Starting /dee2/code/volunteer_pipeline.sh SRR32233440
    current disk space = 848023428
    free memory = 195422928 
SRR32233440 SRAfilesize
ae77305392c1d64da59743918a2a1001  SRR32233440.sra
SRR32233440.sra file validated
SRR32233440 is paired end
SRR32233440 is conventional basespace
SRR32233440 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.5365	37.0	37.0	37.0	37.0	37.0
2	36.54175	37.0	37.0	37.0	37.0	37.0
3	36.646	37.0	37.0	37.0	37.0	37.0
4	36.6575	37.0	37.0	37.0	37.0	37.0
5	36.7125	37.0	37.0	37.0	37.0	37.0
6	36.6365	37.0	37.0	37.0	37.0	37.0
7	36.577	37.0	37.0	37.0	37.0	37.0
8	36.6025	37.0	37.0	37.0	37.0	37.0
9	36.676	37.0	37.0	37.0	37.0	37.0
10-14	36.63165	37.0	37.0	37.0	37.0	37.0
15-19	36.5951	37.0	37.0	37.0	37.0	37.0
20-24	36.6165	37.0	37.0	37.0	37.0	37.0
25-29	36.6007	37.0	37.0	37.0	37.0	37.0
30-34	36.5586	37.0	37.0	37.0	37.0	37.0
35-39	36.5147	37.0	37.0	37.0	37.0	37.0
40-44	36.502199999999995	37.0	37.0	37.0	37.0	37.0
45-49	36.4953	37.0	37.0	37.0	37.0	37.0
50-54	36.4829	37.0	37.0	37.0	37.0	37.0
55-59	36.4385	37.0	37.0	37.0	37.0	37.0
60-64	36.366	37.0	37.0	37.0	37.0	37.0
65-69	36.360499999999995	37.0	37.0	37.0	37.0	37.0
70-74	36.2787	37.0	37.0	37.0	37.0	37.0
75-79	36.2909	37.0	37.0	37.0	37.0	37.0
80-84	36.186	37.0	37.0	37.0	37.0	37.0
85-89	36.16975000000001	37.0	37.0	37.0	37.0	37.0
90-94	36.0621	37.0	37.0	37.0	37.0	37.0
95-99	36.088550000000005	37.0	37.0	37.0	37.0	37.0
100-104	35.9785	37.0	37.0	37.0	37.0	37.0
105-109	35.901799999999994	37.0	37.0	37.0	37.0	37.0
110-114	35.834	37.0	37.0	37.0	37.0	37.0
115-119	35.792500000000004	37.0	37.0	37.0	37.0	37.0
120-124	35.7258	37.0	37.0	37.0	37.0	37.0
125-129	35.5108	37.0	37.0	37.0	37.0	37.0
130-134	35.409800000000004	37.0	37.0	37.0	32.2	37.0
135-139	35.3069	37.0	37.0	37.0	29.8	37.0
140-144	35.2133	37.0	37.0	37.0	25.0	37.0
145-149	35.0233	37.0	37.0	37.0	25.0	37.0
150	34.728	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	4.0
27	7.0
28	8.0
29	18.0
30	22.0
31	35.0
32	36.0
33	73.0
34	132.0
35	535.0
36	3037.0
37	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1	8.575000000000001	11.600000000000001	36.725
2	20.90112640801001	10.012515644555695	35.04380475594493	34.04255319148936
3	22.686343171585793	12.906453226613307	27.56378189094547	36.84342171085543
4	27.150000000000002	17.625	26.700000000000003	28.525
5	27.875	20.9	26.5	24.725
6	28.075	25.3	29.099999999999998	17.525
7	19.400000000000002	23.325000000000003	39.125	18.15
8	17.9	25.924999999999997	33.95	22.225
9	16.8	27.750000000000004	35.85	19.6
10-14	19.93599679983999	27.591379568978446	30.391519575978798	22.08110405520276
15-19	21.740000000000002	27.689999999999998	29.104999999999997	21.465
20-24	19.855	28.720000000000002	28.96	22.465
25-29	20.119999999999997	26.96	29.025000000000002	23.895
30-34	20.7	28.025	26.955000000000002	24.32
35-39	21.117111711171116	27.45274527452745	28.477847784778476	22.95229522952295
40-44	22.655	27.595	26.815	22.935
45-49	21.884999999999998	24.86	29.310000000000002	23.945
50-54	21.0	28.305000000000003	27.439999999999998	23.255
55-59	19.015	27.415	30.669999999999998	22.900000000000002
60-64	21.834999999999997	27.029999999999998	28.255000000000003	22.88
65-69	21.08	26.534999999999997	29.085	23.3
70-74	20.28	27.834999999999997	28.895	22.99
75-79	21.425	26.424999999999997	29.770000000000003	22.38
80-84	20.405	26.790000000000003	30.135	22.67
85-89	19.470973548677435	26.696334816740837	29.906495324766237	23.926196309815488
90-94	22.35	25.965	29.535	22.15
95-99	20.836041802090104	25.246262313115658	30.63653182659133	23.28116405820291
100-104	21.785	26.39	28.689999999999998	23.135
105-109	21.48	25.515	29.115000000000002	23.89
110-114	22.892289228922895	26.237623762376238	27.402740274027405	23.467346734673466
115-119	20.79	26.465	29.659999999999997	23.085
120-124	23.067306730673067	23.74737473747375	29.55795579557956	23.62736273627363
125-129	20.79	26.305	31.25	21.654999999999998
130-134	20.925	28.535	29.285	21.255
135-139	21.22	27.965	28.384999999999998	22.43
140-144	20.9	27.245	28.79	23.064999999999998
145-149	21.545	26.56	27.74	24.154999999999998
150	21.55	28.249999999999996	27.55	22.650000000000002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	1.0
22	1.0
23	0.5
24	0.5
25	4.5
26	11.0
27	19.5
28	24.5
29	24.5
30	32.0
31	36.5
32	33.0
33	34.0
34	49.0
35	63.5
36	91.0
37	116.5
38	130.5
39	206.0
40	249.5
41	229.5
42	231.0
43	259.0
44	235.0
45	164.5
46	156.5
47	219.5
48	221.5
49	154.5
50	131.5
51	131.0
52	129.0
53	127.5
54	93.5
55	50.0
56	57.5
57	45.0
58	19.0
59	30.5
60	44.0
61	31.0
62	11.0
63	8.5
64	7.5
65	3.5
66	3.5
67	7.0
68	13.0
69	11.0
70	6.5
71	6.0
72	7.0
73	7.0
74	5.0
75	3.5
76	4.0
77	3.5
78	0.0
79	0.5
80	1.0
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.125
3	0.05
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.005
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.01
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.005
90-94	0.0
95-99	0.005
100-104	0.0
105-109	0.0
110-114	0.01
115-119	0.0
120-124	0.01
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.90169689877121	22.175
2	23.756582796957286	20.3
3	10.53247513165594	13.5
4	4.154476301930954	7.1
5	3.0427150380339376	6.5
6	1.638385020479813	4.2
7	1.0532475131655938	3.15
8	1.2287887653598597	4.2
9	0.29256875365710944	1.125
>10	2.399063779988297	17.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCTGGTCTTCATGG	40	1.0	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	38	0.95	No Hit
GTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCC	35	0.8750000000000001	No Hit
CTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAGGT	34	0.8500000000000001	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	28	0.7000000000000001	No Hit
GCTTCGTTTCGCATAGTCAAAGAAAATGCTGACACGCTGGTGATTTTCCT	26	0.65	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	26	0.65	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	24	0.6	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	24	0.6	No Hit
GTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCT	24	0.6	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	21	0.525	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	21	0.525	No Hit
CTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATTAG	20	0.5	No Hit
GCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCC	19	0.475	No Hit
GCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACT	18	0.44999999999999996	No Hit
GGTATGTATTTGCAAAACATTTTAACACTGCACACATTAGAAGTTCTATG	16	0.4	No Hit
GCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGC	16	0.4	No Hit
GTCCTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCAC	14	0.35000000000000003	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	14	0.35000000000000003	No Hit
CCATGGACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTT	14	0.35000000000000003	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	14	0.35000000000000003	No Hit
GACCAATCTTTTTTTTGGGGGGGGGAGGGGGGTTGAGACAGGGTTTCTCT	13	0.325	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	12	0.3	No Hit
CAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCC	12	0.3	No Hit
CCCAGTTCTTCTTCATCAGCTCCAATTGCAACATAATCATCTCCTGTCCG	12	0.3	No Hit
CTAGGCTATAAAAAGTTAATATTTAATAAAATGCAACATCAACTTATGAA	12	0.3	No Hit
CCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATCAT	12	0.3	No Hit
CCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTATC	12	0.3	No Hit
CTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAAC	11	0.27499999999999997	No Hit
CTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGAA	11	0.27499999999999997	No Hit
GGTGAGAGCAGTGTATAAAGACAAGGCGCCTCCCTGAGAAAATCCTCCCA	11	0.27499999999999997	No Hit
GCTTCCTTTAGTGCAAGTTCAACCTCTAACTTACATCTCATGTCTGACAA	11	0.27499999999999997	No Hit
CTCAGGCTTAATCCTTGGTCAGTAGGTTTCCAAGTGCAAGTACAGTCATG	11	0.27499999999999997	No Hit
CCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTACTC	11	0.27499999999999997	No Hit
AGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGT	11	0.27499999999999997	No Hit
CGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAG	11	0.27499999999999997	No Hit
CCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGC	11	0.27499999999999997	No Hit
GGCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	10	0.25	No Hit
CTTCACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAAC	10	0.25	No Hit
CCCGGGGATAATGCCGGACATATTCTCCAACATCGTGAGCAGCAACAGCT	10	0.25	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	10	0.25	No Hit
GTCTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACA	9	0.22499999999999998	No Hit
GCCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTC	9	0.22499999999999998	No Hit
CTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTC	9	0.22499999999999998	No Hit
GTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACTGG	9	0.22499999999999998	No Hit
GTCCCACCAGTCTTGGCCATCTGATGCTCCCTGATGGCTTCTTTGGTCAG	9	0.22499999999999998	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	8	0.2	No Hit
GCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAAC	8	0.2	No Hit
GCACCACCACGCCCGGCTATGTCTGTATCTCAAAATGTCAAAATAAAATA	8	0.2	No Hit
CCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAG	8	0.2	No Hit
CCGGTACTCATTGACCCGGAGCATCAGCTGCAGACAGCCAGCAACACACT	8	0.2	No Hit
ATGAGATGTATGTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCT	8	0.2	No Hit
CTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTTCTCCG	8	0.2	No Hit
CCTGGACACTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCACTG	8	0.2	No Hit
CCTGGCTTAATTTTAAAGCTAATTACAAAATTAGTTTTTCTTAATGGTCC	8	0.2	No Hit
CGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAG	8	0.2	No Hit
CCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTAT	8	0.2	No Hit
ACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGG	8	0.2	No Hit
CGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGAA	8	0.2	No Hit
GTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCT	8	0.2	No Hit
GGTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCA	8	0.2	No Hit
GTCGGGGTAGTTGCAATAGTAGACCGCTGAGCTAGCGTCGGACACCACGG	8	0.2	No Hit
GTGGGGAGCAAGTCCCTCAAGGCATTTAAAACAAAAATCTCGTGTAGCCC	8	0.2	No Hit
GGCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGT	8	0.2	No Hit
CTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAACTGTTT	8	0.2	No Hit
GTCTCCCAGCTGATCTGAGACCACAGAAGGCATTTAAGACAGACTGTCAG	8	0.2	No Hit
GTTGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCC	8	0.2	No Hit
CCCCTTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGA	7	0.17500000000000002	No Hit
GAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAGC	7	0.17500000000000002	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	7	0.17500000000000002	No Hit
CTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTCT	7	0.17500000000000002	No Hit
CTGGTGATTTTCCTGCAGCATATCGTCAACCATGGTTAGTATGTACTGCA	7	0.17500000000000002	No Hit
CTCCTGAATTAAGTGTTCTTTGTCTTGCTCGTGGTTCTTGACCAGTCTCT	7	0.17500000000000002	No Hit
GCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGG	7	0.17500000000000002	No Hit
CTCCCAGGAATTACCATAGGGAAAAGCTATACAACAGAAAAGAAGTAATC	7	0.17500000000000002	No Hit
GTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCT	7	0.17500000000000002	No Hit
ACACGCTCATACACACACACACACTCACTTATACACACACACACTCACAC	7	0.17500000000000002	No Hit
CGCCCATTATACAGTTCACTCCATCTGCTTCTACCCAGGCAAACCGGTAC	7	0.17500000000000002	No Hit
GTCCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGA	7	0.17500000000000002	No Hit
CTTCTGCACGGCCAGAAGAGCATTGTAGCGAACCTGCTGGTCTTCATGGT	7	0.17500000000000002	No Hit
GCTCCTGAATTAAGTGTTCTTTGTCTTGCTCGTGGTTCTTGACCAGTCTC	7	0.17500000000000002	No Hit
TTTAAGTGTTTTACAGAAAAATAAAACCACAGATTTATTACCAGGACTGG	7	0.17500000000000002	No Hit
CTCAACTGTTCAAGTGGCAGACAAAATAAATTACCATAAATTATATGCCA	7	0.17500000000000002	No Hit
CATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAGCAG	7	0.17500000000000002	No Hit
GCCAGGAGCCATATTGAAAAAATCATTTGGTATTGAAGTTGAAAACCACA	7	0.17500000000000002	No Hit
CACTGATATCTTCATCATCATACTTCTGCTGCTCCAAATTCTCCAACTGT	6	0.15	No Hit
CTCCAGAAGTTTTGTCAAGATTTTCAAGAGTTCATAGTTCTTCTCATTCA	6	0.15	No Hit
CCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGCACCACCAC	6	0.15	No Hit
CTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATT	6	0.15	No Hit
GCCACTTCATGTAAACCACTGACACTTTGAGAAACTACAATATGATCGTG	6	0.15	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	6	0.15	No Hit
ACCTCCAGAAGTTTTGTCAAGATTTTCAAGAGTTCATAGTTCTTCTCATT	6	0.15	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	6	0.15	No Hit
GATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCGTTTC	6	0.15	No Hit
GCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGT	6	0.15	No Hit
AGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTT	6	0.15	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	6	0.15	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	6	0.15	No Hit
GGAGCTTATCAATGAAGTGCTTGACATCCATCATTTCCTGCTGACATGAG	6	0.15	No Hit
GCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCATT	6	0.15	No Hit
CTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAAACC	6	0.15	No Hit
GGGGAACTAAAGGGTCACAATCTCCATGGCACTGGAGAACGGAAATATCT	6	0.15	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	6	0.15	No Hit
CTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTC	6	0.15	No Hit
TTCGGTTTGTTTGTTTGTTTGTTTCCGAGACAGGGTTTCTCTGTGTAGCC	6	0.15	No Hit
TCTGGGATTAAAGGTGTGGGCCACCACGCCCGGCAGACCAATCCTTTTAA	6	0.15	No Hit
GCCCCCTCCAAAAGGCATCTTCCCCTCACAGCCTCCACGCCAAACAAGGA	6	0.15	No Hit
GTCCAGATACTTTGCCAATTCTTCCAAACTCAACAGAACTTCCACCGATT	6	0.15	No Hit
GCATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCAAACCTTTGAATA	6	0.15	No Hit
GCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCATA	6	0.15	No Hit
GGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCT	6	0.15	No Hit
CCTGGAACTCACTCTGTGGACCAGGCTATTGACAGAGATCCGCCTGCCTC	6	0.15	No Hit
GTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTCCAACGATA	6	0.15	No Hit
CCTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCC	5	0.125	No Hit
TGCTGGTAACAGACCCAAAGCTTTAATACCCACAAGAATCCATGTGCAGC	5	0.125	No Hit
GTGCTCACACATTTGAGGACTGAATGCCAGGAGCCATATTGAAAAAATCA	5	0.125	No Hit
TCAGGGTCTTTATCAGTTGATGGTCCATCTAATAATTTTTTCCAGGATTT	5	0.125	No Hit
GGCAGTATCTAATTTTATACAATTTAACAAGCAAACACTTTTATTCTCCC	5	0.125	No Hit
CCTCCACCCGCTTCATGCGCTTCACCTGCTTGCGCCGCCGCGGCCGGTAC	5	0.125	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	5	0.125	No Hit
CTCTCACCAAGTTTTTCCAAAAGAAATTTGATGTCTTCACTGATATCTTC	5	0.125	No Hit
CTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCCATTCCAATCTTCCA	5	0.125	No Hit
GGGGACTTTTGATACCTGCAAAGGCTTCTGCCCATCCATGCCCTGTATCT	5	0.125	No Hit
CGCCTACTATCTATTGCATTGATGACTGAAGAGTAAAGTGACTCCACCAT	5	0.125	No Hit
GTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTT	5	0.125	No Hit
GGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCAAG	5	0.125	No Hit
TCCAACGATATCAAACCAAGAAGGCATAGCCATATTCATATTTAATGTGA	5	0.125	No Hit
CTGTTCTTTGGAGATGTGCGTCATCAGATTTATAAATGTTTTAGCACACT	5	0.125	No Hit
GTTGGGACAGCAGCATCCACAGCACCTCGAATGTCCATCTTGGTCATCTA	5	0.125	No Hit
GGTCCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCA	5	0.125	No Hit
GCCCCTGTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTG	5	0.125	No Hit
CTGACTGTAGTTGTTTACCAAGATATTCCCAGTTGTGCACCATGAGCTTC	5	0.125	No Hit
TTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCTT	5	0.125	No Hit
CCATGGGAAGTGCAGAAAGGCTGAACAGGTCAGCTCAGCTCCGAGCAGCA	5	0.125	No Hit
GATGAGTTGGTACCTAAACCCAATCCAGACAAGATATGTTGTGCTATGTT	5	0.125	No Hit
TTCAGTTTGAAGCATCTCTTGCTTGTCCTCGGAACTTCTTTTCATTTCAA	5	0.125	No Hit
CTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCTCATCACTAGCCATT	5	0.125	No Hit
CACGGGCTTCAAAACATGCCTTCCTACAATGCTTATCCCAGCAACCACTC	5	0.125	No Hit
CTGTTCTTAAGTTTTCGGTCTCTTGTTCAAGTAAAGACTTCAGCATTTCC	5	0.125	No Hit
TGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTGAAAGTC	5	0.125	No Hit
CTCCACGCCAAACAAGGAGCATCAAGAATCTGTCTGGGTTGTTTCATTCT	5	0.125	No Hit
ATTCCCAGTTGTGCACCATGAGCTTCTGCACGGCCAGAAGAGCATTGTAG	5	0.125	No Hit
GTTTGTTTGAACTTTATTTTTATTTTGTTTTTTAGTCTATGTGTATGTGT	5	0.125	No Hit
GTTGTTTACAAATTTCACTTGCAAGGAAACGAGTTGCCTGGCTTAATTTT	5	0.125	No Hit
GTCCCTGGTAGGGAAGACCCATCTCGGGCTTATACACAAAGGGCAGATAC	5	0.125	No Hit
TGGCGATTCAACATTGGCAGGAAGTATGGCCAGGCAGTGCTCTTGCTTCG	5	0.125	No Hit
GCTGGGATTAAAGGTGTGCGCCACCACCATCTGGCTGATTCCATGTCTTC	5	0.125	No Hit
CTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGA	5	0.125	No Hit
GGCCTTTACTATATGCATTACAACATCTGTGTCAGGAAGACGATGTGAAA	5	0.125	No Hit
TCCCCCAAGCAGCTAACTTGGCGATAATTCTTGCTGCCATATGAACAGTA	5	0.125	No Hit
GCCAATTCTTCCAAACTCAACAGAACTTCCACCGATTTCCACATTCATTA	5	0.125	No Hit
TCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGT	5	0.125	No Hit
TCTCCCTACATTTTAATCGCACAGAATCAGAAGTGCTTGGTGCCCGAGGA	5	0.125	No Hit
CCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGC	5	0.125	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	5	0.125	No Hit
CCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGG	5	0.125	No Hit
GTGCAGTTATTTGAAATATATAAACTTAAGTAATGTTTGATTTCTTTTCA	5	0.125	No Hit
GTCTGCTTTGGTATGTAAAATATGCACTTCAGCTCCTTGTCCTGGGGTCT	5	0.125	No Hit
TGGGACTATTTTAACCTTATTACTTTTAAACTAATTCTTTCAAACTGTAC	5	0.125	No Hit
GCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTG	5	0.125	No Hit
TGGAAATACTGTATGTCTGTGTTTCGGTTTGTTTGTTTGTTTGTTTCCGA	5	0.125	No Hit
CCTACATTTCAGCTGGCTTGACATCATCATGTAAGAAAAATTTCAGCACA	5	0.125	No Hit
CCGTTTTCAGAGTAAAATCTATTAAAAAATGGTATTATATATGTATGAGA	5	0.125	No Hit
GTGGTTCATCACCAACTGCTTTCCACCAAGCTGCTCAATAACCCGTTTTC	5	0.125	No Hit
CTCACGGCATTTTCTCTCCAAAATTTCTCAGACTTGTGTACAGGGCTCCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.025	0.0	0.0	0.0	0.0
136-137	0.025	0.0	0.0	0.0	0.0
138	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCTG	20	0.006139246	28.8	60-64
AGGAGCA	20	0.006139246	28.8	45-49
>>END_MODULE
SRR32233440 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32233440_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.241	37.0	37.0	37.0	37.0	37.0
2	36.5605	37.0	37.0	37.0	37.0	37.0
3	36.718	37.0	37.0	37.0	37.0	37.0
4	36.638	37.0	37.0	37.0	37.0	37.0
5	36.6075	37.0	37.0	37.0	37.0	37.0
6	36.5815	37.0	37.0	37.0	37.0	37.0
7	36.5585	37.0	37.0	37.0	37.0	37.0
8	36.60425	37.0	37.0	37.0	37.0	37.0
9	36.659	37.0	37.0	37.0	37.0	37.0
10-14	36.568999999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.5431	37.0	37.0	37.0	37.0	37.0
20-24	36.5641	37.0	37.0	37.0	37.0	37.0
25-29	36.5004	37.0	37.0	37.0	37.0	37.0
30-34	36.47025	37.0	37.0	37.0	37.0	37.0
35-39	36.438100000000006	37.0	37.0	37.0	37.0	37.0
40-44	36.41415	37.0	37.0	37.0	37.0	37.0
45-49	36.33145	37.0	37.0	37.0	37.0	37.0
50-54	36.308099999999996	37.0	37.0	37.0	37.0	37.0
55-59	36.27515	37.0	37.0	37.0	37.0	37.0
60-64	36.17875	37.0	37.0	37.0	37.0	37.0
65-69	36.1197	37.0	37.0	37.0	37.0	37.0
70-74	36.067899999999995	37.0	37.0	37.0	37.0	37.0
75-79	35.97055	37.0	37.0	37.0	37.0	37.0
80-84	35.9188	37.0	37.0	37.0	37.0	37.0
85-89	35.8018	37.0	37.0	37.0	37.0	37.0
90-94	35.67815	37.0	37.0	37.0	37.0	37.0
95-99	35.54645	37.0	37.0	37.0	37.0	37.0
100-104	35.42190000000001	37.0	37.0	37.0	34.6	37.0
105-109	35.358450000000005	37.0	37.0	37.0	32.2	37.0
110-114	35.372299999999996	37.0	37.0	37.0	32.2	37.0
115-119	35.126200000000004	37.0	37.0	37.0	25.0	37.0
120-124	34.9779	37.0	37.0	37.0	25.0	37.0
125-129	34.7841	37.0	37.0	37.0	25.0	37.0
130-134	34.653800000000004	37.0	37.0	37.0	25.0	37.0
135-139	34.414	37.0	37.0	37.0	25.0	37.0
140-144	34.3596	37.0	37.0	37.0	25.0	37.0
145-149	34.220099999999995	37.0	37.0	37.0	25.0	37.0
150	34.186	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	0.0
22	0.0
23	0.0
24	3.0
25	5.0
26	4.0
27	9.0
28	5.0
29	10.0
30	20.0
31	37.0
32	39.0
33	114.0
34	295.0
35	1145.0
36	2274.0
37	37.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.74187093546773	23.686843421710854	12.906453226613307	29.664832416208103
2	21.75	33.475	30.475	14.299999999999999
3	24.55	32.525	21.65	21.275
4	24.95	37.35	19.225	18.475
5	24.325	33.35	23.575	18.75
6	20.925	37.625	23.75	17.7
7	23.0	20.375	35.925000000000004	20.7
8	21.380345086271568	22.48062015503876	28.732183045761438	27.406851712928233
9	23.849999999999998	25.825	28.549999999999997	21.775
10-14	24.935	28.444999999999997	24.01	22.61
15-19	23.32	28.875	26.619999999999997	21.185000000000002
20-24	23.51	28.655	26.0	21.834999999999997
25-29	24.169999999999998	29.15	26.195	20.485
30-34	23.51117555877794	27.546377318865943	27.156357817890896	21.78608930446522
35-39	23.715	28.54	27.045	20.7
40-44	23.85619280964048	28.23141157057853	26.066303315165758	21.84609230461523
45-49	23.151157557877895	28.576428821441073	26.151307565378268	22.121106055302764
50-54	22.845	29.549999999999997	25.480000000000004	22.125
55-59	22.57612880644032	29.386469323466173	26.916345817290864	21.12105605280264
60-64	22.573386007901185	28.809321398209732	26.403960594089114	22.21333199979997
65-69	23.84738473847385	28.64786478647865	26.5026502650265	21.002100210021002
70-74	22.835	28.46	26.93	21.775
75-79	23.231161558077904	28.59142957147857	26.741337066853344	21.436071803590178
80-84	23.075000000000003	30.365	25.685000000000002	20.875
85-89	23.467346734673466	29.15291529152915	26.1976197619762	21.182118211821184
90-94	22.55112755637782	29.406470323516174	25.946297314865742	22.096104805240262
95-99	23.891194559727985	28.281414070703537	27.44637231861593	20.38101905095255
100-104	23.62736273627363	28.862886288628864	26.017601760176017	21.49214921492149
105-109	23.821191059552977	28.766438321916095	25.99629981499075	21.416070803540176
110-114	22.402240224022403	29.167916791679165	26.772677267726774	21.657165716571658
115-119	22.642264226422643	28.032803280328032	27.282728272827285	22.042204220422043
120-124	22.875	29.104999999999997	27.139999999999997	20.880000000000003
125-129	23.452345234523452	28.722872287228725	26.467646764676466	21.357135713571356
130-134	23.05230523052305	29.957995799579955	25.532553255325535	21.457145714571457
135-139	23.87	28.87	26.25	21.01
140-144	23.665	29.970000000000002	25.855	20.51
145-149	23.405	29.134999999999998	26.705000000000002	20.755000000000003
150	24.275	29.799999999999997	25.8	20.125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	1.0
24	3.0
25	4.0
26	7.0
27	18.5
28	17.5
29	13.5
30	19.0
31	24.5
32	35.5
33	64.0
34	87.0
35	76.5
36	91.0
37	131.5
38	162.5
39	219.5
40	251.0
41	250.0
42	242.0
43	206.0
44	196.0
45	183.0
46	164.0
47	185.0
48	192.0
49	141.0
50	97.5
51	96.5
52	112.0
53	127.5
54	95.0
55	49.0
56	40.0
57	36.5
58	27.0
59	27.0
60	25.5
61	16.0
62	9.0
63	9.5
64	16.0
65	16.5
66	19.5
67	29.5
68	27.0
69	13.0
70	11.5
71	31.0
72	38.0
73	20.0
74	5.5
75	4.0
76	4.0
77	2.5
78	3.5
79	2.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.025
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.005
35-39	0.0
40-44	0.005
45-49	0.005
50-54	0.0
55-59	0.005
60-64	0.015
65-69	0.01
70-74	0.0
75-79	0.005
80-84	0.0
85-89	0.01
90-94	0.005
95-99	0.005
100-104	0.01
105-109	0.005
110-114	0.01
115-119	0.01
120-124	0.0
125-129	0.01
130-134	0.01
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.275000000000006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.41345685031692	26.875
2	26.036079960994634	26.700000000000003
3	11.165285226718675	17.175
4	3.9492930277913216	8.1
5	2.9741589468551926	7.625
6	0.9751340809361287	3.0
7	0.9263773768893223	3.325
8	0.43881033642125794	1.7999999999999998
9	0.8288639687957094	3.8249999999999997
>10	0.29254022428083865	1.575
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAG	11	0.27499999999999997	No Hit
CAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGA	11	0.27499999999999997	No Hit
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	11	0.27499999999999997	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	10	0.25	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	10	0.25	No Hit
CAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCA	10	0.25	No Hit
CTCGCCAAGAGTATGCTCTGGCTATGATTCAATGCAAAGTACTGAAACAG	9	0.22499999999999998	No Hit
GAAAAATCAACGGAAAGAGAAACTCGCCAAGAGTATGCTCTGGCTATGAT	9	0.22499999999999998	No Hit
AGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGG	9	0.22499999999999998	No Hit
CTGATGGAAGGCAGTGACTTAAATTACTATTTCAATTGGATAAAAACTCA	9	0.22499999999999998	No Hit
AAAAGAAGAACTGCACTTATACACAGGTGCAAACTCGTAGTGCTGATGAA	9	0.22499999999999998	No Hit
GAAGAACTATGAACTCTTGAAAATCTTGACAAAACTTCTGGAGGTATCAG	9	0.22499999999999998	No Hit
CTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTA	9	0.22499999999999998	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	9	0.22499999999999998	No Hit
CAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCA	9	0.22499999999999998	No Hit
CGGGCCGGGTCTGTGAGGAAGGCCTAGGCCAGCGGCTTCGCGGCTTGTCC	9	0.22499999999999998	No Hit
GAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGAA	9	0.22499999999999998	No Hit
GGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCCT	9	0.22499999999999998	No Hit
GGCGTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAA	9	0.22499999999999998	No Hit
GCAGAAGTATGATGATGAAGATATCAGTGAAGACATCAAATTTCTTTTGG	9	0.22499999999999998	No Hit
TGGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTG	9	0.22499999999999998	No Hit
AGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTC	9	0.22499999999999998	No Hit
CGAAAGATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAG	9	0.22499999999999998	No Hit
GAGTGAGGCTCGCAACGTCTCCCACGGAGTCGCCTTCGTTCTGCTCTGGG	8	0.2	No Hit
TGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGGACCTGCTGCGGTCC	8	0.2	No Hit
CTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACA	8	0.2	No Hit
CTAATGTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCC	8	0.2	No Hit
AATACAGTCAGGATGGCTAAAGGTGACCCCAAGAAACCAAAGGGCAAGAT	8	0.2	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	8	0.2	No Hit
AGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATG	8	0.2	No Hit
GAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCC	8	0.2	No Hit
ATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGATACAGACATA	8	0.2	No Hit
TAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATT	7	0.17500000000000002	No Hit
GTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGGAAAC	7	0.17500000000000002	No Hit
ATTTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACT	7	0.17500000000000002	No Hit
CAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCT	7	0.17500000000000002	No Hit
GGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCA	7	0.17500000000000002	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	7	0.17500000000000002	No Hit
ATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCC	7	0.17500000000000002	No Hit
AATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAAT	7	0.17500000000000002	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	7	0.17500000000000002	No Hit
TGGAAATCGGTGGAAGTTCTGTTGAGTTTGGAAGAATTGGCAAAGTATCT	7	0.17500000000000002	No Hit
GAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAA	7	0.17500000000000002	No Hit
TGAGCAGCTTGGTGGAAAGCAGTTGGTGATGAACCACATGCACCATGAAG	7	0.17500000000000002	No Hit
AGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTG	7	0.17500000000000002	No Hit
GTGATAGTTCTCAGTATGTGCAGTGTGTTGCTGGCTGTCTGCAGCTGATG	7	0.17500000000000002	No Hit
CAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTG	7	0.17500000000000002	No Hit
GAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGC	7	0.17500000000000002	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	7	0.17500000000000002	No Hit
TTCTTGAGCCCTCCTTTAAGAAGCAATTTTTAAGGGAGAGTAATAGTTGA	7	0.17500000000000002	No Hit
TAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTC	7	0.17500000000000002	No Hit
AAAAGAACCAAGAAAGAAGAGGACCTTGGTCCAGAAGAAGTCACAGCGTC	6	0.15	No Hit
GAAGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACT	6	0.15	No Hit
GGAATATCTTGGTAAACAACTACAGTCAGAGCAGCCTCAGACTGCTGCTG	6	0.15	No Hit
GGCTGTTCCAGACCCTTGGTTCTGAAACCTCTTTTTGCTCACTTAGGGAT	6	0.15	No Hit
GAAGCTCATGGTGCACAACTGGGAATATCTTGGTAAACAACTACAGTCAG	6	0.15	No Hit
CAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTG	6	0.15	No Hit
GCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTA	6	0.15	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	6	0.15	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	6	0.15	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	6	0.15	No Hit
GGAGGTTGCAAAAAAAGGCACCTCCTTTAGACTGAATCTTTTGTGCTGAA	6	0.15	No Hit
CCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAATC	6	0.15	No Hit
GTTCTATCAGACATCCTTCAAGAATCTGTCAAAGAGAAAGTAACAAGAAT	6	0.15	No Hit
ATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAA	6	0.15	No Hit
CACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTA	6	0.15	No Hit
GCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCAT	6	0.15	No Hit
AAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAG	6	0.15	No Hit
AGATGATCCACAAGTCTTAGCTGTTGCTGCTCACGATGTTGGAGAATATG	6	0.15	No Hit
CAGGTCTGTGGGAAACATTCAAAGTCATAAAGTTTAGATGACCAAGATGG	6	0.15	No Hit
GTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGGCTATG	6	0.15	No Hit
GTTTTCACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCT	5	0.125	No Hit
CTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGACATAGT	5	0.125	No Hit
ATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTA	5	0.125	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	5	0.125	No Hit
TTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAA	5	0.125	No Hit
GAACTATGAACTCTTGAAAATCTTGACAAAACTTCTGGAGGTATCAGATG	5	0.125	No Hit
ACACTCTTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAA	5	0.125	No Hit
TATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCC	5	0.125	No Hit
TAAAAACCGTTGCACAAGCTTTGCACTCTTCTATGCTCTGCTTTGTAGAC	5	0.125	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	5	0.125	No Hit
CTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGG	5	0.125	No Hit
GAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGAC	5	0.125	No Hit
ATTCCAATAGTATGTGGCCAGCTTATCAAAGTTGTGCACACAATTTGAAC	5	0.125	No Hit
CCAGCCGGGACGTCGGGCCGAGCCAAAGCGGAGTCTCGCATCCGGCGGCC	5	0.125	No Hit
TTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCATG	5	0.125	No Hit
GTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGG	5	0.125	No Hit
GCCAAGAAGATGGACAAAATGGTGCAGAAAAAGAATGCGGCTGGCGCATT	5	0.125	No Hit
AAAAACCGTTGCACAAGCTTTGCACTCTTCTATGCTCTGCTTTGTAGACC	5	0.125	No Hit
GAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTACTACAGC	5	0.125	No Hit
GGATCCTCTGGCATTGGAATTACAAAAAGCTGTGGGTGCTGGGACCTGAA	5	0.125	No Hit
CTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAATCT	5	0.125	No Hit
CTCAGCTGAGTTCACAGAAACTTCGTGGCAGCGGTGTTGCTGTTGAAACA	5	0.125	No Hit
GAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTT	5	0.125	No Hit
CTATGAACTCTTGAAAATCTTGACAAAACTTCTGGAGGTATCAGATGATC	5	0.125	No Hit
GAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTC	5	0.125	No Hit
GTAAAGGCCACTTTTATAAATTAAGTGCTTCTGCCTGTGTTCAGACTCCT	5	0.125	No Hit
CACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTC	5	0.125	No Hit
ATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACA	5	0.125	No Hit
AAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAA	5	0.125	No Hit
CAAGAATCTGTCAAAGAGAAAGTAACAAGAATCATTCTTGCAGCATTCCG	5	0.125	No Hit
CATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAG	5	0.125	No Hit
CCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGA	5	0.125	No Hit
GGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGGTTCTT	5	0.125	No Hit
GATACGCATTCAGTACTAACATTTGTTAGCAGCACCTTACAACTTGCTCA	5	0.125	No Hit
GGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTT	5	0.125	No Hit
AAAGAAGTTCCGAGGACAAGCAAGAGATGCTTCAAACTGAAGGCAGCCAG	5	0.125	No Hit
TTTTCTCAAGAAAAAACAAGGTTCTTTTTTGATTTTCCGTAGAAAACTAA	5	0.125	No Hit
AGCACCGCGGGTCATGGCTGGCACGGCGCGCGGCTGCGGGACCAGCCTGG	5	0.125	No Hit
ATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAAT	5	0.125	No Hit
CAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTG	5	0.125	No Hit
GCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCA	5	0.125	No Hit
CCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAATGTTT	5	0.125	No Hit
GGAAAAGGCATAATATAAATTTTTAGAGTTACCAAATGTAGTGTGTATTC	5	0.125	No Hit
TACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAG	5	0.125	No Hit
CAAGAATTATCGCCAAGTTAGCTGCTTGGGGGAAAGAACTGATGGAAGGC	5	0.125	No Hit
ATTATCCCTAATAATGGGGATAATGCTTTTGAGTGTCAAAAAGTAATACT	5	0.125	No Hit
ATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCC	5	0.125	No Hit
CCAGATAGTATCGATGCTCATACATTTGATTTCGAAACCATCTCCCATCC	5	0.125	No Hit
CGAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTA	5	0.125	No Hit
GTGTGAGCACCTTCGTCGCTATAATATCATTCCTGTTCTATCAGACATCC	5	0.125	No Hit
TACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCC	5	0.125	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	5	0.125	No Hit
GTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAATCTAATATACTTA	5	0.125	No Hit
CTTCATTAAATCCATATTTTGGGATTCGTATGTAAAATGGAGTCTGCCCT	5	0.125	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	5	0.125	No Hit
CATTCGAGGTGCTGTGGATGCTGCTGTCCCAACTAACATCATTGCGGCCA	5	0.125	No Hit
CGCCGTTGTGCCGGCCGCCCGGAAGGCCACCGCCGCGGTTATTTTCCTTC	5	0.125	No Hit
CAAACTCAAATGTAACACAGAGCTTTTGGAGCTCCTTGTCTGAAGGAGGG	5	0.125	No Hit
GCCTGGCCAACCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGT	5	0.125	No Hit
ATTTTATTTTGACATTTTGAGATACAGACATAGCCGGGCGTGGTGGTGCA	5	0.125	No Hit
ATGCTCCCTGATGGCTTCTTTGGTCAGGTTTTTCCTCATCTCTTTGAGCT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
2026-04-17T04:32:50 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:22 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:23 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:24 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:30 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:31 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:32 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:34 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:36 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:37 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:38 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:39 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:41 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:42 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
2026-04-17T04:33:43 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
2026-04-17T04:33:44 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
2026-04-17T04:33:52 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:53 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:33:55 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:34:00 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:34:01 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2026-04-17T04:34:02 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
2026-04-17T04:34:06 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1808621 spots for SRR32233440.sra
Written 1808621 spots for SRR32233440.sra
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
2026-04-17T04:34:08 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 1808615 spots for SRR32233440.sra
Written 1808615 spots for SRR32233440.sra
SRR ids: ['SRR32233440.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bj57_z5j
SRR32233440.sra spots: 14468926
blocks: [[1, 1808615], [1808616, 3617230], [3617231, 5425845], [5425846, 7234460], [7234461, 9043075], [9043076, 10851690], [10851691, 12660305], [12660306, 14468926]]
SRR32233440 file size 4867224
SRR32233440 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32233440 SRR32233440_1.fastq SRR32233440_2.fastq
Input file:	SRR32233440_1.fastq
Paired file:	SRR32233440_2.fastq
trimmed:	SRR32233440-trimmed-pair1.fastq, SRR32233440-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:35:06 2026 >> started

Fri Apr 17 04:35:51 2026 >> done (45.438s)
14468926 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
      52 ( 0.00%) empty read pairs filtered out after trimming by size control
14468874 (100.00%) read pairs available; of these:
  286001 ( 1.98%) trimmed read pairs available after processing
14182873 (98.02%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 53	       1	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       1	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       2	  0.00%
 66	       0	  0.00%
 67	       1	  0.00%
 68	       2	  0.00%
 69	       1	  0.00%
 70	       4	  0.00%
 71	       6	  0.00%
 72	       1	  0.00%
 73	       2	  0.00%
 74	       2	  0.00%
 75	       6	  0.00%
 76	       3	  0.00%
 77	      11	  0.00%
 78	       5	  0.00%
 79	       8	  0.00%
 80	       8	  0.00%
 81	       8	  0.00%
 82	       8	  0.00%
 83	      11	  0.00%
 84	       6	  0.00%
 85	      13	  0.00%
 86	      11	  0.00%
 87	      18	  0.00%
 88	      21	  0.00%
 89	       9	  0.00%
 90	      20	  0.00%
 91	      27	  0.00%
 92	      31	  0.00%
 93	      33	  0.00%
 94	      37	  0.00%
 95	      30	  0.00%
 96	      41	  0.00%
 97	      37	  0.00%
 98	      46	  0.00%
 99	      61	  0.00%
100	      41	  0.00%
101	      50	  0.00%
102	      57	  0.00%
103	      66	  0.00%
104	      71	  0.00%
105	      76	  0.00%
106	      94	  0.00%
107	      82	  0.00%
108	      80	  0.00%
109	     103	  0.00%
110	     100	  0.00%
111	     102	  0.00%
112	     123	  0.00%
113	     127	  0.00%
114	     105	  0.00%
115	     130	  0.00%
116	     134	  0.00%
117	     152	  0.00%
118	     193	  0.00%
119	     174	  0.00%
120	     181	  0.00%
121	     187	  0.00%
122	     190	  0.00%
123	     224	  0.00%
124	     218	  0.00%
125	     225	  0.00%
126	     277	  0.00%
127	     251	  0.00%
128	     290	  0.00%
129	     292	  0.00%
130	     323	  0.00%
131	     340	  0.00%
132	     384	  0.00%
133	     406	  0.00%
134	     392	  0.00%
135	     430	  0.00%
136	     519	  0.00%
137	     519	  0.00%
138	     519	  0.00%
139	     546	  0.00%
140	     651	  0.00%
141	   27103	  0.19%
142	   27354	  0.19%
143	   27851	  0.19%
144	   29619	  0.20%
145	   31132	  0.22%
146	   32061	  0.22%
147	   32649	  0.23%
148	   33710	  0.23%
149	   34566	  0.24%
150	14182873	 98.02%
14468874 reads passed initial QC


criterion=sequence-density
sequence-density=6.72
sequence-density-rank=1
fanout-score=2.18
fanout-score-rank=35
prefix-density=7.18
prefix-fanout=2.0
sequence=TGTTGATCCAATAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=41
fanout-score=97.91
fanout-score-rank=1
prefix-density=0.61
prefix-fanout=1.3
sequence=TTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCATGATGAGAACAGCTGTTAGTGAAATAAATGATCCTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCCCAGGAAATGTTGAGGGAAGAATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGTGAACAAATCCTGCTATGATAGCAAACACTGCTCCCATTGATAGAACATAGTGGAAATGGGCTACTACATAGTATGTA


criterion=sequence-density
sequence-density=1.10
sequence-density-rank=1
fanout-score=3.20
fanout-score-rank=29
prefix-density=3.54
prefix-fanout=1.0
sequence=TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAGGA


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=13
fanout-score=48.75
fanout-score-rank=1
prefix-density=5.07
prefix-fanout=1.0
sequence=ATAGAACTTCCCATCCGTATATT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAA -y TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAGGA -o SRR32233440 SRR32233440_1.fastq SRR32233440_2.fastq
Input file:	SRR32233440_1.fastq
Paired file:	SRR32233440_2.fastq
trimmed:	SRR32233440-trimmed-pair1.fastq, SRR32233440-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAA
-- paired 3' end adapter sequence (-y):	TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Fri Apr 17 04:37:16 2026 >> started

Fri Apr 17 04:37:31 2026 >> done (15.702s)
8681325 read pairs processed; of these:
     50 ( 0.00%) short read pairs filtered out after trimming by size control
    220 ( 0.00%) empty read pairs filtered out after trimming by size control
8681055 (100.00%) read pairs available; of these:
      6 ( 0.00%) trimmed read pairs available after processing
8681049 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 60	      1	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      1	  0.00%
 66	      0	  0.00%
 67	      1	  0.00%
 68	      0	  0.00%
 69	      1	  0.00%
 70	      2	  0.00%
 71	      5	  0.00%
 72	      1	  0.00%
 73	      1	  0.00%
 74	      2	  0.00%
 75	      3	  0.00%
 76	      3	  0.00%
 77	      5	  0.00%
 78	      2	  0.00%
 79	      5	  0.00%
 80	      3	  0.00%
 81	      5	  0.00%
 82	      2	  0.00%
 83	      6	  0.00%
 84	      4	  0.00%
 85	     10	  0.00%
 86	      8	  0.00%
 87	     13	  0.00%
 88	     14	  0.00%
 89	      4	  0.00%
 90	      9	  0.00%
 91	     17	  0.00%
 92	     19	  0.00%
 93	     19	  0.00%
 94	     24	  0.00%
 95	     18	  0.00%
 96	     26	  0.00%
 97	     18	  0.00%
 98	     33	  0.00%
 99	     38	  0.00%
100	     16	  0.00%
101	     22	  0.00%
102	     25	  0.00%
103	     36	  0.00%
104	     38	  0.00%
105	     41	  0.00%
106	     62	  0.00%
107	     49	  0.00%
108	     57	  0.00%
109	     68	  0.00%
110	     71	  0.00%
111	     66	  0.00%
112	     73	  0.00%
113	     82	  0.00%
114	     66	  0.00%
115	     79	  0.00%
116	     85	  0.00%
117	     99	  0.00%
118	    120	  0.00%
119	    101	  0.00%
120	     98	  0.00%
121	    104	  0.00%
122	    116	  0.00%
123	    133	  0.00%
124	    130	  0.00%
125	    146	  0.00%
126	    177	  0.00%
127	    145	  0.00%
128	    173	  0.00%
129	    178	  0.00%
130	    197	  0.00%
131	    212	  0.00%
132	    240	  0.00%
133	    233	  0.00%
134	    234	  0.00%
135	    259	  0.00%
136	    305	  0.00%
137	    299	  0.00%
138	    309	  0.00%
139	    312	  0.00%
140	    366	  0.00%
141	  15911	  0.18%
142	  16329	  0.19%
143	  16123	  0.19%
144	  17573	  0.20%
145	  18231	  0.21%
146	  18861	  0.22%
147	  19089	  0.22%
148	  20364	  0.23%
149	  20485	  0.24%
150	8512144	 98.05%


criterion=sequence-density
sequence-density=1.87
sequence-density-rank=1
fanout-score=2.28
fanout-score-rank=35
prefix-density=2.03
prefix-fanout=2.1
sequence=TGTTGATCCAATAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=39
fanout-score=72.91
fanout-score-rank=1
prefix-density=0.17
prefix-fanout=9.3
sequence=CTGGGCAGATGGGGGTGTTGAGATACCAGTGGACAGAGTGAGAGGATAGCATGTCCTCCAGAGGCGCGGGGGTAGTGTCCCTGCCTGGGAGCCTAAGCCTGAATGCACTAAGGGCTGGCACCACAGACGGGCTCAGGGGAGGCCCGCCCACAAGGGCTTCGGGCC


criterion=sequence-density
sequence-density=1.16
sequence-density-rank=1
fanout-score=2.21
fanout-score-rank=41
prefix-density=1.23
prefix-fanout=2.1
sequence=CCTAATCATATT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=222.78
fanout-score-rank=1
prefix-density=1.40
prefix-fanout=1.0
sequence=AATAGAACTTAAAATAAGCTTCAAAACTGACTTTTTCTCTATCCTGTTTACATCTGTAGCCCTTTTTGTCACATGATCAATTATACAATTCTCTTCATGATATATACACTCAGACCCAAACATCAATCGATTCATTAAATATCTTACACTATTCCTGATTACCATGCTTATCCTCACCTCAGCCAACAACATATTTCAACTTTTCATTGGCTGAGAAGGGGTGGGAATTATATCTTTCCTACTAATTGGATGATGGTACGGACGAACAGACGCAAATACTGCAGCCCTACAAGCAATCCTCTATAACCGCATCGGAGACATCGGATTCATTTTAGCTATAGTTTGATTTTCCCTAAACATAAACTCATGAGAACTTCAACAGATTATATTCTCCAACAACAACGACAATCTAATTCCACTTATAGGCCTATTAATCGCAGCTACAGGAAAATCAGCACAATTTGGCCTCCACCCATGACTACCATCAGCAATAGAAGGCCCTACACCAGTT
SRR32233440 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 17 04:38:37
                             Started mapping on |	Apr 17 04:38:37
                                    Finished on |	Apr 17 04:41:52
       Mapping speed, Million of reads per hour |	267.11

                          Number of input reads |	14468604
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	12005186
                        Uniquely mapped reads % |	82.97%
                          Average mapped length |	299.03
                       Number of splices: Total |	11140733
            Number of splices: Annotated (sjdb) |	11058729
                       Number of splices: GT/AG |	11040597
                       Number of splices: GC/AG |	79886
                       Number of splices: AT/AC |	9560
               Number of splices: Non-canonical |	10690
                      Mismatch rate per base, % |	0.18%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.68
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.27
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2356725
             % of reads mapped to multiple loci |	16.29%
        Number of reads mapped to too many loci |	12952
             % of reads mapped to too many loci |	0.09%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.62%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	106693	106693	106693
N_multimapping	2356725	2356725	2356725
N_noFeature	445319	10884930	1139957
N_ambiguous	608839	26033	176189
UnstrandedReadsAssigned:10951028 PositiveStrandReadsAssigned:1094223 NegativeStrandReadsAssigned:10689040
Dataset is classified negative stranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32233440 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32233440-trimmed-pair1.fastq
                             SRR32233440-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 14,468,604 reads, 12,747,140 reads pseudoaligned
[quant] estimated average fragment length: 223.521
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,043 rounds

 109283 SRR32233440.ke.tsv
  52636 SRR32233440.se.tsv
 161919 total
==> SRR32233440.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8.8	0	0
ENSMUST00000196221.1	9	2.75	0	0
ENSMUST00000179664.1	11	4.75	0	0
ENSMUST00000178537.1	12	4.8	0	0
ENSMUST00000178862.1	14	6.8	0	0
ENSMUST00000179520.1	11	4.75	0	0
ENSMUST00000179883.1	16	8.8	0	0
ENSMUST00000195858.1	10	3.75	0	0
ENSMUST00000179932.1	12	4.8	0	0

==> SRR32233440.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32233440 completed mapping pipeline successfully
