Starting /dee2/code/volunteer_pipeline.sh SRR32569737
    current disk space = 789443752
    free memory = 194346656 
SRR32569737 SRAfilesize
ad64401f0fa55451bc0db46c87ef4956  SRR32569737.sra
SRR32569737.sra file validated
SRR32569737 is paired end
SRR32569737 is conventional basespace
SRR32569737 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.072	26.0	12.0	34.0	12.0	34.0
2	32.8215	34.0	34.0	34.0	34.0	34.0
3	33.188	34.0	34.0	34.0	34.0	34.0
4	33.1425	34.0	34.0	34.0	34.0	34.0
5	33.3145	34.0	34.0	34.0	34.0	34.0
6	33.0805	34.0	34.0	34.0	34.0	34.0
7	33.0515	34.0	34.0	34.0	34.0	34.0
8	33.1545	34.0	34.0	34.0	34.0	34.0
9	33.0895	34.0	34.0	34.0	34.0	34.0
10-11	33.11425	34.0	34.0	34.0	34.0	34.0
12-13	33.0835	34.0	34.0	34.0	34.0	34.0
14-15	33.07725	34.0	34.0	34.0	34.0	34.0
16-17	33.047	34.0	34.0	34.0	34.0	34.0
18-19	33.1025	34.0	34.0	34.0	34.0	34.0
20-21	33.04	34.0	34.0	34.0	34.0	34.0
22-23	32.956	34.0	34.0	34.0	34.0	34.0
24-25	33.01675	34.0	34.0	34.0	34.0	34.0
26-27	33.073	34.0	34.0	34.0	34.0	34.0
28-29	32.971000000000004	34.0	34.0	34.0	34.0	34.0
30-31	33.0975	34.0	34.0	34.0	34.0	34.0
32-33	32.963499999999996	34.0	34.0	34.0	34.0	34.0
34-35	33.03875	34.0	34.0	34.0	34.0	34.0
36-37	32.922	34.0	34.0	34.0	34.0	34.0
38-39	32.89875	34.0	34.0	34.0	34.0	34.0
40-41	32.8565	34.0	34.0	34.0	34.0	34.0
42-43	32.92775	34.0	34.0	34.0	34.0	34.0
44-45	32.816	34.0	34.0	34.0	34.0	34.0
46-47	32.826	34.0	34.0	34.0	34.0	34.0
48-49	32.81275	34.0	34.0	34.0	34.0	34.0
50-51	32.666	34.0	34.0	34.0	30.0	34.0
52-53	32.56125	34.0	34.0	34.0	30.0	34.0
54-55	32.61625	34.0	34.0	34.0	30.0	34.0
56-57	32.704499999999996	34.0	34.0	34.0	34.0	34.0
58-59	32.689	34.0	34.0	34.0	34.0	34.0
60-61	32.59	34.0	34.0	34.0	30.0	34.0
62-63	32.638000000000005	34.0	34.0	34.0	34.0	34.0
64-65	32.55375	34.0	34.0	34.0	30.0	34.0
66-67	32.50425	34.0	34.0	34.0	26.0	34.0
68-69	32.552499999999995	34.0	34.0	34.0	26.0	34.0
70-71	32.48350000000001	34.0	34.0	34.0	30.0	34.0
72-73	32.3755	34.0	34.0	34.0	26.0	34.0
74-75	32.29925	34.0	34.0	34.0	26.0	34.0
76-77	32.2655	34.0	34.0	34.0	26.0	34.0
78-79	32.31525	34.0	34.0	34.0	26.0	34.0
80-81	32.333749999999995	34.0	34.0	34.0	26.0	34.0
82-83	32.234	34.0	34.0	34.0	26.0	34.0
84-85	32.28425	34.0	34.0	34.0	26.0	34.0
86-87	32.21	34.0	34.0	34.0	26.0	34.0
88-89	32.325	34.0	34.0	34.0	26.0	34.0
90-91	32.11175	34.0	34.0	34.0	26.0	34.0
92-93	32.1255	34.0	34.0	34.0	26.0	34.0
94-95	31.91725	34.0	34.0	34.0	26.0	34.0
96-97	32.09375	34.0	34.0	34.0	26.0	34.0
98-99	31.9815	34.0	34.0	34.0	26.0	34.0
100-101	31.812	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	5.0
25	10.0
26	27.0
27	32.0
28	81.0
29	119.0
30	227.0
31	336.0
32	1069.0
33	2063.0
34	31.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	0.8999999999999999	54.074999999999996	43.925	1.0999999999999999
2	40.400000000000006	12.7	12.475	34.425
3	22.75	16.625	34.449999999999996	26.174999999999997
4	22.625	22.125	26.6	28.65
5	23.65	30.65	24.9	20.8
6	25.575	33.6	24.875	15.950000000000001
7	24.725	31.7	28.449999999999996	15.125
8	17.65	26.325	44.0	12.025
9	19.675	28.799999999999997	36.675000000000004	14.85
10-11	17.125	32.625	37.1125	13.1375
12-13	21.2625	27.537499999999998	31.937500000000004	19.2625
14-15	17.7875	30.125	34.7875	17.299999999999997
16-17	19.287499999999998	28.012500000000003	35.9375	16.7625
18-19	22.525000000000002	27.1125	33.15	17.2125
20-21	21.0375	30.9	31.3	16.7625
22-23	20.474999999999998	30.775000000000002	31.2625	17.4875
24-25	20.2125	30.175	31.324999999999996	18.2875
26-27	20.837500000000002	30.2375	31.162499999999998	17.7625
28-29	17.6375	31.275	31.362499999999997	19.725
30-31	18.25	30.4625	31.724999999999998	19.5625
32-33	16.45	30.675	32.5625	20.3125
34-35	17.0	31.324999999999996	32.4125	19.2625
36-37	16.662499999999998	31.0625	33.3625	18.912499999999998
38-39	16.775000000000002	31.724999999999998	34.2875	17.2125
40-41	19.875	29.2	32.300000000000004	18.625
42-43	21.349999999999998	29.2375	31.05	18.3625
44-45	18.025	34.4	28.799999999999997	18.775
46-47	19.45	33.0875	30.5375	16.925
48-49	20.05	32.2875	30.075000000000003	17.5875
50-51	19.5	32.3125	29.912499999999998	18.275
52-53	19.25	29.425	31.2375	20.0875
54-55	18.75	32.7	31.275	17.275
56-57	18.4125	34.2625	29.799999999999997	17.525
58-59	16.650000000000002	29.8875	34.25	19.2125
60-61	16.55	33.650000000000006	28.8375	20.962500000000002
62-63	17.75	31.362499999999997	31.4375	19.45
64-65	19.287499999999998	30.875000000000004	32.35	17.4875
66-67	18.75	32.0625	32.300000000000004	16.8875
68-69	19.0625	32.25	30.0375	18.65
70-71	18.462500000000002	31.837500000000002	31.637500000000003	18.0625
72-73	16.2125	31.8	30.7875	21.2
74-75	19.025	32.725	29.9375	18.3125
76-77	18.212500000000002	31.424999999999997	33.775	16.5875
78-79	17.2625	32.300000000000004	33.775	16.662499999999998
80-81	17.4125	32.875	33.300000000000004	16.412499999999998
82-83	19.075	31.974999999999998	32.2	16.75
84-85	18.862499999999997	30.662499999999998	32.4	18.075
86-87	18.95	30.2875	32.95	17.8125
88-89	19.475	29.575000000000003	34.35	16.6
90-91	19.0125	32.7625	29.7	18.525
92-93	18.9375	33.4375	29.849999999999998	17.775
94-95	17.9125	32.800000000000004	31.2625	18.025
96-97	18.575	29.975	33.35	18.099999999999998
98-99	17.7	32.625	30.7375	18.9375
100-101	18.825	30.8125	31.7375	18.625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	1.5
17	16.5
18	20.0
19	7.5
20	21.0
21	31.0
22	30.5
23	22.0
24	10.0
25	16.5
26	30.5
27	50.0
28	65.5
29	100.5
30	176.5
31	251.0
32	252.0
33	214.0
34	192.0
35	174.5
36	152.5
37	122.0
38	140.0
39	200.0
40	160.0
41	169.5
42	244.5
43	222.0
44	186.0
45	156.0
46	113.0
47	80.0
48	69.0
49	78.0
50	86.5
51	66.5
52	36.5
53	16.0
54	8.0
55	2.0
56	0.5
57	1.0
58	1.0
59	0.5
60	0.0
61	2.5
62	2.5
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.5147347740668	29.275000000000002
2	19.842829076620824	20.200000000000003
3	10.609037328094303	16.2
4	4.6660117878192535	9.5
5	3.2907662082514735	8.375
6	1.424361493123772	4.35
7	1.031434184675835	3.675
8	0.6385068762278978	2.6
9	0.19646365422396855	0.8999999999999999
>10	0.7858546168958742	4.925
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	21	0.525	No Hit
AGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	15	0.375	No Hit
AGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	15	0.375	No Hit
AGCTGAGGTAGTTAAATGTGAAACTTACACAACTTAAAATAATGAGAGAA	13	0.325	No Hit
AGGGGCCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTG	13	0.325	No Hit
TGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	12	0.3	No Hit
AGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATT	12	0.3	No Hit
AGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	12	0.3	No Hit
AGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	12	0.3	No Hit
TCTCTATAGAATGTTCTCTCCACCACCTCCTGCTAGTCATACCAAACCCA	11	0.27499999999999997	No Hit
ACCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	11	0.27499999999999997	No Hit
TCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	10	0.25	No Hit
TGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	10	0.25	No Hit
AGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	10	0.25	No Hit
AGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTC	10	0.25	No Hit
AGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	10	0.25	No Hit
AGTCCATATGAGGGATTAATTGTTCTCATTATAAAGTTTATAATTTCTGC	9	0.22499999999999998	No Hit
ACAAGGAAATAGACATTGTTTTGAAATAGTTGTACAGGATCTTCGCTGAG	9	0.22499999999999998	No Hit
ACCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	9	0.22499999999999998	No Hit
TCCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	9	0.22499999999999998	No Hit
ATGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTTCCTGTGATGCAC	8	0.2	No Hit
ATACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGG	8	0.2	No Hit
TGCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCAC	8	0.2	No Hit
AGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAGAA	8	0.2	No Hit
TGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	8	0.2	No Hit
TCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTG	8	0.2	No Hit
AGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	8	0.2	No Hit
TCAAGGAAATAGACATTGTTTTGAAATAGTTGTACAGGATCTTCGCTGAG	8	0.2	No Hit
AGTTGCAAAGATGTTGTGTTAGAAATGGTATAAAAACCCAGTTAAACCAT	8	0.2	No Hit
TATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATATGA	8	0.2	No Hit
TCTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATA	8	0.2	No Hit
TGTTGCAAAGATGTTGTGTTAGAAATGGTATAAAAACCCAGTTAAACCAT	8	0.2	No Hit
ATCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGC	8	0.2	No Hit
TCCACCACCTCCTGCTAGTCATACCAAACCCACCCAAAACTAGACCAAAT	7	0.17500000000000002	No Hit
TTGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCCATCTGGCTACAGAG	7	0.17500000000000002	No Hit
ACTCCACTGCGCCCCATTGATCAGACAATGGCTTTAAGTGGGGCCTAAAT	7	0.17500000000000002	No Hit
AGTGTCAATACTGTAAGTATTCCAAGCACAACTTAATTTCTAATTCACTC	7	0.17500000000000002	No Hit
TATTTCATATCAGTCAACCCAGAATCAGAGATAATAGAAGAAACAGCTCT	7	0.17500000000000002	No Hit
AGGGGAGACTTCAAATGAAATATTGTATTTGAACAATAAAAAGTTAAAGA	7	0.17500000000000002	No Hit
TGCTGAGGTAGTTAAATGTGAAACTTACACAACTTAAAATAATGAGAGAA	7	0.17500000000000002	No Hit
TCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	7	0.17500000000000002	No Hit
AGGCTTTGGTGTACAGGGAAAGGAGAGACTCAAGAATGAATTTCAACATC	7	0.17500000000000002	No Hit
TCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCATAAAATT	7	0.17500000000000002	No Hit
TGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAGAA	7	0.17500000000000002	No Hit
TGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	7	0.17500000000000002	No Hit
AGCAAGATTCTCATTAAGGCTAACTATGCTATTCTCCACTGCGCCCCATT	7	0.17500000000000002	No Hit
TCAGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAG	7	0.17500000000000002	No Hit
AGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTA	7	0.17500000000000002	No Hit
TCCCAGAAGAGGCAGTGGAAGGCCTCGAAGAACCATCACATTGTATAAGG	7	0.17500000000000002	No Hit
TGGGGGAAAAAATGAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAG	7	0.17500000000000002	No Hit
ACTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATATGA	7	0.17500000000000002	No Hit
AGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAA	7	0.17500000000000002	No Hit
AGTTCTTTTGGATGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTT	7	0.17500000000000002	No Hit
ACTGGCGGTCTCTAACCCAAGTGCTTTACCAGCTTTCACATTCCTTATAA	7	0.17500000000000002	No Hit
AGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	6	0.15	No Hit
ACAGTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAA	6	0.15	No Hit
TCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	6	0.15	No Hit
TCTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	6	0.15	No Hit
ACGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCA	6	0.15	No Hit
TGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGGT	6	0.15	No Hit
ACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATA	6	0.15	No Hit
AGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	6	0.15	No Hit
AGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTGTGT	6	0.15	No Hit
AGTGTATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCAT	6	0.15	No Hit
TGTTGACATAAATAAGCTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTT	6	0.15	No Hit
ATGGCATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTT	6	0.15	No Hit
AGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCAT	6	0.15	No Hit
AGCTAACTATGCTATTCTCCACTGCGCCCCATTGATCAGACAATGGCTTT	6	0.15	No Hit
AGCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCAC	6	0.15	No Hit
ACTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATACA	6	0.15	No Hit
TGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	6	0.15	No Hit
TATTATTAGAAATTCCAACCCTAGTTATGAAAGATTGAGGGAAAGTGATA	6	0.15	No Hit
TGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	6	0.15	No Hit
ACATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGC	6	0.15	No Hit
TGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGC	6	0.15	No Hit
AGTTTGATTTGGGCAAAGTTGTCTTTTTCCTCAATCTGCAGCCATAAAAT	6	0.15	No Hit
TGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCATAA	6	0.15	No Hit
ACAGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAG	6	0.15	No Hit
AGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAAAGCCTTTGTGG	6	0.15	No Hit
ACTTAGGGATTAGAAAGGGCCAGGTTGTCTAGATGTATTTATAAACATTT	6	0.15	No Hit
TGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTA	6	0.15	No Hit
AGCACAGATTGATGATCTCTAGGAAAAAGTAAAACAAAAACAAAACCTTC	6	0.15	No Hit
TCTGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAA	6	0.15	No Hit
TCCTGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGA	5	0.125	No Hit
AGCCATCTTCATACCTGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCC	5	0.125	No Hit
TGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	5	0.125	No Hit
TCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	5	0.125	No Hit
TGGGGCCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTG	5	0.125	No Hit
AATATGGTTGACATAAATAAGCTGGGAAATAGTTCTTTTAGGAAGTAGAA	5	0.125	No Hit
AGGAAAGGAGAGACTCAAGAATGAATTTCAACATCTGCCACATTATGTTA	5	0.125	No Hit
ACCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	5	0.125	No Hit
TGTCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGG	5	0.125	No Hit
AGCCATCTGGAAGCAGGCTCACCAAGTATGATGGTTTCATACCCAGAAAA	5	0.125	No Hit
AATTTATTTTGTAAATGAATTTAGTTATTAAATAAGAGTACTGAATGGAG	5	0.125	No Hit
TGGCTTTGGTGTACAGGGAAAGGAGAGACTCAAGAATGAATTTCAACATC	5	0.125	No Hit
ACCATTGAGAATTCTAAGCCATTATGTGGAGGAGCTGTTCAGCTCTCTCT	5	0.125	No Hit
TCCTGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCCATCTGGCTACAG	5	0.125	No Hit
TCTCCTTTTGAAAGGTGAATTTCTTTCATGGTAGACTTTTTTCCACATGG	5	0.125	No Hit
AGTCACCGACTATGCATCAGAATACTATGTACAGTTGAAGTTAGGGAGCT	5	0.125	No Hit
AACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGTA	5	0.125	No Hit
TACTGAATGGAGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGT	5	0.125	No Hit
ACTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	5	0.125	No Hit
TCTCAGAGCAAGTTGTACAGCTAAAGTGCCCTTTTGGGGGAATTGGTCAC	5	0.125	No Hit
TACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAA	5	0.125	No Hit
AACCGACTATGCATCAGAATACTATGTACAGTTGAAGTTAGGGAGCTGAG	5	0.125	No Hit
AGTTAAATGTGAAACTTACACAACTTAAAATAATGAGAGAATGGAGAACC	5	0.125	No Hit
ACACCGACTATGCATCAGAATACTATGTACAGTTGAAGTTAGGGAGCTGA	5	0.125	No Hit
TGCACTCTATTTGAGTAGATCAACATCTTTGCCAGCCAGCAGACCAGAAA	5	0.125	No Hit
TGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	5	0.125	No Hit
ACTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTG	5	0.125	No Hit
TGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTC	5	0.125	No Hit
AGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGGT	5	0.125	No Hit
ATAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAACAACAGTAGCT	5	0.125	No Hit
AGCTGAATTGGGGACTCTACATGGCTCTGAAAAATGAGATCAGCCATACA	5	0.125	No Hit
TCTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	5	0.125	No Hit
ATGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAAT	5	0.125	No Hit
ACCTATTTATGCAAGTAGCCATATTTCAATATGGTGCTGAAGCCTTTGAA	5	0.125	No Hit
TGCTCTCTCTAACCTATTTAGATTTTATCTTTCACTTTGTGATAGAATCT	5	0.125	No Hit
AGTATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATAT	5	0.125	No Hit
TCTCATTTTTAACAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTT	5	0.125	No Hit
TGTTAAATGTGAAACTTACACAACTTAAAATAATGAGAGAATGGAGAACC	5	0.125	No Hit
TGGCAAATTAAGCAAATATGTACAGTCTCTTTGTGCCAGGAGGATGTGAC	5	0.125	No Hit
TCCAAGTATGATGGTTTCATACCCAGAAAAACATTTGTTCTTTTGGATGC	5	0.125	No Hit
AGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAA	5	0.125	No Hit
ACTCTCCACCACCTCCTGCTAGTCATACCAAACCCACCCAAAACTAGACC	5	0.125	No Hit
TAGATAGAGTAGGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTTCTT	5	0.125	No Hit
AGCCATACAAAGTCCTTAGGGATTAGAAAGGGCCAGGTTGTCTAGATGTA	5	0.125	No Hit
AGTCCAAAATAGTTAACGTACCAGGTAGACAAACACAGCTTAGTGATGAG	5	0.125	No Hit
ACTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGACT	5	0.125	No Hit
TCACTGAAAAGAGACTCAACTCTTTTTCCCCCCAGCAATATTATGTTGCT	5	0.125	No Hit
AGGAAGCCATCTTCATACCTGTCCCTCTGCATTTATTCTTGAGGTTACTG	5	0.125	No Hit
AGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTGG	5	0.125	No Hit
TGCCATCTTCATACCTGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCC	5	0.125	No Hit
AGCACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAGCAG	5	0.125	No Hit
AATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATATGA	5	0.125	No Hit
TCCAAGATCTCTACAGTAAGTGCCTCAGAGCAAGTTGTACAGCTAAAGTG	5	0.125	No Hit
ACTCTAGGAAAAAGTAAAACAAAAACAAAACCTTCAGTGTACATGTCAGC	5	0.125	No Hit
TCTTCAGTGTACATGTCAGCTGCTTTTGAAGCATCATAAGTACCTGGTTT	5	0.125	No Hit
TCTTATATTGTCTCCAGCAAAAGGTGTAGCCCAGATTAAAGGTGTGTTCC	5	0.125	No Hit
AGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	5	0.125	No Hit
TCCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGG	5	0.125	No Hit
AGCCAGGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACAC	5	0.125	No Hit
TCTCTAAACTGTCTGGGGTCTCCAGTCTCTTGACAGTTAGGTGCATCTTC	5	0.125	No Hit
AGCTAGTCATACCAAACCCACCCAAAACTAGACCAAATAGTCAACTTCAG	5	0.125	No Hit
AGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	5	0.125	No Hit
TATCAGTCAACCCAGAATCAGAGATAATAGAAGAAACAGCTCTTTTTCTG	5	0.125	No Hit
ATGTGAGTTGGGTTGCTTGTGTTGTTTTAATTTTTTTTGAAAATTGTTTT	5	0.125	No Hit
AGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAA	5	0.125	No Hit
AGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCATAA	5	0.125	No Hit
AGCCATTATGTGGAGGAGCTGTTCAGCTCTCTCTAACCTATTTAGATTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0375	0.0
74-75	0.0	0.0	0.0	0.1875	0.0
76-77	0.0	0.0	0.0	0.225	0.0
78-79	0.0	0.0	0.0	0.3375	0.0
80-81	0.0	0.0	0.0	0.425	0.0
82-83	0.0	0.0	0.0	0.55	0.0
84-85	0.0	0.0	0.0	0.6875	0.0
86-87	0.0	0.0	0.0	0.775	0.0
88-89	0.0	0.0	0.0	0.8875	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCACCT	20	0.0019257745	71.25	8
CCACCTC	20	0.0019257745	71.25	9
CCACCAC	20	0.0019257745	71.25	6
CACCACC	20	0.0019257745	71.25	7
AAACCGA	15	0.009957196	47.5	90-91
AACCGAT	15	0.009957196	47.5	90-91
GATGTTT	15	0.009957196	47.5	94-95
GTCTGGT	30	0.009594663	47.5	2
ACCGATG	15	0.009957196	47.5	92-93
CTGAATC	15	0.009957196	47.5	82-83
TCAAAAC	15	0.009957196	47.5	86-87
GAATCAA	15	0.009957196	47.5	84-85
AAAACCG	15	0.009957196	47.5	88-89
CGATGTT	15	0.009957196	47.5	94-95
TGAATCA	15	0.009957196	47.5	82-83
CCGATGT	15	0.009957196	47.5	92-93
TTTTCTG	25	0.001521768	38.0	68-69
AAACCCA	30	0.0037229648	31.666668	30-31
>>END_MODULE
SRR32569737 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569737_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	18.973	12.0	12.0	26.0	12.0	34.0
2	31.888	34.0	34.0	34.0	26.0	34.0
3	32.4265	34.0	34.0	34.0	26.0	34.0
4	31.5365	34.0	34.0	34.0	26.0	34.0
5	32.3135	34.0	34.0	34.0	26.0	34.0
6	32.2215	34.0	34.0	34.0	26.0	34.0
7	31.908	34.0	34.0	34.0	26.0	34.0
8	32.2295	34.0	34.0	34.0	26.0	34.0
9	32.449	34.0	34.0	34.0	26.0	34.0
10-11	32.312	34.0	34.0	34.0	26.0	34.0
12-13	32.3185	34.0	34.0	34.0	26.0	34.0
14-15	32.37625	34.0	34.0	34.0	26.0	34.0
16-17	32.35725	34.0	34.0	34.0	26.0	34.0
18-19	32.2945	34.0	34.0	34.0	26.0	34.0
20-21	32.31	34.0	34.0	34.0	26.0	34.0
22-23	32.311499999999995	34.0	34.0	34.0	26.0	34.0
24-25	32.15375	34.0	34.0	34.0	26.0	34.0
26-27	32.3505	34.0	34.0	34.0	26.0	34.0
28-29	32.5065	34.0	34.0	34.0	26.0	34.0
30-31	32.411249999999995	34.0	34.0	34.0	26.0	34.0
32-33	32.379000000000005	34.0	34.0	34.0	26.0	34.0
34-35	32.03375	34.0	34.0	34.0	26.0	34.0
36-37	32.437749999999994	34.0	34.0	34.0	26.0	34.0
38-39	32.39375	34.0	34.0	34.0	26.0	34.0
40-41	32.4155	34.0	34.0	34.0	26.0	34.0
42-43	32.199	34.0	34.0	34.0	26.0	34.0
44-45	32.212	34.0	34.0	34.0	26.0	34.0
46-47	32.24125	34.0	34.0	34.0	26.0	34.0
48-49	32.20425	34.0	34.0	34.0	26.0	34.0
50-51	32.08475	34.0	34.0	34.0	26.0	34.0
52-53	32.043	34.0	34.0	34.0	26.0	34.0
54-55	32.026	34.0	34.0	34.0	26.0	34.0
56-57	31.93775	34.0	34.0	34.0	26.0	34.0
58-59	32.056250000000006	34.0	34.0	34.0	26.0	34.0
60-61	31.984	34.0	34.0	34.0	26.0	34.0
62-63	31.9895	34.0	34.0	34.0	26.0	34.0
64-65	31.88175	34.0	34.0	34.0	26.0	34.0
66-67	31.838500000000003	34.0	34.0	34.0	26.0	34.0
68-69	31.79875	34.0	34.0	34.0	26.0	34.0
70-71	31.756999999999998	34.0	34.0	34.0	26.0	34.0
72-73	31.739	34.0	34.0	34.0	26.0	34.0
74-75	31.65025	34.0	34.0	34.0	26.0	34.0
76-77	31.62225	34.0	34.0	34.0	26.0	34.0
78-79	31.61525	34.0	34.0	34.0	26.0	34.0
80-81	31.59525	34.0	34.0	34.0	26.0	34.0
82-83	31.5135	34.0	34.0	34.0	26.0	34.0
84-85	31.50975	34.0	34.0	34.0	26.0	34.0
86-87	31.41975	34.0	34.0	34.0	26.0	34.0
88-89	31.511	34.0	34.0	34.0	26.0	34.0
90-91	31.36875	34.0	34.0	34.0	26.0	34.0
92-93	31.335250000000002	34.0	34.0	34.0	26.0	34.0
94-95	31.189500000000002	34.0	34.0	34.0	26.0	34.0
96-97	30.67175	34.0	34.0	34.0	12.0	34.0
98-99	31.080750000000002	34.0	34.0	34.0	19.0	34.0
100-101	30.842	34.0	34.0	34.0	12.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	11.0
24	18.0
25	37.0
26	56.0
27	92.0
28	131.0
29	186.0
30	348.0
31	679.0
32	1430.0
33	1009.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.599999999999998	32.6	27.950000000000003	15.85
2	29.302558956347212	26.743602609131962	19.066733567486203	24.88710486703462
3	21.375	35.425000000000004	31.55	11.65
4	16.225	34.125	33.775	15.875
5	17.625	40.675	24.525	17.175
6	19.025	36.449999999999996	27.175	17.349999999999998
7	16.825000000000003	41.099999999999994	24.075	18.0
8	17.075000000000003	20.825	43.375	18.725
9	17.125	31.35	30.349999999999998	21.175
10-11	18.2375	34.25	30.15	17.3625
12-13	20.674999999999997	30.049999999999997	26.637499999999996	22.6375
14-15	17.0375	31.662499999999998	31.974999999999998	19.325
16-17	18.224999999999998	31.8	29.275000000000002	20.7
18-19	17.7	32.824999999999996	30.55	18.925
20-21	19.3875	32.1375	30.112499999999997	18.3625
22-23	17.599999999999998	32.7125	28.875	20.8125
24-25	17.4375	33.725	29.0875	19.75
26-27	18.075	33.275	28.599999999999998	20.05
28-29	18.375	31.075000000000003	30.875000000000004	19.675
30-31	18.1875	31.8	30.275000000000002	19.7375
32-33	18.7	31.2625	29.3375	20.7
34-35	18.124288064801924	31.325148715352487	31.61625110745475	18.934312112390835
36-37	18.462500000000002	32.475	30.7125	18.35
38-39	18.512500000000003	29.2375	32.275	19.975
40-41	19.675	33.2	28.787499999999998	18.337500000000002
42-43	16.8625	32.4375	31.0625	19.6375
44-45	17.2375	32.800000000000004	30.7625	19.2
46-47	18.325	30.575000000000003	30.912499999999998	20.1875
48-49	16.075	33.2	31.15	19.575
50-51	17.675	32.4125	31.2875	18.625
52-53	16.6125	33.35	29.725	20.3125
54-55	17.599999999999998	31.912499999999998	30.725	19.7625
56-57	18.0375	32.2625	29.125	20.575
58-59	18.2	30.675	30.925000000000004	20.200000000000003
60-61	17.9	32.5	31.5	18.099999999999998
62-63	19.125	30.475	30.0875	20.3125
64-65	17.675	31.775	31.5375	19.0125
66-67	19.412499999999998	30.7	30.1375	19.75
68-69	17.4375	31.025000000000002	32.6125	18.925
70-71	18.625	29.8375	33.35	18.1875
72-73	18.1875	31.4375	31.4625	18.912499999999998
74-75	18.7375	31.7625	30.65	18.85
76-77	18.0125	31.424999999999997	31.2125	19.35
78-79	18.101075806855143	28.8591443582687	32.261696272204155	20.778083562672002
80-81	18.75	30.312499999999996	32.95	17.9875
82-83	18.375	31.5625	31.7625	18.3
84-85	18.0	31.5125	31.887500000000003	18.6
86-87	17.1	31.5375	32.0	19.3625
88-89	16.2125	30.8125	32.05	20.925
90-91	18.5	30.612499999999997	31.874999999999996	19.0125
92-93	19.275000000000002	30.362499999999997	31.8	18.5625
94-95	19.4875	30.925000000000004	30.725	18.862499999999997
96-97	18.775201612903224	31.060987903225808	30.58215725806452	19.581653225806452
98-99	16.6875	30.625000000000004	31.637500000000003	21.05
100-101	17.4125	31.8125	30.875000000000004	19.900000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	0.5
16	1.5
17	3.5
18	4.0
19	4.5
20	6.5
21	8.5
22	9.0
23	14.0
24	26.0
25	37.5
26	52.5
27	62.0
28	85.0
29	98.0
30	129.5
31	177.0
32	199.5
33	205.5
34	191.0
35	183.5
36	183.5
37	180.5
38	180.0
39	207.5
40	199.0
41	181.5
42	224.0
43	245.5
44	217.5
45	161.0
46	98.5
47	75.5
48	68.0
49	76.5
50	68.5
51	44.0
52	37.5
53	28.0
54	14.5
55	3.0
56	2.5
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	1.5
65	1.5
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.35000000000000003
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.2375
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.075
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.8
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50173010380622	54.55
2	16.055363321799305	23.200000000000003
3	4.982698961937716	10.8
4	2.110726643598616	6.1
5	0.8650519031141869	3.125
6	0.34602076124567477	1.5
7	0.10380622837370243	0.525
8	0.03460207612456747	0.2
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGACTAGCAGGAGGTGGTGGAGAGAACATTCTATAGAGAGATTCTATCAC	8	0.2	No Hit
GTGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAAC	7	0.17500000000000002	No Hit
CCACAGACTGTGGCCACAAACCAGGTACTTATGATGCTTCAAAAGCAGCT	7	0.17500000000000002	No Hit
AAGGGCGGATGCCACCATTGTCTGAAAAGCAGCAACTATGCCAGTGAAAA	7	0.17500000000000002	No Hit
CGGAATCTGTACACACCTGGGTCCCCATCCCTACTCTATCTCTATACCTC	6	0.15	No Hit
AGGAATGTGAAAGCTGGTAAAGCACTTGGGTTAGAGACCGCCAGGCCTCT	6	0.15	No Hit
AGGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGT	6	0.15	No Hit
ACAACTTGTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGT	6	0.15	No Hit
TTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCT	6	0.15	No Hit
CTGAAAATAAAAGTGTATTAGAAAAACCTAGCTTTTAGCATATGTGATTA	6	0.15	No Hit
GTTTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAGATGAAGA	6	0.15	No Hit
AGTTATTTTGTGCAAAACAATTTTCAAAAAAAATTAAAACAACACAAGCA	6	0.15	No Hit
GCTTGCTTCTACTTAAAAAAATAAAAATAAAATAAAAATAAAAAAACAAC	6	0.15	No Hit
GCTGATATATTACCTTCACAAAACCGTTTTCATGAACCTGATGTCCAACA	6	0.15	No Hit
CGGTGAGGCCAACTGATCCTCTGCTGGACAATAAGTAACAGGTATTATTA	5	0.125	No Hit
TGGGATTTCTAAACACAGTGCAAACACTCCCAAGTCCCTATGTAGTATTG	5	0.125	No Hit
AGGAATATATTCCATCCAGGTATAGTGACAAGAGGGTATGTGGTTCAATG	5	0.125	No Hit
GGGTGTGTGCAGGCAACGTGAAGATATATCACCCGGACATCACGGTGCCT	5	0.125	No Hit
CTGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAAC	5	0.125	No Hit
TCTTCTCTATCTATAGATGAGAAGCCTGGAAAGCAGACTTTCTTTATATT	5	0.125	No Hit
GGTGATTATTTGTAAATACAACTGCCAATTTTATGGTTACAGCCAACTAA	5	0.125	No Hit
AGAAAGACACAGACACAAAGAGAAAAATCTTCTACTTCCTAAAAGAACTA	5	0.125	No Hit
GATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCT	5	0.125	No Hit
AGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCAC	5	0.125	No Hit
ACTACAGTGCTAGAATAGAGTTTGTTAGGATAGTCAGGTCATGTAAGAAC	5	0.125	No Hit
CAAAAGAGTGAGGATATTTCTTCACCTACCTCACAGTGTGTTATAAGGAA	5	0.125	No Hit
TTGAAAATAAAAGTGTATTAGAAAAACCTAGCTTTTAGCATATGTGATTA	5	0.125	No Hit
CTTTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAGATGAAGA	5	0.125	No Hit
GCTATTTTGGACACTACCCAGAACACAGACTGTGGCCACAAACCAGGTAC	5	0.125	No Hit
TCTTTTCATAGGCAAATGAAAGACACAGACACAAAGAGAAAAATCTTCTA	5	0.125	No Hit
AATCCTTTTTATCATAAAAATAAACATCGGTTTTGATTCAGTTTTATACA	5	0.125	No Hit
TGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCA	5	0.125	No Hit
CTCTGGATTTAAAGCTAAGACAAATATGCTGTTAAAAATGAGAGAATAAT	5	0.125	No Hit
GGTGGAGAATAGCATAGTTAGCCTTAATGAGAATCTTGCTTCTACTTAAA	5	0.125	No Hit
TGAAAAATCTTCTACTTCCTAAAAGAACTATTTCCCAGCTTATTTATGTC	5	0.125	No Hit
GAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAAATCTAA	5	0.125	No Hit
ACACGGTGCCTGGCCTCGGATGCAAACCTGCCAGTCCTGTCTCTTACTTT	5	0.125	No Hit
GGTTTTTCTGGGTATGAAACCATCATACTTGGTGAGCCTGCTTCCAGATG	5	0.125	No Hit
TAATTAATCCCTCATATGGACACCAAGTGTTTCCATAGATGGACCATTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0375	0.0
74-75	0.0	0.0	0.0	0.1875	0.0
76-77	0.0	0.0	0.0	0.225	0.0
78-79	0.0	0.0	0.0	0.3375	0.0
80-81	0.0	0.0	0.0	0.425	0.0
82-83	0.0	0.0	0.0	0.55	0.0
84-85	0.0	0.0	0.0	0.6875	0.0
86-87	0.0	0.0	0.0	0.725	0.0
88-89	0.0	0.0	0.0	0.825	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043847 spots for SRR32569737.sra
Written 11043847 spots for SRR32569737.sra
Read 11043850 spots for SRR32569737.sra
Written 11043850 spots for SRR32569737.sra
SRR ids: ['SRR32569737.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_z9lj4e2p
SRR32569737.sra spots: 88350779
blocks: [[1, 11043847], [11043848, 22087694], [22087695, 33131541], [33131542, 44175388], [44175389, 55219235], [55219236, 66263082], [66263083, 77306929], [77306930, 88350779]]
SRR32569737 file size 21375780
SRR32569737 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32569737 SRR32569737_1.fastq SRR32569737_2.fastq
Input file:	SRR32569737_1.fastq
Paired file:	SRR32569737_2.fastq
trimmed:	SRR32569737-trimmed-pair1.fastq, SRR32569737-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 05:52:27 2026 >> started

Tue Mar 31 05:56:16 2026 >> done (229.520s)
88350779 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
88350779 (100.00%) read pairs available; of these:
    7848 ( 0.01%) trimmed read pairs available after processing
88342931 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	       2	  0.00%
 95	       0	  0.00%
 96	      12	  0.00%
 97	       2	  0.00%
 98	      73	  0.00%
 99	     383	  0.00%
100	    7376	  0.01%
101	88342931	 99.99%
88350779 reads passed initial QC


criterion=sequence-density
sequence-density=10.03
sequence-density-rank=1
fanout-score=2.12
fanout-score-rank=9
prefix-density=10.87
prefix-fanout=2.0
sequence=TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGAT


criterion=fanout-score
sequence-density=0.22
sequence-density-rank=28
fanout-score=55.89
fanout-score-rank=1
prefix-density=6.27
prefix-fanout=1.9
sequence=AGTGAAATTCCT


criterion=sequence-density
sequence-density=1.89
sequence-density-rank=1
fanout-score=4.06
fanout-score-rank=20
prefix-density=7.45
prefix-fanout=1.0
sequence=TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=534.69
fanout-score-rank=1
prefix-density=5.20
prefix-fanout=1.0
sequence=GGCCTTTTACCTCATACATTTAC
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGAT -y TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAG -o SRR32569737 SRR32569737_1.fastq SRR32569737_2.fastq
Input file:	SRR32569737_1.fastq
Paired file:	SRR32569737_2.fastq
trimmed:	SRR32569737-trimmed-pair1.fastq, SRR32569737-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTG
-- paired 3' end adapter sequence (-y):	TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 06:04:22 2026 >> started

Tue Mar 31 06:06:32 2026 >> done (130.144s)
63107699 read pairs processed; of these:
     176 ( 0.00%) short read pairs filtered out after trimming by size control
     786 ( 0.00%) empty read pairs filtered out after trimming by size control
63106737 (100.00%) read pairs available; of these:
     379 ( 0.00%) trimmed read pairs available after processing
63106358 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	      10	  0.00%
 95	       1	  0.00%
 96	      11	  0.00%
 97	      11	  0.00%
 98	      84	  0.00%
 99	     391	  0.00%
100	    5442	  0.01%
101	63100787	 99.99%


criterion=sequence-density
sequence-density=1.81
sequence-density-rank=1
fanout-score=58.99
fanout-score-rank=3
prefix-density=2.29
prefix-fanout=46.7
sequence=ACTGTCTCTTATACACATCTCCGAGCCCACGAGACATGGTTGACTATCTCGTATGCCGTCTTCTGCTTGAAAA


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=14
fanout-score=116.95
fanout-score-rank=1
prefix-density=0.47
prefix-fanout=16.2
sequence=GGGGTGGGGTGGGG


criterion=sequence-density
sequence-density=1.85
sequence-density-rank=1
fanout-score=41.36
fanout-score-rank=2
prefix-density=2.39
prefix-fanout=32.1
sequence=ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGACAGGCCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=6
fanout-score=351.18
fanout-score-rank=1
prefix-density=1.14
prefix-fanout=31.7
sequence=CACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAG
SRR32569737 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 06:11:29
                             Started mapping on |	Mar 31 06:11:29
                                    Finished on |	Mar 31 06:22:18
       Mapping speed, Million of reads per hour |	490.08

                          Number of input reads |	88349817
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	80547878
                        Uniquely mapped reads % |	91.17%
                          Average mapped length |	198.39
                       Number of splices: Total |	45359332
            Number of splices: Annotated (sjdb) |	44912461
                       Number of splices: GT/AG |	44880222
                       Number of splices: GC/AG |	368190
                       Number of splices: AT/AC |	50535
               Number of splices: Non-canonical |	60385
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.80
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.27
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4479634
             % of reads mapped to multiple loci |	5.07%
        Number of reads mapped to too many loci |	156314
             % of reads mapped to too many loci |	0.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.57%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3322305	3322305	3322305
N_multimapping	4479634	4479634	4479634
N_noFeature	5312636	77438089	5875643
N_ambiguous	3541166	179479	1026395
UnstrandedReadsAssigned:71694076 PositiveStrandReadsAssigned:2930310 NegativeStrandReadsAssigned:73645840
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32569737 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32569737-trimmed-pair1.fastq
                             SRR32569737-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 88,349,817 reads, 78,135,936 reads pseudoaligned
[quant] estimated average fragment length: 190.636
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,263 rounds

 109283 SRR32569737.ke.tsv
  52636 SRR32569737.se.tsv
 161919 total
==> SRR32569737.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9	0	0
ENSMUST00000196221.1	9	3.75	0	0
ENSMUST00000179664.1	11	5.75	0	0
ENSMUST00000178537.1	12	6.75	0	0
ENSMUST00000178862.1	14	8.75	0	0
ENSMUST00000179520.1	11	5.75	0	0
ENSMUST00000179883.1	16	9	0	0
ENSMUST00000195858.1	10	4.75	0	0
ENSMUST00000179932.1	12	6.75	0	0

==> SRR32569737.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2635
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	4
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32569737 completed mapping pipeline successfully
