Starting /dee2/code/volunteer_pipeline.sh SRR32999354
    current disk space = 1064970912
    free memory = 194085496 
SRR32999354 SRAfilesize
bdd554121ccbbdd50165e2c3544e57af  SRR32999354.sra
SRR32999354.sra file validated
SRR32999354 is paired end
SRR32999354 is conventional basespace
SRR32999354 read1 length is 247-251 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999354_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	247-251
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094	33.0	32.0	33.0	32.0	34.0
2	32.4515	33.0	32.0	34.0	32.0	34.0
3	32.4025	33.0	32.0	34.0	32.0	34.0
4	32.56525	33.0	32.0	34.0	32.0	34.0
5	32.38525	33.0	32.0	34.0	32.0	34.0
6	35.23725	37.0	33.0	37.0	33.0	37.0
7	35.278	37.0	33.0	37.0	33.0	37.0
8	34.92	34.0	33.0	37.0	33.0	37.0
9	35.82975	37.0	34.0	37.0	33.0	37.0
10-14	36.68145	37.4	36.6	37.4	36.0	37.4
15-19	37.4048	38.0	38.0	38.0	37.0	38.0
20-24	37.9987	38.4	38.4	38.4	38.0	38.4
25-29	38.284949999999995	38.8	38.6	39.0	38.0	39.0
30-34	37.4636	38.2	37.8	39.0	35.8	39.0
35-39	38.12375	39.0	38.4	39.0	37.0	39.0
40-44	38.03765	39.0	38.0	39.0	37.2	39.0
45-49	38.2567	38.8	38.4	38.8	37.6	39.0
50-54	37.650600000000004	38.2	38.2	38.4	37.0	39.0
55-59	38.4444	39.0	39.0	39.0	37.6	39.0
60-64	38.13505	39.0	38.6	39.0	37.2	39.0
65-69	38.71905	39.0	39.0	39.0	38.8	39.0
70-74	38.013450000000006	38.8	38.6	39.0	37.2	39.0
75-79	38.563649999999996	39.0	39.0	39.0	38.2	39.0
80-84	38.446600000000004	39.0	39.0	39.0	37.8	39.0
85-89	38.0279	39.0	38.2	39.0	37.2	39.0
90-94	38.04015	39.0	38.6	39.0	37.4	39.0
95-99	37.9109	39.0	38.4	39.0	37.0	39.0
100-104	37.749750000000006	39.0	38.0	39.0	36.8	39.0
105-109	37.78065	39.0	38.2	39.0	36.4	39.0
110-114	38.3724	39.0	38.4	39.0	37.6	39.0
115-119	37.7691	38.8	37.8	38.8	36.8	39.0
120-124	37.739	38.8	38.0	38.8	36.8	39.0
125-129	37.5822	38.4	38.0	38.6	36.6	39.0
130-134	37.7298	38.8	38.0	39.0	37.0	39.0
135-139	37.64975	38.4	38.0	38.8	36.6	39.0
140-144	37.88935	39.0	38.2	39.0	37.0	39.0
145-149	37.97625000000001	38.8	38.6	38.8	37.2	39.0
150-154	37.162800000000004	38.8	38.0	39.0	34.8	39.0
155-159	37.829049999999995	39.0	38.2	39.0	36.8	39.0
160-164	37.46425	39.0	38.2	39.0	35.8	39.0
165-169	36.97235	38.8	37.6	39.0	34.4	39.0
170-174	37.22745	38.6	37.8	39.0	35.8	39.0
175-179	37.2899	39.0	37.8	39.0	35.8	39.0
180-184	37.201800000000006	39.0	37.6	39.0	35.0	39.0
185-189	36.82900000000001	38.8	37.8	39.0	34.2	39.0
190-194	36.89455	38.0	37.4	39.0	34.0	39.0
195-199	37.3735	39.0	38.0	39.0	35.4	39.0
200-204	36.82855	38.2	37.4	38.2	34.4	39.0
205-209	36.9113	38.6	37.8	38.6	34.2	39.0
210-214	36.54895	38.0	37.2	38.8	33.4	38.8
215-219	36.725	38.0	37.0	39.0	34.0	39.0
220-224	36.233549999999994	38.0	37.0	38.0	31.8	39.0
225-229	36.3655	38.0	37.0	38.0	33.4	39.0
230-234	36.176	38.0	37.0	38.0	33.2	38.4
235-239	35.9112	38.0	37.0	38.0	32.6	38.0
240-244	35.92485	37.4	37.0	38.0	32.6	38.0
245-249	35.74201296041423	37.0	37.0	38.0	33.4	38.0
250-251	33.76725106959279	36.5	35.0	37.5	23.5	37.5
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1102	1	0.0
1102	2	0.0
1102	3	0.0
1102	4	0.0
1102	5	0.0
1102	6	0.0
1102	7	0.0
1102	8	0.0
1102	9	0.0
1102	10-14	0.0
1102	15-19	0.0
1102	20-24	0.0
1102	25-29	0.0
1102	30-34	0.0
1102	35-39	0.0
1102	40-44	0.0
1102	45-49	0.0
1102	50-54	0.0
1102	55-59	0.0
1102	60-64	0.0
1102	65-69	0.0
1102	70-74	0.0
1102	75-79	0.0
1102	80-84	0.0
1102	85-89	0.0
1102	90-94	0.0
1102	95-99	0.0
1102	100-104	0.0
1102	105-109	0.0
1102	110-114	0.0
1102	115-119	0.0
1102	120-124	0.0
1102	125-129	0.0
1102	130-134	0.0
1102	135-139	0.0
1102	140-144	0.0
1102	145-149	0.0
1102	150-154	0.0
1102	155-159	0.0
1102	160-164	0.0
1102	165-169	0.0
1102	170-174	0.0
1102	175-179	0.0
1102	180-184	0.0
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-251	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	2.0
25	1.0
26	8.0
27	13.0
28	7.0
29	20.0
30	27.0
31	32.0
32	64.0
33	69.0
34	120.0
35	199.0
36	354.0
37	1000.0
38	2081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.825	12.25	31.2	19.725
2	34.050000000000004	11.625	35.075	19.25
3	34.425	12.5	32.300000000000004	20.775
4	33.550000000000004	12.9	33.875	19.675
5	49.15	2.0	0.35000000000000003	48.5
6	2.55	97.225	0.075	0.15
7	94.825	1.15	3.125	0.8999999999999999
8	96.925	0.15	2.875	0.05
9	1.0	0.075	97.7	1.225
10-14	24.785	22.58	10.41	42.225
15-19	36.555	19.564999999999998	25.545	18.335
20-24	23.69	20.225	20.925	35.160000000000004
25-29	66.49000000000001	8.37	20.9	4.24
30-34	38.815	26.840000000000003	14.04	20.305
35-39	32.385000000000005	3.4549999999999996	43.805	20.355
40-44	39.905	37.125	0.36	22.61
45-49	56.28999999999999	7.805	15.795	20.11
50-54	21.38	3.25	26.07	49.3
55-59	32.34	37.245	21.25	9.165
60-64	31.25	25.840000000000003	31.71	11.200000000000001
65-69	19.950000000000003	0.745	38.185	41.120000000000005
70-74	32.45	38.98	2.7199999999999998	25.85
75-79	2.54	1.43	56.464999999999996	39.565
80-84	38.15	15.645000000000001	28.34	17.865000000000002
85-89	2.87	32.865	19.43	44.835
90-94	4.505	33.375	38.76	23.36
95-99	18.47	39.34	18.115000000000002	24.075
100-104	19.88	31.740000000000002	28.895	19.485
105-109	21.759999999999998	27.48	22.015	28.744999999999997
110-114	59.665	1.91	37.695	0.73
115-119	29.17	44.025	2.1999999999999997	24.605
120-124	51.625	38.505	3.245	6.625
125-129	32.435	14.67	18.95	33.945
130-134	39.26	43.85	2.39	14.499999999999998
135-139	30.97	2.855	34.335	31.840000000000003
140-144	55.835	23.875	20.11	0.18
145-149	42.089999999999996	18.265	38.5	1.145
150-154	42.405	35.35	13.255	8.99
155-159	8.85	39.574999999999996	50.135	1.44
160-164	16.395	11.84	35.3	36.464999999999996
165-169	33.14	37.055	21.33	8.475000000000001
170-174	48.010000000000005	26.479999999999997	23.86	1.6500000000000001
175-179	2.77	38.865	36.980000000000004	21.385
180-184	5.465	44.355	26.25	23.93
185-189	20.625	37.55	12.08	29.744999999999997
190-194	32.18	41.58	22.11	4.130000000000001
195-199	22.915	33.545	19.009999999999998	24.529999999999998
200-204	37.6	40.915	1.44	20.044999999999998
205-209	35.22	21.645	4.015	39.12
210-214	12.885	26.634999999999998	32.824999999999996	27.655
215-219	20.085	23.925	19.73	36.26
220-224	18.91	56.925000000000004	3.1399999999999997	21.025
225-229	3.515	29.095	22.18	45.21
230-234	12.945	31.790000000000003	17.24	38.025
235-239	18.17	37.830000000000005	2.5149999999999997	41.485
240-244	3.65	20.79	24.5	51.06
245-249	24.36487297459492	31.266253250650127	20.954190838167634	23.414682936587315
250-251	32.17039069645891	11.224356461518358	10.16594799425062	46.439304847772114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.3333333333333333
34	0.3333333333333333
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	7.33333333333333
46	36.333333333333314
47	48.666666666666735
48	52.00000000000009
49	130.666666666666
50	291.0000000000009
51	459.3333333333247
52	474.3333333333238
53	319.66666666666595
54	139.9999999999996
55	36.333333333333314
56	5.999999999999998
57	0.3333333333333333
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
247	1.0
248	2.0
249	22.0
250	297.0
251	3678.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.19999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.64075630252101	91.05
2	3.8340336134453783	7.3
3	0.3939075630252101	1.125
4	0.10504201680672269	0.4
5	0.026260504201680673	0.125
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTCAGGTTCAGCTGCAGCAGTCTGTGGCAGAGCTTGTGAGGCCAGGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.005	0.0
165-169	0.0	0.0	0.0	0.025	0.0
170-174	0.0	0.0	0.0	0.025	0.0
175-179	0.0	0.0	0.0	0.025	0.0
180-184	0.0	0.0	0.0	0.025	0.0
185-189	0.0	0.0	0.0	0.025	0.0
190-194	0.0	0.0	0.0	0.025	0.0
195-199	0.0	0.0	0.0	0.025	0.0
200-204	0.0	0.0	0.0	0.025	0.0
205-209	0.0	0.0	0.0	0.025	0.0
210-214	0.0	0.0	0.0	0.025	0.0
215-219	0.0	0.0	0.0	0.025	0.0
220-224	0.0	0.0	0.0	0.025	0.0
225-229	0.0	0.0	0.0	0.025	0.0
230-234	0.0	0.0	0.0	0.025	0.0
235-239	0.0	0.0	0.0	0.025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTCAG	10	0.0014229023	244.9375	1
CTGAGGT	20	1.3784484E-7	244.9375	3
CGGAGGT	10	0.0014229023	244.9375	3
CCCAGGT	10	0.0014229023	244.9375	3
GTGCAGG	15	1.4104995E-5	244.9375	8
TCAGGTT	50	0.0	244.9375	4
TCAGGTC	20	1.3784484E-7	244.9375	4
GCAGGTC	15	1.4104995E-5	244.9375	4
AGAGGTG	10	0.0014229023	244.9375	4
GGTCCAC	10	0.0014229023	244.9375	7
GGTCCAA	10	0.0014229023	244.9375	7
GATGTGC	15	1.4104995E-5	244.9375	5
TGTCAGG	10	0.0014229023	244.9375	2
GTTCAGG	10	0.0014229023	244.9375	2
GGGAGGT	30	1.2732926E-11	244.9375	3
GCCAGGT	15	1.4104995E-5	244.9375	3
ATGTGCA	15	1.4104995E-5	244.9375	6
TGCCAGG	10	0.0014229023	244.9375	2
CTCAGGT	25	1.3369572E-9	244.9375	3
TCTGAGG	10	0.0014229023	244.9375	2
>>END_MODULE
SRR32999354 read2 length is 247-251 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32999354_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	247-251
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.5955	32.0	30.0	33.0	18.0	33.0
2	30.311	32.0	32.0	33.0	18.0	33.0
3	30.4985	32.0	32.0	33.0	18.0	33.0
4	30.66475	32.0	32.0	33.0	29.0	33.0
5	31.825	32.0	32.0	33.0	32.0	33.0
6	33.735	35.0	32.0	37.0	31.0	37.0
7	34.31225	35.0	33.0	37.0	32.0	37.0
8	34.20125	33.0	33.0	37.0	32.0	37.0
9	34.2265	33.0	33.0	37.0	32.0	37.0
10-14	34.0852	35.0	34.2	35.8	32.6	35.8
15-19	35.928900000000006	37.0	36.0	38.0	33.6	38.0
20-24	37.077	38.4	37.8	38.4	35.4	38.4
25-29	37.685950000000005	38.6	38.2	38.8	36.6	38.8
30-34	36.54540000000001	38.2	36.8	38.4	34.4	38.6
35-39	37.43915	38.8	38.0	39.0	36.4	39.0
40-44	37.94395	39.0	38.8	39.0	37.2	39.0
45-49	37.0595	38.6	37.6	38.8	35.4	39.0
50-54	37.3162	38.2	37.6	39.0	35.8	39.0
55-59	36.96805	38.8	37.6	39.0	34.4	39.0
60-64	36.52335	38.0	36.8	38.8	33.2	39.0
65-69	38.022450000000006	39.0	38.6	39.0	37.2	39.0
70-74	37.6169	38.8	38.2	38.8	36.6	38.8
75-79	37.00744999999999	38.6	37.6	38.6	35.8	38.6
80-84	37.06245	39.0	37.8	39.0	34.6	39.0
85-89	36.17695	38.0	37.0	39.0	31.6	39.0
90-94	35.8786	38.0	37.0	39.0	30.0	39.0
95-99	35.9187	38.0	37.0	39.0	30.0	39.0
100-104	35.9751	38.2	37.0	39.0	30.0	39.0
105-109	36.06685	38.4	37.0	39.0	30.8	39.0
110-114	36.173950000000005	38.8	37.0	39.0	30.8	39.0
115-119	36.1083	38.8	37.0	39.0	30.4	39.0
120-124	36.024950000000004	38.8	37.0	39.0	29.8	39.0
125-129	35.82435	38.0	37.0	39.0	29.0	39.0
130-134	35.78575	38.4	37.0	39.0	28.2	39.0
135-139	35.7672	38.8	37.0	39.0	27.0	39.0
140-144	35.74125	38.6	37.0	39.0	27.0	39.0
145-149	35.751149999999996	38.6	37.0	39.0	27.2	39.0
150-154	35.91435	38.8	37.0	39.0	27.6	39.0
155-159	35.485	38.2	37.0	39.0	26.8	39.0
160-164	35.4958	38.4	36.8	39.0	26.8	39.0
165-169	35.245599999999996	38.2	36.2	39.0	24.2	39.0
170-174	35.2827	38.0	36.4	39.0	26.0	39.0
175-179	35.1955	38.0	36.2	39.0	23.2	39.0
180-184	35.02705	38.0	35.8	39.0	22.8	39.0
185-189	35.038050000000005	38.0	36.4	39.0	24.4	39.0
190-194	34.74595000000001	38.0	35.2	39.0	22.2	39.0
195-199	34.4344	38.0	34.0	38.8	16.8	39.0
200-204	34.25705	38.0	34.0	38.0	16.8	39.0
205-209	34.083549999999995	37.4	33.6	38.0	18.6	39.0
210-214	33.9747	37.0	33.8	38.0	15.0	38.2
215-219	33.8473	37.0	33.4	38.0	18.6	38.0
220-224	33.5382	37.0	33.0	38.0	15.0	38.0
225-229	33.5397	37.0	33.0	38.0	15.0	38.0
230-234	33.080600000000004	37.0	33.0	37.4	15.0	38.0
235-239	32.56	37.0	33.0	37.0	14.2	38.0
240-244	32.49125	37.0	33.0	37.0	14.0	37.8
245-249	32.02165638869637	37.0	32.8	37.0	14.0	37.0
250-251	29.486566368104224	34.5	23.0	37.0	14.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1102	1	0.0
1102	2	0.0
1102	3	0.0
1102	4	0.0
1102	5	0.0
1102	6	0.0
1102	7	0.0
1102	8	0.0
1102	9	0.0
1102	10-14	0.0
1102	15-19	0.0
1102	20-24	0.0
1102	25-29	0.0
1102	30-34	0.0
1102	35-39	0.0
1102	40-44	0.0
1102	45-49	0.0
1102	50-54	0.0
1102	55-59	0.0
1102	60-64	0.0
1102	65-69	0.0
1102	70-74	0.0
1102	75-79	0.0
1102	80-84	0.0
1102	85-89	0.0
1102	90-94	0.0
1102	95-99	0.0
1102	100-104	0.0
1102	105-109	0.0
1102	110-114	0.0
1102	115-119	0.0
1102	120-124	0.0
1102	125-129	0.0
1102	130-134	0.0
1102	135-139	0.0
1102	140-144	0.0
1102	145-149	0.0
1102	150-154	0.0
1102	155-159	0.0
1102	160-164	0.0
1102	165-169	0.0
1102	170-174	0.0
1102	175-179	0.0
1102	180-184	0.0
1102	185-189	0.0
1102	190-194	0.0
1102	195-199	0.0
1102	200-204	0.0
1102	205-209	0.0
1102	210-214	0.0
1102	215-219	0.0
1102	220-224	0.0
1102	225-229	0.0
1102	230-234	0.0
1102	235-239	0.0
1102	240-244	0.0
1102	245-249	0.0
1102	250-251	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	16.0
17	15.0
18	13.0
19	8.0
20	3.0
21	6.0
22	3.0
23	12.0
24	30.0
25	43.0
26	38.0
27	43.0
28	67.0
29	92.0
30	82.0
31	119.0
32	136.0
33	152.0
34	202.0
35	313.0
36	557.0
37	1478.0
38	567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.575	22.225	32.5	21.7
2	22.325	20.025000000000002	33.074999999999996	24.575
3	24.575	20.375	34.575	20.474999999999998
4	27.125	18.575	33.175	21.125
5	97.675	1.6500000000000001	0.15	0.525
6	2.8000000000000003	96.775	0.025	0.4
7	97.3	1.925	0.5499999999999999	0.22499999999999998
8	0.475	97.15	0.15	2.225
9	3.225	0.3	0.25	96.22500000000001
10-14	79.415	19.63	0.47000000000000003	0.485
15-19	59.275	40.07	0.32	0.335
20-24	0.7250000000000001	38.615	40.63	20.03
25-29	59.925	20.599999999999998	19.39	0.08499999999999999
30-34	40.025	38.99	1.225	19.759999999999998
35-39	20.11	20.169999999999998	38.74	20.979999999999997
40-44	20.05	0.905	39.77	39.275
45-49	53.455	39.98	0.22999999999999998	6.335
50-54	28.23	25.495	26.419999999999998	19.855
55-59	40.32	33.14	4.835	21.705
60-64	46.67	10.665	32.625	10.040000000000001
65-69	21.17	0.295	39.839999999999996	38.695
70-74	15.705	5.305	0.165	78.825
75-79	31.06	47.165	18.34	3.435
80-84	11.325000000000001	38.46	18.545	31.669999999999998
85-89	25.435000000000002	20.7	21.085	32.78
90-94	23.635	26.07	23.13	27.165
95-99	15.190000000000001	25.195	27.42	32.195
100-104	21.13	24.72	27.134999999999998	27.015
105-109	18.61	30.81	24.985	25.595000000000002
110-114	19.84	31.6	21.57	26.99
115-119	27.345000000000002	31.125000000000004	22.935	18.595
120-124	23.65	35.245	16.495	24.610000000000003
125-129	26.840000000000003	23.565	24.055	25.540000000000003
130-134	32.11	20.565	22.425	24.9
135-139	26.655	19.15	24.865000000000002	29.330000000000002
140-144	30.64	19.53	28.265	21.565
145-149	33.915	18.67	23.815	23.599999999999998
150-154	38.455	13.675	27.279999999999998	20.59
155-159	33.879999999999995	20.9	29.294999999999998	15.925
160-164	42.57	15.8	26.674999999999997	14.954999999999998
165-169	37.485	14.510000000000002	33.845	14.16
170-174	34.985	16.875	34.735	13.405000000000001
175-179	40.315	15.305	32.565	11.815000000000001
180-184	30.205	16.03	34.285	19.48
185-189	28.79	14.979999999999999	35.765	20.465
190-194	31.495	16.31	30.259999999999998	21.935
195-199	26.424999999999997	14.215	30.930000000000003	28.43
200-204	21.855	13.525	38.4	26.22
205-209	22.13	16.34	34.54	26.99
210-214	20.32	23.119999999999997	32.57	23.990000000000002
215-219	16.45	20.455000000000002	42.72	20.375
220-224	22.58	24.115000000000002	33.650000000000006	19.655
225-229	14.245	32.2	33.51	20.044999999999998
230-234	13.450000000000001	22.23	37.285000000000004	27.034999999999997
235-239	12.97	32.795	27.155	27.08
240-244	11.41	32.105	27.560000000000002	28.925
245-249	13.270971937371817	27.497373818218197	28.242709219148615	30.988945025261366
250-251	4.910141206675225	24.71116816431322	30.32734274711168	40.05134788189987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.3333333333333333
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.3333333333333333
37	0.3333333333333333
38	0.0
39	0.0
40	0.0
41	0.3333333333333333
42	0.0
43	0.3333333333333333
44	0.3333333333333333
45	1.3333333333333333
46	3.666666666666667
47	12.000000000000004
48	26.99999999999997
49	59.33333333333348
50	99.333333333333
51	150.66666666666657
52	242.0000000000025
53	378.6666666666626
54	429.999999999993
55	343.33333333333127
56	182.0000000000008
57	51.33333333333342
58	17.000000000000007
59	6.333333333333331
60	3.0
61	2.333333333333333
62	1.9999999999999998
63	1.3333333333333333
64	1.3333333333333333
65	0.0
66	0.0
67	0.0
68	0.3333333333333333
69	0.0
70	0.0
71	0.0
72	0.6666666666666666
73	0.6666666666666666
74	0.3333333333333333
75	0.0
76	0.0
77	0.0
78	0.3333333333333333
79	0.3333333333333333
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
247	1.0
248	7.0
249	189.0
250	1374.0
251	2429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.44493392070484	16.3
2	5.726872246696035	3.25
3	4.140969162995595	3.5249999999999995
4	4.317180616740088	4.9
5	4.933920704845815	7.000000000000001
6	4.845814977973569	8.25
7	3.524229074889868	7.000000000000001
8	3.3480176211453743	7.6
9	2.2026431718061676	5.625
>10	9.515418502202644	36.55
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	34	0.8500000000000001	No Hit
TCTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	29	0.7250000000000001	No Hit
TTTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	27	0.675	No Hit
GTTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	27	0.675	No Hit
TTGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	24	0.6	No Hit
ATTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	23	0.575	No Hit
TTTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	22	0.5499999999999999	No Hit
TATTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	21	0.525	No Hit
GCTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	19	0.475	No Hit
TTATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	19	0.475	No Hit
ATTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	18	0.44999999999999996	No Hit
ATCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	18	0.44999999999999996	No Hit
TTGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	18	0.44999999999999996	No Hit
CTTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	18	0.44999999999999996	No Hit
TGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	18	0.44999999999999996	No Hit
TGGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	17	0.42500000000000004	No Hit
TTTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	17	0.42500000000000004	No Hit
TTACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	17	0.42500000000000004	No Hit
CCTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	17	0.42500000000000004	No Hit
TCGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	16	0.4	No Hit
TTTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	16	0.4	No Hit
GTGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	16	0.4	No Hit
TCTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
TCGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
GGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
TGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
ATATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
GTTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
TGTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	15	0.375	No Hit
GCTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
ATTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
GGTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
TCCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
CTGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
TAATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
ACATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
GTTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	14	0.35000000000000003	No Hit
GCACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
AGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
GGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
AATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
CGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TCCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TTGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TACCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TCTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	13	0.325	No Hit
CCTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TGGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
TTAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	13	0.325	No Hit
AGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
GCTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
GCGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
TGATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
CGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
TTGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	12	0.3	No Hit
CATCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
CGTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
CACTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
AATTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
AGATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
TCTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
CTTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
ATGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
TGGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	12	0.3	No Hit
CTTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	12	0.3	No Hit
CTTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
GTCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
TTCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
TGCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
TTCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
AACTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
TTTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	11	0.27499999999999997	No Hit
ATTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
ATGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
ATCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
CCGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
TGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
ACCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	11	0.27499999999999997	No Hit
CTTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	10	0.25	No Hit
TCATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TTCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	10	0.25	No Hit
CATTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TCGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	10	0.25	No Hit
CGGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GATTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
CATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
CTATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GCTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TTTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	10	0.25	No Hit
ATACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GGCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	10	0.25	No Hit
AGTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TACAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TGCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TTCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
ACGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GTAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
CTAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
CTTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	10	0.25	No Hit
GTTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TACTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GTGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GCCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
ACTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
GCATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TATCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	10	0.25	No Hit
TCTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
GATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
GGCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
CTACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
TTAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
ACGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
ATGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
GTATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
ATAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
TTTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
AGGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
GGGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
GTGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
ACTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
CCATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
CCGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
TTCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
AAATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
ACTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
CTCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
CTGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
GTGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
TAGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	9	0.22499999999999998	No Hit
ATTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
GTTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	9	0.22499999999999998	No Hit
CCTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TGCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
AGCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
GCGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
GTAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
GGAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
AGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
CTGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
CTCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
AAACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
CTGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TGCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TCCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
CGTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
AGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
CCTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
TCGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
GATAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
AAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
AATCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
GGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TAGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
CCTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
CCTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TCTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
GATCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
TATTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
ACGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
GTTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
CCCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
TAGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
ATGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
CCCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
CCGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
ACCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
GTTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	8	0.2	No Hit
CCCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	8	0.2	No Hit
ACTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
CAGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
AATAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
TACGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
ATACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TAAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GTATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
ATTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
GTGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TGAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
TCCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
CAATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
AATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TAACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
GGATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GGGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GTACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
TCGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
CGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
CAGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
CGGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TGAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
AGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
GGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TCGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GCGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GGGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TAAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
TAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
TAACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
CAACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
GTCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
CGCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
AGCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
ACGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GTGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GCCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
TATAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
GCCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	7	0.17500000000000002	No Hit
TGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	7	0.17500000000000002	No Hit
GTTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GAGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
ATGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
AGTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CTCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
GCCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
AACAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CATGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CGATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
TCAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
GGTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CAGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
ATCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TCACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CTACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
TTGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CACCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GGGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
AGTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
AACTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
AAGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CATAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
AATAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
AGGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CGGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GACTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CGGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
AACGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TTAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CTCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CGCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
GCCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TTGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TAATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
TGGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TACAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GGAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
AGCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
GATTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
TGCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GTACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
GCCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CCGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
ACTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
ATTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
AGAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TGGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
ACCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TTCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
CTGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	6	0.15	No Hit
CGAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	6	0.15	No Hit
TATCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GCTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GAATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
AAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
AGGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GAGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
GAGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
GCGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
CGCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CAACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
AGGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
CTATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TTAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CACGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GTAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CACAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TTGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TTTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CACGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
GGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TGCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
TCCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CTAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
GAATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TTCAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
ACATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
ATCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TCGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
AAAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
AAGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
GTCCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CGCGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
AGGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
CCAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
ACTGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CGAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GAGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TGATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TGCTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
CGAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
ACGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GGACGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
CGATGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
GCAAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
ACTAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TACCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GTGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
TGTCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
GTGCGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
CCAGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
ACGAGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGA	5	0.125	No Hit
CTGTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
ATTTGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
ATGGGAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGCAGAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.005	0.0
165-169	0.0	0.0	0.0	0.025	0.0
170-174	0.0	0.0	0.0	0.025	0.0
175-179	0.0	0.0	0.0	0.025	0.0
180-184	0.0	0.0	0.0	0.025	0.0
185-189	0.0	0.0	0.0	0.025	0.0
190-194	0.0	0.0	0.0	0.025	0.0
195-199	0.0	0.0	0.0	0.025	0.0
200-204	0.0	0.0	0.0	0.025	0.0
205-209	0.0	0.0	0.0	0.025	0.0
210-214	0.0	0.0	0.0	0.025	0.0
215-219	0.0	0.0	0.0	0.025	0.0
220-224	0.0	0.0	0.0	0.025	0.0
225-229	0.0	0.0	0.0	0.025	0.0
230-234	0.0	0.0	0.0	0.025	0.0
235-239	0.0	0.0	0.0	0.025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCACC	10	3.8509272E-4	376.32693	245
AGGCCCT	10	3.8509272E-4	376.32693	245
ATCACTC	10	3.8509272E-4	376.32693	245
ATATCTC	10	3.8509272E-4	376.32693	245
TGAGACG	110	0.0	244.6125	4
TTCGAGA	25	1.3478711E-9	244.6125	2
ATGAGAC	25	1.3478711E-9	244.6125	3
GTAGAGA	10	0.0014285702	244.61249	2
GGAGAGA	15	1.4179932E-5	244.61249	2
CAAGAGA	10	0.0014285702	244.61249	2
GTTGAGA	10	0.0014285702	244.61249	2
GGTGAGA	10	0.0014285702	244.61249	2
CTGAGAC	10	0.0014285702	244.61249	3
TCAGGAG	10	0.0014285702	244.61249	1
AAGAGAC	15	1.4179932E-5	244.61249	3
TATGAGA	10	0.0014285702	244.61249	2
TATTGAG	10	0.0014285702	244.61249	1
TTGAGAC	40	0.0	244.61249	3
TGGAGAC	30	1.2732926E-11	244.61249	3
GTGAGAC	35	0.0	244.61249	3
>>END_MODULE
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
Read 140746 spots for SRR32999354.sra
Written 140746 spots for SRR32999354.sra
Read 140745 spots for SRR32999354.sra
Written 140745 spots for SRR32999354.sra
SRR ids: ['SRR32999354.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7ilzunb9
SRR32999354.sra spots: 1125961
blocks: [[1, 140745], [140746, 281490], [281491, 422235], [422236, 562980], [562981, 703725], [703726, 844470], [844471, 985215], [985216, 1125961]]
SRR32999354 file size 640344
SRR32999354 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32999354 SRR32999354_1.fastq SRR32999354_2.fastq
Input file:	SRR32999354_1.fastq
Paired file:	SRR32999354_2.fastq
trimmed:	SRR32999354-trimmed-pair1.fastq, SRR32999354-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 17 17:49:39 2026 >> started

Tue Mar 17 17:49:41 2026 >> done (1.758s)
1125961 read pairs processed; of these:
      0 ( 0.00%) short read pairs filtered out after trimming by size control
      0 ( 0.00%) empty read pairs filtered out after trimming by size control
1125961 (100.00%) read pairs available; of these:
1125961 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
245	      1	  0.00%
246	      0	  0.00%
247	      5	  0.00%
248	    177	  0.02%
249	   5217	  0.46%
250	 499327	 44.35%
251	 621234	 55.17%
1125961 reads passed initial QC


criterion=sequence-density
sequence-density=0.01
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=1
prefix-density=0.00
prefix-fanout=1.0
sequence=GGGAGGCTTAGTGCAGCCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=1
prefix-density=0.00
prefix-fanout=1.0
sequence=GGGAGGCTTAGTGCAGCCT


criterion=sequence-density
sequence-density=0.18
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=19
prefix-density=0.01
prefix-fanout=1.0
sequence=GAGACGAGGGGGAAGACATTTGGGAAGGACTGACTCTCTGAGGAGACGGTGACCGTGGTCCCTGTGCCCCAGACATCGAAGTACCAGT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=22
fanout-score=17.95
fanout-score-rank=1
prefix-density=0.19
prefix-fanout=1.0
sequence=TGACTCTCTGCGGAGAC
SRR32999354 testing PE reads STAR mapping to Ensembl genome
Read2 map rate below 40%, dropping it and using Read1 only
                                 Started job on |	Mar 17 17:51:37
                             Started mapping on |	Mar 17 17:51:37
                                    Finished on |	Mar 17 17:51:40
       Mapping speed, Million of reads per hour |	1351.15

                          Number of input reads |	1125961
                      Average input read length |	230
                                    UNIQUE READS:
                   Uniquely mapped reads number |	1088049
                        Uniquely mapped reads % |	96.63%
                          Average mapped length |	230.59
                       Number of splices: Total |	8224
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	3750
                       Number of splices: GC/AG |	3682
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	790
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.04%
                        Deletion average length |	1.36
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	29146
             % of reads mapped to multiple loci |	2.59%
        Number of reads mapped to too many loci |	116
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.66%
                     % of reads unmapped: other |	0.11%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8766	8766	8766
N_multimapping	29146	29146	29146
N_noFeature	0	0	1087733
N_ambiguous	7506	7375	0
UnstrandedReadsAssigned:1080543 PositiveStrandReadsAssigned:1080674 NegativeStrandReadsAssigned:316
Dataset is classified positive stranded
MeadianReadLen=231 20thPercentileLength=231 echo kmer=227
SRR32999354 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in single-end mode
[quant] will process file 1: SRR32999354-trimmed-pair1.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 1,125,961 reads, 1,042,449 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,310 rounds

 109283 SRR32999354.ke.tsv
  52636 SRR32999354.se.tsv
 161919 total
==> SRR32999354.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR32999354.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR32999354 completed mapping pipeline successfully
