Starting /dee2/code/volunteer_pipeline.sh SRR32570304
    current disk space = 859869680
    free memory = 197893776 
SRR32570304 SRAfilesize
e9e535a662c78e62c40668ed97b4e9e6  SRR32570304.sra
SRR32570304.sra file validated
SRR32570304 is paired end
SRR32570304 is conventional basespace
SRR32570304 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32570304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9965	34.0	34.0	34.0	34.0	34.0
2	33.2775	34.0	34.0	34.0	34.0	34.0
3	33.2205	34.0	34.0	34.0	34.0	34.0
4	33.3085	34.0	34.0	34.0	34.0	34.0
5	33.468	34.0	34.0	34.0	34.0	34.0
6	33.404	34.0	34.0	34.0	34.0	34.0
7	33.3905	34.0	34.0	34.0	34.0	34.0
8	33.4495	34.0	34.0	34.0	34.0	34.0
9	33.549	34.0	34.0	34.0	34.0	34.0
10-11	33.47425	34.0	34.0	34.0	34.0	34.0
12-13	33.48125	34.0	34.0	34.0	34.0	34.0
14-15	33.530249999999995	34.0	34.0	34.0	34.0	34.0
16-17	33.455	34.0	34.0	34.0	34.0	34.0
18-19	33.473	34.0	34.0	34.0	34.0	34.0
20-21	33.5465	34.0	34.0	34.0	34.0	34.0
22-23	33.54875	34.0	34.0	34.0	34.0	34.0
24-25	33.52875	34.0	34.0	34.0	34.0	34.0
26-27	33.5625	34.0	34.0	34.0	34.0	34.0
28-29	33.580749999999995	34.0	34.0	34.0	34.0	34.0
30-31	33.573499999999996	34.0	34.0	34.0	34.0	34.0
32-33	33.501999999999995	34.0	34.0	34.0	34.0	34.0
34-35	33.553749999999994	34.0	34.0	34.0	34.0	34.0
36-37	33.50325	34.0	34.0	34.0	34.0	34.0
38-39	33.5165	34.0	34.0	34.0	34.0	34.0
40-41	33.532	34.0	34.0	34.0	34.0	34.0
42-43	33.58725	34.0	34.0	34.0	34.0	34.0
44-45	33.53125	34.0	34.0	34.0	34.0	34.0
46-47	33.6045	34.0	34.0	34.0	34.0	34.0
48-49	33.4995	34.0	34.0	34.0	34.0	34.0
50-51	33.4925	34.0	34.0	34.0	34.0	34.0
52-53	33.468500000000006	34.0	34.0	34.0	34.0	34.0
54-55	33.52475	34.0	34.0	34.0	34.0	34.0
56-57	33.51175	34.0	34.0	34.0	34.0	34.0
58-59	33.534499999999994	34.0	34.0	34.0	34.0	34.0
60-61	33.466750000000005	34.0	34.0	34.0	34.0	34.0
62-63	33.3185	34.0	34.0	34.0	34.0	34.0
64-65	33.479	34.0	34.0	34.0	34.0	34.0
66-67	33.54575	34.0	34.0	34.0	34.0	34.0
68-69	33.50325	34.0	34.0	34.0	34.0	34.0
70-71	33.4785	34.0	34.0	34.0	34.0	34.0
72-73	33.445750000000004	34.0	34.0	34.0	34.0	34.0
74-75	33.45575	34.0	34.0	34.0	34.0	34.0
76-77	33.468	34.0	34.0	34.0	34.0	34.0
78-79	33.42725	34.0	34.0	34.0	34.0	34.0
80-81	33.5475	34.0	34.0	34.0	34.0	34.0
82-83	33.48425	34.0	34.0	34.0	34.0	34.0
84-85	33.4005	34.0	34.0	34.0	34.0	34.0
86-87	33.46675	34.0	34.0	34.0	34.0	34.0
88-89	33.44	34.0	34.0	34.0	34.0	34.0
90-91	33.45375	34.0	34.0	34.0	34.0	34.0
92-93	33.417	34.0	34.0	34.0	34.0	34.0
94-95	33.47825	34.0	34.0	34.0	34.0	34.0
96-97	33.3745	34.0	34.0	34.0	34.0	34.0
98-99	33.33825	34.0	34.0	34.0	34.0	34.0
100-101	33.2115	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	9.0
27	19.0
28	25.0
29	40.0
30	82.0
31	84.0
32	232.0
33	2627.0
34	879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.2	14.674999999999999	7.249999999999999	25.874999999999996
2	24.825	12.875	32.25	30.049999999999997
3	23.1	20.849999999999998	26.875	29.175
4	22.475	26.775	27.224999999999998	23.525
5	26.75	31.275	24.3	17.675
6	24.474999999999998	32.875	23.775	18.875
7	17.8	28.325	39.574999999999996	14.299999999999999
8	19.3	27.925	33.425	19.35
9	18.9	26.575	32.775	21.75
10-11	20.1	34.275	28.5625	17.0625
12-13	21.15	29.1375	30.6375	19.075
14-15	20.8	28.1125	29.7	21.3875
16-17	22.4875	27.987499999999997	29.7125	19.8125
18-19	21.732598898347522	27.904356534802204	30.045067601402103	20.317976965448175
20-21	20.1125	29.262500000000003	29.6625	20.962500000000002
22-23	20.7875	29.612500000000004	29.4125	20.1875
24-25	21.85	28.8875	28.812500000000004	20.45
26-27	19.55	27.9375	30.862499999999997	21.65
28-29	20.2625	26.5625	30.2375	22.9375
30-31	20.200000000000003	28.537499999999998	30.2125	21.05
32-33	20.12042147516307	28.399397892624183	29.929754139488207	21.550426492724537
34-35	20.7	30.925000000000004	29.975	18.4
36-37	20.9875	27.3875	29.6875	21.9375
38-39	20.71383844708829	28.57858484658735	31.18346900438322	19.52410770194114
40-41	20.175	28.825	29.475	21.525
42-43	21.2625	28.95	28.449999999999996	21.337500000000002
44-45	21.7	29.475	28.000000000000004	20.825
46-47	21.725	27.8875	30.0875	20.3
48-49	22.3125	28.5625	28.625	20.5
50-51	20.1	30.925000000000004	28.762500000000003	20.2125
52-53	20.125	29.725	29.762499999999996	20.3875
54-55	21.1625	28.675	28.9875	21.175
56-57	20.16522718738265	27.95093253223182	31.493303292026535	20.390536988358992
58-59	21.512500000000003	26.787499999999998	29.862499999999997	21.837500000000002
60-61	20.607727897961738	27.410278854570464	30.97411529323496	21.007877954232836
62-63	21.159638554216865	28.614457831325304	29.27961847389558	20.94628514056225
64-65	21.286447253159803	27.643599048930046	29.796020523088472	21.273933174821675
66-67	20.0875	28.5875	29.475	21.85
68-69	22.53345004376641	26.15980992872327	28.810804051519316	22.495935975990996
70-71	19.787499999999998	28.1375	30.225	21.85
72-73	18.276207155366524	27.633224918689013	31.41105829372029	22.679509632224168
74-75	20.9	26.85	30.775000000000002	21.475
76-77	21.099999999999998	29.425	29.062500000000004	20.4125
78-79	21.45	29.025000000000002	29.475	20.05
80-81	23.1875	27.675	29.012500000000003	20.125
82-83	20.9375	28.812500000000004	29.912499999999998	20.3375
84-85	22.12611257364924	28.657389996239186	29.221511846558855	19.99498558355271
86-87	21.912499999999998	28.9375	28.212500000000002	20.9375
88-89	22.369573376704615	27.899411985487298	29.400725634930563	20.330289002877517
90-91	21.725	28.525	28.375	21.375
92-93	20.8875	29.4	28.3375	21.375
94-95	21.5	29.1375	29.1125	20.25
96-97	22.021796317173994	27.59614180132782	28.836277088813727	21.545784792684454
98-99	20.9375	30.0	27.787499999999998	21.275
100-101	22.830306825297434	29.016906700062616	27.551659361302445	20.60112711333751
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	0.5
17	1.5
18	18.0
19	18.0
20	4.5
21	10.5
22	10.5
23	7.0
24	9.5
25	13.5
26	25.5
27	35.0
28	34.5
29	39.5
30	50.0
31	73.0
32	93.5
33	80.5
34	87.0
35	105.5
36	116.5
37	112.0
38	111.0
39	153.5
40	163.0
41	179.5
42	217.5
43	219.0
44	224.0
45	204.5
46	174.5
47	192.0
48	196.0
49	165.5
50	165.0
51	194.5
52	158.0
53	87.5
54	55.5
55	45.0
56	39.5
57	26.0
58	18.0
59	19.0
60	15.0
61	5.5
62	3.0
63	5.0
64	4.5
65	3.5
66	2.0
67	2.5
68	3.0
69	1.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.15
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.35000000000000003
34-35	0.0
36-37	0.0
38-39	0.1875
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.13749999999999998
58-59	0.0
60-61	0.0375
62-63	0.4
64-65	0.11249999999999999
66-67	0.0
68-69	0.0375
70-71	0.0
72-73	0.075
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.2875
86-87	0.0
88-89	0.08750000000000001
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.21250000000000002
98-99	0.0
100-101	0.1875
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.315789473684205	29.425
2	22.63157894736842	23.65
3	9.85645933014354	15.45
4	5.4066985645933014	11.3
5	2.200956937799043	5.75
6	1.4354066985645932	4.5
7	0.9090909090909091	3.325
8	0.28708133971291866	1.2
9	0.43062200956937796	2.025
>10	0.5263157894736842	3.375
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCT	17	0.42500000000000004	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	14	0.35000000000000003	No Hit
GCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGATGGGGGTGTCGAT	14	0.35000000000000003	No Hit
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	13	0.325	No Hit
GGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAA	12	0.3	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	12	0.3	No Hit
GTTGCAATAAAATAAAAAAAAAATCTACAAAATTCAAATATATAAAATTT	12	0.3	No Hit
CCCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTT	11	0.27499999999999997	No Hit
GCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCT	10	0.25	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	10	0.25	No Hit
GTACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCTCTCCACATTTTA	10	0.25	No Hit
CCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAA	9	0.22499999999999998	No Hit
AGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCC	9	0.22499999999999998	No Hit
GCCATTATGTGGAGGAGCTGTTCAGCTCTCTCTAACCTATTTAGATTTTA	9	0.22499999999999998	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	9	0.22499999999999998	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	9	0.22499999999999998	No Hit
GTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGAG	9	0.22499999999999998	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	9	0.22499999999999998	No Hit
GAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACA	9	0.22499999999999998	No Hit
GCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAA	9	0.22499999999999998	No Hit
CAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAA	8	0.2	No Hit
GTCCACTTCATTGATGCTACTGGTTTGCAAAGGAGAGAACAGTTTATATA	8	0.2	No Hit
GCAAGATTGAAGTGTCTGTTGGATTAATTAACTGCAATTCATCTCCAGTA	8	0.2	No Hit
GGCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAG	8	0.2	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	8	0.2	No Hit
GTACAATGTTTACAAAGTAAGAGACAGGACTGGCAGGTTTGCATCCGAGG	8	0.2	No Hit
GCATATTTGTCTTAGCTTTAAATCCAGAGTACTGTTTGGCTTCAAAGAAG	7	0.17500000000000002	No Hit
GCCAAAATACACTTTTCCTTTTCAAGTCCACTCTAACCGGCTTAAGTGAG	7	0.17500000000000002	No Hit
CTTCATTTCATCACAAATTGTAACTTACTTAACTGACCAAAAAAACTATG	7	0.17500000000000002	No Hit
CCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGACT	7	0.17500000000000002	No Hit
AGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAGAA	7	0.17500000000000002	No Hit
GTTCTCTTCTGGTTTGTCTTCATCTACCAGCTATTACTCCTCTTTTGCTC	7	0.17500000000000002	No Hit
GGTTGACATAAATAAGCTGGGAAATAGTTCTTTTAGGAAGTAGAAGATTT	7	0.17500000000000002	No Hit
GGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGAG	7	0.17500000000000002	No Hit
GGATGCTTACAGAATGATGGGCTCATTTCTAAATAATCACATATGCTAAA	7	0.17500000000000002	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	7	0.17500000000000002	No Hit
CCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	7	0.17500000000000002	No Hit
GGGATAGTATAAAACCAGGCAAGGAAGTTTAAGACATCTTCCACTGTGGT	7	0.17500000000000002	No Hit
GGGAGTGTTTGCACTGTGTTTAGAAATCCCTCAAAAGGTTTGCATAGCCA	7	0.17500000000000002	No Hit
GTTCCTCTCTGCCTTTCTCCTTTATGGCCTCTGCCACACTTCCTACCTCT	7	0.17500000000000002	No Hit
ATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCT	7	0.17500000000000002	No Hit
CCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGT	7	0.17500000000000002	No Hit
GTTCTTTTTTAGTAGTGAAGAGAGTAGAACAGAAAATTAATGAACTTCTC	7	0.17500000000000002	No Hit
CCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTC	7	0.17500000000000002	No Hit
GGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAG	7	0.17500000000000002	No Hit
TGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTT	6	0.15	No Hit
GGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGAT	6	0.15	No Hit
GGGGTGTCGATGATCCTCTTGTCACCGTATTTCTTCCACAGGCCTCTGCT	6	0.15	No Hit
GTCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAG	6	0.15	No Hit
CAGAATACTATGTACAGTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTG	6	0.15	No Hit
GTCATCTCTGGTTCTCTTACTGAGAATAGAAAGTCTCTCCTTACTGAGAA	6	0.15	No Hit
ATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTA	6	0.15	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	6	0.15	No Hit
TTTTCAATGGCAGCAATGGCTAATTCTGAAGCCAGCTGCACTTCAATATT	6	0.15	No Hit
CTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTC	6	0.15	No Hit
GTGGGCTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAA	6	0.15	No Hit
CTGAGGTAGTTAAATGTGAAACTTACACAACTTAAAATAATGAGAGAATG	6	0.15	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	6	0.15	No Hit
GGCAGGACTTTCTGGCTGCCAGCCCAGAATCAAGTTGAATGAGAGAAACT	6	0.15	No Hit
GCTGCTTGTATGACCAACTTCTTGAATTTTCATAGGGATATTTGCATTTA	6	0.15	No Hit
GCACAGAATTTCTAGACTTCTTGGGTCATAGAAGGCTGTAGTAACAACAC	6	0.15	No Hit
GGAAGTGGCAAGCAAGCTTTAACCAGTGTGTGTGTGTGTGTGTGTGTGTG	6	0.15	No Hit
GTTCATTTAAGGAAATTGATTTCTATGCAGACAGGCTATGGTCAAAGTTC	6	0.15	No Hit
GTGGGGTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAA	6	0.15	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	6	0.15	No Hit
CTGCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGCACTTG	6	0.15	No Hit
GTTGAATTCTAAAGGAAGGTCTGGTTATGTGGGGTTGGAATATGTATATC	6	0.15	No Hit
GTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCA	6	0.15	No Hit
GGGGCATCAAGGAAGTTGAATGCAGGGCCTTCCATAATTCTGGCACAAAT	6	0.15	No Hit
GCTGCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGATGGGGGTGTC	6	0.15	No Hit
GCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCAC	6	0.15	No Hit
GTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCT	6	0.15	No Hit
GCTCATTTCTAAATAATCACATATGCTAAAAGCTAGGTTTTTCTAATACA	6	0.15	No Hit
CCACTGAAAAGAGACTCAACTCTTTTTCCCCCCAGCAATATTATGTTGCT	6	0.15	No Hit
TGCAGTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAA	6	0.15	No Hit
GGCCTCTGCCACACTTCCTACCTCTTCTCCGATCTCTTGGACGTCTTTCC	5	0.125	No Hit
AGTAGCTTTGACTAATTATTTTTCAGTCTGTTTAATTGGATAATGGTCAG	5	0.125	No Hit
GAAATGGAGAGAGAAACTCAAGAATGTCACCGACTATGCATCAGAATACT	5	0.125	No Hit
GTATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	5	0.125	No Hit
GGTCACCAAAGCCTCCTGCACGAGGCAAGGCAATAGCTTTCCTTGGGCTA	5	0.125	No Hit
GTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAAT	5	0.125	No Hit
TGAAGAGTAAGGCCATGGTAACCCTGAATCTCATTGTAAGTAAAAGAGAA	5	0.125	No Hit
GCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCA	5	0.125	No Hit
GTGTTCATGAATGATATTGGTTTGAAGTTCTTTAGCCCCATATTATCTTG	5	0.125	No Hit
TCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCA	5	0.125	No Hit
CAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTT	5	0.125	No Hit
CCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	5	0.125	No Hit
GCTTGTGTTGTTTTAATTTTTTTTGAAAATTGTTTTGCACAAAATAACCA	5	0.125	No Hit
GGAAGTCAGAAGAGTGCATCAAGTGCCCTGGAATTGGGAAAACAGATGGT	5	0.125	No Hit
AGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAAT	5	0.125	No Hit
GTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCACAATACTACATA	5	0.125	No Hit
GCCAGCCAGCAGACCAGAAATAGTAAGATCAACTGCCCAAGATTACCAGG	5	0.125	No Hit
GCCATCTGGAAGCAGGCTCACCAAGTATGATGGTTTCATACCCAGAAAAA	5	0.125	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	5	0.125	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	5	0.125	No Hit
CCCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATC	5	0.125	No Hit
GTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTATATATA	5	0.125	No Hit
GGGAAAGTGATAAAGAACAAAAGGGAGGGATATTATTCTCTCATTTTTAA	5	0.125	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	5	0.125	No Hit
GGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAATCCATATTTTGG	5	0.125	No Hit
CCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTA	5	0.125	No Hit
GAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTA	5	0.125	No Hit
CTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCG	5	0.125	No Hit
CATTTAAATTTTTGTTTCAAATAAAATTCCAAATACATTTGTACAATGTT	5	0.125	No Hit
GCTAGAGCACTTGGAGTCACTGCCCAGGGCAGCAGCCTCCAACCAAGAAA	5	0.125	No Hit
GGCACGTGGTTTCCGGAATCATACCTGCTGACCCATGTCATTCTGCACCT	5	0.125	No Hit
GCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGCACTTGGA	5	0.125	No Hit
GAACACTACAGGGGCCACTAATTGAGAAGAACTATCAGAGGGAAGTTTTT	5	0.125	No Hit
GCCAGGCACTCTATTTGAGTAGATCAACATCTTTGCCAGCCAGCAGACCA	5	0.125	No Hit
ACACTTACAAGTTGAATTCTAAAGGAAGGTCTGGTTATGTGGGGTTGGAA	5	0.125	No Hit
GCGGACATATAGTAAGTCTTGGCAGCTGAGTTTATAACCTGGTCAATAGC	5	0.125	No Hit
CCCCATTTCATGTCCACAGACATCCAGCCCCTCAAGAAATAGCACAGAAC	5	0.125	No Hit
CTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAA	5	0.125	No Hit
CCGGGTTGCACATTGGCCAGCTGGAATGTAGAAAAAACACTGGGATTTTT	5	0.125	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	5	0.125	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	5	0.125	No Hit
GACCAAATTTTTATTTTACCCTCCATTTGGCAGATGGTGAAACTGAGGCA	5	0.125	No Hit
GCCACATTATGTTATTCTTGGCTCTGAATGTAGAATTGTACATGATCACA	5	0.125	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	5	0.125	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	5	0.125	No Hit
GCCTAGACTTTTAATGGAAAGTTTTCACTGGCATAGTTGCTGCTTTTCAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.1125	0.0	0.0	0.0	0.0
74-75	0.1625	0.0	0.0	0.0	0.0
76-77	0.21250000000000002	0.0	0.0	0.0	0.0
78-79	0.2625	0.0	0.0	0.0	0.0
80-81	0.42500000000000004	0.0	0.0	0.0	0.0
82-83	0.4625	0.0	0.0	0.0	0.0
84-85	0.5	0.0	0.0	0.0	0.0
86-87	0.6375	0.0	0.0	0.0	0.0
88-89	0.725	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32570304 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32570304_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.1985	34.0	34.0	34.0	34.0	34.0
2	33.229	34.0	34.0	34.0	34.0	34.0
3	32.991	34.0	34.0	34.0	34.0	34.0
4	33.226	34.0	34.0	34.0	34.0	34.0
5	33.215	34.0	34.0	34.0	34.0	34.0
6	33.252	34.0	34.0	34.0	34.0	34.0
7	33.2845	34.0	34.0	34.0	34.0	34.0
8	33.33	34.0	34.0	34.0	34.0	34.0
9	33.349	34.0	34.0	34.0	34.0	34.0
10-11	33.355000000000004	34.0	34.0	34.0	34.0	34.0
12-13	33.38775	34.0	34.0	34.0	34.0	34.0
14-15	33.345749999999995	34.0	34.0	34.0	34.0	34.0
16-17	33.27775	34.0	34.0	34.0	34.0	34.0
18-19	33.265249999999995	34.0	34.0	34.0	34.0	34.0
20-21	33.3475	34.0	34.0	34.0	34.0	34.0
22-23	33.3445	34.0	34.0	34.0	34.0	34.0
24-25	33.28725	34.0	34.0	34.0	34.0	34.0
26-27	33.332499999999996	34.0	34.0	34.0	34.0	34.0
28-29	33.230500000000006	34.0	34.0	34.0	34.0	34.0
30-31	33.402	34.0	34.0	34.0	34.0	34.0
32-33	33.39975	34.0	34.0	34.0	34.0	34.0
34-35	33.29875	34.0	34.0	34.0	34.0	34.0
36-37	33.37025	34.0	34.0	34.0	34.0	34.0
38-39	33.379999999999995	34.0	34.0	34.0	34.0	34.0
40-41	33.278499999999994	34.0	34.0	34.0	34.0	34.0
42-43	33.31225	34.0	34.0	34.0	34.0	34.0
44-45	33.31675	34.0	34.0	34.0	34.0	34.0
46-47	33.3435	34.0	34.0	34.0	34.0	34.0
48-49	33.181	34.0	34.0	34.0	34.0	34.0
50-51	33.32575	34.0	34.0	34.0	34.0	34.0
52-53	33.247	34.0	34.0	34.0	34.0	34.0
54-55	33.335750000000004	34.0	34.0	34.0	34.0	34.0
56-57	33.275	34.0	34.0	34.0	34.0	34.0
58-59	33.314	34.0	34.0	34.0	34.0	34.0
60-61	33.25	34.0	34.0	34.0	34.0	34.0
62-63	33.3445	34.0	34.0	34.0	34.0	34.0
64-65	33.343	34.0	34.0	34.0	34.0	34.0
66-67	33.20825000000001	34.0	34.0	34.0	34.0	34.0
68-69	33.256	34.0	34.0	34.0	34.0	34.0
70-71	33.338750000000005	34.0	34.0	34.0	34.0	34.0
72-73	33.3	34.0	34.0	34.0	34.0	34.0
74-75	33.161249999999995	34.0	34.0	34.0	34.0	34.0
76-77	33.14175	34.0	34.0	34.0	34.0	34.0
78-79	33.195499999999996	34.0	34.0	34.0	34.0	34.0
80-81	33.2395	34.0	34.0	34.0	34.0	34.0
82-83	33.1325	34.0	34.0	34.0	34.0	34.0
84-85	33.191500000000005	34.0	34.0	34.0	34.0	34.0
86-87	33.232	34.0	34.0	34.0	34.0	34.0
88-89	33.1755	34.0	34.0	34.0	34.0	34.0
90-91	33.117999999999995	34.0	34.0	34.0	34.0	34.0
92-93	33.089749999999995	34.0	34.0	34.0	34.0	34.0
94-95	33.1415	34.0	34.0	34.0	34.0	34.0
96-97	33.1075	34.0	34.0	34.0	34.0	34.0
98-99	32.9935	34.0	34.0	34.0	34.0	34.0
100-101	32.929249999999996	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	0.0
21	3.0
22	5.0
23	9.0
24	20.0
25	12.0
26	17.0
27	30.0
28	26.0
29	47.0
30	69.0
31	107.0
32	252.0
33	2835.0
34	566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05	25.15	10.8	20.0
2	21.375	30.95	29.725	17.95
3	20.724999999999998	30.775000000000002	30.55	17.95
4	24.25	34.975	25.1	15.675
5	25.05	37.95	20.0	17.0
6	19.325	37.7	25.3	17.675
7	19.75	24.25	35.6	20.4
8	19.45	27.250000000000004	28.125	25.174999999999997
9	19.775000000000002	27.650000000000002	29.675	22.900000000000002
10-11	22.6	34.5625	22.525000000000002	20.3125
12-13	21.925	28.375	27.975	21.725
14-15	21.4375	28.95	29.099999999999998	20.5125
16-17	21.72172172172172	30.08008008008008	27.214714714714717	20.983483483483482
18-19	21.512500000000003	30.162499999999998	28.575	19.75
20-21	20.4875	31.55	26.474999999999998	21.4875
22-23	22.1375	30.837500000000002	24.6125	22.412499999999998
24-25	21.9	30.0	27.1375	20.962500000000002
26-27	21.6125	29.475	27.3	21.6125
28-29	20.758732941029173	30.03630900212846	26.956303993990232	22.248654062852136
30-31	20.7125	30.2125	27.950000000000003	21.125
32-33	20.7625	29.4875	28.999999999999996	20.75
34-35	23.7625	27.975	27.212500000000002	21.05
36-37	22.8625	28.475	28.6625	20.0
38-39	23.849999999999998	28.8875	27.750000000000004	19.5125
40-41	22.8625	29.1625	27.275	20.7
42-43	21.79134350763072	30.810607955966972	28.471353515136354	18.92669502126595
44-45	22.8	29.1875	25.95	22.0625
46-47	22.9875	29.525000000000002	26.974999999999998	20.5125
48-49	20.56613226452906	29.195891783567134	28.820140280561123	21.417835671342687
50-51	21.925	29.675	26.575	21.825
52-53	21.925	29.8875	27.275	20.9125
54-55	20.6125	31.025000000000002	27.762500000000003	20.599999999999998
56-57	21.525	30.725	27.3875	20.3625
58-59	22.6375	29.062500000000004	27.8375	20.4625
60-61	22.05	28.675	28.237499999999997	21.0375
62-63	21.25	29.725	26.6125	22.412499999999998
64-65	21.2	29.5875	27.150000000000002	22.0625
66-67	21.625	30.2125	26.9625	21.2
68-69	21.3125	29.825000000000003	28.325	20.5375
70-71	22.425	30.312499999999996	26.525	20.7375
72-73	22.3875	30.075000000000003	27.1	20.4375
74-75	19.6375	29.9625	28.7375	21.6625
76-77	20.572002007024587	28.62518815855494	29.453085800301054	21.34972403411942
78-79	21.7875	29.4375	28.6625	20.1125
80-81	21.178383787840882	29.046785088816613	28.74655991993996	21.02827120340255
82-83	21.12376423476411	29.170316606181956	28.45701414090852	21.248905018145415
84-85	20.2625	29.799999999999997	28.275	21.6625
86-87	22.1375	29.349999999999998	28.512500000000003	20.0
88-89	21.361531723188588	31.047428356901513	26.792641721937176	20.79839819797272
90-91	21.863260706235913	29.551715502128722	27.748559979964938	20.836463811670423
92-93	21.16468378209142	31.85973700688792	26.311834690043835	20.66374452097683
94-95	21.762500000000003	29.549999999999997	28.725	19.9625
96-97	20.5875	31.025000000000002	28.075	20.3125
98-99	21.05	31.2125	25.8625	21.875
100-101	20.5875	30.525000000000002	27.287499999999998	21.6
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	1.5
14	0.5
15	0.0
16	1.0
17	3.5
18	3.0
19	1.0
20	4.5
21	8.0
22	6.0
23	6.5
24	9.5
25	12.5
26	20.0
27	22.5
28	24.0
29	38.0
30	45.0
31	57.5
32	76.5
33	88.0
34	97.0
35	89.5
36	93.5
37	105.0
38	120.5
39	175.0
40	214.0
41	225.0
42	240.5
43	243.5
44	220.0
45	193.5
46	202.5
47	228.5
48	233.0
49	174.0
50	117.0
51	120.0
52	122.0
53	102.5
54	75.0
55	42.5
56	21.0
57	19.0
58	17.5
59	12.5
60	18.5
61	15.5
62	3.5
63	6.5
64	7.0
65	3.5
66	4.0
67	4.0
68	1.5
69	1.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.1
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.1625
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.075
44-45	0.0
46-47	0.0
48-49	0.2
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.35000000000000003
78-79	0.0
80-81	0.075
82-83	0.11249999999999999
84-85	0.0
86-87	0.0
88-89	0.11249999999999999
90-91	0.17500000000000002
92-93	0.1875
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.05
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.09125840537944	28.675
2	23.919308357348704	24.9
3	9.798270893371757	15.299999999999999
4	5.3314121037463975	11.1
5	2.6416906820365034	6.875000000000001
6	1.536983669548511	4.8
7	0.5283381364073007	1.925
8	0.33621517771373677	1.4000000000000001
9	0.1440922190201729	0.675
>10	0.6724303554274735	4.35
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	17	0.42500000000000004	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	15	0.375	No Hit
ACGGTGCCTGGCCTCGGATGCAAACCTGCCAGTCCTGTCTCTTACTTTGT	15	0.375	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	13	0.325	No Hit
GCCAGTGTGATGAAGACAAATCATCTCGTGACTGTGGAAGGAGGCTGGCC	13	0.325	No Hit
GGTACCAACAACATGCCATATGCATAGAGCAGCACAGAGCAACATAATAT	12	0.3	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	12	0.3	No Hit
GGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGA	12	0.3	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	12	0.3	No Hit
GTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGC	11	0.27499999999999997	No Hit
GCTATTAATCAAGGTATGGATGAAGAACTAGAAAGAGATGAAAAAGTTTT	11	0.27499999999999997	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	11	0.27499999999999997	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	10	0.25	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	10	0.25	No Hit
CCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGC	9	0.22499999999999998	No Hit
GAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGA	9	0.22499999999999998	No Hit
GTGAAAGATAAAATCTAAATAGGTTAGAGAGAGCTGAACAGCTCCTCCAC	9	0.22499999999999998	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	8	0.2	No Hit
GGGAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCT	8	0.2	No Hit
GAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTG	8	0.2	No Hit
TGAAGAACTAGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTG	8	0.2	No Hit
TTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAGATGAAGACA	8	0.2	No Hit
GGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTG	8	0.2	No Hit
GCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCAT	8	0.2	No Hit
GATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATT	7	0.17500000000000002	No Hit
CAGTGTGATGAAGACAAATCATCTCGTGACTGTGGAAGGAGGCTGGCCAC	7	0.17500000000000002	No Hit
ACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACA	7	0.17500000000000002	No Hit
CTTTATTTGAATTTTTTAAAGCATGGTTTGGGATTTAAAAAGAAAGTCAC	7	0.17500000000000002	No Hit
GAGAAAGGCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAG	7	0.17500000000000002	No Hit
GAGCACTGACTGCTCTTGCAAAGGACCCAGGCTTGAGTAGTTCACTGCAA	7	0.17500000000000002	No Hit
GTCACATCCTCCTGGCACAAAGAGACTGTACATATTTGCTTAATTTGCCT	7	0.17500000000000002	No Hit
GAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACT	7	0.17500000000000002	No Hit
GGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGT	7	0.17500000000000002	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	7	0.17500000000000002	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	7	0.17500000000000002	No Hit
CTTCTCTATCTATAGATGAGAAGCCTGGAAAGCAGACTTTCTTTATATTT	6	0.15	No Hit
GTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGG	6	0.15	No Hit
GGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTT	6	0.15	No Hit
GTGACTTACTCTCACATGCCAGGAAACCAGAACTAGCAAGTTTAAGGAAA	6	0.15	No Hit
GGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATTCTGATGCATAGTC	6	0.15	No Hit
CAATGGGCCCAGATTTGGGCAATCACCAAGTTGTGCTTGTGATGATCCAG	6	0.15	No Hit
CAATGGAAGAGAATGGTACAGGTACCAACAACATGCCATATGCATAGAGC	6	0.15	No Hit
GAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCT	6	0.15	No Hit
GTGTGTGCAGGCAACGTGAAGATATATCACCCGGACATCACGGTGCCTGG	6	0.15	No Hit
TGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATT	6	0.15	No Hit
GGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATC	6	0.15	No Hit
GAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAAATCTAAAT	6	0.15	No Hit
GTGGAGAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAAA	6	0.15	No Hit
AAATAAGGTGTGTGCAGGCAACGTGAAGATATATCACCCGGACATCACGG	6	0.15	No Hit
GTTTGTATTTTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAG	6	0.15	No Hit
GAAACCATCATACTTGGTGAGCCTGCTTCCAGATGGCAAGAGTCTGGGCT	6	0.15	No Hit
AATGAGGCCTCTGGGTCCAGCTGCATTGCTTCAGGCCTTGGAACATCTGT	6	0.15	No Hit
TTTGCCTTAAATATTTTGTGCTTTTCTCCTTCCTCTTTCCAATGTTTGTA	6	0.15	No Hit
GTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACT	6	0.15	No Hit
GCTCATTCCAGAAACTACACTTGAATTTAGACTTTTCCTCAATTTGTTTG	6	0.15	No Hit
GCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATCGGAGAA	6	0.15	No Hit
GGTTTACAGACATCATTCCAGTTAATAACTGCTTGCTGGTTACCATGAAT	6	0.15	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	6	0.15	No Hit
GTTTTATACAGAAAAACTGAAGTTGACTATTTGGTCTAGTTTTGGGTGGG	6	0.15	No Hit
GAATCCCAAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTAT	6	0.15	No Hit
AACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTAC	6	0.15	No Hit
AATGCCTTTCCAGGCTAGAACCCATGTTGTGATCTGCTGGCCGGACACAA	6	0.15	No Hit
GAGAATCTGAGCAGGGGTGCTATCAGAGTATCAAGTGAAGAGGGAAGTAG	6	0.15	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	6	0.15	No Hit
TCCCCCAAAAGGGCACTTTAGCTGTACAACTTGCTCTGAGGCACTTACTG	6	0.15	No Hit
AGAAGCCAGTGTGATGAAGACAAATCATCTCGTGACTGTGGAAGGAGGCT	6	0.15	No Hit
CCAGCCTGGACCTGCTGCGGTCCTTGCCGAGAGTGAGCCTGGCCAACCTG	6	0.15	No Hit
GATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGTGAATAT	5	0.125	No Hit
TATACATATTCCAACCCCACATAACCAGACCTTCCTTTAGAATTCAACTT	5	0.125	No Hit
GGAAAACACTTGTCTCTGGCAAGATCACCTTACATTTAGCAATTTGATGG	5	0.125	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	5	0.125	No Hit
CTACTTCTTAAAAGATCAGCCAATGGATTCCTGGGAGTTTGTATAGCTCA	5	0.125	No Hit
CACAAAGTGAAAGATAAAATCTAAATAGGTTAGAGAGAGCTGAACAGCTC	5	0.125	No Hit
CTGAAATATGGCCCACTCACACTGCTGGCGGGTCTGAAGTGCGGTTGGCC	5	0.125	No Hit
GATCAACTCAACGCGAGTGGACCTGTCATTGACAGAGGGCCTCTCCTCCT	5	0.125	No Hit
AGAAGAGAAGAGAAGAGAAGAGAAACCCTCTCCAGGCTAAGTTGGCTTTC	5	0.125	No Hit
CATCATACTTGGTGAGCCTGCTTCCAGATGGCAAGAGTCTGGGCTCCTTA	5	0.125	No Hit
ATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTG	5	0.125	No Hit
GTATGACTAGCAGGAGGTGGTGGAGAGAACATTCTATAGAGAGATTCTAT	5	0.125	No Hit
GTTTAAGGAAATGCTCAGGAACCTAGTGTAGGAGTTGGTTAACTGTATAA	5	0.125	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	5	0.125	No Hit
GGAGAGAGCTCTGATGTTAGTGTGAGTCCCATGACGGTTGTCTTTCGTGA	5	0.125	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	5	0.125	No Hit
CAGCTCCCTAACTTCAACTGTACATAGTATTCTGATGCATAGTCGGTGAC	5	0.125	No Hit
GTGAATGGAAATTATGGATAGTTCTGGGTAAGTTTCACTGGAGGGTTTGA	5	0.125	No Hit
GCCGGTTAGAGTGGACTTGAAAAGGAAAAGTGTATTTTGGCACAGGGTAA	5	0.125	No Hit
CTTTACTAAATGTTTATAAATACATCTAGACAACCTGGCCCTTTCTAATC	5	0.125	No Hit
ATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAA	5	0.125	No Hit
CACTAAGCTGTGTTTGTCTACCTGGTACGTTAACTATTTTGGACACTACC	5	0.125	No Hit
GAAAAGACTTTGTAAGATGATGCTGTGCTCATGAGGGAGTTATCCCTCTA	5	0.125	No Hit
GGAGAACCGTGTGCATCACAGGAAGAAGAAGGGCGGATGCCACCATTGTC	5	0.125	No Hit
AAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAA	5	0.125	No Hit
GTCTGTTTCCAGAACTGTTTCCAGGTGCAGAATGACATGGGTCAGCAGGT	5	0.125	No Hit
CGGTGCCTGGCCTCGGATGCAAACCTGCCAGTCCTGTCTCTTACTTTGTA	5	0.125	No Hit
AGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAGAGAATGGTACAG	5	0.125	No Hit
GAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAG	5	0.125	No Hit
CTTGTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTGACT	5	0.125	No Hit
GAAAAACTGAAGTTGACTATTTGGTCTAGTTTTGGGTGGGTTTGGTATGA	5	0.125	No Hit
GGTACTTCACATGTCTTCTTATGAAAGGGCAAGGCCTGGCTACCATGGAA	5	0.125	No Hit
CCTGAAGCCCAGTCCTAACTCCAGAAAACGGGAAAGACGTCCAAGAGATC	5	0.125	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	5	0.125	No Hit
GCAAGGCCTGGCTACCATGGAAAGAAGGAGGTCACGTAGGAAGCTTCATG	5	0.125	No Hit
CAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCT	5	0.125	No Hit
CAAGCTACTAGAAGAGAACATGAATCAATTAATGGGAAAGGAGCAGTTGG	5	0.125	No Hit
ATACAAGGTTAGCAGAGGCCTGTGGAAGAAATACGGTGACAAGAGGATCA	5	0.125	No Hit
GGGGTACAACAGTCTTCTCTATCAAGGCTCAGAAATAACTGTGTCCATAA	5	0.125	No Hit
AACTGATGTATGGAGTTGCATTTGAACTTCCTGCAGAAGCTCAGTCAAAA	5	0.125	No Hit
GTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGG	5	0.125	No Hit
GTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAA	5	0.125	No Hit
GGCATCCAAAAGAACAAATGTTTTTCTGGGTATGAAACCATCATACTTGG	5	0.125	No Hit
CACAGATGTGAGCTCCACAAAGGCTTTAAAATGTGGAGAGATGGTAGGAA	5	0.125	No Hit
ACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCAT	5	0.125	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	5	0.125	No Hit
CAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATT	5	0.125	No Hit
GTTTGTTTTTGTTTTTGTTTTTGTTTTTTTCATTTAGATAGCAGGCCTCA	5	0.125	No Hit
GCTGTTAAAAATGAGAGAATAATATCCCTCCCTTTTGTTCTTTATCACTT	5	0.125	No Hit
AAATAAAATAAAAATAAAAAAACAACTCTTGCTTTTCTTCTCTGTAGCCA	5	0.125	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	5	0.125	No Hit
GAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTGGAA	5	0.125	No Hit
GGTGAATCTGAAGGACTGCTTACTCAACACCTCATCTTGGGGCCTGGAGA	5	0.125	No Hit
AAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTC	5	0.125	No Hit
TAAAAAGCAATAAGTCTTGAGTGTAGGTCTGTCCTTCCTGGAATGTGGCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.1125	0.0	0.0	0.0	0.0
74-75	0.1625	0.0	0.0	0.0	0.0
76-77	0.21250000000000002	0.0	0.0	0.0	0.0
78-79	0.2625	0.0	0.0	0.0	0.0
80-81	0.42500000000000004	0.0	0.0	0.0	0.0
82-83	0.4625	0.0	0.0	0.0	0.0
84-85	0.4875	0.0	0.0	0.0	0.0
86-87	0.6125	0.0	0.0	0.0	0.0
88-89	0.7	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 5930203 spots for SRR32570304.sra
Written 5930203 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
Read 5930197 spots for SRR32570304.sra
Written 5930197 spots for SRR32570304.sra
SRR ids: ['SRR32570304.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fbwxurb1
SRR32570304.sra spots: 47441582
blocks: [[1, 5930197], [5930198, 11860394], [11860395, 17790591], [17790592, 23720788], [23720789, 29650985], [29650986, 35581182], [35581183, 41511379], [41511380, 47441582]]
SRR32570304 file size 11468068
SRR32570304 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32570304 SRR32570304_1.fastq SRR32570304_2.fastq
Input file:	SRR32570304_1.fastq
Paired file:	SRR32570304_2.fastq
trimmed:	SRR32570304-trimmed-pair1.fastq, SRR32570304-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 03:30:32 2026 >> started

Tue Mar 31 03:31:28 2026 >> done (55.899s)
47441582 read pairs processed; of these:
     176 ( 0.00%) short read pairs filtered out after trimming by size control
   76095 ( 0.16%) empty read pairs filtered out after trimming by size control
47365311 (99.84%) read pairs available; of these:
 1807658 ( 3.82%) trimmed read pairs available after processing
45557653 (96.18%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      11	  0.00%
 19	       8	  0.00%
 20	      16	  0.00%
 21	      12	  0.00%
 22	      12	  0.00%
 23	      14	  0.00%
 24	      12	  0.00%
 25	      10	  0.00%
 26	      16	  0.00%
 27	      23	  0.00%
 28	      37	  0.00%
 29	      48	  0.00%
 30	      53	  0.00%
 31	      74	  0.00%
 32	      82	  0.00%
 33	      79	  0.00%
 34	      94	  0.00%
 35	     144	  0.00%
 36	     139	  0.00%
 37	     146	  0.00%
 38	     235	  0.00%
 39	     280	  0.00%
 40	     321	  0.00%
 41	     348	  0.00%
 42	     390	  0.00%
 43	     410	  0.00%
 44	     413	  0.00%
 45	     497	  0.00%
 46	     576	  0.00%
 47	     693	  0.00%
 48	     838	  0.00%
 49	     930	  0.00%
 50	    1111	  0.00%
 51	    1195	  0.00%
 52	    1468	  0.00%
 53	    1528	  0.00%
 54	    1474	  0.00%
 55	    1783	  0.00%
 56	    1900	  0.00%
 57	    2224	  0.00%
 58	    2550	  0.01%
 59	    2992	  0.01%
 60	    3541	  0.01%
 61	    3954	  0.01%
 62	    4371	  0.01%
 63	    4779	  0.01%
 64	    5296	  0.01%
 65	    5454	  0.01%
 66	    6096	  0.01%
 67	    6627	  0.01%
 68	    7743	  0.02%
 69	    8774	  0.02%
 70	    9774	  0.02%
 71	   11167	  0.02%
 72	   12513	  0.03%
 73	   13976	  0.03%
 74	   15216	  0.03%
 75	   16796	  0.04%
 76	   18921	  0.04%
 77	   19941	  0.04%
 78	   22579	  0.05%
 79	   24791	  0.05%
 80	   27276	  0.06%
 81	   30499	  0.06%
 82	   34049	  0.07%
 83	   37328	  0.08%
 84	   42101	  0.09%
 85	   45930	  0.10%
 86	   49045	  0.10%
 87	   53425	  0.11%
 88	   58095	  0.12%
 89	   62397	  0.13%
 90	   68403	  0.14%
 91	   74325	  0.16%
 92	   81033	  0.17%
 93	   87709	  0.19%
 94	   95235	  0.20%
 95	  100947	  0.21%
 96	  107458	  0.23%
 97	  116059	  0.25%
 98	  121686	  0.26%
 99	  128740	  0.27%
100	  138423	  0.29%
101	45557653	 96.18%
47365311 reads passed initial QC


criterion=sequence-density
sequence-density=1.18
sequence-density-rank=1
fanout-score=5.33
fanout-score-rank=23
prefix-density=6.07
prefix-fanout=1.0
sequence=GTCGTGGTATTACTGAAAGGCCC


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=23
fanout-score=84.80
fanout-score-rank=1
prefix-density=4.69
prefix-fanout=1.1
sequence=GAGGGAAGAATTTTATGTTTACT


criterion=sequence-density
sequence-density=2.02
sequence-density-rank=1
fanout-score=3.75
fanout-score-rank=22
prefix-density=3.78
prefix-fanout=2.0
sequence=AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=23
fanout-score=58.47
fanout-score-rank=1
prefix-density=0.18
prefix-fanout=7.2
sequence=TGAGGAGGAGGATGGAGATGAAGATGAGGAAGCTGAGGCTCCTACGGGCAAGCGGGTAGCTGAGGATGATGAGGATGACGATGTGGACACCAAGAAGCAGAAGACCGAGGAGGATGACTAGACAGCAAAAGGAAAAATAACCTTACGCACCGTGACCTA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTCGTGGTATTACTGAAAGGCCC -y AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA -o SRR32570304 SRR32570304_1.fastq SRR32570304_2.fastq
Input file:	SRR32570304_1.fastq
Paired file:	SRR32570304_2.fastq
trimmed:	SRR32570304-trimmed-pair1.fastq, SRR32570304-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCGTGGTATTACTGAAAGGCCC
-- paired 3' end adapter sequence (-y):	AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 03:35:58 2026 >> started

Tue Mar 31 03:36:35 2026 >> done (37.328s)
15788437 read pairs processed; of these:
     152 ( 0.00%) short read pairs filtered out after trimming by size control
    1795 ( 0.01%) empty read pairs filtered out after trimming by size control
15786490 (99.99%) read pairs available; of these:
     179 ( 0.00%) trimmed read pairs available after processing
15786311 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       4	  0.00%
 19	       0	  0.00%
 20	       4	  0.00%
 21	       2	  0.00%
 22	       3	  0.00%
 23	       5	  0.00%
 24	       4	  0.00%
 25	       1	  0.00%
 26	       8	  0.00%
 27	       5	  0.00%
 28	       7	  0.00%
 29	      20	  0.00%
 30	      20	  0.00%
 31	      30	  0.00%
 32	      28	  0.00%
 33	      25	  0.00%
 34	      43	  0.00%
 35	      57	  0.00%
 36	      51	  0.00%
 37	      57	  0.00%
 38	      81	  0.00%
 39	      82	  0.00%
 40	     115	  0.00%
 41	     120	  0.00%
 42	     118	  0.00%
 43	     146	  0.00%
 44	     129	  0.00%
 45	     180	  0.00%
 46	     213	  0.00%
 47	     233	  0.00%
 48	     309	  0.00%
 49	     313	  0.00%
 50	     359	  0.00%
 51	     407	  0.00%
 52	     493	  0.00%
 53	     465	  0.00%
 54	     490	  0.00%
 55	     566	  0.00%
 56	     626	  0.00%
 57	     746	  0.00%
 58	     798	  0.01%
 59	     958	  0.01%
 60	    1212	  0.01%
 61	    1381	  0.01%
 62	    1441	  0.01%
 63	    1612	  0.01%
 64	    1695	  0.01%
 65	    1875	  0.01%
 66	    1948	  0.01%
 67	    2183	  0.01%
 68	    2608	  0.02%
 69	    2902	  0.02%
 70	    3207	  0.02%
 71	    3653	  0.02%
 72	    4210	  0.03%
 73	    4684	  0.03%
 74	    5042	  0.03%
 75	    5518	  0.03%
 76	    6304	  0.04%
 77	    6723	  0.04%
 78	    7588	  0.05%
 79	    8146	  0.05%
 80	    9202	  0.06%
 81	   10221	  0.06%
 82	   11242	  0.07%
 83	   12493	  0.08%
 84	   14144	  0.09%
 85	   15023	  0.10%
 86	   16135	  0.10%
 87	   17990	  0.11%
 88	   19406	  0.12%
 89	   20812	  0.13%
 90	   22689	  0.14%
 91	   25148	  0.16%
 92	   27215	  0.17%
 93	   29530	  0.19%
 94	   32076	  0.20%
 95	   33547	  0.21%
 96	   35951	  0.23%
 97	   38543	  0.24%
 98	   40747	  0.26%
 99	   42826	  0.27%
100	   46259	  0.29%
101	15183038	 96.18%


criterion=sequence-density
sequence-density=0.23
sequence-density-rank=1
fanout-score=2.25
fanout-score-rank=36
prefix-density=0.23
prefix-fanout=2.2
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=17
fanout-score=102.72
fanout-score-rank=1
prefix-density=0.35
prefix-fanout=15.7
sequence=AAAACAAAACAAAA


criterion=sequence-density
sequence-density=0.17
sequence-density-rank=1
fanout-score=2.49
fanout-score-rank=37
prefix-density=0.19
prefix-fanout=2.3
sequence=CCTAATCATATT


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=15
fanout-score=105.94
fanout-score-rank=1
prefix-density=0.35
prefix-fanout=18.3
sequence=AAGAAGAAGAAA
SRR32570304 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 03:39:03
                             Started mapping on |	Mar 31 03:39:03
                                    Finished on |	Mar 31 03:47:43
       Mapping speed, Million of reads per hour |	327.90

                          Number of input reads |	47363364
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	36576544
                        Uniquely mapped reads % |	77.23%
                          Average mapped length |	200.34
                       Number of splices: Total |	19454664
            Number of splices: Annotated (sjdb) |	19245482
                       Number of splices: GT/AG |	19238921
                       Number of splices: GC/AG |	164346
                       Number of splices: AT/AC |	18724
               Number of splices: Non-canonical |	32673
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.79
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.39
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2349066
             % of reads mapped to multiple loci |	4.96%
        Number of reads mapped to too many loci |	85421
             % of reads mapped to too many loci |	0.18%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	17.55%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8437754	8437754	8437754
N_multimapping	2349066	2349066	2349066
N_noFeature	3905965	35311249	4116149
N_ambiguous	1553529	28062	511260
UnstrandedReadsAssigned:31117050 PositiveStrandReadsAssigned:1237233 NegativeStrandReadsAssigned:31949135
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32570304 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32570304-trimmed-pair1.fastq
                             SRR32570304-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 47,363,364 reads, 36,341,618 reads pseudoaligned
[quant] estimated average fragment length: 183.4
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,211 rounds

 109283 SRR32570304.ke.tsv
  52636 SRR32570304.se.tsv
 161919 total
==> SRR32570304.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR32570304.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	450
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32570304 completed mapping pipeline successfully
