Starting /dee2/code/volunteer_pipeline.sh SRR34296823
    current disk space = 254745644
    free memory = 197185892 
SRR34296823 SRAfilesize
d2b722856c53b771e5015b80f5097490  SRR34296823.sra
SRR34296823.sra file validated
SRR34296823 is single end
SRR34296823 is conventional basespace
SRR34296823 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR34296823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12225	37.0	20.0	37.0	12.0	37.0
2	34.58875	37.0	37.0	37.0	20.0	37.0
3	35.2315	37.0	37.0	37.0	37.0	37.0
4	34.9945	37.0	37.0	37.0	37.0	37.0
5	35.22525	37.0	37.0	37.0	37.0	37.0
6	35.1115	37.0	37.0	37.0	37.0	37.0
7	35.803	37.0	37.0	37.0	37.0	37.0
8	35.859	37.0	37.0	37.0	37.0	37.0
9	35.7485	37.0	37.0	37.0	37.0	37.0
10	36.28975	37.0	37.0	37.0	37.0	37.0
11	36.17275	37.0	37.0	37.0	37.0	37.0
12	36.2905	37.0	37.0	37.0	37.0	37.0
13	36.2585	37.0	37.0	37.0	37.0	37.0
14	36.2875	37.0	37.0	37.0	37.0	37.0
15	36.413	37.0	37.0	37.0	37.0	37.0
16	35.991	37.0	37.0	37.0	37.0	37.0
17	36.50675	37.0	37.0	37.0	37.0	37.0
18	36.39475	37.0	37.0	37.0	37.0	37.0
19	36.34025	37.0	37.0	37.0	37.0	37.0
20	36.46475	37.0	37.0	37.0	37.0	37.0
21	36.3945	37.0	37.0	37.0	37.0	37.0
22	36.3655	37.0	37.0	37.0	37.0	37.0
23	36.3735	37.0	37.0	37.0	37.0	37.0
24	36.42625	37.0	37.0	37.0	37.0	37.0
25	36.376	37.0	37.0	37.0	37.0	37.0
26	36.118	37.0	37.0	37.0	37.0	37.0
27	36.16825	37.0	37.0	37.0	37.0	37.0
28	36.23475	37.0	37.0	37.0	37.0	37.0
29	36.303	37.0	37.0	37.0	37.0	37.0
30	36.10025	37.0	37.0	37.0	37.0	37.0
31	36.2775	37.0	37.0	37.0	37.0	37.0
32	36.11475	37.0	37.0	37.0	37.0	37.0
33	36.44625	37.0	37.0	37.0	37.0	37.0
34	36.35075	37.0	37.0	37.0	37.0	37.0
35	36.3485	37.0	37.0	37.0	37.0	37.0
36	36.24675	37.0	37.0	37.0	37.0	37.0
37	36.084	37.0	37.0	37.0	37.0	37.0
38	36.10425	37.0	37.0	37.0	37.0	37.0
39	35.615	37.0	37.0	37.0	37.0	37.0
40	36.1055	37.0	37.0	37.0	37.0	37.0
41	35.86075	37.0	37.0	37.0	37.0	37.0
42	36.24875	37.0	37.0	37.0	37.0	37.0
43	36.153	37.0	37.0	37.0	37.0	37.0
44	36.26875	37.0	37.0	37.0	37.0	37.0
45	36.01575	37.0	37.0	37.0	37.0	37.0
46	36.26325	37.0	37.0	37.0	37.0	37.0
47	36.1795	37.0	37.0	37.0	37.0	37.0
48	36.06175	37.0	37.0	37.0	37.0	37.0
49	36.10325	37.0	37.0	37.0	37.0	37.0
50	35.84025	37.0	37.0	37.0	37.0	37.0
51	36.15825	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	4.0
21	9.0
22	8.0
23	9.0
24	11.0
25	4.0
26	7.0
27	5.0
28	6.0
29	15.0
30	19.0
31	34.0
32	59.0
33	100.0
34	173.0
35	481.0
36	1837.0
37	1215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	3.2	17.775	70.975	8.05
2	26.02079634795841	19.528277960943445	14.76033477047933	39.69059092061882
3	13.425	34.949999999999996	24.125	27.500000000000004
4	24.25	24.6	18.575	32.574999999999996
5	25.874999999999996	38.45	24.975	10.7
6	45.85	14.7	22.325	17.125
7	17.575	30.75	26.950000000000003	24.725
8	36.7	23.674999999999997	22.975	16.650000000000002
9	15.125	32.375	43.65	8.85
10	13.350000000000001	44.15	15.225	27.275
11	26.35	6.175	24.625	42.85
12	20.025000000000002	13.750000000000002	18.3	47.925000000000004
13	24.6	18.075	41.3	16.025
14	34.2	33.25	20.9	11.65
15	37.225	27.575	23.1	12.1
16	9.6	19.55	25.874999999999996	44.975
17	15.950000000000001	18.15	50.775000000000006	15.125
18	28.825	11.275	28.299999999999997	31.6
19	31.775	15.1	42.199999999999996	10.925
20	32.300000000000004	19.7	34.599999999999994	13.4
21	16.075	13.075000000000001	55.55	15.299999999999999
22	28.675	14.475	52.05	4.8
23	31.825	14.799999999999999	46.050000000000004	7.324999999999999
24	60.275	11.075	26.700000000000003	1.95
25	55.7	30.125	12.174999999999999	2.0
26	26.700000000000003	58.875	11.200000000000001	3.225
27	9.325	54.325	31.45	4.9
28	4.3999999999999995	25.5	61.724999999999994	8.375
29	3.6249999999999996	8.425	61.7	26.25
30	5.4	2.8000000000000003	49.175000000000004	42.625
31	10.85	1.825	44.375	42.95
32	32.824999999999996	1.575	22.35	43.25
33	66.10000000000001	1.275	10.85	21.775
34	80.025	1.9	9.25	8.825
35	67.77499999999999	2.375	24.375	5.475
36	50.9	3.05	36.4	9.65
37	44.725	5.175	20.125	29.975
38	23.05	10.225	7.575	59.150000000000006
39	11.025	30.75	3.225	55.00000000000001
40	11.1	60.075	2.125	26.700000000000003
41	30.825000000000003	57.425000000000004	1.875	9.875
42	59.52499999999999	34.2	2.375	3.9
43	55.1	37.475	1.925	5.5
44	26.700000000000003	61.050000000000004	3.375	8.875
45	9.75	55.825	7.199999999999999	27.224999999999998
46	4.025	26.85	24.4	44.725
47	2.9250000000000003	10.875	36.625	49.575
48	4.3	9.375	20.150000000000002	66.175
49	7.475	25.35	9.049999999999999	58.12500000000001
50	24.6	37.475	8.025	29.9
51	36.675000000000004	22.675	24.55	16.1
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	1.0
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	0.5
31	0.0
32	1.0
33	2.0
34	4.0
35	6.0
36	6.0
37	6.0
38	7.0
39	8.0
40	7.0
41	6.0
42	25.0
43	44.0
44	255.0
45	466.0
46	449.5
47	433.0
48	567.5
49	702.0
50	858.0
51	1014.0
52	843.0
53	672.0
54	483.5
55	295.0
56	221.5
57	148.0
58	121.0
59	94.0
60	69.5
61	45.0
62	28.5
63	12.0
64	22.0
65	32.0
66	19.5
67	7.0
68	4.0
69	1.0
70	1.0
71	1.0
72	1.0
73	1.0
74	1.0
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.425
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10852713178294	23.9
2	10.387596899224807	6.7
3	3.3333333333333335	3.225
4	1.937984496124031	2.5
5	2.0155038759689923	3.25
6	1.317829457364341	2.55
7	0.6976744186046512	1.575
8	0.7751937984496124	2.0
9	0.31007751937984496	0.8999999999999999
>10	4.186046511627907	24.9
>50	0.6201550387596899	12.45
>100	0.31007751937984496	16.05
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	241	6.0249999999999995	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	179	4.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	121	3.025	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	101	2.5250000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	85	2.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	73	1.825	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	64	1.6	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	56	1.4000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	54	1.35	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	53	1.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	44	1.0999999999999999	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	43	1.075	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	43	1.075	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	40	1.0	RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 31bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	31	0.775	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	31	0.775	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	25	0.625	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (100% over 30bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	20	0.5	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA	19	0.475	No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	15	0.375	RNA PCR Primer, Index 1 (100% over 31bp)
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 31bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTC	13	0.325	RNA PCR Primer, Index 1 (100% over 26bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	7	0.17500000000000002	RNA PCR Primer, Index 6 (100% over 34bp)
CTTTTTGCGGTCTGGGCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGTGCTTGATCTAACCATGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ACCTTGGCTCTAGACTGCTTACTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TGGCTCAGTTCAGCAGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATTGTCTGCTGGGTTTCTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAGAGAAAGGCAGTTCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTGGTGTTGTGAATCAGGCCGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GAAGTTGTTCGTGGTGGATTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATCACATTGCCAGGGATTACCACTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ATCACATTGCCAGGGATTTCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAAT	5	0.125	No Hit
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGTGCTTGATCTAACCATGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTATTGCTTAAGAATACGCGTAGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGCTGGGGTGGAACCTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TATGGCTTTTTATTCCTATGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.05	0.0	0.0	0.0
3	0.0	0.05	0.0	0.0	0.0
4	0.0	0.1	0.0	0.0	0.0
5	0.0	0.1	0.0	0.0	0.0
6	0.0	0.1	0.0	0.0	0.0
7	0.0	0.125	0.0	0.0	0.0
8	0.0	0.175	0.0	0.0	0.0
9	0.0	0.175	0.0	0.0	0.0
10	0.0	0.175	0.0	0.0	0.0
11	0.0	0.175	0.0	0.0	0.0
12	0.0	0.175	0.0	0.0	0.0
13	0.0	0.325	0.0	0.0	0.0
14	0.0	0.475	0.0	0.0	0.0
15	0.0	0.625	0.0	0.0	0.0
16	0.0	0.825	0.0	0.0	0.0
17	0.0	1.225	0.0	0.0	0.0
18	0.0	1.85	0.0	0.0	0.0
19	0.0	2.675	0.0	0.0	0.0
20	0.0	4.825	0.0	0.0	0.0
21	0.0	10.65	0.0	0.0	0.0
22	0.0	33.05	0.0	0.0	0.0
23	0.0	67.75	0.0	0.0	0.0
24	0.0	86.25	0.0	0.0	0.0
25	0.0	92.4	0.0	0.0	0.0
26	0.0	93.75	0.0	0.0	0.0
27	0.0	94.25	0.0	0.0	0.0
28	0.0	94.7	0.0	0.0	0.0
29	0.0	94.9	0.0	0.0	0.0
30	0.0	95.1	0.0	0.0	0.0
31	0.0	95.4	0.0	0.0	0.0
32	0.0	95.575	0.0	0.0	0.0
33	0.0	95.9	0.0	0.0	0.0
34	0.0	96.575	0.0	0.0	0.0
35	0.0	96.75	0.0	0.0	0.0
36	0.0	96.8	0.0	0.0	0.0
37	0.0	96.85	0.0	0.0	0.0
38	0.0	96.975	0.0	0.0	0.0
39	0.0	97.125	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTATG	45	2.2009772E-10	45.000004	16
TGAGACC	20	6.222983E-4	45.0	5
ACAGTGA	25	3.236105E-5	45.0	3
ATCCTAC	25	3.236105E-5	45.0	8
GAGGTAG	40	4.3564796E-9	45.0	2
GTTTGTA	20	6.222983E-4	45.0	11
TGGTTAT	55	0.0	45.0	5
GTATGAT	40	4.3564796E-9	45.0	18
AACCGGT	25	3.236105E-5	45.0	9
CTGAGAC	20	6.222983E-4	45.0	4
TACAGTT	20	6.222983E-4	45.0	16
TCTAGCT	50	1.0913936E-11	45.0	11
ACTTGTG	20	6.222983E-4	45.0	15
AGTAGTT	20	6.222983E-4	45.0	7
TCCTACA	25	3.236105E-5	45.0	9
CATCCTA	25	3.236105E-5	45.0	7
CCTAACT	20	6.222983E-4	45.0	11
ATCTAGC	50	1.0913936E-11	45.0	10
TTATCTA	50	1.0913936E-11	45.0	8
GGTCTCT	25	3.236105E-5	45.0	13
>>END_MODULE
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
Read 2378637 spots for SRR34296823.sra
Written 2378637 spots for SRR34296823.sra
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
Read 2378632 spots for SRR34296823.sra
Written 2378632 spots for SRR34296823.sra
SRR ids: ['SRR34296823.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fbxac3k7
SRR34296823.sra spots: 19029061
blocks: [[1, 2378632], [2378633, 4757264], [4757265, 7135896], [7135897, 9514528], [9514529, 11893160], [11893161, 14271792], [14271793, 16650424], [16650425, 19029061]]
SRR34296823 file size 3287912
SRR34296823 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR34296823 SRR34296823_1.fastq
Input file:	SRR34296823_1.fastq
trimmed:	SRR34296823-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Thu Feb 19 09:15:29 2026 >> started

Thu Feb 19 09:15:37 2026 >> done (8.109s)
19029061 reads processed; of these:
       1 ( 0.00%) short reads filtered out after trimming by size control
     872 ( 0.00%) empty reads filtered out after trimming by size control
19028188 (100.00%) reads available; of these:
19028188 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 51	19028188	100.00%
19028188 reads passed initial QC


criterion=sequence-density
sequence-density=97.24
sequence-density-rank=1
fanout-score=17.22
fanout-score-rank=1
prefix-density=97.45
prefix-fanout=17.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTCATATCT


criterion=fanout-score
sequence-density=97.24
sequence-density-rank=1
fanout-score=17.22
fanout-score-rank=1
prefix-density=97.45
prefix-fanout=17.2
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTCATATCT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTCATATCT -o SRR34296823 -
Input file:	STDIN
trimmed:	SRR34296823-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCTCATATCT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Thu Feb 19 09:15:57 2026 >> started

Thu Feb 19 09:16:13 2026 >> done (15.657s)
18639858 reads processed; of these:
  367191 ( 1.97%) short reads filtered out after trimming by size control
    5611 ( 0.03%) empty reads filtered out after trimming by size control
18267056 (98.00%) reads available; of these:
18105943 (99.12%) trimmed reads available after processing
  161113 ( 0.88%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  157957	  0.86%
 19	  448864	  2.46%
 20	 1061276	  5.81%
 21	 4161303	 22.78%
 22	 6595628	 36.11%
 23	 3444762	 18.86%
 24	 1186247	  6.49%
 25	  298209	  1.63%
 26	  113365	  0.62%
 27	   82355	  0.45%
 28	   67462	  0.37%
 29	   43605	  0.24%
 30	   39365	  0.22%
 31	   51134	  0.28%
 32	   67736	  0.37%
 33	   98515	  0.54%
 34	   28612	  0.16%
 35	   18439	  0.10%
 36	   15139	  0.08%
 37	   19128	  0.10%
 38	   11432	  0.06%
 39	    9468	  0.05%
 40	   12300	  0.07%
 41	   10068	  0.06%
 42	    9460	  0.05%
 43	    9963	  0.05%
 44	   11178	  0.06%
 45	   21726	  0.12%
 46	    4224	  0.02%
 47	    2605	  0.01%
 48	    4418	  0.02%
 49	       0	  0.00%
 50	       0	  0.00%
 51	  161113	  0.88%


criterion=sequence-density
sequence-density=9.19
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=9
prefix-density=0.00
prefix-fanout=1.0
sequence=TCTTTGGTTATCTAGCTGTATGAAA


criterion=fanout-score
sequence-density=0.71
sequence-density-rank=19
fanout-score=7.84
fanout-score-rank=1
prefix-density=5.50
prefix-fanout=1.0
sequence=GTAGTAGGTTGCATAGTTT
                                 Started job on |	Feb 19 09:16:24
                             Started mapping on |	Feb 19 09:16:24
                                    Finished on |	Feb 19 09:17:24
       Mapping speed, Million of reads per hour |	1119.32

                          Number of input reads |	18655386
                      Average input read length |	23
                                    UNIQUE READS:
                   Uniquely mapped reads number |	8708939
                        Uniquely mapped reads % |	46.68%
                          Average mapped length |	22.29
                       Number of splices: Total |	9172
            Number of splices: Annotated (sjdb) |	1740
                       Number of splices: GT/AG |	8995
                       Number of splices: GC/AG |	93
                       Number of splices: AT/AC |	5
               Number of splices: Non-canonical |	79
                      Mismatch rate per base, % |	0.14%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.32
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.10
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	9059827
             % of reads mapped to multiple loci |	48.56%
        Number of reads mapped to too many loci |	106075
             % of reads mapped to too many loci |	0.57%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.60%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	886620	886620	886620
N_multimapping	9059827	9059827	9059827
N_noFeature	288470	313209	7151492
N_ambiguous	3124876	1836953	34039
UnstrandedReadsAssigned:5295593 PositiveStrandReadsAssigned:6558777 NegativeStrandReadsAssigned:1523408
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR34296823 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR34296823-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 18,655,386 reads, 2,061,189 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 970 rounds

 109283 SRR34296823.ke.tsv
  52636 SRR34296823.se.tsv
 161919 total
==> SRR34296823.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR34296823.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	1
ENSMUSG00000051951	5
ENSMUSG00000102851	0
ENSMUSG00000103377	1
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR34296823 completed mapping pipeline successfully
