Starting /dee2/code/volunteer_pipeline.sh SRR32569733
    current disk space = 738545276
    free memory = 189717856 
SRR32569733 SRAfilesize
a5eb0b1b7d0135332135934e04f7ca31  SRR32569733.sra
SRR32569733.sra file validated
SRR32569733 is paired end
SRR32569733 is conventional basespace
SRR32569733 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.685	26.0	12.0	34.0	12.0	34.0
2	32.7895	34.0	34.0	34.0	34.0	34.0
3	33.048	34.0	34.0	34.0	34.0	34.0
4	33.1165	34.0	34.0	34.0	34.0	34.0
5	33.289	34.0	34.0	34.0	34.0	34.0
6	33.1675	34.0	34.0	34.0	34.0	34.0
7	32.996	34.0	34.0	34.0	34.0	34.0
8	33.2165	34.0	34.0	34.0	34.0	34.0
9	33.0125	34.0	34.0	34.0	34.0	34.0
10-11	32.9945	34.0	34.0	34.0	34.0	34.0
12-13	33.06425	34.0	34.0	34.0	34.0	34.0
14-15	33.114000000000004	34.0	34.0	34.0	34.0	34.0
16-17	33.04675	34.0	34.0	34.0	34.0	34.0
18-19	32.92825	34.0	34.0	34.0	34.0	34.0
20-21	33.0355	34.0	34.0	34.0	34.0	34.0
22-23	32.908	34.0	34.0	34.0	34.0	34.0
24-25	32.843	34.0	34.0	34.0	34.0	34.0
26-27	32.932500000000005	34.0	34.0	34.0	34.0	34.0
28-29	32.9935	34.0	34.0	34.0	34.0	34.0
30-31	33.05175	34.0	34.0	34.0	34.0	34.0
32-33	32.918499999999995	34.0	34.0	34.0	34.0	34.0
34-35	32.8285	34.0	34.0	34.0	34.0	34.0
36-37	32.920249999999996	34.0	34.0	34.0	34.0	34.0
38-39	32.82075	34.0	34.0	34.0	34.0	34.0
40-41	32.823750000000004	34.0	34.0	34.0	34.0	34.0
42-43	32.75325	34.0	34.0	34.0	34.0	34.0
44-45	32.86925	34.0	34.0	34.0	34.0	34.0
46-47	32.778499999999994	34.0	34.0	34.0	34.0	34.0
48-49	32.69475	34.0	34.0	34.0	34.0	34.0
50-51	32.51225	34.0	34.0	34.0	30.0	34.0
52-53	32.56325	34.0	34.0	34.0	26.0	34.0
54-55	32.38475	34.0	34.0	34.0	26.0	34.0
56-57	32.45725	34.0	34.0	34.0	26.0	34.0
58-59	32.5445	34.0	34.0	34.0	30.0	34.0
60-61	32.59675	34.0	34.0	34.0	34.0	34.0
62-63	32.40325	34.0	34.0	34.0	26.0	34.0
64-65	32.416250000000005	34.0	34.0	34.0	26.0	34.0
66-67	32.3755	34.0	34.0	34.0	26.0	34.0
68-69	32.3685	34.0	34.0	34.0	26.0	34.0
70-71	32.399	34.0	34.0	34.0	26.0	34.0
72-73	32.36725	34.0	34.0	34.0	26.0	34.0
74-75	32.18175	34.0	34.0	34.0	26.0	34.0
76-77	32.105999999999995	34.0	34.0	34.0	26.0	34.0
78-79	32.17725	34.0	34.0	34.0	26.0	34.0
80-81	32.07325	34.0	34.0	34.0	26.0	34.0
82-83	32.2505	34.0	34.0	34.0	26.0	34.0
84-85	32.11025	34.0	34.0	34.0	26.0	34.0
86-87	32.20975	34.0	34.0	34.0	26.0	34.0
88-89	32.11825	34.0	34.0	34.0	26.0	34.0
90-91	31.963	34.0	34.0	34.0	26.0	34.0
92-93	31.87975	34.0	34.0	34.0	26.0	34.0
94-95	31.9135	34.0	34.0	34.0	26.0	34.0
96-97	32.047	34.0	34.0	34.0	26.0	34.0
98-99	31.7975	34.0	34.0	34.0	26.0	34.0
100-101	31.713	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	2.0
24	7.0
25	16.0
26	40.0
27	39.0
28	52.0
29	124.0
30	262.0
31	376.0
32	1084.0
33	1961.0
34	35.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.75	54.87499999999999	43.3	1.075
2	43.225	11.95	10.125	34.699999999999996
3	24.5	15.875	32.824999999999996	26.8
4	23.45	21.325	25.45	29.775000000000002
5	21.85	31.674999999999997	24.099999999999998	22.375
6	25.624999999999996	32.65	26.924999999999997	14.799999999999999
7	25.924999999999997	33.45	24.075	16.55
8	15.9	27.150000000000002	44.3	12.65
9	20.674999999999997	27.650000000000002	35.575	16.1
10-11	17.45	34.150000000000006	34.0625	14.3375
12-13	22.537499999999998	27.6	30.7625	19.1
14-15	18.8375	28.299999999999997	35.3125	17.549999999999997
16-17	19.787499999999998	29.425	34.2625	16.525000000000002
18-19	22.412499999999998	27.625	35.05	14.912500000000001
20-21	22.3375	28.549999999999997	32.05	17.0625
22-23	18.95	30.412499999999998	32.625	18.0125
24-25	20.3875	28.712500000000002	32.2625	18.637500000000003
26-27	20.837500000000002	30.275000000000002	30.875000000000004	18.0125
28-29	19.7125	28.3375	31.55	20.4
30-31	19.0	29.275000000000002	32.087500000000006	19.6375
32-33	17.3125	30.675	31.55	20.4625
34-35	17.375	30.125	33.5	19.0
36-37	17.8625	30.45	31.7625	19.925
38-39	16.5625	30.875000000000004	33.5625	19.0
40-41	18.475	29.0875	33.4875	18.95
42-43	18.875	29.175	32.35	19.6
44-45	18.45	33.85	28.7375	18.9625
46-47	18.35	32.2125	30.7375	18.7
48-49	18.2	32.8625	30.312499999999996	18.625
50-51	19.725	32.35	30.0	17.925
52-53	17.8	30.25	31.125000000000004	20.825
54-55	17.6125	34.275	30.337500000000002	17.775
56-57	18.65	31.2	30.662499999999998	19.4875
58-59	17.375	31.125000000000004	32.15	19.35
60-61	17.549999999999997	31.937500000000004	30.862499999999997	19.650000000000002
62-63	16.537499999999998	32.5125	31.900000000000002	19.05
64-65	18.6875	33.0	30.15	18.1625
66-67	17.2	31.8625	33.4375	17.5
68-69	19.5625	31.674999999999997	30.6375	18.125
70-71	17.349999999999998	32.525	30.8125	19.3125
72-73	18.825	31.3	29.812499999999996	20.0625
74-75	18.4	31.912499999999998	31.175000000000004	18.512500000000003
76-77	17.2125	32.225	33.75	16.8125
78-79	16.375	32.074999999999996	33.550000000000004	18.0
80-81	18.275	33.9625	31.0375	16.725
82-83	17.1625	33.35	30.612499999999997	18.875
84-85	19.5	28.6875	34.825	16.9875
86-87	18.875	30.3875	31.587500000000002	19.15
88-89	20.7875	30.412499999999998	31.887500000000003	16.9125
90-91	19.6875	31.724999999999998	30.312499999999996	18.275
92-93	18.4125	30.8	31.5625	19.225
94-95	16.8	32.9625	32.8625	17.375
96-97	18.387500000000003	30.525000000000002	32.775	18.3125
98-99	18.987499999999997	32.4125	30.362499999999997	18.2375
100-101	19.0125	32.9125	30.837500000000002	17.2375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	13.0
18	17.5
19	9.0
20	19.0
21	23.0
22	21.5
23	18.0
24	12.0
25	21.0
26	28.0
27	47.5
28	76.0
29	112.5
30	167.5
31	223.5
32	229.5
33	199.0
34	192.5
35	173.5
36	133.5
37	122.0
38	157.5
39	185.0
40	164.0
41	204.0
42	249.5
43	215.0
44	188.0
45	170.0
46	116.0
47	75.0
48	76.0
49	100.0
50	108.0
51	65.5
52	30.5
53	15.5
54	7.5
55	3.0
56	0.0
57	0.0
58	0.0
59	0.0
60	2.0
61	2.0
62	0.0
63	0.5
64	0.5
65	1.0
66	1.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.5
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.411910669975185	29.425
2	20.099255583126553	20.25
3	9.627791563275434	14.549999999999999
4	4.267990074441688	8.6
5	2.7791563275434243	7.000000000000001
6	1.488833746898263	4.5
7	1.5384615384615385	5.425
8	0.39702233250620345	1.6
9	0.19851116625310172	0.8999999999999999
>10	1.1910669975186103	7.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	19	0.475	No Hit
TGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	18	0.44999999999999996	No Hit
AGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	17	0.42500000000000004	No Hit
TGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	17	0.42500000000000004	No Hit
ACCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	17	0.42500000000000004	No Hit
AGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTG	16	0.4	No Hit
TCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGG	15	0.375	No Hit
TGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	14	0.35000000000000003	No Hit
ACAGAAGAGGCAGTGGAAGGCCTCGAAGAACCATCACATTGTATAAGGTC	13	0.325	No Hit
AGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	13	0.325	No Hit
AGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	13	0.325	No Hit
AGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	12	0.3	No Hit
ACCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	12	0.3	No Hit
ACCCAGTTAAACCATTCCAAATCTCATCTTAATAATACCTGTTACTTATT	11	0.27499999999999997	No Hit
TCCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	11	0.27499999999999997	No Hit
TGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	11	0.27499999999999997	No Hit
AGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	11	0.27499999999999997	No Hit
TCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	10	0.25	No Hit
TACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAA	10	0.25	No Hit
ACCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	10	0.25	No Hit
TGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATC	10	0.25	No Hit
ACTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAAAGCCTTT	10	0.25	No Hit
ACATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGC	10	0.25	No Hit
AGGCAAATTAAGCAAATATGTACAGTCTCTTTGTGCCAGGAGGATGTGAC	10	0.25	No Hit
AGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGC	9	0.22499999999999998	No Hit
ATGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTCTTC	9	0.22499999999999998	No Hit
AGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTGG	9	0.22499999999999998	No Hit
AGCCATTATGTGGAGGAGCTGTTCAGCTCTCTCTAACCTATTTAGATTTT	9	0.22499999999999998	No Hit
TCTCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGG	8	0.2	No Hit
AGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	8	0.2	No Hit
AGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	8	0.2	No Hit
AGCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCAC	8	0.2	No Hit
TCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	8	0.2	No Hit
AGCAAGATTCTCATTAAGGCTAACTATGCTATTCTCCACTGCGCCCCATT	8	0.2	No Hit
AGTCAGCATGAATCCAGAGATAGAAAAAGTGGCAGGACTTTCTGGCTGCC	8	0.2	No Hit
TGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	8	0.2	No Hit
AGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	7	0.17500000000000002	No Hit
TGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	7	0.17500000000000002	No Hit
ACCCAGAAGAGGCAGTGGAAGGCCTCGAAGAACCATCACATTGTATAAGG	7	0.17500000000000002	No Hit
AGCCAGGCACTCTATTTGAGTAGATCAACATCTTTGCCAGCCAGCAGACC	7	0.17500000000000002	No Hit
ACCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTT	7	0.17500000000000002	No Hit
AGTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACC	7	0.17500000000000002	No Hit
ACCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	7	0.17500000000000002	No Hit
ACATTGAACCACATACCCTCTTGTCACTATACCTGGATGGAATATATTCC	7	0.17500000000000002	No Hit
TGTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACC	7	0.17500000000000002	No Hit
TGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	7	0.17500000000000002	No Hit
AGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCAT	7	0.17500000000000002	No Hit
ACACCGACTATGCATCAGAATACTATGTACAGTTGAAGTTAGGGAGCTGA	7	0.17500000000000002	No Hit
AACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGGG	7	0.17500000000000002	No Hit
ACACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGCAT	7	0.17500000000000002	No Hit
TGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	7	0.17500000000000002	No Hit
AGGGAAAAAATGAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGG	7	0.17500000000000002	No Hit
AGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	7	0.17500000000000002	No Hit
TTGGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATA	7	0.17500000000000002	No Hit
ACTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGAAGATAGTCATC	7	0.17500000000000002	No Hit
AGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTA	7	0.17500000000000002	No Hit
TGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	7	0.17500000000000002	No Hit
AGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	7	0.17500000000000002	No Hit
TGGAAAAAGTAAAACAAAAACAAAACCTTCAGTGTACATGTCAGCTGCTT	7	0.17500000000000002	No Hit
ACTCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGG	7	0.17500000000000002	No Hit
TCAGGCATTCATGGATAGAACATCTGAAGCACAGCCAAACATCCTCCTCC	7	0.17500000000000002	No Hit
ACAGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAG	7	0.17500000000000002	No Hit
AATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATATGA	7	0.17500000000000002	No Hit
AGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAA	7	0.17500000000000002	No Hit
TGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTG	7	0.17500000000000002	No Hit
TCCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	7	0.17500000000000002	No Hit
TCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGACT	7	0.17500000000000002	No Hit
ATGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATT	6	0.15	No Hit
ACAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTT	6	0.15	No Hit
AGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTCA	6	0.15	No Hit
TCCCACTTACTGTTCTTACATGACCTGACTATCCTAACAAACTCTATTCT	6	0.15	No Hit
ACTCCACTGCGCCCCATTGATCAGACAATGGCTTTAAGTGGGGCCTAAAT	6	0.15	No Hit
ATCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTTTC	6	0.15	No Hit
TTCTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCA	6	0.15	No Hit
TGCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCAC	6	0.15	No Hit
TATTTTTAGAAAGTAAATAAAAATAGGTTGCAGTTGCTCTATGTGATGTA	6	0.15	No Hit
ACTCCATGTTCCTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAG	6	0.15	No Hit
TATCAGAATACTATGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGT	6	0.15	No Hit
AGTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGA	6	0.15	No Hit
AGTCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGG	6	0.15	No Hit
ACAGGCATTCATGGATAGAACATCTGAAGCACAGCCAAACATCCTCCTCC	6	0.15	No Hit
TCCTGGTTTGTGGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTA	6	0.15	No Hit
AGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAA	6	0.15	No Hit
AGCATGTTGTTGGTACCTGTACCATTCTCTTCCATTGAGAATTCTAAGCC	6	0.15	No Hit
TGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGAG	6	0.15	No Hit
ACTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	6	0.15	No Hit
TCTGCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGCACTT	6	0.15	No Hit
AGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAA	6	0.15	No Hit
ATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCT	6	0.15	No Hit
TGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	6	0.15	No Hit
ACTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGACT	6	0.15	No Hit
AGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	6	0.15	No Hit
TCCAGGCATTCATGGATAGAACATCTGAAGCACAGCCAAACATCCTCCTC	6	0.15	No Hit
ACCGGTCATGTATTTTTAGAAAGTAAATAAAAATAGGTTGCAGTTGCTCT	6	0.15	No Hit
AGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	6	0.15	No Hit
TGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTGG	6	0.15	No Hit
AGGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTTCTTTGTTGCTATC	6	0.15	No Hit
TGTTGCTTGTGTTGTTTTAATTTTTTTTGAAAATTGTTTTGCACAAAATA	5	0.125	No Hit
AAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTTAACA	5	0.125	No Hit
AGGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTTA	5	0.125	No Hit
ACTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTTT	5	0.125	No Hit
AGGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGAT	5	0.125	No Hit
TTGTACATGTCAGCTGCTTTTGAAGCATCATAAGTACCTGGTTTGTGGCC	5	0.125	No Hit
TCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	5	0.125	No Hit
TCCAGTTAAACCATTCCAAATCTCATCTTAATAATACCTGTTACTTATTG	5	0.125	No Hit
TGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGGT	5	0.125	No Hit
AGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATC	5	0.125	No Hit
AAGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAGA	5	0.125	No Hit
TCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTCTTCCA	5	0.125	No Hit
TCTCAGAGCAAGTTGTACAGCTAAAGTGCCCTTTTGGGGGAATTGGTCAC	5	0.125	No Hit
ACCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAAA	5	0.125	No Hit
TCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	5	0.125	No Hit
AGGCTAGAGCACTTGGAGTCACTGCCCAGGGCAGCAGCCTCCAACCAAGA	5	0.125	No Hit
ACTCATTTAAATTTTTGTTTCAAATAAAATTCCAAATACATTTGTACAAT	5	0.125	No Hit
TGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGGGGACTCTA	5	0.125	No Hit
AGGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGT	5	0.125	No Hit
AGTCATGCTTCAGAAAGTCTAATGTCGTGAATTAGAAATGTTTAGCCAAT	5	0.125	No Hit
AGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCT	5	0.125	No Hit
TCCGATGTTTATTTTTATGATAAAAAGGATATACTTTTGTATTTTGGGGT	5	0.125	No Hit
ACTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTG	5	0.125	No Hit
TGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	5	0.125	No Hit
AAGAAGAGGCAGTGGAAGGCCTCGAAGAACCATCACATTGTATAAGGTCC	5	0.125	No Hit
TGGCTAACTATGCTATTCTCCACTGCGCCCCATTGATCAGACAATGGCTT	5	0.125	No Hit
ACAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAAAT	5	0.125	No Hit
ATGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATAT	5	0.125	No Hit
TGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAA	5	0.125	No Hit
AGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAA	5	0.125	No Hit
TGTGTATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCAT	5	0.125	No Hit
AGGTGTATGTATTGAAGCCATTATAAAAAATGACATTATTTATCATTATT	5	0.125	No Hit
ACTTGCCATCTGGAAGCAGGCTCACCAAGTATGATGGTTTCATACCCAGA	5	0.125	No Hit
ACCTTCTTCTTCCTGTGATGCACACGGTTCTCCATGTTCCTGCAATTTAG	5	0.125	No Hit
TACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGGG	5	0.125	No Hit
TCTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCA	5	0.125	No Hit
AGTTGGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATA	5	0.125	No Hit
AGGCCAGCAGACCAGAAATAGTAAGATCAACTGCCCAAGATTACCAGGAA	5	0.125	No Hit
TCAGAAGAGGCAGTGGAAGGCCTCGAAGAACCATCACATTGTATAAGGTC	5	0.125	No Hit
TTAGAGATAGAGTAGGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTT	5	0.125	No Hit
AGAATTGGGGACTCTACATGGCTCTGAAAAATGAGATCAGCCATACAAAG	5	0.125	No Hit
AGTCCAAAATAGTTAACGTACCAGGTAGACAAACACAGCTTAGTGATGAG	5	0.125	No Hit
ACTTCATTTCATCACAAATTGTAACTTACTTAACTGACCAAAAAAACTAT	5	0.125	No Hit
AGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTGTA	5	0.125	No Hit
TGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGC	5	0.125	No Hit
AGTTGCAATAAAATAAAAAAAAAATCTACAAAATTCAAATATATAAAATT	5	0.125	No Hit
ATGGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATA	5	0.125	No Hit
ACCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	5	0.125	No Hit
AATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCT	5	0.125	No Hit
TTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATAT	5	0.125	No Hit
AGGATTAGAAAGGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAA	5	0.125	No Hit
TCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTT	5	0.125	No Hit
AGTGCATTCTTATTTTATAATATTGACTCTATGACTCAAAAATTACAAGT	5	0.125	No Hit
AGCACACTTACAAGTTGAATTCTAAAGGAAGGTCTGGTTATGTGGGGTTG	5	0.125	No Hit
AGTTGCTTGTGTTGTTTTAATTTTTTTTGAAAATTGTTTTGCACAAAATA	5	0.125	No Hit
AGCAGTGGAAGGCCTCGAAGAACCATCACATTGTATAAGGTCCCCAGGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0125	0.0
76-77	0.0	0.0	0.0	0.037500000000000006	0.0
78-79	0.0	0.0	0.0	0.1125	0.0
80-81	0.0	0.0	0.0	0.16249999999999998	0.0
82-83	0.0	0.0	0.0	0.25	0.0
84-85	0.0	0.0	0.0	0.375	0.0
86-87	0.0	0.0	0.0	0.5	0.0
88-89	0.0	0.0	0.0	0.6125	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32569733 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569733_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	18.854	12.0	12.0	26.0	12.0	34.0
2	32.019	34.0	34.0	34.0	26.0	34.0
3	32.128	34.0	34.0	34.0	26.0	34.0
4	31.4145	34.0	34.0	34.0	26.0	34.0
5	32.229	34.0	34.0	34.0	26.0	34.0
6	31.9205	34.0	34.0	34.0	26.0	34.0
7	31.7785	34.0	34.0	34.0	26.0	34.0
8	32.2545	34.0	34.0	34.0	26.0	34.0
9	32.3505	34.0	34.0	34.0	26.0	34.0
10-11	32.16675	34.0	34.0	34.0	26.0	34.0
12-13	32.248	34.0	34.0	34.0	26.0	34.0
14-15	32.3215	34.0	34.0	34.0	26.0	34.0
16-17	32.3405	34.0	34.0	34.0	26.0	34.0
18-19	32.20425	34.0	34.0	34.0	26.0	34.0
20-21	32.202	34.0	34.0	34.0	26.0	34.0
22-23	32.207	34.0	34.0	34.0	26.0	34.0
24-25	32.0205	34.0	34.0	34.0	26.0	34.0
26-27	32.361999999999995	34.0	34.0	34.0	26.0	34.0
28-29	32.36325	34.0	34.0	34.0	26.0	34.0
30-31	32.3925	34.0	34.0	34.0	26.0	34.0
32-33	32.17775	34.0	34.0	34.0	26.0	34.0
34-35	32.068749999999994	34.0	34.0	34.0	26.0	34.0
36-37	32.28425	34.0	34.0	34.0	26.0	34.0
38-39	32.37825	34.0	34.0	34.0	26.0	34.0
40-41	32.275000000000006	34.0	34.0	34.0	26.0	34.0
42-43	32.268	34.0	34.0	34.0	26.0	34.0
44-45	32.224500000000006	34.0	34.0	34.0	26.0	34.0
46-47	32.03225	34.0	34.0	34.0	26.0	34.0
48-49	32.076750000000004	34.0	34.0	34.0	26.0	34.0
50-51	32.055	34.0	34.0	34.0	26.0	34.0
52-53	32.006	34.0	34.0	34.0	26.0	34.0
54-55	31.98375	34.0	34.0	34.0	26.0	34.0
56-57	31.972	34.0	34.0	34.0	26.0	34.0
58-59	31.9165	34.0	34.0	34.0	26.0	34.0
60-61	32.04775	34.0	34.0	34.0	26.0	34.0
62-63	31.9435	34.0	34.0	34.0	26.0	34.0
64-65	31.97325	34.0	34.0	34.0	26.0	34.0
66-67	31.75325	34.0	34.0	34.0	26.0	34.0
68-69	31.758499999999998	34.0	34.0	34.0	26.0	34.0
70-71	31.789749999999998	34.0	34.0	34.0	26.0	34.0
72-73	31.6685	34.0	34.0	34.0	26.0	34.0
74-75	31.542749999999998	34.0	34.0	34.0	26.0	34.0
76-77	31.588	34.0	34.0	34.0	26.0	34.0
78-79	31.67125	34.0	34.0	34.0	26.0	34.0
80-81	31.49025	34.0	34.0	34.0	26.0	34.0
82-83	31.486	34.0	34.0	34.0	26.0	34.0
84-85	31.44625	34.0	34.0	34.0	26.0	34.0
86-87	31.411	34.0	34.0	34.0	26.0	34.0
88-89	31.2595	34.0	34.0	34.0	26.0	34.0
90-91	31.095	34.0	34.0	34.0	19.0	34.0
92-93	31.24425	34.0	34.0	34.0	26.0	34.0
94-95	31.171	34.0	34.0	34.0	19.0	34.0
96-97	30.67425	34.0	34.0	34.0	12.0	34.0
98-99	30.9295	34.0	34.0	34.0	12.0	34.0
100-101	30.7175	34.0	34.0	34.0	12.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	3.0
23	7.0
24	12.0
25	30.0
26	45.0
27	99.0
28	128.0
29	230.0
30	408.0
31	767.0
32	1359.0
33	910.0
34	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.85	29.175	30.45	14.524999999999999
2	33.79327646763673	26.894129453085803	15.830406422478676	23.482187656798796
3	21.25	35.125	30.875000000000004	12.75
4	15.0	34.475	33.050000000000004	17.474999999999998
5	18.3	41.575	25.35	14.774999999999999
6	20.424999999999997	39.4	24.05	16.125
7	16.05	41.8	25.05	17.1
8	14.45	24.95	42.325	18.275
9	18.25	30.3	28.375	23.075000000000003
10-11	16.9875	34.4375	29.5	19.075
12-13	19.662499999999998	29.5	27.987499999999997	22.85
14-15	16.037499999999998	32.0375	32.7125	19.2125
16-17	18.525	30.0375	31.275	20.1625
18-19	17.4375	31.874999999999996	32.4125	18.275
20-21	17.7375	32.9625	30.362499999999997	18.9375
22-23	18.925	31.4	29.9625	19.7125
24-25	17.05	33.7	30.325000000000003	18.925
26-27	18.512500000000003	32.3125	30.0375	19.1375
28-29	19.2125	30.375000000000004	31.324999999999996	19.0875
30-31	18.75	32.125	30.275000000000002	18.85
32-33	17.025000000000002	32.2625	30.575000000000003	20.1375
34-35	17.62694694187666	31.214385209573255	33.13916677219197	18.01950107635811
36-37	18.637500000000003	32.3625	30.6375	18.3625
38-39	17.6125	30.45	31.75	20.1875
40-41	18.9	31.2625	30.2625	19.575
42-43	17.3875	30.9	32.7	19.0125
44-45	16.8	30.887500000000003	31.65	20.6625
46-47	17.974999999999998	30.475	31.15	20.4
48-49	16.5875	32.087500000000006	32.337500000000006	18.987499999999997
50-51	16.925	31.7875	32.0	19.287499999999998
52-53	17.65	31.7875	31.2625	19.3
54-55	17.8	31.85	31.9625	18.387500000000003
56-57	18.6875	31.587500000000002	30.112499999999997	19.6125
58-59	16.5125	32.275	31.4375	19.775000000000002
60-61	18.2375	31.2	31.5	19.0625
62-63	18.9	30.0	31.362499999999997	19.7375
64-65	17.2375	31.6	31.387500000000003	19.775000000000002
66-67	17.6125	30.6875	30.912499999999998	20.7875
68-69	19.5125	31.5	31.4	17.5875
70-71	18.75	31.162499999999998	31.937500000000004	18.15
72-73	18.375	31.2375	30.862499999999997	19.525000000000002
74-75	17.6375	31.8625	31.337500000000002	19.162499999999998
76-77	18.525	31.7	30.725	19.05
78-79	18.441135993994745	30.964594019767294	30.589265607406478	20.00500437883148
80-81	17.95	31.387500000000003	31.5375	19.125
82-83	17.6625	29.9375	32.8125	19.5875
84-85	17.6125	32.487500000000004	31.4625	18.4375
86-87	16.6375	32.1625	31.637500000000003	19.5625
88-89	18.6125	29.1875	32.925	19.275000000000002
90-91	18.1125	30.75	31.8625	19.275000000000002
92-93	18.8375	31.4875	30.5125	19.162499999999998
94-95	18.4	31.7375	29.9875	19.875
96-97	18.567103935418768	30.802219979818364	31.49596367305752	19.134712411705348
98-99	18.45	31.837500000000002	30.912499999999998	18.8
100-101	19.112499999999997	31.7375	30.862499999999997	18.2875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	9.0
18	13.5
19	6.5
20	7.0
21	12.0
22	12.0
23	17.0
24	26.5
25	35.5
26	50.5
27	68.0
28	81.5
29	107.5
30	136.0
31	166.5
32	179.0
33	183.5
34	191.0
35	184.0
36	197.5
37	188.5
38	167.5
39	210.0
40	231.0
41	220.0
42	251.0
43	236.0
44	183.0
45	148.5
46	97.5
47	68.5
48	73.5
49	70.0
50	60.0
51	44.5
52	28.0
53	18.5
54	8.0
55	2.0
56	3.0
57	2.0
58	1.5
59	0.5
60	0.0
61	1.0
62	1.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.35000000000000003
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.2874999999999999
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.08750000000000001
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.8999999999999999
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8874263941808	54.05
2	16.937997921718047	24.45
3	4.953238656044337	10.725
4	1.9397298233460338	5.6000000000000005
5	0.8659508139937652	3.125
6	0.17319016279875304	0.75
7	0.13855213023900242	0.7000000000000001
8	0.10391409767925182	0.6
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTAT	8	0.2	No Hit
TAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAG	8	0.2	No Hit
CTGTAAAGCTAAAGTTGTTTAAATTGTTTGATCTAAATACTTTGTGGTGA	8	0.2	No Hit
AGTACAATTCTACATTCAGAGCCAAGAATAACATAATGTGGCAGATGTTG	7	0.17500000000000002	No Hit
TAACACTCCCAAGTCCCTATGTAGTATTGTGATCATGTACAATTCTACAT	7	0.17500000000000002	No Hit
AAAGCAACCCAACTCACACCAGACTTGCTTTATTTGAATTTTTTAAAGCA	7	0.17500000000000002	No Hit
AAATACATCACATAGAGCAACTGCAACCTATTTTTATTTACTTTCTAAAA	7	0.17500000000000002	No Hit
AGAAAAACACACAAATTGTGTGACCAATTCCCCCAAAAGGGCACTTTAGC	6	0.15	No Hit
AGGTAGGAAAAACAACCTCAGCGAAGATCCTGTACAACTATTTCAAAACA	6	0.15	No Hit
TTGTATAGATGGACAAGCTGCTTGGATGTTGCAGGAGGAACTTAGTTTTC	6	0.15	No Hit
TGTAGAGTCCCCAATTCAGCCCTGGTGAGGCCAACTGATCCTCTGCTGGA	6	0.15	No Hit
ATGAAAATAAAAGTGTATTAGAAAAACCTAGCTTTTAGCATATGTGATTA	6	0.15	No Hit
TAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAAATCTAA	5	0.125	No Hit
TAACAACACAAGCAACCCAACTCACACCAGACTTGCTTTATTTGAATTTT	5	0.125	No Hit
GCGACATTAGACTTTCTGAAGCATGACTGTAGCATAGAAAATTATGTGAC	5	0.125	No Hit
TTTTTAAATGTTTCAGTGAAGTGCTTGTTCAAAGGCTTCAGCACCATATT	5	0.125	No Hit
TCACAGACACAAAGAGAAAAATCTTCTACTTCCTAAAAGAACTATTTCCC	5	0.125	No Hit
GGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCAC	5	0.125	No Hit
GAAAAGAGTGAGGATATTTCTTCACCTACCTCACAGTGTGTTATAAGGAA	5	0.125	No Hit
TGGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGT	5	0.125	No Hit
GGTTTGGTATGACTAGCAGGAGGTGGTGGAGAGAACATTCTATAGAGAGA	5	0.125	No Hit
AGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCAC	5	0.125	No Hit
GGAGCAACATAATATTGCTGGGGGGAAAAAGAGTTGAGTCTCTTTTCAGT	5	0.125	No Hit
AAAGAAATTCACCTTTCAAAAGGAGGGCTGAGGATATTCACTCTGCCCCA	5	0.125	No Hit
TACTTTCTGAAGCATGACTGTAGCATAGAAAATTATGTGACTCCTCAAAA	5	0.125	No Hit
TGCACAGAGCAACATAATATTGCTGGGGGGAAAAAGAGTTGAGTCTCTTT	5	0.125	No Hit
TAAAGAGTTGAGTCTCTTTTCAGTGGTTTCTATTCCACGTGGAATCCTCT	5	0.125	No Hit
TCATAGAGCAACTGCAACCTATTTTTATTTACTTTCTAAAAATACATGAC	5	0.125	No Hit
AGAAGTGCTTGTTCAAAGGCTTCAGCACCATATTGAAATATGGCTACTTG	5	0.125	No Hit
AATTGTTTTAAACGTGTTTAAAGATATTCTATAAAACAATATTCCCTTCA	5	0.125	No Hit
TCCTCCTGGCACAAAGAGACTGTACATATTTGCTTAATTTGCCTTAAATA	5	0.125	No Hit
GTGTACATAGTATTCTGATGCATAGTCGGTGACATTCTTGAGTTTCTCTC	5	0.125	No Hit
AAATTATTCCACTGCAAATTCCAAATGGTTATTTTGTGCAAAACAATTTT	5	0.125	No Hit
TCATAACCAGACCTTCCTTTAGAATTCAACTTGTAAGTGTGTCTTTCTTG	5	0.125	No Hit
TCACAAAGAGAAAAATCTTCTACTTCCTAAAAGAACTATTTCCCAGCTTA	5	0.125	No Hit
AGGCAACGTGAAGATATATCACCCGGACATCACGGTGCCTGGCCTCGGAT	5	0.125	No Hit
AGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0125	0.0
76-77	0.0	0.0	0.0	0.037500000000000006	0.0
78-79	0.0	0.0	0.0	0.1125	0.0
80-81	0.0	0.0	0.0	0.15	0.0
82-83	0.0	0.0	0.0	0.225	0.0
84-85	0.0	0.0	0.0	0.35	0.0
86-87	0.0	0.0	0.0	0.475	0.0
88-89	0.0	0.0	0.0	0.5875	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
Read 11086615 spots for SRR32569733.sra
Written 11086615 spots for SRR32569733.sra
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
Read 11086614 spots for SRR32569733.sra
Written 11086614 spots for SRR32569733.sra
SRR ids: ['SRR32569733.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_sbro85mg
SRR32569733.sra spots: 88692913
blocks: [[1, 11086614], [11086615, 22173228], [22173229, 33259842], [33259843, 44346456], [44346457, 55433070], [55433071, 66519684], [66519685, 77606298], [77606299, 88692913]]
SRR32569733 file size 21458652
SRR32569733 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32569733 SRR32569733_1.fastq SRR32569733_2.fastq
Input file:	SRR32569733_1.fastq
Paired file:	SRR32569733_2.fastq
trimmed:	SRR32569733-trimmed-pair1.fastq, SRR32569733-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 06:03:12 2026 >> started

Tue Mar 31 06:06:50 2026 >> done (218.437s)
88692913 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
88692913 (100.00%) read pairs available; of these:
    6271 ( 0.01%) trimmed read pairs available after processing
88686642 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 93	       1	  0.00%
 94	       1	  0.00%
 95	       2	  0.00%
 96	       9	  0.00%
 97	       8	  0.00%
 98	      43	  0.00%
 99	     465	  0.00%
100	    5742	  0.01%
101	88686642	 99.99%
88692913 reads passed initial QC


criterion=sequence-density
sequence-density=10.43
sequence-density-rank=1
fanout-score=2.12
fanout-score-rank=11
prefix-density=11.31
prefix-fanout=2.0
sequence=TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=876.40
fanout-score-rank=1
prefix-density=4.99
prefix-fanout=1.0
sequence=TATTAATAAGAGTCCTGCAGTA


criterion=sequence-density
sequence-density=1.97
sequence-density-rank=1
fanout-score=4.06
fanout-score-rank=21
prefix-density=7.75
prefix-fanout=1.0
sequence=TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=39
fanout-score=453.93
fanout-score-rank=1
prefix-density=4.39
prefix-fanout=1.0
sequence=CTTCCTTCCACTAGGAACTCCAA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATA -y TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAG -o SRR32569733 SRR32569733_1.fastq SRR32569733_2.fastq
Input file:	SRR32569733_1.fastq
Paired file:	SRR32569733_2.fastq
trimmed:	SRR32569733-trimmed-pair1.fastq, SRR32569733-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTG
-- paired 3' end adapter sequence (-y):	TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 06:12:47 2026 >> started

Tue Mar 31 06:15:00 2026 >> done (133.533s)
63352081 read pairs processed; of these:
     192 ( 0.00%) short read pairs filtered out after trimming by size control
     396 ( 0.00%) empty read pairs filtered out after trimming by size control
63351493 (100.00%) read pairs available; of these:
     361 ( 0.00%) trimmed read pairs available after processing
63351132 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	      12	  0.00%
 95	       2	  0.00%
 96	      12	  0.00%
 97	      11	  0.00%
 98	      72	  0.00%
 99	     403	  0.00%
100	    4422	  0.01%
101	63346559	 99.99%


criterion=sequence-density
sequence-density=1.51
sequence-density-rank=1
fanout-score=56.39
fanout-score-rank=3
prefix-density=1.89
prefix-fanout=45.1
sequence=ACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCATAGAAATCTGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=21
fanout-score=104.02
fanout-score-rank=1
prefix-density=0.23
prefix-fanout=9.9
sequence=CTCCACCTCCAGGGTGATGGTCTTGCC


criterion=sequence-density
sequence-density=1.52
sequence-density-rank=1
fanout-score=38.91
fanout-score-rank=2
prefix-density=1.96
prefix-fanout=30.1
sequence=ACTGTCTCTTATACACATCTGACGCTGCCGACGACCATCTCGCCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.11
sequence-density-rank=5
fanout-score=223.92
fanout-score-rank=1
prefix-density=0.94
prefix-fanout=25.9
sequence=CACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGA
SRR32569733 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 06:16:53
                             Started mapping on |	Mar 31 06:16:54
                                    Finished on |	Mar 31 06:26:34
       Mapping speed, Million of reads per hour |	550.50

                          Number of input reads |	88692325
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	80997838
                        Uniquely mapped reads % |	91.32%
                          Average mapped length |	198.50
                       Number of splices: Total |	45897721
            Number of splices: Annotated (sjdb) |	45455375
                       Number of splices: GT/AG |	45414143
                       Number of splices: GC/AG |	375472
                       Number of splices: AT/AC |	48830
               Number of splices: Non-canonical |	59276
                      Mismatch rate per base, % |	0.46%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.82
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.30
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4594979
             % of reads mapped to multiple loci |	5.18%
        Number of reads mapped to too many loci |	157646
             % of reads mapped to too many loci |	0.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.30%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3099508	3099508	3099508
N_multimapping	4594979	4594979	4594979
N_noFeature	5161589	77846885	5729905
N_ambiguous	3611767	154342	1061673
UnstrandedReadsAssigned:72224482 PositiveStrandReadsAssigned:2996611 NegativeStrandReadsAssigned:74206260
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32569733 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32569733-trimmed-pair1.fastq
                             SRR32569733-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 88,692,325 reads, 78,811,653 reads pseudoaligned
[quant] estimated average fragment length: 189.702
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,303 rounds

 109283 SRR32569733.ke.tsv
  52636 SRR32569733.se.tsv
 161919 total
==> SRR32569733.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9.25	0	0
ENSMUST00000196221.1	9	5.8	0	0
ENSMUST00000179664.1	11	6.83333	0	0
ENSMUST00000178537.1	12	7.83333	0	0
ENSMUST00000178862.1	14	9.83333	0	0
ENSMUST00000179520.1	11	6.83333	0	0
ENSMUST00000179883.1	16	9.25	0	0
ENSMUST00000195858.1	10	5.83333	0	0
ENSMUST00000179932.1	12	7.83333	0	0

==> SRR32569733.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	1936
ENSMUSG00000102851	0
ENSMUSG00000103377	2
ENSMUSG00000104017	0
ENSMUSG00000103025	1
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32569733 completed mapping pipeline successfully
