Starting /dee2/code/volunteer_pipeline.sh SRR32569736
    current disk space = 744049492
    free memory = 189592616 
SRR32569736 SRAfilesize
65f564e9d2b88ff6b2425c00d613fcfe  SRR32569736.sra
SRR32569736.sra file validated
SRR32569736 is paired end
SRR32569736 is conventional basespace
SRR32569736 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.587	26.0	12.0	34.0	12.0	34.0
2	32.7825	34.0	34.0	34.0	34.0	34.0
3	33.1435	34.0	34.0	34.0	34.0	34.0
4	33.0895	34.0	34.0	34.0	34.0	34.0
5	33.3195	34.0	34.0	34.0	34.0	34.0
6	33.189	34.0	34.0	34.0	34.0	34.0
7	33.022	34.0	34.0	34.0	34.0	34.0
8	33.081	34.0	34.0	34.0	34.0	34.0
9	33.0985	34.0	34.0	34.0	34.0	34.0
10-11	33.02025	34.0	34.0	34.0	34.0	34.0
12-13	33.066500000000005	34.0	34.0	34.0	34.0	34.0
14-15	32.998	34.0	34.0	34.0	34.0	34.0
16-17	33.12675	34.0	34.0	34.0	34.0	34.0
18-19	33.028999999999996	34.0	34.0	34.0	34.0	34.0
20-21	33.037	34.0	34.0	34.0	34.0	34.0
22-23	32.98325	34.0	34.0	34.0	34.0	34.0
24-25	32.94	34.0	34.0	34.0	34.0	34.0
26-27	33.02825	34.0	34.0	34.0	34.0	34.0
28-29	33.01175	34.0	34.0	34.0	34.0	34.0
30-31	33.128	34.0	34.0	34.0	34.0	34.0
32-33	32.98325	34.0	34.0	34.0	34.0	34.0
34-35	33.0145	34.0	34.0	34.0	34.0	34.0
36-37	32.912000000000006	34.0	34.0	34.0	34.0	34.0
38-39	32.90775	34.0	34.0	34.0	34.0	34.0
40-41	33.02475	34.0	34.0	34.0	34.0	34.0
42-43	32.93275	34.0	34.0	34.0	34.0	34.0
44-45	32.889	34.0	34.0	34.0	34.0	34.0
46-47	32.783	34.0	34.0	34.0	34.0	34.0
48-49	32.73075	34.0	34.0	34.0	34.0	34.0
50-51	32.604	34.0	34.0	34.0	34.0	34.0
52-53	32.577	34.0	34.0	34.0	30.0	34.0
54-55	32.474999999999994	34.0	34.0	34.0	26.0	34.0
56-57	32.52275	34.0	34.0	34.0	26.0	34.0
58-59	32.63825	34.0	34.0	34.0	34.0	34.0
60-61	32.6085	34.0	34.0	34.0	30.0	34.0
62-63	32.464749999999995	34.0	34.0	34.0	26.0	34.0
64-65	32.52425	34.0	34.0	34.0	30.0	34.0
66-67	32.399	34.0	34.0	34.0	26.0	34.0
68-69	32.461749999999995	34.0	34.0	34.0	30.0	34.0
70-71	32.51125	34.0	34.0	34.0	26.0	34.0
72-73	32.39175	34.0	34.0	34.0	26.0	34.0
74-75	32.32275	34.0	34.0	34.0	26.0	34.0
76-77	32.19925	34.0	34.0	34.0	26.0	34.0
78-79	32.244	34.0	34.0	34.0	26.0	34.0
80-81	32.2285	34.0	34.0	34.0	26.0	34.0
82-83	32.2405	34.0	34.0	34.0	26.0	34.0
84-85	32.23825	34.0	34.0	34.0	26.0	34.0
86-87	32.1965	34.0	34.0	34.0	26.0	34.0
88-89	31.99075	34.0	34.0	34.0	26.0	34.0
90-91	32.1065	34.0	34.0	34.0	26.0	34.0
92-93	31.945	34.0	34.0	34.0	26.0	34.0
94-95	31.9975	34.0	34.0	34.0	26.0	34.0
96-97	32.073	34.0	34.0	34.0	26.0	34.0
98-99	32.016999999999996	34.0	34.0	34.0	26.0	34.0
100-101	31.836	34.0	34.0	34.0	26.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	5.0
25	11.0
26	18.0
27	33.0
28	87.0
29	108.0
30	221.0
31	391.0
32	1109.0
33	1996.0
34	21.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	1.0250000000000001	54.525	43.45	1.0
2	39.300000000000004	12.85	10.35	37.5
3	24.9	16.275000000000002	34.150000000000006	24.675
4	23.075000000000003	20.625	28.199999999999996	28.1
5	20.525	29.975	26.474999999999998	23.025000000000002
6	23.25	31.324999999999996	29.425	16.0
7	23.674999999999997	33.074999999999996	27.375	15.875
8	16.75	26.650000000000002	44.425	12.174999999999999
9	18.875	28.799999999999997	36.075	16.25
10-11	16.0625	32.85	37.724999999999994	13.362499999999999
12-13	20.974999999999998	27.037499999999998	32.4375	19.55
14-15	17.2375	27.3125	37.35	18.099999999999998
16-17	18.5	27.875	37.0375	16.5875
18-19	22.0875	26.4625	35.5875	15.862499999999999
20-21	20.9875	28.5625	32.9375	17.5125
22-23	18.6125	31.087500000000002	33.7375	16.5625
24-25	22.575	28.4	30.9625	18.0625
26-27	20.625	30.337500000000002	30.1875	18.85
28-29	19.6125	28.65	32.375	19.3625
30-31	19.7125	29.0875	32.7	18.5
32-33	18.8375	30.5	33.1875	17.474999999999998
34-35	15.987499999999999	31.912499999999998	33.137499999999996	18.9625
36-37	17.175	30.9625	33.5875	18.275
38-39	16.45	30.3875	34.9	18.2625
40-41	19.5875	28.525	34.0	17.8875
42-43	20.5625	30.349999999999998	31.662499999999998	17.424999999999997
44-45	19.4625	33.225	28.875	18.4375
46-47	20.4875	31.412499999999998	31.874999999999996	16.225
48-49	19.662499999999998	32.05	31.6875	16.6
50-51	17.837500000000002	32.237500000000004	31.525	18.4
52-53	17.05	30.175	33.825	18.95
54-55	18.875	33.2375	30.8	17.0875
56-57	17.3875	32.775	32.275	17.5625
58-59	15.6	32.525	31.5625	20.3125
60-61	17.8	33.900000000000006	28.487499999999997	19.8125
62-63	15.075	35.5	31.4625	17.962500000000002
64-65	18.0	33.1125	31.137500000000003	17.75
66-67	17.599999999999998	32.9375	33.525	15.937499999999998
68-69	17.837500000000002	30.337500000000002	32.85	18.975
70-71	15.0	32.625	31.525	20.849999999999998
72-73	16.7625	31.35	31.137500000000003	20.75
74-75	16.7	32.9875	32.8625	17.45
76-77	17.2	31.137500000000003	34.4	17.2625
78-79	16.7875	33.300000000000004	33.775	16.1375
80-81	17.599999999999998	31.125000000000004	35.1625	16.1125
82-83	17.224999999999998	33.9375	31.5125	17.325
84-85	19.025	29.037499999999998	34.887499999999996	17.05
86-87	19.8625	29.525000000000002	34.175	16.4375
88-89	19.9875	29.062500000000004	34.125	16.825000000000003
90-91	21.15	31.15	30.925000000000004	16.775000000000002
92-93	17.3875	32.0125	32.5	18.099999999999998
94-95	18.5	31.0	33.074999999999996	17.424999999999997
96-97	20.349999999999998	30.825000000000003	32.1625	16.662499999999998
98-99	18.6	31.2875	32.6125	17.5
100-101	18.1875	32.4125	31.525	17.875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.5
17	18.5
18	19.5
19	8.5
20	19.0
21	20.5
22	16.5
23	13.0
24	10.5
25	20.0
26	39.0
27	69.0
28	94.5
29	110.5
30	177.5
31	279.5
32	294.5
33	241.0
34	236.5
35	202.0
36	133.5
37	128.0
38	152.0
39	163.0
40	137.5
41	189.0
42	244.0
43	172.0
44	119.5
45	135.5
46	110.5
47	64.5
48	62.5
49	82.0
50	72.0
51	54.0
52	46.5
53	25.5
54	9.5
55	3.0
56	0.5
57	0.5
58	0.5
59	0.5
60	0.5
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.72727272727273	27.3
2	18.649350649350648	17.95
3	10.077922077922077	14.549999999999999
4	6.025974025974026	11.600000000000001
5	2.8051948051948052	6.75
6	1.9740259740259742	5.7
7	1.87012987012987	6.3
8	0.3116883116883117	1.2
9	0.4675324675324675	2.025
>10	1.090909090909091	6.625
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	18	0.44999999999999996	No Hit
AGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	16	0.4	No Hit
AGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	16	0.4	No Hit
AGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	15	0.375	No Hit
ACCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	15	0.375	No Hit
TGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGA	14	0.35000000000000003	No Hit
TGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	13	0.325	No Hit
AGGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATAT	13	0.325	No Hit
AGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGC	13	0.325	No Hit
TCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	12	0.3	No Hit
ACTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	12	0.3	No Hit
AGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	12	0.3	No Hit
TCCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	12	0.3	No Hit
ACCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	11	0.27499999999999997	No Hit
ACCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	11	0.27499999999999997	No Hit
ACATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGC	11	0.27499999999999997	No Hit
AGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACC	11	0.27499999999999997	No Hit
TCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	10	0.25	No Hit
AGTCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGG	10	0.25	No Hit
AGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTGG	10	0.25	No Hit
TGTTCTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGT	10	0.25	No Hit
ACAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTT	9	0.22499999999999998	No Hit
AGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTT	9	0.22499999999999998	No Hit
TCCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTT	9	0.22499999999999998	No Hit
TGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATAT	9	0.22499999999999998	No Hit
ACCCTACTTAGTTTAGTCATTTAGGCAAGGAAATAGACATTGTTTTGAAA	9	0.22499999999999998	No Hit
TGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	9	0.22499999999999998	No Hit
AGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	9	0.22499999999999998	No Hit
TGGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGAT	9	0.22499999999999998	No Hit
ACCAGGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACACA	9	0.22499999999999998	No Hit
AGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATC	8	0.2	No Hit
AGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCAT	8	0.2	No Hit
ACTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGAAGATAGTCATC	8	0.2	No Hit
ACCTTATAACACACTGTGAGGTAGGTGAAGAAATATCCTCACTCTTTTAT	8	0.2	No Hit
AGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAG	8	0.2	No Hit
TGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTGG	8	0.2	No Hit
ACTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTTT	7	0.17500000000000002	No Hit
AAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTCAAA	7	0.17500000000000002	No Hit
TCTCATCTTAATAATACCTGTTACTTATTGTCCAGCAGAGGATCAGTTGG	7	0.17500000000000002	No Hit
AGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTG	7	0.17500000000000002	No Hit
ATAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGATA	7	0.17500000000000002	No Hit
ACACAAAATAACCATTTGGAATTTGCAGTGGAATAATTTGTTAATTGTGA	7	0.17500000000000002	No Hit
ACGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGG	7	0.17500000000000002	No Hit
ACCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTT	7	0.17500000000000002	No Hit
AGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAA	7	0.17500000000000002	No Hit
ACTCCATGTTCCTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAG	7	0.17500000000000002	No Hit
TGTTTATTTTTATGATAAAAAGGATATACTTTTGTATTTTGGGGTAGAAA	7	0.17500000000000002	No Hit
ACTTCTGTTGCAAAGATGTTGTGTTAGAAATGGTATAAAAACCCAGTTAA	7	0.17500000000000002	No Hit
AGTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGA	7	0.17500000000000002	No Hit
TGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGT	7	0.17500000000000002	No Hit
TTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGTAA	7	0.17500000000000002	No Hit
ACCCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGT	7	0.17500000000000002	No Hit
TGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	7	0.17500000000000002	No Hit
AAGCACTTGGAGTCACTGCCCAGGGCAGCAGCCTCCAACCAAGAAAGACA	7	0.17500000000000002	No Hit
AGGCATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTTC	7	0.17500000000000002	No Hit
AGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGGGGACTCTA	7	0.17500000000000002	No Hit
AGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGGTAATATATCAGT	7	0.17500000000000002	No Hit
ACCTGCACACACCTTATTTCTGAAATGGAGAGAGAAACTCAAGAATGTCA	7	0.17500000000000002	No Hit
TCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGCA	7	0.17500000000000002	No Hit
TCTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCA	7	0.17500000000000002	No Hit
TGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCAC	7	0.17500000000000002	No Hit
AGCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTAT	7	0.17500000000000002	No Hit
TCTACCATCTCTCCACATTTTAAAGCCTTTGTGGAGCTCACATCTGTGTA	7	0.17500000000000002	No Hit
AGGTTTTTCTAATACACTTTTATTTTCATGGAGGGCTCTAGAGGATTCCA	7	0.17500000000000002	No Hit
TGCCACATCATTCACATATTTCCCCTACTGTTAGCACAGTTCAATGGTGC	7	0.17500000000000002	No Hit
ACACCATTCCACATTCTTCTGTTGCAAAGATGTTGTGTTAGAAATGGTAT	7	0.17500000000000002	No Hit
AAGGGATTAGAAAGGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGT	7	0.17500000000000002	No Hit
TGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATT	7	0.17500000000000002	No Hit
AGTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAA	7	0.17500000000000002	No Hit
TCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTT	7	0.17500000000000002	No Hit
TCCCTGCTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	7	0.17500000000000002	No Hit
TCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGC	7	0.17500000000000002	No Hit
AGGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGAT	6	0.15	No Hit
ATAGAGATAGAGTAGGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTT	6	0.15	No Hit
TCCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCA	6	0.15	No Hit
AACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAA	6	0.15	No Hit
ACTAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGAT	6	0.15	No Hit
TATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAACA	6	0.15	No Hit
ACTCATTTTTAACAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTT	6	0.15	No Hit
ATGGGTATGATTGCTGTTAGCCTTTCTGTTTTAATATTAGCTAGACATTC	6	0.15	No Hit
TGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	6	0.15	No Hit
ATGGCATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTT	6	0.15	No Hit
TGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATTGGGGACTCTA	6	0.15	No Hit
TGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	6	0.15	No Hit
AGGGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATT	6	0.15	No Hit
TGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	6	0.15	No Hit
TCCGATGTTTATTTTTATGATAAAAAGGATATACTTTTGTATTTTGGGGT	6	0.15	No Hit
TGCAAGGAAATAGACATTGTTTTGAAATAGTTGTACAGGATCTTCGCTGA	6	0.15	No Hit
ACCCACTTACTGTTCTTACATGACCTGACTATCCTAACAAACTCTATTCT	6	0.15	No Hit
AGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAA	6	0.15	No Hit
TGTGCATTCTTATTTTATAATATTGACTCTATGACTCAAAAATTACAAGT	6	0.15	No Hit
ACTAGGTTTTTCTAATACACTTTTATTTTCATGGAGGGCTCTAGAGGATT	6	0.15	No Hit
AGGACTCTATGACTCAAAAATTACAAGTGTTTATAACCCTACTTAGTTTA	6	0.15	No Hit
AGGCTAACTATGCTATTCTCCACTGCGCCCCATTGATCAGACAATGGCTT	6	0.15	No Hit
ACCTTATTTCTGAAATGGAGAGAGAAACTCAAGAATGTCACCGACTATGC	6	0.15	No Hit
AGGAAAAAATGAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGA	6	0.15	No Hit
TCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCTT	6	0.15	No Hit
ACTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTAAAGCCTTT	6	0.15	No Hit
AGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	6	0.15	No Hit
ACACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGT	6	0.15	No Hit
ACCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGA	6	0.15	No Hit
ACTTCTGGTTTGTCTTCATCTACCAGCTATTACTCCTCTTTTGCTCAGAG	6	0.15	No Hit
AGGAGTCACATAATTTTCTATGCTACAGTCATGCTTCAGAAAGTCTAATG	6	0.15	No Hit
TCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGG	6	0.15	No Hit
TGGAAAAAATGAGGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGA	6	0.15	No Hit
AAGACTTCAACACACCACTTTCATCAATGGACAGATCATGGAAACAGAAA	6	0.15	No Hit
ACCTTTCTGTTTTAATATTAGCTAGACATTCTCTCAATAACACACAGATG	6	0.15	No Hit
ACTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGCTTTACCA	6	0.15	No Hit
AGGCAGTTGTATTTACAAATAATCACATATATGATGCTTACAGAATGATG	6	0.15	No Hit
ACCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	6	0.15	No Hit
TGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	5	0.125	No Hit
ACAGTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAA	5	0.125	No Hit
TGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATATG	5	0.125	No Hit
TCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	5	0.125	No Hit
TGTCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGG	5	0.125	No Hit
ATCTCATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTT	5	0.125	No Hit
TGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAA	5	0.125	No Hit
TTGTCTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAAC	5	0.125	No Hit
TCTCAGAGATAAATTTAAAATACAAACATTGGAAAGAGGAAGGAGAAAAG	5	0.125	No Hit
TCCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	5	0.125	No Hit
TGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGGT	5	0.125	No Hit
TCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATG	5	0.125	No Hit
TGGCATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTTC	5	0.125	No Hit
AGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTGTG	5	0.125	No Hit
TGTTACTTATTGTCCAGCAGAGGATCAGTTGGCCTCACCAGGGCTGAATT	5	0.125	No Hit
TCTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTG	5	0.125	No Hit
AGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAATTT	5	0.125	No Hit
ACTGTACCATTCTCTTCCATTGAGAATTCTAAGCCATTATGTGGAGGAGC	5	0.125	No Hit
TCCCCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATG	5	0.125	No Hit
AGCCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAG	5	0.125	No Hit
ACTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTTTTCTCTTTGTGTCTG	5	0.125	No Hit
ACTATGACTCAAAAATTACAAGTGTTTATAACCCTACTTAGTTTAGTCAT	5	0.125	No Hit
AACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAGC	5	0.125	No Hit
AGGTTTTGTGAAGGTAATATATCAGTAATATGGTTGACATAAATAAGCTG	5	0.125	No Hit
AGCCACAGTCTGTGTTCTGGGTAGTGTCCAAAATAGTTAACGTACCAGGT	5	0.125	No Hit
AGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAA	5	0.125	No Hit
ACTGCTTTCCAGGCTTCTCATCTATAGATAGAGAAGACTTTTACAAGGTG	5	0.125	No Hit
AACCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCAT	5	0.125	No Hit
AGTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCA	5	0.125	No Hit
TCTCATTTTTAACAGCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTT	5	0.125	No Hit
TTGGCATAGTTGCTGCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTT	5	0.125	No Hit
AATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAACA	5	0.125	No Hit
TATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATATGA	5	0.125	No Hit
AGTCTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACC	5	0.125	No Hit
ACCTAAATGAACTGTTGGACATCAGGTTCATGAAAACGGTTTTGTGAAGG	5	0.125	No Hit
ACTCTCCACCACCTCCTGCTAGTCATACCAAACCCACCCAAAACTAGACC	5	0.125	No Hit
AGTGGCATCCGCCCTTCTTCTTCCTGTGATGCACACGGTTCTCCATGTTC	5	0.125	No Hit
ACTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATATGA	5	0.125	No Hit
AGTCACATAATTTTCTATGCTACAGTCATGCTTCAGAAAGTCTAATGTCG	5	0.125	No Hit
TAGTTATGAAAGATTGAGGGAAAGTGATAAAGAACAAAAGGGAGGGATAT	5	0.125	No Hit
TCCTGCCTAGACTTTTAATGGAAAGTTTTCACTGGCATAGTTGCTGCTTT	5	0.125	No Hit
ACAGGAATATTCAAATTACCTTTTACCCACTTACTGTTCTTACATGACCT	5	0.125	No Hit
AAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAGCAGCCTCCAACCAAGA	5	0.125	No Hit
ACTGTGTCTTTCATTTGCCTATGAAAAGAATGTTAGTTGGCTGTAACCAT	5	0.125	No Hit
AGCCAATTTGAAAATTTAAAACTTTGATTGAGTCCATTGACAACTTAATT	5	0.125	No Hit
TTAAATGTGAAACTTACACAACTTAAAATAATGAGAGAATGGAGAACCAA	5	0.125	No Hit
AGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATATATG	5	0.125	No Hit
TGGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTTA	5	0.125	No Hit
AGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTGCTTGGAAGCAA	5	0.125	No Hit
TGTACTGAATGGAGCCACATCATTCACATATTTCCCCTACTGTTAGCACA	5	0.125	No Hit
TGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACC	5	0.125	No Hit
TAGGGATTAGAAAGGGCCAGGTTGTCTAGATGTATTTATAAACATTTAGT	5	0.125	No Hit
AGGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTTCTTTGTTGCTATC	5	0.125	No Hit
ACCACATTCTTCTGTTGCAAAGATGTTGTGTTAGAAATGGTATAAAAACC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.1625	0.0
76-77	0.0	0.0	0.0	0.2375	0.0
78-79	0.0	0.0	0.0	0.275	0.0
80-81	0.0	0.0	0.0	0.325	0.0
82-83	0.0	0.0	0.0	0.55	0.0
84-85	0.0	0.0	0.0	0.7	0.0
86-87	0.0	0.0	0.0	0.8875	0.0
88-89	0.0	0.0	0.0	1.0499999999999998	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32569736 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32569736_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	36
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	19.057	12.0	12.0	26.0	12.0	34.0
2	31.903	34.0	34.0	34.0	26.0	34.0
3	32.3595	34.0	34.0	34.0	26.0	34.0
4	31.6655	34.0	34.0	34.0	26.0	34.0
5	32.3385	34.0	34.0	34.0	26.0	34.0
6	31.833	34.0	34.0	34.0	26.0	34.0
7	31.791	34.0	34.0	34.0	26.0	34.0
8	32.224	34.0	34.0	34.0	26.0	34.0
9	32.2705	34.0	34.0	34.0	26.0	34.0
10-11	32.194500000000005	34.0	34.0	34.0	26.0	34.0
12-13	32.34125	34.0	34.0	34.0	26.0	34.0
14-15	32.25775	34.0	34.0	34.0	26.0	34.0
16-17	32.327749999999995	34.0	34.0	34.0	26.0	34.0
18-19	32.346000000000004	34.0	34.0	34.0	26.0	34.0
20-21	32.156000000000006	34.0	34.0	34.0	26.0	34.0
22-23	32.187250000000006	34.0	34.0	34.0	26.0	34.0
24-25	32.171499999999995	34.0	34.0	34.0	26.0	34.0
26-27	32.38875	34.0	34.0	34.0	26.0	34.0
28-29	32.36425	34.0	34.0	34.0	26.0	34.0
30-31	32.38	34.0	34.0	34.0	26.0	34.0
32-33	32.501999999999995	34.0	34.0	34.0	30.0	34.0
34-35	32.04725	34.0	34.0	34.0	26.0	34.0
36-37	32.462	34.0	34.0	34.0	26.0	34.0
38-39	32.473749999999995	34.0	34.0	34.0	26.0	34.0
40-41	32.330749999999995	34.0	34.0	34.0	26.0	34.0
42-43	32.082499999999996	34.0	34.0	34.0	26.0	34.0
44-45	32.32125	34.0	34.0	34.0	26.0	34.0
46-47	32.2245	34.0	34.0	34.0	26.0	34.0
48-49	32.15675	34.0	34.0	34.0	26.0	34.0
50-51	32.2435	34.0	34.0	34.0	26.0	34.0
52-53	32.084500000000006	34.0	34.0	34.0	26.0	34.0
54-55	31.986250000000002	34.0	34.0	34.0	26.0	34.0
56-57	32.094750000000005	34.0	34.0	34.0	26.0	34.0
58-59	31.83375	34.0	34.0	34.0	26.0	34.0
60-61	32.004999999999995	34.0	34.0	34.0	26.0	34.0
62-63	32.012	34.0	34.0	34.0	26.0	34.0
64-65	32.051500000000004	34.0	34.0	34.0	26.0	34.0
66-67	31.82175	34.0	34.0	34.0	26.0	34.0
68-69	31.826	34.0	34.0	34.0	26.0	34.0
70-71	31.719	34.0	34.0	34.0	26.0	34.0
72-73	31.81475	34.0	34.0	34.0	26.0	34.0
74-75	31.503	34.0	34.0	34.0	26.0	34.0
76-77	31.58125	34.0	34.0	34.0	26.0	34.0
78-79	31.555500000000002	34.0	34.0	34.0	26.0	34.0
80-81	31.603	34.0	34.0	34.0	26.0	34.0
82-83	31.435499999999998	34.0	34.0	34.0	26.0	34.0
84-85	31.358249999999998	34.0	34.0	34.0	26.0	34.0
86-87	31.322	34.0	34.0	34.0	26.0	34.0
88-89	31.31475	34.0	34.0	34.0	26.0	34.0
90-91	31.18925	34.0	34.0	34.0	26.0	34.0
92-93	31.235500000000002	34.0	34.0	34.0	26.0	34.0
94-95	30.94425	34.0	34.0	34.0	12.0	34.0
96-97	30.68225	34.0	34.0	34.0	12.0	34.0
98-99	31.05975	34.0	34.0	34.0	19.0	34.0
100-101	30.5735	34.0	34.0	34.0	12.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	4.0
23	5.0
24	22.0
25	35.0
26	50.0
27	80.0
28	148.0
29	216.0
30	365.0
31	668.0
32	1400.0
33	1003.0
34	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.900000000000002	33.050000000000004	29.099999999999998	14.95
2	28.969849246231156	29.623115577889447	15.42713567839196	25.979899497487434
3	22.425	37.974999999999994	28.000000000000004	11.600000000000001
4	16.025	33.875	33.275	16.825000000000003
5	17.775	40.949999999999996	24.5	16.775000000000002
6	17.45	38.45	26.775	17.325
7	16.05	42.0	25.525	16.425
8	14.099999999999998	23.849999999999998	43.5	18.55
9	17.125	29.45	30.875000000000004	22.55
10-11	18.1625	35.7625	27.675	18.4
12-13	20.3	29.012500000000003	27.8375	22.85
14-15	16.9375	31.05	31.874999999999996	20.1375
16-17	17.2	31.137500000000003	31.412499999999998	20.25
18-19	18.462500000000002	33.0875	29.7125	18.7375
20-21	18.475	34.2875	30.062499999999996	17.175
22-23	17.625	32.475	29.5	20.4
24-25	17.175	34.4	29.462500000000002	18.9625
26-27	17.8	33.25	30.5125	18.4375
28-29	18.1625	32.2125	30.837500000000002	18.787499999999998
30-31	17.6125	34.1	29.2	19.0875
32-33	18.8875	33.025	28.6125	19.475
34-35	18.774478837650033	31.358180669614654	32.43209096651927	17.435249526216047
36-37	18.8125	31.637500000000003	31.412499999999998	18.1375
38-39	18.1125	32.375	29.425	20.0875
40-41	18.475	33.85	29.099999999999998	18.575
42-43	15.2375	33.725	30.562499999999996	20.474999999999998
44-45	17.175	33.037499999999994	28.712500000000002	21.075
46-47	18.3	31.674999999999997	31.05	18.975
48-49	16.9125	32.5125	33.125	17.45
50-51	16.3625	33.2625	30.875000000000004	19.5
52-53	17.0875	32.0125	31.674999999999997	19.225
54-55	18.05	31.95	30.4	19.6
56-57	17.0	33.6	29.4	20.0
58-59	17.3875	31.3125	31.2625	20.0375
60-61	18.1875	32.025	30.775000000000002	19.0125
62-63	18.5375	31.175000000000004	30.1875	20.1
64-65	17.1	33.125	30.075000000000003	19.7
66-67	16.75	33.5	31.0625	18.6875
68-69	18.725	32.574999999999996	30.0375	18.6625
70-71	16.900000000000002	32.75	31.337500000000002	19.0125
72-73	17.25	32.875	32.475	17.4
74-75	18.637500000000003	31.874999999999996	31.175000000000004	18.3125
76-77	16.3875	31.474999999999998	31.85	20.2875
78-79	15.982478097622026	32.052565707133915	31.401752190237797	20.56320400500626
80-81	17.3875	30.587500000000002	31.974999999999998	20.05
82-83	16.775000000000002	31.624999999999996	32.225	19.375
84-85	17.8875	32.324999999999996	31.2875	18.5
86-87	15.8	34.325	30.6375	19.2375
88-89	16.8	32.574999999999996	31.387500000000003	19.2375
90-91	18.175	32.4	31.0375	18.387500000000003
92-93	17.2375	32.824999999999996	30.662499999999998	19.275000000000002
94-95	18.212500000000002	32.475	29.625	19.6875
96-97	17.585335018963338	31.76991150442478	32.37673830594184	18.268015170670036
98-99	16.55	30.612499999999997	33.6	19.2375
100-101	16.8375	33.225	31.2875	18.65
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	2.0
18	4.5
19	4.0
20	5.0
21	9.0
22	9.0
23	5.5
24	15.5
25	34.0
26	55.0
27	81.5
28	111.5
29	150.5
30	186.5
31	191.0
32	196.5
33	211.5
34	218.5
35	220.5
36	198.5
37	184.0
38	180.0
39	186.0
40	176.5
41	182.5
42	209.5
43	190.5
44	179.0
45	155.0
46	92.5
47	65.0
48	67.5
49	68.0
50	55.5
51	38.5
52	30.5
53	18.5
54	7.0
55	2.0
56	0.5
57	0.5
58	0.5
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.0625
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.125
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	1.125
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.92500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0923510750793	52.55
2	17.025026436376454	24.15
3	5.322523792738808	11.325000000000001
4	2.2559041240747266	6.4
5	0.5639760310186817	2.0
6	0.42298202326401124	1.7999999999999998
7	0.10574550581600281	0.525
8	0.14099400775467041	0.8
9	0.07049700387733521	0.44999999999999996
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGAAAAACAACCTCAGCGAAGATCCTGTACAACTATTTCAAAACAATGT	9	0.22499999999999998	No Hit
TTGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAAC	9	0.22499999999999998	No Hit
GTGTAGAGTCCCCAATTCAGCCCTGGTGAGGCCAACTGATCCTCTGCTGG	8	0.2	No Hit
AGGAAAAACAACCTCAGCGAAGATCCTGTACAACTATTTCAAAACAATGT	8	0.2	No Hit
AGTCTTCTCTATCTATAGATGAGAAGCCTGGAAAGCAGACTTTCTTTATA	8	0.2	No Hit
AGAGCTGAACAGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAGAG	8	0.2	No Hit
TCAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATTCT	7	0.17500000000000002	No Hit
CATTTGGTCTAGTTTTGGGTGGGTTTGGTATGACTAGCAGGAGGTGGTGG	7	0.17500000000000002	No Hit
AGTGATTATTTAGAAATGAGCCCATCATTCTGTAAGCATCATATATGTGA	7	0.17500000000000002	No Hit
TGAGCTGAACAGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAGAG	6	0.15	No Hit
TCATTCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAA	6	0.15	No Hit
TCACGGTGCCTGGCCTCGGATGCAAACCTGCCAGTCCTGTCTCTTACTTT	6	0.15	No Hit
AATTTCCCAGCTTATTTATGTCAACCATATTACTGATATATTACCTTCAC	6	0.15	No Hit
TGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTAT	6	0.15	No Hit
AAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATTTGCT	6	0.15	No Hit
AGGAGAACCGTGTGCATCACAGGAAGAAGAAGGGCGGATGCCACCATTGT	6	0.15	No Hit
CTGTAAATACAACTGCCAATTTTATGGTTACAGCCAACTAACATTCTTTT	6	0.15	No Hit
ACTCCTTCTGAAACCAATATTTTTTCTACCCCAAAATACAAAAGTATATC	6	0.15	No Hit
AGGAATACTTACAGTATTGACACCCAATATAATCTCCTCTGATTCTCTAA	6	0.15	No Hit
ATGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAAC	6	0.15	No Hit
ACCTCTTTCCAATGTTTGTATTTTAAATTTATCTCTGAGCAAAAGAGGAG	6	0.15	No Hit
AGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATTTGCTA	5	0.125	No Hit
AAACCAGACCTTCCTTTAGAATTCAACTTGTAAGTGTGTCTTTCTTGGTT	5	0.125	No Hit
TGGAGAGATGGTAGGAAAAACAACCTCAGCGAAGATCCTGTACAACTATT	5	0.125	No Hit
AGAGAGACTTTCTATTCTCAGTAAGAGAACCAGAGATGACTATCTTCTTT	5	0.125	No Hit
ATGGACACCAAGTGTTTCCATAGATGGACCATTTTCTACTTAAATACACT	5	0.125	No Hit
AGGAATCTGTACACACCTGGGTCCCCATCCCTACTCTATCTCTATACCTC	5	0.125	No Hit
TGGCAAATGAAAGACACAGACACAAAGAGAAAAATCTTCTACTTCCTAAA	5	0.125	No Hit
AATGGCATCCAAAAGAACAAATGTTTTTCTGGGTATGAAACCATCATACT	5	0.125	No Hit
TCTTCTTTGAAGCCAAACAGTACTCTGGATTTAAAGCTAAGACAAATATG	5	0.125	No Hit
GACATAATATTGCTGGGGGGAAAAAGAGTTGAGTCTCTTTTCAGTGGTTT	5	0.125	No Hit
AGTCATGTTGACACTGGCTGAACTAATGGCATCCAAAAGAACAAATGTTT	5	0.125	No Hit
AAGAGACTGTACATATTTGCTTAATTTGCCTTAAATATTTTGTGCTTTTC	5	0.125	No Hit
TGCCAACTAACATTCTTTTCATAGGCAAATGAAAGACACAGACACAAAGA	5	0.125	No Hit
TCACAGATGTGAGCTCCACAAAGGCTTTAAAATGTGGAGAGATGGTAGGA	5	0.125	No Hit
ACAATTTTCAAAAAAAATTAAAACAACACAAGCAACCCAACTCACACCAG	5	0.125	No Hit
ACCTCTCACTAAATTGCAGGAACATGGAGAACCGTGTGCATCACAGGAAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.1625	0.0
76-77	0.0	0.0	0.0	0.2375	0.0
78-79	0.0	0.0	0.0	0.275	0.0
80-81	0.0	0.0	0.0	0.325	0.0
82-83	0.0	0.0	0.0	0.575	0.0
84-85	0.0	0.0	0.0	0.7250000000000001	0.0
86-87	0.0	0.0	0.0	0.9125000000000001	0.0
88-89	0.0	0.0	0.0	1.0625	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998422 spots for SRR32569736.sra
Written 10998422 spots for SRR32569736.sra
Read 10998425 spots for SRR32569736.sra
Written 10998425 spots for SRR32569736.sra
SRR ids: ['SRR32569736.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gajwk55k
SRR32569736.sra spots: 87987379
blocks: [[1, 10998422], [10998423, 21996844], [21996845, 32995266], [32995267, 43993688], [43993689, 54992110], [54992111, 65990532], [65990533, 76988954], [76988955, 87987379]]
SRR32569736 file size 21287776
SRR32569736 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32569736 SRR32569736_1.fastq SRR32569736_2.fastq
Input file:	SRR32569736_1.fastq
Paired file:	SRR32569736_2.fastq
trimmed:	SRR32569736-trimmed-pair1.fastq, SRR32569736-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 06:01:26 2026 >> started

Tue Mar 31 06:05:16 2026 >> done (230.638s)
87987379 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
87987379 (100.00%) read pairs available; of these:
    6053 ( 0.01%) trimmed read pairs available after processing
87981326 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	       1	  0.00%
 95	       2	  0.00%
 96	       5	  0.00%
 97	       2	  0.00%
 98	      73	  0.00%
 99	     451	  0.00%
100	    5519	  0.01%
101	87981326	 99.99%
87987379 reads passed initial QC


criterion=sequence-density
sequence-density=9.74
sequence-density-rank=1
fanout-score=2.10
fanout-score-rank=10
prefix-density=10.54
prefix-fanout=1.9
sequence=TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGAT


criterion=fanout-score
sequence-density=0.19
sequence-density-rank=35
fanout-score=58.53
fanout-score-rank=1
prefix-density=5.81
prefix-fanout=1.9
sequence=AGTGAAATTCCT


criterion=sequence-density
sequence-density=1.68
sequence-density-rank=1
fanout-score=4.30
fanout-score-rank=19
prefix-density=7.01
prefix-fanout=1.0
sequence=TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAGACTGTC


criterion=fanout-score
sequence-density=0.16
sequence-density-rank=28
fanout-score=59.69
fanout-score-rank=1
prefix-density=8.96
prefix-fanout=1.1
sequence=ATTCCCACTGGGACCTTCACCAA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGTATCTAGTTGTGGCATATCACTATGGAGAT -y TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGATTTGAAGCCGCAGCATGATACTGACATTTTGTAGACGTAGTCTGACTTTTCCTATACGTCTCCATTTATTGATGAGACTGTC -o SRR32569736 SRR32569736_1.fastq SRR32569736_2.fastq
Input file:	SRR32569736_1.fastq
Paired file:	SRR32569736_2.fastq
trimmed:	SRR32569736-trimmed-pair1.fastq, SRR32569736-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTG
-- paired 3' end adapter sequence (-y):	TTGGATCAACATTCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 06:10:04 2026 >> started

Tue Mar 31 06:13:15 2026 >> done (191.664s)
58658253 read pairs processed; of these:
     128 ( 0.00%) short read pairs filtered out after trimming by size control
     702 ( 0.00%) empty read pairs filtered out after trimming by size control
58657423 (100.00%) read pairs available; of these:
     267 ( 0.00%) trimmed read pairs available after processing
58657156 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 94	       4	  0.00%
 95	       2	  0.00%
 96	       5	  0.00%
 97	       4	  0.00%
 98	      77	  0.00%
 99	     335	  0.00%
100	    3922	  0.01%
101	58653074	 99.99%


criterion=sequence-density
sequence-density=1.45
sequence-density-rank=1
fanout-score=57.49
fanout-score-rank=3
prefix-density=1.85
prefix-fanout=45.0
sequence=ACTGTCTCTTATACACATCTCCGAGCCCACGAGACTATTGCGCTCATCT


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=12
fanout-score=79.03
fanout-score-rank=1
prefix-density=0.29
prefix-fanout=16.7
sequence=AAAACAAAACAAA


criterion=sequence-density
sequence-density=1.48
sequence-density-rank=1
fanout-score=38.78
fanout-score-rank=3
prefix-density=1.91
prefix-fanout=30.0
sequence=ACTGTCTCTTATACACATCTGACGCTGCCGACGACCTAACACAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA


criterion=fanout-score
sequence-density=0.09
sequence-density-rank=6
fanout-score=261.42
fanout-score-rank=1
prefix-density=0.89
prefix-fanout=27.2
sequence=CACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAATCGAACCCCCTAAAATTGGTT
SRR32569736 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 06:15:33
                             Started mapping on |	Mar 31 06:15:33
                                    Finished on |	Mar 31 06:25:27
       Mapping speed, Million of reads per hour |	533.25

                          Number of input reads |	87986549
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	80120802
                        Uniquely mapped reads % |	91.06%
                          Average mapped length |	198.55
                       Number of splices: Total |	46420915
            Number of splices: Annotated (sjdb) |	45965201
                       Number of splices: GT/AG |	45930706
                       Number of splices: GC/AG |	378086
                       Number of splices: AT/AC |	50033
               Number of splices: Non-canonical |	62090
                      Mismatch rate per base, % |	0.45%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.79
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4360427
             % of reads mapped to multiple loci |	4.96%
        Number of reads mapped to too many loci |	154438
             % of reads mapped to too many loci |	0.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.79%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3505320	3505320	3505320
N_multimapping	4360427	4360427	4360427
N_noFeature	5089241	77067291	5620090
N_ambiguous	3533630	140306	1043382
UnstrandedReadsAssigned:71497931 PositiveStrandReadsAssigned:2913205 NegativeStrandReadsAssigned:73457330
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32569736 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32569736-trimmed-pair1.fastq
                             SRR32569736-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 87,986,549 reads, 77,821,524 reads pseudoaligned
[quant] estimated average fragment length: 194.634
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,306 rounds

 109283 SRR32569736.ke.tsv
  52636 SRR32569736.se.tsv
 161919 total
==> SRR32569736.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	3.5	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	1	0	0
ENSMUST00000178862.1	14	3	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	3.5	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	1	0	0

==> SRR32569736.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	3092
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	3
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	2
SRR32569736 completed mapping pipeline successfully
