Starting /dee2/code/volunteer_pipeline.sh SRR32233447
    current disk space = 826952804
    free memory = 198514628 
SRR32233447 SRAfilesize
2bb03d469b712c50c4ddd0beeaa3f71d  SRR32233447.sra
SRR32233447.sra file validated
SRR32233447 is paired end
SRR32233447 is conventional basespace
SRR32233447 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.183	37.0	37.0	37.0	37.0	37.0
2	36.17875	37.0	37.0	37.0	37.0	37.0
3	36.2755	37.0	37.0	37.0	37.0	37.0
4	36.2465	37.0	37.0	37.0	37.0	37.0
5	36.3115	37.0	37.0	37.0	37.0	37.0
6	36.264	37.0	37.0	37.0	37.0	37.0
7	36.28225	37.0	37.0	37.0	37.0	37.0
8	36.282	37.0	37.0	37.0	37.0	37.0
9	36.492	37.0	37.0	37.0	37.0	37.0
10-14	36.3488	37.0	37.0	37.0	37.0	37.0
15-19	36.308299999999996	37.0	37.0	37.0	37.0	37.0
20-24	36.2895	37.0	37.0	37.0	37.0	37.0
25-29	36.206599999999995	37.0	37.0	37.0	37.0	37.0
30-34	36.1865	37.0	37.0	37.0	37.0	37.0
35-39	36.16555000000001	37.0	37.0	37.0	37.0	37.0
40-44	36.126000000000005	37.0	37.0	37.0	37.0	37.0
45-49	36.070899999999995	37.0	37.0	37.0	37.0	37.0
50-54	36.03975	37.0	37.0	37.0	37.0	37.0
55-59	36.0423	37.0	37.0	37.0	37.0	37.0
60-64	35.9452	37.0	37.0	37.0	37.0	37.0
65-69	35.930400000000006	37.0	37.0	37.0	37.0	37.0
70-74	35.943799999999996	37.0	37.0	37.0	37.0	37.0
75-79	35.92040000000001	37.0	37.0	37.0	37.0	37.0
80-84	35.87179999999999	37.0	37.0	37.0	37.0	37.0
85-89	35.851800000000004	37.0	37.0	37.0	37.0	37.0
90-94	35.751400000000004	37.0	37.0	37.0	37.0	37.0
95-99	35.75410000000001	37.0	37.0	37.0	37.0	37.0
100-104	35.59439999999999	37.0	37.0	37.0	37.0	37.0
105-109	35.581300000000006	37.0	37.0	37.0	37.0	37.0
110-114	35.5012	37.0	37.0	37.0	37.0	37.0
115-119	35.528200000000005	37.0	37.0	37.0	37.0	37.0
120-124	35.500299999999996	37.0	37.0	37.0	37.0	37.0
125-129	35.353300000000004	37.0	37.0	37.0	37.0	37.0
130-134	35.188	37.0	37.0	37.0	29.8	37.0
135-139	35.2124	37.0	37.0	37.0	29.8	37.0
140-144	35.214800000000004	37.0	37.0	37.0	27.4	37.0
145-149	34.9546	37.0	37.0	37.0	25.0	37.0
150	34.7755	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	1.0
25	4.0
26	8.0
27	17.0
28	26.0
29	34.0
30	74.0
31	62.0
32	88.0
33	124.0
34	191.0
35	474.0
36	2751.0
37	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.125	9.675	13.350000000000001	36.85
2	21.080270067516878	9.727431857964492	37.7344336084021	31.45786446611653
3	23.0	11.675	28.075	37.25
4	22.325	18.5	30.025000000000002	29.15
5	29.725	19.45	27.700000000000003	23.125
6	23.25	27.150000000000002	30.75	18.85
7	17.079269817454364	23.605901475368842	41.2353088272068	18.079519879969993
8	15.975	27.750000000000004	37.574999999999996	18.7
9	15.775	29.075	36.15	19.0
10-14	19.18	30.17	30.895	19.755
15-19	20.674999999999997	29.145	29.92	20.26
20-24	18.86	31.814999999999998	29.599999999999998	19.725
25-29	18.62	29.835	30.595	20.95
30-34	19.305	28.945	28.07	23.68
35-39	20.175043760940234	29.9074768692173	29.18729682420605	20.730182545636406
40-44	20.19	30.29	28.16	21.36
45-49	20.154030806161234	28.055611122224445	30.126025205041007	21.664332866573314
50-54	19.368715922164974	29.133109899454755	29.93347006152769	21.564704116852585
55-59	18.836883688368836	30.688068806880686	29.977997799779978	20.497049704970497
60-64	20.3020302030203	29.292929292929294	28.247824782478247	22.157215721572157
65-69	19.155	28.95	28.835	23.06
70-74	18.85	30.545	28.49	22.115000000000002
75-79	19.505	30.470000000000002	28.720000000000002	21.305
80-84	20.200000000000003	28.965000000000003	30.42	20.415
85-89	17.89178917891789	29.327932793279327	30.598059805980597	22.182218221822183
90-94	21.52	28.52	29.69	20.27
95-99	20.064999999999998	28.895	29.865000000000002	21.175
100-104	19.23	30.404999999999998	29.32	21.044999999999998
105-109	18.709999999999997	26.76	32.379999999999995	22.15
110-114	20.745	29.26	28.615000000000002	21.38
115-119	19.139999999999997	28.04	31.865	20.955
120-124	22.255	26.165	29.5	22.08
125-129	19.24	28.95	31.855	19.955000000000002
130-134	20.47	28.535	30.415	20.580000000000002
135-139	19.445	29.755	28.185	22.615
140-144	18.490000000000002	29.115000000000002	31.169999999999998	21.224999999999998
145-149	19.18	29.439999999999998	28.63	22.75
150	20.424999999999997	29.7	28.599999999999998	21.275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	1.0
21	4.0
22	5.0
23	4.5
24	6.0
25	15.5
26	51.5
27	79.5
28	106.0
29	117.5
30	114.5
31	105.5
32	83.0
33	72.5
34	83.5
35	106.5
36	125.0
37	135.0
38	133.0
39	182.5
40	219.5
41	189.5
42	153.5
43	187.5
44	201.0
45	136.0
46	121.5
47	158.5
48	174.5
49	157.5
50	131.0
51	111.5
52	84.5
53	76.5
54	71.0
55	43.0
56	45.0
57	32.5
58	13.0
59	17.0
60	29.0
61	24.0
62	6.5
63	4.5
64	4.0
65	2.5
66	2.5
67	6.5
68	8.5
69	11.0
70	13.0
71	7.0
72	4.0
73	4.5
74	3.5
75	2.5
76	1.0
77	2.5
78	2.5
79	0.0
80	0.0
81	1.5
82	1.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.025
40-44	0.0
45-49	0.02
50-54	0.045
55-59	0.01
60-64	0.01
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.01
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.04866047020229	27.0
2	20.12028430836523	18.4
3	7.2717331875341715	9.975000000000001
4	4.48332422088573	8.200000000000001
5	2.460360852925096	5.625
6	1.366867140513942	3.75
7	1.2575177692728268	4.025
8	0.7654455986878075	2.8000000000000003
9	1.0934937124111537	4.5
>10	2.1323127392017494	15.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	43	1.075	No Hit
CCCAGATAAAAATAAAACATAAGTCTGATTGCTCATCTTGAACAGTATTT	33	0.8250000000000001	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	28	0.7000000000000001	No Hit
GCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAATACA	27	0.675	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	25	0.625	No Hit
GCTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCTGGTCTTCATGG	23	0.575	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	21	0.525	No Hit
CCCACATTACAGCACACAGTCAATGGAATATTTCTTATACCAAATAAATC	21	0.525	No Hit
GCTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAA	20	0.5	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	20	0.5	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	17	0.42500000000000004	No Hit
GGTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCA	16	0.4	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	16	0.4	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	16	0.4	No Hit
CTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATGAA	15	0.375	No Hit
GTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCT	15	0.375	No Hit
CTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTG	15	0.375	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	15	0.375	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	14	0.35000000000000003	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	14	0.35000000000000003	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	14	0.35000000000000003	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	13	0.325	No Hit
GTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAG	13	0.325	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	12	0.3	No Hit
CTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGT	12	0.3	No Hit
GTCCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCC	12	0.3	No Hit
CCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGAC	12	0.3	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	12	0.3	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	11	0.27499999999999997	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	11	0.27499999999999997	No Hit
CTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAAC	11	0.27499999999999997	No Hit
CTCTGTTTTTGTATTTCATGTCTGTATTCCTTATTTCTTGATATATAGCT	11	0.27499999999999997	No Hit
GCATGGAGACTGAACAATTGTGGAGTAAAACTATAGTCTAACTCACAGAA	11	0.27499999999999997	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	10	0.25	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	10	0.25	No Hit
GCCATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTAT	10	0.25	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	10	0.25	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	10	0.25	No Hit
CTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACTGGCTG	10	0.25	No Hit
GTCCTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCAC	9	0.22499999999999998	No Hit
CTCGGAACTTCTTTTCATTTCAAACCTTTGAATAAATTCACAATCTTCAG	9	0.22499999999999998	No Hit
TTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTG	9	0.22499999999999998	No Hit
CCATGGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTT	9	0.22499999999999998	No Hit
CTCCTTTACTTAAGATGAAAATTTCAAAATAATATGTTACCCAGATAAAA	9	0.22499999999999998	No Hit
CTCCTGTCCGAAGAGCTGCAGCAAGCATCTCCCTACATTTTAATCGCACA	9	0.22499999999999998	No Hit
TCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGG	9	0.22499999999999998	No Hit
GTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGC	9	0.22499999999999998	No Hit
CCCCTCACAGCCTCCACGCCAAACAAGGAGCATCAAGAATCTGTCTGGGT	9	0.22499999999999998	No Hit
CGCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTAC	9	0.22499999999999998	No Hit
GCCTGGTCTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTT	9	0.22499999999999998	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	9	0.22499999999999998	No Hit
CGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAA	9	0.22499999999999998	No Hit
CTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGGCAATATTAG	9	0.22499999999999998	No Hit
GTTCTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTAT	9	0.22499999999999998	No Hit
GGGATCCTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCT	9	0.22499999999999998	No Hit
CCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGA	9	0.22499999999999998	No Hit
CTTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGT	9	0.22499999999999998	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	9	0.22499999999999998	No Hit
CTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATTT	9	0.22499999999999998	No Hit
GTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGGCAATATT	8	0.2	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	8	0.2	No Hit
CCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAG	8	0.2	No Hit
GCTGAATTTGAGACAAAGAATCTAGTTGCTTCACTTACCTACTGTAATAC	8	0.2	No Hit
GGCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	8	0.2	No Hit
GGACAATTCATCAGAAGTATACCTGTGGTGGGCTTTATGAAAAGCTTAGG	8	0.2	No Hit
ATCTGATTCTTAGCTATTATAGTATCATCTATGTGAATACAAACAGAAAA	8	0.2	No Hit
CTACTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCAT	8	0.2	No Hit
GTAAAACTATAGTCTAACTCACAGAACTAAGAAGTCACTTACATCTTACC	8	0.2	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	8	0.2	No Hit
CTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAACC	8	0.2	No Hit
GAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACG	8	0.2	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	8	0.2	No Hit
GTGGGCTTTATGAAAAGCTTAGGAAATTATAACAGAGTTCTATAAAACTA	8	0.2	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	7	0.17500000000000002	No Hit
CCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACACT	7	0.17500000000000002	No Hit
GTCTGTATTCCTTATTTCTTGATATATAGCTTCCTCAATCTGAGATCCCA	7	0.17500000000000002	No Hit
AGTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCT	7	0.17500000000000002	No Hit
GTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATGG	7	0.17500000000000002	No Hit
GGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTG	7	0.17500000000000002	No Hit
CCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATCAT	7	0.17500000000000002	No Hit
CTGACTGTAGTTGTTTACCAAGATATTCCCAGTTGTGCACCATGAGCTTC	7	0.17500000000000002	No Hit
CTCCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATC	7	0.17500000000000002	No Hit
GGCAACTACAGTGATGTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAA	7	0.17500000000000002	No Hit
AGGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATC	7	0.17500000000000002	No Hit
CCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTA	7	0.17500000000000002	No Hit
GTTGGAAAACAGCAAGAAAAGCAACCGTTCAGAAATAACCATAATTTTTG	7	0.17500000000000002	No Hit
GCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCAT	7	0.17500000000000002	No Hit
TTCTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATG	7	0.17500000000000002	No Hit
CTTTAAAGTAAAGCAAAATAGTAAATGATTGTTTGAAATTGTTTTAAATC	7	0.17500000000000002	No Hit
GCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACAGAAAATGT	7	0.17500000000000002	No Hit
CCCGGGGATAATGCCGGACATATTCTCCAACATCGTGAGCAGCAACAGCT	7	0.17500000000000002	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	7	0.17500000000000002	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	7	0.17500000000000002	No Hit
CTCCATTCCAATCTTCCAGATTTAAGTTCTGAACTGTATTCATCAAATGA	7	0.17500000000000002	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	7	0.17500000000000002	No Hit
CCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACT	7	0.17500000000000002	No Hit
GTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACAT	6	0.15	No Hit
CCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACG	6	0.15	No Hit
TTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGAC	6	0.15	No Hit
GGCAGTATCTAATTTTATACAATTTAACAAGCAAACACTTTTATTCTCCC	6	0.15	No Hit
GTTCTTCTCATTCAACCTCACGGCATTTTCTCTCCAAAATTTCTCAGACT	6	0.15	No Hit
ATGAGATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCT	6	0.15	No Hit
TTCCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAG	6	0.15	No Hit
TGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTG	6	0.15	No Hit
GTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAAT	6	0.15	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	6	0.15	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	6	0.15	No Hit
CTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGCAAATAGATCAGGAG	6	0.15	No Hit
GTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCTA	6	0.15	No Hit
GACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCT	6	0.15	No Hit
CTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCC	6	0.15	No Hit
GCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGG	6	0.15	No Hit
GAGAAATAAAAGAAATGATCACTGTGGTTAGTCACAGATGCAAGAGATCC	6	0.15	No Hit
GGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCTC	6	0.15	No Hit
CCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTCA	6	0.15	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	6	0.15	No Hit
CTGTAATACTTAGTATATTCTGAATTGTATAATACAAATATATGCATAGA	6	0.15	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	6	0.15	No Hit
GGCCTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAA	6	0.15	No Hit
TGTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCT	6	0.15	No Hit
CTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCCA	6	0.15	No Hit
AGTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAA	5	0.125	No Hit
CTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACA	5	0.125	No Hit
TGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCAC	5	0.125	No Hit
TCCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCC	5	0.125	No Hit
CCTTCCATCAGTTCTTTCCCCCAAGCAGCTAACTTGGCGATAATTCTTGC	5	0.125	No Hit
GTGGGCCACCACGCCCGGCAGACCAATCCTTTTAACATGGACATATTGTT	5	0.125	No Hit
GTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCCAACGTCCG	5	0.125	No Hit
AATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATCATT	5	0.125	No Hit
GCCATATTGAAAAAATCATTTGGTATTGAAGTTGAAAACCACACTTGTTA	5	0.125	No Hit
TCTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAA	5	0.125	No Hit
TCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCCC	5	0.125	No Hit
TTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAA	5	0.125	No Hit
GCCTTTTCCTTTTAACATGTTTAGGTACATACCAATGAGAAAAGCAATTT	5	0.125	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	5	0.125	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	5	0.125	No Hit
CTCCCAATAGAAGGGCCATTAATTGAAGAATGCAGTATTACTTTTTGACA	5	0.125	No Hit
CCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGA	5	0.125	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	5	0.125	No Hit
TCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTT	5	0.125	No Hit
ATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGG	5	0.125	No Hit
CTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACT	5	0.125	No Hit
GTCTGATTGCTCATCTTGAACAGTATTTGGTTTTAAGCATAACATGTACA	5	0.125	No Hit
GCCTCTTAGTGATGTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTG	5	0.125	No Hit
GGATAATGTAATACCATCTGATTCTTAGCTATTATAGTATCATCTATGTG	5	0.125	No Hit
CCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAGCA	5	0.125	No Hit
GGCTATGTCTGTATCTCAAAATGTCAAAATAAAATATATGACAGAGAGGC	5	0.125	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	5	0.125	No Hit
AGCTGAGTTTTTATCCAATTGAAATAGTAATTTAAGTCACTGCCTTCCAT	5	0.125	No Hit
CTGTCTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCA	5	0.125	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	5	0.125	No Hit
CATTAATTGAAGAATGCAGTATTACTTTTTGACACTCAAAAGCATTATCC	5	0.125	No Hit
GTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAG	5	0.125	No Hit
CCCAGCCATAGCTGCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGA	5	0.125	No Hit
CCTCCCGAGTGCTGGGATTAAAGGCATGCACCACCACGCCCGGCTATGTC	5	0.125	No Hit
GTCAATCAATTGGAGGTAGGAGCTTATCAATGAAGTGCTTGACATCCATC	5	0.125	No Hit
CCTGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTT	5	0.125	No Hit
CTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACT	5	0.125	No Hit
CTCATCTCTTTGAGCTCATCACTAGCCATTTCCTCTGCTGTCATTCTAGC	5	0.125	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	5	0.125	No Hit
CCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTC	5	0.125	No Hit
CCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGTGA	5	0.125	No Hit
CTTCTTTTTACATTTGCCACAAGTGAACAAGTCAGTCTGGGTCCCACCAG	5	0.125	No Hit
CCCCCAAGCAGCTAACTTGGCGATAATTCTTGCTGCCATATGAACAGTAA	5	0.125	No Hit
ACGCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTA	5	0.125	No Hit
TTCCGGAATGCTGCAAGAATGATTCTTGTTACTTTCTCTTTGACAGATTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32233447 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233447_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0635	37.0	37.0	37.0	37.0	37.0
2	36.28525	37.0	37.0	37.0	37.0	37.0
3	36.3335	37.0	37.0	37.0	37.0	37.0
4	36.3535	37.0	37.0	37.0	37.0	37.0
5	36.3365	37.0	37.0	37.0	37.0	37.0
6	36.2365	37.0	37.0	37.0	37.0	37.0
7	36.272	37.0	37.0	37.0	37.0	37.0
8	36.3635	37.0	37.0	37.0	37.0	37.0
9	36.3065	37.0	37.0	37.0	37.0	37.0
10-14	36.356700000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.3305	37.0	37.0	37.0	37.0	37.0
20-24	36.296099999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.2809	37.0	37.0	37.0	37.0	37.0
30-34	36.306799999999996	37.0	37.0	37.0	37.0	37.0
35-39	36.2485	37.0	37.0	37.0	37.0	37.0
40-44	36.1739	37.0	37.0	37.0	37.0	37.0
45-49	36.155499999999996	37.0	37.0	37.0	37.0	37.0
50-54	36.165499999999994	37.0	37.0	37.0	37.0	37.0
55-59	36.0711	37.0	37.0	37.0	37.0	37.0
60-64	36.0418	37.0	37.0	37.0	37.0	37.0
65-69	35.9758	37.0	37.0	37.0	37.0	37.0
70-74	35.9255	37.0	37.0	37.0	37.0	37.0
75-79	35.9917	37.0	37.0	37.0	37.0	37.0
80-84	35.8835	37.0	37.0	37.0	37.0	37.0
85-89	35.778800000000004	37.0	37.0	37.0	37.0	37.0
90-94	35.76899999999999	37.0	37.0	37.0	37.0	37.0
95-99	35.7479	37.0	37.0	37.0	37.0	37.0
100-104	35.7057	37.0	37.0	37.0	37.0	37.0
105-109	35.6997	37.0	37.0	37.0	37.0	37.0
110-114	35.50320000000001	37.0	37.0	37.0	37.0	37.0
115-119	35.45270000000001	37.0	37.0	37.0	37.0	37.0
120-124	35.441900000000004	37.0	37.0	37.0	32.2	37.0
125-129	35.19	37.0	37.0	37.0	27.4	37.0
130-134	35.1096	37.0	37.0	37.0	25.0	37.0
135-139	34.8911	37.0	37.0	37.0	25.0	37.0
140-144	34.797000000000004	37.0	37.0	37.0	25.0	37.0
145-149	34.9288	37.0	37.0	37.0	25.0	37.0
150	34.902	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	0.0
18	1.0
19	0.0
20	2.0
21	2.0
22	3.0
23	3.0
24	3.0
25	5.0
26	5.0
27	6.0
28	14.0
29	15.0
30	42.0
31	51.0
32	83.0
33	115.0
34	228.0
35	714.0
36	2569.0
37	137.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.125	24.8	14.249999999999998	27.825
2	19.72993248312078	35.8589647411853	29.332333083270818	15.078769692423105
3	22.225	33.775	24.125	19.875
4	24.4	36.199999999999996	22.075	17.325
5	23.0	37.775	23.400000000000002	15.825
6	20.375	38.425	24.825	16.375
7	19.900000000000002	22.15	36.375	21.575
8	21.55	26.3	29.65	22.5
9	22.85	24.675	28.975	23.5
10-14	23.945	29.81	24.73	21.515
15-19	22.55	29.195	27.88	20.375
20-24	22.264999999999997	30.385	26.584999999999997	20.765
25-29	22.43	30.665	26.575	20.330000000000002
30-34	22.13	29.065	28.99	19.814999999999998
35-39	21.55	29.244999999999997	28.970000000000002	20.235
40-44	21.675	29.25	28.46	20.615
45-49	22.475	29.970000000000002	26.950000000000003	20.605
50-54	21.335	30.769999999999996	26.745	21.15
55-59	21.05	30.03	28.799999999999997	20.119999999999997
60-64	22.15	30.209999999999997	27.145000000000003	20.495
65-69	22.465	29.695	27.62	20.22
70-74	21.5	30.175	27.965	20.36
75-79	22.115000000000002	29.275000000000002	28.599999999999998	20.01
80-84	21.279999999999998	29.815	27.825	21.08
85-89	22.54	29.45	28.084999999999997	19.925
90-94	21.55	30.61	28.244999999999997	19.595000000000002
95-99	21.505	28.7	30.23	19.564999999999998
100-104	22.189999999999998	29.21	28.105000000000004	20.495
105-109	22.925	28.985	28.13	19.96
110-114	20.985	30.75	27.785	20.48
115-119	21.6	29.630000000000003	28.08	20.69
120-124	21.955	30.34	28.939999999999998	18.765
125-129	21.245	29.21	29.330000000000002	20.215
130-134	22.25	29.599999999999998	27.905	20.244999999999997
135-139	21.98	30.014999999999997	28.044999999999998	19.96
140-144	21.959999999999997	30.055	28.46	19.525000000000002
145-149	21.765	29.53	29.485	19.220000000000002
150	21.65	31.075000000000003	28.675	18.6
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	0.5
17	0.5
18	0.5
19	1.5
20	2.0
21	5.5
22	10.0
23	13.0
24	17.5
25	19.5
26	26.5
27	47.0
28	70.5
29	70.5
30	67.5
31	71.5
32	83.0
33	105.5
34	119.0
35	120.5
36	133.0
37	137.5
38	149.0
39	203.5
40	233.5
41	238.5
42	216.0
43	182.0
44	150.0
45	130.0
46	138.0
47	150.0
48	158.0
49	110.5
50	81.5
51	91.0
52	83.0
53	85.0
54	62.0
55	29.5
56	22.0
57	27.5
58	37.0
59	33.5
60	28.0
61	17.5
62	8.5
63	13.5
64	15.5
65	13.5
66	11.5
67	18.0
68	23.5
69	20.0
70	20.0
71	22.5
72	18.5
73	10.0
74	8.0
75	6.0
76	3.0
77	2.0
78	2.0
79	1.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.497737556561084	33.425
2	21.266968325791854	23.5
3	8.280542986425338	13.725000000000001
4	4.615384615384616	10.2
5	2.0814479638009047	5.75
6	1.402714932126697	4.65
7	0.904977375565611	3.5000000000000004
8	0.3167420814479638	1.4000000000000001
9	0.18099547511312217	0.8999999999999999
>10	0.4524886877828055	2.9499999999999997
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	16	0.4	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	14	0.35000000000000003	No Hit
GCCGAGAGTGAGCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAAC	12	0.3	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	12	0.3	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	12	0.3	No Hit
GGAAAAGGCATAATATAAATTTTTAGAGTTACCAAATGTAGTGTGTATTC	11	0.27499999999999997	No Hit
TAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTC	11	0.27499999999999997	No Hit
GAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTC	10	0.25	No Hit
CAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAG	10	0.25	No Hit
AGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCA	10	0.25	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	9	0.22499999999999998	No Hit
AAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATA	9	0.22499999999999998	No Hit
GTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTT	9	0.22499999999999998	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	9	0.22499999999999998	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	8	0.2	No Hit
GGCAAATGTAAAAAGAAGAACTGCACTTATACACAGGTGCAAACTCGTAG	8	0.2	No Hit
CTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACT	8	0.2	No Hit
TTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCT	8	0.2	No Hit
GAAATTTGTAAACAACATACATCTCATGGGTTGGTCTTTGTTGTTCACCT	8	0.2	No Hit
GAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCC	8	0.2	No Hit
AGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGG	8	0.2	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	7	0.17500000000000002	No Hit
ATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACT	7	0.17500000000000002	No Hit
TACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACA	7	0.17500000000000002	No Hit
ATCCAGGTCTGTGGGAAACATTCAAAGTCATAAAGTTTAGATGACCAAGA	7	0.17500000000000002	No Hit
AGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCT	7	0.17500000000000002	No Hit
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	7	0.17500000000000002	No Hit
CTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGG	7	0.17500000000000002	No Hit
CAAAAAGTAATACTGCATTCTTCAATTAATGGCCCTTCTATTGGGAGACC	7	0.17500000000000002	No Hit
GTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGA	7	0.17500000000000002	No Hit
CTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACTCA	7	0.17500000000000002	No Hit
CAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATC	7	0.17500000000000002	No Hit
GCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCTT	7	0.17500000000000002	No Hit
AAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTA	7	0.17500000000000002	No Hit
GCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAG	7	0.17500000000000002	No Hit
CACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATCAAACA	7	0.17500000000000002	No Hit
CTCGTTTCCTTGCAAGTGAAATTTGTAAACAACATACATCTCATGGGTTG	7	0.17500000000000002	No Hit
AAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCT	7	0.17500000000000002	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	7	0.17500000000000002	No Hit
AATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTCTCCA	7	0.17500000000000002	No Hit
GCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAG	7	0.17500000000000002	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	6	0.15	No Hit
CTTTACTGTTCATATGGCAGCAAGAATTATCGCCAAGTTAGCTGCTTGGG	6	0.15	No Hit
CTCGTAGTGCTGATGAACCAATGACAACATTTGTTGTATGTAATGAATGT	6	0.15	No Hit
TTACATTATCCCTAATAATGGGGATAATGCTTTTGAGTGTCAAAAAGTAA	6	0.15	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	6	0.15	No Hit
GCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGA	6	0.15	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	6	0.15	No Hit
GTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCG	6	0.15	No Hit
GGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCA	6	0.15	No Hit
AATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCC	6	0.15	No Hit
CTATAATAGCTAAGAATCAGATGGTATTACATTATCCCTAATAATGGGGA	6	0.15	No Hit
GAGAAACTCGCCAAGAGTATGCTCTGGCTATGATTCAATGCAAAGTACTG	6	0.15	No Hit
TTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGACTC	6	0.15	No Hit
TAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGC	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
ACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAA	6	0.15	No Hit
GAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAA	6	0.15	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	6	0.15	No Hit
TTTCATCACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGC	6	0.15	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	6	0.15	No Hit
AGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAA	6	0.15	No Hit
CAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGT	6	0.15	No Hit
CAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCT	6	0.15	No Hit
CACCAGCGTGTCAGCATTTTCTTTGACTATGCGAAACGAAGCAAGAGCAC	6	0.15	No Hit
AGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAAAAC	6	0.15	No Hit
CTAAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTAT	6	0.15	No Hit
AAGAAATCAAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTA	6	0.15	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	6	0.15	No Hit
GCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTT	6	0.15	No Hit
CAGATGGTATTACATTATCCCTAATAATGGGGATAATGCTTTTGAGTGTC	6	0.15	No Hit
GTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATCTGGACCATTAAGAAAA	6	0.15	No Hit
CAAGAAGTGAAGAATGGCATTCCTTCTAACAGGATTATTTTGGGAGGATT	5	0.125	No Hit
AGCAGATGGAGTGAACTGTATAATGGGCGTTTTGAGTAACAAGTGTGGTT	5	0.125	No Hit
AAACCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAA	5	0.125	No Hit
CGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGAT	5	0.125	No Hit
CACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTAT	5	0.125	No Hit
GGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAA	5	0.125	No Hit
GAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAA	5	0.125	No Hit
GTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGTGTT	5	0.125	No Hit
GAGTGAGGCTCGCAACGTCTCCCACGGAGTCGCCTTCGTTCTGCTCTGGG	5	0.125	No Hit
ACTCAGCTGAGTTCACAGAAACTTCGTGGCAGCGGTGTTGCTGTTGAAAC	5	0.125	No Hit
AATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTC	5	0.125	No Hit
GATCCTTTTAAGTGTCTATGCATATATTTGTATTATACAATTCAGAATAT	5	0.125	No Hit
CTGTGATGCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTT	5	0.125	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	5	0.125	No Hit
GAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAA	5	0.125	No Hit
TCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATG	5	0.125	No Hit
GTTATAGACTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGT	5	0.125	No Hit
CTTCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGAT	5	0.125	No Hit
GAAACATTCAAAGTCATAAAGTTTAGATGACCAAGATGGACATTCGAGGT	5	0.125	No Hit
TAAGTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAATTC	5	0.125	No Hit
AAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAA	5	0.125	No Hit
CAATGACAACATTTGTTGTATGTAATGAATGTGGAAATCGGTGGAAGTTC	5	0.125	No Hit
AATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAA	5	0.125	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	5	0.125	No Hit
CTGAAACAGTTGGAGAATTTGGAGCAGCAGAAGTATGATGATGAAGATAT	5	0.125	No Hit
GTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAG	5	0.125	No Hit
CCGGTTTGCCTGGGTAGAAGCAGATGGAGTGAACTGTATAATGGGCGTTT	5	0.125	No Hit
CTCAGTATGTGCAGTGTGTTGCTGGCTGTCTGCAGCTGATGCTCCGGGTC	5	0.125	No Hit
GATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGAC	5	0.125	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	5	0.125	No Hit
GGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCG	5	0.125	No Hit
AGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCC	5	0.125	No Hit
GCTTTTGATAATCAGTCTCCTGTACAAATATTAAAATAATAATTTGATTT	5	0.125	No Hit
AAGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTG	5	0.125	No Hit
GAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACA	5	0.125	No Hit
GATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGAT	5	0.125	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	5	0.125	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	5	0.125	No Hit
CAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCA	5	0.125	No Hit
TCTGAGTGAGGCTCGCAACGTCTCCCACGGAGTCGCCTTCGTTCTGCTCT	5	0.125	No Hit
CAAATGTGTGAGCACCTTCGTCGCTATAATATCATTCCTGTTCTATCAGA	5	0.125	No Hit
GAAGATATCAGTGAAGACATCAAATTTCTTTTGGAAAAACTTGGTGAGAG	5	0.125	No Hit
GAAAAACTAATTTTGTAATTAGCTTTAAAATTAAGCCAGGCAACTCGTTT	5	0.125	No Hit
GAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAG	5	0.125	No Hit
CTAGCGAACAATACAGTCAGGATGGCTAAAGGTGACCCCAAGAAACCAAA	5	0.125	No Hit
AAAGAACTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.025	0.0	0.0	0.0	0.0
124-125	0.025	0.0	0.0	0.0	0.0
126-127	0.025	0.0	0.0	0.0	0.0
128-129	0.025	0.0	0.0	0.0	0.0
130-131	0.025	0.0	0.0	0.0	0.0
132-133	0.025	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
Read 1713505 spots for SRR32233447.sra
Written 1713505 spots for SRR32233447.sra
Read 1713500 spots for SRR32233447.sra
Written 1713500 spots for SRR32233447.sra
SRR ids: ['SRR32233447.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_66rl5mh4
SRR32233447.sra spots: 13708005
blocks: [[1, 1713500], [1713501, 3427000], [3427001, 5140500], [5140501, 6854000], [6854001, 8567500], [8567501, 10281000], [10281001, 11994500], [11994501, 13708005]]
SRR32233447 file size 4610112
SRR32233447 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233447 SRR32233447_1.fastq SRR32233447_2.fastq
Input file:	SRR32233447_1.fastq
Paired file:	SRR32233447_2.fastq
trimmed:	SRR32233447-trimmed-pair1.fastq, SRR32233447-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:27:19 2026 >> started

Fri Apr 17 04:27:35 2026 >> done (16.396s)
13708005 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
      10 ( 0.00%) empty read pairs filtered out after trimming by size control
13707995 (100.00%) read pairs available; of these:
  161890 ( 1.18%) trimmed read pairs available after processing
13546105 (98.82%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 24	       1	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       1	  0.00%
 64	       0	  0.00%
 65	       2	  0.00%
 66	       3	  0.00%
 67	       0	  0.00%
 68	       1	  0.00%
 69	       1	  0.00%
 70	       6	  0.00%
 71	       4	  0.00%
 72	       8	  0.00%
 73	      10	  0.00%
 74	       4	  0.00%
 75	      10	  0.00%
 76	       8	  0.00%
 77	       8	  0.00%
 78	      13	  0.00%
 79	       9	  0.00%
 80	       9	  0.00%
 81	      11	  0.00%
 82	      12	  0.00%
 83	      18	  0.00%
 84	      13	  0.00%
 85	      13	  0.00%
 86	      16	  0.00%
 87	      32	  0.00%
 88	      25	  0.00%
 89	      25	  0.00%
 90	      30	  0.00%
 91	      39	  0.00%
 92	      32	  0.00%
 93	      37	  0.00%
 94	      48	  0.00%
 95	      44	  0.00%
 96	      40	  0.00%
 97	      56	  0.00%
 98	      67	  0.00%
 99	      58	  0.00%
100	      59	  0.00%
101	      74	  0.00%
102	      77	  0.00%
103	      86	  0.00%
104	      71	  0.00%
105	      92	  0.00%
106	      87	  0.00%
107	      91	  0.00%
108	      97	  0.00%
109	     112	  0.00%
110	     119	  0.00%
111	     118	  0.00%
112	     100	  0.00%
113	     112	  0.00%
114	     124	  0.00%
115	     140	  0.00%
116	     133	  0.00%
117	     162	  0.00%
118	     176	  0.00%
119	     136	  0.00%
120	     157	  0.00%
121	     158	  0.00%
122	     169	  0.00%
123	     195	  0.00%
124	     191	  0.00%
125	     206	  0.00%
126	     203	  0.00%
127	     237	  0.00%
128	     228	  0.00%
129	     239	  0.00%
130	     255	  0.00%
131	     262	  0.00%
132	     274	  0.00%
133	     286	  0.00%
134	     287	  0.00%
135	     318	  0.00%
136	     297	  0.00%
137	     313	  0.00%
138	     337	  0.00%
139	     353	  0.00%
140	     366	  0.00%
141	   15347	  0.11%
142	   15644	  0.11%
143	   16231	  0.12%
144	   16229	  0.12%
145	   16969	  0.12%
146	   17659	  0.13%
147	   18219	  0.13%
148	   18535	  0.14%
149	   18846	  0.14%
150	13546105	 98.82%
13707995 reads passed initial QC


criterion=sequence-density
sequence-density=2.71
sequence-density-rank=1
fanout-score=2.78
fanout-score-rank=35
prefix-density=7.51
prefix-fanout=1.0
sequence=TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=56.67
fanout-score-rank=1
prefix-density=0.53
prefix-fanout=1.3
sequence=TTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTA


criterion=sequence-density
sequence-density=3.03
sequence-density-rank=1
fanout-score=4.99
fanout-score-rank=24
prefix-density=7.88
prefix-fanout=1.9
sequence=AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=24
fanout-score=125.89
fanout-score-rank=1
prefix-density=3.18
prefix-fanout=1.0
sequence=CTCCAATTTCAAAAGGACCATGCTTCGTATACATCCCTTCAGTACAATGGACCAGAAGAGAACATCTAGACAGTAGACTATAATAGCTTTTCTGAGGCTAGGCTGTCCTGGGGGCTGGTGTTAAGGGTATCTCAAAGGGTGGGTTGTTGCGACAAGGCCCAGACCCTAAGATGATAACTTTTTCCCACAGGTTCGAACCTCAGATATGGGTGGTTATGCCACATGTCATGACTTCACTGAAGCTGT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCTTGGAGGATGGTGAAGTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAGAATCCTGCAAAGAAAAATACTTCCGAGACGATGAATAGAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCTTGGTAGGTTCCTTCACGAATTACGTCTCGTCATCATTGATATATTGTGAGGATATTGGTGAGTAGGCCAAGGGTTAAT -y AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA -o SRR32233447 SRR32233447_1.fastq SRR32233447_2.fastq
Input file:	SRR32233447_1.fastq
Paired file:	SRR32233447_2.fastq
trimmed:	SRR32233447-trimmed-pair1.fastq, SRR32233447-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAGATA
-- paired 3' end adapter sequence (-y):	AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:28:51 2026 >> started

Fri Apr 17 04:29:08 2026 >> done (17.040s)
6853998 read pairs processed; of these:
    110 ( 0.00%) short read pairs filtered out after trimming by size control
   1033 ( 0.02%) empty read pairs filtered out after trimming by size control
6852855 (99.98%) read pairs available; of these:
     10 ( 0.00%) trimmed read pairs available after processing
6852845 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 24	      1	  0.00%
 25	      0	  0.00%
 26	      0	  0.00%
 27	      0	  0.00%
 28	      0	  0.00%
 29	      0	  0.00%
 30	      0	  0.00%
 31	      0	  0.00%
 32	      0	  0.00%
 33	      0	  0.00%
 34	      0	  0.00%
 35	      0	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      1	  0.00%
 64	      0	  0.00%
 65	      1	  0.00%
 66	      2	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      2	  0.00%
 71	      1	  0.00%
 72	      6	  0.00%
 73	      5	  0.00%
 74	      3	  0.00%
 75	      4	  0.00%
 76	      5	  0.00%
 77	      1	  0.00%
 78	      6	  0.00%
 79	      5	  0.00%
 80	      6	  0.00%
 81	      6	  0.00%
 82	      4	  0.00%
 83	     10	  0.00%
 84	     10	  0.00%
 85	      2	  0.00%
 86	      3	  0.00%
 87	     21	  0.00%
 88	     13	  0.00%
 89	      8	  0.00%
 90	     11	  0.00%
 91	     18	  0.00%
 92	     12	  0.00%
 93	     11	  0.00%
 94	     23	  0.00%
 95	     26	  0.00%
 96	     22	  0.00%
 97	     31	  0.00%
 98	     34	  0.00%
 99	     22	  0.00%
100	     26	  0.00%
101	     38	  0.00%
102	     30	  0.00%
103	     44	  0.00%
104	     31	  0.00%
105	     41	  0.00%
106	     41	  0.00%
107	     42	  0.00%
108	     48	  0.00%
109	     51	  0.00%
110	     64	  0.00%
111	     56	  0.00%
112	     41	  0.00%
113	     50	  0.00%
114	     63	  0.00%
115	     66	  0.00%
116	     52	  0.00%
117	     74	  0.00%
118	     69	  0.00%
119	     55	  0.00%
120	     78	  0.00%
121	     77	  0.00%
122	     80	  0.00%
123	     92	  0.00%
124	     93	  0.00%
125	    114	  0.00%
126	     91	  0.00%
127	    129	  0.00%
128	     99	  0.00%
129	    113	  0.00%
130	    128	  0.00%
131	    142	  0.00%
132	    139	  0.00%
133	    143	  0.00%
134	    150	  0.00%
135	    162	  0.00%
136	    146	  0.00%
137	    165	  0.00%
138	    182	  0.00%
139	    181	  0.00%
140	    199	  0.00%
141	   7665	  0.11%
142	   7596	  0.11%
143	   8099	  0.12%
144	   7900	  0.12%
145	   8226	  0.12%
146	   8619	  0.13%
147	   8950	  0.13%
148	   9096	  0.13%
149	   9651	  0.14%
150	6773032	 98.84%


criterion=sequence-density
sequence-density=1.34
sequence-density-rank=1
fanout-score=2.06
fanout-score-rank=36
prefix-density=1.35
prefix-fanout=2.0
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=37
fanout-score=104.92
fanout-score-rank=1
prefix-density=0.19
prefix-fanout=5.7
sequence=CAGATCCACTTTTATTAGGAACAAATACAATCTCAATCAGTACAAGTAGGCTTCAAGAGTTGATATTAATGGAAATCATCCAAATTACACTTGGGTCACAAATAATTACCCCACATAAAAAGGGAAAAAAAAATC


criterion=sequence-density
sequence-density=1.56
sequence-density-rank=1
fanout-score=2.32
fanout-score-rank=39
prefix-density=1.67
prefix-fanout=2.2
sequence=CCTAATCATATT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=48.72
fanout-score-rank=1
prefix-density=0.33
prefix-fanout=1.1
sequence=CCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTAATCCTTATCATAATTGCTCTCCCCTCTCTACGCATTCTATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTTCTGCCAATAGAACTTCCAATCCGTATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGAT
SRR32233447 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 04:30:05
                             Started mapping on |	Apr 17 04:30:05
                                    Finished on |	Apr 17 04:31:57
       Mapping speed, Million of reads per hour |	440.58

                          Number of input reads |	13706852
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11015533
                        Uniquely mapped reads % |	80.37%
                          Average mapped length |	298.98
                       Number of splices: Total |	9230348
            Number of splices: Annotated (sjdb) |	9163638
                       Number of splices: GT/AG |	9145103
                       Number of splices: GC/AG |	68559
                       Number of splices: AT/AC |	7839
               Number of splices: Non-canonical |	8847
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.67
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.34
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2550659
             % of reads mapped to multiple loci |	18.61%
        Number of reads mapped to too many loci |	11525
             % of reads mapped to too many loci |	0.08%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.92%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	140660	140660	140660
N_multimapping	2550659	2550659	2550659
N_noFeature	376618	10198675	790719
N_ambiguous	567509	42310	148904
UnstrandedReadsAssigned:10071406 PositiveStrandReadsAssigned:774548 NegativeStrandReadsAssigned:10075910
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233447 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233447-trimmed-pair1.fastq
                             SRR32233447-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,706,852 reads, 12,178,046 reads pseudoaligned
[quant] estimated average fragment length: 250.742
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,134 rounds

 109283 SRR32233447.ke.tsv
  52636 SRR32233447.se.tsv
 161919 total
==> SRR32233447.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	5.66667	0	0
ENSMUST00000196221.1	9	6	0	0
ENSMUST00000179664.1	11	8	0	0
ENSMUST00000178537.1	12	9	0	0
ENSMUST00000178862.1	14	6	0	0
ENSMUST00000179520.1	11	8	0	0
ENSMUST00000179883.1	16	5.66667	0	0
ENSMUST00000195858.1	10	7	0	0
ENSMUST00000179932.1	12	9	0	0

==> SRR32233447.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233447 completed mapping pipeline successfully
