Starting /dee2/code/volunteer_pipeline.sh SRR34296799
    current disk space = 249582176
    free memory = 195131712 
SRR34296799 SRAfilesize
0044487c88d3331a5c2254b8077dfe1b  SRR34296799.sra
SRR34296799.sra file validated
SRR34296799 is single end
SRR34296799 is conventional basespace
SRR34296799 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR34296799_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77775	37.0	37.0	37.0	20.0	37.0
2	34.66175	37.0	37.0	37.0	20.0	37.0
3	35.37325	37.0	37.0	37.0	37.0	37.0
4	35.69825	37.0	37.0	37.0	37.0	37.0
5	35.77975	37.0	37.0	37.0	37.0	37.0
6	36.101	37.0	37.0	37.0	37.0	37.0
7	36.20025	37.0	37.0	37.0	37.0	37.0
8	36.249	37.0	37.0	37.0	37.0	37.0
9	35.79425	37.0	37.0	37.0	37.0	37.0
10	36.26325	37.0	37.0	37.0	37.0	37.0
11	36.53775	37.0	37.0	37.0	37.0	37.0
12	36.178	37.0	37.0	37.0	37.0	37.0
13	36.2725	37.0	37.0	37.0	37.0	37.0
14	36.33375	37.0	37.0	37.0	37.0	37.0
15	36.449	37.0	37.0	37.0	37.0	37.0
16	36.48625	37.0	37.0	37.0	37.0	37.0
17	36.64475	37.0	37.0	37.0	37.0	37.0
18	36.482	37.0	37.0	37.0	37.0	37.0
19	36.653	37.0	37.0	37.0	37.0	37.0
20	36.70975	37.0	37.0	37.0	37.0	37.0
21	36.67025	37.0	37.0	37.0	37.0	37.0
22	36.4215	37.0	37.0	37.0	37.0	37.0
23	36.49275	37.0	37.0	37.0	37.0	37.0
24	36.549	37.0	37.0	37.0	37.0	37.0
25	36.68075	37.0	37.0	37.0	37.0	37.0
26	36.456	37.0	37.0	37.0	37.0	37.0
27	36.44575	37.0	37.0	37.0	37.0	37.0
28	36.494	37.0	37.0	37.0	37.0	37.0
29	36.45175	37.0	37.0	37.0	37.0	37.0
30	36.5205	37.0	37.0	37.0	37.0	37.0
31	36.54775	37.0	37.0	37.0	37.0	37.0
32	36.56075	37.0	37.0	37.0	37.0	37.0
33	36.62275	37.0	37.0	37.0	37.0	37.0
34	36.5915	37.0	37.0	37.0	37.0	37.0
35	36.63325	37.0	37.0	37.0	37.0	37.0
36	36.60225	37.0	37.0	37.0	37.0	37.0
37	36.41325	37.0	37.0	37.0	37.0	37.0
38	36.0165	37.0	37.0	37.0	37.0	37.0
39	36.14225	37.0	37.0	37.0	37.0	37.0
40	36.22275	37.0	37.0	37.0	37.0	37.0
41	36.0095	37.0	37.0	37.0	37.0	37.0
42	36.533	37.0	37.0	37.0	37.0	37.0
43	36.62525	37.0	37.0	37.0	37.0	37.0
44	36.59575	37.0	37.0	37.0	37.0	37.0
45	36.44275	37.0	37.0	37.0	37.0	37.0
46	36.537	37.0	37.0	37.0	37.0	37.0
47	36.37375	37.0	37.0	37.0	37.0	37.0
48	36.42275	37.0	37.0	37.0	37.0	37.0
49	36.26725	37.0	37.0	37.0	37.0	37.0
50	36.4285	37.0	37.0	37.0	37.0	37.0
51	36.47075	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	0.0
21	5.0
22	4.0
23	3.0
24	1.0
25	8.0
26	4.0
27	3.0
28	5.0
29	5.0
30	15.0
31	30.0
32	47.0
33	56.0
34	113.0
35	329.0
36	1720.0
37	1650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	3.4750000000000005	17.175	71.42500000000001	7.925
2	25.921251892983342	20.318021201413426	14.740030287733468	39.02069661786976
3	12.925	38.4	23.400000000000002	25.275
4	25.7	24.65	15.9	33.75
5	27.3	39.425	22.475	10.8
6	45.7	14.825	24.525	14.95
7	16.925	30.825000000000003	28.9	23.35
8	40.0	22.95	20.974999999999998	16.075
9	15.2	32.475	42.55	9.775
10	12.225	45.95	15.525	26.3
11	28.4	5.35	22.225	44.025
12	21.099999999999998	13.475000000000001	19.725	45.7
13	28.025	17.25	38.875	15.85
14	34.425	30.525000000000002	21.7	13.350000000000001
15	37.025000000000006	25.900000000000002	25.900000000000002	11.175
16	8.625	20.1	25.974999999999998	45.300000000000004
17	16.5	18.55	51.5	13.450000000000001
18	26.150000000000002	11.25	29.049999999999997	33.550000000000004
19	29.975	17.275	41.975	10.775
20	33.85	17.474999999999998	34.525	14.149999999999999
21	15.85	13.750000000000002	55.55	14.85
22	27.775	14.7	53.27499999999999	4.25
23	32.425	14.875	46.1	6.6000000000000005
24	60.95	11.15	25.55	2.35
25	54.725	31.55	11.700000000000001	2.025
26	24.9	60.45	11.924999999999999	2.725
27	8.924999999999999	53.825	33.15	4.1000000000000005
28	4.3	24.325	63.324999999999996	8.05
29	3.925	8.05	60.724999999999994	27.3
30	5.325	2.65	49.15	42.875
31	11.375	1.275	44.25	43.1
32	34.225	1.0999999999999999	21.9	42.775
33	67.625	1.225	10.25	20.9
34	81.075	1.35	8.674999999999999	8.9
35	66.85	1.625	25.825	5.7
36	50.6	2.85	36.5	10.05
37	45.2	4.875	18.55	31.374999999999996
38	22.475	10.2	6.950000000000001	60.375
39	11.275	32.0	2.45	54.275
40	11.25	61.875	1.775	25.1
41	31.924999999999997	57.3	1.4000000000000001	9.375
42	60.724999999999994	33.225	1.325	4.725
43	54.400000000000006	38.625	1.95	5.025
44	25.35	62.64999999999999	3.025	8.975
45	9.0	55.300000000000004	7.1	28.599999999999998
46	3.6999999999999997	25.6	25.1	45.6
47	2.8000000000000003	10.975	36.675000000000004	49.55
48	3.75	9.65	19.25	67.35
49	7.5249999999999995	26.400000000000002	8.924999999999999	57.15
50	25.2	37.7	8.375	28.725
51	36.775000000000006	21.825	25.6	15.8
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	0.5
31	0.0
32	0.5
33	1.0
34	1.0
35	1.0
36	2.0
37	3.0
38	4.0
39	5.0
40	7.0
41	9.0
42	27.5
43	46.0
44	252.5
45	459.0
46	443.0
47	427.0
48	560.0
49	693.0
50	865.5
51	1038.0
52	868.5
53	699.0
54	490.5
55	282.0
56	225.0
57	168.0
58	124.0
59	80.0
60	63.0
61	46.0
62	29.0
63	12.0
64	16.5
65	21.0
66	13.5
67	6.0
68	3.5
69	1.0
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.95
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.474999999999998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90071485305798	23.575
2	8.18109610802224	5.1499999999999995
3	4.447974583002383	4.2
4	3.2565528196981735	4.1000000000000005
5	1.2708498808578237	2.0
6	0.9531374106433679	1.7999999999999998
7	1.0325655281969817	2.275
8	0.6354249404289118	1.6
9	0.6354249404289118	1.7999999999999998
>10	3.971405877680699	27.35
>50	0.3971405877680699	8.525
>100	0.3177124702144559	17.625
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	272	6.800000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	171	4.275	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	152	3.8	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	110	2.75	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	81	2.025	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	72	1.7999999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	70	1.7500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	52	1.3	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	50	1.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	49	1.225	RNA PCR Primer, Index 1 (100% over 27bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	47	1.175	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	46	1.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	40	1.0	RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	26	0.65	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	25	0.625	RNA PCR Primer, Index 1 (100% over 31bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	25	0.625	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	23	0.575	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	20	0.5	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	19	0.475	RNA PCR Primer, Index 1 (100% over 30bp)
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	19	0.475	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGGAGAGAAAGGCAGTTCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GAAGTTGTTCGTGGTGGATTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
ATCACATTGCCAGGGATTTCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TTAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GCTCGACTCATGGTTTGAACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ATCACATTGCCAGGGATTACCACTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGCGGGGCTAGGGCTAACAGCATGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TACAGTACTGTGATAACTGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
ACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.075	0.0	0.0	0.0
2	0.0	0.075	0.0	0.0	0.0
3	0.0	0.075	0.0	0.0	0.0
4	0.0	0.075	0.0	0.0	0.0
5	0.0	0.075	0.0	0.0	0.0
6	0.0	0.075	0.0	0.0	0.0
7	0.0	0.075	0.0	0.0	0.0
8	0.0	0.075	0.0	0.0	0.0
9	0.0	0.075	0.0	0.0	0.0
10	0.0	0.075	0.0	0.0	0.0
11	0.0	0.125	0.0	0.0	0.0
12	0.0	0.15	0.0	0.0	0.0
13	0.0	0.15	0.0	0.0	0.0
14	0.0	0.175	0.0	0.0	0.0
15	0.0	0.4	0.0	0.0	0.0
16	0.0	0.65	0.0	0.0	0.0
17	0.0	1.05	0.0	0.0	0.0
18	0.0	1.7	0.0	0.0	0.0
19	0.0	2.775	0.0	0.0	0.0
20	0.0	5.275	0.0	0.0	0.0
21	0.0	11.375	0.0	0.0	0.0
22	0.0	35.225	0.0	0.0	0.0
23	0.0	70.45	0.0	0.0	0.0
24	0.0	88.125	0.0	0.0	0.0
25	0.0	94.1	0.0	0.0	0.0
26	0.0	95.625	0.0	0.0	0.0
27	0.0	96.425	0.0	0.0	0.0
28	0.0	96.65	0.0	0.0	0.0
29	0.0	96.775	0.0	0.0	0.0
30	0.0	97.05	0.0	0.0	0.0
31	0.0	97.15	0.0	0.0	0.0
32	0.0	97.35	0.0	0.0	0.0
33	0.0	97.625	0.0	0.0	0.0
34	0.0	97.85	0.0	0.0	0.0
35	0.0	98.0	0.0	0.0	0.0
36	0.0	98.025	0.0	0.0	0.0
37	0.0	98.125	0.0	0.0	0.0
38	0.0	98.175	0.0	0.0	0.0
39	0.0	98.2	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAATC	20	5.8554666E-4	45.544304	1
GAGGTAG	70	0.0	45.544304	2
TCGGATC	30	1.5333462E-6	45.544304	1
CGGATCC	30	1.5333462E-6	45.544304	2
CCCTGAG	25	3.0068217E-5	45.544304	2
TGAGGTA	60	0.0	45.544304	1
CTTTGGT	35	7.759627E-8	45.544304	2
TCTTTGG	35	7.759627E-8	45.544304	1
TCCCTGA	25	3.0068217E-5	45.544304	1
TGAGACC	25	3.246753E-5	44.975002	5
ACAGTGA	35	8.606912E-8	44.975002	3
ACCGGTC	35	8.606912E-8	44.975002	10
ATCCTCG	20	6.2400557E-4	44.975002	8
GTATGGT	20	6.2400557E-4	44.975002	15
GGTTGTA	30	1.6779322E-6	44.975002	11
TCCTCGA	20	6.2400557E-4	44.975002	9
TGGTTAT	35	8.606912E-8	44.975002	5
AACCGGT	35	8.606912E-8	44.975002	9
CTGAGCT	30	1.6779322E-6	44.975002	11
TCGACTG	20	6.2400557E-4	44.975002	12
>>END_MODULE
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
Read 2566885 spots for SRR34296799.sra
Written 2566885 spots for SRR34296799.sra
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
Read 2566882 spots for SRR34296799.sra
Written 2566882 spots for SRR34296799.sra
SRR ids: ['SRR34296799.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_glgqze4s
SRR34296799.sra spots: 20535059
blocks: [[1, 2566882], [2566883, 5133764], [5133765, 7700646], [7700647, 10267528], [10267529, 12834410], [12834411, 15401292], [15401293, 17968174], [17968175, 20535059]]
SRR34296799 file size 3548944
SRR34296799 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR34296799 SRR34296799_1.fastq
Input file:	SRR34296799_1.fastq
trimmed:	SRR34296799-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Thu Feb 19 09:20:03 2026 >> started

Thu Feb 19 09:20:10 2026 >> done (6.741s)
20535059 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
     141 ( 0.00%) empty reads filtered out after trimming by size control
20534918 (100.00%) reads available; of these:
     333 ( 0.00%) trimmed reads available after processing
20534585 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 50	     333	  0.00%
 51	20534585	100.00%
20534918 reads passed initial QC


criterion=sequence-density
sequence-density=98.37
sequence-density-rank=1
fanout-score=16.68
fanout-score-rank=1
prefix-density=98.52
prefix-fanout=16.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCACGATCTGG


criterion=fanout-score
sequence-density=98.37
sequence-density-rank=1
fanout-score=16.68
fanout-score-rank=1
prefix-density=98.52
prefix-fanout=16.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCACGATCTGG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCACGATCTGG -o SRR34296799 -
Input file:	STDIN
trimmed:	SRR34296799-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCACGATCTGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Thu Feb 19 09:20:32 2026 >> started

Thu Feb 19 09:20:43 2026 >> done (11.333s)
20120071 reads processed; of these:
  310978 ( 1.55%) short reads filtered out after trimming by size control
   10414 ( 0.05%) empty reads filtered out after trimming by size control
19798679 (98.40%) reads available; of these:
19742167 (99.71%) trimmed reads available after processing
   56512 ( 0.29%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  210363	  1.06%
 19	  533971	  2.70%
 20	 1196630	  6.04%
 21	 4684951	 23.66%
 22	 7237005	 36.55%
 23	 3766191	 19.02%
 24	 1252107	  6.32%
 25	  329308	  1.66%
 26	  126672	  0.64%
 27	   81749	  0.41%
 28	   55318	  0.28%
 29	   32008	  0.16%
 30	   24246	  0.12%
 31	   28491	  0.14%
 32	   34918	  0.18%
 33	   52437	  0.26%
 34	   17719	  0.09%
 35	   12179	  0.06%
 36	   10561	  0.05%
 37	   11022	  0.06%
 38	    6848	  0.03%
 39	    5251	  0.03%
 40	    5833	  0.03%
 41	    4630	  0.02%
 42	    4393	  0.02%
 43	    3782	  0.02%
 44	    3910	  0.02%
 45	    6367	  0.03%
 46	    1374	  0.01%
 47	     713	  0.00%
 48	    1220	  0.01%
 49	       0	  0.00%
 50	       1	  0.00%
 51	   56511	  0.29%


criterion=sequence-density
sequence-density=9.63
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=9
prefix-density=0.00
prefix-fanout=1.0
sequence=TCTTTGGTTATCTAGCTGTATGAAA


criterion=fanout-score
sequence-density=0.63
sequence-density-rank=21
fanout-score=9.14
fanout-score-rank=1
prefix-density=5.66
prefix-fanout=1.0
sequence=GTAGTAGGTTGCATAGTTT
                                 Started job on |	Feb 19 09:20:54
                             Started mapping on |	Feb 19 09:20:54
                                    Finished on |	Feb 19 09:22:07
       Mapping speed, Million of reads per hour |	996.83

                          Number of input reads |	20213526
                      Average input read length |	22
                                    UNIQUE READS:
                   Uniquely mapped reads number |	9376392
                        Uniquely mapped reads % |	46.39%
                          Average mapped length |	22.01
                       Number of splices: Total |	12600
            Number of splices: Annotated (sjdb) |	6534
                       Number of splices: GT/AG |	12412
                       Number of splices: GC/AG |	135
                       Number of splices: AT/AC |	10
               Number of splices: Non-canonical |	43
                      Mismatch rate per base, % |	0.12%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.45
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.03
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	9989195
             % of reads mapped to multiple loci |	49.42%
        Number of reads mapped to too many loci |	103864
             % of reads mapped to too many loci |	0.51%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.82%
                     % of reads unmapped: other |	0.86%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	847939	847939	847939
N_multimapping	9989195	9989195	9989195
N_noFeature	286252	311280	7651806
N_ambiguous	3305855	1868935	40369
UnstrandedReadsAssigned:5784285 PositiveStrandReadsAssigned:7196177 NegativeStrandReadsAssigned:1684217
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR34296799 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR34296799-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 20,213,526 reads, 2,296,810 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 947 rounds

 109283 SRR34296799.ke.tsv
  52636 SRR34296799.se.tsv
 161919 total
==> SRR34296799.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR34296799.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	27
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR34296799 completed mapping pipeline successfully
