Starting /dee2/code/volunteer_pipeline.sh SRR34296825
    current disk space = 260722420
    free memory = 195705336 
SRR34296825 SRAfilesize
06818744ba866b891ed883e911ba5193  SRR34296825.sra
SRR34296825.sra file validated
SRR34296825 is single end
SRR34296825 is conventional basespace
SRR34296825 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR34296825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47525	37.0	20.0	37.0	12.0	37.0
2	34.54575	37.0	37.0	37.0	20.0	37.0
3	35.3335	37.0	37.0	37.0	37.0	37.0
4	34.93	37.0	37.0	37.0	20.0	37.0
5	35.10775	37.0	37.0	37.0	37.0	37.0
6	35.38575	37.0	37.0	37.0	37.0	37.0
7	35.90075	37.0	37.0	37.0	37.0	37.0
8	35.88	37.0	37.0	37.0	37.0	37.0
9	35.7675	37.0	37.0	37.0	37.0	37.0
10	36.26425	37.0	37.0	37.0	37.0	37.0
11	36.187	37.0	37.0	37.0	37.0	37.0
12	36.28025	37.0	37.0	37.0	37.0	37.0
13	36.23075	37.0	37.0	37.0	37.0	37.0
14	36.13025	37.0	37.0	37.0	37.0	37.0
15	36.3855	37.0	37.0	37.0	37.0	37.0
16	35.79925	37.0	37.0	37.0	37.0	37.0
17	36.367	37.0	37.0	37.0	37.0	37.0
18	36.45325	37.0	37.0	37.0	37.0	37.0
19	36.21325	37.0	37.0	37.0	37.0	37.0
20	36.16975	37.0	37.0	37.0	37.0	37.0
21	36.06175	37.0	37.0	37.0	37.0	37.0
22	36.05925	37.0	37.0	37.0	37.0	37.0
23	36.1445	37.0	37.0	37.0	37.0	37.0
24	36.18575	37.0	37.0	37.0	37.0	37.0
25	36.359	37.0	37.0	37.0	37.0	37.0
26	36.0385	37.0	37.0	37.0	37.0	37.0
27	35.87275	37.0	37.0	37.0	37.0	37.0
28	36.139	37.0	37.0	37.0	37.0	37.0
29	36.27625	37.0	37.0	37.0	37.0	37.0
30	36.088	37.0	37.0	37.0	37.0	37.0
31	36.212	37.0	37.0	37.0	37.0	37.0
32	35.95825	37.0	37.0	37.0	37.0	37.0
33	36.36175	37.0	37.0	37.0	37.0	37.0
34	36.33325	37.0	37.0	37.0	37.0	37.0
35	36.2955	37.0	37.0	37.0	37.0	37.0
36	36.2835	37.0	37.0	37.0	37.0	37.0
37	36.073	37.0	37.0	37.0	37.0	37.0
38	36.204	37.0	37.0	37.0	37.0	37.0
39	35.81425	37.0	37.0	37.0	37.0	37.0
40	35.87825	37.0	37.0	37.0	37.0	37.0
41	36.00775	37.0	37.0	37.0	37.0	37.0
42	36.19775	37.0	37.0	37.0	37.0	37.0
43	36.1185	37.0	37.0	37.0	37.0	37.0
44	36.202	37.0	37.0	37.0	37.0	37.0
45	35.9375	37.0	37.0	37.0	37.0	37.0
46	36.1275	37.0	37.0	37.0	37.0	37.0
47	36.23575	37.0	37.0	37.0	37.0	37.0
48	36.1205	37.0	37.0	37.0	37.0	37.0
49	36.055	37.0	37.0	37.0	37.0	37.0
50	35.81075	37.0	37.0	37.0	37.0	37.0
51	36.00525	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	3.0
21	10.0
22	9.0
23	7.0
24	14.0
25	7.0
26	7.0
27	5.0
28	9.0
29	14.0
30	27.0
31	39.0
32	66.0
33	106.0
34	159.0
35	510.0
36	1852.0
37	1154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.324999999999999	16.2	69.27499999999999	10.2
2	25.99186164801628	18.71820956256358	14.572736520854527	40.71719226856562
3	13.5	36.125	23.849999999999998	26.525
4	25.874999999999996	22.05	17.175	34.9
5	26.674999999999997	38.5	23.799999999999997	11.025
6	46.300000000000004	14.399999999999999	23.599999999999998	15.7
7	19.025	31.574999999999996	26.75	22.650000000000002
8	40.725	21.3	20.674999999999997	17.299999999999997
9	15.525	32.2	41.425	10.85
10	13.125	44.45	15.825	26.6
11	29.175	5.525	21.224999999999998	44.074999999999996
12	19.900000000000002	13.55	18.65	47.9
13	26.55	16.150000000000002	40.1	17.2
14	32.95	30.099999999999998	22.725	14.224999999999998
15	36.95	24.875	25.15	13.025
16	9.0	18.95	26.150000000000002	45.9
17	15.9	20.225	50.14999999999999	13.725000000000001
18	27.250000000000004	10.174999999999999	29.875	32.7
19	31.574999999999996	15.35	41.05	12.025
20	33.95	17.325	35.699999999999996	13.025
21	17.375	14.35	53.225	15.049999999999999
22	26.875	15.0	52.675000000000004	5.45
23	30.975	13.725000000000001	47.575	7.725
24	59.0	11.799999999999999	25.8	3.4000000000000004
25	52.925	29.349999999999998	13.775	3.95
26	24.375	57.525000000000006	13.55	4.55
27	11.0	51.7	31.574999999999996	5.7250000000000005
28	7.225	22.475	61.35	8.95
29	7.5249999999999995	8.3	58.525000000000006	25.650000000000002
30	8.649999999999999	3.85	45.425	42.075
31	12.6	2.5	45.0	39.900000000000006
32	33.225	2.475	21.525	42.775
33	65.35	2.45	11.1	21.099999999999998
34	76.7	3.05	9.625	10.625
35	64.425	3.925	24.25	7.3999999999999995
36	48.05	5.2749999999999995	36.225	10.45
37	46.325	6.825	17.549999999999997	29.299999999999997
38	23.45	10.925	7.175	58.45
39	12.775	31.0	3.775	52.449999999999996
40	12.15	60.650000000000006	2.6	24.6
41	30.25	55.325	2.9749999999999996	11.450000000000001
42	58.425000000000004	31.0	3.4000000000000004	7.175
43	52.6	36.6	2.8000000000000003	8.0
44	24.125	60.62499999999999	3.6999999999999997	11.55
45	10.225	54.225	7.1	28.449999999999996
46	5.45	25.55	24.15	44.85
47	4.55	11.774999999999999	37.175000000000004	46.5
48	5.0	10.525	18.2	66.27499999999999
49	7.449999999999999	26.674999999999997	9.225	56.65
50	23.45	38.425	8.975	29.15
51	35.75	20.925	24.75	18.575
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	1.5
31	3.0
32	2.0
33	1.0
34	1.0
35	1.0
36	2.5
37	4.0
38	5.0
39	6.0
40	7.0
41	8.0
42	25.5
43	43.0
44	223.0
45	403.0
46	412.5
47	422.0
48	537.5
49	653.0
50	789.5
51	926.0
52	841.5
53	757.0
54	518.0
55	279.0
56	245.0
57	211.0
58	166.5
59	122.0
60	96.0
61	70.0
62	49.0
63	28.0
64	36.0
65	44.0
66	28.5
67	13.0
68	9.0
69	5.0
70	2.5
71	0.0
72	0.0
73	0.0
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.7000000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5	27.900000000000002
2	9.097222222222221	6.550000000000001
3	3.6111111111111107	3.9
4	2.1527777777777777	3.1
5	0.9027777777777777	1.625
6	0.6944444444444444	1.5
7	0.625	1.575
8	0.6944444444444444	2.0
9	0.4861111111111111	1.575
>10	3.5416666666666665	25.624999999999996
>50	0.4166666666666667	10.05
>100	0.2777777777777778	14.6
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	227	5.675	RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	127	3.175	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	117	2.9250000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	113	2.825	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAGT	89	2.225	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	86	2.15	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	66	1.6500000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCAG	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	52	1.3	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	51	1.275	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	50	1.25	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	47	1.175	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	46	1.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	45	1.125	RNA PCR Primer, Index 1 (100% over 31bp)
TAACAGTCTACAGCCATGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	43	1.075	RNA PCR Primer, Index 1 (100% over 29bp)
TAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTACACTCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	35	0.8750000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
TTTGAACCATCACTCGACTCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 30bp)
AGAGGTAGTAGGTTGCATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	25	0.625	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTATGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	19	0.475	RNA PCR Primer, Index 1 (100% over 31bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCCA	19	0.475	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TAACAGTCTACAGCCATGGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGATCTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
ACTAATCCTAGCCCTAGCCCTACATGGAATTCTCGGGTGCCAAGGAACTCC	16	0.4	RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	16	0.4	RNA PCR Primer, Index 1 (100% over 31bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
TTTGAACCATCACTCGACTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
TCTTTGGTTATCTAGCTGTATGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 30bp)
CTGGCCCTCTCTGCCCTTCCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TCTACAGTGCACGTGTCTCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	13	0.325	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13	0.325	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCGAGGAGCTCACAGTCTAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 30bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	10	0.25	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	10	0.25	RNA PCR Primer, Index 2 (100% over 34bp)
CTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
ACTAATCCTAGCCCTAGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
TTAAGGCACGCGGTGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CTAGCACCATCTGAAATCGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GAAGTTGTTCGTGGTGGATTCGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
TGAGGTAGTAGTTTGTGCTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGCGGGGCTAGGGCTAACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
AACCCGTAGATCCGATCTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
ATAAAGCTAGATAACCGAAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
CTATACGACCTGCTGCCTTTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 32bp)
ACTAATCCTAGCCCTAGCCCTACACTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCCCTTACCCCAAAAAGCATTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGGAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGAGAAAGGCAGTTCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TAAGGCACGCGGTGAATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGGTTGTATAGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTAATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATCATGATGGGCTCCTCGGTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TTGTGCTTGATCTAACCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AAGGGATTCTGATGTTGGTCACACTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TATGCAAGGGCAAGCTCTCTTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
ACCGTGGCTTTCGATTGTTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGATCTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
AAGCCCTTACCCCAAAAAGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.025	0.0	0.0	0.0
8	0.0	0.025	0.0	0.0	0.0
9	0.0	0.025	0.0	0.0	0.0
10	0.0	0.05	0.0	0.0	0.0
11	0.0	0.075	0.0	0.0	0.0
12	0.0	0.175	0.0	0.0	0.0
13	0.0	0.475	0.0	0.0	0.0
14	0.0	0.6	0.0	0.0	0.0
15	0.0	1.1	0.0	0.0	0.0
16	0.0	1.7	0.0	0.0	0.0
17	0.0	2.75	0.0	0.0	0.0
18	0.0	4.525	0.0	0.0	0.0
19	0.0	6.15	0.0	0.0	0.0
20	0.0	8.65	0.0	0.0	0.0
21	0.0	14.225	0.0	0.0	0.0
22	0.0	35.7	0.0	0.0	0.0
23	0.0	69.875	0.0	0.0	0.0
24	0.0	85.725	0.0	0.0	0.0
25	0.0	91.425	0.0	0.0	0.0
26	0.0	93.25	0.0	0.0	0.0
27	0.0	94.2	0.0	0.0	0.0
28	0.0	94.95	0.0	0.0	0.0
29	0.0	95.425	0.0	0.0	0.0
30	0.0	95.65	0.0	0.0	0.0
31	0.0	95.9	0.0	0.0	0.0
32	0.0	96.1	0.0	0.0	0.0
33	0.0	96.6	0.0	0.0	0.0
34	0.0	97.025	0.0	0.0	0.0
35	0.0	97.175	0.0	0.0	0.0
36	0.0	97.175	0.0	0.0	0.0
37	0.0	97.325	0.0	0.0	0.0
38	0.0	97.5	0.0	0.0	0.0
39	0.0	97.575	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGA	65	0.0	45.000004	3
ACCGGTC	65	0.0	45.000004	10
AACCGGT	65	0.0	45.000004	9
GGTCTCT	65	0.0	45.000004	13
GAACCGG	65	0.0	45.000004	8
GTCTCTT	65	0.0	45.000004	14
GGTAGTA	45	2.2009772E-10	45.000004	4
AGTGAAC	65	0.0	45.000004	5
GTGAACC	65	0.0	45.000004	6
CGGTCTC	65	0.0	45.000004	12
TGAACCG	65	0.0	45.000004	7
TCACAGT	65	0.0	45.000004	1
CACAGTG	65	0.0	45.000004	2
CAGTGAA	65	0.0	45.000004	4
GTAGTAG	45	2.2009772E-10	45.000004	5
CCGGTCT	65	0.0	45.000004	11
GAGGTAG	50	1.0913936E-11	45.0	2
TAAACAT	40	4.3564796E-9	45.0	3
TTGTATA	25	3.236105E-5	45.0	13
AAACATC	40	4.3564796E-9	45.0	4
>>END_MODULE
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
Read 2883687 spots for SRR34296825.sra
Written 2883687 spots for SRR34296825.sra
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
Read 2883683 spots for SRR34296825.sra
Written 2883683 spots for SRR34296825.sra
SRR ids: ['SRR34296825.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_dla8jiob
SRR34296825.sra spots: 23069468
blocks: [[1, 2883683], [2883684, 5767366], [5767367, 8651049], [8651050, 11534732], [11534733, 14418415], [14418416, 17302098], [17302099, 20185781], [20185782, 23069468]]
SRR34296825 file size 3988340
SRR34296825 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR34296825 SRR34296825_1.fastq
Input file:	SRR34296825_1.fastq
trimmed:	SRR34296825-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Thu Feb 19 09:10:23 2026 >> started

Thu Feb 19 09:10:28 2026 >> done (5.764s)
23069468 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
     150 ( 0.00%) empty reads filtered out after trimming by size control
23069318 (100.00%) reads available; of these:
23069318 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 51	23069318	100.00%
23069318 reads passed initial QC


criterion=sequence-density
sequence-density=97.65
sequence-density-rank=1
fanout-score=16.64
fanout-score-rank=1
prefix-density=97.98
prefix-fanout=16.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCT


criterion=fanout-score
sequence-density=97.65
sequence-density-rank=1
fanout-score=16.64
fanout-score-rank=1
prefix-density=97.98
prefix-fanout=16.6
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCT -o SRR34296825 -
Input file:	STDIN
trimmed:	SRR34296825-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Thu Feb 19 09:10:44 2026 >> started

Thu Feb 19 09:10:55 2026 >> done (10.740s)
22598516 reads processed; of these:
  947195 ( 4.19%) short reads filtered out after trimming by size control
     872 ( 0.00%) empty reads filtered out after trimming by size control
21650449 (95.80%) reads available; of these:
21539626 (99.49%) trimmed reads available after processing
  110823 ( 0.51%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  369189	  1.71%
 19	  626048	  2.89%
 20	 1295596	  5.98%
 21	 4831953	 22.32%
 22	 7679997	 35.47%
 23	 3877304	 17.91%
 24	 1413575	  6.53%
 25	  419571	  1.94%
 26	  195387	  0.90%
 27	  146910	  0.68%
 28	  113455	  0.52%
 29	   79798	  0.37%
 30	   62848	  0.29%
 31	   65669	  0.30%
 32	   72637	  0.34%
 33	  103400	  0.48%
 34	   30363	  0.14%
 35	   21897	  0.10%
 36	   19145	  0.09%
 37	   19316	  0.09%
 38	   13698	  0.06%
 39	   11287	  0.05%
 40	   12766	  0.06%
 41	   10251	  0.05%
 42	    8605	  0.04%
 43	    8165	  0.04%
 44	    8373	  0.04%
 45	   14421	  0.07%
 46	    3275	  0.02%
 47	    1940	  0.01%
 48	    2787	  0.01%
 49	       0	  0.00%
 50	       0	  0.00%
 51	  110823	  0.51%


criterion=sequence-density
sequence-density=8.41
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=11
prefix-density=0.00
prefix-fanout=1.0
sequence=TCTTTGGTTATCTAGCTGTATGAAA


criterion=fanout-score
sequence-density=0.91
sequence-density-rank=17
fanout-score=7.20
fanout-score-rank=1
prefix-density=6.51
prefix-fanout=1.0
sequence=TAGTAGGTTGTGTGGTTTTT
                                 Started job on |	Feb 19 09:11:04
                             Started mapping on |	Feb 19 09:11:04
                                    Finished on |	Feb 19 09:12:08
       Mapping speed, Million of reads per hour |	1244.32

                          Number of input reads |	22121251
                      Average input read length |	23
                                    UNIQUE READS:
                   Uniquely mapped reads number |	9780014
                        Uniquely mapped reads % |	44.21%
                          Average mapped length |	22.21
                       Number of splices: Total |	12243
            Number of splices: Annotated (sjdb) |	2696
                       Number of splices: GT/AG |	12014
                       Number of splices: GC/AG |	144
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	83
                      Mismatch rate per base, % |	0.15%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.40
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	10921468
             % of reads mapped to multiple loci |	49.37%
        Number of reads mapped to too many loci |	222857
             % of reads mapped to too many loci |	1.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.47%
                     % of reads unmapped: other |	1.94%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1419769	1419769	1419769
N_multimapping	10921468	10921468	10921468
N_noFeature	396237	435632	8071822
N_ambiguous	3509008	2119418	48700
UnstrandedReadsAssigned:5874769 PositiveStrandReadsAssigned:7224964 NegativeStrandReadsAssigned:1659492
Dataset is classified unstranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR34296825 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 109,282
[index] number of k-mers: 92,755,666
[index] number of equivalence classes: 769,752
[quant] running in single-end mode
[quant] will process file 1: SRR34296825-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,121,251 reads, 2,599,355 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,103 rounds

 109283 SRR34296825.ke.tsv
  52636 SRR34296825.se.tsv
 161919 total
==> SRR34296825.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSMUST00000177564.1	16	4.59059	0	0
ENSMUST00000196221.1	9	3.70903	0	0
ENSMUST00000179664.1	11	4.02702	0	0
ENSMUST00000178537.1	12	4.16231	0	0
ENSMUST00000178862.1	14	4.39563	0	0
ENSMUST00000179520.1	11	4.02702	0	0
ENSMUST00000179883.1	16	4.59059	0	0
ENSMUST00000195858.1	10	3.87667	0	0
ENSMUST00000179932.1	12	4.16231	0	0

==> SRR34296825.se.tsv <==
ENSMUSG00000102693	0
ENSMUSG00000064842	0
ENSMUSG00000051951	6
ENSMUSG00000102851	0
ENSMUSG00000103377	0
ENSMUSG00000104017	0
ENSMUSG00000103025	0
ENSMUSG00000089699	0
ENSMUSG00000103201	0
ENSMUSG00000103147	0
SRR34296825 completed mapping pipeline successfully
