Starting /dee2/code/volunteer_pipeline.sh SRR34296801
    current disk space = 226460272
    free memory = 196541492 
SRR34296801 SRAfilesize
b200adc7d93a327b2e960debb0fdc0a4  SRR34296801.sra
SRR34296801.sra file validated
SRR34296801 is single end
SRR34296801 is conventional basespace
SRR34296801 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR34296801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91475	37.0	37.0	37.0	20.0	37.0
2	34.8435	37.0	37.0	37.0	20.0	37.0
3	35.16925	37.0	37.0	37.0	37.0	37.0
4	35.594	37.0	37.0	37.0	37.0	37.0
5	35.95475	37.0	37.0	37.0	37.0	37.0
6	35.9435	37.0	37.0	37.0	37.0	37.0
7	36.078	37.0	37.0	37.0	37.0	37.0
8	36.3205	37.0	37.0	37.0	37.0	37.0
9	35.89225	37.0	37.0	37.0	37.0	37.0
10	36.2725	37.0	37.0	37.0	37.0	37.0
11	36.52475	37.0	37.0	37.0	37.0	37.0
12	36.44775	37.0	37.0	37.0	37.0	37.0
13	36.4765	37.0	37.0	37.0	37.0	37.0
14	36.259	37.0	37.0	37.0	37.0	37.0
15	36.409	37.0	37.0	37.0	37.0	37.0
16	36.49075	37.0	37.0	37.0	37.0	37.0
17	36.576	37.0	37.0	37.0	37.0	37.0
18	36.54075	37.0	37.0	37.0	37.0	37.0
19	36.68025	37.0	37.0	37.0	37.0	37.0
20	36.62825	37.0	37.0	37.0	37.0	37.0
21	36.69325	37.0	37.0	37.0	37.0	37.0
22	36.49875	37.0	37.0	37.0	37.0	37.0
23	36.5615	37.0	37.0	37.0	37.0	37.0
24	36.6615	37.0	37.0	37.0	37.0	37.0
25	36.6725	37.0	37.0	37.0	37.0	37.0
26	36.4325	37.0	37.0	37.0	37.0	37.0
27	36.414	37.0	37.0	37.0	37.0	37.0
28	36.28475	37.0	37.0	37.0	37.0	37.0
29	36.43275	37.0	37.0	37.0	37.0	37.0
30	36.589	37.0	37.0	37.0	37.0	37.0
31	36.52275	37.0	37.0	37.0	37.0	37.0
32	36.55375	37.0	37.0	37.0	37.0	37.0
33	36.656	37.0	37.0	37.0	37.0	37.0
34	36.708	37.0	37.0	37.0	37.0	37.0
35	36.5745	37.0	37.0	37.0	37.0	37.0
36	36.6375	37.0	37.0	37.0	37.0	37.0
37	36.55375	37.0	37.0	37.0	37.0	37.0
38	36.18425	37.0	37.0	37.0	37.0	37.0
39	36.294	37.0	37.0	37.0	37.0	37.0
40	36.3405	37.0	37.0	37.0	37.0	37.0
41	36.29	37.0	37.0	37.0	37.0	37.0
42	36.42275	37.0	37.0	37.0	37.0	37.0
43	36.52925	37.0	37.0	37.0	37.0	37.0
44	36.586	37.0	37.0	37.0	37.0	37.0
45	36.43175	37.0	37.0	37.0	37.0	37.0
46	36.49325	37.0	37.0	37.0	37.0	37.0
47	36.407	37.0	37.0	37.0	37.0	37.0
48	36.39575	37.0	37.0	37.0	37.0	37.0
49	36.2275	37.0	37.0	37.0	37.0	37.0
50	36.2895	37.0	37.0	37.0	37.0	37.0
51	36.437	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	6.0
22	2.0
23	3.0
24	6.0
25	4.0
26	5.0
27	3.0
28	5.0
29	6.0
30	13.0
31	22.0
32	36.0
33	70.0
34	122.0
35	324.0
36	1708.0
37	1661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.375	14.899999999999999	75.925	6.800000000000001
2	25.950163604329223	19.456330229046063	15.001258494840172	39.59224767178455
3	12.925	38.175	25.3	23.599999999999998
4	25.275	24.5	16.950000000000003	33.275
5	25.75	41.625	23.45	9.175
6	46.550000000000004	14.124999999999998	23.125	16.2
7	17.525	31.8	27.175	23.5
8	38.625	23.5	21.05	16.825000000000003
9	14.6	32.475	44.224999999999994	8.7
10	12.375	46.6	14.875	26.150000000000002
11	26.55	5.949999999999999	23.025000000000002	44.474999999999994
12	19.725	13.5	19.625	47.15
13	26.075	17.875	40.8	15.25
14	34.575	31.374999999999996	22.3	11.75
15	38.15	26.55	23.625	11.675
16	8.55	19.85	25.4	46.2
17	13.775	19.025	53.65	13.55
18	29.325000000000003	11.275	27.250000000000004	32.15
19	30.825000000000003	16.1	41.55	11.525
20	32.4	19.3	35.425000000000004	12.875
21	14.725	12.55	56.8	15.925
22	29.425	14.299999999999999	52.025	4.25
23	31.275	14.575	47.025	7.124999999999999
24	60.375	11.05	26.6	1.975
25	56.05	29.925	12.25	1.775
26	26.474999999999998	59.975	11.475	2.075
27	9.325	55.300000000000004	31.4	3.975
28	4.05	25.2	63.05	7.7
29	3.375	8.0	62.4	26.224999999999998
30	5.4	2.375	48.55	43.675000000000004
31	11.075	1.4000000000000001	45.074999999999996	42.449999999999996
32	32.75	1.325	21.775	44.15
33	67.0	1.4000000000000001	10.625	20.974999999999998
34	81.39999999999999	1.625	8.35	8.625
35	68.77499999999999	1.725	24.125	5.375
36	50.425	2.375	37.175000000000004	10.025
37	46.0	5.0	19.275000000000002	29.725
38	22.275	10.475	6.9750000000000005	60.275
39	11.4	30.3	2.325	55.974999999999994
40	10.975	60.724999999999994	1.725	26.575
41	30.525000000000002	58.875	1.175	9.425
42	60.0	34.449999999999996	1.35	4.2
43	56.05	37.525	1.4000000000000001	5.025
44	26.125	61.95	3.375	8.55
45	8.975	56.574999999999996	7.025	27.425
46	3.1	26.700000000000003	23.724999999999998	46.475
47	2.15	11.600000000000001	37.5	48.75
48	3.775	9.45	19.925	66.85
49	6.875000000000001	24.775	9.8	58.550000000000004
50	23.175	38.725	8.475000000000001	29.625
51	36.775000000000006	23.225	24.125	15.875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.0
26	1.0
27	2.0
28	1.0
29	0.0
30	0.0
31	0.0
32	1.0
33	2.0
34	1.5
35	1.0
36	3.0
37	5.0
38	5.0
39	5.0
40	4.5
41	4.0
42	25.5
43	47.0
44	276.0
45	505.0
46	485.5
47	466.0
48	596.5
49	727.0
50	852.5
51	978.0
52	832.5
53	687.0
54	486.5
55	286.0
56	217.0
57	148.0
58	107.5
59	67.0
60	52.5
61	38.0
62	23.5
63	9.0
64	14.0
65	19.0
66	10.5
67	2.0
68	1.0
69	0.0
70	0.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.675
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.775000000000002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.41789748045178	19.975
2	12.510860121633364	7.199999999999999
3	4.691572545612511	4.05
4	2.519548218940052	2.9000000000000004
5	1.9113814074717639	2.75
6	1.2163336229365769	2.1
7	0.7819287576020852	1.575
8	0.6081668114682884	1.4000000000000001
9	0.5212858384013901	1.35
>10	4.8653344917463075	26.900000000000002
>50	0.6081668114682884	12.55
>100	0.3475238922675934	17.25
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	275	6.875000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	175	4.375	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	138	3.45	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	102	2.55	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	100	2.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	90	2.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	71	1.775	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	69	1.725	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	64	1.6	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	53	1.325	RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	49	1.225	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	49	1.225	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	47	1.175	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	30	0.75	RNA PCR Primer, Index 1 (100% over 31bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	25	0.625	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	24	0.6	RNA PCR Primer, Index 1 (100% over 31bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 31bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	20	0.5	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 31bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TCTCACACAGAAATCGCACCCGTCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TAAAGCTAGATAACCGAAAGTATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TACGTCATCGTCGTCATCGTTATTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCAATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTAACTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CTTCTCACTACTGCACTTGACTAGTCTTTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCCGGCATCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TGAGGTAGTAGATTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TTAAGGCACGCGGTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
TAGCACCATCTGAAATCGGTTATTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.1	0.0	0.0	0.0
14	0.0	0.225	0.0	0.0	0.0
15	0.0	0.5	0.0	0.0	0.0
16	0.0	0.95	0.0	0.0	0.0
17	0.0	1.45	0.0	0.0	0.0
18	0.0	1.925	0.0	0.0	0.0
19	0.0	2.875	0.0	0.0	0.0
20	0.0	5.575	0.0	0.0	0.0
21	0.0	11.625	0.0	0.0	0.0
22	0.0	34.075	0.0	0.0	0.0
23	0.0	70.125	0.0	0.0	0.0
24	0.0	88.525	0.0	0.0	0.0
25	0.0	94.85	0.0	0.0	0.0
26	0.0	96.15	0.0	0.0	0.0
27	0.0	96.825	0.0	0.0	0.0
28	0.0	97.025	0.0	0.0	0.0
29	0.0	97.325	0.0	0.0	0.0
30	0.0	97.425	0.0	0.0	0.0
31	0.0	97.575	0.0	0.0	0.0
32	0.0	97.675	0.0	0.0	0.0
33	0.0	97.825	0.0	0.0	0.0
34	0.0	98.125	0.0	0.0	0.0
35	0.0	98.175	0.0	0.0	0.0
36	0.0	98.225	0.0	0.0	0.0
37	0.0	98.25	0.0	0.0	0.0
38	0.0	98.25	0.0	0.0	0.0
39	0.0	98.3	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGA	45	2.2009772E-10	45.000004	3
ACCGGTC	45	2.2009772E-10	45.000004	10
AACCGGT	45	2.2009772E-10	45.000004	9
GGTCTCT	45	2.2009772E-10	45.000004	13
CTCTTTT	45	2.2009772E-10	45.000004	16
GAACCGG	45	2.2009772E-10	45.000004	8
GTCTCTT	45	2.2009772E-10	45.000004	14
AGTGAAC	45	2.2009772E-10	45.000004	5
TCTCTTT	45	2.2009772E-10	45.000004	15
GTGAACC	45	2.2009772E-10	45.000004	6
CGGTCTC	45	2.2009772E-10	45.000004	12
TGAACCG	45	2.2009772E-10	45.000004	7
CAGTGAA	45	2.2009772E-10	45.000004	4
CCGGTCT	45	2.2009772E-10	45.000004	11
GCCCTAG	20	6.222983E-4	45.0	11
ATCCCCG	25	3.236105E-5	45.0	8
ATCCTAG	20	6.222983E-4	45.0	5
ACTAATC	20	6.222983E-4	45.0	1
GAGGTAG	35	8.569259E-8	45.0	2
AATCCTA	20	6.222983E-4	45.0	4
>>END_MODULE
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903000 spots for SRR34296801.sra
Written 1903000 spots for SRR34296801.sra
Read 1903001 spots for SRR34296801.sra
Written 1903001 spots for SRR34296801.sra
SRR ids: ['SRR34296801.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_vvapd8i3
SRR34296801.sra spots: 15224001
blocks: [[1, 1903000], [1903001, 3806000], [3806001, 5709000], [5709001, 7612000], [7612001, 9515000], [9515001, 11418000], [11418001, 13321000], [13321001, 15224001]]
SRR34296801 file size 2628280
SRR34296801 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR34296801 SRR34296801_1.fastq
Input file:	SRR34296801_1.fastq
trimmed:	SRR34296801-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Thu Feb 19 09:26:04 2026 >> started

Thu Feb 19 09:26:09 2026 >> done (4.383s)
15224001 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
      99 ( 0.00%) empty reads filtered out after trimming by size control
15223902 (100.00%) reads available; of these:
     301 ( 0.00%) trimmed reads available after processing
15223601 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 50	     301	  0.00%
 51	15223601	100.00%
15223902 reads passed initial QC


criterion=sequence-density
sequence-density=98.29
sequence-density-rank=1
fanout-score=16.98
fanout-score-rank=1
prefix-density=98.52
prefix-fanout=16.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACATCATCT


criterion=fanout-score
sequence-density=98.29
sequence-density-rank=1
fanout-score=16.98
fanout-score-rank=1
prefix-density=98.52
prefix-fanout=16.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACATCATCT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACATCATCT -o SRR34296801 -
Input file:	STDIN
trimmed:	SRR34296801-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTACATCATCT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Thu Feb 19 09:26:21 2026 >> started

Thu Feb 19 09:26:29 2026 >> done (8.234s)
14916349 reads processed; of these:
  320220 ( 2.15%) short reads filtered out after trimming by size control
    1625 ( 0.01%) empty reads filtered out after trimming by size control
14594504 (97.84%) reads available; of these:
14540630 (99.63%) trimmed reads available after processing
   53874 ( 0.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  148915	  1.02%
 19	  428238	  2.93%
 20	  950008	  6.51%
 21	 3672392	 25.16%
 22	 5333212	 36.54%
 23	 2673576	 18.32%
 24	  853392	  5.85%
 25	  187021	  1.28%
 26	   61945	  0.42%
 27	   38643	  0.26%
 28	   26615	  0.18%
 29	   15270	  0.10%
 30	   12594	  0.09%
 31	   17051	  0.12%
 32	   22333	  0.15%
 33	   35541	  0.24%
 34	   11036	  0.08%
 35	    7427	  0.05%
 36	    6527	  0.04%
 37	    7757	  0.05%
 38	    4572	  0.03%
 39	    3427	  0.02%
 40	    4109	  0.03%
 41	    3313	  0.02%
 42	    2836	  0.02%
 43	    2680	  0.02%
 44	    2728	  0.02%
 45	    4580	  0.03%
 46	     768	  0.01%
 47	     506	  0.00%
 48	    1618	  0.01%
 49	       0	  0.00%
 50	       0	  0.00%
 51	   53874	  0.37%


criterion=sequence-density
sequence-density=10.10
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=8
prefix-density=0.00
prefix-fanout=1.0
sequence=TCTTTGGTTATCTAGCTGTATGAAA


criterion=fanout-score
sequence-density=0.65
sequence-density-rank=23
fanout-score=7.61
fanout-score-rank=1
prefix-density=4.91
prefix-fanout=1.0
sequence=GTAGTAGGTTGCATAGTTT
                                 Started job on |	Feb 19 09:26:37
                             Started mapping on |	Feb 19 09:26:37
                                    Finished on |	Feb 19 09:27:36
       Mapping speed, Million of reads per hour |	909.28

                          Number of input reads |	14902057
                      Average input read length |	22
                                    UNIQUE READS:
                   Uniquely mapped reads number |	6985263
                        Uniquely mapped reads % |	46.87%
                          Average mapped length |	21.98
                       Number of splices: Total |	5532
            Number of splices: Annotated (sjdb) |	1931
                       Number of splices: GT/AG |	5437
                       Number of splices: GC/AG |	73
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	21
                      Mismatch rate per base, % |	0.10%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.30
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7404668
             % of reads mapped to multiple loci |	49.69%
        Number of reads mapped to too many loci |	50806
             % of reads mapped to too many loci |	0.34%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.74%
                     % of reads unmapped: other |	0.36%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	512126	512126	512126
N_multimapping	7404668	7404668	7404668
N_noFeature	176265	191375	5710736
N_ambiguous	2525610	1475990	34523
UnstrandedReadsAssigned:4283388 PositiveStrandReadsAssigned:5317898 NegativeStrandReadsAssigned:1240004
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR34296801 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR34296801-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 14,902,057 reads, 1,632,600 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 956 rounds

 109283 SRR34296801.ke.tsv
  52636 SRR34296801.se.tsv
 161919 total
==> SRR34296801.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR34296801.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	5
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR34296801 completed mapping pipeline successfully
