Starting /dee2/code/volunteer_pipeline.sh SRR33396256
    current disk space = 1076736156
    free memory = 198695368 
SRR33396256 SRAfilesize
ee882f63a45c126e5588b53b0e0ad6f5  SRR33396256.sra
SRR33396256.sra file validated
SRR33396256 is paired end
SRR33396256 is conventional basespace
SRR33396256 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR33396256_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.0395	35.0	34.0	37.0	32.0	38.0
2	34.7285	35.0	34.0	37.0	32.0	38.0
3	34.8425	35.0	34.0	37.0	32.0	38.0
4	34.581	35.0	34.0	37.0	31.0	38.0
5	34.57025	35.0	34.0	37.0	32.0	38.0
6	34.81225	35.0	34.0	37.0	32.0	38.0
7	34.76725	35.0	34.0	37.0	32.0	38.0
8	34.8005	35.0	34.0	37.0	32.0	38.0
9	34.85925	35.0	34.0	37.0	32.0	38.0
10-14	34.7733	35.0	34.0	37.0	32.2	38.0
15-19	34.66185	35.0	34.0	37.0	32.0	38.0
20-24	34.6248	35.0	34.0	37.0	32.0	38.0
25-29	34.63865	35.0	34.0	37.0	31.8	38.0
30-34	34.6931	35.0	34.0	37.0	32.0	38.0
35-39	34.73125	35.0	34.0	37.0	32.0	38.0
40-44	34.679500000000004	35.0	34.0	37.0	31.6	38.0
45-49	34.63825	35.0	34.0	37.0	31.8	38.0
50-54	34.5659	35.0	34.0	37.0	31.0	38.0
55-59	34.6005	35.2	34.0	37.0	31.4	38.2
60-64	34.557900000000004	35.0	34.0	37.0	31.0	38.0
65-69	34.43915	35.0	34.0	37.0	30.6	38.0
70-74	34.4792	35.0	34.0	37.0	31.0	38.0
75-79	34.41925	35.0	34.0	37.0	30.8	38.0
80-84	34.39765	35.0	34.0	37.0	30.6	38.0
85-89	34.437599999999996	35.0	34.0	37.0	30.8	38.0
90-94	34.255	35.0	34.0	37.0	30.0	38.0
95-99	34.217949999999995	35.0	34.0	37.0	30.0	38.0
100-104	34.217349999999996	35.0	34.0	37.0	30.0	38.0
105-109	34.0707	35.0	34.0	37.0	29.2	38.0
110-114	34.00435	35.0	34.0	37.0	29.0	38.0
115-119	33.8906	35.0	34.0	37.0	28.4	38.0
120-124	33.802800000000005	35.0	34.0	37.0	28.0	38.0
125-129	33.585249999999995	35.0	34.0	37.0	27.4	38.0
130-134	33.54885	35.0	33.8	37.0	27.0	38.0
135-139	33.43429999999999	35.0	33.4	37.0	26.8	38.0
140-144	33.294149999999995	35.0	33.2	37.0	26.2	38.0
145-149	33.36215	35.0	33.0	37.0	26.6	38.0
150	33.2025	35.0	33.0	37.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	2.0
26	18.0
27	32.0
28	73.0
29	109.0
30	144.0
31	199.0
32	246.0
33	434.0
34	769.0
35	1349.0
36	623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.925	11.95	14.45	45.675
2	21.275	20.325	35.3	23.1
3	25.25	21.349999999999998	26.0	27.400000000000002
4	27.750000000000004	30.225	19.3	22.725
5	26.924999999999997	31.574999999999996	22.1	19.400000000000002
6	21.875	32.275	21.075	24.775
7	19.325	16.8	39.300000000000004	24.575
8	20.625	19.0	28.925	31.45
9	22.275	20.225	30.925000000000004	26.575
10-14	22.435	26.840000000000003	26.005	24.72
15-19	24.085	25.729999999999997	27.395000000000003	22.79
20-24	25.169999999999998	27.839999999999996	25.91	21.08
25-29	23.695	27.97	26.21	22.125
30-34	23.59	27.29	25.669999999999998	23.45
35-39	22.925	26.619999999999997	25.979999999999997	24.474999999999998
40-44	23.695	27.084999999999997	25.430000000000003	23.79
45-49	24.265	26.295	25.855	23.585
50-54	24.115000000000002	27.495000000000005	24.355	24.035
55-59	24.55	25.974999999999998	23.74	25.735000000000003
60-64	23.72	26.384999999999998	24.975	24.92
65-69	25.235000000000003	26.6	24.759999999999998	23.405
70-74	23.24	27.42	25.724999999999998	23.615
75-79	23.75	24.985	27.200000000000003	24.065
80-84	24.295	24.975	27.834999999999997	22.895
85-89	23.265	25.89	27.21	23.635
90-94	24.59	24.425	27.655	23.330000000000002
95-99	24.195	25.22	27.250000000000004	23.335
100-104	22.975	24.6	27.794999999999998	24.63
105-109	25.035	24.22	27.29	23.455000000000002
110-114	24.84	25.88	24.575	24.705
115-119	24.474999999999998	25.445	24.875	25.205
120-124	24.565	27.08	23.95	24.404999999999998
125-129	24.04	28.000000000000004	23.29	24.67
130-134	24.5	27.97	23.419999999999998	24.11
135-139	23.82	28.62	23.03	24.529999999999998
140-144	23.895	28.29	22.795	25.019999999999996
145-149	22.485	28.48	22.275	26.76
150	22.925	26.25	22.975	27.85
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.5
20	0.5
21	0.0
22	1.0
23	1.5
24	1.5
25	2.5
26	7.5
27	9.5
28	11.0
29	15.5
30	16.5
31	20.0
32	26.0
33	36.5
34	41.5
35	41.5
36	39.0
37	34.5
38	32.0
39	34.0
40	36.0
41	33.5
42	33.5
43	54.0
44	131.5
45	197.5
46	257.0
47	327.5
48	369.5
49	368.5
50	337.5
51	305.5
52	271.0
53	230.5
54	169.5
55	129.0
56	142.0
57	107.5
58	42.0
59	26.5
60	19.0
61	16.0
62	11.0
63	5.0
64	1.0
65	0.5
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.5
73	0.5
74	0.0
75	0.5
76	0.5
77	0.0
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4245171462357	50.375
2	10.327158060701617	13.100000000000001
3	3.90224674812771	7.425
4	1.931415057154119	4.9
5	1.2219156484036262	3.875
6	0.7883326763894364	3.0
7	0.7883326763894364	3.5000000000000004
8	0.31533307055577453	1.6
9	0.3941663381947182	2.25
>10	0.9065825778478518	9.975000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA	33	0.8250000000000001	No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT	25	0.625	No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG	25	0.625	No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT	24	0.6	No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG	24	0.6	No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	23	0.575	No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT	23	0.575	No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA	23	0.575	No Hit
CAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGC	18	0.44999999999999996	No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC	16	0.4	No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA	16	0.4	No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC	15	0.375	No Hit
GTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCAC	14	0.35000000000000003	No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC	13	0.325	No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC	13	0.325	No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC	13	0.325	No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT	13	0.325	No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC	12	0.3	No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG	12	0.3	No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACT	12	0.3	No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA	12	0.3	No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG	10	0.25	No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT	10	0.25	No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAA	9	0.22499999999999998	No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT	9	0.22499999999999998	No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA	9	0.22499999999999998	No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	9	0.22499999999999998	No Hit
TTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACG	9	0.22499999999999998	No Hit
CCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACG	9	0.22499999999999998	No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCA	9	0.22499999999999998	No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG	9	0.22499999999999998	No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA	9	0.22499999999999998	No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA	9	0.22499999999999998	No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	8	0.2	No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA	8	0.2	No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG	8	0.2	No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATC	8	0.2	No Hit
CCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGC	8	0.2	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA	8	0.2	No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA	8	0.2	No Hit
CGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCA	8	0.2	No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC	7	0.17500000000000002	No Hit
CCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGA	7	0.17500000000000002	No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC	7	0.17500000000000002	No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG	7	0.17500000000000002	No Hit
GCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAG	7	0.17500000000000002	No Hit
CCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTC	7	0.17500000000000002	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT	7	0.17500000000000002	No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA	7	0.17500000000000002	No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA	7	0.17500000000000002	No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA	7	0.17500000000000002	No Hit
AATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCA	7	0.17500000000000002	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG	7	0.17500000000000002	No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC	7	0.17500000000000002	No Hit
TTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGG	7	0.17500000000000002	No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA	7	0.17500000000000002	No Hit
CGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAG	7	0.17500000000000002	No Hit
GAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGG	7	0.17500000000000002	No Hit
TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCG	7	0.17500000000000002	No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC	7	0.17500000000000002	No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA	7	0.17500000000000002	No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA	6	0.15	No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA	6	0.15	No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC	6	0.15	No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG	6	0.15	No Hit
TTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGA	6	0.15	No Hit
GCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCT	6	0.15	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	6	0.15	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC	6	0.15	No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAA	6	0.15	No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT	6	0.15	No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAG	6	0.15	No Hit
CCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT	6	0.15	No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC	6	0.15	No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG	6	0.15	No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG	6	0.15	No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC	6	0.15	No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG	6	0.15	No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA	6	0.15	No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA	6	0.15	No Hit
AAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCT	6	0.15	No Hit
GAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTC	5	0.125	No Hit
CCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTA	5	0.125	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG	5	0.125	No Hit
GGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAG	5	0.125	No Hit
CTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAAT	5	0.125	No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA	5	0.125	No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC	5	0.125	No Hit
ATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTG	5	0.125	No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA	5	0.125	No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC	5	0.125	No Hit
CGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGA	5	0.125	No Hit
TTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCAT	5	0.125	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG	5	0.125	No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT	5	0.125	No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA	5	0.125	No Hit
CGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCC	5	0.125	No Hit
GAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTA	5	0.125	No Hit
CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT	5	0.125	No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC	5	0.125	No Hit
AGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA	5	0.125	No Hit
GGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGA	5	0.125	No Hit
TAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAA	5	0.125	No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA	5	0.125	No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGT	5	0.125	No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC	5	0.125	No Hit
CTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGA	5	0.125	No Hit
TGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTG	5	0.125	No Hit
ATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGG	5	0.125	No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT	5	0.125	No Hit
CTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTAC	5	0.125	No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.025	0.0	0.0	0.0	0.0
42-43	0.037500000000000006	0.0	0.0	0.0	0.0
44-45	0.05	0.0	0.0	0.0	0.0
46-47	0.05	0.0	0.0	0.0	0.0
48-49	0.05	0.0	0.0	0.0	0.0
50-51	0.075	0.0	0.0	0.0	0.0
52-53	0.075	0.0	0.0	0.0	0.0
54-55	0.075	0.0	0.0	0.0	0.0
56-57	0.075	0.0	0.0	0.0	0.0
58-59	0.075	0.0	0.0	0.0	0.0
60-61	0.1	0.0	0.0	0.0	0.0
62-63	0.125	0.0	0.0	0.0	0.0
64-65	0.125	0.0	0.0	0.0	0.0
66-67	0.15	0.0	0.0	0.0	0.0
68-69	0.15	0.0	0.0	0.0	0.0
70-71	0.225	0.0	0.0	0.0	0.0
72-73	0.2875	0.0	0.0	0.0	0.0
74-75	0.32499999999999996	0.0	0.0	0.0	0.0
76-77	0.375	0.0	0.0	0.0	0.0
78-79	0.4125	0.0	0.0	0.0	0.0
80-81	0.5625	0.0	0.0	0.0	0.0
82-83	0.7	0.0	0.0	0.0	0.0
84-85	0.9375	0.0	0.0	0.0	0.0
86-87	1.0625	0.0	0.0	0.0	0.0
88-89	1.1875	0.0	0.0	0.0	0.0
90-91	1.3624999999999998	0.0	0.0	0.0	0.0
92-93	1.6	0.0	0.0	0.0	0.0
94-95	1.9500000000000002	0.0	0.0	0.0	0.0
96-97	2.5374999999999996	0.0	0.0	0.0	0.0
98-99	3.0	0.0	0.0	0.0	0.0
100-101	3.7125	0.0	0.0	0.0	0.0
102-103	4.6	0.0	0.0	0.0	0.0
104-105	5.85	0.0	0.0	0.0	0.0
106-107	6.775	0.0	0.0	0.0	0.0
108-109	7.6	0.0	0.0	0.0	0.0
110-111	8.575	0.0	0.0	0.0	0.0
112-113	9.787500000000001	0.0	0.0	0.0	0.0
114-115	11.525	0.0	0.0	0.0	0.0
116-117	12.95	0.0	0.0	0.0	0.0
118-119	14.6375	0.0	0.0	0.0	0.0
120-121	16.05	0.0	0.0	0.0	0.0
122-123	17.612499999999997	0.0	0.0	0.0	0.0
124-125	19.4625	0.0	0.0	0.0	0.0
126-127	21.525	0.0	0.0	0.0	0.0
128-129	23.4	0.0	0.0	0.0	0.0
130-131	25.2875	0.0	0.0	0.0	0.0
132-133	27.3625	0.0	0.0	0.0	0.0
134-135	30.225	0.0	0.0	0.0	0.0
136-137	32.925	0.0	0.0	0.0	0.0
138	34.55	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCAT	20	3.687869E-4	108.0	7
GTCGGCA	20	3.687869E-4	108.0	6
AGAGCAC	115	0.002704152	10.01739	140-144
TCGGAAG	130	0.007441913	8.861538	135-139
>>END_MODULE
SRR33396256 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR33396256_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0515	37.0	35.0	38.0	34.0	39.0
2	35.56875	36.0	34.0	37.0	33.0	39.0
3	35.68825	36.0	35.0	38.0	33.0	39.0
4	35.61475	36.0	35.0	38.0	33.0	39.0
5	35.44825	36.0	35.0	37.0	33.0	39.0
6	35.6065	36.0	35.0	37.0	33.0	39.0
7	35.403	35.0	34.0	37.0	33.0	39.0
8	35.52725	36.0	34.0	37.0	33.0	39.0
9	35.60425	36.0	34.0	37.0	33.0	39.0
10-14	35.418899999999994	36.0	34.2	37.0	33.0	39.0
15-19	35.35635	36.0	34.0	37.0	33.0	39.0
20-24	35.321200000000005	36.0	34.0	37.0	32.8	39.0
25-29	35.2139	36.0	34.0	37.0	33.0	39.0
30-34	35.2561	36.0	34.0	37.0	32.8	39.0
35-39	35.09325	36.0	34.0	37.0	32.2	39.0
40-44	35.00725	36.0	34.0	37.0	32.0	39.0
45-49	34.96325	35.8	34.0	37.0	32.0	39.0
50-54	34.78435	35.4	34.0	37.0	31.4	39.0
55-59	34.650099999999995	35.4	34.0	37.0	30.8	39.0
60-64	34.544799999999995	35.2	34.0	37.0	30.2	39.0
65-69	34.5215	35.0	34.0	37.0	30.2	39.0
70-74	34.28305	35.0	34.0	37.0	29.8	39.0
75-79	34.27865	35.0	34.0	37.0	29.8	39.0
80-84	34.043000000000006	35.0	34.0	37.0	29.0	38.6
85-89	33.830349999999996	35.0	33.6	37.0	28.0	38.8
90-94	33.732600000000005	35.0	33.2	37.0	27.8	38.6
95-99	33.39375	35.0	33.0	37.0	26.4	38.4
100-104	33.37755	35.0	33.0	37.0	26.6	38.0
105-109	33.02905	35.0	33.0	37.0	25.4	38.0
110-114	32.755	35.0	32.4	37.0	24.0	38.0
115-119	32.4537	35.0	31.8	37.0	22.8	38.0
120-124	32.1768	34.6	31.6	36.4	22.0	38.0
125-129	32.019349999999996	34.4	31.4	36.0	21.8	38.0
130-134	31.60375	34.0	30.2	36.0	19.8	38.0
135-139	31.4146	34.0	30.2	36.0	19.2	38.0
140-144	30.701999999999998	34.0	28.8	36.0	17.2	38.0
145-149	30.263800000000003	34.0	27.6	36.0	15.6	38.0
150	30.01275	34.0	27.0	36.0	15.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	3.0
23	4.0
24	10.0
25	33.0
26	48.0
27	73.0
28	93.0
29	119.0
30	180.0
31	249.0
32	343.0
33	465.0
34	740.0
35	987.0
36	653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.875	10.825	13.775	47.525
2	22.775000000000002	19.650000000000002	33.375	24.2
3	24.95	21.25	24.275	29.525000000000002
4	28.275	29.475	17.375	24.875
5	28.9	30.25	20.75	20.1
6	22.5	33.025	20.75	23.724999999999998
7	19.55	16.975	38.625	24.85
8	19.900000000000002	19.975	28.075	32.05
9	24.349999999999998	21.099999999999998	29.2	25.35
10-14	22.875	26.745	25.4	24.98
15-19	23.23	26.419999999999998	27.35	23.0
20-24	24.884999999999998	27.485	25.645	21.985
25-29	23.315	27.750000000000004	26.125	22.81
30-34	22.835	27.955000000000002	24.705	24.505
35-39	22.545	27.145000000000003	25.665	24.645
40-44	23.055	27.200000000000003	25.305	24.44
45-49	24.085	26.634999999999998	24.785	24.495
50-54	24.635	27.485	23.905	23.974999999999998
55-59	24.37	26.47	23.745	25.415
60-64	24.224999999999998	27.689999999999998	23.84	24.245
65-69	24.595	27.51	24.12	23.775
70-74	23.09	27.82	24.805	24.285
75-79	23.74	25.94	26.97	23.35
80-84	23.39	25.66	27.58	23.369999999999997
85-89	22.705000000000002	26.895000000000003	26.845000000000002	23.555
90-94	24.43	25.09	26.705000000000002	23.775
95-99	23.875	25.355	27.375	23.395
100-104	22.82	25.619999999999997	27.215	24.345
105-109	24.975	24.86	26.82	23.345
110-114	25.515	26.040000000000003	24.985	23.46
115-119	26.365	26.235000000000003	24.42	22.98
120-124	26.08	26.995	24.235	22.689999999999998
125-129	27.415	27.355	23.580000000000002	21.65
130-134	28.34	27.37	24.13	20.16
135-139	28.975	28.000000000000004	23.655	19.37
140-144	29.89	27.894999999999996	23.405	18.81
145-149	31.064999999999998	26.695	23.145	19.095000000000002
150	31.574999999999996	25.1	23.35	19.975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.0
24	1.5
25	2.5
26	4.5
27	14.5
28	21.0
29	17.0
30	20.0
31	22.0
32	24.0
33	29.5
34	26.0
35	29.5
36	40.5
37	43.0
38	36.0
39	31.5
40	32.5
41	28.5
42	35.0
43	65.0
44	143.0
45	205.0
46	244.0
47	312.5
48	375.5
49	367.0
50	307.5
51	272.5
52	264.0
53	239.5
54	181.0
55	140.5
56	151.5
57	117.0
58	54.5
59	38.5
60	22.5
61	17.5
62	11.5
63	3.0
64	2.0
65	1.5
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.5
72	1.0
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55742532604123	44.9
2	12.284392090870845	14.6
3	5.090450147244425	9.075
4	2.187631468237274	5.2
5	1.1779554059739168	3.5000000000000004
6	0.9676062263357174	3.45
7	0.5469078670593185	2.275
8	0.37862852334875896	1.7999999999999998
9	0.5048380311316786	2.7
>10	1.3041649137568363	12.5
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA	29	0.7250000000000001	No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG	28	0.7000000000000001	No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA	24	0.6	No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG	23	0.575	No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC	22	0.5499999999999999	No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC	21	0.525	No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT	21	0.525	No Hit
CAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGC	20	0.5	No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	20	0.5	No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC	19	0.475	No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA	19	0.475	No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA	18	0.44999999999999996	No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT	17	0.42500000000000004	No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC	16	0.4	No Hit
GTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCAC	16	0.4	No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC	16	0.4	No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA	14	0.35000000000000003	No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT	14	0.35000000000000003	No Hit
CCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACG	13	0.325	No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGC	12	0.3	No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC	12	0.3	No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA	12	0.3	No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG	11	0.27499999999999997	No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG	11	0.27499999999999997	No Hit
CCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGC	11	0.27499999999999997	No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG	11	0.27499999999999997	No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG	10	0.25	No Hit
GCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAG	10	0.25	No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT	10	0.25	No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA	10	0.25	No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA	10	0.25	No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA	9	0.22499999999999998	No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG	9	0.22499999999999998	No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA	9	0.22499999999999998	No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA	9	0.22499999999999998	No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG	9	0.22499999999999998	No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG	9	0.22499999999999998	No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG	9	0.22499999999999998	No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA	9	0.22499999999999998	No Hit
TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCG	9	0.22499999999999998	No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC	9	0.22499999999999998	No Hit
TATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG	9	0.22499999999999998	No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT	9	0.22499999999999998	No Hit
CATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCC	8	0.2	No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	8	0.2	No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA	8	0.2	No Hit
CAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCT	8	0.2	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG	8	0.2	No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA	8	0.2	No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT	8	0.2	No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG	8	0.2	No Hit
CTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACG	8	0.2	No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT	7	0.17500000000000002	No Hit
GCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCT	7	0.17500000000000002	No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	7	0.17500000000000002	No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAG	7	0.17500000000000002	No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC	7	0.17500000000000002	No Hit
CGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT	7	0.17500000000000002	No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC	7	0.17500000000000002	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	7	0.17500000000000002	No Hit
ACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGC	7	0.17500000000000002	No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG	7	0.17500000000000002	No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC	7	0.17500000000000002	No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG	7	0.17500000000000002	No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT	7	0.17500000000000002	No Hit
CTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCC	6	0.15	No Hit
CGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGC	6	0.15	No Hit
GCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTG	6	0.15	No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG	6	0.15	No Hit
TTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCC	6	0.15	No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC	6	0.15	No Hit
GTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGAC	6	0.15	No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT	6	0.15	No Hit
CGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG	6	0.15	No Hit
TTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGG	6	0.15	No Hit
CATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGA	6	0.15	No Hit
CGGTGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAA	6	0.15	No Hit
CGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC	6	0.15	No Hit
CGACAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGT	6	0.15	No Hit
AAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCG	6	0.15	No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG	6	0.15	No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC	6	0.15	No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC	6	0.15	No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT	6	0.15	No Hit
GTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCG	6	0.15	No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT	6	0.15	No Hit
CGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCA	6	0.15	No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG	6	0.15	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG	5	0.125	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	5	0.125	No Hit
GAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACA	5	0.125	No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA	5	0.125	No Hit
TACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAA	5	0.125	No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA	5	0.125	No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC	5	0.125	No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAA	5	0.125	No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA	5	0.125	No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC	5	0.125	No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT	5	0.125	No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA	5	0.125	No Hit
TATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGG	5	0.125	No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC	5	0.125	No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACT	5	0.125	No Hit
CGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATA	5	0.125	No Hit
CAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACT	5	0.125	No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC	5	0.125	No Hit
TAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCA	5	0.125	No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA	5	0.125	No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC	5	0.125	No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTA	5	0.125	No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTAC	5	0.125	No Hit
GTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGA	5	0.125	No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA	5	0.125	No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTT	5	0.125	No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC	5	0.125	No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.025	0.0	0.0	0.0	0.0
42-43	0.037500000000000006	0.0	0.0	0.0	0.0
44-45	0.05	0.0	0.0	0.0	0.0
46-47	0.05	0.0	0.0	0.0	0.0
48-49	0.05	0.0	0.0	0.0	0.0
50-51	0.075	0.0	0.0	0.0	0.0
52-53	0.075	0.0	0.0	0.0	0.0
54-55	0.075	0.0	0.0	0.0	0.0
56-57	0.075	0.0	0.0	0.0	0.0
58-59	0.075	0.0	0.0	0.0	0.0
60-61	0.1	0.0	0.0	0.0	0.0
62-63	0.125	0.0	0.0	0.0	0.0
64-65	0.125	0.0	0.0	0.0	0.0
66-67	0.15	0.0	0.0	0.0	0.0
68-69	0.15	0.0	0.0	0.0	0.0
70-71	0.21250000000000002	0.0	0.0	0.0	0.0
72-73	0.2625	0.0	0.0	0.0	0.0
74-75	0.3125	0.0	0.0	0.0	0.0
76-77	0.375	0.0	0.0	0.0	0.0
78-79	0.4125	0.0	0.0	0.0	0.0
80-81	0.575	0.0	0.0	0.0	0.0
82-83	0.7250000000000001	0.0	0.0	0.0	0.0
84-85	0.9625	0.0	0.0	0.0	0.0
86-87	1.0750000000000002	0.0	0.0	0.0	0.0
88-89	1.2375	0.0	0.0	0.0	0.0
90-91	1.4125	0.0	0.0	0.0	0.0
92-93	1.625	0.0	0.0	0.0	0.0
94-95	1.9500000000000002	0.0	0.0	0.0	0.0
96-97	2.475	0.0	0.0	0.0	0.0
98-99	2.9625	0.0	0.0	0.0	0.0
100-101	3.6875	0.0	0.0	0.0	0.0
102-103	4.574999999999999	0.0	0.0	0.0	0.0
104-105	5.7875	0.0	0.0	0.0	0.0
106-107	6.7125	0.0	0.0	0.0	0.0
108-109	7.5375	0.0	0.0	0.0	0.0
110-111	8.524999999999999	0.0	0.0	0.0	0.0
112-113	9.8125	0.0	0.0	0.0	0.0
114-115	11.524999999999999	0.0	0.0	0.0	0.0
116-117	12.7875	0.0	0.0	0.0	0.0
118-119	14.399999999999999	0.0	0.0	0.0	0.0
120-121	15.875	0.0	0.0	0.0	0.0
122-123	17.35	0.0	0.0	0.0	0.0
124-125	19.075000000000003	0.0	0.0	0.0	0.0
126-127	21.1125	0.0	0.0	0.0	0.0
128-129	23.0	0.0	0.0	0.0	0.0
130-131	24.775	0.0	0.0	0.0	0.0
132-133	26.775	0.0	0.0	0.0	0.0
134-135	29.5625	0.0	0.0	0.0	0.0
136-137	32.175	0.0	0.0	0.0	0.0
138	33.675	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGCGT	115	0.002704152	10.01739	140-144
>>END_MODULE
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
Read 1450758 spots for SRR33396256.sra
Written 1450758 spots for SRR33396256.sra
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
Read 1450754 spots for SRR33396256.sra
Written 1450754 spots for SRR33396256.sra
SRR ids: ['SRR33396256.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_iw6xvn94
SRR33396256.sra spots: 11606036
blocks: [[1, 1450754], [1450755, 2901508], [2901509, 4352262], [4352263, 5803016], [5803017, 7253770], [7253771, 8704524], [8704525, 10155278], [10155279, 11606036]]
SRR33396256 file size 4137408
SRR33396256 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR33396256 SRR33396256_1.fastq SRR33396256_2.fastq
Input file:	SRR33396256_1.fastq
Paired file:	SRR33396256_2.fastq
trimmed:	SRR33396256-trimmed-pair1.fastq, SRR33396256-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 10 01:58:37 2026 >> started

Tue Mar 10 01:59:02 2026 >> done (25.193s)
11606036 read pairs processed; of these:
     321 ( 0.00%) short read pairs filtered out after trimming by size control
     225 ( 0.00%) empty read pairs filtered out after trimming by size control
11605490 (100.00%) read pairs available; of these:
 6343736 (54.66%) trimmed read pairs available after processing
 5261754 (45.34%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     132	  0.00%
 19	      54	  0.00%
 20	      41	  0.00%
 21	      76	  0.00%
 22	      60	  0.00%
 23	      52	  0.00%
 24	      54	  0.00%
 25	      38	  0.00%
 26	      41	  0.00%
 27	      41	  0.00%
 28	      58	  0.00%
 29	      54	  0.00%
 30	      57	  0.00%
 31	      31	  0.00%
 32	      45	  0.00%
 33	      51	  0.00%
 34	      54	  0.00%
 35	     146	  0.00%
 36	      49	  0.00%
 37	     101	  0.00%
 38	      79	  0.00%
 39	     158	  0.00%
 40	     100	  0.00%
 41	     118	  0.00%
 42	      73	  0.00%
 43	     123	  0.00%
 44	      65	  0.00%
 45	      99	  0.00%
 46	      98	  0.00%
 47	     125	  0.00%
 48	     154	  0.00%
 49	     180	  0.00%
 50	     189	  0.00%
 51	     186	  0.00%
 52	     196	  0.00%
 53	     272	  0.00%
 54	     207	  0.00%
 55	     269	  0.00%
 56	     295	  0.00%
 57	     500	  0.00%
 58	     456	  0.00%
 59	     535	  0.00%
 60	     576	  0.00%
 61	     732	  0.01%
 62	     770	  0.01%
 63	     812	  0.01%
 64	     740	  0.01%
 65	     786	  0.01%
 66	     920	  0.01%
 67	    1138	  0.01%
 68	    1406	  0.01%
 69	    1670	  0.01%
 70	    1904	  0.02%
 71	    2284	  0.02%
 72	    2636	  0.02%
 73	    2579	  0.02%
 74	    2743	  0.02%
 75	    2823	  0.02%
 76	    3111	  0.03%
 77	    3457	  0.03%
 78	    4135	  0.04%
 79	    5366	  0.05%
 80	    6015	  0.05%
 81	    6802	  0.06%
 82	    7749	  0.07%
 83	    8273	  0.07%
 84	    9816	  0.08%
 85	   10483	  0.09%
 86	   10221	  0.09%
 87	   11128	  0.10%
 88	   12151	  0.10%
 89	   14514	  0.13%
 90	   16578	  0.14%
 91	   20180	  0.17%
 92	   23903	  0.21%
 93	   26190	  0.23%
 94	   31237	  0.27%
 95	   31646	  0.27%
 96	   32708	  0.28%
 97	   30744	  0.26%
 98	   34290	  0.30%
 99	   39469	  0.34%
100	   45375	  0.39%
101	   53076	  0.46%
102	   54952	  0.47%
103	   61011	  0.53%
104	   63630	  0.55%
105	   63804	  0.55%
106	   65175	  0.56%
107	   61530	  0.53%
108	   67038	  0.58%
109	   70926	  0.61%
110	   80739	  0.70%
111	   93229	  0.80%
112	   99499	  0.86%
113	  114571	  0.99%
114	  103301	  0.89%
115	  108184	  0.93%
116	  112505	  0.97%
117	  113870	  0.98%
118	   94773	  0.82%
119	  100978	  0.87%
120	  104462	  0.90%
121	  116766	  1.01%
122	  127226	  1.10%
123	  134045	  1.16%
124	  150082	  1.29%
125	  127821	  1.10%
126	  134638	  1.16%
127	  134265	  1.16%
128	  120826	  1.04%
129	  145083	  1.25%
130	  117914	  1.02%
131	  136691	  1.18%
132	  130282	  1.12%
133	  148365	  1.28%
134	  176651	  1.52%
135	  171187	  1.48%
136	  156609	  1.35%
137	  131847	  1.14%
138	  130257	  1.12%
139	  125013	  1.08%
140	  135287	  1.17%
141	  133992	  1.15%
142	  125344	  1.08%
143	  141613	  1.22%
144	  163852	  1.41%
145	  141372	  1.22%
146	  142097	  1.22%
147	  124416	  1.07%
148	  135639	  1.17%
149	  321531	  2.77%
150	 5261754	 45.34%
11605490 reads passed initial QC


criterion=sequence-density
sequence-density=7.81
sequence-density-rank=1
fanout-score=2.08
fanout-score-rank=21
prefix-density=8.14
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.12
sequence-density-rank=22
fanout-score=16.58
fanout-score-rank=1
prefix-density=1.67
prefix-fanout=1.2
sequence=CAAGCTCAACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATCAGACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA


criterion=sequence-density
sequence-density=8.02
sequence-density-rank=1
fanout-score=2.07
fanout-score-rank=25
prefix-density=8.32
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=294.47
fanout-score-rank=1
prefix-density=1.58
prefix-fanout=1.0
sequence=CCGGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCTCCCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGT
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AGCGACGTCGCT -y AGCGACGTCGCT -o SRR33396256 SRR33396256_1.fastq SRR33396256_2.fastq
Input file:	SRR33396256_1.fastq
Paired file:	SRR33396256_2.fastq
trimmed:	SRR33396256-trimmed-pair1.fastq, SRR33396256-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGCGACGTCGCT
-- paired 3' end adapter sequence (-y):	AGCGACGTCGCT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Tue Mar 10 01:59:36 2026 >> started

Tue Mar 10 01:59:45 2026 >> done (8.423s)
9026492 read pairs processed; of these:
   1119 ( 0.01%) short read pairs filtered out after trimming by size control
   8464 ( 0.09%) empty read pairs filtered out after trimming by size control
9016909 (99.89%) read pairs available; of these:
    431 ( 0.00%) trimmed read pairs available after processing
9016478 (100.00%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	    101	  0.00%
 19	     44	  0.00%
 20	     35	  0.00%
 21	     59	  0.00%
 22	     51	  0.00%
 23	     40	  0.00%
 24	     45	  0.00%
 25	     31	  0.00%
 26	     33	  0.00%
 27	     32	  0.00%
 28	     51	  0.00%
 29	     43	  0.00%
 30	     43	  0.00%
 31	     26	  0.00%
 32	     35	  0.00%
 33	     45	  0.00%
 34	     46	  0.00%
 35	    122	  0.00%
 36	     41	  0.00%
 37	     80	  0.00%
 38	     59	  0.00%
 39	    120	  0.00%
 40	     72	  0.00%
 41	     97	  0.00%
 42	     60	  0.00%
 43	     97	  0.00%
 44	     49	  0.00%
 45	     68	  0.00%
 46	     78	  0.00%
 47	     86	  0.00%
 48	    124	  0.00%
 49	    148	  0.00%
 50	    142	  0.00%
 51	    142	  0.00%
 52	    153	  0.00%
 53	    204	  0.00%
 54	    164	  0.00%
 55	    211	  0.00%
 56	    234	  0.00%
 57	    390	  0.00%
 58	    370	  0.00%
 59	    408	  0.00%
 60	    436	  0.00%
 61	    585	  0.01%
 62	    589	  0.01%
 63	    616	  0.01%
 64	    575	  0.01%
 65	    605	  0.01%
 66	    711	  0.01%
 67	    900	  0.01%
 68	   1086	  0.01%
 69	   1345	  0.01%
 70	   1477	  0.02%
 71	   1777	  0.02%
 72	   2047	  0.02%
 73	   1995	  0.02%
 74	   2107	  0.02%
 75	   2194	  0.02%
 76	   2411	  0.03%
 77	   2617	  0.03%
 78	   3222	  0.04%
 79	   4169	  0.05%
 80	   4642	  0.05%
 81	   5253	  0.06%
 82	   6020	  0.07%
 83	   6425	  0.07%
 84	   7610	  0.08%
 85	   8127	  0.09%
 86	   7987	  0.09%
 87	   8617	  0.10%
 88	   9558	  0.11%
 89	  11295	  0.13%
 90	  12809	  0.14%
 91	  15674	  0.17%
 92	  18618	  0.21%
 93	  20395	  0.23%
 94	  24330	  0.27%
 95	  24559	  0.27%
 96	  25485	  0.28%
 97	  23972	  0.27%
 98	  26653	  0.30%
 99	  30575	  0.34%
100	  35261	  0.39%
101	  41016	  0.45%
102	  42827	  0.47%
103	  47362	  0.53%
104	  49570	  0.55%
105	  49554	  0.55%
106	  50446	  0.56%
107	  47830	  0.53%
108	  52019	  0.58%
109	  55207	  0.61%
110	  62959	  0.70%
111	  72548	  0.80%
112	  77275	  0.86%
113	  89294	  0.99%
114	  80016	  0.89%
115	  84254	  0.93%
116	  87533	  0.97%
117	  88643	  0.98%
118	  73716	  0.82%
119	  78587	  0.87%
120	  81046	  0.90%
121	  91012	  1.01%
122	  99040	  1.10%
123	 104286	  1.16%
124	 116728	  1.29%
125	  99665	  1.11%
126	 104758	  1.16%
127	 104404	  1.16%
128	  94166	  1.04%
129	 112742	  1.25%
130	  91765	  1.02%
131	 106388	  1.18%
132	 101273	  1.12%
133	 115401	  1.28%
134	 137154	  1.52%
135	 133029	  1.48%
136	 121748	  1.35%
137	 102211	  1.13%
138	 100704	  1.12%
139	  97314	  1.08%
140	 105265	  1.17%
141	 104094	  1.15%
142	  97609	  1.08%
143	 110129	  1.22%
144	 127024	  1.41%
145	 109772	  1.22%
146	 110731	  1.23%
147	  96910	  1.07%
148	 105699	  1.17%
149	 249522	  2.77%
150	4084856	 45.30%


criterion=sequence-density
sequence-density=7.76
sequence-density-rank=1
fanout-score=2.09
fanout-score-rank=23
prefix-density=8.13
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=45
fanout-score=276.18
fanout-score-rank=1
prefix-density=1.36
prefix-fanout=1.0
sequence=ACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTACGTCGGGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTC


criterion=sequence-density
sequence-density=7.95
sequence-density-rank=1
fanout-score=2.08
fanout-score-rank=22
prefix-density=8.31
prefix-fanout=2.0
sequence=AGCGACGTCGCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=42
fanout-score=25.88
fanout-score-rank=1
prefix-density=0.13
prefix-fanout=1.0
sequence=CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGCTGGGTCGGTCGGGCTGGG
SRR33396256 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 10 02:00:43
                             Started mapping on |	Mar 10 02:00:43
                                    Finished on |	Mar 10 02:01:45
       Mapping speed, Million of reads per hour |	673.31

                          Number of input reads |	11595907
                      Average input read length |	273
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10052997
                        Uniquely mapped reads % |	86.69%
                          Average mapped length |	271.32
                       Number of splices: Total |	585799
            Number of splices: Annotated (sjdb) |	570413
                       Number of splices: GT/AG |	568632
                       Number of splices: GC/AG |	7341
                       Number of splices: AT/AC |	561
               Number of splices: Non-canonical |	9265
                      Mismatch rate per base, % |	0.76%
                         Deletion rate per base |	0.02%
                        Deletion average length |	3.59
                        Insertion rate per base |	0.03%
                       Insertion average length |	2.42
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	932645
             % of reads mapped to multiple loci |	8.04%
        Number of reads mapped to too many loci |	40554
             % of reads mapped to too many loci |	0.35%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.71%
                     % of reads unmapped: other |	0.20%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	610265	610265	610265
N_multimapping	932645	932645	932645
N_noFeature	3426013	5658893	5630899
N_ambiguous	2252436	796515	906932
UnstrandedReadsAssigned:4374548 PositiveStrandReadsAssigned:3597589 NegativeStrandReadsAssigned:3515166
Dataset is classified unstranded
MeadianReadLen=147 20thPercentileLength=122 echo kmer=117
SRR33396256 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR33396256-trimmed-pair1.fastq
                             SRR33396256-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 11,595,907 reads, 3,859,494 reads pseudoaligned
[quant] estimated average fragment length: 153.916
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 890 rounds

 109283 SRR33396256.ke.tsv
  52636 SRR33396256.se.tsv
 161919 total
==> SRR33396256.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	8.16667	0	0
ENSMUST00000196221.1	9	2.6	0	0
ENSMUST00000179664.1	11	4.6	0	0
ENSMUST00000178537.1	12	5.6	0	0
ENSMUST00000178862.1	14	7.6	0	0
ENSMUST00000179520.1	11	4.6	0	0
ENSMUST00000179883.1	16	8.16667	0	0
ENSMUST00000195858.1	10	3.6	0	0
ENSMUST00000179932.1	12	5.6	0	0

==> SRR33396256.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	0
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR33396256 completed mapping pipeline successfully
