hit
CCATGGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTT	13	0.325	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	13	0.325	No Hit
GTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTA	13	0.325	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	12	0.3	No Hit
GCTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCTGGTCTTCATGG	12	0.3	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	11	0.27499999999999997	No Hit
GTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGG	11	0.27499999999999997	No Hit
GTTGGTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATT	11	0.27499999999999997	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	11	0.27499999999999997	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	11	0.27499999999999997	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	11	0.27499999999999997	No Hit
GGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTAAAA	10	0.25	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	10	0.25	No Hit
CTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAA	10	0.25	No Hit
CCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACACT	10	0.25	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	10	0.25	No Hit
GCTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCAC	10	0.25	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	10	0.25	No Hit
CCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGAC	10	0.25	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	10	0.25	No Hit
GGTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCA	10	0.25	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	10	0.25	No Hit
CTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAA	9	0.22499999999999998	No Hit
GCCTTTTCCTTTTAACATGTTTAGGTACATACCAATGAGAAAAGCAATTT	9	0.22499999999999998	No Hit
GCTCTTTTTTCTTTTCTTCAGGGTCTTTATCAGTTGATGGTCCATCTAAT	9	0.22499999999999998	No Hit
TTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAG	9	0.22499999999999998	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	9	0.22499999999999998	No Hit
GCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCAT	9	0.22499999999999998	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	9	0.22499999999999998	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	9	0.22499999999999998	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	9	0.22499999999999998	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	9	0.22499999999999998	No Hit
CTTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGT	9	0.22499999999999998	No Hit
GTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAG	9	0.22499999999999998	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	8	0.2	No Hit
GGCAGTATCTAATTTTATACAATTTAACAAGCAAACACTTTTATTCTCCC	8	0.2	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	8	0.2	No Hit
GACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCT	8	0.2	No Hit
CTCCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATC	8	0.2	No Hit
CCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTAT	8	0.2	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	8	0.2	No Hit
GCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	8	0.2	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	8	0.2	No Hit
CCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTT	8	0.2	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	8	0.2	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	8	0.2	No Hit
GTTCTTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACT	7	0.17500000000000002	No Hit
CGGCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATT	7	0.17500000000000002	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	7	0.17500000000000002	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	7	0.17500000000000002	No Hit
ATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGG	7	0.17500000000000002	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	7	0.17500000000000002	No Hit
CCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAGCA	7	0.17500000000000002	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	7	0.17500000000000002	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	7	0.17500000000000002	No Hit
CTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAACC	7	0.17500000000000002	No Hit
ACCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATT	7	0.17500000000000002	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	7	0.17500000000000002	No Hit
CGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAA	7	0.17500000000000002	No Hit
GTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAG	7	0.17500000000000002	No Hit
CTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGGCAATATTAG	7	0.17500000000000002	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	7	0.17500000000000002	No Hit
GCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGT	7	0.17500000000000002	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	7	0.17500000000000002	No Hit
GACTATTTTAACCTTATTACTTTTAAACTAATTCTTTCAAACTGTACTTT	7	0.17500000000000002	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	7	0.17500000000000002	No Hit
TCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCC	6	0.15	No Hit
GTCTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACA	6	0.15	No Hit
GCCTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAA	6	0.15	No Hit
CTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCA	6	0.15	No Hit
CCTGGCTGTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCC	6	0.15	No Hit
CTTATATTAAATAATTTTCACCAGTATTTTGAACATGCTGATGATTTAAA	6	0.15	No Hit
CTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACT	6	0.15	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	6	0.15	No Hit
GTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCT	6	0.15	No Hit
GGTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGAT	6	0.15	No Hit
ACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGC	6	0.15	No Hit
GATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTTGGTT	6	0.15	No Hit
CTTGGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGC	6	0.15	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	6	0.15	No Hit
GGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATT	6	0.15	No Hit
CTTCATTCTATCTAGGACATACCAAAATGATCTTCATATCAATTAGCACC	6	0.15	No Hit
TCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAG	6	0.15	No Hit
GGTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACT	5	0.125	No Hit
GTCCTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCAC	5	0.125	No Hit
TTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTG	5	0.125	No Hit
CCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAG	5	0.125	No Hit
CCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGA	5	0.125	No Hit
GCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAG	5	0.125	No Hit
CAGAAGTTTTGTCAAGATTTTCAAGAGTTCATAGTTCTTCTCATTCAACC	5	0.125	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	5	0.125	No Hit
GGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAAC	5	0.125	No Hit
CATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCAAACCTTTGAATAA	5	0.125	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	5	0.125	No Hit
CTTCGTACTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATA	5	0.125	No Hit
GTGGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTAT	5	0.125	No Hit
GCTATAAGATATTTAAGAAATTATATTTTGAAGCCAGTCACAAGTATTTC	5	0.125	No Hit
GGTCCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCA	5	0.125	No Hit
GGCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGT	5	0.125	No Hit
GCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGG	5	0.125	No Hit
CTTCAGTTTGAAGCATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCA	5	0.125	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	5	0.125	No Hit
CTCCCTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCAT	5	0.125	No Hit
CCAGGATTTGATGAGAGACTTGGCTAGAGAGGTGACTTCTTCATCTGTGC	5	0.125	No Hit
AGGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATC	5	0.125	No Hit
CTACTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCAT	5	0.125	No Hit
CCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	5	0.125	No Hit
CCTCTGCTAACCTTGTATGCACCGTCATACTGGGCAACTTCTTCCCCAAG	5	0.125	No Hit
ACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	5	0.125	No Hit
GGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACA	5	0.125	No Hit
TCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTA	5	0.125	No Hit
GCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATT	5	0.125	No Hit
CCTGTGTATAAGTGCAGTTCTTCTTTTTACATTTGCCACAAGTGAACAAG	5	0.125	No Hit
CCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTCA	5	0.125	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	5	0.125	No Hit
CCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATT	5	0.125	No Hit
CTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCTTC	5	0.125	No Hit
CGGGCTATTCTGTGATGAAATTGCAGGCTCTTTTTTCTTTTCTTCAGGGT	5	0.125	No Hit
TTTAAAGTAAAGCAAAATAGTAAATGATTGTTTGAAATTGTTTTAAATCC	5	0.125	No Hit
GTTCTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTAT	5	0.125	No Hit
CTTTAAAGTAAAGCAAAATAGTAAATGATTGTTTGAAATTGTTTTAAATC	5	0.125	No Hit
CTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCA	5	0.125	No Hit
GGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCTC	5	0.125	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	5	0.125	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	5	0.125	No Hit
CTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAACTGTTT	5	0.125	No Hit
CTTCCTGGGAATCTGGTGAAAGTCCAACGATATCAAACCAAGAAGGCATA	5	0.125	No Hit
CTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACTGGCTG	5	0.125	No Hit
GGGGCATGTGGACAGATGTATTTGATGTGGGGACTTTTGATACCTGCAAA	5	0.125	No Hit
GCTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTCTGTATCTCAA	5	0.125	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0125	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32233445 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233445_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.026	37.0	37.0	37.0	37.0	37.0
2	36.2045	37.0	37.0	37.0	37.0	37.0
3	36.285	37.0	37.0	37.0	37.0	37.0
4	36.199	37.0	37.0	37.0	37.0	37.0
5	36.286	37.0	37.0	37.0	37.0	37.0
6	36.2405	37.0	37.0	37.0	37.0	37.0
7	36.16	37.0	37.0	37.0	37.0	37.0
8	36.3255	37.0	37.0	37.0	37.0	37.0
9	36.2405	37.0	37.0	37.0	37.0	37.0
10-14	36.26619999999999	37.0	37.0	37.0	37.0	37.0
15-19	36.243700000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.251099999999994	37.0	37.0	37.0	37.0	37.0
25-29	36.1759	37.0	37.0	37.0	37.0	37.0
30-34	36.173500000000004	37.0	37.0	37.0	37.0	37.0
35-39	36.112700000000004	37.0	37.0	37.0	37.0	37.0
40-44	36.1722	37.0	37.0	37.0	37.0	37.0
45-49	36.025	37.0	37.0	37.0	37.0	37.0
50-54	36.015	37.0	37.0	37.0	37.0	37.0
55-59	35.9307	37.0	37.0	37.0	37.0	37.0
60-64	35.868399999999994	37.0	37.0	37.0	37.0	37.0
65-69	35.937	37.0	37.0	37.0	37.0	37.0
70-74	35.8949	37.0	37.0	37.0	37.0	37.0
75-79	35.8053	37.0	37.0	37.0	37.0	37.0
80-84	35.7682	37.0	37.0	37.0	37.0	37.0
85-89	35.6873	37.0	37.0	37.0	37.0	37.0
90-94	35.6525	37.0	37.0	37.0	37.0	37.0
95-99	35.571600000000004	37.0	37.0	37.0	37.0	37.0
100-104	35.4722	37.0	37.0	37.0	37.0	37.0
105-109	35.436	37.0	37.0	37.0	37.0	37.0
110-114	35.3913	37.0	37.0	37.0	34.6	37.0
115-119	35.311	37.0	37.0	37.0	32.2	37.0
120-124	35.2374	37.0	37.0	37.0	29.8	37.0
125-129	34.9767	37.0	37.0	37.0	25.0	37.0
130-134	34.979400000000005	37.0	37.0	37.0	25.0	37.0
135-139	34.767999999999994	37.0	37.0	37.0	25.0	37.0
140-144	34.656600000000005	37.0	37.0	37.0	25.0	37.0
145-149	34.6194	37.0	37.0	37.0	25.0	37.0
150	34.152	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	2.0
21	1.0
22	0.0
23	2.0
24	5.0
25	4.0
26	11.0
27	11.0
28	9.0
29	18.0
30	28.0
31	53.0
32	70.0
33	158.0
34	293.0
35	976.0
36	2291.0
37	67.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.324999999999996	25.2	15.125	28.349999999999998
2	20.474999999999998	35.3	30.975	13.25
3	22.125	32.525	24.25	21.099999999999998
4	23.775	37.875	21.625	16.725
5	23.849999999999998	34.75	24.525	16.875
6	20.75	38.7	24.15	16.400000000000002
7	20.225	22.25	38.125	19.400000000000002
8	21.325	24.975	29.675	24.025
9	24.0	25.474999999999998	29.525000000000002	21.0
10-14	24.560000000000002	28.904999999999998	25.035	21.5
15-19	23.325000000000003	28.24	27.155	21.279999999999998
20-24	22.14	30.514999999999997	26.450000000000003	20.895
25-29	23.48	28.720000000000002	27.05	20.75
30-34	21.88	28.18	28.64	21.3
35-39	21.955	29.185	28.599999999999998	20.26
40-44	22.35	29.165000000000003	27.800000000000004	20.685000000000002
45-49	22.215	29.765000000000004	26.505000000000003	21.515
50-54	21.41	30.154999999999998	27.76	20.674999999999997
55-59	20.635	30.91	27.72	20.735
60-64	21.6	29.785	27.91	20.705000000000002
65-69	22.345000000000002	29.485	28.075	20.095
70-74	21.45	29.360000000000003	28.925	20.265
75-79	21.715	29.054999999999996	29.28	19.950000000000003
80-84	21.44	29.01	28.715000000000003	20.835
85-89	21.349999999999998	30.585	27.51	20.555
90-94	20.735	30.95	28.71	19.605
95-99	20.52	29.205	30.575000000000003	19.7
100-104	21.695	29.43	29.060000000000002	19.814999999999998
105-109	22.435	29.770000000000003	28.51	19.285
110-114	21.695	29.659999999999997	28.615000000000002	20.03
115-119	21.275	29.630000000000003	29.39	19.705000000000002
120-124	20.95	30.23	29.095	19.725
125-129	21.240000000000002	29.720000000000002	29.435	19.605
130-134	21.44	28.985	28.63	20.945
135-139	21.18	30.055	29.025000000000002	19.74
140-144	21.66	30.135	29.099999999999998	19.105
145-149	21.105	29.104999999999997	29.625	20.165
150	23.075000000000003	28.825	30.025000000000002	18.075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.5
20	2.5
21	7.0
22	10.0
23	15.5
24	27.5
25	34.0
26	44.0
27	71.0
28	74.5
29	59.0
30	68.5
31	68.5
32	81.5
33	115.0
34	133.0
35	124.5
36	109.5
37	103.0
38	130.5
39	174.0
40	199.0
41	215.0
42	204.0
43	203.0
44	181.0
45	139.0
46	143.0
47	154.5
48	137.0
49	101.5
50	71.0
51	67.5
52	80.0
53	95.5
54	79.0
55	44.5
56	34.5
57	31.0
58	31.0
59	36.0
60	31.5
61	16.0
62	9.0
63	12.5
64	16.0
65	19.0
66	22.0
67	26.5
68	28.0
69	20.0
70	17.5
71	22.0
72	21.5
73	16.5
74	10.0
75	5.0
76	2.0
77	1.5
78	1.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.39999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.056737588652474	35.0
2	20.56737588652482	23.200000000000003
3	8.156028368794328	13.8
4	4.343971631205674	9.8
5	2.304964539007092	6.5
6	0.9308510638297872	3.15
7	0.3546099290780142	1.4000000000000001
8	0.39893617021276595	1.7999999999999998
9	0.31028368794326244	1.575
>10	0.576241134751773	3.775
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	16	0.4	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	15	0.375	No Hit
ATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCT	14	0.35000000000000003	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	12	0.3	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	12	0.3	No Hit
GCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAG	12	0.3	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	10	0.25	No Hit
TCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTC	10	0.25	No Hit
CAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATC	10	0.25	No Hit
GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGT	10	0.25	No Hit
ATTATTTTGAAATTTTCATCTTAAGTAAAGGAGCTTTTGATAATCAGTCT	10	0.25	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	10	0.25	No Hit
GAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCC	10	0.25	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	9	0.22499999999999998	No Hit
TGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCC	9	0.22499999999999998	No Hit
GTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGA	9	0.22499999999999998	No Hit
GTTATAGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGT	9	0.22499999999999998	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	9	0.22499999999999998	No Hit
AAAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTT	9	0.22499999999999998	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	9	0.22499999999999998	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	8	0.2	No Hit
CACAATTTGAACTAGCATATTACTTTACTCTTAATTACTGTGCTCACAAA	8	0.2	No Hit
AAAGAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAAC	8	0.2	No Hit
CTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACTCA	8	0.2	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	8	0.2	No Hit
CACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTAT	8	0.2	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	8	0.2	No Hit
ATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAG	8	0.2	No Hit
AGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCA	8	0.2	No Hit
TTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGCCTTT	7	0.17500000000000002	No Hit
GTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTT	7	0.17500000000000002	No Hit
ATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGT	7	0.17500000000000002	No Hit
GAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTT	7	0.17500000000000002	No Hit
CAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTTGCAGCATTCCG	7	0.17500000000000002	No Hit
TATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAA	7	0.17500000000000002	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	7	0.17500000000000002	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	7	0.17500000000000002	No Hit
ACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACAT	6	0.15	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	6	0.15	No Hit
CATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGT	6	0.15	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	6	0.15	No Hit
CTTTTTATAGCCTAGAACACAAAAATTTTGTTGATCTGTTAATGCATAAA	6	0.15	No Hit
GACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAAAACA	6	0.15	No Hit
CCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGC	6	0.15	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	6	0.15	No Hit
CTGATGACGCACATCTCCAAAGAACAGACAGTGCAGTACATACTAACCAT	6	0.15	No Hit
CCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAACAAACAAA	6	0.15	No Hit
CAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACATT	6	0.15	No Hit
GGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTT	6	0.15	No Hit
GGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGTA	6	0.15	No Hit
GACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATTGGATTTGCTGAA	6	0.15	No Hit
GCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAAT	6	0.15	No Hit
CTACAGTCAGAGCAGCCTCAGACTGCTGCTGCTCGGAGCTGAGCTGACCT	6	0.15	No Hit
CATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAG	6	0.15	No Hit
ATTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGAC	6	0.15	No Hit
CTTCAGCCTGTGCCCATAGTATTCAGGGGGCCCAATGGCGCCTCAGCAGG	6	0.15	No Hit
GTGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATC	6	0.15	No Hit
CAGGTCTGTGGGAAACATTCAAAGTCATAAAGTTTAGATGACCAAGATGG	6	0.15	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	5	0.125	No Hit
ATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACT	5	0.125	No Hit
AGCAGATGGAGTGAACTGTATAATGGGCGTTTTGAGTAACAAGTGTGGTT	5	0.125	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	5	0.125	No Hit
GAGAAACCCTGTCTCGGAAACAAACAAACAAACAAACCGAAACACAGACA	5	0.125	No Hit
CAGAGGCAGGCGGATCTCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCA	5	0.125	No Hit
GTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAACAAAC	5	0.125	No Hit
CATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTTCAGGTAAGA	5	0.125	No Hit
CTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGG	5	0.125	No Hit
TGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATCT	5	0.125	No Hit
GAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAA	5	0.125	No Hit
CAAAAAGTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACC	5	0.125	No Hit
TATATTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATA	5	0.125	No Hit
GGAAAACAATATGTCCATGTTAAAAGGATTGGTCTGCCGGGCGTGGTGGC	5	0.125	No Hit
TCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATG	5	0.125	No Hit
GCCAAGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATT	5	0.125	No Hit
CTTACTATATGTCCGCTGGCCTTCAGCCTGTGCCCATAGTATTCAGGGGG	5	0.125	No Hit
GAAGTTCCGAGGACAAGCAAGAGATGCTTCAAACTGAAGGCAGCCAGTGT	5	0.125	No Hit
AGAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAACCA	5	0.125	No Hit
ATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTT	5	0.125	No Hit
CAATGACAACATTTGTTGTATGTAATGAATGTGGAAATCGGTGGAAGTTC	5	0.125	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	5	0.125	No Hit
CAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTG	5	0.125	No Hit
CACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTC	5	0.125	No Hit
AATAAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAAT	5	0.125	No Hit
CACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACA	5	0.125	No Hit
AGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCC	5	0.125	No Hit
TCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAACAAACAA	5	0.125	No Hit
CAAAAATTTTGTTGATCTGTTAATGCATAAAGATAAATTGCTTTTCTCAT	5	0.125	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	5	0.125	No Hit
CAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCA	5	0.125	No Hit
AACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAA	5	0.125	No Hit
AGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAAAAC	5	0.125	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	5	0.125	No Hit
GTGGTTTTCAACTTCAATACCAAATGATTTTTTCAATATGGCTCCTGGCA	5	0.125	No Hit
GAAATTTGTAAACAACATACATCTCATGGGTTGGTCTTTGTTGTTCACCT	5	0.125	No Hit
GGCCCTTCTATTGGGAGACCAGGCTTTTTTTCCCTTCTATGTAATTTTTC	5	0.125	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	5	0.125	No Hit
GCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCC	5	0.125	No Hit
GAAATATATGATCCTTTTAAGTGTCTATGCATATATTTGTATTATACAAT	5	0.125	No Hit
TAATAGCTAAGAATCAGATGGTATTACATTATCCCTAATAATGGGGATAA	5	0.125	No Hit
AACTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACT	5	0.125	No Hit
GGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAA	5	0.125	No Hit
CAGACATCCTTCAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTT	5	0.125	No Hit
CGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCT	5	0.125	No Hit
CATTCGAGGTGCTGTGGATGCTGCTGTCCCAACTAACATCATTGCGGCCA	5	0.125	No Hit
GACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAAT	5	0.125	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	5	0.125	No Hit
CAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCA	5	0.125	No Hit
CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAA	5	0.125	No Hit
GAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACAGGAACAATCTCTTCAA	5	0.125	No Hit
GTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAGTC	10	0.006973645	144.0	7
TTGGAGC	10	0.006973645	144.0	7
>>END_MODULE
2026-04-17T03:45:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:12 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:13 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:16 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:18 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:19 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:20 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:21 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:25 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:26 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:27 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:28 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
2026-04-17T03:45:29 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:33 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
2026-04-17T03:45:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:46 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
2026-04-17T03:45:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:47 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:48 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T03:45:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
Read 1687154 spots for SRR32233445.sra
Written 1687154 spots for SRR32233445.sra
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
Read 1687148 spots for SRR32233445.sra
Written 1687148 spots for SRR32233445.sra
SRR ids: ['SRR32233445.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fxteoz9n
SRR32233445.sra spots: 13497190
blocks: [[1, 1687148], [1687149, 3374296], [3374297, 5061444], [5061445, 6748592], [6748593, 8435740], [8435741, 10122888], [10122889, 11810036], [11810037, 13497190]]
SRR32233445 file size 4538880
SRR32233445 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233445 SRR32233445_1.fastq SRR32233445_2.fastq
Input file:	SRR32233445_1.fastq
Paired file:	SRR32233445_2.fastq
trimmed:	SRR32233445-trimmed-pair1.fastq, SRR32233445-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 03:46:34 2026 >> started

Fri Apr 17 03:46:59 2026 >> done (24.863s)
13497190 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       9 ( 0.00%) empty read pairs filtered out after trimming by size control
13497181 (100.00%) read pairs available; of these:
  138876 ( 1.03%) trimmed read pairs available after processing
13358305 (98.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 65	       1	  0.00%
 66	       0	  0.00%
 67	       1	  0.00%
 68	       2	  0.00%
 69	       3	  0.00%
 70	       2	  0.00%
 71	       1	  0.00%
 72	       3	  0.00%
 73	       4	  0.00%
 74	       7	  0.00%
 75	       7	  0.00%
 76	      11	  0.00%
 77	       4	  0.00%
 78	      11	  0.00%
 79	       6	  0.00%
 80	      11	  0.00%
 81	      12	  0.00%
 82	      15	  0.00%
 83	      13	  0.00%
 84	      17	  0.00%
 85	      25	  0.00%
 86	      17	  0.00%
 87	      15	  0.00%
 88	      23	  0.00%
 89	      13	  0.00%
 90	      28	  0.00%
 91	      25	  0.00%
 92	      45	  0.00%
 93	      31	  0.00%
 94	      35	  0.00%
 95	      40	  0.00%
 96	      34	  0.00%
 97	      36	  0.00%
 98	      47	  0.00%
 99	      51	  0.00%
100	      54	  0.00%
101	      86	  0.00%
102	      70	  0.00%
103	      61	  0.00%
104	      98	  0.00%
105	      74	  0.00%
106	      93	  0.00%
107	      88	  0.00%
108	      81	  0.00%
109	      97	  0.00%
110	     103	  0.00%
111	     139	  0.00%
112	      98	  0.00%
113	     117	  0.00%
114	     108	  0.00%
115	     130	  0.00%
116	     135	  0.00%
117	     136	  0.00%
118	     148	  0.00%
119	     142	  0.00%
120	     162	  0.00%
121	     203	  0.00%
122	     204	  0.00%
123	     193	  0.00%
124	     209	  0.00%
125	     171	  0.00%
126	     222	  0.00%
127	     218	  0.00%
128	     206	  0.00%
129	     244	  0.00%
130	     259	  0.00%
131	     275	  0.00%
132	     307	  0.00%
133	     278	  0.00%
134	     303	  0.00%
135	     337	  0.00%
136	     350	  0.00%
137	     358	  0.00%
138	     364	  0.00%
139	     355	  0.00%
140	     362	  0.00%
141	   13119	  0.10%
142	   13228	  0.10%
143	   13414	  0.10%
144	   13706	  0.10%
145	   14761	  0.11%
146	   15091	  0.11%
147	   15562	  0.12%
148	   15808	  0.12%
149	   15953	  0.12%
150	13358305	 98.97%
13497181 reads passed initial QC


criterion=sequence-density
sequence-density=2.78
sequence-density-rank=1
fanout-score=2.82
fanout-score-rank=32
prefix-density=7.83
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=21
fanout-score=66.97
fanout-score-rank=1
prefix-density=3.01
prefix-fanout=1.4
sequence=ATAGGTCTTCACACGAATCCTAGAGTTGAACCGCAGAGACAGCAGGTTGTAGACAATCTCAAAACGGTTCTGCCGAGTTGGG


criterion=sequence-density
sequence-density=3.05
sequence-density-rank=1
fanout-score=4.91
fanout-score-rank=27
prefix-density=7.73
prefix-fanout=1.9
sequence=AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=28
fanout-score=125.15
fanout-score-rank=1
prefix-density=3.34
prefix-fanout=1.0
sequence=CTCCAATTTCAAAAGGACCATGCTTCGTATACATCCCTTCAGTACAATGGACCAGAAGAGAACATCTAGACAGTAGACTATAATAGCTTTTCTGAGGCTAGGCTGTCCTGGGGGCTGGTGTTAAGGGTATCTCAAAGGGTGGGTTGTTGCGACAAGGCCCAGACCCTAAGATGATAACTTTTTCCCACAGGTTCGAACCTCAGATATGGGTGGTTATGCCACATGTCATGACTTCACTGAAGCTGT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT -y AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA -o SRR32233445 SRR32233445_1.fastq SRR32233445_2.fastq
Input file:	SRR32233445_1.fastq
Paired file:	SRR32233445_2.fastq
trimmed:	SRR32233445-trimmed-pair1.fastq, SRR32233445-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATA
-- paired 3' end adapter sequence (-y):	AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 03:48:09 2026 >> started

Fri Apr 17 03:48:27 2026 >> done (17.422s)
6748591 read pairs processed; of these:
     30 ( 0.00%) short read pairs filtered out after trimming by size control
   1411 ( 0.02%) empty read pairs filtered out after trimming by size control
6747150 (99.98%) read pairs available; of these:
      9 ( 0.00%) trimmed read pairs available after processing
6747141 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 68	      1	  0.00%
 69	      2	  0.00%
 70	      1	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      2	  0.00%
 74	      4	  0.00%
 75	      4	  0.00%
 76	      4	  0.00%
 77	      2	  0.00%
 78	      9	  0.00%
 79	      2	  0.00%
 80	      6	  0.00%
 81	      5	  0.00%
 82	      4	  0.00%
 83	      9	  0.00%
 84	     14	  0.00%
 85	     13	  0.00%
 86	      7	  0.00%
 87	      5	  0.00%
 88	     13	  0.00%
 89	      4	  0.00%
 90	     14	  0.00%
 91	     15	  0.00%
 92	     18	  0.00%
 93	     12	  0.00%
 94	     18	  0.00%
 95	     22	  0.00%
 96	     12	  0.00%
 97	     17	  0.00%
 98	     25	  0.00%
 99	     22	  0.00%
100	     27	  0.00%
101	     47	  0.00%
102	     29	  0.00%
103	     25	  0.00%
104	     45	  0.00%
105	     42	  0.00%
106	     44	  0.00%
107	     41	  0.00%
108	     41	  0.00%
109	     52	  0.00%
110	     55	  0.00%
111	     64	  0.00%
112	     55	  0.00%
113	     60	  0.00%
114	     58	  0.00%
115	     71	  0.00%
116	     58	  0.00%
117	     61	  0.00%
118	     74	  0.00%
119	     80	  0.00%
120	     78	  0.00%
121	    109	  0.00%
122	     79	  0.00%
123	     87	  0.00%
124	     93	  0.00%
125	     77	  0.00%
126	    112	  0.00%
127	    100	  0.00%
128	     97	  0.00%
129	    123	  0.00%
130	    143	  0.00%
131	    132	  0.00%
132	    167	  0.00%
133	    136	  0.00%
134	    150	  0.00%
135	    170	  0.00%
136	    177	  0.00%
137	    180	  0.00%
138	    169	  0.00%
139	    181	  0.00%
140	    176	  0.00%
141	   6619	  0.10%
142	   6696	  0.10%
143	   6635	  0.10%
144	   6901	  0.10%
145	   7489	  0.11%
146	   7781	  0.12%
147	   7656	  0.11%
148	   7850	  0.12%
149	   7760	  0.12%
150	6677712	 98.97%


criterion=sequence-density
sequence-density=0.81
sequence-density-rank=1
fanout-score=2.85
fanout-score-rank=35
prefix-density=2.31
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=35.82
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=4.0
sequence=TTGCTTGCTTATTAGTGGAAAATCATATCTTTAAAAAAAAACAATAAATCCGACTGACTATTGTAGTGTTTGATGCAAATTTCCATCCAGGCCTCTTCCTTTGGCTCATGCCCTTTCATAAACTCTTGTGGAAGTCACGCCTTTCATAACACATTCCACCACCAGCTTGTCACCATCTCGTTTTCTCTTTATTGTGGTCGACTTTCCATCCCACTTCTGCACCTGCACCAGGGCCCCGCCATCTAGGGTTATG


criterion=sequence-density
sequence-density=2.32
sequence-density-rank=1
fanout-score=1.97
fanout-score-rank=37
prefix-density=2.35
prefix-fanout=1.9
sequence=CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTAATCCTTATCATAATTGCTCTCCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTTCTGCCAATAGAACTTCCAATCCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACAGTAACATCAAACCGACCAGGGTTATTCTATGGCCAATGCTCTG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=30.96
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=5.3
sequence=AAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTGAAATTGACC
SRR32233445 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 03:49:20
                             Started mapping on |	Apr 17 03:49:21
                                    Finished on |	Apr 17 03:51:29
       Mapping speed, Million of reads per hour |	379.57

                          Number of input reads |	13495740
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10833757
                        Uniquely mapped reads % |	80.28%
                          Average mapped length |	298.90
                       Number of splices: Total |	9160365
            Number of splices: Annotated (sjdb) |	9093355
                       Number of splices: GT/AG |	9072878
                       Number of splices: GC/AG |	70353
                       Number of splices: AT/AC |	7729
               Number of splices: Non-canonical |	9405
                      Mismatch rate per base, % |	0.21%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.69
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.33
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2497922
             % of reads mapped to multiple loci |	18.51%
        Number of reads mapped to too many loci |	10066
             % of reads mapped to too many loci |	0.07%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	1.12%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	164061	164061	164061
N_multimapping	2497922	2497922	2497922
N_noFeature	390755	9999903	812522
N_ambiguous	580614	40394	156677
UnstrandedReadsAssigned:9862388 PositiveStrandReadsAssigned:793460 NegativeStrandReadsAssigned:9864558
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233445 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233445-trimmed-pair1.fastq
                             SRR32233445-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,495,740 reads, 11,898,451 reads pseudoaligned
[quant] estimated average fragment length: 255.414
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,107 rounds

 109283 SRR32233445.ke.tsv
  52636 SRR32233445.se.tsv
 161919 total
==> SRR32233445.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	5	0	0
ENSMUST00000196221.1	9	1	0	0
ENSMUST00000179664.1	11	3	0	0
ENSMUST00000178537.1	12	4	0	0
ENSMUST00000178862.1	14	3	0	0
ENSMUST00000179520.1	11	3	0	0
ENSMUST00000179883.1	16	5	0	0
ENSMUST00000195858.1	10	2	0	0
ENSMUST00000179932.1	12	4	0	0

==> SRR32233445.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233445 completed mapping pipeline successfully
