Starting /dee2/code/volunteer_pipeline.sh SRR32569730
    current disk space = 828222004
    free memory = 196940564 
SRR32569730 SRAfilesize
df0e0b7612e3800493953079e141d460  SRR32569730.sra
SRR32569730.sra file validated
SRR32569730 is paired end
SRR32569730 is conventional basespace
SRR32569730 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.723	26.0	12.0	34.0	12.0	34.0
2	32.8355	34.0	34.0	34.0	34.0	34.0
3	33.2605	34.0	34.0	34.0	34.0	34.0
4	33.069	34.0	34.0	34.0	34.0	34.0
5	33.2455	34.0	34.0	34.0	34.0	34.0
6	33.134	34.0	34.0	34.0	34.0	34.0
7	33.1615	34.0	34.0	34.0	34.0	34.0
8	33.092	34.0	34.0	34.0	34.0	34.0
9	33.0655	34.0	34.0	34.0	34.0	34.0
10-11	33.10325	34.0	34.0	34.0	34.0	34.0
12-13	33.07625	34.0	34.0	34.0	34.0	34.0
14-15	33.035	34.0	34.0	34.0	34.0	34.0
16-17	33.13225	34.0	34.0	34.0	34.0	34.0
18-19	33.12375	34.0	34.0	34.0	34.0	34.0
20-21	33.02475	34.0	34.0	34.0	34.0	34.0
22-23	32.94075	34.0	34.0	34.0	34.0	34.0
24-25	32.93575	34.0	34.0	34.0	34.0	34.0
26-27	33.032250000000005	34.0	34.0	34.0	34.0	34.0
28-29	33.0385	34.0	34.0	34.0	34.0	34.0
30-31	33.006	34.0	34.0	34.0	34.0	34.0
32-33	33.01925	34.0	34.0	34.0	34.0	34.0
34-35	33.0415	34.0	34.0	34.0	34.0	34.0
36-37	33.05475	34.0	34.0	34.0	34.0	34.0
38-39	32.839749999999995	34.0	34.0	34.0	34.0	34.0
40-41	32.903	34.0	34.0	34.0	34.0	34.0
42-43	32.9865	34.0	34.0	34.0	34.0	34.0
44-45	32.876000000000005	34.0	34.0	34.0	34.0	34.0
46-47	32.74425	34.0	34.0	34.0	34.0	34.0
48-49	32.70375	34.0	34.0	34.0	34.0	34.0
50-51	32.65925	34.0	34.0	34.0	30.0	34.0
52-53	32.66175	34.0	34.0	34.0	30.0	34.0
54-55	32.564	34.0	34.0	34.0	30.0	34.0
56-57	32.5295	34.0	34.0	34.0	30.0	34.0
58-59	32.46325	34.0	34.0	34.0	26.0	34.0
60-61	32.525000000000006	34.0	34.0	34.0	30.0	34.0
62-63	32.54525	34.0	34.0	34.0	34.0	34.0
64-65	32.4755	34.0	34.0	34.0	26.0	34.0
66-67	32.49075	34.0	34.0	34.0	30.0	34.0
68-69	32.5185	34.0	34.0	34.0	26.0	34.0
70-71	32.423500000000004	34.0	34.0	34.0	26.0	34.0
72-73	32.4025	34.0	34.0	34.0	26.0	34.0
74-75	32.49425	34.0	34.0	34.0	26.0	34.0
76-77	32.25375	34.0	34.0	34.0	26.0	34.0
78-79	32.308	34.0	34.0	34.0	26.0	34.0
80-81	32.3725	34.0	34.0	34.0	26.0	34.0
82-83	32.2615	34.0	34.0	34.0	26.0	34.0
84-85	32.2445	34.0	34.0	34.0	26.0	34.0
86-87	32.211	34.0	34.0	34.0	26.0	34.0
88-89	32.241749999999996	34.0	34.0	34.0	26.0	34.0
90-91	32.14	34.0	34.0	34.0	26.0	34.0
92-93	31.98275	34.0	34.0	34.0	26.0	34.0
94-95	31.981749999999998	34.0	34.0	34.0	26.0	34.0
96-97	32.0	34.0	34.0	34.0	26.0	34.0
98-99	31.88825	34.0	34.0	34.0	26.0	34.0
100-101	31.911	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	4.0
25	8.0
26	40.0
27	44.0
28	61.0
29	102.0
30	204.0
31	385.0
32	1066.0
33	2048.0
34	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.75	54.900000000000006	43.675000000000004	0.675
2	38.35	11.875	12.174999999999999	37.6
3	24.7	12.85	37.8	24.65
4	24.55	18.475	25.55	31.424999999999997
5	21.275	29.25	25.05	24.425
6	23.425	31.1	27.925	17.549999999999997
7	22.95	34.75	24.925	17.375
8	15.35	27.05	44.3	13.3
9	18.8	26.650000000000002	38.925	15.625
10-11	16.662499999999998	32.2125	37.4625	13.6625
12-13	21.8875	26.900000000000002	32.2125	19.0
14-15	16.9375	29.725	36.1125	17.224999999999998
16-17	18.25	28.6875	35.75	17.3125
18-19	19.925	28.225	34.7375	17.1125
20-21	20.4875	31.05	31.900000000000002	16.5625
22-23	21.8625	31.937500000000004	30.9625	15.2375
24-25	21.15	30.012499999999996	30.587500000000002	18.25
26-27	21.224999999999998	28.799999999999997	31.0375	18.9375
28-29	18.787499999999998	28.762500000000003	31.775	20.674999999999997
30-31	19.662499999999998	29.5875	32.550000000000004	18.2
32-33	16.7375	30.2	33.5875	19.475
34-35	16.425	31.662499999999998	33.4125	18.5
36-37	17.4875	31.775	33.287499999999994	17.45
38-39	15.675	31.45	33.175	19.7
40-41	18.35	28.475	35.35	17.825
42-43	19.875	29.625	32.5125	17.9875
44-45	19.3125	32.725	30.662499999999998	17.299999999999997
46-47	18.987499999999997	31.337500000000002	31.55	18.125
48-49	19.2625	33.15	30.862499999999997	16.725
50-51	18.9375	33.175	30.675	17.2125
52-53	19.4875	28.349999999999998	32.225	19.9375
54-55	19.112499999999997	33.575	29.9	17.4125
56-57	18.125	34.325	29.4	18.15
58-59	15.725	32.2125	32.45	19.6125
60-61	18.375	33.425	29.599999999999998	18.6
62-63	16.325	32.9625	32.35	18.3625
64-65	18.6125	31.624999999999996	31.137500000000003	18.625
66-67	18.099999999999998	31.3125	34.7875	15.8
68-69	19.45	30.575000000000003	32.425	17.549999999999997
70-71	15.6375	32.2125	32.1875	19.9625
72-73	18.125	30.3	31.05	20.525
74-75	17.875	32.7625	31.8125	17.549999999999997
76-77	17.025000000000002	32.487500000000004	33.6125	16.875
78-79	16.7875	31.9875	33.862500000000004	17.3625
80-81	17.95	31.4625	34.375	16.2125
82-83	18.7625	31.6875	32.7125	16.8375
84-85	19.5875	28.762500000000003	33.85	17.8
86-87	18.712500000000002	29.975	34.125	17.1875
88-89	19.0625	28.749999999999996	34.75	17.4375
90-91	20.4	33.15	29.225	17.224999999999998
92-93	17.9875	33.4875	31.587500000000002	16.9375
94-95	17.349999999999998	31.825	32.4625	18.3625
96-97	18.75	32.85	31.7625	16.6375
98-99	19.75	31.137500000000003	30.7	18.4125
100-101	17.424999999999997	32.65	31.775	18.15
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	2.0
17	11.5
18	16.0
19	13.5
20	19.5
21	26.0
22	28.0
23	18.5
24	10.0
25	22.5
26	38.5
27	58.0
28	84.0
29	106.5
30	177.5
31	257.5
32	255.5
33	220.5
34	209.0
35	168.0
36	156.0
37	154.5
38	152.5
39	177.0
40	163.0
41	203.0
42	231.0
43	184.5
44	155.5
45	144.0
46	104.5
47	71.5
48	77.5
49	83.0
50	70.5
51	55.0
52	37.0
53	22.0
54	10.5
55	0.5
56	1.0
57	1.5
58	0.5
59	0.0
60	0.0
61	0.5
62	0.5
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.20094191522763	26.85
2	19.570905285191	18.7
3	10.622710622710622	15.225
4	4.552590266875981	8.7
5	3.610675039246468	8.625
6	1.465201465201465	4.2
7	1.3605442176870748	4.55
8	0.9419152276295133	3.5999999999999996
9	0.4186289900575615	1.7999999999999998
>10	1.2558869701726845	7.75
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	23	0.575	No Hit
AGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	20	0.5	No Hit
ACTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTTT	16	0.4	No Hit
TGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	15	0.375	No Hit
AGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	15	0.375	No Hit
TCCCACTTACTGTTCTTACATGACCTGACTATCCTAACAAACTCTATTCT	13	0.325	No Hit
ACTGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAA	13	0.325	No Hit
TCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	13	0.325	No Hit
ACTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTG	13	0.325	No Hit
AGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	13	0.325	No Hit
ACCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	12	0.3	No Hit
AGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	12	0.3	No Hit
ACTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	12	0.3	No Hit
TGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	12	0.3	No Hit
TGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	12	0.3	No Hit
TCCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	12	0.3	No Hit
TCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	11	0.27499999999999997	No Hit
TCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTG	11	0.27499999999999997	No Hit
TGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	11	0.27499999999999997	No Hit
TCTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCA	11	0.27499999999999997	No Hit
AGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTG	10	0.25	No Hit
TGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	10	0.25	No Hit
AGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	10	0.25	No Hit
ACCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	10	0.25	No Hit
AGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	9	0.22499999999999998	No Hit
ACCCTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGG	9	0.22499999999999998	No Hit
AGGGGGAAAAAATGAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAG	9	0.22499999999999998	No Hit
TGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	9	0.22499999999999998	No Hit
AGGGCAGCAGCCTCCAACCAAGAAAGACACACTTACAAGTTGAATTCTAA	9	0.22499999999999998	No Hit
ACTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATACA	9	0.22499999999999998	No Hit
AGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCCATCTGGCTACAGAGA	9	0.22499999999999998	No Hit
AGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	9	0.22499999999999998	No Hit
AGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTA	8	0.2	No Hit
TCTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTG	8	0.2	No Hit
TGTCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGG	8	0.2	No Hit
TCCCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGT	8	0.2	No Hit
ACGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGG	8	0.2	No Hit
ATGGCATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTT	8	0.2	No Hit
AGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCAT	8	0.2	No Hit
AGTCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGG	8	0.2	No Hit
AGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	8	0.2	No Hit
ACCCACTTACTGTTCTTACATGACCTGACTATCCTAACAAACTCTATTCT	8	0.2	No Hit
TGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTGTG	8	0.2	No Hit
TGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	8	0.2	No Hit
ACCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	8	0.2	No Hit
AGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAA	8	0.2	No Hit
TTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAAT	8	0.2	No Hit
ACCCCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATG	8	0.2	No Hit
TCTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGAAGATAGTCATC	8	0.2	No Hit
TGGAAAAAATGAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGA	8	0.2	No Hit
AGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	7	0.17500000000000002	No Hit
AGGCATGTTGTTGGTACCTGTACCATTCTCTTCCATTGAGAATTCTAAGC	7	0.17500000000000002	No Hit
AGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATT	7	0.17500000000000002	No Hit
TCTAGGTTTTTCTAATACACTTTTATTTTCATGGAGGGCTCTAGAGGATT	7	0.17500000000000002	No Hit
TCTCATCTTAATAATACCTGTTACTTATTGTCCAGCAGAGGATCAGTTGG	7	0.17500000000000002	No Hit
TCCTCTGCATTTATTCTTGAGGTTACTGAGCCATCTGGCTACAGAGAAGA	7	0.17500000000000002	No Hit
AATGCTATTCTCCACTGCGCCCCATTGATCAGACAATGGCTTTAAGTGGG	7	0.17500000000000002	No Hit
AGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTGTG	7	0.17500000000000002	No Hit
AGCTCTCTCTAACCTATTTAGATTTTATCTTTCACTTTGTGATAGAATCT	7	0.17500000000000002	No Hit
TCTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATACA	7	0.17500000000000002	No Hit
AGTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGA	7	0.17500000000000002	No Hit
TGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAA	7	0.17500000000000002	No Hit
AGGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATAT	7	0.17500000000000002	No Hit
ACACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	7	0.17500000000000002	No Hit
AAGCTGAGGTAGTTAAATGTGAAACTTACACAACTTAAAATAATGAGAGA	7	0.17500000000000002	No Hit
ACTCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGG	7	0.17500000000000002	No Hit
TCTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATA	7	0.17500000000000002	No Hit
AATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATATGA	7	0.17500000000000002	No Hit
AGCTGCTTTTGAAGCATCATAAGTACCTGGTTTGTGGCCACAGTCTGTGT	7	0.17500000000000002	No Hit
AGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	7	0.17500000000000002	No Hit
ACTACCATCTCTCCACATTTTAAAGCCTTTGTGGAGCTCACATCTGTGTA	7	0.17500000000000002	No Hit
TCTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTTT	7	0.17500000000000002	No Hit
TCTCCACTGCGCCCCATTGATCAGACAATGGCTTTAAGTGGGGCCTAAAT	7	0.17500000000000002	No Hit
TCTGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAA	7	0.17500000000000002	No Hit
ACCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	7	0.17500000000000002	No Hit
AGGTGTCAATACTGTAAGTATTCCAAGCACAACTTAATTTCTAATTCACT	7	0.17500000000000002	No Hit
TGGGGCCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTG	6	0.15	No Hit
TCCTGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCCATCTGGCTACAG	6	0.15	No Hit
AGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAAATTCAAATAAA	6	0.15	No Hit
ACCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTT	6	0.15	No Hit
TCACAGATTGATGATCTCTAGGAAAAAGTAAAACAAAAACAAAACCTTCA	6	0.15	No Hit
TGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATT	6	0.15	No Hit
ACTCATTTTTAACAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTT	6	0.15	No Hit
AGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTTAA	6	0.15	No Hit
TCTCTATATATATTTACATCAAAGAATTTAATAACTCATTTAAATTTTTG	6	0.15	No Hit
ACTATGACTCAAAAATTACAAGTGTTTATAACCCTACTTAGTTTAGTCAT	6	0.15	No Hit
TGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCAGCTTTCACATTCCTTA	6	0.15	No Hit
AGTTGCAAAGATGTTGTGTTAGAAATGGTATAAAAACCCAGTTAAACCAT	6	0.15	No Hit
AGTTTATTTTTATGATAAAAAGGATATACTTTTGTATTTTGGGGTAGAAA	6	0.15	No Hit
AGTTGTGTTAGAAATGGTATAAAAACCCAGTTAAACCATTCCAAATCTCA	6	0.15	No Hit
AGTCTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACC	6	0.15	No Hit
TGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCAT	6	0.15	No Hit
AGCCCACTTACTGTTCTTACATGACCTGACTATCCTAACAAACTCTATTC	6	0.15	No Hit
AGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTGGTTTG	6	0.15	No Hit
ACCTGCACACACCTTATTTCTGAAATGGAGAGAGAAACTCAAGAATGTCA	6	0.15	No Hit
TCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGCA	6	0.15	No Hit
TGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	6	0.15	No Hit
TGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCATAA	6	0.15	No Hit
TCTTCAGTGTACATGTCAGCTGCTTTTGAAGCATCATAAGTACCTGGTTT	6	0.15	No Hit
ACTCATCTTAATAATACCTGTTACTTATTGTCCAGCAGAGGATCAGTTGG	6	0.15	No Hit
ACTTCTGGTTTGTCTTCATCTACCAGCTATTACTCCTCTTTTGCTCAGAG	6	0.15	No Hit
TCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGACT	6	0.15	No Hit
ACTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTC	6	0.15	No Hit
TCACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGT	6	0.15	No Hit
AGCCTAGACTTTTAATGGAAAGTTTTCACTGGCATAGTTGCTGCTTTTCA	5	0.125	No Hit
AGGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTTA	5	0.125	No Hit
TCTTTGGTGTACAGGGAAAGGAGAGACTCAAGAATGAATTTCAACATCTG	5	0.125	No Hit
AATCACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCA	5	0.125	No Hit
ACCCAGTTAAACCATTCCAAATCTCATCTTAATAATACCTGTTACTTATT	5	0.125	No Hit
TCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	5	0.125	No Hit
ATGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATT	5	0.125	No Hit
TCTCATTTAAATTTTTGTTTCAAATAAAATTCCAAATACATTTGTACAAT	5	0.125	No Hit
TCACACTTACAAGTTGAATTCTAAAGGAAGGTCTGGTTATGTGGGGTTGG	5	0.125	No Hit
TGCCTTTGTGGAGCTCACATCTGTGTATCCTATTTATGCAAGTAGCCATA	5	0.125	No Hit
TGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCA	5	0.125	No Hit
ATGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTGT	5	0.125	No Hit
TCTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	5	0.125	No Hit
AGTCCATATGAGGGATTAATTGTTCTCATTATAAAGTTTATAATTTCTGC	5	0.125	No Hit
TGCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCAC	5	0.125	No Hit
TCCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	5	0.125	No Hit
ACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATA	5	0.125	No Hit
TAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGA	5	0.125	No Hit
TACCAAACCCACCCAAAACTAGACCAAATAGTCAACTTCAGTTTTTCTGT	5	0.125	No Hit
ACCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	5	0.125	No Hit
TGTTTATTTTTATGATAAAAAGGATATACTTTTGTATTTTGGGGTAGAAA	5	0.125	No Hit
TAATTTATTTTGTAAATGAATTTAGTTATTAAATAAGAGTACTGAATGGA	5	0.125	No Hit
TGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGT	5	0.125	No Hit
TGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	5	0.125	No Hit
TCTGAAACATTTAAAATACATTTATAATATGGAGATGAATCAGCTCAAGA	5	0.125	No Hit
ATGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTCTCTTC	5	0.125	No Hit
ACTTTGAACAAGCACTTCACTGAAACATTTAAAATACATTTATAATATGG	5	0.125	No Hit
AGTACAGATTCCTTGCTTCTTTGTTGCTATCATTGAACCACATACCCTCT	5	0.125	No Hit
AGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	5	0.125	No Hit
TCTGGTTTGTGGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAA	5	0.125	No Hit
AGAGGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTT	5	0.125	No Hit
AGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAA	5	0.125	No Hit
AGCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCAC	5	0.125	No Hit
TCTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	5	0.125	No Hit
AGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGGGGACTCTA	5	0.125	No Hit
ATGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATAT	5	0.125	No Hit
ATCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAAT	5	0.125	No Hit
AGTTGACATAAATAAGCTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTT	5	0.125	No Hit
ACTGTTCTCTTCTGGTTTGTCTTCATCTACCAGCTATTACTCCTCTTTTG	5	0.125	No Hit
ACTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	5	0.125	No Hit
ACTCAAGAATGTCACCGACTATGCATCAGAATACTATGTACAGTTGAAGT	5	0.125	No Hit
TCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAAA	5	0.125	No Hit
AATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAACA	5	0.125	No Hit
AGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTT	5	0.125	No Hit
AGTGTGAGTTGGGTTGCTTGTGTTGTTTTAATTTTTTTTGAAAATTGTTT	5	0.125	No Hit
ACAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAGCA	5	0.125	No Hit
ACTCTCCACCACCTCCTGCTAGTCATACCAAACCCACCCAAAACTAGACC	5	0.125	No Hit
AGTGGCATCCGCCCTTCTTCTTCCTGTGATGCACACGGTTCTCCATGTTC	5	0.125	No Hit
ATACCATCTCTCCACATTTTAAAGCCTTTGTGGAGCTCACATCTGTGTAT	5	0.125	No Hit
AGGTTGACATAAATAAGCTGGGAAATAGTTCTTTTAGGAAGTAGAAGATT	5	0.125	No Hit
ACTCTTTTTCTGGGTATGATTGCTGTTAGCCTTTCTGTTTTAATATTAGC	5	0.125	No Hit
TGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAA	5	0.125	No Hit
TACAGCTCTCTCTAACCTATTTAGATTTTATCTTTCACTTTGTGATAGAA	5	0.125	No Hit
AGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTGG	5	0.125	No Hit
ACCTGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGA	5	0.125	No Hit
TGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTA	5	0.125	No Hit
ACCCAAACTTATGGGACACAATGAAAGCATTTCTAAGAGGAAAAGTCATA	5	0.125	No Hit
TGTCTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACC	5	0.125	No Hit
ATCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGC	5	0.125	No Hit
AGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATATG	5	0.125	No Hit
TCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAAAGCCTTT	5	0.125	No Hit
AGCACAGATTGATGATCTCTAGGAAAAAGTAAAACAAAAACAAAACCTTC	5	0.125	No Hit
ACGTCTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAAC	5	0.125	No Hit
AACTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	5	0.125	No Hit
AGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAA	5	0.125	No Hit
TCCTGCACACACCTTATTTCTGAAATGGAGAGAGAAACTCAAGAATGTCA	5	0.125	No Hit
ACTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCA	5	0.125	No Hit
AGGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGG	5	0.125	No Hit
AGGCAGTTGTATTTACAAATAATCACATATATGATGCTTACAGAATGATG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0125	0.0
72-73	0.0	0.0	0.0	0.025	0.0
74-75	0.0	0.0	0.0	0.1	0.0
76-77	0.0	0.0	0.0	0.1	0.0
78-79	0.0	0.0	0.0	0.1	0.0
80-81	0.0	0.0	0.0	0.1	0.0
82-83	0.0	0.0	0.0	0.225	0.0
84-85	0.0	0.0	0.0	0.3125	0.0
86-87	0.0	0.0	0.0	0.4875	0.0
88-89	0.0	0.0	0.0	0.625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACAG	15	6.142176E-4	95.0	1
>>END_MODULE
SRR32569730 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569730_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	18.7795	12.0	12.0	26.0	12.0	34.0
2	31.83	34.0	34.0	34.0	26.0	34.0
3	32.1135	34.0	34.0	34.0	26.0	34.0
4	31.633	34.0	34.0	34.0	26.0	34.0
5	31.87	34.0	34.0	34.0	26.0	34.0
6	31.704	34.0	34.0	34.0	26.0	34.0
7	31.863	34.0	34.0	34.0	26.0	34.0
8	32.1055	34.0	34.0	34.0	26.0	34.0
9	32.2165	34.0	34.0	34.0	26.0	34.0
10-11	32.061	34.0	34.0	34.0	26.0	34.0
12-13	32.198499999999996	34.0	34.0	34.0	26.0	34.0
14-15	32.2005	34.0	34.0	34.0	26.0	34.0
16-17	32.153499999999994	34.0	34.0	34.0	26.0	34.0
18-19	32.1435	34.0	34.0	34.0	26.0	34.0
20-21	32.10925	34.0	34.0	34.0	26.0	34.0
22-23	32.1185	34.0	34.0	34.0	26.0	34.0
24-25	32.1345	34.0	34.0	34.0	26.0	34.0
26-27	32.390249999999995	34.0	34.0	34.0	26.0	34.0
28-29	32.48925	34.0	34.0	34.0	26.0	34.0
30-31	32.2755	34.0	34.0	34.0	26.0	34.0
32-33	32.349999999999994	34.0	34.0	34.0	26.0	34.0
34-35	31.94825	34.0	34.0	34.0	26.0	34.0
36-37	32.156499999999994	34.0	34.0	34.0	26.0	34.0
38-39	32.31100000000001	34.0	34.0	34.0	26.0	34.0
40-41	32.182	34.0	34.0	34.0	26.0	34.0
42-43	32.117	34.0	34.0	34.0	26.0	34.0
44-45	32.17425	34.0	34.0	34.0	26.0	34.0
46-47	32.089749999999995	34.0	34.0	34.0	26.0	34.0
48-49	32.00725	34.0	34.0	34.0	26.0	34.0
50-51	31.972250000000003	34.0	34.0	34.0	26.0	34.0
52-53	31.79475	34.0	34.0	34.0	26.0	34.0
54-55	31.842750000000002	34.0	34.0	34.0	26.0	34.0
56-57	31.829749999999997	34.0	34.0	34.0	26.0	34.0
58-59	31.86	34.0	34.0	34.0	26.0	34.0
60-61	31.886249999999997	34.0	34.0	34.0	26.0	34.0
62-63	31.91875	34.0	34.0	34.0	26.0	34.0
64-65	31.774	34.0	34.0	34.0	26.0	34.0
66-67	31.66975	34.0	34.0	34.0	26.0	34.0
68-69	31.715249999999997	34.0	34.0	34.0	26.0	34.0
70-71	31.6355	34.0	34.0	34.0	26.0	34.0
72-73	31.6225	34.0	34.0	34.0	26.0	34.0
74-75	31.53875	34.0	34.0	34.0	26.0	34.0
76-77	31.414	34.0	34.0	34.0	26.0	34.0
78-79	31.43875	34.0	34.0	34.0	26.0	34.0
80-81	31.50025	34.0	34.0	34.0	26.0	34.0
82-83	31.42	34.0	34.0	34.0	26.0	34.0
84-85	31.2685	34.0	34.0	34.0	26.0	34.0
86-87	31.32475	34.0	34.0	34.0	26.0	34.0
88-89	31.22175	34.0	34.0	34.0	19.0	34.0
90-91	31.049	34.0	34.0	34.0	12.0	34.0
92-93	30.9965	34.0	34.0	34.0	12.0	34.0
94-95	31.0835	34.0	34.0	34.0	12.0	34.0
96-97	30.600250000000003	34.0	34.0	34.0	12.0	34.0
98-99	30.853250000000003	34.0	34.0	34.0	12.0	34.0
100-101	30.643	34.0	34.0	34.0	12.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	6.0
24	11.0
25	36.0
26	70.0
27	81.0
28	152.0
29	259.0
30	408.0
31	733.0
32	1404.0
33	834.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.324999999999996	29.099999999999998	28.625	16.950000000000003
2	28.772282199347227	25.48330404217926	17.901079588250063	27.84333417022345
3	19.8	37.625	30.3	12.275
4	18.7	32.625	30.8	17.875
5	17.7	40.0	26.224999999999998	16.075
6	19.7	37.4	27.400000000000002	15.5
7	15.675	41.449999999999996	24.55	18.325
8	14.924999999999999	23.35	43.375	18.35
9	16.400000000000002	31.324999999999996	30.475	21.8
10-11	17.0125	35.4375	29.7375	17.8125
12-13	20.3625	31.087500000000002	26.974999999999998	21.575
14-15	16.6875	32.1625	31.087500000000002	20.0625
16-17	18.0375	32.324999999999996	29.4875	20.150000000000002
18-19	17.8125	32.550000000000004	30.412499999999998	19.225
20-21	17.724999999999998	34.150000000000006	30.275000000000002	17.849999999999998
22-23	18.25	32.525	28.8875	20.3375
24-25	17.9	34.375	28.175	19.55
26-27	19.2	32.45	28.975	19.375
28-29	18.8	31.225	29.1125	20.8625
30-31	17.2375	33.650000000000006	30.5375	18.575
32-33	17.675	34.125	28.875	19.325
34-35	17.842690945877592	31.71471927162367	31.5756196256955	18.866970156803237
36-37	19.4375	31.2125	29.425	19.925
38-39	16.5125	31.412499999999998	32.0375	20.0375
40-41	19.5125	30.362499999999997	31.0125	19.112499999999997
42-43	16.875	30.9375	30.9375	21.25
44-45	17.8	32.1375	30.612499999999997	19.45
46-47	17.65	30.1875	31.1875	20.974999999999998
48-49	15.325	32.95	32.2625	19.4625
50-51	17.525	32.1625	30.9625	19.35
52-53	18.1375	31.587500000000002	30.599999999999998	19.675
54-55	17.2375	32.725	29.95	20.0875
56-57	16.925	33.575	30.3875	19.112499999999997
58-59	16.3375	33.050000000000004	31.412499999999998	19.2
60-61	18.025	31.1875	31.837500000000002	18.95
62-63	18.1375	31.912499999999998	30.2625	19.6875
64-65	17.9375	31.662499999999998	30.612499999999997	19.787499999999998
66-67	18.35	32.4375	30.112499999999997	19.1
68-69	17.275	33.525	31.662499999999998	17.5375
70-71	17.974999999999998	31.4625	32.175	18.387500000000003
72-73	18.512500000000003	33.125	30.012499999999996	18.35
74-75	17.2375	31.775	32.125	18.862499999999997
76-77	16.8125	30.887500000000003	31.724999999999998	20.575
78-79	16.75228668086706	30.873324144844005	30.08394937977697	22.290439794511965
80-81	17.549999999999997	31.4	31.937500000000004	19.112499999999997
82-83	16.900000000000002	30.65	33.475	18.975
84-85	17.825	32.737500000000004	31.0125	18.425
86-87	16.675	34.0	30.475	18.85
88-89	17.5	30.112499999999997	32.6875	19.7
90-91	18.15	30.7	32.0625	19.0875
92-93	18.65	34.075	29.462500000000002	17.8125
94-95	17.5875	33.1625	30.062499999999996	19.1875
96-97	17.513554406758292	32.278401210440045	31.206657420249652	19.00138696255201
98-99	17.175	31.137500000000003	33.725	17.962500000000002
100-101	17.65	30.2625	32.5625	19.525000000000002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	2.5
18	2.5
19	3.0
20	5.0
21	9.0
22	10.5
23	12.5
24	21.0
25	38.0
26	64.5
27	76.0
28	92.5
29	120.0
30	162.0
31	192.0
32	199.0
33	220.5
34	223.5
35	206.5
36	192.0
37	183.5
38	164.5
39	168.0
40	176.0
41	187.0
42	217.5
43	214.5
44	192.0
45	144.0
46	87.5
47	67.5
48	72.0
49	71.5
50	60.5
51	50.5
52	42.5
53	27.5
54	10.0
55	3.5
56	2.5
57	2.0
58	2.0
59	0.5
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.42500000000000004
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.15
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.2375
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.8625
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21052631578947	52.87500000000001
2	16.42105263157895	23.400000000000002
3	5.684210526315789	12.15
4	2.631578947368421	7.5
5	0.7017543859649122	2.5
6	0.2807017543859649	1.2
7	0.03508771929824561	0.17500000000000002
8	0.03508771929824561	0.2
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTAGCATAGAAAATTATGTGACTCCTCAAAAATATCTACAGTGCTAG	8	0.2	No Hit
TAAATTCTTTGATGTAAATATATATAGAGATATATAGATATACATATTCC	7	0.17500000000000002	No Hit
TAGAGACTGTACATATTTGCTTAATTTGCCTTAAATATTTTGTGCTTTTC	6	0.15	No Hit
AGAGAAAAATCTTCTACTTCCTAAAAGAACTATTTCCCAGCTTATTTATG	6	0.15	No Hit
ACCTGCTTTCCGGAGTGCTACAGCATGAAATGGGTGGGATCAGCTCTGCC	6	0.15	No Hit
TCAAAGCCTCATCACTAAGCTGTGTTTGTCTACCTGGTACGTTAACTATT	6	0.15	No Hit
TGAACAATTAATCCCTCATATGGACACCAAGTGTTTCCATAGATGGACCA	6	0.15	No Hit
TCAAAGAAGCAAGGAATCTGTACACACCTGGGTCCCCATCCCTACTCTAT	6	0.15	No Hit
AGACTTTCTGAAGCATGACTGTAGCATAGAAAATTATGTGACTCCTCAAA	6	0.15	No Hit
AGAGACCGCCAGGCCTCTCACTAAATTGCAGGAACATGGAGAACCGTGTG	6	0.15	No Hit
CCTTGTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTGAC	5	0.125	No Hit
TCATTATTTTAAGTTGTGTAAGTTTCACATTTAACTACCTCAGCTACTGT	5	0.125	No Hit
TGGAGAACCGTGTGCATCACAGGAAGAAGAAGGGCGGATGCCACCATTGT	5	0.125	No Hit
AGGAATATATTCCATCCAGGTATAGTGACAAGAGGGTATGTGGTTCAATG	5	0.125	No Hit
GGGAATGTGAAAGCTGGTAAAGCACTTGGGTTAGAGACCGCCAGGCCTCT	5	0.125	No Hit
GAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACTAGGTTC	5	0.125	No Hit
TGAACTCTTGAGCTGATTCATCTCCATATTATAAATGTATTTTAAATGTT	5	0.125	No Hit
ACATTCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAA	5	0.125	No Hit
TCTTCTCTATCTATAGATGAGAAGCCTGGAAAGCAGACTTTCTTTATATT	5	0.125	No Hit
AGAGAGCTGAACAGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAG	5	0.125	No Hit
CGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCAC	5	0.125	No Hit
GATTTGGTCTAGTTTTGGGTGGGTTTGGTATGACTAGCAGGAGGTGGTGG	5	0.125	No Hit
GCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAACGTG	5	0.125	No Hit
TCTTCTTTGAAGCCAAACAGTACTCTGGATTTAAAGCTAAGACAAATATG	5	0.125	No Hit
AGATATATCACCCGGACATCACGGTGCCTGGCCTCGGATGCAAACCTGCC	5	0.125	No Hit
TTGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAAC	5	0.125	No Hit
AGCATGACTGTAGCATAGAAAATTATGTGACTCCTCAAAAATATCTACAG	5	0.125	No Hit
TCAGCTCCCTAACTTCAACTGTACATAGTATTCTGATGCATAGTCGGTGA	5	0.125	No Hit
AGAGATATATAGATATACATATTCCAACCCCACATAACCAGACCTTCCTT	5	0.125	No Hit
TCTGTAGCATAGAAAATTATGTGACTCCTCAAAAATATCTACAGTGCTAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.075	0.0
76-77	0.0	0.0	0.0	0.075	0.0
78-79	0.0	0.0	0.0	0.075	0.0
80-81	0.0	0.0	0.0	0.075	0.0
82-83	0.0	0.0	0.0	0.2	0.0
84-85	0.0	0.0	0.0	0.275	0.0
86-87	0.0	0.0	0.0	0.4625	0.0
88-89	0.0	0.0	0.0	0.5875	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
Read 11269947 spots for SRR32569730.sra
Written 11269947 spots for SRR32569730.sra
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
Read 11269946 spots for SRR32569730.sra
Written 11269946 spots for SRR32569730.sra
SRR ids: ['SRR32569730.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_pob71w5c
SRR32569730.sra spots: 90159569
blocks: [[1, 11269946], [11269947, 22539892], [22539893, 33809838], [33809839, 45079784], [45079785, 56349730], [56349731, 67619676], [67619677, 78889622], [78889623, 90159569]]
SRR32569730 file size 21813836
SRR32569730 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32569730 SRR32569730_1.fastq SRR32569730_2.fastq
Input file:	SRR32569730_1.fastq
Paired file:	SRR32569730_2.fastq
trimmed:	SRR32569730-trimmed-pair1.fastq, SRR32569730-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 05:45:55 2026 >> started

Tue Mar 31 05:48:20 2026 >> done (145.778s)
90159569 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
90159569 (100.00%) read pairs available; of these:
    8375 ( 0.01%) trimmed read pairs available after processing
90151194 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	       1	  0.00%
 95	       1	  0.00%
 96	       1	  0.00%
 97	       2	  0.00%
 98	      72	  0.00%
 99	     416	  0.00%
100	    7882	  0.01%
101	90151194	 99.99%
90159569 reads passed initial QC


criterion=sequence-density
sequence-density=10.37
sequence-density-rank=1
fanout-score=2.12
fanout-score-rank=9
prefix-density=11.24
prefix-fanout=2.0
sequence=TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGAT


criterion=fanout-score
sequence-density=0.21
sequence-density-rank=29
fanout-score=58.06
fanout-score-rank=1
prefix-density=6.14
prefix-fanout=2.0
sequence=AGTGAAATTCCT


criterion=sequence-density
sequence-density=2.00
sequence-density-rank=1
fanout-score=3.85
fanout-score-rank=21
prefix-density=7.47
prefix-fanout=1.0
sequence=TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAGACTGTC


criterion=fanout-score
sequence-density=0.17
sequence-density-rank=26
fanout-score=76.16
fanout-score-rank=1
prefix-density=7.49
prefix-fanout=1.7
sequence=ATTGGATCAACTAATCTCCTAGG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGAT -y TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAGACTGTC -o SRR32569730 SRR32569730_1.fastq SRR32569730_2.fastq
Input file:	SRR32569730_1.fastq
Paired file:	SRR32569730_2.fastq
trimmed:	SRR32569730-trimmed-pair1.fastq, SRR32569730-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTG
-- paired 3' end adapter sequence (-y):	TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 05:58:17 2026 >> started

Tue Mar 31 06:02:29 2026 >> done (251.833s)
64399692 read pairs processed; of these:
      78 ( 0.00%) short read pairs filtered out after trimming by size control
     585 ( 0.00%) empty read pairs filtered out after trimming by size control
64399029 (100.00%) read pairs available; of these:
     317 ( 0.00%) trimmed read pairs available after processing
64398712 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	       4	  0.00%
 95	       2	  0.00%
 96	       1	  0.00%
 97	       8	  0.00%
 98	      86	  0.00%
 99	     362	  0.00%
100	    5809	  0.01%
101	64392757	 99.99%


criterion=sequence-density
sequence-density=1.68
sequence-density-rank=1
fanout-score=60.52
fanout-score-rank=2
prefix-density=2.18
prefix-fanout=46.7
sequence=ACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCACGACATATCTCGTATGCCGTCTTCTGCTTGAA


criterion=fanout-score
sequence-density=0.07
sequence-density-rank=7
fanout-score=100.56
fanout-score-rank=1
prefix-density=0.43
prefix-fanout=17.0
sequence=CTCCTCCTCCTCG


criterion=sequence-density
sequence-density=1.68
sequence-density-rank=1
fanout-score=41.61
fanout-score-rank=3
prefix-density=2.22
prefix-fanout=31.4
sequence=ACTGTCTCTTATACACATCTGACGCTGCCGACGACCGCATACGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAC


criterion=fanout-score
sequence-density=0.10
sequence-density-rank=6
fanout-score=302.49
fanout-score-rank=1
prefix-density=1.01
prefix-fanout=29.8
sequence=CACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAATCGAACCCCCTAAAATTGGTT
SRR32569730 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 06:07:57
                             Started mapping on |	Mar 31 06:07:58
                                    Finished on |	Mar 31 06:18:47
       Mapping speed, Million of reads per hour |	500.11

                          Number of input reads |	90158906
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	82680241
                        Uniquely mapped reads % |	91.71%
                          Average mapped length |	198.38
                       Number of splices: Total |	47236068
            Number of splices: Annotated (sjdb) |	46786252
                       Number of splices: GT/AG |	46735624
                       Number of splices: GC/AG |	390627
                       Number of splices: AT/AC |	51315
               Number of splices: Non-canonical |	58502
                      Mismatch rate per base, % |	0.48%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.85
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4557275
             % of reads mapped to multiple loci |	5.05%
        Number of reads mapped to too many loci |	150921
             % of reads mapped to too many loci |	0.17%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.06%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2921390	2921390	2921390
N_multimapping	4557275	4557275	4557275
N_noFeature	5701093	79450076	6302985
N_ambiguous	3670187	199604	1075157
UnstrandedReadsAssigned:73308961 PositiveStrandReadsAssigned:3030561 NegativeStrandReadsAssigned:75302099
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32569730 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32569730-trimmed-pair1.fastq
                             SRR32569730-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 90,158,906 reads, 79,790,010 reads pseudoaligned
[quant] estimated average fragment length: 188.172
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,321 rounds

 109283 SRR32569730.ke.tsv
  52636 SRR32569730.se.tsv
 161919 total
==> SRR32569730.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	9	0	0
ENSMUST00000196221.1	9	2	0	0
ENSMUST00000179664.1	11	4	0	0
ENSMUST00000178537.1	12	5	0	0
ENSMUST00000178862.1	14	7	0	0
ENSMUST00000179520.1	11	4	0	0
ENSMUST00000179883.1	16	9	0	0
ENSMUST00000195858.1	10	3	0	0
ENSMUST00000179932.1	12	5	0	0

==> SRR32569730.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	2993
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	0
ENSMUSG00000103025	3
ENSMUSG00000089699	0
ENSMUSG00000103201	4
ENSMUSG00000103147	0
SRR32569730 completed mapping pipeline successfully
