Starting /dee2/code/volunteer_pipeline.sh SRR32999362
    current disk space = 1071818224
    free memory = 199041140 
SRR32999362 SRAfilesize
ccec94eb0e80fa65b95df6271591e0fc  SRR32999362.sra
SRR32999362.sra file validated
SRR32999362 is paired end
SRR32999362 is conventional basespace
SRR32999362 read1 length is 248-251 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	248-251
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47725	33.0	32.0	34.0	32.0	34.0
2	32.81625	33.0	33.0	34.0	32.0	34.0
3	32.87575	33.0	33.0	34.0	32.0	34.0
4	33.00475	33.0	33.0	34.0	32.0	34.0
5	32.77175	33.0	32.0	34.0	32.0	34.0
6	35.2635	37.0	34.0	37.0	33.0	37.0
7	35.39575	37.0	34.0	37.0	33.0	37.0
8	35.08825	34.0	34.0	37.0	33.0	37.0
9	35.83925	37.0	34.0	37.0	33.0	37.0
10-14	36.953900000000004	37.4	37.4	37.4	36.6	37.4
15-19	37.70335	38.0	38.0	38.0	37.8	38.0
20-24	38.13955	38.4	38.4	38.4	38.4	38.4
25-29	38.4637	38.8	38.8	38.8	38.4	38.8
30-34	38.00255	38.4	38.4	38.4	37.8	39.0
35-39	38.5677	39.0	39.0	39.0	38.2	39.0
40-44	38.661699999999996	39.0	39.0	39.0	38.2	39.0
45-49	38.5073	39.0	38.8	39.0	38.2	39.0
50-54	38.46485	38.8	38.8	38.8	38.2	38.8
55-59	38.671	39.0	39.0	39.0	38.4	39.0
60-64	38.4538	39.0	39.0	39.0	37.8	39.0
65-69	38.78580000000001	39.0	39.0	39.0	39.0	39.0
70-74	38.418400000000005	38.8	38.8	38.8	38.0	39.0
75-79	38.74755	39.0	39.0	39.0	39.0	39.0
80-84	38.677699999999994	39.0	39.0	39.0	38.4	39.0
85-89	38.634	39.0	39.0	39.0	38.4	39.0
90-94	38.528499999999994	39.0	39.0	39.0	38.4	39.0
95-99	38.4259	39.0	39.0	39.0	37.8	39.0
100-104	38.2857	39.0	39.0	39.0	37.6	39.0
105-109	38.06055	39.0	38.4	39.0	37.4	39.0
110-114	38.08155	38.6	38.0	38.8	37.4	38.8
115-119	38.54495	39.0	39.0	39.0	38.0	39.0
120-124	38.31415	39.0	39.0	39.0	37.4	39.0
125-129	38.30105	39.0	38.8	39.0	37.6	39.0
130-134	37.9029	39.0	38.4	39.0	36.6	39.0
135-139	38.09075	39.0	38.0	39.0	37.4	39.0
140-144	38.43805	39.0	38.8	39.0	37.8	39.0
145-149	38.4962	38.8	38.8	38.8	38.6	38.8
150-154	38.102850000000004	39.0	38.6	39.0	37.2	39.0
155-159	38.36715	39.0	39.0	39.0	37.8	39.0
160-164	38.4894	39.0	39.0	39.0	38.0	39.0
165-169	38.4126	39.0	39.0	39.0	38.2	39.0
170-174	38.1244	39.0	38.8	39.0	37.6	39.0
175-179	38.17135	39.0	38.8	39.0	37.4	39.0
180-184	38.11345	39.0	38.8	39.0	37.4	39.0
185-189	38.1722	39.0	38.6	39.0	37.6	39.0
190-194	38.30775	39.0	39.0	39.0	37.6	39.0
195-199	38.215050000000005	39.0	38.8	39.0	37.4	39.0
200-204	37.48115	38.2	38.0	38.2	36.8	38.2
205-209	37.8409	38.6	38.6	38.6	37.6	38.6
210-214	38.02895	38.8	38.6	38.8	37.2	39.0
215-219	37.9821	39.0	38.6	39.0	37.2	39.0
220-224	37.816050000000004	39.0	38.2	39.0	36.8	39.0
225-229	37.99305	39.0	38.6	39.0	37.0	39.0
230-234	37.7013	39.0	38.0	39.0	36.4	39.0
235-239	37.810649999999995	39.0	38.0	39.0	37.2	39.0
240-244	36.9594	38.0	37.4	39.0	35.0	39.0
245-249	36.91733339584896	38.0	37.4	38.0	35.4	38.6
250-251	34.671743386168714	37.0	35.0	37.5	26.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-154	0.0
1101	155-159	0.0
1101	160-164	0.0
1101	165-169	0.0
1101	170-174	0.0
1101	175-179	0.0
1101	180-184	0.0
1101	185-189	0.0
1101	190-194	0.0
1101	195-199	0.0
1101	200-204	0.0
1101	205-209	0.0
1101	210-214	0.0
1101	215-219	0.0
1101	220-224	0.0
1101	225-229	0.0
1101	230-234	0.0
1101	235-239	0.0
1101	240-244	0.0
1101	245-249	0.0
1101	250-251	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	0.0
24	0.0
25	3.0
26	2.0
27	4.0
28	8.0
29	14.0
30	9.0
31	15.0
32	14.0
33	31.0
34	38.0
35	78.0
36	169.0
37	499.0
38	3113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.425000000000004	11.025	33.925	17.625
2	36.425000000000004	12.25	33.45	17.875
3	36.3	12.225	33.75	17.724999999999998
4	34.975	12.225	35.375	17.424999999999997
5	51.800000000000004	2.9000000000000004	0.375	44.925
6	3.375	96.35000000000001	0.125	0.15
7	97.425	0.42500000000000004	1.7999999999999998	0.35000000000000003
8	96.15	0.15	3.5749999999999997	0.125
9	1.125	0.15	97.375	1.35
10-14	24.57	21.415	11.075	42.94
15-19	31.16	21.27	25.915	21.654999999999998
20-24	21.755	20.085	21.349999999999998	36.809999999999995
25-29	75.885	3.35	18.535	2.23
30-34	39.475	20.474999999999998	20.105	19.945
35-39	22.215	16.7	45.355000000000004	15.73
40-44	38.735	37.385000000000005	2.465	21.415
45-49	42.43	18.85	19.775000000000002	18.945
50-54	18.72	1.815	20.485	58.98
55-59	39.475	39.45	20.25	0.8250000000000001
60-64	38.879999999999995	36.975	22.515	1.63
65-69	20.11	0.42	38.345	41.125
70-74	39.37	39.290000000000006	2.565	18.775
75-79	1.745	0.5950000000000001	59.03	38.629999999999995
80-84	38.36	19.794999999999998	20.495	21.349999999999998
85-89	0.19499999999999998	21.3	21.695	56.81
90-94	0.305	22.345000000000002	37.940000000000005	39.410000000000004
95-99	2.5149999999999997	38.080000000000005	21.099999999999998	38.305
100-104	1.775	21.029999999999998	39.535	37.66
105-109	38.845	38.78	20.18	2.1950000000000003
110-114	42.675000000000004	18.84	38.190000000000005	0.295
115-119	37.435	41.845	0.445	20.275000000000002
120-124	22.634999999999998	43.035000000000004	18.525	15.805
125-129	38.815	20.105	21.17	19.91
130-134	30.294999999999998	67.41	0.635	1.66
135-139	20.424999999999997	19.1	21.02	39.455
140-144	59.38	20.29	19.915	0.415
145-149	39.985	20.1	39.62	0.295
150-154	30.9	46.650000000000006	20.785	1.6650000000000003
155-159	1.0250000000000001	21.46	57.635000000000005	19.88
160-164	1.41	2.69	56.215	39.684999999999995
165-169	3.9050000000000002	56.910000000000004	21.325	17.86
170-174	39.415	38.355	21.740000000000002	0.49
175-179	1.2149999999999999	38.129999999999995	40.58	20.075000000000003
180-184	11.215	43.445	34.185	11.155
185-189	22.325	55.52	19.67	2.485
190-194	17.34	59.8	22.015	0.845
195-199	21.490000000000002	39.574999999999996	18.6	20.335
200-204	39.145	40.335	0.41000000000000003	20.11
205-209	37.88	20.4	3.595	38.125
210-214	18.965	21.78	39.300000000000004	19.955000000000002
215-219	20.325	20.18	36.205	23.29
220-224	22.919999999999998	74.79	0.73	1.5599999999999998
225-229	0.38	37.555	21.475	40.589999999999996
230-234	5.35	38.11	33.61	22.93
235-239	19.855	39.605000000000004	2.09	38.45
240-244	0.27499999999999997	19.96	39.085	40.68
245-249	39.58197909895495	21.6110805540277	36.72683634181709	2.08010400520026
250-251	38.64206827309237	7.216365461847389	4.354919678714859	49.78664658634538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	1.6666666666666665
44	47.66666666666673
45	324.999999999999
46	706.0000000000103
47	579.3333333333293
48	177.00000000000065
49	41.33333333333335
50	30.333333333333293
51	36.333333333333314
52	32.99999999999996
53	23.333333333333318
54	8.666666666666664
55	3.0
56	1.3333333333333333
57	0.3333333333333333
58	0.3333333333333333
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.3333333333333333
71	0.0
72	0.0
73	0.0
74	0.0
75	0.3333333333333333
76	0.3333333333333333
77	0.0
78	0.0
79	0.0
80	0.3333333333333333
81	0.3333333333333333
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
248	1.0
249	7.0
250	16.0
251	3976.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.52342487883683	65.4
2	9.24071082390953	14.299999999999999
3	3.4248788368336025	7.95
4	1.2924071082390953	4.0
5	0.7108239095315024	2.75
6	0.09693053311793215	0.44999999999999996
7	0.22617124394184168	1.225
8	0.03231017770597738	0.2
9	0.12924071082390953	0.8999999999999999
>10	0.32310177705977383	2.825
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	14	0.35000000000000003	No Hit
GGTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	13	0.325	No Hit
TGTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	13	0.325	No Hit
TTGGCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	11	0.27499999999999997	No Hit
TTGTCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	11	0.27499999999999997	No Hit
TTTCCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	11	0.27499999999999997	No Hit
GTTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	10	0.25	No Hit
GTGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	10	0.25	No Hit
GGGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	10	0.25	No Hit
TTGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	10	0.25	No Hit
GGGGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	9	0.22499999999999998	No Hit
GTTGGAGGTGCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	9	0.22499999999999998	No Hit
TTGGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	9	0.22499999999999998	No Hit
GGTTCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	9	0.22499999999999998	No Hit
CGTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	8	0.2	No Hit
TCTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
TGGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
AGTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
GGTACAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
GTGGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
GTGTGAGGTGCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
TGTGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	7	0.17500000000000002	No Hit
GTTACAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	6	0.15	No Hit
GGTGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	6	0.15	No Hit
AGGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	6	0.15	No Hit
GGGGCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGTTAGTGAAGCCTGGAG	5	0.125	No Hit
TGTTCAGGTTCAGCTGCAGCAGTCTGGGACTGAGCTTGTGATGCCTGGAG	5	0.125	No Hit
TGGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGTTAGTGAAGCCTGGAG	5	0.125	No Hit
TCGGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TGTTGAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TTTTCAGGTCCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
GGGGCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
GGGCCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TCGTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TGGGCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TTTTCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGTTAGTGAAGCCTGGAG	5	0.125	No Hit
TTCTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TGATCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
GTTTGAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
GGGGGAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TGTGCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TTTCGAGGTGCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
GCTTGAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
GTGGCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TTTTCAGGTTCAGCTGCAGCAGTCTGGGGCTGAGTTAGTGAAGCCTGGAG	5	0.125	No Hit
GTGTCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
TGGGCAGGTTCAGCTTCAGCAGTCTGGGGCTGAGCTAGTGAAGCCTGGAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.034999999999999996	0.0
60-64	0.0	0.0	0.0	0.075	0.0
65-69	0.0	0.0	0.0	0.075	0.0
70-74	0.0	0.0	0.0	0.075	0.0
75-79	0.0	0.0	0.0	0.12	0.0
80-84	0.0	0.0	0.0	0.125	0.0
85-89	0.0	0.0	0.0	0.125	0.0
90-94	0.0	0.0	0.0	0.125	0.0
95-99	0.0	0.0	0.0	0.125	0.0
100-104	0.0	0.0	0.0	0.125	0.0
105-109	0.0	0.0	0.0	0.125	0.0
110-114	0.0	0.0	0.0	0.125	0.0
115-119	0.0	0.0	0.0	0.125	0.0
120-124	0.0	0.0	0.0	0.16499999999999998	0.0
125-129	0.0	0.0	0.0	0.175	0.0
130-134	0.0	0.0	0.0	0.175	0.0
135-139	0.0	0.0	0.0	0.175	0.0
140-144	0.0	0.0	0.0	0.175	0.0
145-149	0.0	0.0	0.0	0.2	0.0
150-154	0.0	0.0	0.0	0.26	0.0
155-159	0.0	0.0	0.0	0.5	0.0
160-164	0.0	0.0	0.0	0.5	0.0
165-169	0.0	0.0	0.0	0.5	0.0
170-174	0.0	0.0	0.0	0.5	0.0
175-179	0.0	0.0	0.0	0.5	0.0
180-184	0.0	0.0	0.0	0.5	0.0
185-189	0.0	0.0	0.0	0.5	0.0
190-194	0.0	0.0	0.0	0.5	0.0
195-199	0.0	0.0	0.0	0.5	0.0
200-204	0.0	0.0	0.0	0.5	0.0
205-209	0.0	0.0	0.0	0.5	0.0
210-214	0.0	0.0	0.0	0.5	0.0
215-219	0.0	0.0	0.0	0.555	0.0
220-224	0.0	0.0	0.0	0.575	0.0
225-229	0.0	0.0	0.0	0.575	0.0
230-234	0.0	0.0	0.0	0.575	0.0
235-239	0.0	0.0	0.0	0.575	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAGG	10	0.0014218157	245.00002	2
GTAGAGG	10	0.0014218157	245.00002	2
GGAGAGG	10	0.0014218157	245.00002	2
TTGCAGG	10	0.0014218157	245.00002	2
GGGTCAG	10	0.0014218157	245.00002	1
CTGAGGT	10	0.0014218157	245.00002	3
CGGAGAG	10	0.0014218157	245.00002	1
GGTCCAA	10	0.0014218157	245.00002	7
GATGTGC	10	0.0014218157	245.00002	5
TGTTCAG	20	1.376684E-7	245.00002	1
TAAGAGG	10	0.0014218157	245.00002	2
GTTTCAG	10	0.0014218157	245.00002	1
GTTCAGG	20	1.376684E-7	245.00002	2
TTGAGGT	10	0.0014218157	245.00002	3
TGGAGGT	10	0.0014218157	245.00002	3
CGAGGTT	10	0.0014218157	245.00002	4
CGAGGTG	20	1.376684E-7	245.00002	4
ATGTGCA	10	0.0014218157	245.00002	6
AGGTGAA	10	0.0014218157	245.00002	6
ATCAGGT	10	0.0014218157	245.00002	3
>>END_MODULE
SRR32999362 read2 length is 248-251 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999362_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	248-251
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01625	32.0	32.0	33.0	29.0	33.0
2	31.5655	33.0	32.0	33.0	29.0	33.0
3	31.80625	33.0	32.0	33.0	30.0	34.0
4	31.74175	33.0	32.0	33.0	31.0	34.0
5	32.361	33.0	32.0	33.0	32.0	34.0
6	33.77475	35.0	33.0	37.0	31.0	37.0
7	34.50625	35.0	33.0	37.0	32.0	37.0
8	34.5975	35.0	33.0	37.0	32.0	37.0
9	35.32075	37.0	33.0	37.0	32.0	37.0
10-14	35.2632	35.8	35.4	36.2	34.4	36.4
15-19	36.5818	38.0	36.6	38.0	35.4	38.0
20-24	37.097950000000004	38.2	37.8	38.4	36.0	38.4
25-29	37.73345	38.8	38.2	38.8	36.6	38.8
30-34	37.56	38.8	38.2	38.8	36.4	38.8
35-39	37.1645	38.0	37.6	38.4	35.6	38.6
40-44	38.13525	39.0	38.8	39.0	37.4	39.0
45-49	37.25599999999999	38.2	38.2	38.2	36.4	38.2
50-54	37.892	38.8	38.6	38.8	37.4	38.8
55-59	37.56205	38.8	38.2	38.8	36.6	38.8
60-64	37.0514	38.0	38.0	38.2	36.2	38.2
65-69	37.1774	38.4	38.0	38.4	35.6	39.0
70-74	37.60485	38.8	38.2	38.8	36.4	39.0
75-79	37.91075	39.0	38.6	39.0	37.4	39.0
80-84	37.7329	39.0	38.2	39.0	37.0	39.0
85-89	37.21375	38.4	38.0	38.4	36.6	38.4
90-94	37.91485	39.0	38.8	39.0	37.2	39.0
95-99	36.9793	38.8	38.0	38.8	34.8	38.8
100-104	36.31595	38.0	37.6	38.0	33.6	38.2
105-109	36.8452	38.6	37.8	38.6	34.6	39.0
110-114	37.34285	39.0	38.0	39.0	35.8	39.0
115-119	37.1291	38.8	38.0	38.8	35.2	39.0
120-124	37.249249999999996	39.0	38.0	39.0	35.2	39.0
125-129	37.60815	39.0	38.4	39.0	36.8	39.0
130-134	37.162349999999996	39.0	38.0	39.0	35.2	39.0
135-139	37.039249999999996	39.0	38.0	39.0	34.4	39.0
140-144	37.3767	39.0	38.4	39.0	36.2	39.0
145-149	37.03005	39.0	38.0	39.0	34.8	39.0
150-154	37.1333	39.0	38.0	39.0	35.4	39.0
155-159	36.0457	38.0	37.6	38.0	32.8	38.2
160-164	36.77455	39.0	38.0	39.0	33.8	39.0
165-169	36.92045	39.0	38.0	39.0	34.0	39.0
170-174	36.888250000000006	39.0	38.0	39.0	34.0	39.0
175-179	36.96055	39.0	38.0	39.0	32.8	39.0
180-184	36.8535	39.0	37.8	39.0	33.8	39.0
185-189	37.06895	39.0	37.8	39.0	35.2	39.0
190-194	36.69785	38.2	37.8	39.0	34.0	39.0
195-199	36.20005	38.0	37.2	38.6	33.0	39.0
200-204	35.77735	38.0	37.0	38.0	30.6	38.4
205-209	35.826899999999995	38.0	37.0	38.0	32.2	38.0
210-214	35.8108	38.0	37.0	38.0	31.6	38.0
215-219	35.47255	38.0	37.0	38.0	29.8	38.0
220-224	36.185900000000004	38.0	37.0	38.0	34.0	38.0
225-229	35.754599999999996	37.8	37.0	38.0	33.0	38.0
230-234	35.725500000000004	37.0	37.0	38.0	33.0	38.0
235-239	34.78915	37.0	36.4	37.0	26.6	38.0
240-244	35.110800000000005	37.0	37.0	37.0	31.6	37.4
245-249	34.7607691622774	37.0	37.0	37.0	27.2	37.0
250-251	33.91922517379512	37.0	35.0	37.0	24.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-154	0.0
1101	155-159	0.0
1101	160-164	0.0
1101	165-169	0.0
1101	170-174	0.0
1101	175-179	0.0
1101	180-184	0.0
1101	185-189	0.0
1101	190-194	0.0
1101	195-199	0.0
1101	200-204	0.0
1101	205-209	0.0
1101	210-214	0.0
1101	215-219	0.0
1101	220-224	0.0
1101	225-229	0.0
1101	230-234	0.0
1101	235-239	0.0
1101	240-244	0.0
1101	245-249	0.0
1101	250-251	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	11.0
17	25.0
18	5.0
19	4.0
20	5.0
21	12.0
22	8.0
23	7.0
24	13.0
25	14.0
26	16.0
27	19.0
28	33.0
29	38.0
30	37.0
31	57.0
32	59.0
33	81.0
34	100.0
35	154.0
36	306.0
37	1352.0
38	1643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.425	19.175	29.65	20.75
2	27.05	20.225	30.2	22.525000000000002
3	28.475	19.425	29.225	22.875
4	29.925	18.8	28.4	22.875
5	97.55	1.325	0.9249999999999999	0.2
6	2.675	95.8	0.125	1.4000000000000001
7	96.975	1.15	0.35000000000000003	1.525
8	1.575	0.475	1.7000000000000002	96.25
9	0.8750000000000001	0.05	95.6	3.4750000000000005
10-14	59.605	0.22499999999999998	20.52	19.650000000000002
15-19	59.52499999999999	19.64	20.535	0.3
20-24	22.7	19.615	37.585	20.1
25-29	59.97	18.54	21.425	0.065
30-34	57.52	21.285	21.075	0.12
35-39	61.135	18.645	20.044999999999998	0.17500000000000002
40-44	0.44999999999999996	1.7399999999999998	77.09	20.72
45-49	38.355	20.64	1.79	39.215
50-54	2.775	19.384999999999998	38.06	39.78
55-59	58.01	37.964999999999996	1.92	2.105
60-64	55.515	21.57	3.02	19.895
65-69	24.595	21.435000000000002	34.9	19.07
70-74	58.489999999999995	18.045	22.830000000000002	0.635
75-79	3.995	0.35500000000000004	55.17999999999999	40.47
80-84	21.505	17.025000000000002	2.945	58.525000000000006
85-89	20.855	38.165	19.275000000000002	21.705
90-94	19.285	22.81	20.595	37.31
95-99	38.495000000000005	24.01	18.195	19.3
100-104	36.309999999999995	21.21	20.035	22.445
105-109	32.379999999999995	23.724999999999998	20.810000000000002	23.085
110-114	1.79	19.18	21.77	57.26
115-119	19.415	2.93	39.285	38.37
120-124	15.215	46.56	16.634999999999998	21.59
125-129	5.035	70.695	21.685	2.585
130-134	19.52	38.345	2.215	39.92
135-139	25.345000000000002	38.145	19.13	17.380000000000003
140-144	3.64	37.059999999999995	21.445	37.855
145-149	35.03	24.525	36.144999999999996	4.3
150-154	3.5249999999999995	37.89	38.879999999999995	19.705000000000002
155-159	53.935	3.46	20.825	21.78
160-164	22.325	19.295	23.095	35.285
165-169	20.974999999999998	38.75	19.045	21.23
170-174	22.439999999999998	37.875	20.52	19.165
175-179	38.035000000000004	2.56	53.27499999999999	6.13
180-184	56.68	34.589999999999996	7.105	1.625
185-189	4.24	18.67	75.445	1.645
190-194	4.87	18.95	37.305	38.875
195-199	21.705	36.485	20.815	20.995
200-204	37.63	20.265	39.5	2.605
205-209	19.455	4.89	37.565	38.09
210-214	19.85	1.82	22.375	55.955
215-219	20.674999999999997	37.685	25.264999999999997	16.375
220-224	1.67	2.45	73.055	22.825
225-229	6.6000000000000005	33.650000000000006	56.815000000000005	2.935
230-234	6.215	36.88	44.07	12.834999999999999
235-239	18.145	24.735	38.06	19.06
240-244	2.315	38.23	38.51	20.945
245-249	19.102416570771002	38.76519737829589	39.840896582778804	2.2914894681543005
250-251	91.27535426507363	1.0002778549597109	3.6676854681856073	4.0566824117810505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	1.0
46	1.6666666666666665
47	18.333333333333336
48	124.9999999999993
49	339.9999999999981
50	688.666666666675
51	561.3333333333272
52	129.66666666666598
53	49.33333333333341
54	49.00000000000007
55	24.333333333333314
56	15.333333333333343
57	8.33333333333333
58	4.999999999999999
59	1.3333333333333333
60	0.6666666666666666
61	0.6666666666666666
62	0.6666666666666666
63	0.6666666666666666
64	0.6666666666666666
65	0.3333333333333333
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
248	13.0
249	274.0
250	228.0
251	3485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.074999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28913260219342	17.625
2	3.788634097706879	1.9
3	1.0967098703888334	0.8250000000000001
4	0.7976071784646062	0.8
5	1.0967098703888334	1.375
6	1.5952143569292123	2.4
7	1.4955134596211366	2.625
8	1.5952143569292123	3.2
9	1.5952143569292123	3.5999999999999996
>10	16.650049850448653	65.64999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	31	0.775	No Hit
GTTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	30	0.75	No Hit
TGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	30	0.75	No Hit
GGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	28	0.7000000000000001	No Hit
TTGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	28	0.7000000000000001	No Hit
TATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	27	0.675	No Hit
GTCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	27	0.675	No Hit
TTGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	27	0.675	No Hit
GTGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
TTCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
GTGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
GTGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	25	0.625	No Hit
TTTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	25	0.625	No Hit
GCGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
TTTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
GCAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
TATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
GGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
TGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
GTTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
GTTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TTTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
GTATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	21	0.525	No Hit
GGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	21	0.525	No Hit
TGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TTCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
CTTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GCTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
CTGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
AAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
TGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
TCGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
TTGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
AGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
TCCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
TTCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
GGTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
TTCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
CTCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
GAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
TCTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
GGCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
CGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
CGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
TTTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
ATTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
ATTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
GCTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
TCATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
CGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
ACTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
CCCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TTATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
GTCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
CTGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TTAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
GATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
GCGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
GCTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
GCTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
CGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TGCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CCGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CCTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
ACCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
ATCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CTTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CTTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GTAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
ATGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
AGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
TAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TTGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TCTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TTACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
ATGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
GCATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CTATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TCGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
GCCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CGTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TCCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CCGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TCAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
GCGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
AGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TCTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GCCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ATTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ATGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ATGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TACTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ATCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CTCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ACTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
AATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
AACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
ACGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
AGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
GTCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CCCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
GCAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
AATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CTCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
ACTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TCAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
GGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TCGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
ACGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GGAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
ACCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CCAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
TAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
TCCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CCGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
AATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
TGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
AAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
TTAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GTCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
ATAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GTTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
TCACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GTACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
AGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
TATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GCCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
ATCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CCAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
TATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CGCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GCGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CCTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GTAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
ACGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CTACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GAGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CTAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
TAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GCACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CCATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
TAGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CCTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CCCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GCCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CTAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AGTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GACTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ACCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CTGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ATAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CTGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ATCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CCGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ATACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
AAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ATATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
GAACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
TCCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
AACTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
TGAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
ACGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
CGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
TACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
ACACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
ACCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
CTTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
CCCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
GAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
AGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
GAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
AAACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
AGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
AACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
TCGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
GACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
TCTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
GTGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
ACATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
AGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
CGAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
AAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
ATTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
AGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
CCTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
TTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC	5	0.125	No Hit
CAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.034999999999999996	0.0
60-64	0.0	0.0	0.0	0.075	0.0
65-69	0.0	0.0	0.0	0.075	0.0
70-74	0.0	0.0	0.0	0.075	0.0
75-79	0.0	0.0	0.0	0.12	0.0
80-84	0.0	0.0	0.0	0.125	0.0
85-89	0.0	0.0	0.0	0.125	0.0
90-94	0.0	0.0	0.0	0.125	0.0
95-99	0.0	0.0	0.0	0.125	0.0
100-104	0.0	0.0	0.0	0.125	0.0
105-109	0.0	0.0	0.0	0.125	0.0
110-114	0.0	0.0	0.0	0.125	0.0
115-119	0.0	0.0	0.0	0.125	0.0
120-124	0.0	0.0	0.0	0.16499999999999998	0.0
125-129	0.0	0.0	0.0	0.175	0.0
130-134	0.0	0.0	0.0	0.175	0.0
135-139	0.0	0.0	0.0	0.175	0.0
140-144	0.0	0.0	0.0	0.175	0.0
145-149	0.0	0.0	0.0	0.2	0.0
150-154	0.0	0.0	0.0	0.25	0.0
155-159	0.0	0.0	0.0	0.45	0.0
160-164	0.0	0.0	0.0	0.45	0.0
165-169	0.0	0.0	0.0	0.45	0.0
170-174	0.0	0.0	0.0	0.45	0.0
175-179	0.0	0.0	0.0	0.45	0.0
180-184	0.0	0.0	0.0	0.45	0.0
185-189	0.0	0.0	0.0	0.45	0.0
190-194	0.0	0.0	0.0	0.45	0.0
195-199	0.0	0.0	0.0	0.45	0.0
200-204	0.0	0.0	0.0	0.45	0.0
205-209	0.0	0.0	0.0	0.45	0.0
210-214	0.0	0.0	0.0	0.45	0.0
215-219	0.0	0.0	0.0	0.505	0.0
220-224	0.0	0.0	0.0	0.525	0.0
225-229	0.0	0.0	0.0	0.525	0.0
230-234	0.0	0.0	0.0	0.525	0.0
235-239	0.0	0.0	0.0	0.525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAAGG	15	8.689032E-6	275.87326	245
TGTAAGG	340	0.0	255.58844	245
GGAGAGC	15	1.4127998E-5	244.83751	2
CCGGAGC	15	1.4127998E-5	244.83751	2
CGGAGCT	50	0.0	244.83751	3
CCTGAGC	15	1.4127998E-5	244.83751	2
GAGCTCG	380	0.0	244.83751	5
TCGGAGC	15	1.4127998E-5	244.83751	2
TGGAGCT	30	1.2732926E-11	244.83751	3
AGGGAGC	15	1.4127998E-5	244.83751	2
GTGAGCT	50	0.0	244.83751	3
TAGAGCT	15	1.4127998E-5	244.83751	3
GAGAGCT	15	1.4127998E-5	244.83751	3
CTTGAGC	15	1.4127998E-5	244.83751	2
TTGGAGC	15	1.4127998E-5	244.83751	2
GGAGCTC	120	0.0	244.83751	4
ACGGAGC	15	1.4127998E-5	244.83751	2
GCGAGCT	15	1.4127998E-5	244.83751	3
TTTGAGC	10	0.001424643	244.8375	2
TGTGAGC	10	0.001424643	244.8375	2
>>END_MODULE
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
Read 66184 spots for SRR32999362.sra
Written 66184 spots for SRR32999362.sra
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
Read 66178 spots for SRR32999362.sra
Written 66178 spots for SRR32999362.sra
SRR ids: ['SRR32999362.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bp8vs635
SRR32999362.sra spots: 529430
blocks: [[1, 66178], [66179, 132356], [132357, 198534], [198535, 264712], [264713, 330890], [330891, 397068], [397069, 463246], [463247, 529430]]
SRR32999362 file size 301056
SRR32999362 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32999362 SRR32999362_1.fastq SRR32999362_2.fastq
Input file:	SRR32999362_1.fastq
Paired file:	SRR32999362_2.fastq
trimmed:	SRR32999362-trimmed-pair1.fastq, SRR32999362-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 17 17:55:36 2026 >> started

Tue Mar 17 17:55:37 2026 >> done (0.713s)
529430 read pairs processed; of these:
     0 ( 0.00%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
529430 (100.00%) read pairs available; of these:
529430 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
245	     1	  0.00%
246	     0	  0.00%
247	     1	  0.00%
248	    25	  0.00%
249	  1188	  0.22%
250	 63486	 11.99%
251	464729	 87.78%
529430 reads passed initial QC


criterion=sequence-density
sequence-density=7.18
sequence-density-rank=1
fanout-score=34.85
fanout-score-rank=3
prefix-density=7.57
prefix-fanout=33.0
sequence=GAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGACTCTGAGGAGACGGTGACTGAGGTTCCTTGACCCCAGTAGTCCATAGCGGCGTAGTGACTACCCCTTGCACAAAAATAAACGGCAGACTCATCAGATGTTAATCGGCTGAGCTCCAAGTAGACTGTGTTAGAGGATTTTTCTACAGTCAATGTGGCCTTGCCCTTGAACTTTTCATTATACTTAGTATCATCATTGTAAGGAT


criterion=fanout-score
sequence-density=0.32
sequence-density-rank=15
fanout-score=793.03
fanout-score-rank=1
prefix-density=7.57
prefix-fanout=33.0
sequence=GAGCTCGTGGGGAGTGTCAG
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x -y GAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGACTCTGAGGAGACGGTGACTGAGGTTCCTTGACCCCAGTAGTCCATAGCGGCGTAGTGACTACCCCTTGCACAAAAATAAACGGCAGACTCATCAGATGTTAATCGGCTGAGCTCCAAGTAGACTGTGTTAGAGGATTTTTCTACAGTCAATGTGGCCTTGCCCTTGAACTTTTCATTATACTTAGTATCATCATTGTAAGGAT -o SRR32999362 SRR32999362_1.fastq SRR32999362_2.fastq
Input file:	GAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGACTCTGAGGAGACGGTGACTGAGGTTCCTTGACCCCAGTAGTCCATAGCGGCGTAGTGACTACCCCTTGCACAAAAATAAACGGCAGACTCATCAGATGTTAATCGGCTGAGCTCCAAGTAGACTGTGTTAGAGGATTTTTCTACAGTCAATGTGGCCTTGCCCTTGAACTTTTCATTATACTTAGTATCATCATTGTAAGGAT
Paired file:	SRR32999362_1.fastq
trimmed:	SRR32999362-trimmed-pair1.fastq, SRR32999362-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	-y
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 17 17:55:40 2026 >> started


criterion=sequence-density
sequence-density=7.20
sequence-density-rank=1
fanout-score=30.48
fanout-score-rank=3
prefix-density=7.25
prefix-fanout=30.3
sequence=GAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGACTCTGAGGAGACGGTGACTGAGGTTCCTTGACCCCAGTAGTCCATAGCGGCGTAGTGACTACCCCTTGCACAAAAATAAACGGCAGACTCATCAGATGTTAATCGGCTGAGCTCCAAGTAGACTGTGTTAGAGGATTTTTCTACAGTCAATGTGGCCTTGCCCTTGAACTTTTCATTATACTTAGTATCATCATTGTAAGG


criterion=fanout-score
sequence-density=0.32
sequence-density-rank=15
fanout-score=695.39
fanout-score-rank=1
prefix-density=7.25
prefix-fanout=30.3
sequence=GAGCTCGTGGGGAGTGTCAG
SRR32999362 testing PE reads STAR mapping to Ensembl genome
Read2 map rate below 40%, dropping it and using Read1 only
                                 Started job on |	Mar 17 17:56:40
                             Started mapping on |	Mar 17 17:56:40
                                    Finished on |	Mar 17 17:56:44
       Mapping speed, Million of reads per hour |	238.24

                          Number of input reads |	264715
                      Average input read length |	230
                                    UNIQUE READS:
                   Uniquely mapped reads number |	259337
                        Uniquely mapped reads % |	97.97%
                          Average mapped length |	230.41
                       Number of splices: Total |	347
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	241
                       Number of splices: GC/AG |	83
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	23
                      Mismatch rate per base, % |	2.59%
                         Deletion rate per base |	0.01%
                        Deletion average length |	3.49
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1177
             % of reads mapped to multiple loci |	0.44%
        Number of reads mapped to too many loci |	4
             % of reads mapped to too many loci |	0.00%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.99%
                     % of reads unmapped: other |	0.60%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4201	4201	4201
N_multimapping	1177	1177	1177
N_noFeature	0	0	259334
N_ambiguous	340	340	0
UnstrandedReadsAssigned:258997 PositiveStrandReadsAssigned:258997 NegativeStrandReadsAssigned:3
Dataset is classified positive stranded
MeadianReadLen=231 20thPercentileLength=231 echo kmer=227
SRR32999362 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR32999362-trimmed-pair1.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 264,715 reads, 217,396 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,115 rounds

 109283 SRR32999362.ke.tsv
  52636 SRR32999362.se.tsv
 161919 total
==> SRR32999362.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR32999362.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32999362 completed mapping pipeline successfully
