Starting /dee2/code/volunteer_pipeline.sh SRR32570298
    current disk space = 833608804
    free memory = 199115628 
SRR32570298 SRAfilesize
d7aaf3d1b4eba17dc00e7e610867ffef  SRR32570298.sra
SRR32570298.sra file validated
SRR32570298 is paired end
SRR32570298 is conventional basespace
SRR32570298 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32570298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0295	34.0	34.0	34.0	34.0	34.0
2	33.1865	34.0	34.0	34.0	34.0	34.0
3	32.9025	34.0	34.0	34.0	34.0	34.0
4	33.222	34.0	34.0	34.0	34.0	34.0
5	33.3685	34.0	34.0	34.0	34.0	34.0
6	33.4175	34.0	34.0	34.0	34.0	34.0
7	33.3975	34.0	34.0	34.0	34.0	34.0
8	33.501	34.0	34.0	34.0	34.0	34.0
9	33.5345	34.0	34.0	34.0	34.0	34.0
10-11	33.50575	34.0	34.0	34.0	34.0	34.0
12-13	33.48675	34.0	34.0	34.0	34.0	34.0
14-15	33.54625	34.0	34.0	34.0	34.0	34.0
16-17	33.453500000000005	34.0	34.0	34.0	34.0	34.0
18-19	33.464749999999995	34.0	34.0	34.0	34.0	34.0
20-21	33.522000000000006	34.0	34.0	34.0	34.0	34.0
22-23	33.538250000000005	34.0	34.0	34.0	34.0	34.0
24-25	33.48025	34.0	34.0	34.0	34.0	34.0
26-27	33.5595	34.0	34.0	34.0	34.0	34.0
28-29	33.567	34.0	34.0	34.0	34.0	34.0
30-31	33.538250000000005	34.0	34.0	34.0	34.0	34.0
32-33	33.448	34.0	34.0	34.0	34.0	34.0
34-35	33.588	34.0	34.0	34.0	34.0	34.0
36-37	33.5925	34.0	34.0	34.0	34.0	34.0
38-39	33.5065	34.0	34.0	34.0	34.0	34.0
40-41	33.576750000000004	34.0	34.0	34.0	34.0	34.0
42-43	33.539500000000004	34.0	34.0	34.0	34.0	34.0
44-45	33.55975	34.0	34.0	34.0	34.0	34.0
46-47	33.47925	34.0	34.0	34.0	34.0	34.0
48-49	33.51175	34.0	34.0	34.0	34.0	34.0
50-51	33.4305	34.0	34.0	34.0	34.0	34.0
52-53	33.478	34.0	34.0	34.0	34.0	34.0
54-55	33.456999999999994	34.0	34.0	34.0	34.0	34.0
56-57	33.38075	34.0	34.0	34.0	34.0	34.0
58-59	33.39775	34.0	34.0	34.0	34.0	34.0
60-61	33.4675	34.0	34.0	34.0	34.0	34.0
62-63	33.2465	34.0	34.0	34.0	34.0	34.0
64-65	33.432	34.0	34.0	34.0	34.0	34.0
66-67	33.460750000000004	34.0	34.0	34.0	34.0	34.0
68-69	33.4025	34.0	34.0	34.0	34.0	34.0
70-71	33.491	34.0	34.0	34.0	34.0	34.0
72-73	33.41775	34.0	34.0	34.0	34.0	34.0
74-75	33.39425	34.0	34.0	34.0	34.0	34.0
76-77	33.40675	34.0	34.0	34.0	34.0	34.0
78-79	33.411249999999995	34.0	34.0	34.0	34.0	34.0
80-81	33.458	34.0	34.0	34.0	34.0	34.0
82-83	33.426249999999996	34.0	34.0	34.0	34.0	34.0
84-85	33.3905	34.0	34.0	34.0	34.0	34.0
86-87	33.38225	34.0	34.0	34.0	34.0	34.0
88-89	33.338499999999996	34.0	34.0	34.0	34.0	34.0
90-91	33.38225	34.0	34.0	34.0	34.0	34.0
92-93	33.4215	34.0	34.0	34.0	34.0	34.0
94-95	33.36925	34.0	34.0	34.0	34.0	34.0
96-97	33.3625	34.0	34.0	34.0	34.0	34.0
98-99	33.41475	34.0	34.0	34.0	34.0	34.0
100-101	33.1135	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	3.0
24	2.0
25	14.0
26	11.0
27	13.0
28	25.0
29	42.0
30	58.0
31	114.0
32	231.0
33	2703.0
34	784.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.4	15.55	12.275	28.775000000000002
2	24.15	18.224999999999998	32.125	25.5
3	22.45	27.125	26.35	24.075
4	20.25	32.125	26.775	20.849999999999998
5	21.725	34.875	26.700000000000003	16.7
6	22.825	34.599999999999994	24.8	17.775
7	14.124999999999998	25.3	42.95	17.625
8	21.0	26.025	32.675	20.3
9	17.025000000000002	27.500000000000004	32.5	22.975
10-11	19.7125	35.5	26.2625	18.525
12-13	20.4	28.575	31.125000000000004	19.900000000000002
14-15	19.425	28.262500000000003	30.725	21.587500000000002
16-17	20.7625	29.6375	30.5	19.1
18-19	21.557336004006007	28.329994992488732	29.431647471206812	20.681021532298445
20-21	19.2625	29.875	30.4875	20.375
22-23	20.6125	29.5375	27.85	22.0
24-25	21.075	28.749999999999996	29.062500000000004	21.1125
26-27	20.45	30.162499999999998	27.725	21.6625
28-29	19.625	27.0625	31.15	22.162499999999998
30-31	19.9125	28.962500000000002	30.65	20.474999999999998
32-33	19.811794228356337	28.54454203262233	29.460476787954832	22.1831869510665
34-35	20.95	29.099999999999998	29.875	20.075000000000003
36-37	20.65	27.875	28.962500000000002	22.5125
38-39	21.036165686397197	28.206732574145914	29.921161306469777	20.83594043298711
40-41	20.4375	29.125	29.462500000000002	20.974999999999998
42-43	19.8	29.025000000000002	30.2625	20.9125
44-45	23.0125	28.237499999999997	27.5875	21.1625
46-47	20.3375	28.9125	30.412499999999998	20.3375
48-49	20.150000000000002	28.825	31.337500000000002	19.6875
50-51	20.3125	30.2875	26.887499999999996	22.5125
52-53	19.4625	29.4375	29.049999999999997	22.05
54-55	21.5	28.525	28.625	21.349999999999998
56-57	19.483773963162513	27.84112266633254	30.04636010524997	22.62874326525498
58-59	21.1875	28.825	29.0875	20.9
60-61	19.90740740740741	29.19169169169169	29.366866866866864	21.534034034034033
62-63	21.633165829145728	28.216080402010054	28.32914572864322	21.821608040201003
64-65	17.862958787423274	28.585744707503448	30.97832894901666	22.572967556056618
66-67	20.2375	29.1125	28.125	22.525000000000002
68-69	20.983483483483482	29.09159159159159	26.989489489489486	22.935435435435437
70-71	18.4375	29.6375	28.787499999999998	23.1375
72-73	18.086303939962477	29.756097560975608	30.30644152595372	21.851156973108193
74-75	20.4125	28.5875	29.7125	21.2875
76-77	20.837500000000002	29.812499999999996	28.675	20.674999999999997
78-79	21.375	30.6875	27.175	20.7625
80-81	21.85	28.962500000000002	28.812500000000004	20.375
82-83	21.25	28.749999999999996	29.849999999999998	20.150000000000002
84-85	21.191638502941544	29.703342095381146	29.678307673050448	19.42671172862686
86-87	22.2125	28.775000000000002	29.549999999999997	19.4625
88-89	21.76581089542893	27.664370695053226	30.782717595491548	19.7871008140263
90-91	22.4625	27.950000000000003	28.175	21.4125
92-93	21.825	27.875	29.4375	20.8625
94-95	21.575	28.349999999999998	29.3375	20.7375
96-97	21.959163221846424	29.124389327320554	28.57321808843793	20.34322936239509
98-99	22.175	30.887500000000003	25.1	21.837500000000002
100-101	22.752316553969447	29.163536188329577	28.274480340596043	19.809666917104934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	3.5
17	8.5
18	12.5
19	12.0
20	11.0
21	11.0
22	8.5
23	9.5
24	7.5
25	16.0
26	29.0
27	29.5
28	35.0
29	46.0
30	57.0
31	71.0
32	87.5
33	101.5
34	87.5
35	72.5
36	94.0
37	106.5
38	114.0
39	189.0
40	211.5
41	192.0
42	227.0
43	232.0
44	218.0
45	208.5
46	207.0
47	198.0
48	187.5
49	174.0
50	159.0
51	145.0
52	112.5
53	86.0
54	69.5
55	42.5
56	32.0
57	29.0
58	14.0
59	12.5
60	10.5
61	3.5
62	1.5
63	2.0
64	1.5
65	1.0
66	0.5
67	0.0
68	0.0
69	0.5
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.15
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.375
34-35	0.0
36-37	0.0
38-39	0.11249999999999999
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.2375
58-59	0.0
60-61	0.1
62-63	0.5
64-65	0.21250000000000002
66-67	0.0
68-69	0.1
70-71	0.0
72-73	0.0625
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.13749999999999998
86-87	0.0
88-89	0.1875
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.21250000000000002
98-99	0.0
100-101	0.17500000000000002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.45192829983704	22.3
2	24.00869092884302	22.1
3	12.873438348723521	17.775
4	7.1156979902227055	13.100000000000001
5	3.1504617055947857	7.249999999999999
6	1.4665942422596416	4.05
7	1.0863661053775122	3.5000000000000004
8	0.27159152634437805	1.0
9	0.32590983161325365	1.35
>10	1.249321021184139	7.575
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	28	0.7000000000000001	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	23	0.575	No Hit
GGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAA	20	0.5	No Hit
GAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTA	19	0.475	No Hit
CCTAAATCAATGAGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTG	14	0.35000000000000003	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	14	0.35000000000000003	No Hit
GTGAAGTGTATGGTTTTGTATTTTGAAACCAAACAACAGTAGCTGAGGTA	13	0.325	No Hit
GGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCT	12	0.3	No Hit
CTTCATTTCATCACAAATTGTAACTTACTTAACTGACCAAAAAAACTATG	12	0.3	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	12	0.3	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	11	0.27499999999999997	No Hit
GAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAAT	11	0.27499999999999997	No Hit
GGTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTCA	11	0.27499999999999997	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	11	0.27499999999999997	No Hit
GCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTC	11	0.27499999999999997	No Hit
CTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGC	11	0.27499999999999997	No Hit
GGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTTCTTTGTTGCTATCA	10	0.25	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	10	0.25	No Hit
CCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGC	10	0.25	No Hit
TTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGAGAC	10	0.25	No Hit
GTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAA	10	0.25	No Hit
CCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCA	10	0.25	No Hit
GCACTCTATTTGAGTAGATCAACATCTTTGCCAGCCAGCAGACCAGAAAT	10	0.25	No Hit
GTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTGGTTTGT	9	0.22499999999999998	No Hit
GCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAAC	9	0.22499999999999998	No Hit
GCAATATGAAAAGACATAGCCCAAAGCTCAATCAGCAGCAATTTAATTAA	9	0.22499999999999998	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	9	0.22499999999999998	No Hit
GAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACA	9	0.22499999999999998	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	9	0.22499999999999998	No Hit
GCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACC	8	0.2	No Hit
CTTTGCCAGCCAGCAGACCAGAAATAGTAAGATCAACTGCCCAAGATTAC	8	0.2	No Hit
CCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGA	8	0.2	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	8	0.2	No Hit
TTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTC	8	0.2	No Hit
GCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCA	7	0.17500000000000002	No Hit
CCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGT	7	0.17500000000000002	No Hit
CTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTCCTTCATTAAA	7	0.17500000000000002	No Hit
TGATAAAATTATGAAACATAGCAAAAGGCTAAAAGAAGATTTCTTCCCCA	7	0.17500000000000002	No Hit
AGGTGAAGAAATATCCTCACTCTTTTATATGGGGGAAAAAATGAGGCAAA	7	0.17500000000000002	No Hit
AGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTT	7	0.17500000000000002	No Hit
AAGCCATTATGTGGAGGAGCTGTTCAGCTCTCTCTAACCTATTTAGATTT	7	0.17500000000000002	No Hit
GGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCA	7	0.17500000000000002	No Hit
CTCTATAGAATGTTCTCTCCACCACCTCCTGCTAGTCATACCAAACCCAC	7	0.17500000000000002	No Hit
GTCCATATGAGGGATTAATTGTTCTCATTATAAAGTTTATAATTTCTGCC	7	0.17500000000000002	No Hit
CCCAAAATAATCCTGTTAGAAGGAATGCCATTCTTCACTTCTTGATCTAT	7	0.17500000000000002	No Hit
CAAATCTCATCTTAATAATACCTGTTACTTATTGTCCAGCAGAGGATCAG	7	0.17500000000000002	No Hit
GCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGT	7	0.17500000000000002	No Hit
GTGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	7	0.17500000000000002	No Hit
AGGAAGCCATCTTCATACCTGTCCCTCTGCATTTATTCTTGAGGTTACTG	7	0.17500000000000002	No Hit
AAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAATTG	7	0.17500000000000002	No Hit
TCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTG	7	0.17500000000000002	No Hit
CCACAGTGTACTTTATTTAATGATTTTTGTACTTTGTGTTGCAATAAAAT	7	0.17500000000000002	No Hit
CGTAATATCAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTG	7	0.17500000000000002	No Hit
GGGTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAG	7	0.17500000000000002	No Hit
GCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCT	6	0.15	No Hit
TGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTCTT	6	0.15	No Hit
GTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGTATAGCAAAT	6	0.15	No Hit
CACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAGC	6	0.15	No Hit
GCTTTTCAGACAATGGTGGCATCCGCCCTTCTTCTTCCTGTGATGCACAC	6	0.15	No Hit
CTGTCTTTAAAAATTGTTTTGGGGCTTTGTACCAAAAAGTCCCAGGTTGA	6	0.15	No Hit
CAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCTTC	6	0.15	No Hit
CCACACACTGTGCTTTGAGCTAGAGCACTTGGAGTCACTGCCCAGGGCAG	6	0.15	No Hit
GTGGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAAT	6	0.15	No Hit
AGGATCTGTAGTGATGTCCTAACATGCTTGCTCTCTCATAACCAGACTTT	6	0.15	No Hit
GGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATA	6	0.15	No Hit
GAAATATGTAGAAAAAGAATCATCCCATCAAAGCTAGTTAGTACGAGTGT	6	0.15	No Hit
TCATACTTACATGCTCTGGAATGCGGCCTCTCTGCTTGGGATCAGTGCTT	6	0.15	No Hit
AGGCGCCTCCCTGAGAAAATCCTCCCAAAATAATCCTGTTAGAAGGAATG	6	0.15	No Hit
GGATTCCACGTGGAATAGAAACCACTGAAAAGAGACTCAACTCTTTTTCC	6	0.15	No Hit
CCATGTTCCTGCAATTTAGTGAGAGGCCTGGCGGTCTCTAACCCAAGTGC	6	0.15	No Hit
GCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTG	6	0.15	No Hit
GCGCAAGATATTTAGGGAGTGATCAAGGAATCAAGCACAGATGTTCCAAG	6	0.15	No Hit
CTGCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGCACTTG	6	0.15	No Hit
TTTTTTTTTTAAGTAGAAGCAAGATTCTCATTAAGGCTAACTATGCTATT	6	0.15	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	6	0.15	No Hit
CCACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAG	6	0.15	No Hit
ACCACACGCATATCTGACTGTTGCTTACTCTGAACCTCTATCTGAATTTC	6	0.15	No Hit
CTGACATGGCTGTTTGGGGAGCCAACTCTATTATCTTCTATATGAACTAA	6	0.15	No Hit
CATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGA	6	0.15	No Hit
GGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAG	6	0.15	No Hit
ACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGTAT	6	0.15	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	5	0.125	No Hit
TAAGTGAGCAAAAAGAGGTTTCAGAACCAAGGGTCTGGAACAGCCTCTTC	5	0.125	No Hit
TCATACCTGTCCCTCTGCATTTATTCTTGAGGTTACTGAGCCATCTGGCT	5	0.125	No Hit
TTACGGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCT	5	0.125	No Hit
GGGGACCCAGGTGTGTACAGATTCCTTGCTTTTTTGTTGCTATCATTGAA	5	0.125	No Hit
GGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGGTTGA	5	0.125	No Hit
GTATAGAGATAGAGTAGGGATGGGGACCCAGGTGTGTACAGATTCCTTGC	5	0.125	No Hit
CGCAGAAAGAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTC	5	0.125	No Hit
GGAAGTAGAAGATTTTTCTCTTTGTGTCTGTGTCTTTCATTTGCCTATGA	5	0.125	No Hit
TCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTC	5	0.125	No Hit
CCCCAGCAATATTATGTTGCTCTGTGCTGCTCTATGCATATGGCATGTTG	5	0.125	No Hit
GCCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	5	0.125	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	5	0.125	No Hit
AACCAAGAAAGACACACTTACAAGTTGAATTCTAAAGGAAGGTCTGGTTA	5	0.125	No Hit
GGTAGCCAGGCCTTGCCCTTTCATAAGAAGACATGTGAAGTACCATAATT	5	0.125	No Hit
CTCACATTCAATTCCCTCCTTAGACAATACAGCTGCAGCTTCTAGGCAGT	5	0.125	No Hit
CTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACTATGTC	5	0.125	No Hit
CGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCA	5	0.125	No Hit
GGCTTTCTTCCATCTTTTTAAAATGACTTGTCTAATCTTTAATATATCAG	5	0.125	No Hit
CACTGAGAAAGTAAACTTGCTGCTAGTGGTGGTATTGACCTGTAGCCCTC	5	0.125	No Hit
GGTTTCTGCTGCCTGTTTAATTCCAGATTCATCTTCCTGGGAATCTGGTG	5	0.125	No Hit
GTAGTAACAACACCGCCATTTTTTTCAATGGCAGCAATGGCTAATTCTGA	5	0.125	No Hit
GTACAGTCTCTTTGTGCCAGGAGGATGTGACTTTCTTTTTAAATCCCAAA	5	0.125	No Hit
ATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAATTGTAACT	5	0.125	No Hit
GTACCATTCTCTTCCATTGAGAATTCTAAGCCATTATGTGGAGGAGCTGT	5	0.125	No Hit
GGAAGGTCTGGTTATGTGGGGTTGGAATATGTATATCTATATATCTCTAT	5	0.125	No Hit
CTCCACCCCATCCTTCGCAGACACGAGTCTGACTTTATTATGTCATTTTC	5	0.125	No Hit
CTGAGAAGCTATAGGCTAGTTGATTCCTTATAGAAATGGATATTGGTTTG	5	0.125	No Hit
GCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAGTATCAGCACCCTC	5	0.125	No Hit
GTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTT	5	0.125	No Hit
GGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCG	5	0.125	No Hit
GTTGCCTGCACACACCTTATTTCTGAAATGGAGAGAGAAACTCAAGAATG	5	0.125	No Hit
AGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAA	5	0.125	No Hit
TTTTAAGTAGAAGCAAGATTCTCATTAAGGCTAACTATGCTATTCTCCAC	5	0.125	No Hit
GCAAACCTTCAGCTGGCATCAGGTACATAAATGGTACACAGACGCACATG	5	0.125	No Hit
CTCTTTGGTTGGTGATTTAGTCCCTGGGAGCTCTGGGGGGTTTCTGGTTG	5	0.125	No Hit
GGAAGATCTAGAAATTGAAATGATCTGAGGTACTAAAAGTTTGGGGAGAG	5	0.125	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	5	0.125	No Hit
GCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTA	5	0.125	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	5	0.125	No Hit
CTTATTATAAAGTTTATAATTTCTGCCTAGACTTTTAATGGAAAGTTTTC	5	0.125	No Hit
CTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTC	5	0.125	No Hit
GTTGCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCA	5	0.125	No Hit
CCAGCTATTACTCCTCTTTTGCTCAGAGATAAATTTAAAATACAAACATT	5	0.125	No Hit
GCCGTATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	5	0.125	No Hit
CGGATCCTTCCGAGCGCTGAGCATTCTGAAGAGTTCATCTTTCGTAATAT	5	0.125	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	5	0.125	No Hit
GTTCTTTTGGATGCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTT	5	0.125	No Hit
CGAGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAA	5	0.125	No Hit
GCTTTTGCTGTAACTTCAGTTGTTGGCTGGAAATTTCTTGAAGACAAAGA	5	0.125	No Hit
GTGTATTGAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCA	5	0.125	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	5	0.125	No Hit
GTACTGCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGCAC	5	0.125	No Hit
TTTGTCATTTTGTTGAAGGTTGCATTTCTCAGTTGGGAGGACACCAGCAC	5	0.125	No Hit
GTCTTCATCTACCAGCTATTACTCCTCTTTTGCTCAGAGATAAATTTAAA	5	0.125	No Hit
GCCTGAAACTATGTCCTTCATTAAATCCATATTTTGGGATTCGTATGTAA	5	0.125	No Hit
CCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTC	5	0.125	No Hit
AGATACTGTAGTCTCCTGAGACTCAAAGGCTGATACTGGTGCCTGAAACT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0125	0.0	0.0	0.0	0.0
74-75	0.037500000000000006	0.0	0.0	0.0	0.0
76-77	0.05	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.15	0.0	0.0	0.0	0.0
82-83	0.21250000000000002	0.0	0.0	0.0	0.0
84-85	0.2375	0.0	0.0	0.0	0.0
86-87	0.3625	0.0	0.0	0.0	0.0
88-89	0.4375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGTAT	25	0.004770157	56.6775	3
>>END_MODULE
SRR32570298 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32570298_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.2525	34.0	34.0	34.0	34.0	34.0
2	33.3545	34.0	34.0	34.0	34.0	34.0
3	33.149	34.0	34.0	34.0	34.0	34.0
4	33.1735	34.0	34.0	34.0	34.0	34.0
5	33.22	34.0	34.0	34.0	34.0	34.0
6	33.2665	34.0	34.0	34.0	34.0	34.0
7	33.3995	34.0	34.0	34.0	34.0	34.0
8	33.3265	34.0	34.0	34.0	34.0	34.0
9	33.384	34.0	34.0	34.0	34.0	34.0
10-11	33.312749999999994	34.0	34.0	34.0	34.0	34.0
12-13	33.323	34.0	34.0	34.0	34.0	34.0
14-15	33.31925	34.0	34.0	34.0	34.0	34.0
16-17	33.28075	34.0	34.0	34.0	34.0	34.0
18-19	33.332750000000004	34.0	34.0	34.0	34.0	34.0
20-21	33.321749999999994	34.0	34.0	34.0	34.0	34.0
22-23	33.24375	34.0	34.0	34.0	34.0	34.0
24-25	33.25375	34.0	34.0	34.0	34.0	34.0
26-27	33.407250000000005	34.0	34.0	34.0	34.0	34.0
28-29	33.28275	34.0	34.0	34.0	34.0	34.0
30-31	33.35575	34.0	34.0	34.0	34.0	34.0
32-33	33.351	34.0	34.0	34.0	34.0	34.0
34-35	33.29925	34.0	34.0	34.0	34.0	34.0
36-37	33.319500000000005	34.0	34.0	34.0	34.0	34.0
38-39	33.34025	34.0	34.0	34.0	34.0	34.0
40-41	33.35675	34.0	34.0	34.0	34.0	34.0
42-43	33.267250000000004	34.0	34.0	34.0	34.0	34.0
44-45	33.33175	34.0	34.0	34.0	34.0	34.0
46-47	33.24825	34.0	34.0	34.0	34.0	34.0
48-49	33.15175	34.0	34.0	34.0	34.0	34.0
50-51	33.2675	34.0	34.0	34.0	34.0	34.0
52-53	33.24825	34.0	34.0	34.0	34.0	34.0
54-55	33.333	34.0	34.0	34.0	34.0	34.0
56-57	33.29925	34.0	34.0	34.0	34.0	34.0
58-59	33.3375	34.0	34.0	34.0	34.0	34.0
60-61	33.345	34.0	34.0	34.0	34.0	34.0
62-63	33.27275	34.0	34.0	34.0	34.0	34.0
64-65	33.297	34.0	34.0	34.0	34.0	34.0
66-67	33.3305	34.0	34.0	34.0	34.0	34.0
68-69	33.33975	34.0	34.0	34.0	34.0	34.0
70-71	33.24325	34.0	34.0	34.0	34.0	34.0
72-73	33.28825	34.0	34.0	34.0	34.0	34.0
74-75	33.365	34.0	34.0	34.0	34.0	34.0
76-77	33.23075	34.0	34.0	34.0	34.0	34.0
78-79	33.286500000000004	34.0	34.0	34.0	34.0	34.0
80-81	33.246750000000006	34.0	34.0	34.0	34.0	34.0
82-83	33.22825	34.0	34.0	34.0	34.0	34.0
84-85	33.165000000000006	34.0	34.0	34.0	34.0	34.0
86-87	33.2175	34.0	34.0	34.0	34.0	34.0
88-89	33.25075	34.0	34.0	34.0	34.0	34.0
90-91	33.241749999999996	34.0	34.0	34.0	34.0	34.0
92-93	33.13275	34.0	34.0	34.0	34.0	34.0
94-95	33.207750000000004	34.0	34.0	34.0	34.0	34.0
96-97	33.14475	34.0	34.0	34.0	34.0	34.0
98-99	33.1405	34.0	34.0	34.0	34.0	34.0
100-101	33.004999999999995	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	4.0
22	4.0
23	10.0
24	20.0
25	16.0
26	17.0
27	22.0
28	31.0
29	39.0
30	55.0
31	95.0
32	266.0
33	2875.0
34	544.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.825	26.075	14.85	22.25
2	24.125	28.675	30.599999999999998	16.6
3	20.575	31.075000000000003	31.525	16.825000000000003
4	23.275000000000002	35.8	24.5	16.425
5	20.5	35.15	24.875	19.475
6	21.175	34.55	25.275	19.0
7	17.45	23.1	37.525	21.925
8	20.150000000000002	26.650000000000002	26.650000000000002	26.55
9	19.400000000000002	28.025	30.075000000000003	22.5
10-11	23.0125	33.125	23.5875	20.275000000000002
12-13	19.6	27.3375	30.012499999999996	23.05
14-15	21.712500000000002	28.8625	27.8625	21.5625
16-17	20.588235294117645	29.574468085106382	27.271589486858574	22.5657071339174
18-19	21.512500000000003	28.1875	28.962500000000002	21.337500000000002
20-21	20.474999999999998	30.625000000000004	28.549999999999997	20.349999999999998
22-23	20.6875	30.275000000000002	27.187499999999996	21.85
24-25	21.275	29.512500000000003	29.1875	20.025000000000002
26-27	21.4875	29.262500000000003	27.950000000000003	21.3
28-29	21.727158948685858	29.93742177722153	26.533166458072593	21.802252816020026
30-31	21.2375	29.762499999999996	28.15	20.849999999999998
32-33	21.6625	30.0375	27.950000000000003	20.349999999999998
34-35	21.8875	29.525000000000002	27.6375	20.95
36-37	20.599999999999998	30.175	29.1875	20.0375
38-39	22.725	30.0	27.075	20.200000000000003
40-41	21.2375	27.625	27.775	23.3625
42-43	20.93808630393996	29.956222639149466	26.879299562226393	22.22639149468418
44-45	22.125	29.2	28.1625	20.5125
46-47	21.5625	30.837500000000002	26.35	21.25
48-49	21.905956112852664	28.601880877742946	28.927899686520377	20.564263322884013
50-51	20.200000000000003	31.637500000000003	27.6625	20.5
52-53	20.8125	31.4875	27.9125	19.787499999999998
54-55	22.162499999999998	30.875000000000004	26.7625	20.200000000000003
56-57	20.275000000000002	29.125	27.6875	22.912499999999998
58-59	21.1375	29.4875	28.125	21.25
60-61	21.825	26.9625	30.062499999999996	21.15
62-63	21.0375	28.037499999999998	28.9125	22.0125
64-65	23.95	29.099999999999998	26.325	20.625
66-67	22.4875	28.9875	27.8875	20.6375
68-69	20.2375	29.9625	26.875	22.925
70-71	22.625	28.475	27.0125	21.8875
72-73	21.825	29.25	28.787499999999998	20.1375
74-75	22.412499999999998	28.1	28.349999999999998	21.1375
76-77	21.8377836279303	28.444277297229537	27.466466090008772	22.25147298483139
78-79	20.974999999999998	28.3125	27.6	23.1125
80-81	21.76360225140713	28.880550343964977	27.517198248905565	21.838649155722326
82-83	21.196196196196198	28.253253253253252	29.241741741741738	21.30880880880881
84-85	22.3625	28.9375	28.1625	20.5375
86-87	21.8875	29.875	27.3875	20.849999999999998
88-89	20.35785785785786	29.116616616616614	27.89039039039039	22.635135135135133
90-91	21.455410821643287	29.521543086172347	28.920340681362728	20.102705410821645
92-93	20.0225535647162	30.28442551058765	27.089337175792505	22.60368374890365
94-95	22.75	30.312499999999996	27.0	19.9375
96-97	21.712500000000002	29.099999999999998	28.037499999999998	21.15
98-99	22.3625	28.6875	27.962500000000002	20.9875
100-101	21.55	30.3	28.3625	19.787499999999998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.0
18	8.5
19	6.5
20	6.0
21	5.5
22	6.0
23	6.5
24	6.5
25	12.0
26	18.0
27	26.0
28	31.0
29	47.0
30	59.5
31	64.0
32	79.5
33	74.5
34	64.0
35	75.5
36	101.0
37	101.5
38	103.5
39	181.0
40	209.5
41	194.0
42	228.0
43	239.5
44	239.0
45	215.0
46	181.0
47	192.5
48	215.0
49	201.0
50	146.5
51	130.0
52	131.5
53	126.0
54	93.5
55	43.5
56	35.5
57	24.0
58	16.0
59	17.0
60	15.0
61	8.0
62	2.5
63	2.0
64	1.5
65	2.0
66	1.5
67	1.0
68	0.0
69	0.0
70	0.0
71	0.5
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.125
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.125
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0625
44-45	0.0
46-47	0.0
48-49	0.3125
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.2875
78-79	0.0
80-81	0.0625
82-83	0.1
84-85	0.0
86-87	0.0
88-89	0.1
90-91	0.2
92-93	0.2375
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.554603854389725	22.675
2	23.66167023554604	22.1
3	13.650963597430406	19.125
4	6.263383297644539	11.700000000000001
5	3.051391862955032	7.124999999999999
6	1.6059957173447537	4.5
7	1.284796573875803	4.2
8	0.8565310492505354	3.2
9	0.4817987152034261	2.025
>10	0.588865096359743	3.35
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTG	20	0.5	No Hit
GGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTA	14	0.35000000000000003	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	13	0.325	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	12	0.3	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	12	0.3	No Hit
CCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACAC	11	0.27499999999999997	No Hit
TGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAACGTGAAG	11	0.27499999999999997	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	11	0.27499999999999997	No Hit
ATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCT	10	0.25	No Hit
AGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAG	10	0.25	No Hit
AGAGATTCTATCACAAAGTGAAAGATAAAATCTAAATAGGTTAGAGAGAG	10	0.25	No Hit
AAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTT	9	0.22499999999999998	No Hit
TGTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTGACTCC	9	0.22499999999999998	No Hit
GCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGG	9	0.22499999999999998	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	9	0.22499999999999998	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	9	0.22499999999999998	No Hit
CATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAAGAGGGTG	9	0.22499999999999998	No Hit
CATCTGTCCACATGCCCCTGTGATGCCAGTCACATTAAATATGAATATGG	9	0.22499999999999998	No Hit
GTTTTATACAGAAAAACTGAAGTTGACTATTTGGTCTAGTTTTGGGTGGG	9	0.22499999999999998	No Hit
TGTTTTAAACGTGTTTAAAGATATTCTATAAAACAATATTCCCTTCAGTA	9	0.22499999999999998	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	8	0.2	No Hit
GAGAATCTTGCTTCTACTTAAAAAAATAAAAATAAAATAAAAATAAAAAA	8	0.2	No Hit
ATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAA	8	0.2	No Hit
CCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAA	8	0.2	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	8	0.2	No Hit
GAGAGGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGG	8	0.2	No Hit
GTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATTTGCTAT	8	0.2	No Hit
CTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTC	8	0.2	No Hit
AAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACT	8	0.2	No Hit
ATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGA	8	0.2	No Hit
CATGAATATTGTAATGTTGGTTATTTTCCCAGTTAATCTTTAACACATTT	8	0.2	No Hit
AGGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTAC	8	0.2	No Hit
GAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCGATGCAA	8	0.2	No Hit
GTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAA	8	0.2	No Hit
GGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTG	8	0.2	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	8	0.2	No Hit
GTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGC	7	0.17500000000000002	No Hit
GTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGG	7	0.17500000000000002	No Hit
ATTCTATAAAACAATATTCCCTTCAGTATGGAATATATTCCATCCAGGTA	7	0.17500000000000002	No Hit
GTCCAAGAGATCGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGA	7	0.17500000000000002	No Hit
CAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACA	7	0.17500000000000002	No Hit
CTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCC	7	0.17500000000000002	No Hit
GGACCATTTTCTACTTAAATACACTGAACTTTACTAAATGTTTATAAATA	7	0.17500000000000002	No Hit
CATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA	7	0.17500000000000002	No Hit
CTGTAAGCTCTATGCGCTGTTTGAACATGATGCTATAGTCATTCTTTTTG	7	0.17500000000000002	No Hit
AGTGTTTCCATAGATGGACCATTTTCTACTTAAATACACTGAACTTTACT	7	0.17500000000000002	No Hit
CAACTTGTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTG	7	0.17500000000000002	No Hit
GGACATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACA	7	0.17500000000000002	No Hit
CCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCT	7	0.17500000000000002	No Hit
TGTATTTTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAGATG	7	0.17500000000000002	No Hit
TAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAA	7	0.17500000000000002	No Hit
CAAAGCCTCATCACTAAGCTGTGTTTGTCTACCTGGTACGTTAACTATTT	7	0.17500000000000002	No Hit
GGAGGATGTTTGGCTGTGCTTCAGATGTTCTATCCATGAATGCCTGGGGA	7	0.17500000000000002	No Hit
ATGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGAT	7	0.17500000000000002	No Hit
GGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTC	7	0.17500000000000002	No Hit
CTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCCTATT	7	0.17500000000000002	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	7	0.17500000000000002	No Hit
GCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCAT	7	0.17500000000000002	No Hit
GAGATACAGGGCATGGATGGGCAGAAGCCTTTGCAGGTATCAAAAGTCCC	7	0.17500000000000002	No Hit
GGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCAAGTATT	7	0.17500000000000002	No Hit
GCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGC	6	0.15	No Hit
ATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTT	6	0.15	No Hit
GTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAA	6	0.15	No Hit
CTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGAT	6	0.15	No Hit
GAGAAAAATCTTCTACTTCCTAAAAGAACTATTTCCCAGCTTATTTATGT	6	0.15	No Hit
CTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAACGTGA	6	0.15	No Hit
GTACAAATGTATTTGGAATTTTATTTGAAACAAAAATTTAAATGAGTTAT	6	0.15	No Hit
AACCAGACCTTCCTTTAGAATTCAACTTGTAAGTGTGTCTTTCTTGGTTG	6	0.15	No Hit
ATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAG	6	0.15	No Hit
ATTTATGTCAACCATATTACTGATATATTACCTTCACAAAACCGTTTTCA	6	0.15	No Hit
GGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCG	6	0.15	No Hit
ATAGTGACAAGAGGGTATGTGGTTCAATGATAGCAACAAAGAAGCAAGGA	6	0.15	No Hit
CAAAGGTCCAACTAGGTCATAAAAGAGAGAAAAGAGGTTTACTACTTATA	6	0.15	No Hit
CATTTCTATAAGGAATCAACTAGCCTATAGCTTCTCAGATCACATAAAAT	6	0.15	No Hit
GCTGGGCTTTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAAT	6	0.15	No Hit
CTGAACAGCTCCTCCACATAATGGCTTAGAATTCTCAATGGAAGAGAATG	6	0.15	No Hit
TTTAAAATAGTTAAGATCTAGAGCCACCACCTGAAATGGACATGATATCC	6	0.15	No Hit
CCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA	6	0.15	No Hit
ATTTGCTTAATTTGCCTTAAATATTTTGTGCTTTTCTCCTTCCTCTTTCC	6	0.15	No Hit
CCTGCTTCCAGATGGCAAGAGTCTGGGCTCCTTACATTGGTTCTCCATTC	6	0.15	No Hit
CAGCAGGTGTAGCTGCTCAGCACTCGCAATGCTTTGCTGCATGGTATGGG	6	0.15	No Hit
CAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACG	6	0.15	No Hit
GATGCAGTGTGGACTTTGGGTCCCAGCTTGTGCTTTGCTCTTCCTGGGAT	6	0.15	No Hit
GAAGAATATAATAAAAGTCAAAAAAGGGCAGACATGGAATAAAGGTAATT	6	0.15	No Hit
TTTTTAAAGCATGGTTTGGGATTTAAAAAGAAAGTCACATCCTCCTGGCA	6	0.15	No Hit
AAAATATGGATTTAATGAAGGACATAGTTTCAGGCACCAGTATCAGCCTT	6	0.15	No Hit
GAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAA	6	0.15	No Hit
CTTTGATGTAAATATATATAGAGATATATAGATATACATATTCCAACCCC	6	0.15	No Hit
CATTCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAAC	6	0.15	No Hit
GTTTGGTATGACTAGCAGGAGGTGGTGGAGAGAACATTCTATAGAGAGAT	6	0.15	No Hit
GAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATAT	5	0.125	No Hit
TATACATATTCCAACCCCACATAACCAGACCTTCCTTTAGAATTCAACTT	5	0.125	No Hit
GCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATTTGC	5	0.125	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	5	0.125	No Hit
CATAACCAGACCTTCCTTTAGAATTCAACTTGTAAGTGTGTCTTTCTTGG	5	0.125	No Hit
TCAACTTGTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGT	5	0.125	No Hit
GTATCAAATAAAAGAGCTGTTAGAGAAATGACAGAAGGAGCTGAAGGTGT	5	0.125	No Hit
GACTGAACACGCTGTACGGTAAAAGTGGGGCCTCATGAAGCGAAAAGTAA	5	0.125	No Hit
TTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCAT	5	0.125	No Hit
GTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGGCCAAGGC	5	0.125	No Hit
TGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTG	5	0.125	No Hit
ACATATTCCAACCCCACATAACCAGACCTTCCTTTAGAATTCAACTTGTA	5	0.125	No Hit
TGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATT	5	0.125	No Hit
AGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGG	5	0.125	No Hit
GCGGTGTTGTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTG	5	0.125	No Hit
GCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTG	5	0.125	No Hit
GGGGATGTGTGAAGCCAGGGTGAACCCTTTTGTCTAGTCACAGATCTGTG	5	0.125	No Hit
GAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGATGA	5	0.125	No Hit
CAATATAATCTCCTCTGATTCTCTAAAATTAAGTTGTCAATGGACTCAAT	5	0.125	No Hit
AGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCA	5	0.125	No Hit
TTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCT	5	0.125	No Hit
AAGCAACCCAACTCACACCAGACTTGCTTTATTTGAATTTTTTAAAGCAT	5	0.125	No Hit
TTTTTTTACAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTG	5	0.125	No Hit
ATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGT	5	0.125	No Hit
ACTGATGTATGGAGTTGCATTTGAACTTCCTGCAGAAGCTCAGTCAAAAG	5	0.125	No Hit
GTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCA	5	0.125	No Hit
GAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATT	5	0.125	No Hit
GGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCG	5	0.125	No Hit
GGCCAGGAGCTCAAGTGCAGCCTGTGGCCCTGTTCCCTCTCTTAGAAGCA	5	0.125	No Hit
TTTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAGATGAAGAC	5	0.125	No Hit
AATAATGATAAATAATGTCATTTTTTATAATGGCTTCAATACATACACCT	5	0.125	No Hit
GGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATTAA	5	0.125	No Hit
GTGACATTCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGG	5	0.125	No Hit
TTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCC	5	0.125	No Hit
AATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCG	5	0.125	No Hit
GATCATAGGCATACAGGTTGCCTCACCTCCACCAAGTACAGCATGATACC	5	0.125	No Hit
GCATAGAGCAGCACAGAGCAACATAATATTGCTGGGGGGAAAAAGAGTTG	5	0.125	No Hit
GTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAA	5	0.125	No Hit
CAGCTCAGCGTTTTTCCACTTTAAACCAGTTTTCTTATTGAAAAGAAATG	5	0.125	No Hit
AGTATTCTGATGCATAGTCGGTGACATTCTTGAGTTTCTCTCTCCATTTC	5	0.125	No Hit
GTTTTTCTGGGTATGAAACCATCATACTTGGTGAGCCTGCTTCCAGATGG	5	0.125	No Hit
GGCATCCAAAAGAACAAATGTTTTTCTGGGTATGAAACCATCATACTTGG	5	0.125	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	5	0.125	No Hit
ACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGGTGTGACCAT	5	0.125	No Hit
ATGGAGTCCAGCTGGTTGAAGAGGGTGCTGATACTTTTCAAGCAAAAATT	5	0.125	No Hit
ATGTACAATAAGCAGTGTTTTCTAATGAACAAACATTGCCATGCAGTTCA	5	0.125	No Hit
TACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAGTTTCC	5	0.125	No Hit
GTGAAGACTGGCCAATGTCTCCCAGGAAATCAAATATGTGATTTCTTTCA	5	0.125	No Hit
ATATGTGATTATTTGTAAATACAACTGCCAATTTTATGGTTACAGCCAAC	5	0.125	No Hit
GTGCAGGCAACGTGAAGATATATCACCCGGACATCACGGTGCCTGGCCTC	5	0.125	No Hit
GTGCACACTTTATGGCTCCTCTTTGCCATGTCGGAGCTGTCCGCATTGCT	5	0.125	No Hit
AAAAAAAATGAAGAAGAACCTCATGGCTGAAGGTTGCCAAGTTAGAAATT	5	0.125	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	5	0.125	No Hit
CAAAAGAACAAATGTTTTTCTGGGTATGAAACCATCATACTTGGTGAGCC	5	0.125	No Hit
CATTGATTTAGGTCGAGTTGATCCAACTCAACCTATTGACCTAACCCAGC	5	0.125	No Hit
AGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCAC	5	0.125	No Hit
GTAGAGTCCCCAATTCAGCCCTGGTGAGGCCAACTGATCCTCTGCTGGAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0125	0.0	0.0	0.0	0.0
74-75	0.037500000000000006	0.0	0.0	0.0	0.0
76-77	0.05	0.0	0.0	0.0	0.0
78-79	0.125	0.0	0.0	0.0	0.0
80-81	0.15	0.0	0.0	0.0	0.0
82-83	0.21250000000000002	0.0	0.0	0.0	0.0
84-85	0.2375	0.0	0.0	0.0	0.0
86-87	0.3625	0.0	0.0	0.0	0.0
88-89	0.42500000000000004	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 5422060 spots for SRR32570298.sra
Written 5422060 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
Read 5422053 spots for SRR32570298.sra
Written 5422053 spots for SRR32570298.sra
SRR ids: ['SRR32570298.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_d5_h1dgy
SRR32570298.sra spots: 43376431
blocks: [[1, 5422053], [5422054, 10844106], [10844107, 16266159], [16266160, 21688212], [21688213, 27110265], [27110266, 32532318], [32532319, 37954371], [37954372, 43376431]]
SRR32570298 file size 10483536
SRR32570298 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32570298 SRR32570298_1.fastq SRR32570298_2.fastq
Input file:	SRR32570298_1.fastq
Paired file:	SRR32570298_2.fastq
trimmed:	SRR32570298-trimmed-pair1.fastq, SRR32570298-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 04:23:36 2026 >> started

Tue Mar 31 04:24:30 2026 >> done (53.168s)
43376431 read pairs processed; of these:
      66 ( 0.00%) short read pairs filtered out after trimming by size control
   56749 ( 0.13%) empty read pairs filtered out after trimming by size control
43319616 (99.87%) read pairs available; of these:
 1489905 ( 3.44%) trimmed read pairs available after processing
41829711 (96.56%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       8	  0.00%
 19	       5	  0.00%
 20	       8	  0.00%
 21	       8	  0.00%
 22	       5	  0.00%
 23	      13	  0.00%
 24	       6	  0.00%
 25	      10	  0.00%
 26	      15	  0.00%
 27	      16	  0.00%
 28	      21	  0.00%
 29	      38	  0.00%
 30	      48	  0.00%
 31	      49	  0.00%
 32	      49	  0.00%
 33	      69	  0.00%
 34	      64	  0.00%
 35	      87	  0.00%
 36	      88	  0.00%
 37	     126	  0.00%
 38	     134	  0.00%
 39	     199	  0.00%
 40	     193	  0.00%
 41	     249	  0.00%
 42	     271	  0.00%
 43	     285	  0.00%
 44	     305	  0.00%
 45	     306	  0.00%
 46	     392	  0.00%
 47	     482	  0.00%
 48	     583	  0.00%
 49	     618	  0.00%
 50	     712	  0.00%
 51	     897	  0.00%
 52	     917	  0.00%
 53	     921	  0.00%
 54	    1043	  0.00%
 55	    1124	  0.00%
 56	    1302	  0.00%
 57	    1460	  0.00%
 58	    1770	  0.00%
 59	    2051	  0.00%
 60	    2388	  0.01%
 61	    2816	  0.01%
 62	    2989	  0.01%
 63	    3282	  0.01%
 64	    3532	  0.01%
 65	    3899	  0.01%
 66	    4176	  0.01%
 67	    4682	  0.01%
 68	    5219	  0.01%
 69	    6379	  0.01%
 70	    7231	  0.02%
 71	    8404	  0.02%
 72	    9516	  0.02%
 73	   10740	  0.02%
 74	   11801	  0.03%
 75	   12877	  0.03%
 76	   13835	  0.03%
 77	   14920	  0.03%
 78	   16664	  0.04%
 79	   18864	  0.04%
 80	   21225	  0.05%
 81	   24095	  0.06%
 82	   28518	  0.07%
 83	   31320	  0.07%
 84	   34433	  0.08%
 85	   37453	  0.09%
 86	   39366	  0.09%
 87	   42316	  0.10%
 88	   45902	  0.11%
 89	   50093	  0.12%
 90	   55355	  0.13%
 91	   63111	  0.15%
 92	   70597	  0.16%
 93	   76841	  0.18%
 94	   83986	  0.19%
 95	   88848	  0.21%
 96	   91523	  0.21%
 97	   96427	  0.22%
 98	  101682	  0.23%
 99	  107888	  0.25%
100	  117765	  0.27%
101	41829711	 96.56%
43319616 reads passed initial QC


criterion=sequence-density
sequence-density=1.73
sequence-density-rank=1
fanout-score=1.76
fanout-score-rank=34
prefix-density=3.04
prefix-fanout=1.0
sequence=TGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=30
fanout-score=185.45
fanout-score-rank=1
prefix-density=5.67
prefix-fanout=1.0
sequence=CAGGAAATGTTTAGGGAAGAATG


criterion=sequence-density
sequence-density=2.31
sequence-density-rank=1
fanout-score=3.78
fanout-score-rank=19
prefix-density=4.34
prefix-fanout=2.0
sequence=AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=30.18
fanout-score-rank=1
prefix-density=0.04
prefix-fanout=4.9
sequence=AAAAAAAATGCCAATCATGAAAGTCCACTTCAACTTTAGCAAAAAGGGGGGAAAATTCAACAAAAAGTATACTCTGTATGCTGGGATCCCAAGGTTTCAACACACCATCTGTGGGGGGTTCTTTCTTCTGATAATTCTAGAGCCTGTTACCATAGAAAGGTATTTCTTCAATGGCTGGTTGTAGTTAGTTCAT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTAGTGATTTTGGTGAAGGTGCCAGTGGGAATGTTTGTGATGAGACTTTTAGTTGAAATAAGATAAATAGGGTAATTATTGATGAGATAATTGTGATAAATCATGTTGATGT -y AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA -o SRR32570298 SRR32570298_1.fastq SRR32570298_2.fastq
Input file:	SRR32570298_1.fastq
Paired file:	SRR32570298_2.fastq
trimmed:	SRR32570298-trimmed-pair1.fastq, SRR32570298-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGATCCAATAAATATGATTAGGGAAACAATTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGG
-- paired 3' end adapter sequence (-y):	AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 04:26:32 2026 >> started

Tue Mar 31 04:27:09 2026 >> done (37.035s)
14439872 read pairs processed; of these:
      30 ( 0.00%) short read pairs filtered out after trimming by size control
     207 ( 0.00%) empty read pairs filtered out after trimming by size control
14439635 (100.00%) read pairs available; of these:
      75 ( 0.00%) trimmed read pairs available after processing
14439560 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       1	  0.00%
 19	       3	  0.00%
 20	       2	  0.00%
 21	       4	  0.00%
 22	       1	  0.00%
 23	       5	  0.00%
 24	       1	  0.00%
 25	       3	  0.00%
 26	       3	  0.00%
 27	       6	  0.00%
 28	       5	  0.00%
 29	      16	  0.00%
 30	      13	  0.00%
 31	      16	  0.00%
 32	      16	  0.00%
 33	      32	  0.00%
 34	      21	  0.00%
 35	      37	  0.00%
 36	      29	  0.00%
 37	      41	  0.00%
 38	      42	  0.00%
 39	      55	  0.00%
 40	      58	  0.00%
 41	      86	  0.00%
 42	      94	  0.00%
 43	      90	  0.00%
 44	     101	  0.00%
 45	     101	  0.00%
 46	     142	  0.00%
 47	     183	  0.00%
 48	     191	  0.00%
 49	     224	  0.00%
 50	     229	  0.00%
 51	     283	  0.00%
 52	     310	  0.00%
 53	     301	  0.00%
 54	     359	  0.00%
 55	     385	  0.00%
 56	     412	  0.00%
 57	     513	  0.00%
 58	     597	  0.00%
 59	     697	  0.00%
 60	     795	  0.01%
 61	     917	  0.01%
 62	     978	  0.01%
 63	    1076	  0.01%
 64	    1155	  0.01%
 65	    1334	  0.01%
 66	    1394	  0.01%
 67	    1528	  0.01%
 68	    1735	  0.01%
 69	    2139	  0.01%
 70	    2496	  0.02%
 71	    2788	  0.02%
 72	    3126	  0.02%
 73	    3577	  0.02%
 74	    3795	  0.03%
 75	    4213	  0.03%
 76	    4612	  0.03%
 77	    5026	  0.03%
 78	    5491	  0.04%
 79	    6249	  0.04%
 80	    7042	  0.05%
 81	    8029	  0.06%
 82	    9514	  0.07%
 83	   10454	  0.07%
 84	   11484	  0.08%
 85	   12617	  0.09%
 86	   13097	  0.09%
 87	   14371	  0.10%
 88	   15250	  0.11%
 89	   16613	  0.12%
 90	   18225	  0.13%
 91	   20994	  0.15%
 92	   23609	  0.16%
 93	   25438	  0.18%
 94	   27702	  0.19%
 95	   29619	  0.21%
 96	   30438	  0.21%
 97	   32440	  0.22%
 98	   34059	  0.24%
 99	   35878	  0.25%
100	   39192	  0.27%
101	13943438	 96.56%


criterion=sequence-density
sequence-density=0.25
sequence-density-rank=1
fanout-score=2.19
fanout-score-rank=34
prefix-density=0.27
prefix-fanout=2.0
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=114.03
fanout-score-rank=1
prefix-density=0.19
prefix-fanout=9.0
sequence=TTTTTTTAAATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTC


criterion=sequence-density
sequence-density=0.22
sequence-density-rank=1
fanout-score=2.61
fanout-score-rank=39
prefix-density=0.24
prefix-fanout=2.4
sequence=CCTAATCATATT


criterion=fanout-score
sequence-density=0.06
sequence-density-rank=12
fanout-score=59.93
fanout-score-rank=1
prefix-density=0.33
prefix-fanout=11.4
sequence=GAAGAAGAAGAAAAGGCTCCGGGTTGACAAATGGTGGGGTAACAGAAAGGAACTGGCCACCGTCAGGACCATCTGC
SRR32570298 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 04:29:38
                             Started mapping on |	Mar 31 04:29:38
                                    Finished on |	Mar 31 04:36:51
       Mapping speed, Million of reads per hour |	360.16

                          Number of input reads |	43319379
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	34479945
                        Uniquely mapped reads % |	79.59%
                          Average mapped length |	200.58
                       Number of splices: Total |	16979883
            Number of splices: Annotated (sjdb) |	16784092
                       Number of splices: GT/AG |	16775436
                       Number of splices: GC/AG |	145825
                       Number of splices: AT/AC |	17040
               Number of splices: Non-canonical |	41582
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.81
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.36
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2687786
             % of reads mapped to multiple loci |	6.20%
        Number of reads mapped to too many loci |	72350
             % of reads mapped to too many loci |	0.17%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	13.94%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6151648	6151648	6151648
N_multimapping	2687786	2687786	2687786
N_noFeature	3144912	33248452	3343212
N_ambiguous	1476118	32758	453193
UnstrandedReadsAssigned:29858915 PositiveStrandReadsAssigned:1198735 NegativeStrandReadsAssigned:30683540
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32570298 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32570298-trimmed-pair1.fastq
                             SRR32570298-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 43,319,379 reads, 34,665,215 reads pseudoaligned
[quant] estimated average fragment length: 181.83
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,329 rounds

 109283 SRR32570298.ke.tsv
  52636 SRR32570298.se.tsv
 161919 total
==> SRR32570298.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8.5	0	0
ENSMUST00000196221.1	9	6	0	0
ENSMUST00000179664.1	11	8	0	0
ENSMUST00000178537.1	12	9	0	0
ENSMUST00000178862.1	14	6.5	0	0
ENSMUST00000179520.1	11	8	0	0
ENSMUST00000179883.1	16	8.5	0	0
ENSMUST00000195858.1	10	7	0	0
ENSMUST00000179932.1	12	9	0	0

==> SRR32570298.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	234
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32570298 completed mapping pipeline successfully
