Starting /dee2/code/volunteer_pipeline.sh SRR32280100
    current disk space = 750634684
    free memory = 198099160 
SRR32280100 SRAfilesize
b8c50490d694724a232f5d4111851d46  SRR32280100.sra
SRR32280100.sra file validated
SRR32280100 is paired end
SRR32280100 is conventional basespace
SRR32280100 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32280100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.80475	38.0	37.0	38.0	36.0	38.0
2	36.7195	37.0	37.0	38.0	36.0	38.0
3	36.876	38.0	37.0	38.0	36.0	38.0
4	36.59125	37.0	37.0	38.0	36.0	38.0
5	36.72225	37.0	37.0	38.0	36.0	38.0
6	36.753	37.0	37.0	38.0	36.0	38.0
7	36.7405	37.0	37.0	38.0	36.0	38.0
8	36.867	37.0	37.0	38.0	36.0	38.0
9	36.94875	38.0	37.0	38.0	36.0	38.0
10-14	36.6886	37.0	37.0	38.0	36.0	38.0
15-19	36.75335	37.2	37.0	38.0	36.0	38.0
20-24	36.73585	37.0	37.0	38.0	36.0	38.0
25-29	36.686400000000006	37.0	37.0	38.0	36.0	38.0
30-34	36.637699999999995	37.0	37.0	38.0	36.0	38.0
35-39	36.671	37.0	37.0	38.0	36.0	38.0
40-44	36.69545000000001	37.0	37.0	38.0	36.0	38.0
45-49	36.62065	37.0	37.0	38.0	36.0	38.0
50-54	36.667449999999995	37.0	37.0	38.0	36.0	38.0
55-59	36.66224999999999	37.0	37.0	38.0	36.0	38.0
60-64	36.53955	37.0	37.0	38.0	35.6	38.0
65-69	36.4947	37.0	37.0	38.0	35.6	38.0
70-74	36.6175	37.0	37.0	38.0	35.8	38.0
75-79	36.58855	37.0	37.0	38.0	36.0	38.0
80-84	36.513099999999994	37.0	37.0	38.0	36.0	38.0
85-89	36.560900000000004	37.0	37.0	38.0	35.8	38.0
90-94	36.42895	37.0	37.0	38.0	35.4	38.0
95-99	36.42960000000001	37.0	37.0	38.0	35.8	38.0
100-104	36.307300000000005	37.0	37.0	38.0	35.0	38.0
105-109	36.46635	37.0	37.0	38.0	35.2	38.0
110-114	36.32775	37.0	37.0	38.0	34.8	38.0
115-119	36.32190000000001	37.0	37.0	38.0	35.0	38.0
120-124	36.284949999999995	37.0	37.0	38.0	35.0	38.0
125-129	36.210950000000004	37.0	37.0	38.0	34.4	38.0
130-134	36.151799999999994	37.0	37.0	38.0	34.4	38.0
135-139	36.05795	37.0	37.0	38.0	34.0	38.0
140-144	35.967349999999996	37.0	37.0	38.0	33.8	38.0
145-149	35.945299999999996	37.0	37.0	38.0	33.4	38.0
150	35.8445	37.0	37.0	38.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	6.0
26	16.0
27	38.0
28	37.0
29	41.0
30	48.0
31	49.0
32	80.0
33	117.0
34	132.0
35	239.0
36	736.0
37	2461.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.385096274068516	11.47786946736684	15.9039759939985	32.23305826456614
2	25.85646411602901	18.27956989247312	32.53313328332083	23.330832708177045
3	27.900000000000002	20.674999999999997	26.775	24.65
4	32.45	31.175000000000004	17.925	18.45
5	32.733183295823956	28.93223305826457	19.879969992498125	18.454613653413354
6	26.0	34.825	18.5	20.674999999999997
7	24.75	18.725	34.325	22.2
8	24.625	22.75	26.025	26.6
9	27.325	20.5	25.05	27.125
10-14	27.675	25.95	22.295	24.08
15-19	27.91	24.665	22.564999999999998	24.86
20-24	28.365000000000002	24.965	22.919999999999998	23.75
25-29	28.931446572328618	25.84629231461573	21.071053552677636	24.15120756037802
30-34	27.955000000000002	26.169999999999998	21.75	24.125
35-39	27.425	25.979999999999997	21.285	25.31
40-44	26.86	24.84	21.740000000000002	26.56
45-49	27.11	25.869999999999997	22.264999999999997	24.755
50-54	28.294999999999998	24.215	23.14	24.349999999999998
55-59	28.371418570928547	24.496224811240563	23.061153057652884	24.07120356017801
60-64	29.016450822541128	23.541177058852945	22.96114805740287	24.48122406120306
65-69	28.57	22.53	23.72	25.180000000000003
70-74	28.93	22.735	23.205000000000002	25.130000000000003
75-79	30.509999999999998	22.6	23.115	23.775
80-84	28.21	24.55	22.975	24.265
85-89	27.49	23.31	24.169999999999998	25.03
90-94	26.845000000000002	24.265	22.595000000000002	26.295
95-99	28.70143507175359	23.566178308915443	22.281114055702787	25.45127256362818
100-104	28.069210381557237	23.818572785917887	22.77841676251438	25.333800070010504
105-109	27.006350317515874	23.461173058652932	23.44617230861543	26.08630431521576
110-114	26.840000000000003	23.525	22.865	26.77
115-119	26.99539907981596	23.184636927385476	23.164632926585316	26.655331066213243
120-124	26.615	22.825	22.884999999999998	27.675
125-129	26.185237047409483	23.179635927185437	23.134626925385078	27.500500100020002
130-134	27.045	23.65	22.715	26.590000000000003
135-139	25.816290814540725	24.0062003100155	23.181159057952897	26.996349817490874
140-144	25.605	23.085	24.67	26.640000000000004
145-149	26.381319065953296	23.45617280864043	23.256162808140406	26.906345317265863
150	25.35	23.325000000000003	22.35	28.975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	1.5
21	1.0
22	2.0
23	3.5
24	4.0
25	3.5
26	4.5
27	7.5
28	10.0
29	8.0
30	8.5
31	16.0
32	20.5
33	20.5
34	24.5
35	29.5
36	35.0
37	41.5
38	43.0
39	44.5
40	48.0
41	50.5
42	53.0
43	62.5
44	58.0
45	58.5
46	64.5
47	65.0
48	80.0
49	87.5
50	129.0
51	153.0
52	202.5
53	337.0
54	324.0
55	202.0
56	188.5
57	217.5
58	195.5
59	145.5
60	142.5
61	144.0
62	167.0
63	164.5
64	89.0
65	54.5
66	63.5
67	58.0
68	22.5
69	7.0
70	3.5
71	3.0
72	5.0
73	3.5
74	2.5
75	4.0
76	2.5
77	2.0
78	2.5
79	1.0
80	0.5
81	1.0
82	1.0
83	2.0
84	1.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.025
3	0.0
4	0.0
5	0.025
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.005
60-64	0.005
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.005
100-104	0.015
105-109	0.005
110-114	0.0
115-119	0.02
120-124	0.0
125-129	0.02
130-134	0.0
135-139	0.005
140-144	0.0
145-149	0.005
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.38971387178559	58.25
2	7.569721115537849	10.45
3	2.8975009054690326	6.0
4	1.629844259326331	4.5
5	1.3038754074610648	4.5
6	0.5070626584570808	2.1
7	0.3621876131836291	1.7500000000000002
8	0.2535313292285404	1.4000000000000001
9	0.2897500905469033	1.7999999999999998
>10	0.796812749003984	9.25
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG	39	0.975	No Hit
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC	38	0.95	No Hit
GGGGTTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCAC	30	0.75	No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG	27	0.675	No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC	22	0.5499999999999999	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCACCTT	20	0.5	No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT	17	0.42500000000000004	No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT	17	0.42500000000000004	No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC	16	0.4	No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG	13	0.325	No Hit
CTTCACTTTGGCATTACCCATGATAGCAGAGGCAGAGGATAGGTCTCCAA	13	0.325	No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC	13	0.325	No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC	12	0.3	No Hit
CAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCA	11	0.27499999999999997	No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA	11	0.27499999999999997	No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT	11	0.27499999999999997	No Hit
GGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAGG	10	0.25	No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC	10	0.25	No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG	10	0.25	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	10	0.25	No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC	10	0.25	No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG	10	0.25	No Hit
CTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	9	0.22499999999999998	No Hit
GGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACG	9	0.22499999999999998	No Hit
GGGGGTTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCA	9	0.22499999999999998	No Hit
GTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCT	9	0.22499999999999998	No Hit
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAA	9	0.22499999999999998	No Hit
GGCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGA	9	0.22499999999999998	No Hit
TATCATTTTGCCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCAT	9	0.22499999999999998	No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG	9	0.22499999999999998	No Hit
GTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCC	8	0.2	No Hit
GCTTGTCACAGTGGAGCTCACTGAGGCTGGCAAAGGTGCCCTTGAGGCTG	8	0.2	No Hit
GTCACAGTGGAGCTCACTGAGGCTGGCAAAGGTGCCCTTGAGGCTGTCCA	8	0.2	No Hit
GTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAG	8	0.2	No Hit
CCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTG	8	0.2	No Hit
CCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTC	8	0.2	No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG	8	0.2	No Hit
GCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCAT	7	0.17500000000000002	No Hit
TGGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAG	7	0.17500000000000002	No Hit
CGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGC	7	0.17500000000000002	No Hit
CGCAGAAGGCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAG	7	0.17500000000000002	No Hit
GCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCA	7	0.17500000000000002	No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG	7	0.17500000000000002	No Hit
GCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCA	7	0.17500000000000002	No Hit
CTGGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAA	7	0.17500000000000002	No Hit
GTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTGATGCT	7	0.17500000000000002	No Hit
CGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAG	7	0.17500000000000002	No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC	6	0.15	No Hit
TTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCACCTTC	6	0.15	No Hit
CCTGTGCAGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATC	6	0.15	No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG	6	0.15	No Hit
CTTCTTGCCATGGGCCTTCACTTTGGCATTACCCATGATAGCAGAGGCAG	6	0.15	No Hit
CTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTATCCAGAGAGG	6	0.15	No Hit
ATTTTGCCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTT	6	0.15	No Hit
CGGCGACCTTCTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACA	6	0.15	No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT	6	0.15	No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT	6	0.15	No Hit
GGAAGATTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGG	6	0.15	No Hit
CTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGG	6	0.15	No Hit
GGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTG	6	0.15	No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG	6	0.15	No Hit
TGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAG	5	0.125	No Hit
GGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTA	5	0.125	No Hit
GCCTGTGCAGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAAT	5	0.125	No Hit
TGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGGAAG	5	0.125	No Hit
AGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCC	5	0.125	No Hit
GCTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCACCTT	5	0.125	No Hit
GCAGCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAA	5	0.125	No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG	5	0.125	No Hit
GGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAAGTTCTCA	5	0.125	No Hit
GGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTA	5	0.125	No Hit
GGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAAGG	5	0.125	No Hit
GTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAAC	5	0.125	No Hit
CGGCAAGAAGGTCGCCGATGCGCTGGCCAGTGCTGCAGGCCACCTCGATG	5	0.125	No Hit
CAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTTCACAGGC	5	0.125	No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA	5	0.125	No Hit
ACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAA	5	0.125	No Hit
TGGGCATGCAGGTCGCTCAGAGCAGACAAGGCACCGGGCAGGTCATCGAG	5	0.125	No Hit
CTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCA	5	0.125	No Hit
TTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTT	5	0.125	No Hit
ATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATATGGAGC	5	0.125	No Hit
AGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTTAAC	5	0.125	No Hit
GCAAGAGCAGGAAAGGGGGTTTAGTGGTACTTGTGAGCCAGGGCAGTGGC	5	0.125	No Hit
GCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTT	5	0.125	No Hit
CCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGC	5	0.125	No Hit
TGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGC	5	0.125	No Hit
TCCAAAGCTATCAAAGTACCGCTGGGTCCAAGGGTAGACAACCAGCAGCC	5	0.125	No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT	5	0.125	No Hit
CTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAGGCAGTTATCACCT	5	0.125	No Hit
CCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAA	5	0.125	No Hit
AGCACAATCACGATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCAC	5	0.125	No Hit
GGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCAGCTT	5	0.125	No Hit
GTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACG	5	0.125	No Hit
ACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACA	5	0.125	No Hit
GACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCA	5	0.125	No Hit
GTTGTGTTGACTTGCAACTTCAGAAACAGACATCATGGTGCACCTGACTG	5	0.125	No Hit
GTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCACCTTCTGGAAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32280100 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32280100_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.7265	38.0	37.0	38.0	36.0	38.0
2	36.63275	38.0	37.0	38.0	36.0	38.0
3	36.6935	38.0	37.0	38.0	36.0	38.0
4	36.71025	38.0	37.0	38.0	36.0	38.0
5	36.65025	37.0	37.0	38.0	36.0	38.0
6	36.58625	37.0	37.0	38.0	36.0	38.0
7	36.72875	37.0	37.0	38.0	36.0	38.0
8	36.49775	37.0	37.0	38.0	35.0	38.0
9	36.717	37.0	37.0	38.0	36.0	38.0
10-14	36.63805	37.2	37.0	38.0	35.8	38.0
15-19	36.579750000000004	37.0	37.0	38.0	35.8	38.0
20-24	36.5116	37.0	37.0	38.0	35.4	38.0
25-29	36.477850000000004	37.0	37.0	38.0	35.2	38.0
30-34	36.52569999999999	37.0	37.0	38.0	35.6	38.0
35-39	36.5167	37.0	37.0	38.0	35.6	38.0
40-44	36.48155	37.0	37.0	38.0	35.4	38.0
45-49	36.50055	37.0	37.0	38.0	35.6	38.0
50-54	36.52655	37.0	37.0	38.0	35.6	38.0
55-59	36.4454	37.0	37.0	38.0	35.4	38.0
60-64	36.4356	37.0	37.0	38.0	35.2	38.0
65-69	36.338049999999996	37.0	37.0	38.0	34.6	38.0
70-74	36.42045	37.0	37.0	38.0	35.2	38.0
75-79	36.36035	37.0	37.0	38.0	35.4	38.0
80-84	36.42	37.0	37.0	38.0	35.0	38.0
85-89	36.297250000000005	37.0	37.0	38.0	35.0	38.0
90-94	36.302550000000004	37.0	37.0	38.0	34.8	38.0
95-99	36.306799999999996	37.0	37.0	38.0	34.8	38.0
100-104	36.3167	37.0	37.0	38.0	35.0	38.0
105-109	36.30135	37.0	37.0	38.0	35.0	38.0
110-114	36.18435	37.0	37.0	38.0	34.4	38.0
115-119	36.06895	37.0	37.0	38.0	34.2	38.0
120-124	36.031600000000005	37.0	37.0	38.0	33.8	38.0
125-129	36.00865	37.0	37.0	38.0	33.8	38.0
130-134	35.9041	37.0	37.0	38.0	33.6	38.0
135-139	35.859750000000005	37.0	37.0	38.0	33.4	38.0
140-144	35.7767	37.0	37.0	38.0	32.6	38.0
145-149	35.6702	37.0	37.0	38.0	32.4	38.0
150	35.6925	37.0	37.0	38.0	32.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	10.0
25	24.0
26	34.0
27	34.0
28	34.0
29	48.0
30	49.0
31	61.0
32	80.0
33	116.0
34	154.0
35	247.0
36	642.0
37	2465.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.875	11.025	16.275000000000002	35.825
2	24.275	18.625	32.074999999999996	25.025
3	27.474999999999998	19.125	28.875	24.525
4	29.65	30.525000000000002	18.7	21.125
5	30.675	31.5	19.5	18.325
6	25.624999999999996	34.25	17.2	22.925
7	24.425	18.125	34.150000000000006	23.3
8	24.456114028507127	20.43010752688172	25.98149537384346	29.132283070767688
9	24.7	20.349999999999998	26.900000000000002	28.050000000000004
10-14	26.681334066703332	25.881294064703237	22.041102055102755	25.396269813490672
15-19	26.029999999999998	24.375	23.13	26.465
20-24	27.355	24.505	23.630000000000003	24.51
25-29	27.655	25.629999999999995	21.82	24.895
30-34	26.790000000000003	25.215	22.8	25.195
35-39	26.939999999999998	25.430000000000003	21.685	25.945
40-44	25.974999999999998	25.185000000000002	22.21	26.63
45-49	26.88134406720336	25.056252812640633	23.236161808090404	24.8262413120656
50-54	27.33	23.39	23.66	25.619999999999997
55-59	27.93	23.665	23.255	25.15
60-64	27.994999999999997	23.544999999999998	23.34	25.119999999999997
65-69	27.54	22.634999999999998	23.835	25.990000000000002
70-74	27.589999999999996	22.384999999999998	24.245	25.779999999999998
75-79	29.020000000000003	22.395	23.995	24.59
80-84	26.695	24.195	24.0	25.11
85-89	27.6	23.745	23.400000000000002	25.255
90-94	26.146307315365767	24.181209060453025	22.95114755737787	26.721336066803342
95-99	27.405	23.435	22.869999999999997	26.290000000000003
100-104	27.157715771577156	23.612361236123615	23.332333233323332	25.897589758975897
105-109	26.756337816890845	22.23111155557778	24.761238061903097	26.251312565628282
110-114	27.11	22.705000000000002	23.080000000000002	27.105
115-119	26.355	22.675	23.53	27.439999999999998
120-124	25.45	22.48	23.64	28.43
125-129	25.1	23.565	23.375	27.96
130-134	26.284999999999997	22.95	23.294999999999998	27.47
135-139	24.54	22.11	25.080000000000002	28.27
140-144	25.035	21.945	25.064999999999998	27.955000000000002
145-149	25.476273813690685	22.611130556527826	23.976198809940495	27.936396819840994
150	25.525	22.025	23.799999999999997	28.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.5
24	5.0
25	6.0
26	4.5
27	6.0
28	7.5
29	8.5
30	11.5
31	17.5
32	22.5
33	22.0
34	28.0
35	33.0
36	34.5
37	38.0
38	42.0
39	43.0
40	42.0
41	47.5
42	55.5
43	56.0
44	47.5
45	52.0
46	71.5
47	75.5
48	90.0
49	101.0
50	143.5
51	175.5
52	215.0
53	341.0
54	332.5
55	228.0
56	203.0
57	212.0
58	180.0
59	132.0
60	122.0
61	124.5
62	152.5
63	150.5
64	84.0
65	42.0
66	63.0
67	64.0
68	23.5
69	5.0
70	4.5
71	6.0
72	3.5
73	1.5
74	1.5
75	1.5
76	1.0
77	2.0
78	3.5
79	3.0
80	1.5
81	0.5
82	1.5
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.025
9	0.0
10-14	0.005
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.005
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.005
95-99	0.0
100-104	0.01
105-109	0.005
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.005
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.85
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.40595497458243	57.425000000000004
2	8.387799564270153	11.55
3	3.413217138707335	7.049999999999999
4	1.670297748729121	4.6
5	0.7262164124909223	2.5
6	0.6535947712418301	2.7
7	0.32679738562091504	1.575
8	0.2541757443718228	1.4000000000000001
9	0.29048656499636893	1.7999999999999998
>10	0.8714596949891068	9.4
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTG	32	0.8	No Hit
GACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAAC	29	0.7250000000000001	No Hit
CAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGC	25	0.625	No Hit
GTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCT	20	0.5	No Hit
GTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGT	19	0.475	No Hit
GTTTGCTAGCTTCCCCACCACCAAGACCTACTTCCCTCACTTTGATGTAA	18	0.44999999999999996	No Hit
GAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGC	18	0.44999999999999996	No Hit
TGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGC	17	0.42500000000000004	No Hit
CAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACAT	16	0.4	No Hit
CTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAA	14	0.35000000000000003	No Hit
CCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTC	14	0.35000000000000003	No Hit
GTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATG	14	0.35000000000000003	No Hit
CAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGC	13	0.325	No Hit
CTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAG	13	0.325	No Hit
GAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTG	13	0.325	No Hit
AAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCC	13	0.325	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCACCTT	12	0.3	No Hit
CATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTG	12	0.3	No Hit
TGGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAG	11	0.27499999999999997	No Hit
AAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGG	11	0.27499999999999997	No Hit
AGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCT	11	0.27499999999999997	No Hit
CTTCACTTTGGCATTACCCATGATAGCAGAGGCAGAGGATAGGTCTCCAA	11	0.27499999999999997	No Hit
CCAAGACCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTC	10	0.25	No Hit
CGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAGC	10	0.25	No Hit
GCCTGTGCAGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAAT	9	0.22499999999999998	No Hit
GGCAAAGGTGCCCTTGAGGCTGTCCAAGTGATTCAGGCCATCGTTAAAGG	9	0.22499999999999998	No Hit
TTGGTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTT	9	0.22499999999999998	No Hit
CTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGG	9	0.22499999999999998	No Hit
CCTACTTCCCTCACTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGT	9	0.22499999999999998	No Hit
CCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTG	9	0.22499999999999998	No Hit
CACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGG	9	0.22499999999999998	No Hit
CTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCA	9	0.22499999999999998	No Hit
CTTGTCACAGTGGAGCTCACTGAGGCTGGCAAAGGTGCCCTTGAGGCTGT	8	0.2	No Hit
CGGCAAGAAGGTCGCCGATGCGCTGGCCAGTGCTGCAGGCCACCTCGATG	8	0.2	No Hit
GCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGC	8	0.2	No Hit
GTCACAGTGGAGCTCACTGAGGCTGGCAAAGGTGCCCTTGAGGCTGTCCA	8	0.2	No Hit
GGGGTTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCAC	8	0.2	No Hit
GCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGGCA	8	0.2	No Hit
TGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGAT	8	0.2	No Hit
GCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTAGCTTCCCCAC	7	0.17500000000000002	No Hit
GGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTG	7	0.17500000000000002	No Hit
GTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGG	7	0.17500000000000002	No Hit
TGGGCCTTCACTTTGGCATTACCCATGATAGCAGAGGCAGAGGATAGGTC	7	0.17500000000000002	No Hit
GGGGGTTTAGTGGTACTTGTGAGCCAGGGCAGTGGCCACTCCAGCCACCA	7	0.17500000000000002	No Hit
CAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCT	7	0.17500000000000002	No Hit
GTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCA	7	0.17500000000000002	No Hit
GCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGCTGAATAT	7	0.17500000000000002	No Hit
CTTTGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGG	7	0.17500000000000002	No Hit
CAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAGCCA	6	0.15	No Hit
GGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCAGAGCAGACAAGG	6	0.15	No Hit
GGGCAGAGCCGTGGCTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTG	6	0.15	No Hit
CTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	6	0.15	No Hit
GCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGG	6	0.15	No Hit
CTTGAAGTTGACGGGATCCACACGCAGCTTGTGGGCATGCAGGTCGCTCA	6	0.15	No Hit
GTGAAATCGGCAGGGTGGTGGCTAGCCAAGGTCACCAGCAGGCAGTGGCT	6	0.15	No Hit
CCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGC	6	0.15	No Hit
CCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGC	6	0.15	No Hit
ATTTTGCCAACAACTGACAGATGCTCTCTTGGGAACAATTAACCATTGTT	6	0.15	No Hit
CTTACATCAAAGTGAGGGAAGTAGGTCTTGGTGGTGGGGAAGCTAGCAAA	6	0.15	No Hit
CCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCCCAGGAGCCTGAA	6	0.15	No Hit
CCACAAGCTGCGTGTGGATCCCGTCAACTTCAAGCTCCTGAGCCACTGCC	6	0.15	No Hit
CTTGCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAG	6	0.15	No Hit
GTGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCT	6	0.15	No Hit
GCCGTGACCCTTGACCTGGGCAGAGCCGTGGCTTACATCAAAGTGAGGGA	6	0.15	No Hit
CCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGGCTGCCTGGGGG	6	0.15	No Hit
GCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGG	6	0.15	No Hit
TGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAG	5	0.125	No Hit
GTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATCATATTGCC	5	0.125	No Hit
ATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGA	5	0.125	No Hit
GCCATCGTTAAAGGCAGTTATCACCTTCTTGCCATGGGCCTTCACTTTGG	5	0.125	No Hit
GTTAAAGGCAGTTATCACCTTCTTGCCATGGGCCTTCACTTTGGCATTAC	5	0.125	No Hit
GTCGCCGATGCGCTGGCCAGTGCTGCAGGCCACCTCGATGACCTGCCCGG	5	0.125	No Hit
GCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAG	5	0.125	No Hit
TCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAG	5	0.125	No Hit
CAGCGGGGGTGAAATCCTTGCCCAGGTGGTGGCCCAGCACAATCACGATC	5	0.125	No Hit
GTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGA	5	0.125	No Hit
CAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGATCCACACGCAGCTTGT	5	0.125	No Hit
GACAAAAGCAACATCAAGGCTGCCTGGGGGAAGATTGGTGGCCATGGTGC	5	0.125	No Hit
CGGCAAGAAGGTCGCCGATGCTCTGGCCACTGCTGCAGGCCACCTCGATG	5	0.125	No Hit
CTTCTTGCCATGGGCCTTCACTTTGGCATTACCCATGATAGCAGAGGCAG	5	0.125	No Hit
GTCCAGAGAGGCATGCACCGCGGGGGTGAAATCGGCAGGGTGGTGGCTAG	5	0.125	No Hit
ACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTT	5	0.125	No Hit
CATCGTTAAAGGCAGTTATCACCTTCTTGCCATGGGCCTTCACTTTGGCA	5	0.125	No Hit
TGGCCATGGTGCTGAATATGGAGCTGAAGCCCTGGAAAGGATGTTTGCTA	5	0.125	No Hit
GTGCAAGGGAGAGAAGAAGGGCATGGCCAGAAGGCAAGCCCCGCAGAAGG	5	0.125	No Hit
TCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAAGCAACATCAAGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
Read 3428073 spots for SRR32280100.sra
Written 3428073 spots for SRR32280100.sra
SRR ids: ['SRR32280100.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_zgpebhs6
SRR32280100.sra spots: 27424584
blocks: [[1, 3428073], [3428074, 6856146], [6856147, 10284219], [10284220, 13712292], [13712293, 17140365], [17140366, 20568438], [20568439, 23996511], [23996512, 27424584]]
SRR32280100 file size 9764520
SRR32280100 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32280100 SRR32280100_1.fastq SRR32280100_2.fastq
Input file:	SRR32280100_1.fastq
Paired file:	SRR32280100_2.fastq
trimmed:	SRR32280100-trimmed-pair1.fastq, SRR32280100-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Apr 13 08:53:07 2026 >> started

Mon Apr 13 08:53:54 2026 >> done (47.137s)
27424584 read pairs processed; of these:
       6 ( 0.00%) short read pairs filtered out after trimming by size control
       1 ( 0.00%) empty read pairs filtered out after trimming by size control
27424577 (100.00%) read pairs available; of these:
  507120 ( 1.85%) trimmed read pairs available after processing
26917457 (98.15%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 19	       4	  0.00%
 20	       5	  0.00%
 21	       3	  0.00%
 22	       4	  0.00%
 23	       1	  0.00%
 24	       5	  0.00%
 25	       3	  0.00%
 26	       1	  0.00%
 27	       4	  0.00%
 28	       3	  0.00%
 29	       4	  0.00%
 30	       4	  0.00%
 31	       2	  0.00%
 32	       4	  0.00%
 33	       5	  0.00%
 34	       3	  0.00%
 35	       4	  0.00%
 36	       5	  0.00%
 37	       4	  0.00%
 38	       2	  0.00%
 39	       1	  0.00%
 40	       5	  0.00%
 41	       2	  0.00%
 42	       1	  0.00%
 43	       7	  0.00%
 44	      10	  0.00%
 45	       5	  0.00%
 46	       3	  0.00%
 47	       6	  0.00%
 48	       4	  0.00%
 49	       7	  0.00%
 50	       1	  0.00%
 51	       8	  0.00%
 52	       1	  0.00%
 53	       3	  0.00%
 54	       2	  0.00%
 55	       4	  0.00%
 56	       2	  0.00%
 57	       1	  0.00%
 58	       3	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       1	  0.00%
 63	       2	  0.00%
 64	       3	  0.00%
 65	       1	  0.00%
 66	       1	  0.00%
 67	       0	  0.00%
 68	       3	  0.00%
 69	       0	  0.00%
 70	       1	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       1	  0.00%
 74	       1	  0.00%
 75	       1	  0.00%
 76	       0	  0.00%
 77	       1	  0.00%
 78	       4	  0.00%
 79	       2	  0.00%
 80	       0	  0.00%
 81	       0	  0.00%
 82	       0	  0.00%
 83	       0	  0.00%
 84	       1	  0.00%
 85	       1	  0.00%
 86	       1	  0.00%
 87	       0	  0.00%
 88	       0	  0.00%
 89	       0	  0.00%
 90	       0	  0.00%
 91	       0	  0.00%
 92	       0	  0.00%
 93	       0	  0.00%
 94	       0	  0.00%
 95	       0	  0.00%
 96	       0	  0.00%
 97	       0	  0.00%
 98	       0	  0.00%
 99	       0	  0.00%
100	       0	  0.00%
101	       0	  0.00%
102	       0	  0.00%
103	       0	  0.00%
104	       0	  0.00%
105	       0	  0.00%
106	       0	  0.00%
107	       0	  0.00%
108	       0	  0.00%
109	       0	  0.00%
110	       0	  0.00%
111	       0	  0.00%
112	       0	  0.00%
113	       0	  0.00%
114	       0	  0.00%
115	       0	  0.00%
116	       0	  0.00%
117	       0	  0.00%
118	       0	  0.00%
119	       0	  0.00%
120	       0	  0.00%
121	       0	  0.00%
122	       0	  0.00%
123	       0	  0.00%
124	       0	  0.00%
125	       0	  0.00%
126	       0	  0.00%
127	       0	  0.00%
128	       0	  0.00%
129	       0	  0.00%
130	       0	  0.00%
131	       0	  0.00%
132	       0	  0.00%
133	       0	  0.00%
134	       0	  0.00%
135	       0	  0.00%
136	       1	  0.00%
137	       0	  0.00%
138	       0	  0.00%
139	       0	  0.00%
140	       0	  0.00%
141	       5	  0.00%
142	      37	  0.00%
143	      30	  0.00%
144	      26	  0.00%
145	      22	  0.00%
146	      40	  0.00%
147	     245	  0.00%
148	    6151	  0.02%
149	  500392	  1.82%
150	26917457	 98.15%
27424577 reads passed initial QC


criterion=sequence-density
sequence-density=9.58
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=26
prefix-density=9.42
prefix-fanout=2.0
sequence=GCGGGGGTGAAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=27
fanout-score=666.00
fanout-score-rank=1
prefix-density=5.56
prefix-fanout=1.0
sequence=CCAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGC


criterion=sequence-density
sequence-density=7.26
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=26
prefix-density=7.15
prefix-fanout=2.0
sequence=GCGGGGGTGAAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=29
fanout-score=492.55
fanout-score-rank=1
prefix-density=4.36
prefix-fanout=1.0
sequence=CCAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GCGGGGGTGAAATC -y GCGGGGGTGAAATC -o SRR32280100 SRR32280100_1.fastq SRR32280100_2.fastq
Input file:	SRR32280100_1.fastq
Paired file:	SRR32280100_2.fastq
trimmed:	SRR32280100-trimmed-pair1.fastq, SRR32280100-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GCGGGGGTGAAATC
-- paired 3' end adapter sequence (-y):	GCGGGGGTGAAATC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Apr 13 08:56:33 2026 >> started

Mon Apr 13 08:57:06 2026 >> done (32.896s)
21330227 read pairs processed; of these:
   11086 ( 0.05%) short read pairs filtered out after trimming by size control
  117789 ( 0.55%) empty read pairs filtered out after trimming by size control
21201352 (99.40%) read pairs available; of these:
     105 ( 0.00%) trimmed read pairs available after processing
21201247 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 19	       5	  0.00%
 20	       4	  0.00%
 21	       1	  0.00%
 22	       1	  0.00%
 23	       1	  0.00%
 24	       4	  0.00%
 25	       2	  0.00%
 26	       0	  0.00%
 27	       4	  0.00%
 28	       3	  0.00%
 29	       2	  0.00%
 30	       4	  0.00%
 31	       1	  0.00%
 32	       3	  0.00%
 33	       4	  0.00%
 34	       3	  0.00%
 35	       4	  0.00%
 36	       4	  0.00%
 37	       4	  0.00%
 38	       2	  0.00%
 39	       1	  0.00%
 40	       3	  0.00%
 41	       2	  0.00%
 42	       1	  0.00%
 43	       5	  0.00%
 44	       9	  0.00%
 45	       4	  0.00%
 46	       1	  0.00%
 47	       6	  0.00%
 48	       4	  0.00%
 49	       6	  0.00%
 50	       1	  0.00%
 51	       6	  0.00%
 52	       1	  0.00%
 53	       2	  0.00%
 54	       1	  0.00%
 55	       3	  0.00%
 56	       2	  0.00%
 57	       1	  0.00%
 58	       3	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       2	  0.00%
 63	       2	  0.00%
 64	       2	  0.00%
 65	       1	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       2	  0.00%
 69	       0	  0.00%
 70	       1	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       1	  0.00%
 74	       1	  0.00%
 75	       1	  0.00%
 76	       0	  0.00%
 77	       1	  0.00%
 78	       3	  0.00%
 79	       1	  0.00%
 80	       0	  0.00%
 81	       0	  0.00%
 82	       0	  0.00%
 83	       1	  0.00%
 84	       1	  0.00%
 85	       1	  0.00%
 86	       1	  0.00%
 87	       0	  0.00%
 88	       0	  0.00%
 89	       0	  0.00%
 90	       0	  0.00%
 91	       0	  0.00%
 92	       0	  0.00%
 93	       0	  0.00%
 94	       0	  0.00%
 95	       0	  0.00%
 96	       0	  0.00%
 97	       0	  0.00%
 98	       0	  0.00%
 99	       0	  0.00%
100	       0	  0.00%
101	       0	  0.00%
102	       0	  0.00%
103	       0	  0.00%
104	       0	  0.00%
105	       0	  0.00%
106	       0	  0.00%
107	       0	  0.00%
108	       0	  0.00%
109	       0	  0.00%
110	       0	  0.00%
111	       0	  0.00%
112	       0	  0.00%
113	       0	  0.00%
114	       0	  0.00%
115	       0	  0.00%
116	       0	  0.00%
117	       0	  0.00%
118	       0	  0.00%
119	       0	  0.00%
120	       0	  0.00%
121	       0	  0.00%
122	       1	  0.00%
123	       0	  0.00%
124	       0	  0.00%
125	       0	  0.00%
126	       0	  0.00%
127	       0	  0.00%
128	       0	  0.00%
129	       0	  0.00%
130	       0	  0.00%
131	       0	  0.00%
132	       0	  0.00%
133	       0	  0.00%
134	       0	  0.00%
135	       0	  0.00%
136	       1	  0.00%
137	       0	  0.00%
138	       0	  0.00%
139	       0	  0.00%
140	       0	  0.00%
141	       3	  0.00%
142	      28	  0.00%
143	      21	  0.00%
144	      23	  0.00%
145	      20	  0.00%
146	      35	  0.00%
147	     212	  0.00%
148	    4768	  0.02%
149	  387452	  1.83%
150	20808648	 98.15%


criterion=sequence-density
sequence-density=9.40
sequence-density-rank=1
fanout-score=2.02
fanout-score-rank=26
prefix-density=9.46
prefix-fanout=2.0
sequence=GCGGGGGTGAAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=27
fanout-score=512.78
fanout-score-rank=1
prefix-density=5.71
prefix-fanout=1.0
sequence=AGCCAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTG


criterion=sequence-density
sequence-density=7.49
sequence-density-rank=1
fanout-score=2.02
fanout-score-rank=25
prefix-density=7.56
prefix-fanout=2.0
sequence=GCGGGGGTGAAATC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=28
fanout-score=509.60
fanout-score-rank=1
prefix-density=4.63
prefix-fanout=1.0
sequence=CCAGGGCAGAGCATATAAGGTGAGGTAGGATCAGTTGCTCCTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAAACAGACATCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTCTCTTGCCTGTGGGGAAAGGTGAACTCCGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGCCTCTGCTATCATGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAGGTGATAACTGCCTTTAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATCGTGATTGTGCTGGGCCACCACCTGGGCAAGGATTTCACCCCCGCTGCACAGGCTGCCTTCCAGAAGGTGGTGGCTGGAGTGGC
SRR32280100 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Apr 13 08:58:50
                             Started mapping on |	Apr 13 08:58:50
                                    Finished on |	Apr 13 09:08:57
       Mapping speed, Million of reads per hour |	161.89

                          Number of input reads |	27295702
                      Average input read length |	299
                                    UNIQUE READS:
                   Uniquely mapped reads number |	15362353
                        Uniquely mapped reads % |	56.28%
                          Average mapped length |	295.88
                       Number of splices: Total |	13721953
            Number of splices: Annotated (sjdb) |	13499526
                       Number of splices: GT/AG |	13631408
                       Number of splices: GC/AG |	56878
                       Number of splices: AT/AC |	5215
               Number of splices: Non-canonical |	28452
                      Mismatch rate per base, % |	0.49%
                         Deletion rate per base |	0.04%
                        Deletion average length |	2.17
                        Insertion rate per base |	0.01%
                       Insertion average length |	2.26
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	10960170
             % of reads mapped to multiple loci |	40.15%
        Number of reads mapped to too many loci |	55624
             % of reads mapped to too many loci |	0.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.23%
                     % of reads unmapped: other |	0.13%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	973179	973179	973179
N_multimapping	10960170	10960170	10960170
N_noFeature	2279876	8862658	8400196
N_ambiguous	925765	287342	295366
UnstrandedReadsAssigned:12156712 PositiveStrandReadsAssigned:6212353 NegativeStrandReadsAssigned:6666791
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
SRR32280100 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32280100-trimmed-pair1.fastq
                             SRR32280100-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 27,295,702 reads, 23,997,991 reads pseudoaligned
[quant] estimated average fragment length: 255.623
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 966 rounds

 109283 SRR32280100.ke.tsv
  52636 SRR32280100.se.tsv
 161919 total
==> SRR32280100.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	6	0	0
ENSMUST00000196221.1	9	2	0	0
ENSMUST00000179664.1	11	4	0	0
ENSMUST00000178537.1	12	5	0	0
ENSMUST00000178862.1	14	4	0	0
ENSMUST00000179520.1	11	4	0	0
ENSMUST00000179883.1	16	6	0	0
ENSMUST00000195858.1	10	3	0	0
ENSMUST00000179932.1	12	5	0	0

==> SRR32280100.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32280100 completed mapping pipeline successfully
