Starting /dee2/code/volunteer_pipeline.sh SRR32569740
    current disk space = 799716396
    free memory = 198961188 
SRR32569740 SRAfilesize
747f8813ed08f2ab2dedd4c0a8a826f8  SRR32569740.sra
SRR32569740.sra file validated
SRR32569740 is paired end
SRR32569740 is conventional basespace
SRR32569740 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.4725	26.0	12.0	34.0	12.0	34.0
2	32.863	34.0	34.0	34.0	34.0	34.0
3	33.172	34.0	34.0	34.0	34.0	34.0
4	33.0495	34.0	34.0	34.0	34.0	34.0
5	33.262	34.0	34.0	34.0	34.0	34.0
6	33.0215	34.0	34.0	34.0	34.0	34.0
7	32.9475	34.0	34.0	34.0	34.0	34.0
8	33.1085	34.0	34.0	34.0	34.0	34.0
9	33.0575	34.0	34.0	34.0	34.0	34.0
10-11	33.1315	34.0	34.0	34.0	34.0	34.0
12-13	32.99125	34.0	34.0	34.0	34.0	34.0
14-15	33.117999999999995	34.0	34.0	34.0	34.0	34.0
16-17	33.01225	34.0	34.0	34.0	34.0	34.0
18-19	33.117999999999995	34.0	34.0	34.0	34.0	34.0
20-21	32.957499999999996	34.0	34.0	34.0	34.0	34.0
22-23	32.949749999999995	34.0	34.0	34.0	34.0	34.0
24-25	32.866	34.0	34.0	34.0	34.0	34.0
26-27	33.036249999999995	34.0	34.0	34.0	34.0	34.0
28-29	32.9895	34.0	34.0	34.0	34.0	34.0
30-31	32.9625	34.0	34.0	34.0	34.0	34.0
32-33	32.913250000000005	34.0	34.0	34.0	34.0	34.0
34-35	32.939	34.0	34.0	34.0	34.0	34.0
36-37	32.92675	34.0	34.0	34.0	34.0	34.0
38-39	32.839	34.0	34.0	34.0	34.0	34.0
40-41	32.83425	34.0	34.0	34.0	34.0	34.0
42-43	32.90275	34.0	34.0	34.0	34.0	34.0
44-45	32.944	34.0	34.0	34.0	34.0	34.0
46-47	32.8035	34.0	34.0	34.0	34.0	34.0
48-49	32.73375	34.0	34.0	34.0	34.0	34.0
50-51	32.62125	34.0	34.0	34.0	30.0	34.0
52-53	32.646	34.0	34.0	34.0	34.0	34.0
54-55	32.560500000000005	34.0	34.0	34.0	26.0	34.0
56-57	32.54375	34.0	34.0	34.0	30.0	34.0
58-59	32.5665	34.0	34.0	34.0	26.0	34.0
60-61	32.457	34.0	34.0	34.0	26.0	34.0
62-63	32.3975	34.0	34.0	34.0	26.0	34.0
64-65	32.4795	34.0	34.0	34.0	26.0	34.0
66-67	32.47625	34.0	34.0	34.0	26.0	34.0
68-69	32.473	34.0	34.0	34.0	26.0	34.0
70-71	32.491749999999996	34.0	34.0	34.0	26.0	34.0
72-73	32.355999999999995	34.0	34.0	34.0	26.0	34.0
74-75	32.40675	34.0	34.0	34.0	26.0	34.0
76-77	32.26175	34.0	34.0	34.0	26.0	34.0
78-79	32.284	34.0	34.0	34.0	26.0	34.0
80-81	32.229	34.0	34.0	34.0	26.0	34.0
82-83	32.224000000000004	34.0	34.0	34.0	26.0	34.0
84-85	32.308	34.0	34.0	34.0	26.0	34.0
86-87	32.11325	34.0	34.0	34.0	26.0	34.0
88-89	32.152	34.0	34.0	34.0	26.0	34.0
90-91	32.113	34.0	34.0	34.0	26.0	34.0
92-93	32.050250000000005	34.0	34.0	34.0	26.0	34.0
94-95	31.98775	34.0	34.0	34.0	26.0	34.0
96-97	31.9805	34.0	34.0	34.0	26.0	34.0
98-99	31.841749999999998	34.0	34.0	34.0	26.0	34.0
100-101	31.7045	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	0.0
24	4.0
25	11.0
26	25.0
27	50.0
28	67.0
29	125.0
30	204.0
31	387.0
32	1091.0
33	2009.0
34	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.15	53.675	44.35	0.8250000000000001
2	37.724999999999994	12.4	11.525	38.35
3	22.85	17.525	35.85	23.775
4	21.725	21.8	25.45	31.025000000000002
5	21.15	31.474999999999998	25.3	22.075
6	26.525	29.95	27.400000000000002	16.125
7	23.075000000000003	33.175	28.325	15.425
8	16.375	25.424999999999997	45.5	12.7
9	18.55	27.975	37.525	15.950000000000001
10-11	15.712499999999999	34.0125	36.825	13.450000000000001
12-13	21.675	28.15	31.0	19.175
14-15	15.575	29.8875	37.2875	17.25
16-17	20.3625	29.075	33.5875	16.975
18-19	22.125	27.825	34.075	15.975
20-21	21.525	30.0	31.624999999999996	16.85
22-23	19.8	32.9125	30.7625	16.525000000000002
24-25	20.5375	29.475	31.837500000000002	18.15
26-27	20.125	28.799999999999997	31.2	19.875
28-29	18.375	29.312500000000004	32.2	20.1125
30-31	20.225	29.525000000000002	31.45	18.8
32-33	17.1375	29.7	33.637499999999996	19.525000000000002
34-35	16.525000000000002	32.15	34.112500000000004	17.2125
36-37	17.175	31.674999999999997	33.650000000000006	17.5
38-39	15.862499999999999	29.862499999999997	35.6875	18.587500000000002
40-41	19.2125	27.224999999999998	34.637499999999996	18.925
42-43	19.3375	30.3875	32.2875	17.9875
44-45	20.4125	32.6125	29.575000000000003	17.4
46-47	19.287499999999998	31.2125	32.7875	16.7125
48-49	19.287499999999998	31.362499999999997	32.225	17.125
50-51	17.7875	31.8	32.574999999999996	17.837500000000002
52-53	17.712500000000002	28.5875	33.050000000000004	20.65
54-55	19.25	32.6375	30.475	17.6375
56-57	17.8125	31.95	31.75	18.4875
58-59	16.5625	32.4125	32.6625	18.3625
60-61	16.950000000000003	33.550000000000004	29.862499999999997	19.6375
62-63	15.512500000000001	33.45	33.5625	17.474999999999998
64-65	18.8125	31.5125	32.0625	17.6125
66-67	16.5625	31.924999999999997	34.7125	16.8
68-69	18.6875	32.425	30.6875	18.2
70-71	14.975	33.125	31.4875	20.4125
72-73	17.0625	30.275000000000002	32.3875	20.275000000000002
74-75	16.675	33.2375	32.475	17.6125
76-77	17.075000000000003	31.6875	34.5875	16.650000000000002
78-79	18.2625	32.1	31.5375	18.099999999999998
80-81	16.55	32.9	34.5125	16.037499999999998
82-83	18.224999999999998	32.3875	31.85	17.5375
84-85	19.85	29.512500000000003	33.4	17.2375
86-87	18.7625	30.9875	31.9625	18.2875
88-89	19.4375	29.6625	33.037499999999994	17.8625
90-91	19.5625	31.8625	30.625000000000004	17.95
92-93	18.0	32.4	32.4125	17.1875
94-95	17.349999999999998	31.924999999999997	32.800000000000004	17.925
96-97	20.0	33.4125	29.9625	16.625
98-99	18.7375	33.6125	28.7375	18.912499999999998
100-101	17.299999999999997	33.175	31.35	18.175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	14.0
18	23.0
19	15.5
20	21.5
21	28.5
22	33.0
23	26.5
24	11.5
25	27.0
26	47.5
27	64.5
28	86.0
29	115.0
30	192.0
31	275.5
32	270.0
33	232.0
34	223.0
35	185.5
36	139.0
37	113.5
38	108.5
39	135.0
40	133.0
41	178.5
42	226.0
43	167.0
44	152.5
45	164.5
46	106.0
47	74.5
48	68.5
49	81.5
50	90.0
51	70.5
52	52.5
53	30.0
54	13.5
55	0.0
56	1.0
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.68041237113402	27.975
2	19.226804123711343	18.65
3	9.484536082474227	13.8
4	5.154639175257731	10.0
5	2.3195876288659796	5.625
6	2.1649484536082473	6.3
7	1.2886597938144329	4.375
8	0.979381443298969	3.8
9	0.4123711340206186	1.7999999999999998
>10	1.2886597938144329	7.675
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	22	0.5499999999999999	No Hit
TGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	18	0.44999999999999996	No Hit
AGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	17	0.42500000000000004	No Hit
TCTCATCTTAATAATACCTGTTACTTATTGTCCAGCAGAGGATCAGTTGG	16	0.4	No Hit
AGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	15	0.375	No Hit
TCTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATACA	14	0.35000000000000003	No Hit
ACCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	13	0.325	No Hit
TGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	12	0.3	No Hit
ACCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	12	0.3	No Hit
AGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	11	0.27499999999999997	No Hit
ACTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	11	0.27499999999999997	No Hit
TGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	11	0.27499999999999997	No Hit
ACTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATACA	11	0.27499999999999997	No Hit
TGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	11	0.27499999999999997	No Hit
TCTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGAAGATAGTCATC	11	0.27499999999999997	No Hit
AGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	11	0.27499999999999997	No Hit
ACTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTC	11	0.27499999999999997	No Hit
AGGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGAT	10	0.25	No Hit
AGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	10	0.25	No Hit
AGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	10	0.25	No Hit
ACTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTG	10	0.25	No Hit
TCTCATTTTTAACAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTT	10	0.25	No Hit
AGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAA	10	0.25	No Hit
TCCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	10	0.25	No Hit
AGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAA	10	0.25	No Hit
AGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTA	9	0.22499999999999998	No Hit
TCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	9	0.22499999999999998	No Hit
TCTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	9	0.22499999999999998	No Hit
TCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	9	0.22499999999999998	No Hit
TGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	9	0.22499999999999998	No Hit
TGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTA	9	0.22499999999999998	No Hit
ACTACCATCTCTCCACATTTTAAAGCCTTTGTGGAGCTCACATCTGTGTA	9	0.22499999999999998	No Hit
AGGAAAAAGTAAAACAAAAACAAAACCTTCAGTGTACATGTCAGCTGCTT	9	0.22499999999999998	No Hit
TGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	8	0.2	No Hit
ACTCATTTTTAACAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTT	8	0.2	No Hit
AGTGTATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCAT	8	0.2	No Hit
AGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	8	0.2	No Hit
TCTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	8	0.2	No Hit
AGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAA	8	0.2	No Hit
AGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCA	8	0.2	No Hit
TATTGGAAAGAGGAAGGAGAAAAGCACAAAATATTTAAGGCAAATTAAGC	8	0.2	No Hit
ACCTGCACACACCTTATTTCTGAAATGGAGAGAGAAACTCAAGAATGTCA	8	0.2	No Hit
AGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTT	8	0.2	No Hit
AGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	8	0.2	No Hit
ACTCATCTTAATAATACCTGTTACTTATTGTCCAGCAGAGGATCAGTTGG	8	0.2	No Hit
AGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	8	0.2	No Hit
ATACAGATTCCTTGCTTCTTTGTTGCTATCATTGAACCACATACCCTCTT	8	0.2	No Hit
AAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTCAAATT	8	0.2	No Hit
TTCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCT	8	0.2	No Hit
TCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGC	8	0.2	No Hit
AGGCAGTTGTATTTACAAATAATCACATATATGATGCTTACAGAATGATG	8	0.2	No Hit
ACCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	8	0.2	No Hit
AGTTCTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGT	7	0.17500000000000002	No Hit
TGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	7	0.17500000000000002	No Hit
ACTGAATGGAGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTT	7	0.17500000000000002	No Hit
AGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATT	7	0.17500000000000002	No Hit
AGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTCTTCC	7	0.17500000000000002	No Hit
ACAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTT	7	0.17500000000000002	No Hit
ACCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTT	7	0.17500000000000002	No Hit
AGTCCATATGAGGGATTAATTGTTCTCATTATAAAGTTTATAATTTCTGC	7	0.17500000000000002	No Hit
TCCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	7	0.17500000000000002	No Hit
AGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATC	7	0.17500000000000002	No Hit
TCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTC	7	0.17500000000000002	No Hit
TCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTG	7	0.17500000000000002	No Hit
ACCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	7	0.17500000000000002	No Hit
AATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGTAATATGG	7	0.17500000000000002	No Hit
ACCCACTTACTGTTCTTACATGACCTGACTATCCTAACAAACTCTATTCT	7	0.17500000000000002	No Hit
TGTGTATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCAT	7	0.17500000000000002	No Hit
TGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTCTT	7	0.17500000000000002	No Hit
AGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTA	7	0.17500000000000002	No Hit
ACACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	7	0.17500000000000002	No Hit
ACTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAAAGCCTTT	7	0.17500000000000002	No Hit
ACATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGC	7	0.17500000000000002	No Hit
TTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATAT	7	0.17500000000000002	No Hit
ACCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGGTA	7	0.17500000000000002	No Hit
TAGGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTT	7	0.17500000000000002	No Hit
AGCCATTATGTGGAGGAGCTGTTCAGCTCTCTCTAACCTATTTAGATTTT	7	0.17500000000000002	No Hit
TCCAGGGCTGAATTGGGGACTCTACATGGCTCTGAAAAATGAGATCAGCC	6	0.15	No Hit
TCTCAAAAATTACAAGTGTTTATAACCCTACTTAGTTTAGTCATTTAGGC	6	0.15	No Hit
AAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAATT	6	0.15	No Hit
TCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	6	0.15	No Hit
AGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTG	6	0.15	No Hit
AATCAGAATACTATGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGT	6	0.15	No Hit
TCACACTTACAAGTTGAATTCTAAAGGAAGGTCTGGTTATGTGGGGTTGG	6	0.15	No Hit
ACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATT	6	0.15	No Hit
TCCTAGACTTTTAATGGAAAGTTTTCACTGGCATAGTTGCTGCTTTTCAG	6	0.15	No Hit
AGTCACCGACTATGCATCAGAATACTATGTACAGTTGAAGTTAGGGAGCT	6	0.15	No Hit
TGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAA	6	0.15	No Hit
TCCTCTTGTCACTATACCTGGATGGAATATATTCCATACTGAAGGGAATA	6	0.15	No Hit
AATCCTATTTATGCAAGTAGCCATATTTCAATATGGTGCTGAAGCCTTTG	6	0.15	No Hit
ACCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	6	0.15	No Hit
TGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATT	6	0.15	No Hit
TCTCCTCTTTTGCTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGG	6	0.15	No Hit
ACCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTG	6	0.15	No Hit
AAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCA	6	0.15	No Hit
ACTCATTTAAATTTTTGTTTCAAATAAAATTCCAAATACATTTGTACAAT	6	0.15	No Hit
AGTTCTCTTCTGGTTTGTCTTCATCTACCAGCTATTACTCCTCTTTTGCT	6	0.15	No Hit
TGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	6	0.15	No Hit
AGGCATCCGCCCTTCTTCTTCCTGTGATGCACACGGTTCTCCATGTTCCT	6	0.15	No Hit
AGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	6	0.15	No Hit
TGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATC	6	0.15	No Hit
TCCTGGTTTGTGGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTA	6	0.15	No Hit
TCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCATAAAATT	6	0.15	No Hit
TCTTCATTTCATCACAAATTGTAACTTACTTAACTGACCAAAAAAACTAT	6	0.15	No Hit
TGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAA	6	0.15	No Hit
TCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	6	0.15	No Hit
ACTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	6	0.15	No Hit
ACTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGAAGATAGTCATC	6	0.15	No Hit
ACCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGG	6	0.15	No Hit
TCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTC	6	0.15	No Hit
AGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGTAATATGGTTGAC	6	0.15	No Hit
TATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGTAATATGG	6	0.15	No Hit
TCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTTCCTGTGATGCA	6	0.15	No Hit
TCTCCCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATAT	6	0.15	No Hit
TCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTT	6	0.15	No Hit
AATTGGAAAGAGGAAGGAGAAAAGCACAAAATATTTAAGGCAAATTAAGC	6	0.15	No Hit
AGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTCTT	6	0.15	No Hit
AGTTTACAAAGTAAGAGACAGGACTGGCAGGTTTGCATCCGAGGCCAGGC	6	0.15	No Hit
TCACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGT	6	0.15	No Hit
ACTTCAGTGTACATGTCAGCTGCTTTTGAAGCATCATAAGTACCTGGTTT	5	0.125	No Hit
TTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAA	5	0.125	No Hit
ACACTGTAGATATTTTTGAGGAGTCACATAATTTTCTATGCTACAGTCAT	5	0.125	No Hit
ACCCAGTTAAACCATTCCAAATCTCATCTTAATAATACCTGTTACTTATT	5	0.125	No Hit
AAGGAGATTATATTGGGTGTCAATACTGTAAGTATTCCAAGCACAACTTA	5	0.125	No Hit
AACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAA	5	0.125	No Hit
AGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAAATTCAAATAAA	5	0.125	No Hit
TGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCA	5	0.125	No Hit
ATGGACATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGTAA	5	0.125	No Hit
TAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCA	5	0.125	No Hit
ACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATA	5	0.125	No Hit
TACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAAC	5	0.125	No Hit
TCACAGATTGATGATCTCTAGGAAAAAGTAAAACAAAAACAAAACCTTCA	5	0.125	No Hit
ACTGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAA	5	0.125	No Hit
TACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAA	5	0.125	No Hit
AGCTCTCTCTAACCTATTTAGATTTTATCTTTCACTTTGTGATAGAATCT	5	0.125	No Hit
AGTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGA	5	0.125	No Hit
ACATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTTCCT	5	0.125	No Hit
AATTGACAACTTAATTTTAGAGAATCAGAGGAGATTATATTGGGTGTCAA	5	0.125	No Hit
AGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGC	5	0.125	No Hit
ACTTTGAACAAGCACTTCACTGAAACATTTAAAATACATTTATAATATGG	5	0.125	No Hit
TGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	5	0.125	No Hit
AATGGAATTTATTTTGTAAATGAATTTAGTTATTAAATAAGAGTACTGAA	5	0.125	No Hit
ACCTCTTGTCACTATACCTGGATGGAATATATTCCATACTGAAGGGAATA	5	0.125	No Hit
ATTCTTCTTCCTGTGATGCACACGGTTCTCCATGTTCCTGCAATTTAGTG	5	0.125	No Hit
ACCTATTTATGCAAGTAGCCATATTTCAATATGGTGCTGAAGCCTTTGAA	5	0.125	No Hit
TGCTCTCTCTAACCTATTTAGATTTTATCTTTCACTTTGTGATAGAATCT	5	0.125	No Hit
AGCTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATAC	5	0.125	No Hit
TGTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGA	5	0.125	No Hit
AGGAAAGTATTTCATATTTGAAGAGTATGGAATTTATTTTGTAAATGAAT	5	0.125	No Hit
ATATGACTCAAAAATTACAAGTGTTTATAACCCTACTTAGTTTAGTCATT	5	0.125	No Hit
AGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTC	5	0.125	No Hit
AGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	5	0.125	No Hit
AAGGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTT	5	0.125	No Hit
AGTTGCAATAAAATAAAAAAAAAATCTACAAAATTCAAATATATAAAATT	5	0.125	No Hit
ACCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	5	0.125	No Hit
TACCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCAT	5	0.125	No Hit
TGGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGAT	5	0.125	No Hit
ACTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATAT	5	0.125	No Hit
TAACCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCA	5	0.125	No Hit
ACTTTAAGTGGGGCCTAAATGAACTGTTGGACATCAGGTTCATGAAAACG	5	0.125	No Hit
TGTTGGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATA	5	0.125	No Hit
ACTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCA	5	0.125	No Hit
ACGATGTTTATTTTTATGATAAAAAGGATATACTTTTGTATTTTGGGGTA	5	0.125	No Hit
ACACAGATTGATGATCTCTAGGAAAAAGTAAAACAAAAACAAAACCTTCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.1	0.0
74-75	0.0	0.0	0.0	0.1375	0.0
76-77	0.0	0.0	0.0	0.175	0.0
78-79	0.0	0.0	0.0	0.225	0.0
80-81	0.0	0.0	0.0	0.375	0.0
82-83	0.0	0.0	0.0	0.6125	0.0
84-85	0.0	0.0	0.0	0.7125	0.0
86-87	0.0	0.0	0.0	0.8875	0.0
88-89	0.0	0.0	0.0	1.075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGTC	15	0.009957196	47.5	92-93
GTTTATA	35	0.00791152	27.142855	74-75
>>END_MODULE
SRR32569740 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569740_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.115	12.0	12.0	26.0	12.0	34.0
2	32.099	34.0	34.0	34.0	26.0	34.0
3	32.2315	34.0	34.0	34.0	26.0	34.0
4	31.6605	34.0	34.0	34.0	26.0	34.0
5	32.1625	34.0	34.0	34.0	26.0	34.0
6	31.8085	34.0	34.0	34.0	26.0	34.0
7	31.8725	34.0	34.0	34.0	26.0	34.0
8	32.2515	34.0	34.0	34.0	26.0	34.0
9	32.2945	34.0	34.0	34.0	26.0	34.0
10-11	32.241	34.0	34.0	34.0	26.0	34.0
12-13	32.219750000000005	34.0	34.0	34.0	26.0	34.0
14-15	32.348749999999995	34.0	34.0	34.0	26.0	34.0
16-17	32.4	34.0	34.0	34.0	26.0	34.0
18-19	32.18875	34.0	34.0	34.0	26.0	34.0
20-21	32.2485	34.0	34.0	34.0	26.0	34.0
22-23	32.211	34.0	34.0	34.0	26.0	34.0
24-25	32.03675	34.0	34.0	34.0	26.0	34.0
26-27	32.343500000000006	34.0	34.0	34.0	26.0	34.0
28-29	32.36925	34.0	34.0	34.0	26.0	34.0
30-31	32.357	34.0	34.0	34.0	26.0	34.0
32-33	32.323	34.0	34.0	34.0	26.0	34.0
34-35	31.988	34.0	34.0	34.0	26.0	34.0
36-37	32.347	34.0	34.0	34.0	26.0	34.0
38-39	32.3765	34.0	34.0	34.0	26.0	34.0
40-41	32.2145	34.0	34.0	34.0	26.0	34.0
42-43	32.195750000000004	34.0	34.0	34.0	26.0	34.0
44-45	32.24975	34.0	34.0	34.0	26.0	34.0
46-47	32.091499999999996	34.0	34.0	34.0	26.0	34.0
48-49	32.087999999999994	34.0	34.0	34.0	26.0	34.0
50-51	32.069	34.0	34.0	34.0	26.0	34.0
52-53	32.04675	34.0	34.0	34.0	26.0	34.0
54-55	31.905	34.0	34.0	34.0	26.0	34.0
56-57	32.03725	34.0	34.0	34.0	26.0	34.0
58-59	31.88375	34.0	34.0	34.0	26.0	34.0
60-61	31.8845	34.0	34.0	34.0	26.0	34.0
62-63	31.945999999999998	34.0	34.0	34.0	26.0	34.0
64-65	31.891750000000002	34.0	34.0	34.0	26.0	34.0
66-67	31.746000000000002	34.0	34.0	34.0	26.0	34.0
68-69	31.6715	34.0	34.0	34.0	26.0	34.0
70-71	31.6215	34.0	34.0	34.0	26.0	34.0
72-73	31.6645	34.0	34.0	34.0	26.0	34.0
74-75	31.51575	34.0	34.0	34.0	26.0	34.0
76-77	31.59975	34.0	34.0	34.0	26.0	34.0
78-79	31.30075	34.0	34.0	34.0	26.0	34.0
80-81	31.6385	34.0	34.0	34.0	26.0	34.0
82-83	31.520249999999997	34.0	34.0	34.0	26.0	34.0
84-85	31.44175	34.0	34.0	34.0	26.0	34.0
86-87	31.31725	34.0	34.0	34.0	26.0	34.0
88-89	31.29775	34.0	34.0	34.0	26.0	34.0
90-91	31.18	34.0	34.0	34.0	19.0	34.0
92-93	31.084	34.0	34.0	34.0	12.0	34.0
94-95	31.1405	34.0	34.0	34.0	19.0	34.0
96-97	30.753999999999998	34.0	34.0	34.0	12.0	34.0
98-99	30.84825	34.0	34.0	34.0	12.0	34.0
100-101	30.665	34.0	34.0	34.0	12.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	2.0
23	6.0
24	15.0
25	22.0
26	64.0
27	91.0
28	136.0
29	225.0
30	412.0
31	741.0
32	1326.0
33	955.0
34	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.125	28.325	33.825	13.725000000000001
2	27.32981662898769	30.293896006028636	18.136146696809845	24.240140668173826
3	23.65	38.3	27.075	10.975
4	19.400000000000002	33.425	30.425	16.75
5	17.95	40.45	24.15	17.45
6	20.225	40.175	24.575	15.024999999999999
7	17.575	42.15	22.8	17.474999999999998
8	14.924999999999999	20.525	45.275	19.275000000000002
9	17.4	31.874999999999996	28.000000000000004	22.725
10-11	18.825	33.8125	29.65	17.712500000000002
12-13	19.125	30.1875	27.200000000000003	23.4875
14-15	17.549999999999997	30.675	31.912499999999998	19.8625
16-17	17.125	31.5625	30.525000000000002	20.7875
18-19	18.55	34.4375	27.900000000000002	19.112499999999997
20-21	17.849999999999998	33.6875	30.375000000000004	18.087500000000002
22-23	18.85	32.5125	28.375	20.2625
24-25	17.0375	34.5125	29.225	19.225
26-27	17.5625	33.35	29.062500000000004	20.025000000000002
28-29	18.637500000000003	31.825	30.062499999999996	19.475
30-31	17.974999999999998	32.05	30.6875	19.287499999999998
32-33	17.0375	33.1875	31.337500000000002	18.4375
34-35	18.558262845600304	30.980936750410304	33.581618482514834	16.87918192147456
36-37	19.75	30.95	31.2375	18.0625
38-39	17.875	30.7	32.175	19.25
40-41	18.587500000000002	32.824999999999996	30.1375	18.45
42-43	16.175	30.425	31.7375	21.6625
44-45	17.5125	32.3625	30.5125	19.6125
46-47	17.2	31.2875	30.625000000000004	20.8875
48-49	16.675	31.025000000000002	32.9875	19.3125
50-51	16.325	30.9375	33.074999999999996	19.662499999999998
52-53	18.8	31.637500000000003	30.55	19.0125
54-55	18.3125	32.5125	29.362500000000004	19.8125
56-57	16.5625	34.125	29.425	19.8875
58-59	17.1125	31.2625	31.2875	20.3375
60-61	18.825	32.975	29.7375	18.462500000000002
62-63	17.6875	31.2875	31.374999999999996	19.650000000000002
64-65	18.1375	32.8625	30.4875	18.512500000000003
66-67	18.8875	31.612499999999997	30.0375	19.4625
68-69	17.6875	32.9375	31.225	18.15
70-71	17.75	31.3125	32.125	18.8125
72-73	18.2875	32.525	31.2875	17.9
74-75	17.575	31.7	32.525	18.2
76-77	16.537499999999998	31.837500000000002	31.65	19.975
78-79	16.954670673678937	31.88079138492362	31.016779363886805	20.147758577510643
80-81	17.5125	31.2	33.225	18.0625
82-83	18.8	29.812499999999996	33.5125	17.875
84-85	16.5875	31.7625	31.924999999999997	19.725
86-87	15.675	35.05	31.6	17.675
88-89	17.9875	30.9	32.4625	18.65
90-91	17.1625	31.825	31.112499999999997	19.900000000000002
92-93	18.3	32.9	30.65	18.15
94-95	19.475	33.3125	28.6375	18.575
96-97	17.076554420481777	32.22348341531088	30.634380123596923	20.065582040610416
98-99	16.325	32.3125	32.475	18.8875
100-101	18.675	30.599999999999998	31.374999999999996	19.35
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	4.5
19	5.0
20	5.0
21	6.5
22	7.5
23	7.0
24	20.5
25	41.5
26	66.0
27	83.5
28	98.5
29	135.0
30	182.0
31	203.0
32	194.0
33	195.5
34	191.5
35	196.0
36	208.0
37	184.0
38	153.5
39	163.0
40	170.0
41	183.0
42	224.5
43	224.0
44	197.0
45	160.0
46	107.5
47	80.5
48	70.5
49	59.5
50	49.5
51	43.0
52	35.0
53	20.5
54	11.0
55	4.0
56	2.0
57	0.5
58	0.0
59	0.0
60	0.5
61	0.5
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.5
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.475
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.9875
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.17500000000000002
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.8875
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59307359307358	51.0
2	15.836940836940839	21.95
3	6.349206349206349	13.200000000000001
4	2.417027417027417	6.7
5	0.974025974025974	3.375
6	0.468975468975469	1.95
7	0.25252525252525254	1.225
8	0.10822510822510822	0.6
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGAGAACCGTGTGCATCACAGGAAGAAGAAGGGCGGATGCCACCATTG	8	0.2	No Hit
ACAGCAATCATACCCAGAAAAAGAGCTGTTTCTTCTATTATCTCTGATTC	8	0.2	No Hit
ACATCATTCTGTAAGCATCATATATGTGATTATTTGTAAATACAACTGCC	8	0.2	No Hit
TCATCATTCTGTAAGCATCATATATGTGATTATTTGTAAATACAACTGCC	7	0.17500000000000002	No Hit
TGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCAC	7	0.17500000000000002	No Hit
TAGCTGAACAGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAGAGA	7	0.17500000000000002	No Hit
GAGAATGTGGAATGGTGTTTCTGCTCAGAACTCTTGAGCTGATTCATCTC	7	0.17500000000000002	No Hit
TAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAG	7	0.17500000000000002	No Hit
TTGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAAC	7	0.17500000000000002	No Hit
TTGGAGAACCGTGTGCATCACAGGAAGAAGAAGGGCGGATGCCACCATTG	7	0.17500000000000002	No Hit
AAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAG	6	0.15	No Hit
ATGAGCTGATTCATCTCCATATTATAAATGTATTTTAAATGTTTCAGTGA	6	0.15	No Hit
AATTCCATCCAGGTATAGTGACAAGAGGGTATGTGGTTCAATGATAGCAA	6	0.15	No Hit
AGGAGAACCGTGTGCATCACAGGAAGAAGAAGGGCGGATGCCACCATTGT	6	0.15	No Hit
TGAAACCAATATTTTTTCTACCCCAAAATACAAAAGTATATCCTTTTTAT	6	0.15	No Hit
AGGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGT	6	0.15	No Hit
TCTCAAGAATAAATGCAGAGGGACAGGTATGAAGATGGCTTCCTATTCTC	6	0.15	No Hit
TGGCAAATGAAAGACACAGACACAAAGAGAAAAATCTTCTACTTCCTAAA	6	0.15	No Hit
TGTTTGGTTTCAAAATACAAAACCATACACTTCACAATTAACAAATTATT	6	0.15	No Hit
GAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAAATCTAA	6	0.15	No Hit
TCAGCAATCATACCCAGAAAAAGAGCTGTTTCTTCTATTATCTCTGATTC	6	0.15	No Hit
TCCTGCTTCCAGATGGCAAGAGTCTGGGCTCCTTACATTGGTTCTCCATT	6	0.15	No Hit
ACTCAAAAATATCTACAGTGCTAGAATAGAGTTTGTTAGGATAGTCAGGT	6	0.15	No Hit
TGTGCTTTTCTCCTTCCTCTTTCCAATGTTTGTATTTTAAATTTATCTCT	5	0.125	No Hit
AAGCTGAACAGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAGAGA	5	0.125	No Hit
ACACAGACTGTGGCCACAAACCAGGTACTTATGATGCTTCAAAAGCAGCT	5	0.125	No Hit
ATCATTCTGTAAGCATCATATATGTGATTATTTGTAAATACAACTGCCAA	5	0.125	No Hit
TAAACCATCATACTTGGTGAGCCTGCTTCCAGATGGCAAGAGTCTGGGCT	5	0.125	No Hit
GGGTGTGTGCAGGCAACGTGAAGATATATCACCCGGACATCACGGTGCCT	5	0.125	No Hit
TGGTGTGTGCAGGCAACGTGAAGATATATCACCCGGACATCACGGTGCCT	5	0.125	No Hit
TCCCAACTCACACCAGACTTGCTTTATTTGAATTTTTTAAAGCATGGTTT	5	0.125	No Hit
TACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATTCTGATGCA	5	0.125	No Hit
TCAGTAAGTGGGTAAAAGGTAATTTGAATATTCCTGACTTTTTATCTCCT	5	0.125	No Hit
GGTTTGGTTTCAAAATACAAAACCATACACTTCACAATTAACAAATTATT	5	0.125	No Hit
TAAGCAACCCAACTCACACCAGACTTGCTTTATTTGAATTTTTTAAAGCA	5	0.125	No Hit
AGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCAC	5	0.125	No Hit
GTAGTATTCTGATGCATAGTCGGTGACATTCTTGAGTTTCTCTCTCCATT	5	0.125	No Hit
TCGACATTAGACTTTCTGAAGCATGACTGTAGCATAGAAAATTATGTGAC	5	0.125	No Hit
GGGTAATTTGAATATTCCTGACTTTTTATCTCCTTCTGAAACCAATATTT	5	0.125	No Hit
AAACATAATGTGGCAGATGTTGAAATTCATTCTTGAGTCTCTCCTTTCCC	5	0.125	No Hit
AAAGACACAGACACAAAGAGAAAAATCTTCTACTTCCTAAAAGAACTATT	5	0.125	No Hit
GTGCAGAGGGACAGGTATGAAGATGGCTTCCTATTCTCAGTAAGGAGAGA	5	0.125	No Hit
AACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATTCTGATGCA	5	0.125	No Hit
CGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACTA	5	0.125	No Hit
TCATAACCAGACCTTCCTTTAGAATTCAACTTGTAAGTGTGTCTTTCTTG	5	0.125	No Hit
TAGATTCTATCACAAAGTGAAAGATAAAATCTAAATAGGTTAGAGAGAGC	5	0.125	No Hit
TAAGACACAGACACAAAGAGAAAAATCTTCTACTTCCTAAAAGAACTATT	5	0.125	No Hit
AAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAAATCTAA	5	0.125	No Hit
ACGGTGCCTGGCCTCGGATGCAAACCTGCCAGTCCTGTCTCTTACTTTGT	5	0.125	No Hit
TCACCTGGGTCCCCATCCCTACTCTATCTCTATACCTCAAAACTTACTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.1	0.0
74-75	0.0	0.0	0.0	0.1375	0.0
76-77	0.0	0.0	0.0	0.175	0.0
78-79	0.0	0.0	0.0	0.225	0.0
80-81	0.0	0.0	0.0	0.4125	0.0
82-83	0.0	0.0	0.0	0.625	0.0
84-85	0.0	0.0	0.0	0.7125	0.0
86-87	0.0	0.0	0.0	0.8875	0.0
88-89	0.0	0.0	0.0	1.075	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297745 spots for SRR32569740.sra
Written 10297745 spots for SRR32569740.sra
Read 10297752 spots for SRR32569740.sra
Written 10297752 spots for SRR32569740.sra
SRR ids: ['SRR32569740.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_jv8ub4sv
SRR32569740.sra spots: 82381967
blocks: [[1, 10297745], [10297746, 20595490], [20595491, 30893235], [30893236, 41190980], [41190981, 51488725], [51488726, 61786470], [61786471, 72084215], [72084216, 82381967]]
SRR32569740 file size 19930200
SRR32569740 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32569740 SRR32569740_1.fastq SRR32569740_2.fastq
Input file:	SRR32569740_1.fastq
Paired file:	SRR32569740_2.fastq
trimmed:	SRR32569740-trimmed-pair1.fastq, SRR32569740-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 04:35:00 2026 >> started

Tue Mar 31 04:37:52 2026 >> done (171.690s)
82381967 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
82381967 (100.00%) read pairs available; of these:
    6212 ( 0.01%) trimmed read pairs available after processing
82375755 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 93	       1	  0.00%
 94	       1	  0.00%
 95	       0	  0.00%
 96	       2	  0.00%
 97	       4	  0.00%
 98	      83	  0.00%
 99	     305	  0.00%
100	    5816	  0.01%
101	82375755	 99.99%
82381967 reads passed initial QC


criterion=sequence-density
sequence-density=9.18
sequence-density-rank=1
fanout-score=2.10
fanout-score-rank=10
prefix-density=9.94
prefix-fanout=1.9
sequence=TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCAT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=41
fanout-score=134.77
fanout-score-rank=1
prefix-density=3.47
prefix-fanout=1.0
sequence=AATGCGTAGAGTGGGGAGAGCAA


criterion=sequence-density
sequence-density=11.94
sequence-density-rank=1
fanout-score=2.01
fanout-score-rank=24
prefix-density=11.87
prefix-fanout=2.0
sequence=CTAATAATTGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=827.48
fanout-score-rank=1
prefix-density=7.26
prefix-fanout=1.1
sequence=TAAAAGTCTCAACACAAACATTC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCAT -y CTAATAATTGTTT -o SRR32569740 SRR32569740_1.fastq SRR32569740_2.fastq
Input file:	SRR32569740_1.fastq
Paired file:	SRR32569740_2.fastq
trimmed:	SRR32569740-trimmed-pair1.fastq, SRR32569740-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTG
-- paired 3' end adapter sequence (-y):	CTAATAATTGTTT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 04:43:07 2026 >> started

Tue Mar 31 04:44:34 2026 >> done (86.249s)
67403428 read pairs processed; of these:
     871 ( 0.00%) short read pairs filtered out after trimming by size control
    1396 ( 0.00%) empty read pairs filtered out after trimming by size control
67401161 (100.00%) read pairs available; of these:
  304127 ( 0.45%) trimmed read pairs available after processing
67097034 (99.55%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 26	       1	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	       0	  0.00%
 52	       0	  0.00%
 53	       0	  0.00%
 54	       0	  0.00%
 55	       0	  0.00%
 56	       0	  0.00%
 57	       0	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       0	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	       0	  0.00%
 68	       0	  0.00%
 69	       0	  0.00%
 70	       0	  0.00%
 71	       0	  0.00%
 72	       0	  0.00%
 73	       0	  0.00%
 74	       0	  0.00%
 75	       0	  0.00%
 76	       0	  0.00%
 77	       0	  0.00%
 78	       0	  0.00%
 79	       0	  0.00%
 80	       0	  0.00%
 81	       0	  0.00%
 82	       0	  0.00%
 83	       0	  0.00%
 84	       0	  0.00%
 85	       0	  0.00%
 86	       0	  0.00%
 87	       0	  0.00%
 88	       0	  0.00%
 89	       0	  0.00%
 90	       0	  0.00%
 91	       0	  0.00%
 92	       0	  0.00%
 93	       1	  0.00%
 94	       0	  0.00%
 95	       0	  0.00%
 96	       0	  0.00%
 97	       6	  0.00%
 98	     121	  0.00%
 99	  150217	  0.22%
100	  158637	  0.24%
101	67092178	 99.54%


criterion=sequence-density
sequence-density=1.33
sequence-density-rank=1
fanout-score=54.66
fanout-score-rank=2
prefix-density=1.66
prefix-fanout=43.8
sequence=ACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGAGGCAACCATCTCG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=21
fanout-score=78.38
fanout-score-rank=1
prefix-density=0.26
prefix-fanout=9.5
sequence=CTCCACCTCCAGGGTGATGGTCTTGCC


criterion=sequence-density
sequence-density=1.36
sequence-density-rank=1
fanout-score=38.13
fanout-score-rank=8
prefix-density=1.73
prefix-fanout=30.0
sequence=ACTGTCTCTTATACACATCTGACGCTGCCGACGACCATCATTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=8
fanout-score=199.37
fanout-score-rank=1
prefix-density=0.74
prefix-fanout=22.3
sequence=CACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAG
SRR32569740 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 04:47:18
                             Started mapping on |	Mar 31 04:47:18
                                    Finished on |	Mar 31 04:55:34
       Mapping speed, Million of reads per hour |	597.92

                          Number of input reads |	82379700
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	74999094
                        Uniquely mapped reads % |	91.04%
                          Average mapped length |	198.61
                       Number of splices: Total |	43185663
            Number of splices: Annotated (sjdb) |	42756841
                       Number of splices: GT/AG |	42717925
                       Number of splices: GC/AG |	355717
                       Number of splices: AT/AC |	47777
               Number of splices: Non-canonical |	64244
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.87
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.28
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4120701
             % of reads mapped to multiple loci |	5.00%
        Number of reads mapped to too many loci |	129515
             % of reads mapped to too many loci |	0.16%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.78%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3259905	3259905	3259905
N_multimapping	4120701	4120701	4120701
N_noFeature	4819852	72094638	5322577
N_ambiguous	3359489	153887	989706
UnstrandedReadsAssigned:66819753 PositiveStrandReadsAssigned:2750569 NegativeStrandReadsAssigned:68686811
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32569740 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32569740-trimmed-pair1.fastq
                             SRR32569740-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 82,379,700 reads, 72,695,919 reads pseudoaligned
[quant] estimated average fragment length: 199.662
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,196 rounds

 109283 SRR32569740.ke.tsv
  52636 SRR32569740.se.tsv
 161919 total
==> SRR32569740.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	7	0	0
ENSMUST00000196221.1	9	4.5	0	0
ENSMUST00000179664.1	11	6.5	0	0
ENSMUST00000178537.1	12	7.5	0	0
ENSMUST00000178862.1	14	7	0	0
ENSMUST00000179520.1	11	6.5	0	0
ENSMUST00000179883.1	16	7	0	0
ENSMUST00000195858.1	10	5.5	0	0
ENSMUST00000179932.1	12	7.5	0	0

==> SRR32569740.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	3121
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32569740 completed mapping pipeline successfully
