Starting /dee2/code/volunteer_pipeline.sh SRR32570290
    current disk space = 837814560
    free memory = 201939324 
SRR32570290 SRAfilesize
cd422e39815ebcf1e48a266be27dde59  SRR32570290.sra
SRR32570290.sra file validated
SRR32570290 is paired end
SRR32570290 is conventional basespace
SRR32570290 read1 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32570290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.956	34.0	34.0	34.0	34.0	34.0
2	33.229	34.0	34.0	34.0	34.0	34.0
3	33.103	34.0	34.0	34.0	34.0	34.0
4	33.3265	34.0	34.0	34.0	34.0	34.0
5	33.4355	34.0	34.0	34.0	34.0	34.0
6	33.4065	34.0	34.0	34.0	34.0	34.0
7	33.3545	34.0	34.0	34.0	34.0	34.0
8	33.428	34.0	34.0	34.0	34.0	34.0
9	33.451	34.0	34.0	34.0	34.0	34.0
10-11	33.462500000000006	34.0	34.0	34.0	34.0	34.0
12-13	33.469	34.0	34.0	34.0	34.0	34.0
14-15	33.446749999999994	34.0	34.0	34.0	34.0	34.0
16-17	33.45025	34.0	34.0	34.0	34.0	34.0
18-19	33.445	34.0	34.0	34.0	34.0	34.0
20-21	33.492000000000004	34.0	34.0	34.0	34.0	34.0
22-23	33.503249999999994	34.0	34.0	34.0	34.0	34.0
24-25	33.5025	34.0	34.0	34.0	34.0	34.0
26-27	33.53725	34.0	34.0	34.0	34.0	34.0
28-29	33.5125	34.0	34.0	34.0	34.0	34.0
30-31	33.476749999999996	34.0	34.0	34.0	34.0	34.0
32-33	33.42825	34.0	34.0	34.0	34.0	34.0
34-35	33.522999999999996	34.0	34.0	34.0	34.0	34.0
36-37	33.501	34.0	34.0	34.0	34.0	34.0
38-39	33.46275	34.0	34.0	34.0	34.0	34.0
40-41	33.57025	34.0	34.0	34.0	34.0	34.0
42-43	33.533249999999995	34.0	34.0	34.0	34.0	34.0
44-45	33.544250000000005	34.0	34.0	34.0	34.0	34.0
46-47	33.50175	34.0	34.0	34.0	34.0	34.0
48-49	33.45125	34.0	34.0	34.0	34.0	34.0
50-51	33.4625	34.0	34.0	34.0	34.0	34.0
52-53	33.43575	34.0	34.0	34.0	34.0	34.0
54-55	33.4535	34.0	34.0	34.0	34.0	34.0
56-57	33.42275	34.0	34.0	34.0	34.0	34.0
58-59	33.494249999999994	34.0	34.0	34.0	34.0	34.0
60-61	33.4435	34.0	34.0	34.0	34.0	34.0
62-63	33.254999999999995	34.0	34.0	34.0	34.0	34.0
64-65	33.3515	34.0	34.0	34.0	34.0	34.0
66-67	33.48350000000001	34.0	34.0	34.0	34.0	34.0
68-69	33.460750000000004	34.0	34.0	34.0	34.0	34.0
70-71	33.4055	34.0	34.0	34.0	34.0	34.0
72-73	33.31675	34.0	34.0	34.0	34.0	34.0
74-75	33.4315	34.0	34.0	34.0	34.0	34.0
76-77	33.4225	34.0	34.0	34.0	34.0	34.0
78-79	33.416250000000005	34.0	34.0	34.0	34.0	34.0
80-81	33.42025	34.0	34.0	34.0	34.0	34.0
82-83	33.4785	34.0	34.0	34.0	34.0	34.0
84-85	33.356750000000005	34.0	34.0	34.0	34.0	34.0
86-87	33.48325	34.0	34.0	34.0	34.0	34.0
88-89	33.394000000000005	34.0	34.0	34.0	34.0	34.0
90-91	33.41375	34.0	34.0	34.0	34.0	34.0
92-93	33.37425	34.0	34.0	34.0	34.0	34.0
94-95	33.3765	34.0	34.0	34.0	34.0	34.0
96-97	33.317	34.0	34.0	34.0	34.0	34.0
98-99	33.32275	34.0	34.0	34.0	34.0	34.0
100-101	33.07	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	3.0
25	1.0
26	9.0
27	19.0
28	32.0
29	42.0
30	76.0
31	118.0
32	250.0
33	2630.0
34	819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.05	14.899999999999999	8.15	21.9
2	24.4	13.850000000000001	31.95	29.799999999999997
3	21.375	23.9	27.85	26.875
4	22.325	27.250000000000004	27.675	22.75
5	22.25	36.175000000000004	24.375	17.2
6	23.549999999999997	31.95	27.525	16.975
7	16.075	27.35	39.425	17.150000000000002
8	16.900000000000002	29.575000000000003	36.175000000000004	17.349999999999998
9	18.0	27.675	34.425	19.900000000000002
10-11	21.712500000000002	32.45	28.675	17.1625
12-13	20.05	28.675	32.574999999999996	18.7
14-15	20.05	27.762500000000003	31.0125	21.175
16-17	20.9875	29.1125	29.5375	20.3625
18-19	21.946946946946948	28.303303303303302	29.817317317317315	19.93243243243243
20-21	19.275000000000002	28.962500000000002	30.6375	21.125
22-23	19.2375	28.9875	30.55	21.224999999999998
24-25	21.6625	27.6375	30.5	20.200000000000003
26-27	19.287499999999998	28.9	29.099999999999998	22.7125
28-29	19.8	27.925	30.337500000000002	21.9375
30-31	18.862499999999997	29.062500000000004	31.587500000000002	20.4875
32-33	19.04104430776955	29.835571733400272	29.546880883645038	21.576503075185137
34-35	21.8875	29.262500000000003	31.3	17.549999999999997
36-37	21.65	28.1125	29.675	20.5625
38-39	20.217826740110166	27.40360540811217	32.473710565848776	19.90485728592889
40-41	22.075	27.1125	30.1375	20.674999999999997
42-43	21.0375	29.475	29.4875	20.0
44-45	22.875	28.125	27.3875	21.6125
46-47	20.8125	28.787499999999998	30.4625	19.9375
48-49	21.1125	29.462500000000002	30.15	19.275000000000002
50-51	20.4625	29.262500000000003	28.0625	22.2125
52-53	20.225	29.65	29.362500000000004	20.7625
54-55	21.2875	28.999999999999996	28.9125	20.8
56-57	19.579105599398723	28.57321808843793	31.278967806589	20.568708505574346
58-59	20.849999999999998	28.999999999999996	30.4	19.75
60-61	20.367591897974492	28.507126781695426	30.532633158289574	20.59264816204051
62-63	21.14199471764558	29.39252924160483	29.39252924160483	20.072946799144763
64-65	21.084941117514408	28.46404409922325	30.93209721874217	19.51891756452017
66-67	21.2625	28.95	29.3375	20.45
68-69	22.1055263815954	27.369342335583895	27.79444861215304	22.73068267066767
70-71	19.15	28.925	30.525000000000002	21.4
72-73	18.89862327909887	28.92365456821026	30.750938673341675	21.426783479349186
74-75	19.625	28.787499999999998	29.95	21.637500000000003
76-77	19.8375	28.325	30.15	21.6875
78-79	20.7625	30.599999999999998	28.775000000000002	19.8625
80-81	21.3125	28.275	29.5	20.9125
82-83	21.075	28.349999999999998	30.45	20.125
84-85	20.263157894736842	28.93483709273183	30.313283208020046	20.48872180451128
86-87	22.112499999999997	28.812500000000004	29.1125	19.9625
88-89	22.608913370055085	27.478718077115673	28.980971457185777	20.931397095643465
90-91	20.8125	28.537499999999998	29.037499999999998	21.6125
92-93	22.525000000000002	28.825	29.375	19.275000000000002
94-95	22.2	29.5	28.549999999999997	19.75
96-97	20.56133316626989	28.630497431399576	29.783235183560958	21.024934218769577
98-99	20.7375	30.837500000000002	28.1375	20.2875
100-101	20.228098759243014	29.953628274219827	30.066424363955385	19.751848602581777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	5.0
18	10.0
19	11.0
20	9.0
21	10.5
22	12.0
23	9.0
24	8.0
25	7.0
26	20.0
27	29.0
28	46.5
29	59.5
30	62.5
31	83.5
32	93.0
33	90.0
34	96.0
35	102.0
36	106.0
37	108.5
38	112.5
39	170.5
40	218.0
41	222.5
42	248.0
43	254.5
44	236.0
45	196.0
46	187.5
47	199.0
48	165.5
49	152.5
50	150.0
51	130.5
52	103.0
53	68.0
54	55.5
55	45.5
56	38.5
57	31.0
58	11.0
59	6.0
60	7.5
61	8.0
62	2.5
63	0.0
64	0.0
65	0.0
66	0.0
67	0.5
68	0.5
69	0.0
70	0.5
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.1
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.41250000000000003
34-35	0.0
36-37	0.0
38-39	0.15
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.21250000000000002
58-59	0.0
60-61	0.025
62-63	0.6125
64-65	0.22499999999999998
66-67	0.0
68-69	0.025
70-71	0.0
72-73	0.125
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.25
86-87	0.0
88-89	0.15
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.2375
98-99	0.0
100-101	0.2625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.511720983419096	19.900000000000002
2	24.299599771297885	21.25
3	12.69296740994854	16.650000000000002
4	7.489994282447113	13.100000000000001
5	4.230989136649514	9.25
6	1.9439679817038307	5.1
7	1.8867924528301887	5.775
8	0.45740423098913663	1.6
9	0.4002287021154946	1.575
>10	1.0863350485991996	5.800000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCATATTCACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGG	21	0.525	No Hit
GGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAACCAAA	17	0.42500000000000004	No Hit
GGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATATATCT	16	0.4	No Hit
GGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTAGGTCAATAGG	13	0.325	No Hit
GTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGC	12	0.3	No Hit
GTCAGTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACT	12	0.3	No Hit
GGCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGC	12	0.3	No Hit
GCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCGAGCGCTGA	12	0.3	No Hit
GTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGA	11	0.27499999999999997	No Hit
CCAAAGCTTTAATACCCACAAGAATCCATGTGCAGCCCGTCTGGCTCACA	11	0.27499999999999997	No Hit
CCGAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCA	11	0.27499999999999997	No Hit
CTGCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGCACTTG	11	0.27499999999999997	No Hit
CAGGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGA	11	0.27499999999999997	No Hit
GCTCTGTGCTGCTCTATGCATATGGCATGTTGTTGGTACCTGTACCATTC	11	0.27499999999999997	No Hit
GCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACAT	11	0.27499999999999997	No Hit
GCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCT	10	0.25	No Hit
ACCGACTATGCATCAGAATACTATGTACAGTTGAAGTTAGGGAGCTGAGA	10	0.25	No Hit
GTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAAGA	10	0.25	No Hit
AAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAATTG	10	0.25	No Hit
GAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAAT	9	0.22499999999999998	No Hit
GTCCATATGAGGGATTAATTGTTCTCATTATAAAGTTTATAATTTCTGCC	9	0.22499999999999998	No Hit
GGGTCCCTTGCCTTTCAATTTTGGCTTTTCCAATAGGAATCAGGAAATCT	9	0.22499999999999998	No Hit
AGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTT	9	0.22499999999999998	No Hit
GCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGATGGGGGTGTCGAT	9	0.22499999999999998	No Hit
GCCAGTTTCTGCTGTGTGGTGAGAGCAGTGTATAAAGACAAGGCGCCTCC	9	0.22499999999999998	No Hit
GCACAAGAAAACTAAGTTCCTCCTGCAACATCCAAGCAGCTTGTCCATCT	9	0.22499999999999998	No Hit
GCCATTCTTCACTTCTTGATCTATCAAGGCTTTTACGGTTTCTGCTGCCT	8	0.2	No Hit
GTGAAAACGAAGCCCGAAGTGGAAGCCAGCAACTGAGTGCAGTGACACCA	8	0.2	No Hit
AGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGAGCAA	8	0.2	No Hit
GCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTA	8	0.2	No Hit
GCTTGACATCCATCATTTCCTGCTGACATGAGCTGTGCATCATGCCTTCA	8	0.2	No Hit
GCTCACCAAGTATGATGGTTTCATACCCAGAAAAACATTTGTTCTTTTGG	8	0.2	No Hit
TGGTACTGCAGTATAGCAAATACTCCACACACTGTGCTTTGAGCTAGAGC	8	0.2	No Hit
TTGCAAAGATGTTGTGTTAGAAATGGTATAAAAACCCAGTTAAACCATTC	8	0.2	No Hit
TCCACTTCATTTCATCACAAATTGTAACTTACTTAACTGACCAAAAAAAC	7	0.17500000000000002	No Hit
GTTGGCTGTAACCATAAAATTGGCAGTTGTATTTACAAATAATCACATAT	7	0.17500000000000002	No Hit
ATTATGTTTTTATCCAATTCTACCCACATGCTGCTGTTTTACTTCTAATG	7	0.17500000000000002	No Hit
GGGAAGGGGAGACCTGAAAGCAAATTTAGATTTAATTGAGAAACATGTTC	7	0.17500000000000002	No Hit
GTTGAAGTTAGGGAGCTGAGAACCTAGTGGTCTGTTCTCTTCTGGTTTGT	7	0.17500000000000002	No Hit
GAGAGAAACTCAAGAATGTCACCGACTATGCATCAGAATACTATGTACAG	7	0.17500000000000002	No Hit
TTTGAAATAGTTGTACAGGATCTTCGCTGAGGTTGTTTTTCCTACCATCT	7	0.17500000000000002	No Hit
GAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTT	7	0.17500000000000002	No Hit
CCTAAGTGAGCAAAAAGAGGTTTCAGAACCAAGGGTCTGGAACAGCCTGA	7	0.17500000000000002	No Hit
GGAGAGAGAAACTCAAGAATGTCACCGACTATGCATCAGAATACTATGTA	7	0.17500000000000002	No Hit
GGCTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	7	0.17500000000000002	No Hit
GTTAGGTCAATAGGTTGAGTTGGATCAACTCGACCTAAATCAATGAGATA	7	0.17500000000000002	No Hit
GGACTGGCAGGTTTGCATCCGAGGCCAGGCACCGTGATGTCCGGGTGATA	7	0.17500000000000002	No Hit
GTTTTGCACAAAATAACCATTTGGAATTTGCAGTGGAATAATTTGTTAAT	7	0.17500000000000002	No Hit
GGGTCATAGAAGGCTGTAGTAACAACACCGCCATTTTTTTCAATGGCAGC	7	0.17500000000000002	No Hit
GCTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCCG	7	0.17500000000000002	No Hit
GTTGGCTGTCCTTAAGAAGAGGGGGAAATCCACTTTGTATTCACTGCCCA	7	0.17500000000000002	No Hit
GTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAATTTTCTTATCTG	7	0.17500000000000002	No Hit
GCCATTAGTTCAGCCAGTGTCAACATGACTAGTGGTTTCCCAAGCACTTG	7	0.17500000000000002	No Hit
GGATCATATATTTCCCATGAGGGTAACATCATTTAGTCTGTATCATTGGT	7	0.17500000000000002	No Hit
GGCTAATTCTGAAGCCAGCTGCACTTCAATATTAATTTTTGCTTGAAAAG	7	0.17500000000000002	No Hit
CAGGAAGTGTGTAACACTGCTTCTGGCCCTCAGGTCGGGTCGGCAACCGT	7	0.17500000000000002	No Hit
GTTTCAAATAAAATTCCAAATACATTTGTACAATGTTTACAAAGTAAGAG	7	0.17500000000000002	No Hit
GAGCATTCTGAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAA	7	0.17500000000000002	No Hit
CCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTT	7	0.17500000000000002	No Hit
CAGGAGGATGTGACTTTCTTTTTAAATCCCAAACCATGCTTTAAAAAAAT	7	0.17500000000000002	No Hit
CACAGCTTAGTGATGAGGCTTTGGTGTACAGGGAAAGGAGAGACTCAAGA	7	0.17500000000000002	No Hit
GCCTATGTCAGTTTTCTTTAAGAAGAATCTAGGAGGCCATCAAGTTGTTG	7	0.17500000000000002	No Hit
GCCAGGTTGTCTAGATGTATTTATAAACATTTAGTAAAGTTCAGTGTATT	7	0.17500000000000002	No Hit
TCCAAATACATTTGTACAATGTTTACAAAGTAAGAGACAGGACTGGCAGG	7	0.17500000000000002	No Hit
GTTTGTTATCTTATTTTGCTGAATTTGAGACAAAGAATCTAGTTGCTTCA	7	0.17500000000000002	No Hit
GTCTGTATCATTGGTATGTATTTGCAAAACATTTTAACACTGCACACATT	7	0.17500000000000002	No Hit
CCCGCTCTGCAAATTTCTGATCACGGTCACTGGCAACACTGCGGTTGTTG	7	0.17500000000000002	No Hit
GCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGC	6	0.15	No Hit
ATTTAAGTAGAAAATGGTCCATCTATGGAAACACTTGGTGTCCATATGAG	6	0.15	No Hit
GTTGGAATATGTATATCTATATATCTCTATATATATTTACATCAAAGAAT	6	0.15	No Hit
GCTGTGACAGCTGGGTCGATCTGATAGGCATAAAACCACACCTCGCTTCT	6	0.15	No Hit
ACAGCAGAAAATACTTGCTTATTGCTGGAACACGTGACATGTCATCCTAC	6	0.15	No Hit
GGAAAGAGGAAGGAGAAAAGCACAAAATATTTAAGGCAAATTAAGCAAAT	6	0.15	No Hit
GCACAAAATAACCATTTGGAATTTGCAGTGGAATAATTTGTTAATTGTGA	6	0.15	No Hit
TAAGTCTGTACACAGCAGGGAACCTGATTATTCTTCTTTTGTCTGTTTTG	6	0.15	No Hit
CTGTAGGTTTTAGAATTTTCTTATCTGCCATATTCACTACCCAGCCAGGA	6	0.15	No Hit
GCTGTCTGTTTTTCAAAATAAAAACACAACTAATGTGTATGCTCTCATTA	6	0.15	No Hit
GGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTC	6	0.15	No Hit
CAGGCACCGTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACACACC	6	0.15	No Hit
GCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTC	6	0.15	No Hit
GTCATGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTA	6	0.15	No Hit
GGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAATGGAG	6	0.15	No Hit
GAGTATCTTGCCGAAAATCCATGGAAGTCAGAAGAGTGCATCAAGTGCCC	6	0.15	No Hit
GTATCATCTATGTGAATACAAACAGAAAAATACAGACACTGCATGGAGAC	6	0.15	No Hit
ACAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAA	6	0.15	No Hit
GCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAG	6	0.15	No Hit
GCGAGTTCAAGTCTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCC	6	0.15	No Hit
CCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCA	6	0.15	No Hit
AGTCATCTCTGGTTCTCTTACTGAGAATAGAAAGTCTCTCCTTACTGAGA	6	0.15	No Hit
GGAAGGACGTAGCCAAACTTCATGGCGAGTTCAAGTCTTGCTTCAGGAAA	6	0.15	No Hit
GACACTTAAAAGGATCATATATTTCCCATGAGGGTAACATCATTTAGTCT	6	0.15	No Hit
CTGAGCAAGTTGTACAGCTAAAGTGCCCTTTTGGGGGAATTGGTCACACA	6	0.15	No Hit
GGCTTTTCCAATAGGAATCAGGAAATCTTTTGACTGAGCTTCTGCAGGAA	6	0.15	No Hit
GTAGACAAACACAGCTTAGTGATGAGGCTTTGGTGTACAGGGAAAGGAGA	6	0.15	No Hit
CAAGAATCCATGTGCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAG	6	0.15	No Hit
TGGGAAGAGGTTTATACTCTACTACTGTGATGCTGGTGAACTTCTACTCA	6	0.15	No Hit
GGACAAAGGGAAAAAAGGGCCATTTATAGGTCTCAAGAGCAGACCCTGCA	6	0.15	No Hit
GCTGACATGAGCTGTGCATCATGCCTTCATAGATTTTGAAGGTTACATTG	6	0.15	No Hit
GAGGAAAATTATTCATTATGAAGTACTACTTTCCACTTCATTTCATCACA	6	0.15	No Hit
CTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGGTCACA	6	0.15	No Hit
CTCTACTACTGTGATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATG	6	0.15	No Hit
GTCAGCCAGGTAGCCCCGGTTTTTTGCATCGGTGTAATAGGGCACCAGTG	5	0.125	No Hit
CTTGCATTTGTGAGGCATTTCTAGAGATGATGTCCAGTGGGCAGCATCCA	5	0.125	No Hit
AGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCGCAGAAA	5	0.125	No Hit
CTCATTAAGGCTAACTATGCTATTCTCCACTGCGCCCCATTGATCAGACA	5	0.125	No Hit
CCTGTACCATTCTCTTCCATTGAGAATTCTAAGCCATTATGTGGAGGAGC	5	0.125	No Hit
CACTAAAAAAAATTTACCTCTCTAAACATTTACTTCACTCATGGACACAA	5	0.125	No Hit
GCCAGCAACTGAGTGCAGTGACACCAGCCAGTTTCTGCTGTGTGGTGAGA	5	0.125	No Hit
GCATCGGTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGG	5	0.125	No Hit
AGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGT	5	0.125	No Hit
GGCAAAGTAAGTTTTGAGGTATAGAGATAGAGTAGGGATGGGGACCCAGG	5	0.125	No Hit
GTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTAGAAT	5	0.125	No Hit
CTTCATTTCATCACAAATTGTAACTTACTTAACTGACCAAAAAAACTATG	5	0.125	No Hit
GCCCTCTTCAACCAGCTGGACTCCATAATCCCTCTTAAGCGGCTGGATGG	5	0.125	No Hit
GCAGCAAGTGCTTGCGCTATCACAACCTTGTCATTTGTTTGTTGGGTTCT	5	0.125	No Hit
GTCTGATTTAGTAGAAGGCAGTTCAGGAGCTGTAATACTTGAGAAGATTC	5	0.125	No Hit
GTCCATTGACAACTTAATTTTAGAGAATCAGAGGAGATTATATTGGGTGT	5	0.125	No Hit
GTGATGTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCT	5	0.125	No Hit
GGTTGGATTTATCAATGCTTTTAGTCTTTCAACAGTAAGAGAACCAAACA	5	0.125	No Hit
CTGCAAATTTCTGATCACGGTCACTGGCAACACTGCGGTTGTTGGAGATG	5	0.125	No Hit
GGTCAGCAAAGAACGGAAGATCGTGTGGGACTGTAGTTCCAGTTTTGAGT	5	0.125	No Hit
CCTGTATTATTTCAGAGACCAAATCTGGCTTACTGGAGGTTCCAAAAAAG	5	0.125	No Hit
GTTGCTTCACTTACCTACTGTAATACTTAGTATATTCTGAATTGTATAAT	5	0.125	No Hit
GCATTTATTCTTGAGGTTACTGAGCCATCTGGCTACAGAGAAGAAAAGCA	5	0.125	No Hit
AGTCATCTCTCCATCCCCACCTTAACTCCTTTGATTGAGGGGTGGTATGG	5	0.125	No Hit
ATGCTGGTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGA	5	0.125	No Hit
CTCTCCACCACCTCCTGCTAGTCATACCAAACCCACCCAAAACTAGACCA	5	0.125	No Hit
GGCAGTTCAGGAGCTGTAATACTTGAGAAGATTCTCATCTGTAGGTTTTA	5	0.125	No Hit
GTCATTTTCTAATGTCTGCCTAAAGAGAGAAAGATGCTCTCGCCTGAGTT	5	0.125	No Hit
GTTTACAAAGTAAGAGACAGGACTGGCAGGTTTGCATCCGAGGCCAGGCA	5	0.125	No Hit
CACATATGCTAAAAGCTAGGTTTTTCTAATACACTTTTATTTTCATGGAG	5	0.125	No Hit
CGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATTT	5	0.125	No Hit
GTAGGGATGGGGACCCAGGTGTGTACAGATTCCTTGCTTCTTTGTTGCTA	5	0.125	No Hit
TGCTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCC	5	0.125	No Hit
GGCCCCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGGTTGTCCCCG	5	0.125	No Hit
AGCAATTGTCAAAGGGTTAAAGCCTCTTTAACACTTGCATGGGTGTGAGA	5	0.125	No Hit
ATAGAATGTTCTCTCCACCACCTCCTGCTAGTCATACCAAACCCACCCAA	5	0.125	No Hit
CCCTACTGTTAGCACAGTTCAATGGTGCATTCTTATTTTATAATATTGAC	5	0.125	No Hit
GTGCTTTACCAGCTTTCACATTCCTTATAACACACTGTGAGGTAGGTGAA	5	0.125	No Hit
CCTCTTAAGCGGCTGGATGGTCACACCTCTCCCATTCACAAGCTGGGTTA	5	0.125	No Hit
TCATTATGAAGTACTACTTTCCACTTCATTTCATCACAAATTGTAACTTA	5	0.125	No Hit
CTGTGATGCACACGGTTCTCCATGTTCCTGCAATTTAGTGAGAGGCCTGG	5	0.125	No Hit
CTTTTATTTTCATGGAGGGCTCTAGAGGATTCCACGTGGAATAGAAACCA	5	0.125	No Hit
CAGCAGAATATCAATGATAAAATTATGAAACATAGCAAAAGGCTAAAAGA	5	0.125	No Hit
GAATGGAATAGGCTTGCACAGAATTTCTAGACTTCTTGGGTCATAGAAGG	5	0.125	No Hit
GCTAGAGCACTTGGAGTCACTGCCCAGGGCAGCAGCCTCCAACCAAGAAA	5	0.125	No Hit
GCCCAGACTCTTGCCATCTGGAAGCAGGCTCACCAAGTATGATGGTTTCA	5	0.125	No Hit
TGTGATTTCAGATGCAATTTGAATTATTGAAGGAGACGTGGAGAGAATAT	5	0.125	No Hit
AATAACCATTTGGAATTTGCAGTGGAATAATTTGTTAATTGTGAAGTGTA	5	0.125	No Hit
GAAGAGTTCATCTTTCGTAATATCAGGAAGGACGTAGCCAAACTTCATGG	5	0.125	No Hit
GTAGAAAAAATATTGGTTTCAGAAGGAGATAAAAAGTCAGGAATATTCAA	5	0.125	No Hit
TCGGAATTGGTTGTCCCCGCAGAAAGAATGGAATAGGCTTGCACAGAATT	5	0.125	No Hit
GCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGCATC	5	0.125	No Hit
GCCAGGCACTCTATTTGAGTAGATCAACATCTTTGCCAGCCAGCAGACCA	5	0.125	No Hit
CAGTGGAATAATTTGTTAATTGTGAAGTGTATGGTTTTGTATTTTGAAAC	5	0.125	No Hit
TTTTTTGAAAATTGTTTTGCACAAAATAACCATTTGGAATTTGCAGTGGA	5	0.125	No Hit
GTGTAATAGGGCACCAGTGACTCAGGGGGAAGCATTCTTTTCGGAATTGG	5	0.125	No Hit
GCTGCACCAACAGCAATTCCAGCAAAGCCCATCTCTGAGATGGGGGTGTC	5	0.125	No Hit
CTTGAAAAGTATCAGCACCCTCTTCAACCAGCTGGACTCCATAATCCCTC	5	0.125	No Hit
GTGGGACTGTAGTTCCAGTTTTGAGTACCATATGGATACTGAGACTTGAA	5	0.125	No Hit
CCATTCACAAGCTGGGTTAGGTCAATAGGTTGAGTTGGATCAACTCGACC	5	0.125	No Hit
GTGAACTTCTACTCAAGGCCTCTTAGTGATGTCAATCAATTGGAGGTAGG	5	0.125	No Hit
CACTACCCAGCCAGGAGCAAGACCAAAGAAAATCTGCCTCGGATCCTTCC	5	0.125	No Hit
GTCCGGGTGATATATCTTCACGTTGCCTGCACACACCTTATTTCTGAAAT	5	0.125	No Hit
CTTGCTTCAGGAAACTTGGCAGGGTCAGCCAGGTAGCCCCGGTTTTTTGC	5	0.125	No Hit
GTTTGTTTGTTTTCTTTTCTTTTGTTTTAGGACAGGATTTTAAGTGTAGC	5	0.125	No Hit
CTTCAGATTCACCTTCCACAGTTTTTAGTTACTGGTTGGTGGCCATCCAC	5	0.125	No Hit
GACCAAAAAAACTATGGTACTGCAGTATAGCAAATACTCCACACACTGTG	5	0.125	No Hit
GGCATGTGAGAGTAAGTCACATGGTAGGAGGCTGCCTTTCTATCATTCAA	5	0.125	No Hit
TCTCATTAAGGCTAACTATGCTATTCTCCACTGCGCCCCATTGATCAGAC	5	0.125	No Hit
GGGGGGGGTTTGCAAGGGCAAAAAGGACGGAGGGATTGTGGATCTGGAGT	5	0.125	No Hit
GCAGCCCGTCTGGCTCACAAGTCTGATTTAGTAGAAGGCAGTTCAGGAGC	5	0.125	No Hit
GTATGGTTTTGTATTTTGAAACCAAACAACAGTAGCTGAGGTAGTTAAAT	5	0.125	No Hit
ACAAATTGTAACTTACTTAACTGACCAAAAAAACTATGGTACTGCAGTAT	5	0.125	No Hit
CACACATTAGAAGTTCTATGGTCAGTCATGGGAAGAGGTTTATACTCTAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.037500000000000006	0.0	0.0	0.0	0.0
82-83	0.05	0.0	0.0	0.0	0.0
84-85	0.1	0.0	0.0	0.0	0.0
86-87	0.16249999999999998	0.0	0.0	0.0	0.0
88-89	0.175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR32570290 read2 length is 101 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32570290_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.0375	34.0	34.0	34.0	34.0	34.0
2	33.2365	34.0	34.0	34.0	34.0	34.0
3	32.9415	34.0	34.0	34.0	34.0	34.0
4	33.181	34.0	34.0	34.0	34.0	34.0
5	33.1335	34.0	34.0	34.0	34.0	34.0
6	33.1755	34.0	34.0	34.0	34.0	34.0
7	33.2805	34.0	34.0	34.0	34.0	34.0
8	33.3185	34.0	34.0	34.0	34.0	34.0
9	33.319	34.0	34.0	34.0	34.0	34.0
10-11	33.288	34.0	34.0	34.0	34.0	34.0
12-13	33.275	34.0	34.0	34.0	34.0	34.0
14-15	33.264250000000004	34.0	34.0	34.0	34.0	34.0
16-17	33.1945	34.0	34.0	34.0	34.0	34.0
18-19	33.3	34.0	34.0	34.0	34.0	34.0
20-21	33.326499999999996	34.0	34.0	34.0	34.0	34.0
22-23	33.30375	34.0	34.0	34.0	34.0	34.0
24-25	33.2335	34.0	34.0	34.0	34.0	34.0
26-27	33.30575	34.0	34.0	34.0	34.0	34.0
28-29	33.20475	34.0	34.0	34.0	34.0	34.0
30-31	33.29375	34.0	34.0	34.0	34.0	34.0
32-33	33.28825	34.0	34.0	34.0	34.0	34.0
34-35	33.28525	34.0	34.0	34.0	34.0	34.0
36-37	33.2875	34.0	34.0	34.0	34.0	34.0
38-39	33.2375	34.0	34.0	34.0	34.0	34.0
40-41	33.32325	34.0	34.0	34.0	34.0	34.0
42-43	33.255250000000004	34.0	34.0	34.0	34.0	34.0
44-45	33.301	34.0	34.0	34.0	34.0	34.0
46-47	33.3095	34.0	34.0	34.0	34.0	34.0
48-49	33.2835	34.0	34.0	34.0	34.0	34.0
50-51	33.3035	34.0	34.0	34.0	34.0	34.0
52-53	33.22725	34.0	34.0	34.0	34.0	34.0
54-55	33.23975	34.0	34.0	34.0	34.0	34.0
56-57	33.28575	34.0	34.0	34.0	34.0	34.0
58-59	33.297250000000005	34.0	34.0	34.0	34.0	34.0
60-61	33.294250000000005	34.0	34.0	34.0	34.0	34.0
62-63	33.248	34.0	34.0	34.0	34.0	34.0
64-65	33.21225	34.0	34.0	34.0	34.0	34.0
66-67	33.24375	34.0	34.0	34.0	34.0	34.0
68-69	33.248000000000005	34.0	34.0	34.0	34.0	34.0
70-71	33.22175	34.0	34.0	34.0	34.0	34.0
72-73	33.303250000000006	34.0	34.0	34.0	34.0	34.0
74-75	33.210750000000004	34.0	34.0	34.0	34.0	34.0
76-77	33.06925	34.0	34.0	34.0	34.0	34.0
78-79	33.19	34.0	34.0	34.0	34.0	34.0
80-81	33.2365	34.0	34.0	34.0	34.0	34.0
82-83	33.22725	34.0	34.0	34.0	34.0	34.0
84-85	33.1945	34.0	34.0	34.0	34.0	34.0
86-87	33.2065	34.0	34.0	34.0	34.0	34.0
88-89	33.06975	34.0	34.0	34.0	34.0	34.0
90-91	33.1275	34.0	34.0	34.0	34.0	34.0
92-93	33.03875	34.0	34.0	34.0	34.0	34.0
94-95	33.14725	34.0	34.0	34.0	34.0	34.0
96-97	33.1345	34.0	34.0	34.0	34.0	34.0
98-99	33.105999999999995	34.0	34.0	34.0	34.0	34.0
100-101	32.8625	34.0	34.0	34.0	34.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	4.0
23	5.0
24	12.0
25	19.0
26	18.0
27	32.0
28	30.0
29	53.0
30	68.0
31	136.0
32	295.0
33	2872.0
34	453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.775	24.9	10.424999999999999	15.9
2	21.65	28.349999999999998	30.75	19.25
3	19.825	29.799999999999997	32.1	18.275
4	23.275000000000002	34.925	24.8	17.0
5	22.75	38.324999999999996	21.575	17.349999999999998
6	20.549999999999997	35.3	24.375	19.775000000000002
7	17.7	26.825	36.475	19.0
8	20.325	26.55	28.775000000000002	24.349999999999998
9	19.575	29.4	27.750000000000004	23.275000000000002
10-11	22.075	32.5125	25.5375	19.875
12-13	18.75	29.2375	27.8625	24.15
14-15	20.1625	29.762499999999996	29.299999999999997	20.775
16-17	21.481296134117354	29.20055048167146	27.624171149756037	21.69398223445515
18-19	21.4	29.8875	27.9375	20.775
20-21	20.4	30.7625	28.5625	20.275000000000002
22-23	21.85	29.6375	25.387500000000003	23.125
24-25	20.5625	30.412499999999998	28.1375	20.8875
26-27	19.8625	30.362499999999997	28.525	21.25
28-29	20.588603631809644	30.381966186599875	27.48904195366312	21.540388227927366
30-31	22.3625	29.225	27.875	20.5375
32-33	20.4375	30.9625	26.7125	21.8875
34-35	22.237499999999997	28.537499999999998	28.175	21.05
36-37	20.3875	29.525000000000002	28.925	21.1625
38-39	22.8875	27.8625	27.8875	21.3625
40-41	19.950000000000003	30.925000000000004	28.050000000000004	21.075
42-43	20.037546933667084	29.86232790988736	27.972465581977474	22.127659574468083
44-45	21.3875	29.4375	28.212500000000002	20.962500000000002
46-47	20.25	30.375000000000004	27.762500000000003	21.6125
48-49	22.382264529058116	28.95791583166333	27.229458917835668	21.430360721442888
50-51	20.575	31.025000000000002	27.3875	21.0125
52-53	22.125	29.75	28.3375	19.787499999999998
54-55	20.9875	30.15	26.1125	22.75
56-57	20.7375	29.3875	28.9375	20.9375
58-59	19.7	29.45	28.812500000000004	22.037499999999998
60-61	21.4	29.65	26.375	22.575
62-63	19.950000000000003	28.825	30.825000000000003	20.4
64-65	20.875	28.3125	28.5625	22.25
66-67	22.037499999999998	29.375	28.15	20.4375
68-69	20.65	29.262500000000003	28.275	21.8125
70-71	21.212500000000002	29.375	28.0875	21.325
72-73	21.3625	27.85	28.65	22.1375
74-75	20.5875	29.049999999999997	28.725	21.637500000000003
76-77	21.67919799498747	29.786967418546368	27.56892230576441	20.964912280701757
78-79	22.0125	28.3875	29.375	20.225
80-81	21.589486858573217	28.635794743429287	29.499374217772218	20.275344180225282
82-83	21.79952446502315	28.444500062570395	28.03153547741209	21.72443999499437
84-85	19.412499999999998	29.475	30.025000000000002	21.087500000000002
86-87	20.0875	28.625	30.9625	20.325
88-89	20.833333333333336	30.305305305305307	27.239739739739736	21.62162162162162
90-91	20.065089498059834	28.55175866816873	30.516960821129054	20.866191012642382
92-93	21.320305649505197	29.036702993861958	28.222472754603533	21.42051860202931
94-95	21.9625	30.0	27.8375	20.200000000000003
96-97	21.3125	29.5375	28.050000000000004	21.099999999999998
98-99	20.9125	30.7375	28.15	20.200000000000003
100-101	21.475	29.7375	27.250000000000004	21.5375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.5
18	5.5
19	1.0
20	0.5
21	5.0
22	7.0
23	6.5
24	9.5
25	11.5
26	13.0
27	27.0
28	35.5
29	45.0
30	62.5
31	61.0
32	74.0
33	90.0
34	79.5
35	77.5
36	92.0
37	112.5
38	149.5
39	170.5
40	194.0
41	239.0
42	258.5
43	253.0
44	251.5
45	215.0
46	198.0
47	217.5
48	197.0
49	167.0
50	131.5
51	118.0
52	108.0
53	84.0
54	65.5
55	45.5
56	32.0
57	24.0
58	16.5
59	10.5
60	12.5
61	11.0
62	3.0
63	1.0
64	1.0
65	1.0
66	1.0
67	0.5
68	1.0
69	0.5
70	0.5
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.08750000000000001
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.1875
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.125
44-45	0.0
46-47	0.0
48-49	0.2
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.25
78-79	0.0
80-81	0.125
82-83	0.11249999999999999
84-85	0.0
86-87	0.0
88-89	0.1
90-91	0.13749999999999998
92-93	0.21250000000000002
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.57929883138564	21.375
2	23.76182526432944	21.349999999999998
3	11.908736783528102	16.05
4	7.512520868113523	13.5
5	3.672787979966611	8.25
6	1.8363939899833055	4.95
7	1.5025041736227045	4.725
8	0.8903728436282694	3.2
9	0.27824151363383415	1.125
>10	1.05731775180857	5.475
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAGATTGTGACCCTTTAGTTCCCCTAATGTTTGGTTCTCTTACTGTTGA	17	0.42500000000000004	No Hit
GAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTTCTT	14	0.35000000000000003	No Hit
GCTACGTCCTTCCTGATATTACGAAAGATGAACTCTTCAGAATGCTCAGC	13	0.325	No Hit
GGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCT	13	0.325	No Hit
GCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGT	12	0.3	No Hit
GGTAGATGAAGACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCC	12	0.3	No Hit
GACCAATTCCCCCAAAAGGGCACTTTAGCTGTACAACTTGCTCTGAGGCA	12	0.3	No Hit
ACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATTCTGATGCAT	12	0.3	No Hit
GGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTG	11	0.27499999999999997	No Hit
GGTGGAGCAACACGAGGCCTCCACACAGTGTAAAACCTAAACAGTGCTGG	11	0.27499999999999997	No Hit
GAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGTATT	11	0.27499999999999997	No Hit
GCAGACTCCATTTTACATACGAATCCCAAAATATGGATTTAATGAAGGAC	11	0.27499999999999997	No Hit
GAATATGGCAGATAAGAAAATTCTAAAACCTACAGATGAGAATCTTCTCA	10	0.25	No Hit
GCATAGTTAGCCTTAATGAGAATCTTGCTTCTACTTAAAAAAATAAAAAT	10	0.25	No Hit
GGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAACCT	10	0.25	No Hit
GAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAA	10	0.25	No Hit
GTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTC	10	0.25	No Hit
GAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACT	10	0.25	No Hit
AGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTA	10	0.25	No Hit
GAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCATTTTAC	9	0.22499999999999998	No Hit
TTAAATTTATCTCTGAGCAAAAGAGGAGTAATAGCTGGTAGATGAAGACA	9	0.22499999999999998	No Hit
AGTATATCCTTTTTATCATAAAAATAAACATCGGTTTTGATTCAGTTTTA	9	0.22499999999999998	No Hit
GGCATCCAAAAGAACAAATGTTTTTCTGGGTATGAAACCATCATACTTGG	9	0.22499999999999998	No Hit
GTCATGTTGACACTGGCTGAACTAATGGCATCCAAAAGAACAAATGTTTT	9	0.22499999999999998	No Hit
CCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGA	8	0.2	No Hit
GAGGCTGCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAG	8	0.2	No Hit
GAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCC	8	0.2	No Hit
CATAGTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTA	8	0.2	No Hit
GATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATT	8	0.2	No Hit
AGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCG	8	0.2	No Hit
GGTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGA	8	0.2	No Hit
GCCATTGAAAAAAATGGCGGTGTTGTTACTACAGCCTTCTATGACCCAAG	8	0.2	No Hit
AAGAGGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCA	8	0.2	No Hit
AGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAACTGTACATAGT	8	0.2	No Hit
ACCAGACCTTCCTTTAGAATTCAACTTGTAAGTGTGTCTTTCTTGGTTGG	8	0.2	No Hit
ATACATCTAGACAACCTGGCCCTTTCTAATCCCTAAGGACTTTGTATGGC	8	0.2	No Hit
GTGTGACCATCCAGCCGCTTAAGAGGGATTATGGAGTCCAGCTGGTTGAA	8	0.2	No Hit
GGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGT	8	0.2	No Hit
GTTTCACATTTAACTACCTCAGCTACTGTTGTTTGGTTTCAAAATACAAA	8	0.2	No Hit
GCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTC	8	0.2	No Hit
GATATATAGATATACATATTCCAACCCCACATAACCAGACCTTCCTTTAG	7	0.17500000000000002	No Hit
TAAACACAAGGGTCGGTAGGAAGAACAGAGGCCCATTAACAATCAAGCCA	7	0.17500000000000002	No Hit
CAAGAAGTTGGTCATACAAGCAGCACATATGGTTTTGGGATAGCACACAT	7	0.17500000000000002	No Hit
TGGAATTTTATTTGAAACAAAAATTTAAATGAGTTATTAAATTCTTTGAT	7	0.17500000000000002	No Hit
TTGAGGGAGGGCAGACTCCATTTTACATACGAATCCCAAAATATGGATTT	7	0.17500000000000002	No Hit
CTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCAACGTGA	7	0.17500000000000002	No Hit
GATATACATATTCCAACCCCACATAACCAGACCTTCCTTTAGAATTCAAC	7	0.17500000000000002	No Hit
GTCAACCATATTACTGATATATTACCTTCACAAAACCGTTTTCATGAACC	7	0.17500000000000002	No Hit
AGAAGAGTTTTAAGGCTGTCTTGGGTGACTGGATGCATCCACCACTTATT	7	0.17500000000000002	No Hit
ACTGCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCACTCAGTTG	7	0.17500000000000002	No Hit
CCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTGCCAAG	7	0.17500000000000002	No Hit
GCATGATGCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTC	7	0.17500000000000002	No Hit
GTTTCAGGCACCAGTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTC	7	0.17500000000000002	No Hit
GCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATA	7	0.17500000000000002	No Hit
GTTGGACTTTCACCAGATTCCCAGGAAGATGAATCTGGAATTAAACAGGC	7	0.17500000000000002	No Hit
GGTGGAGAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATAAA	7	0.17500000000000002	No Hit
GTATCAGCCTTTGAGTCTCAGGAGACTACAGTATCTCATTGATTTAGGTC	7	0.17500000000000002	No Hit
CCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGAAGTTTGGCTA	7	0.17500000000000002	No Hit
GGAGTAATAGCTGGTAGATGAAGACAAACCAGAAGAGAACAGACCACTAG	7	0.17500000000000002	No Hit
GTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGGAACCCGG	7	0.17500000000000002	No Hit
GAGGGTGCTGATACTTTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGC	7	0.17500000000000002	No Hit
ATTGAAGTGCAGCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAA	7	0.17500000000000002	No Hit
GTTGATCCAACTCAACCTATTGACCTAACCCAGCTTGTGAATGGGAGAGG	7	0.17500000000000002	No Hit
GTCTCTTTTCACAATCTGTACCCAGATGATAGATGACTTAAACACTAGGT	7	0.17500000000000002	No Hit
TACTTTGTAAACATTGTACAAATGTATTTGGAATTTTATTTGAAACAAAA	7	0.17500000000000002	No Hit
GTTACTACAGCCTTCTATGACCCAAGAAGTCTAGAAATTCTGTGCAAGCC	7	0.17500000000000002	No Hit
CTCACTAAATTGCAGGAACATGGAGAACCGTGTGCATCACAGGAAGAAGA	7	0.17500000000000002	No Hit
AGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTGGAGTATTTGCTA	6	0.15	No Hit
AATATTTTGTGCTTTTCTCCTTCCTCTTTCCAATGTTTGTATTTTAAATT	6	0.15	No Hit
GCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATCACTAAGAGG	6	0.15	No Hit
CAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCAAC	6	0.15	No Hit
GTGAACCTCTCAGACAGCCACGGAGCCATTTCCTCCGTGGTGTCCGACGC	6	0.15	No Hit
ACTGCAGGGTGTTGCTGGATGTTTAAAGCTACCCTGAGCTACATAGTGAG	6	0.15	No Hit
CAATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACC	6	0.15	No Hit
GTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAACCTCTTCCCATGA	6	0.15	No Hit
ATGGGCAAAGGGTTGAAGTTTAAACTTTAAAGCGTTTGTGAGCCACACAG	6	0.15	No Hit
GATGTTGATCTACTCAAATAGAGTGCCTGGCTCATCTGTCAAATGATCTG	6	0.15	No Hit
CAAAATACAAAACCATACACTTCACAATTAACAAATTATTCCACTGCAAA	6	0.15	No Hit
GCTCAGGAACCTAGTGTAGGAGTTGGTTAACTGTATAAGTTGGTTAACTG	6	0.15	No Hit
GTACATATTTGCTTAATTTGCCTTAAATATTTTGTGCTTTTCTCCTTCCT	6	0.15	No Hit
GATGAACTCTTCAGAATGCTCAGCGCTCGGAAGGATCCGAGGCAGATTTT	6	0.15	No Hit
GGAGATAGTACTATTAAAATTGGCAGTCACAAGTAACCTGTGGTGGTTAT	6	0.15	No Hit
GTAAGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTGACTCCA	6	0.15	No Hit
GCACTCGCAATGCTTTGCTGCATGGTATGGGCACTGCCCAGGTTTAAAAG	6	0.15	No Hit
GGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACCTGGCTGACCCTG	6	0.15	No Hit
AGTGAGTGTATTTATTCACTGAGACATCTTGCTAGTCCTAGTATTACAAT	6	0.15	No Hit
GCGCTCGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGG	6	0.15	No Hit
TTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCCAG	6	0.15	No Hit
AGGAAGATGAATCTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTG	6	0.15	No Hit
GACTGTACATATTTGCTTAATTTGCCTTAAATATTTTGTGCTTTTCTCCT	6	0.15	No Hit
AAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGAC	6	0.15	No Hit
GTGACAAGAGGGTATGTGGTTCAATGATAGCAACAAAGAAGCAAGGAATC	6	0.15	No Hit
CCAGATTTGGGCAATCACCAAGTTGTGCTTGTGATGATCCAGCCACTGCC	6	0.15	No Hit
GGAAAAAAAGTCAGAAACATGCTAAACTTCCTCACCATCTCAAAGCAAAA	6	0.15	No Hit
ATATATTCCATCCAGGTATAGTGACAAGAGGGTATGTGGTTCAATGATAG	6	0.15	No Hit
GCTATTAATCAAGGTATGGATGAAGAACTAGAAAGAGATGAAAAAGTTTT	6	0.15	No Hit
GCATAGAGCAGCACAGAGCAACATAATATTGCTGGGGGGAAAAAGAGTTG	6	0.15	No Hit
GCAGAGAGGAACCCGGCCAAGGCTGGGCTTTGAGGGAGGGCAGACTCCAT	6	0.15	No Hit
AATTGTCTAATTAGGTTCAACCTAATAGCGTGCCTTGCAGTTAACGATGG	6	0.15	No Hit
GACAGAGTGAGATTCAGATTCTCAGGTGTGTGCATCTTATATTGGAATGC	6	0.15	No Hit
CTCAGGAGACTACAGTATCTCATTGATTTAGGTCGAGTTGATCCAACTCA	5	0.125	No Hit
GCTCTCACCACACAGCAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTG	5	0.125	No Hit
GCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATCG	5	0.125	No Hit
AGTATGACGGTGCATACAAGGTTAGCAGAGGCCTGTGGAAGAAATACGGT	5	0.125	No Hit
ATAAAATCTAAATAGGTTAGAGAGAGCTGAACAGCTCCTCCACATAATGG	5	0.125	No Hit
TTCTGGGTATGAAACCATCATACTTGGTGAGCCTGCTTCCAGATGGCAAG	5	0.125	No Hit
CGGAAGGATCCGAGGCAGATTTTCTTTGGTCTTGCTCCTGGCTGGGTAGT	5	0.125	No Hit
TGTTAGGATAGTCAGGTCATGTAAGAACAGTAAGTGGGTAAAAGGTAATT	5	0.125	No Hit
ATGGACTCAATCAAAGTTTTAAATTTTCAAATTGGCTAAACATTTCTAAT	5	0.125	No Hit
ATTTTCATTTCATGTGACAAATTCCTGAGAAAACAGCTTGAGAAAGGGAA	5	0.125	No Hit
ATCTGTTCTGAAGTTAGAGAAAAGTTACCATATCCTGAGTGACAGGACTT	5	0.125	No Hit
CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGA	5	0.125	No Hit
CTGCTGCTAAGAGTCTCTTTGTTGTACAAAGAGAAATACATCACATAGAG	5	0.125	No Hit
GGCTTCAGCACCATATTGAAATATGGCTACTTGCATAAATAGGATACACA	5	0.125	No Hit
GGTACAGGTACCAACAACATGCCATATGCATAGAGCAGCACAGAGCAACA	5	0.125	No Hit
TGCCAGTCACATTAAATATGAATATGGCTATGCCTTCTTGGTTTGATATC	5	0.125	No Hit
ATATTTGCTTCCAAGCAAGTGCTTGGGAAACCACTAGTCATGTTGACACT	5	0.125	No Hit
GATAAATCCAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACA	5	0.125	No Hit
GTAGGGACCATTTTGTTCCCTATATAAGCATCTTGGTGGCTCTATCAAAG	5	0.125	No Hit
GAGAATCTTGCTTCTACTTAAAAAAATAAAAATAAAATAAAAATAAAAAA	5	0.125	No Hit
GTGGAATGGTGTTTCTGCTCAGAACTCTTGAGCTGATTCATCTCCATATT	5	0.125	No Hit
AGTGTGTCTTTCTTGGTTGGAGGCTGCTGCCCTGGGCAGTGACTCCAAGT	5	0.125	No Hit
TGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTACC	5	0.125	No Hit
GCATAGTCGGTGACATTCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGT	5	0.125	No Hit
CCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAATGCTTCCCCCTGA	5	0.125	No Hit
CAGCCAATGTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGT	5	0.125	No Hit
CTGTCATTGACAGAGGGCCTCTCCTCCTGGATTTCATCTGCATTTTCTTA	5	0.125	No Hit
CCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTCGCCATGA	5	0.125	No Hit
GCTGGCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTG	5	0.125	No Hit
GGCATTCCTTCTAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGC	5	0.125	No Hit
GTGGTGGAGAGAACATTCTATAGAGAGATTCTATCACAAAGTGAAAGATA	5	0.125	No Hit
CTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTACGAAAGA	5	0.125	No Hit
GTTTACCAGCTCTAGAATTTTCCTGATGATTTTAAAGGTCTTTTATGTAT	5	0.125	No Hit
GTCCTCAGTTACCCCAAATACCATGTATATGCACAAGCTGTTTCATTGAA	5	0.125	No Hit
AGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAGAGG	5	0.125	No Hit
GCTGCCCTGGGCAGTGACTCCAAGTGCTCTAGCTCAAAGCACAGTGTGTG	5	0.125	No Hit
GAAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGA	5	0.125	No Hit
GTTGACTATTTGGTCTAGTTTTGGGTGGGTTTGGTATGACTAGCAGGAGG	5	0.125	No Hit
GCTTCAGAATTAGCCATTGCTGCCATTGAAAAAAATGGCGGTGTTGTTAC	5	0.125	No Hit
GGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGATTGACATC	5	0.125	No Hit
AAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTACCTCCAATTGAT	5	0.125	No Hit
GGGCAGAAGCCTTTGCAGGTATCAAAAGTCCCCACATCAAATATATCTGT	5	0.125	No Hit
GTCCCACACAATGGTACTCAAAACTGTAACTACAGTCCCACACGATGGTA	5	0.125	No Hit
GAAACCATCATACTTGGTGAGCCTGCTTCCAGATGGCAAGAGTCTGGGCT	5	0.125	No Hit
GTAACCTTCAAAATCTATGAAGGCATGATGCACAGCTCATGTCAGCAGGA	5	0.125	No Hit
AAAACCTAAACAGTGCTGGAGTTGCACTTCAGCCTGTACAGGCTCTCGGT	5	0.125	No Hit
GTTGACACTGGCTGAACTAATGGCATCCAAAAGAACAAATGTTTTTCTGG	5	0.125	No Hit
GTGGTTTCTGGGGATTGAACTCAGGACCATTGGAAGAGCAGTCAGTGCTC	5	0.125	No Hit
CGGAGAAGAGGTAGGAAGTGTGGCAGAGGCCATAAAGGAGAAAGGCAGAG	5	0.125	No Hit
AAGCTCTATCAGTTATATAGTGAAGGTGAGACAATTTAATAAGCAGCTGT	5	0.125	No Hit
GGGGCTACCTGGCTGACCCTGCCAAGTTTCCTGAAGCAAGACTTGAACTC	5	0.125	No Hit
ACGAATAATAGAATGTCCAGATTAATCCTTGGGTCTTCTTACTCCTAAGT	5	0.125	No Hit
GTTTTCACAGGGGCCGATCAACAGTGCTAATCGAGATATTTCCGTTCTCC	5	0.125	No Hit
AATTCCGAAAAGAATGCTTCCCCCTGAGTCACTGGTGCCCTATTACACCG	5	0.125	No Hit
GTAAATTAATCTCTGCCACTCCATAATTATGGTACTTCACATGTCTTCTT	5	0.125	No Hit
TTAACTACTATCACTGATCTCTACACACAACCTCCAGCTTTAAATGGGAG	5	0.125	No Hit
GACAAACCAGAAGAGAACAGACCACTAGGTTCTCAGCTCCCTAACTTCAA	5	0.125	No Hit
GCAAGACTTGAACTCGCCATGAAGTTTGGCTACGTCCTTCCTGATATTAC	5	0.125	No Hit
GGTAGGAAAAACAACCTCAGCGAAGATCCTGTACAACTATTTCAAAACAA	5	0.125	No Hit
TTTCAAGCAAAAATTAATATTGAAGTGCAGCTGGCTTCAGAATTAGCCAT	5	0.125	No Hit
AAATAATGTCATTTTTTATAATGGCTTCAATACATACACCTTGTAAAAGT	5	0.125	No Hit
GTGCAGTGTTAAAATGTTTTGCAAATACATACCAATGATACAGACTAAAT	5	0.125	No Hit
CTGTATTGAATATTATGATTGTTTTAAACGTGTTTAAAGATATTCTATAA	5	0.125	No Hit
GACATTCTTGAGTTTCTCTCTCCATTTCAGAAATAAGGTGTGTGCAGGCA	5	0.125	No Hit
CCCTGAGTCACTGGTGCCCTATTACACCGATGCAAAAAACCGGGGCTACC	5	0.125	No Hit
TAGCAACAAAGAAGCAAGGAATCTGTACACACCTGGGTCCCCATCCCTAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.025	0.0	0.0	0.0	0.0
82-83	0.025	0.0	0.0	0.0	0.0
84-85	0.075	0.0	0.0	0.0	0.0
86-87	0.1375	0.0	0.0	0.0	0.0
88-89	0.15	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAAAA	15	0.009267708	48.358974	88-89
>>END_MODULE
Read 5204763 spots for SRR32570290.sra
Written 5204763 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
Read 5204757 spots for SRR32570290.sra
Written 5204757 spots for SRR32570290.sra
SRR ids: ['SRR32570290.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_29csy1bl
SRR32570290.sra spots: 41638062
blocks: [[1, 5204757], [5204758, 10409514], [10409515, 15614271], [15614272, 20819028], [20819029, 26023785], [26023786, 31228542], [31228543, 36433299], [36433300, 41638062]]
SRR32570290 file size 10062532
SRR32570290 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32570290 SRR32570290_1.fastq SRR32570290_2.fastq
Input file:	SRR32570290_1.fastq
Paired file:	SRR32570290_2.fastq
trimmed:	SRR32570290-trimmed-pair1.fastq, SRR32570290-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 03:55:52 2026 >> started

Tue Mar 31 03:57:15 2026 >> done (82.798s)
41638062 read pairs processed; of these:
      74 ( 0.00%) short read pairs filtered out after trimming by size control
   78164 ( 0.19%) empty read pairs filtered out after trimming by size control
41559824 (99.81%) read pairs available; of these:
 1105359 ( 2.66%) trimmed read pairs available after processing
40454465 (97.34%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      15	  0.00%
 19	       3	  0.00%
 20	       6	  0.00%
 21	       4	  0.00%
 22	      12	  0.00%
 23	       6	  0.00%
 24	       5	  0.00%
 25	      10	  0.00%
 26	      17	  0.00%
 27	      19	  0.00%
 28	      12	  0.00%
 29	      16	  0.00%
 30	      32	  0.00%
 31	      35	  0.00%
 32	      50	  0.00%
 33	      45	  0.00%
 34	      58	  0.00%
 35	      51	  0.00%
 36	      76	  0.00%
 37	      85	  0.00%
 38	     112	  0.00%
 39	     124	  0.00%
 40	     145	  0.00%
 41	     178	  0.00%
 42	     157	  0.00%
 43	     200	  0.00%
 44	     170	  0.00%
 45	     223	  0.00%
 46	     288	  0.00%
 47	     298	  0.00%
 48	     363	  0.00%
 49	     430	  0.00%
 50	     527	  0.00%
 51	     591	  0.00%
 52	     626	  0.00%
 53	     673	  0.00%
 54	     739	  0.00%
 55	     826	  0.00%
 56	     881	  0.00%
 57	    1080	  0.00%
 58	    1208	  0.00%
 59	    1430	  0.00%
 60	    1714	  0.00%
 61	    1883	  0.00%
 62	    1981	  0.00%
 63	    2321	  0.01%
 64	    2459	  0.01%
 65	    2737	  0.01%
 66	    3020	  0.01%
 67	    3418	  0.01%
 68	    3833	  0.01%
 69	    4513	  0.01%
 70	    5226	  0.01%
 71	    5927	  0.01%
 72	    6840	  0.02%
 73	    7596	  0.02%
 74	    8184	  0.02%
 75	    9197	  0.02%
 76	   10110	  0.02%
 77	   11049	  0.03%
 78	   12364	  0.03%
 79	   13857	  0.03%
 80	   15747	  0.04%
 81	   17540	  0.04%
 82	   20013	  0.05%
 83	   22406	  0.05%
 84	   24727	  0.06%
 85	   27258	  0.07%
 86	   29354	  0.07%
 87	   31548	  0.08%
 88	   34346	  0.08%
 89	   37534	  0.09%
 90	   41704	  0.10%
 91	   46733	  0.11%
 92	   51488	  0.12%
 93	   55176	  0.13%
 94	   60694	  0.15%
 95	   64625	  0.16%
 96	   68626	  0.17%
 97	   72990	  0.18%
 98	   78094	  0.19%
 99	   83481	  0.20%
100	   91220	  0.22%
101	40454465	 97.34%
41559824 reads passed initial QC


criterion=sequence-density
sequence-density=1.40
sequence-density-rank=1
fanout-score=5.29
fanout-score-rank=19
prefix-density=7.35
prefix-fanout=1.0
sequence=GTCGTGGTATTACTGAAAGGCCC


criterion=fanout-score
sequence-density=0.05
sequence-density-rank=29
fanout-score=135.75
fanout-score-rank=1
prefix-density=7.43
prefix-fanout=1.0
sequence=GCGTCGTGGTAAT


criterion=sequence-density
sequence-density=2.51
sequence-density-rank=1
fanout-score=3.83
fanout-score-rank=22
prefix-density=4.78
prefix-fanout=2.0
sequence=AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA


criterion=fanout-score
sequence-density=0.08
sequence-density-rank=7
fanout-score=210.28
fanout-score-rank=1
prefix-density=9.81
prefix-fanout=1.7
sequence=GCCCACTTCGCGATCATATTCGT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GTCGTGGTATTACTGAAAGGCCC -y AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGACACAAACTAAAAAGCTCACTTGCCCACTTCCTTCCACAAGGAACTCCAATTTCACTAATTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTAGTAAGCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATCACATAGTTAATCCAA -o SRR32570290 SRR32570290_1.fastq SRR32570290_2.fastq
Input file:	SRR32570290_1.fastq
Paired file:	SRR32570290_2.fastq
trimmed:	SRR32570290-trimmed-pair1.fastq, SRR32570290-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GTCGTGGTATTACTGAAAGGCCC
-- paired 3' end adapter sequence (-y):	AATAATTGTTTCCCTAATCATATTTATTGGATCAACAAATCTCCTAGGCCTTTTACCACATACA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 31 03:59:00 2026 >> started

Tue Mar 31 03:59:19 2026 >> done (18.879s)
13853275 read pairs processed; of these:
     268 ( 0.00%) short read pairs filtered out after trimming by size control
    2523 ( 0.02%) empty read pairs filtered out after trimming by size control
13850484 (99.98%) read pairs available; of these:
     176 ( 0.00%) trimmed read pairs available after processing
13850308 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       3	  0.00%
 19	       3	  0.00%
 20	       2	  0.00%
 21	       1	  0.00%
 22	       5	  0.00%
 23	       2	  0.00%
 24	       2	  0.00%
 25	       2	  0.00%
 26	       8	  0.00%
 27	       8	  0.00%
 28	       3	  0.00%
 29	       5	  0.00%
 30	      10	  0.00%
 31	      17	  0.00%
 32	      17	  0.00%
 33	      21	  0.00%
 34	      18	  0.00%
 35	      19	  0.00%
 36	      28	  0.00%
 37	      33	  0.00%
 38	      35	  0.00%
 39	      40	  0.00%
 40	      46	  0.00%
 41	      69	  0.00%
 42	      58	  0.00%
 43	      60	  0.00%
 44	      43	  0.00%
 45	      71	  0.00%
 46	     101	  0.00%
 47	     106	  0.00%
 48	     106	  0.00%
 49	     121	  0.00%
 50	     176	  0.00%
 51	     203	  0.00%
 52	     223	  0.00%
 53	     219	  0.00%
 54	     258	  0.00%
 55	     248	  0.00%
 56	     301	  0.00%
 57	     343	  0.00%
 58	     365	  0.00%
 59	     470	  0.00%
 60	     587	  0.00%
 61	     633	  0.00%
 62	     659	  0.00%
 63	     804	  0.01%
 64	     793	  0.01%
 65	     907	  0.01%
 66	    1032	  0.01%
 67	    1139	  0.01%
 68	    1306	  0.01%
 69	    1475	  0.01%
 70	    1717	  0.01%
 71	    1940	  0.01%
 72	    2206	  0.02%
 73	    2542	  0.02%
 74	    2761	  0.02%
 75	    3002	  0.02%
 76	    3367	  0.02%
 77	    3609	  0.03%
 78	    3932	  0.03%
 79	    4583	  0.03%
 80	    5179	  0.04%
 81	    5830	  0.04%
 82	    6778	  0.05%
 83	    7282	  0.05%
 84	    8187	  0.06%
 85	    9068	  0.07%
 86	    9713	  0.07%
 87	   10562	  0.08%
 88	   11191	  0.08%
 89	   12427	  0.09%
 90	   13707	  0.10%
 91	   15363	  0.11%
 92	   17131	  0.12%
 93	   18258	  0.13%
 94	   19996	  0.14%
 95	   21406	  0.15%
 96	   22634	  0.16%
 97	   24150	  0.17%
 98	   26457	  0.19%
 99	   27761	  0.20%
100	   30940	  0.22%
101	13483601	 97.35%


criterion=sequence-density
sequence-density=0.31
sequence-density-rank=1
fanout-score=1.97
fanout-score-rank=38
prefix-density=0.30
prefix-fanout=2.0
sequence=GATAATAAATGG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=33
fanout-score=78.97
fanout-score-rank=1
prefix-density=0.24
prefix-fanout=6.8
sequence=TTTTTTAAATTAAATTATTGAAGCAGATCAGTTTTCGAAATATTTTAGTGGAACCATTTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAATACGGGGTTGTTGATTTCGTCTATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTTTCA


criterion=sequence-density
sequence-density=0.23
sequence-density-rank=1
fanout-score=2.38
fanout-score-rank=37
prefix-density=0.24
prefix-fanout=2.4
sequence=CCTAATCATATT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=33
fanout-score=41.14
fanout-score-rank=1
prefix-density=0.07
prefix-fanout=6.4
sequence=AGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTGAAATTGACCTTT
SRR32570290 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 31 04:00:24
                             Started mapping on |	Mar 31 04:00:25
                                    Finished on |	Mar 31 04:09:45
       Mapping speed, Million of reads per hour |	267.15

                          Number of input reads |	41557033
                      Average input read length |	201
                                    UNIQUE READS:
                   Uniquely mapped reads number |	29502765
                        Uniquely mapped reads % |	70.99%
                          Average mapped length |	200.59
                       Number of splices: Total |	13305095
            Number of splices: Annotated (sjdb) |	13135602
                       Number of splices: GT/AG |	13140294
                       Number of splices: GC/AG |	114284
                       Number of splices: AT/AC |	13559
               Number of splices: Non-canonical |	36958
                      Mismatch rate per base, % |	0.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.87
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.39
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2524043
             % of reads mapped to multiple loci |	6.07%
        Number of reads mapped to too many loci |	60193
             % of reads mapped to too many loci |	0.14%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	22.71%
                     % of reads unmapped: other |	0.08%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9530225	9530225	9530225
N_multimapping	2524043	2524043	2524043
N_noFeature	3125933	28439508	3321433
N_ambiguous	1235991	28604	373522
UnstrandedReadsAssigned:25140841 PositiveStrandReadsAssigned:1034653 NegativeStrandReadsAssigned:25807810
Dataset is classified negative stranded
MeadianReadLen=101 20thPercentileLength=101 echo kmer=97
SRR32570290 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32570290-trimmed-pair1.fastq
                             SRR32570290-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 41,557,033 reads, 30,579,418 reads pseudoaligned
[quant] estimated average fragment length: 186.784
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,500 rounds

 109283 SRR32570290.ke.tsv
  52636 SRR32570290.se.tsv
 161919 total
==> SRR32570290.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8	0	0
ENSMUST00000196221.1	9	8	0	0
ENSMUST00000179664.1	11	10	0	0
ENSMUST00000178537.1	12	11	0	0
ENSMUST00000178862.1	14	13	0	0
ENSMUST00000179520.1	11	10	0	0
ENSMUST00000179883.1	16	8	0	0
ENSMUST00000195858.1	10	9	0	0
ENSMUST00000179932.1	12	11	0	0

==> SRR32570290.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	244
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	2
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32570290 completed mapping pipeline successfully
