Starting /dee2/code/volunteer_pipeline.sh SRR34296833
    current disk space = 269675600
    free memory = 201679076 
SRR34296833 SRAfilesize
d203785e285c9b7e2e44bf869e3ff882  SRR34296833.sra
SRR34296833.sra file validated
SRR34296833 is single end
SRR34296833 is conventional basespace
SRR34296833 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR34296833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.975	37.0	37.0	37.0	12.0	37.0
2	34.27125	37.0	37.0	37.0	20.0	37.0
3	35.15325	37.0	37.0	37.0	37.0	37.0
4	35.11775	37.0	37.0	37.0	37.0	37.0
5	35.008	37.0	37.0	37.0	37.0	37.0
6	35.39775	37.0	37.0	37.0	37.0	37.0
7	35.8765	37.0	37.0	37.0	37.0	37.0
8	36.02675	37.0	37.0	37.0	37.0	37.0
9	35.9545	37.0	37.0	37.0	37.0	37.0
10	36.28575	37.0	37.0	37.0	37.0	37.0
11	36.132	37.0	37.0	37.0	37.0	37.0
12	36.332	37.0	37.0	37.0	37.0	37.0
13	36.29	37.0	37.0	37.0	37.0	37.0
14	36.191	37.0	37.0	37.0	37.0	37.0
15	36.543	37.0	37.0	37.0	37.0	37.0
16	36.09925	37.0	37.0	37.0	37.0	37.0
17	36.55475	37.0	37.0	37.0	37.0	37.0
18	36.58825	37.0	37.0	37.0	37.0	37.0
19	36.39625	37.0	37.0	37.0	37.0	37.0
20	36.474	37.0	37.0	37.0	37.0	37.0
21	36.4555	37.0	37.0	37.0	37.0	37.0
22	36.443	37.0	37.0	37.0	37.0	37.0
23	36.52575	37.0	37.0	37.0	37.0	37.0
24	36.41775	37.0	37.0	37.0	37.0	37.0
25	36.47175	37.0	37.0	37.0	37.0	37.0
26	36.1895	37.0	37.0	37.0	37.0	37.0
27	36.186	37.0	37.0	37.0	37.0	37.0
28	36.20275	37.0	37.0	37.0	37.0	37.0
29	36.28525	37.0	37.0	37.0	37.0	37.0
30	36.1545	37.0	37.0	37.0	37.0	37.0
31	36.40725	37.0	37.0	37.0	37.0	37.0
32	36.195	37.0	37.0	37.0	37.0	37.0
33	36.46125	37.0	37.0	37.0	37.0	37.0
34	36.4175	37.0	37.0	37.0	37.0	37.0
35	36.37375	37.0	37.0	37.0	37.0	37.0
36	36.357	37.0	37.0	37.0	37.0	37.0
37	36.307	37.0	37.0	37.0	37.0	37.0
38	36.2315	37.0	37.0	37.0	37.0	37.0
39	35.87825	37.0	37.0	37.0	37.0	37.0
40	36.0975	37.0	37.0	37.0	37.0	37.0
41	36.022	37.0	37.0	37.0	37.0	37.0
42	36.24275	37.0	37.0	37.0	37.0	37.0
43	36.17075	37.0	37.0	37.0	37.0	37.0
44	36.29425	37.0	37.0	37.0	37.0	37.0
45	36.09825	37.0	37.0	37.0	37.0	37.0
46	36.297	37.0	37.0	37.0	37.0	37.0
47	36.33175	37.0	37.0	37.0	37.0	37.0
48	36.233	37.0	37.0	37.0	37.0	37.0
49	36.177	37.0	37.0	37.0	37.0	37.0
50	35.914	37.0	37.0	37.0	37.0	37.0
51	36.34325	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	7.0
22	5.0
23	11.0
24	4.0
25	9.0
26	8.0
27	6.0
28	9.0
29	8.0
30	13.0
31	21.0
32	56.0
33	76.0
34	196.0
35	508.0
36	1786.0
37	1276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.05	16.55	70.6	8.799999999999999
2	26.017925736235597	17.772087067861715	15.108834827144687	41.101152368758
3	13.225000000000001	36.675000000000004	23.775	26.325
4	25.324999999999996	22.6	18.525	33.550000000000004
5	27.125	36.975	25.324999999999996	10.575
6	47.675	13.5	23.425	15.4
7	19.475	31.175000000000004	27.750000000000004	21.6
8	39.675	20.8	23.599999999999998	15.925
9	15.25	32.574999999999996	41.725	10.45
10	15.024999999999999	46.025	14.725	24.224999999999998
11	26.825	5.5	24.75	42.925000000000004
12	20.925	13.525	18.224999999999998	47.325
13	27.825	15.950000000000001	41.0	15.225
14	33.725	31.7	22.175	12.4
15	38.975	24.075	24.8	12.15
16	9.875	18.15	26.075	45.9
17	16.5	18.2	51.9	13.4
18	29.175	10.5	28.725	31.6
19	30.825000000000003	15.6	43.2	10.375
20	32.175	20.625	35.75	11.450000000000001
21	16.625	13.325000000000001	55.825	14.224999999999998
22	28.050000000000004	14.499999999999998	52.275000000000006	5.175
23	30.8	15.75	46.025	7.425
24	58.650000000000006	10.424999999999999	28.000000000000004	2.9250000000000003
25	55.800000000000004	28.325	12.975	2.9000000000000004
26	27.425	57.225	11.450000000000001	3.9
27	10.05	54.300000000000004	30.55	5.1
28	6.075	25.424999999999997	60.25	8.25
29	5.825	8.35	60.875	24.95
30	7.1	3.35	47.9	41.65
31	11.575000000000001	2.1999999999999997	43.75	42.475
32	31.65	2.025	23.575	42.75
33	64.55	2.225	10.6	22.625
34	79.10000000000001	2.625	8.5	9.775
35	66.9	2.825	23.525	6.75
36	50.125	3.925	36.25	9.700000000000001
37	44.9	6.15	20.175	28.775000000000002
38	24.775	10.2	7.000000000000001	58.025000000000006
39	12.125	29.725	2.9250000000000003	55.225
40	10.95	59.325	2.45	27.275
41	29.099999999999998	58.12500000000001	2.4	10.375
42	57.925000000000004	33.7	2.125	6.25
43	55.125	36.1	2.375	6.4
44	26.700000000000003	59.85	3.4750000000000005	9.975000000000001
45	9.275	56.625	6.9	27.200000000000003
46	4.125	28.249999999999996	23.225	44.4
47	3.775	11.600000000000001	36.225	48.4
48	4.15	9.775	21.05	65.025
49	6.7	25.974999999999998	8.725	58.599999999999994
50	22.575	39.050000000000004	7.675	30.7
51	35.625	24.474999999999998	23.525	16.375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	1.0
28	1.0
29	1.0
30	1.0
31	1.0
32	1.5
33	2.0
34	2.0
35	2.0
36	1.0
37	0.0
38	2.5
39	5.0
40	8.0
41	11.0
42	31.5
43	52.0
44	263.0
45	474.0
46	461.5
47	449.0
48	545.5
49	642.0
50	811.0
51	980.0
52	831.5
53	683.0
54	484.5
55	286.0
56	238.5
57	191.0
58	146.5
59	102.0
60	82.5
61	63.0
62	39.5
63	16.0
64	20.5
65	25.0
66	17.0
67	9.0
68	6.5
69	4.0
70	2.0
71	0.0
72	0.5
73	1.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	2.375
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.65
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.507713884993	27.275
2	9.326788218793828	6.65
3	3.7868162692847123	4.05
4	2.664796633941094	3.8
5	0.8415147265077139	1.5
6	0.8415147265077139	1.7999999999999998
7	0.9116409537166901	2.275
8	0.7012622720897616	2.0
9	0.42075736325385693	1.35
>10	3.2959326788218792	22.400000000000002
>50	0.4908835904628331	12.7
>100	0.21037868162692847	14.2
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	241	6.0249999999999995	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	180	4.5	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	147	3.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	94	2.35	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	90	2.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	85	2.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	63	1.575	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	53	1.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	53	1.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	40	1.0	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	40	1.0	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	38	0.95	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	32	0.8	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	31	0.775	RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	31	0.775	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	24	0.6	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	23	0.575	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	20	0.5	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	15	0.375	RNA PCR Primer, Index 1 (100% over 31bp)
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	15	0.375	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATCATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TNTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTAATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TNACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGAATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
ACCTTGGCTCTAGACTGCTTACTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGATCAGAAGGTGACTGTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TNACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAAAGCTAGATAACCGAAAGTATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CTAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCTCGACTCATGGTTTGAACCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.025	0.0	0.0	0.0
8	0.0	0.125	0.0	0.0	0.0
9	0.0	0.175	0.0	0.0	0.0
10	0.0	0.25	0.0	0.0	0.0
11	0.0	0.35	0.0	0.0	0.0
12	0.0	0.625	0.0	0.0	0.0
13	0.0	0.75	0.0	0.0	0.0
14	0.0	0.975	0.0	0.0	0.0
15	0.0	1.35	0.0	0.0	0.0
16	0.0	2.025	0.0	0.0	0.0
17	0.0	2.8	0.0	0.0	0.0
18	0.0	3.775	0.0	0.0	0.0
19	0.0	5.3	0.0	0.0	0.0
20	0.0	7.625	0.0	0.0	0.0
21	0.0	12.925	0.0	0.0	0.0
22	0.0	34.175	0.0	0.0	0.0
23	0.0	68.15	0.0	0.0	0.0
24	0.0	86.95	0.0	0.0	0.0
25	0.0	92.8	0.0	0.0	0.0
26	0.0	94.2	0.0	0.0	0.0
27	0.0	95.05	0.0	0.0	0.0
28	0.0	95.6	0.0	0.0	0.0
29	0.0	95.9	0.0	0.0	0.0
30	0.0	96.0	0.0	0.0	0.0
31	0.0	96.25	0.0	0.0	0.0
32	0.0	96.425	0.0	0.0	0.0
33	0.0	96.725	0.0	0.0	0.0
34	0.0	97.05	0.0	0.0	0.0
35	0.0	97.1	0.0	0.0	0.0
36	0.0	97.2	0.0	0.0	0.0
37	0.0	97.3	0.0	0.0	0.0
38	0.0	97.4	0.0	0.0	0.0
39	0.0	97.425	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGGTA	25	2.5878104E-5	46.675327	1
GAGGTAG	30	1.2856053E-6	46.675323	2
TCAAGTA	20	5.1710795E-4	46.675323	2
TTCAAGT	20	5.1710795E-4	46.675323	1
CCCTGAG	30	1.2856053E-6	46.675323	2
TCACAGT	45	1.4915713E-10	46.675323	1
CACAGTG	45	1.4915713E-10	46.675323	2
TCCCTGA	30	1.2856053E-6	46.675323	1
CCTAACT	30	1.6908471E-6	44.925003	11
TAACTTG	30	1.6908471E-6	44.925003	13
CTAACTT	30	1.6908471E-6	44.925003	12
ACCCTAA	30	1.6908471E-6	44.925003	9
GACCCTA	30	1.6908471E-6	44.925003	8
AGGTAGT	30	1.6908471E-6	44.925003	3
CCCTAAC	30	1.6908471E-6	44.925003	10
TGAGACC	40	4.4219632E-9	44.925	5
CTTTTGG	35	8.682582E-8	44.925	18
ACAGTGA	50	1.0913936E-11	44.925	3
ACCGGTC	55	0.0	44.925	10
AAGTAAT	20	6.274375E-4	44.925	4
>>END_MODULE
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
Read 3202911 spots for SRR34296833.sra
Written 3202911 spots for SRR34296833.sra
Read 3202906 spots for SRR34296833.sra
Written 3202906 spots for SRR34296833.sra
SRR ids: ['SRR34296833.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_hkpmfbwt
SRR34296833.sra spots: 25623253
blocks: [[1, 3202906], [3202907, 6405812], [6405813, 9608718], [9608719, 12811624], [12811625, 16014530], [16014531, 19217436], [19217437, 22420342], [22420343, 25623253]]
SRR34296833 file size 4431064
SRR34296833 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR34296833 SRR34296833_1.fastq
Input file:	SRR34296833_1.fastq
trimmed:	SRR34296833-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Thu Feb 19 09:04:09 2026 >> started

Thu Feb 19 09:04:14 2026 >> done (5.147s)
25623253 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
     150 ( 0.00%) empty reads filtered out after trimming by size control
25623103 (100.00%) reads available; of these:
25623103 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 51	25623103	100.00%
25623103 reads passed initial QC


criterion=sequence-density
sequence-density=97.87
sequence-density-rank=1
fanout-score=16.94
fanout-score-rank=1
prefix-density=98.12
prefix-fanout=16.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACATGCATCTGGGG


criterion=fanout-score
sequence-density=97.87
sequence-density-rank=1
fanout-score=16.94
fanout-score-rank=1
prefix-density=98.12
prefix-fanout=16.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACATGCATCTGGGG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACATGCATCTGGGG -o SRR34296833 -
Input file:	STDIN
trimmed:	SRR34296833-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACATGCATCTGGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Thu Feb 19 09:04:36 2026 >> started

Thu Feb 19 09:04:46 2026 >> done (9.889s)
25100183 reads processed; of these:
  982604 ( 3.91%) short reads filtered out after trimming by size control
    2135 ( 0.01%) empty reads filtered out after trimming by size control
24115444 (96.08%) reads available; of these:
24024616 (99.62%) trimmed reads available after processing
   90828 ( 0.38%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  389482	  1.62%
 19	  643265	  2.67%
 20	 1437016	  5.96%
 21	 5499239	 22.80%
 22	 8641758	 35.83%
 23	 4660629	 19.33%
 24	 1512217	  6.27%
 25	  423924	  1.76%
 26	  183767	  0.76%
 27	  124646	  0.52%
 28	   86667	  0.36%
 29	   52402	  0.22%
 30	   40075	  0.17%
 31	   43435	  0.18%
 32	   53756	  0.22%
 33	   80115	  0.33%
 34	   26109	  0.11%
 35	   19266	  0.08%
 36	   16102	  0.07%
 37	   16016	  0.07%
 38	   10759	  0.04%
 39	    8911	  0.04%
 40	   10867	  0.05%
 41	    8192	  0.03%
 42	    6523	  0.03%
 43	    6239	  0.03%
 44	    6450	  0.03%
 45	   11173	  0.05%
 46	    2240	  0.01%
 47	    1294	  0.01%
 48	    2082	  0.01%
 49	       0	  0.00%
 50	       0	  0.00%
 51	   90828	  0.38%


criterion=sequence-density
sequence-density=10.39
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=9
prefix-density=0.00
prefix-fanout=1.0
sequence=TCTTTGGTTATCTAGCTGTATGAAA


criterion=fanout-score
sequence-density=0.27
sequence-density-rank=39
fanout-score=49.98
fanout-score-rank=1
prefix-density=13.42
prefix-fanout=1.0
sequence=CCGGTCTCTTTAAA
                                 Started job on |	Feb 19 09:04:56
                             Started mapping on |	Feb 19 09:04:56
                                    Finished on |	Feb 19 09:06:11
       Mapping speed, Million of reads per hour |	1182.64

                          Number of input reads |	24638364
                      Average input read length |	22
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10990431
                        Uniquely mapped reads % |	44.61%
                          Average mapped length |	22.04
                       Number of splices: Total |	19887
            Number of splices: Annotated (sjdb) |	4878
                       Number of splices: GT/AG |	19573
                       Number of splices: GC/AG |	235
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	78
                      Mismatch rate per base, % |	0.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.43
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	12301578
             % of reads mapped to multiple loci |	49.93%
        Number of reads mapped to too many loci |	202383
             % of reads mapped to too many loci |	0.82%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.27%
                     % of reads unmapped: other |	1.37%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1346355	1346355	1346355
N_multimapping	12301578	12301578	12301578
N_noFeature	473496	517956	9076026
N_ambiguous	4025509	2445471	60696
UnstrandedReadsAssigned:6491426 PositiveStrandReadsAssigned:8027004 NegativeStrandReadsAssigned:1853709
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR34296833 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR34296833-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 24,638,364 reads, 2,849,097 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 970 rounds

 109283 SRR34296833.ke.tsv
  52636 SRR34296833.se.tsv
 161919 total
==> SRR34296833.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR34296833.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	17
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR34296833 completed mapping pipeline successfully
