Starting /dee2/code/volunteer_pipeline.sh SRR33623191
    current disk space = 1223004996
    free memory = 201266420 
SRR33623191 SRAfilesize
d2ebc9b8810541732804894b810686f6  SRR33623191.sra
SRR33623191.sra file validated
SRR33623191 is paired end
SRR33623191 is conventional basespace
SRR33623191 read1 length is 102-149 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR33623191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	102-149
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.15925	37.0	37.0	37.0	37.0	37.0
2	36.17875	37.0	37.0	37.0	37.0	37.0
3	36.24375	37.0	37.0	37.0	37.0	37.0
4	36.18875	37.0	37.0	37.0	37.0	37.0
5	36.15375	37.0	37.0	37.0	37.0	37.0
6	36.184	37.0	37.0	37.0	37.0	37.0
7	36.2795	37.0	37.0	37.0	37.0	37.0
8	36.1425	37.0	37.0	37.0	37.0	37.0
9	36.2505	37.0	37.0	37.0	37.0	37.0
10-14	36.25605	37.0	37.0	37.0	37.0	37.0
15-19	36.4054	37.0	37.0	37.0	37.0	37.0
20-24	36.37519999999999	37.0	37.0	37.0	37.0	37.0
25-29	36.2252	37.0	37.0	37.0	37.0	37.0
30-34	36.330600000000004	37.0	37.0	37.0	37.0	37.0
35-39	36.37705	37.0	37.0	37.0	37.0	37.0
40-44	36.28815	37.0	37.0	37.0	37.0	37.0
45-49	36.310649999999995	37.0	37.0	37.0	37.0	37.0
50-54	36.2495	37.0	37.0	37.0	37.0	37.0
55-59	36.25045	37.0	37.0	37.0	37.0	37.0
60-64	36.2975	37.0	37.0	37.0	37.0	37.0
65-69	36.2418	37.0	37.0	37.0	37.0	37.0
70-74	36.2685	37.0	37.0	37.0	37.0	37.0
75-79	36.243550000000006	37.0	37.0	37.0	37.0	37.0
80-84	36.1709	37.0	37.0	37.0	37.0	37.0
85-89	36.09955	37.0	37.0	37.0	37.0	37.0
90-94	36.185900000000004	37.0	37.0	37.0	37.0	37.0
95-99	36.132600000000004	37.0	37.0	37.0	37.0	37.0
100-104	36.10905863965992	37.0	37.0	37.0	37.0	37.0
105-109	36.01847230930144	37.0	37.0	37.0	37.0	37.0
110-114	36.05066776016788	37.0	37.0	37.0	37.0	37.0
115-119	35.936738952979205	37.0	37.0	37.0	37.0	37.0
120-124	35.90342588145912	37.0	37.0	37.0	37.0	37.0
125-129	35.800370374710006	37.0	37.0	37.0	37.0	37.0
130-134	35.708866058120016	37.0	37.0	37.0	37.0	37.0
135-139	35.75292290030417	37.0	37.0	37.0	37.0	37.0
140-144	35.66108542842608	37.0	37.0	37.0	37.0	37.0
145-149	35.69202046035805	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	5.0
20	3.0
21	4.0
22	2.0
23	6.0
24	5.0
25	4.0
26	8.0
27	17.0
28	9.0
29	18.0
30	12.0
31	37.0
32	62.0
33	104.0
34	192.0
35	359.0
36	2043.0
37	1106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.871871871871875	8.508508508508509	44.71971971971972	24.8998998998999
2	26.200000000000003	11.774999999999999	28.375	33.650000000000006
3	32.85	16.425	23.3	27.425
4	31.5	16.5	28.799999999999997	23.200000000000003
5	24.3	18.6	33.15	23.95
6	17.95	14.45	47.75	19.85
7	17.779444861215303	13.928482120530134	38.68467116779195	29.607401850462615
8	18.33416708354177	13.306653326663332	39.41970985492746	28.939469734867433
9	21.955488872218055	22.85571392848212	30.23255813953488	24.956239059764943
10-14	23.91119555977799	16.920846042302117	30.866543327166358	28.30141507075354
15-19	23.93	17.98	30.175	27.915
20-24	23.175	19.814999999999998	29.89	27.12
25-29	24.201210060503026	18.935946797339867	30.026501325066253	26.836341817090855
30-34	23.791189559477974	20.046002300115006	28.95644782239112	27.2063603180159
35-39	24.645	19.3	28.62	27.435
40-44	23.611180559027954	18.475923796189807	29.591479573978695	28.32141607080354
45-49	22.49	18.735	30.36	28.415000000000003
50-54	24.32	19.064999999999998	29.275000000000002	27.339999999999996
55-59	24.205	20.375	28.945	26.474999999999998
60-64	24.285	18.905	29.805	27.005000000000003
65-69	24.77	19.375	27.705000000000002	28.15
70-74	23.635	18.970000000000002	30.080000000000002	27.315
75-79	23.085	19.84	29.335	27.74
80-84	22.040000000000003	20.044999999999998	29.515	28.4
85-89	23.695	21.425	28.199999999999996	26.68
90-94	25.155	19.275000000000002	29.404999999999998	26.165
95-99	26.325	20.54	27.525	25.61
100-104	25.48254825482548	19.32193219321932	28.32783278327833	26.86768676867687
105-109	25.255408653846157	19.671474358974358	27.559094551282055	27.514022435897434
110-114	23.311929366910807	18.666599779271596	29.131132738035515	28.890338115782082
115-119	22.602670950898684	20.860528165478463	28.25082839642534	28.28597248719751
120-124	21.88428420363563	20.24271111334911	29.523138123772597	28.34986655924266
125-129	22.290708836457334	19.57257616329005	29.28813216793816	28.848582832314456
130-134	23.085499974659164	18.14403730170797	29.071005017485174	29.699457706147687
135-139	23.999593227233436	19.46407688005288	27.965627701225404	28.57070219148828
140-144	24.645444342414038	18.299153147637995	29.609223548617486	27.446178961330475
145-149	25.033248081841432	18.429667519181585	27.28388746803069	29.25319693094629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	0.5
26	1.0
27	2.0
28	1.0
29	3.0
30	4.5
31	4.5
32	7.0
33	7.0
34	12.0
35	13.0
36	15.5
37	39.5
38	47.0
39	32.0
40	31.5
41	56.5
42	93.0
43	100.5
44	97.5
45	158.0
46	180.0
47	138.0
48	149.5
49	217.0
50	252.5
51	243.0
52	258.0
53	234.0
54	227.5
55	236.0
56	182.5
57	177.0
58	216.0
59	175.0
60	114.5
61	82.0
62	40.5
63	19.0
64	6.5
65	2.0
66	5.5
67	7.0
68	9.0
69	13.5
70	12.0
71	5.0
72	4.5
73	22.5
74	27.5
75	10.5
76	2.0
77	1.0
78	1.5
79	1.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.025
8	0.05
9	0.025
10-14	0.005
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.005
35-39	0.0
40-44	0.005
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
102	1.0
103	0.0
104	2.0
105	2.0
106	1.0
107	3.0
108	0.0
109	2.0
110	1.0
111	1.0
112	2.0
113	0.0
114	0.0
115	0.0
116	1.0
117	1.0
118	2.0
119	6.0
120	0.0
121	5.0
122	0.0
123	1.0
124	3.0
125	0.0
126	9.0
127	5.0
128	0.0
129	1.0
130	3.0
131	2.0
132	1.0
133	4.0
134	2.0
135	3.0
136	2.0
137	4.0
138	2.0
139	2.0
140	2.0
141	4.0
142	1.0
143	6.0
144	3.0
145	0.0
146	0.0
147	0.0
148	0.0
149	3910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.27461139896373	28.599999999999998
2	20.932642487046632	20.200000000000003
3	7.875647668393783	11.4
4	3.7823834196891193	7.3
5	2.6424870466321244	6.375
6	1.450777202072539	4.2
7	0.8290155440414507	2.8000000000000003
8	0.6217616580310881	2.4
9	0.5699481865284974	2.475
>10	2.0207253886010363	14.249999999999998
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGGTTTCGGGGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTG	46	1.15	No Hit
TCCTTTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGGCTAATACTCTG	38	0.95	No Hit
TGGTTTTTCCCTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAAG	26	0.65	No Hit
TGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAAGGCCCCTCCGTC	24	0.6	No Hit
TCCTCCTTGCCCTTTCGTCATCTTCTCCACGGTGCTCACGTGGACCTCTG	18	0.44999999999999996	No Hit
TGGGTTCCTTCATGCTCAGGAGCAGCTGTTTCCTGCTCATGTTCTTCACT	17	0.42500000000000004	No Hit
TCACTTCTCTTTGAAGGTCAGAATGTAGGATGCTTTCAGGAGCAGGAGTT	17	0.42500000000000004	No Hit
TAGGTATTGCCGACATTGGTTCCAGGTGGAGAAAGCAGGACCTTAACTCT	16	0.4	No Hit
TCCCGACGCTTCCGCTGCGGGCCCATGGCCACTGAGCTCCAGCTCGCTCG	15	0.375	No Hit
TGGGACTAGGTATTGCCGACATTGGTTCCAGGTGGAGAAAGCAGGACCTT	15	0.375	No Hit
TCTGTTTCCTCCTTTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGGCT	14	0.35000000000000003	No Hit
CTGGCTCTAGCTCCCGATGCAGCCTCAGGATTGTCGGGGTGCTCTGCAGG	14	0.35000000000000003	No Hit
TCCCTTTGGCTCCTGTAGGTCGTCCTCTTCTGTCTGGGCCTTGTTCATTT	14	0.35000000000000003	No Hit
GGGCTTCTCAAAGCTTCGCTATGGCTGCACACCCATTATATACTGCATAA	14	0.35000000000000003	No Hit
TCCTCTACCTCCTCCACCTTCTCCAGTGCTTTCGGCCCTGCTCTCTGCAG	13	0.325	No Hit
CGGGGAGTCGGACAGCTGGGAAACTGCAGACACCATGTCTGTGGTCTCTT	13	0.325	No Hit
GCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGACACTCTG	13	0.325	No Hit
GGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGACACTC	12	0.3	No Hit
TGGGGTCTCAGAGCCAAGTCGTCTTTTCCTCTGGTGTTAAACTCTTACTT	12	0.3	No Hit
CTGTACCTTGATTCTCTCCTGAGAGGACAGCATCTCCACCTCACTGACAA	12	0.3	No Hit
ACAGTTTCTTCGACCTTTTTCACAGCTTCGGTTGTTTGAGTGACTGGTGT	12	0.3	No Hit
GACTAGGTATTGCCGACATTGGTTCCAGGTGGAGAAAGCAGGACCTTAAC	12	0.3	No Hit
CTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAAGGCCCCTCCGT	12	0.3	No Hit
GGAGGACTACATAAAACATACATATAAATACATAAAGGAGGAGGCTCCAG	12	0.3	No Hit
GGGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGACACT	12	0.3	No Hit
GTTCCTTCATGCTCAGGAGCAGCTGTTTCCTGCTCATGTTCTTCACTGGC	12	0.3	No Hit
TCCGGGGAGTCGGACAGCTGGGAAACTGCAGACACCATGTCTGTGGTCTC	11	0.27499999999999997	No Hit
CCTTGTTCATTTCTTCTTTCAGGGCATCTCCTTCCTCGGTTAGCTTTGGT	11	0.27499999999999997	No Hit
GAGACTTGAAGGTCGCTTTCATCTTTGTCCAGCATCTGTTGTGCTCCCTG	11	0.27499999999999997	No Hit
TCCAGTTCTTTAACACACTGTCCGTCTTTGACTTTTTCTCCATCAAACTG	11	0.27499999999999997	No Hit
TGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAAGGCCCCTCCGTCTG	11	0.27499999999999997	No Hit
TTCTCTTTCTCGGTAGCCAGCACCTCGGAGTCCTCCTCTACCTCCTCCAC	10	0.25	No Hit
TCGTCATGGGCATCTTTGCCCCCTGGTAAAGTCTGGGCGAGTGCAGGAGT	10	0.25	No Hit
TCGGTTAGCTTTGGTCCGTTGGTGTCTGGGGACTCTTTGGTTAGATTTCC	10	0.25	No Hit
GCCGTCAGCAGCATGTTCCTTTTCATCTTTGCACTGAAGAGGCAGCTTCT	10	0.25	No Hit
TTGGTGTCTGGGGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGC	10	0.25	No Hit
CTCACTTCTCTTTGAAGGTCAGAATGTAGGATGCTTTCAGGAGCAGGAGT	10	0.25	No Hit
GTAAAGTCTGGGCGAGTGCAGGAGTTGCTTCTGCAGTGATGTCTTTTTCA	10	0.25	No Hit
TTGGGGCTTCTCAAAGCTTCGCTATGGCTGCACACCCATTATATACTGCA	10	0.25	No Hit
TTCCTTCATGCTCAGGAGCAGCTGTTTCCTGCTCATGTTCTTCACTGGCT	9	0.22499999999999998	No Hit
TCCTCTCTTCCCCTTCTGCTTCTTCCCGGAGAGCTTCTTTAAGCCCGAAC	9	0.22499999999999998	No Hit
TGGGAGAGAAGGGCTTCCTTCAAGGCTGCCCAGTGCCTTTCCCCTCTCTG	9	0.22499999999999998	No Hit
TCAGGACTGGCTTAGGGTGGGTTCCTTCATGCTCAGGAGCAGCTGTTTCC	9	0.22499999999999998	No Hit
TCGGTTTTCTTCTCCATCTTTGGCTGCTTCTTCCTCGGTCCCTTGACCAG	9	0.22499999999999998	No Hit
TCCGGGCTAATACTCTGTTCTTCATTACTCTCAGTTTCCTGACATCTGGC	9	0.22499999999999998	No Hit
TGGTTTCTGCGGCTGTCACTGCTTCTGAGGTAAAATCCACTTCACTGGTG	9	0.22499999999999998	No Hit
TTGGGACATTCTGAATGTCTCAAAATGGAGTTTTTATTTCTGAGCTCACT	9	0.22499999999999998	No Hit
CACGTTCTCCTGAGCTCGCTGCGCTTCCAGCTTCTCTCTCTCTACCGCAT	9	0.22499999999999998	No Hit
CCGTATCTAGCATGCCACCCTCTACCTCCTGAACTGGGGTGGCTTCCTCT	9	0.22499999999999998	No Hit
TGGGGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGCTA	9	0.22499999999999998	No Hit
TCCTCTTCTGTCTGGGCCTTGTTCATTTCTTCTTTCAGGGCATCTCCTTC	8	0.2	No Hit
TCAAGGTTTCAGATGACTCAGGGGCCACAGCCTGTTCCATCAGCTGCTGG	8	0.2	No Hit
GGACATTCTGAATGTCTCAAAATGGAGTTTTTATTTCTGAGCTCACTGTG	8	0.2	No Hit
TACCTTGATTCTCTCCTGAGAGGACAGCATCTCCACCTCACTGACAATGC	8	0.2	No Hit
TCGGCGTGTCGCTCTCCGCCGGCTGCTCGGGGCTCCGCTGCTCGGTGGAA	8	0.2	No Hit
GGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGACACTCT	8	0.2	No Hit
CTCGGTTAGCTTTGGTCCGTTGGTGTCTGGGGACTCTTTGGTTAGATTTC	8	0.2	No Hit
CTCCTCCTTGCCCTTTCGTCATCTTCTCCACGGTGCTCACGTGGACCTCT	8	0.2	No Hit
CTCCCGACGCTTCCGCTGCGGGCCCATGGCCACTGAGCTCCAGCTCGCTC	8	0.2	No Hit
TTGAAGGTCGCTTTCATCTTTGTCCAGCATCTGTTGTGCTCCCTGGCTGT	8	0.2	No Hit
TTTTTCCCTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAAGGCC	8	0.2	No Hit
GGCCGTCAGCAGCATGTTCCTTTTCATCTTTGCACTGAAGAGGCAGCTTC	8	0.2	No Hit
CTGGTTTTTCCCTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAA	7	0.17500000000000002	No Hit
TTCCTGGTTTTTCCCTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTT	7	0.17500000000000002	No Hit
TCTGGGGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGC	7	0.17500000000000002	No Hit
TTGGCTAAGCTTTGGTGCTGGGGGCCGTCAGCAGCATGTTCCTTTTCATC	7	0.17500000000000002	No Hit
TTTCACTTTGTCTGCAACGGCTTGGAGGACGGCCTGCGTCTGGCGCTCCT	7	0.17500000000000002	No Hit
CAGTCTTGAAAAGAGGAAATGTTAGCTGGCTCTAGCTCCCGATGCAGCCT	7	0.17500000000000002	No Hit
TGGTGTGTCTGAGCTTTGAACTTGAACCTCTATGCAATCTGCTTTGTCTT	7	0.17500000000000002	No Hit
TCCTCTTCAGTGACCTGTGACTGCTTGACGGCGGCAATGGCATTACTGTC	7	0.17500000000000002	No Hit
GGACTAGGTATTGCCGACATTGGTTCCAGGTGGAGAAAGCAGGACCTTAA	7	0.17500000000000002	No Hit
TCGGTAGCCAGCACCTCGGAGTCCTCCTCTACCTCCTCCACCTTCTCCAG	7	0.17500000000000002	No Hit
GTTGGTGTCTGGGGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTG	7	0.17500000000000002	No Hit
GTGGGTTTCGGGGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCT	7	0.17500000000000002	No Hit
GGGAGACTTGAAGGTCGCTTTCATCTTTGTCCAGCATCTGTTGTGCTCCC	7	0.17500000000000002	No Hit
GGGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGCTAAG	7	0.17500000000000002	No Hit
GTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGACACTCTGGAC	7	0.17500000000000002	No Hit
TCTTCATCAGCTCCTCCACAGGCTCAGCTTCCTGGGGGACCTCCTGGGTC	7	0.17500000000000002	No Hit
GGGCTAATACTCTGTTCTTCATTACTCTCAGTTTCCTGACATCTGGCCAC	6	0.15	No Hit
GACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGCTAAGCT	6	0.15	No Hit
CTGGCTCTTCATCTCCCCCGCCTCCTCCTCTCTTCCCCTTCTGCTTCTTC	6	0.15	No Hit
TTGGTCTTGTTCCTGGGTGCTGGCAGGAAGAGCATCTGCTTCTTTGTCTT	6	0.15	No Hit
CTGCTCATGTTCTTCACTGGCTTGCTCTGTCTTGGTCTCTGTTTCCTCCT	6	0.15	No Hit
TTGTTTGAGTGACTGGTGTGTCTGAGCTTTGAACTTGAACCTCTATGCAA	6	0.15	No Hit
GGGGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGACAC	6	0.15	No Hit
TCTGGGGTAGCCTGTACAGTCTCGGAGCCCTGTGCAAGATGCTCAGTTTC	6	0.15	No Hit
GGAAACTGTAGACTTATCATAAATAGTGGCACACCATCTGGGCCTTGTCC	6	0.15	No Hit
TCTGAATCTGCGAGGGGAGTACTTCCATTTGTCTCACTGTCTGTCAAGGT	6	0.15	No Hit
TCTTTCACTTTGTCTGCAACGGCTTGGAGGACGGCCTGCGTCTGGCGCTC	6	0.15	No Hit
GGGACTAGGTATTGCCGACATTGGTTCCAGGTGGAGAAAGCAGGACCTTA	6	0.15	No Hit
CTGAACTGGGGTGGCTTCCTCTGTGGTGTCCGGGGAGTCGGACAGCTGGG	6	0.15	No Hit
GGCAGTCTTGAAAAGAGGAAATGTTAGCTGGCTCTAGCTCCCGATGCAGC	6	0.15	No Hit
CCTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTGTTGAAGGCCCCTCCG	6	0.15	No Hit
TGTCAACGGTCTCGTCTTGCTGTGTCCCGTTTGGAGTGTCTGAATCTGCG	6	0.15	No Hit
CTCTTTCACTTTGTCTGCAACGGCTTGGAGGACGGCCTGCGTCTGGCGCT	6	0.15	No Hit
GGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGCTAAGC	6	0.15	No Hit
TTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGGCTAATACTCTGTTCT	6	0.15	No Hit
GGGGAGTCGGACAGCTGGGAAACTGCAGACACCATGTCTGTGGTCTCTTC	6	0.15	No Hit
CCCCTTCCACCTCTGCTCCTCCTTGCCCTTTCGTCATCTTCTCCACGGTG	6	0.15	No Hit
GAGGACTACATAAAACATACATATAAATACATAAAGGAGGAGGCTCCAGT	6	0.15	No Hit
CTAATACTCTGTTCTTCATTACTCTCAGTTTCCTGACATCTGGCCACTTC	6	0.15	No Hit
CTTCTCAAAGCTTCGCTATGGCTGCACACCCATTATATACTGCATAATAC	6	0.15	No Hit
TCTCTCTTCCAGTTTTGACTTGGATTTTTTTCGTGGAGTGACTAATCTCT	6	0.15	No Hit
CCTTCCTGGTTTTTCCCTGTGTTCTTCCTGGGCTGGAAAACTAGATGGTG	6	0.15	No Hit
CCGTTTGGAGTGTCTGAATCTGCGAGGGGAGTACTTCCATTTGTCTCACT	6	0.15	No Hit
TCTGTCTTGGTCTCTGTTTCCTCCTTTTCAACGTCGGTTCCCATCTCTCT	6	0.15	No Hit
GGGCTCTTGGTGGTCTCCTGCTCCGACGGAGGCTTCTGCCCCTTCGCCTT	5	0.125	No Hit
TTATCTTCATCTGATCTCTGTTTCTGGGATGCGCTCACTTCTCTTTGAAG	5	0.125	No Hit
TGTGTCTGAGCTTTGAACTTGAACCTCTATGCAATCTGCTTTGTCTTGGT	5	0.125	No Hit
TTGATTCTCTCCTGAGAGGACAGCATCTCCACCTCACTGACAATGCCTTC	5	0.125	No Hit
TGGCTTGCTCTGTCTTGGTCTCTGTTTCCTCCTTTTCAACGTCGGTTCCC	5	0.125	No Hit
TTTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGGCTAATACTCTGTTC	5	0.125	No Hit
CCCGCCTCCTCCTCTCTTCCCCTTCTGCTTCTTCCCGGAGAGCTTCTTTA	5	0.125	No Hit
TAAGCTTTGGTGCTGGGGGCCGTCAGCAGCATGTTCCTTTTCATCTTTGC	5	0.125	No Hit
GCTCCTGTAGGTCGTCCTCTTCTGTCTGGGCCTTGTTCATTTCTTCTTTC	5	0.125	No Hit
TCGGGGGCTGTTTCTGTACGTGCAAACTGGTCGACGGCTGTCTGGATGAC	5	0.125	No Hit
CGGTGTTTCCTCCTGCTCGTCCTTTGTGATATCTTCAACAACTGTGGAAC	5	0.125	No Hit
GCTTGGTCTTGTTCCTGGGTGCTGGCAGGAAGAGCATCTGCTTCTTTGTC	5	0.125	No Hit
TCCTCCTCTCTTCCCCTTCTGCTTCTTCCCGGAGAGCTTCTTTAAGCCCG	5	0.125	No Hit
TGGCTTAGGGTGGGTTCCTTCATGCTCAGGAGCAGCTGTTTCCTGCTCAT	5	0.125	No Hit
CCGACATTGGTTCCAGGTGGAGAAAGCAGGACCTTAACTCTGAAATACAT	5	0.125	No Hit
GGGGACTCTTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGCTAA	5	0.125	No Hit
CTCCTCGCTGTTGGCATCAAGAAGATCATCAATGTCAATTTCTACATCTG	5	0.125	No Hit
TGGCTGTCAACGGTCTCGTCTTGCTGTGTCCCGTTTGGAGTGTCTGAATC	5	0.125	No Hit
TTCCCTTTTCTTCTCTCCGTCGGAAGTCGCTCCTTCCTCAGCTTCCGCTT	5	0.125	No Hit
TCCTGAACTGGGGTGGCTTCCTCTGTGGTGTCCGGGGAGTCGGACAGCTG	5	0.125	No Hit
CAGCTGCTCCCGACGCTTCCGCTGCGGGCCCATGGCCACTGAGCTCCAGC	5	0.125	No Hit
CCTCTTTCACTTTGTCTGCAACGGCTTGGAGGACGGCCTGCGTCTGGCGC	5	0.125	No Hit
CCGACGCTTCCGCTGCGGGCCCATGGCCACTGAGCTCCAGCTCGCTCGGC	5	0.125	No Hit
CCTGTTCTTGCTTCCCATCTGCCCTTTTCTTCCGCCGTCCAGGAATAAAT	5	0.125	No Hit
TGTTCTTCCGTATCTAGCATGCCACCCTCTACCTCCTGAACTGGGGTGGC	5	0.125	No Hit
TCACCCTCTTGACCCACCTCAGCCTTTGCCAGAATAGGCACTGCCCCGGC	5	0.125	No Hit
GCTTCTCAAAGCTTCGCTATGGCTGCACACCCATTATATACTGCATAATA	5	0.125	No Hit
TCACGTGAAAAATTTCCACATCTGACCTCAAGTAATGTGTCATAGTCCTA	5	0.125	No Hit
TTTGGTTAGATTTCCAGAGGCATCCGCCTCTGCCTTGGCTAAGCTTTGGT	5	0.125	No Hit
CTGGCTGTCAACGGTCTCGTCTTGCTGTGTCCCGTTTGGAGTGTCTGAAT	5	0.125	No Hit
TCTCTGTTTCCTCCTTTTCAACGTCGGTTCCCATCTCTCTTTTCTCCGGG	5	0.125	No Hit
TCGCTTTCATCTTTGTCCAGCATCTGTTGTGCTCCCTGGCTGTCAGCCTG	5	0.125	No Hit
TCCCCATAAAATAAACTTTCTAAAGTATAAGCAGCAGAAATGGTGTAAAC	5	0.125	No Hit
TGGGATGCGCTCACTTCTCTTTGAAGGTCAGAATGTAGGATGCTTTCAGG	5	0.125	No Hit
CCAAAGCCAGCTTCAGTCACCACAAACCCTTCTTCGCCGACCAATTTCAG	5	0.125	No Hit
CAGTTTCTTCGACCTTTTTCACAGCTTCGGTTGTTTGAGTGACTGGTGTG	5	0.125	No Hit
GGTAAGTTCTTCAGCGCGGAGCGCCTCTGTGGTTTCTGCGGCTGTCACTG	5	0.125	No Hit
TCCTCTCCATGCTGAAGAGTCCCGTAGCCACCGGTTTCGGAGAGCTTCTC	5	0.125	No Hit
TTCTGTTGAACACCACTTGGCTGGAAACAGAGTTAATCTTGTATGAGGAA	5	0.125	No Hit
CGGGCTAATACTCTGTTCTTCATTACTCTCAGTTTCCTGACATCTGGCCA	5	0.125	No Hit
TTCGATCTCGGAGGCCAACTGCTCTGCACCGGAGTCTTCGGCTCTCTCTT	5	0.125	No Hit
TTTCCTCTTGGACGTGGACATCTTCTTGTTCAGCTACACCATTGACGGCA	5	0.125	No Hit
GGGGCCGTCAGCAGCATGTTCCTTTTCATCTTTGCACTGAAGAGGCAGCT	5	0.125	No Hit
TTGGGGGGAGACTTGAAGGTCGCTTTCATCTTTGTCCAGCATCTGTTGTG	5	0.125	No Hit
TGTGTCCCGTTTGGAGTGTCTGAATCTGCGAGGGGAGTACTTCCATTTGT	5	0.125	No Hit
TAATGATTGCGCACCTGCACACTCCAAACTTTCATCCATCTCTGAAGTTG	5	0.125	No Hit
TCTCTACTTGGTTCGATCTCGGAGGCCAACTGCTCTGCACCGGAGTCTTC	5	0.125	No Hit
TCAGGATTGTCGGGGTGCTCTGCAGGGTTGAAATCATTGGACGGCTCTGC	5	0.125	No Hit
TCACTGTCTGTCAAGGTTTCAGATGACTCAGGGGCCACAGCCTGTTCCAT	5	0.125	No Hit
GACTACATAAAACATACATATAAATACATAAAGGAGGAGGCTCCAGTTTG	5	0.125	No Hit
TCGTCTTGCTGTGTCCCGTTTGGAGTGTCTGAATCTGCGAGGGGAGTACT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR33623191 read2 length is 102-149 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR33623191_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	102-149
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0445	37.0	37.0	37.0	37.0	37.0
2	36.09125	37.0	37.0	37.0	37.0	37.0
3	36.2435	37.0	37.0	37.0	37.0	37.0
4	36.08475	37.0	37.0	37.0	37.0	37.0
5	36.22125	37.0	37.0	37.0	37.0	37.0
6	36.225	37.0	37.0	37.0	37.0	37.0
7	36.21875	37.0	37.0	37.0	37.0	37.0
8	36.178	37.0	37.0	37.0	37.0	37.0
9	36.17575	37.0	37.0	37.0	37.0	37.0
10-14	36.32995	37.0	37.0	37.0	37.0	37.0
15-19	36.32045	37.0	37.0	37.0	37.0	37.0
20-24	36.31555	37.0	37.0	37.0	37.0	37.0
25-29	36.156549999999996	37.0	37.0	37.0	37.0	37.0
30-34	36.227799999999995	37.0	37.0	37.0	37.0	37.0
35-39	36.244749999999996	37.0	37.0	37.0	37.0	37.0
40-44	36.2051	37.0	37.0	37.0	37.0	37.0
45-49	36.2328	37.0	37.0	37.0	37.0	37.0
50-54	36.256150000000005	37.0	37.0	37.0	37.0	37.0
55-59	36.2573	37.0	37.0	37.0	37.0	37.0
60-64	36.2285	37.0	37.0	37.0	37.0	37.0
65-69	36.16665	37.0	37.0	37.0	37.0	37.0
70-74	36.2074	37.0	37.0	37.0	37.0	37.0
75-79	36.21145	37.0	37.0	37.0	37.0	37.0
80-84	36.1615	37.0	37.0	37.0	37.0	37.0
85-89	36.08475	37.0	37.0	37.0	37.0	37.0
90-94	36.0606	37.0	37.0	37.0	37.0	37.0
95-99	36.01645	37.0	37.0	37.0	37.0	37.0
100-104	35.96675055013753	37.0	37.0	37.0	37.0	37.0
105-109	35.95318312664547	37.0	37.0	37.0	37.0	37.0
110-114	35.903361262891124	37.0	37.0	37.0	37.0	37.0
115-119	35.901790626761894	37.0	37.0	37.0	37.0	37.0
120-124	35.88994135005875	37.0	37.0	37.0	37.0	37.0
125-129	35.83881170740166	37.0	37.0	37.0	37.0	37.0
130-134	35.752063544758116	37.0	37.0	37.0	37.0	37.0
135-139	35.69176278151224	37.0	37.0	37.0	37.0	37.0
140-144	35.6420430935362	37.0	37.0	37.0	37.0	37.0
145-149	35.56349206349206	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	2.0
20	0.0
21	1.0
22	5.0
23	5.0
24	7.0
25	6.0
26	5.0
27	14.0
28	12.0
29	10.0
30	22.0
31	34.0
32	71.0
33	110.0
34	218.0
35	448.0
36	2262.0
37	765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.012006003001503	29.664832416208103	28.489244622311155	17.83391695847924
2	25.85	30.85	21.45	21.85
3	28.199999999999996	34.625	16.950000000000003	20.225
4	29.225	36.65	16.45	17.675
5	24.925	37.6	18.75	18.725
6	25.674999999999997	23.45	27.700000000000003	23.175
7	26.35	23.9	22.15	27.6
8	27.125	21.975	23.625	27.275
9	24.375	36.15	18.35	21.125
10-14	27.91139556977849	26.281314065703288	20.126006300315016	25.681284064203208
15-19	28.7914395719786	28.311415570778536	19.29096454822741	23.60618030901545
20-24	28.305000000000003	28.235	19.685	23.775
25-29	28.610000000000003	28.249999999999996	19.75	23.39
30-34	27.939999999999998	28.305000000000003	19.23	24.525
35-39	27.855	27.785	19.575	24.785
40-44	27.339999999999996	27.395000000000003	20.18	25.085
45-49	28.294999999999998	28.265	19.085	24.355
50-54	28.634999999999998	28.754999999999995	19.12	23.49
55-59	28.765	27.644999999999996	19.05	24.54
60-64	28.294999999999998	28.42	18.925	24.36
65-69	28.376418820941048	27.256362818140907	19.790989549477477	24.576228811440572
70-74	27.889999999999997	28.02	19.055	25.035
75-79	28.355000000000004	27.994999999999997	19.405	24.245
80-84	28.53	27.655	19.645000000000003	24.169999999999998
85-89	27.655	28.395	19.735	24.215
90-94	28.845	27.639999999999997	19.52	23.995
95-99	27.529999999999998	28.970000000000002	19.49	24.01
100-104	27.9027902790279	28.967896789678964	19.656965696569657	23.472347234723472
105-109	28.165064102564102	28.80608974358974	19.345953525641026	23.682892628205128
110-114	27.6863650045149	29.085983746363	18.817096418179993	24.410554830942107
115-119	28.090169695752586	29.109348328145394	19.4698262877799	23.33065568832212
120-124	28.098091545395036	29.100156100508585	19.129865552142604	23.67188680195377
125-129	27.545091699085532	29.070883645733343	18.668216035972314	24.71580861920881
130-134	28.559120166235875	29.101414018549495	18.691399320865642	23.648066494348992
135-139	28.0301103707848	28.503128019937947	19.220792431717616	24.245969177559637
140-144	28.094533204022255	28.895921596651526	19.406870501761013	23.60267469756521
145-149	28.607270865335384	29.242191500256016	18.960573476702507	23.189964157706093
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	1.0
28	4.0
29	3.5
30	3.0
31	5.0
32	5.5
33	6.0
34	12.5
35	17.5
36	15.0
37	20.0
38	25.5
39	39.0
40	45.5
41	44.0
42	64.0
43	67.5
44	87.5
45	123.0
46	159.0
47	185.5
48	160.0
49	171.5
50	229.0
51	241.0
52	239.0
53	233.0
54	227.5
55	206.5
56	178.0
57	208.0
58	218.0
59	184.0
60	149.5
61	99.5
62	52.0
63	33.0
64	20.5
65	43.0
66	45.5
67	14.0
68	23.0
69	22.0
70	8.5
71	7.0
72	7.0
73	15.0
74	15.5
75	6.5
76	4.0
77	3.0
78	1.0
79	1.0
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.005
15-19	0.005
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.005
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.005104124132298897
145-149	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
102	1.0
103	0.0
104	2.0
105	2.0
106	1.0
107	3.0
108	0.0
109	2.0
110	1.0
111	1.0
112	2.0
113	0.0
114	0.0
115	0.0
116	1.0
117	1.0
118	2.0
119	6.0
120	0.0
121	5.0
122	0.0
123	1.0
124	3.0
125	0.0
126	9.0
127	5.0
128	0.0
129	1.0
130	3.0
131	2.0
132	1.0
133	4.0
134	3.0
135	3.0
136	2.0
137	4.0
138	3.0
139	2.0
140	2.0
141	4.0
142	1.0
143	6.0
144	5.0
145	0.0
146	0.0
147	0.0
148	0.0
149	3906.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.54529307282416	34.65
2	20.515097690941385	23.1
3	9.236234458259325	15.6
4	4.129662522202486	9.3
5	1.7317939609236235	4.875
6	1.0213143872113677	3.45
7	0.8436944937833037	3.325
8	0.2664298401420959	1.2
9	0.3108348134991119	1.575
>10	0.3996447602131439	2.9250000000000003
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCTCAGCAGGATCGTGGAGAGCGGGTGCTCCTCACCCCTTAGTCCCTGT	19	0.475	No Hit
TGTGTTCTCAGCAGGATCGTGGAGAGCGGGTGCTCCTCACCCCTTAGTCC	16	0.4	No Hit
TTCTAGAACCCACACAAGCGCTGGCTGCCGGGGCAGTGCCTATTCTGGCA	16	0.4	No Hit
TCAGCAGGATCGTGGAGAGCGGGTGCTCCTCACCCCTTAGTCCCTGTGGT	14	0.35000000000000003	No Hit
TGGATTTTACCTCAGAAGCAGTGACAGCCGCAGAAACCACAGAGGCGCTC	11	0.27499999999999997	No Hit
GAACACTCAGTGCCGTAGCCCAGGAAGGACTTGCGGTTTCTGATAGTTCT	11	0.27499999999999997	No Hit
AAAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCAC	10	0.25	No Hit
GACAATCCTGAGGCTGCATCGGGAGCTAGAGCCAGCTAACATTTCCTCTT	10	0.25	No Hit
TGAAAGCGACCTTCAAGTCTCCCCCCAAGATGGAACACTCAGTGCCGTAG	10	0.25	No Hit
GAGAAGTGAGCGCATCCCAGAAACAGAGATCAGATGAAGATAACAAGCCA	9	0.22499999999999998	No Hit
GCAAAGATGCCCATGACGACATAGTCACCAGTGAAGTGGATTTTACCTCA	9	0.22499999999999998	No Hit
TGAAACTGAGCATCTTGCACAGGGCTCCGAGACTGTACAGGCTACCCCAG	9	0.22499999999999998	No Hit
ACGGAACAAGATGCTGCTGTTGGGACCTTGAGACCAAAATTTCAGAGCCC	9	0.22499999999999998	No Hit
CGAAACCCACGCTTCTGATTTACAGAATCAGGTTCCTGTGATGCAGGCTG	9	0.22499999999999998	No Hit
AAGGATGTTGTGCCGGAAGGACCCGTGCAGGAAGCTGAAACTGAGCATCT	9	0.22499999999999998	No Hit
CAAGGCTTCAGAAATGATCACCACGCTTGCAGTTGAAAGTGCCAGTGTCA	9	0.22499999999999998	No Hit
TTAAAGAACTGGAGGTGCCTGTGCACACTGGACCCAACAGTCAAAAGACT	8	0.2	No Hit
TCTGGTTCACACCGTGAGTGTCGCGGTCATTGATGGGACCAGGGCAGTCA	8	0.2	No Hit
CCAGTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGG	8	0.2	No Hit
TCTCAGGCAGAGTCCATCCCAATAATAGTAACTCCTGCTCCTGAAAGCAT	8	0.2	No Hit
GGGAACCGACGTTGAAAAGGAGGAAACAGAGACCAAGACAGAGCAAGCCA	8	0.2	No Hit
AAAGAACTGGAGGTGCCTGTGCACACTGGACCCAACAGTCAAAAGACTGC	8	0.2	No Hit
AGAGGGTGAGGCTGGCCAGTTTGATGGAGAAAAAGTCAAAGACGGACAGT	7	0.17500000000000002	No Hit
ACAGACATGGTGTCTGCAGTTTCCCAGCTGTCCGACTCCCCGGACACCAC	7	0.17500000000000002	No Hit
AGGACTCCGAGGTGCTGGCTACCGAGAAAGAGAAGGATGTTGTGCCGGAA	7	0.17500000000000002	No Hit
TTCTGAAGGCATGAGCAAGGCTTCAGAAATGATCACCACGCTTGCAGTTG	7	0.17500000000000002	No Hit
CCTATTCTGGCAAAGGCTGAGGTGGGTCAAGAGGGTGAGGCTGGCCAGTT	7	0.17500000000000002	No Hit
TGCCGTAGCCCAGGAAGGACTTGCGGTTTCTGATAGTTCTGAAGGCATGA	7	0.17500000000000002	No Hit
GGAGACCTGACAGAATCCTAAGACGTGAGTTGCTCATTGTAAAACTAGAA	7	0.17500000000000002	No Hit
ACCGTGCCACCACATGCCAGGTTATCAAGCACCAGCAGCTGATGGAACAG	7	0.17500000000000002	No Hit
GCTGGCCAGTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTAAAGA	7	0.17500000000000002	No Hit
TGAAACCTTGACAGACAGTGAGACAAATGGAAGTACTCCCCTCGCAGATT	7	0.17500000000000002	No Hit
GTGAGTGTCGCGGTCATTGATGGGACCAGGGCAGTCACCAGTGCCGAAGA	7	0.17500000000000002	No Hit
CTGAAGTCACAGAGGATGTAGACCGTGCCACCACATGCCAGGTTATCAAG	7	0.17500000000000002	No Hit
AAGACGGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCACACTGGACCC	7	0.17500000000000002	No Hit
ACACAGGGAAAAACCAGGAAGGGATGTTCTAGAACCCACACAAGCGCTGG	7	0.17500000000000002	No Hit
GAGAGCGGGTGCTCCTCACCCCTTAGTCCCTGTGGTCCTGGGCGCACATC	7	0.17500000000000002	No Hit
GAAAAACCAGGAAGGGATGTTCTAGAACCCACACAAGCGCTGGCTGCCGG	7	0.17500000000000002	No Hit
GCTAACCGAGGAAGGAGATGCCCTGAAAGAAGAAATGAACAAGGCCCAGA	7	0.17500000000000002	No Hit
AAGAGACCACAGACATGGTGTCTGCAGTTTCCCAGCTGTCCGACTCCCCG	7	0.17500000000000002	No Hit
TGAAAAAGACATCACTGCAGAAGCAACTCCTGCACTCGCCCAGACTTTAC	7	0.17500000000000002	No Hit
AGAATCCTTGCCCCCTGAGAAACTGGCTGAGACCCAGGAGGTCCCCCAGG	6	0.15	No Hit
TACATTCTGACCTTCAAAGAGAAGTGAGCGCATCCCAGAAACAGAGATCA	6	0.15	No Hit
CCGGAAGGACCCGTGCAGGAAGCTGAAACTGAGCATCTTGCACAGGGCTC	6	0.15	No Hit
GACTTGCGGTTTCTGATAGTTCTGAAGGCATGAGCAAGGCTTCAGAAATG	6	0.15	No Hit
GTGAAGTCACGGAACAAGATGCTGCTGTTGGGACCTTGAGACCAAAATTT	6	0.15	No Hit
GGAAGTGTTTGATGAGAAGACGGAAGCCCACCAAGAAGTTGTTGCAGAGG	6	0.15	No Hit
AGAAGTGAGCGCATCCCAGAAACAGAGATCAGATGAAGATAACAAGCCAG	6	0.15	No Hit
GACGACCTACAGGAGCCAAAGGGAGACCTGACAGAATCCTAAGACGTGAG	6	0.15	No Hit
GAGAGTGCAGCAAGAGAGAAAATCCTCAGGGCTGAACCTGAGATCTTGGA	6	0.15	No Hit
GGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCACGTGAGCACCGTGGAGA	6	0.15	No Hit
TTGAGACCAAAATTTCAGAGCCCTTGAGGTGCAGAGAGCAGAGCCGTCCA	6	0.15	No Hit
GAGATGCCCTGAAAGAAGAAATGAACAAGGCCCAGACAGAAGAGGACGAC	6	0.15	No Hit
TGACAGAATCCTAAGACGTGAGTTGCTCATTGTAAAACTAGAATGTGAAG	6	0.15	No Hit
TCACCAGTGCCGAAGAGCGGTCCCCTTCGTGGATATCTGCTTCCATGACA	6	0.15	No Hit
AACCACAGAGGCGCTCCGCGCTGAAGAACTTACCGAAGCATCAGGGGCAG	6	0.15	No Hit
TCAGAAGCAGTGACAGCCGCAGAAACCACAGAGGCGCTCCGCGCTGAAGA	6	0.15	No Hit
CAGGAGCCTGCAGAAGGCACAGACCAGGCCAGGTTGTCAGCCGACTATGA	6	0.15	No Hit
AAGTGAGCGCATCCCAGAAACAGAGATCAGATGAAGATAACAAGCCAGAT	6	0.15	No Hit
TCAAACAACCGAAGCTGTGAAAAAGGTCGAAGAAACTGTGGCAACTTCAG	6	0.15	No Hit
AGGACTCCCAGGTGCCTGCAACCCAGACTCTGCAGAGAGCAGGGCCGAAA	6	0.15	No Hit
TAGAACCCACACAAGCGCTGGCTGCCGGGGCAGTGCCTATTCTGGCAAAG	6	0.15	No Hit
GTGGAGAGCGGGTGCTCCTCACCCCTTAGTCCCTGTGGTCCTGGGCGCAC	6	0.15	No Hit
GATATCTGCTTCCATGACAGAACCTCTTGAGCACGCAGAGGGAGTGGCCA	6	0.15	No Hit
TCAGAGTTAAGGTCCTGCTTTCTCCACCTGGAACCAATGTCGGCAATACC	5	0.125	No Hit
TACAGGAGCCAAAGGGAGACCTGACAGAATCCTAAGACGTGAGTTGCTCA	5	0.125	No Hit
ATCAGATGAAGATAACAAGCCAGATGCTGGTCCTGATGCTGCCGGCAAGG	5	0.125	No Hit
ACGTGAGTTGCTCATTGTAAAACTAGAATGTGAAGTGAAGTCACGGAACA	5	0.125	No Hit
CACAGACATGGTGTCTGCAGTTTCCCAGCTGTCCGACTCCCCGGACACCA	5	0.125	No Hit
GAAAAGACAGAGCCAGCCCCAGCCGAGGAGCAGGAGCCTGCAGAAGGCAC	5	0.125	No Hit
CCGAAGAGCGGTCCCCTTCGTGGATATCTGCTTCCATGACAGAACCTCTT	5	0.125	No Hit
CAGGATCCAGCAGCAGCTCCGGCTCCTCAAGGGAGGACTCGGCGCCGGTG	5	0.125	No Hit
GAGGAGGTAGAGGAGGACTCCGAGGTGCTGGCTACCGAGAAAGAGAAGGA	5	0.125	No Hit
AAAGATGAAAGCGACCTTCAAGTCTCCCCCCAAGATGGAACACTCAGTGC	5	0.125	No Hit
ACAGAGACCAAGACAGAGCAAGCCAGTGAAGAACATGAGCAGGAAACAGC	5	0.125	No Hit
TCCAGACAGCCGTCGACCAGTTTGCACGTACAGAAACAGCCCCCGAAACC	5	0.125	No Hit
CACATGCCAGGTTATCAAGCACCAGCAGCTGATGGAACAGGCTGTGGCCC	5	0.125	No Hit
GGTGAGGCTGGCCAGTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGT	5	0.125	No Hit
CAAGACAAAGCAGATTGCATAGAGGTTCAAGTTCAAAGCTCAGACACACC	5	0.125	No Hit
AAGAACTGGAGGTGCCTGTGCACACTGGACCCAACAGTCAAAAGACTGCT	5	0.125	No Hit
TCAGGCAGAGTCCATCCCAATAATAGTAACTCCTGCTCCTGAAAGCATCC	5	0.125	No Hit
CTAGAACCCACACAAGCGCTGGCTGCCGGGGCAGTGCCTATTCTGGCAAA	5	0.125	No Hit
GACACCGTGCCCCAGGGGCCTGAGTCTCAGGCAGAGTCCATCCCAATAAT	5	0.125	No Hit
GGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCACACTGGACCCAACAG	5	0.125	No Hit
GTGAAGTGAAGTCACGGAACAAGATGCTGCTGTTGGGACCTTGAGACCAA	5	0.125	No Hit
ACAGAGGCGCTCCGCGCTGAAGAACTTACCGAAGCATCAGGGGCAGAAGA	5	0.125	No Hit
TTTTACCTCAGAAGCAGTGACAGCCGCAGAAACCACAGAGGCGCTCCGCG	5	0.125	No Hit
AAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCACA	5	0.125	No Hit
GATAGTTCTGAAGGCATGAGCAAGGCTTCAGAAATGATCACCACGCTTGC	5	0.125	No Hit
AAAATTTCAGAGCCCTTGAGGTGCAGAGAGCAGAGCCGTCCAATGATTTC	5	0.125	No Hit
TTTCAACCCTGCAGAGCACCCCGACAATCCTGAGGCTGCATCGGGAGCTA	5	0.125	No Hit
TTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGGAGGT	5	0.125	No Hit
TCTCCGAAACCGGTGGCTACGGGACTCTTCAGCATGGAGAGGACACCGTG	5	0.125	No Hit
GATGTTCTAGAACCCACACAAGCGCTGGCTGCCGGGGCAGTGCCTATTCT	5	0.125	No Hit
AAGCCAGATGCTGGTCCTGATGCTGCCGGCAAGGAGAGTGCAGCAAGAGA	5	0.125	No Hit
CGCACTCTGCAGCAGCATGAGCGTCCGCCTCCCTCTCCTGCTTCGCCAGC	5	0.125	No Hit
AAAAAGACATCACTGCAGAAGCAACTCCTGCACTCGCCCAGACTTTACCA	5	0.125	No Hit
CCAGATGTCAGGAAACTGAGAGTAATGAAGAACAGAGTATTAGCCCGGAG	5	0.125	No Hit
GAGGAAGAAGGGGAAAAGACAGAGCCAGCCCCAGCCGAGGAGCAGGAGCC	5	0.125	No Hit
AAGAGGACGACCTACAGGAGCCAAAGGGAGACCTGACAGAATCCTAAGAC	5	0.125	No Hit
TGAGGCTGGCCAGTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTA	5	0.125	No Hit
AGGAAGGGATGTTCTAGAACCCACACAAGCGCTGGCTGCCGGGGCAGTGC	5	0.125	No Hit
GCCAGATGTCAGGAAACTGAGAGTAATGAAGAACAGAGTATTAGCCCGGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCTTG	10	0.0073195035	141.6875	8
TGGCCTT	10	0.0073195035	141.6875	7
>>END_MODULE
Rejected 433897 READS because READLEN < 1
Read 3145315 spots for SRR33623191.sra
Written 3145315 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
Read 3145311 spots for SRR33623191.sra
Written 3145311 spots for SRR33623191.sra
SRR ids: ['SRR33623191.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ftw8r30d
SRR33623191.sra spots: 25162492
blocks: [[1, 3145311], [3145312, 6290622], [6290623, 9435933], [9435934, 12581244], [12581245, 15726555], [15726556, 18871866], [18871867, 22017177], [22017178, 25162492]]
SRR33623191 file size 8349600
SRR33623191 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR33623191 SRR33623191_1.fastq SRR33623191_2.fastq
Input file:	SRR33623191_1.fastq
Paired file:	SRR33623191_2.fastq
trimmed:	SRR33623191-trimmed-pair1.fastq, SRR33623191-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Sun Mar  1 08:40:53 2026 >> started

Sun Mar  1 08:44:40 2026 >> done (227.729s)
24881013 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
24881013 (100.00%) read pairs available; of these:
   11276 ( 0.05%) trimmed read pairs available after processing
24869737 (99.95%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 98	       3	  0.00%
 99	       0	  0.00%
100	    6090	  0.02%
101	    6157	  0.02%
102	    6630	  0.03%
103	    6950	  0.03%
104	    6951	  0.03%
105	    7410	  0.03%
106	    8008	  0.03%
107	    8037	  0.03%
108	    8238	  0.03%
109	    8492	  0.03%
110	    9218	  0.04%
111	   10075	  0.04%
112	   10660	  0.04%
113	   11331	  0.05%
114	   10710	  0.04%
115	   11456	  0.05%
116	   11363	  0.05%
117	   11996	  0.05%
118	   12496	  0.05%
119	   13168	  0.05%
120	   13441	  0.05%
121	   14498	  0.06%
122	   14730	  0.06%
123	   15881	  0.06%
124	   17470	  0.07%
125	   16506	  0.07%
126	   17797	  0.07%
127	   17918	  0.07%
128	   17971	  0.07%
129	   18843	  0.08%
130	   20673	  0.08%
131	   21023	  0.08%
132	   21887	  0.09%
133	   22865	  0.09%
134	   23283	  0.09%
135	   24457	  0.10%
136	   25486	  0.10%
137	   27625	  0.11%
138	   27354	  0.11%
139	   27557	  0.11%
140	   28607	  0.11%
141	   27618	  0.11%
142	   30182	  0.12%
143	   31530	  0.13%
144	   34297	  0.14%
145	    5295	  0.02%
146	   18178	  0.07%
147	      44	  0.00%
148	   10993	  0.04%
149	24101565	 96.87%
24881013 reads passed initial QC


criterion=sequence-density
sequence-density=0.49
sequence-density-rank=1
fanout-score=2.62
fanout-score-rank=37
prefix-density=0.56
prefix-fanout=2.3
sequence=ATGATGGGCAGG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=44
fanout-score=71.36
fanout-score-rank=1
prefix-density=0.15
prefix-fanout=2.6
sequence=TTTGAGGGGTATATACTTTATTGAAAACTTTAAATACTCTTCAGAGAGAACATCACATCTTCAATCAAGGCTCTTGTGTGGCCTGCATGGAAGGAAACCTGAGCTCTCGCTTGATATGGAGAGATCAGTTGCAGTGCTGCCTTTGGCTGGAGTCCCTCACGACCCGTGCTCTTCTGCCAGCTTATCAGCCTTTGCCACGGCTTCTTCAATGGGTCCCACCATGTAGAAGGCTTGTTCTGGGAGATGGTCATATTCACCTGCTAAAATCTGCTGGAATCCTTTAATGGTCTCCTTCAAGGGCACCAGCTTCCCCATGTGACCCGTGAAGACCTCAGCAACTTGGAATGGCTGAGACAAGAAGCGCTGTATCTTTCTTGCCCGGGACACAGTCAATTTATCTTCCTCAGAAAGTTCATCCATACCCAAGATGGCAATGATGTCCTGGAGAGATTTGTAGTCCTGCAGGATTTTCTGCACTCCTCGGGCGACGTCATAATGCTCATTGCCAACA


criterion=sequence-density
sequence-density=1.73
sequence-density-rank=1
fanout-score=2.46
fanout-score-rank=36
prefix-density=1.85
prefix-fanout=2.3
sequence=GCACAGCCCCGAGGCCGGATTCTGGGTGGCCAGGAGGCCGCAGCCCATGCTCGGCCCTACATGGCTTCCGTGCAAGTGAACGGCACACACGTGTGCGGTGGCACCCTGCTGGACGAGCAGTGGGTGCTCAGTGCTGCACACTGCATGGATGGAGTGACGGATGACGACTCTGTGCAGGTGCTCCTGGGTGCCCACTCCCTGTCCGCCCCTGAACCCTACAAGCGATGGTATGATGTGCAGAGTGTAGTGCCTCACCCGGGCAGCCGACCTGACAGCCTTGAGGACGACCTCATTCTTTTTAAGCTATCCCAGAATGCCTCGTTGGGTCCCCACGTGAGACCCCTACCCTTGCAATACGAGGACAAAGAAGTGGAACCCGGCACGCTCTGCGACGTGGCTGGTTGGGGTGTGGTCACCCATGCAGGACGCAGGCCTGATGTCCTGCATCAACTCAGAGTGTCAATCATGAACCGGACAACCTGCAATCTGCGCACGTACCATGACGGGGTAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=141.10
fanout-score-rank=1
prefix-density=0.13
prefix-fanout=6.4
sequence=GCCGCCGCCATCGTCGGCGCGCTTCCCTGTTCACCTCTGACTCTGAGAATCCGTCGCCATCCGCCACCATGGTGAACTTCACAGTAGATCAGATCCGTGCCATCATGGACAAGAAAGCCAACATCCGGAACATGTCAGTCATCGCCCATGTGGACCACGGCAAGTCCACGCTGACCGACTCCCTTGTGTGCAAGGCTGGCATCATTGCCTCTGCCCGAGCTGGGGAGACGCGCTTCACTGACACTCGCAAGGATGAGCAGGAGCGCTGCATCACAATCAAATCCACCGCCATCTCCCTCTTCTACGAGCTCTCTGAGAACGACCTGAACTTCATTAAGCAGAGCAAGGATGGCTCG
SRR33623191 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Mar 01 08:45:02
                             Started mapping on |	Mar 01 08:45:02
                                    Finished on |	Mar 01 08:46:32
       Mapping speed, Million of reads per hour |	995.24

                          Number of input reads |	24881013
                      Average input read length |	296
                                    UNIQUE READS:
                   Uniquely mapped reads number |	20098503
                        Uniquely mapped reads % |	80.78%
                          Average mapped length |	297.53
                       Number of splices: Total |	17822818
            Number of splices: Annotated (sjdb) |	17638663
                       Number of splices: GT/AG |	17626430
                       Number of splices: GC/AG |	155626
                       Number of splices: AT/AC |	13981
               Number of splices: Non-canonical |	26781
                      Mismatch rate per base, % |	0.14%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.75
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.13
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3744359
             % of reads mapped to multiple loci |	15.05%
        Number of reads mapped to too many loci |	21793
             % of reads mapped to too many loci |	0.09%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.05%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1038151	1038151	1038151
N_multimapping	3744359	3744359	3744359
N_noFeature	1371599	19043816	1530493
N_ambiguous	1205954	104405	329898
UnstrandedReadsAssigned:17520950 PositiveStrandReadsAssigned:950282 NegativeStrandReadsAssigned:18238112
Dataset is classified negative stranded
MeadianReadLen=149 20thPercentileLength=149 echo kmer=145
SRR33623191 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 109,282
[index] number of k-mers: 95,711,799
[index] number of equivalence classes: 391,900
[quant] running in paired-end mode
[quant] will process pair 1: SRR33623191-trimmed-pair1.fastq
                             SRR33623191-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 24,881,013 reads, 22,154,452 reads pseudoaligned
[quant] estimated average fragment length: 253.076
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,069 rounds

 109283 SRR33623191.ke.tsv
  52636 SRR33623191.se.tsv
 161919 total
==> SRR33623191.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	17	0	0
ENSMUST00000196221.1	9	10	0	0
ENSMUST00000179664.1	11	12	0	0
ENSMUST00000178537.1	12	13	0	0
ENSMUST00000178862.1	14	15	0	0
ENSMUST00000179520.1	11	12	0	0
ENSMUST00000179883.1	16	17	0	0
ENSMUST00000195858.1	10	11	0	0
ENSMUST00000179932.1	12	13	0	0

==> SRR33623191.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	50
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	8
ENSMUSG00000103025	0
ENSMUSG00000089699	2
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR33623191 completed mapping pipeline successfully
