Starting /dee2/code/volunteer_pipeline.sh SRR32999411
    current disk space = 1061254728
    free memory = 197812920 
SRR32999411 SRAfilesize
138e3fb9fed82dea5dec7c130e1ab06e  SRR32999411.sra
SRR32999411.sra file validated
SRR32999411 is paired end
SRR32999411 is conventional basespace
SRR32999411 read1 length is 248-251 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	248-251
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83125	33.0	32.0	34.0	32.0	34.0
2	32.97525	33.0	33.0	34.0	32.0	34.0
3	32.872	33.0	33.0	34.0	32.0	34.0
4	33.00775	33.0	33.0	34.0	32.0	34.0
5	32.86825	33.0	33.0	34.0	32.0	34.0
6	35.40625	37.0	34.0	37.0	33.0	37.0
7	35.55	37.0	34.0	37.0	33.0	37.0
8	35.05425	35.0	33.0	37.0	33.0	37.0
9	35.71775	37.0	34.0	37.0	32.0	37.0
10-14	36.51225	37.4	36.6	37.4	35.8	37.4
15-19	37.14505	38.0	37.8	38.0	36.0	38.0
20-24	37.7765	38.4	38.2	38.4	37.2	38.4
25-29	38.473850000000006	39.0	38.6	39.0	37.8	39.0
30-34	38.26755000000001	39.0	38.0	39.0	37.6	39.0
35-39	38.5972	39.0	38.8	39.0	38.2	39.0
40-44	38.47605	39.0	38.8	39.0	37.8	39.0
45-49	38.0678	39.0	38.4	39.0	37.2	39.0
50-54	37.89805	38.0	38.0	38.2	37.6	38.8
55-59	38.628750000000004	39.0	39.0	39.0	38.2	39.0
60-64	38.07995	39.0	38.6	39.0	34.4	39.0
65-69	38.609700000000004	39.0	39.0	39.0	38.2	39.0
70-74	38.327200000000005	38.8	38.6	39.0	38.0	39.0
75-79	38.74185	39.0	39.0	39.0	38.8	39.0
80-84	38.57865	39.0	38.8	39.0	38.0	39.0
85-89	38.23965	39.0	38.6	39.0	37.6	39.0
90-94	38.43845	39.0	38.8	39.0	38.0	39.0
95-99	38.38845	39.0	38.6	39.0	37.4	39.0
100-104	38.30630000000001	39.0	38.2	39.0	37.6	39.0
105-109	38.6392	39.0	39.0	39.0	38.4	39.0
110-114	38.78895	39.0	39.0	39.0	38.6	39.0
115-119	38.20095	38.8	38.0	38.8	37.8	39.0
120-124	38.40775	39.0	38.4	39.0	38.0	39.0
125-129	38.1705	38.6	38.4	39.0	37.6	39.0
130-134	38.49305	39.0	38.8	39.0	38.0	39.0
135-139	38.3605	39.0	38.0	39.0	38.0	39.0
140-144	38.51485	39.0	38.6	39.0	38.0	39.0
145-149	38.4427	38.8	38.8	38.8	37.8	39.0
150-154	38.26905	39.0	38.4	39.0	38.0	39.0
155-159	38.4565	39.0	38.8	39.0	37.8	39.0
160-164	38.5022	39.0	39.0	39.0	38.0	39.0
165-169	38.23625	39.0	38.2	39.0	37.6	39.0
170-174	38.2927	39.0	38.6	39.0	37.6	39.0
175-179	38.29995	39.0	39.0	39.0	37.6	39.0
180-184	38.3008	39.0	38.8	39.0	37.6	39.0
185-189	38.3135	38.8	38.6	39.0	37.6	39.0
190-194	38.06755	39.0	38.0	39.0	37.0	39.0
195-199	38.1443	39.0	38.2	39.0	37.2	39.0
200-204	37.668099999999995	38.2	38.0	38.2	36.8	39.0
205-209	37.65505	38.6	38.0	38.6	36.6	39.0
210-214	37.92575	38.8	38.0	38.8	37.0	39.0
215-219	37.914049999999996	39.0	38.0	39.0	37.0	39.0
220-224	37.6992	38.0	38.0	39.0	37.0	39.0
225-229	37.6284	38.0	37.6	39.0	36.6	39.0
230-234	37.798899999999996	38.0	38.0	39.0	37.0	39.0
235-239	37.35115	38.0	37.8	38.4	36.8	38.8
240-244	37.00455000000001	38.0	37.0	38.0	36.6	38.0
245-249	37.018200062515625	38.0	37.0	38.0	37.0	38.0
250-251	33.950050231181315	35.5	31.5	37.5	26.0	37.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1102	1	0.0
1102	2	0.0
1102	3	0.0
1102	4	0.0
1102	5	0.0
1102	6	0.0
1102	7	0.0
1102	8	0.0
1102	9	0.0
1102	10-14	0.0
1102	15-19	0.0
1102	20-24	0.0
1102	25-29	0.0
1102	30-34	0.0
1102	35-39	0.0
1102	40-44	0.0
1102	45-49	0.0
1102	50-54	0.0
1102	55-59	0.0
1102	60-64	0.0
1102	65-69	0.0
1102	70-74	0.0
1102	75-79	0.0
1102	80-84	0.0
1102	85-89	0.0
1102	90-94	0.0
1102	95-99	0.0
1102	100-104	0.0
1102	105-109	0.0
1102	110-114	0.0
1102	115-119	0.0
1102	120-124	0.0
1102	125-129	0.0
1102	130-134	0.0
1102	135-139	0.0
1102	140-144	0.0
1102	145-149	0.0
1102	150-154	0.0
1102	155-159	0.0
1102	160-164	0.0
1102	165-169	0.0
1102	170-174	0.0
1102	175-179	0.0
1102	180-184	0.0
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-251	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	2.0
25	1.0
26	1.0
27	5.0
28	3.0
29	6.0
30	11.0
31	7.0
32	12.0
33	22.0
34	37.0
35	69.0
36	168.0
37	778.0
38	2876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.525	10.2	38.625	16.650000000000002
2	33.550000000000004	10.525	38.625	17.299999999999997
3	33.525	11.600000000000001	37.8	17.075000000000003
4	32.9	11.1	39.050000000000004	16.950000000000003
5	47.075	1.9	0.125	50.9
6	2.7	96.975	0.17500000000000002	0.15
7	96.5	0.6	2.5250000000000004	0.375
8	96.575	0.3	2.9749999999999996	0.15
9	1.225	0.2	97.475	1.0999999999999999
10-14	26.919999999999998	23.22	8.129999999999999	41.730000000000004
15-19	38.725	19.145	28.505000000000003	13.625000000000002
20-24	25.540000000000003	20.25	20.669999999999998	33.54
25-29	50.42	13.05	24.055	12.475
30-34	41.855	26.755000000000003	14.075	17.315
35-39	37.364999999999995	1.06	45.905	15.67
40-44	40.815	34.945	0.215	24.025
45-49	40.589999999999996	11.62	17.035	30.755
50-54	25.77	1.755	33.015	39.46
55-59	33.725	39.0	22.765	4.51
60-64	21.42	43.004999999999995	24.13	11.445
65-69	19.98	2.7449999999999997	38.93	38.345
70-74	37.145	36.19	3.605	23.06
75-79	1.695	0.215	55.779999999999994	42.309999999999995
80-84	38.66	17.235	37.475	6.63
85-89	2.85	25.905	30.435000000000002	40.81
90-94	1.77	28.945	37.885000000000005	31.4
95-99	14.31	42.035	17.555	26.1
100-104	8.04	46.585	10.51	34.865
105-109	19.505	34.32	20.810000000000002	25.365
110-114	60.62499999999999	0.395	38.605000000000004	0.375
115-119	34.665	34.75	1.37	29.215000000000003
120-124	44.125	36.449999999999996	3.75	15.675
125-129	36.144999999999996	30.0	17.05	16.805
130-134	29.37	64.64999999999999	0.485	5.495
135-139	27.555000000000003	0.43	39.025	32.99
140-144	56.42	23.43	19.975	0.17500000000000002
145-149	43.295	16.485	39.58	0.64
150-154	37.15	35.545	16.950000000000003	10.355
155-159	6.890000000000001	53.480000000000004	39.115	0.515
160-164	8.08	22.765	35.68	33.475
165-169	11.76	40.915	31.730000000000004	15.595
170-174	36.33	29.93	21.884999999999998	11.855
175-179	3.82	36.85	41.085	18.245
180-184	11.765	34.55	21.005	32.68
185-189	7.115	46.94	21.925	24.02
190-194	37.475	34.65	20.599999999999998	7.2749999999999995
195-199	19.32	36.94	21.709999999999997	22.03
200-204	38.85	40.37	2.895	17.885
205-209	34.544999999999995	26.229999999999997	1.7999999999999998	37.425000000000004
210-214	16.43	23.715	37.714999999999996	22.14
215-219	17.47	22.915	31.96	27.655
220-224	17.625	42.76	3.05	36.565
225-229	6.4350000000000005	35.275	31.905	26.384999999999998
230-234	16.91	25.45	20.919999999999998	36.720000000000006
235-239	19.400000000000002	44.269999999999996	0.455	35.875
240-244	6.285	20.345	22.12	51.24999999999999
245-249	18.96594829741487	32.82164108205411	22.856142807140355	25.35626781339067
250-251	38.467442752974286	2.8271715491876677	10.093386209543304	48.61199948829474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.6666666666666666
45	14.333333333333341
46	219.3333333333352
47	602.9999999999986
48	440.9999999999924
49	137.6666666666662
50	76.66666666666666
51	125.99999999999929
52	182.0000000000008
53	121.33333333333269
54	50.66666666666675
55	19.0
56	4.999999999999999
57	1.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
248	1.0
249	7.0
250	167.0
251	3825.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.40280880944782	67.675
2	8.33067347590169	13.05
3	2.2661985317586977	5.325
4	1.212894988828599	3.8
5	0.7022023619533994	2.75
6	0.4468560485157995	2.1
7	0.1915097350781998	1.05
8	0.12767315671879986	0.8
9	0.031918289179699966	0.22499999999999998
>10	0.28726460261729975	3.225
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	24	0.6	No Hit
TGGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	16	0.4	No Hit
TGTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	15	0.375	No Hit
GGTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	14	0.35000000000000003	No Hit
TTGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	13	0.325	No Hit
GTGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	13	0.325	No Hit
GTTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	13	0.325	No Hit
GGGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	11	0.27499999999999997	No Hit
TTTGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	10	0.25	No Hit
TTTTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	9	0.22499999999999998	No Hit
TTGGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	8	0.2	No Hit
TGCTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	8	0.2	No Hit
GTGGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	8	0.2	No Hit
TCTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	8	0.2	No Hit
TTGTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	7	0.17500000000000002	No Hit
GTTTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	7	0.17500000000000002	No Hit
TATTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	7	0.17500000000000002	No Hit
TGGGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	7	0.17500000000000002	No Hit
GGTGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	7	0.17500000000000002	No Hit
CTTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	7	0.17500000000000002	No Hit
TTGCCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TCGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TCTCCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
GTTTCAGGTTCAACTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TTTTGAGGTGCAGCTTGTTGAGTCTGGTGGAGGATTGGTGCAGCCTAAAG	6	0.15	No Hit
GCGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
CGTTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
GTGTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TATGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TGTTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
GGTTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TTCTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
ATGGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TGTGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	6	0.15	No Hit
TTGGCAGGTCCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GGGGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GGGGCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GTTGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GTGTCAGGTTCAGCTTCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GTGGGAGGTCCAGCTGCAACAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TTTGGAGGTGCAGGTGGTGGAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TGGCCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
CAGTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TCTTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TGGTCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
CATTCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TGGGCAGGTCCACGTGAAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TTGGGAGGTGCAGCTTGTTGAGTCTGGTGGAGGATTGGTGCAGCCTAAAG	5	0.125	No Hit
GCGGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TTGTCAGGTCCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TGGTGAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TGTTCAGGTTCAGCTTCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GTTTGAGGTGCAGCTTGTTGAGTCTGGTGGAGGATTGGTGCAGCCTAAAG	5	0.125	No Hit
GTCGCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
TTTTCAGGTTCAACTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
GTGCCAGGTTCAGCTGCAGCAGTCTGGACCTGAGCTGGTGAAGCCTGGCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.01	0.0
175-179	0.0	0.0	0.0	0.045	0.0
180-184	0.0	0.0	0.0	0.08	0.0
185-189	0.0	0.0	0.0	0.125	0.0
190-194	0.0	0.0	0.0	0.14500000000000002	0.0
195-199	0.0	0.0	0.0	0.15	0.0
200-204	0.0	0.0	0.0	0.15	0.0
205-209	0.0	0.0	0.0	0.15	0.0
210-214	0.0	0.0	0.0	0.15	0.0
215-219	0.0	0.0	0.0	0.15	0.0
220-224	0.0	0.0	0.0	0.15	0.0
225-229	0.0	0.0	0.0	0.15	0.0
230-234	0.0	0.0	0.0	0.15	0.0
235-239	0.0	0.0	0.0	0.15	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCT	10	0.0012667647	254.5065	245
ATGGAGC	10	0.0012667647	254.5065	245
GAGGTGC	115	0.0	244.96251	5
GAGGTGA	25	1.3369572E-9	244.96251	5
GTAGAGG	10	0.0014224675	244.96251	2
TTGCAGG	15	1.4099249E-5	244.96251	2
GTGTCAG	10	0.0014224675	244.96251	1
CTGAGGT	25	1.3369572E-9	244.96251	3
GTGCAGG	30	1.2732926E-11	244.96251	8
GGGCAGG	10	0.0014224675	244.96251	2
GTGCAAC	15	1.4099249E-5	244.96251	8
TCAGGTT	50	0.0	244.96251	4
TCAGGTC	20	1.377739E-7	244.96251	4
TGCAACT	15	1.4099249E-5	244.96251	9
GCAGGTT	40	0.0	244.96251	4
GCAGGTC	20	1.377739E-7	244.96251	4
AGAGGTG	20	1.377739E-7	244.96251	4
GGTCCAC	15	1.4099249E-5	244.96251	7
GGTCCAA	15	1.4099249E-5	244.96251	7
GCGGTTC	10	0.0014224675	244.96251	4
>>END_MODULE
SRR32999411 read2 length is 247-251 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999411_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	247-251
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8255	33.0	32.0	33.0	32.0	34.0
2	32.18825	33.0	32.0	33.0	32.0	34.0
3	32.23175	33.0	32.0	33.0	32.0	34.0
4	32.03375	33.0	32.0	33.0	32.0	34.0
5	32.3435	33.0	32.0	33.0	32.0	34.0
6	34.74	37.0	33.0	37.0	33.0	37.0
7	34.092	34.0	33.0	37.0	32.0	37.0
8	34.68575	35.0	33.0	37.0	33.0	37.0
9	35.609	37.0	35.0	37.0	33.0	37.0
10-14	35.087	35.8	35.2	36.2	34.0	36.4
15-19	36.66275	38.0	37.2	38.0	35.6	38.0
20-24	37.33924999999999	38.4	37.8	38.4	36.8	38.4
25-29	37.62049999999999	38.6	37.8	38.6	37.0	38.8
30-34	37.728500000000004	38.8	38.2	38.8	37.4	38.8
35-39	37.43555	38.6	38.2	38.6	36.6	38.6
40-44	38.08815	39.0	39.0	39.0	37.6	39.0
45-49	37.3343	38.2	38.2	38.2	37.2	38.2
50-54	38.00435	38.8	38.8	38.8	38.0	38.8
55-59	37.82355	38.8	38.4	38.8	37.4	38.8
60-64	37.26635	38.0	38.0	38.2	36.4	38.6
65-69	37.6305	38.6	38.6	38.6	36.8	39.0
70-74	37.63945	38.4	38.4	38.8	37.2	38.8
75-79	38.010349999999995	39.0	38.6	39.0	37.6	39.0
80-84	37.965250000000005	38.8	38.4	39.0	37.6	39.0
85-89	37.0887	38.2	37.6	38.4	36.4	38.4
90-94	37.59349999999999	38.8	38.4	39.0	37.0	39.0
95-99	37.577099999999994	38.6	38.6	39.0	37.2	39.0
100-104	37.2389	38.4	38.0	39.0	36.0	39.0
105-109	37.0585	38.6	38.0	39.0	35.6	39.0
110-114	37.4091	38.8	38.2	38.8	36.6	39.0
115-119	37.19775	38.4	38.0	39.0	36.6	39.0
120-124	37.53574999999999	39.0	38.4	39.0	37.0	39.0
125-129	37.399449999999995	39.0	38.0	39.0	36.8	39.0
130-134	37.450599999999994	39.0	38.0	39.0	36.8	39.0
135-139	37.47205	39.0	38.2	39.0	37.0	39.0
140-144	37.4101	39.0	38.0	39.0	36.8	39.0
145-149	37.559900000000006	39.0	38.8	39.0	37.0	39.0
150-154	37.259750000000004	39.0	38.0	39.0	36.4	39.0
155-159	37.372949999999996	39.0	38.2	39.0	36.8	39.0
160-164	37.590999999999994	39.0	38.6	39.0	36.8	39.0
165-169	37.186749999999996	39.0	38.0	39.0	36.0	39.0
170-174	37.51795	39.0	38.4	39.0	37.0	39.0
175-179	37.332049999999995	39.0	38.0	39.0	36.4	39.0
180-184	37.0479	39.0	38.0	39.0	35.2	39.0
185-189	37.155699999999996	39.0	38.0	39.0	35.2	39.0
190-194	37.2142	39.0	38.0	39.0	35.8	39.0
195-199	36.98595	39.0	38.0	39.0	34.2	39.0
200-204	36.824200000000005	39.0	38.0	39.0	34.0	39.0
205-209	36.7577	39.0	37.8	39.0	33.6	39.0
210-214	36.5166	38.0	37.2	39.0	33.6	39.0
215-219	35.977199999999996	38.0	37.0	38.0	33.2	39.0
220-224	35.8245	38.0	37.0	38.0	32.8	38.0
225-229	35.998749999999994	38.0	37.0	38.0	32.8	38.0
230-234	35.7616	38.0	37.0	38.0	32.4	38.0
235-239	35.2076	37.8	37.0	38.0	27.4	38.0
240-244	35.346050000000005	37.6	37.0	38.0	31.6	38.0
245-249	34.81883754058036	37.0	36.2	38.0	27.0	38.0
250-251	33.168725890628544	37.0	31.5	37.5	20.0	37.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1102	1	0.0
1102	2	0.0
1102	3	0.0
1102	4	0.0
1102	5	0.0
1102	6	0.0
1102	7	0.0
1102	8	0.0
1102	9	0.0
1102	10-14	0.0
1102	15-19	0.0
1102	20-24	0.0
1102	25-29	0.0
1102	30-34	0.0
1102	35-39	0.0
1102	40-44	0.0
1102	45-49	0.0
1102	50-54	0.0
1102	55-59	0.0
1102	60-64	0.0
1102	65-69	0.0
1102	70-74	0.0
1102	75-79	0.0
1102	80-84	0.0
1102	85-89	0.0
1102	90-94	0.0
1102	95-99	0.0
1102	100-104	0.0
1102	105-109	0.0
1102	110-114	0.0
1102	115-119	0.0
1102	120-124	0.0
1102	125-129	0.0
1102	130-134	0.0
1102	135-139	0.0
1102	140-144	0.0
1102	145-149	0.0
1102	150-154	0.0
1102	155-159	0.0
1102	160-164	0.0
1102	165-169	0.0
1102	170-174	0.0
1102	175-179	0.0
1102	180-184	0.0
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-251	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	22.0
16	37.0
17	15.0
18	9.0
19	4.0
20	3.0
21	0.0
22	3.0
23	2.0
24	4.0
25	5.0
26	8.0
27	17.0
28	19.0
29	14.0
30	28.0
31	27.0
32	36.0
33	46.0
34	89.0
35	127.0
36	250.0
37	1114.0
38	2120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.775	18.65	33.2	21.375
2	26.25	19.175	32.05	22.525000000000002
3	28.425	19.0	32.0	20.575
4	28.449999999999996	19.650000000000002	30.475	21.425
5	97.02499999999999	1.275	1.4500000000000002	0.25
6	1.675	97.45	0.375	0.5
7	96.39999999999999	0.4	1.675	1.525
8	0.675	0.075	3.025	96.22500000000001
9	1.325	0.15	95.975	2.55
10-14	59.855000000000004	0.165	20.330000000000002	19.650000000000002
15-19	59.41	19.475	20.735	0.38
20-24	21.815	19.595000000000002	38.61	19.98
25-29	60.11	19.830000000000002	19.919999999999998	0.13999999999999999
30-34	59.24	19.685	20.995	0.08
35-39	60.07	19.869999999999997	19.96	0.1
40-44	1.01	0.19	78.105	20.695
45-49	39.739999999999995	20.71	0.65	38.9
50-54	1.59	18.98	39.645	39.785
55-59	59.285	39.165	0.485	1.065
60-64	54.625	20.605	5.1	19.67
65-69	25.900000000000002	23.615	22.335	28.15
70-74	58.865	5.489999999999999	34.894999999999996	0.75
75-79	16.875	0.055	31.85	51.22
80-84	25.035	3.535	11.360000000000001	60.07
85-89	20.365	39.725	8.74	31.169999999999998
90-94	19.945	36.275	19.34	24.44
95-99	22.005	38.585	19.355	20.055
100-104	19.134999999999998	20.544999999999998	23.595	36.725
105-109	18.8	21.465	23.189999999999998	36.545
110-114	17.335	20.424999999999997	24.87	37.37
115-119	17.93	24.87	31.255	25.945
120-124	19.11	12.83	45.645	22.415
125-129	21.310000000000002	35.855	11.63	31.205
130-134	9.885	45.5	30.769999999999996	13.844999999999999
135-139	32.574999999999996	21.09	22.400000000000002	23.935000000000002
140-144	29.805	38.4	10.115	21.68
145-149	15.024999999999999	8.545	33.395	43.035000000000004
150-154	37.095	25.915	26.224999999999998	10.764999999999999
155-159	34.31	18.945	21.46	25.285000000000004
160-164	24.185000000000002	7.915	50.13999999999999	17.76
165-169	39.155	21.23	22.08	17.535
170-174	36.18	14.685	29.695	19.439999999999998
175-179	36.394999999999996	20.560000000000002	28.63	14.415
180-184	39.745000000000005	9.585	34.72	15.950000000000001
185-189	36.975	30.404999999999998	24.925	7.695
190-194	34.644999999999996	19.509999999999998	37.31	8.535
195-199	23.01	19.470000000000002	39.839999999999996	17.68
200-204	22.259999999999998	20.485	22.74	34.515
205-209	19.31	25.555	41.29	13.844999999999999
210-214	38.96	9.315	21.884999999999998	29.84
215-219	21.55	6.265	27.77	44.415
220-224	23.74	31.275	36.165000000000006	8.82
225-229	9.27	9.135	54.02	27.575
230-234	27.555000000000003	24.525	39.184999999999995	8.735
235-239	17.095	22.5	36.89	23.515
240-244	31.580000000000002	33.129999999999995	25.865	9.425
245-249	8.773070574701146	40.644225478917626	22.25278847596659	28.329915470414647
250-251	10.831099195710456	2.734584450402145	70.72386058981233	15.710455764075068
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.3333333333333333
36	0.3333333333333333
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.3333333333333333
45	2.333333333333333
46	10.0
47	14.666666666666675
48	45.33333333333338
49	108.99999999999953
50	85.99999999999986
51	43.333333333333364
52	85.3333333333332
53	264.00000000000244
54	648.0000000000038
55	539.3333333333247
56	112.99999999999947
57	18.666666666666668
58	4.0
59	3.0
60	2.6666666666666665
61	1.6666666666666665
62	2.6666666666666665
63	2.6666666666666665
64	3.0
65	1.9999999999999998
66	1.9999999999999998
67	1.3333333333333333
68	1.9999999999999998
69	1.3333333333333333
70	1.0
71	0.6666666666666666
72	0.6666666666666666
73	0.3333333333333333
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
247	3.0
248	1.0
249	96.0
250	2205.0
251	1695.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.0672268907563	14.174999999999999
2	1.3205282112845138	0.5499999999999999
3	0.6002400960384154	0.375
4	0.6002400960384154	0.5
5	1.56062424969988	1.625
6	1.2004801920768309	1.5
7	1.680672268907563	2.45
8	3.12124849939976	5.2
9	2.0408163265306123	3.8249999999999997
>10	19.807923169267706	69.8
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	34	0.8500000000000001	No Hit
TGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	33	0.8250000000000001	No Hit
TTTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	33	0.8250000000000001	No Hit
GTTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	31	0.775	No Hit
GGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	31	0.775	No Hit
GGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	31	0.775	No Hit
TTCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	29	0.7250000000000001	No Hit
TTATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	29	0.7250000000000001	No Hit
TGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	29	0.7250000000000001	No Hit
GTGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	28	0.7000000000000001	No Hit
TTCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	28	0.7000000000000001	No Hit
GTTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
GTGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
CGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
TTTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	26	0.65	No Hit
CTTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	25	0.625	No Hit
TGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	25	0.625	No Hit
CTTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	25	0.625	No Hit
TCTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	25	0.625	No Hit
GTGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
TATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
ATTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
TTAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
TTGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
CGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
GCTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	24	0.6	No Hit
TACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
CGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
TCGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
CCTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	23	0.575	No Hit
GCTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
CTGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TCCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TTTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
TTGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	22	0.5499999999999999	No Hit
GTATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	21	0.525	No Hit
CCTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	21	0.525	No Hit
TTGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	21	0.525	No Hit
AGTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	21	0.525	No Hit
TGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TCTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GTCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
ATGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
ATGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TCGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GTTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
TCGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
GCTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	20	0.5	No Hit
ATTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
GCGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
TCCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
AGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
CGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	19	0.475	No Hit
GTTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
TAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
GTCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
AGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	18	0.44999999999999996	No Hit
GATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
ATCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
TATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
TATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
CTGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
GCGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
CCGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	17	0.42500000000000004	No Hit
TTCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
CCTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
CTAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
CTTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TACTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
ATCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
GCCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TCAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	16	0.4	No Hit
TAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
TCTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CCGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
TGTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GCCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
TGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
CTCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GCTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	15	0.375	No Hit
GGTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TCGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
AATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
GAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TCTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TTCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TTACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
TAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
ACGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
CATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
GGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	14	0.35000000000000003	No Hit
GAGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ACGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ATCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ATGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GTAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CTCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CTAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CTATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
TAACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
CAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GGCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
ACTTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
AAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
AGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	13	0.325	No Hit
GGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
GGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TCACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TGCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TTGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TAGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
GTGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TCATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
GGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CGGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
AGGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
AGTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
CAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	12	0.3	No Hit
TTAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GTCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
ACTGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
TACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CTTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CCTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GCGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CTGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
ATTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CAGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
CGGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
TCCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
AAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
AACTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	11	0.27499999999999997	No Hit
GGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GTACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CATCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
TGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GCCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
TAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
ACATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
CTCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
AGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
ACTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
AGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
GGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	10	0.25	No Hit
TGAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GCCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CGAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GTAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
ACGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CGACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
ACCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
ATTCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AAGTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
GCATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AATTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CCGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CAGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
CTACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AAGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
AAGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	9	0.22499999999999998	No Hit
TCCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CCCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AGATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GTCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CTGCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ACCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CCCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ATGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CCCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ATAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AGTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ACTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ATCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CGCAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CGCCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ATATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
TCAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
CGCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
GCGGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
AACGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	8	0.2	No Hit
ACAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ACAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
GATGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ACCGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CCACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
GAAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
AGGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CGAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CGCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
AACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
AATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CGTAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
GACTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
CATAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	7	0.17500000000000002	No Hit
ACCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
CAACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
GCAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
CCAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
TACAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
ACACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
AAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
GAATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
AAACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
GACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	6	0.15	No Hit
ATAGGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
GAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
GGAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
ACGAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
GCACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
CCCTGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
CCATGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
GCAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
ATACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
AAAAGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
AACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
GAACGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
CACCGAGCTCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.01	0.0
175-179	0.0	0.0	0.0	0.045	0.0
180-184	0.0	0.0	0.0	0.08	0.0
185-189	0.0	0.0	0.0	0.125	0.0
190-194	0.0	0.0	0.0	0.14500000000000002	0.0
195-199	0.0	0.0	0.0	0.15	0.0
200-204	0.0	0.0	0.0	0.15	0.0
205-209	0.0	0.0	0.0	0.15	0.0
210-214	0.0	0.0	0.0	0.15	0.0
215-219	0.0	0.0	0.0	0.15	0.0
220-224	0.0	0.0	0.0	0.15	0.0
225-229	0.0	0.0	0.0	0.15	0.0
230-234	0.0	0.0	0.0	0.15	0.0
235-239	0.0	0.0	0.0	0.15	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTTTC	10	1.2485386E-4	543.1666	245
AATTATT	10	1.2485386E-4	543.1666	245
CAGGATC	25	6.706614E-9	434.53336	245
TAGGATT	15	4.2084878E-4	362.1111	245
AATATGT	55	1.8189894E-12	296.27274	245
CATAATT	20	9.96306E-4	271.5833	245
TGTGAGC	10	0.0014318539	244.425	2
CAAGAGC	10	0.0014318539	244.425	2
GGTGAGC	15	1.4223389E-5	244.425	2
CCGGAGC	10	0.0014318539	244.425	2
GAGGAGC	10	0.0014318539	244.425	2
AAGAGCT	15	1.4223389E-5	244.425	3
TGCGAGC	25	1.3533281E-9	244.425	2
AGTGGAG	10	0.0014318539	244.425	1
CCTTGAG	15	1.4223389E-5	244.425	1
TATTGAG	10	0.0014318539	244.425	1
GATGAGC	15	1.4223389E-5	244.425	2
AGGGAGC	10	0.0014318539	244.425	2
GGGAGCT	15	1.4223389E-5	244.425	3
AGAGCTC	75	0.0	244.425	4
>>END_MODULE
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
Read 56009 spots for SRR32999411.sra
Written 56009 spots for SRR32999411.sra
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
Read 56005 spots for SRR32999411.sra
Written 56005 spots for SRR32999411.sra
SRR ids: ['SRR32999411.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_lvu2bheb
SRR32999411.sra spots: 448044
blocks: [[1, 56005], [56006, 112010], [112011, 168015], [168016, 224020], [224021, 280025], [280026, 336030], [336031, 392035], [392036, 448044]]
SRR32999411 file size 254280
SRR32999411 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32999411 SRR32999411_1.fastq SRR32999411_2.fastq
Input file:	SRR32999411_1.fastq
Paired file:	SRR32999411_2.fastq
trimmed:	SRR32999411-trimmed-pair1.fastq, SRR32999411-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 17 17:31:30 2026 >> started

Tue Mar 17 17:31:30 2026 >> done (0.648s)
448044 read pairs processed; of these:
     0 ( 0.00%) short read pairs filtered out after trimming by size control
     0 ( 0.00%) empty read pairs filtered out after trimming by size control
448044 (100.00%) read pairs available; of these:
448044 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
248	   209	  0.05%
249	  1244	  0.28%
250	267032	 59.60%
251	179559	 40.08%
448044 reads passed initial QC


criterion=sequence-density
sequence-density=0.02
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=2
prefix-density=0.00
prefix-fanout=1.0
sequence=GAGCTCGTGGGAGTGTC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=2
fanout-score=1.00
fanout-score-rank=1
prefix-density=0.02
prefix-fanout=1.0
sequence=GAGTGTCAGTGGG
SRR32999411 testing PE reads STAR mapping to Ensembl genome
Unpaired reads removal
                                 Started job on |	Mar 17 17:32:31
                             Started mapping on |	Mar 17 17:32:31
                                    Finished on |	Mar 17 17:33:14
       Mapping speed, Million of reads per hour |	37.51

                          Number of input reads |	448044
                      Average input read length |	477
                                    UNIQUE READS:
                   Uniquely mapped reads number |	393305
                        Uniquely mapped reads % |	87.78%
                          Average mapped length |	410.68
                       Number of splices: Total |	543085
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	368754
                       Number of splices: GC/AG |	2778
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	171553
                      Mismatch rate per base, % |	1.43%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.10
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	12049
             % of reads mapped to multiple loci |	2.69%
        Number of reads mapped to too many loci |	65
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.67%
                     % of reads unmapped: other |	4.84%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	42690	42690	42690
N_multimapping	12049	12049	12049
N_noFeature	0	0	393215
N_ambiguous	191232	191232	0
UnstrandedReadsAssigned:202073 PositiveStrandReadsAssigned:202073 NegativeStrandReadsAssigned:90
Dataset is classified positive stranded
MeadianReadLen=231 20thPercentileLength=231 echo kmer=227
SRR32999411 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR32999411-trimmed-pair1.fastq
                             SRR32999411-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 448,044 reads, 9,660 reads pseudoaligned
[quant] estimated average fragment length: 0
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 200 rounds

 109283 SRR32999411.ke.tsv
  52636 SRR32999411.se.tsv
 161919 total
==> SRR32999411.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR32999411.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32999411 completed mapping pipeline successfully
