Starting /dee2/code/volunteer_pipeline.sh SRR34296815
    current disk space = 244806024
    free memory = 196056048 
SRR34296815 SRAfilesize
7f4b4da84b90f235a8f7a1cb7c2551e6  SRR34296815.sra
SRR34296815.sra file validated
SRR34296815 is single end
SRR34296815 is conventional basespace
SRR34296815 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR34296815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.6585	37.0	37.0	37.0	20.0	37.0
2	34.767	37.0	37.0	37.0	20.0	37.0
3	35.19325	37.0	37.0	37.0	37.0	37.0
4	35.573	37.0	37.0	37.0	37.0	37.0
5	35.88325	37.0	37.0	37.0	37.0	37.0
6	35.731	37.0	37.0	37.0	37.0	37.0
7	36.13925	37.0	37.0	37.0	37.0	37.0
8	36.33425	37.0	37.0	37.0	37.0	37.0
9	35.83625	37.0	37.0	37.0	37.0	37.0
10	36.20525	37.0	37.0	37.0	37.0	37.0
11	36.47025	37.0	37.0	37.0	37.0	37.0
12	36.296	37.0	37.0	37.0	37.0	37.0
13	36.36525	37.0	37.0	37.0	37.0	37.0
14	36.11075	37.0	37.0	37.0	37.0	37.0
15	36.25275	37.0	37.0	37.0	37.0	37.0
16	36.37575	37.0	37.0	37.0	37.0	37.0
17	36.455	37.0	37.0	37.0	37.0	37.0
18	36.326	37.0	37.0	37.0	37.0	37.0
19	36.5365	37.0	37.0	37.0	37.0	37.0
20	36.59725	37.0	37.0	37.0	37.0	37.0
21	36.4865	37.0	37.0	37.0	37.0	37.0
22	36.32025	37.0	37.0	37.0	37.0	37.0
23	36.3135	37.0	37.0	37.0	37.0	37.0
24	36.4805	37.0	37.0	37.0	37.0	37.0
25	36.60925	37.0	37.0	37.0	37.0	37.0
26	36.28575	37.0	37.0	37.0	37.0	37.0
27	36.3195	37.0	37.0	37.0	37.0	37.0
28	36.2795	37.0	37.0	37.0	37.0	37.0
29	36.3465	37.0	37.0	37.0	37.0	37.0
30	36.4555	37.0	37.0	37.0	37.0	37.0
31	36.455	37.0	37.0	37.0	37.0	37.0
32	36.343	37.0	37.0	37.0	37.0	37.0
33	36.46725	37.0	37.0	37.0	37.0	37.0
34	36.39825	37.0	37.0	37.0	37.0	37.0
35	36.53075	37.0	37.0	37.0	37.0	37.0
36	36.437	37.0	37.0	37.0	37.0	37.0
37	36.40075	37.0	37.0	37.0	37.0	37.0
38	35.83275	37.0	37.0	37.0	37.0	37.0
39	36.105	37.0	37.0	37.0	37.0	37.0
40	36.1675	37.0	37.0	37.0	37.0	37.0
41	35.95025	37.0	37.0	37.0	37.0	37.0
42	36.38125	37.0	37.0	37.0	37.0	37.0
43	36.28625	37.0	37.0	37.0	37.0	37.0
44	36.459	37.0	37.0	37.0	37.0	37.0
45	36.2395	37.0	37.0	37.0	37.0	37.0
46	36.38175	37.0	37.0	37.0	37.0	37.0
47	36.228	37.0	37.0	37.0	37.0	37.0
48	36.31125	37.0	37.0	37.0	37.0	37.0
49	36.12625	37.0	37.0	37.0	37.0	37.0
50	36.181	37.0	37.0	37.0	37.0	37.0
51	36.284	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	2.0
21	6.0
22	11.0
23	7.0
24	6.0
25	6.0
26	4.0
27	7.0
28	7.0
29	13.0
30	16.0
31	19.0
32	47.0
33	77.0
34	146.0
35	335.0
36	1633.0
37	1655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.9250000000000003	16.400000000000002	73.2	7.475
2	24.792139077853363	19.47593852355757	13.45427059712774	42.277651801461325
3	12.425	36.125	24.8	26.650000000000002
4	24.075	24.224999999999998	16.625	35.075
5	24.65	38.7	26.575	10.075000000000001
6	47.85	14.399999999999999	22.1	15.65
7	17.299999999999997	31.05	27.650000000000002	24.0
8	38.9	22.225	22.1	16.775000000000002
9	15.325	33.300000000000004	41.75	9.625
10	13.075000000000001	44.824999999999996	13.850000000000001	28.249999999999996
11	26.35	5.975	23.25	44.425
12	19.650000000000002	12.75	17.974999999999998	49.625
13	26.924999999999997	16.05	40.725	16.3
14	34.025	33.050000000000004	20.575	12.35
15	35.8	27.85	25.275	11.075
16	9.35	19.375	23.575	47.699999999999996
17	14.799999999999999	18.4	53.725	13.075000000000001
18	27.575	10.225	29.225	32.975
19	30.525000000000002	14.774999999999999	42.65	12.049999999999999
20	31.55	18.95	36.975	12.525
21	16.475	13.925	54.400000000000006	15.2
22	28.325	14.825	52.0	4.8500000000000005
23	31.6	15.7	45.775	6.925000000000001
24	60.224999999999994	11.65	25.825	2.3
25	54.37499999999999	30.45	12.75	2.4250000000000003
26	26.474999999999998	59.099999999999994	11.625	2.8000000000000003
27	9.625	53.6	31.45	5.325
28	4.3999999999999995	25.324999999999996	62.224999999999994	8.05
29	3.975	8.5	60.12499999999999	27.400000000000002
30	6.125	2.5	49.325	42.05
31	11.325000000000001	1.425	44.074999999999996	43.175000000000004
32	33.75	1.825	21.725	42.699999999999996
33	65.8	1.95	10.625	21.625
34	80.5	1.825	8.875	8.799999999999999
35	66.5	2.4	25.1	6.0
36	50.7	3.2750000000000004	36.075	9.950000000000001
37	44.75	5.075	19.8	30.375000000000004
38	22.45	10.225	7.449999999999999	59.875
39	11.450000000000001	31.4	2.825	54.325
40	11.5	60.425	1.625	26.450000000000003
41	30.75	57.25	1.8499999999999999	10.15
42	59.425	34.025	2.4	4.15
43	54.6	38.025	1.775	5.6000000000000005
44	26.075	61.0	3.6999999999999997	9.225
45	9.475	54.65	6.875000000000001	28.999999999999996
46	4.0	26.650000000000002	24.675	44.675
47	2.375	11.75	36.05	49.825
48	4.375	9.1	19.525000000000002	67.0
49	7.55	25.650000000000002	9.625	57.175
50	24.525	37.175000000000004	7.825	30.475
51	36.075	22.35	24.65	16.925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	1.0
22	1.0
23	0.5
24	0.0
25	0.0
26	0.5
27	1.0
28	0.5
29	0.0
30	0.0
31	0.0
32	0.5
33	1.0
34	1.0
35	1.0
36	3.5
37	6.0
38	6.0
39	6.0
40	7.5
41	9.0
42	26.0
43	43.0
44	256.5
45	470.0
46	437.0
47	404.0
48	521.5
49	639.0
50	829.5
51	1020.0
52	883.0
53	746.0
54	518.0
55	290.0
56	229.5
57	169.0
58	135.0
59	101.0
60	71.5
61	42.0
62	29.0
63	16.0
64	18.5
65	21.0
66	15.5
67	10.0
68	6.0
69	2.0
70	1.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.775
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.3
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28434504792332	22.625
2	11.022364217252397	6.9
3	4.233226837060703	3.975
4	2.8753993610223643	3.5999999999999996
5	1.4376996805111821	2.25
6	1.6773162939297124	3.15
7	0.6389776357827476	1.4000000000000001
8	0.4792332268370607	1.2
9	0.4792332268370607	1.35
>10	3.993610223642172	24.625
>50	0.5591054313099041	10.975
>100	0.3194888178913738	17.95
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	258	6.45	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	182	4.55	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	148	3.6999999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	130	3.25	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	94	2.35	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	75	1.875	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	60	1.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	55	1.375	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	52	1.3	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	52	1.3	RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	51	1.275	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	50	1.25	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	47	1.175	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	45	1.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	43	1.075	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	34	0.8500000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	28	0.7000000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	26	0.65	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	26	0.65	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	25	0.625	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	20	0.5	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 31bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	16	0.4	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 31bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	12	0.3	RNA PCR Primer, Index 2 (100% over 34bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTCC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 27bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAATCCTAGCCCTAGCCCTATGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTAATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GTACAGTACTGTGATAACTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
AGCAGCATTGTACAGGGCTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGCCCGAGTGCCTGCCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATCACATTGCCAGGGATTACCACTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGGGCTAGGGCTAACAGCATGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CTTTTTGCGGTCTGGGCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGGTAGACTATGGAACGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCATCACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TATGTAACACGGTCCACTAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCGTACCGTGAGTAATAATGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACCGTGGCTTTCGATTGTTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TACAGTACTGTGATAACTGAATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTACTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.025	0.0	0.0	0.0
7	0.0	0.025	0.0	0.0	0.0
8	0.0	0.025	0.0	0.0	0.0
9	0.0	0.025	0.0	0.0	0.0
10	0.0	0.025	0.0	0.0	0.0
11	0.0	0.075	0.0	0.0	0.0
12	0.0	0.275	0.0	0.0	0.0
13	0.0	0.375	0.0	0.0	0.0
14	0.0	0.575	0.0	0.0	0.0
15	0.0	0.925	0.0	0.0	0.0
16	0.0	1.175	0.0	0.0	0.0
17	0.0	1.675	0.0	0.0	0.0
18	0.0	2.6	0.0	0.0	0.0
19	0.0	3.45	0.0	0.0	0.0
20	0.0	6.1	0.0	0.0	0.0
21	0.0	11.775	0.0	0.0	0.0
22	0.0	34.925	0.0	0.0	0.0
23	0.0	69.25	0.0	0.0	0.0
24	0.0	87.25	0.0	0.0	0.0
25	0.0	93.475	0.0	0.0	0.0
26	0.0	94.875	0.0	0.0	0.0
27	0.0	95.225	0.0	0.0	0.0
28	0.0	95.45	0.0	0.0	0.0
29	0.0	95.95	0.0	0.0	0.0
30	0.0	96.1	0.0	0.0	0.0
31	0.0	96.25	0.0	0.0	0.0
32	0.0	96.525	0.0	0.0	0.0
33	0.0	96.725	0.0	0.0	0.0
34	0.0	97.025	0.0	0.0	0.0
35	0.0	97.175	0.0	0.0	0.0
36	0.0	97.225	0.0	0.0	0.0
37	0.0	97.35	0.0	0.0	0.0
38	0.0	97.425	0.0	0.0	0.0
39	0.0	97.475	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTTAT	45	2.2009772E-10	45.000004	5
TCTAGCT	45	2.2009772E-10	45.000004	11
ATCTAGC	45	2.2009772E-10	45.000004	10
TTATCTA	45	2.2009772E-10	45.000004	8
TAGCTGT	45	2.2009772E-10	45.000004	13
GGTTATC	45	2.2009772E-10	45.000004	6
GCTGTAT	45	2.2009772E-10	45.000004	15
TATCTAG	45	2.2009772E-10	45.000004	9
CTAGCTG	45	2.2009772E-10	45.000004	12
TTTGGTT	45	2.2009772E-10	45.000004	3
TTGGTTA	45	2.2009772E-10	45.000004	4
CTTTGGT	45	2.2009772E-10	45.000004	2
AGCTGTA	45	2.2009772E-10	45.000004	14
GTTATCT	45	2.2009772E-10	45.000004	7
CTTTTGG	40	4.3564796E-9	45.0	18
TTATGGA	20	6.222983E-4	45.0	20
ACAGTGA	75	0.0	45.0	3
ACCGGTC	75	0.0	45.0	10
GAGGTAG	35	8.569259E-8	45.0	2
AACCGGT	75	0.0	45.0	9
>>END_MODULE
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
Read 2524621 spots for SRR34296815.sra
Written 2524621 spots for SRR34296815.sra
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
Read 2524615 spots for SRR34296815.sra
Written 2524615 spots for SRR34296815.sra
SRR ids: ['SRR34296815.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_j0lgirqy
SRR34296815.sra spots: 20196926
blocks: [[1, 2524615], [2524616, 5049230], [5049231, 7573845], [7573846, 10098460], [10098461, 12623075], [12623076, 15147690], [15147691, 17672305], [17672306, 20196926]]
SRR34296815 file size 3490360
SRR34296815 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR34296815 SRR34296815_1.fastq
Input file:	SRR34296815_1.fastq
trimmed:	SRR34296815-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Thu Feb 19 09:18:16 2026 >> started

Thu Feb 19 09:18:23 2026 >> done (6.662s)
20196926 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
     421 ( 0.00%) empty reads filtered out after trimming by size control
20196505 (100.00%) reads available; of these:
     402 ( 0.00%) trimmed reads available after processing
20196103 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       1	  0.00%
 19	       0	  0.00%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	     401	  0.00%
 51	20196103	100.00%
20196505 reads passed initial QC


criterion=sequence-density
sequence-density=97.54
sequence-density-rank=1
fanout-score=16.70
fanout-score-rank=1
prefix-density=97.81
prefix-fanout=16.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCCTGATCTGGGGG


criterion=fanout-score
sequence-density=97.54
sequence-density-rank=1
fanout-score=16.70
fanout-score-rank=1
prefix-density=97.81
prefix-fanout=16.7
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCCTGATCTGGGGG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCCTGATCTGGGGG -o SRR34296815 -
Input file:	STDIN
trimmed:	SRR34296815-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCCTGATCTGGGGG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Thu Feb 19 09:18:39 2026 >> started

Thu Feb 19 09:18:49 2026 >> done (10.596s)
19784332 reads processed; of these:
  495048 ( 2.50%) short reads filtered out after trimming by size control
     578 ( 0.00%) empty reads filtered out after trimming by size control
19288706 (97.49%) reads available; of these:
19116816 (99.11%) trimmed reads available after processing
  171890 ( 0.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  197420	  1.02%
 19	  510184	  2.64%
 20	 1168585	  6.06%
 21	 4463258	 23.14%
 22	 6929896	 35.93%
 23	 3638709	 18.86%
 24	 1226746	  6.36%
 25	  308800	  1.60%
 26	  115857	  0.60%
 27	   82828	  0.43%
 28	   67460	  0.35%
 29	   44040	  0.23%
 30	   37411	  0.19%
 31	   46453	  0.24%
 32	   59645	  0.31%
 33	   82582	  0.43%
 34	   23559	  0.12%
 35	   14991	  0.08%
 36	   12496	  0.06%
 37	   14027	  0.07%
 38	    8406	  0.04%
 39	    6842	  0.04%
 40	    8738	  0.05%
 41	    7056	  0.04%
 42	    6140	  0.03%
 43	    6058	  0.03%
 44	    6693	  0.03%
 45	   13681	  0.07%
 46	    2502	  0.01%
 47	    2015	  0.01%
 48	    3739	  0.02%
 49	       0	  0.00%
 50	       1	  0.00%
 51	  171888	  0.89%


criterion=sequence-density
sequence-density=9.52
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=9
prefix-density=0.00
prefix-fanout=1.0
sequence=TCTTTGGTTATCTAGCTGTATGAAA


criterion=fanout-score
sequence-density=0.70
sequence-density-rank=22
fanout-score=8.25
fanout-score-rank=1
prefix-density=5.70
prefix-fanout=1.0
sequence=GTAGTAGGTTGCATAGTTT
                                 Started job on |	Feb 19 09:18:58
                             Started mapping on |	Feb 19 09:18:59
                                    Finished on |	Feb 19 09:20:01
       Mapping speed, Million of reads per hour |	1143.92

                          Number of input reads |	19700879
                      Average input read length |	23
                                    UNIQUE READS:
                   Uniquely mapped reads number |	9134017
                        Uniquely mapped reads % |	46.36%
                          Average mapped length |	22.25
                       Number of splices: Total |	8543
            Number of splices: Annotated (sjdb) |	2540
                       Number of splices: GT/AG |	8389
                       Number of splices: GC/AG |	102
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	50
                      Mismatch rate per base, % |	0.13%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.66
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.05
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	9683324
             % of reads mapped to multiple loci |	49.15%
        Number of reads mapped to too many loci |	101613
             % of reads mapped to too many loci |	0.52%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.39%
                     % of reads unmapped: other |	0.58%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	883538	883538	883538
N_multimapping	9683324	9683324	9683324
N_noFeature	280635	303304	7430943
N_ambiguous	3345978	1925024	34669
UnstrandedReadsAssigned:5507404 PositiveStrandReadsAssigned:6905689 NegativeStrandReadsAssigned:1668405
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR34296815 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR34296815-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 19,700,879 reads, 2,223,923 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 950 rounds

 109283 SRR34296815.ke.tsv
  52636 SRR34296815.se.tsv
 161919 total
==> SRR34296815.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR34296815.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	4
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR34296815 completed mapping pipeline successfully
